LOCUS       DACEMO010000001        55295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEMO010000001 DACEMO010000000
VERSION     DACEMO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.001"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            426..1556
                     /locus_tag="IW728_000001"
     CDS             426..1556
                     /locus_tag="IW728_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1023392.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FNDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1648..2403
                     /locus_tag="IW728_000002"
     CDS             1648..2403
                     /locus_tag="IW728_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1023393.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2461..4500
                     /locus_tag="IW728_000003"
     CDS             2461..4500
                     /locus_tag="IW728_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1023394.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4609..5760)
                     /locus_tag="IW728_000004"
     CDS             complement(4609..5760)
                     /locus_tag="IW728_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1023395.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5914..6444)
                     /locus_tag="IW728_000005"
     CDS             complement(5914..6444)
                     /locus_tag="IW728_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023396.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6543..6893)
                     /locus_tag="IW728_000006"
     CDS             complement(6543..6893)
                     /locus_tag="IW728_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1023397.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(7050..8579)
                     /gene="ahpF"
                     /locus_tag="IW728_000007"
     CDS             complement(7050..8579)
                     /gene="ahpF"
                     /locus_tag="IW728_000007"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1023398.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8589..9152)
                     /gene="ahpC"
                     /locus_tag="IW728_000008"
     CDS             complement(8589..9152)
                     /gene="ahpC"
                     /locus_tag="IW728_000008"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1023399.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9296..9958)
                     /locus_tag="IW728_000009"
     CDS             complement(9296..9958)
                     /locus_tag="IW728_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1023400.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            10142..11788
                     /locus_tag="IW728_000010"
     CDS             10142..11788
                     /locus_tag="IW728_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1023401.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11779..12861
                     /gene="uxuA"
                     /locus_tag="IW728_000011"
     CDS             11779..12861
                     /gene="uxuA"
                     /locus_tag="IW728_000011"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1023402.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(12969..14522)
                     /locus_tag="IW728_000012"
                     /pseudo
     CDS             complement(12969..14522)
                     /locus_tag="IW728_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            14670..15811
                     /locus_tag="IW728_000013"
                     /pseudo
     CDS             14670..15811
                     /locus_tag="IW728_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(15937..17016)
                     /locus_tag="IW728_000014"
     CDS             complement(15937..17016)
                     /locus_tag="IW728_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1023403.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(17119..17559)
                     /locus_tag="IW728_000015"
     CDS             complement(17119..17559)
                     /locus_tag="IW728_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1023404.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(17616..18026)
                     /locus_tag="IW728_000016"
     CDS             complement(17616..18026)
                     /locus_tag="IW728_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1023405.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(18206..18640)
                     /locus_tag="IW728_000017"
     CDS             complement(18206..18640)
                     /locus_tag="IW728_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1023406.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(18740..19588)
                     /locus_tag="IW728_000018"
     CDS             complement(18740..19588)
                     /locus_tag="IW728_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1023407.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            19977..21743
                     /locus_tag="IW728_000019"
     CDS             19977..21743
                     /locus_tag="IW728_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1023408.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            21845..23104
                     /locus_tag="IW728_000020"
     CDS             21845..23104
                     /locus_tag="IW728_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1023409.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            23251..24552
                     /locus_tag="IW728_000021"
     CDS             23251..24552
                     /locus_tag="IW728_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1023410.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            24555..25406
                     /locus_tag="IW728_000022"
     CDS             24555..25406
                     /locus_tag="IW728_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1023411.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            25419..26234
                     /locus_tag="IW728_000023"
     CDS             25419..26234
                     /locus_tag="IW728_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1023412.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            26385..27551
                     /locus_tag="IW728_000024"
     CDS             26385..27551
                     /locus_tag="IW728_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1023413.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            27781..28428
                     /locus_tag="IW728_000025"
     CDS             27781..28428
                     /locus_tag="IW728_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1023414.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            28512..29456
                     /locus_tag="IW728_000026"
     CDS             28512..29456
                     /locus_tag="IW728_000026"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1023415.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            29591..31042
                     /locus_tag="IW728_000027"
     CDS             29591..31042
                     /locus_tag="IW728_000027"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1023416.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            31063..31899
                     /locus_tag="IW728_000028"
     CDS             31063..31899
                     /locus_tag="IW728_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1023417.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            31899..32558
                     /locus_tag="IW728_000029"
     CDS             31899..32558
                     /locus_tag="IW728_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1023418.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            32684..33373
                     /locus_tag="IW728_000030"
     CDS             32684..33373
                     /locus_tag="IW728_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023419.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            33379..34335
                     /locus_tag="IW728_000031"
     CDS             33379..34335
                     /locus_tag="IW728_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1023420.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            34379..34891
                     /locus_tag="IW728_000032"
     CDS             34379..34891
                     /locus_tag="IW728_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1023421.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(35147..35695)
                     /locus_tag="IW728_000033"
     CDS             complement(35147..35695)
                     /locus_tag="IW728_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1023422.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(35784..36965)
                     /locus_tag="IW728_000034"
     CDS             complement(35784..36965)
                     /locus_tag="IW728_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1023423.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(37201..38709)
                     /locus_tag="IW728_000035"
     CDS             complement(37201..38709)
                     /locus_tag="IW728_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1023424.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            38868..39059
                     /locus_tag="IW728_000036"
     CDS             38868..39059
                     /locus_tag="IW728_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023425.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            39262..40179
                     /locus_tag="IW728_000037"
     CDS             39262..40179
                     /locus_tag="IW728_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1023426.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            40172..41245
                     /locus_tag="IW728_000038"
     CDS             40172..41245
                     /locus_tag="IW728_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023427.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            41393..41911
                     /locus_tag="IW728_000039"
     CDS             41393..41911
                     /locus_tag="IW728_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023428.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            41892..42284
                     /locus_tag="IW728_000040"
     CDS             41892..42284
                     /locus_tag="IW728_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1023429.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            42360..43295
                     /locus_tag="IW728_000041"
     CDS             42360..43295
                     /locus_tag="IW728_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1023430.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            43428..44207
                     /locus_tag="IW728_000042"
     CDS             43428..44207
                     /locus_tag="IW728_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1023431.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            44200..44517
                     /locus_tag="IW728_000043"
     CDS             44200..44517
                     /locus_tag="IW728_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1023432.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            44761..46371
                     /locus_tag="IW728_000044"
     CDS             44761..46371
                     /locus_tag="IW728_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1023433.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            46528..47376
                     /locus_tag="IW728_000045"
     CDS             46528..47376
                     /locus_tag="IW728_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1023434.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            47607..48086
                     /locus_tag="IW728_000046"
     CDS             47607..48086
                     /locus_tag="IW728_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023435.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            48119..50347
                     /locus_tag="IW728_000047"
     CDS             48119..50347
                     /locus_tag="IW728_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1023436.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            50476..52119
                     /locus_tag="IW728_000048"
     CDS             50476..52119
                     /locus_tag="IW728_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1023437.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(52192..52737)
                     /locus_tag="IW728_000049"
     CDS             complement(52192..52737)
                     /locus_tag="IW728_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1023438.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(52760..53245)
                     /locus_tag="IW728_000050"
     CDS             complement(52760..53245)
                     /locus_tag="IW728_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1023439.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(53235..54047)
                     /locus_tag="IW728_000051"
     CDS             complement(53235..54047)
                     /locus_tag="IW728_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1023440.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(54186..>55295)
                     /locus_tag="IW728_000052"
     CDS             complement(54186..>55295)
                     /locus_tag="IW728_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1023441.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        18319 a        10021 c        10704 g        16251 t
ORIGIN      
        1 tatttttttc taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat
       61 tgcctaatca ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg
      121 ttaggtgagg ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg
      181 ggtaaaacag aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg
      241 ttgtatagtg ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa
      301 ggtttctgac cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc
      361 ctcacctgac aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt
      421 ggattttggg tccttatttg actttgtcca tgatcggtct tttagaagta tacagtgcca
      481 gttcttatcg tttattgcaa gccgatgaaa atacaaaaag tctattattg cgtcaactaa
      541 ttttcatatt tttgagttgg agcgtgatct tcttagctcg ttcagtcaaa ctacactatt
      601 tacttcaccc taaaatagca ggatacggtt tagccttatc gattttcttt ttagtattag
      661 taagaatagg gatattcggt gtcactgtca acggcgcaca acgttggatc tctctgtttg
      721 gcattcaatt ccagccttct gaactggcaa atcttttttt gattttttat ttaagctggt
      781 tttttcgtga cggaaatagt agcccaaaag atctaaaaaa accattcctg attacagtag
      841 gtataacttt tctgatttta tttcagccaa agattgctgg agcattgatg atcctttcga
      901 ttgcgtgggt catattttgg gcagcggcgg ttccatttaa aaaagggatc tatctaatcg
      961 ttactttttc tgcattgctg attggagcag caggcggggt attatattta ggaaataaag
     1021 gttggcttcc acagatgttt aatcatgctt atgaaaggat cgcgacttta agagattctt
     1081 ttaacgatag tcatggtgcg ggctatcaaa tgacccattc gttttatgca ctatataatg
     1141 gtggcatatt tgggcgggga ttaggaaata gtatcacaaa aaaagggtat cttccagaga
     1201 cagaaacaga ttttattttt tcgattatta cagaagagct tgggctaatc ggtgcattgt
     1261 gtgtgctttt cttattgttc tcattatgca tgagaatctt ttgtttaagc agtcgctgta
     1321 aaaatcagca agctggattg tttcttttag gattcggaac tttgctattc gttcagacga
     1381 taatgaacgt gggaagtatc gcggggctga tgccaatgac tggtgtgcca ttgccatttg
     1441 ttagctacgg aggaacaagt tatctgatcc tttctttagg aatcggaatc acgttgaata
     1501 tttcttcaaa gatacaggcc gaagagttgg cgctctatcg acctgaaaaa caataaagag
     1561 aaactgagaa actctcagtt tgaattatca taaagtacaa tcttttattt ttcaagcgag
     1621 aggttctttt agaaagagga attcgcaatg aaagtatctc aaaaaatcat attatctatt
     1681 ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca tggattttcg
     1741 tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc taccacacta
     1801 aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg ctcggattca
     1861 cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc tatcatgaga
     1921 gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc ttactcttat
     1981 gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga agctccctat
     2041 tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt tcctggtgga
     2101 gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa caaaggagag
     2161 caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga caaagaagga
     2221 gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca gcagctgaaa
     2281 gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg tagtatccaa
     2341 acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa agacatatgt
     2401 tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa ggaggaaaaa
     2461 atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc cggcgcagtg
     2521 atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca agaaacccaa
     2581 gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa caagggagat
     2641 tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc attatctgaa
     2701 aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa aggacttcag
     2761 atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc atataaagca
     2821 aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac attagacaga
     2881 aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga aatggaagga
     2941 aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat agatcgaaat
     3001 ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc aagtaaactt
     3061 ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt cgacttaaca
     3121 gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta ctttgtccca
     3181 ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat tcaagaagta
     3241 gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta cgtgggagat
     3301 atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa aatcggtcgc
     3361 tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg gaaattaagc
     3421 attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt tcaaaatggc
     3481 aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga cagtctagga
     3541 ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct tgcgcttgct
     3601 agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga agattacaaa
     3661 gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg ttatgctcct
     3721 ggatctacgt ttaaaatgat tacagcagca atcggtctcg acaacggcac tatcgatcca
     3781 aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg gggatcttat
     3841 caagcaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc tttgatctat
     3901 tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa atttcgtaca
     3961 ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat gaatccagca
     4021 caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac tggatatgga
     4081 cagggcgaac ttctaattac tcctatccag caagcagcaa tgtattctgt ttttaccaac
     4141 aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga taagaagaat
     4201 gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt tgtgcaagat
     4261 gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc gaaaactggt
     4321 acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt cttgtttgct
     4381 ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa agaagatgat
     4441 gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa ttatcaataa
     4501 aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt ccttttttca
     4561 aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc acacttcata
     4621 aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta ataaaatcac
     4681 gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc caatttgtaa
     4741 aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca atgaactatg
     4801 ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg tcaaaatcgc
     4861 tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa aatcaacttc
     4921 taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag cctctacatt
     4981 gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa aaaaagcacc
     5041 aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac aaataactag
     5101 cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa gtaggaaagg
     5161 cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt caatcaaaac
     5221 aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc tgaaacttag
     5281 aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg atccttcttt
     5341 tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag aagttgccgc
     5401 aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt tgcctgtcaa
     5461 ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac caggtcggaa
     5521 atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta ataagatcac
     5581 accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca atccagcttg
     5641 gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta atctccgcga
     5701 gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa taaattccat
     5761 tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca ttattgaaaa
     5821 aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg gattgttggt
     5881 ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt ttcttattct
     5941 gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga atatttggaa
     6001 tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa tcaacaacag
     6061 aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga acaactggaa
     6121 caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa cgattgcagg
     6181 tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga acagaccgat
     6241 actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca acaacccaag
     6301 caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag aagggtttag
     6361 cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt atttttgaat
     6421 attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt taacgtgaac
     6481 tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata tctctggaaa
     6541 aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc gatcatttta
     6601 tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg tacaccacaa
     6661 acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc aaaaaattgt
     6721 tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata acccgtcagc
     6781 caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc ttttgctaca
     6841 tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc cataatcagt
     6901 cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag agaagaaaaa
     6961 gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat ccttttttcc
     7021 gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc gaatgctcct
     7081 aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg actatctgta
     7141 caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc ggtcactatt
     7201 tcatttcttg gattcatttc gatcgttcca tctagccaat ccgtattagg gagtaagccg
     7261 actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg gcgatcgata
     7321 taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc atgtttgata
     7381 acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc ttttaattct
     7441 ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat tgctgcttca
     7501 acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa agggccgtca
     7561 cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg tacattgata
     7621 gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga tataccgtta
     7681 gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg ttgattcatc
     7741 ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa ttttggacct
     7801 tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac ttgtccgccg
     7861 aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat agctgcacta
     7921 ctagctgctg gtccaccgcc aatcactaat acatcataag gttctttttc aaacaactct
     7981 tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat gcgtccgcta
     8041 ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt atcatcgatt
     8101 tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt taatacagct
     8161 aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct aacataactg
     8221 tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc actgattttc
     8281 ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc atgaccgagg
     8341 gggataccag caaaaacgat tccactttct ttgccaactg gattgataga aaaactaggt
     8401 gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc tatttccttc
     8461 gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga aaaaacaata
     8521 ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt gtctaacata
     8581 tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc gccactttct
     8641 ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc agcgcgaatc
     8701 ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc catagcttga
     8761 acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg ctcttcatcc
     8821 aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac gtattcgatt
     8881 ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt atcggtagag
     8941 caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg taagtcttct
     9001 aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat actccagttc
     9061 cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc atgtgcatta
     9121 aattcttcga tttttttccc aatcaatgac atagtaaaac ctccaataat aattatttat
     9181 ttataattat tgtaatttag ttggagttgc aaagtcaatg gatattttgt tatttaataa
     9241 agattactca aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga aaatgctact
     9301 gctggaaata ctcgggataa cgttgagtaa cagtttcctg ttctttcagc atcatgtgta
     9361 agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt atcaactaaa gccttgagta
     9421 aatcataatg ttgttccatg atcgtttccc atggaagccc ctgaatggtc actcgtaacc
     9481 agcggaaacg ttctaaatgg gtattatggt catctaacca gttccaaact tcttttcttc
     9541 ctgcaatttc gaaacaagct tcatgaaata aattatctgc acggaaaaaa gcgcgttcgt
     9601 catttatttt gactgctttt tcttgttctt ctaaaatcgt ttctagcact attcgtgttt
     9661 gtgtgtccaa tttggaacaa cattcaatca tgatttgctc ttccaactgt tctcggacaa
     9721 agcgagcatt ttctgcagaa acaagattaa ttttggaaac atacgtacca ctttgaggta
     9781 tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg ggagtccggc
     9841 cgatagcaag atgttcctcc agtgcctttt cagatatttt ccttccaggt tctaagtcag
     9901 catagataat ttgtttacga atcgcttgat aagcttgtga ttgcaagctg cttttcatct
     9961 ttattcctcc aaaatcattt ctatgaacgt agtataacag aagaggtctg aaaaacaatc
    10021 tggtatgtca cattaactag taaatttgta taaatagtaa aattttggtt gacatagcga
    10081 tttttattgc ataaaattca cttgaataca aaaactaata tgttagttta ggaggggaaa
    10141 aatgattgat ttaaacaact ggaggaactt tatccctgaa ttatctcaaa aagaagtaga
    10201 aatacctcat tttgaccgtg catcattaag agaaaatgga caaaaaaatc ctgtctggat
    10261 ccatttcggt ggcggaaatc tttatcgagg atttcatgca gaaatcgctc aacgtttagc
    10321 ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg aagaaataat
    10381 tgaaagggct taccaagatt ttgatgattt gattcttgaa gtggtcatgc caagcagtgg
    10441 agagttcgtc aaacgtatat tagctgcgac agctgacagt ctgtattgtc atccagattg
    10501 ttctgaacac tttgataaga tgattcgaca ctttgaaaat cctagtttac aattagttac
    10561 ttttgctatt actgaaaaag gttatcgatt gaaagataca aataataaat atataggacc
    10621 gattgcagtc gatataaaaa atggtccaaa gaagccatca catacaatga gtattcttgc
    10681 tgtgctactt tataaacgtt ttttatctgg agcttggccg attgcacttg tctctacaga
    10741 taatttttca caaaacggta gtcaattacg tacttctttg ctaacgatcg caaaagaatg
    10801 gcaaaaggaa ggttatgtac aaaatacatt tatccaatac ttatcgaatg aagaaattgt
    10861 tgcgtttcca tggacgatga ttgatcgtat caccccgaat ccttccttgt cagttagcaa
    10921 aatgttggca aaagagggct tttcaaatat gtcgattttc caaaccgcaa aaggaaataa
    10981 tattgctcca tttgtcaata cagaggacat ccattattta atcatggaag atcattttcc
    11041 caatggacgt ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg caacggtaga
    11101 taaagcagat acgatgaagg tgacgacttg ccttaatcct ttgcatacgt ctttagctat
    11161 tatgggttgt ttactggggt ataggagcat agctgaggag atgaaagatc cagatttatc
    11221 gctgctagtt tctgaaatcg ggtacaatga aggcctccca gttgtggagt ctccaggtat
    11281 cattgatcca aagatctttc tggaagaagt aattactgtg cgcttttcta atccaaatat
    11341 tcctaatatg cctcagcgaa tcgccacgga tacctctcaa aaggtggcga ttcgttatgg
    11401 tgagacgatt aaaaaatata tggaaataga agttaaagac ccgcagacac ttgtattcat
    11461 accattgact ctcgctgctt ggcttcgata ccttattggc attgatgatc aaggaaaacc
    11521 attcatcccg agtccagacc cactattgac agaccttcag cataagcttt ccgatattca
    11581 actgggtacg caagatacat cacatattca tgagaaaatg gctcctattt tgtcaaataa
    11641 agaaattttt ggtgtagatc tttaccaagc agagcttggc agaaaaatag aagaaatgtt
    11701 catcaagatg gtagcaggac caaatgccgt acgaaaaaca ttacaggatt acttacaaga
    11761 acatggagga aatttatcat ggaaatgact tttagatggt acggatcaca ggaagaaaag
    11821 attaccttag atgaaatcag acagattcct gcggtcaaag ggattgtagg cacattgatg
    11881 gatataccag taggagaggt atggcctagg gagaggatca aagaactaaa agaagaaatt
    11941 gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca atgtgcatga tgccattaaa
    12001 attggattac cttcccgtga gcagtatatc gaaaactata agcagacaat ccgaaacttg
    12061 gcagaatatg gaatagaagt catctgctat aattttatgc ctgtatttga ctgggtgaaa
    12121 tctgaccttg actaccgctt agaagacggc tcatccacgt tagcttttat atctgatgat
    12181 attccagatg atccaagaga aatcgtcgcg cgcatcgaac attccagcaa tgaatttgga
    12241 ttgccgggat gggaacctga gcgcttagct cctatcaaat ccttgcttga agcctatgca
    12301 tcagtagacg aagaaaaact tcgagaaaat tttgcctatt ttctccaatc aatcatccca
    12361 acatgtgaag aggtaggggt caaaatggct gtacatccag atgatccgcc atactcgatt
    12421 tttggattag ctcgaatcgt aaagaatcga gaagatttag attggttgtg tcgcatagta
    12481 gacagccctt ataacggaat cactctttgt acagggagta ttgcagaaga tccagaaaat
    12541 gatgtatatg ggattttagc agaattttgc caaagagatc gaatcccatt tgcacatgtt
    12601 cgaaatatta aatttttagc taatcgagat ttctatgagg caccacataa atccgaatac
    12661 ggttcattag acatgtaccg catactaaaa acaatgtatg acaacggatt taacggatac
    12721 atccgtcctg atcatggaag aatgatctgg aatgagacag gaagaccagg atacggctta
    12781 tatgatcgag cattaggtgc tgtttatcta aatggattat gggaagcaat cgaaaaagga
    12841 aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa taagaaggat
    12901 gttcaaattc ttgatacata aatccggagg ttgttaataa gcattcggat ttatgtatct
    12961 tatttggtct aggaagtaac ttgattttct tttttcgatt gatccatgag tgcttcttct
    13021 tttaattcaa agatatattc tgagatcctc ataatcactt ttttccagtc ttctggtgtg
    13081 atgattcctt ttaaaatata gatttttttg atatgagaag gcaattgttt caaagggcag
    13141 tcgctgataa cgatgttgca gttctccata aaatcatttt gtattttgat cgattcattt
    13201 gaaaaaattt gttctatctt aacgatcaga tctttactga tataaaaatc acgcgtatag
    13261 ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat catacaaatg tactaggtct
    13321 tcaaccatcc attgcacatt ttccttttct aaaataactg gtaacaagtg ttgattttgt
    13381 tctctgaaat ccatataaaa ttgttcaatg gcggtttgga cttctttcaa tacgctatgt
    13441 tcttgataaa gcatgaaagt aggaaggggc gtatttggtt gattgaagtt aagaaatcca
    13501 ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt ttcaggtata
    13561 aacaaatcaa atacagtaca aagggactcc accatttctc ttgaataaag aacatgagga
    13621 acgtttgctt tttttaaaag tttgtattgc tccaagcttg tttcttccga ttcgattttc
    13681 gtgatagaaa gtcgagctaa aatattcact aagatgcgtt gttcgcgaca aattggcaga
    13741 ttaggtcgca aaatatctac agagagatca ataaaaaagt caatatattc atactctgtg
    13801 aattcttttt gtacgtcttt cacagaagtt gcaggaaaat tctctttaca tatttttaat
    13861 agaaaatata atttatctat cataccgcta tttagtcggt caagtacttc ttttttaaaa
    13921 aaagtattca tcataaaagc cactacttgc ggatcttcat cataaaattc tgtagcagta
    13981 cttgaaaaga tgttccaata aatatctaat gctgcatatt gaatttgtac ctcttttcca
    14041 gaaaaagact ttctgccatt attagaaaag gagatagaca aaccgtacta tgctaaaaat
    14101 gcattaatct ttttcatttt cgtatacaaa taagaggcac tatagtttgt ttgctgactg
    14161 aaactgataa tcgaatttgt ctgacagctg aacaaagaag tcaagatctg ataactgact
    14221 gattcgttgc agtatcttcc aaatagacga taatagcagt aagccggatc aattccttca
    14281 aaattcaact ttaaaaaagg tttttgctta tcaatatgca gttctgttag attgagacgc
    14341 tcaaagtctt gttctaattc tttgatgtat agtaaaaaat ttgattcaga catatggaaa
    14401 tgatctgcta aatattgtgt agtaataaag aaacgatgtt cagataggaa aagaaaaata
    14461 tctaattttg tattgctttt tttcttcaag ataaacgttt taatcgcttc ttgagtttgc
    14521 atgaaagagg ctccttttta cttaaattgt taaaacttga aaatattact gtttttttca
    14581 taaatttaga caaaaaaaca gtaaacatat aggtagaatc tctactatct tatttcttgc
    14641 atacaaaaaa atcaaggagt gagagagtta tgagatattc aaccatctta atatcaacat
    14701 ttgcagcctt aagcttatcg cttactataa atcagacagt ccacacagag gagacagaaa
    14761 cagccaaatg gacagaaacc aagtgggcca acgtcaatcg aattaccttt caggatgaat
    14821 tttcatcctt caattcagga gtcgcaaaat taagaaaagg acaaacagcg accatcaaag
    14881 caaaaattga ctattcggga gacttagcac ctatactaca atcagctgtt acatttgaga
    14941 atgttgatcc cactttgtcg attggctatg acgagaacac tttgactatc caacacaagc
    15001 aagcgatatt taccttcacc gttacattaa atcaggactt gttaaaacca gcgcttttta
    15061 ctgtcaaggt atcagatgga ctaccgaacg atcatcattt aacgccgtat agtcagcggc
    15121 agacaattga gcaagattca gccatcgact caggaggaga tcttgttgaa gaaccaacag
    15181 ataaaccaga aaatgaaaat aagccagaag taccgcctac agaaaatcca gacggagaac
    15241 agaaaccgga aatagaacct ggagaagaac ccgatacaga aactcaacca gagccagaca
    15301 ataaatcaaa acctgaaata acccctggag aaaaaccaga tgtaactcca gaaccagata
    15361 cggattctgg aaaccaaaca gtaccggaaa caaatccaga cacagataat gaaactgaaa
    15421 atccagaaaa accagaggta gatccagaag aaaaaccaga tgtgactcca gaaccagata
    15481 cggacgcaag agaccaagga ataccagaaa aaataaacaa aaaaacaatt caagaggatg
    15541 gaaaaaaaga gagtaaaaaa tcaaatctag caattctaaa aataaatgaa gagcagctga
    15601 ataaaaaatc acgaatattt gatagcgccc aatcagctga aaccttaaaa agtagcaagg
    15661 atacaacctt tgctgtcctg aaacgaaaaa taaacagcta ccaaaatccg gagagtcaca
    15721 aaacaaagtt atactgtgga gcggaatcat tctattaagt atagcaacaa tgctttctgc
    15781 aaaaagattt aaacaaaaca gatctctcta acatttgaat aatgtctgtg aacgcatttt
    15841 tcaaataaaa agaaatatcc agaaaatgac cccaaaatac cagattgaaa tctgatattt
    15901 ggggtcgttc atactttatc tttcttcata tttattttaa gggatatctg tttcttttgg
    15961 aagatggttc gggatctttt tcactttcaa tacatctaaa aagaaggtaa agatcggaat
    16021 gccgacaatc aagccccaaa cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt
    16081 ataaaaaatc ggcagttctg ttttgctgga catcaacttc ggattcagca cataagactc
    16141 aattagatgg acaatcaaaa tcgtaatcag gatataaaca acatctcgga tgcctccttg
    16201 ggaataagct atgaaagata gaggaataca agaaatgata acacctgcta ctgggatcag
    16261 acttaaaatg aaaatcatga tcgctaaact cagcagctga tgaaagccaa atacagccaa
    16321 ggcgattgta gtgagcaacg tatttaaaag agcgacaaca aattgagctt ctaataccaa
    16381 accaaatgta ttgacaaact tatcggcaaa atagtaaata tcttgaaaga accaagaaaa
    16441 ttcacttttt aaaaacaatc ttgagaatac aattgttttt ttcttttcaa tcataaaaaa
    16501 gaaacttaag atgaatgaca tagcaaacgc cactgccatt tttcctatat cctgtaaata
    16561 gccaagcgca atcgaagcgc cattttgaag ctgttcaaaa aaatcgtttc gctcaatata
    16621 agtatggata tattgtaaaa caggatctgc atcttttggc tgatgctcgt aaaaattaat
    16681 aacggactgg atcatgtcaa atgtttgatg aataagcatc gggatatact ttgtgaatgc
    16741 cagatagata aggtaaacga tcagtgtata aatcatgata gctaaaacac ctgtagggat
    16801 tcggacaaat cgctgaatga tttgaacaag atgaatcatc aaataagtaa agataaatgt
    16861 gagcaaaatg gttgtaatca tacttctaac taagaaaaga acaaaaacga caaataataa
    16921 aacagtgaga cggcgtattt tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac
    16981 aacctccaaa taacaaaaaa taaacacgtt attcattata tgatggaata tgaaaataag
    17041 aaagagcaaa aagaaaaacc gctaaatgac gttatcttat gagttggttt tcctcataaa
    17101 atcgttcatc tagcagtttc agcccttttt ctggtcttga tgaaaattat ctagcatttt
    17161 tcgggtcaga tgttgcagtg tatccaattc ttcctgttca agtggaagat aagacttgat
    17221 cttttgtgga ataccttctg cttgtttttg caacgcagca ccttgagcag tcagcgtaac
    17281 atttacgatt ctttcatcac ccgtttcacg ctgacgatta atataatttt tttctgctaa
    17341 cttttttagt aagggagtca gcgtaccaga atccagacac aaatcatttc ctagctcttt
    17401 cactgaagtc tttttctttt cccataaaac taacatcaca agatactggg tataggtaag
    17461 attcagcggc ttgaggaggg gagtgtagtg tctaatgatt tctttagagg cggcatataa
    17521 aggaaaacat acttgattaa ctaaatatag atcttccatt tttccacctc cgctgtttta
    17581 ctatagtgta tcatagcgga tttacacaaa aaagtctatt cactaactgc tttcagagta
    17641 acttctatat ttccagctgt agcttttgaa tacggacaca ctttatgcgc tgcctctaat
    17701 aattcttgcg tttcttccag agaaagacct tcgatagatc cttcaatctc tacacctaag
    17761 acaaagctcg tttcactttc tgaataaagg gaaacttgtg cactgacagt agaagcccca
    17821 gagatatttt tttgctgttt gatcaattct aatgcactgt taaaacatgc actgaaacca
    17881 gctgcaaaca attgttcagg gttggttgca ttttcgacgc gtttaccagg agctgcaata
    17941 ttcaattcga atgtacgatc gggattatgc acttctccag agcgtccgcc tgtgttgatc
    18001 attttcgtag aatacatttt tttcataaaa ttaccacctt taattatatt ttatacaatt
    18061 gaattttaca caatataatt gtggaaagca aagaaatgga ttggcataag ataaataaga
    18121 gaaagcacat gaaacagacg cgctttctcg aatagcaaac atatacaaaa aatatcagag
    18181 acaaaaaata atctcatcat aaacattaca tatatttctc aaaaagttgt tttctttctt
    18241 caaaaattac ttcacgatgg acctgatgaa gcatccataa ataaccaaaa ggatctctga
    18301 acattgagtt agtcacacca aagtccatca tttctgtcat ttcttgaatc tctacacagc
    18361 catttcgtaa tgctttatta tgtgtgtcct cgatatcaga gacgatgata ttgaaccaga
    18421 tcgtatttgg atgttcagca gtaggagcag tcaaaccaag agacgtgttt tcatccagca
    18481 tatggaatct cgtaccatac aaggtaaaaa tgacttcatt attgcctcta gggtaatctg
    18541 ttgcctcaat cagttgtatt tcatcaaaga ttgtttgata cagttctagt gctttcaaac
    18601 tatcatttaa tacaaaatcg atttccgttc ctttatgcat aaagatctcc tctccttttc
    18661 agtcgaacag ttactagatt cttttattat accagctaaa gaaaacgcat tcaaaaaaat
    18721 agcggattta cttaccaact cattcgattg gatgataagt aaatccgcgt cccattactt
    18781 ctgctacgct gctgacaatg acaaaggcac ttggatcgat ttcttctatc aatggtttca
    18841 ttcgggaaaa ttcatgatca gtcacaacga tcaataaaac ctcttttgac tttcgattat
    18901 acccgccttt tgcctctagc agggtaaggc cacgtccaat ctcagcactt aagcgaaggc
    18961 ggatgtcttc caaataatgt tcactcatga ccataacaga tttctttcga ttgatacctg
    19021 tttcgatata agtcatgaca attgaagtga ttgcaataga taaaatagca taaagaaact
    19081 cttctattcc aaaaataaac agattaagag aaacaacgaa tgcgtctgta aaaaataagc
    19141 caatcgaagt attcaagtgg aaatattttt taaaaatgag gggagggatc gtcgtaccgc
    19201 cgctagaaga gttgttttta tataaaatcg caactcctaa agcaaaaatc gcacttccaa
    19261 aaatgacaga caacaaccga tctgaagtaa tcattgtttc tggaactatt gcaatgatta
    19321 aaggaaaaac caagcttccg aaaagtactt taaaaaaggg ttttttacct aaaaatagaa
    19381 gagctagtac caacataacg atattcaaca ctaaaataac tatagctcgg tcaaagccta
    19441 aagcagcttc cagtaatatt cctagaccgc tgacacctcc agcagcaata tgatgaggcc
    19501 ctaaaaacat attgatagcg actcctaata tgaagagggc aaaaataatc ttaatgtatg
    19561 tccatataaa ttgtctcgtt tctttcattt attcacttcc ttaattcatt aataatactc
    19621 ttatcttatt ataacttact tttcacctta agttaacttt aattaataaa aaaattaggc
    19681 tagtctaaat tattatgttt cattcttcta gaatatactt ctacgccaaa aactgttaaa
    19741 aaacttttac tttagaaaat tggagaaatt gatcgcttaa gataaattca ggacactaaa
    19801 tagtacagga agtagactaa aacttttaaa agttttaagt ctatctattt ggcaagttta
    19861 aaaaataaaa attcggtatc aaaaaactgt taccgataac atttttgaaa gcgttgtctt
    19921 ttgcttctct atcacttata gtgatgacgt catattaaaa cacaaggagg aaatcaatgg
    19981 atacagctgc aatttatcat cgaccggaaa gtgaatatgc atttctatat acgaatacaa
    20041 gatttcgtat tcggcttcgt actaggaaaa atgatatcaa aaaagtatat gtgctatgtg
    20101 gagatccata taccataaca actgaaaaat ggtatcaaaa acaacgacca atgaaaaaat
    20161 gtctaagtac caatgttcat gattattggg aaattgaagt gacgagcgaa acaagtcgct
    20221 tacagtatgc gttccatgtt gttggggaag ataacacgga ctgtttttac ggtgatcaag
    20281 gaatttttcc ttatcgagaa aatgtgatta ctgaaccaaa tttttatttt cgaatacctt
    20341 attttcatca aatcgatcga ttcactattc cagaatgggt aaaagaaaca gtatggtatc
    20401 aaatttttcc cgaaagattt gcaaacgggg acaaagcaaa tgaccccgaa cacactttac
    20461 cttggggaag taaagatcca gaccgagaag actttttcgg gggagatttg caaggtgtga
    20521 tggatcattt ggattacttg acggaccttg gcatcaatgg catctatttt tgtcccatat
    20581 ttaaagccac atccaatcat aaatacgata cgattgatta ttatgaggtg gatcccgctt
    20641 ttggcgataa aaaactttta aaaaacttga ttgatgaagc acataaaaga gggatacgta
    20701 tcatgttcga tgctgtattc aatcatatgg gtgtccattc accacagtgg caagacgttc
    20761 tcaaaaacgg agaaaaatcg atctataaag actggttcca catccgtttt ttcccagtag
    20821 attcttatca gatgactgac ttaccagaaa ctgctgaaaa tatcccatat gatacttttg
    20881 cttttactcc attcatgccg aaactaaata cagcaaatcc agaagtgcaa aaatatttgc
    20941 tggatatcgc aacttattgg ataaaagaat tcgatattga tggatggcgt cttgatgttg
    21001 ctaatgaagt cgatcatcat ttttggaaaa aattcagaga agcagttaca gaaatcaaac
    21061 cagacatcta tatccttgga gagatttggc attcctctca agcttggctg caaggggatg
    21121 aattccatgc agtgatgaat tatgcattta cagattcgat taaagattat tttgcaaaaa
    21181 agaaaatcac agcttcacaa atggtcagtg gaatgaatca tcagcaaatg ctttacagag
    21241 atcaggtgaa cgaaggaaca ttcaacttat tagattctca tgatactgcc aggatcttga
    21301 ccctttgcca aggtaacaaa gaattgatga agtctgtgat ggcctttatg tttcttcaaa
    21361 aagggtctcc ttgtatctat tacgggacag aaatagggat gactggtgaa gatgacccgg
    21421 attgccgcaa atgtatgata tgggagaagg aggaacaaga tctagagcta ttcggtttta
    21481 taaaagaact tgtttctcta aggaaacagt tgtctaagat tatttctgaa ggttctactc
    21541 agtggctaat agtaaatgat cgtgaagata agctctattt cacacggaaa ctagagggac
    21601 agatcatcta tgtttacttt aatcagtcaa aagaaccttg ggtagtagaa caggaaaatg
    21661 aggtgattct tagtcaaaat tgtcagttgt tagaagacgg gaaagcagag atacgacaat
    21721 acggattttt gatcatgtgt tgaaaactgt taccggtaac aacaagaaag cgtttgcaaa
    21781 tgaagatttg tgcccattat aataagggtg taacagcacg ttgaatacaa aggaggaaat
    21841 gaagatgaaa aaaagaagtg tgaaacagtt gggtttaggg ttaatctctt taggaatctt
    21901 aggcggatta gcggcttgtg gcaatggtaa ctcttcagca gaaggggaag ccgatgaagg
    21961 aaaaacgtta acagtttccg ttgattctgg atataaagat tatataaacg aaataaaaaa
    22021 cactttcgaa aaagaaaacg atgtgaaaat caagctagta gaaaaggaca tgttcgatca
    22081 gttggaatca ttagcgttag atgggcctgc tggaaaggga cctgacgtta tgatggcagc
    22141 atatgaccgg atcggtgcgt taggacagca aggacatctg gcggaagtaa aactgggaaa
    22201 tgaagcagct tacgatgaaa cagacaaagc ccaagtgact tatgatggaa aaatctacgg
    22261 agaacctgca gtaatcgaga cccttgtttt gtactataac aaagatttag tcgatacagc
    22321 accatcaacg tttaaagatt tggagaatct atccaaagac agtcgttttg cttttgagtc
    22381 agaagcagga aaaaatacag gatttttagc taaatggact gacttttatt attcctatgg
    22441 tttaattgca ggttacggtg gctatgtatt tggtgatgat ggcacaaatc cttcagatat
    22501 cggtttgaat aatgcaggtg ctgtagaagg aatctcttat gcaacagatt ggttcaaaaa
    22561 tgtttggcca aagggaatgc aagacataaa aagtgcagga gacttcgcca gccagcaatt
    22621 catgtccaat aaaactgcag caatcattga cggtccatgg caagcgcaaa catacaaaga
    22681 aaacaatatc aattatggtg tagccaaaat tcctactcta aataatggac agccttatca
    22741 accgtttggc ggaggaaaag gatgggtagt cagcaattat gctaaaaaca aagacctctc
    22801 tcaaaaatgg cttgattatg taacgaatca ggaaaatcaa gaaaaattct ttgaaatggt
    22861 aaatgaaatc cctgcaaacc aacaagctag ggaaacagca aaggctaaaa acgatgaatt
    22921 aacaacagct gtgattgaac aatatgaaac agctcaagcg atgccaaaca tcccagaaat
    22981 gggcgaagtt tggacaggtg cagaaaactt gatgttcgat gcagcatcag gttcaaaaac
    23041 accaaaagaa tccgcagatg aagcagtaaa aacaatcagt gaagccatag aacaaaaata
    23101 ctgattaaaa tgataaccgt ccatttttat aaaaaatgga acaggtgctt cagataaaag
    23161 tggcaagagc gagatggaaa attatcttgc tcttgttgcc attatcttaa aagcagtttg
    23221 cggtttagca agtttagagg gggcattcag atgcagcaag ataaaacaag caatatcaac
    23281 gaaaaaaaaa tcacgctttg ttcacttatt ccagggatgg ggcagtttgt aaatggccaa
    23341 gcttttaaag gtatcgtttt tttcgccata ttcgtcctat tcattggtca gatgcttctg
    23401 ggcggatttc atgcattggt aggattggtt actttgggaa gtataccaat ggaagatcat
    23461 tcattattta ttttgattga aggcactttg caattcatca ttacaatcat tttcttgatt
    23521 ttttacggtt tgaatctttt agacgcttat cgagtagcga agatgcgtca agagggcaaa
    23581 aaaatcaatc ggacgatgaa ggaagtcata cataatatag gtgatgaagg ttttccttat
    23641 ctattgacac ttcccgctta tcttctcatg atttttacga tcattttccc agtcctagtt
    23701 acattgttca ctgcatttac taattatgac ttcaatcata ttccaccagc cagtttaatt
    23761 gattgggtag gggctaaaaa cttttttagt atcttctttt taagctcata tcgtaatact
    23821 ttttttgcag tcttttcgtg gaccgtgatt tggactttat gtgcaacaac tttgcaaatc
    23881 acccttggta ttctcacagc ggttgttgct caccagaaat ttattaaatt caaacgtatt
    23941 ttcggtatca ttttcttatt accatgggcg gtcccagctt ttattactat catgagtttt
    24001 tcgaatatat tcaacgatag cgtaggtgcc atcaatactc aagtgattcc tttcataaac
    24061 cagcttcctt ttgtagatat cagtgcaatc tcttggaaaa cggacccgaa ctggacgaaa
    24121 gtagcaatta tcttagtgca gggctggtta ggatttccct atatctatgt gatggttaca
    24181 ggaatcttac aatcgatttc agaagactta tatgaagcag caaaaatcga cggagcaaat
    24241 gcatggcagc gcttttcaaa catcacgctt cctgctgtct tcctagtggc agcgccaaca
    24301 tttattacac agtatacggg gaatttcaat aatttctcca tgatctatct gtttaacaat
    24361 ggcggtcccg gaagcgtagg cggaggagct ggttctactg atattcttat ttcctggatc
    24421 tataaactta cgactggaac ttctccgcag tattctatgg cttcagcaat cacgttgatt
    24481 atctccgtca tcgttatctg tgtgtcattg ctggtattta aaaagacaaa tgcgttcaat
    24541 atgggggatt aacaatgaaa aaatttcatt cgagtgctcg attccaaaaa cgactttctc
    24601 acttttttac ttactttttt cttttaatac tgtcagtcat tatcatctat ccgcttttga
    24661 tcacagcaag tactgccttc aagtcaggaa atattgcagc ttttagttta gatctttcaa
    24721 gtgactggac tttaggaaac tttactcggc tattcagtga aacactttac gggacttggt
    24781 ataaaaatac attgatcatt tctgtcctaa caatgatcgt acaagtgact gtagtaactt
    24841 tggcgggcta tacttatagt cgctatcgtt ttatcggccg caaatcaagt ttgatgttct
    24901 ttttagtgat tcagatggta ccgactatgg cagcattgac tgccttttat gtcatggctt
    24961 tcttattaga agcgctcgat aagtactggt tcttgacaat gatctatata ggcggtggaa
    25021 ttccaatgaa tacttggctg atgaaaggat actttgacac tgtaccaata gatttagatg
    25081 aatctgctaa acttgatggg gcagggcatt tcaggatttt tttccagatt gtattaccgc
    25141 ttgtcaaacc gatgctcgcc gttcaagcat tatgggcttt tatgggacct tttggggatt
    25201 tcatgttagc taagttcctg cttcgaacac ctgaaaatca gactgttgct gtcggcttac
    25261 aaacatttat ttctgatgtg acaaaccaaa gagtctctct ttttgctgct ggggcaattt
    25321 tgattgcact accgatttgt ctattattct tctttttaca aaagaacttt gttaccggcc
    25381 tgacatctgg cggaacaaaa ggataggagc agagtatcat gcaaacattt ccgttaaact
    25441 atttttccgc attacgtacg ccaactcaag tgtttgcggg acgcaaacta ttatcatggc
    25501 ctaaattttt cttgatattt gtatttttag tttccttgat ggtcatgcct gtgactcttt
    25561 tttatgccaa tcagatccaa gcgattccat tagaacaatt tctatccgtt cattcgctga
    25621 tcgatgaaca aggaacccaa aaattttctg aacttgaatt atctgaaact gggttacaag
    25681 catcgcaaca aacgatagct gtgacccctg aaattttggt aggtgtgtct ttatctgaga
    25741 aacaacaatc cgatcatgga acttttattg attttgagaa ggaacaatgg gtgatccaac
    25801 aaaaggataa atctggtata cgccgctata cgatgaatta tagcccatct ttccaaccag
    25861 attctgtacg tacgcccgaa gatttccaac gatttttaga aagagaattt tatgccagta
    25921 atcgtccaac aatcatttta tcttatagtt taagtcttgg tcttgtccta tttgtcatga
    25981 cgagtctcat tttattcggg gcttcctttt tcttgtggat gacaagaaaa agccaacttt
    26041 catccattca tacattcaaa gagagtgcaa atttgatgct gaatgttatg ggaataggtt
    26101 ccatggcagc agcagttgta gggtttatcc actttgactt tatcttgatg ctaagtgtcc
    26161 aaacaacgat aactgtcttg ctgcttttat gggtttttgt caaaacaaga ttcaaagata
    26221 agcgtgtcga atgacaagtt taagcgattt atgcgacaat gaatgtgaaa aaaatgaaaa
    26281 ataaagtagt ttttgaaaaa tttctgaaaa aattaaactt cttagttgat tcttttcttt
    26341 tttagtatta acgtaatagg gtagtttttt taaggaggaa aatcatgtta gagaaaactt
    26401 tatacaatca attattttca cgttctttct cgctccctgt cgaagtaaat tattgggacg
    26461 gtacaacgaa acaatacgga gatacggaca ctcctaccca aatcaaaatc aatatccatg
    26521 aagaaatccc aatgaaagag attacaagta acgcatcttt ggcactagga gaagcctata
    26581 tggatcatcg aatcgagatc gagggaagta tccaagcatt gatcaacgat gcttatcagc
    26641 aagcaaacag ctttttacga ggaaaagatt atctaaaatg gctgccaaag aaagaaaaac
    26701 atacgaaaca aaaaaacaaa gaagatatcc atgcgcatta tgacttaggt aacgactttt
    26761 ataaactttg gctagatcca actttgactt attcttgtgc ttatttcaat acacctgaag
    26821 atacgttgga acaagcacaa atcaataaag tccatcatat cttagacaaa ctctttatca
    26881 aagaaggcga aacgttgctt gatatcggtt gtggctgggg aacattgatg ttcactgcta
    26941 ccaaagagta taatgtcaag gcaactggga tcacactaag tgaagaacaa tacgatttga
    27001 ttaccaaaag aataaaagaa gaacatttag aagataaatg ccgtgtatta ttaatggatt
    27061 accgcgaact aaaaggcgaa acgtttgatc atatcacaag tgtaggtatg ttcgaacatg
    27121 taggttctga aaatcttgaa ggttatttca aagtggtaaa agatttattg aaaccaaaag
    27181 gaacagcttt gatccatgga atcagccgac aacaaggcgg tgcgacaaat gcttggatca
    27241 ataaatatat tttccctggc ggttatgtcc ctggtgttac tgaattagtt gataatatta
    27301 caaaaaacga tttgcaactg attgatttgg aaagcttacg tcgtgactac caattgacat
    27361 tagaacattg gacaaagaat tttcatcaag tccaagatca agtcacgaaa gaaatgggtg
    27421 attccttcta tcgaatgtgg gatctatatt tacaagcttg tgcagcttca tttgaatcaa
    27481 gtaatatcga tgtgattcaa tatttgcttg ttcaacctgg aaacaatgac atcccaatgc
    27541 atcgtgcata attgaggcaa aagtactgtt taaagactat ttaaaaagaa gctaagtttt
    27601 atgatgctcc agaagtgaac ttttttgtct aacttctagg attgtctcta aagcttagtt
    27661 tctttttctt tatattttca taataaaagt ttggttcttt aatttttaaa catgacagaa
    27721 aattttttct tcgttatact tgtaatagaa tactatttta taacaaaggg gatcgaatca
    27781 atgaaaaaaa ctgttattta tactagttta tttgcagctt tgtttttagc tgcttgtcaa
    27841 tcaaatacca atcaaggcac atctgggaat acgtcttcca gtagcgaaca gcgctcttca
    27901 caggtatcgg actcctcaac aaaagcaagc acatcttcgt ctagccagac aaaaagttct
    27961 agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag ctcgtgcgat cgaattgttc
    28021 caagataaat acccagatac tgcaattact agtttggaat tagatagtga ttggggatct
    28081 tacttctaca agatagaagg tgtcgatgat caaaaagaat acgaagcaag aattgatgct
    28141 caaaatgaaa aaatggagac agagaatcca gaaactctag atcgagatga acaaaatggt
    28201 caaaagaaaa aagaagatgg tctggatgtt gataaattga tcacgattga ggaagcagca
    28261 aaaatcgccg tcgacaaagt aggaagcggt acggcaacag attgggattt ggataaagat
    28321 ttaggtacta cttattggga agtcaaagta aaagacggca atcaaagtac atcagtcaag
    28381 atcaatagcc aaacaggcga agtactgtca actgaacaag atgattaaga atttcttcgg
    28441 aaaacaagag ttgatttttc ttttagtcat gatacactag acaaaataac ttgtttgaag
    28501 gagcgattta cttgagttta gaaacgacct ttgccaatca tatctttgcc aatcctttga
    28561 tgaatgcatc tggtgtccac tgcatgacta cccaggaatt agatgaactg gctcattcag
    28621 aagcaggagc atttatcact aagagctgta cgatcaatga acgaaaagga aatcccgaac
    28681 cacgctattt tgatgttcct ttagggagta tcaattctat gggacttcca aacttaggtt
    28741 tttcttatta tctagagtat gctcttgcgt atgaaaaagt gcaggaaaac caaaatcaac
    28801 cgttattttt ttccattgcc gggatgagtg tccaggaaaa cttggaaatg cttgagaaaa
    28861 tagaaaaaag tgggtttaat gggatcactg aattaaattt atcttgtccc aatgtgcctg
    28921 gaaaaccaca actagcttac gattttgagg ccacttatga aacactgaaa gaagtattca
    28981 gcatcttttc taagccgcta ggaatcaaat tgcctcctta ttttgatttt gctcattttg
    29041 atcagatggc tgatatcttg aatcagtttc cattgaccta tgtcaatgct atcaacagtg
    29101 tgggcaatgg tttgtatatc gatacagaac aagaagcagt agtcatcaaa ccaaaagaag
    29161 ggttcggcgg cattggcgga gagtatatca agccgactgc tttggcgaat gttcgcgctt
    29221 tttatacacg tttgaaacca gaaatccaaa tcattggtac aggtgggatt cggacaggac
    29281 aagatgcatt tgaacatctg ctatgtggcg caagcatgtt acagattggt accgagcttc
    29341 ataaagaagg gccggaaatt ttttctagaa tcataaaaga attaacgcag attatgtcag
    29401 aaaaaggata tacgtcaatt gacgaattta aaggcaaatt acgcaccatt tcatagcaaa
    29461 gagacgaagg tcatgattga tcttcgtctt tttttataaa aaacactcgt ctgttcattc
    29521 ctgtgcaagc gtactcattg tatgttagac gaacttgtgg gaaaataaca gaggcaaaaa
    29581 ggaggataat atgaatgatg caacagaatt agcaaaagag cttaaagaaa aagaatttag
    29641 ttttcaagaa ctagttacgg atatacagaa aaaaataaag aagcaaaatc atgaattgaa
    29701 tgcgtttgtc gcgcttgaac cattcgagag taattcccac gagctattga ttccccagtc
    29761 agaaaaaggg ccattggctg gtatcccatt tcctttaaaa atgctcggtc aagaaaaaaa
    29821 gggatggcta gctacttcag gatctcgttt atttgaaaca caccgagctt ctcatacatc
    29881 taattatgtg cggcaagcag aagcgatagg tctagttcct tttggacaaa ccaatgcgcc
    29941 agagtttggt ttcaaaaata ttacagaccc agtcatctat ggcccagcaa gaaacccttg
    30001 gaaccttgaa tgtacaccgg gcggatcaag tggaggagct gctgctgcag tttcatcagg
    30061 aatcgttcct ttagctggtg ctagtgatgg cggtggttct attagaatac ccgcttcgtt
    30121 ttgcggattg attgggctga aaccttcgcg agggacgatg ccagttggtc catatgcttg
    30181 gagaggatgg caaggagctg ctattgattt cggcttgaca gtttctatga gagacacgga
    30241 ggctttattt tatgggatga gaagtatcca ttccggtgcg ccatatcaag tatcgccagt
    30301 tgagtggcaa acacatccaa gaaaaaaacg gttgaagata gctttttgtg ttgattcacc
    30361 gatccactca cagattagtg aagaggcagt acttgctgta aaaaaagctg tagatttcct
    30421 tggaaaagaa gggcatgatt tgacagagat tccttaccca ctagatggtc gtaaactgat
    30481 cacgtcttat tatcagatga atggcgcaga aacagcagca atgatccgct cgattgaaac
    30541 aggcatccag cgagctgtca gaaaagaaga acttgaaccc atttcttgga ctttgttcca
    30601 atatggtcag aagctctctg ccgctactta catcgattct ctgcatgtat gggatgaagc
    30661 agcaattgtc atggaaaacc tacatcaaga atacgatcta ttcctttcac caacgactgc
    30721 atttaccgcc cctaaaatca gcgaagattt gcaaagtgat gcgataagag cacagatgga
    30781 acacatcagt gattgttctg aagtagaagg tttagacgtt gtttcttcca tgtttgagga
    30841 gagcttgaag atcacacctt atacacagtt ggctaatctg actggacaac cagcaatcag
    30901 cttgcctact catttgacaa aagaaaacat gcctctagga atccaattta tggctgccag
    30961 aggaagagaa gatttgctat ttcaaatcgg ctatctattt gaagcaaatc atcagttcca
    31021 tttaccaaat ccatggaaat agcagaaaca aggagaagaa gaatgaagaa aaaaagattg
    31081 tttgctctaa taactagtgc cttttttatt ttaagcgctt gcggtacagc tgccgctaat
    31141 caggaagata acagctggag taatgtagaa aaaaagaaag aattagtagt agcgacttct
    31201 ggcacactgt ttcccgcatc ttattataat gatcaaaatg aattagttgg ttatgatgta
    31261 gatttggcaa aagaggtggc taaacggtta aatgtcaaaa tctcctttaa agaatacaac
    31321 gtggatggac aaatcacttc tgtttcaaaa agagaatcag attttgctgc gaacgatttt
    31381 ggtatcacaa aagaaagaga aaaaaaattc tccctttcag agactttcaa atattcattt
    31441 gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc actcacttga agatcttaaa
    31501 gggaaaaaag cagctggcga acccaatact agctatatgc gtttagcgga aaaacacgga
    31561 gctaaattag tcacatatga caatgccacg aatgatcagt atttaagcga tgtagcaaat
    31621 ggtcgaacag atgttattct aaatgattat tatctgcaga aaatggccgt atctgcttta
    31681 ccagatatcc ctgtaaaaat actggagaac gtttatttca atgaaaacga aagtggtttt
    31741 ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg atggcgtatt aaaagagatg
    31801 aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt ttcaaactga tgtttcagtc
    31861 aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat gtactttcct ctaattgatt
    31921 tttccctcat gtggacttca cttcctttcg tactgcaagg cttggtatac acattaggga
    31981 ttggcttcgt gtcgtttctt ttaggaaatc tggtagggtt gcttttgaca gttttaggtc
    32041 ttttggattg gctgcctctg aatgtattta ttcgttttta tttatcgttc tttagaggca
    32101 ttcccgcact agtcctgctg tttctgttat attttgggtt accttatcaa ttaagtgcct
    32161 taacggcttc agtcatctgc tttacgatta ccagcagtgc atttattggt gaaatctatc
    32221 gaggatcact agcaggcgtt tcttcaggtc agtgggacgc tggtttagct tctggttttt
    32281 ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc ttttcgaatc tcagtaccgg
    32341 cactcagtaa cgtagctatg gatctagtga aaggtacttc gttagcagca acaatcacag
    32401 tgccagaaat atttcaaaaa gcaaagatca taggcggtcg gacattcgat tatttttctc
    32461 tctatatttt agttgcactg atttattggg tcatttgtat cgggatcgaa aaagtgcaac
    32521 agtatttaga aaaacgttct atgcatcagt caatctgaaa aatagaaatg gtgctagcgt
    32581 ggctggcacc atttctattt tttgtttctt gtatattctg cctagtattt taaaaccatt
    32641 tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa aacatgaaga ccccgctaac
    32701 catcgaaata gaatcctcgt tataccaata ttgcatcgag caaggcggct tggttgtaga
    32761 agaagtcacg atgcctgatg accaaggaat tgtagatacg cttagctgct tgacaaaatt
    32821 tgatggaagc cgagaatggc gatgctatga gctgaaagtg acaaaagcag attttcgctc
    32881 tacagccaaa ctgtcatttg ttggacatta taattatttt gttttaccaa gtaaactata
    32941 tgaagaagta aaaactgaaa tccctacagg aataggtgtt ttagtgtatc gtccgtacac
    33001 ggttgaagag gagatgcctg cctctggtac atttctgatt gcgaaaaaag cagcaaagaa
    33061 agagcttgct gtaccggaag aagctttgac aaaccgcttc atggcttctt tattcaggga
    33121 agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat tttccaacag atcaattgta
    33181 caaagaattg aaaagacgtt cagaaaatca agatccattt tctaacgaac gatattatga
    33241 acggttttta gaggaaatag ctagtcaggc aatcaatgag ttgcaagaag aagttatcgc
    33301 gcttcagcat gattatgaat atttgaggca acaacagcag atccgtcggt tgcctacaga
    33361 gccattggag tgaaaaatat gtactatcct tatttaagag gaaaacaatt tgacctatta
    33421 gcattaaaag aagcactgtc gcgaggattg ctctccaata aaatccaacc agtgatcgaa
    33481 ccagtacgtg attccgctac gcttaaaaat gtgatcgaat tatttcaaaa aaagcaccat
    33541 cgaatcgctg tgatccaaaa tccccaagta ggacaattca aattattcga tcagcatgtg
    33601 cacagttgga cagtcaaaga aaatagctct gtcgtccctg cacagatctt aacgccagaa
    33661 aatctcagtg aagtactgga ttcacctcca gcgtttcttg ttttcgatgg acagcactac
    33721 ccaaaagata cagaggtgtg gaaacagttg gcaggagttg attcgaaatt tttgatacca
    33781 gatactagtc gctttcgcat ctggctgccc gaaaacaaaa ttgtgatccg tgactctttt
    33841 caaacgagaa agcatgttga aagttatgca gataaaacag atgacttttt tagtgatgac
    33901 tatctttttt tccacgcaga cggatatatt ggattttctg atttcacgat cgaaggtagc
    33961 cgttatttcg ataaagggtt ccctagccga gcgcttgcta tccatctaac gtatattgat
    34021 gcttacggca atatacgcgt gaaacatttt gtctctgaca gtaacgactc tgctaaagac
    34081 caagcattga agtttcttga agcaggagat aaaatgaaaa aatggatcat gcgtcaccat
    34141 catcaactct tgattacttc aggaatgatt gaattattgg ctctttacca gcaacaaaaa
    34201 ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc atcatttgga acttattagt
    34261 cacctgctag atcatccaaa agattggctt aaatattatt caaaagttca ggaactatat
    34321 gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa tagaaaagag gaaactggat
    34381 gtatattcgg caaacaagga aagaagaagt agaagaagtg gtagaaatca ttaatgccgc
    34441 agcggatttc attgcttcgc aaggctctcc tcaatggcaa aacgggcaga aaccaacaaa
    34501 agaaacgatt ttgaacgata ttgaaagaaa agaaagctat gtattaatag tggaagatca
    34561 aattgctgga acagctgcat tagtctcaag cgtggatccc gtttacacaa aaatagatgg
    34621 tagttggaaa gaatcaactg gtccttattt atcgattcat cgtgtcgctt tagcagaaag
    34681 tactcgagga aaaaaactag caaaaccgtt cttagaacat ttgttgacga ttgcagccag
    34741 caaaaatatc caagatatcc gtatcgatac ttatccagct aatgaaccga tggaaaagac
    34801 catctattct gctggattca cttatcaagg gatgattgaa tttccttttg aacacggcga
    34861 aagaaaagca tatcaaataa tcatcaattg actataaaat catctgagca aagataaaga
    34921 cattaacact actgaaaaaa acaatcatat gatagccaag aggttgtaac agaagtggcc
    34981 agcttcaaaa aacaagaaag aatttccgaa aatgcttctc aaatttttgt ggaattttag
    35041 cgtattttac gaaatagctg ctttctgaaa ctcccttcat cgagaggtcg tgacatagct
    35101 tttgtcacga cctctcggct tatcctacga gtattattcg atacatttat atcaataatc
    35161 ctttttttat tttatctgca gcttcttgga acgctccatc gtttgacacg actaaaagtg
    35221 aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc tacataaacc gccagtctgg
    35281 ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg cgattgcaat gtttctttag
    35341 agatcgtaat aaagatagga cggactaccg tcccgaaata ctcatataaa tgccaaaaac
    35401 catttgctgt caaaacggcg taacaatttc ctactgtcag tttcttttta atttctgctt
    35461 tactggaacc atatttatgt tgagcatatt ggtcccactc tgcaaactca tttcccttga
    35521 tcatctgatt gaatgtattg tcagaaacaa aataataatc ctcttgatct ttttccccag
    35581 gtcttggtga tcgattcgtg taagtaatga tcttttgttc cggctgaaaa atagcttctg
    35641 cgagtgaagt ttttccagaa ccagaaggac cgataaatac atagcaataa ttcattgtca
    35701 tttctccttt gatgattgaa caagtgaaaa agctttggaa aatatttcca aagcttttaa
    35761 aagtaacttt gcttttttgt atattatacc agcaagccag tcacaatagc acttaaaaag
    35821 ctgactaaag tcgctgttag caaaattttc aatccgtgtt ttgcgacaag atttcctttt
    35881 tcttcattta atcccttcat cgcaccagaa atgatcccga tagaagagaa gttggcaaat
    35941 gaaacgagga agacagatat aattgctgtt gtacgctcag taaaatggaa attaccattt
    36001 gctaattctg tcatagcaac aaattcattt gtaaccagtt tagtcgccat gatactaccg
    36061 gcatccactg cttctttcca tgggattccg ctgatgaagg caagaggggc aaagatgtaa
    36121 cctaggatct cttggaaagt gatgccgaat atcccgtgga agatagcatt gatcatggca
    36181 atcaaagcaa caaaaccgat aagcatagca gcaaccgtga tcgccacatg gaaaccatca
    36241 agaatgtatt ctcccaacat ttggaaaaat gtctgttctt tgttttcttc aatgatcagc
    36301 tcatcttctt tttcttctaa tacataggga tttacaattg aagccagaat ataaccgcca
    36361 aataagttca aaacaagggc agtgatcaca tatttagaat cgatcagtgc catataagat
    36421 ccgacgatcg acatagaaac tgtagacata gccgatacac tcatggtgaa caaacgtttc
    36481 tcagatagga agggtaattc cttttttatg gaaataaaaa cttccgattg tcctaaaata
    36541 gcagaagcta ctccattata agattccaat ttgcccatcc cgttgatttt acttaatcct
    36601 agaccaacag ctttcataaa taatggaaga atcttgatat agcgtagaat accgataata
    36661 gcagaaataa atacaattgg catcaatacg gaaagaaaga aaggtgcact tcctttattt
    36721 gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa aagcaagaag atgatcaaac
    36781 atttttgcca atacagaaac aaccgctgta ccaaaagttg tacgtaataa tacgaaacca
    36841 agaacaagct gtaaaacgaa catcacaatt agtggtttca atttgattgc ttttttgtta
    36901 ctgctgatca aaaaagcaag ccctaatgtg acaaataaac caataatacc taataaataa
    36961 ctcaaataat cactcctatt ccaaattccg ttttatttta cctgttttgc aaataaaata
    37021 ctaatcttta gtgaaatttt cattactttt ttagagtgaa acgcattcat tttccattag
    37081 aaaataaagc gaacaaaata aaaggtttaa aaagtgtaac gtcttagtaa agtctataaa
    37141 aaaacgaaac tatcgattaa gctaatagtt tcgttttcaa catttataag taaacttttt
    37201 ctattggtaa tatagatttt cggaagggta gacaggtgcg caggtaatat cgattcctag
    37261 ttttttcaat gtttgttcat catttttatt gaccatcaca gtagagtggg cttgtgctcc
    37321 ctcaagttca gctagtttat cataagcaac ttgagcgatt ggattcgtta ctgcactgat
    37381 tgataaagcg atcaagactt cattgatgct tagtgcatcc aaagaggaat gaagtgtctt
    37441 tgatttcaat ccttgtattg tttcaagaac taatggtgac aataaaggaa tgtcatctgc
    37501 gatatgggct aaatgtttta ctgcatttaa gataactgcc gctgacgcat ccatcaatga
    37561 acttgtacgc ccggtaatga tgacttcatc tggtaattcg attgcaatca cagcagaagg
    37621 atcttttgta ggactttgcg cttgaatctt atctgcatag tcatgggcag ggactaccac
    37681 gtttcggtct tcaggacgta aaccgacttc ttccataatc agtttgattc gattaactgc
    37741 ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt tggaaataac gacggatgat
    37801 ttcttgttta gaggcttctt ggacaacggt atcatccacg atcccaaatc ctacacgatt
    37861 cacacccatg tcagtaggag attgataaac agattcttta ccagtaattt tttcaataat
    37921 acgtttgatt acaggaaatg tctcaacatc ccgattatag ttcacagcta ctttattgta
    37981 ggcatcaaag tggaaggaat cgatcatatt gacatctttt agatcgactg tggctgcttc
    38041 ataagcaatg ttcagcggat gtttcaacgg aacattccat actgggaaag tttcgaactt
    38101 ggagtaacca gctgcatgtc ctttttggct ttcatgatac agttggttca aggaagtagc
    38161 gagctttcca cttcctgggc caggagctgt cactacaacg attggtttag ttgtttcaat
    38221 atactcattt tttccaaaac catcttctga gacgattttt tctacgttga ctggatagtc
    38281 ttcgatttct gagtgtttat agaccttgat gtttcgtctt tctagtttat tgataaagac
    38341 tttcgtggca ggctgtccac tatagcgagt gataacgaca ctgttgatta ataagccata
    38401 accacgtaat tcatcgatta accgcaagat atccatatca taagtaatcc cataatcacc
    38461 acggatctta ttacgttcaa tatctccagc atagacgcaa atcagtattt cagcttgatc
    38521 tttcaatttt tgcagcaatt tgattttcgc atcttcttca aatcctggaa gtacacgttt
    38581 cgcatgcttg tcgtcaacta gcttcccacc aaattctaaa tataatttgt catagtggtt
    38641 cactcgttcc aaaatatacg ctgactgctc ttcaatatat ttttctgaat caaaaccaat
    38701 ttttttcact aggttccctc cttaagtact aaagcatttt ttcatttttt tgaggattcg
    38761 tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac caacttgtct
    38821 agatagttat tcattggtat gatagttttt gataaggaag gtgctacatg aaaaaaagac
    38881 agaaaaagaa aaacgcatat aaacactata tccgttcgat ttttaccggt tatgaaagaa
    38941 tgcttgaaga tcccgaattg gaacaattga cgtttactta tctaaacgaa gaaacgcagc
    39001 taactcgtga tgagcatcag cgcattcatt tcacaacaag agatctgcct agtaaataaa
    39061 aaataagata aaggtcatcg gattcgattc tgtcctatca gatggaaaga ctagttgatc
    39121 gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta tggagcattg cccgctcttt
    39181 tttataacaa ttttttacag aaaatggtac tattgaagta ttaataagtg aaaaacagat
    39241 gagtaagagg aggcaagtag tatgaaagag ccagatacaa atgcaaatta tgagccagac
    39301 gaactagagg agacgaatgc ggatcaggat acaggacaag aatcgtcttc tttttacgat
    39361 atcaaagaaa aagcaagcat gaatctttcc caattattag aagacttaca ggcttttgaa
    39421 aaagctgtcc gcgaagaaga tatccctgag atctaccgtt tatacaatgg ccgattgaat
    39481 gaagaattaa aaagaagctc aaatgaaaat cacgagatcg atcaattgct gatgcgtaag
    39541 atccatgatc gttttttaca ggaatttcct tttatggaac aagtagaaaa catctcgcca
    39601 acgatgagct attacaaaat cggcacttat taccatgatc ggcccacggt cggtattgat
    39661 gcaagtctgc cagaaatttt tgttttgcct aagatcgatg aagagtggga gaaatattcc
    39721 caaccggagt caacggacta tgacaaaaaa atcaatgagc tagacgcaaa ggtgatcact
    39781 gctcagacag aaattgaacg tttagatgaa caaataaaag aaatcaatcg acaaatgact
    39841 gatttgaacg acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga aattcaagaa
    39901 ttagagaaga agaagcaagt actatctaat gaaaaattag gctggcttcc ttatattgag
    39961 acgcctgaaa caatccagca gcaaaaagaa gcattgaagc aagaagcaag agcagaccag
    40021 cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca gacgctattt cggtagcaaa
    40081 gagggattcg gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa tgagaatcct
    40141 aataatcaat ctaatgaagg aggttccgag tatgaatgaa cacgatgaag cagaatggac
    40201 aaaaaatgaa gaacaaacag aagatcatga agaaacgaac acttcattgg aagaacaaac
    40261 aagtaatcct ctggatgaca cagaagaaat ccctatgcta gaggaaacaa aaatacacga
    40321 aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa aacacccatg cttacgaaat
    40381 aactgaaaat cccgggtatt tagaaggaaa agagcaagat catcaagata tcgtcagttt
    40441 gattgaaaca gcggaagcag aattagcgga tttacgtatg agaaaagatt tgatcgaatc
    40501 agattttcca caattattag aatcatatga tcgttttata ttgaatgaag aaccaatctc
    40561 cacgatcgga aagacaagcc tgatcgaata cttcacgtat gaattactga aaccgttgaa
    40621 tgatattggt ctggaacggt caaattcagc ttatgacaca tggaaaacaa gacatttttc
    40681 gatcaattat acgttgaatg gacaaaacga attagtcttt tcctttgtat taccgaccat
    40741 cgatcaacga taccaagtgg aatcgatgag tctcctaaac gtatcaccag agacaatgga
    40801 aatcaatgtc caagatgacc gggtgctttc tttgatccga cactggtcag tggatcgagt
    40861 attctctgca gggcagatca cgatattcaa tcacaaattg aatcaattat tagcccatgc
    40921 aagaacactt ggttttaccg ttaatcagac attactagac aatacgaagc cacttcgttt
    40981 gaatcttcag agcgaatttg aattgacgaa tgaagtattg gatgatattt ttatcgtggc
    41041 aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa actacttatc aagtacgatt
    41101 agataaagga cagtctttaa cgataacaac aaacgaaaaa gatcaaacag tcttgtcgat
    41161 ctcatctgga gactacccac gatctgtcat tgatttcttt atcaattacg aattcttggt
    41221 cccattaatg gtgagaaagt cctgattgga taagttggat taccagatgt cattcagata
    41281 attctgttcc aaaggacttg cggcagttat ccagaataat tgtcacaagt cttttctgta
    41341 tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag gaatgtccac
    41401 aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta ggatcgcttt
    41461 tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag tactttactt
    41521 aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca gtttttattt
    41581 tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc tgagtttgtt
    41641 ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg gagtctgttt
    41701 ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc tgttatatcg
    41761 tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga tgcacagtat
    41821 aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt acaatcattc
    41881 aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc agtatactca
    41941 ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag gaggaagtaa
    42001 aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt tcggaaaaag
    42061 aagtgttctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat tttgaagtca
    42121 aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag aaaaatcaac
    42181 tctattggat gtttactgtg gacgggaaaa gtgctactac tggtgccaaa gagatcaaat
    42241 tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca taaaaaactg
    42301 ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag gaaaataaca
    42361 tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg gctaatattc
    42421 tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc atgctaaacg
    42481 aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc ggtgataaac
    42541 aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca aaatactttt
    42601 tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg gtgatggaag
    42661 cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt gttatagcaa
    42721 tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg aaagaaatca
    42781 aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt cgacacagtg
    42841 aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc ttggctttag
    42901 caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc agcggattgc
    42961 tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt tatagtctga
    43021 tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct tttgaacgtc
    43081 ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg gagattttag
    43141 ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg gtcatcaatg
    43201 atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa gatgtatata tacattgaaa
    43261 cagataaata tgacccaaat tattctcgta aataaaacgt ttattcatga attgaagcaa
    43321 aaatgagaaa aatataatct tgtttttaaa taaaacggct acaatttagc gaatttatgt
    43381 tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga tattttaatg aacaaaaaga
    43441 aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc actttgtata agctatatac
    43501 cagtaggatt agcttgcgga gtattgttgc aaaaagttgg atttgatcct ctcttatcag
    43561 gactgttatc gattctggtc ttttccggtg gcgcgcagtt tttagttgca tctatgctta
    43621 cgacgcaagc atcatttgca acgacattat tgatggtttt ctttttagaa ttacgctata
    43681 cattattagg agcaagtctt gccggtttta tgaaaaaaga aaagcggacg tttttggctg
    43741 tcttttctca atcactgaat gatgaaaact atgcggtgaa ctacttaaag ttttcaactg
    43801 acccttcctg gaataaaaga aaggctttat atgtcaattg gttctcaatg actgcatggg
    43861 ccggtagcaa tatgatcggt aattttttcg gctcggtgat tcgagtagat gctgaccttg
    43921 tccattttgc tttgacggcc atgttcattt tcatgtttat catgcagatg aaaagcgcac
    43981 tactgatctt tacaggttta ttctcaggcg tattaggggt cgtattcatg gtattattcc
    44041 aaaatacttt cggattgatt gcagcaacag tcattgcttc atttgccgga tttatgctgg
    44101 agaaggcatt caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa gatgtcaatg
    44161 agccgctctt caacttcccg gaacaggagg ccaatcacca tgactaaact atatttgcta
    44221 cttttagttg cttgtctgtt catcgtggca tatatcccgc gcttgttccc gatgctttat
    44281 tttactcatc gaaaagtgcc atcctggttc agtgactgga tgaaatatgt accggtggca
    44341 ttatttgcgg cgttagcttt taaagatgtt ttcattactc atgaacatct ggatattgct
    44401 tggaatatca aaatagctgc aatgattcta gtagcaggcg tggcttacaa aacacgttcg
    44461 atggcgttgt cagttttgac aggacttgcg tctgtctttt tgctgtctat gctttaaact
    44521 gttttatttt attaaaaaca gagattgcga cataagttgt tcaaactgat ctatccgaac
    44581 tgcatttgca acagtcaatt ctggatgcaa gtttattcgt agggataaaa aagattagtg
    44641 ttggcttatg atgcaagctc ttttttattt taggaattag agagggattt gtccttctat
    44701 gattttttgc tataataggg aagatgttta ttttatgaaa gaatagttga gggaatttaa
    44761 atgaataaaa aattgatgca aggaacattt tggctaacct ttgctaatct tctatgtaaa
    44821 gttttagggg tagtctattt gattccctgg ctaagtatga tggggaacaa tcaagatggt
    44881 atgcttgcta cgacattgta taatgttggc tatcttcctt atggattgtt cctgatgctt
    44941 ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag ctgtagccac aaaagaagga
    45001 gataacgctg cttgtcggct gatttttaga agtacgatca atatcatgtt tgtcatcggt
    45061 atcgcctctg ctgcactgat gtatttattt gctcctttac tagccggaat cagtccgatt
    45121 gctaacgtta ataatgggat actagctatc agaagtttat gcccatcttt gattgctatc
    45181 ccaattctta gtgcaatgag aggatatttc caaggaaaaa atgatctgcg gccttatgga
    45241 acttctttga ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg tacttattac
    45301 ttgcgcgtat tgactgacgg aaccatatta gaagctgtat tgatcagtac agtcgcttct
    45361 ttcttcggtg ggttagctgc gattatccat atgtatatcg tcggacgcag aaaagacttc
    45421 ttcgaactta aagatttttt gatttccagc cgttatttcg aaagagaaaa ccgatctgcc
    45481 tctgtctcta tcattcggga aacactaccg tttatttttg tcggctcagt cattactatc
    45541 gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc actttttccg tccagagatc
    45601 gcagatcagc aactggaatt cttattcagc cgtgcttctg tcaatccaaa taaattgaca
    45661 ttgatcctga tctccatggt aggaacagta gctataagca gcttgccaat attaagcacg
    45721 atgaagaaaa aagatcggat acaaatcgaa aaaacagtag gagatagttt ttcgattgcg
    45781 ttattgattc ttcttccttc attgactgga atgtctttat tagctggacc actatacaca
    45841 ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt gctagcttct
    45901 ttgtttttct ctttattcac gatcctatct acgatgcttc aatctttgaa tcaccatcgt
    45961 tttgcgatcc gattaactgt agaagcaatt atcctgaaag tcatcttcca agtaatcgga
    46021 ttaggattag ttggcggata cgggatgagc ttatcaaatg gcttagcatt cggtattgtc
    46081 tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa tagcaccttt agcaaagatc
    46141 agcaattttt tcttaaaaac tttccgctcc accctgatta tgcttgcagt atgttttgcc
    46201 gttttctttt tgctgaatca gcgattgtca atggaatcaa aaagctatgc aatgatttat
    46261 tgtgtggctg taggaagtat tggcggctta gtatttttat tttctcaatt cggaaaaaat
    46321 gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa gcaaagaata ggttaactaa
    46381 tcgttttttg ctgtcacttg aaaaacggca actcgtatga ttacgcttgt cgtttttcta
    46441 ttttccaatt ttagtttcat atctaatctt acttatgata tagttataag gtctggaaaa
    46501 attctttgaa acgaggaaat taaacacatg aaacttgcag tagttacaga tagttcggca
    46561 tatctgccgg aacgtatcaa gaaccatccc gacttattcg ttattccaat ccctttgatt
    46621 atggatggga aaatttataa cgaaggaatc gatatcgaag cggatgaata ttatggactt
    46681 ttaaaaaaca gtaaagattt tcctactact tcacaacctg tcgtagggga agttttgggt
    46741 ttatatgagg aattgaaaga gaaagggtat gacacgattt taagtatcca tctttcttca
    46801 gggatttctg gctttgtcaa cacattattt gcgatgaaag atgacatcag cggagtaacg
    46861 gtcattcctt atgactctaa gatcactagt atgccgatgg gacacatggt agaagctgcc
    46921 ttagacttaa atgatgaagg tcagtccctt gatgaaatac ttgcacatat cgatcgaatc
    46981 cgagataata cctacgctta tctgattgta gatgatttga acaatttagt acgaggagga
    47041 cgtttgacaa atggtgcagc gctgattggc agcttattaa aaatcaaacc gatcctgaca
    47101 ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa agcatttgct
    47161 cgagctgaag aaattattgg tagaagaaat aacgaaatac aaccgccagt caaactttac
    47221 gtgatacatg caaataattt agaagtagcg attgcggaga aagagaaatt gcagaaaaaa
    47281 tatccaaatg cagaaatcga aattggtcat ttcggaccag tcattggtac acatttaggt
    47341 gaaaaagcca tcggcattgc catttcagcg caataattcc ttttattcaa aaaacatacc
    47401 aaaaatattc caacatcatc ttggactttc tacatgttac taatgagcgt gtaaaagaac
    47461 gagatggtgt ttttatttct atcaatgaag ctctttttct ttgcaatcct cacttcaatc
    47521 ctctataatg tcgcatatag tttagtaaat gagtaaacta ctaatttact tatttactaa
    47581 aaataaaagg atgaggagtt aaaataatgg aaataccaac aaatttaaaa cataaaccag
    47641 taatcgttgt agaagactat gataaagttg atggaagaaa tgcattaaat acggatgcaa
    47701 aagggctttc actgggcctt gctcagtgga atgatcgtgg aaaagtcgat atctcaggca
    47761 aggtatggcg acatactgga gaaaaatggt cacgacagtc ggaagaatta ccaatcacac
    47821 gtattttaga tttagcgatc ttagctgcac aaggaagttt atacttccaa gatgcttatc
    47881 gttatgaaaa attttatgat ccagaaaatc cagtcgtaga catcattgga ttacaaggga
    47941 accgaatgac ggtagaagta gataccaaaa atccaaagat cgatgaagac atcattttgt
    48001 attacgatac actacaaaaa gacggagaac tgattgggga acgattccgt attttaaaac
    48061 gtcttttaga tgagctagga tattaataga cagagatgat caaaagggga agagaagcat
    48121 ggagaatttc tcactaacag aaaaccgaac cttttcacgg ctagaaaagc aaatgatctg
    48181 gaagaaaccg aaaagccatc ggacaagtgc agaagaatgg cggatcgcct ccgaaatcaa
    48241 agggaattgg caagatcctg aaatgaaaat cagtaacgta cttttggaag gagatgccgg
    48301 gtcggggaag acacaattag ccaaagcact atctgctgat ctgcaacttc cctatacaaa
    48361 agtgacctgt tttgcagata tggacaaatc agatgttttc ggagccttat tacctgtagt
    48421 agattctaat aaggaacaag accaagaatt actagaagct atttaccaaa cagatacact
    48481 tgaagctgta ctgacactga tccagaacca ttacggcatt gatccttttt ctgctaaaga
    48541 aaaattagca gagctagttc aaaggatcga agctgattat ccagatgcaa tccattacag
    48601 ctattaccct tctgaaatcg ttcgagcgat tgaaaaagga tatttattag agattcaaga
    48661 gccgactgtc atccgagatg cctctgttct tgtggcactt aactcagcgc tcgaaccaaa
    48721 tgggatgttg aatttaccga caggaatcat tcgtcgccat ccagattgcg tcattgtgat
    48781 cactacaaat cgaaattacc aaggaaatcg cccattaaat gaatctttaa gagatcggat
    48841 gcaacatgcg gaaaaaatgg atcttcctgc cttagaagtc atggcagaac gtgcgatggc
    48901 gaaaactatg atccaaaaac aagaacttct cttaaagatg gcagagatta tccgtttgtt
    48961 ggatgaaact gcaaaagcta acgccatcaa aggtgtagca ggaatgcgtt cttattttta
    49021 ttgggcaaat acctataagc aaggacaaga tttactccag tcgatttatc caaaagtttt
    49081 gtataaatta tccactgatt cagatgaatt ggcaattctt gaacaagctt tgtctgacag
    49141 tggattgcta ctggaacttc aggacatatt gagaaaagaa aaatggggtc atcctcaatc
    49201 tgagcataca aaaggaagaa ccattagcgc cgaagaagca aatgaacgaa acatcactga
    49261 aaatgctgaa gaaattctgc gcttagatga aaagaaatcg ttgtctgctg aagaaccaaa
    49321 tgagggagaa gagaaacaag aagaaacaat agaaaaaaac gagattcctc ctcaaatgca
    49381 aaaagaacgc agtagcgata tcgaaggaaa aaatcaaaaa gaacaacaaa caggagatga
    49441 ttctcaagaa atgtctgctt ctgacatgga agcacatgat aaggaaatca agaagcaact
    49501 gaataaagaa gcgcgacaaa tcatgaagga aacgattcat gaaaaagaag gtctcatcgt
    49561 tcaccgacca gctttccaag gaaaaacgcc ggaagcacgt gagctacaca tgcaagtaat
    49621 gccaatagta gaatcactta gccggcaaat tttagagtta ctggataatg aacaaacaga
    49681 gacgtaccaa aaaggaaaat acgaaggtca gcggttcaat gcaagcaaag tagcatatgg
    49741 tgatttgaga aattttgaca aaaagaaccc gccacatgag caaccttctt tagcggtagc
    49801 tgtacggatc gatgaatctg ggtcaatgat acgggatgat cggatcgaag cggcaaaaaa
    49861 agcagcgatt gccattgccg aattttctaa gaaagtcgat atcccattgt tgatttacgg
    49921 agatacagca gaccgttcgt caagagaaaa gacttctttg ttttcttata aagaatttga
    49981 agacggattt caatggttaa atgaaaaatt agtcactatg aaaccaaggc aaaacaatcg
    50041 tgatggtgct gcacttcgtt tagcagcaga aaaattaaac agacaatccg caacaaccaa
    50101 actgttgctt aatattagtg acggtcagcc aaaagcgttg ccggattata caggcaaaaa
    50161 agcaaaagaa gatatccagt ctgttttgaa agaatatgaa cgtcaaggaa tcctatttgt
    50221 ctccgcagct attggccaag acaaagaaga aatcaagtcg atctacggtg aaagtcgttt
    50281 tgtagatatt actgacttga atgaatttcc caagcaaatg attcaactga ttgcccgcta
    50341 tctatgataa aataaagctc aacgaacgca cttagcagat tcgttgagct tttcgtttct
    50401 agttaccggt aacaaggata cttctaaaat agaatcggtt ataatcaaga gggaccgtta
    50461 aaggagtgaa acgatatgat acaagaacaa aaatggtgga aagaagcagt aggctatcaa
    50521 atctatcctg ctagtttcaa agatagcaat ggcgatggtc aaggagacat caatgggatc
    50581 cgtgaaaaac ttaattattt gaaagatctt ggtatcggct ttatctggat caatccgatc
    50641 tatcaatctc catttgtcga taatggttat gacgtaagtg actatcaaga tatagaagag
    50701 agattcggaa cgatggaaga ttttgattta ttgctaaaag aagcacatga attagggatc
    50761 aaagtgatta tggatcttgt catcaaccat agttcggatc agcatcaatg gtttgaagaa
    50821 tcgaaaaaat ctaaagaaag cccgtatcgc gattattaca tttgggtcga cggggtagat
    50881 ggcaaagaac cgaataattg gacatcgatt ttcggcggtt ctgcttggga gtattctcat
    50941 gaaactggac agtattatct gcatgttttt gcaaaagaac aaccagactt aaattgggaa
    51001 agcgagaaat tgaaagaaga actattcaat atgatccgat ggtggttgga taaagggata
    51061 gatggatttc gtttagatgc gatttctcat gtaaaaaaag atgaatattc agtaaaagcc
    51121 acagagaacc ctttctcacc ctttcaaaat gtctcaggaa tcgaagaaca tttgaccgat
    51181 ttgaaacatg tttttgaaga gtacgatatc atgactgtcg gagaagcaag tggtgtcact
    51241 gcagaagaag gaccacaatg ggtagggaaa gacggttatt tcgatatgat attcgaattt
    51301 gatcatatcc atatctggca gcaagaaaaa gaagggcaat tagatgtcct taaattgaaa
    51361 cttgcattaa gcgcttggca aacttcattg gatggtatcg gatggaacgc tttatatatg
    51421 gaaaaccatg atgttcctcg tgccgtttca gtgtttggag atactcgtcc cgatttttgg
    51481 gctatgtctg caaaagcaat cgctatgatg tatttctttt tgcaaggtac gccatttatt
    51541 tatcaaggac aggaaatcgg aatgacgaat atgccctttg aatcgatcga tcaagttgat
    51601 gcagtagatt cgaaaagact ttataaaagg ctgcttgcgg aaggtaagac aagagaagaa
    51661 gcattggata tcattcgtga aacaacgaga gacaacagca ggacacccat gcaatggact
    51721 tcggaacaat atgctggttt ttctacacat gaaccttggc tgatcactaa tccaaatacc
    51781 aaaacgatca atgtagaaca acaagaatat gaaccagagt cggtcctgca attctataag
    51841 aacatgatcc gcattagaca aaccaataaa ggtctgatat acggttctta taaagaatat
    51901 cttcatgaac atccacaact ttatgtctat gaaagatatt tagaagatga acaatatctt
    51961 attatggtaa acttaacaga atcacttgct gactatgaac ttccaaaaga agcagatcag
    52021 tcctggacat tactcttgtc aaattcctct agcggagaat ttgaagctaa aggcatacta
    52081 gcaccttatg aagctcggtt atataaaaca aacaagtaaa acttcctcgt taaagttcat
    52141 tagttggact ttgcgaggaa gtttttcaga ttgctctgtc aattactcat ttcaataatt
    52201 cgctctagga ccaccgatat atttaggacg agagcgaagg gctgtcacgt gagcttgtcc
    52261 aagggttttc aaacgaggtc tgacaggtac ttggacatgt ttgatatgca taccgattga
    52321 tgtgtcaccg atatcgatac cggcttgtcc aactacgtgt tccacttcga caggatcatt
    52381 gaactggtca aaagcagcaa cagaacaagc acctccagca tgtagtgcag gaacaacaga
    52441 gacaatttcg aaattttttg cttctgccac tgttcgttct actactaatg ctcggttcaa
    52501 atgttcacaa ccttggacag ctagcgcaat ttctttagga tccaacaact cttttaacgt
    52561 acggatgatc cattgaccaa cttccgcact ggaatttttt ccaatatgtc ctccaacgac
    52621 ttcgcttgtg ctgcatccta aaacaaaaag atcacctttt ttcaagtgtg cctcagccaa
    52681 tatgtcatta actatttcag tcaattggtc ttttaattct ttttctgaca acttcatttt
    52741 ctaaaacctt ctttttcaat taatattgct tccgtgcttg aatacgttga agacttgcag
    52801 ttataggaaa ggcaagtccc attccaatca gattttggaa gatgtttcct ggtatagaag
    52861 ccactccagc cggccaatca tataaaatcc aaccggcgat accatagccg atgatcataa
    52921 ctaagcttcc taataaaagc cctaatcccc acatacgagt agaagatttg ctgaaagaac
    52981 cagcaacata tccttgtaac ccgtggatca ctaatgaaaa aactgcccac tgcggatatc
    53041 ctgaaatcaa atcgatcaat agaccactgg aagcaccaac aactaatcca cccgttggac
    53101 cataaagaaa agcagcggta tagatcccgg cctcgcataa tgtcacaaat ccattcgtag
    53161 ccggtacagg gatcaaaatt gttaatgaaa gggcaactgt caacgcaatc agcatagcat
    53221 agatcgttaa ttgcttagtt ttcatttctc attcctcctt tttgaatacc gccagaccag
    53281 acacttccgg tatgatcgtt catcagcacg ccattagaaa tacaatgggc aacaaatttt
    53341 ttactttctg ctactgcttt ggtaaaatct gcccctaaca caagattcgc tgttattgca
    53401 gaagaaaagg cacaaccggc cccgtttact gttgtagtgt cgattttttt atgagaaaat
    53461 acctcataat gatcaattga atagagaaga tccacagctt catctccagc catccttgtg
    53521 ccgcctttga tcacaatgtt ttttgttcct gaagcagtga gttctttagc gagatggatc
    53581 atttctgtta acgaagaggg aagagcttga ccagaaagta aagcagcttc tgccaaatta
    53641 ggcgtaatca tcgtaacaag tggaaaaagt tcttcgatga gctgcgaaat atacaattga
    53701 ttgatcacag cttcagtttc tttgaatgcc aaaacagggt ctaaaatgat tgggatattt
    53761 tgttttgttt ttatgaagtc tcgaacaacc atgattgctt cacttgaatt caataaccca
    53821 attttgatac catctaatga agtcatttta tcaatcgaaa ctaactgtcg tttaagcaat
    53881 tccaaagata tagattccag cataaatcgc tcattaatat ctagtgcccc tatagaagtc
    53941 aaggcgctta gtccgaatag accaaaattc tcaaaagttt ttaaatctgt ttggattcct
    54001 ccaccagcaa atggatcaga accgccaatt gtcaagattc ttttcataac attttcctcc
    54061 atgaataaga gtataacgaa attttcaaaa aagaaaaaca gccaattggc atatttttat
    54121 ctcatccaat ttaaaaaaac aaaaagaaaa gaaagaccaa aatggatctt tctttcctta
    54181 aatagttagg acttttgtga ttttaattct ttctcgattt gaagccgttg cactttcatt
    54241 gtagctgttc gcgggattgc atcaaaatca cggatgatag ggacgttcaa gcgaggaagt
    54301 tcatggactt gctcccacca tcgattccaa tccatttctt tatcaggagc taaagcaata
    54361 atcggttgtg gagaatgatt tttatccctt acaatcacta cttctgccaa aaaatctaac
    54421 gaatctaata aaaagtcttc aatagctaat gtactattga tcgtttcgat caaatccact
    54481 tgccgatctt tcaaaaatag gtggccatgc ttatccataa aaccgtaatc tccgctatcc
    54541 caccaatcgc cataaacatt ttcctggaac cgtgcatctt ccttgtaata agtcaaagcc
    54601 cgtcctttag acagtaattg tatatgacca tctgtgtttt caggtaggac attcccaact
    54661 gaatctgtaa tccgagcgct tgtcatgtct tccagtccaa cgcccatatc tcttgcatca
    54721 cttgttttca aactctctag tgtatgagcc ttaagaatca ttggtccgca ttcactttgg
    54781 ccatatactt gaagaaatac cgcatcctgt gccatcgacg ttcgcaaaaa tgtcttcatt
    54841 gtctggttgt ttatagcatc aaaagtggag tgatagtatc ggatacccga aaaagcttct
    54901 ggatgctcct tagcagtaaa acgccactga acaaagttat tcggatgggt ctctagtgca
    54961 attggccggt gagcttccaa catatgaaca acttttgaac tttgtgcgtt tgctaaaggc
    55021 atcataggaa atcccatgga catcaaagaa gagataccaa tattgaatct ggaatgcact
    55081 ggagagatgt gaaaagcgat taatttcttt tccgaaattt ttgtgaaaac tgttttttgc
    55141 catttcgtcc gccatcccat agaatgagca gaatgacaaa taagtttagg gataccagtt
    55201 gtaccagaag tatgggtcat ataagaaatt tcatttttgt ctaaaaatga ttgagaaaca
    55261 gacaaagcag gttgttccat taaataaaga atagg
//
LOCUS       DACEMO010000002        54503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEMO010000002 DACEMO010000000
VERSION     DACEMO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.002"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(439..2820)
                     /locus_tag="IW728_000053"
     CDS             complement(439..2820)
                     /locus_tag="IW728_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1023442.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2810..3457)
                     /gene="recU"
                     /locus_tag="IW728_000054"
     CDS             complement(2810..3457)
                     /gene="recU"
                     /locus_tag="IW728_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1023443.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3521..4060
                     /locus_tag="IW728_000055"
     CDS             3521..4060
                     /locus_tag="IW728_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1023444.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4150..4569
                     /gene="gpsB"
                     /locus_tag="IW728_000056"
     CDS             4150..4569
                     /gene="gpsB"
                     /locus_tag="IW728_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1023445.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4591..4958
                     /gene="rnpB"
                     /locus_tag="IW728_000057"
     ncRNA           4591..4958
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW728_000057"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5167..6333
                     /locus_tag="IW728_000058"
     CDS             5167..6333
                     /locus_tag="IW728_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1023446.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6379..7875
                     /locus_tag="IW728_000059"
     CDS             6379..7875
                     /locus_tag="IW728_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1023447.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7956..8639
                     /locus_tag="IW728_000060"
     CDS             7956..8639
                     /locus_tag="IW728_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1023448.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9284..10474
                     /locus_tag="IW728_000061"
     CDS             9284..10474
                     /locus_tag="IW728_000061"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1023449.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10576..11529
                     /locus_tag="IW728_000062"
     CDS             10576..11529
                     /locus_tag="IW728_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1023450.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11598..12947
                     /locus_tag="IW728_000063"
     CDS             11598..12947
                     /locus_tag="IW728_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1023451.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13130..13453)
                     /locus_tag="IW728_000064"
     CDS             complement(13130..13453)
                     /locus_tag="IW728_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023452.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13582..14745
                     /locus_tag="IW728_000065"
     CDS             13582..14745
                     /locus_tag="IW728_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023453.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14815..16164
                     /locus_tag="IW728_000066"
     CDS             14815..16164
                     /locus_tag="IW728_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1023454.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16209..16640)
                     /locus_tag="IW728_000067"
     CDS             complement(16209..16640)
                     /locus_tag="IW728_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1023455.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            17028..19670
                     /gene="alaS"
                     /locus_tag="IW728_000068"
     CDS             17028..19670
                     /gene="alaS"
                     /locus_tag="IW728_000068"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1023456.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19870..20466
                     /locus_tag="IW728_000069"
     CDS             19870..20466
                     /locus_tag="IW728_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1023457.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20588..21736
                     /locus_tag="IW728_000070"
     CDS             20588..21736
                     /locus_tag="IW728_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1023458.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21774..22187)
                     /locus_tag="IW728_000071"
     CDS             complement(21774..22187)
                     /locus_tag="IW728_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1023459.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22266..22697
                     /locus_tag="IW728_000072"
     CDS             22266..22697
                     /locus_tag="IW728_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1023460.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22796..23029)
                     /locus_tag="IW728_000073"
     CDS             complement(22796..23029)
                     /locus_tag="IW728_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1023461.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23212..24882
                     /locus_tag="IW728_000074"
     CDS             23212..24882
                     /locus_tag="IW728_000074"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1023462.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            25029..25415
                     /locus_tag="IW728_000075"
     CDS             25029..25415
                     /locus_tag="IW728_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023463.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25402..26100
                     /locus_tag="IW728_000076"
     CDS             25402..26100
                     /locus_tag="IW728_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1023464.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26105..26569
                     /locus_tag="IW728_000077"
     CDS             26105..26569
                     /locus_tag="IW728_000077"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1023465.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26566..27477
                     /locus_tag="IW728_000078"
     CDS             26566..27477
                     /locus_tag="IW728_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1023466.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27712..28251
                     /gene="pyrR"
                     /locus_tag="IW728_000079"
     CDS             27712..28251
                     /gene="pyrR"
                     /locus_tag="IW728_000079"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1023467.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28265..29548
                     /locus_tag="IW728_000080"
     CDS             28265..29548
                     /locus_tag="IW728_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1023468.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29561..30487
                     /locus_tag="IW728_000081"
     CDS             29561..30487
                     /locus_tag="IW728_000081"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1023469.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30506..31786
                     /locus_tag="IW728_000082"
     CDS             30506..31786
                     /locus_tag="IW728_000082"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1023470.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31791..32867
                     /gene="carA"
                     /locus_tag="IW728_000083"
     CDS             31791..32867
                     /gene="carA"
                     /locus_tag="IW728_000083"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1023471.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32872..36057
                     /gene="carB"
                     /locus_tag="IW728_000084"
     CDS             32872..36057
                     /gene="carB"
                     /locus_tag="IW728_000084"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1023472.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36111..36887
                     /locus_tag="IW728_000085"
     CDS             36111..36887
                     /locus_tag="IW728_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1023473.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36884..37816
                     /locus_tag="IW728_000086"
     CDS             36884..37816
                     /locus_tag="IW728_000086"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1023474.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37813..38529
                     /gene="pyrF"
                     /locus_tag="IW728_000087"
     CDS             37813..38529
                     /gene="pyrF"
                     /locus_tag="IW728_000087"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1023475.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38536..39165
                     /locus_tag="IW728_000088"
     CDS             38536..39165
                     /locus_tag="IW728_000088"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1023476.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39153..39467
                     /locus_tag="IW728_000089"
     CDS             39153..39467
                     /locus_tag="IW728_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023477.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39505..40146
                     /locus_tag="IW728_000090"
     CDS             39505..40146
                     /locus_tag="IW728_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1023478.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40177..40881
                     /locus_tag="IW728_000091"
     CDS             40177..40881
                     /locus_tag="IW728_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1023479.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40878..41780
                     /locus_tag="IW728_000092"
     CDS             40878..41780
                     /locus_tag="IW728_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1023480.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41938..43644)
                     /locus_tag="IW728_000093"
     CDS             complement(41938..43644)
                     /locus_tag="IW728_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1023481.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            43834..44457
                     /locus_tag="IW728_000094"
     CDS             43834..44457
                     /locus_tag="IW728_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023482.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            44907..45956
                     /locus_tag="IW728_000095"
     CDS             44907..45956
                     /locus_tag="IW728_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1023483.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            45953..46834
                     /locus_tag="IW728_000096"
     CDS             45953..46834
                     /locus_tag="IW728_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1023484.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            46837..47607
                     /locus_tag="IW728_000097"
     CDS             46837..47607
                     /locus_tag="IW728_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1023485.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            47886..48740
                     /gene="aroE"
                     /locus_tag="IW728_000098"
     CDS             47886..48740
                     /gene="aroE"
                     /locus_tag="IW728_000098"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1023486.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            48918..50081
                     /locus_tag="IW728_000099"
     CDS             48918..50081
                     /locus_tag="IW728_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1023487.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            50198..50608
                     /locus_tag="IW728_000100"
     CDS             50198..50608
                     /locus_tag="IW728_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1023488.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            50782..53010
                     /gene="topB"
                     /locus_tag="IW728_000101"
     CDS             50782..53010
                     /gene="topB"
                     /locus_tag="IW728_000101"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1023489.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            53148..53465
                     /locus_tag="IW728_000102"
     CDS             53148..53465
                     /locus_tag="IW728_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023490.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            53625..53861
                     /locus_tag="IW728_000103"
     CDS             53625..53861
                     /locus_tag="IW728_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1023491.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            53858..54256
                     /locus_tag="IW728_000104"
     CDS             53858..54256
                     /locus_tag="IW728_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1023492.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT        17790 a         9484 c        11761 g        15468 t
ORIGIN      
        1 aaaatttttc agctttttta ttcttggaat aacgaggtat tgttttcgat aaaaaaagat
       61 ggtgcagaca aattatgata attaagcata ctatcagata actcaaaatg aagccttctt
      121 tcaaaaaatt attcaaaacc atatattgta ttttttattt gataataaca atgggattat
      181 taagaagtgg cactaaataa aagactagtg tgtatttagc cgatttaatt ataaacaagt
      241 aaatggataa agtatatgta atgataaaag aaaaatggat tttctaattt ttaagctaaa
      301 tgttagatca cacatgtata tgctggatag tcttataaaa aatcatgtaa tacagcttgg
      361 tacatgattt ttatttgtag ctgtttttta catagacaca aaaaagaaac cacgcttgta
      421 gacgtggttt cttttttact atctgtttcc agaagctgag gtgctgctgg atggtgtatg
      481 gtcatttgca gcatccgctc cgctagttgc tggctcactg gaagaagcag gaggagcaga
      541 tgaagacggt gtcgagcttt cttcggatga agaagaagga accgttgttg aagacgctgg
      601 ctcgcttgtt tctgctgtcg tgctttcttt tgaggactca gcagttgatt cgctttgact
      661 agtagagcta gagctttgag tcgttgtctc tgttgttgag cttccaggag tctggacata
      721 gctggaagat ggaatagttg ttgatggtgt aattgcattc gatctagctg tatattgatc
      781 tttgtagtac agttcaccgc ccacacgaat taatccgctt ggcatctccc agtctgtatt
      841 tgtcacgtta gctgaaacat actgcataag ctcgcgataa acatcagagg ctacgtgtga
      901 cgattctgaa gtgacaggtg tcattttttt attgtaacct gtccacacgg aaatcgaata
      961 atttggcgta tagcccgcaa ataggatatc tggatacacc ccagaagaaa taccaagttt
     1021 tgcatactcg tcatctgtgt aattggatgt tccggtctta ccggcttgat ataacccagc
     1081 gatttgcgca ttcgtaccag ttccttcagt gatcgtatct tttaaaatgt ctgtgatcat
     1141 ataagctgtt tctggagaca tggctgtttt tccatctggc tcataagttt cttctgttcc
     1201 gtcttggaag acgattttat taacatattg aggtttgtaa taagtccctc cattggcgaa
     1261 agccgcatag gctgctgcca tttttaagga agaagctccg tattttgtcc catcttgttc
     1321 ttccgtatta ctagagatcg cattcgattg atggatcgta ctgtattcga tcccaagatt
     1381 ttttaagaaa gaagccactt tatctgaacc gacttcattg aataatttga ctgctggaac
     1441 attacgggat aagtaaagtg cttggcgtaa agtaatggtg cccatatatt gattatccca
     1501 gtttcctaca ggagtcgaag tcccttcata gttatacggt gcgtcagtaa tagtttttcc
     1561 agtagaatat ttcaaatatt caaaagctgg tccgtaatct gtgacaggtt tcatcgtaga
     1621 accaaaatca cgggaagtat tcactgctaa gttgttgcct aaagttacgt cttctgcgat
     1681 gtgacgtccg ccaatctggg cttttacttt cccggtattg gtgtcgataa gggtggaggc
     1741 aacttgcatc tcgtcgtctg ggtaagagac atactgatcc gtgttgacga tatcatatag
     1801 tttcttttgg gcgtccagat ccaaattcgt ataaatctct aaaccgtctg tgtagacatt
     1861 cttatctgtt ttttcttgta cttcattgat gacttctttg acataattat cgacgatctt
     1921 agtattgtca tcggattgag ttagttcttg cagaccatcc gttaccggta cattaacagc
     1981 ttgatcatat tctgtttgag agattttttc gttttgaagc atcgtataca aaacagtatc
     2041 tcgtcttttc ttcgcctgat ctggataaac ataaggatca taggcggaag gggcttgagg
     2101 catcccagca agcaatgctg tttgcggtaa agaaagttct gataatttct taccaaagta
     2161 catctcagaa gctgtctcca tcccgtacaa gccgtttgac atatacactt tatttacgta
     2221 gtaggtcaag atctcttgtt tcgatttttt ttgttccaat cttacagcca tccacgcttc
     2281 ttgggccttg cgtttcagag tctgatcctc ggcactggtg gagaagaagg aaagtttgat
     2341 caattgctgt gtcaaagtac ttccgccttg cagtccgcca gaggtaaagt tagagagggc
     2401 agaaccgatg atccgaatag ggtcaacccc gatgtgttta taaaaacggc gatcttctac
     2461 cgacacgatc gcatcttcta atgttttcgg taattcattc gcagaaattt tttcacgctt
     2521 ttctgcgcct aaatcttcga atagttcgcc atcttgtgta tatagtttgg aagatacggt
     2581 agcgtctaat tttttatccg tcaattcggg cgcatctttt gcgtaatacc agaaaagtcc
     2641 tacacctgct aaaaagagga tacagaaaaa tgagagtatt cctaaaagga tcttgataaa
     2701 taatcctttt ttatgtgttc cagaggactt tccagaacct ttgccagaat tcttcttcac
     2761 actcttttta ggagtaggct gtttctgtcg tctagagctt ctagtttgtt catttgccat
     2821 tcttgctttc tccttcaata gataatgttg ataaatagaa gtcaaccgcg tccaaataag
     2881 gaatccgcgg agaaatacca gttttgatct ctattcctag ttcttcgatc aaagataaag
     2941 gtagtgattt gcgtcctttt tccttttggg cgtcccagta agcaatcaaa tgaacactat
     3001 tcaaataaaa acagcgcttc aaagacgaaa accataacaa gacaaagcag accccatttt
     3061 gttcgaggca tgctttcata tgttcgattt gatgggcatg gaagttttta aagggaaaag
     3121 atgttttgtt cttggtttct tttgcttcaa agtccaagta aaagcctcga tagacgccat
     3181 tatagtctgt agttgaggct tgggtaaagt aggcttcctt gatcacagca gcacttctct
     3241 taggatagtc tactttcacg atttggatag gggtaggctt tttatggata accgccaacc
     3301 cgcgggaaag ataatattta ttactttcat tgattgcttc ttcaaagcgc atcccccgat
     3361 tgccgaattc agtgatctga tttctttttt cttgaatttt ttcgacggga gaggcattct
     3421 tataataagg ttggccattt ggatagcgca ataccatctt taatcacact cctaggccac
     3481 attatagcaa aaaagcggat agaaagaaaa ggaatctgag atggataagt caaaagtaat
     3541 ctatgtgtct gggtatcgaa gtttcgaact cggtgttttt aaagaaaatg atcctaaaat
     3601 ccaagttgtt aaaaatgttt taaaaaaaga actccatcag ttagcagaag aagggctgga
     3661 atgggtactt gtcagtggga atttgggcgt cgaactatgg gctgtgcaaa tagtagcgga
     3721 attgaaagaa gaatatccgg aactatctct tggtattttg tatccttttt ctgattttgg
     3781 taataactgg aatgaaggga accaagaaaa aaaacaatta gcagaacaat tagcagatta
     3841 tgtcgaagct gttagccatc aaccctacca atcacctagt cagctaaaga atcacactcg
     3901 ttttatactt gagcatacag tgggctgtct gctgatctat gatgaagaat tccctggaaa
     3961 aacgaagtat ttttttgaag atgcacagaa ttaccaagca gatcatccct acgaaattcg
     4021 gctgatcagt atggacgatc ttcaaaattt tagtgaataa tcgatattgt gtttgattta
     4081 tcaacttttt tcctatacaa tgaaaaaggt agaaatgtta ttacgtaaat gaaaactaga
     4141 ggtgtaaata tggcgaattt ggtttatagt cctaaagaca ttttacaaca agaattcaaa
     4201 acaaaaatgc gcggctatga tcccgttgaa gttgatgaat tcttagacaa tatcatcaaa
     4261 gattatgaaa cttacagcaa agaactcctt gctttacaag aagaaaacga tcgattgagt
     4321 gcaaaagtag ctcaattatc taaaactcaa ggagcagctc aaacacgcgt gcaacaaaca
     4381 gaggcaccaa agagcgcagc agtgaccaat tttgatatct taaaacgctt gtcaaatctt
     4441 gaacgtgaag tatttggtaa aaagcttgat caacaagcaa gtgcagtcaa accagcacaa
     4501 cctaatccaa ataactacac aaatgcagac acaagcttag atgataatga aaaaacacgt
     4561 caattttaat ttttgattaa tgaatttgcg gttttcgggt aatcgcggtc tgacttgatc
     4621 aggctgagga aagtccatgc tcgcacaggc tgtgatgcct gtagtgttcg tgcttagcga
     4681 aaccataagc taaggtactc tttggagtaa cggcaggaaa acaagctaag gcttcggcta
     4741 tgcttaagta tccttgaaag tgccacagtg acgaagcggc tggggaaacc catccggtgg
     4801 aacgcggtaa acccctcgag cgagcaaccc aaacaatggt aggggcgctt tttgtctgga
     4861 agaagaacag gacaaaaagg cagtataact gcagatagat ggttaccgct ttgttttttc
     4921 tccctggaaa agcaaagtac agaacatggc ttatagaaaa ccgcaaacat cagggaatta
     4981 ggtcgtgaca aaagtaatgc gttataagaa acaagaaaaa catttgaaag ctatttttca
     5041 atacttcaga tgaatcgttc ttattacaaa acaaactttt gtatcaccgg ttattcggaa
     5101 tttgagtaaa agtgtggggt aggactgatg tcccacctcc tttttttata aataaggaga
     5161 ttgtgtatgg aaaaaaacag gaagttcaac ctcgtcgcaa ctgcggcaag cgggctagaa
     5221 gcactggttg gtaaagaatt aagagatctt ggaatcgaat gccaagttga gaacggacgt
     5281 gcacgttttg aaggaacaat ggaaacgatc gctacagcta atttatggtt acgtacagca
     5341 gatcgagtaa aaatcgtggt tggggaattc gatgcgtata cgtttgatga attgtttgaa
     5401 aacgtaaaag cattgccttg ggaagatttt cttccactag atgcagcttt tcctgtagct
     5461 ggaaaatcga tcaagtcgaa actatatagc acacctgatt gccaagcgat cacaaaaaaa
     5521 gcaatcgttg aacgtctccg tacctattat caccgtccag cttctgtacc actgacggaa
     5581 actggcgcac attttcagtt ggaagtagct ttgctgaaag accatgtgat ggtaactctg
     5641 gatacgacag gaccaagttt attcaaacgt ggttatcgct tagaaaaagg tggagcacca
     5701 ttgaaagaaa acatggcagc agcacttatt atgttgacaa attggcgaaa agaccgtcca
     5761 ttttatgatc ctgtatgtgg ttcaggtacg ctttgtattg aagcagcctt aataggccat
     5821 aatatcgcac caggattcaa tcgtgagttt gcttgcgaat cttgggattg gttcagtcaa
     5881 gaaatcatgg aaaatgttcg atcagcagcc gaagaaaaag cagattatga tattgaatta
     5941 gatattactg gttcagatat caatggacgg atgattgaaa ttgccaaagc caatgcagaa
     6001 gaaatcggat taggctcttc gattaccttt aagcaacaag cagtcaaaga cttcaaaacg
     6061 gataaagaat acggggtcat cgtggctaat ccgccttacg gagaacgtct aggagaagaa
     6121 gaaactgttc ggaaattata taaagaaatg ggcgatgtat tccgtccatt aaaaacatgg
     6181 agtaaatata tcttgactag cgacttagcg tttgaggaat tttacggaca aaaagcaacc
     6241 aaaaaaagaa aattgtacaa tggtgcattg cgtacggatc tattccagta ttggggaact
     6301 cgtccgccta gaaaaaaaga gaaagacgaa taatcttact aattgattca ttcaattgga
     6361 tgtttggagg aatagggaat gaaagaaaaa gagtttttac aagaagtaaa agaaatcaat
     6421 cttctacaac aagctttagg aatcctcgat tgggatacac aaacaggtat gcctgaaaaa
     6481 gctagtgcat accgaagtga agtagacagc tatctttaca gcatttattt ttcaaaaaaa
     6541 atcggtccaa aaatcaaaga agccattcgc tatttttctg aacatccgga agaattgtcg
     6601 gaagttggaa ctgccgtttt tgccaaagta aaagaagagt atgatttgga acatcgcgta
     6661 ccggaagatc ttatgagaaa atatacagct gccgtttctt cagcacacgg acaatggcaa
     6721 aaagctagag aaacacaaca attttctgat tttcaacacg cattgaatca aaatattgaa
     6781 ttaacaaaac agttgatacc ttactggaga aaagatgaaa agacgaatta tgatgttttg
     6841 ctgaatcaat acgaacccgg gatgactgtc gaaatccttg ataaggtatt tgatcagctt
     6901 aaagaaggaa ttctttcgat ccgccgtacg ctccaagaaa aagggactga acctgaaaca
     6961 gactttttaa atcggagaat gacgaaaaaa caacagaaga gatttgtagt gaaagtaatc
     7021 gaacaacttg gttatgattt tagtcgtggt cgtttagatg acactgtcca cccatttatg
     7081 actggaatta atccgaatga tgcacgaatc acgacacgat ggaacgaaac agatttcaaa
     7141 atggctgttt ttggagtgat ccacgaggct ggtcatggta tgtatgaaca agacatcgat
     7201 gataaatatg cgtatacacc ggtttatgaa ggtacatcaa tgggtatcca tgaatcacaa
     7261 tcgttgttta acgagatcat tattggaagt agtcgcagct tctggaaaaa gcaatatcct
     7321 ttctttcaag aatgtgcaga aggaacattc gatgatattc cttttgacaa attttatcgt
     7381 tcattgaacc atacaaaatc aagtttggtt cgtatcgagg cagacagttt gacttaccct
     7441 ctccatatca tcattcgtta tgaaatcgaa aaaatgattt tcaatgaagg ggtagatgtt
     7501 gctgaactac ctgaaatatg gaatcaaaaa tatgaagagt atctgggaat acgaccaaca
     7561 aatgatgtgg aaggcatcct acaagacgtt cattggtcag gtgcaagctt tggttatttt
     7621 ccctcttatg cattagggta catgtatgcg gcacaattac ttcattcttt gcagcaagaa
     7681 atagatgtgg aaaatgttct agcgtcagac gattattctc caatcaaaaa ctggatgtca
     7741 gagcatatcc atcaatatgg tgcatctaga aaaccaaatc aattaatcat ggatgcaaca
     7801 catgaaccgc ttaatccgca atacctgatc gactatatga aagacctgta ttttaatgta
     7861 tatcaggtaa aataaaatac aatttacaaa gatagctgaa attcatctaa tctatagatg
     7921 ttttcagcta tttacttaag aaagaagtgg aacgaatgga taaattacaa gtgatcgctg
     7981 attatagtat ggaccaatta gcgcatgatc aaacaggaca tgggagcgat cataccaaaa
     8041 gagttgtcaa gttagctgaa aaaattttgg agacggaacc gcaggcagac cgctttgtga
     8101 cgcttgcagc agcttaccta catgatacga ttgatgataa agtcgttaaa aatgagaacg
     8161 aggcaaagca acagcttcgt gtattccttc gctctttgcc aatagtagag gaacagatct
     8221 cgatgatttt ttccattatc gaaaatatgt ccttttctaa aaatctctct gaagcggccg
     8281 aactatctct ggaaggtaag atcgttcaag acgctgatcg tatcgaagca ttaggagcaa
     8341 tcggcatttt gcgtactgct tatttcggcg gtggacatgg gcacccgatt tttgatcctg
     8401 aactgtatcc gcaaacattc aaggacaaag aaaattaccg aaaaggcacg acagtgatca
     8461 atcattttta tgaaaaactg ttcttgctgt ctgataaaat gaatactgat tacggatatg
     8521 aagaagccaa acgaagagaa accttcatgc gtgaattctt agaagaattt tttctggaat
     8581 ggtctattga cgatgaaaat tttactccag acaattggat caaaaataac aaaaaatgat
     8641 ggaggttgtg acagaagtgg tcagctccaa gatatttcgg atcattttcc gtaaaagtta
     8701 cttctggaac actgtttacc aagaggtcgt gagcctgccg ttcagctccg agtcacaagg
     8761 aacaatttca aaaaatagct tctcgtgttt gatgaatatt gtaacttgtg attcggaatt
     8821 ggcaggagaa caccgtttat tcggaagaat aggactgtga caagactttt gtcacagtcc
     8881 ttttttcgaa ttcaacaagt aaaactttta agaaatattc agagagtaaa attaaaaatt
     8941 gctgataaac gaagatggaa attttatgta tttttattgg tctatgcaat aaataaatta
     9001 aatattcaga atacaattga ttttaacgaa agggcttcta tcaatggttg tgttgtattt
     9061 atctgcccag gtagagtaaa atagatcatt atctaaaagg taattgttca tactatataa
     9121 gtcatttgaa agtaaaaaga tactgtaaat aaaaaatgtg gttaaataat tctcataatc
     9181 agtgtaagcg ttatcaaaaa tatagcatac ttgctttgta gatgaatgac acgatatatg
     9241 tttggtatgt tctgcaagac agatgaatat ggaggtggag acaatgtctt ccaaagaact
     9301 aacaatgtat ttagagaaag aattggatca actgaaagaa aaggggttat acaatacgat
     9361 cgacatacta gaaagtgaaa atggtgcttg tatcatcgta gacggaaaaa agatgatcaa
     9421 tctagcatcc aacaattatc ttggatttgc caatcgcgaa gaattgaaaa aagcatgtat
     9481 tgaagcaacc gaaacttatg gtgtaggagc aggagcagtc cgaacgatca atggttcgct
     9541 gaaaatccat cagcaattag aagaaaaaat cgcagaattc aaagggacag aagcagcaat
     9601 cgcctttcaa tctggattca actgcaatat gggcgctatc tctgcagtaa tgacaaaaga
     9661 agatgctatc ttatcggatg aattaaacca tgcatctatt attgacggtt gccgcttatc
     9721 cggagcaaaa atcatccgaa ttaaacatca agatatgaaa gaccttgaga aaaaagcaaa
     9781 ggaagcgata gaaagtaaga aatataaaaa aatcatgtat atcacagatg gtgttttctc
     9841 catggatgga gacattgcac gattgccgga aattattccg attgttgaaa aatatggact
     9901 gatcacctac gtcgatgatg cgcacggctc aggcgtaact ggaaaaggtg caggaaccgt
     9961 caagcatttt ggattaagcg acaaaatcga tatgcaaatg ggcacgttat cgaaagctat
    10021 aggtgttgtt gggggttatg tagctggatc gaaaacacta atcgactggt tgaaagctag
    10081 aagtcggcca ttcctatttt ctacttcgct aactccaggt gcagctgcag ctgcattggc
    10141 ttcgattact ttgatgcaag aacatccgga acttgtcgaa aaagtttggg aaaatgctaa
    10201 ttattttaaa gaagaattga aaaaagtagg ctataacatc ggtatgtctg aaacacctat
    10261 tactccagtc attttgggag acgaaaaagt gactcagact ttctctaaga agctgatcga
    10321 acatgggatt tatgctaaac caatcgttta tccaactgtg cctcttggga caggcaggat
    10381 cagaaatatg ccgactgcgg aacatacgag ggaaatgctt gatgaagcag tagcagttta
    10441 tcaaaaaata ggaaaagaaa tggaaattat ttaactaaat ttagtttttt cgtctattta
    10501 aaattagaga acatgttcac gcatatattg ttaatcatct gaagatagta gtaaaaaaga
    10561 tcagggagga tcatcatgaa aaaagtattg gtgacaggat gtttaggaca aattggttca
    10621 gagttgatca cacggctacg tcaagaacta ggcagtgaaa atgtcattgc gacagatata
    10681 cgcatgcctg aagtcattga gaaaaacgat atctttgaga tcctagatgt aatggattac
    10741 gaaaaaatgc gcagcatagc agaacaatat caagtggaca cattgattca tttggctgct
    10801 cttttgtcag ctgttgcaga agcaaaacca caattagcct ggaatttgaa cgtgactggg
    10861 ctggtcaatg cattggaagt cgctagagaa ttccagctga aattttttac gccaagttca
    10921 atcggcgcgt ttggtccaaa tactccaaaa gatcataccc cacaagatac tcttcagcgt
    10981 cctacaacca tgtatggggt aacgaaggta gctggagaac tactatgtga ttattattat
    11041 gtgaaatatg gcgtagatac tcgtggtgtc cgtttccctg gattgatttc ttatgcaacc
    11101 ttgccaggag gcggtacgac agactacgca gtcgatattt attattcagc aatcaaagaa
    11161 ggaaagtaca ctagtttcat tgatcaaggt acatcaatgg atatgatgta tatgccagat
    11221 gcgatcgatg ccattataaa actcatgaat gcagatcctg atcgattggt tcatcgaaat
    11281 gccttcaata tatcggcgat gtcttttgac ccggaagaga tcaaagcatc tattcaaaaa
    11341 atcataccag attttgaaat ggattatgat gtggatcctg tacgtcagtc aatagcagaa
    11401 tcttggccaa attcactaga tgtttcttgt gcgcaagagg aatggggctt ttcgccgaaa
    11461 tacgacttag atgtgatgac aaaggatatg ttggaaaaac tgaaagaaaa attggtagag
    11521 acggtataaa acaagaacca catccaaaaa gttttttata cttttgggat tggctatggt
    11581 atgaaagggg acaagggatg gaagaatatc aagccacgcc agtaaaagaa gtaaaaatag
    11641 aaaatgaatt aaaacgtacg atgggatttt ttactgcttt atcgactgtt atgggcacag
    11701 tgatcggtgc aggggtattt tttaaagccg caagcgtagc agaagttaca ggatccgcca
    11761 gtttgcatat gttttcttgg tttctcggtg gtatgatttc tgtctgtgca ggactaacag
    11821 gagctgaact ggcagctgct attccagaaa caggagggat gattaaatat atcgaacgga
    11881 tttacggaaa tacagcagct tttttattgg gctgggcgca agtcgtgatt tattttccag
    11941 caaatgtagc agccttgtcg atcattttcg gtacacagtt cgtcaatcta tttggtcttt
    12001 cccagtcaat gattgttccc gtagctgtta cagctgctgt atcgatcctg ttgatcaatt
    12061 ttttaggttc taaagctgga ggggcttttc agtcgattac gcttgtctgc aagcttattc
    12121 ctttgtttgt gatcgtcatt ttcggtttgt tccgacaaga aggagtagac ttccaactgt
    12181 ttccgatcca agcaggagaa aatctttcat ttttctcagc cttgggagcg ggcttgctag
    12241 caacgatgtt cgcatacgac ggttggatac acgtaggaaa tatttcggga gaattgaaaa
    12301 agccggcgaa agatttgcca aaagcaattt ctttaggaat catcggaatc atgatcgttt
    12361 atttattagt aaatgcggtg ttcttaagaa ccgcatctat cgacggagtc gcaggaaaca
    12421 gcaatgcggc aagtgacgtt gcaaaaatga tttttggcgg cttcggtgga agactggtca
    12481 cagttggtat cttgatttct gtttatggaa ccatcaatgg ctataccctt actggaatgc
    12541 gtctccctta tgttatggca aaagaaaaca acctgccttt tagcaagctc tttgctaaac
    12601 ttcatgataa aactaaagtt cctgtagccg caggtatctt agagctagtc atcgctattg
    12661 gcatgatgat gatcggtggt tttgatacac taacggatat gctgatcttt gtcatttgga
    12721 ttttttatac gatggtcttt gttggtgtca ttcttcttcg taaaaaagaa cctgatttgt
    12781 ttcgaccata caaagttcca atgtatccgt ttattccact agtagcaatc attggaggga
    12841 cttttatcgt atcaagcacg cttatcactc agaccttttt agcttcgatg gggatagtat
    12901 tgacacttgc tggtatcccg atttatctct atttaaaacg tagatagcta aaacattcga
    12961 attttattag aactgcactt ctctgccaaa caaaatattt tatttaaatg aggagtacgt
    13021 ggaagaaatg tcaaaagtac tcgttaattt tgatcacgta aatgtcgttt tgtctacgat
    13081 gaaatagcaa agtaaaagac agaaatcggg ctcaaccgat ttctgtcttt tactttgatt
    13141 tgcgtaactg aactattcgg cgtacgccgc gataaatatt taataaaacc aagcaaccag
    13201 aaatgaaaat gggaagccaa atcagataga cagggtagcc aagcacttta gacaaagcat
    13261 gtggagtcat tttatccatt tcaagaagtg cttgctgcca agtctgaaga ttgaagacta
    13321 gcatgaaacc cgaaataatc aggagaataa atcctaaata atgaattgta gttctttttc
    13381 ttacataagc gatcgtagca taaaggaaca gtaaaaaaat aggtacgcca aaaacaaagt
    13441 acatattcag catctcgaca ccccctttgt gtttatctta ctttactttg aaagaaatga
    13501 gaaataatag cctcgttact tgcttgaaac agcaaaggac tgctaagata agagagatac
    13561 aaagaatcaa ggaggagtca tatggcaagt tatatcgaac cgattcgttt tgcgctgatt
    13621 gtttttccat ttttagcctt agcgatctca tctgtttttt ttatttatga atatcgaaag
    13681 tatggaacat ttctagtaac aagggcagtt attttgtatt ctttcgtttt ttatttattg
    13741 tgtgcgtatt ttttggtgat cttaccactt ccggcaaagg agacggttgc ccaaatgaca
    13801 gggccaaaga tggagctgca tttatttgct tcatggcaac atttcagaga caacacggta
    13861 ttggtcttgt ctgaacctag tacctacctg ccggcaatga agcagagtgt atttttggaa
    13921 ccagctttta atgttttatt actcatacca tttggcattt atttaagata ttatttccgg
    13981 ctttctttta tgaaaacgat gattgctagt ttctgccttt ctctattttt tgaattgacg
    14041 cagctaagtg gactttattt tatctaccca agaccatacc gcttatttga tgtgaatgat
    14101 ttagtgacaa atacattggg tggaatcatt ggttttgtcg ttacgccgtt atttacattt
    14161 atgcttccta caagaagacg aatggatgaa ctttcttatg aaaaaggaaa gactgtctca
    14221 ttgactcgac gcttggtcgc ttatttgatc gactggacat ttttatctct tattaatttg
    14281 gtagcggcga ttttctttaa aattgcgttc gattcagggg cagttacttc tcatatctgg
    14341 tggcttttat tagaagtagg ggtatacttt atgattgtac cgtttataac caatggatgg
    14401 acacttggca aaaaaattgt gcgtatccgt ttggtggaag aagaccgcga acgaatttct
    14461 ttaaaagcat tgatgatccg ctacgggtat ctgtactttc ttttttatgg tttaagctgg
    14521 ttggggcagt tgcttcagca aatcatgaat tcgacaacga atacggtgtt gcagatgagt
    14581 gcagtattca tttctctcgg tgtcttgtta ctacaaggta tttttatttt aaatatcttg
    14641 ctatcatttt tgagaaaaaa acggattctg ttttatgaaa aagctagtca cacatatact
    14701 gttagcacga ttggccaaac aaaggatgat gattctcgaa attagctgag caaaaacgct
    14761 ttgtctgtgt tataatgcca aagtcgtgat aatttaaaga gaagaggtgc acgaatgaat
    14821 aattttgata tattccgctt caaaccgttt atcaatgaag cgttaaccgc aaagggcttc
    14881 aaacagccca cagaggtcca ggaacgtttg attcctttaa ttgcaaaagg aaaaaatatc
    14941 attgggcaat ctcaaactgg tacaggaaaa acacatacgt ttttgttgcc tttgtttgaa
    15001 aaaatcaatg cagaaaaaga ggaagtacaa gttgttatta cagctccaag tagggaacta
    15061 gctgcacaga tctatgaagc agctacacaa atcgctaaat tttctacacc ggagatccgt
    15121 gtctcgaatt ttgtcggcgg tacagacaaa cagcgtcaat taaatcgtct gcatcaccaa
    15181 cagccacagg ttgtagtagg cacgcctggt agaatattag acatgatgaa tgaacaagcg
    15241 ctgaagattc acaccgcatt tgcatttgtt gtcgatgaag cggatatgac attggatatg
    15301 ggttttttaa atgaagtaga ccaaattgca agtcgattac cagaaaagct gcagttttta
    15361 gttttttctg ccactattcc agaaaaactt cgtccattct tacgaaaata catggaaaat
    15421 ccaatcattg aagaaatcaa accaaaagca gttatttctg aagacattga aaattggctg
    15481 atttcaacaa aaggtaaaga tgagaatcaa atgatttatc aactgctgac aattggtcat
    15541 ccttatttag caatcgtatt tgccaataca aaacagcatg tggatgaaat tgccgattac
    15601 ttgaaaaacc aaggcttgaa agtagcaaag attcatggag acatcacccc aagagagaga
    15661 aaacgagtga tgcgacaagt acagaatctg gagtatcaat atgttgtagc gactgattta
    15721 gctgctagag ggatcgatat cgaaggtgtc tctcatgtta ttaatgcaga aattccagaa
    15781 gatctagact tctttatcca tcgtgtcgga cgtactggac gtaatggctt aaaaggaacg
    15841 gccattacgt tatattcgcc aaatgatgag caagccattg atgcgataga aaaattgggc
    15901 gtctcatttg aaccaaaaga aatcaaaaac ggggaaatcg tcaaaacata cgatcgtaat
    15961 agacgtgcca aacgagaaaa atcaaaagat gtattagatc ccacgctgat tggtcttgtg
    16021 aagaagaaaa agaaaaagat caagcctggt tataagaaaa aaatcgattg ggcaattgca
    16081 gaaaaaaata aaaaagaacg caaaatcgaa cgcagacagc aaagccgtac tgctcgtaaa
    16141 aataagaaaa attcacataa ataaaaaaca agaggttgtg acaaaaggcg tgtcacaatc
    16201 tcttgttttt aagctagact ttttttcatt ccgatacaaa gtacttcatc ttcaataaaa
    16261 ggttctgtgc aagtatgata cccttgtttt tcataaaaat ctacagccgt catttcagct
    16321 actacataac tagtctgaca accttctgaa accgcccgtt tttcagcagc ttcaagcaac
    16381 tgaccgccta acccttttcc tcggtattct tttgctacgc aaaatcgttc cggcttgaag
    16441 caagtaggtg ttttcttttc ataacgcaaa gtagctactg gtcttttttg atcaagaaat
    16501 agataatagg tttgttgcgg gatatctttt ccgtcgaatt ctagttctgc aggaatccct
    16561 tgttctaata caaaaacttg atatctcaaa aagcagctag ctgcgcgtat ccattcatcc
    16621 gctccaacta tttttttcat catattcctc cttgtcatga atagcagaga tgtattgatt
    16681 cctttattct tatcgtacca aaaatcgtgg agtatgacca aaaggaaatg aaaataaaaa
    16741 ttgagatatt gacagtgatc aatagaatgc gtatactaaa taagtacgaa aaggatatga
    16801 tttgtttttt aagactcaca gaaaattctt tcttggctga aaaaagaata gtcactaatc
    16861 aaattgctcc ctttttctat tttggtagaa ataccgacgc agctcagcgt tatcgagttt
    16921 aagaggtaat gattacacat cattgcaatc aaggtggtac cgcgagtttt cgtccttggc
    16981 tgtgatggtg tttttttgta tttaatttat agaaagcagg taatgacatg aaacaattaa
    17041 gtagtgcaca agttcgacaa atgtttcttg atttcttcca atctaaagga catacgattg
    17101 aacctagtgc gtctttgatc cccgtgaacg atccaacctt attgtggatc aactctggtg
    17161 ttgcaacatt gaaaaaatat tttgatggtt cagttgtgcc agaaaatcca agaatcacaa
    17221 atgcccaaaa atctattcga acaaatgata tcgaaaatgt cggtaaaaca gcacggcacc
    17281 atacgatgtt tgagatgctg ggtaattttt cgattggtga ctatttcaaa aatgaagcca
    17341 tccactgggc atgggaattt ttgactgatc caaaatggat ggcattcgat ccagaaaaac
    17401 tttatgtaac agtttatcca aaagatacag aagcaaaacg catctggcat gaagaagttg
    17461 gcctgccact agatcacatt attgatgtag aagacaactt ctgggatatc ggtgcaggcc
    17521 catgtggtcc ggatacggaa attttctatg accgtggaga agcttataac gatttagcag
    17581 aagacgatcc tgaaaactat ccaggaggag aaaatgaacg ttatttagaa atctggaact
    17641 tagtattttc agaattcaac cataagcctg ataacacatt tgaaccactt cctcataaaa
    17701 acattgatac ggggatggga ttggaacgca tggtctcaat cgttcaagat gcgtcaacga
    17761 actttgagac agacttgttt atgcctatta tccacgccgt agaaaagcta agcaaacaag
    17821 taaaatatgg tgaaaaccca gtaacagata tttcttacaa agtaatcgca gatcatattc
    17881 gcgccctttc atttgctatt ggcgacggtg cattgccatc gaatgaaggg agagggtatg
    17941 tcttacgccg tttgcttcgt cgtgctgtca tgcatgggaa aaaattaggt attgaacaag
    18001 cattcttata tcgtttagtc cctgttgttg gggaaatcat ggtaagttat tatcctgaag
    18061 tacttcaaca aaaagaattt attgaaaaag ttatccgtac agaagaagaa cgtttcaatg
    18121 aaacaatcaa tgaaggtttg gaaatcttaa atcaagtgat ccaagaagta aaaaacgaaa
    18181 acgggacgac tctagcagga aaagacatct tcaaactata cgacacatat ggcttcccgg
    18241 tagaattaac ggaagaggtg gcagaagatg aaggactgaa agttgatcat gctggttttg
    18301 aaaaagaaat ggatgcacaa cgcgaacgtg ctcgctctgc ccgcagtact gaaaaatcaa
    18361 tgggggtaca atctgcttta ttgacagata tcaaagtaga aagtaaattc attggataca
    18421 cagaacaaga atcagatagt caattattgg tcatcgttaa aggtgagtct cttttgaatg
    18481 aagtcgcaga aggtgaagct caaatgatct ttgatcaaac acctttctat gcagaaatgg
    18541 gtgggcaagt agcggatcaa ggatggatca tgaatgaagt aggcgaaatc gtagcagttg
    18601 ttacagatgt tcaaaaagcg cctaatggtc aatttatgca caccgtagaa gtaaaagcac
    18661 caatgagtga aggacaaacc tatcatctat tgatcgatca atcgatgcgc aatcggatca
    18721 tcaaaaacca tacagcaacc catctactgc acaaggcatt gaaagaagta cttggcgagc
    18781 atgcaaatca agctggttct ttagtcgctc ctggacattt acgattcgac tttacccact
    18841 ttggtcaaat cacttcagat gaattagctg aaatggaaag aatcgtcaac gaaaaaatct
    18901 gggaagctat cccagttgaa acaattgaaa cagatatcga tacagccaaa aacatgggtg
    18961 caatggcttt gtttggtgaa aaatacggac atgacgttcg agtagtaaat attggcggct
    19021 ggtcaatcga actgtgcgga ggcacacatg tagcaaatac agaaaacatc ggaatcttca
    19081 aaatcgtttc tgaatcagga attggtgcag gcgtgcgacg aatcgaggct gtaacaagta
    19141 aagaagcgta cgaactactt gaagaagaag aaaaacaact aaaacaagta gcaacaattg
    19201 ttaaatcacc tcaattgaaa gaagttgttt caaaagcgga acaattgcaa cagcaagtaa
    19261 gagagctaca aaaggaaaat gaacaattag ctagcaagtt agcgaatcaa caagctggtg
    19321 atgtattcaa agatccagaa acgatcaatg gaaaaacaat cattgcagca caagtaaaag
    19381 tgaaagatat gaatcagctg cgtcaattag ctgaccaatg gaaacaaaaa aatatttcag
    19441 atattcttgt tttagcaaac gaacaagaag gaaaagtcag tctgctagcg gcaatgacga
    19501 aagaatcaaa tgaagctgga ctaaaagctg gcgacttgat taaagcaatc gcaccgaaag
    19561 taggcggtgg cggtggtgga cgtccagaca tggcacaagc tggcgggaaa aatcctgctg
    19621 gtattccaga tgcacttaaa gctgcgaaag aatgggttgc gcaacaatag ggcgtttacg
    19681 tttgacagaa aaacaaaagt tcaaacattg aacgaaacat gattatgggg gcgaagacaa
    19741 acaagtcttg ccctcatact tttttcaatt ggatggcgca tctaatcaaa tggtcggatt
    19801 cggatggtct ttagttgacg aaagtgacag aaatttttac gattagtata aataaaaagg
    19861 ggtgaaaaaa tgaatagtag aaataagacc tttcgactag tcttacgtgc gatcttgtta
    19921 gcaatcatca tcgtacaagc gatggttcct tggctggggt ttattccatt aggattcatt
    19981 agtttaacaa ttatccatat cacagtaatt attgctgcag tagtattagg cccaaaagat
    20041 ggcatggtga ttggcttgtt ttggggaatc gctacaattg ttcgtgccta tgccatgcca
    20101 acaacgccat ttgatacatt agtgttcacg aatccgatta tctcggtagt tcccagagta
    20161 cttgttggct tagtagcagg cttggttttc cactggattt atcaaaaaaa taaatcaata
    20221 acgatcagct cagcctttgc aggggtactg ggttcattgg tcaatacagt cttagtgtta
    20281 ggattcatgg gattatttta tacaggtgca acggcagatg cctatggtgt agactctgct
    20341 cttttattta aaaccttagc aggggttgca gcaatcaatg ggataccaga agcaataggt
    20401 gcgggaatca taacaccatt actagcaaaa gcgttatttg cagcaacacc gttgaaacca
    20461 gaataatgac catcttttaa tagtaatagg acgcacgata gatagaaaaa ggaagagagg
    20521 agaggaaaac aatcgtcctc tccatctttt tacctgagca acaagcaaac tagaaggggt
    20581 aaaaaacttg gctaagaaac aagaagaaaa aaagaaacaa ataccgcaac agcggacaaa
    20641 aaaaggagta ctagcgggta tcttaggatt ggttacgaat ctgatcttgt ttgtctctaa
    20701 gctaatgatc gggctttttt caggaagtgt ctccattatg gctgatgcag tgaacagtct
    20761 atcagatact gcttcatcca tattgacttt gatcggattt aaaattgctg caaagccagc
    20821 agatcaggaa catccttatg gacacgaacg cttcgaatat atcagtgggt tatttgtttc
    20881 gatcatcatt acttatgtag gatttcaatt tttagagtct tctgctgaaa aaatcatcca
    20941 tccagaaaat gtctctttaa cacccatcgt cttttttgtc ctaatcattt ccatcttttt
    21001 gaaatttgca caaggcaaaa tgtatcaggt tattgcgcaa tccattgatt ctgaaacatt
    21061 gagagctaca gcaacagata gttataatga tgtctttact acactggctg tattgctttc
    21121 agcagcaatc gaatgggtaa ctggatggcg tattgatgga tatataggtt ttgctttagc
    21181 attatatatc ctttatagtg gattgacgat gcttcgaaat tttgtatacg aactattagg
    21241 aagtcgccca acgccagcag agattgaaga aatggaagag catttaaatg cttgcgatga
    21301 tatacttggc tatcatgatt tattggtgca taactatgga cccaataaac gctttgcatc
    21361 agtccacatc gaagtagatg aatcaatgaa tttgaatgtg gctcataaga ttattgatct
    21421 gatagaaaaa gactttaaaa agtctttgga tgtggaactt gtctgtcact tggatccaat
    21481 gcctgttgat aacgaaaaat actataatat cttgcggcag ttaaagcaaa ttcttcttga
    21541 aacaggtaca gggcttaata ttcatgattt tagagtaatt catcaaggaa aaacgctgca
    21601 atttgatgta gtggtccctg agaaattcca gtttgttgat acagaactag aaagaatctt
    21661 acgtagtcaa atagaagaaa aaatcggaga ttaccaattg gaaattacgt ttgatcacaa
    21721 ttatctttta tattaaatga gaaactataa aaagactcct taaagttttt aatttaaaga
    21781 gtcttttttt cttttaattg tttatggaga gcctgacgtg caagattatc tgctccttta
    21841 tttttgtttt cgggcaacca ttgcaggatc aataaagaaa attcggatag aagttcttgg
    21901 attctttgaa ggtagggaag gaagagctcg ttcttcgttg cgtttttgtc gaccgtagaa
    21961 actagtgttt tgctgtctga aaaacagaaa atcgtttcat tctgtaagtc gtttttcttt
    22021 agaaattcta aagtttttaa aaagacttcg aactctgctt gatggtttgt ccctatagaa
    22081 agcgggatag atagctgttc ttgtttacct tgatagacaa tcaagattcc acctccacta
    22141 ggaccagggt tgccttttgt agaggcatca atgtatactt ttaacataaa aagagattct
    22201 cctttttgtt caaactaatg ttatggtaag attatatcat tactgaatcg gtgagaggtt
    22261 agagaatgaa aaaaaatatt cgatggcagc ctgaactggc acatacgatc atctattggt
    22321 cattgacctt gatcattctg ttttttagtc tgattctttc tttagaaaac acaggtcctt
    22381 attggaaaag caattttgtt ttaggtattt ttcttgtttt tctatttcta ggtttgcggc
    22441 gttcttttat tttaaaatca aactctgtga ttatacgtta tgctgctttt tggaaagacc
    22501 gagaaatatc tgtcggacag attcgatctt tgacgccaac taatactggg gtgcaaatga
    22561 tgttgcaaga tgagaaagcc ccatctgttt tcatgatgcg aaaaaaagat caagtagtcc
    22621 tttttgatca ttttgaaaaa aacggaccca aatcagaatc aattccttat ccttatacga
    22681 aaaaaagaaa aagctaattt taaaaatcag aacctaaaaa aacgaatttt tacaggaaaa
    22741 gaaaagagtg agacgtacgg ttcatcccgt ttagcgtctc actctatttg tttatctatt
    22801 catgcatact agtatcggta gtctgaacgt tgcctttctt gacatgggca gcttgtgttc
    22861 ctcgattccc ttcgatgatc tcaaattcga ccacttgatt ttcttccaaa gttttgaatc
    22921 cctcttcttc aatcgcagta aaatgtacaa aaatttcttc tgtgtcatca taactgataa
    22981 agccgtatcc ttttttgttg tcaaaccatt ttacaattcc tgtggtcaaa gcactcacga
    23041 cctttctaaa gtctgatcat agccttcatt ataaagagta tttataagta cgtcaaataa
    23101 attaaaaacc tgtaatttta tgtttttttt ctgaataaaa aaaggtataa ttggcatatc
    23161 ttaacaaaga agatattttg ttgaataaag ttcgtgaaaa ggggtaatca aatgaagaat
    23221 gatctgcaaa tttcacaaga aacagagcta gaaccgattg ttgaagtggc tagtaaatta
    23281 gatcttaagg aagacgattt ggaactgtat gggaaatata aggcgaaaat caatttttct
    23341 ggaatcaatc gatcaaagga taacgctgag gggcatttga tcttagttac ttccatcaat
    23401 ccaacaccag ctggcgaagg aaagtcaacg atcactgtag ggttaggaga cgcgctcaat
    23461 aaaatcaata aaaaatctgt tattgcattg cgagaaccct cacttggtcc tgtgatggga
    23521 atcaaaggtg gtgctgcagg cggaggatat gcacaggtat tgcctatgga agatatcaac
    23581 ttgcatttta ctggtgacat gcatgctatc acaacagcta ataatgcatt atctgctctt
    23641 ttagataatc atattcatca aggaaatgaa ttgaacatcg atgcccgccg agtgatttgg
    23701 aaacgggtag tcgatctcaa tgatcgtgaa ctgcgtaaag taattgttgg cttgggtggg
    23761 ccaatccaag gtgttccaag agaagatggg tttgatatca cagttgccag cgaaatcatg
    23821 gctatcttgt gtcttgcggc agaccttgaa gatttaaagg ctcgtcttgc aaggatcgtt
    23881 gtcggctata catttgaccg tcagcctgtg actgctggag acttgaaagc agaaggcgct
    23941 ctggcattac tgctgaaaga tgcaatcaag ccgaatcttg tacaaacaat ttacggaaca
    24001 ccagcttttg tccacggggg accgtttgcc aatatcgctc atggctgcaa tagtatttta
    24061 gctaccaaga cagcattgag attagcagat tacgttgtca cagaggcagg atttggtgca
    24121 gatcttggcg gagagaaatt tttagatatc aaagtaccta acttgaaaaa agcgccggat
    24181 gctgtggtga ttgtcgccac tgtccgtgca ttgaaaatgc atggcggaat gaaaaaagat
    24241 gaacttaaaa atgagaatct tgatgctttg aaagtaggtt ttgcaaattt aaagcgccat
    24301 atacgtaata tggaacaata tcagctacct gtgatcgtcg caatcaatga attcgtaaca
    24361 gatacagaca atgaactcac actgttggag cacttgtgtg aaaatcaggg gattcttgcc
    24421 aagagagcga gtgtatgggc agatggagca gaaggcggcg ttgatttagc aaaagctgta
    24481 gtacgattga ttgatagaaa agaagctaat tacaagccat tataccgcct agaagaaacg
    24541 atacaagaaa agacagaaac aatcgtcaag aaaatttacg gaggaaatgg tgttgttttc
    24601 agcaaaaaag cgcaaaaaca aatagaagag tttacgaaga atggatggga caaactgccg
    24661 atttgtatgg caaaaacaca gtattcattt tctgatgatc cttcactgct aggtgcaccg
    24721 gaagatttca ccatcacgat ccgcgaaatc atcccaaaat taggggcagg atttttagta
    24781 gccttgactg gagatgttat gacgatgcct ggtttgccta aaaaacctgc tgcattgaat
    24841 atggacgtga caaatgacgg agaagtgttg ggattgtttt aagcttgatt ataatctacc
    24901 atcgaaggag ctgtattttg actacagctt ctttttttag acgataggga attaattttg
    24961 tttacactag gatcattctt tttctttatt gtattttgag ctatactaaa gcataatagg
    25021 agggtatgat gaaaacgaca tttataagaa aaaccggttt ttacggtatg ggtagctcga
    25081 ttcagctaag aatcaacaat gaaaaacgca gtgttctcag tcatgaacaa aagataacgc
    25141 ttgatctgga agtgcccttt acgatgcagg tcacctttta ttggctaaaa agccccgttt
    25201 atacagtgaa agaagcaaag cctgcttatg tgatcacaat gaattacttg attcttcaaa
    25261 tctatccgtt tttatttcta gcaactgggt tgggggccgt tttcctacaa aatttatttt
    25321 tcagtttttt tgcagttatt gtgttgattg cgttcttttt ctatataaaa aaccgggcat
    25381 atttgatcaa ggaggaaaat tatggcaaac tctgatcttt ttttaatcag tttcaatcga
    25441 atcgaaaaat ggatgagaga cgagatggga aatgcacgaa atatgggatt taccgaactt
    25501 gtcagacgcc tagctcaaag aaaacaatta atgatcagaa aatacgaaga tgatctttta
    25561 cagcttgctc agttaaggaa tgcgatcgtc catgatcgga tcgcagtcga ttttgtcatt
    25621 gcagagccaa atgattggac agtcaagcga atccagctga tcgaacaaga actgattcag
    25681 cctgaaaccg tgctgcctcg atttgcaaaa cgtgtaacag gttttgaaca agatcttccg
    25741 ttacttgaac tgttaaagat agtagcagaa aaaaggtatt cacaatttcc gttatacaat
    25801 aaaggaaaat ttgtagcatt gattacttta cgtaatatcg ggttttggct agcaaaagaa
    25861 agccaaaaag gtccggttga tttaacaaat aaacgagcgt tggatttgat catccaaaac
    25921 ggaaagtaca caaattatca ttttgttcca gctaaaacac atatttacga ggttgaagcg
    25981 atgtttcgtg agcaagggac tttggaagcc gtacttatca ctaaagatgg gaatcccgat
    26041 ggtaatctgc tcgggatcgt acgacctcgt gatatttata aacaaataga aaaggattga
    26101 ttttttgtta attatttatt ggatattcag cgctttgcta gtaggagtgg atcagttggt
    26161 caaatggtgg atcactgata atctagcgct tggagagacg aaaaatctta taccaggtat
    26221 tttgtcactg aatcatattc gaaacactgg agccgcatgg agtatgctag aagggaagat
    26281 gtggttcttt actatagtga cactcattgc agtcgttgtg attctcacat tgatgattaa
    26341 aaatcgtgaa aaaggtaatc gctggttcat gattggattg agtttgattc tagctggtgc
    26401 gattggcaat tttatcgacc gtattcgtct tggctatgta gtagatatgt tccagacaga
    26461 ttttatgagc tttccgattt ttaacgtagc agatgtgaca cttgtatgtg gagtgatttg
    26521 tatgttaatt tatattattt tagatgaaaa ggatcaaaga aaaaaatgac agaacttaaa
    26581 acgacgattc accatgaatc tggtaggatc gataaagtat tgactaattt attcaccgat
    26641 tacagtcgtt cgcaaattca gctgtggctg aaaaatggag cggtttctgt caatggacag
    26701 ccagtcaaag caaattacaa agtaaaacaa ggagacgaga ttactgtctt gatccccgaa
    26761 ccagaaacat tagatgtggt cccagaaaat atcccgttga cgatcgttta cgaagatgaa
    26821 gatgtagcgg tgataaacaa acctcaagga atggtcgttc atccttcagc aggtcatacg
    26881 catggcacaa tggtcaatgc gctgatgtac catatcaagg atctttctac gatcaacggc
    26941 gttattcgtc caggtattgt tcaccgaatc gacaaagata cttccggact gctaatggtt
    27001 gccaaaaatg accaggcaca cgaatcatta gctcagcaat taaaagataa gacctctttg
    27061 cgcaaatatg ttgctttagt ccatggagag attcctcacg aaaaaggaac catcaatgcg
    27121 ccaatcggcc gttcgaagga aaaccggaaa atgcaggcga taagagaaga tgggaagtcg
    27181 gctgtcacac attttactgt attggaacgt ttcgaaggat ttactctggt tgaattaaaa
    27241 ctagaaaccg gaagaacaca ccagatccgt gtgcatatga agtatatcgg ttatccgtta
    27301 gctggagatc cagtatacgg accgaagaaa actttgaaag gcaatggtca gtttctccat
    27361 gcgcaaatgt taggttttac gcacccgact acaaacgaaa agatgatctt tgaagcacct
    27421 cttccagaag tttttgaaaa aactttagaa aaattaagaa aaaacattgc attttaattt
    27481 agataacggt atagtaatta acaacgaaag cataaaactt aatagaattc ctttaagttt
    27541 agtcccgtga ggctaagaag ggtgggagca aggatacgta tggtgcgcca taagtagatt
    27601 gctcattgac atagtatagc tatcagtgta tcagtatacg ctcagtctaa acattcctcc
    27661 tgtcctctta gggcaggagt ttttttatgc aaaaaagtag ggggaatgaa aatgcaagca
    27721 aaagaagtag tagatcaggt aaccatgaag cgagcattga ctcgaattac ttatgagatt
    27781 atcgagagaa accatagtat ccaagatatc gtattggtag gtatcaaaac acgaggaatc
    27841 tacattgcat caagaattgc cgaacgcctg aagcagctag aagatatcga tattcctgta
    27901 ggagaactgg atatcacttt ataccgagat gacaaaaaag aaaatccaga agaaccggaa
    27961 cttcattctt ctgacatccc agtttcctta gaaggaaaag aagttatttt gattgatgat
    28021 gttttgtata caggacgaac gattcgagca gcaatggacg cagtaatgga ttttggcaga
    28081 ccaagaaaaa tttcattagc agtcctggtc gatcgtgggc atcgagaatt gccaatccgt
    28141 gcagattatg ttgggaagaa cattccaaca tctcgggcgg aagagatttt agtagagatg
    28201 caagagcttg atggacaaga tcgcatcatg attttgaaag aggaagatta gaggagagag
    28261 aaacatgtcg gagaaaaaag gatttcataa tgatgacgtg gtgttagata tccacgatcg
    28321 cccaacccca ggacattggg taggattaag tttacaacac ttgttcacga tgttcggagc
    28381 aaccgtgtta gtccccatct tagtaggtat cgatccaggt attgcgctag tcagttcagg
    28441 actaggtaca ttggtgtact tatttactac taaaggaaag atacctgcgt acttaggaag
    28501 cagcttcgct ttcattgcag cgatgcagct tttgatgaaa acagatggtt atccagcgat
    28561 tgcacaagga gcagtcacaa ctggattagt ctatttgatt gttgcgctga tcatcaaaaa
    28621 gatcggttca gcatggctgg ataaaatctt gcctccaatc gtcgtcggtc cagtcatcat
    28681 ggttatcggg ttgggacttg cggcaaatgc agcaacaaat gctatgtata atcagggcac
    28741 gtatgatttc aagtatattg ctgtagcgct gatcacatta ggattaacaa ttttcttcaa
    28801 catgtggcta agaggttttc tcggattgat tccgatttta ctaggtatta ttagcggata
    28861 catcgtagcc ttgctgtttg gaatcgtgga tactaagcca atcgtagacg ctgcctggtt
    28921 tgctgttcct aactttgaga ttccctttgt acaatatgaa ccaaagctat atcttggagc
    28981 aatcacaaca atggcaccca tcgcttttgt gacgatgaca gagcatatcg gccatcttat
    29041 ggtattgaat aaactaacta aacgtaattt ctttgaagaa ccgggtcttc ataagacgtt
    29101 gacaggtgat ggattagcac aaatcgtggc agcctttgtc ggaggtcctc ctgtaacaag
    29161 ttacggtgaa aacattggtg ttctggctat cacacgtgtc catagcgtgt ttgtgatcgg
    29221 tggagctgca gtatttgcag tcattttagg attcgtagga aaattaagcg ctttgatttt
    29281 aagtatccct ggaccagtca tctcaggaat cagttttatc ttatttggag taattgccgc
    29341 aagtggaatg aagattttaa tcgagaacaa aatcgatttt gataaaaaga aaaatctgtt
    29401 gattgcttca gttatcttag tagtaggaat tggcggactc gtctttgaag taggcacatt
    29461 tacattgtcg gcaatggcat tagcaactgt acttggcatc attttaaatc ttatcttacc
    29521 agaaacttca cgaagcgaag aaaactaagg aggaaacatt atgatcatca aatcagaacg
    29581 tatcagctta aagcatttat tgaccgttga ggcacttact gatcaagaag ttatgggctt
    29641 gatccgcaga gggcaggaat tcaagcaagg tgctaattgg actccgcaaa agcgccagta
    29701 ctttgctaca aacctctttt tcgaaaatag tactcgcaca cataaaagct tcgatgtagc
    29761 ggagaaaaaa ctagggctag aagtgattga atttgaagca agcacaagct ctgtccaaaa
    29821 aggggaaaca ctatacgata cagtactgac gatgtcagca ttaggcgtag acgtagcggt
    29881 tatccgtcat ggtgatgaga actattatga cgaactgata caaagtaaaa caatccagtg
    29941 ttcgattatc aatggaggag acggaagcgg acagcatcca acccaatgct tgcttgattt
    30001 gatgaccatt tatgaagaat ttggtcattt tgaaggtctg aaagttgcca tcgtaggtga
    30061 tatcactcac tctcgcgtag ctaaatccaa tatgcagatg ttaaaacgtc taggcgcaca
    30121 agtattcttc tcaggtccta gagaatggta cgacgatgaa ttcgaagtgt acggacatta
    30181 tatgccattg gatgaactac tagatcaagt tgatgtcatg atgatgcttc gggtgcaaca
    30241 cgaacgccat gatggaaaag aaagtttctc aaaagaaggc taccatcagg aacatgggtt
    30301 aacagttgag cgtgcgaaga agttgcaaaa acatgcaatc attatgcacc cagcaccagt
    30361 taaccgtgat gtagaattag ctgattcatt agtggaagga ttacagtcgc ggatcgttca
    30421 gcaaatgagc aatggagtat tcgtcagaat ggcaatctta gaagcagtgc taaatggaaa
    30481 agcgtaatta taggaggatg aaaaaatgaa aacactcatt aaaaacggtc agatcaatac
    30541 acgaaaaaat atgacgacac cagcagaaat ttggatagaa gatgggcgaa tcaaagcaat
    30601 cggtacagga ttctcagaag ccgaatttga tgaagtgttt gatgcaaagg gacaattgat
    30661 cacacctggt ttagtggacg tccatgttca tttgagagag ccaggattta catacaagga
    30721 gacgatcgaa gctgggactc gctcagctgc tcgtggcggt ttcactactg tatgcgcgat
    30781 gccgaattta aatcctgtac ctgatacggc agaaaagctg agacaagtat atgacatcat
    30841 tagaaaagat gcagttgtga aggtattgca gtatgcgcca atcacagaaa atctgcgaag
    30901 tgagaaatta gttgatcaag aagcactgat tgaagaaggt gcttttgctt tcacaaatga
    30961 cggtgtaggt gtccaaacag ctgggacgat gtatctggct atgaaagaag ctgcaaaaaa
    31021 caataaagcc ctagtagccc atacagaaga tgaatcttta ttatttggcg gcgttatgca
    31081 cgctggcaag aaggcagaag aacttggtct tcccgggatc ttaagcgtaa ccgagtcttc
    31141 tcaaattgct agagatctgt tgctagctga agcaactggt gttcattacc atgtttgcca
    31201 tgtttctact aaagaaagtg tccgtgtaat cagagacgca aaaaaagcag gaatccacgt
    31261 aacagcagaa gtttcacccc atcatcttat tttgatcgac gaagatattc ccgaagattt
    31321 cggcttttgg aaaatgaacc cgccgctaag aggacgggag gacagagaag ctttgatcga
    31381 aggcttgctt gacggtacga tcgattgcat cgctacagac catgcccccc atggattaga
    31441 agaaaaaagc caaagtttca tgaaatcacc atttggtatc gtaggaagcg aaacagcgtt
    31501 tcaactgatc tatactcatt ttgtagaaac tggtcgattc acgttggaac aagtgatcaa
    31561 ttggttggca gttaaaccgg ctgagatttt tggtttgaat gcaggaacgc taacggttgg
    31621 tgcccccgct gatgtagcag tttttgatat tacacaaact tgcacgatcg ataaagaaga
    31681 ctttttatca aaaggggaga atacaccttt tatcggctgg aaagtaaaag gcgaaacaca
    31741 aatgactttt gtcaacggaa aacttgtgtg gcagaaggga gaataaaagt atggaacgtt
    31801 tgcttatatt agaagatgga acagttttcg aaggaaaggc ttttggtgca gatgctagtg
    31861 tcttcggaga aatcgttttt acaacaagta tgactggtta tcaagaaact atcactgatc
    31921 aaagttttaa cggtcaaatc attacattta cctatcctat ggtgggtaat tatggggtaa
    31981 atagagatga ttatgaatcg atttctccaa catgcaaagg agtagtagta aaagaacatg
    32041 cacgtgtagc ttcaaactgg cgaaaccaaa tgacgctaga ttcatttttg aaacaaaaag
    32101 gaattcctgg aatatcaggt atcgatacgc gcgcccttac tcgcaaaatc cgtgaagtcg
    32161 gaacgatgaa agctagtata gttgatgctg gagattcctt cgaacatgca tttgatcagc
    32221 tgaaagcaac cgttctagca accaatcaag tgcagcaagt ctcaactaac aaaccttatc
    32281 caagtccagg aacaggtcgg aaagtcattg tcattgactt cggcttgaaa catagtattt
    32341 tacgagaatt gagtaaaaga aactgtaacc tcacagtact tccctataac acagatgccg
    32401 agacgatttt agcgctatca ccagacggag tcatgctgac gaatggacct ggtgatccaa
    32461 aagatgtgcc agaagcaatc gaaatgatca aaaaaatcca aggaaaagta ccgatttttg
    32521 gtatttgtct cggtcaccag ttatttgctt tagccaacgg tgcagatacc tataaaatga
    32581 aattcggaca tcgaggatta aatcacccgg tacgagaagt agcgaccgga agaattgatt
    32641 ttacttctca aaatcatggc tatgcagtcg atgaagccac gattgatcca gaaaaactca
    32701 tcgtaactca tgtagaagta aatgacggaa cagtagaagg agtcaaacac agagattatc
    32761 ccgctttcag tgtgcagttt catccagatg ctgcccctgg tccgcatgat gcactacatt
    32821 tatttgatga atttatggaa atgatggacg caggaaagga gcagtaaaca gatgccgaaa
    32881 cgtacagata tcaagaaaat tatggtgatt ggatctggtc cgatcatcat tggtcaagcc
    32941 gcagaatttg actacgcagg aacacaagcc tgccttgcct taaaagaaga aggatatgaa
    33001 gtggttttag taaattctaa tcctgcgacg atcatgacgg ataaagaagt ggcagatcaa
    33061 gtatatatcg aaccgatcac acttgaattt gtatcaagga ttcttcgaaa agagcgtcca
    33121 gatgctctcc ttccaacttt aggcggacag acaggcctca atatggcaat ggagctggca
    33181 gcatcaggta ttttagaaga attgaacata gaacttctag gaacaaagct ttctgcgatc
    33241 gatcaagcag aagatcgtga tctattcaaa cagttgatga aagaattgaa tcagccaatc
    33301 cccgagtctg aaatcgtcaa tacagtagaa gaagctgtag attttgcaaa taaaatcggc
    33361 tatcctgtca tcgttcgtcc agcctttact ttaggcggaa ctggtggagg tatgtgtgaa
    33421 aatgaagaag aattacgtat cgtatcagaa aatggattaa gtctttcccc cgctacccaa
    33481 tgtttgattg aacgaagtat tgcaggtttt aaagaaattg aatatgaagt gatgagagat
    33541 tcggctgata atgcaatcgt tgtctgcaat atggaaaatt ttgacccggt gggtatccat
    33601 acaggtgatt ctatcgtatt tgcccctagt caaacattgt ctgaccatga atatcaaatg
    33661 cttcgtgatg cttctctttc gattattcga gcacttaaga tcgagggagg ctgtaatgtc
    33721 caactcgctc ttgatccaca tagttttaat tattatgtga ttgaagtcaa tccgcgtgtt
    33781 tctcgttcat ctgcgttagc aagtaaggcg actggctatc cgatcgctaa gctagctgct
    33841 aaaatagcgc ttggattaac attagacgaa atgaaaaatc ctgttacagg aacgactttt
    33901 gcagaatttg aacctgcatt agattatgtt gtagctaaaa ttcctcgctg gccgtttgat
    33961 aagtttgaaa acggagaacg gcttttaggc acgcagatga aagcaactgg tgaagtgatg
    34021 gctatcggtc ggaatatcga agagtcttta ttgaaagctg tgcgttcttt agaaatcggt
    34081 acacaccata tcgaacttcc tgagttggca gacatcacgg acgatgacct aatccaaaaa
    34141 gtggttcgag cacaagatga tcgcctgttt tatgtagctg aagctattag aagagggtac
    34201 accatagaag aattagcgga tttgacaaaa atcgatttat tctttttaga taaactgttg
    34261 catatcgtag aaatcgaaca gcagttgaaa cagcagccgt tggatgacaa gatattgaaa
    34321 acagcgaaac aaaacggttt tacagacaaa aaagtggcgg agctgtggga aataaacgaa
    34381 gaagccgttc gccagatgag aaaagaaaat gatttgattc ctgtatataa gatggttgac
    34441 acttgtgcag cggaattcga atcaaaaaca ccttattttt atagttccta tgaatatgaa
    34501 aatgaaagtt cacgatcaga aaagccctca attctagtac tgggatctgg tcctatccgt
    34561 atcggacaag gggttgaatt tgactatgcg actgtgcatt ctgtcaaagc aatccagcag
    34621 gcaggatacg aagcaatcat catgaacagc aatcctgaaa cagtttcgac tgacttttct
    34681 atttcggata aattatattt tgagccattg accttcgaag atgtgatgaa tgtgatcgag
    34741 ctagaacagc cgataggagt aatcgttcaa tttggtggtc agacagcaat caacttagcc
    34801 gaacctttga ctgctgctgg tgtaaaaatc ttagggacaa cgatcgaaga cttggatcgt
    34861 gcggaaaatc gtgatttatt cgagcaagcg ctgaaagagt tggatattcc tcaaccttta
    34921 ggggatactg ctacaagtaa agaagaagcc gtggttatcg cttcgagaat tggttatcca
    34981 gttcttgttc gtccaagcta tgtattaggc ggacgtgcga tggaaatcgt tgaaaaccag
    35041 cgagatttag aagattacat ggaacatgct gtgaaagcat ctccagaaca tccagtacta
    35101 gtggaccgtt acttgcttgg tggtgaatgc gaagtggatg cgatttgtga cggtgaaaca
    35161 gtactcatcc cagggatcat ggagcatatt gagcgtgctg gtgtccattc aggggattca
    35221 atggctgtgt atccacctca aacattatct gaatcgatca agaaaacgat cgaagattac
    35281 acgatccgct tagcattagg tttgaattgt gtcgggatga tgaatatcca gtttgtcatc
    35341 catgaaaatg aggtttatgt gatcgaagtc aatcctcgag caagtcggac tgttccattc
    35401 ttaagtaagg tgacaggaat tccaatggca caaattgcga cgaaagctat tttgggagag
    35461 aaactaaaga cacttggtta tgaaaacgga ctttatccag aaacaaaaga agttcatgtt
    35521 aaagcacctg tcttttcttt tacgaaattg cagaaagttg atacttactt agggcctgaa
    35581 atgaaatcga ctggagaagt aatgggatct gacagacaat tagacaaagc tttgtacaag
    35641 gcattcgaag cttctggatt acacttgcct gaatatggag ccgtattatt tacgattgcg
    35701 gacgaaacaa aagaagaagc actagaatta gctaaacgtt ttacagaaat tggctatagt
    35761 ctgataggaa cgaaacatac agctgcttat ttcgaaaaaa atggtctggt cgttgaaaca
    35821 gtagcgaaaa tttctgagga agcagcagaa aaaaatgtag tcgacttaat aagagatggt
    35881 aaaacacaag tggttgtcaa tacgattgat aaagatcggg gaaatgcttc taaagacggt
    35941 ttcatcatcc gaagagaagc agttgaacat ggtattccat tgtttacttc tttagacaca
    36001 gcagatgcga tcgttcgagt gatggaatcc cgtgcttttt caactaaccc tatttaacgg
    36061 cgagcacatg ttaagacaaa aagacgaaaa accgttgcag gaggggacag atgaaacaag
    36121 aaatgatgga aatcgttcag caaaaacagc tggcaccaaa gatttttgaa atgacattga
    36181 aaggtgatct agtaaaagaa atgcagcaac caggacagtt cttgcatatt ctcgtgccgc
    36241 gtgcagactt gctattacgc cgaccgatta gcttgaatca tatcgaccac gaaacaaata
    36301 cttgccgtat catttatcgg gtggaaggtg aggggactag agtcttctct actctttctg
    36361 ccggagacca tttagatgtc atgggtcctt taggcaatgg ttttgatttg tccaatcttc
    36421 atacaggaga cgaagtattt ataatcggtg gaggaatcgg tgtccctcct ctatatgagt
    36481 tatcaaaaca gttagtcgaa aaaggaatta agccaacgca ttttttaggt tttgccacac
    36541 atgaagtcgt ttattatgaa aaagaattta atcgtctagg agaaacaaga atcgctactg
    36601 acgatggaac atacggtatg cacggaaatg tcggtaattt attgttagga acgaagacgg
    36661 aacctacagc tgtttttgca tgtggttcaa acgggttatt gaagacagtg gaacagctgt
    36721 tttcttcgca tcaaaatgtc cagttatcat tagaatcccg catggcatgt gggatagggg
    36781 cttgctatgc ttgcgtttgt catacgccgg aagatgaaac aggaacaaaa agtgtgaaag
    36841 tttgtgacga aggaccgatt tttaaaatag gagaggtaat tatatgaatg agaatccttt
    36901 agcggtcaaa cttcctggcc tagaattaaa aaatcctatc atgccggcaa gcggctgttt
    36961 cggttttggc aaagaatacg gaaaatacta tgatttgaat cagcttggat cgatcatggt
    37021 caaggcaacg actccaaatg cccgctttgg caatcccacg ccaagagtgg cggaaacacc
    37081 atctggcatg ttgaatgcaa ttggtttgca aaatcctggt ctagacgttg ttatgcataa
    37141 acttcttcca gaattagagg agtacaatga gttacccatc atcgcaaatg ttgctggtgc
    37201 ttgtgaagaa gactatgtag aagtttgtag caaaattggc gaagcaccaa atgtgaaagc
    37261 aatcgagctg aacatttctt gcccaaatgt caaacatggc ggaattgctt ttggaacaga
    37321 ttctgatgtt gcttttcaat taacacaagc agtaaagaaa gtttcttctg ttcctgtcta
    37381 tgtcaaattg tccccaaatg taacagacat cgttccaatc gctcaggcaa tcgaagctgg
    37441 aggggcagat gggtttacga tgatcaatac tcttcttggt atgcggattg accttaagac
    37501 aagaaaaccg attttagcca atcaaacagg tggactctcc ggaccagcaa tcaagcctgt
    37561 cgctattcgt ttgattcacc aagtagcaag tatatccagc cttccaatca ttgggatggg
    37621 cggcgtgcag acagtggatg atgttcttga gatgtttatg gctggagcaa gtgctgtagc
    37681 tatagggacc gcgaatttca ccgatccgta tatctgtcct aaactaatta aagagctccc
    37741 tgtgcgaatg agcgaactag gcatcgaatc gctggaaaga ttaatcaaag aagtgagaga
    37801 ggaacgtgaa cgatgaatca actagaccaa cgaccaatta ttgcattaga tttttcaact
    37861 aggcaagagg tagaagactt tcttcgattt ttcccgaaag aagaaaaatt gtttgtgaaa
    37921 atcggaatgg aattgttcta ccaagaagga cctgaaatcg tacgttatct aaaagaggct
    37981 ggtcataatg tatttttaga tctgaaactt cacgatattc cgaatacggt cgaaaaagca
    38041 atgcggggat tggcaaagct tggtgttgat ctcacctgtg ttcatgccgc tggcggtatt
    38101 cgtatgatgg aggcggcaat gcgaggatta gaagagggga ctccagaagg tggaaaacgt
    38161 ccgctacttc tagctatcac tcagcttact tctacaagtg aagaagaaat gcatgccgat
    38221 caactaatcg aagtttcttt agaaaaaagt gtgatccatt atgctagctg tgccaaaaaa
    38281 gcaggattag atggtgtggt ttcttctgct tgggaagtag aagcgatcaa agaaatggca
    38341 ggagacgaat ttgtttgctt aactccgggt ataagaccag aaggaacagt cgcaggagat
    38401 cagacaagag tagtcacacc ttctcaggca aaaaagatcg gctcaacttt tatcgttata
    38461 ggtcgaccaa tcactcagtc aactaatcct tataaagcat atcaaacgat tcaaacagaa
    38521 tggagctaac ctaaaatgaa tgttgaacaa agtatcgcaa aagacttatt agaaattgaa
    38581 gcagtatttt taaatccaag taatcctttt acatgggcaa gtggaatcaa aagcccaatt
    38641 tactgtgata accgaatcac gatgagctat ccaaaagtcc gaaaagagat cgcaaaagga
    38701 ttagcaagca aaatcaaaga agcttaccct gaagtacaag tgattgctgg aactgcaaca
    38761 gcaggaatcc ctcatgccgc atgggtagcg gaaatactgg atttgccaat ggtctatatt
    38821 cgaagtaagg cgaaagatca tgggaaaggt aaccaaatcg aaggacgtat atttgaaggt
    38881 cagaagatgg ttgtgatcga ggatttgatt tctacaggcg gaagtgtctt ggaagcagct
    38941 gaagcagcaa aaagagaagg tgcggatatc cttggtgttg cggctatctt tacgtatgag
    39001 ttgccaaaag gaaaagcaaa tttcgaaaaa gccgaaatcc ctcttatgac gttgaccaat
    39061 tattctgtat tgattgaagc agctttagaa gatcgttata tcgatgagca agaattaact
    39121 ctgctaaaag aatggaaaaa agatcctgaa aattggcaga tttaagcgcg cttgatgaag
    39181 atagagaaat ccgatttgtg tctccagaat catcaattgg aatagcaaaa atccaaaaac
    39241 tgattgcgag aagcatcttt tcatcaggta tgaaaagaaa cttctatcgt gatcagtttt
    39301 tgattttttt aaaattcatt tttctaagac tggttagcag aaagaagaga ataatctctt
    39361 ctcttttttc tattatatat agattttctc tattttttgg taagggagct gtttcaaaaa
    39421 tggtaaagag cggaaatgtt gcgaatatca gttatttaat gatatgatat tcggaaaaag
    39481 aaatctcaaa gggaggtaac ttttatgggg ttgaatgaat ttattttagg attgaacaat
    39541 ttagagacga tcacaagggc acctggattt tttaaatata cagagcatac agttgcagca
    39601 cattcgtatc gtgtagcatc tattgcgcaa gttctaggag acattgaaga agcgaatgga
    39661 caaccgatcg attggaaact gctctatgaa aaatcattga atcatgacta cacagagcgt
    39721 tttattggtg atatcaaaac gccggtgaaa tacgcaagca aagagttaag agggatgctg
    39781 caaacagttg aagaaaagat gacggacgaa ttcatcaacc aagagattcc agctgagttc
    39841 caagaagttt atcgccgtcg gttgttcgaa ggaaaagatg aaaccgtaga aggagaacta
    39901 ttagccattt cagataaggt tgaccttctt tatgaatctt ttgatgaaat catcaaaaat
    39961 aacccggaaa aagtttataa ggagatgttc attgaggcag tgactacgat taaaagcttt
    40021 gaacatcggc cttctgttca gtatttcttt aaagagatat ttccggaact attgaaccag
    40081 gaattttatg gaaaagaggc gtttttagaa accgtctcaa cgattgtaaa gaaagaagaa
    40141 aaataaaaat gatatgagat gcagggggaa gtatagttga aaaagaatat ggatgtcgaa
    40201 tggggctatt ctggagacaa aggtccggaa aattgggcga gtttatgtga ttggtttgcg
    40261 agaggtgcgg aatttccatt gcagtcgcct attcatttga ccaaggggga agaaaccaaa
    40321 atagaaggag acacgctttc ctttcattat caaaagcaac ggtttactga caaggaattc
    40381 aaaaacacga ttcatttagt accttttgac caactcagtt atgtagagtt caagaaagaa
    40441 cgctattatt tgacagatat tcatttccac ttgccttctg aacatatcat caacggaaaa
    40501 caggaagata ttgaatttca ttttgttcat atgaacagca aaaaagagaa tctagttgtg
    40561 ggtgtcatgt accagctaat ggtacaggaa ggttggctgt ataatcaaga aaatggggaa
    40621 agttggaact tagaaaaaca tgagcattgg gtaaatccgg atgtgttctt tccggagcaa
    40681 aaaagccatt tccattatat tggctcattg acaactccgc ctaccagcgg gccaatccaa
    40741 tggtttgtaa tggatcatgt aggaaaactg aaccaagagt ttcttctccc cttcgatggt
    40801 cagtatgctc gaccgaacaa ccgaccaatc cagcccttga atggaagaga aatttattat
    40861 ttcgaggagt ttgagcaatg aatattcagc agatgaaata cgtggtcgct gtagcaaaca
    40921 atggtagttt cagagaagcg gcaaagaaaa tgtttgtctc ccagccaagt ctttcagctg
    40981 gtatcaaaga attagaaacc gagttaggga tcacgctgtt tactcgaacg aatcgagggg
    41041 catatttaac agaagaaggg caagacttct taaatcgtgc cgagaaaata ttggtccagc
    41101 ttgatacatt agaaactcac tatctaacga gtgaaaagag agacaatttt tcgatcgctg
    41161 cccagcatta tgatttctta ggtcctttga ccgcacagct actcgaggaa ttcaaagacg
    41221 agatcaaaca atttcggatc atggagacga caacagcgag agtgatcgaa gaagtcaaag
    41281 agcagcacag tgagatcggc attatttata tgaatgagca taatcaagct ggtatcagaa
    41341 gatatttaca acaaggagag ctaagtggcc gcagcattgg aaaattcaaa acgcatattt
    41401 ttttaaggca aggacatcca ttagctgaac acgcagagat caccaaagaa gaattgttat
    41461 cttatcctca agttcgtttt acacaggatg gaaataattt cccgtatttt tatgaagacc
    41521 ttatcgagat ccctgatcaa gaaacagtga tttatacgag tgatcgaggt actttgatga
    41581 atatcgttct tgaaactgat gcttacgctt caggatctgg tattgtgatt ggagggattc
    41641 gcgagcactt gcggttaatc cctttggcag aaggcgaact aaatgaattt tatatcattt
    41701 attcggcgaa acgtccgctc agtgaaatcg ctcaaagatt tatcaaagag cttactcggt
    41761 tactggacca gcagagctaa gaaaagcaga tgagaacgct gtttattgaa caccgttgat
    41821 caaaaaagag gggttgtgac agaattttgt cacaactcct ctttttgtag caaacaattt
    41881 ataactaatt gctgttgtgg gaaagtacgc aaatatgatt ctagaaacac tcattgatta
    41941 cctgttttgt ttcatagctg taattttttc ttcttctggt gtcacgataa tcgtcttttg
    42001 gttttcataa ataacataac caggcttggc gccatttggt tttcggatat gctttacctg
    42061 tacaagatca accggcactt gggcagagta tcggtatttt gaaaaatagg cagctaactc
    42121 ggcagcttct gtaattgttt catctgaggg cttgtcactt ttaatgatga cgtgcgaacc
    42181 gggtatattt tttgcatgaa gccaatagtc ggttttttta gctgtcttca ttgtcagctg
    42241 gtcattttga agattgtttt taccaacaag aatgagcgtt ccgtcagaag aaaagtattg
    42301 atcaggctga gatggtttct tacgtttttg tttttttaag ttcttctttt tcaggtatcc
    42361 ttctgaagtc aattcttctt taattgcttc gatatccatt gggccggcaa tttccagttg
    42421 agataagaca gattcaagat attggatttc atctttggct tcttggattt gttcgccaat
    42481 cagcttaaca gcgttcttca gcttttggta tcgatggaaa tatttctgtg cattttgatt
    42541 tggtgtcaat gccggatcta gtgctatttt gattggccga tcttcttcat aataattagg
    42601 taaaacaact tcttttgcgc cgcgtggtac ttgtgtcaga aaagtagtaa gtagttcgcc
    42661 atttctacgg taattctcag cgttttcgga ttctttgagt gtttgttcac gttttttcaa
    42721 tttattttta tttcgtttta gttcattttc gatttttcga atcagttctc cgccttgttg
    42781 ttttgcccga tctttctctg ctttttcgtg ataatagcta tctaataatt gactgagtgt
    42841 tggataagct gttacagcag atgcctgttc ggacaatacc tgataagcaa tcggtgtgaa
    42901 aaaatcttta ttttccgttt cgtaaaaagt aggaataggc tgatggctga tggctgagaa
    42961 aaattcgtcc catacaacca tcttttcatt aggacgttcc agtaatcttt tggtcagctc
    43021 ttgagcgata tcgaaaccga ttccctgaaa ctgacgttgg attgcttttg gatctagttc
    43081 agtttgggaa agccgctgaa agattttttc tttctcggag ctaaatgggt tcaagacctc
    43141 ttgttttggc ggagcaacat attctacacc aggaagcaaa gaacgataag tgttttgaga
    43201 gcttccaatg tgtttgatcg catccagtat ttttccagtt tctcgattaa ctaagacgat
    43261 cgtactatga cgtcccatta attcaacgat caatatgata ttttgtagat cacccaactc
    43321 gtttcgttta gcaaagtgga aatggatcac tcgatcattt tcgatttgtt cgatgtcttc
    43381 taaaatagcg ccatccaaat attttcgaag catcatcacg aagttaggcg gattatcagg
    43441 attctgataa tcgatttggg taatctgtac acgagcataa cttgggtgcg cagataataa
    43501 taatcgatgg tttttgccgc gtgaacgaat caccagcacg atttcatttt cataaggctg
    43561 atggattttc gagatccggc cagatagtaa cgtttcacgc aattcgttga ccatcgcgtg
    43621 ggtaaataca ccatcaaagg acatggttca ttcctcattt cttttcataa gtatattcat
    43681 tataacgaaa aaaagaagaa aaaccaattt ggccgaatag taaagcaaaa gtctagaaaa
    43741 atcttggact aattacataa aaaacagtgt ttctatcgtt gaaatccaaa tttttaggta
    43801 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
    43861 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
    43921 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
    43981 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
    44041 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
    44101 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
    44161 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
    44221 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
    44281 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
    44341 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
    44401 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
    44461 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
    44521 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
    44581 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
    44641 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
    44701 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
    44761 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
    44821 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
    44881 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
    44941 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
    45001 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
    45061 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
    45121 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
    45181 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
    45241 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
    45301 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
    45361 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
    45421 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
    45481 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
    45541 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
    45601 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
    45661 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
    45721 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
    45781 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
    45841 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
    45901 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
    45961 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
    46021 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
    46081 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
    46141 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
    46201 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
    46261 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
    46321 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
    46381 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
    46441 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
    46501 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
    46561 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
    46621 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
    46681 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
    46741 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
    46801 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
    46861 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
    46921 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
    46981 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
    47041 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
    47101 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
    47161 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
    47221 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
    47281 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
    47341 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
    47401 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
    47461 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
    47521 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
    47581 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
    47641 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
    47701 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
    47761 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
    47821 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
    47881 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
    47941 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
    48001 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
    48061 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
    48121 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
    48181 gtttgattgg ctataatact gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
    48241 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
    48301 ctgttcaagc agcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
    48361 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
    48421 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
    48481 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
    48541 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
    48601 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
    48661 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
    48721 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
    48781 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
    48841 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
    48901 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
    48961 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
    49021 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
    49081 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
    49141 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
    49201 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
    49261 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
    49321 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
    49381 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
    49441 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
    49501 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
    49561 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
    49621 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
    49681 atctggatgg tgtagatatt aaaaaagggg gccagaagat ggatggacat gttcttttgc
    49741 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
    49801 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
    49861 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
    49921 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
    49981 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
    50041 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
    50101 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
    50161 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
    50221 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
    50281 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
    50341 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
    50401 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
    50461 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
    50521 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
    50581 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
    50641 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
    50701 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
    50761 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
    50821 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
    50881 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
    50941 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
    51001 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
    51061 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
    51121 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
    51181 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
    51241 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
    51301 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
    51361 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
    51421 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
    51481 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
    51541 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
    51601 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
    51661 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
    51721 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
    51781 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
    51841 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
    51901 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
    51961 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
    52021 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
    52081 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
    52141 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
    52201 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
    52261 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
    52321 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
    52381 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
    52441 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
    52501 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
    52561 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
    52621 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
    52681 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
    52741 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
    52801 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
    52861 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
    52921 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
    52981 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
    53041 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
    53101 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
    53161 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
    53221 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
    53281 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
    53341 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
    53401 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
    53461 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
    53521 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
    53581 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
    53641 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
    53701 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
    53761 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    53821 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    53881 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    53941 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    54001 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    54061 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    54121 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    54181 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    54241 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    54301 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    54361 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    54421 gaaatagtgg tttagaacta gtgggctgat tttttatata ttttagttta cataatatat
    54481 attatacaaa gtagatttaa aaa
//
LOCUS       DACEMO010000003        50317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEMO010000003 DACEMO010000000
VERSION     DACEMO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.003"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            533..3367
                     /locus_tag="IW728_000105"
     CDS             533..3367
                     /locus_tag="IW728_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1023493.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3558..4049
                     /locus_tag="IW728_000106"
     CDS             3558..4049
                     /locus_tag="IW728_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1023494.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            4152..5141
                     /gene="manX"
                     /locus_tag="IW728_000107"
     CDS             4152..5141
                     /gene="manX"
                     /locus_tag="IW728_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1023495.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            5174..5977
                     /locus_tag="IW728_000108"
     CDS             5174..5977
                     /locus_tag="IW728_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1023496.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5998..6912
                     /locus_tag="IW728_000109"
     CDS             5998..6912
                     /locus_tag="IW728_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1023497.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6995..7369
                     /locus_tag="IW728_000110"
     CDS             6995..7369
                     /locus_tag="IW728_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1023498.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7508..7753
                     /locus_tag="IW728_000111"
     CDS             7508..7753
                     /locus_tag="IW728_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1023499.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7925..8989
                     /locus_tag="IW728_000112"
     CDS             7925..8989
                     /locus_tag="IW728_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1023500.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9235..9660
                     /locus_tag="IW728_000113"
     CDS             9235..9660
                     /locus_tag="IW728_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1023501.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9653..10102
                     /gene="nusB"
                     /locus_tag="IW728_000114"
     CDS             9653..10102
                     /gene="nusB"
                     /locus_tag="IW728_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1023502.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10199..11053
                     /locus_tag="IW728_000115"
     CDS             10199..11053
                     /locus_tag="IW728_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1023503.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            11054..12391
                     /locus_tag="IW728_000116"
     CDS             11054..12391
                     /locus_tag="IW728_000116"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1023504.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12396..12620
                     /locus_tag="IW728_000117"
     CDS             12396..12620
                     /locus_tag="IW728_000117"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1023505.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12622..13512
                     /locus_tag="IW728_000118"
     CDS             12622..13512
                     /locus_tag="IW728_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1023506.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13521..14339
                     /locus_tag="IW728_000119"
     CDS             13521..14339
                     /locus_tag="IW728_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1023507.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14446..14895
                     /locus_tag="IW728_000120"
     CDS             14446..14895
                     /locus_tag="IW728_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1023508.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14913..16595
                     /gene="recN"
                     /locus_tag="IW728_000121"
     CDS             14913..16595
                     /gene="recN"
                     /locus_tag="IW728_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1023509.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16650..16766)
                     /locus_tag="IW728_000122"
     CDS             complement(16650..16766)
                     /locus_tag="IW728_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1023510.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16839..17795)
                     /locus_tag="IW728_000123"
     CDS             complement(16839..17795)
                     /locus_tag="IW728_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1023511.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17966..18334
                     /locus_tag="IW728_000124"
     CDS             17966..18334
                     /locus_tag="IW728_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1023512.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18504..18935
                     /gene="mraZ"
                     /locus_tag="IW728_000125"
     CDS             18504..18935
                     /gene="mraZ"
                     /locus_tag="IW728_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1023513.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18956..19915
                     /gene="rsmH"
                     /locus_tag="IW728_000126"
     CDS             18956..19915
                     /gene="rsmH"
                     /locus_tag="IW728_000126"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1023514.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19920..20321
                     /gene="ftsL"
                     /locus_tag="IW728_000127"
     CDS             19920..20321
                     /gene="ftsL"
                     /locus_tag="IW728_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1023515.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20322..22514
                     /locus_tag="IW728_000128"
     CDS             20322..22514
                     /locus_tag="IW728_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1023516.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22540..23502
                     /locus_tag="IW728_000129"
     CDS             22540..23502
                     /locus_tag="IW728_000129"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1023517.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23516..24877
                     /locus_tag="IW728_000130"
     CDS             23516..24877
                     /locus_tag="IW728_000130"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1023518.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24903..25991
                     /gene="murG"
                     /locus_tag="IW728_000131"
     CDS             24903..25991
                     /gene="murG"
                     /locus_tag="IW728_000131"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1023519.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            26006..27226
                     /locus_tag="IW728_000132"
     CDS             26006..27226
                     /locus_tag="IW728_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1023520.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27382..28707
                     /gene="ftsA"
                     /locus_tag="IW728_000133"
     CDS             27382..28707
                     /gene="ftsA"
                     /locus_tag="IW728_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1023521.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28730..29971
                     /gene="ftsZ"
                     /locus_tag="IW728_000134"
     CDS             28730..29971
                     /gene="ftsZ"
                     /locus_tag="IW728_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1023522.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29988..30665
                     /locus_tag="IW728_000135"
     CDS             29988..30665
                     /locus_tag="IW728_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1023523.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30679..31278
                     /locus_tag="IW728_000136"
     CDS             30679..31278
                     /locus_tag="IW728_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1023524.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31293..31580
                     /locus_tag="IW728_000137"
     CDS             31293..31580
                     /locus_tag="IW728_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1023525.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31629..32411
                     /locus_tag="IW728_000138"
     CDS             31629..32411
                     /locus_tag="IW728_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1023526.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32432..33139
                     /locus_tag="IW728_000139"
     CDS             32432..33139
                     /locus_tag="IW728_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1023527.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33401..36199
                     /gene="ileS"
                     /locus_tag="IW728_000140"
     CDS             33401..36199
                     /gene="ileS"
                     /locus_tag="IW728_000140"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1023528.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36248..37774)
                     /locus_tag="IW728_000141"
     CDS             complement(36248..37774)
                     /locus_tag="IW728_000141"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1023529.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37789..38436)
                     /locus_tag="IW728_000142"
     CDS             complement(37789..38436)
                     /locus_tag="IW728_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1023530.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38620..38949)
                     /locus_tag="IW728_000143"
     CDS             complement(38620..38949)
                     /locus_tag="IW728_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1023531.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            39126..39854
                     /gene="sfsA"
                     /locus_tag="IW728_000144"
     CDS             39126..39854
                     /gene="sfsA"
                     /locus_tag="IW728_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1023532.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39870..40883
                     /locus_tag="IW728_000145"
     CDS             39870..40883
                     /locus_tag="IW728_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1023533.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40883..42160
                     /locus_tag="IW728_000146"
     CDS             40883..42160
                     /locus_tag="IW728_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1023534.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42223..44925
                     /locus_tag="IW728_000147"
     CDS             42223..44925
                     /locus_tag="IW728_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1023535.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            45077..45394
                     /locus_tag="IW728_000148"
     CDS             45077..45394
                     /locus_tag="IW728_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1023536.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45424..45744
                     /locus_tag="IW728_000149"
     CDS             45424..45744
                     /locus_tag="IW728_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1023537.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45852..47312
                     /locus_tag="IW728_000150"
     CDS             45852..47312
                     /locus_tag="IW728_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1023538.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47380..47601
                     /locus_tag="IW728_000151"
     CDS             47380..47601
                     /locus_tag="IW728_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023539.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47632..47814
                     /locus_tag="IW728_000152"
     CDS             47632..47814
                     /locus_tag="IW728_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1023540.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47814..48227
                     /locus_tag="IW728_000153"
     CDS             47814..48227
                     /locus_tag="IW728_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1023541.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48350..49531
                     /locus_tag="IW728_000154"
     CDS             48350..49531
                     /locus_tag="IW728_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1023542.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            49920..49993
                     /locus_tag="IW728_000155"
     tRNA            49920..49993
                     /locus_tag="IW728_000155"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49954..49956,aa:Arg,seq:cct)
     gene            complement(50062..>50317)
                     /locus_tag="IW728_000156"
     CDS             complement(50062..>50317)
                     /locus_tag="IW728_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1023543.1"
                     /translation="VSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNILSVSKR
                     LGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        16744 a         8503 c        10846 g        14224 t
ORIGIN      
        1 tagaaaattc caagatattt aataatagaa gaaaagcact aacatcatgg gttcagaaga
       61 attgagagct tatcaggaaa cagtataatt gaagtgtctg tttatccagt agctcaagaa
      121 gcttaattga atcttatgat taaagaggtt gtgacaagac ttttgtcaca gccttttttg
      181 tttataaaaa taaaagttgt agtggaggct acgaagttta ctttgtcttt ttacacttaa
      241 tatttttttg ccaatttgga atcaggatgt tttgataact atcgttttca attgatggaa
      301 gctatattgt ttgctatcag tgaaataatt atatggtaaa cagtgttctt tttcttgttt
      361 ccattataaa aatcgtatcg ggatttttcg ttcttttaaa ttgaacattt ctatctatcc
      421 gcgtgttatc aacaaaacaa aatcaggtaa atagttgacg aaacagaaat aaatacatag
      481 tattatgttt tgaagcctaa gtacaatagt ctatagaaaa ggagcgatac cattggcaaa
      541 acgcattgac cgaatttata tgtatgtcaa agaaaaaacc gctcatttaa caaaggcaga
      601 atatgatcaa ggagtaacaa cacaggaaat tgctgaagtg ttagggattc agcgaacaaa
      661 tagcagtaaa gatctgaatc agctagtgcg cgaaggaaaa ctgataaaga cagacggacg
      721 accagtacgt tacatatatc aaacactcgt tacgcacgta aagccagtta caaaacatgt
      781 agtcagttac aaagaaacgc cggtctctga agaaaaaaat acactccaaa tagatactaa
      841 ggatatcttt gcaaaaatca ttggagctaa cggcagcatg aaaaattcag tggaacaggc
      901 aaaagctgcg attttgtatc cgccaagagg attaaattgt ttgattacag gacctaccgg
      961 atcaggaaag acttattttg cacacgcgat gtatcatttt gcaaaagcgc atcatgtaat
     1021 agatgaggaa acggaactga ttgttttcaa ttgtgctgat tatgccaata accctgagct
     1081 tttgatgagt catttatttg ggtacgcaaa aggtgctttt acaggcgcag atgaagaaaa
     1141 aatggggatt atcgatcaag cggatggcgg tatgctgttt ttggatgaga ttcaccggtt
     1201 gccgcctgaa ggtcaagaaa tgatctttta ctttatggat catggaactt acagtcgtct
     1261 tggggaaaca acgaaatccc atgaagcaaa tgtacggatc gtaggtgcga ccacagaaga
     1321 cccaggttct tctctattag aaacttttgt tcggcgtatc ccaatcaata tcaaacttcc
     1381 ggcttttgaa aaacgtcctg caaatgagaa agtcgattta gtaaaaatca tgattgccca
     1441 cgaagcaaac cgcacgcagc gaaagatttc tttgacagaa gatgtagcaa aagcattgat
     1501 cggcagtgtg acctatggaa atataggaca attgaagtca aatatccagt tagtatgtgc
     1561 acgaggattt ctaaatcata tgcaatcacc cgaaatctcg atcacaatcg atgatttgac
     1621 agaagggatt cgtagcggat tgattcaatt agcaagcaac cgcagtgcaa tggcagagct
     1681 ttctaaaata ttggagccaa agataactgt agcaccaaat gatacgatca tgaaaatcca
     1741 atcggactcc tatgagttac cttataatct atatgatatc atcggagata aagcagcttt
     1801 actgaagtcg gatggtttgg atcaagaagc aatcaatcat ttcatttcta cagatatcaa
     1861 catccatctg aagtctttct ataaagacca tggtttttct tttaatgcag acagcaaatt
     1921 agcagaattt gtagatccga aaatcataga agtgaccaat cagatttatg taatggtcaa
     1981 aagatcttta ccatatgagt ttcagcaaaa ctttatttat gctatgagct tgcatatcag
     2041 ttcttttttg aaaaaaatca acttaggaga agaacggcat acgaatgata acatccgtga
     2101 gatggctttt gattttccag atgaatatgc tgttgcgaaa gaagtgcgta gttatatcga
     2161 aacgtatttt caagtggaga ttccagaaag tgaagactat tacttgaccg ttctactcgt
     2221 ctcgttacgg gcaaatcaag caagcggcag aatcggtgta gtcgttgcag ctcacggaaa
     2281 cagcacagca agcagtatgg tccaagttgt ccaacagttg ttagatgcag agaacgtacg
     2341 agcagtagac atgcctttgg atatggatcc gaaaacagca ttggaccgaa ttgaacgcaa
     2401 tgtacaagaa gtagatgaag gaagcggggt catactctta gtagatatgg ggtcccttgc
     2461 gtcgtttaat tcgcaaattc aaagagacac tggaattcca gtacgtactg ttgatatggt
     2521 aacaacttct ctagttttgg aaactgtacg aaaggtctca gtattaggga cagatctaga
     2581 tatgttgcat gaatcattga aaaattttcg aggttacgca gaaatatctt ctgtagaatc
     2641 tgttcaggaa gcaaaaccta ttcataagaa aaaagctgtt ttggctatct gcgcatctgg
     2701 cgaaggtaca gcacaaagaa tcaaagaatt gatcgaacga gcggtaaaca agcgtcaaga
     2761 aacagaatta gaagttctag ctttgtcgat tgtggaagtg aaagaagaac tgccagttat
     2821 ccaaaaagaa tatcagatca tagctactac tgggattact gatttgaata tccaagcccc
     2881 atttattcct ttagaacgtt ttattgatca aaatatcgaa gtcattttgg atcagttgct
     2941 gatggaatca gaattggagg aaactgaatt catctcccta gatgaagagt cagctaaaaa
     3001 tacgtgtgtt gagtttatct ctgataactt catctttatt aatggtagta agttaatcga
     3061 tccaatgtgg caattttcta cacaaatctc tcagacgacg ggaatcggcg atgaagaata
     3121 tggattcaaa atcaacctag tgatgcacac agcaggaatg atcgaacgta ttattcggaa
     3181 tgaaccattg actgtggaag aaaacgaatt gacaaataca acgaatgatc ctctttatag
     3241 ccaattggca gcctcagttg ttctgttgga agatcagata aaagtaaaag tgccgataga
     3301 agaaatgtat tatctgctac gtctagtcca taatcagtta gataaaaaag aatacactgt
     3361 gccatgaata cacaaaaacg atacactttt atagtgtatc gtttttgtgt attggttaag
     3421 aatcctttaa attcaattag tgtatcgtaa aaaagttggc acgctttttg cttatagtag
     3481 tgtgtgctag gaggtggttg ttcacacaga aatgataggg gtttctgtga agtagtgagg
     3541 aagaaggagt tttgcagatg gatattcgat tggcacgtat agatgatcgt ttaatccacg
     3601 ggcaagtcgc aacagtttgg tcgaagcaaa caggtgttga acgtatcatt gtgatcagcg
     3661 atgtagtcgc tcaagataaa ttgagaaagt ttctactaaa agaagctgca ccgccgggga
     3721 tcacagcaaa tgtaataacc gtgcagaaaa tgattcaaat ctatcatagt ggattattat
     3781 cacgagcaaa agtgatgctt ttattcacca atccccaaga tgttgaagcg gttgtccgct
     3841 gcggtgtaca gttgaagtcg ttgaatatcg gcggtatgag ttttactgaa ggcaaatcaa
     3901 tgattaccaa ttttgtttca gtaaatcaac aagatattga ttcctttcgt tacttgaaca
     3961 gtcaagaagt ggagttagag attcgaaaag tacctgctga caggaaaatc cttttgatgg
     4021 aactactagc gaaaatcaaa aaggtgtagc aagaatggtt gttggaaaag gtcaaataat
     4081 tctgttgtaa tggttagcaa ttattaatat ttttgaattt gttacaacaa aagtaaatag
     4141 gaggtataaa tatggtaggg attatcctag caagtcatgg acaatttgct gaaggaatct
     4201 tacaatccgg ttcaatgatc tttggcgaac aagaaaacgt caaagccgtg attttgaaac
     4261 caagtgaagg tccagatgac ttgagagcca aattggaaga agcggttgct tcttttgata
     4321 atcaagacga agtgttattc ttagttgatt tatggggcgg aacaccgttc aatcaatcaa
     4381 acacattatt cgaagagcac aaagataaat gggcaatcgt cagcggtttg aatttaccaa
     4441 tgttgattga agcctacgca tcacgctttt caatggaatc tgctcatgaa attgcagcac
     4501 acatcattga aacagcaaaa gacggtgtaa aagtaaaacc ggaagaatta gaaccggcag
     4561 aagcaccgaa agcagcagtt gaagacgcac aacctaaagg agcacttcca gaaggaactg
     4621 tggtcggaga cggaaaaatc aaatatgtat tagcacgtgt tgactcacgt ctgttacatg
     4681 gacaagttgc gacagcttgg acaaaagcag tgcaaccaaa ccgtattatc gttgtttcag
     4741 atgcagtatc aaaggatgat ctgcgtaaga gactaatcga acaagctgct cctccaggtg
     4801 taaaagcaaa tgttattcct attagcaaaa tgatcgaagt agcaaaagac cctcgttttg
     4861 gaaataccaa agcattgctt ttatttgaaa atcctgaaga cgtattgaca gctgttgaag
     4921 gtggcgtaga catcaaagaa ttaaatgttg ggtcaatggc gcattcggtc ggtaaagtcg
     4981 ttgtaagtaa agtattgtct atgggacaag aagacgttga agcgtttgag aagcttgaac
     5041 aaaaaggtgt gaaattcgat gtacgtaaag tacctaatga ttcacgcgat aacatggatg
     5101 atattttgaa aaaagcaaaa gctgaattag caaaagcgta ataaataata ttaaaattta
     5161 taggaggctt atcatgtcta ttatttcaat aattttagtc gtattagttg cctttctagc
     5221 tggtatggaa ggtattcttg atgaatttca atttcatcaa ccactagtgg catgtacatt
     5281 gatcggttta gttaccggta acctcgaagc tggtatcgta cttggcggta cgcttcagat
     5341 gatcgcttta ggttgggcaa atatcggtgc cgctgtagca ccagatgctg cattagcatc
     5401 agtagcatca gcaattattt tagttttagg tggacaaggt gttaaaggtg ttccttctgc
     5461 gatcgcgatt gccgttcctt tggctgttgc agggttgttc ttaacaatga tcgtacgtac
     5521 aatcgctgtt ccaatcgttc atttgatgga tgcggctgct gaagaaggaa acatccgtaa
     5581 ggttgaaatg tggcacatca ttgctgtatg tttacaaggg gttcgtatcg cggtcccagc
     5641 agcagcgttg ctatttatcc cagcacaaac tgtgcaatca ttccttgaat caatgccagc
     5701 atggttaaca gatggtatgg ctatcggtgg gggaatggtc gtagctgtag gttacgcttt
     5761 ggtaatcaac atgatggcta caaaagaagt atggccattc ttcgttattg gtttcgttgt
     5821 agctgcaatt tctcaattaa cattgattgc tctaggtgct ttaggtgttg cattagctct
     5881 tatttacttg aacctttcta aaatgggtgg ttcatcaaat aatggaggcg gaagcaactc
     5941 tggtgaccct ttaggcgaca ttttgaatga ctattaatct tgaaggagga aaataaaatg
     6001 gcagaagaaa aaatcaaatt atcaaaaaaa gatcgtttag ctgttgcgtg gcgctctacc
     6061 ttcattcaag gttcatggaa ctacgaacgt atgcaaaacg gtggctgggc attttctatg
     6121 attccagcaa tcaaaaaatt atacaaaaca aaagaagaac gttctgcagc attaaaacgt
     6181 cacttggaat tctttaatac acacccatac attgcttctc caattttagg ggttacattg
     6241 gctctggaag aagaacgtgc gaatggcgcg cctgttgatg atgtagcgat tcaaggggtt
     6301 aaagtaggta tgatgggtcc tttagctggt gttggtgacc cagtgttctg gtttacagta
     6361 cgtccaatgc taggtgcact aggtgcttca ttagctatgg gaggaaatat ccttggacca
     6421 atcatcttct tcttaggttg gaacattatt cgttgggcat tcatgtggta tacacaagaa
     6481 tttggctaca aagctggttc taaaattact gatgaccttt ctggcggatt gttacaagac
     6541 gtaacaaaag gtgcttcaat tttagggatg ttcgtattag ctgcccttgt acaaagatgg
     6601 gtatcaatca aattcttacc agttgtatca actgtaaaat tagataaagg tgcttacatc
     6661 gaatgggata aattgccttc aggtggtgaa ggaatcaaga gtgcatttga acaagttaat
     6721 aatggcttag ccctttctcc tgaaaaggta acaacattac aagacaactt agaccaattg
     6781 attcctggtt tagctgcatt aggattaaca ttgttatgta tgtggttgct taagaaaaag
     6841 gtttctccaa tcgttatcat ccttggttta ttcgtagtcg gtgttgttgg acacgtcatc
     6901 ggacttttat aatccataaa aatatagctc gggcgttgtc ccgagctttt tttatatagt
     6961 atactgttca taaacaaata tctggaggga gaaaatggtt caatcaatca atacaaaagt
     7021 agacttggta attgatgcta cttcgtttac gggtctgaca gattacgggc aaatcatgat
     7081 aggtgataat ggttttgagt tttaccatgc gcgagataga cgcaaatata tccaaattcc
     7141 atgggaagaa gtggattatg tactagcatc agtcatgcta aagggaaaat ggattcctcg
     7201 atatgccatt caaacgaaaa aaaatggtac atacacattt tcttccaaag aacctaaaaa
     7261 agtgcttcgt gcgatcagag aatatgtaga tgaagataga atggtacaat cattgagttt
     7321 ctttgatgta gtgaaacgtg gtattcgtag tatgtttaaa cgcaagtaaa aaataaagaa
     7381 aacttctaag aggaggtttt ctttattttt ttactttgca gaagaaaata tgaaatagca
     7441 aaaccaatta tttgtcccct ttaaatccca tatgctaaaa ttgattagca aagaaggagg
     7501 ttatattatg agttggttat ggacattgat tgttggagcc gtgatcggag caattgcagg
     7561 tgcgatcacc agtaaaggaa attcgatggg ctgtgtaacg aatatcattg ctggactagt
     7621 cggttcaagt attggacaag cgattttcgg cgaatgggga ccaaaagctg caggtatggc
     7681 acttattcca tctattttag gcgcagtgat tttggtagca gttgtctcat ttttctttgg
     7741 aaaacgatca taagttttta caaaatagta aactaaaagg agttgcagcg agactgtgac
     7801 tcctttttgg ctttatttat ttaaaataaa aagaagtttt ccggaaattt ttatttaact
     7861 gtcgtgtacg aagaaaagtt tgctataatg gaaaaaagcg tgcaaagaaa gagggagttt
     7921 cattatgatg ctacgtgtag aaaaattgcg caaaaaaatg caagaagaaa atttggattc
     7981 atttttggtt accagtccgt acaatcttcg ttacttaacg aactttacag gtacgacagg
     8041 attagctgtt attactttgg aaaaagcatt ttttattact gattttcgtt ataccgagca
     8101 ggcggcagcg caggctcaag ggtttgaaat tattaaaaat gtcggaccta tttttgatga
     8161 ggtagcaaac ttagtacgca aagaagaact gcactcatta gcttttgagg aaacaacagt
     8221 ctcattttta gaatattcgg ttttagaaga aatcatcgat gcggaattag tacctgtttc
     8281 acagatgatc gaagaactca gagaagtgaa agatgaagaa gaaatcgcta ttatcgaaaa
     8341 agcatgtcat attgcggata tggcatatga tcacatcctg aaaatgatcc aaccgggcat
     8401 gactgagatt gaagtcgcaa atcaactaga cttttatatg cgttctcttg gcgcttcagg
     8461 tgtttctttt gatacgatcg ttgctagcgg attgcgttcg gctatgccgc atggtgtagc
     8521 aagcaaaaaa atcatcgaac aaggtgactt gatcacattg gactttggtt gctattacga
     8581 agggtacgtt tccgatatga cacgtacatt cgctattggt gatcctgggg aaaaattaaa
     8641 agaaatttat caaatagtct tagatgctca attagcagtt attgatgctg caaaaccagg
     8701 aatgactggt gtccaattag atgcagttgc tagagattat atttcaaaac atggttacgg
     8761 ggaagcattt ggacattcta caggacatgg gatcggttta gagatccacg aaggaccaaa
     8821 cgtttcttct cgtgcagaaa agcaatttgt accaggaaat gtgatcactg atgaaccagg
     8881 gatctactta ccaggaatcg gtggagtacg tattgaggat gacttgttga tcacagaaga
     8941 tggaaatcgt gtgttgactc gttcaccaaa agaattgatc atcttatagt ttcatcaata
     9001 gaagaagtaa gcgtaaaaat cagtgttcac agactttgtt atagaaaatc actcgattta
     9061 atgatacact atagaagaac ttttaagctg taagagataa taaagttggg tgttttctcc
     9121 aattcatagc agtttgcagc tgatcggcgg cagttttctt accacgtgat cactatgccg
     9181 catcatagag aattcggatg tatctcgtgc ttaggcaaag ggaggaataa ataaatggca
     9241 gatgaaaaaa atttgatttt aaacgcaaac caagatttag gagagatcgt gatcgctcct
     9301 gaagtcatcg aagtaattat cgggattgca gcttctaaag tagaaggcgt atacggaatg
     9361 cgcggaactt ttgccagcaa tgtcactgaa ttattaggac gtgcagctca cggaaaaggt
     9421 gtttacttag caaacgagga agatggcttg aaagtagatc tttactgtta tctagaatat
     9481 ggtgtttcag ttcctaaagt agcaatggaa atgcaggaac gggtcagcca gcaagtcctt
     9541 ttcatgacgg atatcgattt atctgaagtc aatatccatg tagtagctgt tgttcctgaa
     9601 aaattagaac aaccaacaat cgaagaactt tttgaagacg aagaggaaga gaatgaataa
     9661 agaattatca agacacgaga ttcgggaaat ggcattgcag gctttatttc cattagactt
     9721 caatgcagac ctaacaaaag aagatgcgat tttcaacgca atcgaattag accaccgcga
     9781 catgatcaac gaagatgaat cagagtttgt tcctgtttat cttgatacgc tagttggcgg
     9841 tgtctgtgca aaaaaagatg aattggataa agtgattgaa aaacatttaa aaaacaattg
     9901 gcacatcaat cggatctcaa aaatggattt ggtgatcttg cgtatcgcga tttttgaaat
     9961 gacttatgtg acagatgtac cagcggctgt tgctttaaat gaagcaatcg agttggcaaa
    10021 aacatttagt gatgaccgtt cacgtaaatt cgtcaacggt gttttatcca atgtattgaa
    10081 agaattagaa gctggggcgt aagtcctagc ttttttctat gaatcatcat aaacctgtta
    10141 taaatcggga aaattctatg gtagaatgaa aacacaaaaa atatctggga gtggttagat
    10201 ggcagaattg attaacggaa aagaattagc tgaaaaaatg caggcagaga ttgctgaaaa
    10261 aatccaaaaa ttgaaagata atggtatcca tcccggatta gtcgttttat tggtagggga
    10321 aaatccagcc agccaaatct atgtaagaaa taaagaacgt gtagcaaaag aaatcggaat
    10381 ccattcattg gtagagcgtt atcctgagac gatttctgaa gacgaactac ttgcagaaat
    10441 tgaaaaatac aatcaagatc ctagttttca tggtatattg gtccaattac cattgccaaa
    10501 gcatatagat gaagaaaaga tcctgttagc tatccgccct gaaaaagacg tagacggttt
    10561 tcatccgatg aatttaggca gactccttgc gggtaaaccg gacaagattc cttgtactcc
    10621 atacggcatc atgaaaatgt ttgaagctta ttctatcgat ccggcgggta aacgagcagt
    10681 agttatcgga cgaagcaata tcgtcggtaa accgatggct cagctactct tgatggcaga
    10741 tgcaactgtt actgttgctc attctaaaac cgaaaatctg gctgaactgg cacaagaagc
    10801 agatatttta gtcgtagcaa taggacgtgg acattttgtc acaaaagaat tcgtaaaacc
    10861 aggtgctgta gtgatagatg tagggatgaa tcgtgatgct aacggtaagc tgatcggtga
    10921 tgtgaaattt gatgaagtgg agcctattgc aggctggatc actcctgtgc caaagggtgt
    10981 agggccaatg actatcacga tgctgatgta tcaaaccgta aaatctgccg aagcttttgg
    11041 agcaggtgaa tagatggcac aagaatattt aactgtcact acattaacga agtatttgaa
    11101 aagaaaattt gatgccgatc cttatttaga acgcgtatat ttaacaggcg aaatatcgaa
    11161 tttccgttta cgtgccaatg cccatcaata tttcagctta aaagatgacc atgcaaaaat
    11221 atctgctatt atgttcaaag gagcattcca aaaattacga ttccagccaa aagaaggaat
    11281 gaaagtcctt gtcgttgggc gtatttcatt atatgaagcg ggaggttctt atcaaatcta
    11341 tgtagagcac atggagcctg atggaatagg tgctttatat caagaattgg aagaacgaaa
    11401 agaaaagctg atgaaggaag gcctttttca agggcctaaa aaaatgctgc cacgtttccc
    11461 aaagcgaatc gcggtcctta ccagtccaag cggagcggtt atccgcgata tcatcacgac
    11521 ggtgaaaaga agatatccaa ttgtccaatt ggttttgttc ccaacagttg tacaaggaaa
    11581 ccaagcagcg gacaatgttg tcaggaatat ccagagagta gaagagatgg gcaatttcga
    11641 tacaatgatc atcggtcgtg ggggtggttc gattgaggat ttatggccgt ttaatgaaga
    11701 gcgggttgct cgggcgattt atcaagctaa tacgccggtg atttcatctg taggacatga
    11761 aacagatacc acgatcgctg atttagtagc agatgttcgg gctgccactc caacagcagc
    11821 tgcagaatta gctgttcctg ttttgtcaga agagttgatg cgcattcaag aacggcaagc
    11881 tagattgcag caggcgtttg tacgccagat ccagcgcaaa caagaacggt tcgagcgtgc
    11941 tcgacgttcc tatgtatttc ggcagcctga aagattgtat gaaggtcaga ccatcaaatt
    12001 agaccagttg aaacagcgat tatatcaagc tacacagcaa atttatcatg cgaaagaaaa
    12061 acaaacgatc gcattaagcc accaattgga acaagtggct ccgctttatc gagttaaagc
    12121 agctcgccaa gaaactgctt atctagaaaa acgcttagaa gagaaaatcc aacaatatat
    12181 gaaacaacag cagcagcgtt ttgctcaagc tgttcaatct cttgatttgt tgagtccatt
    12241 gaaaatcatg ggaagaggct acagctatac gacgttaaat gatcaagtag taaaaagtgt
    12301 caccgagatt catcaagggg atttgctgac gatccattat caagatggaa tcgttaaagg
    12361 acaagtacaa gagatagaca aaggagaata aaaatatgga aaatccaact tttgaagagt
    12421 cactgcaaga attagaaaaa attgtcatgc agttagaaca aggagacgtt ccattagaat
    12481 cagctctaga ttcgtttaaa cgaggaatgg aattaagcaa acattgccaa gatacattag
    12541 ctaaagcaga agagaccttg acgaaaatga tgacagaatc aaatgaagaa gttgattttg
    12601 acggaaacga ggaaagctga gatgagatta actgactttt cagctattca tctacctgct
    12661 gtagaaaaag aaatcttgtc ttttttagat gagcatacaa cggatagagg actctatgat
    12721 gcgatgagtt attcagtgaa agcaggcggg aaaagaatac gtcccttatt acttttaacg
    12781 gccgttgctt cctttgatga gcctattgat gttcccgtct atcaagtagc agctgcttta
    12841 gaaatggtgc atacgtattc cttgatccac gatgatttgc ctgcgatgga caatgatgat
    12901 cttcgtagag gaaaacctac caatcataaa gtattcggcg aagcattagc tatattggcc
    12961 ggagatggtt tgcttacagg agcattccag ctaatcagta tggcacactt aggtaattct
    13021 cctaagcttc tgctgcttca gcaattagct gtttgtgcgg gatcacaagg tatggttgct
    13081 gggcaagctg cagatatcga gggcgaatcg aaaaagcttt ctttagaaga attagcattt
    13141 attcatgaaa gaaagacagg gcatttgatt cgttatgcct tgctggctgg tggcatatta
    13201 gcgaagcaac ctgaagaaat cttattgcta cttcaacgat tagccgaaca tttaggttta
    13261 gcttttcaga taagagatga tctactcgat gtgattggta caacgaaaac attagggaaa
    13321 acagctggta aagatgaacg tatggaaaaa aatacttacc cgcgtttatt aggcctagaa
    13381 aagacacgtg aggcgttgga gattgagctg ttatccgcca acaagatcat agacaaatta
    13441 gaagaggacg ttttttcttt tgatggcggg ctgatgcgcc agatgatcaa gcaatttgat
    13501 gtggagaaat gagggaaaga atggaaaaag aacgcgtaga tgtattagcg gtcaaacaag
    13561 gattatttga aacaagagaa caagccaaac gttcagtgat ggctggtttg atttacaacg
    13621 aaaaaaacga gcggtttgat aaaccgggag aaaaaattcc agtgagtagt gaattaagag
    13681 tcaaaggtaa aaaacttcct tatgtttctc gagggggatt aaaattagaa aaagcactca
    13741 aagcatttga tctatctgtt gaaggaaaaa cattgttaga tattggtgca tccactggcg
    13801 gatttactga tgcagctttg caaaatggcg caaaaatgag ttatgcttta gacgttggct
    13861 acaatcagct agcttggaaa ataagacaag atcctagagt cgtcgtaatg gaaagagtga
    13921 attttcgtta tgcaaaaccc gaagacttta ccttaggaat tccagaagtt gctgttatcg
    13981 atgtttcttt tatttcacta aaactgatgc tgcctccatt acatgctatt ttaaaagaag
    14041 agggagaagt gattgcttta atcaaacccc aattcgaagc aggcagagaa gctgtaggga
    14101 aaaacggaat tgttcgtgac cctcagaccc atcaaaaagt tttagaagac attttgtcat
    14161 ttgcggcaca aggtggatat gatgtgctgg aattaagtta ttctccgatc acggggggag
    14221 aaggaaatat cgagttcttg gctcacttga ggaaagtacc cgaaagcgga acaatcaatt
    14281 cagctattaa tatggcagaa gtggtttcaa atgcacacga acagtttgac cataaataaa
    14341 ttgtaactgt gaaaatcaag tgagaagtat gcggattctt ctttattata ccaaggaaat
    14401 aagctatcat tgattaagaa gaatatgcta aggagcgaag acgtaatgag aaaaaaagat
    14461 cggcatcgtt tgattactcg attgttgagt gagcaagata tccgaaagca ggaagaattt
    14521 gttgaaatcc tgaaaagcaa aggaatatct gttactcaag ctacgatttc cagagatata
    14581 aaggaactga aactgatcaa ggtaccggct tcagatggcg gctaccgtta tagcatacca
    14641 gctgaaacaa acgaagatgt aaatactaaa ttggaaaaac tattaaaaga cgctttcgtg
    14701 gcagttgacc aaatggaaaa atttgttatt ttgaaaacat tgccgggaaa tgcttcggca
    14761 gcagcaaatt tgattgataa acgttacaag aaagaattat tttcgattat caatgatgac
    14821 gataatgttt tgatgattac acgaactgaa gaccaggcag ttttattgaa gaaagacttc
    14881 ctgcattacc tttaagttga ggagcgaatg tgatgctaca agaaatcagt attactaact
    14941 ttgcgattat tccggaattg cggctttctt ttcacgaagg aatgaccgcg ctgaccggag
    15001 agactggtgc gggtaaatcc attattatcg atgctttagg tctgttagca ggaggaagag
    15061 gttcaagcga ttatatacgt caaggagcag aaaaatgtgt gcttgaaggg ttgtttgaac
    15121 taccaaaaca agaaggattc tctgaactga tggttgaatt agggatcgaa acagatgagg
    15181 acaatctgat tgttcgacgg gatatgtcct taactggaaa aaatgtttgt cgagtgaatg
    15241 gtcatattat cacattagct aatctgagaa gaatcggcag ttatttagta gatatccaag
    15301 gacaaaacga acatcaggaa ctgctccagc ctgaatcaca tcttgcttta ttggatcgtt
    15361 ttggagatgt agctttccaa cagaagaaaa agagttatca gcaagaatat atctcttatc
    15421 gtgagctgga aaaaagggta cgaaaagtgc agcaaaatga aaaatcctat gttcagagaa
    15481 tagatatgct ccatttccag caagaagaaa tagctgctgc tgatttgcaa gtcggagaag
    15541 aagagaaatt aaaagaagaa agagaaaaac ttagcaatta tcaaaaaatt gctgatggat
    15601 tagctgcagg ttacggagca ctgacgaata atgaacaaaa cagtgtagat ggtgttggct
    15661 tggccgtttc agaactgcaa gggatcgccc atctagatgt ggagtatgaa gcaatttatg
    15721 aaaatatcca aagtgcttac tatttgctgc aagatgcaat cggcgatatg agtcgtcaga
    15781 ttgatttatt ggagttagat gagagtcgtt tagaagaagt tacgcaacgt ttagaattaa
    15841 tccgacagtt gaaacgtaaa tacggcgaat ccattgagtc aatattggct tactatgacg
    15901 aaatcaccga agagctagct tcttctgatt tttcagaagg tcaattggac aaaatgaaag
    15961 aggaactaga gcaaaaagag cttctacttc agcaacaagc cgcagatctt catgaagcgc
    16021 gtaaaaaaat cgcaaaagaa ttggaaaaat ccattctgca tgaattgaaa agtctgtata
    16081 tggaaaacac tgagtttgag gtacgtttct taaaagaaga aaatagacaa ttgaatcatg
    16141 atggatttga ccaaatcgaa ttttacatta cgaccaatcc aggagagcca ctgaagcctt
    16201 tggtgaaagt cgcttctggt ggcgaactgt ctcggatgtt gcttgctttg aaaactattt
    16261 tttcttctga gcaaggtgtg acaagtatta tttttgatga agtggataca ggtgtgagcg
    16321 gaagagttgc acaggccatc gcagataaaa tttctcaaat atcgaaatac tctcaagttt
    16381 tgtgcatcac tcatctgcct caagttgctg cagtcgccga ctatcaatat tatattgtga
    16441 aagcagtgat tgatggacgt acgcaaactt ctgtttcaga gttgaaaaca aaagaaagag
    16501 aagaagaaat tgcacgtatg ctggctggaa gtgagattac aaaactcaca ctcgaacatg
    16561 caaaagaact attgaaactc gcaaaaagga cctgagccac ctcgcagtgg ttcaggtcct
    16621 tttcagcagc tgatagctat attttatttt tacatgacac tcattacagc agtcaataca
    16681 acagatccaa tcgttaaaat cagcattgtc caaatgacaa attttgttac tttagcaaaa
    16741 gtacttgttt gttttttttc acgcattgtt ttcaacctcg tcattaatta gttgattcaa
    16801 gtgtacaatc ttttttccat ttccacaaga gggctttttt ataaaaactt cccttttttt
    16861 aaataatagg tagttacgat acaaataaat aatgttaata gaactagcca tataaaggca
    16921 tcatcccttt cagcaatagg taacttaatg ttcattccat agatactgcc gacgattgtc
    16981 ggtatagtta aaataatcgt taaagaagtt aaaattttca taatattatt cagattattc
    17041 gatacgattg ctgaaaaagt ctgattcatt tgttctacta gttttaattg aatgcgagaa
    17101 gtagtcattg cctgctctgt ttccaccaat acatcacgta aatgattttt caaagcatga
    17161 ttttcttgga aaagtttatt ttgcgctagt tctttcaatg catctaaatt agcagaagtc
    17221 gcttctctga aataaacgag gcttttttgg atatccatga tttgataaag ttgtttgttt
    17281 tcagtggata cttgaagttt ggcttccaaa gtatcgcatt gagattgaag cgtagctaaa
    17341 taactgttgt aagagagtgt gacctgccaa atgaattgaa agaagatatt gagattaggc
    17401 gaaatatcac tttttggaaa ggtctggcta aagactttct ttaaaaacag tggttcatga
    17461 ttcgtaattg tgataagctt ctttttgggc gtcaaaataa tagagagcgg gtatgtatcc
    17521 acttgaaaat aaccgctagg acttgtttta gcaaaaggat attgtaacaa taataacgct
    17581 ggctttgtca actttgattg attgaattct tctgtacggg aattttctgc atcgtccaat
    17641 atggaagtga tgtaatctga cggaatttca aattgatgtg tcagctgatg aatctcttct
    17701 tgattaggac gttctacaca ataccagatc gtttggtcag tttcctttcc ggtattacgc
    17761 agtttctcat tatctaatgt aaaatatttg atcatgattt tcctcctgtt gcctgtttct
    17821 tttattataa cggataggga attggaatac atggaatgtg tttgtaagat atagaatata
    17881 actgcataaa tttaaataaa atgaaacagt tgtgtaaagt atatacagta tacttgaagc
    17941 aacgataaaa aaggagttta cgttcatggg cacattcaca ccacttatct tgttttggta
    18001 tatttacccg gtaatcgttt tgtttggttg ccaatttctt gtaaaattat tttcgttaaa
    18061 tcgtcgtttt aaaataaaag cccctgattt ggcgattcct tttttgtggg gcggtatgca
    18121 tgcattgtct agaaatacag ggacaatttc tatcttaccg tttttcttta tttctgtttt
    18181 gctaatggga atttgtattg ctgttttcca agcatattat tatgaagaaa ttatttatcc
    18241 gcgttatttc aagatgactt ggcgtttggt ttttctgttg acaatcgtct tgtacgctgt
    18301 cttgatcatt gtgaacatac tttcttacct atgaaaaaaa tagaaaagct gtaaaaagtc
    18361 ttttaaaagg ctttttgcag cttgattttt aaaaaaatgt ttatgaataa aaaaagttgt
    18421 tcccggattt cccagaaaag gtggtagaaa gtgggggatt gtggtagact aattttagtt
    18481 agtggaggaa tggggggctt tcaatgttga tgggtgaatt ccaacataat attgatgcaa
    18541 aaggacgttt aatcgtgccc tcaaaacttc gtgaagaact tggcgaaaag tttgttctga
    18601 cacgcggatt ggatggctgt cttttcggct atccgatgag cgaatgggaa aatctggaag
    18661 ctaaactgaa cgaaatgccg ttggctaaaa aagacgcacg tacttttgtg cgattcttct
    18721 attcggctgc cactgaatgc gaacttgata aacaaggacg aatcaatata cccagcacgt
    18781 taagaaatta tgcagctttg acaaaaggtt gcgtagtcat cggtgtatct aaccgaattg
    18841 aaatttggga tgagacacga tggcaagatt tttctgcagc agcagaagag aactttgacg
    18901 aaattgctga aagtatgatt gatttcggac tataagaata ggaagaggct gactaatgac
    18961 tgaaacattt caacactata cagtcatgtt gaaagaaacg gtagatggat tgaacatcaa
    19021 accggatggc gtttatgtag actgtacact cggcggtgcc ggacatagtg aatatttatt
    19081 aagccaacta agttctaaag ggcatctgta tgcgtttgac caagatcaaa aagcaatcga
    19141 tcacgccaag ctgcgtttag cttcttatgt cgaacaaggt atggtcacat ttatcaaagc
    19201 gaacttcaga gaattagaag aacgcttgag tgagcatgta gataaggtag acggcatttt
    19261 gtatgatcta ggggtttcct ctcctcagct agatgaagca gaacgtggat ttagttatca
    19321 tcaagatgca ccgttggata tgcgcatgga tcagtcagct cctttatccg cttatcacgt
    19381 aattaatgaa tattcctacc atgaattagt aaagattttc ttccgctatg gcgaggagaa
    19441 attttccaaa caaattgctc gtgaaatcga acgagtacga aaaacaaaac caatccaaac
    19501 aactggtgag cttgtcgaaa tcattaaaca agtcatccca gctcccgcaa gaagaaaagg
    19561 cgggcatcct gcaaaaagaa tttttcaagc tgtgcggatt gcagtaaatg acgaactcgg
    19621 tgccgaagag gcctctttag aacaggcaat ccggttattg aaagtcaatg gaagaatcag
    19681 cgtaatcaca ttccattcat tggaagacag aattgtcaaa agtatgttta aagaatatag
    19741 tacggtacaa gatctaccac cgggacttcc tgtcatacct gacgaattcc aaccagaact
    19801 gaaagtcatt actcgtaaac caatccttcc ttcagaggaa gagctaacag aaaacaatcg
    19861 atcacgcagt gcaaagttac gtattgcaga aaaaataaga atgaaagagg agtagaatga
    19921 tggcagaatt aaagaaaatg caagattacg aatatgattt acctgaattg gtggatcagc
    19981 cggaacaaca aatgcctgat tcttcttcga atcaagaaat cattcttcca caatcaccgg
    20041 caaaacgatt aaagcagatt tctgctttgg aaaaaattgc tgtggcaagc gtggcgattg
    20101 ctattgttgc tttatgtgtt ttaacagtta tgctgcgcac aaatatcagc ggagctgaaa
    20161 aagaaatcac gtcaatacaa acagaaatca atgataaaaa gcaagaaaaa acgagtttag
    20221 aacaagaaaa aaacgaactt tctcgtactg aacggattaa acaaatcgca gaagaaaaag
    20281 gattgtctat taatgacgac aatttaagga aagtgaagta aatgagtctt aaaaataaaa
    20341 ttcggcggtt tatggaaaag aaaaatctaa atccgatgaa caatcgcaaa aaagtaggta
    20401 tcattttatt tgctacgagt attggattgt tctttttatt tgccgtccgt ttctcgtata
    20461 tcgttattgg aggtcatgtt gctggtacct cattagctga aaaaacaaaa cagttgtacc
    20521 aaggaagtga agtagtaaaa gcaaaaagag gaacgattta tgatcgtaac ggtgtggcgc
    20581 tcgcagaaga tgcctcctct tattcaatta aagcgatact ctcaaaaaca tatacctctg
    20641 gggataaaaa actgtatgtt gaagaaaaga attttgataa aatagcagaa atcctgcata
    20701 agaatctttc tatagacaaa aaagatgcac tgaatatttt agaggacggc gcaaaaaaag
    20761 agctgtatca agttgaattt ggtagctatg ggaaaaatat ctcccaagaa accaagcaaa
    20821 acatcgaagc ggacatgaaa aaagaaggtg tcgctggtct ttattttgtc gaccatcagg
    20881 ctcggatgta tccaaatggc gtcttttctt ctcattttat tggatacgca gttccagaca
    20941 aagatgagaa tggcttagtt ggtaaactag gtttggagtc tgcttacaat gatatcttga
    21001 gcggtaaaga tggtaagatt atttatcaaa aagataattt tcaaaatcct ctgccaggta
    21061 cagtggcaga agaagaaaaa gctgtcgacg gacaagatat ctacacgact ttagacagca
    21121 gactacaaag ttatttagaa acactgatgg atcaagtaaa tgaagaatat cagccagaag
    21181 aattgacagc agtcctgatg aaagcaaaaa ccggagaaat cttggcaatg ggacaacgac
    21241 ctactttcaa tcctgaaaca atggaaggat tgactggaaa agatgctatc tggcgtaatt
    21301 tcttggtaca agatagttat gaaccaggat caaccatgaa agttttcact actgcagctg
    21361 ctatcgaaga aggagaattc aacgagaatg aaaccttcca atctggaaaa atccaagtag
    21421 aagatgctac gatcaatgac catgactttg gtgaaaaagg tgtgctgacc atgcgtcaag
    21481 ctctttcatg gtcaagtaac gtcgggatgg tcatactgga acagcgatta ggcggacgat
    21541 ggtataacta tttacaaaaa ctgggatttg gacaaagtac tcattctgga ttagatgatg
    21601 aagtaaacgg tgctttacct acattaaata ttgtcgaccg agcaatgagt gcttatggac
    21661 aagcggtagg tgtgacgaat ttccaaatga tgaaagcttt cacttctatt gcgaataacg
    21721 gtacaatgat tcaacctcga tatatcagca aagtggtgga tccccaaact ggagaagagc
    21781 gaacaactca aacagaagtt ctagggcaac ctttttcaaa agaaacaaca gaaaaagtcc
    21841 gtgaatatat gagagatgtt gtcgaaagcg aaaactacgg tagtgcttat ggtgtatata
    21901 gtgtacctgg atacaatgta tcggcaaaaa acggaacagc ccaaatcgct tctgatacag
    21961 ggggatatca aacaggagat acagcctacc tgtattcgat tgtggagatg gttccttctg
    22021 aagatccaga ctatgtcttg tatctaacaa tgaaacaccc taagacatat gatcgaatgg
    22081 cattggctaa aatcgcaaat ccattgatga aacgggcaat ggattttaaa gaaactgaag
    22141 aagattcaga tacagaaaca aaaacagaaa aagtatccgt agccgattat cgtaatcttg
    22201 aagcggacgt tgcagcagct gatgctcaaa aaagtggact gcaacctgta gtgatcggta
    22261 atggtaaaaa agtacaaaaa caatctactg ccaacggtga tcagttgatc tctggagaaa
    22321 aactgatcct atatactggt ggtgataagc tgatgcctga cgtaacagga tggtcaaaag
    22381 cagatatcat gaagctgggg aaaatcctcg gtattgaagt tagttttgat ggagatggtt
    22441 attgtgtcaa acaagaactt gctccatatg aaaaaatcac tgaagacaaa cttagtttta
    22501 ctttagaaga ataagaagag aaataggaga gaaaagagca tggaatggac acaagcattg
    22561 attcccattg tcagcagttg tgcaatgaca attgccgcaa tgccgctatt tattggttat
    22621 tttcaaatga aaaagcaagg tcaggcgatt cgtgaggagg gaccaaagtg gcacaatagc
    22681 aaagccggaa ctcctacaat gggcggctta gtgtttctga tcggctctat tttgacagga
    22741 atttgggttg gagcttggca aaaacagcta actcctactc ttttcatctt gctttttgta
    22801 ttagcgttat acggagtaat cggctttctt gatgatttta taaaaatatt caagaaaaga
    22861 aacatgggac tgaactcgaa gcagaaattg attggacaga ttctaggggg aatcatcttt
    22921 tatcttgttt accgttctga aggttatcca ggtgtattga atttcttcgg actagatcta
    22981 cctttaggaa tcatttatgg tattttcgct ctcttctggc ttgttggatt ttcaaatgca
    23041 gtaaatctga cagatggcat cgatggatta gttgctggct taggaagtat ctcttttgca
    23101 gcttacggtc taattgcttg gcatcagcat cagtatgatg tattaatcat ttgcttaagt
    23161 gttttaggag gtttactggg tttcttcgtc tacaacagaa aacctgccaa gatttttatg
    23221 ggagatgtcg gttcattagc gttgggcgga cttttagcgg caatttctat aatgctaaac
    23281 caagaatgga cattgctttt gattggtttg atctatgtta tggaaacagc aagtgtgatg
    23341 cttcaagtta cttcctttaa gcttacagga aaacgaattt ttaaaatgtc tccaattcat
    23401 catcactttg aaatgtgcgg atggtcggag tggaagattg atacagtttt ttggttgatc
    23461 agcattgtga cttcgttgat cacattatgg atcgtttggt aggagatgac gggaaatgaa
    23521 aaaaatatca acatatgaaa ataagaaagt gcttgtctta ggactcgcaa aaagcggagt
    23581 aagtgcggcc aaattattac atgaattggg cgcacttgtc accgttaatg atggaaaacc
    23641 ttttgacgaa aatccggaag cacaagagct gctttcttta gggataaaag taataacagg
    23701 tagtcatcca atcgaattgt tggatgaaga attttcattg atggtcaaaa atccagggat
    23761 tccctacagt catcctttag tagcaaaagc ccaagaaatg ggtattccgg taattacgga
    23821 agttgagcta gcttacgaag tagcggaatg cccaattatt gggattacag gaacgaacgg
    23881 aaaaacaacg acaacaacga tgacaggact actactgaat gcaggagcag atcaaggaat
    23941 tgcacgttta gcgggtaata ttgggtatcc agcaagtggt gtagcgcaag aagcaaaatc
    24001 agaagataaa attgtgatgg aattgtctag cttccaactc atgggaatca cagatttccg
    24061 ccctcatata gcggtgatta ccaatatcta tgaagcacat attgattatc atggtacaag
    24121 aaaagaatat gtcaaagcta aatggaacct tcaaaaaaac atgacagaaa aagattatct
    24181 gatccttaac tggaatcagt cagaactaca agaattagcg caaagaacaa aagcacgcgt
    24241 actgcctttc tcgacaaaag aagtgttgga agacggtgtg tatgctgacg attactcaat
    24301 atactataaa aaagaaaaaa taatggaaat atctgaactt ggcgtaccag gtaagcataa
    24361 tgtagaaaat gcactagctg ctatttctgt agcaaaattg tatggtatat caaatgaagc
    24421 tatacgagaa acgttgcatt ttttccatgg cgtaccacat cgtacgcaat atgtaggaga
    24481 aatccaaggc agaaaatttt ataatgattc aaaagcaacc aatattttag cgactaaaat
    24541 ggctctaagt ggatttgaaa cgtctaaggt cgtgttattg gctggcggat tagacagagg
    24601 aaatacattt gatgaattaa ttccttcatt aaaaggaatc aaagcaatgg tcgtatttgg
    24661 ccaaacaaaa gaaaagttga tggatgctgg aaaaaaagca ggcatcgaga caatagtaac
    24721 agccgattct gtagaacaag cagttccttt agcattagaa aacagtacag atggtgatgt
    24781 agttttatta tcaccagcga atgccagctg ggatcaatac ccaaattttg aaactagagg
    24841 aaacagattc atggaagctg tgaaccgatt aaaatagaaa aagtagaagc gagcgataaa
    24901 aaatgaaaat tttggttaca ggcggaggaa cagggggaca tatttatcca gctcttgcct
    24961 ttgtcaatta tgtaaagact caagaaccga atgctgaatt catgtatgtg ggagcaaaac
    25021 gaggtctaga aaacaaaatc gtccctgata caggaatgcc tttccatacc ttggagattc
    25081 aaggatttaa acgaaaaatt tccatgcata acatcaagac ggtccgattg tttcttaaga
    25141 gtatccgtca agcaaaaaaa atcctcagtg attttcaacc agatatcgtt atcggtactg
    25201 gaggatatgt ttccggagcg gtagtgtacg ctgcagccaa aatgggtatt ccaacagtta
    25261 tccatgaaca aaacagtgta ccaggtgtta ccaataaatt tttaagccga tatgtggatc
    25321 ggatcgctct ttcatttaaa gatgcggctt ccttttttcc tgaaaacaaa gcagttcttg
    25381 taggtaatcc tcgagcacaa gaagtagcag atactaaaaa atcagatatt ttgaaaacat
    25441 ttgatttgga tccagaaaag aaaacagtct tgatttttgg cggtagtcaa ggagcactta
    25501 aaatcaatca agcagtatca gtttttttaa gtgaatatat tccttcttct taccaagttt
    25561 tgtatgcttc aggagataga tattatgaag agatcctaaa taaaataaat cctgaaatac
    25621 aaaatattag tgtccagcct tatatcaaaa atatggcaga ggtcatggct tccagtgatc
    25681 tgctggtggg gcgtgctggt gcaacttcga tcgcagaatt cacagctctg gggttaccgg
    25741 cagtgttggt tcccagccca tatgttacga atgatcatca gactaaaaat gcaatgagtc
    25801 ttgttcacgc aggagcagca aaaatgattg cagataatga actaacaggt gaatcactaa
    25861 gtcagacagt caacgaaatc atgggtgatg aagaattgca aaagcaaatg tgccgagctt
    25921 ctaaagaaca aggaatccct gatgcttcaa aacgtcttta tgacttagta aaacaaataa
    25981 tcaaaaaata aaggcggtgg tcttagtgag taagaaaaaa gaaccaaaaa aattgtctca
    26041 tccgtcttct gatgaaaaca atttgactcc ttggcaaaag gctaaccgaa aatatctggc
    26101 agaacaagag atgaaaaacc aagagccaaa aaaagaagac ataacattag atacacccct
    26161 ttcagattct gaagaagaaa aaacggttca agaggaacag caagaagaaa gccagtccga
    26221 gaaaaaaatc gaattttttg aagagatcaa gcaaaagaaa aacggtggac catataacgg
    26281 ttcttttttg aaccggctgc ccaatttaaa aagtcaacgg cacaaagtac tatttcgtcg
    26341 gttagctttg atcatcactg tattgggtat accgctgatt tttttgatct attatgtttc
    26401 tccttatagc aaattacaag cagttgcagt atcaggcaat aagacagtca acagccaaga
    26461 aatcatctct gatacaaaat tgtcattagg agaaaatgtt tggggacagt attttcatcg
    26521 atctacttat atcgatcgcc ttaaaaaagc acagccaaga atcgaaacag caaatataca
    26581 tttcaaaggg atgaatgaat tcgatttgga tgtaacagaa tataaagaga ttgctttgat
    26641 cgcaaaaaac aatcaatact acccagtgat tgaaaatgga acagtattag atgaaaaagt
    26701 agcaaatcct acaaaaaatc tccctatctt ggaagagttc aaagactctg caaaaatcaa
    26761 agaattaaca aagcaataca atcagttatc atcagaatta caaaaagcaa tttctgaaat
    26821 caagtacacg ccaagagcaa gcaataaaaa tctgatccaa ttgaatatga atgacggcaa
    26881 tcaagttatt gtaaatatca ataatcttgc gaaccaaatg aaatattatt cgcaagtagc
    26941 caaagatatg gatgaaaaag gtgtgatcga catggaagtt ggtatcttct cgcatcctta
    27001 cggtagcact caagaaacgg aagaatcggg ccaatcggaa gaaacaactg aaagtgaaga
    27061 aaatgcggat caaacaaacg aatcaagtga agaggcagaa acgcaaaaag cacaaacacc
    27121 tggatcgtct agcacaaacg gatcatctga ttcaaatgat tcaagtgaaa caagtcaaat
    27181 agaagataat ccaagtacaa ctccgtcaag tttaaatgaa aattgataaa acaactttaa
    27241 aaagtaagaa tgtactttct aaaaaccagt aatttatggt agaattggaa gcagtgaata
    27301 tatctattaa taagtatatt ttaaaacgga cgctagttgt tcgcttgtat atggaatagt
    27361 aagtaggagg gaatcccatt catggcaaaa acaggaatgt atgtaggcct tgatattgga
    27421 acgacatctg ttaaagttgt cgtggctgaa tatatcgaca gccaaatgaa tattattgga
    27481 gtaggaaacg caaaatcaga agggattaac cgaggcatta tcgttgatat cgataaaacg
    27541 gtccaagcga tacaacgagc agtacgacaa gcagaagaaa aagcaggtat ccagatcaaa
    27601 ggcgtaagtg tcggtttgcc agcaaacatg cttgaagtag aaaattgcca gggaatgatt
    27661 gcagttaacg gagattcaaa ggaaatcacc gatgaagatg tcagaaatgt ggcttcagct
    27721 gcccttgttc gttcaatacc tcctgaacgt caaatcgtgt caatattgcc acaagatttt
    27781 actgtcgatg ggttcgaggg aatcaaagat cctcgtggaa tgatcggcgt aagattagaa
    27841 atgtacggtc tgttgtttac aggacctaaa acgatcatcc ataacatacg taaatgcgtg
    27901 gaaaatgccg gattaatcgt caacgaaatg gtgattactc ctttagcgct gactgaatca
    27961 attctttctg acggagaaaa agattttggt acgatcgtca ttgatatggg tggcggccaa
    28021 acaacaacgg ctgttatgca tgataagcaa ttgaagttta ccaatcttga ccaagaaggt
    28081 ggcgaatttg tcactaaaga tatctctatt gtattaaata cgtcatttaa caatgcagaa
    28141 gcgttgaaaa taaactacgg ggatgcatac ccagaacgga catcaccaga tgaagaattt
    28201 ccagtagatg tgattggtca gtcagaacca gtgaaagtcg atgaacgcta cttgtcagaa
    28261 attatttcag ctcgaatgga acagattttc aacaaagcaa aagatgcatt ggaccaaatc
    28321 gaagcattag agttgccagg tggcgtcgta ttgacaggcg gagctgctag tcttcctggt
    28381 gttgtggact tggcacaaga aatcttcggc gtaaatgtaa aattgtatgt tccaaatcac
    28441 atggggctga gaaacccggt atttacaaat gtaataagta tcgtggatta ttcagcaaac
    28501 ttgagtgaag tgtatcaatt ggcaaaaatt gctgttacgg gtgaaacctc agcagctcgt
    28561 caaatggtag ttgaacaaga aacaacaaac acatatgaga gctatgaagc acctgaagaa
    28621 acaatctacg atgagccgga accaaaagag tccggagaaa atgtaaaaaa taaaatcaag
    28681 ggtttcttta caaatatttt tgattaattc atcctgggag gaataagata tggaattctc
    28741 aattgataat aatatcaatg acggcgcagt aatcaaagtc atcggagtcg gtggtggcgg
    28801 tggaaatgct gtaaaccgaa tgattgaaga aaacgttaaa ggtgttgaat tcattacagc
    28861 caacacagac gtgcaagcat taaaaaattc aaaagctgaa acagttatcc aattaggccc
    28921 caaatatact cgtggtttag gtgctggttc tcagccagaa gttggacaaa aagcggcaga
    28981 agaaagtgaa caatccttac gcgaagcttt agacggtgca gatatgattt tcatcactgc
    29041 aggaatgggt ggaggaaccg gtacaggtgc tgcaccaatc gttgcaggta tcgcgaaaga
    29101 attgggcgca ttgactgttg gtgtcgtgac acgtccattc acttttgaag gaccgaaacg
    29161 tggccgtttt gctgcagaag gtatcgctcg tttgaaagaa aatgtggata cattattaat
    29221 tatctcaaat aaccgtttat tagaagttgt agataaaaag acaccgatgc ttgaagcttt
    29281 ccgtgaagca gataatgttt tacgacaagg tgtacaaggt atctctgatt tgatcacagc
    29341 gccaggttac gttaaccttg actttgctga cgtgaaaaca gtgatggaaa accaaggtac
    29401 agcattgatg gggatcggtg tggctagtgg agaagatcgc gtagtagaag caacgaaaaa
    29461 agcaatctcc tctccattgc ttgaaacatc tattgatgga gctgaacaag tacttcttaa
    29521 catcacaggc ggtttggata tgacattgtt cgaagcacaa gatgcttcag atatcgtagc
    29581 aaatgcagca actggcgatg taaatatcat tctaggtaca tctatcaatg aagaaatggg
    29641 cgatgaaatc cgtgtgacgg tcatcgcaac aggtatcgat gaatcaaaaa aggaacgcaa
    29701 gtctagccga ccagctagac aagcacaaat gcaatcacct gctcaaaaga ctgtattaga
    29761 tatggatcaa gctaaaccaa cttcttcaga agaagaaaac agttttggcg attgggatat
    29821 cagacgtgaa caaaatgttc gtcctagagt tgatgattcc aattttgaca atatcgaaaa
    29881 aaaagaattt gatacattca accgcgaaga aacaaaatct catggcgatg atgaattaaa
    29941 tacgccaccg tttttccgtc gtaaaaaata aaggagagga agagcacatg attgctgaca
    30001 acttgcagaa aatcaagcaa gaaattcagg attcgtgtgc tcttgttcat cgtgatccga
    30061 aagaagttac attagtagct gtaacaaaat ctgttgaatc cgctcaagct gaagaattgg
    30121 ttagagctgg cgttatgaat tgtgcagaaa accgagtaga taaattatta gaaaagaaac
    30181 aagatttatt tgattttcct gaaataaagt ggcatttaat tggtaattta cagcgcagaa
    30241 aggtaaaatt gattgtaaat gaaattgatt actttcatgc gttggacagt ttaagattag
    30301 cagaagaaat ccagaagcgg ttagagaaag aattatcctg ttttattgaa gtaaatgttt
    30361 ctggtgagga atcaaaacat ggaatttcac cagatgagct tttaccgttt gtggaatctt
    30421 tagctgcctg cacaaaaatc aaggttgttg ggttgatgac tatggcgcct aaagaagctt
    30481 ctgatgagca aatccataaa gtgtttggta cgctgaaaga attgcgtgat cttgttcaag
    30541 caaaaggtta tgcccatgca ccatgtagtg aattaagtat ggggatgagt caagactttc
    30601 aaattgcgat tgaagaaggt gcaacattta tccgtgtagg aacagcgcta ttcaaagacg
    30661 aataaaggag gaacaaaaat gccgctttta aacagaggag caattgctaa ttttttcgga
    30721 ttagctgatg aggaagagta cgaataccaa gattatcctg aaacaacaaa aaaggctgta
    30781 cagccaacta aaaaaaatac aacacagaat caatcaactc attctgtatc tgctgcgaat
    30841 actcatgaaa aacaaaaaac ggcgacttca cgtcctgtaa cgcatgaaag tattcagccg
    30901 gctgaaaaga aaaaagtaat tgaactacat caatcaccta caaaaactgt agcgaaagaa
    30961 agaacagctt caagaacctc tacacaacct aaagcaaatc ttgctggaaa aatcactgta
    31021 atggaaccaa gagcttattc tgaagctatg gcgattgcta aacgaatcat tgctggagaa
    31081 gcaaccttag tgaattttca tttagtagaa gaaaaacaag cgagaagaat cgttgatttt
    31141 ctaacaggaa cagtttatgc actagatgga gatatcaaac gtgttggaga tgagattttt
    31201 ctttgtacac cgactgggat ggagattgac agttcaaccg ctcaatcttt tgcagatgcg
    31261 aatttgtttg atctgtaaga ggaggaacta cgatttatac tattgcacta ttacttcatt
    31321 tacttcaacg ggcagcctat ttctatagca ttatcttgat ggtatatgca ttgctctctt
    31381 ggtttccagg cgggtatcaa tcagggctag gacgattatt agctaagatc tgcgaaccgt
    31441 atatcagttt gttcgatcgt ctgcctttga ggattggacc cttggatttc acaattttag
    31501 gggcaatcct gatcttgaac ttagccgtac aaggtttagc acagatcgtt atatggataa
    31561 ctgtccattt agtttattga ttgaagcaac aaaatcttta ttctcagaat agataggagg
    31621 ggaaaatttt ggatgcaaat gtataccagc actttcgggc agatgaacgt acatttatcg
    31681 attcggtagg cgattggatt gaacaggttc aaggacaata tgcaccctat ctgaccgaat
    31741 ttcttgaccc aagacaatcc tatattttag aaacactgat tcgacaaagc agtgatttgc
    31801 gtttcatgtt ttatggcgga tatgaacagg cagaaagaaa aagatgtttg attttccccg
    31861 attattacga acctgtagag gaagattttg aaacagcatt gtatgaaatc cattatccta
    31921 gcaaatttgc cagcctaagt catgggaaaa tccttggaac tttagtggga acggggatca
    31981 aacgctctta ctttggcgat atcatctcgg acggtgatcg ctggcaagta tttattgcaa
    32041 atgaggtgga tcattttgtt gctgcacaag taacaaagat tggaaaggtt gctgtacgct
    32101 tggaaccaat caaatacaca gaaatgatta tgccgaaaga tagctggaca caagaaagag
    32161 gaacagttag ttccatgcga ctagacagtg tgatctctga gatttataat atttccagac
    32221 agcggtccaa gcagctgatc gaagctggta aagtaaaagt gaactggaca gaaaatacaa
    32281 gacctgattt tgtacttgaa ctattagata ttatttcagt cagaggttat ggcagagtcc
    32341 agatccaaga acttgagggg aagacgaaaa aagaaaagta cagggtactg ttcggtgtat
    32401 tacgtaaata aaataaaaag gtggtttact catggcttta actccattag atatccaaaa
    32461 taagacattc ccgacaaaaa tgcggggata taatcaagac gaagtagatg atttcttaga
    32521 tttagttgtt cgtgactatg aggaattgac tcaaagaaat cgagaattag aaaaagcagt
    32581 caaacattct gaagaaaaac ttgagtactt caatgaatta aaagatgctt tgaaccaatc
    32641 aatcatcgta gctcaagata cggctgataa agtaaaaaca agtgcaagca aggaatccga
    32701 agtgatcgtt acttctgcac aaaacaaagc agatgagtta gttgcgaacg ctgaaaaacg
    32761 tgcacatcaa ttaacaactg atgcagaaga aaaagcaaga aaaatcttga cagatgcaac
    32821 agaaaaagca agacagcttg cgacagaaac tgaagatctt aagaaaaaaa cacgagtatt
    32881 ccatcaacgt atcagcttga tgcttgagtc tcaattagaa caagtgaaaa gtccggaatg
    32941 ggatgaaatt ttacaaccat tctccagcta cgtgactgac agccatgaag tgatcaaaga
    33001 agttttagca aaacagcttg acaatgaaaa tgatactgat gtaaactcag acacaggagt
    33061 catagaagca cctatgacgg catttgatac acaggctatt gatatctcga tcgtgcctgg
    33121 tgaaaatgaa aacgagtaga acagaaaagc actttgttat tcttaaagcg agccgatgag
    33181 agtggaagtt cggtaagaca ctgcatggtg aaagatcctc tacttgagac ctgcttttcc
    33241 gaaattagag taagaagcag cggctgacct cgttattggt cacagagagt ttgcactaga
    33301 tagatgcaac taaatttggg tggtaccacg gcatgttcgt cccttgcggc tgaacatgct
    33361 tttttgtgtc cggaaaacaa ataaaggagt gtatggttga atgaaaatga aagaaacctt
    33421 gcaattagga aaaacaaaat tccctatgcg tggtaatttg ccaaaccgag aaggcgagtg
    33481 gcaaaaagaa tgggaagaaa atcgaatcta ccaaaaaaga caagagttga acgaaggaaa
    33541 acctagcttt gttcttcatg atggacctcc atatgccaat ggtaatatcc atttaggaca
    33601 ttcattgaat aaaatcagta aagacattat cattcgttca aaatcaatgt ctggctatcg
    33661 tgcaccatat gttccaggat gggatacaca tggattgccg attgaacaag tattggctaa
    33721 taaaggcgtg aaacgtaaag aaatgtccat ggcagaatac ttggaaaaat gtcgtgaata
    33781 tgcactttcc caagtagata agcaacgagc agatttcaaa cgcctaggag ttgcaggaga
    33841 ttgggaacat ccttatgtta cattagatcc ttcttatgaa gctgcacaag tacgcgtatt
    33901 tggtaaaatg gctgaaaaag gctatattta caaaggattg aaaccaattt actggtctcc
    33961 gtcaagtgag tcttctttag cggaagctga aattgaatat aaagatgtaa aatctccatc
    34021 tatttttgta gctttcaatg tcaaagacgg caaagatatt cttgatgaag atacagcttt
    34081 tgtcatttgg acgacaacac catggacatt gccagcaaac caaggtattg ctgtaaatcc
    34141 aaaatttacg tatgtattgg tggaagccga tggccgtaag tttgtcgtag cgaaagattt
    34201 gctagaaaca gtccaatcag aaatcggttg ggaaaatgta acagttctaa aagaatttgc
    34261 tggacaagaa atggaatata tgactgcaag tcatccattc tatgaacgtg aatcacttgt
    34321 gattttaggc gatcatgtta ctttagacgc tggtactgga ttagtccata ctgctccagg
    34381 acatggggaa gatgactata tcgctggtag caaatataaa ctatctgtag tgtcacctgt
    34441 agactcaaaa ggattgttta cagaagaagc acctggattt gaaggtatct tttatgacaa
    34501 agcaaatcct atgatcactg atctattaaa agaaaaagga gctttgctga agctagattt
    34561 ctttactcat agttatcctc acgattggcg gacgaaaaag cctgtgatct atcgggcaac
    34621 tccacaatgg tttgcatcta tcgataaatt ccgtcagaat attttggatg aagtagaaaa
    34681 agtagagtgg gttatcccat ggggtaaaac acgtttgtat aatatgatcc gtgaccgcgg
    34741 cgattgggta atttcccgtc agcgtgcatg gggagtacca cttccgattt tctatgctga
    34801 aaatggagaa gcaatcatca caccagaaac aatcgaacat gtggcaaaat tatttgctga
    34861 acatggatca attatctggt ttgaaaaaga agcgaaagaa cttcttcctg aaggatttac
    34921 ccacccaggt tctcctaatg gcgaatttac aaaagaaaaa gatatcatgg atgtctggtt
    34981 tgattctggt tcttctcatg aagctgtttt acgtcagcga ccagaattat cattcccagc
    35041 agatatgtat ttagaaggat cagaccaata tcgtggctgg ttcaactcaa gtatcacgac
    35101 tagtgtggct atcaatggtg tagcaccata caaatcagtt atttctcaag gatttgcttt
    35161 agatggtgaa ggccgtaaga tgagtaaatc tttaggaaac atcattgcac cagaaaaagt
    35221 gatcaaacaa tttggtgcag atatccttcg cttatgggta tctagtgtag attatgaagc
    35281 agatgttcgt gtttctatgg atatcttaag tcaagtggca gaagtatatc gtaaaatcag
    35341 aaatacgatg cgtttcttat tagcgaatac agaagatttc gatcctaaaa aagatactgt
    35401 ttcgtacaat gatttacgtt ctgtagacaa atatatgacc gttcgattga accaagtaat
    35461 caaagaaatc cgagaagaag ggtacgacaa atataacttc atgcatattt accgtacagt
    35521 tatgaacttc ttgacggttg atctatcgtc attctacctg gattttgcta aagatgtagt
    35581 ttatattgaa gcagaagatg attatcaacg tcgttgcatg caaactgttt tctatcaaac
    35641 agctgtagct ttgacaaaat tattgactcc tatcattccg catacagcag aagaaatctg
    35701 gtcattcctg aaagaagaag aggagtacgt tcagttaagc gagtttcctg gttatgaaga
    35761 gtttgcaaac caagaagaat tgatggatac ctggactgct ttcatggact tccgtgataa
    35821 tgtcttaaaa gcattggaag aagcaagaaa cagcaaattg attggtaaat ctttggaagc
    35881 aaaactaact gtttatccaa aacaacaagt tcgtgaaatg ttaaaagctt tggatgcaga
    35941 tattgcacaa ttattgatcg tttcaccaga ctactttgaa gttatggcag cagatgaaca
    36001 agtgccagat aacgctatgg tatttgatga tgttgctatt acagtggaaa aagcagacgg
    36061 ggaaacttgc gatcgctgtc gccaagttcg taaagatgta ggagtagatg aaaaacttcc
    36121 tcatctatgc ggtcgttgtg caaaaatcgt tgaagacttc tatcctgaag ctgtagctga
    36181 aggattcgaa gaaaagtaaa agggaaaagg gggtgtgaca tgttctttgt cacaccccct
    36241 tttgttatca atcgtttaat tttccacgtt cacgatacca gttatctggt tgccagatcc
    36301 attcgaatcc gtctttttca agtagttcga aagctgcttt aggtcccatt gatccagctt
    36361 tgtagttggg aaaatccggt tgtgtttggt cccaagcttt tcgaatgaca tccacgatat
    36421 gccaagattg agcgacttct tcccaatgag agaagttcgt tccatcacca ttcaacgcat
    36481 ctaatagtaa tttttcgtaa gcttctgggc tattttcaac catttctgca ctatttctaa
    36541 attccaattt gattggttcc gtttcaaatc cttgccctac ttctttccca ttcaaggata
    36601 aagaaaatcc ttcagttggc tgaatataga tcgttagaac attaggtgga agtgtcgtat
    36661 cgtcacatgg ttcatcgaca gatgttttga atacattaac aggaacttgt ttgaatacga
    36721 tgttgatccg agtccctttt tcagtcaaac gtttgcctgt acgtacatag aaaggaacac
    36781 ctgaccaacg gaagttatcg atagtgaatt tgcctgcgac gaatgtttca gtctctgatt
    36841 gagcatccac tttgtcttct tcgcgatagc ccttgaattg tacaccatcc aattcacctt
    36901 gagcatattg cccgcgaacg aagttttcta acacttcttc ttcgttataa atgcggatag
    36961 ctttcaatgc tttgatcttt tctgtacgga tttctttttc ggaaaatgct actggcggtt
    37021 ccattgcaag taatgctaca acctgtaaaa tatgattttg caccatgtct ttaagtgcgc
    37081 cactatgatc gtagtagccc ccgcgatctt ctacaccgag gttttcgcca aaagtaattt
    37141 ggatattgtc gatatagcga ttgttccatt gagcctcaaa aatattattg gcaaaacgga
    37201 tagctgagat gttttggatc atttcttttc ctaaataatg atcaatcctg aaaatatctt
    37261 gttctgggaa tacttctctg atttgctggt tcaattcaaa agcagactgg taatcagaac
    37321 cgaacggttt ttctatgatc aaacgattga agccattttt tgtcataata ttttgtgatt
    37381 tcaaatgaga aacgatcgtt ccaaaaaatt gaggtgccat agccaaataa tagacacggt
    37441 taccgtttaa ttgatagtgt ccatctaatc ggtcagacaa ttcttttaat gtctgataat
    37501 gcgttgaatc atttacatca tgggattgat agtaaaaatg gcttgcaaag gtagttgctt
    37561 cttcatctga aggattcaat gcacggattg tttctttgac aacttcacgg tagtgctcat
    37621 cgctccaggg acggcgagcg gttccgatta cagcaaaatg ctcgcctaaa tttccttttc
    37681 tgtaaaggcg gaacaaagac gggtacaatt tacgctgggc taggtcacct gtaccgccga
    37741 atatcgtaaa caatacattt ttttcattag acatgtggat acactccttt atattttatc
    37801 gacaaaaatc gtgctggccg ccttaaagct gacggggatt ttctttctat cattttggat
    37861 agtgatcggt ccttcataag ctgcgatgtc aacgatctca tattcttcgt tgatattcag
    37921 gtcaatagtg actaagtaat ccaacaattc tttttcatca agcactcgtg cgatacgcac
    37981 ttttgtaccg acaggatagg actccaatgt ttgtcggcgt tcttcatgga caacttcctc
    38041 ttttttagga atcgatcctc catgaggaca atgttcagga tgattcaaat agttttctaa
    38101 atgatctgca agatgttccg aagtcacatg ttccaatact tcagcatcat catggacttc
    38161 attccagcta tagtctaaat gttcaactaa aaaaacttcc cataagcgat gtttgcgaat
    38221 cagactgcta gctttttgca gtccgcttgc agtcaattgt acaccttgat aaggggaatg
    38281 ttctacaagc tcttctttca caagtttaga aatcatttca ctgacagatg cagctgagac
    38341 atctagacta gatacgattt gtttattgtt gactttcgtt gtatctccac ctagttctaa
    38401 aatgattttc aaataatctt ctcgatttgg tgtcatattc gatcatcctt tcatggtaaa
    38461 aaaccatcat tagtaagtgt aacaaaaaaa acgttaattg actatttttc taaataaaaa
    38521 acaaaggatt tttagacgtt tttacaacaa aaaacgaaag atgatagaga agactcattc
    38581 tttcgtttat aaaggatata tatatacatt gggagggatc tatttttcag ataacaactt
    38641 atatgaagtg tctgcagatt cgtcataaag agtttgttcg ggataagaaa agataagata
    38701 aaaaccatac tgatcacgta cgactaaacg atattcatct atttctacca cagatgtgcc
    38761 aagagactga ccgttgatca aaaacgtgta atcagatgta atctgtaatt gttgtttttg
    38821 ctggctatca ctaaagaacc atgtaccttg atactttgag agtccttctg acttttccgc
    38881 ttgttttttc tttttcaagt aaataaaact tgagagaacg gtaatgagtg aaccaagaaa
    38941 atagatcatt ttttttatca ccaaatcact cctttatgag aaaataatcg catagacata
    39001 ttctttcttc tttatttcat tgtaccgaat aaaagctaaa atttctctaa gactttcgac
    39061 tggttttttt actgtttttc tacaaattat gataggatac atagtaggaa gaaaggtgac
    39121 ataatttgaa gtatcagcaa gtggaaatag ctttttttat tgaccgcccg aatcggttta
    39181 tagcacattg tatgaaaaaa gacggagaaa taattattgc gcatgtcaaa aatacaggta
    39241 gaggaaaaga ggtcctgata ccaggcgctg aagtggctat tgtatttgct cctggaccta
    39301 aacggaaaac cgcctatgat ctgattgctg taaaaaagaa gggtgactgg tttaatatcg
    39361 atagtcaatt gcctaatcgg ctggcaattg atggtatttt agacggcacg atccttttac
    39421 caggattaac tggtccattt acggtataca agcgtgaagt aacttatctt cactccaaat
    39481 ttgatattta tttagagacg gtggtcggac aaaaagcatt tgttgaagtc aaaggtatga
    39541 cacttgaaaa taaagcgatt ggtgcttttc ctgatgcgcc cacactcaga ggattgaaac
    39601 atataggaga attaacgtat gccgcacaag atggttatgc cgcttatgtc ttgtttattg
    39661 ctcagttcga acatcttcat ctggcaacga tccatgaaga aatgcaacca gcactagctg
    39721 atatggttcg acatgcgcag caaagcggag ttcaaatcct cgcctataat tgtcaagtat
    39781 cggcagatga ggtaactgtc aagcaggcca ttccatttga tgtagatatc ccttttgaag
    39841 atcctataca atagaaagaa ggtaaaataa tgatcaactt aggtattatc ggaacaaatt
    39901 ggattactca tcaatttgtc caagcagctt taagcacaaa aaaatatgaa ttgacagcgg
    39961 tatattctag acatttggaa aaagcacagg aatttggttc tcattatgaa ggggatatcg
    40021 aattcgcaac agatttgaag acttttttca atattactca tatggacacc atttatattg
    40081 cttctccgaa tagtcttcac tttagtcaag ctaagcaagc aattttggca ggaaaaaatg
    40141 tgatagttga aaagccagct ttttcgacgc caaaagaaat ggatgaaatc atccatttgg
    40201 caaatgaaca caatgtgtat ttttttgaag cagcaagaaa tatccatgaa tcaggtttta
    40261 aaaagattgc cgattactta ccattaaaag acgagataat cggtgcaaat ttcagctata
    40321 tgaaatattc ttcgcgctat gaccaagtat tagatggaga agaaccgaat atcttctcga
    40381 ctcatttctc aggtggtgca ttgatggatt taggtgttta ccttgtatat gcagctgttg
    40441 cttggtttgg aatgccaaaa gaagtccatt atttcccaag aaagatttct actggtgtag
    40501 atggtcttgg atgggggatc ttacgttatg agaattttga tgtatcgatc cagccaggta
    40561 aaatcggtga ttcttattta ccgtcagaga tttattttga ctcaggaacg ctagtgatga
    40621 atggagtcaa tgcaattgaa caagcacaat ttcatgatcg cgcgcataaa gaaatcattg
    40681 atttggcggt aacagctgca gaaaatccaa tggcagatga agcagaggat tttgccaatg
    40741 tcatggctca tccgaaagat ccagcttggg gattgttgta tgaagagtgg atcgagctag
    40801 ctagaaatgt gaaccaagtg atttatgatt taagaaaaaa tggcggcatc aaattcgatg
    40861 cagataatga ggaagatttt taatgaacga atttgtacaa gtatttcccg aaaattggca
    40921 gaaaaaatat gaggataaag ggtttatctc accttctctt attcaacgag cagtttttca
    40981 gccactaagt aataaacaaa gtatagtagc ggtttctcct acaggttcag gtaaaacact
    41041 ggcttatctt tggcctctac tactaaacgt ggaaccaggt gaagcaagtg cgttggtcat
    41101 ttttgcttct tctcaagaac tagctattca agtagcagat gttgcacgtg aatggggaaa
    41161 agacaaagag ttgaaagtcc aatctttggt gggcggagcc aacgtcaaac gtcagattga
    41221 gggtttgaag gaaaaaccag aaatcttgat tggtacacca ggaagaatat tggaattgat
    41281 gaaagcaaaa aaaataaaag cacatcaagt gaaaacaatg gtatttgatg aagcagacca
    41341 gctatttgac gctgggaaca gccaaatcat tgatcaaatt cttcatcaag ctccaacaga
    41401 gtatcagcta gctttttttt cagcgacagc agatcgttcc cttgaaagca ttgaaaaaat
    41461 cactgggaaa acgtttgaga caattgacgt gacagcagag gacgattctc gaaaaggact
    41521 gagacattac tttttacgtg taccgatacg aaagaaagat gactacttga gacgtctcac
    41581 gcatatagaa gattttcatg gtctgatttt ctttaatcaa ctgactgaat taggcgtaat
    41641 ggaagaaaaa ttgctgtata gaggtgtacc ggtaggaagt ctggcctctg atcagaacaa
    41701 gctactccga aaagcagcgc tagaacaatt caaaaagaaa atggtcagag cacttttgac
    41761 cacagatgtg gcggctagag gtttagatat tacaggtctt ccttatgtag tgaatgcaga
    41821 agttccgtta agtgaagaag cttatttgca tcgttcggga agaactggtc gaatgggtaa
    41881 tgaaggaata gtaattacac tcgtacaaga taataatcta agagatttaa aacggctttt
    41941 acgtccatca aatataacac ttgaagagat ttttttatac ggaggtaagc tgcaaactga
    42001 gcagccaacc aaagaagatg cagctcctgc agtaaataaa aagtatgcga aaactttttc
    42061 tggaaaggag cagaacccag aatataacaa agaaaaaata agaaaaaaag ttaaaaataa
    42121 atcaaaaaaa gagagaaata aaggagcaag gcgcaaataa gcgccttttt atgctatgat
    42181 tatgaaaacg ggaacaaaat tggttgccag aggaggggtt atgtgaaaga acgtcaagaa
    42241 gagatcttgc agctattgac agaaaaccaa caaggattga cagcaagtga tgtagctgag
    42301 cgcttaacta tcgatcgaag caatgcaagc cgatatttaa gtgaactata caaggcccat
    42361 catattgtaa aaaccgctgg ccgaccagtc gtttactctt tgccaacgga aaagtcaaaa
    42421 tctgatgaag tgcatgtcga ctcttctacg caagtgactt ttgaaacact cgttggagaa
    42481 aatgattctt taaaagtcag catacagcag gcaaaagctg cgattcttta tccaccgcgg
    42541 gggttgcata cgattatttt tggagaaact ggtacaggga agtcgatgtt tgctgaatgt
    42601 atgtatcatt ttgcaatcga ttcagagatg ctttctgcag atgcgccatt tgtttctttc
    42661 aattgtgcgg attatgctca aaatccgcaa ctgctctttg gacacatctt tgggatcaaa
    42721 aaaggggctt acacaggagc agctcaagat agtcccggtc taatagccaa agcagacggc
    42781 ggtatattgt ttttagatga gatccaccgt ttaccgccgg aagggcagga aatgctgttt
    42841 actttcattg ataaaggtat ttaccgtccg cttggagaaa gtgcacaagt acatgaagca
    42901 tctgttcaga tcattggggc gacgactgaa tcttcagaaa gtttcttatc tacgttcaat
    42961 cgacggattc ctatggcaat caccttgccg aatcttgctt cacgttcact ggatgagcgt
    43021 tatgaaatca tctctctttt tatcaaacaa gaagcgaatc gtttgaatca acgtatcgat
    43081 atcgaaaaag aagcgattct tgccttcatg ctttatgatg cggaaggaaa tatcggtcaa
    43141 gtgaaacgag acttgaaact agtctgtgca aaatcttttt tacattaccg tactcatcat
    43201 gagaaaaagc taatcattcg aaaagaagaa ctttcccttc aagtacaaaa aggattactg
    43261 aaaatcaagg aaataccgga acgtctcgac cgattcatgg acagcaaaag tcagtatttg
    43321 acatttgaac caggatttgc tgatgttgtc tggtcacaag atccagaacg aaacatgcag
    43381 gtctataacg atattgagga aaaagtcact tctctttccg aaaccggtgt ggagaacatt
    43441 gatctagaga ccctcatttc aaaagatgtg gatgcttatt tccagacata tgtcaaagaa
    43501 ttgactaaaa gttcgattcc caaagatctt ttgccagatg aaatctggcg gttaaccaat
    43561 caattgtatg atgtggctga aaaacggtta gaccgtattt ataatgaaaa agctcgtttt
    43621 gcttttgctt tgcatctgca aagtacattg gatcgtgtga gagaaggtca tatgatcgtc
    43681 cacccagatt tgaataacgt acggaaaaat tttaagaggg aattccaggt agcaatcgat
    43741 ctttcaacga tcattgaaga agaagaacaa gttgagattc cttttgatga aatcggcttt
    43801 attagtatgt ttctttctat caatctagga gaaagtgagc ctgttaaaga aaacaaagta
    43861 gatgttctca tattgatgca cggcagacag actgcttcaa gcatgttgga gatggcacaa
    43921 gaactattag gaacaaaagt agggaaagct tttaatatgc cactggttat ggaagttcaa
    43981 acaatgtatg aagaagtgtt acaatatgtc aaaaactatc aagagcattt gacgaatggc
    44041 ctactcttgt taacagatat gggatcatta aacaccttca gtgatttgat ttatgaagag
    44101 actggcattc ggacgaaagc tatcacgatg acaagtacca tgattgtgat agaagcgata
    44161 cggatggcaa gtgttggaag aagcttagaa gatatttatc aaaatatcca gctttccttt
    44221 gaatcgatcg tacgtgatca gttcaaagcg gaaaagtctg aaactttaca gcgtaaaaaa
    44281 gccgtaatcg tgacatgctt tactggtgaa ggagtagctg ccaaattata tcagcgtatt
    44341 atgccagtga ttaatcaaaa taaggtagaa ctgatccaga tgcagttttt agaacgtgaa
    44401 tcattcaaaa aacacatcga tgccttattg gaagaatatg agatcaaagc aattgcggga
    44461 acagttgatg ttgattatca aaatatccca tttttttcag cctatgacgt ttttgatgat
    44521 gaaaaactaa atattttaaa gcgtatagct gatgatgaga tcccgacagc taaaattgtc
    44581 gattcacttg ctggatcatt aagcgatgtc ttttctattg atgaactgat ccgttcattg
    44641 caaaagacag ttcagcagat ccagaccgat ttgtcgatta tcgtggaacc tggagtagat
    44701 actggaatcg ttatccattt agccttcatg ttagacggat tgatcaaaca agaaagccat
    44761 cggcaatttg agaaactgtc tattttctca aaaatttatc gtttggaaat ggatatcgtt
    44821 cgggcaaact tgatggcact agaacgaaaa tatcaggtaa aaattccaga agatgatatc
    44881 gcatttctta cacaaatgtt tcttgaaaat aaaataaaaa aatgaacaca ctaatcatac
    44941 tgtttacaca ctaaacatct gtgtgcgcac tataaaaaag agctaaaaag catgaaaacg
    45001 ctatttcagc tctttttttt attggcatgc ttcttgcgtt atataaaagt gtaaaaaaat
    45061 aaaaggagtg ttaaaaatgg caaaaaaaac aatcatgtta gtttgttcag cagggatgag
    45121 tacgagttta ttggtcacaa aaatgcaaaa agcagcagaa gaaaaaggga tggaagccga
    45181 tatttttgcg gtctctgcct cagatgcaga taataattta gaatctaaga atgtcgatgt
    45241 attattatta ggtcctcaag tacgtttcat gaaagctcaa tttgaacaaa aacttgcgcc
    45301 taaaggtatt ccattagatg tcatcaatat gcaagactat ggcatgatga acggagaaaa
    45361 agttcttgca caagcagaaa atctaatgaa ataaatcaag tatgcagttg ggggtataac
    45421 aacgtggaag atcaacaaaa tctagaagcc atcatggggt taatcatgtt tggcggtaat
    45481 gcaaagagcg atgcaatgga agcgattgcg gctgctaaaa tcggtgattt tgagttagcg
    45541 gataggaaaa tcgctgatgc agaagaatca cttatacaag cgcatcatgc acaaacagac
    45601 atgttgacac aagaagcaca aggaaatcat atgcaagtga cattattgac tgttcacagt
    45661 caagatcact taatgacatc tattgcgttt actgatttag caaaagaaat catcgaactt
    45721 tatcgtcgcg tatacagcga ataaatacaa cttctttttt gaacggaaga agaaaaattt
    45781 ttgaacggat tttttatttt gttataagga gaactctctt tatataaaaa acttatagga
    45841 ggaagtaaaa aatgaacgga ctatcaagtt ggatggagaa atacatcctg ccggttgccg
    45901 caaaaatcgg agcgcaaaaa catttagttg ctttgcgtga tgcttttatc ggcacactgc
    45961 ccgcaacaat ggcgggatca gtcgctgtaa tgatcaatgc aattattcgt gatttaccgc
    46021 aacagtttaa cagtacctat gctgacagtt tagcagcaga caaaggaatc tttgccgtat
    46081 tgaatgtcat catcggaatc aatggttttg tctggaacgg tacactagca atcgccggat
    46141 tgatctttgc cttttcttgg gggtataatc ttgcccgagc ttatggagta aatgatctag
    46201 ctggggggat cgttggttta gctaccttga ttcaaggtat ttcctttggt ccagcattat
    46261 cagcagcatt agatgtgaat gttccttcaa atgtcgcaga tgcaatcaat agttctgaga
    46321 ttggtgcaac gattgcagat ggcacactgt cagttggatt atggggttgg ctaaaactag
    46381 atcatttgaa tggaaacgct tattttacag ttatgatcat gggagctatt tcagtcatta
    46441 ttttttgtaa attgatgctt gccaacatta cgatcaaaat gcctgattca gtaccaccag
    46501 cagtatctaa agcatttgca gccattttac cagcgacgat cgctttatac gtcattgcta
    46561 ttatcaatta tatcgtaggt cgtgtagctg atgggcaact gatcattgat ttggtgcaaa
    46621 aatatattgc agaaccattc ctaggattat ctcaagggtt gggagcagta ttgatcgtca
    46681 cagtatttgt tcaagtattc tggttctttg gtattcatgg accaaatgta ttggcaccag
    46741 tgctagaggg aatctgggga caagcacaac taatcaatat tgatattttc caaaaaggtt
    46801 ataacggaaa aactggtaca ccagccgtgt tagatgcaat cgaagatgga aaagcttata
    46861 tgtgggtccg cggatcgttc gatgcttttg catggtttgg cggatcaggc ggaacgatcg
    46921 tcttactgat agctatcttg attttctcta aacgtgcaga ttatctaaca gtagctaaat
    46981 tatccattgg gcctggtatc ttcaatatca acgaaccagt gatgtttggt ctgccaatcg
    47041 tattgaatgc aatcatgttt atcccattct tgattgctcc agtagtagca acagcaattg
    47101 gatggacagc tacttatctt ggcctagtcg ctccggtatc gcagcaagtc acatgggtag
    47161 taccgccgtt cttattgtca ttcttagcaa caggagcgga ttggcgggca ccgatcgtaa
    47221 cattagtatg tatggtcgtg actttcttga tttgggcacc atttgtcatc tctgcaaaca
    47281 aaatggaccc tgccgcaggc gaacaacaat aagatgaacg tgaggagcgg gacgctttaa
    47341 gtttcgttcc tcataatgaa ttttctagga ggaactgaaa tgattacaat tgccagtgca
    47401 atgcaacaag atgaaatcaa agaattgtta gaaggataca atgaagaagg aatgactttt
    47461 acatttaaag aaaaacaagg aatcaagctg ttctttgaaa caagtgcaga agatccggaa
    47521 gttgcggcta aaaaagcaaa ggaactaatc aaagcacagc cttggggaac agtcctttat
    47581 tttcaatcaa cagctgttta gaaaatggtt taaaaagata ggatggttga gatgataaaa
    47641 aacggtttat ttttatgtag tattggttct ttgatcgtgt tgtatagttt gaatccacat
    47701 tcgcaaaggt atgatttgta tagtatggtc acaggtcttt ttctaacagg attaggaatc
    47761 tatttcttca tgaaggggaa aaaaagagaa gaagcaaagg agaaaaataa ctaatggaaa
    47821 tcacgaaaaa attaaatatt ccagcatcgt ttttttacga taaagttatg gactctgtcc
    47881 ttttcgatgt acgtaaagcg accggcaaat cagtaactag aaaacagctg aaaaatttgg
    47941 agtacgtcaa acaattctcg caaaataacc gggctcgcat aaaaatcgaa gaagtcattg
    48001 aaaaccagtc ttataagttt cgtacatcga caacgaagaa tgaattcgtc gttcattatc
    48061 aaatcaaacc attggacgat tcgacctgtg aggttcatta cacagaaaaa atggagtctt
    48121 atggcatgct tcaaaagttg aatgatatgc ttttaggaac aatcttgatg tattttaaac
    48181 ggagacgttt caaaaaaatg ctagatatga tcgaagcaac gtattaaatc aatgaaacat
    48241 ggtactattt agttattgaa aaatgaagta agctagaagg aatttttcat aggaagatag
    48301 atctttcctg cgaatcctca ctagcttttt tgtaggaagg ggcagcaaaa tgacaaacaa
    48361 agtaagagag ctaggtgtat cttcgattga ggaaatgttt gatttagcca cttatgcatt
    48421 taatggggca gatacacctg aaaggcgaga acgttttcgt acacttgcgg agaattcttg
    48481 gaattacgga ttttttgatg aggaggatcg gctaacaagt caagtcatgg cgaccccttt
    48541 tccagtgaat ttttacggac aagagtacct aatggccggc attggctatg ttgcttctta
    48601 tccagaagca aggggacaag gcgggatcaa tcagatcatg gaaaagatcc ttgaagattg
    48661 ccgtgatcgt aaagtaagct tatcttatct ggcgccattc tcctatccct tctatcggcg
    48721 ctatggctat gaacaatcat ttgataaaat cagttatcaa ctaaacagtc gtgatgtgcc
    48781 gtttatcaaa aaaaaatctg gaaaaataaa gcgtatggat tttgaagatg caaaagctgc
    48841 aatcagaaga atttatgaac agatgccgga aaaccaacga gctggtctga agcgcgaaga
    48901 ctggtggtat acgtataaat tcaagcaaaa aggcaattac cagtttgctt tgcattttga
    48961 tgataatgag gaagcaacgg gatatatcgt ttatcagctt gcaacgtctt cctttattat
    49021 tgcggagtgg ggatttttgg atcatgatgc tttttgcagc cttgttcgct tcgtaagttc
    49081 tcacaatggt gcttttgaaa catttgaata ttcatgcggc tacggcggta atgatcaaaa
    49141 ttatctatta gaaaatccat ttgcttctgt aatgatcaca ccttatatga tggcgaggat
    49201 cgtagatatt tctttgtttt tggaaaatta tccatttaaa aagagacaag ctgcatttgc
    49261 cctacaaata gaagaagatc attatgcgaa atggaatgaa ggaatcgtgg aagtaacaat
    49321 cgaaaatgga aaaaatcatc tctcaaaagt agaacaaaca aaactcccaa tcgttcgagg
    49381 aagtatccaa catatcacgc agctattatt agggtatcgt aaagtggaag agttggtatt
    49441 ccaagaaaga atacaggtga caaaagaata tgaagaaacg ttaagctcaa tcttgccaca
    49501 gcaacagcca attcttaatg actatttttg ataaaaaacg ggtgatgaca atatttttgt
    49561 caccaccctt ttgtttttca tttggcattt gaaggaaaat gttaaagtaa atcctctttt
    49621 ttcgtaaatt tgaatttttt gttttctttt gttttagggt tttctttttt gtggtcttgg
    49681 attgcttctt tcgtaatctt ttcccgattt tttttagatg aatctaagcg ttctgcgtat
    49741 actttttctt ttggcttatc attcattgga aaggacctcc ctacattatt tcattctacc
    49801 tctagtttat catctgttaa ggactagtgg aaacgttttg cctaacttat aagctgattt
    49861 ttttagcaga taagcttgca agtaaaaaac aaacaagcta caattgattt attgaaattg
    49921 ttcctgtagc tcagtaggat agagcaatcg cctcctaagc gataggtcgc tggttcaatt
    49981 ccagtcagga acgtataaat aacccctgta tcctttgcgg tacaggggtt tgttttgtat
    50041 ttgtgccaaa aatgtgccaa actagttatc atttttatat aaactatcga acacagtatt
    50101 gattttttct ttatcctgat cttcaagctc tcgaacaata tgaagataag tagacattgt
    50161 agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta agtatattta ctccttgata
    50221 aagtaaaata gatgcatgtg tatgccttag tccatgcaaa gtaaattgct tttcaatccc
    50281 taattctttt aattttttgc gcaaatattt gctgacg
//
LOCUS       DACEMO010000004        36607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEMO010000004 DACEMO010000000
VERSION     DACEMO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.004"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(65..463)
                     /locus_tag="IW728_000157"
     CDS             complement(65..463)
                     /locus_tag="IW728_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1023544.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(460..1233)
                     /locus_tag="IW728_000158"
     CDS             complement(460..1233)
                     /locus_tag="IW728_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1023545.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1230..1913)
                     /locus_tag="IW728_000159"
     CDS             complement(1230..1913)
                     /locus_tag="IW728_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1023546.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2080..2967)
                     /locus_tag="IW728_000160"
     CDS             complement(2080..2967)
                     /locus_tag="IW728_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1023547.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(2964..3833)
                     /locus_tag="IW728_000161"
     CDS             complement(2964..3833)
                     /locus_tag="IW728_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1023548.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(3970..5256)
                     /locus_tag="IW728_000162"
     CDS             complement(3970..5256)
                     /locus_tag="IW728_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1023549.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5529..6107
                     /locus_tag="IW728_000163"
     CDS             5529..6107
                     /locus_tag="IW728_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1023550.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6107..7036
                     /locus_tag="IW728_000164"
     CDS             6107..7036
                     /locus_tag="IW728_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1023551.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7033..8769
                     /locus_tag="IW728_000165"
     CDS             7033..8769
                     /locus_tag="IW728_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1023552.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8832..9668)
                     /gene="pheA"
                     /locus_tag="IW728_000166"
     CDS             complement(8832..9668)
                     /gene="pheA"
                     /locus_tag="IW728_000166"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1023553.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9665..10174)
                     /locus_tag="IW728_000167"
     CDS             complement(9665..10174)
                     /locus_tag="IW728_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1023554.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10167..11462)
                     /gene="aroA"
                     /locus_tag="IW728_000168"
     CDS             complement(10167..11462)
                     /gene="aroA"
                     /locus_tag="IW728_000168"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1023555.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11475..12566)
                     /locus_tag="IW728_000169"
     CDS             complement(11475..12566)
                     /locus_tag="IW728_000169"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1023556.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12583..13749)
                     /gene="aroC"
                     /locus_tag="IW728_000170"
     CDS             complement(12583..13749)
                     /gene="aroC"
                     /locus_tag="IW728_000170"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1023557.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13751..14815)
                     /gene="aroB"
                     /locus_tag="IW728_000171"
     CDS             complement(13751..14815)
                     /gene="aroB"
                     /locus_tag="IW728_000171"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1023558.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14848..15870)
                     /gene="aroF"
                     /locus_tag="IW728_000172"
     CDS             complement(14848..15870)
                     /gene="aroF"
                     /locus_tag="IW728_000172"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1023559.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16529..17503)
                     /locus_tag="IW728_000173"
     CDS             complement(16529..17503)
                     /locus_tag="IW728_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1023560.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17484..19367)
                     /locus_tag="IW728_000174"
     CDS             complement(17484..19367)
                     /locus_tag="IW728_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1023561.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19385..21610)
                     /locus_tag="IW728_000175"
     CDS             complement(19385..21610)
                     /locus_tag="IW728_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1023562.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21725..22759
                     /locus_tag="IW728_000176"
     CDS             21725..22759
                     /locus_tag="IW728_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1023563.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22826..23839)
                     /locus_tag="IW728_000177"
     CDS             complement(22826..23839)
                     /locus_tag="IW728_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1023564.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(23917..24105)
                     /locus_tag="IW728_000178"
     CDS             complement(23917..24105)
                     /locus_tag="IW728_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1023565.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24545..24673)
                     /locus_tag="IW728_000179"
     CDS             complement(24545..24673)
                     /locus_tag="IW728_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1023566.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24676..26049)
                     /gene="rlmD"
                     /locus_tag="IW728_000180"
     CDS             complement(24676..26049)
                     /gene="rlmD"
                     /locus_tag="IW728_000180"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1023567.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26160..27215)
                     /locus_tag="IW728_000181"
     CDS             complement(26160..27215)
                     /locus_tag="IW728_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1023568.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27225..28655)
                     /gene="gatB"
                     /locus_tag="IW728_000182"
     CDS             complement(27225..28655)
                     /gene="gatB"
                     /locus_tag="IW728_000182"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1023569.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28652..30124)
                     /gene="gatA"
                     /locus_tag="IW728_000183"
     CDS             complement(28652..30124)
                     /gene="gatA"
                     /locus_tag="IW728_000183"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1023570.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30124..30429)
                     /gene="gatC"
                     /locus_tag="IW728_000184"
     CDS             complement(30124..30429)
                     /gene="gatC"
                     /locus_tag="IW728_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1023571.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30535..32571)
                     /gene="ligA"
                     /locus_tag="IW728_000185"
     CDS             complement(30535..32571)
                     /gene="ligA"
                     /locus_tag="IW728_000185"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1023572.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32588..34822)
                     /gene="pcrA"
                     /locus_tag="IW728_000186"
     CDS             complement(32588..34822)
                     /gene="pcrA"
                     /locus_tag="IW728_000186"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1023573.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            34977..36536
                     /locus_tag="IW728_000187"
     CDS             34977..36536
                     /locus_tag="IW728_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1023574.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        10086 a         7961 c         6618 g        11942 t
ORIGIN      
        1 aaaaaagcgg aaataacgct catccgcctt cttgtttgtg tttatttcct cttcaaacgc
       61 ttgtctatga ttcagacttt tctctatcgt tattttcatc taaaagttct tgcattccga
      121 taatttctcg atattgatga atcttagtga taccaatgac tttgtctaca tcaatactaa
      181 aacaaatacc attacctgga atatcgatgt tgatgccttg accaatcgca tccattactt
      241 tattcgtcaa actatcgggt actaatgtca acacaatatc tttttctggc gaaatgtcca
      301 aacctaaaaa tttccctgaa tcatgtacac cagaacctct tccatgtaag atcgttccgc
      361 ctgctgcacc tgcttctttt gaaaagtcga taacatcttc gcccattcct ccgtcaacga
      421 tcgtcacaat catgtgaaga ttcaatgcta caagatcttt catttgcttt cccccaattt
      481 ttgcaatctt tctttttcga ttctgtcttt ttcatctttt cgtttataaa tatatcctag
      541 tactaaagtg gataatattg gtgtcaatgc aatcattgca accacaccaa acccgtcaat
      601 gatcggatct cttccttcaa tttgtgcagc gattgccacg ctcattgata aaataaatgt
      661 cgaacacatc gggcccgttg ctactccacc gttatcaaaa gccattgcta aaaagatctt
      721 gtccactttt cgtcctaaaa taaatgctag cccgtatcct gggatcaaga aataataaag
      781 agagaatccg acgagcaaac gccacataga caatcctact gctgcgccga tcccaattga
      841 aattaccatc agcatgattt tcgatcggat tctcccctcg ctcaattctt caacctgttt
      901 gatcattaca tgaatcgccg gctcagcaaa ccctactaag aaccccatta aaaagccaag
      961 gggaaccagt atataattgt aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac
     1021 cggtacataa gccatattga ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa
     1081 gaaacctttc ataatgtcaa agaaacgctt tttgcgcata cgaaaactaa tgatattcat
     1141 taaaacaaat aggattacga taggtgccac tgccaaaatg acttccatgg taacgatttc
     1201 gattccatct atcacttgct gccagaaact cattggaaga tcacccccaa aatcatcacg
     1261 gcaagaatcg gccctaacga agcaataccg accattccaa aactgtcatt ttcatttttc
     1321 ttttgtttga tcatactagt catccctccg gcaagggcca agataaatgg aacagttact
     1381 ggaccagtag tcactccccc cgaatcaaaa gcaatcggca taaattcgga actggtgaaa
     1441 aatgaggcga tcaaaacgcc tatgtagcct atagccatca attgataata ggatagcttg
     1501 aatacgaccc gaagcagcgc aaaggctaga aatactccag tgccgacgct gacgatccca
     1561 atcagcaaca ctctgccaat ctctccgtca gatatatcat atacttgttg tcccaaaact
     1621 tgcacggaag gttcagcaat cgtgatcacg atcccgatag caaatcccaa actgaccaaa
     1681 acggtcaaac ttttcttttt gatcatatat ttccctacta gatctccgac ttccatcata
     1741 gaataatctg ctccaaacaa aaagagcgtc attccgatca tcatgattgc cgcaccaacc
     1801 agaaatgaag cgaggtcttc tccttctaac ggtgcaaaaa caaacgttaa aatgacaatc
     1861 aggacagtca tcggcaatat agcagcaatc acttctttaa aattttttag catgtatcct
     1921 cctcctttac tctttaagtt taccttaaaa tgaaagagtt ttcgagcaca aacttaaaac
     1981 gttatcattt aatttttctc aacataaaat aacacacgtc ccaaaagtat ctttttactt
     2041 ggaaaaatga ctttgaagga acgcgcttat tgtgtttttt tattttacag agccgatcat
     2101 tccttgaacg aattgctttt gcaaaacaaa aaatacgatg gcagttggta aagtagcgat
     2161 gactaatcca gccatgatca ttccatagtc tggtgtatag gaagcaccca tagcagataa
     2221 aacaagcgga accgtccgtt tatcaggaga ttgcaaagct actaagggcc ataaataatt
     2281 gttccagcta ctcatgaatg tgatgattgc agccgcggca taggtatttt tagccgtcgg
     2341 catatacact ctaaaaaaaa tacttagttc ttttagaccg tctaatcgag cggcttctac
     2401 tagatctttt ggaaaagatt taacattttg tcgaaagaag aagatcaaaa aagcagtgct
     2461 aacagatggc aggatgacaa ccaagaaact gttgattccc aaaaccgttc cgttcaattg
     2521 tgaaaacatt cgatataaag ggatcatcaa tgccgcaaaa ggaaccatca tcgatagcag
     2581 tagaatattg aaaattaggt cttttcgttt actttgataa atctcaaatc catatcctgc
     2641 cattgacgaa atcagcaatg caaacacagt ggtcaccaaa gcgatcatcg ctgaattcca
     2701 aagggaacga gtaaaattca gctcattgga aaacaaattt tgaaaattga tgatcaagtt
     2761 actgccaatc tttatttttc ccgatgtgat atccacgggt gtattcgtca ttcccaaaac
     2821 catccagaca aaagggaaaa tggaaataaa tgataggatg cttaatgctg tatactttaa
     2881 tactgaccca attgtcgaac gaagttctaa tgcttgtttt ttgacgttgg ggcgttcgat
     2941 tgtttctttt tctaccatat ttttcatcga ctttctcctc ctactttcgt ttggattatc
     3001 gacaatatca caataaagga aacaataaca aaggaaactg ctgccgcata tccaaaatta
     3061 ggcgtgaatt tataactcaa attatagatg tattgagata gggtggtcgt tgcatttgct
     3121 ggtcccccgc cagtaatatt ttgtacttca tcaaaaagct gcaatgtccc gatcgtcgaa
     3181 gtaatggaag taaacaaaat gattggtttt aaatttggga tcgtcacttt gataaactgc
     3241 tgccatttag aagcaccgtc aatagatgct gcttcataaa tctccgaatc aatattttgc
     3301 atgcctgaaa ggaaaaaaat catattgtat ccagtccaac gccaagtgat cgaaagaatg
     3361 atcagaactc tcgcccaaat tggatgagtc aaccagccaa tcggtgtgtc aatcatacca
     3421 atgcccaaca acaagttgtt tacaaggcca gattgagaaa atagactttt catgatcaga
     3481 gaataactga ccatcgaagt gatacatgga agaaagatag cggttcgaaa caacccttta
     3541 tatttcaact ttttatcatt taaaaggttg gaaataaaaa gtgctagaaa caacatgatc
     3601 ggtacttgta caagtaaata caggaatgta ttaaaaaaag cttttttaaa agttgcatct
     3661 gtcaacatcc gctgataatt acctaacccg ttaaatgtca tattgacagg gggaccactt
     3721 tgaaaagacg tgatcaaagc caaaaccatc ggaataaaaa ccatgattga tatcaaaatg
     3781 actggcaaga ttaaaaaagc atgcccgttt ttgaataaat ctttctgctt cattcttttc
     3841 cctccaattc tatcattgat tatctacatg aaaagacaag aaagacaata tctgtaaggc
     3901 agacgtgatc ttttttgtct tttcgtttgt ctgatgaaaa agcgaagaaa ttcacttttt
     3961 taccaccgat caattagcca attgtgcttc tacttgtttt tgtgtatctg ctagtacctt
     4021 gtctgtctct tctccattga tgattcgatg aagagcttca gctacgactg cttcaattgc
     4081 ataggtctca tgtccgtaat tcacttctgg tatttcagat gtccatgttg aaaaatcgtc
     4141 aaagatcttt tgaccactgt aaaattcaga agcttcttga taagcttctt gttcattcgc
     4201 gctaagcatc gtagatacta gtccgatttc tttcgcaaga gtgcccatca gttcttcatt
     4261 cgaagcaaac gttgcttcta ggaaatcttt cgctgaatct tctccagcta ctcctttgat
     4321 cacataccag ccagcgccgc caaggttaga agcttgcgct ttttgcagat tttctggaag
     4381 tgcaggtatt ggtgcaatct tccattttcc agattggttt tctgctcctt ggatcgttga
     4441 agaataccaa gcgccagttg ggctagaagc tactgctcct gattgtatcg cagttactcc
     4501 agcattccag tcagaggttt gttcgactag atcttctttc aaaagagtgg caaacaattc
     4561 aagaccatac tttaaagatg cattgtcttg gatcgtgact gtctccccat cttcagatgt
     4621 ataccattct cctgcctgtt gcataatcat ccttactcga ccaaggtcag aaggattgac
     4681 ttcagcgatt ttctttcccg tcttttcttt gacatctcgc gctacttgaa tatactcgtc
     4741 ccacttcaga ttctccatat cctcttctgt ataacctgct tctgccatca gatccgtacg
     4801 atagaagttt gctgttacgc ccgaatcgaa aggaacaccg tatattttat ctcctacttt
     4861 gtttaccgca aatttatacg gtgcaaaatg atcttccttt acaatagcag acaaattagc
     4921 gaatgcatct ggataagaag tcaaatagcc ttgtatccta taatcttcaa tcaagacgat
     4981 attgggcaat ccttctgtat tcccgctcgc taaagcggtg ttcagtttct gaacgatatc
     5041 atcctgagac atcgtaacaa cttctatctc tgtttcttcg ttctcataaa ctttttttgc
     5101 ttcattcact gctttgatat tgaatgtctc atcccacgcc cagacagtga ctttttcttc
     5161 tttgtctgct gtactactag cacttccgca tcctgttaag aaacctgatg ctgccgctaa
     5221 acaaacgact ccagcaatta actttctgat gttcattttt atccgctcct tttccatcca
     5281 ttagttgttt acgtttgcta cattcatcat accaaaattt gaaaacggtt tctttattat
     5341 tcagatactt caaaaatttc tcctaaaata ataaaaaaat acaaatcaac tatgacaaaa
     5401 ggaataatct tagcatctag accaaagatc gaaatggtga ggtttcttta aaataaaaat
     5461 atccttgcat tttctcctag aaataagtat ttttaaatta caagactatt cttaaggcgg
     5521 gtaaaaaaat gccaatacgc gtaattctta tcgatgatga acagacgatt ttagatggaa
     5581 tgaaacagtt gatcaattgg tctgagcacg ggtttcaatt aatagggact ttttccagtc
     5641 cgctgaaagc gcttgatttt tctttggtac atgcaatcga tatcgtggtc acagatgtaa
     5701 tgatgcctga attaaatgga atcgaattat cgcggctatt aaaacagcaa caacctgaaa
     5761 cacagatcct tatcctctct agctatgatg aatttgatat ggttaaagat tcttttaaag
     5821 aaggcgtcgc tgattatctt ttaaagcctc gattgagtcc tgattttttt ctaaattcct
     5881 taactgccgt ttcacaaaaa atcaaaaaaa agcccaacaa aagcccaatc accaatcgtt
     5941 atgagcagat ctgcgagaat ctttcacagc agattgctgg acaaactcgt ccattgcttg
     6001 accctcttct ttttgaacac accttgtttt tctgttatat accgaaaaac ggccattcag
     6061 ttcaaaacaa ggctctaaac aaaaacagtc gatcacccat tcattgatgg atagcgacga
     6121 ttcgtttact atttatcctg tttctacctt ttcggaagaa tccggctatg taatcaatac
     6181 tgattccaaa gatacactta ggaagtttgt tcaaaagttt cgtcaagaag tttttccagg
     6241 tgattttttt gttgtttctg attcttttgc aatccaagat atcaatgaaa tattccaaga
     6301 gatcagaaat tgcaccaaag gacaacgttt ctatcataag ggaaagaacc ttgcttatca
     6361 gcatgaactt cttccgttat taaccaacca cgagcagcaa gcaagtagtt atttaacaaa
     6421 aatcgttcat aaagactacg ctttagctgt caaagaactt gagaatttct tcacgacgat
     6481 attggaacag cctattgatc cagcttattt gaaacatgag gcaatcaatc gtttctatgc
     6541 attactcaac gctttggctg acgagcatgt tgggaatgaa gaactttctt tgctcaagat
     6601 cgcccttcct tctttattag ccgatgctga gtatttcgaa gatttttcta ttcttgtcaa
     6661 agaaacactt gagaaactca cccacttttc tgcaaatgca aaaaaagaca atttcgatat
     6721 cttgactttg atctacgagt tcgtccagga aaattatgag aaagaaatca atttacaact
     6781 tttatccgaa aaatatcatt tttcatatag ctatctctca gccatcttta ctgaaaaatt
     6841 tggtatcaac ttttcaaaat atctaaaaaa agtccgtatc agtaaagcca agctcttttt
     6901 gacggaaaca cggcttactt tgacggaaat ctgtgagaaa gtcggctata ccgaactggg
     6961 ttatttctcc agagttttta aagaagagac aggaatgaca ccttctcaat accgtaaagg
     7021 aacttttgta aaatgagaaa aaaaatcaaa gagcatgaac tctttgccaa acttctgttc
     7081 attttgttca tcggtctttt tctgcaagcc atcgtcattt ctttgtttat ttatcatcga
     7141 tcaagggatg cctatatcca gctgttcaat caatcgaatg atgtggtact aaaaaagatt
     7201 caatctgaat ttgaaagttt gaatgataca atcgagaata cattagccgc ttttgattct
     7261 aatccagctg tcaaaagcta tttttccaat gatccagccc aacatatgga acagcttcag
     7321 caactaagaa caatccaaaa aatgaatgac tctctttcaa aaatccatcc aatgatcgat
     7381 tacgatgtat tgatctttgg agaaaatgga agaacttttg tcggaaacga catgctcact
     7441 gccgtatctg ccgattcttt ttttcaatct gcgattgctc agagagtgaa tgaacgtgct
     7501 gctgatacac aaatgctttt tgcacaccac ggattgactc ttcgcgataa gaaagcaccc
     7561 agcgtcttct ttgtcaaaaa actgaaaaat acattgaatc acgtttacgg atatgctgtt
     7621 ttatcgatct catccaagca actagcgaat ctttttcaat ctgtagtaaa tcctgagata
     7681 tcgaagatct ccttcatgaa cgaagaacag acgatcatcg catcaaatga agaaaataca
     7741 attgggagcc gatcaatgat tttcaaaaaa ctaacagtag gtgaaacaac gatttcaaat
     7801 ggacagcagt tgacccgtct tcctctgtac cgccaggatt gtacattact cagtcaagtc
     7861 gatcttcatt ctttatctaa ccaaatgggc gtgatttttc cgattatttt gtataatatc
     7921 gtgacacttg cttttgctgg ctcattggta tttatgtatt tggatcgaca cacaaaatcg
     7981 atctaccgct tgattgattc gctaaaacgg ctagaggaag atggcttaaa agctactgtt
     8041 cctgtacaag gaagccatga agtccaattg ctagggaata cgatcaatca attgctaagt
     8101 aatgctcagc atcattatga acagacgatc caagacgaaa agaagaaacg ttcgcttgaa
     8161 atccaaacta tgcaagcaca gatacagccc cattttattt ataatacgct gactagcttg
     8221 aaatttttga tttggcagaa aaaaacagat gaagcagttc aaggaatcga cagtttcatt
     8281 actttactaa gaagtacgat tgggaaaaaa gaagaagtga ttccagtcaa ggaagaaatc
     8341 aaaagcgtac aaagttacat cgatcttttg agtatgcgat atggcgaaag aatctcggct
     8401 aaaataatga ttcctgatga actgcagtca ttatctatgc cgaatatgat ccttcaacca
     8461 attatcgaaa acgcctatct tcatgctttt cacaataaaa cgcaaggatt catcacggtg
     8521 tacggcaaaa caaaaaatca gcttctttta ttcgaagtga ttgataacgg cgatggcttc
     8581 gatactgaaa ataccaaaca aaaaaatgat catttttcag gaatcggcat cagccatgta
     8641 gatgagcgga tacgcctaat gtatggcgat gcatatggct taacgatcca atcagtgatt
     8701 gggacaggaa cgactgtaac gatttgtcta ccgcttcttc ctaaaaatca atcagaatat
     8761 caaaaataaa gaatagccaa gcgctgattt ttttatttta agttaaataa atcaatcagt
     8821 attcttgact ttcatctacg cgtctcttct actgtcagca ctggataaca gcccaatatt
     8881 tgactttctc ctccgagcat cttgatttct tggagcgcat tctctactaa tgtcattgga
     8941 cggtccaaca acaaatcgat cacgaaaaaa tattctccca agctagtttt caatggtcgg
     9001 gattcaatct tacttaagtt gatttcccgc cagccaaaag ctgccagcat tttatgaagc
     9061 atccctggcc gattagcagg tagtgtcaag atgacactca tttttactgg agctgactgc
     9121 tggctggtca tctttcggtc accgacgatc caaaaacgtg tctggttcaa ctcattatct
     9181 tggatattct cagctaagat ctccaatcca acatgctgtg ctgtttctaa tgaagcaatc
     9241 gctgctgcgt cttctttcgg atgttctgct acatacattg ctgcagcagt tgttgattct
     9301 gttgctacta aaggaacatt aggataatgc gtttctaaaa actgttgtga ttgtgctagt
     9361 gcttgtggat gtgaaaggat ttttgtaatc tttgttgctg gattgcctaa aagctgctgc
     9421 ttgataggaa gaacgatttc acttttaact tcgacttctg gatgtttgga aagaagatcg
     9481 attgtgtgat gaacagctcc ttccaatgaa ttctctacag gaacgacagc cagatcgact
     9541 tgtttgcgaa acaaagcgcg caaacaaaca gggatggaag catacgcagt caattgttct
     9601 gtaggaaata gctgacaagc cgcctgataa gtgaatgaac tttctggccc tagatagctg
     9661 acgttcatat gcttttgatc ctttctataa tggtatcgat gatcttttct ggtgtttgat
     9721 tggtcgtgtt gattcgatgt gtcgcacttt cttcatacca agaaagtcgt tgtgccaata
     9781 atgcttttat ttcttctctt gtttttccaa gagctaaagg tcgaatagac gtttcatctt
     9841 gttggatacg atcaactaga cagtccgctt ctgcctctaa gtaaagaaca atcggctgtg
     9901 tttttaaaaa acgccgattt tctgcacttt ggacaatccc tccaccagtc gcgataattt
     9961 tgtcttcatt tgacagcttt cttaaaatat ctgactccac ttttcgaaag cttttttcac
    10021 cgtaatagtg aaaatattcg gcaatcgaca attggatatg ttcctcaatc tttgtatcta
    10081 aatctacata ctctttttgt aaacattgag ctaacccttt accgattgtt gtttttccag
    10141 cgcccatgaa accaatcagc aaaatgctag ccatgagctt ctcctcctga cacaagttct
    10201 gctaaatcgt caaaaaatgc cggataagag attgaaacag cttcagctcc ttctagttcg
    10261 caaggtccat cagctatcaa agcagctact tgcagcatca taccgattcg atgatcgcct
    10321 ctgctggaca cttttgcgcc atgcagaggt gtcggcccat ggatgatcag accgtctgct
    10381 gtcgctttta tctttgctcc cattttctgt aattcttctg ccaccgcatc aattctgttg
    10441 gtttctttta cttttaattc ttctgcgtct ttgatgatcg tgattccttc tgcttgagta
    10501 gccactaaag ctagaatggg caattcatca atcagacgcg gaatgatttc tccaccaatc
    10561 gttgctttct tcaaatgact cgtcttgaca cttaaatcag ccgattggtt ctgttcattg
    10621 tgattcgtct gggtaatcgc tgcgcccatc tcctccaaca catctaagat accagttctt
    10681 gttggattga ccccgacatt ttttaacaac agttggcttt ccggaacaag aagtcctgct
    10741 gctagaaaga aagcagctga tgaaatatca cctggaacag ttatctgttg accagttaat
    10801 ttttgcggtc cggtcactat gatcgtttta tcttccactg tgatccttcc gccaaattga
    10861 cgaatcattt cttccgtatg gttacgggaa cgttcttttt caacgattac ggatgtgcct
    10921 tctgcttgta atgccgcaaa cagcaaagca gatttgactt gcgcactggc tacaggcata
    10981 ttgtatcgga taggtgataa agagcgggtt cctgtcacag tgattggtag atactggtca
    11041 ttttcttctc cttgcaagtc agcgcccatc tcgcgtaaag gttccatcac tcgccccatt
    11101 ggacgtttag acaacgaagc atccccgaac agagtagtcg taaaaggctg tccagccaaa
    11161 atacccatca ataaacgtat ggtcgtaccg gagtttccca tgtcaattga ttcggttggt
    11221 tggacaaatg agtggctacc gcgaccatga atcttgatca cctcttcctc ttcttcgatc
    11281 tcgacaccta attgttggaa ggcatgtaaa gtactcagac aatcttctgc gcgcaaaaaa
    11341 ttttgtatag tcgtcgttcc ttcagcaatt gctccaaaca tgatgctgcg atgggaaatt
    11401 gatttatctg ctggtatcct aacagtccct cttaatccat gtatttgttg taataattgc
    11461 atttatccag cctcctattt gatccagcaa tgataactgg tattagacat aatgattctt
    11521 tttgcttgta acaaatcctg ttggttttta aaactgattt gcagtattcc taaaatatct
    11581 tccctggtct cttggatctt caaattaatc aaagaaactc ccgcttttcc gatcagtcca
    11641 gttacttctg caataacgcc aggttcatct ggcacatcga taaataaatc aaaaaaggca
    11701 ggaatcgcgc cttgcttatg gacaggcaac tggtctcgac tttcttttgc ccgattgaaa
    11761 aaagaaaaga tttccgattg gttctcagtc atgatccatt ctgctatttg tgtcatttct
    11821 tgttgccaat ctgccaatcg ttccaataat atctgcttat tgctcaacaa aatatctgtc
    11881 cacatgacag gatctgagga agcgatccta gtgatatctc gaaagcctcc tgctgccagt
    11941 tgtttggctc gcggatactc ttgattgaat tgttctgcct gattgactaa gccagctgcc
    12001 agaatatgtg gaaaatgact tagcattcct gttatacggt catgttctct tgctgtaaga
    12061 gttacatatt ttgctttggt tccgcaaaat agttcttgca gtttttcaac atcttttttc
    12121 gtcttttcca aaggtgtaaa gatgtaatag gcattttcaa atagtttagc atctgctgcc
    12181 tgtatacctg atttatgaga accagccatt ggatgtccac cgacaaaccg aagatttttc
    12241 tgctcagcgg atgtcacgac ttgctgcttc gtactgctga catccgtaat gatgacgtga
    12301 tcagacaagt tgattctttc aaggacatca agataagcta aggttgtacg gacgggaacg
    12361 gctaataaga taacgtctgc ttgttctgca cctgtttcaa aatcacttgc aacgtaatca
    12421 acgatctgct gttctactgc ttgctcacgg gtttttgact gactatccca accaactact
    12481 atcagttctg gattggtctg acgaatacaa agtgccatag aagcaccaat cagtccaagc
    12541 cccacgataa aaacatgttg tgccatctta tttgccccct ttttagtaat gtttcaagta
    12601 gtcacggtac tccttcactg cagctttcaa ctcaacaagc gaatcacttg ggaattttga
    12661 caatagctct gtcgctacca cagtggctac aacattttcc gcaacgacgc tagctgcagg
    12721 aactgctgta ctatccgaac gttctacact tgctttgtaa ggttcttttg tatcgatatc
    12781 aacactttga agtggcttgt acaatgtagg aatgggtttc atgaccccgc gaatgacaag
    12841 tggttcgcca ttggtcattc cgccttcgaa gccgcctaga ttattggtac gtcggaaata
    12901 gcctctttct tcttcccata ggatctcatc catcacttct gagcctggta atgaaccaga
    12961 agtgaatccg accccaaatt ctacgccttt aaaggcattg atactaacca cagcttgtgc
    13021 gattttggca tctagcttcg tgtcccattg tacgtagctt cctaatccag ctggaacgcc
    13081 tccaacgagc acttctacaa ttcctccgat cgtatctcca tttttctttg tctgatcgat
    13141 caaatctttt attctttgct caactgctgg atcaacaact cgtacttctg acgcttcaga
    13201 aatttctttg atttcttgaa tcgagatatt tccaggtatt tctgcttgga ttccaccaag
    13261 acaaatcacg tgtcctgcta cgatgatgtc aagttcttcc agaattttct tggcaattgc
    13321 gccgattgct acacgcatgg tcgtctctct tgcagaggac cgttcaagca cattccgaag
    13381 atctcgatga tggtatttca taccgcctac caaatccgcg tgtcctggac gcggacgtgc
    13441 aactcgccgc agtgattggt ctttttcagg cacttctgct acagacatga cagttgtcca
    13501 atttttccaa tctttatttt ctaccactaa agtgacaggt gaaccaattg ttttgccgtg
    13561 acggatgcca gacgtgatcc gaacacgatc tttttcgatc ttcatccttc ctcctctgcc
    13621 gtaaccagct tgtctgcgag cgagttcgtg attgatttct tcttctgaca gaggtaatcc
    13681 tgctggtagt ccttcaataa tggctgttaa ttctggtcca tgggattctc ccgcagtcat
    13741 ataacgcatc ttacagtcct cctaagtaat ctttgaatgt ctctgtggga acaggaacga
    13801 tttttgcttg cccaatcttt tctagcaaga tgatattcaa ttggcttcct cttgctttct
    13861 tatcatgggt tatcgcgtgg aataattcat ctggatgact tagatcgtac gtaatcggca
    13921 gatggaattt ttgaagtatc ctttctaaag cagctgtcga tccagttgga gacaagccga
    13981 ttttttctgc atgagaagtg atggctacca ttccaatagc tactccttct ccatgactga
    14041 ctaccccata tcctgctgta ttttcaattg catggccaat tgtatgacca aaatttaaaa
    14101 taagtcgatt tccttggtca aagacatctt cttccactac ttttcgtttg atttccaacg
    14161 tagcagtaat gattggaaca gcatgttcca gaagatcttt ttcgtctttc agctcgttca
    14221 gctgtttcca aagcgttgca tctgctatag ctgctgattt gatgatttca gcgattcctt
    14281 ctcgtaaacg acgtaccggc aaagttttta gaacctcagg atcaatcaat acaccagctg
    14341 gctggctaaa tgttccgaca aggtttttcg ctttgtttgt attgactgct gtcttcccgc
    14401 caatactaga atctacttgt gcaagtagcg tagtcggaat ttgaacgaaa tggatcccac
    14461 gcatgaacgt cgaggccacg aatgctgcta gatcacccac cattcctcca ccaagtgcaa
    14521 gtatcccatc gctgcgggta aaatcattat ctgctaaaaa atcgtataac tcagctgctt
    14581 gttctaaaga tttactggtc tcacccgccg gtacgattga aaggactggt tcaaaattag
    14641 cttgtctcaa ttggaacatg attttttctg catatagtgg agcaacgttt gtatcagtga
    14701 tcacagctac tttttgaggt ctccaaatcg tttgaagcca tgatccagcc tgttctagtg
    14761 cgcctttttc aatatgaata tcgtattttt ggttatcaac atgtacagta agcattcgtt
    14821 tcacttcttt cttttaaaaa gctggaatta atcgagtgtc tgaatctttt ccattgcttc
    14881 tttcatgatc gcaacttctt tcatcatgcg tgagtaagtc ttttcattca acgattgggg
    14941 accgtctgac caagcatttg caggatcagg atggatctct acgatcatgc catctgctcc
    15001 gcttgccaca cccgcacgag ccattggtgg tacaagttcc caaattcctg taccatgact
    15061 tggatcaaca atcacaggga aatgagttaa ttttttcatc aatgggacag cacttaaatc
    15121 aaacgtatta cgtgtcgctg tttcgtaagt ccgaatccca cgttcgataa aaatgacagg
    15181 agatttgtct tcgactgcaa tgtattctgc agcattaagc cattcattga tcgtaccgga
    15241 aattccacgt ttcaatccaa caggtttacc tgttttaccg acagcagata gcagtttaaa
    15301 attttgcata ttacgtgcgc caatctgtag gatatcgctg tactcagcca ccatatcgat
    15361 atgcccttca tccatcactt cagtaatcac tttcattcca aattcatcag ccgcttgacg
    15421 gatgtatttc aatccttctt cttctaaacc ttggaaagca tatggagaag tacgtggttt
    15481 gaaggcacca cctcgcagaa tttttgcgcc cccggctttg gccattcgcg cacattctct
    15541 gatttggtct aatccttcga tagaacaagg accagccatc gtcacgaaac tgccgtcacc
    15601 aatttttact ccgtctacat ccacgactgt attgccagga tggaattctc gagaagtcaa
    15661 tttgtatgtg tttaaaatac gcacagcatt ttctactcct tcatagcgtg taaatgctcc
    15721 ttcttgtaaa cttcgcgtat caccttttag tccaatcact actcgttctg ttccttgatc
    15781 gattgagact ttgaatcctt ctttttcgat atgatcgacg acttttttta tttgttcttc
    15841 cgttgcttct tttttcataa ttactatcat tgtttttccc gctttctttt gtttacttat
    15901 ctatcttggc tgattttctc tactttttca aggtggtttt acattaattc ctactaaatg
    15961 accgtgtcct gttgttctca ttttcgctcc ttctttccta cttatgaccc agataatcaa
    16021 caaaaaaaga tgtccctgta ggaaattgcc tctacagtga acatcttcaa cgagccttaa
    16081 aaagcgctgc aaaaatataa aatagccact atagatgaat ttctctacgt ggctgtaagt
    16141 cgtgtttaaa aatctttagc catcatagat acaaacctgg tttgtttgta cccacgaagt
    16201 catgaataca aacctatcta aaatagctaa agtaatacgt attcagtttt gtaagttttc
    16261 tagatttcat aaaaaatcgc tccaaactga tgtaagaatt gtacctagta tagacataaa
    16321 aaaatcgact gtcaatcatt tttatgaaac tttttttaat tttctgatag ttaactttgt
    16381 ttttcagaaa aataacaatt ggttttattg aagaaaatag tcatgaacaa aagtatctga
    16441 gaactttttt actctcttgt tcatgacata tagaataaag cagctttgtc ttgtaaactc
    16501 cgtttttcaa ataataatct aaagattgct actgaataat aaagccgatt tcatggtcct
    16561 cttcgctgct gattcggtaa ggtgactcaa catccgctcc ccagctattg atacccccga
    16621 ctcctcttac ttttgcaaaa atcgtaagaa ccgttcgtct ttctggcggt agttcttcta
    16681 tatgtgttgc ctgttccagt tcttctgctt tgaatggtaa agcggaaaaa gcaaatggcg
    16741 tatcctgctg ttcaaatcgc aacgaaaaat cttcttgatc tttatccgta ttatccaacg
    16801 tttgatttct tgttagttct acccatttcg tttccatatg catgccgcat tcttggggaa
    16861 caaggtatgg ggaaacgggg agtcctgcca ctttataaat accttcgaca ccaccagcca
    16921 tccgatccgg atatgtttcc cctgataggc cttcatactc aaaaaatttt gctttcgttg
    16981 gcataatgaa ccttaaaccc aatgcgggta attccggtaa tcctttttgt ccataataat
    17041 gcatattgac aagcatttta ccctctccag cgatttggta ggaaattgtc acttgggttt
    17101 ttggtactgt gttgatttcc agtgtaaaaa tgatttcaac cgtttcagcg tattcattac
    17161 ttgaatattg attatttact ggagcaatcg gcaaatcgat ctttttattg tccaccagta
    17221 cttctatttc tttgactttt ggatacaatc cggctcctaa ccacataccg gatttaaatc
    17281 caaatccgct acctctgtca ttatcggtcg ttgctcgcca aaaaactgga agtgtttccc
    17341 ggtatagcca ttcttttccg tttttattga gcgattccag tccgcctctt gtataagaaa
    17401 aaatataatg cgtattcccc gatttgacac ctaatgtaca gtcgccgaag acgattgcaa
    17461 ctttttccgt atccatatga cttttattta ctgtattttt cataataata tcttacctcc
    17521 tgcatggcgg gtttttcttg tccatctgca aaaacgatcc cattccctga gaattcatag
    17581 tcacttggtt tatcgtcaaa gtcaccgcca tagcgcatca cttctctgcc tgtgattgga
    17641 tcagtcactt ggatcgcctg atctttaaaa tcccatatat agcctccttg gtacatttca
    17701 taacgatcaa gcaacgtcat gtaagaatcc attccaccta acgaattccc catgtcatgc
    17761 atatattcac ataagatgaa cggtttttcc gctttatttt caaggtagtc aatgatctct
    17821 tctggtggcg catacatgca gctttttaca tctgaaatcg aatcttcgaa tgctggattt
    17881 ggaaaattcc cttcataatg gacaagtcga tcggggtcta cttttttgaa gtaatcgttc
    17941 atttgtcgaa tgttttcttc cgcataagat tcatttccaa gagaccaaaa aagaatactt
    18001 ggatggtttt ttaatgtttc gaaattcgtt tttgctcgat caagaacagc cgcctgccac
    18061 tgaggtactg cacctggtac gttgtaagaa ggctcaaatg cgcccatttt ttgccatgag
    18121 ccatgggatt cgaggttcgt ttccgccatc acatggattc ctgcttggtc gcataaataa
    18181 taccaagtga tctggttggg gtaatgagat gtccgtacgc cgttgatctt tgcatttttt
    18241 atgatagcca aatctttttc catatcttct atagtgatgg cgcgtccttt tttcggatgc
    18301 cactcatgtc gatttacccc gttgataatc atccgttttc cattgaagta gatgatttta
    18361 tctttgattt cgattttacg aaaaccgaag gaatagtcaa tatactccgt tactcttttt
    18421 tcttcgtcta tcgtctccaa ttcaagctta tataaattgg gctgaaaatg cccccacagt
    18481 gccacatttt caaatccatc gatgcgaatc gtcatttgct cttttaacgg atattgctgt
    18541 tgatagattt ccttcccatc aggatctagt atcgctacat tcagctgact gtttttggct
    18601 acttgctcaa aagacaaatc tacttcaagt ttgccatttt gacatttgtc atctaagcga
    18661 ggtttgatcc aaagatcttc taaatggttt tgtcctcgag caactaagaa gacatctctg
    18721 aagatcccaa aaaatcggaa aaagtcctga tcttccaaga atgcagctgt acttcttttg
    18781 taaacctcca cagctagaat attttcttct tccaaataag gtgttaaatc aaattcagac
    18841 ggagtgaacg aatcttcggc atacccgata aaatgaccat tcaaccaaat aaacattgct
    18901 tgttctacac cagcaaaatg aatcgagatt tgttttccct ttagaccttc ttctaatcga
    18961 aaagtcgtca aatacgaacc aaccggattg tctttagcag ctgaaaagat cccttccgcc
    19021 tctttctcag ggcttgtcgt gaaaggggga cggcgaaata catgtccctc ccacggatac
    19081 atcgtattga tgtaatggat cttgtcataa tttgctaatt caatatgact aggaacagtg
    19141 atttcatcaa aagaacaggc atcaaaatct tttttataaa atgtttttgg tcgtaccgaa
    19201 ggatttggcg aataacaaaa acgccatgat ccatttaaag actgtttaag ggaggatgtg
    19261 gtttctactt cttcccttgt tgcataacaa acatgatcgc tatgtgcatt tgattggttc
    19321 actctaaaaa cttgtggatc atctaaccat tttatatttg ctttcatact tttttccttc
    19381 tctctcataa ctttttgaaa tgaagcagtt ctgtttggaa atcaccttga aaatcgggga
    19441 agtaaagtcc aactgtcatc aattcggccc ccgaaaatat ccgcttcgag gtctgttcct
    19501 gataaagtgc ctcatcatct aaccccatca gatatacttc atggaacgct ggttgagctg
    19561 aagctagtat cctccctaaa aacacaatag cttcatcttg tttaggagac acaaacatcc
    19621 atgcggcaat attttcttca aaaggactcg ccaaacgata ataatggcca tactggatca
    19681 ccggacgaat cgtttgatag gatataattt ggtcttttac ttgcttttct tcttcaggag
    19741 acagctttgt caaatccagt tcataaccaa aaacagcgct catagctgtt tctcctcttg
    19801 ttttcaacga agtagatctg tcggtctgat gatttggtac agctgataca tgagctgtga
    19861 tggacgaaat cggataagca aggcttgttc catattggat tttcatacgc tccactgcat
    19921 ccgtgttatc acttgtccat gactgcggca tataataaat aaacccggca tcaaaccgtc
    19981 cacctccacc agaacagcct tcccataaga tttccggata atcagttgtc aaatgttcaa
    20041 gtagttcata aagcccgagc atataacgat gcgctacttc tccttgtctc tgtgggtcaa
    20101 gggtaatcga atagacatct gaaagcgaac ggttcatatc ccatttgatg tagtcaagag
    20161 gaatcgtatc taaaatcttt ctcatttgtt tctcaatcgt ctgccgaaca tctaaccttg
    20221 taaaatccag tacatgttgt gatctggaag cagaaggcat tctacctggc tcctgcataa
    20281 gaaaatcagg atgtgttcga tacagttcag aatcaaccga gatcatttca ggttcaaacc
    20341 ataaaccaaa tttcaatcct ttgctatgag cataatctgc tatttcacgt aatccatttg
    20401 tcaacttgcc ttcatactcg aaccagtctc ctaatgaaga attgtccgca tctctttttc
    20461 caaaccagcc atcatctaat acgaacattt cgatgcctag ctcctttgct tcatcaatga
    20521 tcgccttgat tttttcacta ttgaagtcaa agtaagtggc ttcccagtta ttgaccagaa
    20581 tcggccgttc agcaaattga tgacgacctc ttgcgattct ttcacgtagc aactcatggt
    20641 ggatctgact cattttgttc aatccctgac ttgagtaaga aaggatcatt tcaggtgttt
    20701 gaaaagactg gcctgcattc aattcccacg aaaaacgatg gctgttgatc ccaccgacga
    20761 ctctcaattg attgatctga tctttttcaa gttcgaaaga atggttgccg gaataaacaa
    20821 attgaagtcc gatcgcttct ccagtatttt ctgtcgtatg atgatgaact aaagcaataa
    20881 agttattcat atggtgactg ctcgttcctc gacggctttc aaatactttt gttccagata
    20941 aaacagattg ccgtgaaatc tgtctttcat tgacatgtgc tcctggcaaa gcaatgactt
    21001 catcaaatcg cctagtatgg gcaaagtcta gctggaaact agccgctttt tctagtttga
    21061 ttgtttctcc ggtttcattt cttatcttta cagatcgtgt gatcactgct ctatcagtaa
    21121 atatggtata gaacaaatcg aaataaatcg ctgtttctct atccttaagt gaaatcacca
    21181 acgtttgtgc ctctgacttg tccatcacat aagtttgagg caagccttcg atctctggct
    21241 tcccatctgt aatataatgg ctgtcatatc tcagatccag taagtctgat ccattcttgc
    21301 gctgaatgat cgatgcgggc gttcgaaagt ccatgctgcc atgagaagag tattcttgcg
    21361 gtagtgtatc tttcgacaga gaccggtcag gatttaaggg aacatttcct gaaaatccac
    21421 gatctcttcg gggataattt ttattgtctt tgtactgctt gactggtttg ccaaaataaa
    21481 tatgcgccaa tacattgctt tcttctatct ttatcagata agaaagaaat gtatttcgta
    21541 aatggaagac tcggctcttt tcatcaaata agatgaaatt tttatctgtt tttctatgcg
    21601 taatatccat catttcactc ccaacaaaac ggaaaccgtt tacttttttt tataattcct
    21661 gtatactgtt tactaaacaa ttttgtcaat aaaaattttt tatttttttg aggagtgtgt
    21721 aaaaatggtt ggaattagag atgtcgcacg aaaggcaaat gtctctcctg ctacagtttc
    21781 ccgtgtgttg aataacgatc agacgatcca tgtggctcag actactcggc aacggatagt
    21841 agaggcagct gctgcgttgg attatgacat cagtactcgt aaatatatca aaaagagaat
    21901 gccttcgatt ggtgtgatct ctacgatcgg caaagcgagc gaagaacagg atatctatta
    21961 caaacatttg cgagtaggga tggaagaaga agctcgacgt ctgcatttag ggatgaatcg
    22021 aatctataat ctatctgata atcctaaaca atgggaagat ctagatcagt taggtgcggt
    22081 gatcgttgtt gggactgtta cgaaagaatc gattaccgcc ttgttaaaac aaaacatgca
    22141 aatagtcgtc gtggataacc ctgacatcca gcaagaggtc gatatggtgt atgcagatct
    22201 tgaacggatg actaaaaccg tactggaatt gttcttagaa aatggtcata aaagaatagc
    22261 ctatatcggg ggatataaag tcgatatgga tgaattcggt gggaagcaga tttctataaa
    22321 tgaaaagagg atgcgtgcct atcagcattt tatgatcgat catcacttag aaaaatatat
    22381 gtcttatcat ctaggtggct ggacagaaaa acaaggtgag gtacttgcga ctaatctatt
    22441 agaaaatgaa tcccctcttc ctactgccat tcttgtcggg agcgatccaa tggcaattgg
    22501 tgtctatcgg gcattgaaac aaaaaaatat ccaaattgga gaagagattg ttgtagcaag
    22561 ctttgataat atcgagacaa tcgcaaacct ctcccctggc ttgaccagtg ttcaaatcaa
    22621 tgcgaaatca attgggcaag cagctgttcg aatggcattg gagcgaattg acggtatacg
    22681 ggacgaacca atcattttga cttatcctgc acgattgatt gttagggaaa gttttatccc
    22741 aagaacaaaa aggtattgaa agagaggttg tgaaaagccg cttcgtttct tcatactcaa
    22801 aacgaaacgg ctttttcaca acctcttact tatacttatt cacaaaatcc aatgtctgct
    22861 ggtaacaatt ttgcgattct tttagcgtgt aatcaaattg gtattcatgg gtaatctctt
    22921 tttttgtatc tgtataaaac aaggattgca cgggtacctt taattctgtc aaccgatttt
    22981 ctaatgcttg accttgctcc tggaatgagt atgcatttcc gtcagttata tacgttggcg
    23041 gaaattcctt agaaacatgg tcagccacac tagcttcttt caattgctca ctcgttttcc
    23101 aatcttttgt tccaatcaac gaccacgcaa cggttttgac gaaaaacttc ataaatcgat
    23161 catccgaatg ctgctgtgct gttagcttca aatcaacagg tccgcaataa gaaatggttc
    23221 ctttcagact atcttttgga agtagatgtt tgattttcat ttgtttagca tacgcatcat
    23281 tcgtttgtat agctgcatat tgcaatgcga tttgtgaacc tgcactatct ccaccaaaaa
    23341 ataattgttc catgtctagc aatttgtcgt ctttttcttc agctaataaa tctttgatca
    23401 gttcattcac ttgaagaact tgattagggt attctgaatc aggcgctaat tcataattca
    23461 tcgcaaccac tgcgatgttg gcgtctgaaa ctaattttgt cgcaaattct ttgacacccg
    23521 atttatctcc gccgacgaat cctccaccat gtacccaaaa tagaacagga acaggcccct
    23581 tgctgttttt tggatagtag atgtcataag tattctcttt atacttggac tcatacgtca
    23641 aatctgtttt tttcgccacg tttttttctg cttgatcgaa cgttttttta tcagtgattt
    23701 gaacagtatt tgaaaacata tggttgatca agaatgctcc aggtctgggg gaaagctgga
    23761 aggcaagaaa gattgcaaac gcgatcccaa gaatgattcc gaagaaccat ccgataaatt
    23821 taagaatttt ctttttcata aggctccctc agtagttagt attaagcatt ctgattatac
    23881 catagtgtta tttcctatca agctctccta gatgatttaa tgattttccc caaacttctt
    23941 ctgcaatgct tttgacaaaa gaaatttttt ccattgttct tcaaacaaaa cattttcttc
    24001 ttctgttgaa gcaaatccac attgaggact taagcacaaa cgatccaaag gcagatatcg
    24061 tgtcgcttcg tagatccggt caatcacttg ctggtgctct tccatttcgc catttttcgt
    24121 tacagtcaaa atctaagaca actaatttat cttcagagac tttctctaaa agaacaaaac
    24181 tcccagcacg ttcactatcg tattctaaat aataggcacg gacattttct tgagtaaaat
    24241 aatgggtctg ctacgtcgtc atcccccctg atgccgccaa aatcgaataa taatttccgc
    24301 gtcaaacatg tatatttagt gttgaatcat ttgagaatat gaaaataaaa atctttttga
    24361 tcaatcagtt aatcaaattg aaataacgaa aaatctgaga cagaatgacg ttcattctga
    24421 cccagatttg attttgcaca actcaacagt ccttattggc aaatcgccaa aataatgtag
    24481 actaactaaa aagactaaga aacaataaca aaagttaaca cttcttagtc tttctttagc
    24541 acgtctattt attaaagatt ttagggcctt ttctcatgtt tttacgatca ttcttaggtg
    24601 tttccatttg tttggatcgg cctccacctt gtttcttttt gatcaattct ttcatttttt
    24661 ctttttggtc catttttatt ctcctttaac gaatgaagcg actaattcga tatgatgggt
    24721 ttgcgggaaa agatctacag gctgaacttt cttcacggca taacctgctt ctgcaaataa
    24781 cttagcatct cttgcaaaag ttgccggatt acatgagata tagacgattc gttctggtga
    24841 catttcgatt gctgattcaa taaaatgatg atctaatcct tttcgtggag ggtcaactac
    24901 gatcacagat ggtttgatcc ctttttcttg ccattttttc ataacttttt ctgcttttcc
    24961 gacttcataa tgaacatttt cgattttatt atttaaagca ttgaactgtg catcttcaat
    25021 tgcttctgga ataacttcca tcccatacac cttatccact ctgtctgcta aagacaagcc
    25081 aatcgtacct attcctgaat aagcgtcgat cacaatgtct tttttatcta actcggcaaa
    25141 ctcgatggct gtttggtaca gatactctgc ttgttgagta tttacttgat aaaatgactt
    25201 tgctgagatt cgataggttt ttcctaatag ctgatcttcg atatagtctc tgccatataa
    25261 aactttttct ttgtctccta aaatcacatt tgttttttct tcattgatat tttggatgac
    25321 cgaaaccact tctggcaatt cttcatgaat agcttcagca atttccttgg atttaaagaa
    25381 tttttcttta cgagtaacta aaacgaccat catttcatgg gaataatgac cacgacggat
    25441 aatgatgtga cgaagctgtc cttcattttt ttcttcatta tatcctcgta cttggaaccg
    25501 ttgcaagata tctctgacta ctttgattgc ttgatcgatg acaggatctt ggatataaaa
    25561 atcttcgata gggactagat catggctatt ctttttgtaa aaaccggtgg ctaactgtcc
    25621 atcgatacgg cgaacaggga tctgtgcttt attacggtag ccgaatggtt ctgccattcc
    25681 gaccgttgga agtacaggaa cttctggcat cttagcaatc ttcgccatta catgctctac
    25741 ttgtttttgt ttgaagacaa gctgttgttc atacttcaaa tgggctaaag gtgcgatacc
    25801 tatgcgaagt aatgcattgt tttctacagg ctgacgatca ggtgattgtg taataaatgt
    25861 ttcgactttt ccatatccaa acttattgcc tgcttttact actagtattt ctacttcttc
    25921 tccagggagt gcattttcga taaataatgg ataaccatcg atttttgcta cccccattcc
    25981 ttcataactc aagtcgacga tttcgactgt atatcgttcg tttttcttta ctatttgctg
    26041 ttctgtcata ttttgctcct ttaagcctat ttttcaatcc tactgaactc tactataaat
    26101 cagctaatta cacaactaaa accggcactg accttactac gttcagcacc ggcttttgat
    26161 taattctttt gattaggtgt ttcgtctgct atttttccgt caccgtcgat atcttcatcc
    26221 gttaaacgtt cgacttcttt tacgaattct ttgctgacaa cctctaattc ttcttcatct
    26281 gtacctgtga ttgcttcatc aggaatctga tcggtattgg caaagaactc aatgtgttga
    26341 tgaagatttt ggaaatgcat cggtgcttcc ccgccgtatt caccatctaa gttgatcatt
    26401 actggtcgat tgatatcatc ttctgtttca acgaacaaac tgcttgtttt ggtatagatt
    26461 attcttggat catcgacatg tcgaccgcca ttcagcatga gtgctgcaat atgcaagatc
    26521 tcaaagatat ttgctgtctt cacaatgatt aaagaaaact tcccatcatc caatttggca
    26581 tctggcgcga tctgttcgaa tcctccaaca gagttcgtca gccctaggaa aaacatcgaa
    26641 gcatttccga tatattcccc ttcatcatac ttcaagcgca ttttgattgg ctttactcga
    26701 gggagcatct ctgctccttt cgcaagataa gcaagataac caaaaatact ctttagttct
    26761 gatggaactt cataagtcaa ttctgtcaaa tagccacctg cagcgatatt gatgaagtaa
    26821 ctgttatcag aaaggccgat gtccattttt acggtttggt gtttttttat cacttctgct
    26881 gctgctttaa tattatctct aggaattttt aatgctcgag catagtcgtt cgttgtacca
    26941 gctgggatga tggccatttt aggacggtgt tcaagcggag cgattccatt aaccacttcg
    27001 ttgattgtgc catcgccacc agctgccact actagatcaa aacctagttc tgcgactcta
    27061 catgcttcat tcttggctga atctggttct ggtgtagttg cataggcgct ggcttcaaat
    27121 cctgcttctt ctaatgcctg taggatatcc gccaaatttt ttttcaataa ttcttttccc
    27181 gaggtggggt tataaattac gcgtgctttt ttcattttca gcctctatcg tttcgctaat
    27241 tcatctttaa gtagcttatt cacgacacca ggatttgctt gtcctttagt tgctttcatg
    27301 atttgaccga ctaggaagcc aaccgcacga tcttttccat ttttgaaatc atcgacagat
    27361 tgttggttgt tatccaaaat ttcattaatg attggcagta gtttgttcgg atcagataat
    27421 tgcacccaac catttttttc aactacttct tgggcttctc cgccatttgt gatcaattca
    27481 cggaagactt ttttggcgat ttttgagctg atcgttccat cggcgatcaa acgaatcatt
    27541 cctgctaagt tctcaggtgt caattttgtc tcagctaatt ccagtttctc actgttcaag
    27601 taagcagaaa cttcacccat caaccagtta gatgcttgct tggcatctgc accattttcc
    27661 aaaacagctt caaaaaaatc agacatttct tttgtaagtg tcaataccat tgcgtcatac
    27721 tctggaagtc caaactcacg gatatagcgt tcgcgacgag aagctggcat ctcaggcaaa
    27781 ctagctttta ctttttgaat ccattcgtca tcgatcacga atttcgggat atcaggctct
    27841 gggaagtaac gataatcact agacccttct ttgacacgca tcaaaactgt tttattcgtt
    27901 gtttcgtcaa aacggcgtgt ttcttgttgg atttctcctc ctgaaagaag aacttttgct
    27961 tgacgttttt cttcaaaagc taaacctttt ttcacgaaag ataaagagtt caagtttttc
    28021 aattctgctt tcgtaccgaa ctcttcttgt ccataagggc gaagagaaat attggcatca
    28081 cagcgcatag agccttcttc cattttcaca tcgctgactt ctgtaaattg gataattgat
    28141 cgcaatgctt ctagataagc gtatgcttct tctggtgaac gcatatcagc ttcagaaacg
    28201 atttcgatca aaggcgtgcc ttgtcggttc aaatcaacgt aagaataacc gccaatacca
    28261 tgcatgtttt ttccagcatc ttcttctaaa tggacacgtt cgatccggat ttttttcttt
    28321 ttaccttcta cttcgatttc gatccatccg tcatgaccga ttggttgatc aaattgggaa
    28381 atttgatatg cttttggatt atctgggtaa aagtaatttt tccggtcaaa atgagtatct
    28441 ttagagattt cacagtttaa tgccaaagct gctttcatcc cgaattccag cgcagcttta
    28501 ttcattactg gcagcacgcc tggataaccc cagtcgatca cgtttgtatt gctgtttggt
    28561 tctgcaccaa aatgagctgg cgctggagag aaaatttttg agttggtttt taactcgacg
    28621 tggacttcta gtccaatgac tgtttcaaaa ttcattcgtc tttccctcct aaaatagctg
    28681 gttgttgttt atggaattct gttgcttgtt caaacgcata tgccgcttga tacatcgttt
    28741 tttcatcaaa ataattaccg atgatctgca atccaactgg taatccttct gataaacctg
    28801 ccggtacaga catacctgga agtccagcca agttgacagg gatcgttaag atgtcactca
    28861 tgtacattgt gattggatcg ttaatgttct caccgattcc gaatgcaact gttggtgaag
    28921 atggcccgat gatcaagtca taatctgcaa agactttttc aaaatctcgt ttgatcaatg
    28981 tacggacttg tcctgctttt ttgaagtaag cgtcataata acccgcgctt aaggagaatg
    29041 tacctaacat gatacggcgt tttacttctt caccgaatcc ttctgaacgt gagttgacat
    29101 atacatcatc taatgatttc acattttcag agcgataacc gtagcggata ccatcgaatc
    29161 gttgcaaatt ggaacttgct tctgatgagg caatgatata ataaacagcc acaccgtatt
    29221 ttgaatgagg caagctgact tcttccacaa cagcacctaa tgaacggaat gtttctgctg
    29281 ctttcaatac agcttctttt acgccagggg ccacaccttc gcctaagtat tctttaggta
    29341 aagcaatctt cattcctttg atgtcgcctg tcagtccctc tgcaaaatca ggaactgaaa
    29401 cgcctgaaga agtaccgtct ttttcgtcat aaccgctgat agctgtcaat gctaatgcat
    29461 tatctttcac cgtacgggtc attggtccga tttggtcaag agatgaagca aaggcaatca
    29521 aaccaaaacg tgagacacgg ccataagtag gtttcatccc tacgatgccg ttaaaagctg
    29581 ctggttggcg gatacttccc ccagtatcac tacctaaaga aactggaatt tgtccagctg
    29641 caactgctgc tgcagaacca ccagaagagc caccaggtac ttttgttggg tcccaagcat
    29701 tttttgtttt cttgaaataa gaagtttctg tacttccacc catagcaaat tcatccatgt
    29761 ttagcttacc aacagggatc atatctgctt gatagatttt atccatgacc gttgcatcat
    29821 aaattggttc aaaattgtaa aggatcttag atgcagcggt tgtcaagata tcttttgtta
    29881 cgatattatc cttgatacca attggaatac cagcaaggac gttgctttca tcaatccctt
    29941 tttcatctaa agcttttgcc tgttccaatg ctttttcttc gttcaatgta atgaatgcct
    30001 cgacctcagg ctctgtttct ttgattcgtt caaatgttgt ttttgtcaaa tcttgtacag
    30061 tgatttcttt cgatacaagc atatgatgca gttcttctag agaacgattg tgtaattctg
    30121 tcattatgcg ccagcctccc cattatcgat gattgcgggt actttgataa agccgtcttc
    30181 tgtttccggc acatttttca ataattcatc gcggttccat ccagcttgcg cccgatcttg
    30241 tcgcatcacg tttactgttt caaccacatt cgacgtaaat ggaacacctt ccgtatctac
    30301 ttcttccagc atttcaacca tatcgatgat tttacctaat tggtcggtaa aacttgcaag
    30361 ttcttcatct gcgaacgcaa gttttgctaa tttggcaacg tgtttcactt gttcttcact
    30421 aatcgccata ctaccccttc tttctaaacg agttaaaaac tttgctctct attttaacac
    30481 aagagtgccg tgtctgaaat tgtttattga aatttgtctg aaaaaactca tttttcattc
    30541 ttctactgag cgacttttag ccaatgcatc tgccatttga tcttctgtcc aaacttcgat
    30601 tcctaattct tgtgctttgg tcagcttgct gccagcatct tctcctgcta ccacgatatc
    30661 cgtttttttc gatacgctac cagtcacttt cccgccaaga ttttcgatcg tctcttttgc
    30721 ttcttcacga gtaaatcgtg ttagtttacc agtaaggact actgtttttt ctttaaaagg
    30781 agactcgact tcttgcaatt gggcatttcg aagccctttg tattcgaaat tgacgcctgc
    30841 ttgttttaat tcattcatca attcatggac ttcttcgtta ctgaaataag tgactacact
    30901 gtcggcaatg atatcaccga tcgtatcaag cgcaataatc gactcatagt cacttttact
    30961 caatgtttct agatcaccga aatgttctgc taaaattttt gctgctttcg ctccgacatg
    31021 tcggattcct aacccgaaaa tcaaccgctc gaccgaatta tctttgctgt tgtcgattgc
    31081 tgtcaaaata ttatttgctg atttttcttt tattttgtcc aacgtcaata actcttcttc
    31141 tgtcaatttg taaagatcag caacatctgc tactagtttt ttatcataca tctgttctag
    31201 tacgcgcggc cccaacccat cgatattcat cgcatttctt gaaacaaagt ggttcaaacc
    31261 ttctttcatt tgagcagggc atttaggatt gatgcaccgt aaggctactt cttcatccaa
    31321 atggacaagt tcacttccac aaactggaca atgtgtaggc aattggtatt cttcgctgtc
    31381 tttaggtcgt ttatctaaca cgacttgcga aacttctggg atgatgtctc ccgctttata
    31441 aatcagaact gtatctttca agcggatatc cttcaactta atataatcac cgttatgcag
    31501 gctggcacgg cttactgtcg ttcctgctac tcggactggt gtcatgatag ctgttggcgt
    31561 tactacccct gttcgaccga ctgtccattc gatgttttcg attagcgttt caacttcttc
    31621 tggcggaaac ttgtaagctg ctgcccatct cggtgctttg atagtaaaac caagttgatc
    31681 ttgtagcgag aattcattca ctttgatgac gattccatcg atttcatagg gcaaatccac
    31741 acgtttgtca tgatattctt caatgtatga ccacacttca tcaatcgaat gacatagacg
    31801 tttttcccga ttcgtgtgga atccaatttt ttctagctct tctagcgcat catactgggt
    31861 ttttgcttgc ataggaccga aatcagctac tgtgtataaa aaagtgtcca gatttctttt
    31921 cgctgtgatt ttagaatcta actgacgcaa agaaccagct gcggcgttcc gaggattcgc
    31981 aaagatatct tttccctctg cttctctgtc ttggttcaat tggacaaatg accgttttgg
    32041 catgaaacat tcgcctctta cttcgatgtt cattggttct tttaatttga ttgggaccga
    32101 ccggacagtt tttaggtttt cagtgatgtt ttctcctaca gttccatccc cgcgagtcgc
    32161 acctctcacg aaatttccat cttcataacg caaagaaacg gaaagtccat ctatttttag
    32221 ttcacagcaa tagtcaacta cacgtcccac tgctttttgt acacgttgat caaaagctat
    32281 cagctcttct ttgctgaaaa cgtcgttcaa actgtaaagc ggaatatcat gagtgacttt
    32341 ttcaaaacct tctaatactt ttccgcctac tcgttgagtg ggagaatcag acgtgatcaa
    32401 atcaggatat tgttcctcga ttgctactaa ttctgcgtat tctttatcat aaacatagtc
    32461 ttccactgtt ggtttatctt ctacgtagta ttcgcgagac cattggttca gtcttgtacg
    32521 aagttcttct gctcgtttag cagcttcgtc aatcgtcatt tcatgttcca tcatttaccc
    32581 tccactacta agctttttca attggcgcaa aggcagccaa aagacgtttt actccttgac
    32641 ttgggaaagc cacatctagt tctagatcct tcgacgtccc gcttactcgg acgactgttc
    32701 ctagacccca ttttttgtgt ttgactttat ctccaggttt ccagctgtta ttttcccctc
    32761 cagtagcact tttagaggga acaaccgctg tctctggctg tttatagcta tatgctgttt
    32821 ttttagcgaa tacaggcgtt tttttaggcg taggacgcat gccttcgatt tccagtaact
    32881 cctgatcgat ttcttctaca aaacgagaag gtcggttata ttgcgtccgt ccatataatg
    32941 ttctggagaa agcatttgtt aaatacaacg cttcttctgc tcgcgtgatc cccacatatg
    33001 cgagacggcg ttcttcctcc agttcgcttt cttccatcaa tgctcgagat aaaggaaaaa
    33061 ccccctcttc tagaccaatc aggaatacaa ctggaaattc taatcctttg gctgcatgca
    33121 aagtcatcaa tgtcacttgg gaagcatctt cttctaaatt gtcaatgtcg gaaacaagtg
    33181 ccagatcatt taagaagacc gttagttttt cttctggtgc atcagcgtct tcttcatttt
    33241 gctgttcgaa ttgtttgtcg aattcttgtg tgaccgttaa aaattcctcc aagttttcga
    33301 tgcgtgcttg tgcttctaat gtattttgga tttttaagtc ttctagatat ccgctacgat
    33361 ccaaaacctc tttcgtcaat tctgtcactg tcaagtatgg gatcatctga gtgacttctt
    33421 ggatcatctc tccaaatgca cctaattgct gtcctgcttt tccaccgata tttgccagat
    33481 ccacattttg cgctgcttca agcagtggcc attcatgaag agaagcaaaa ctgcgtagtt
    33541 tttcgataga tcctggtccg atgcctcgtt tgggcgaatt aacgatacgc tcaaaactga
    33601 ttgaatcttg aggatttacc agcacattca aatacgctaa aatatctttg atttctttac
    33661 gatcgtagaa cttgtgccct ccaaccattt tataaggaat atttgctttc aacagagttt
    33721 cttccattac acgagattgt gcgttcgtcc ggtacaagat ggcaaagtcg ccgtaattcc
    33781 gatgattgct tcgaatctct tcttgcatcc ggtctacgat aaaacgggtt tcgtctcgtt
    33841 cattatctgc acggtagtaa gtgatcttgt ttccttcttt gttttctgtc cacaagtttt
    33901 ttggttttcg attgctattg ttttcaatga cttggtttgc tgcgctaagg atgtttttcg
    33961 tggagcgata gttttgttct agcaaaatta ctgcagcatc tggataatct ttttcaaaat
    34021 ccaagatgtt ctgcatatct gcaccgcgcc agccgtaaat actttgatct gcatctccca
    34081 cgacgcacag gttcctaaat ctacctgcca ataaattgac tagcgtatac tgtgcgtgat
    34141 ttgtatcttg gtactcatct acatgaatat aatggaattt gttttgataa tacgtgagtg
    34201 aatcaggatg ttcttcaaat aatcgaatcg tattcatgat caggtcgtca aagtccatac
    34261 attgattata ttgaagctct ttttggtaag cagcataaca ttttgccgca atctcttcgt
    34321 aataactgcc ttgcattttt gcaaaatcct gaggggtctg caggctgttt tttgcttgag
    34381 agattgttcc taatatactt cgaggatcat attttttcgg atcaatgttc agttctttta
    34441 gtatccgttt catcaaggtc agctgttcag aagaatcgat gattgtaaag ttccggttat
    34501 acccgataaa atcaacatct cttcttaaaa tacgcacaca catcgagtga aaagtcgata
    34561 cccagacatc ttcgcctcca gatgcgagaa tcgcattgac acgttctttc atttcttttg
    34621 cagccttatt ggtaaaagta atcgctagga tgttccaagg attgacttct ttttcctcaa
    34681 tcaaataggc aatgcggtgt gtcagcactc tcgtctttcc acttcctgca cctgccatta
    34741 gcagtaatgg tccgtcagtg tgaagaacag cttctttctg acgggggttc atcccgttta
    34801 ataattcagc tttggatgac attaacaaca tcctttcttt tatcaatctt gttaattata
    34861 gcattttctt ggctttcatt aaactagcta gttcaaaatc atttgtttct ttttatggat
    34921 ttttccttgt caattcgttg atttcgcgct aaattaaaag aaagcgaggc gtttgaatgg
    34981 aaaaaaaagg aaaaatcaag aaattagata agactcagat atattttatt ctaaaaggaa
    35041 tatttgttgg actgctgaca ggaatcgtcg ttagcctgtt tcgattatct atcgaaaagc
    35101 taagcgattt agttcgaagt atctatgaaa tgagtcggga acagccgatt tacttaattg
    35161 gcctagctat ttgctgcatc cttgcagcat ttttcgtggg ttatctggtc aaaaatgaac
    35221 cggatatcaa gggaagcggg attccacaag tagtgggaca gcttcgcggg gaattatcaa
    35281 tgaactggtt ctctgtttta tggaaaaagt tcattggcgg tgtactttca gttggtgcgg
    35341 gcctttttct aggtagagaa ggaccttcta tacagcttgg cgcatccatt ggacaaggag
    35401 ctggacaatt gtttcggagt ccatcttcag aagaaaagat tttaatttct agcggtgcaa
    35461 gtagcggact tgcagcagca ttcaatgctc ctatcgcagg attactcttt gttttagaag
    35521 aaatccatca ctccttttct ccacttgttt ggctgacatc atttgcttca gcaatcactg
    35581 ctaacttcgt ctctctttac tttttcggct tgagacctgt tttatatcta ggtgaccttc
    35641 cttctttgcc gttgaaatat tatggttcct tagtgatctt aggagtactt ctaggcatct
    35701 taggttttat ttatcagaat gtcttgctgg ctttgccaaa attttacaac cgattgcctt
    35761 taccagctta ttttcacggg ttcgttcctt tcttgctgat tttgccaatt ggttatttat
    35821 ggcctagtac tctaggcgga ggcaatcaaa tcgttttagc ttttggacat gcttccttgc
    35881 caatcattat gattatcggt ttgtttgttc ttcgattcgt tttttccatg atttcttacg
    35941 gtgggggtct tccagggggg attttccttc cgatattgac attgggggct ttaatcggta
    36001 gcttttatgg aaacattatg gttgatttcg ggatggatcc aatctacatc aaggattttg
    36061 tcgttattgc catggctggt tatttcacag cgattggaaa agcaccgctg acagcaatta
    36121 ttttagtgac agaaatggtt ggctctctta atcatctgat gcctttagga ttggctgctc
    36181 ttgttgctta tatcgtcaat gaccttctcg gcggtaatcc aatttacgaa tcattattgg
    36241 aacgtctact tggaaatact cagaccgcct cactaaaagg tgcaaggaca acttttgatt
    36301 ttccagtaac ggcagagagt tctttagacg gattaatggt acgagacttc aattggccta
    36361 aaaatatgct gctaatctct atacgcagag gaaatcagga aatcttgact cacggtgata
    36421 cgatcatgaa tgtaggagat attttggtta tcttgactga tgaagcaaac ttgccaagaa
    36481 tcaagcagga aatcgaggct aaatcttcta tcacaaattt gaagcaatca ttttaacgaa
    36541 catggaaaaa ctattttaca acaactattc atctacacta cttgattcta tatgattcta
    36601 taaaaaa
//
LOCUS       DACEMO010000005        36426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEMO010000005 DACEMO010000000
VERSION     DACEMO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.005"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IW728_000188"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IW728_000188"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1023575.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IW728_000189"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IW728_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1023576.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IW728_000190"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IW728_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1023577.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IW728_000191"
     CDS             complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IW728_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1023578.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IW728_000192"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IW728_000192"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1023579.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IW728_000193"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IW728_000193"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1023580.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="IW728_000194"
     CDS             complement(8054..8524)
                     /locus_tag="IW728_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1023581.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="IW728_000195"
     CDS             complement(8570..8995)
                     /locus_tag="IW728_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1023582.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="IW728_000196"
     CDS             complement(9062..9331)
                     /locus_tag="IW728_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1023583.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="IW728_000197"
     CDS             complement(9342..10937)
                     /locus_tag="IW728_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1023584.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IW728_000198"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IW728_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1023585.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="IW728_000199"
     CDS             complement(14490..15050)
                     /locus_tag="IW728_000199"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1023586.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="IW728_000200"
     CDS             15403..16377
                     /locus_tag="IW728_000200"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1023587.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="IW728_000201"
     CDS             16547..17947
                     /locus_tag="IW728_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1023588.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="IW728_000202"
     CDS             complement(18025..18747)
                     /locus_tag="IW728_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1023589.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="IW728_000203"
     CDS             complement(18826..19332)
                     /locus_tag="IW728_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1023590.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="IW728_000204"
     CDS             19455..21254
                     /locus_tag="IW728_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1023591.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="IW728_000205"
     CDS             complement(21322..22896)
                     /locus_tag="IW728_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023592.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IW728_000206"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IW728_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1023593.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IW728_000207"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IW728_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1023594.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="IW728_000208"
     CDS             complement(24673..25311)
                     /locus_tag="IW728_000208"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1023595.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="IW728_000209"
     CDS             complement(25312..25962)
                     /locus_tag="IW728_000209"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1023596.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IW728_000210"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IW728_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1023597.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IW728_000211"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IW728_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1023598.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="IW728_000212"
     CDS             complement(29105..29845)
                     /locus_tag="IW728_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1023599.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IW728_000213"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IW728_000213"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1023600.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="IW728_000214"
     CDS             complement(31216..32154)
                     /locus_tag="IW728_000214"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1023601.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="IW728_000215"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="IW728_000215"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1023602.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IW728_000216"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IW728_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1023603.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="IW728_000217"
     CDS             complement(35314..35619)
                     /locus_tag="IW728_000217"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1023604.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IW728_000218"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IW728_000218"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1023605.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9846 a         7971 c         6672 g        11937 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatgga aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggcaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cccagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ctaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgatatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgttt
//
LOCUS       DACEMO010000006        35540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEMO010000006 DACEMO010000000
VERSION     DACEMO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.006"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1031)
                     /locus_tag="IW728_000219"
     CDS             complement(<1..1031)
                     /locus_tag="IW728_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1023606.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IW728_000220"
     CDS             complement(1113..1802)
                     /locus_tag="IW728_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1023607.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IW728_000221"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IW728_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1023608.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IW728_000222"
     CDS             complement(2329..2832)
                     /locus_tag="IW728_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1023609.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IW728_000223"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IW728_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1023610.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IW728_000224"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IW728_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1023611.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IW728_000225"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IW728_000225"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1023612.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IW728_000226"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IW728_000226"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1023613.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IW728_000227"
     CDS             complement(6444..7463)
                     /locus_tag="IW728_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1023614.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IW728_000228"
     CDS             complement(7636..8523)
                     /locus_tag="IW728_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1023615.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IW728_000229"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IW728_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1023616.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IW728_000230"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IW728_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1023617.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IW728_000231"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IW728_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1023618.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IW728_000232"
     CDS             9754..10398
                     /locus_tag="IW728_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1023619.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IW728_000233"
     CDS             complement(10454..10957)
                     /locus_tag="IW728_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1023620.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IW728_000234"
     CDS             11554..12519
                     /locus_tag="IW728_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1023621.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IW728_000235"
     CDS             complement(12618..13790)
                     /locus_tag="IW728_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1023622.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IW728_000236"
     CDS             complement(13777..14922)
                     /locus_tag="IW728_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1023623.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IW728_000237"
     CDS             complement(14936..15868)
                     /locus_tag="IW728_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1023624.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IW728_000238"
     CDS             15985..17064
                     /locus_tag="IW728_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1023625.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="IW728_000239"
     CDS             17061..17690
                     /locus_tag="IW728_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1023626.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="IW728_000240"
     CDS             complement(17800..18027)
                     /locus_tag="IW728_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1023627.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="IW728_000241"
     CDS             complement(18130..20718)
                     /locus_tag="IW728_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1023628.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="IW728_000242"
     CDS             complement(20738..21397)
                     /locus_tag="IW728_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1023629.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IW728_000243"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IW728_000243"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1023630.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="IW728_000244"
     CDS             complement(22170..23036)
                     /locus_tag="IW728_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1023631.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="IW728_000245"
     CDS             23145..23786
                     /locus_tag="IW728_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1023632.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IW728_000246"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IW728_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1023633.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="IW728_000247"
     CDS             complement(25227..26126)
                     /locus_tag="IW728_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1023634.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="IW728_000248"
     CDS             complement(26128..26925)
                     /locus_tag="IW728_000248"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1023635.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="IW728_000249"
     CDS             complement(26903..27607)
                     /locus_tag="IW728_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1023636.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="IW728_000250"
     CDS             27738..28313
                     /locus_tag="IW728_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1023637.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="IW728_000251"
     CDS             28431..29108
                     /locus_tag="IW728_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1023638.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(29198..30493)
                     /locus_tag="IW728_000252"
     CDS             complement(29198..30493)
                     /locus_tag="IW728_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1023639.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            complement(30698..32506)
                     /gene="pepF"
                     /locus_tag="IW728_000253"
     CDS             complement(30698..32506)
                     /gene="pepF"
                     /locus_tag="IW728_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1023640.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(32682..33680)
                     /locus_tag="IW728_000254"
     CDS             complement(32682..33680)
                     /locus_tag="IW728_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1023641.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(33906..34559)
                     /locus_tag="IW728_000255"
     CDS             complement(33906..34559)
                     /locus_tag="IW728_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1023642.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(34891..35289)
                     /gene="spxA"
                     /locus_tag="IW728_000256"
     CDS             complement(34891..35289)
                     /gene="spxA"
                     /locus_tag="IW728_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1023643.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        10485 a         7471 c         6285 g        11299 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaaaa aaggctcttt gtcaaatgat gttggtaaag agaaatctta gaataatttg
    29161 gacagaacag aaagaagaaa acctctttct gttctgttta tttggttaaa aatgttttgc
    29221 catgacgaat cataattcgt agtttgaaat tgtaaaagtt gcgaaagccg tacgcattgc
    29281 gttttaatac tttgatgtgg ttgtttaagc attccaatgg tccgtttgaa tagctgtagt
    29341 gaagcgcatt ttggatctgt ggcagaaact ttttaaacgt tcgtaaagtg gttgtatagg
    29401 tttctggcag accgctaatg tctctagtta aacaatcctc aaatagtgac tgatctcttt
    29461 tctttaccgc ataaagaatg tcttggtata actgatagcc ttggcgtaag ttgggacaag
    29521 cagctaacag tcgatcggca atctcacttt cagtcaaata atctttaaaa ctggcacgcc
    29581 aaacccgttt ttcatagttt aactttgctt cttctttttg taggagtttc caatagcgct
    29641 tcaatttttt ccctaaccct cgttgagagt gggaatttcc tttaagtcgt accgtggttt
    29701 ctttcactcg atgattcaag aaagttctgc caatatgttg gacgatgtgg aaacgatcga
    29761 tgatcaactg agcattcggg aaaagctttt tcaccaacga aacataaggc gcatacatat
    29821 caataacaat caattgaacg gcttcacgag tcgctaatga aaaccgagaa aaatagcgtt
    29881 ctaaataagg caagcgtcga ttttcgacaa tatccaataa tgcatggctt tgtccgtcca
    29941 tcatgataaa actcattgca ctggcaacac ttcgaaccga tttgaattca tcaaagcata
    30001 agacctttgg caaagtctgt ttgaaaggat tcaatgggcg gtagaatcgc ttcatgacac
    30061 gatagaccga agaagtggaa atatttttct gacgagcgat ttccgtcatg gaagtattct
    30121 cttttaagcg agcaatgaca gaccaacgaa ctttttgact aatgaaacag tgtttttcag
    30181 caacagaggt atccgctacg aatgttcttt ggcaagactt acatttaaat cgttgtttct
    30241 tcagctccag ataagtgcga tactcagaca catcattcag taacaaccga acggttgtcg
    30301 tcccccaacg aatgatccta gaggggacga cacaaagaca atgggaacac tcttctggct
    30361 tgtacagaag agttcctttg taaatcaagc aaatttgccc tttgtatttt tctttagtta
    30421 aacaattttc ctcgaaatag atatttaggt ctagaatatt tagggtatct ttgataagct
    30481 gagtatgaga catcttgaat cctcctatga attggtagcc aaattaattc taaaggattt
    30541 cagggtgtct tttctatatt tttttataca aaaaagatgt tggtgaagtt caccaacatc
    30601 aaaaagtata gagccttaaa aaaatacgtc gactccctta caagagagcc gacgtatttt
    30661 tttcgtttct attcgagaaa atcaaaaata gaaagcctta taaacgatcg atcaattctt
    30721 ctaattcatt tagacgttgt tcaaacatcg acattgcatc ttcaatatat gcagcctgtg
    30781 tcatatcaac tcctgctttt ttcattactt ccacaggata atcgctgtta cctgctttta
    30841 ggtaagccaa gtagttctct agtgcctctg gttcctggtt tagtattttc ttcgccagtg
    30901 ctgaagcagc agaaaagcca gtcgagtatt ggaaaacata gtaattataa taaaaatgcg
    30961 gtatccgtga ccattcgaat ttaatttctg ggtcttcttc gactgctgga ccataatatt
    31021 ttgcattcaa tttaccataa ctatcactta ggtattcact agtcaatggc acaccttttt
    31081 catcttccgt atgcatgaaa tgctcgaatt cagcgaattg tgtctggcgg aaaactgttc
    31141 ctttaaaccc atccaagtaa tggttgagta cataagcccg tacacgaggg tctttttctg
    31201 tttccaataa atactccgtt aggatatttt cattcgttgt cgaagcaatt tctgctaaaa
    31261 agatggagta gtcgccgtac acataaggct ggttcgaacg agtgaaataa ctatgaacac
    31321 tatgtcccat ttcgtggaca agcgtaaata gctgatccag cgtatcatgc caattcaata
    31381 aaatatatgg atttgtgtca tagcttcccg aagaataagc accgcttcgt ttccctttat
    31441 tttcgacaac atcgatccaa cgttcagaga atgctttttc tacgatggcc atgtattctt
    31501 cacccattgg tttcagtgct tctaaagctt tttcttttgc ttcttcgtac gtaaaggtaa
    31561 ttggagcttc ccctaagacc ggtgtataaa gatcatacat gtgcagtttt tccacttcta
    31621 ataaacgttt ccgtaattcc atgtatcgat gtaacaaagg caaatgcttg tttaccacgt
    31681 ctaccaaagt atcgtataca ctttcaggaa tatgattatt gctcaaagac gcttctctgg
    31741 cagagctgta attacggact ttcgctttaa aattatgtcc ttttatatgt gtgcctaaag
    31801 tagaagcaaa tgtatttcta aattgttcgt aaacactgta caacccctta aatgctgctt
    31861 cgcgcaccct tcgatccgtg ctttctagca actgaccata cacgccatga gataattgga
    31921 ctatttcacc attttctcct tcaatcgttg gaaaaactag atctgcatta ttcaaaacag
    31981 caaatgtatc acttgaagca tcaaagattt cacctgctcc agcaagaaga gattcctgct
    32041 cagcagataa gacatgggct cgattatcta ctatttgctg gatataatgg cgatagactt
    32101 ccaattttgg ttcttctttg aaatattgcc aaatctggtc atctgacaat tgcaatattt
    32161 ctggttcaaa ccaggaaacg gcttcactaa ctttcgaaaa cagactgctt gctcttgcat
    32221 aaagcgcttg gtaatctgta tttccagtgt cttgatcgtt tttaaggtgc gcatatacat
    32281 aaatgacttc agtttggcga taaaccctca atacgaattc aatcgcattt aaaaattgag
    32341 aagcgccttg atcaagtgtt cctttgtgtt tttcagattg ttttaactct tctgataatt
    32401 ccaaatattt ctcatcaaac tcttggtcgc ttgaaaagat cttggtcaag tcccaagtta
    32461 aattttcagg caattcttct cgttttggca actgctttac ttccatgaaa tccactcctt
    32521 ttcttccctt agctctatct atcaaaatag atcatagtct aacatccaca tgtcacagca
    32581 tctaactttt ctactcattc atgtgtctgc ttccatcaat tttagcataa ctaaatcttg
    32641 ttttacatag aaaaccgatt cctagcctat ttttatactt actatttttt caaagaagac
    32701 aagtgtatgc attcgttaaa aaactgctga tagaccatct cttcatcaat cataggaaaa
    32761 agccattccc ttttatgttc ttttatttgg caaagccatg agttgaaatc aggaactttc
    32821 tggttttcca aggcttcttc gtacaacatt cgataaagaa aaacctgttc ttgtaaataa
    32881 aagaaaaacc gacttttttg atagatccac gatgatagat gcaaaagatg tttatgacgc
    32941 aagtagcatt gttcttgtat ggatactgta ttcttttgct tgtgaataag ttggcgtgtc
    33001 agccatttct tcccatcttc actcactttt tctatttttt tcatttggtt cattttaatt
    33061 tcgttactag tgaatatttc tttaaggtcc ctactagaaa aagtccagca gctactgcaa
    33121 aaggaaatat gccctgatac agtctctgta atatgatgat acaaataaag cttttgtttc
    33181 ttgagatcag ctttccagca atgcactcct ctttttctat tatagtagca gcaacttttt
    33241 tctatcaatg agaattgcga ttgtccaaga aaagaattac caagtatcca ccatacttgg
    33301 taactgttat tcaaataatt ttgggttctt tcaataaatc tctggtgaga cagtctagaa
    33361 cattggattt ctattgctag attcgccaac aaaatatccg gcctttgccg caacctcggc
    33421 aaataggctt ccaaaaatac tggttctact gactgtccca gccaatccat gaagcattcc
    33481 tttaactgta agtgctcttt tgattctctt tcactaaaga ccgaacagtt atctgatttt
    33541 tgatgggcaa aatgagcaca ttttttcttt ccctttttca aaatcactct ttttcgacag
    33601 ccacgacaat aatatttctt tcctttttct gcatgtttag caaaacagca cttattatat
    33661 tcattcattg caagcagcat ttttctccct ctgaacttat ttacgtctaa gaatttattt
    33721 acacgaaaaa agagggcgtg aaaaagctat tctgcatcaa gtaataagaa cagctaaaca
    33781 aaaatagttc ccaatatttt tcgtttggtt gggcttgtta ccgcagatgc aagcttttga
    33841 acacatttat acgaaaaaaa gactgcgaca caatttttgt cacagtctct tactataatg
    33901 ctgatttata caaaataaaa tttcgttcgc tcgatagcat tacgcgccat cagacattgt
    33961 ccatattcat tcaatgtttc tggtgtacga tcgctctttt cagcaaactc caaaattcga
    34021 gcaatctgat tctctacatc cgcttggttg agattttcca tccagaagtg aacgactagg
    34081 taatagcgct cttcgaataa atacaaatca gtccaagcag actctagttc gacatctgtt
    34141 gcaagttgga tcacttgttc aaaatcttca aaagcaaaag ttgcttgtct ttcctgttct
    34201 tctagggcgc ttccagataa ttcttcctcc ggatcgctgc caataatctg ctgacgaaga
    34261 aagtcactca catcttctga atcaacagta gaatcttctg attgagaaat ttcttcatcc
    34321 ggaagatttt tactgataaa cagttctaat ccgtctcctt ttggcaaaac ttggaaagtg
    34381 acagcttcgc ttcctcggaa ctcctcttcg atatcaactt cttccaaaat actataaaag
    34441 aaattttcga tttctttgtg attgccaagt aaatccaaaa atgaaacgcc ccgttcctct
    34501 aaatcttcat gaccaattag tacacggatc gtattttcat taatatgttc catttccatt
    34561 ttgctacacc tcactttgcc acaatgttta atatagattc attgtagcga aatcagctta
    34621 aatgtaaaga aaaaaaatct cgctgttaag cgaatttaat aaaaccagcg gatttttcaa
    34681 cctttcaagc tatcttattt aactataaca tttttggcag acgaatacta tatagccgac
    34741 ttttcagcta attttccctc tctatttttc gtgcaatcat catcttctac taccttttta
    34801 tacatctaat caagaatact ttttgtaaaa acaaaaaaac ttggaaaaac tatctttgta
    34861 atagtctttt ccaagtttcg tattatcctt ctataatcca gccatcaatt gtgcttgacg
    34921 taattctaat tgtcgtacat cgcgcggtag gaaccgacga atttcatctt cattaaaacc
    34981 aacttgcaga cgtttatcat caatcatgat tggacggcga agcaatccag gattgttttg
    35041 cacaagttcc aataattctt gtaacggcag atcatctaaa tccatgttca gtttttggaa
    35101 tactttggaa cgtgtcgaga tgatttcttc tgttccgtct tctgtcattt gtaaaatcgc
    35161 ttttaattca gaactgttca atggttctga aaaaatatta cgttcaacaa atggaatctg
    35221 atgttcttgc aaccacgcac gagcctttct gcaggaagta caacttgggg aagtatataa
    35281 tgttaacaac gtgcaccact ccttttcgtg ataagaatca cttttttgta acagaagatc
    35341 ataataatct cccatctgtt tctattatac ataatcaact tttaaatagc tacctttttt
    35401 tcataatttt tttaaaaaaa tgaaagttat ctaaggttaa tatacagtaa aaccagcatt
    35461 ctcgcttttt ttatcattta aaaattagag ttttcaaatc taattcaaat tttctaaaca
    35521 tatgaaacag cgtataaaac
//
LOCUS       DACEMO010000007        35438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEMO010000007 DACEMO010000000
VERSION     DACEMO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.007"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1152
                     /locus_tag="IW728_000257"
     CDS             <1..1152
                     /locus_tag="IW728_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023644.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IW728_000258"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IW728_000258"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1023645.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IW728_000259"
     CDS             2728..3003
                     /locus_tag="IW728_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1023646.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IW728_000260"
     CDS             3009..3776
                     /locus_tag="IW728_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1023647.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGNPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IW728_000261"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IW728_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1023648.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IW728_000262"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IW728_000262"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1023649.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IW728_000263"
     CDS             7920..8222
                     /locus_tag="IW728_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1023650.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IW728_000264"
     CDS             8225..9388
                     /locus_tag="IW728_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1023651.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IW728_000265"
     CDS             9414..12842
                     /locus_tag="IW728_000265"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1023652.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IW728_000266"
     CDS             12863..13987
                     /locus_tag="IW728_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023653.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IW728_000267"
     CDS             13977..14414
                     /locus_tag="IW728_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1023654.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IW728_000268"
     CDS             14398..14736
                     /locus_tag="IW728_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1023655.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IW728_000269"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IW728_000269"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1023656.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IW728_000270"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IW728_000270"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1023657.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IW728_000271"
     CDS             15893..16936
                     /locus_tag="IW728_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1023658.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IW728_000272"
     CDS             17095..17823
                     /locus_tag="IW728_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1023659.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IW728_000273"
     CDS             17939..18184
                     /locus_tag="IW728_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1023660.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IW728_000274"
     CDS             18181..18399
                     /locus_tag="IW728_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1023661.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IW728_000275"
     CDS             18535..19074
                     /locus_tag="IW728_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1023662.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IW728_000276"
     CDS             19067..21340
                     /locus_tag="IW728_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1023663.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IW728_000277"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IW728_000277"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1023664.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IW728_000278"
     CDS             complement(22513..22767)
                     /locus_tag="IW728_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1023665.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IW728_000279"
     CDS             complement(23167..23436)
                     /locus_tag="IW728_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023666.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IW728_000280"
     CDS             complement(23906..24361)
                     /locus_tag="IW728_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1023667.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IW728_000281"
     CDS             complement(24499..26427)
                     /locus_tag="IW728_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1023668.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IW728_000282"
     CDS             complement(26624..27457)
                     /locus_tag="IW728_000282"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1023669.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IW728_000283"
     CDS             complement(27649..28353)
                     /locus_tag="IW728_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1023670.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IW728_000284"
     CDS             complement(28376..29002)
                     /locus_tag="IW728_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1023671.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IW728_000285"
     CDS             29328..31703
                     /locus_tag="IW728_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1023672.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IW728_000286"
     CDS             complement(31727..32218)
                     /locus_tag="IW728_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1023673.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IW728_000287"
     CDS             32405..33325
                     /locus_tag="IW728_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1023674.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IW728_000288"
     CDS             33479..33823
                     /locus_tag="IW728_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1023675.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IW728_000289"
     CDS             complement(34013..34513)
                     /locus_tag="IW728_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1023676.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IW728_000290"
     tRNA            35041..35124
                     /locus_tag="IW728_000290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
BASE COUNT        11776 a         6168 c         7296 g        10198 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtaatcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag gcgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaa
//
LOCUS       DACEMO010000008        33155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEMO010000008 DACEMO010000000
VERSION     DACEMO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.008"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..511
                     /locus_tag="IW728_000291"
     CDS             <1..511
                     /locus_tag="IW728_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ1023677.1"
                     /translation="SLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVFQG
                     ANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDAHY
                     KYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQCE
                     LVNVEKINK"
     gene            517..1683
                     /locus_tag="IW728_000292"
     CDS             517..1683
                     /locus_tag="IW728_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1023678.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            1765..2436
                     /locus_tag="IW728_000293"
     CDS             1765..2436
                     /locus_tag="IW728_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1023679.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            2489..4465
                     /locus_tag="IW728_000294"
     CDS             2489..4465
                     /locus_tag="IW728_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1023680.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            4478..5230
                     /locus_tag="IW728_000295"
     CDS             4478..5230
                     /locus_tag="IW728_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1023681.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            5246..6007
                     /locus_tag="IW728_000296"
     CDS             5246..6007
                     /locus_tag="IW728_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023682.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            6020..6280
                     /locus_tag="IW728_000297"
     CDS             6020..6280
                     /locus_tag="IW728_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023683.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            6277..8367
                     /locus_tag="IW728_000298"
     CDS             6277..8367
                     /locus_tag="IW728_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1023684.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(8403..10460)
                     /locus_tag="IW728_000299"
     CDS             complement(8403..10460)
                     /locus_tag="IW728_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1023685.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(10605..10796)
                     /locus_tag="IW728_000300"
     CDS             complement(10605..10796)
                     /locus_tag="IW728_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023686.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            10984..11439
                     /locus_tag="IW728_000301"
     CDS             10984..11439
                     /locus_tag="IW728_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023687.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            11436..11678
                     /locus_tag="IW728_000302"
     CDS             11436..11678
                     /locus_tag="IW728_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023688.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            11696..11998
                     /locus_tag="IW728_000303"
     CDS             11696..11998
                     /locus_tag="IW728_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023689.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            12067..14121
                     /locus_tag="IW728_000304"
     CDS             12067..14121
                     /locus_tag="IW728_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023690.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            14121..16733
                     /locus_tag="IW728_000305"
     CDS             14121..16733
                     /locus_tag="IW728_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1023691.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            16730..19108
                     /locus_tag="IW728_000306"
     CDS             16730..19108
                     /locus_tag="IW728_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023692.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            19169..19711
                     /locus_tag="IW728_000307"
     CDS             19169..19711
                     /locus_tag="IW728_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023693.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            19789..20121
                     /locus_tag="IW728_000308"
     CDS             19789..20121
                     /locus_tag="IW728_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023694.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            20122..20715
                     /locus_tag="IW728_000309"
     CDS             20122..20715
                     /locus_tag="IW728_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023695.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            20737..22431
                     /locus_tag="IW728_000310"
     CDS             20737..22431
                     /locus_tag="IW728_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1023696.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            23119..24996
                     /gene="ltrA"
                     /locus_tag="IW728_000311"
     CDS             23119..24996
                     /gene="ltrA"
                     /locus_tag="IW728_000311"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1023697.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            25127..25504
                     /locus_tag="IW728_000312"
                     /pseudo
     CDS             25127..25504
                     /locus_tag="IW728_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            25504..26703
                     /locus_tag="IW728_000313"
     CDS             25504..26703
                     /locus_tag="IW728_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1023698.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            26719..27363
                     /locus_tag="IW728_000314"
     CDS             26719..27363
                     /locus_tag="IW728_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023699.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            27374..28180
                     /locus_tag="IW728_000315"
     CDS             27374..28180
                     /locus_tag="IW728_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023700.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            28203..28432
                     /locus_tag="IW728_000316"
                     /pseudo
     CDS             28203..28432
                     /locus_tag="IW728_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28591..29526
                     /locus_tag="IW728_000317"
     CDS             28591..29526
                     /locus_tag="IW728_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ1023701.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            29570..29839
                     /locus_tag="IW728_000318"
     CDS             29570..29839
                     /locus_tag="IW728_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023702.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            30115..30390
                     /locus_tag="IW728_000319"
     CDS             30115..30390
                     /locus_tag="IW728_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023703.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            30432..30854
                     /locus_tag="IW728_000320"
     CDS             30432..30854
                     /locus_tag="IW728_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023704.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            30874..32466
                     /locus_tag="IW728_000321"
     CDS             30874..32466
                     /locus_tag="IW728_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023705.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            32487..32822
                     /locus_tag="IW728_000322"
     CDS             32487..32822
                     /locus_tag="IW728_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023706.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            32968..>33155
                     /locus_tag="IW728_000323"
     CDS             32968..>33155
                     /locus_tag="IW728_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023707.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        11949 a         5187 c         6801 g         9218 t
ORIGIN      
        1 cagtttaacg gatatttcta agagacaact gattactaat caaaaacagg cattagaaaa
       61 ggatctcaaa ggtgaaattg ctggaaaact tgttttttct tctacaggat ttattctccc
      121 tgtttttcaa ggagcgaata gcgacacgct tagtttagga gtagcttcta cttattatct
      181 agatactgaa atgggaaatg gaaattatat tcttgctggt tataacgtag atacaccaag
      241 cgttttatta tccgatatga ctgatttatc tgaaggagaa gaaattgatc taatcgatgc
      301 tcattacaag tatcagtatg aagtctccaa aagaggaaaa atttctgatt acagccaggt
      361 agttcaatct aaggaaaaca attccttttt atccttgcca aaagcaggta aaaaaccact
      421 actcacactg ttagcctttc aagcaaataa tatgaaaaag tgcgatgtga tccagtgtga
      481 attagtcaat gtagaaaaaa tcaataaata agggaagtga gtcacattca agaacttgta
      541 gaaaaattga tcaataaaat gagtgccatt tttctgttac tgttggtatc cattctatat
      601 ttaatatgga cggtggggtt aaatcctcct acgatattat ttatttatga taatgctgca
      661 cagttagtga cgtttttcgt aggctgtttg tttcttttga atctgtacaa ggtaaagaga
      721 aacgatctat tcttcattgc agcaggcatt cttttttacc ttttcttttc tttttttaga
      781 agctaccgag gagatacttt ctatggtgat ctgatcgttc cgacaattat tgctattatt
      841 ttgattgtaa aatggacgaa atttactgta attgatcgta gcttgtatct tgtagtattc
      901 tatgtctttt tactcgtaac ggtttatcga gtatttactg agataccagt accagaagga
      961 caaagtattt ggaatccatc gaacaaatta tcagatattt ggatcaatac aaatacgata
     1021 ggtagttctt taatgacatt agcccttctt atcacaggat ttgctagttc atttggaaaa
     1081 tggtacatgc gattgctatc attaccagcg ttgattcttg ctggggcaac gatttgggtt
     1141 tgccaatcga aagcagcatt atatgcgctt gttatcttca ttatcttaga aattttacct
     1201 aagaaggttt ttcgaaatac acacttgtcc ttttggatgt atgcagcagt ttcggttcta
     1261 gctttaccga tttcttattt tgctgcaatg tcagacaaag ttcacttatt tacaggacga
     1321 gaagaaattt ggcgcaaatt ctatgaaaca ttaggcgaaa aaacaggaca aatatttgta
     1381 gggatgaaac ctttcttttt ccatagagga aatcagattc taggtaatca taatacgtat
     1441 cacgcactcg tcaatcacta tggattgatt ggttttgttc tatcgattat tcttttgctt
     1501 agtttcttat ggttgctcgt aagaaaacgt gagctgacaa atggacaact aacattcttg
     1561 tgggcttttt tagcgatcat gtgtcaatcg ttcatggaag aaacattaac gacaatcagt
     1621 tgggtgccaa ttgtattctt aatgttagca atggctacac accaatataa aaccaccaga
     1681 taaatgaaat agaatagtaa taagagaaaa cactttttcg ttgtgaaaaa gtgttttttg
     1741 ttttgtctga aaggaggcag atggatgaaa aaatggttat ttggtttttt aggagtcgct
     1801 ttaatcgtcg tttgcagtgt gtttggctat gttagctacc aaaaacatga aggagaggtg
     1861 tttaagcaaa atatagaaaa aaagatgccc gtagatcaaa tcaatgcaca cgcaaagagt
     1921 tataaggagg atgcaacgaa cgtcaataat gatatgtctt tggggcaaat gttgtccata
     1981 caaaaagaag caatcgaaat gggcgtcaat aaacaagtat ttgcacaaat tcagattcct
     2041 gcacttggtt tagcattgcc aatttttaaa ggggcaaatc aatacacttt gagtcttgga
     2101 gcggctactt atttttatga agatgccgaa atgggtaaag gaaactatgt cctagcaggt
     2161 cataacatgg aaatgccagg tgttttattt tctgatattc aaaagttgtc attaggggag
     2221 gtgatggact tagttagtaa cgatggcgtc tatcgttaca aggtaacgcg taaatttatc
     2281 gtacctgagt actttaaatt aatcgatggc gttcctgaag agaactcttt cttgtcttta
     2341 ccaaagaaag gagagaaacc cttattaacg ttgtttactt gtgtttatac gtcacaagga
     2401 aaagaaagat acgtggtaca gggagagcta caataacaag aatttaatca acgcgagtcg
     2461 ttgaaatatg aaatgaggag agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg
     2521 aatgattgtt ggaatgttag gaatcattgc aggctgcttt caggggtatc gagcttatgc
     2581 agaagatgtc acgcaaaaaa cgccaccaga aagggtcaat atcacggttc ataaattaat
     2641 gtacgatcaa agtacgcaat tgaacgttga tattgacgga atcaaaaatg atggttatac
     2701 gcacgatcaa tatcctacag gtgtaacgaa atacaacaag gcagattatg gtgatgttga
     2761 gtttacgtta ggaaatatta cggatcaagt attaccgact gaagattctg atctaaccaa
     2821 cgcgaaagtg gacgaaatcg ttaaagatgt agaagacaag ggatctaaca gtgagtacgt
     2881 taagaatgca accaataaaa ttacttcagc tgtagatgaa aatggtgaga tcacttttgc
     2941 tgatcaacca gcctatgtca attccaaagg gaatgtatat gtggtttatg aaagtaaatc
     3001 cgctgctgga ttagtcacac aaaaagcaaa accaatggtt gtgattgcgc caatgacaga
     3061 caacacagga tcaggatttt taaaagatat tcatttgtat ccaaagaaca ttgtttctaa
     3121 gttgagtttt gaattaacga aatttggtga cgatggcaca gctcaaagca aacaaacacc
     3181 tttaaaaggg gcaaaatttg agttatacaa gggcgaacct ggaaaaggaa caaaattagg
     3241 agagcttgta tcggatgatc aaggaaagct gactgcaact gatttgacgt taggaaaata
     3301 ctactttgtg gaagttcctt cagaagttgt agttggatca gatcaagaac caacagcaga
     3361 ccaatatttg ttaggcgcag acgctcgcaa cgatgcgcac aataagttaa cctttgaaat
     3421 tacgaatgac ggcgtgacaa gtgatttaaa agcttcctac gtgaactata aagcaccagt
     3481 gatcgataaa acagtgacaa atggaacagg agaagaacat tctttccaaa ttggtgacgc
     3541 agtgaattat caaggtacaa ttcatatccc tacagatatc gcaggtggaa cagatgggat
     3601 tacagtaaat ggtgtaaaaa gtgaaacttc tccttacagt gtatttaaat ggggcgacac
     3661 agcaggtaaa ggactttctt atgttgctga aaaagcagat attaaagtaa caaataatga
     3721 tggttcagtc gtattaaaag aaaatactga ctataaaatc gaaaattctg atagtggttt
     3781 tgtgatcgat tttatcgtaa ataatggaca agtaagtgat acagttgcga atttacatgg
     3841 tcaagatcta aaaatgactt ataacatgtt tgtgaatgat agtgctgaaa tcgcaaatcc
     3901 attaacaaat agcgtggatt tcgtttataa caataatcca tttaatcagg aagaacacca
     3961 cgaaaaaacg aaagctgacg ttgtgactta tggcgcaaaa ttcttaaaag tcgatagcgg
     4021 cctatttgga acaggtatca aagcaacacc gcttgaaggc gcagaatttg cagctaagaa
     4081 tgcagaaggt aagtactacg gtggacttac tgatacagat aaagatggtg tcaaagaagc
     4141 agtttgggta gacgatgtag caaatgcagc aatcctaaaa tcagataaag agggtcattt
     4201 tgaaatcact ggtttaactg aaggagatta ctcactggaa gaaacaaaag ctcctgaaaa
     4261 ctatcaaaaa ttaacaaaag agatctcttt caaagtagac aaagattcat acaaagaaga
     4321 aaaccgaatc acgatcaaaa ataatcaaaa agcttccgta ccaatgactg gtagcaacgg
     4381 tttccaatct tacgtattga tctcttgctt gttgcttggg gcaggcgcaa tttctgcagt
     4441 tgtttacttt aagaagaaag cctagaagga tcagctaatg attatcagac accctaagaa
     4501 aaagcgaatc atgggtaaat ggatcattgc tttctggtta ctttcagctg ttggtgtctt
     4561 acttttgatg cctgctgaag cgagcgtggc gaaataccaa caaaatcagc agattgccgc
     4621 aatcgatcga acaggaacgg cagcagaaac ggattcttct ttggatgttg ctaagattga
     4681 gttaggcgat cctgttggta ttcttacaat tccaagtatt tctttaaaac taccaattta
     4741 tgacggaact tccgacaaaa ttttagagaa tggtgtaggg attacggaag gaactggcga
     4801 tattacagga ggaaacggta aaaatccttt aatcgcaggg cacagtggac tttataaaga
     4861 taatcttttc gatgatttgc cttcagttaa aaagggagaa aagttttata tcaaagttga
     4921 tggtgagcag cacgcttatc aaatcgatcg gattgaggaa gtgcaaaaag acgaactaca
     4981 aagaaacttt gtcacttact tagaaccaaa tcccaatgaa gatcgagtaa cgctaatgac
     5041 atgtacgcct aaggggatca acacacaccg tttcctagtg tatgggaagc gagtaacctt
     5101 tacaaaatca gaattaaaag atgaagaaaa taagaaacaa aaattatctt ggaaatggct
     5161 tttaggaagt actgtttttc tttcagtcat gatcattggt tcgctatttg tttataaaaa
     5221 gaaaaaataa aggagggtaa ccaacatgag aaatagaaaa tggctatttg tttgtgcagc
     5281 cgctcttttt tctttcactg cctacacaca gttgacgttt gcagacagac aagaaagcac
     5341 ggatagcaca gaacaaacaa tccaaacgtc agatagcagc acgattcaaa gcagcgatga
     5401 aaccgtcgat tcgtcaacag ttgattcttc agaagaacct aaaattgctg aagacgatca
     5461 aatagtcaaa cagaccattc acgttcaaaa aattatttct tccgaacaat tagacaacca
     5521 ggggcaaaag cttgaaaaac aagaaggagt aaatggcgcg aagttttctg tttatgatgt
     5581 atcggacatt ttacaaaaga tggatgttaa ggatctcact actgaccaaa ttgaatccca
     5641 attaaaagat cgagtgaaaa aactatccag tgatcaactg aagctaattt ctaatggaga
     5701 aacaaagaca attgatcaac aaacaggtgt ctttgaattc tcggtagaag tacaggcaaa
     5761 tcagaaacaa gcttattata ttgtcaatga atcaagtcct gaaaacattt ctaatagcga
     5821 ggatattttg ctattaacac ctgtttctga taaaaatggt gaattcttaa aggatgtgtg
     5881 gatttatcct aaatcagaag cttcgcaacc taaagaagaa gtaaaaaaaa tcgtatctac
     5941 tggcgtaaag aaaaacttct ttgagaattg ttgggatttt gttactcact tgttttctag
     6001 agattaagga agaaataaaa tgaaactgat caaattatta ttattcaatt tttcttgttt
     6061 aaccgcagtc tttttgtact gcggtttatt attttctaaa ctatctattt tgccaagcta
     6121 ccaactatac ctaatgagga ttttaaacat accagctgct tccatgccgc tagatattat
     6181 ggaaaagctc attaggaatc cattatttac tacgatcaat ttgatcgtta ttgtcggaat
     6241 ttcactagtc atttataaaa aagaggggat gaagggatga attttaaaca ctcgtggaca
     6301 agacggttaa aatatgtttt tcccataatg atgatgttag gtacattgtt tgggttaaaa
     6361 acagccaatg tctttgctga aaaagttatt gtcgatccgt caaatccaat ccagaacgta
     6421 aagaataatt ttattattcc aaaagatgtt cctaatgcaa aaacaaatat tactgtaaac
     6481 ggagcgtcaa aagtttacaa atatcatgtt gatgccaaca aaggtggttt tacagacgac
     6541 tacgtaggat tagtggaggg aaacaaaaat atacgctatc cttctttcca aaaagaatac
     6601 ggtgaaacaa accttgtttt agaaggcgta acaaccaaca ccaaagtcaa tattgactat
     6661 ggaaaaatcg gtacgtataa cgggaaaaaa gtcaatatca agctagttct atctaatatt
     6721 cacttatact cggatacgtt gccgtggaat ctactagata acaactatac aaaaactcat
     6781 tttcgagacg atggctataa aaatacgaat ggtgcaatga gtaaatctaa aaaaagaaca
     6841 gtactttgga tctcagacaa tcttttctca ggaattgttt accattcaac acaaatgaat
     6901 gtgcagctag tagccacata tgaggacggt agccccgttc aattttcagg agatacattt
     6961 atcagtttta actctttgaa ccctgcagga ggaaaaagca cggatcttaa aggagaatat
     7021 gcccactatg acaaaatgaa tacgacagat tggtatgtca gaaaagatac ggttctttca
     7081 gaatttaaat ctttctacaa caatctgaac gtggtaggtg gccacccagg aggaagctct
     7141 aagctgacac aagcggataa tgatttcaat aatttgcatg ataaattagg agatccgaag
     7201 tttggtcaag gtactgtgtc ctttaaaata tctgaagcaa atccgacgtt tgtgattggt
     7261 tcttctaatg ttcaaacctg gtttacttta tcttctgcga caatctttag tgttgttcct
     7321 gatcaacctg aaaaaacagg ggttgataaa aatggaaata acgtgaacga taaaatgttg
     7381 caagtaggag acacgattca atatcgaatc aaacagaagg tgaatcgatt aggagtggat
     7441 ttactagcgg tttacgatcg atttgagtta atcgataatt taccaaaaga agtaaattac
     7501 gtcgatgcac acgttgaaag tggtacaaat aaaaaattcg atgtatctgg tgaagtgacc
     7561 tatgacaaaa caaaacacca ggtaaaatat gcagcgaaag ctgatacatt aaaaaatcga
     7621 atgaagtata acggcgaaac ttacgaatta gtgatcaatg taaaagtcaa tgaattggcc
     7681 aatcaaaata gtgtagccaa aaatcaggga acgtcgatca ttaataaagt agaaaaggat
     7741 acaaatatca ttaccgttta tttccctaaa attccagtga aagaagtaca acaaaatgga
     7801 aaagatgtaa acggacgaaa tgacggtaag aagggtacgc cgacagctcc tttaaacgca
     7861 ggatctgaag tacaatatct agtcacacaa aaatggcaca caaaaggcgt agacgctgcg
     7921 tcagatcact acaaacaatt tagtattcaa gatccaattg aagctcgttt gacatataaa
     7981 gaaggttctg cgcaagtaat agataagagt aaaggaaaag atattacttc tgaaggcaca
     8041 ctaacctatg atagcaattc aagaacgctg aagtgggaag cttccgctga cttcttaaat
     8101 aacaatcttt tagatggacg agaaattcag ctgatattta cagctaaaac tccattacaa
     8161 tcagaaaaaa atattgataa ccaagccgta gtagcagtag ataatgtttc taataaaaca
     8221 aatgttgtga caattggggt tgatcctaac ttgccacaag ttattgttcc taaaacaggt
     8281 tctacacatt tagtaacgat tttagcggtc agcttactat tacttgtgtt agcaactttt
     8341 ggctacgcag ttctgaagtt caattaaaca aaaaaacaag gctgaaaatc agccttgttt
     8401 ttctatttga aaatgggatt ggcactaata aatacgttgt ctgaagaaag tgatccgtct
     8461 gcagaaactt cataagcatg ggataaacca acgatacttt gattgttgta aagcaagttt
     8521 gcgttatgag cagcgctatc tttccacata accatagcat ttactgctcc tgtatctgaa
     8581 aggatctcgt caacgtaaag gtagaaacta ttgatatttt ctcctttagc accgtagcag
     8641 cctaagcttt ctaagagatc ttggaaagta ccgccgccag gtcggtaatg actgaagctt
     8701 acttgtaatt cttttgcacg aatatctgca gcttgttgaa cgatatcatt gaattgcatt
     8761 ggggcaacgc cattttgttg acggtagcta tttaaccaag caagttcttt ttgacgcaca
     8821 tagttgttta aatcacttag tgagatattt ttgtatttga aagtgatcgt tcgattttgg
     8881 tcaacattat tgattacacg ttgtccattt ttatcaacgg taacagatga atcgtcattt
     8941 ctacacaaga agtagttttg atcggtcata tttttccaag aagtagagaa actatctccc
     9001 tcgtaaaccg tttgtgtttc agtagataaa acagcgtttg tgtcaacgtt tatatggttc
     9061 acagtgacgt tgtattgatg agtataggca aaattgattc cttgattact tgtaacgttg
     9121 tttaaagtca cagtgttgct agagctttct ctaggatcaa gaacaaaatt agtgaacata
     9181 ggtgcttcaa ttgtgatgct ttgtccataa acgacgggat aactcttact gtaattatag
     9241 agttcttgga tatttggatc agtatccgag actttatttg gacgagcttt cacttcaact
     9301 agaaagactt catcatagta aaaagtaaat gtggtatttg tgttaatcgt ttgtgtttgt
     9361 gtttcattcc catttacttg atactgtggt gcttcaaagt tagtcaagtc ggttctagct
     9421 tttgcagtgt aacttgtacc agcttttact gagacatgtt cttcttttag cagcgttcca
     9481 gtttgtcgat tcatatgacg gacagttacc gcatattctg aaggtttagg tttgtgttgt
     9541 aaccctttaa atgcagaatc aagactattt ttagcattat ttacttgggt ttgagaagct
     9601 ttagaatcgt ctagtacttt tttagcattg gataaagcag tctgaaaatt attccaagat
     9661 ccttctgtaa aatctccttt agcagttgat tttacttggt tgtacaaggc ttgtaattcc
     9721 gttttgttca cagtaggagc tggttttttc tttaatccct tgtaagcaga atctaatgtg
     9781 tttttggcac tatccacttg tttttgagtc gcttttgagt catttaacac ttttttagca
     9841 ttgtttaaag cggtttggaa tgcgttccag gtattgtcgg tatagtcacc ttttttagtt
     9901 cccttaacgc tcgtataaag agcttctaaa gctgatttat tcactttggg tttagaggtg
     9961 tcagcaccat accaaccaat accttcgtat ttccagccat agtctttggt taaccattgt
    10021 acttctttcc aacttgcagt gtagtgatgc gaaccagagg taacacctgg aaggaaaagg
    10081 cggtagatcg gtttattatt tttttcggca gaacgccagg caggcccttc ataagtccaa
    10141 ccgtaatttt tggtcaacca tttcacttca tcccaactag ctgtgtaatg gtgatcacga
    10201 aggcctttat tgtataaacg atagacaagg tggccaccgc tagtcggtgc ttcccaagcg
    10261 gggccttcat aattacccca cttaattttc actaggtgat cacgttcttt tgtgctagag
    10321 gtgtaaaaat gttcatggtt atttggatta taaaggcgat acatgacgtt agttgaagca
    10381 gcgtcagcag atccagcaag agcaactgtg gcagcaaatg ttccaatagc tataacagtt
    10441 tttgggttta ataatttcat gttttcaact ccctattaaa tgattaaata ttttgtttac
    10501 attcataata taccacggta taccgaggta gtcaacggta taccgtggaa attttgaaat
    10561 aaaaaagccg ccctatgaag agtgtaagga tggctgttta tatgctatga agaaatggat
    10621 tcggcgtatt tggttagttt ggcagcatta tcaaatcgca ttttttcaat cgcacttttt
    10681 ttctttatta aatccgcaat tgttgattct gcgactgaag aatgtttgct aatttcatat
    10741 gctgtcttgc cagtttcaaa tagtaaccat tcgattttct ctttatcagc gtacattgca
    10801 atgctccctc ttgatttttt ctttattata gctctttttt gtaataaatc acaatatcaa
    10861 gaggaaaagt ttctatagaa aggtgggaaa ttatgagtat ccgttccccg tgtatcaaaa
    10921 tgaggtttgt tgctactttc gttatttaat atactaaaat aaaacaacga aagaaggaat
    10981 agaatgaaaa agttaataat ggtatttact tcgttgattg ccatgattgt tattttaggt
    11041 ggatgtggta gtgaaaaaaa taagcaagat agtcaagata atggcgtgtt aaaaccagga
    11101 acagtgtgga aagaagaagt aggcggactt gttcataatt taaaaattat agatgataca
    11161 acatgggaat attccgaaag tgtttggcat cctgaacaag tacaaataac agttgagaag
    11221 ctaaaagact ataaaggatt agaaagatat aaggtaatcg attcaggtgg agtagattta
    11281 ttcataggta aaacagcctt aatagttccc tataacaaag acggtttaga aacgtttaaa
    11341 tttattccaa caaacgaaaa agaacatcga gataaaagtg aaatattaaa agaatacagt
    11401 actaaatctg gatacaagtt acaaaagact agcgagtgat tgctagtctt tttatttatc
    11461 ctgaaggagg aatcgcagtg gcaaaagcta aaacgccatt tgaaaaagcc gcgtctaaaa
    11521 tcttagaacg tcgcggtgta tctgaaacac agtggaaaca agaagtcctt gaaaaagcac
    11581 aatttgacct attacgggga aaagacaagg acttagaagg atatatctat caacgtgaac
    11641 gtgaaaagct tgttttcgat gaaattatta aacactaaaa ggagcgaaca attttatgtt
    11701 tgaatcaatt acaatgaatt ttttaacaca accgaaagta gtagctgcgg ttgatatttt
    11761 tagagcatta caaggtgcag caactagttt aaaaaacaat ctgatgattt ttgcggctgt
    11821 agctgccgtt gcctccattg ttcttggggg gattttcttc ttctttggcc gaagaggagc
    11881 tgaaaacggg aagatgcacg taggttatgt attgatgggg gtaggagtta tattagctgc
    11941 cgcctctatt gtggtttcgt tatttggaat gtttggacaa accccacagg gactataaaa
    12001 aataaataga aggagtaaaa agtacgtggc tactttttac tcctttttta tgaaaggaga
    12061 atactcatgt actttacgat ttcaggcaat accaaaaaag ataaagagtt aataaaaaaa
    12121 gcaacgaaga aagtgggaaa gcgtactgcc tttttaccga aaacaattaa atattctgtc
    12181 attgaaacgg aagaacagct acatgtgtta ggacaagtgt tagaacaaga gaatcaatct
    12241 tctgttaaat tgactctcac gttacatgat aaagaaggaa aaatagaaaa ggctggcaca
    12301 gaatacgttt ttggcccact ggattatcaa atttcagaag gtttttatcc tacattgatg
    12361 gatttgatcc gccttgatgt gtttaaggaa acggatgaaa ccatgagtgt ggaagagtct
    12421 tatcgaaacg aaaaactgct acaagtttta gaagaagagt tagagagcga ccaaagaaaa
    12481 gtgagtcaag tgagtgaagt cgaagtaaat gatgaagtac catgggaacg accaatcttt
    12541 ccaaagaagg agtcaaaaga aaaagagaca gtctatgttg acgaacaagc tgatattccg
    12601 actcagaaat cgatatttga ggaagagaac gatgtaatgg gtcaattgga ggtagatccc
    12661 tttgaagaaa caatggagga tgaagagacg gccgaagtag aagttgatct agttgaagct
    12721 aatgaagagc caacatggac tgaaggggat tctcaggaat atattgaacc acttatcagt
    12781 gaagaagtag agaaaaacga aacagagata caaaaggcaa ctgtatttga ttatattttg
    12841 gatcaatatc cgccagagca tgaatggatg aaagaaaaaa ataaattatt tttaaatgag
    12901 ctttattcga aatatcaatt accccaaacc tatcaagcat ttttagagag taaagaagaa
    12961 ttaatctcac agggtaaagg cacactttta gaacagttag agtctgttca atcaacaaat
    13021 tggcatgagc ttgcacaaga agatttatta gccacttttg aagaacgtca acaagaaaca
    13081 gatcaagaaa taaatgagta tgcgacggaa cagtcatcta attgggagag aacaaaagag
    13141 gaactaaata aagaagaaca acgagtgatt gaagaagaag tacaaaaaat taaagagcga
    13201 tacgaaaaga aacgcgacaa tgcgtattcc ttgtgtatag agaaaattaa taactttact
    13261 aaaactaatc aagaagcttt atcgaaagaa aaagaaaact tgttaaaaga acgtataaaa
    13321 gaacgaaaag aagagttcta ccgaggttta cgtacagata agctaaagat tagcaaagaa
    13381 ttaaatgaac aactgaataa actttatcaa acaacgactg aagttcttat ggaaagacac
    13441 gggcagatcc agcaagaatt acaagcacaa attccaaaat ggaaaaaaga gtatgaaatt
    13501 caacaacaag aattgaaaga acaagaagaa aaacaacgac aagaagaaaa agagcgcgaa
    13561 caattagaac taaaaaaacg tgagcagcat cttgcagaac aaaaagtggc gatagaaaaa
    13621 caacggttag agcaagaaaa ggaaaaagaa gcagctgctc gtaaggaacg tcaagaacaa
    13681 atggatacat tgcgacaagc acagttgaat tatctagcac aacctcaacc agtagctgct
    13741 gttcaaacgc cagtgaacaa ccaagcaaat ttaactcctt caccacagcg ttcctttaac
    13801 ggatggatgg tgggatgtat tgcaagttta agcttgcttc taggtggagg aacagtattt
    13861 gcttttaatc atatcaatca atcttcacag gcagattctg tagccacaac ggcgtcaatc
    13921 aaggaagaag cccaggaaca agcaagaaaa gagtatgaac aacaattgga agaattattg
    13981 aatcgtacca atcagtcaac agcagagtca agtatggaaa atagcaaaga gagtgatcct
    14041 agtaaggcta aagaaggatc aagcgattca actgtacact caaacacttc cgaaagttca
    14101 gaatcaaatg aggtgaaata atgcgtatta gagaaacaac gagacgaaaa gacaaacgat
    14161 tcaatttaga aaagaatagg tatctttctc atttctatcg ctatactcct tcaggagaag
    14221 agctaccaga aaaacatgac aaacgtattt ctcaaaagat tttttatagt ggcttagctt
    14281 atcttccttt aaactatgtt gttacaagaa taccagaagc atttcatcga tttgagatta
    14341 ttccaggaag tttacaagga gtgaatattt ttcagttaca agaaatgccg cagttattcc
    14401 attttgattc aactggtttt tggaatcctt tgaccccaac aacaagttta ggcacacgtt
    14461 tagttgtttc tacgatttgt gttgttccag ctgtacttat gggattgaaa atggacatgt
    14521 ggtttcgtcc gctagcagat gggcaaaaag gagataaccg attaaaaacg ttaaaggaaa
    14581 tccaacaaat ttatccgcaa ataccagaat caaagggaag ttttcctggt tatggcggaa
    14641 taccaattac tcattataaa aaatactggt atattcagac ggatacagta aatacttgta
    14701 ttgttggtac ttcccgctca gggaaaggcc agatagaagt tttagcaacc attgacaatt
    14761 tgtcgcgtgc agaaagacaa agttcaatgg ttgttaatga tccaaaaaca gaattatatg
    14821 ttgctagtaa agatgaacta gaagctagag ggtatgatgt gtatgccttt aatatcaccg
    14881 atccactgca atcaatgagt gataatccat tggcactcat tgtgaaatac tggaagcgag
    14941 gagacataga caccgcaaca cagttaacca atacttttac gaatacgatt tattatgatg
    15001 caaatgcttc agacaataaa tactttaacg aaaatgcgca gaaagctgtc aatgcgctta
    15061 tttttgcgtt attggaacaa gcggatcaaa caggggatta ctccaaatta acaccaaata
    15121 atctcgttga actactttca gaaattggcg gttttaatta ttatccagat cctgagaatg
    15181 aattcaagca gctcaatgcc ttagatgagt tcatgaatca gttaccccct ggaaatatag
    15241 caaaaaaaca atatggggca acaaaaattg gatctgataa agcgaaaggg aatatcttgt
    15301 ctacggcaat tacaggattg aatccattta ccctaacgaa aattgcgaag atgacttctc
    15361 aatcgaccat tgattacaag tccattggtt ttccgaagta tttggatctc aaattacatg
    15421 aaagcctatt aaatcaacgc attgtcgtgg aattttatca taagggaaag aaatttcacg
    15481 aggaacgagt caaagttggc tatcgtggtt tttgtgaaat caactttgat tgtcatttag
    15541 agcagggaga tcatgtttgc ttaaaataca atcatcaagg aaaacaatat gttgcttggt
    15601 atgagttttc tcaaaaagta ttgaaagacg aagagggaaa aatcgtttat cgaaaaaaac
    15661 gaggagaaac tcatttgcca gaactagaaa aagaagccgt tctaacatta gatgagaaga
    15721 aaagtaacct attagtagag tctattcgga tgcactatac cgataaacca atggctattt
    15781 ttatgttaac gcctgattat gatccatcta accatatcat tgttagcatc tttcttagtc
    15841 agttgtataa agaattatct acgcaatgtg ttaagactaa aggagataaa tgtcaccgta
    15901 ggattcattt tattttagat gaatttggaa atatgccacc gatggacaac atggaaggaa
    15961 tcatgacggt tacggcaggt cgtaatatgt tatgggatct gtttattcaa tcttatcagc
    16021 aactttatgc gaagtatgga gaacaaaacg cagcgattat caaagaaaat tgtcagacac
    16081 atatttatat tatgtcgaca gatgataata ccatcgaaga attttctaaa aaagtgggga
    16141 ataagacggt tgaacaagag aacagtacca tcaattcatt aggtatgaat actcatatca
    16201 atcgcaatgt tgattcagat cgtatcttaa ctcctgaaag aatttctact tttttagaag
    16261 gtgaaacgat tgtactcgct cctttaaaac gacaagattt gaaaggtcat aaaatacggc
    16321 cgtatcctat ttttaatcat ggggaaacga atcttcctta tgcttatcaa tatattgaag
    16381 atttcaatcc atcgtgggat atcaatgata tagacataca ttgtacacat gcggacttgg
    16441 atcttaaggc tttacaaatt gattatgatt atttcctacc agatgaatat gctagaaatc
    16501 tctatcatgg actgcatgat caattagaag aaaaaaacga agaaaatatg ttttataaaa
    16561 tcgttcaatc ttttgagaat gagacggcta aaaaattgtt aattcaattt tacgaagaac
    16621 aagcaacgga tcgtcttcaa gaatatgttg aaaagttgat caaaaatgaa gaaatttctg
    16681 aaatgattag gaaacggctg ttattggaag ctgaaaaggc ggtgaaagaa tgatcattgg
    16741 aggaattttc tctggcatag ccgactggtg gtcaggttta ttcggagaag gggatgcaga
    16801 tcccaaaacc gtaacgaaag tcttacaaga ttatggtaat tatctgcagt atcaagatgt
    16861 attcagttgg atttgtaata cttttttatg gggattgatc aaagggttat ataatctcaa
    16921 tactttatta gaaaaaacaa tttatcaaag ctttgattta aaagacttat tgaacgccgc
    16981 tggtgttaat gaactatttc aaagtttgat tagtaaggta ttggctcttt tttgtgtcgt
    17041 cacattaatt tatttaggct tgaaattttt tatgagtaaa cacccaccga aagtcaaaaa
    17101 tatcttggtt aatttattta tggccatgat cttgattttt ggtggtagtt ctttaatcga
    17161 tgaagggtta aatatcagta aaaactttta tggagacgtc actgcagcaa ataaagcaga
    17221 taaaacgggt tcacctgctt ttcaaattat tcaaaataat gtgattgatg tcagtacatt
    17281 actagcgact gacccgaata aagtagaatc cttaccaaca gataaaagaa atgctttaac
    17341 agcgaaaaat ttcaaaacag cgaatatcaa tgggattatt acacctgatc aagctaagaa
    17401 aatgtctgaa tcaagtgata ttagtacagt acaaaaagat cggttaaagg cattacagta
    17461 tcgactagag ttagacgata atgggaaaga aatccctgtt caaattagtg atgaaggttt
    17521 agcaaaatat gtgtatacgt caggctatcg tcgatacatt agtagtccag ggattatttt
    17581 ggctggggaa tcttctttgt cggtagccta tttatttatt ttgtttacaa tcgttacttg
    17641 cattattgag ctagtattta aaaaattcta tttagtaatt gccgcgtcaa cggactttga
    17701 gacaggacaa cggatgaaaa gtgcgataga agatatttct caatccttct tgttattggc
    17761 gtttacgatt ttagaattac gaatctatac attaatgctt tcaggtatcg gtgatttgca
    17821 tgcggtagga aagatcaatg gattcttgta tgttgttgct ttaattgtgc taactattgc
    17881 gctattcaaa ggctctcaat cggttacgaa gattttcggt gttgatacga gtctgaaaaa
    17941 cggcagcaat tctcttctta gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag
    18001 cggagctaaa aaccttgcaa gtgctggtaa gagtggttta agccatttaa atgatattcg
    18061 taagaatgga tttggccatc gaaaagatat tacccatgat aacggggaag aacaatcaga
    18121 cgataaagct acaactgtac ctaatagtaa gaagacagtt tccgaaatgt tcgggaaagc
    18181 gaaaaatggt gtttctaaag ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa
    18241 aggaacagtt gaagatctta cagaaaaagc caaagacacc ttagaagaga aattagacgg
    18301 cacgagagct aaagaatttg ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca
    18361 aaaagaaaaa gcttcagcag tcaaagaagg actaaaagat gcgtatggtg aaggacaagt
    18421 caatgcagca gtgaaggcaa atacaacaag tgaaaaagaa aatcaagaag gtgcagcgat
    18481 tccttcagcc aaagatcacc aagatgagtt acattctaac gaaaattcga tcggagaaaa
    18541 agattcagaa gaaggacaac atggtcagtt accaaaaacc gatccagaac aatatcgtta
    18601 taaatctaac attaattctg aaaacgaaaa acgcatttca gatttaccaa aacgaaatca
    18661 agaagtacaa acggatgagg attccatcga tctgccaaat gataatggca tcgaagttgg
    18721 aactgaaaaa tcaacgaaaa ttggtttgcc taagacacaa aatttaccaa tgaaagatag
    18781 tcgtttgaat caattcaaag agaatgatca aagctttgtt catcaatcac ctgcaggtac
    18841 tgttcaaatg aaatcaagtc gtttgcctga aaatggaaat ctgacaactg tttcttacaa
    18901 tttagctgaa agtggtgaaa cagagaatgc gtccgttcaa aatattgtga caaaagaagc
    18961 tgcacctaag ccaatcaata aagttcataa tggatacaga ccagcaaacg tagcatttac
    19021 tgcaccgaca aaaataaatg tgacaagaga aactaaaaac aggaacaatg aattgaaaga
    19081 acaactattg aaggatatca aaccataaaa ggcctattta agggcctttt tctttataaa
    19141 acaagtttaa aatggaaagg agaaaattgt ggaggatatt ttaatagcta cgtttataac
    19201 tgcactggga gcatttttaa cgatatttgt ggtgactatt aaaggattta aagatacgga
    19261 tagtttacaa aaagatcatc agtctatttt agatggtcaa aaaacacata ccgaaagcat
    19321 tattgtacac ctttcagaag gattcactta tttgaaagat gctatgccca cattcacagt
    19381 aaaattaacg gaaatatttc attctgttga gcaaatatcg tcagaattga aagagcaaag
    19441 acaacaaaaa gatcgtcaac ttggctcact taattttagg caagaaacga tacaagaatc
    19501 attggagaat gtaaatcgat taaacgatga aatgcaacga gttcatttgg aaaatgaaga
    19561 attacgcgca caaaattatg atttaattca aaagaaaaga aatctggaac aaaaaattgt
    19621 tgaattaaaa gaagaaaatc gtcaattgaa ttatcaatta cgacgaggaa aaaatcgttc
    19681 tcgtgattta ggaaatgaat tagagttgta atatttagaa aaacgctgtg agaaatcaca
    19741 gtgtttttta tttctaaaaa tacaaaatat aaggggtatg gttatattat ggataaatac
    19801 attcatatga aaaatcctcc aagatttagt ggggaatgga agtttttcgc ctttttcttt
    19861 ttagatctat taacgcttgg tggctgcttt ttttgggcga aaatagtcaa taatcttgtc
    19921 cacttaccac tcttttttgc actcttgaat tatggattat ggcttgcgat agcgttagtg
    19981 attgtttggc gaccctataa taaccctggt caacgccaaa tttttgcgat tatcgaatct
    20041 attttcttta cggataagaa ccactatcat gcgttagatc ctaaccgaaa ttatcatggt
    20101 acgtataagg aggatgaata aatgcgagtc aaaggacaaa attcagttga taagaaatcc
    20161 attttgcagt ttggaaaacc agaagagcgc tctatcttgg acttatttcc atataaatcc
    20221 tgtacagaag agggagtctt aattactaga gaagatcatt ttcagcgtta ttatcgtgtc
    20281 gtttccactg acgtggaggg attaaatgaa caagagaaac tagaacgaat gaatcagttg
    20341 accaccgtca tgcgtacgta tgtgccaaca attaagttag ttagtttgac cacagagacg
    20401 gatctaacgg aacaaatcgt gcaaaagcgg cagttattag ataaaaatcg tatggaacag
    20461 tcactaggaa gaaatctaca gcgattaaga aaatatgaaa agaaattagt agaggagatc
    20521 caggaattaa aacgggcaga acaagaacgt cctgacttaa gcttcttttt cgttattgaa
    20581 ggaaagacaa tcaaggattt acaaactaga aatcgtcagt taatgcgatc tagtggcatt
    20641 ttagggttaa aaccactcta caaaaaagaa ttgattaaga ttctttatcg aatgaacaac
    20701 atgaatgacg aataaaaaag atggaggaaa tgattcatgc tagatgtgat aaaaacagct
    20761 ttttcaacgc aaagtaatca aaaggtgatt gatccattaa aacaacttgg taatcgtgaa
    20821 atagagattt taaaatcaaa aggttatgat ttagatttct taaaacaagt gatgccacaa
    20881 ggtgggttga attttgatga agaatatgtg acctatggaa atggttattc acgatgtgtg
    20941 acagtttatc attacacaaa aaatcctact cttttttggt tggccaatct catgaacaat
    21001 cctcatacag tggccacact agatgttagc acagatgaaa agcaaaaagt gattgatcaa
    21061 atcaaccgcg cattagatga attaaatgat cgatcagata atgaacgaaa agcgactgac
    21121 cgaaataaaa gtgcggaaga atacctggaa ctagcggaat atgcccgaaa attgacttct
    21181 aatggggaaa tttctaaaca agtcaaagtg agagtacatg tttacggaaa tacgatccaa
    21241 gaagtggatg aacgggcgca ggaactgatc gaaaatctaa aagggatgga ttatcgtgcg
    21301 gtgacttatc tgcataaaac caaacgagat tggcaatcga tgtttaccat gttcgatgaa
    21361 caagctgaat ggtttggaaa tatcaaaggg caatcacttc catcgttaaa cattggcgga
    21421 ggattcccgt tccaccacca aacgctaaaa gatccaggag gcacacaata tggggaaacc
    21481 atgacaggcg gcccatttgt ctggaatgcc acgtataaaa atagtgtacg tttatctacc
    21541 aataccttgc ttctaggaat gatgggagct ggtaaatcga ccgttcttaa aatgatcgcg
    21601 gaagcccatt tagctgcagg agactttatt tggggctttg aaaagggaaa agactttatt
    21661 cctttcttaa aggaatataa cggtattatg gtgcgtttag acggatctga cgggatgatc
    21721 aatcctttag agatctttgc cacacggacg tatgatgaag catccagttt atatgacgat
    21781 ggttcagtca aagatttgaa gatcaatgaa gcagcgtcct atcaaacgca cttagacaaa
    21841 gtagtctatc aggtacagtt agtgagtcct cagctaaaag gaacgatgaa agcagaattc
    21901 aagacctact tgaatcgttt ttatgaagag tatggaacag taccacgcgg ttttacttcc
    21961 tcaaattcgc gttcaaatac tgatacgcaa gtcacaggga aagatccaga agcttatccg
    22021 acgttcaaag aatttttagc ctttttagga caactggaac taccaggggc ttcccaagag
    22081 aaaaagaatc ggaaagaaga aatggagtct atcgtggaat ccttatgtga gacttatggc
    22141 atgatttttg atggtcatag tacgattcga caccttgacc aacaacagtt ggtctttttt
    22201 gacattgact ctattacagg cctagatcga ggggtacaac aatgtcagat gtacatggcc
    22261 atgacgttga tttggaatca ggcattgatt caaggacgaa gacaacgacg cttaattaaa
    22321 gaaaaacaaa tggaagaaca agacttcaaa cgcttcttgg cgatgattga tgagtgccat
    22381 aatggtgcga cctgttctgc agtgaaaagc tgtgggttgc aagtagtcta aaaactattg
    22441 taataactct tataacgaga agtcaaatga gtgggtaacg ccacaaaggg cttccactga
    22501 tactccgaca tgcaacaagt agtttatgca gaccaacgtt gtgtgaagct cggtgaagtc
    22561 ggctgaagga taccgtaagg taaaagaaat agtgaatacc acgaaagttc tttagaggta
    22621 aagggtgtat ccgataagtc gggtgtccaa aaaaacttaa tatggtgagt atgtacaaac
    22681 gtactgacga actagcgaat gtactgtccg aaaggcatag ctcctagaaa tgggagagac
    22741 agtgagcaaa aaatcactca ttgtgctaac ttgtggttga gtaaaagtcg ttgttatgaa
    22801 agaccatgct gcgttacagg cgtaggagaa gttagggggc aaaagctagc atctaagtta
    22861 aggcatgtgg atagatataa gggaacaggg gaagccatca acatggagag tgcatactcg
    22921 atgaagtggt agtaagaaat atgtgaatga caaataactt actttagtga tggtgaaagt
    22981 agagtcacga ctgatgaagg ttctgtaatg gaactggagg aacaggctcg attcgtctgt
    23041 aaaagtcatg aaacccgttc aaccatggga ttctcgtatg actaaaaagg tcacgagcca
    23101 attggagcgt gataaaccat gacaaaagaa acaagtccaa aagataggaa gttgcggcat
    23161 gcagaatatt atggcatgac ggaaatcttc gataagatat actccaaaag tttgaaggga
    23221 gataatttca agaatctaat gcagattatc atttctgata acaacattgt cctagcatat
    23281 cgtaatatca agagaaatca cggtagtgtg acaagaggag aagatggagt aacgattaaa
    23341 gatattgaaa atctcaaccg taatgaattc gtagaaattg ttaggaagcg attcgcacat
    23401 tataaccctc ggaaagtaag gcgtgtagaa attcctaaat ataatggtaa attaagaccg
    23461 ttgggtattc catcaatatg ggatagaatc gcgcagcaat gcatcttaca agtattagaa
    23521 ccaatatgtg aagcaaaatt caataatcac agctatggtt tccgcccaaa tagatcagca
    23581 gaacacgcaa tagcagattg tatggcccga atgaatcaaa gtcacatgga atatgtggta
    23641 gatgtagata tcaaaggttt cttcgacgaa gtcaatcata ctaaactcat gagacaaatc
    23701 tggtctatgg gtatacgaga caaacagctt ctcttaatca ttaggaaaat gttaagagca
    23761 cccgtagtac ttccaaatgg gaagttgata tacccaacaa aaggaacacc acaaggcgga
    23821 atacttagcc cattacttgc gaacatcaat ctcaacgaat ttgactggtg gatagcaaat
    23881 cagtgggagg aaaaagatgc caaagagcta aaacatcatt ttaggaaaaa tggagcttgg
    23941 gataaaggta atatctatca caagctcaga aagagtacaa ctctaaaaga aatataccta
    24001 gtgagatacg cagatgattt taaaattttc tgtagaaatt ataatgaagc gaaaaaagtt
    24061 ttctatgcaa ctaaaatgtg gttacaggag cgtctaaagt taccaatatc tgaagaaaag
    24121 tcaaaaatca caaatctttc aaaagaagaa agtgaatttt taggctttac gatgaaatta
    24181 gtgaccaaag gtaacaaaaa ggttgttaat acgcatatag gtacaaaagc acaaagcatg
    24241 attaaagtga aactaaaaaa acaaatcaaa cgaattcaaa aaagtcctaa tagcaaagat
    24301 acctttatct atattggagt ctataacagt atggttattg ggattcataa ttacttcaaa
    24361 attgcgactc attgtagtgt agcaatgcga aaaattgcaa gagaactcag accaagtttt
    24421 gaaatacgac tacgagaaca aggaatctca aaagaaggac aatacggaaa gtacgaagga
    24481 tacaagcctt acctacggtc aggacaaata cgatatataa gagggtttcc aatattacca
    24541 gtagggtata ttcaacacag acatccgatg tgtaaaaaaa gaaatattaa taaatacacc
    24601 gaagaaggta gggaagccat tcataagaag caacaaaccg tagaagaatg gaagatatcg
    24661 tggttgtgta gcaatccagt gcttagtaag agagcaacga ttgaatttgc ggacaatcga
    24721 atttcaaaat tcatcgctca aaagggaaga tgtgcagtaa caggtgagga gcttatctta
    24781 tctgaaatgc attgtcatca tattatccct tatcatgaaa gcaaaagtga tagctatgaa
    24841 aacctagtga ttgttactga ggaggttcat cgggtaatac acgctacaca aagtgaaacg
    24901 attgaggaac ttctaaaata tctaaaatta aatccaaaac aaaaagaaaa actcaatgag
    24961 ttgagattaa aagttggtaa cgaagaaata tcttaataaa tatttgtagg acgggaaaac
    25021 agaatgtctt ttatagacga gggaacgccg tatgcggtga aagtcgcacg tacggtgtgg
    25081 actgggggaa aagacggaga taatatcaaa ctcttaccta tcagtatcga ttaatccaga
    25141 tttcattgaa acggtaaatt atgtcacaaa cttccaacga gaaatgcgta aagtagaagc
    25201 aatgactgtt ttagctacac aatccccaca agaaatggtg ccagaaaata tgtcttctga
    25261 taacttctcg aaactgaaga aagtctttga attttcttct acgaagatct ttatgcgtat
    25321 ggatgaatct attttacgtc atatagaagg attagttggt aaatcaatga cgaactcaga
    25381 attagaatcc attcctcaac aaaatcaagg agacgctgtg atcaattttg gctcaaaaga
    25441 aaccattcgt gttcatttta gtccgaacaa acgtcaatta aaattgtttg atggaggaaa
    25501 ataatggaaa accaaaatga aagtttaatc aaacagtatg ttaagcgtcg tgcaaagcgg
    25561 cgcttatttt tgtgggtgtt tggtacttct gcaggtttga ttactatatt gatcactgtg
    25621 tttgtgaccc tttttctaat acttgcggca ggttctattg ataattctga ttcaagtagt
    25681 ggtggcgaag cttttacagg agaatattcg gaaggcttac ccatttataa agaaatcaaa
    25741 ggaagaggcc ctttttctga tgagattgcg caatatgctg taggtgcagc agtgaagtat
    25801 aaattattac caagtgtgat cctttcgcaa tacggttatg aatcagcgtt tggtacgtcg
    25861 gcgtccgcaa gaaatgatct caattatttt gggattacgt ggtttgatgg gtgtctgttt
    25921 cctaaaggaa ctgcgcgtgg aattggtggc attgaaggtg gctggtacat gaagtttccc
    25981 aactcaaaag ctgccttctc ctattacggc tttatggtag ccacacaatc aaattttaat
    26041 gcttgtgtcg gaaataaaag ccctggcgca agcttattga tcttaggacg aggagggtat
    26101 gccgccgcag gaattactga agacagtgcc tattataaaa attgtatgtc tattattaat
    26161 acaaataaat tgatggagta cgatgagttt gcgattaaac actggagaga aggcggcgga
    26221 actggtggta cgattacagg aagctggggc aatccattcc caggaagtag cttagataaa
    26281 aattcgttca gtggaggaca actttttggg aaaaatccag gaggagaatt ccgacctaac
    26341 gggttccatg atggactaga ctttggttca gtcgatcatc caggaagtga gattcatgcg
    26401 gttcatggcg gaaaagtcgt gtacgttggc aatcctggta tttcaggttt gggtgcgtgt
    26461 gtgatcgtga ttaattacga tggcttgaat atggtgtatc aagaatttgc aaatagtaca
    26521 ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg gtcaagtaat tgggatacga
    26581 gatacagcac acttgcattt aggatttact cgaatggatt ggcgtcaagc acaaggacat
    26641 gcattcattg atgatggtac atggatcgat ccgttaccat ttttgaatag cagcaaaaaa
    26701 tagatcagga gaaaaaatat ggaaaataaa accttaaaaa tcgtcttaac agctgcaatt
    26761 agcgttagtt taatcggcaa tattgcgttg ttttggcagc ttaaagaaga acaagagaca
    26821 aatacctata aaattgaaga agtggctaaa aacgcgtctg ataagctcaa aaaaaattct
    26881 tctgaagcac aggcgaagat tgacaagtta aatcaaacca tcaatgagaa aaaccaagaa
    26941 atcgatcagc tgaagcaaca agatggcaca aaagcaaaag aggaagtaac tgaagcacaa
    27001 agaaagacgg cagaagagtt cggtcaattg gccattgata aatcattaag cagaaatgaa
    27061 ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga tcgacaaatt aagtcctgta
    27121 gacgatgatc accaacatga tgattacgga agttactctt ttacattagg tgacgtgcaa
    27181 acttacgctc aaacaagcag tgtaaaagag gaacaaaatt ttgtcgtatt tgttgattat
    27241 acaatcggca atcctcaatt caaagatgtc aaaccacaaa aaataaaagg tggattaact
    27301 gtcactgaaa aacaagtaga tggtcaatgg aaagtcacag actttagtta tttcactcgt
    27361 taggaggaag aaaatgagta agaaaaaagg agcaagacaa cgttttaaaa aacgaaaaag
    27421 ttatcttaga tggctgggga tctttatgat tctcggcttt ttgattcttg ctagtccgat
    27481 ttacacacat aaaaaggtag acataccaga tgaagtaagc acgaacgcat ttttaaacac
    27541 gaaggaactg acgatggttt caaaagaaaa aggagcgaac cagcaattag tttttgaatt
    27601 tgcgatcgac gatgaagatc agagcttatt aaaagaacta gctaatctta attatcgtgt
    27661 agaagtaaag acagctaaag gggattatca agcaataaaa gccaaagtga tcaaggtgtc
    27721 tgatgattat tttgtagtga agttagcaca tgtaccagaa gaatatattg ctatgcgatt
    27781 aactattatt cctgaaaaaa ttgatccaaa ggtggatatg caagaaccgc aagatctgat
    27841 tttttatatt cacgaagata aggtcaaaga tcacgtgaaa gacgaagatt atgaacaaca
    27901 tgcgatcaat tacaaagtga aaggatacaa gaaagaacaa aaagcagcac aaaaacaaat
    27961 tgaatccttc caagcaacca ttgatttgaa tgaagaatta gtgaagaaac taaaagatca
    28021 attgccttat caagtcgcag aagatcagga aaatactgaa aataagatta atagttacca
    28081 gcaagaaatt gaaacatcga aaacgcaaat gaaagatcaa gaagagagca ttcaaaaact
    28141 tcaagaaaaa attgatcgta tgacaaaaga aaatatttag aagaataggt ggagtaaaag
    28201 aaatgaagcg agcgaacttg gtcggcaaag atactttcgc tgagcagaaa gtattgatta
    28261 aagcctatct caaagttgta aaagctaaag aaatatgtca agaagtgaat tatctaacac
    28321 aacaatatct gagttgaaga atggtagaaa gagtttagat accttaaaat tcagatcatt
    28381 tgaagctcta tatatagctg ctaaaaaata ttttacagct gataataaat aaaaaattaa
    28441 ggtgggttct atgtgaaaaa aacgtatcaa aagtttttat ggattagcta tatcgtccta
    28501 tttatcgttg ggtttctcgt cagtgtgtcc gtgatcgtcg gaacgctttt tcttttgttc
    28561 tctgacgaag atctggtact taaggggtta atgagtacgt tatcgtgtgt catgttactt
    28621 tatttatgta tacagatcaa cgaaattatg ccccctcatt ggcaagaaaa gaagaaagaa
    28681 tttaaggaag aaatcgaaat aaaatccaat gactggattg aatacacatg gcttcctctg
    28741 aaaagaaata gcaaacgaaa ggaattttgg gcattgcaat tatggccaat aggaaaactt
    28801 tttatttata tggcttcact tatggttact ttaatcggtt atttggtggt ggtctatggg
    28861 gtggcctatt acttgttaag tgagccaaaa cagatcgcgg cgtcaaatat tttcacggca
    28921 gtaatgacat taatgtgtgt catgtgcttt tctattttta gttacttact cgtccaattt
    28981 atcaggcaaa atttaacaat gagtaaactt ttcaaaagtt tattggactt acttatctta
    29041 ggcagtatgt taacgactct aacagatcgg ttcgaacaat ctgaaaaaac ggtagagggg
    29101 ataaagaact tcttaacgac gattgatcaa tttataaatg gatatatgta tttgtttgaa
    29161 gtaggcttta ttctcacttt gtttggttgt gtatttataa aactcattaa aagatcaatc
    29221 gaaaaagaaa tggttattga tctaattccg acgctgagcg gctatgagta ttacgttgat
    29281 tttggaaagt atgaggagtt gatccatacg gatatcgttc acacgagtat ccatgaagta
    29341 accgttaagg gcatcagatc tactttaaaa gatggacagg agacgaaaac aatcgaccga
    29401 agaaaagagg tctacaattt ttataaagtt gaagcaagtc tacgatatgg cattgggaaa
    29461 ttgaggttag atgggagtaa tattcaacaa aacaaaccat atgttttagg aaaagtggtg
    29521 atttagaact tcctatgaaa gatgaataaa taaggagaat cttttgaaaa tgattcttct
    29581 ttacagaatt gtgcttatag ttggcacgga gttaaaaatc ttaaagaagg agcagttatt
    29641 caaagtgaaa gaacatcata atcagttact atgtattaaa acgggtacga tgaacattca
    29701 ataccgaaca gaatatttag aattaacgaa tcaaactgat tttttgtcga ttcgagagca
    29761 acataagcaa gaaaaattaa tggctactta tagccaattg attgatgagt gggaacaatt
    29821 caaggaatct aaaacataaa attccattta aacaccttat tttttaaaga gcataaggaa
    29881 acgtgtaccc actttcaaaa taaagtgggt agataggctg tgcctatcac gtttctgaaa
    29941 aaaataaatg accccaaaat tttcaattta tgtgtaggtt tatgggtagc aaaaatatga
    30001 atgtgggcag caaaaagttc atttgtgggt agtaaaattc aaatgtgtgt agcaatgtgt
    30061 gtagctacac acattgctac acacgctata acatatagtg aaaaggagat atttatggaa
    30121 gtaaaaataa gagatattga tgaaaaaacg attcaaagaa ttgaccgaag atgtattgaa
    30181 ttaagtgaac gcaccgggac taaatggagc cgaaatcgat acctaaaagt tttgattgaa
    30241 aatgattttg accatgaact tatggatcat agaaaagatc aatttgatcg gttgttagaa
    30301 aaatttgtgg atattcaaac ttataatacg aaaattttag aagaatatat tgcaacaaat
    30361 aatcaattaa tcgccttgtt aattgaataa taaaaatagt attgtgaatt tataaaagga
    30421 ggaaatcaat tttggcaaaa gcaacaagaa aacatgtata tctttacgat atgcatatcg
    30481 aagtacttga tgaaataatt tctactcatg aagatatatc taattatagt gaagcagtca
    30541 gacatttgtg tatgatgtat gatccaaaaa tcgaagaaaa aataaatcca attcaaatta
    30601 aattgaatgc aatgggaaaa gaaatttcta tattaaccga aatggtttca gagtttgcgg
    30661 atcttcattt gaaagataca ggaatactaa ttggtacaaa ttcaactgtc tatcaagaag
    30721 caaaagagcg tgtggaaggg aaaataaaag cgaatcaaac gaaaaagtat tctcctaaga
    30781 tcaagaaacc aataaatgaa tctgttcatg ccagagtaga cccgtttgat ccaacaaagt
    30841 ttttaaaaag ataaaaagta gaaggggaac atgatgaata aatcgcctgc agtcattctc
    30901 acttgtcaat ttaccttgcc caataaaaaa tctttttcaa catacattga ttatatgaca
    30961 agagaaaaag cgctggaaga aaaggaacga cgcacgccgg acgaagaaca ggaactgcaa
    31021 cgaatcaaga atgctttaga ggatttttat attcctgaag gagaaacttt ttcggagagt
    31081 aagacagata aagaattcag tgaaacgtcg aatgaagctc aatcgttgat ggaacatgga
    31141 atcaattttg acaagttaga ggaacaagat tttaccaaat atctttctta tatgacccgt
    31201 cactatgcgt tagaacaaaa gaaagaactg acgcaaaggg aacaaaaaga ataccaacgg
    31261 ttgaaccaag caatgaaaaa atatcaaaca gagccacata aaaaagcttc aggaaatctt
    31321 cctggagttt tttctatggc ttcagacgaa gtgaaaggaa gtgatctgaa ggagatcaaa
    31381 caaatcttcc aaagagggca acaaaaaggc tcaatcattt atcaagatgt tgtctctcac
    31441 gacaattcgt atcttgaaaa aatgggcttg tacgatccca aaacagatac cttagatgaa
    31501 gaaggattaa aagcagctgg aagaaaaatg atggagacat tattcgagaa agaacaattg
    31561 aatgaaacag gttactggat ggcaacgatt caccgtaaca ctaagcatat ccatattcat
    31621 tttgcagttg ttgaaaaaga aaatacgcgt aaaccttaca cggaggttga aaatggtgtg
    31681 agttatatcg taccgaaagg taagagaaga caagcaacat tagacgcaat gaagaccaat
    31741 tatgtacatg agttagaacg tttttcttat gaaaaggatc gaatgattgg tatggagaaa
    31801 cgaaatttat tgacgcggaa aagtgatttg cgtaacacct taacaaaagt ggtaaaagag
    31861 ccagcgcgtt acgatcgaca cgcaatgaac ttattaaatg aagtatataa aaccctacct
    31921 gacaaacgtt cggaatggca ttatggggat gaaaaacgaa ccaaactttc accatacaca
    31981 agagagaaac tcgatgagtt aaccaaatat gtgttaacaa aagaacctga atatcaagaa
    32041 tatatacaga tttcaaaatc attgaaagaa gaagcaaaaa gtctttatgg ggaaagcaag
    32101 agagaatcaa aggatgcaga aaaaaatgct ctctttgatt tgaagaagcg cacaggaaat
    32161 gccattctaa cagaactgaa gaaacaggat agtcaaataa aacctttaat agaaagtatg
    32221 gatcagatgc aatctattta caatgaacga tcctttcgaa cgcttaaaca gttaccaacg
    32281 aatgaataca aatatagtta cgaagaattc atgaagcgca ggaaacaatt ccaacaaaga
    32341 gttgtcacgc aacggagttt gcgtcaatta aatcgaacga ttaatgccca tgaagaaaaa
    32401 taccacgcaa gtaaagccta cgaagagcaa caacgaagaa ttcaaatgga acaagaaaga
    32461 cagtaaagac agtaggagga atagcaatga aacaatattt agatcaatgg aaagttatag
    32521 aaggatcatt aagagaagaa cgaatcgaac aacttcccga ttgtttagaa aaagagcatt
    32581 tgtttcagat aagagaaatg ttgagaaacg aacagtttga tccaaatcaa tttttagtcg
    32641 ttgagtactc agctactggt gtgtactgtt gtaatcatgt gaagggtgaa aaatatttta
    32701 tcattcaaga atatgagggc aagttagcgc cttattatac tacttgggaa atgaatgaag
    32761 aggggatcaa taacttcccg tgtaaaagca ttgaagagtc tatttcactc acagaatgtt
    32821 agttgttaag tgaataaaag aagtaaaaga gtcctgaacg taattgtcca ggactctttt
    32881 tttcaatgaa ttgttttccg ccaattcaat gagtggtgcg atttgcgact acattatatc
    32941 aaaagaaaga gaagggagga agaagaaatg gcttattcaa cgattggagt tgttgttgaa
    33001 aaaagcagag acaatctcgt ttttgtgaca gaaattcaaa cgggcagagc ttttgtcgtc
    33061 acagacaaag cggcaaaagc gtatcaaagt ggcgatattt taacgttaaa tctgactacg
    33121 aagacgttcg tcgatgcggc agaagattat ccatt
//
LOCUS       DACEMO010000009        31434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEMO010000009 DACEMO010000000
VERSION     DACEMO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.009"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..490)
                     /locus_tag="IW728_000324"
     CDS             complement(23..490)
                     /locus_tag="IW728_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1023708.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(610..2148)
                     /locus_tag="IW728_000325"
     CDS             complement(610..2148)
                     /locus_tag="IW728_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1023709.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2228..2914
                     /locus_tag="IW728_000326"
     CDS             2228..2914
                     /locus_tag="IW728_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1023710.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3115..3642)
                     /gene="msrA"
                     /locus_tag="IW728_000327"
     CDS             complement(3115..3642)
                     /gene="msrA"
                     /locus_tag="IW728_000327"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1023711.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3726..4403)
                     /gene="pgmB"
                     /locus_tag="IW728_000328"
     CDS             complement(3726..4403)
                     /gene="pgmB"
                     /locus_tag="IW728_000328"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1023712.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4400..5530)
                     /locus_tag="IW728_000329"
     CDS             complement(4400..5530)
                     /locus_tag="IW728_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1023713.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5530..7713)
                     /locus_tag="IW728_000330"
     CDS             complement(5530..7713)
                     /locus_tag="IW728_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1023714.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7710..8735)
                     /locus_tag="IW728_000331"
     CDS             complement(7710..8735)
                     /locus_tag="IW728_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1023715.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8780..9607)
                     /locus_tag="IW728_000332"
     CDS             complement(8780..9607)
                     /locus_tag="IW728_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1023716.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9604..10671)
                     /locus_tag="IW728_000333"
     CDS             complement(9604..10671)
                     /locus_tag="IW728_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1023717.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10697..11533)
                     /locus_tag="IW728_000334"
     CDS             complement(10697..11533)
                     /locus_tag="IW728_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1023718.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11530..12399)
                     /locus_tag="IW728_000335"
     CDS             complement(11530..12399)
                     /locus_tag="IW728_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1023719.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12469..13752)
                     /locus_tag="IW728_000336"
     CDS             complement(12469..13752)
                     /locus_tag="IW728_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1023720.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13766..15451)
                     /locus_tag="IW728_000337"
     CDS             complement(13766..15451)
                     /locus_tag="IW728_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1023721.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15726..16751
                     /locus_tag="IW728_000338"
     CDS             15726..16751
                     /locus_tag="IW728_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1023722.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17346..20999)
                     /gene="rpoC"
                     /locus_tag="IW728_000339"
     CDS             complement(17346..20999)
                     /gene="rpoC"
                     /locus_tag="IW728_000339"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1023723.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21078..24695)
                     /gene="rpoB"
                     /locus_tag="IW728_000340"
     CDS             complement(21078..24695)
                     /gene="rpoB"
                     /locus_tag="IW728_000340"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1023724.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25423..26391
                     /locus_tag="IW728_000341"
     CDS             25423..26391
                     /locus_tag="IW728_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1023725.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26409..27314
                     /locus_tag="IW728_000342"
     CDS             26409..27314
                     /locus_tag="IW728_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1023726.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27311..28117
                     /locus_tag="IW728_000343"
     CDS             27311..28117
                     /locus_tag="IW728_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1023727.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28274..29242
                     /locus_tag="IW728_000344"
     CDS             28274..29242
                     /locus_tag="IW728_000344"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1023728.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29559..30083)
                     /locus_tag="IW728_000345"
     CDS             complement(29559..30083)
                     /locus_tag="IW728_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1023729.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30370..>31434
                     /gene="scm"
                     /locus_tag="IW728_000346"
     CDS             30370..>31434
                     /gene="scm"
                     /locus_tag="IW728_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1023730.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         8821 a         6786 c         5923 g         9904 t
ORIGIN      
        1 cggtcaagtt caacaacagt ttttacgcgt cgatctctgg cgctttgccc aaatgagctt
       61 ggatcatcca gatacgtttt tctgtggctg ttttgaattc gataaagata tcttgtgttg
      121 gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg gtaaagatct gctagataac
      181 gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt acgatcccag cggccaattt
      241 catcagggat ttttgtgtta tctgcaaatt cacgcaatgt agaataaggt gaaccgtcta
      301 atgtgatcaa gcgttcggat acttcatcta actgagcgtt caaatcatcc atgaactcat
      361 ccatcattgg gtgcaatgta aggaaacctg gtcctctcat gtaccaatgt gtttggtgaa
      421 caacaacaga catttggctt agatctgcaa ctaattgatt taatacttct ttggtttgtt
      481 ggaatttcat ctttcttgcc tcctaacatt ttcttacact taaaataata gtagactttc
      541 aatgaaaagt acaacaaaat catttacctg agaacgattt tcagttcgct tttttatccg
      601 taaagccaat catttttcag aatcagctaa ttcttctcgc agttgagcaa attgctgata
      661 agtctgtgct aaatgctttt gtgtcgaaac aaataatgga tagattttct gatatttttt
      721 atgatcagtt tcttgtactc caacttgctc tttcaattct atcaattcct gtgcttcact
      781 gatactttgg atctttccga tgcttctcca gcctaaaatc acagctccta atgcggatgc
      841 ttctgtgaca gtagggaaaa caagcgggta acccagtaca tcagccatca tctgacgaaa
      901 aatcggagat tgagcaaacc cgcctgtagc acggatctct gtgagctgat tttcctggca
      961 aactcctttt aaaatccgat aaaggttgag acagatccct tctagaatcg ccttcatcat
     1021 ttctttttct gtatgttgcc gctgtaaacc gataaaactg ccaaacgcat ctgcgtccca
     1081 aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc agaccattgg cacccgctgg
     1141 cacaaaagcg atagccgaaa gcagtgcatc atatggattt tctccttttt ctttagcaga
     1201 ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca aagatgccag caccattgct
     1261 agtcgctcct cccactaccc aatgtttctg atccagaaca taacaaaacg tctccatttc
     1321 tggatggaac tgaggctgag tagtaataaa acgaagtgct ccgctcgttc ctacggtcaa
     1381 agcggcgatc gtatcaccaa aagcacctaa ccctagatta gaaagcggcc cgtctgcccc
     1441 tcccaaaaca aatgatatat catttggcaa acctagttct gctgctgttt ttgatggtag
     1501 acctgttact tgataggtgg aaggaaagac ttgcggcaat tgctctattc gaatgttcaa
     1561 gtagtcaagc acttcttctg cccattggaa ttcatggata tcaaaatatc cagttcctga
     1621 agctgttccg tagtcgctga tcagttcgcc aaacaatcta taaaagatat agtctttgat
     1681 accgacaaaa taagcagttt ttttgaataa ttcaggttgg ctgttttgca gccacttgat
     1741 tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc tgccgataaa attctttgcc
     1801 aagcacggta tatttcagtt tttctgcttc gtccgctgct cggttatctg cccaagtgat
     1861 cattcgagat aacggtgttt tctcttcatc caacaatatc aggccatgca tcgcagagga
     1921 gaaggagaca aattgaatga ttgtgtctga aggcaactgt ttaattactt cttttataac
     1981 ggtgcagaca ccttgaaaga tcaattcctg atcttgttct gccatccctt gtgtttcttg
     2041 gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg gcttcatctg tgaaagcaac
     2101 tgctttggtt tgtgtggttc cgatatctaa tccgattgaa acgttcattt tttcacctca
     2161 aattttatgt atgtacaaaa atttagttta agcttgcgta tcttggtttt gaaaaggggg
     2221 agatggcgtg ttactttatt ttatcattgg ttgctgtatc ggatcatttt tatgtttaac
     2281 cgcccagcgg attccgttag gtcactctat tatttatcct agatctcatt gtgtcaaatg
     2341 ctgtcgttcg ctttcttggt atgaattgat tccgattctc tcgatcatcg tccaacgatt
     2401 tcgctgccgc tattgcaggt gtcggttgcc cttctactat ttgttggcag aagccttatg
     2461 tggtggtctt tttgcctggt tctttacttt ttcttccgca agaaattttt cgacgttcat
     2521 atggatgctt tcggctttgc tattttcgtt gatggattta ttttacttca tggtccattc
     2581 tcacacgcta ttctgttctt gggcaatttt atggattttt tggttgcagg ccggcgtctt
     2641 ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt gtgtgccttc gctatggtca
     2701 atcctatctc ggagctggcg atactctgtt gatgctttct tggagtggtg gcttgtctct
     2761 agaagagttg cttcagatct tgtttcttgc tagtctgttt ggaatatccg ttttttctgt
     2821 ttactttatc tgtcataaaa aaaagttgga aaagctgcct ttcattcctt ttttgagcag
     2881 tgcgttgctg attgtccttc atcttaggta ttgaaaaaag gttgtgacaa acgacttgtc
     2941 acaacctctg atatatagtg ttctcttacc aaatccttgt gacttggagc taggcgacag
     3001 gttcacaacc tctccactaa atggtgttca atcagctgac cctcagtatt agctcaaaat
     3061 gctggtttta caacctctga tatacggtgt tccataagta gctttttcgg cttcttattt
     3121 cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca gaaccttctc gataaagatt
     3181 gtaatggagc ttgtttttct tgtaataatc ctgatggtaa tcttctgcag gataaaattc
     3241 gcttcttggt tcgatttttg tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc
     3301 ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca ttatagtaga tcactggacg
     3361 ataagaatca ccgcgatcag cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca
     3421 atagatttcc accaactgat cataagaaat aacttctgga tcatattcga tctctactgc
     3481 ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa gttggatttg gtacatgccc
     3541 gccagtataa ccggatgtca cagaaatgat ccctggctgc tcttcaaatg gtttgaccat
     3601 acaccaaaag catccgcctg cgaaaactgc tttttttgtc atataaaatc cccctttttt
     3661 ctcatcatac cttgatttcc aaaaggagga taacgttttg cttgtgacag aaaaaacgga
     3721 aactattata tacgtgatac ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa
     3781 acggttaaat caggaatcac atattcagca gaaggaagat ttatgttttc acctattccc
     3841 aatacatgca tgttaccagc caaagctgct tgacaaccag cttctgaatc ttcaatgacc
     3901 aagcatgcat ttggcggtac attcaattgc tgtgctcctt tgagaaaaac ttctggatca
     3961 ggttttgctt ttgatacttg cgtgccgtca ataaggacat caaaataact tgtcaaattc
     4021 gttttttcta aaatgaggcg tgcatttttg cttgctgatc ccaatccgat cttgatgttt
     4081 gtttttttca agtactcaag aacagctatt gcaccaggca aaacggatgt ttcatccatt
     4141 ttttgaatcg cttgaagata tagattgttt ttttgctcag caaaggctga tttctctttt
     4201 tctgagaatt tctgttccat ttttccaaat gacagcaatc gctctagtga ctcggttctg
     4261 gatattcctt tcagttgctc gttttctttc tcagtgaatg gaatggacag ctggttagct
     4321 aaaaccagcc acgcttcgta atgataacga gctgtgtcta caagaacgcc atctaaatcg
     4381 aatagtgctg ctttgatttt catcgattac ctccttgaaa atgaacgact tgaagtaaag
     4441 atgcgatgtt tttagctgtg cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta
     4501 aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat gccaacatct gctgcttttt
     4561 cgcttccttt accaacactt ccgcaaatag ctatcacagg aacgccctct ggtactcttc
     4621 tagcaatccc aactggcgct tttcctttta gactttgtcc atctagacgt ccttcgccta
     4681 cgattacgag gtctgcttgc tgtacccgtt tatcgaactc cagtaaatcc aatacgtagt
     4741 cgatgccaga gactattttc cctgaagcaa atgctaacag tcctgcaccc attccaccac
     4801 cagcactact gcccgatgcc tcgattactt gaggggcaaa tctcatataa aaatcatgca
     4861 tggcttggtc agccactgct aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa
     4921 aagaagcgcc ttcttttccg cataaaagat tagtaacatc agtaatgaca gtgattttgg
     4981 ctttaatcag ctcaataggc acgttcgttt tatcaactgt agcaatttta cctagatttt
     5041 ctcccagtgc ttctactttt tgattatctt gatcgtaaaa agcataacct agaccttctg
     5101 ccatgccgat gccgccatcg ttggtactac ttccgccaac gccgatgatg atgtgtcgaa
     5161 caccttgttg caacaattgt cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg
     5221 gctgttgttg gtttagtggg acatctttca atccgacgat ttctgccatt tcaaaaatcg
     5281 cagtttgacc gtctgaagcg tactggattt ctttgttttt gccaaatgca ttagtaacga
     5341 ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc
     5401 catcgcctat aggtagcaga tcatattcag cctgaacaaa aacttcttga aaacctgatt
     5461 gaatggcttg tgctgcctct aaggctgtca gactttcttt gaaagaatct ggcgcaatca
     5521 gaatacgcat tattttcccc tccgttccaa gatcagttct ttttccagct ggtattggtc
     5581 tccattgaca aaaataggta atgtttcttt cgtctctttt tttattacta tttgtttttt
     5641 tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca
     5701 tgctgaagga agtcttggag aaatagaaag agttccattc gttactgtaa tacctgaaaa
     5761 accaaatata actgctagcc atagtgctcc taatgaagca gcatgaattc cgtcatctga
     5821 tgaatgcgga tctggtccta aatcaatcag gcacgcttct tggaataaat gatacgccca
     5881 ttccatttct tggatacggc aagcctctat ggcgtgaatc gctttactca acgatgagtc
     5941 gtggattgtt ttgttttcat agtaatgcaa attcttagaa acgattggtt gagaaaaata
     6001 ctcaggtaat aagtataaca gcatgacaag atctgcttgc ttgctgactt ggagttcatt
     6061 tacttcgcta cgggcataat caagtaaaat agactggctg ccaggacgtt ctttgtaagt
     6121 ggtcaaatca ataactggct tactgagata cgtgtcatct tgcggaatca gctgttcttt
     6181 gttaggcact ggcaaatata acttatctaa aaaattttga cattctttca aaaaaagcgg
     6241 atcgccagct tgatatttaa cagctaaggc cacattataa tgcgccagat agttcgtata
     6301 tgcattattg tcgatatatt ctgtgtattc atctggacca atgactctgt gtatctctaa
     6361 atgatcatcg acttttacag ctctgctcag ccaaaaagct gctgtttcat gaatgagcgt
     6421 ttttcccttt gtttctaaaa acgattgatc ctctgttgct tggacatact cttgaacggc
     6481 ataagcaagg tctgcactga tatgatgttc tgcggcagct gaagcaactt cttgtcttgt
     6541 tccggttttg atattcaatg cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct
     6601 ttcccatggg aaaagtgcac cttgatagtg ataatctctt gctttttgcc gtgcgccttc
     6661 taatcgatca taacggtaca gcagcaaatt ctttgctact tcaggaaact gatataaaaa
     6721 gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc cctttataac cttcgccagt
     6781 tagtcccttc gcccctacac taacacttgg atcattgcgg ggagtcataa tctgtaaatg
     6841 gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa tcgccttgga tcgttatccg
     6901 atgctgatgc caaaaatctg tccaataaga catcgactgc tctaaaattt ctgtataact
     6961 aacgcttttc tgggcttggg caatactttt cttaaacggg tctgtttgct gaagagaggt
     7021 agagagcacg atgatcttct ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa
     7081 ggaagcggtg atccggcgat tttttgcttg gaatacacct gctttattga atttagctgc
     7141 aatcccaata tcaattttac tttctgttgt ctggtaggat gcactgatca aatcgtcacc
     7201 aaatactcgc agttcttttt caaccaactg ctgtgtacca aaattggttt gctgtccatc
     7261 gatacccgtt ttcacatcta cttggcagtc ttcggtcaac ggcttgatga ccactttcag
     7321 ggctgcctga tggagattat tttgatcagc aacacgacaa gtaatgactt ccactttttt
     7381 tccttgagca gtttcaatga gcgcatagcg tttaagctct cccatttcta aatctagtgc
     7441 ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat
     7501 ttcccatctg atgacatctg ggagattcac taactcggct gattctgctc ctaaaggtcg
     7561 gttatataca ccagctgcaa acattcccct tacttgattc cgatagtctt cctcctggct
     7621 tcctctgata ccaaaaaatc catttccaat ggtcatctga ctcgcatact tattgatata
     7681 ctctaaatca aactgttttt cttctacttt cataaggtaa taacttctcc tgtttctgcc
     7741 gaacgatata aattttccac cagattctgg atgacatatc cttgccagcc atctgcaatc
     7801 atctgagtct ggtcaccgag cgcccgattc acaaaatgag ccatacttag ttcatgtggg
     7861 tcattaactg gcgtggtctt ttgtaaggaa accagctttc catctacatc ttcgaatagt
     7921 tcgcctggat acagattagc acctgcctga tcgccacaaa aagccacatt cagtttgtct
     7981 tctctgatat tcaaagcaaa cgaagtattc agttggatga tcttgccgtt ttctaattcc
     8041 agtgtgccaa aaattgcatc ttctactgtg aatttgcttg gatcccattt cccgaatttc
     8101 cctgaatttt ttttcgtacc aattttttgg aagctgtacg cccgaacgct ttttacttta
     8161 ggaaatccag taatatacat cgctgtatca agcatgtgaa tgccataatc gatcaaaggc
     8221 ccgcctcctt gaagttcttt gtcgataaac gttccccagc ctggaacacc agaccgacga
     8281 agcgcattcg cttctacgta ataaatatcc ccgagaacat caagatgatt cttgatcatt
     8341 gttgcttcgt cagaaaaacg atgctggaat ccataagcca aaatctgccc gtttttatcg
     8401 gctgtctctg ccatctttct tgcttgatca gctgtcatag ctggtggttt ctcgcacatc
     8461 actgaagcat tagcttcgag ggcagccaat acatgttcga agtgaaagcg attgggtgta
     8521 cagacactca cgatatcggg agaaatctca ttcagcattt gtgtaactga tgtatacgca
     8581 tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg atcaaccaca
     8641 gcggccaatt ctaagacatc tgatcttttt tgataataag cagcatgtac tttttctgct
     8701 acttgtccag cgcctatgat tgctactttc ttcatttatt tctctctcct ttgcttattt
     8761 acttattgtt attctttctc tattctttct ctaaatcatt taaatgacgc gctgtttcca
     8821 gacagttttt caagtattgc aatgattcct gataaatttt cacttcattt tctcctcgca
     8881 aacgacattc atataccata taaccttcat actcattttg aagtagcgtt tgaaaatggc
     8941 gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg attatctgct aaatggatat
     9001 gacccacata ctctttttgc gtagtcaatg cttttgaaat atcatcttct tcaatattca
     9061 tatgataaaa atcagcgatg atctgcacat gtttcaattg attagagtca atgtaatgac
     9121 gcgcatccgc taacaaattg atcatatggt cttggtatcg gttcaaaggc tccaaataaa
     9181 taaccgtacc tgtttcttcg gctactttat ctaaataacg aagtgattcg ctgactgctt
     9241 tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg taatcgataa gtgaacattc
     9301 cccaagctgc tgggataacg attcctttgc ctccaacctc gcttagtgca cgcaaaatct
     9361 cagcaatttg acgtacacca ttaagacgcc gttcttcgat aaagtcaccg atccacccat
     9421 cgtagccgtt acaagctgtc actactggga tccctgtttc tttgattgcc tgtttgactg
     9481 tgtcgatatt ttctattaac aacttaccgt cgatttcgta ttcttcaaat ccacagtctt
     9541 tgataaatct aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga
     9601 atttcattgt tctcctcctc ggacttgaac gcccattttg atactttctt ctgggtgctg
     9661 gtctacataa cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg gatcaatgat
     9721 ttcttcacaa tccaagtaac cgttcatcaa taattcccaa catgtgtcct cgattcgttt
     9781 acggttccat cttgggtaat ctgggtttgg ttcactagat gctcgagcaa agacgatttt
     9841 tgcattattg aaatgtgctt cgcgaccaag attaaaccca gctggaaaag gtttagcaaa
     9901 ggcaacataa gaaatgatcc cgccatagcc gatccctatt aaagaagctt gtaatgcggc
     9961 agcactgcca ctcgtttcaa taactgaatc tactccacgt ttatctgtca attcctttag
    10021 acgaaaaccg acatctgctt tcataggatc tagcgtgtaa tcggcaccgt gtttttctgc
    10081 taattctcta cgctgcgcta tgggatcgat cccaacgatg gtagtagcac ctgctttttt
    10141 agctaattga atcgcaattt gcccgatagc gcctaaaccg aagattgcta cagcatcacc
    10201 tgctcgaaca tgtgcttccc ggataccacc taaagcgaat tgagcaggat cgtaacatac
    10261 tgcatttttc cattgatcca accgatcaag tttacgtagt ttataattat caacaccgtt
    10321 tgtaatcagc gtttcacgaa tgggtccata agtgcagact aactctcctt tttgatactc
    10381 tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc atatttccta attgaaattc
    10441 gccaaatacc acaccgcgtt ggcttccttc ttctcttggc aagaataggc gccattcttc
    10501 tgaaaattct tcatcgataa atggacttaa tcctctaaag tcgacaattt ctgttcctgg
    10561 ttttggagta gcgaaaatta ctcggatacg tacttcgttc ttttcgatct cacggtcttc
    10621 gtaatctttg aatccggcta ttcttggttc taatgcaact aattttttca tcttttcttt
    10681 cctctttctt ttctttttat ccttttacgc cgccttcgac taaatcacct ttaataaatc
    10741 gttcagataa cgcatacata atgatgactg gtaaagcggt caccaatgaa gcggccatca
    10801 ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa
    10861 atttttcgga ggtcgacagg aaaatcgaag caaataaata atcattccac gaaaccataa
    10921 agcaataaac gaagacagaa atgattccag aaatagccaa tggtataatg atccgaataa
    10981 tgatctggat ccggctaaga ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg
    11041 tgtcaaaata actttttagc atgaatatcg cagtaggaag tgtttgaacg accatcgtga
    11101 taatcagtgc tgtattggta tcatacaaac ctagaccgga aatgatttta aataatggaa
    11161 caacgatcaa aataccggaa aacatataaa cggtgtaaaa gctagcattg attttcgagc
    11221 gagctttgaa gcgtagtttt gacaaagcgt atgccccgct gatcccaatc actactgcag
    11281 ttacggaggc tatcaaggag atcactaaac tatttttgaa atacatgatg aatggaaaaa
    11341 tctcagcatt gaaaatatcc tgataatgtt caaaagtcca ttctttcgga aaaatagttt
    11401 gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg
    11461 gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct
    11521 ttttattttt catttcaagt ccaccttctt tcgtgagagc aagacgaccg ctgcaataat
    11581 gatgaacaat agaatcgata tagctgctgc ttttccaaga tcattgaacg caaaggctgt
    11641 ctgatacaaa taaacgccta agatattgac tttattcgtc agtagataga catctgtaaa
    11701 catatagaac atccagatag atcgtaaaac aatcacagtt gctagtactg gtttgattgc
    11761 cggcagtgtc acgatcttga acttctgcca agcatttgca ccgtccattt cagctgcttc
    11821 ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg catacggata
    11881 gtatctccag atagcaaaca aaacgaccat aaagaagcta gcaactggct ggtcgaacca
    11941 cagcggtgct tcgttgaaaa gctgcaacct atctactaac agatcattga cgattccata
    12001 actattattg aacatatatt tccaagcaaa aatcaaagaa atagatggcg taacataact
    12061 taataaaatc agtgaacgca ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc
    12121 tacggctaat cctaaagctg tactgcctgc tacaactaag aatgtgtaag caactgttaa
    12181 acctaaagaa cgataaaatt cactatcact taaaatttca agataattgc ttaatccgat
    12241 aaaaacggcg tctgagttag gattcagcgg catatcgaaa aaactgatct gaatattgga
    12301 aagcattgga atcagcacta aaacagctaa taaaagcatg cttggtgcta aaagtcccat
    12361 ggctaatttt aagtcagaat gtttccttgg acgattcatt gaaacctccc cctgataatc
    12421 tgccaatagc tatctacttt tttcgaacac cattggcaga acttcttgtt atttcgtatt
    12481 tttttgtgcg tcagctaaag cttgatcgac atcttcgttt ccgacagtta cttggttcac
    12541 tgcttttccg atcacacctg agcttgtgat atctcccatt ttcgtgaaat tcttttcccc
    12601 gactagtcca aagacttgta cttcatcaaa agcactagca atctcagatg gcagctcgcc
    12661 aaaagacttg atgacttcat tttctttgta accatccagt tcgactactt gtttattcac
    12721 tggttgagca ccaccaggag acatcaagac ccacttttcc atgtttttcg gttgagataa
    12781 gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg tctagaccag aagaaatcgt
    12841 caatccagat actgtgccat agacagcttg atctttttgt gttgggatag caaaaccgat
    12901 attttctgag ttccccgctt cataaacaga aggcaaaatg taagttgaat agattgccat
    12961 tggtgctgtt ccattcatga aagcatcttt gatttctgtc acatcattag agcctggcat
    13021 cgtgtattgg gataagtttt gataaaacga caatgcttct ctcatctttt ccgtatcgat
    13081 cgttacctcg ccttcatcat ccaaaacatt tgctccattt gacaaagcaa attgagagaa
    13141 cgcttgctca ctcattgttc cttcagcagt aggcatagca attccatatt gtttattctc
    13201 tttatctgtg aaatgttgag caatttttaa aatatcatcc caattttctg gttctgaaaa
    13261 accagcttct gataattttt ctttgtcata ccaaatacct tgaacccagc cgctaattgg
    13321 tgcagcaata tagctttttc catcttctga acgaacaagg tttttggctc cgtcataata
    13381 attatcttct ccagcttcct cgataacgtt ttggactgcc gtctgatcaa tcagttcatc
    13441 tttatccatc actttagcaa aatcctgact gacttccatt acttctggta atttaccgga
    13501 acgagctaac gtaacgattt ttgtattgaa cgctgattct tctaccggca cttgcttgat
    13561 tttgatgtct ggattttctt tttcaaaatc agcaaccagt tcattgatca cacttaatcg
    13621 ttcctgttcg acagaagagt gcatgaattg aatcgttgtc tgttgtgatt cgttagagct
    13681 ttgactttca ccacaagcag taagaagaaa tccagtagct agcagacagg tagtaacaat
    13741 aatttttttc atttttttcc acctcttatt ttattatttt cagccacata tattcatacg
    13801 gctggaatat caactttcgt tcttgactgc aatggataat ttgtccagaa aacaggtcta
    13861 ggtaatctcc ttttggtaaa agataacctg ctgattgatt ggaaaaatta tggataacta
    13921 agactttctc ttccttcgct tcgcgaataa aactgataaa agaatctgct gagtccagta
    13981 agtaaatcaa actattcgga tggaaggctt tttctttttt tcgaatagat agcatctggg
    14041 tcaattcacc atatactgct gttcttaatg gatctgtctg caactctttc tcaatatctg
    14101 ctaacaaaaa tttttgccga ttgatttttc gattctctcc tgttttttct acgcctttgt
    14161 aatcatttcg acttcccagc acactttgaa tataaattgc cggcacgcct ggtaacgtta
    14221 ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag
    14281 aacttaacgc atctagatac gtagcgttta tctcatatgg actggtcgtt ccatctgggt
    14341 ttttcttgta gtttactcgg gcaccctctt tcttcagctg ttccactaat tcaaggatcg
    14401 ttttttccgg cagaatacct cgtaccggat tcaatccgat accgtcatgg gaggctaaaa
    14461 aattaaaaaa agttgttttt ttcgatgcga aatcgatggt ttttaaccat tctttcaact
    14521 tacttgtatt acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa aactgataga
    14581 ccatctgtgc ttcattctcg ccattcccaa aataactgat attatctttg tgtggcacat
    14641 ttgtctcagt gatcaatacc gttcctggag ctgcttcatc agcgattgca cggaataatt
    14701 ggatgatttt atgcgtttct ggcaggtgta tacaagaagt tcctagttct ttccataaaa
    14761 atcccacagc gtccaagcga ataaatgaag cgcctttttc taagtaaaac aaaagaatat
    14821 cgatcatttt caataaaact tctggatttt taaaattcaa gtctacttga tcttcgctaa
    14881 aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta agtaacgggc
    14941 tggtccttgg tctagtcacg ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat
    15001 ctttgtacgt atcattcaag tttaaatatt cctgaaacca agtactttcg gcagacatat
    15061 gattgcagac aaagtcgaac attaaccgtg tttcgcttct catttcttct atatcttccc
    15121 atgtgcccaa acggtcgttg attgcctgat aatttacaac ggagaaacca tcatcagatg
    15181 tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa tacttttgat
    15241 aaaatttctt aaatgtacgc aaagttggtt gctgctcttc aaaaaattga tccccataag
    15301 taatcaagaa aatatcttct tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct
    15361 gataaacact gactttttgt tcaatcatag aggcggtttt ctcagacaaa tctgagtcgc
    15421 gataaatcgc ttgtaaaagt tttgtataca tcggattccc ctttcttttt tgggagcgtt
    15481 cccaacgatt tgatcatatt atggaaaacg cttactgtca atagcatttt tttaaataat
    15541 tttgatatac acaaaaatac aaataagaaa gcttatgtat caataattaa tagcttctta
    15601 aaaaaatatg gtctctcatg atcatgcaag ctattcctaa attttcaaaa tcatagacaa
    15661 aaaagaatgt ctattgtact atggaattat tctaaaacgc tcccaaaaag gaggtgcata
    15721 taaaattgaa acctactatt tatgacattg cgaaaaaagc taatgtctca aaatcaactg
    15781 tctccagagt attaaataac cagccaaata tctctgataa agcaaaagca agtgtacttc
    15841 aagcaatcaa agaattgaat tatgaaccga gcaaaattgc aagaggactg tcaggaaatg
    15901 gttttgacgc gattctagca ttatctcatc gtacttctca tacgacctat ggcaatcctt
    15961 ttttttcaga tgttattcaa tccatttctg ccgtttctga acaaaataac ttcgacttga
    16021 ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa aagatccaag
    16081 aaaaaatgat ccgaggaatt attattttaa gctctacgat ggatgaaaca cttctttttg
    16141 aattggatca atacaatatt cctattgtgg taatcggaaa agtcggaaac gactacacga
    16201 acatctactc agtggacacg gataatttcc aggatagtta cgatttggtg gaaacactga
    16261 tcaagaatgg acaccgtcat atcggctgtc tccatgctcc gacagaagtt tatgtatcca
    16321 atgatcgtct caatggttac cgtcaatgct tatttgatca ccacctagat atccgtaatg
    16381 aatggattat agacggcggc tttcgcttag aagatagcat ggcagcaatg aatgatttaa
    16441 tgaagctacg agaaagacca actgcgattt tttctaccga tgcattaaaa tcattgagta
    16501 tttacaaagt ggcagaacaa tctggaaagc agatacctga tgatctttcg atcgcaggat
    16561 tcagtgatcc cttattgtcc tccctatttt cgccacagtt gactcggata gacattccga
    16621 caaatcagtt aggaatcaaa gccacagaac ttttattcca actaatccac caccaatacc
    16681 ctgaagaaaa aagaatcatt ctttcaaccg agattactct cacagattcg atccaacaac
    16741 aagaaatgta gaacagaatt ataaaagaac agaaacactg aggataagtc agttactctg
    16801 ttcttttttc aatcactcat ccctcgttac cccctcccgg tattttagcg aatttttctt
    16861 attttccatc tgttttagat agctgtggat aactataatg tttgtgttaa actatttttt
    16921 ccacagggaa gaaaaaacta tttttaacca cgttgtgtat aagtttttat agttcgtttt
    16981 tttaaacaca tttaccctta tttcaatcca tgttatttgg ggatacttat ctctaaaaca
    17041 taaatagaat aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata
    17101 gctagcaaaa tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa
    17161 aatgatctat ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct
    17221 taattggata cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa
    17281 ttattttaga caaaccaaaa aagaggttgt gacaataatt tgtcacaacc tcttttttaa
    17341 aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct
    17401 gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa
    17461 cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca
    17521 agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg
    17581 aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag
    17641 acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta
    17701 aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca
    17761 cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc
    17821 ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca
    17881 tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg
    17941 tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt
    18001 gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata
    18061 tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca
    18121 aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta
    18181 tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta
    18241 ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt
    18301 ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca
    18361 cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc
    18421 aattcatttg tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct
    18481 tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca
    18541 gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc
    18601 aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga
    18661 gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat
    18721 tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag
    18781 ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc
    18841 aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca
    18901 cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca
    18961 acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg
    19021 atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca
    19081 agcatcttag aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct
    19141 aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca
    19201 gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt
    19261 tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt
    19321 gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg
    19381 ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg
    19441 ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag
    19501 tagttaccta gaaccatatc ttgagaagga gtaacaactg gtttaccatc tttagggttc
    19561 aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat
    19621 ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt
    19681 ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt
    19741 cggtgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa
    19801 acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa
    19861 gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt
    19921 tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga
    19981 cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc
    20041 aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga
    20101 cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg
    20161 atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca
    20221 cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt
    20281 gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt
    20341 cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt
    20401 ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta
    20461 tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt
    20521 tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt
    20581 cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc
    20641 atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca
    20701 ggagctgcta attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc
    20761 actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca
    20821 cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc gcgttcaggt
    20881 tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg
    20941 attttttcgg gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa
    21001 aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt
    21061 gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca
    21121 tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc
    21181 tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg
    21241 tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat
    21301 tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca
    21361 cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc
    21421 aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca
    21481 ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag
    21541 taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg
    21601 tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga
    21661 acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca
    21721 cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc
    21781 aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc
    21841 atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg
    21901 gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt
    21961 ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct
    22021 tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat
    22081 gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct
    22141 gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg
    22201 acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg
    22261 acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat
    22321 tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg
    22381 atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg
    22441 cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg
    22501 actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga
    22561 cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca
    22621 gaagcatcaa cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca
    22681 tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc
    22741 actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag
    22801 aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct
    22861 actttatcaa tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga
    22921 gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg
    22981 atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga
    23041 cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga caaactattg
    23101 atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag
    23161 tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag
    23221 gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt
    23281 gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc
    23341 aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt
    23401 tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata
    23461 cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag
    23521 aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga
    23581 tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg
    23641 acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa
    23701 tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt
    23761 cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga
    23821 ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct
    23881 aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct
    23941 gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt
    24001 gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg
    24061 ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt
    24121 aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc
    24181 atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt
    24241 ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact
    24301 cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag
    24361 aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt
    24421 aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct
    24481 ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa
    24541 atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga
    24601 atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg
    24661 cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat
    24721 tcaaaaagac tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca
    24781 acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa
    24841 ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt
    24901 cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct
    24961 ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt
    25021 caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta
    25081 tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc
    25141 accaacacaa ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga
    25201 tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag
    25261 caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta
    25321 tcaagcattc tactttttca atttattttc cgacttttca atccatttgg acattttgtc
    25381 tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc
    25441 atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc
    25501 gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg
    25561 ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc
    25621 gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg
    25681 agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca
    25741 caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg
    25801 gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca
    25861 gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa
    25921 acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca
    25981 gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat
    26041 gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact
    26101 gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca
    26161 cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc
    26221 gactacgaaa gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag
    26281 aatccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac
    26341 atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga
    26401 ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat
    26461 gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga
    26521 ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct
    26581 atcatccatt gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt
    26641 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
    26701 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
    26761 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
    26821 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
    26881 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
    26941 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
    27001 ctggctcctt acttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
    27061 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
    27121 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
    27181 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
    27241 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
    27301 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
    27361 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
    27421 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
    27481 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
    27541 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
    27601 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
    27661 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
    27721 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
    27781 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
    27841 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
    27901 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
    27961 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
    28021 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
    28081 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
    28141 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
    28201 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
    28261 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
    28321 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
    28381 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
    28441 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
    28501 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgccaaact
    28561 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
    28621 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
    28681 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
    28741 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
    28801 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
    28861 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
    28921 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
    28981 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
    29041 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
    29101 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
    29161 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
    29221 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
    29281 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
    29341 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
    29401 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
    29461 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
    29521 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
    29581 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
    29641 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
    29701 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
    29761 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
    29821 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
    29881 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
    29941 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
    30001 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
    30061 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
    30121 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
    30181 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
    30241 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
    30301 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
    30361 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
    30421 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
    30481 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
    30541 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
    30601 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
    30661 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
    30721 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
    30781 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
    30841 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
    30901 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
    30961 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
    31021 gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt
    31081 aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt
    31141 tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag
    31201 atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact
    31261 atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca
    31321 gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca
    31381 gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc atca
//
LOCUS       DACEMO010000010        31322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEMO010000010 DACEMO010000000
VERSION     DACEMO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.010"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            147..1016
                     /locus_tag="IW728_000347"
     CDS             147..1016
                     /locus_tag="IW728_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1023731.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IW728_000348"
     CDS             1225..2250
                     /locus_tag="IW728_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1023732.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IW728_000349"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IW728_000349"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1023733.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="IW728_000350"
     CDS             3333..5036
                     /locus_tag="IW728_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1023734.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IW728_000351"
     CDS             5038..7071
                     /locus_tag="IW728_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1023735.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IW728_000352"
     CDS             7082..7522
                     /locus_tag="IW728_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1023736.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IW728_000353"
     CDS             complement(7722..8849)
                     /locus_tag="IW728_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1023737.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IW728_000354"
     CDS             complement(8852..9529)
                     /locus_tag="IW728_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1023738.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="IW728_000355"
     CDS             9793..11559
                     /locus_tag="IW728_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1023739.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="IW728_000356"
     CDS             11574..12011
                     /locus_tag="IW728_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1023740.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="IW728_000357"
     CDS             12024..13175
                     /locus_tag="IW728_000357"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1023741.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="IW728_000358"
     CDS             complement(13237..13935)
                     /locus_tag="IW728_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1023742.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="IW728_000359"
     CDS             complement(13928..15280)
                     /locus_tag="IW728_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1023743.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="IW728_000360"
     CDS             complement(15503..16903)
                     /locus_tag="IW728_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1023744.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="IW728_000361"
     CDS             17064..17801
                     /locus_tag="IW728_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1023745.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="IW728_000362"
     CDS             17926..18405
                     /locus_tag="IW728_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1023746.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="IW728_000363"
     CDS             18402..19169
                     /locus_tag="IW728_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023747.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="IW728_000364"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="IW728_000364"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1023748.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="IW728_000365"
     CDS             complement(20072..20674)
                     /locus_tag="IW728_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1023749.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21249
                     /locus_tag="IW728_000366"
                     /pseudo
     CDS             20780..21249
                     /locus_tag="IW728_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21355..21939
                     /locus_tag="IW728_000367"
     CDS             21355..21939
                     /locus_tag="IW728_000367"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1023750.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="IW728_000368"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="IW728_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1023751.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="IW728_000369"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="IW728_000369"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1023752.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="IW728_000370"
     CDS             23840..24019
                     /locus_tag="IW728_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023753.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="IW728_000371"
     CDS             24041..25066
                     /locus_tag="IW728_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1023754.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25241..26485
                     /locus_tag="IW728_000372"
     CDS             25241..26485
                     /locus_tag="IW728_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1023755.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26638..27585
                     /locus_tag="IW728_000373"
     CDS             26638..27585
                     /locus_tag="IW728_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1023756.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27719..28348
                     /gene="upp"
                     /locus_tag="IW728_000374"
     CDS             27719..28348
                     /gene="upp"
                     /locus_tag="IW728_000374"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1023757.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28487..28729)
                     /locus_tag="IW728_000375"
     CDS             complement(28487..28729)
                     /locus_tag="IW728_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023758.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28850..28922
                     /locus_tag="IW728_000376"
     tRNA            28850..28922
                     /locus_tag="IW728_000376"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28883..28885,aa:Thr,seq:cgt)
     gene            complement(29041..29679)
                     /locus_tag="IW728_000377"
     CDS             complement(29041..29679)
                     /locus_tag="IW728_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1023759.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29802..30434)
                     /locus_tag="IW728_000378"
     CDS             complement(29802..30434)
                     /locus_tag="IW728_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1023760.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30785..31084
                     /locus_tag="IW728_000379"
     CDS             30785..31084
                     /locus_tag="IW728_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1023761.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10690 a         5459 c         6300 g         8873 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt
    25261 tgaccctgat ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga
    25321 gctgattgcc tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt
    25381 gacaaataaa tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt
    25441 cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc
    25501 gaacgtccag cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga
    25561 accaggggac acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc
    25621 accagtcaac ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac
    25681 agaagttatt gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat
    25741 cgtagcaggt gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc
    25801 agatgaagta ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc
    25861 aggacttcat cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac
    25921 attgcgtggc cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat
    25981 aaatagtgcg gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa
    26041 agcagtagca ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat
    26101 cgccaatgca aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc
    26161 aggggcgaca gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa
    26221 agaagcagag agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt
    26281 tgaaacacta agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac
    26341 tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca
    26401 agcaaaagat gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga
    26461 aaaatttccg ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa
    26521 tgttgggctt ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct
    26581 ctctagtata tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg
    26641 actgatcgta tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat
    26701 ccgcgtatct atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa
    26761 tcacctgtga acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg
    26821 ggaaaactca atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt
    26881 ttttatattc atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc
    26941 agagaacttg cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca
    27001 gaagcaaaat atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa
    27061 ttagccaagt caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt
    27121 ggtaacatgg ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc
    27181 aagcaattgc tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa
    27241 ttcggagaaa actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg
    27301 acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa
    27361 ctaaatgatc ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa
    27421 ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc
    27481 caaggaatca tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt
    27541 gcagcaatgg cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc
    27601 aggaaaactt cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa
    27661 tcacttgatt ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat
    27721 gggcaaattt caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga
    27781 caaaaactgc ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc
    27841 atatgaagta tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga
    27901 aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg
    27961 tattggaatg gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg
    28021 tctttatcga gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga
    28081 cattgatgca cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat
    28141 catggcaatc gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt
    28201 agcagcacct gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac
    28261 agcttctcta gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc
    28321 gggtgaccgt ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt
    28381 gtcacatcct tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc
    28441 gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta
    28501 gttctttgta gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg
    28561 cagtttcatc aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt
    28621 gcgacttgcc gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt
    28681 ccgatggtac gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct
    28741 ttcacatcaa ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc
    28801 ggactcttta caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc
    28861 tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg
    28921 cattaaaaga gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt
    28981 gtttaaagga aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat
    29041 ttaggagaat aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca
    29101 acttgtaatg atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc
    29161 ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta
    29221 atggataaat ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact
    29281 agaaaaaata gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa
    29341 aataataaat aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt
    29401 caaaatttgt tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata
    29461 tttggctata tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt
    29521 accaaaaaca agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat
    29581 ggatggcgca aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc
    29641 ttttttaagt tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca
    29701 gtttttgaag atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga
    29761 tatttgtatt gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt
    29821 aaaaattttc tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgc
    29881 tccgagcgta cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca
    29941 gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa
    30001 cagtctacaa aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt
    30061 gaaccagata aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa
    30121 ctttccatca atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg
    30181 ctttttgtca atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc
    30241 attctttgaa aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt
    30301 ggatggcaga ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca
    30361 aaatcttcgt tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact
    30421 ttaggaaaaa tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa
    30481 ccaacttaca ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg
    30541 ctatctcttg aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat
    30601 aaaaatctta gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc
    30661 gttgccataa cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag
    30721 atgtagcgta tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat
    30781 cgtgatgtct aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt
    30841 aaatccagag atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga
    30901 aaaaggtcag tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc
    30961 aatcagggga gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg
    31021 aaaatttgaa aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt
    31081 ataaaaacga gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc
    31141 taggttcatt tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt
    31201 tctttatatt gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg
    31261 ttaattaaaa gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt
    31321 ta
//
LOCUS       DACEMO010000011        30555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEMO010000011 DACEMO010000000
VERSION     DACEMO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.011"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            175..660
                     /locus_tag="IW728_000380"
     CDS             175..660
                     /locus_tag="IW728_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1023762.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            653..1336
                     /locus_tag="IW728_000381"
     CDS             653..1336
                     /locus_tag="IW728_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1023763.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1462..4107
                     /gene="polA"
                     /locus_tag="IW728_000382"
     CDS             1462..4107
                     /gene="polA"
                     /locus_tag="IW728_000382"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1023764.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4152..4988
                     /gene="mutM"
                     /locus_tag="IW728_000383"
     CDS             4152..4988
                     /gene="mutM"
                     /locus_tag="IW728_000383"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1023765.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            4952..5581
                     /locus_tag="IW728_000384"
     CDS             4952..5581
                     /locus_tag="IW728_000384"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1023766.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5633..5965
                     /locus_tag="IW728_000385"
     CDS             5633..5965
                     /locus_tag="IW728_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1023767.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6277..6768
                     /gene="nrdR"
                     /locus_tag="IW728_000386"
     CDS             6277..6768
                     /gene="nrdR"
                     /locus_tag="IW728_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1023768.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6791..8164
                     /locus_tag="IW728_000387"
     CDS             6791..8164
                     /locus_tag="IW728_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1023769.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8164..9090
                     /gene="dnaI"
                     /locus_tag="IW728_000388"
     CDS             8164..9090
                     /gene="dnaI"
                     /locus_tag="IW728_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1023770.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9219..9659
                     /locus_tag="IW728_000389"
     CDS             9219..9659
                     /locus_tag="IW728_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1023771.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            9803..10504
                     /locus_tag="IW728_000390"
     CDS             9803..10504
                     /locus_tag="IW728_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1023772.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            10501..11622
                     /locus_tag="IW728_000391"
     CDS             10501..11622
                     /locus_tag="IW728_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1023773.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            11637..12869
                     /gene="pepT"
                     /locus_tag="IW728_000392"
     CDS             11637..12869
                     /gene="pepT"
                     /locus_tag="IW728_000392"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1023774.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            13001..13909
                     /locus_tag="IW728_000393"
     CDS             13001..13909
                     /locus_tag="IW728_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1023775.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            13944..14216
                     /locus_tag="IW728_000394"
     CDS             13944..14216
                     /locus_tag="IW728_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1023776.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            14227..15273
                     /locus_tag="IW728_000395"
     CDS             14227..15273
                     /locus_tag="IW728_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1023777.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            15524..18133
                     /gene="clpB"
                     /locus_tag="IW728_000396"
     CDS             15524..18133
                     /gene="clpB"
                     /locus_tag="IW728_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1023778.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(18284..18955)
                     /locus_tag="IW728_000397"
     CDS             complement(18284..18955)
                     /locus_tag="IW728_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023779.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(18948..19442)
                     /locus_tag="IW728_000398"
     CDS             complement(18948..19442)
                     /locus_tag="IW728_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1023780.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(19462..20682)
                     /locus_tag="IW728_000399"
     CDS             complement(19462..20682)
                     /locus_tag="IW728_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1023781.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            20829..22664
                     /gene="lepA"
                     /locus_tag="IW728_000400"
     CDS             20829..22664
                     /gene="lepA"
                     /locus_tag="IW728_000400"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1023782.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            22861..23772
                     /locus_tag="IW728_000401"
     CDS             22861..23772
                     /locus_tag="IW728_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1023783.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEISKESNRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFNKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            24770..25021
                     /locus_tag="IW728_000402"
     CDS             24770..25021
                     /locus_tag="IW728_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023784.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFVESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            25104..25376
                     /locus_tag="IW728_000403"
     CDS             25104..25376
                     /locus_tag="IW728_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023785.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            25466..25705
                     /locus_tag="IW728_000404"
     CDS             25466..25705
                     /locus_tag="IW728_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023786.1"
                     /translation="MIDISIPEDLKPDPATIGNSGMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMIFGDGEVANKAGIPIGKWTVDFHEAGSFFVL"
     gene            25852..30147
                     /locus_tag="IW728_000405"
     CDS             25852..30147
                     /locus_tag="IW728_000405"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ1023787.1"
                     /translation="MYAKTKMNWLKALALVALSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKLNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KNMNGKVQAEIKDLDPGKYVLKEIKAPKGYALSDETIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKIANKDLVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKERYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVT
                     FTLYNGKGQAIGKYTTDQNGEILVKGLVANNYYFTENVPLESFQPNTGKQAFTITFRQ
                     NAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQEGKEITSILTFIADKANGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAVHVDINDDNQSVRVNNPTVHTTAVFEDGLTIADPTGLFTLNDTVEINE
                     ILPGKAYLVKGILMDKATGEALLVHGQPVESAVGFIAKAENETVIVPFTFDLTGLNGR
                     EMVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLIVGKTYTVNGVLMDKVTEKPVLVDGKEVSSTLTFVAETTDGTVNLDFILD
                     AHALRSKEVVVFEDLFREGVLLASHADITDKDQTIKIVSPTITTKATNANGGKEILAL
                     PNQKVVEWVQVNGLAIGQIYKLIVQGYLTPDGTPFEGTHAEKVFMADKEMMEFLFEFI
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQFVEAPKPPQPVK
                     PNEPQPVKEVKQAAMVPMLPKTGSEDNVLWIMIGMIFVLAAGLIGYDLYKVRD"
BASE COUNT        10440 a         5256 c         6499 g         8360 t
ORIGIN      
        1 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
       61 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
      121 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
      181 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
      241 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
      301 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
      361 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
      421 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
      481 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
      541 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
      601 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
      661 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
      721 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
      781 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
      841 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
      901 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
      961 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
     1021 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
     1081 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
     1141 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
     1201 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
     1261 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
     1321 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
     1381 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
     1441 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
     1501 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
     1561 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
     1621 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
     1681 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
     1741 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
     1801 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
     1861 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
     1921 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
     1981 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
     2041 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
     2101 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
     2161 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
     2221 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
     2281 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
     2341 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
     2401 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
     2461 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
     2521 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
     2581 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
     2641 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
     2701 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
     2761 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
     2821 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagataaa
     2881 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
     2941 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
     3001 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
     3061 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
     3121 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
     3181 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
     3241 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
     3301 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
     3361 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
     3421 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
     3481 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
     3541 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
     3601 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
     3661 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
     3721 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
     3781 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
     3841 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
     3901 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
     3961 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
     4021 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
     4081 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
     4141 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttagaacggt
     4201 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
     4261 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
     4321 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
     4381 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
     4441 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
     4501 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
     4561 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
     4621 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
     4681 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
     4741 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
     4801 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
     4861 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
     4921 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
     4981 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
     5041 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
     5101 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
     5161 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
     5221 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
     5281 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
     5341 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
     5401 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
     5461 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
     5521 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
     5581 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
     5641 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
     5701 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
     5761 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
     5821 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
     5881 cccagaaagt gaccacattt ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
     5941 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
     6001 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
     6061 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
     6121 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
     6181 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
     6241 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
     6301 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
     6361 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
     6421 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
     6481 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
     6541 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
     6601 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
     6661 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
     6721 gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg
     6781 aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag
     6841 aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact
     6901 aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc
     6961 tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc
     7021 gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa
     7081 agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa
     7141 agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg
     7201 attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa
     7261 aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca
     7321 agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga
     7381 agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc
     7441 aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga
     7501 tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc
     7561 tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa
     7621 agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa
     7681 cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc
     7741 aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct
     7801 tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt
     7861 gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc
     7921 acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca
     7981 agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg
     8041 acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga
     8101 gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat
     8161 ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa
     8221 aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc
     8281 gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa
     8341 ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat
     8401 gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca
     8461 ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca
     8521 aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg
     8581 agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa
     8641 ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg
     8701 agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt
     8761 gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt
     8821 tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac
     8881 gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca
     8941 tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa
     9001 gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa
     9061 atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc
     9121 tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc
     9181 gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta
     9241 tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag
     9301 gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga
     9361 gacaaagcat atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa
     9421 cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt
     9481 cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa
     9541 atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga
     9601 tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac
     9661 caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt
     9721 taaggagata tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata
     9781 attatctaga aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt
     9841 gggtgacttt gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt
     9901 acccgttgca ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca
     9961 aggtccttat gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac
    10021 cgtacgcctt gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac
    10081 gattgcgggt atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca
    10141 acgcttaagc ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg
    10201 atcttggctg caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa
    10261 aaaaatatat gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat
    10321 ggagctgatg ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg
    10381 gacaagagag cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca
    10441 agaggcacgt caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc
    10501 atgacaatca gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta
    10561 gctgaagaag gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga
    10621 gtaatgatga cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc
    10681 gatttgctga ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat
    10741 cagccacaag aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca
    10801 cacacgaaca tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt
    10861 attgaagtgg agagttattt agtaaagact catgagattc attataaaaa actggccgtt
    10921 tatgtacctg tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt
    10981 acacaaggtg attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg
    11041 gaagctggtg cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca
    11101 aaagttgagg ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca
    11161 gctcatcctt atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg
    11221 tttgggttag gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa
    11281 caagtaaaag aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa
    11341 tcctctgtga agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca
    11401 atcgcccaaa gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat
    11461 atgcaaagcg ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag
    11521 caacgattta tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt
    11581 ttcgtttctg aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt
    11641 acgaaaatct tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa
    11701 caagcaaaac gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag
    11761 aattagaaga gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg
    11821 gaacattacc aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg
    11881 atacagcaga ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg
    11941 agtctacgat cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa
    12001 atttgaaaaa ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag
    12061 cagatgacaa atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc
    12121 cttctatccc tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg
    12181 gagcggataa attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg
    12241 gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc
    12301 aagggaaaaa tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag
    12361 caatcgattt tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg
    12421 aaggtttctt ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata
    12481 tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc
    12541 aagaagagtt gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat
    12601 attacaacat gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag
    12661 caatgatcga gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt
    12721 caaaaatctc ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc
    12781 acggacgatt cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa
    12841 aaattgcaga attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt
    12901 tcgtcccaat gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt
    12961 gtcccgccct cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt
    13021 ttataacaaa agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta
    13081 tgtaaaagaa aagcagccag aattatatgt agaactagct gaaactggcc catcgattga
    13141 tgatgcggaa ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg
    13201 tggagacggc acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca
    13261 tgtcggatta cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct
    13321 caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg
    13381 tcaagtcaat gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc
    13441 agtttggatc agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt
    13501 ttttaagtta ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca
    13561 ttggaaagga aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac
    13621 aaattttgac gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc
    13681 cttagatatt ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac
    13741 gatccccggc attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc
    13801 tgtgggcact cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt
    13861 atcaaaagga ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt
    13921 aacagaaagt tggaagaaga gtaatggatg tattgttaca caatcttttg tggttttacc
    13981 ttttcattgt gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa
    14041 ctagcggctg gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt
    14101 tgggaggatt gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta
    14161 taggattcgc tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag
    14221 caggagatgt cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt
    14281 gtgatttttg ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa
    14341 ttgctgtcta ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa
    14401 atcgctgaag ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg
    14461 aaagatgggt tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca
    14521 ggaacgctga tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga
    14581 gcaggggtga cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta
    14641 gctggcttgg tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt
    14701 atcatcgctg gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc
    14761 agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt
    14821 ttgcggaaat gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa
    14881 accattatcc aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt
    14941 gtattttggt atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt
    15001 ctactgacca tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct
    15061 gctggtattg gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca
    15121 gttgacgcag tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc
    15181 tatggatttg tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa
    15241 gtaaaaaaag agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat
    15301 caaatcaaag aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat
    15361 atacatacaa tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa
    15421 accatgtgta tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca
    15481 aagaaataag tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat
    15541 gactacgacg cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca
    15601 tcaagatatc gacattgccc atgtatggaa aatcttttta caacctaatc attttggtcg
    15661 aaatttctat acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct
    15721 actggatgag tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca
    15781 gaatttcttt cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt
    15841 tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt
    15901 ttatttgaat aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag
    15961 aggaggagaa agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata
    16021 cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga
    16081 tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct
    16141 gataggagaa cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt
    16201 acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc
    16261 attgatcgct ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa
    16321 agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt
    16381 aggggcagga aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc
    16441 tcgcggtgaa ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga
    16501 aaaagacaaa gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga
    16561 agatacgatc agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa
    16621 catccatgac aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg
    16681 gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga
    16741 aatgaactct atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat
    16801 cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt
    16861 gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac
    16921 agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca
    16981 cgaattagaa gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg
    17041 tacgatacct caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga
    17101 gattaagatg gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt
    17161 aacgggtatc cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa
    17221 tgaaacactt cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc
    17281 ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt
    17341 cctaggacca actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt
    17401 tgactcagaa gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt
    17461 gtctcgtcta gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac
    17521 ggaagctgtc agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca
    17581 tcccgatgtc ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa
    17641 agggcgagta gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca
    17701 gttactgctg gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt
    17761 caacacgcta ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat
    17821 tttatttaca ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca
    17881 attggcacaa cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac
    17941 gtggattgct gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat
    18001 cactaaggaa gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa
    18061 atcaaaagta acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga
    18121 agaaatcgtc taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt
    18181 gtcagacctc cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc
    18241 cgaaatttct tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt
    18301 tttctaattt ataactttca aagtaacgct catctttttt actttgagac catgcaaaca
    18361 atcccattaa ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg
    18421 ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta
    18481 agaactggtc gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg
    18541 caaagccgaa taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca
    18601 agtaaccaat caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga
    18661 ttttgtccca aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga
    18721 tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt
    18781 tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg
    18841 tgattccttg agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa
    18901 cgaatacacc aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa
    18961 gtccctccat ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact
    19021 tttccgttca atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca
    19081 gctgtttctt gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca
    19141 gtatataagc cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc
    19201 ccggcatcga tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt
    19261 tctgtgtcag caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct
    19321 tcattttctt gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact
    19381 aactgttggt tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc
    19441 atctaaacat catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat
    19501 gatacgctct agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa
    19561 ataagaagca cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac
    19621 ggattttcct ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc
    19681 gaagacacca ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc
    19741 tgttccaaca aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa
    19801 atatggttcg atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt
    19861 tttataaata tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca
    19921 taagccattt cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc
    19981 agctacccag tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc
    20041 tgtgcgctca tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa
    20101 taaagcacca tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc
    20161 aactggtaat cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct
    20221 gattttaaag gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat
    20281 actgtaagga tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac
    20341 gataattaga tcttgcatca atccatggtt agcatattct ggataagttc caactccagt
    20401 cacaccaccc attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat
    20461 ttgagagatc agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg
    20521 ttttttggaa cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc
    20581 agtcacttgg aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac
    20641 cggttttaat tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat
    20701 tagcttgata acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa
    20761 aactctttaa attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag
    20821 gagacgctat ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga
    20881 tcatcgctca tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc
    20941 acactgtgac gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg
    21001 aacgtggaat cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag
    21061 aaacatatat ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct
    21121 ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg
    21181 aagcacaaac attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag
    21241 tcatcaataa aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag
    21301 acgtaatcgg aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga
    21361 ttgaagatat tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg
    21421 caccgctaaa agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa
    21481 atgtccggat cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg
    21541 gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact
    21601 ttttgatggt cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac
    21661 gagtagggga tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc
    21721 gaaaaatgaa tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg
    21781 atttgcggga agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag
    21841 aaacctctca agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg
    21901 atgtcgtcca agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat
    21961 ccgttattta ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag
    22021 attttcctga acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca
    22081 tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat
    22141 tcattacgat ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt
    22201 ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat
    22261 tggattatga aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga
    22321 atggagaaaa agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg
    22381 gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa
    22441 tccaagcagc tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa
    22501 acgtcttggc taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac
    22561 aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct
    22621 tcatggccgt tctgaaaatg gatgagcaag atagtgtgaa ataacttata atcaatcgaa
    22681 aaacctatta aatcaacctt tttgagtaga tgcaacactg aggattatct gtataaaaga
    22741 ttttaaactg tcatttggtt atcacggcga tactaaaatt aacaaaattg gaatattttt
    22801 tcttgaagat ttttagctgt cagtatatga tgaaagcatc tcataggagg tgcttttttc
    22861 atggttgata attcaagtgg aagggttctt aaatctctaa gaaaagagaa aaaactatct
    22921 caaaaaaagt tagctgattt agcaggtatt tcacaaagta ctttagttaa atatgaaaaa
    22981 gggtcaagaa aaatccctaa agacgtagat aatactctaa gcaagatttt gaatatagaa
    23041 actctaataa aagatgagga agataagatt gagattctca tcggtcaact aattgcatac
    23101 agagatatga ataagttact taacaaagag ttagctgata atataggaat tagtgaagta
    23161 ttactcagtt atgtgttaaa tagaaagaga aatccgtcaa aagaaatgca aaaaaaaatc
    23221 gatatttttc tattaagtaa tgaaaaagaa atttcgaagg agagtaatcg tgatagtgag
    23281 atatttagtt tatcaaaaga cgataaaatt gttatgggaa aaagaatacg agaagtccga
    23341 aagaaccggg aagagacgct tgaaaaattt ggtaaaaact ttaccatcta tacaggcaaa
    23401 aatgttataa gtagatggga aaaaggtatt aatattccag atattgaaaa actaatgaat
    23461 attgcctatc ttggtaaggt cactgttcct taccttatgt acggagaaga ttataagaat
    23521 atacttccaa aagatgaaag agttagcgat tttaacaaga taaattcttt tagtatggga
    23581 ttaaggatga ggaaaattag gaaagattat tatctagaaa gggaggagtt tggaaagctt
    23641 ttttctcctt caatttcaaa atggtccata gataggtatg agaatggtag agatataccc
    23701 aatactaatc gaattatcca atatgcttat ataggaaatc tttctttaga atttttgatt
    23761 tacgggatat aaaaatagtt tacgtcgata cttattaagt tatcgacgtt tttttattat
    23821 agaaaaattt ataaaaggag gtttcttgat atgataagaa acaaaaaggg tttgttgaaa
    23881 gttgctttac tggcagcttt tttctaagta actttttgac aagctcaatg gcaacacaag
    23941 cggaagaagt aatacaagaa ataactgtta tgaataccag ctagataaaa tgggctaggt
    24001 atgttatcca acaatttagt aatcactaga agcctagcga aagttcgctt gataagttat
    24061 agatgagtat tgatttgtag tctttccaaa tcaaaagggt acagagccac atgaaatatg
    24121 ttgtcctctg cagcgtcagg atgggaatcc ataccatgga aattcggaga tctgtgaggg
    24181 aatacttacg tggttaatat gtcggttgtg ttggacgtag taatggtcat agtgggtaga
    24241 ttgactgaat tcttatatga gaaaatgaaa ccaacaagaa gatagggtta tttatacaga
    24301 attggggatc ttttctagaa aagtaatcgg acaatgcgag attcgtacgg tcttttttaa
    24361 atttctctta aaaaaacggc atcgaatggg ttgggtgatt gataagcaat gaatcataga
    24421 ctgatataga ataaaaaaat gattctattt atctttttac atctatcgtt ttttcaagta
    24481 accgcaaatt gtatcagcat tcaacaattt ttgtatacaa gttctagtaa aaggatctga
    24541 agaaaaagtt ggttgttgtg aagataaaaa aagaatcagg ttttactgag tttttcttca
    24601 aaatttgtgt tattaggtta ggacaaagat aagaagaagt atgaagattt aaaaaaggaa
    24661 tgaggaaaag atagtaattt ccgattattg gagttttttt attttataaa gaagaagcga
    24721 acaagttgtg attcctagat ttcgcgcgtg attaattaat gagcaaaaaa tgcggaaagt
    24781 tttagtgttt aatacacgag gacaaagttt cttaggtatt gaaggcacac gtgtaacggt
    24841 tgatgggtgg tgtgattgtt tcgaggttgg aaaacatgtt gaacttatgg catttactga
    24901 gctaaaagat aagaacaacg tggaatttgt cgagagtgat attggttggg acgatcatga
    24961 agaagtttat ggaaaattta ttttgcggaa gggaaattct tatacgaatg gggaagcata
    25021 agtagcgatt tgttagagat cattgatagg attgaaattg tcgggaatat ttatgagaat
    25081 ctagagttat ttgaggtgat agaatgagtg atggttttga acaagtatca ataattaaaa
    25141 atcaagttcg ggagattctt agaaaaaaag gctatttggt agatagttat tttgaaggag
    25201 attacgaaac atgggtaggt gtctatgctc gtccagagaa taaacctacc tatttagatc
    25261 ctacgacgtc agaagatgcc tatttacaaa ataggtatcg agtagatggt tttaagcaag
    25321 actttgcgga gtggtttgaa tgggaaattg aaaacggaga agtaaaagaa gaataagcgt
    25381 acttgttgta tcaaaataag tcagtagaac aaccaaacca gtagctagta atgacaataa
    25441 tactggggaa tcggatcatt atggtgtgat tgatatttct attccagaag atcttaagcc
    25501 agatcctgca acaattggca acagtggcat gatttttgat tcgttagatg aagctatgtc
    25561 atatggttgg aaagaaatta aggatgaagg tagtaattgg tatggcatga tatttggtga
    25621 tggtgaagtt gccaataaag ctggtattcc aattgggaaa tggacagttg atttccacga
    25681 agcaggttct ttttttgttc tgtaattata gtgggagggt aaaatcattg atgaaatgtg
    25741 ttcagataag agcgccagat aaaaatagaa gagacagttc acgtcgttat cacttttggt
    25801 aacggtgttt tgttttatta tataaaattc tcaggaaaag gaggaattct tgtgtatgca
    25861 aaaacgaaaa tgaattggtt gaaagcactc gcattggtgg ccttatctca attctgtttc
    25921 ttggtgggaa ctgctcaagc acatgcagaa gaaacgacgg ctacacaacc tacggtggaa
    25981 gccattgccc aagtcgcttc tgaagtgaca gaagtccaac cagcagaagc cggggtccca
    26041 gctggtgaag ccactgttga acaggcccct gtgacagaag cgccaacggt taattccaaa
    26101 acgactctcg atcagacgat tggggaaaca caacacccag ggaatagtcc tgttacttca
    26161 caaccagttg aagaaactgc ctcaattaca acccctgctt cagaaacgtc cactccagaa
    26221 ggagaaaaag ctgccgaagc aacgccttct gtgaagccag agtcttctga gaaacaagcg
    26281 gaagaaaagc aacgaataga agaaacagtg gaacaaccag cggctactgc tgacaaagaa
    26341 ggaaaaaatg aggaaaacgt tgaaaaaaca atctcttcaa ccgcagctag tcaaaatcaa
    26401 ccaacgaaac taaacaaaga atctacggct agtattgaag atcaaaagaa aaaagagcag
    26461 gacattccaa aaacaggtac acctacactg ttagtaacag taccaaccaa tcgttcggtt
    26521 cgtgttcgcc gtgcaaccaa tacagctgtt ttaggtggtc ggaaaattga ttggggcgaa
    26581 aatattggtg ttacttctag tccgaacaat ccaaacttca ctttaaatta tatggttcgt
    26641 tttgagatta atggacaagt ggcgttttgt attgagcctg gtgtctcagc cgcagaaggt
    26701 caagtttata cagctactgc tttggaaaac tatttgaaaa attcaaatgt acggaaaaaa
    26761 acaagcttga tctcttattt aggatatgtc acaaataaag acaaatccga tgaacaatac
    26821 attgcggcac aatttatgat ttgggaagct ttgggcacaa aaatcccaat caaaaatatc
    26881 cgtgttgatt atgatcgtcg aaaagcagaa attcaacgtc tgcttaataa ttttgatgta
    26941 agagctactt ttcataatca aacacatacg ataaaaattg gcgaaacgtt aaaagtaaaa
    27001 agtggcaatg actttctaca acgtgtaaaa ggcattcaaa agccaaaagg gatcaatgta
    27061 tctattcatg ggaatgaagc atggatcaca gtcacaaaag aagcaccaga aaaggcaaaa
    27121 attcatttca atcagattga agtattgggt acgccattag cgtatcgtcg tccaggtgcc
    27181 caaacaattg gtatattgaa tccttttgaa cctggtgact ctgttgtcaa tttgactatt
    27241 ttgaaacagg gaaacattcg tgtctttaaa gaagatgcag aaaccggtgt gaaagcacaa
    27301 ggcgcagctt ctttagaaaa tgcggtgtat ggcttgtatc aagcagatgg gaaaaagatc
    27361 aaagaaatta ccttgaaaaa tatgaacggc aaagtacagg cagaaattaa agatcttgat
    27421 cctggcaaat atgttttaaa agaaatcaaa gcgccgaaag gctatgcact ttctgatgaa
    27481 acaattgaaa ttgttgttga accaggaaaa actgtcaatg cgaccgcaaa agatcaagtg
    27541 atcaaaggaa ccattgattt agtgaaaatt gctaataaag atttagtcga ttcaaccaat
    27601 ccagataaca aaccaaaatt agctggcatt gaaatctctt tgacgtcaaa aacaactggc
    27661 aaagtggtga aaaccgttgt gacagacaat gatggttacg ctacttttgg taaaaatact
    27721 gttgtctttg atacctacat tctttctgaa accaaaggaa aagaagggta taaactattt
    27781 gaaccacttg aagtcacaat ttctgaaaat gggcaaactt tccattatgt tttagaagat
    27841 aaagtaattg aacaacgtct taaagtggtg aaagtggatc aagaaacagg aaaagtgatt
    27901 ccacttgcca atacagaatt caagattttt gatactttag ccaatcgtta tgtcacaatg
    27961 gagattccaa atgatacaga aacaacggat atctttaaaa ccaatgaaaa aggctttttc
    28021 ctaactaacg gtacacttac ttatggaaaa gagcgctatc gtttggaaga aatcaaagca
    28081 cccgtaaatt acgttttgaa tcaaaaccca ttagtgttct cgattaccaa tgatacagaa
    28141 ccgatcaaag tgatccattt tgcgaaccgt caagcacgga aaagtgtaaa attgttcaag
    28201 tatgaagaat ggaacaagaa gaaaacacca cttgccggtg tgacgtttac gctatataac
    28261 ggaaaaggtc aagcaattgg taaatatacc accgatcaaa atggtgagat tttagtaaaa
    28321 ggtctggttg ccaataacta ttactttacc gaaaatgtac cattagaaag tttccaacca
    28381 aatacaggca aacaagcatt cactattaca ttcagacaaa atgcgccaga tgatggggaa
    28441 acgttattgg tttctgtacg taactatttg attccaccaa aattagaaac acaagcgtca
    28501 aataaagccg atggtgaaaa agtggttgat ccattagaaa ctatcgtcat taaagatagt
    28561 gtttcttata ccaacctgtt tgtcggcaaa gaatacacta tttcaggtgt attgatgaac
    28621 aaggcaacga atgcgccaat tttacaagaa ggcaaagaaa tcacaagtat cttgaccttt
    28681 attgcagaca aagccaatgg cacaaaagat cttgaatttg tggttaatgc tagtgttctg
    28741 aaaggtcaaa caatcgttgt ctttgaaaaa cttttccgtg atggcattga agttgcggta
    28801 catgtggata tcaatgatga caatcaaagt gttcgggtaa acaatccaac ggttcatacc
    28861 acagctgtat ttgaagacgg gttgacgatc gcagacccaa cggggctgtt caccttaaat
    28921 gataccgttg aaattaatga gatccttcca ggaaaagctt acttggtcaa aggaatctta
    28981 atggataaag caactggtga agccttactt gttcatggtc aaccggtaga aagtgctgtt
    29041 ggctttattg ccaaagcaga aaatgaaacg gtgatcgtac catttacctt tgatttgaca
    29101 ggtttaaatg gtcgtgaaat ggtcgtgttt gaaagtcttt ttagaaatga cgaagaacta
    29161 gcaaaccata aagacatcaa cgacaaaggt caaacggtac gagtaacgaa cccaagtgta
    29221 cgtacaacgg ctacacataa acccactggc ttgaaagagg ttaatccatt aacgaaagtc
    29281 accattacag acgctgtgaa ataccacgat ttgatcgttg gaaaaacata cacagtcaac
    29341 ggtgttctaa tggacaaagt aacggaaaaa ccagtgctag tagatggaaa agaagtctca
    29401 agtaccttaa cgtttgtggc agaaacaact gatggtacag tcaatctaga ctttatccta
    29461 gacgcacatg cgttgcgaag caaagaagtc gtagtttttg aagatctgtt tagagaaggc
    29521 gtattattgg cttctcacgc agatattacc gacaaagacc aaaccatcaa aatcgtcagt
    29581 ccaaccatta cgacaaaagc taccaatgca aatggtggaa aagagatctt agctttacca
    29641 aatcaaaagg ttgtcgaatg ggttcaagtt aatgggttag caatcggtca aatttacaaa
    29701 ctgattgttc aaggctactt aacaccagac ggcaccccat ttgaaggaac acatgcggaa
    29761 aaagtcttta tggctgataa agaaatgatg gaattcttgt ttgaattcat cgttgacggt
    29821 cgcaaccttg caggaaaagg attgagtttt gcggaatggc tccaagccaa aacagaagat
    29881 gaaaaagaaa cgtttgaaga agtcgcaaaa cataacgtcg atctaacaaa caaagatcaa
    29941 acggttcaat ttgtggaggc accaaaacca ccgcaaccag taaaaccaaa cgaaccacaa
    30001 cctgtaaaag aagtgaaaca agctgcaatg gtacctatgt tgccaaaaac aggctctgaa
    30061 gacaatgtat tatggatcat gattggtatg atctttgtcc ttgcggcagg attgattggt
    30121 tatgatttgt ataaagtcag agactaaaga ggacgaacaa gcggttaggc aactagccgc
    30181 ttgtttagtt tttagaaaga attttttatc aaaggcaaat aaattattgt gttgagtagt
    30241 aaatgatcat gaggatacac caagaaatac tgagtgtaat aaataagaat ttaaggatcc
    30301 aaaagagata gaatataatt atgtgataat acagtttctt tttttctata gaagaaatca
    30361 cgtttagcta ctcctaattg ctttcttact ttatttagtt ctgctaggta gcagtatgag
    30421 aattcaaaat acattctttg tactttgaag aggatttttt tcgaatgttt atttgtgtta
    30481 attatttttt gtatttcttt ggcaaaataa ataagttcat aaggcaaata aaagtctata
    30541 tttttatttt caact
//
LOCUS       DACEMO010000012        30395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEMO010000012 DACEMO010000000
VERSION     DACEMO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.012"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(101..2176)
                     /locus_tag="IW728_000406"
     CDS             complement(101..2176)
                     /locus_tag="IW728_000406"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1023788.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDYEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTYHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2178..3095)
                     /gene="glyQ"
                     /locus_tag="IW728_000407"
     CDS             complement(2178..3095)
                     /gene="glyQ"
                     /locus_tag="IW728_000407"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1023789.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3481..4293)
                     /gene="recO"
                     /locus_tag="IW728_000408"
     CDS             complement(3481..4293)
                     /gene="recO"
                     /locus_tag="IW728_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1023790.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            complement(4436..5335)
                     /locus_tag="IW728_000409"
     CDS             complement(4436..5335)
                     /locus_tag="IW728_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1023791.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5350..5751)
                     /locus_tag="IW728_000410"
     CDS             complement(5350..5751)
                     /locus_tag="IW728_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1023792.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            complement(5729..6205)
                     /gene="ybeY"
                     /locus_tag="IW728_000411"
     CDS             complement(5729..6205)
                     /gene="ybeY"
                     /locus_tag="IW728_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1023793.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6234..8426)
                     /locus_tag="IW728_000412"
     CDS             complement(6234..8426)
                     /locus_tag="IW728_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1023794.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8450..9421)
                     /locus_tag="IW728_000413"
     CDS             complement(8450..9421)
                     /locus_tag="IW728_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1023795.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10127..11506
                     /gene="brnQ"
                     /locus_tag="IW728_000414"
     CDS             10127..11506
                     /gene="brnQ"
                     /locus_tag="IW728_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1023796.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11662..12108)
                     /locus_tag="IW728_000415"
     CDS             complement(11662..12108)
                     /locus_tag="IW728_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1023797.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12135..12311)
                     /locus_tag="IW728_000416"
     CDS             complement(12135..12311)
                     /locus_tag="IW728_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1023798.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12473..12901)
                     /locus_tag="IW728_000417"
     CDS             complement(12473..12901)
                     /locus_tag="IW728_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1023799.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12993..13853
                     /locus_tag="IW728_000418"
     CDS             12993..13853
                     /locus_tag="IW728_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1023800.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13948..14241)
                     /locus_tag="IW728_000419"
     CDS             complement(13948..14241)
                     /locus_tag="IW728_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1023801.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(14314..16041)
                     /locus_tag="IW728_000420"
     CDS             complement(14314..16041)
                     /locus_tag="IW728_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1023802.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            complement(16255..17181)
                     /gene="trxB"
                     /locus_tag="IW728_000421"
     CDS             complement(16255..17181)
                     /gene="trxB"
                     /locus_tag="IW728_000421"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1023803.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17326..18759)
                     /gene="ascB"
                     /locus_tag="IW728_000422"
     CDS             complement(17326..18759)
                     /gene="ascB"
                     /locus_tag="IW728_000422"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1023804.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18759..20162)
                     /locus_tag="IW728_000423"
     CDS             complement(18759..20162)
                     /locus_tag="IW728_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1023805.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20392..21294
                     /locus_tag="IW728_000424"
     CDS             20392..21294
                     /locus_tag="IW728_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1023806.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21335..23221)
                     /locus_tag="IW728_000425"
     CDS             complement(21335..23221)
                     /locus_tag="IW728_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1023807.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23317..24168)
                     /locus_tag="IW728_000426"
     CDS             complement(23317..24168)
                     /locus_tag="IW728_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1023808.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(24625..26409)
                     /locus_tag="IW728_000427"
     CDS             complement(24625..26409)
                     /locus_tag="IW728_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1023809.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26409..28160)
                     /locus_tag="IW728_000428"
     CDS             complement(26409..28160)
                     /locus_tag="IW728_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1023810.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(28302..28526)
                     /locus_tag="IW728_000429"
     CDS             complement(28302..28526)
                     /locus_tag="IW728_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1023811.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28777..30363
                     /locus_tag="IW728_000430"
     CDS             28777..30363
                     /locus_tag="IW728_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1023812.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8432 a         6560 c         5432 g         9971 t
ORIGIN      
        1 aaaaaaatta acattgaatt aataatagtt atttataaca aataaaccag agaaatagcc
       61 tattttgtgc ctgttctctg gtttatttac atattagaaa ttattttgtg atgatttgat
      121 ctaaacttgc tagtgacaaa gccatggcag caatttgact taattgcgtt aatcggttct
      181 tacgtactgc tggttcttct gccatgacca tcgtctgatc aaaataattt tcgatcagtg
      241 ggcgaaggct tacaagctga tcataatttt cgttgatcgt ttggtcagca aagcttgttt
      301 tcattgcctc tactgctttg tgcagttctt tctccgcttc gttttcgaac agttcagggt
      361 ctacttctga ttgtttttca agctccgctt ttttcgctaa gttcacgaca cgtgtcaaag
      421 cttccatcga tggtttgaag tcttcatctg caagatgctg cttgaacaat tgtgctgctg
      481 caaatacttt tgtcagatct ttttgttctg cattcaaaac agcttcgatg acatcatgac
      541 gaatattctt cgttgtcaat aattggcgca gacggccttt gataaagtcg atcacctgtt
      601 gttgtcccgc tgacaagcca atgccaaaac gatccgcatc tttactgata acttcactaa
      661 tcgtttcttg cagaacagat aaaggaaagg cccatttttt cgattcgatg attcgaacga
      721 caccatatgc ttgacggcgc aaagcatatg gatcgtttga gccacttggg atcatcccaa
      781 ctgcaaagaa agaaaagata ctatctaatt tatcagcaat cgctaaaaca gaacctactg
      841 tagtctcagg tagatctcct tcactggata taggcatgta atgttcacgg attgctgttg
      901 ctacggctgg tttctcgcct ttaagcagag catatttttc tcccatgatc ccttgtagtt
      961 cagggaattc tcctaccatg ttggttacta gatcaaattt atagatttca gcagcgcgtt
     1021 gcagatccat caattcttcc tcagatagtc ctacttgctc gcctatgatg cgggcgatga
     1081 cttgtacccg ttgcattttc tcgtagatgc taccgatttt ttcatggtaa gtaacagttt
     1141 ttaatttttc aacgtattgg tcgattgaag ctttcttgtc ttcattataa aagaattccg
     1201 catcttctaa acgagcagtc aatacttttt gattgcctcg aaccacattt tccaatttta
     1261 cccgatcacc attacgagca gaaataaaat gaggaagcag cataccttga tcattccgta
     1321 cctcgaaata acgttgatgt tctttcatag aagtgactag tacttcttca ggaacagaaa
     1381 gatatttttc ttcgtagtcg ccagaaaaga cagttggata ttcgactaaa ttgttgactt
     1441 cttctagtaa atcttcgtct agatcaataa cccagtgatt tttttctgct aattcgtttg
     1501 cttgtgtaac gatcatttct tgtctttttt gcggagacgc aatgacgaat tgttcattca
     1561 atttttcttc atattctttt gcatgcgcaa atgtcacatc ttcgcctaag aaacgatgac
     1621 ctcgagaggc tctgcctgtt ttgacatcca agatttcgaa tgggattact tcatcatcta
     1681 acaatgccac gatccagtga atagggcgga tatattcaaa atcgtattct gcccaatgca
     1741 tcgttacagg gaaagtcagg cttttaatga catctgctaa acctgtcaat acttctttgg
     1801 attccttacc atgagtgtat ttcgttacat agacatattc cacacctttt aattctctaa
     1861 aagtgatagc gtctgtcgtc agaccttgtc cgcggacaaa tccttccgct gctttcgtcc
     1921 agtttccatc tgcgtccaaa gcaatttttt tggctggtcc tttgacttct tcttcactgt
     1981 ccgcttgtcg ttctggaata tttgttactt tgattgctaa acggcgcggt gtggaaaatg
     2041 tttcgatcgt atcataagtc aattggtttt catctaaaaa ttttgctgtt ttttcttcta
     2101 attgtttcat gcttggtgtg acaacatggg caggcatttc ttccagcccg atttcaagta
     2161 ataagtcttt tgccatctta ttcagcctcc ttcacggttt ctggatttgc tgtgttttta
     2221 ttcaacaaag ggaatcctaa tttttcgcgt tctgccacaa agattttggc tacagcacga
     2281 gccatgttgc ggatacggga aagataaccg gcacgctctg ttacggatac tgcaccgcga
     2341 gcatctaata aattgaatgt atggctgcat ttcaaaatat aatcatacgc tggatgaaca
     2401 agattttcat cgatacagcg tttagcttct ttttcaaaac gatcaaagtt gcttagtagc
     2461 aagtcttgat cgcttacttc aaaagaatat ttggaatgtt catattctgg ttgtttgaag
     2521 atctcgccat atttcacgcc atcagcccat tctaaatcat agacactttc aacttcttgg
     2581 atataagaag caagacgttc tagtccataa gtgatctcag aagtgactgg tttacaaggc
     2641 agtccaccaa cttgctggaa gtacgtgaat tgtgtgattt ccattccgtc aagccatact
     2701 tcccaaccca caccggcaca gcccatggat gggttctccc agttgtcttc aacaaaacgg
     2761 atgtcgtgtt ctaatggatc gattcctaaa aggcgcaagc tttctaaata aagctcttgg
     2821 atattctcag gtgaaggttt catcacgact tggaattgat gatgctgata caaacggttt
     2881 gggttttcac cataacgtcc atcagctggt cggcgagatg gttctacgta agcagcattc
     2941 catggttctg gtccgattgc ccgtaaaaag gtatatgggc tcatcgtacc tgctcctttt
     3001 tctgtgtcat acgcttgcat cagcatgcag ccgtttgatg accaaaattt ttgtaatgtt
     3061 aaaatcatat cttggacggt caatttttta ctcatctgtt ttgccccttt caattttaaa
     3121 gcgttgctgt caaataagaa tacaggaaag aaaacgacaa aaaatcccta tgtctgatta
     3181 cagacatagg gacgaataat tcgcggttcc accctacttc cgaataaaga ttcggcagct
     3241 tcatttatcg tactcgcaag tgcctcttca aaaatcgttt gggtccggct cccactctcc
     3301 cgaactcgct aaaccacgtg ttttttactg atcttggtca atgtacgtat tcattttgca
     3361 gttttcttgt ttctataata gatcctctca ctgaacgatc tatttgatct acggttaata
     3421 atagacggaa ctccctgtgt ttgtcaaatg gttttgattt tttgttgtat gtttcattta
     3481 ctattcttct tttttaggta gatctttttc agacatgtct ttctctggcg tctttaatac
     3541 gtgtgcccaa gacttcattt gatcgatgaa ctttttgctt ttcaaatgaa taccgacata
     3601 ttcatcatac agctggtcga tgagttcacg gattgcttgt ttggtttctt ctttcagatg
     3661 gattccactg attttatcat aagaaatagc tgaaaacatc cgaacgaaat aaacagctcg
     3721 aggatctgca tgataacggt gatcgtccat cgaccaatgt ctttcgcata tcacaccacc
     3781 atatttggaa gaatagtcaa attttccttt tgtttcccca catacgacgc agtgtgtcca
     3841 gattggtgca attccgaagc gttgcagtat ttgaacttca aagatattcg tgatgatttc
     3901 tgcatcatct ccttgatcca tccgctgcag agcttgttgc gtaaattgga ataaatgcgg
     3961 gtcatattga tgatcttcga ttgctgcatc tactagattt agcaaatagg taccataacc
     4021 gctcaaaaag atatcttctt ggatgttccg aaacgcatgg acgtctttga cactgcttaa
     4081 aaacgacaaa ccttcacttc gaaaattacc gatatagact gcttcggtaa aggggagcaa
     4141 agccggagct aatggctgat tttgccgatg gattcctttg atataaaaca tcattttgcc
     4201 agaagattcc gtaaagattt tgactaattt atctttttct ttgtaattac ggctagataa
     4261 aatcaagcct ttcgattctg caagctggct catattctcc ctcctaaaaa ataaatctgg
     4321 aaaagcagat tctgccgatc cagattattt atgtttcgat cttccgccgt ttttctcaac
     4381 gtgcggattt ttatccagtt tatttctata cttaacctat aagaagttca gaatatcagt
     4441 attcatcttt tcggtagccg aagtcttgca gatagacttt tttgtctcgc cagtcttttt
     4501 ggactttgac ccaaagttct agaaatactt tgtctcctaa aagattttcg atatcctttc
     4561 ttgctttcgt tcctacttgc ttcagcattt ttccgccttt gccgataatg attccttttt
     4621 ggctatctct ttcgacgatg atcgtggcct ggatatggat tttatcattc tcgtttcgct
     4681 tcatcgaatc aacaacgact gcgacagaat gaggcacttc gtctcgtgtt aaaaacaaga
     4741 ctttctcacg gatcaattca gaaacgatga agtattcagg atgatcagtc acttgatcat
     4801 ctggaaaata ttgtgggccc tctggcattt cactgactaa tacatccatc aaacgttcga
     4861 cgttgtttcc ttctgtagca gaaattggaa tcacttgtgc aaagggcatc tgcgttgaat
     4921 aatcttcgat gattcccaac aactcgtctg gatggatttt atcgatttta ttgataatca
     4981 gataaacagg agaatgcacg ttttttagac gttcaataat aaaatcgtct ccccggccgc
     5041 gtttttgatc agcgctgatc ataaataaga ccgcgtccac ttctttcaac gcactgtaag
     5101 ctgcttctac cataaaatct cctaaacgat gctttggttt atggattcct ggtgtgtcaa
     5161 taaaaatcaa ctgagcttct ggtgtagtat atactccttg gattttattt ctagttgtct
     5221 gcgccttatc actcatgatg gcaatttttt gacccacgat tcgattcaat aatgtggatt
     5281 ttcctacatt cgggcggccg acgattgcga caaaccctga tttatgttct ggcatatacg
     5341 ttctcctttt taattaaaca acaaatgata tattttaggg acaaataaaa tcaacccaac
     5401 gatgacggca aaacaagcgg tcactaaaac tgctccagct gccatatctt tgattttttt
     5461 cccaatcgga tgaaaatgaa aatccgtaaa catgtctacg acattttcaa aaacagtatt
     5521 gatgatctct actatccata cgagaaaaac agctaaaagc agccataacc attctatccg
     5581 atcaagttgg aaaatcacgc ccataaacaa agcgaggatt ccgaacgcaa catgtttcct
     5641 catgttgcgt tcttcctcaa aaacagtttt gactccttgg acggcaaatt ccacggacat
     5701 caggaaatgt ttgtttttct ctacgttttt atcttttaag tccataggca tctaagatct
     5761 ctttctgtaa accaaacatt tctttctcat cctctggtgt catatggtca tacccgttga
     5821 tatgcaagaa tccatgaacc gctaaaaagc caagttcccg atcatacgaa tggccgtaag
     5881 cttctgcttg ctctgcagca cgttcagtag agatcatgat gtctcccagt acacgaggca
     5941 atggattctc tccatcttca aaaatcactg ggatttcgtc ttcaccctct tcttccaagg
     6001 caaaactgat gacgtctgtc ggtgcatctt ttccacggta atcacgattg attacttgga
     6061 tcgctgcgtt gtccatgaaa gtaacagaca tttctgtttc ttctggcaac tctaaatatg
     6121 atgctgcaaa ttgcaacaag tcgtcgattt ctttcatttt ctcttctgag acttggtttg
     6181 tttcatccat gaaggtaata tccattatac atcgcttctt tctattcttc ttgctattct
     6241 tcttgttctt cttttatctt ctcaacttcc gatttcattt taggatattt gatccgagaa
     6301 tggaaggtac tgcttaaacc gctgatcaat gatttttcta ctttacggat ttcttttaac
     6361 gtcaatccac tgtcatccaa ttgtccatcg gaaatacgct cttctataag attatggaca
     6421 aaactttcga ttttttcgct tgttggatgg tccattgccc gtactgccgc ttcacaactg
     6481 tcagctatat tgacgattcc tgcttcacgg ctttgaggtt ttggtccagg ataacggaat
     6541 tgctcttccg tgacttccgg gttgcgttcc tttgccttaa cgtagaaata acgcatcagc
     6601 gtggttccat gatgctgtct gcaaatatcg atcaccattt gcggcatatt ataatcttcc
     6661 aatattttgg caccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat
     6721 aagaaattgt gcggattttc cgctcctgaa ggcaagttct caacgaaaaa gctggcgtgt
     6781 ttgatcttgc cgatatcatg ataatagcaa gctactcgag ttagtaaaga acgtccaccg
     6841 atttctgcta cagcattagc acttagattg gctaccatca tgctatgatg atacgtacct
     6901 ggtgcttctt ctaacaactg tttcaataaa gggtgattag gattgctgag ttcatttaat
     6961 acgatcacac tatcatcatt gaccatcaat tcaatatagg ggtgcagacc tatcgtcagc
     7021 aagaatgaga acaatgcacc tgtgaatccg cagactagcg ttaaccaagt cgttgcatcc
     7081 ccaaagctca ttccttggta gataatcaaa atgacatcca tcatcacagg gaaaataatg
     7141 acccacatag cagcgaataa cccttgttct gagatccgtt tgcgtctgac gatcgttgcc
     7201 atgagaccag aaaatacata ggacatcaaa ataactgtca gaaagttcgt gccaatcgaa
     7261 tcataaaaaa tgaaaagtgc ggatactgct tggaaaatac cagccatgat ccctgctctt
     7321 cgattcaaga aaaagcttaa aacaagtgga gcaaaagccg cggggtaaaa cagcggaata
     7381 taagcagctt gttctgtttg gaagagttgg aaaaatttca ttagtactac gctgactgac
     7441 attgctgtca cgtagaaaag tacgtatttt gttctttctc ctgcattttg aaattgaagt
     7501 gaattataaa tcagtacacc gatttgaagc aaaacagcta aaaccatggc aatgagtggg
     7561 aaaatcgaag tcgtttgatt cgtcaatcct agtaattcca attttttcat cgctgaagca
     7621 tcaatttgat ttccttcgcg aacaatgatt tctccttgga agatcatcac tggttgtaca
     7681 gcttctcgtg cagattgttt taattcttct gtctttttct cattcaatac atcattgact
     7741 acaatgcctt gatccactaa gtagcggatc atttgttgct gctctgttgt gacattcaaa
     7801 tactgaatct gatcttctgc ttcttgttta aaagcttcca attcgctttc acgaacttgt
     7861 ttactcatct gttcgtcaat gagcttcaaa ctttcatccc taaccgtgtg aatttccgtt
     7921 tcagtcattt caaatatcgt cgaataaaaa ttgttcggca gtttttggta aaacgcaacg
     7981 ttttctgcat tcacgttttc aaaatttttc ttcagtgtag caatccgctc ttctgtagta
     8041 ggtttcggga tattttcatc ttccttcgcc ttatctacct tgtcattata agatttgttg
     8101 atggtatcat tcgtcttatc gatcaattcg aataattgct tgatccgatt gttctggtca
     8161 tctgccagat ctttttgata agtatattct ggagtcactg cttctgctgc tagttttctt
     8221 ctttgttcgg tttcttccgt gttctcgatc gttttattcg cacggatact ctcttctgct
     8281 acttggcctt ctctaaaatc attggccttt tgtttgacac tactaaatgt aacgccgcaa
     8341 acaatgataa aaaacaagaa cagaaggaaa ggcaatgctg cctttccgag tttttcttgt
     8401 agtttctgca gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat
     8461 gaagatccgc cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt
     8521 cacttgcttc aaaattaaca aaatcgattt ttttgatttg ctttagtgtt cgttctgcat
     8581 gaacaagacc acttgtcgtc cctcgaggca agtctatctg acttgtatcc ccattgacaa
     8641 tcatttttga ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt
     8701 tttgtgcttc gtccaagata acgaatgcat catccaatgt ccgcccccgc atgtaagcta
     8761 atggtgcgat ttcgattacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa
     8821 tttggtataa agcatcataa acaggccgca gataaggatc cactttttct ttcagatccc
     8881 ctggtagaaa gcctaggttt tcccctgcct ctacagcggg ccgcgtcaaa atgatttttt
     8941 gcacttcgcc tttttttaag gcagcaattg ctaatactac ggcaagaaac gttttccctg
     9001 ttccggctgg ccctacacca aatacgatgt catgtttggc taccgattca acatatttct
     9061 tttgaccgct gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct
     9121 catacatctc aacaaaataa tctaatgtac ctttttgccc cattttcaat gccgtcacaa
     9181 catcgggcgt acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg
     9241 aggcagccaa ggaaatctgg gattgttcac caatgatctg aatgatttct ccacgtgtat
     9301 ggatggttgt ttccgttgtt tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa
     9361 gcatgctgac atcatcagca gctgtcaatc taatctctaa agaactagac tcttccgtca
     9421 aaaatagaca tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa
     9481 ctatatagca aataagaaaa ggttgtaaaa gagtcgttta accatacgta ccaagaaacg
     9541 ttaaaaaata gccgtttcca ttcgctaaag gtctcgtttt gataaaaaga atctgagaat
     9601 gtaagttata tgaaagtgga gaacaaaata ttccgtttca ttcagcaggt attctctagg
     9661 tatttttctt gtttcaaaaa aagaaaacaa aatagttaaa atattcttgc atttcaaaaa
     9721 aaacaggtgt attattacgt caacaaataa acatacagca aagcgtcgag gaaaacagta
     9781 ggctattcaa cgatttattc ccagtgagtc atgtaagtga gaaatgacaa taaatcgaat
     9841 agacgaacat catttccgag tcaatgtcga aaacgtagat cattgtggct gcctccatta
     9901 tcaggctagc ggataacttt atcaaaaagc gtccgtgaat gaagaagctt ttttaagcga
     9961 aaaaaggtgg taccgttcta gaatctagag cccttttccc aagtggaaaa gggctttttt
    10021 tatattttaa ggtggtggtt ttcatggaat ctgatattaa tggcattact aattgctgca
    10081 acgtttatat cgtcaatcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa
    10141 aacttattca tggaaacaac tattgatggt cagttcactt atcttcggga tgttcttcgg
    10201 gtcaggtaat ctaatctttc ctgtccactt aggtcaaatg gctggcacaa actgggtcag
    10261 tgctggcgta ggatttgcca tctcaggcgc tcttttccca ttactagcta tcttagctgt
    10321 agtagtgaca aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc
    10381 tgccttgttc ttaatccttg ttcatttgac gatcggacca tttttcggta caccacggac
    10441 agctgctact gcttttgaaa tggctgctaa accatttttg cctgaaaaat ataattcatt
    10501 aggtatgttt ttattctctg ctcttttctt tttacttgct tatttcctta ctgtccatcc
    10561 cacacggttg gtcaaatacg tcggaaagta tctaaactcc ttgtttcttg cattattagc
    10621 aatcattttt gtcgtttctt tattccaccc aatgggaaat ctaaatcaat ctgctgcaga
    10681 tacttaccaa acaaacgctt taacaaatgg tgtcctagaa ggttataaca cggttgatgc
    10741 agtagctttg cttgccttaa gtgtcacttt tgtccatgca gtccgtgacc ttggtttcaa
    10801 agataaaaaa gtcacagaat taacagcaaa agctggtagc ctgagcgttg cgctagaagt
    10861 catgatctat tttggacttg ttcttctcgg tgcgatgagt ttgaataaga tgtctttatc
    10921 agaaaatggc ggaactgcac tttctcagat tactaatttt tatttaggta acttcggcgc
    10981 tagcttctta ggagtgatgg tcacattagg tgtcttcact accgctatgg gactcgttgt
    11041 ttcttttgca caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg
    11101 gttaacgact ttcgtctctt ttgtcgttgc aaatgctggt ttggacaata tcatccaatg
    11161 gtctttacca gtattgatgc ttttgtaccc attgtcgctt gcgttgatct tgctttcctt
    11221 aacagcaaaa tttttccaaa agacaccatt tgtctatcaa gtaacgatgt tgtttgctgc
    11281 tgttcccgcc gtattggata tgctggcaaa ttcgcctgct ttagtgagtc aacaaagagt
    11341 tgtagcaagc atgctcgaat tttatcatca tcacgttcct tttgctgcat taggacttgg
    11401 ctggatggtc ccaacgttac ttggttacgc tggcagctta ctcttctatt acgcctatcg
    11461 cctatcaggg tacaaacaag aagcaaatga actaccagaa gaataaccag atcaaacaca
    11521 atttaacata agaaatatta aacaggaaaa aaccagctag atgaaagaat cctattcttt
    11581 cgtctagcta ttttttagat ttctttttta cgagcacaaa aaaactgcgg acaatgattg
    11641 tccgcagttt aaatgataaa attaagattg taatagttct ttcacaatgg catttacttg
    11701 gtttccatct gcttttcctt tgactttagg catgatcgca cccatgactt tgccaaattc
    11761 tttcattgat gaagcaccag tttgtgtgat tgcttcttga acaagttgtc gaatttcttc
    11821 gtcggaaagt tgttccggca tgtatttttc gactatcaca atttcacttt ttactttttc
    11881 agctaaatcg tcacgtcctg cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat
    11941 ttcgcgagac aacaccgtta attcttcttc ttctgtcaag tcgtgacctt ctttaatctg
    12001 ctcgttttgt atagatgact tgatcattcg gatgacttgt aaagattcct tatctttcgc
    12061 tttcatcgct gttttcatgt catcgtttaa tctacttagt agtgacacac taccaactcc
    12121 aattcatcac agaactagaa ttttttgcgt tttctagctg cttcagattt tttcttgcgt
    12181 cttacacttg gtttttcgta gaattcacgt ttacgagatt cttgtaaagt acccgctttt
    12241 gacacggaac gtttgaagcg acgaagagca tcgtcaagtg attcgttttt gcgaacgact
    12301 gtttttgaca tgttaattcc ctccctccga gcttaaaaag taaccgtaat tgttattgaa
    12361 atatatctca aaaaacaaga ctgtcctgta aacattatat ctaatgctgt tttgaccgtc
    12421 aaggaaatct tgcttttttt taaaattatc aaatgttatt ttatttaaat ccttattgac
    12481 aattttcaca tcttccgaaa atctcgaatc tatgcccttc gatcgtacat ccaccgagct
    12541 gttcttcgaa ataattcatt ggacacatat ggatttcttt cgttgttccg cacaccgtac
    12601 agataaagtg atggtgatgg ccgatttccc cttgacagca atgaaaacga aacttcatct
    12661 ctccatttaa atcggtttct tccagtaaat cgatttctgc aaaatcatgg agatttcgat
    12721 aaatcgtatc gtaacttact cctttgaatt cctgattcat atagtcaaaa acttctttag
    12781 cggatacgta gcgattgcgc ttcactaaat aagttaacaa tgcttctctt tttttagtat
    12841 atttaaaacc gttatttttc aatgtttcta gtgattgttc gatcaatgtc tgttttttca
    12901 tgacttattc ctccaaatca aaatgattac gaagtacaca ttctagagta tggtataata
    12961 aacataaaaa aaatgcaagt gaggttttat ccatgtcttc cgattctaca gaaatcgtta
    13021 ctttttttgt catctccgac tctgctggag aaactgcgac aaaattagcg caagccacta
    13081 tggcccaata tcctagcgtt gagttcaatt tattccgcag aacatttgtg acagatgaga
    13141 aaacattgat gcaggcactg caagatgcac tagaagaaaa agctcttgtc ttacacacat
    13201 tgatcaatca agaattgatc gagatgacgc gttctttttg tgaagaacat caattattta
    13261 gttttgatgt gatgactccg ccgatatcag aaatcgagcg cttaactggt atcaagccca
    13321 ttcggcaacc tggcgccctt catttattga atgagaacta tttcaaacgg attaaagcaa
    13381 tggaatttgc tgtaaaatac gatgacggga aagatcctcg tgggtttcta gaagcagatg
    13441 tcgttttgct aggcgtatcc cgaacttcca aaacgccttt gagcttgttt ttagctaaca
    13501 aaaatttaaa agttgccaat cttcctttga tcccacaagc acatattccc aaacagcttt
    13561 gggagattga tcctaaaaaa atcgtgggat tgacgaataa cccagatatt ttaaacaata
    13621 tccgtaaaga acggatgcgc tcttacggat taaatcctga tactgcttat tctgatatcg
    13681 aaaaaatacg agctgaactc gattttgcaa atgatcttta tgaaaaactg ggctgcgtag
    13741 taatcaatgt cgcttcttta tctattgaag aaacagcttc gcttattttg aatgctttag
    13801 acctagaaga tcatagttat tatggaactg aaaccaccga agaaacagac taacagtttt
    13861 tcttgtcttt ttcttaaaaa aaactggact gtgtcagcga agaagattcg ttgacacagt
    13921 ccagtttgtc tctatttgca cctatcgtta ttgatgttcc acatgtttga gtgtctggtt
    13981 caaaatatcc agtacgtgag aatcatcaag actatacaaa atcgcttttc cctcacgatg
    14041 ggacttgacg acatggtttt ctcttaataa acgcagttga tgcgatacag cagattgttc
    14101 catttccact gcttgggtga ttgctgtcac attttgttcc ccgtctttca gatacagcag
    14161 aattttgaga cgagtggggt cactcaacat tttgaatagt tggctgactt tctctacttt
    14221 tgttttttgc gtgtctggca atccattcac ctccaacttg aaaaaagact gacttctaaa
    14281 gtttaccact ttttccatca gtctcaagtt tttttattct tctgtcatct tttggataat
    14341 cgcttcataa gcggctactt tttcagctgc gtcttctgaa gagtttcctt ttacaccgat
    14401 gtaaaatttg attttcggct cagttcctga aggacggata gcgatccagc cattttcttc
    14461 cagcgtatac ttcaatacat cagatggcgg tgtcgttagt ttttctacgt ttccttcacg
    14521 gtctgttctc gtcaactctt tgaagtcttc tgtttggatg acttttgctc cggcaaattc
    14581 tgttggtgct tcttcacgga atttttccat caaagcagag attttcgcag aaccttcttg
    14641 tccgctcatt gtcacagaaa tcgttttttc tgcgtagtag ccataggttt caaaaatcgt
    14701 ttgcaagccg tcatacaacg ttttgccttg ttttttatag taagctgcta cttctgctaa
    14761 taagagcaat gcttggatcg cgtctttgtc gcgaacgaaa ggtttaacta aataaccgta
    14821 actttcttcg aatccgaaca taaaagtatg ggaatgatct tcttcaaatt gttgaatttt
    14881 ttcagcaatg aatttgaatc ctgtcaatac attgaacatg gtagccccat agtgttccgc
    14941 aattgctgta ggtaattcgc tagaaacgat cgatttcaat accgctgcgt tttcaggtaa
    15001 agtgcctcgc gattggtgtg cttccaaaat atattgagca aacagtgctc cgatttggtt
    15061 tccggtcaat acttcatagg agccatttgg caaacgaacg gctgcaccta aacggtctgc
    15121 atcaggatct gttgcaatca gcaaatcagc gccttctttc tcgccaagtc gtatcgcata
    15181 ctcaaaagca gagtgttctt ctggatttgg tgatttgact gtagtgaaat ctgggtctgc
    15241 gacagcttgt tctggaacta gtacgaattg ttcgaatcca gcttgtttca acgcacgttc
    15301 gcccaacatt tttcctgttc cgtgaagtgg tgtgtacaca agtttcaaat ctttccccat
    15361 tgtttccacc aattcatgat caatcgttac tgctttgatt tcttctagat aagcggcatc
    15421 cacttcttct ccgatgatcg tgatcaatcc gctatgttca gcttcttcgt cagataatac
    15481 ttcaactttt aacggattgc tgacttcacg gacatatttc gtcaatgcat ctgcgtctgc
    15541 aggaggcatt tgtcccccat cttcgccgta gactttatat ccgttgtaag ctgctggatt
    15601 gtgggatgca gttaccatga ttcccgcaaa cgtcttcaaa tgacggactg caaaagaaag
    15661 ctctggtgtt ggacgtagac tttcgaatac ataagatggg atattgtgat gtgctaatgt
    15721 tttggctgct tccatggcaa attctggtga ttgatggcgt gagtcataag cgatagcgac
    15781 acctcggcgt tttgtttctt cgccttgtgt atccatgaaa cgtgcaagtc cttctgttgc
    15841 ttgtcttacc gtatagatgt tcattcgatt gatcccaggc cctaagattc cccgcatgcc
    15901 tgcagtaccg aattccaacg gtgcataaaa agcatcttca agtttttcag gatcttcacc
    15961 cagatcattt aattgttttt ttaagttttc ttctaaattt tcttcatgta cccattgttc
    16021 atacacttgt tcccaagaca tgaaaataac acctctttct gaaatatggt tcttaataag
    16081 tatagcattc acttattaga tataaaagta tgaggaccaa aaaacgctta aatcaagaaa
    16141 ggattctttt atcaagcacg gttgcaagta ttcgcataaa tcggattcag tagatttcac
    16201 gcgaaaaaaa agagtaaaag aacaattgaa accgttcttc cactcttttt aaacttatgc
    16261 agttgtttct tctaaagatt ccaagtattt atacacttgt tgtccagcga tcccaccatc
    16321 accaacagca gtagtgattt gtcgcaaatc tttttcacga acatctccga tggcaaaaac
    16381 gcctggtata ttcgtcttca tttcgttgtc tgttggaatc cagccagctt cgttcgtcaa
    16441 ccctgcttct ctaaatggtg cagttaatgg atccagtcca acatagatga atactccgtc
    16501 tgctgccaaa tcaaactcat tgccggtttt gacattacgt gctttcacac ctgtcactac
    16561 catttcattc ccaacgattt cgtcaacgac tgtatcccac ataaaggaaa ttttttcatt
    16621 cgcaaatgca cggtcttgga tgatcttttg tgcacgtaat tcatctctac ggtggacgat
    16681 gactacttca gaagcaaatt gtgtcaaata gatggcttct tctactgctg agtctcctcc
    16741 tccgatcact actagacgtt tgttacggaa aaacgcacca tcacaaactg cacagtaaga
    16801 aacgccacgt ccagcaaatt cttcctcgcc aggtacgcct aatttacggt ggatacagcc
    16861 tgttgcgata atgactgttt ttccttcgta agttttatct tcacaaataa cttctttgtg
    16921 gctgccgtga tccttgatat cttgcacgat tccataagca ttttctgtcc caaatcttga
    16981 aacaccttca tacatcttat aagctagatc aggacccatg attgaatcga aaccaggata
    17041 gttttcgatt tctgccgtat tgttcatttg gccgccaggg gcgccttgtt cgatcatcaa
    17101 tacggataag tttgaacgtg aagcatataa agcagcagtc atcccagcag gacctgcgcc
    17161 aataacgatt acatcataca tatgtagatt ctcctttact taatcaagta agcatacttt
    17221 acagccaatg cgaaaaaaag tctattgttc tgcttgcgtt tctcgaatag ctttattata
    17281 ctcgaaaaat ccgctgaaaa acaatagact tttataaatc attgatcaat cattcgaaag
    17341 attttctcca tttgaatcaa ttactttttt ataccagttg aatgatttct tcttcgaacg
    17401 ggctaatgtg ccatttccct cgttgtcacg atcgacataa atgaatccgt accttttctt
    17461 catctcacct gtaccagctg atactaaatc aatacatccc caagtcgtat agcctaatag
    17521 atcgactcca tctaattcta ctgcatcttt cattgcttgg atatgtgctg ccagatagtc
    17581 gattcgataa tcatcttcca cataaccatt ctcatcgggc gtatcgactg cacctaagcc
    17641 gttttctaca ataaatagtg gtttttgata acgatcataa atatcattca tcgttattct
    17701 caagcctaat ggatcaattt gccaacccca ttcacttgct tccagatatg gatttttgac
    17761 agaagcaaag atatttcctg cagtctgttc caagagttcg ttgtcagtag tcgaaactcg
    17821 agaagaataa taagaaaagg agataaaatc aacagtatgt tctttcagca atgcggcatc
    17881 cccaggttcc atttcgatct gaatgccatt tcgttccatt tctttcaatg cataagcggg
    17941 atattctcct cgtgactgga catcaatgaa gaaataattt tctcgatctg attttctaga
    18001 agcccaaaca tcttcaggat tgcaagtgta tggataatat gctcctgctg ccagcataca
    18061 gcctactttg ttctccggat ctacttcatg agcaatcttc gttgcgatgg cacttgcaac
    18121 caactcatga tgtgccgctt gatatttgat ttgttcttca ttttctcctt cttcaaaata
    18181 cagccctgcg cccatgaatg gtgcatgaag gatcatattg atctcattga atgtcaacca
    18241 atatttgaca agtcctttat agcgattgaa gatgactcgg cacagatttt cataaaaacc
    18301 aacgagttct ctggaacgcc acgcaccata ttctttgatc aaatgcatcg gacaatcaaa
    18361 atgtgtaatc gtcacaagcg gctcgatccc gtacttttga cattctttaa agacatcctc
    18421 ataaaatttc aacccagatt catttggctc tttttcatcg cccattggaa aaatacgact
    18481 ccaagcaatg gacatccgat aagtcttgaa ccccatttct gcaaataaag caatatcttc
    18541 cttataatga tgatacatat cgatcgcaat ttttgctgga taaaaatgat tttcatcaaa
    18601 ttcaaacatt ttcttttttc cagtaatgac aggaaaacgg tcttctccga ttggcacaac
    18661 gtctacgttc gctaacccac gtccgccttc atcataaccg ccctcgcatt gattggctgc
    18721 tgtagctccg ccccataaaa aatcttttct gaatcccatc atacttcctc cattacatgt
    18781 gtattgcttg ataattctct tgccagttca tcttggactt cgattccttc tgcttttaac
    18841 cggctgattg tttccttgaa ttgcggtaaa tactgctgat gtgcaataaa caactcatcc
    18901 attatccgtt ttgctgtctt tcctgatcgt ataagcggat tgatcgtaaa cgcttgcaaa
    18961 gctgttccat aatcaccagt cacggctgct tcgatcgtca gcaattccat attcttcatt
    19021 acttgcaacc agcctctttc agcagcagga agggaaccaa acgctacatt tcgtgcgcct
    19081 tgtgcgccaa cataggcagt gacttctact acacaatctg ccggcagatc aggaacagct
    19141 ccatcattct ttgtagatac cacgatctgt gtttctttat ttccatagat cgaagcaatc
    19201 gtttcacagg cagcatctga ataataagca ccacctcgtt tttgcaattg ttcaggttta
    19261 tgatccaatt ctggatcttt gtataattca aacagctctg cttctgtttc tttcacttgc
    19321 tgcgctcttg taccaatcgt ttgatattct tctaaagcat gttccagcat ttcttcttca
    19381 cggtagtaat agcggtggta gccacaagga atcatgttca tttgttttaa ttgttctttg
    19441 aaaaacggga tatcaaagat atttttcggc aatccattgt cttcatcgta taaacgatca
    19501 atcaattctg ccgtgcggtc atttccttta ttatctgcta ctttgtgcca atggaagtga
    19561 tttagaccgg caaacttgta gatcagctct tcttttttct tgccaagcat tgttggttct
    19621 gtcatcatcg ccattactgg cacattgcat aatccgatca ctttatccca tttgccatat
    19681 ctgataactg cttctgtcac catcccgctt gggttcgcga aattgatcag ccaagcattt
    19741 ggacataaac gcttcatgtc ttcaacgatt gccaaaacca ctggaacggt acgaaaagct
    19801 ttgaatatcc cgccagcacc atttgtttct tgcccaatca tcccataata actagggatc
    19861 cgctcatctt tgattcgtgc atctaattga ccgacacgaa actgagtcgt aacaaaatcc
    19921 gcatctttca atgcttcttc ccgatccaga gttgcataga ttttgacttc atacggagaa
    19981 gcatcccaca tacgctgaga catctgactg acgatctcaa gcttttcttt tccttcttct
    20041 acatctacga gccagatttc tcgtatcggt aattcctcat atcttttgat aaatccttcc
    20101 atcaactcgg gggtgtaact gctgcctcca ccaatcgtag cgattttgat tccttttgcc
    20161 atgttctcac tcctttactt tttacacttt tattatcttt tttgtaaaca aaacaaacaa
    20221 tagaaattcg tttctttcca gtaacattta caaaagttag tgttttcttc tggatttttg
    20281 taaatatctt gtcaaaaaat gtctttatta agctatataa cataaatccg tttacaaatc
    20341 attttcaaaa aagttataat ttacgtaaaa aaacaagaag aaggtatcta catgttatta
    20401 cgtgaaaaaa tcaaagaaat ctccttttct ccttcagaaa atgaagtgat tcagtttctc
    20461 ttaagcgatt ctccagcact tggcgaaatg accatccaag agattgctga gcgatgttat
    20521 gtccacccgt ccacactcat ccgtgtagca aaaaaaatgg gttttgatgg ctgggtcagt
    20581 tttcgtaatg cttttttagc tgaaacagcc tatcttgaag ggaattttca agacgtagat
    20641 gcgaatcttc ctttttccac cacagacggt attatgacga tcgcaaataa aatggcttct
    20701 ttagaacaga tgacgattca agatacactt tctctcattc atcatgacca actgcaaaaa
    20761 gccaaacaac taatgcttca agcaaaaaaa atcgtgattt tcgccagtaa ctcgaatact
    20821 ttgatttctc aagatttcat gctgaaaatg aagcgcatca aaatggatgt cacgattgtc
    20881 accacttttg gtgaatcgtt ctatgaagcg tacaattgca cgagtgatat gtgcgggatt
    20941 ctgatttctt atacgggtga aaacaatgtg atccttcgta ccgcagaaat attgaaacaa
    21001 aatcatgtgc caattttagc tttgaccagt atcggtgatt caacgctatc ttcaatgagt
    21061 acagccgctt tgcgacttac gacacgcgaa cgactgtatt cgaaaattgg taattttacg
    21121 atcaatacat cgatttgtta tcttctagac gtgttatata gttgtgtttt tgcagaaaat
    21181 tatcaacaga atttagtcca tctaatttct gtaggacaaa aatttgataa acgccctatc
    21241 agctcagcca tcatgaaaga gcctttcaat tttgaaaata aagaagtgga ctaaaaagaa
    21301 taattaagcc tagtccattc tttcacctaa ccgttcaggt cagtgcggtg ataagtgcat
    21361 cgtctggccc gaccgtctcc ccttgatttt ccaaaatatc caaataatcc gtgtaattgg
    21421 tgacgatcac aggtgtttct aaactgtatc cttcctcttc gattttcttc ttgtcgaagc
    21481 ggagcaacag atcgccttgt ttcactcttt ctccttgctg caccaagctt ttaaaatatt
    21541 ttccttctaa ctgaacagta tccaatccga catgaatcag caattccaga cctgcttcag
    21601 aaaccattcc gattgcatgt ttcgtcggga acagggtcat gactgttccg tcaaaagggg
    21661 cacgtacttc tcctttttca gggtagatca agattcctcg acctaatgtt ccttgtgcaa
    21721 aagctgcatc ctttgctgca ttcagtggag cgatttgtcc ttgtatcggt gtttttacaa
    21781 tttctttacc tgatgttttc tgtctatcat ctgtttcatc tgcttcctcc ttccaaaaga
    21841 aaaaagtcag cgcaaaggat accaccatcg caatggcgat cccaatcaat acattaatca
    21901 catctgttaa attaccatct ggtctaatat agttgggaat tgtaaagatg cctagtccac
    21961 cttgattcca agaagttaaa ttcattaacc cactatacgc gccccctaac gcaccaccga
    22021 cacaggtaaa aataaatatt tttttacgtg gcaatgtaaa cccataaata gctggctctg
    22081 taatccccac aataccagaa acaatggctg gtattgcaat tgctttgtct tttggatttc
    22141 taagcttaaa gtagagtgca gtaagtgctg cagtttgtcc aaaagacact actgcaacag
    22201 gtgtcatgat attttgccag ccattcacag aaaattgcat caatcccatt ggtacgattg
    22261 cccaatggag accaaacatc attagaattt gccagaagaa gcctacgatt gctccataaa
    22321 taactggcga aagtgctaaa agtgtttcaa atccagccat taaaatatca gttgcgaatg
    22381 tgaatgctgg tccaacgagt aaaaaaccta agggcattgt aataagaacg gtgaagaatg
    22441 gaacgaagaa attcgcgatc atctcaggta ctaacttttt aattggtttc tgaatttggc
    22501 tagctaacaa cacaataaag ataatgggaa tgacagaaga agcgtagtta cgcgcaatcc
    22561 agggaatgcc gaatactgtt tgataaatag gtgcttcaaa aatagtgcca gaaaatatgg
    22621 tcgtcagtgg tgcttctgct gcaccactca aagcttccag cgacaagcta ggattcatta
    22681 atgctgcccc aagcaacatc cctacgaatt catttacttt aaatttacgg gcagctgtta
    22741 aaccgataat aacaggcata aattggaata cagaatcacc gatattatca aacataacaa
    22801 atgtgccaga accgcggtca aatgcgccta agaagacaag taatgctgtc agtccacgaa
    22861 tcatcccacc agcagctaga atagcaagga atggctggaa acagcccgat attccatcaa
    22921 taagcatatc aaacggtctc attttttctg ttgaaacagc ctcatcaaaa gtaaggtttc
    22981 ctgctcttat aacctcttca aacacgactg gcacatgatt cccgatgaca acttggtact
    23041 gtcctcccgc tcgcattacc gtcacaacac cgtccatatt tttcaagatc tcatcatttg
    23101 ctttttcttc gttttttaat ttgaaacgaa gccttgtgat acaatgtgtc aaaccattga
    23161 tattttcctt cccgccgaca ttttctacaa ttttgctcgc taattcttga tattttccca
    23221 tttctattcc ctccaattta ttagtggaag gtttagccaa ctgaatgtca ctacccttat
    23281 ttcatgcgtt cttgttaatt tatcctcctt ttttatttat ttacaacgat tctcatgcac
    23341 taaacgcgca gtatggatcg ttaaatacat tttttcgtcc gtggacatct ggtagttgaa
    23401 tatctcggct aaaaagtctg ctattttctc aacgcaacca aacgtatcct tatatttttt
    23461 ttgaataact tggtacaatt cctcagaaac ttctcccact tctgattttt ctccatctag
    23521 gacgcgtgtc agaaaaaaac gtaggtgtgt gacgaatcga aaaaaatgga ctgaatcttc
    23581 atcaaatgta atcttatagt aatagcggac gatatttaga atatcctgta ttgttgtagt
    23641 aaacaggtag gtattgtctt gttggctttg aatgccttgt gcattgacta aatgcaatgc
    23701 gataaatccg gcttcatctt ctgataactt gatgtggtat ttatccgaga ttttcttcac
    23761 tgttttttcc ccgatggcat actctttagg aaatagttgt ttgatatcat acagcaatac
    23821 atttggtaca aatattccca ttttgtgacg ttcgatagcc gtatacaaat gatccgtcaa
    23881 tgcaatatac actgaatcat tcaactggac ttgtagagcc tcttttgtat attcaatgat
    23941 ttcacaagaa atttctaagt actcaagagg aatcgcttgt gccaattctt ggaactgttc
    24001 tgaaagtccg tcatttgtca actgaaattt tttgtcgatg catgagtcaa aaatcgggtc
    24061 acccactttc tttttgaatg ccaaaccttt tcccatcacg atcatttcct gatttctttc
    24121 atctctggtt attacgacgt tgttattcag tactttctca atcagcatgt tttactcctt
    24181 tccatgatat atagggctgt atccaaaaca aaaagaaaaa cccaaaaagc cgtccatgga
    24241 accatgggag actcatcagg tttagcttgc atcaggcaat cactatccaa gttgtatcgt
    24301 tttctttata ttatcgaaag caagcgcttt tgtcaacgct ttcttagtgg ctattaggca
    24361 cttctgacta gctgtcgaat cttttttaaa tctggtgact acgacactac cgctcacaga
    24421 tgaagaatct gggaagctct gttttatttt cactctagcg cttaggtcaa aatgctacta
    24481 tgctactgtc ccaacttcta atatacggtg ttccagtatc agcttatcgg tgataagcca
    24541 aatttatgca aaaatctgga aagccttttt tacaattttt ttcttatcac ctaaagctga
    24601 acggagccct gtcacaacca ctttttacac accactgctt tgcagtttgt acatatctgc
    24661 atacaaacca ttttgcgcta gaagtgtttc atgattcccg cgttctacta tatgaccttt
    24721 gtctaaaact aaaatcaatt ctgcatcccg aatcgtggac agccgatggg caatcgcaat
    24781 cgttgtccgt ccttgtcgca tttttgtcaa tccttcttgg atcaatcctt ctgtttcagt
    24841 atcgatattt gctgtcgctt catccaatac tagtatttta ggatctgtca cgattgttcg
    24901 cgcaaaagtg atcagctgtc gttgtccgct agaatagctg gcaccgtgtt cgatcacttt
    24961 tgagtgatat ccttttggta attcatcgat gaacttgttt gcttgaacaa actcagctgc
    25021 atggcgaatc tgttcgtctg tgatagttgg attcatcatc cggatatttc ctgcgatatc
    25081 tccataaaac aagaaggcat cttgcaagac caatcccatt ttcttacgta actcttccat
    25141 tggatattct cgaatgtcat gatcatcaat caagatctgg ccttcataaa attcgtaaaa
    25201 ccgcatcaat acgttgatga tcgagctttt tccgcttccg gtgtgcccca ctaatgcaac
    25261 ggtctctccc ggattagcga caaaagagat attgttcaat acattgtgct ctccatcata
    25321 agaaaagctt acattgcgaa actcgatctt acctgaagta atcgtttgat ctgctccagg
    25381 attttgttgc ggtgcatatt cttctgtatc catgattttc aaaatacgac taccggcaac
    25441 cattccatct gtaaaggtgc tcaaaaagtc catcatttga gacattggat taaaaaatcc
    25501 ctggacatag gtcgtaaatg catagatcag tccaacttct acgggagaat gcagcgcatc
    25561 gatcccaaac aaagtcaacg ccaacgcaat cgccaatgca tatagaaaat tgatgatcgg
    25621 acttaacaga atcgaattcg ttttgatcat cgcatagcgc gtgtctaaat actcgttgtt
    25681 cacttgttca aattctttag ctaaacgagc ttcttggcga aactgctgga taatacgcat
    25741 gccggaaatc gattcattca atttcgtgtt cagctgactt aatttttccc gcatattccg
    25801 gtaaatacga gaactgaatt tctggtaata ccagatgacg attcctaaaa tcggcaagaa
    25861 caccaaagtc cacaaggcaa tttctccatt gatttgaaac atggcaatga aggaagaaac
    25921 gactgcaaag atcccagtca ataccatcaa aaagacatac caaaattcaa atagcgtttc
    25981 tgtgtcattt gtcactcgag aaacggtcga tcctgccggt gtctgatcaa aataacgcat
    26041 acctagtgta tgcagctttt caaataatct tacccgaaca tactgatagg tcttcaatga
    26101 agccatggaa tacaaatacc attggaaaaa ccaaatgata cttttgacta tgactccaaa
    26161 caagtacata ccagcaaaaa acaaaataac ttgtgtcgta gccgactgtt tagccagatg
    26221 atcatccata aaagtttgga tgatccttgg caataggaca ttgatcacag acagagcaaa
    26281 cgcaaataag atagcaacga tgaacgttct tctaaaaggt ttggcgaact tcatcagtcg
    26341 tttgatgatc ttcttctgtt ctttaagtgg aatcgatttt gtccattccg attgataatt
    26401 ttcatccatc aggcctcgcc tccttctatc ttcgcttcta attgctgttt ctcccacatt
    26461 cgttcatacc agccgcctaa tgcaagcagc tcatgatggg ttcctcgttc aatgattttc
    26521 ccttgatcga caacaatgat ttcttttgca tgcatcacgc tgcttaaacg gtgagctgca
    26581 atgatggttg ttttctcttt tcttatttct tttaaattat gcaagatagc ttcttctgtt
    26641 ttggcatcta ctgcagaaag tgcatcatct aagatcagca attcaggatt tttgatcaat
    26701 gctcgagcaa tcgaaatcct ttgtttctgt ccgccagata aagagactcc acgttctcca
    26761 actaaggtat cataaccttt tggcaattcc ttgatttcat tgttgataaa tgctaaagaa
    26821 gccgcatgct ctacttcttc ttctgaaagt tttggattag aaaaacggat attatctcga
    26881 atggtcatcg aaaacaagaa gtgatcttga ggcacatagc cgattgatcc taataaagca
    26941 ttcaatgaat attctttgat cgaacgtccg ccaaattgga tttttccttg ataatcatcg
    27001 tattcacgca tcagtaattt gatgatcgtc gtttttcccg caccagtttt cccaacaata
    27061 cctaatgttt ctccttcttg gatcacaaat ttgatttgtt ctaatgctgg tgattcttct
    27121 ttcggatagt taaaagagga aatagctaca ttcagctcgc cttttgccgg ggtctgcact
    27181 gcacccagtt gttccacgat atgcgtcttt tcatgcaaaa gttcattgac gcgatcataa
    27241 gaagcatttc ctctttccaa tacgttgaac aatcgaccaa tcgcaaacat cggccaaaca
    27301 agcataccga tatagctaat aaatgagacc atctgcccga tcgagatcgt gccttccaag
    27361 acatactttc cacctaaaat aattgttgca acataagaca gaccgataat caaggtaatg
    27421 aatggatcaa ataaggagtc taaaaaattg actcgtttgt ttttaataat tgcatcttca
    27481 atcttttctt caaaatccgc gagatcttct ttttcttgtc caaatgtttt aatgactttg
    27541 attccagtaa tactttcttg tgtcttatca ttgatactag aaaaagcttc ttgcgaatca
    27601 cgaaaagcat cgtggagttt tgaccccagc actcgtgaag taacggctag taaaggcaga
    27661 ggaatcagcg caatcagtgt taagcgccaa tcaataaaca aaatcatcgc aatgatcgtt
    27721 gttccgcccg tgatcactga atctgcaaag gtcaagatac cagctcctgc tacattttgg
    27781 attgcattca aatcattcgt tgcatgagcc atcaaatctc cagtccgata tttttggtaa
    27841 aagagattgt ccatttctgt aaaatgtttg aatagctgct gccgaagttc tttctccaac
    27901 cgagctgcac ttccccaaat attcgtccgc cagatataac gaaaaagata ttgtaagaga
    27961 gctgcaagga tcaataataa gatccaccag gaaatgatct ttattgataa atttttatcg
    28021 gcaatctcat caatgatgat cccaatcacc tttggaggaa ctaactgtac caaggccact
    28081 gcgaataggg agaaaatccc aattaaataa tgtttctttt cctgtcgaaa aaaccaacct
    28141 aattttctaa aaatcgacat tttactcctc ctttgtcttt atcaagagca taggattgtc
    28201 tgtgcttgtc ttttttgctg aatagtctga atagtctaac aaattacgaa ataaaaaacg
    28261 gactgaaaaa atcagcccgc aaaaagtctt tgggtcagaa cctatttttt cccttggttc
    28321 ttcatttgct gcatcatctg gcgaattttc ttctcagatg gtttttgccc cattgacatc
    28381 atcattgatc gcaacatgtc ctcattaacg ggaggatttt tttctaaata gtctttcata
    28441 tatttacgcg ctaggaaaaa gccacctact gcgccgatta gcaaagcaat aatagcaata
    28501 agtacgacaa tacctgttga taccatataa atctctcctt tctatttccg aattgctctc
    28561 aatgatttat tttactaaag tttattgaaa aaagaaaggc gtattcttta gtttcatgca
    28621 aaaaatagtg gtttttttag agaagaacaa aatttctttc attaaccagc aagacacgcc
    28681 acgaaaaaaa attaggcata ccgaatattt ttctttaatt ttttctttct gaggtgtaga
    28741 atgtaatcgt agataaatag gaaaggtggt tttttcttga ataatcaatc caacaaacaa
    28801 tttcataagc atcgacttat tgaacataca aatggtttat ctttagctga agtaaatgga
    28861 acagtggatg tacccaaggg aaaaagtttt tttaaaacac ttcttgctta ctctggacca
    28921 ggtgcattag tagcagtggg ttatatggat ccaggtaact ggtccacttc gatcactggt
    28981 ggacaaaatt tccaatattt attgatgtca gttattttga tgtctagtct gattgctatg
    29041 cttttacaat atatggcagc aaaattagga atcgtgaccc aaatggattt ggcccaagca
    29101 atccgagcac ggacaagtaa gacattgggt gtcattttat ggatcttgac tgaattagcc
    29161 attatggcaa cagacatcgc agaagtcatt ggtgcagcaa tcgccctcta cctgcttttt
    29221 gatattccat tagtgattgc cgtatttatc actgtgttag atgttttcgt cctcttattg
    29281 ctgacaaaag tcgggtttcg taaaatcgaa gctttggttg tctgcttgat tttcgtcatt
    29341 ttattcgttt tcgtctatca agttggcttg tctaaccctg attggcacag tttgtttgcc
    29401 ggctttatcc ctaacagtca tacattctca gactcaccgg tcatcggcgg tgaaactccg
    29461 ctgactggag ctttagggat tattggtgca acagtcatgc cgcataactt atatcttcat
    29521 tcagcagttt ctcaaactcg gaagatcgat caccaagatg aagaagatgt agcacaagcg
    29581 gtacgctttt ctacttggga ttctaatatc cagttgtcaa tggcttttct agtcaatgca
    29641 ctgctattga tcatgggtgt cgctgtattc aaaaccggtt ctgtccaaga tccttccttt
    29701 tttggtctgt atgaagcttt atctgatcca tcagctatga gcaatggtat cttgattgct
    29761 gtcgcaaaat ctggtatctt gtccacttta tttgctgtag cacttcttgc ttctggccag
    29821 aactctacta ttactggtac gttgaccggt caagtcatca tggaaggctt catccatatg
    29881 cgtatcccga tttggctaag aagactagtc acacgattgc tttctgtgat tcctgtattg
    29941 atttgtgttt tgtttacaag tacaaaagga acgatcgaag aacataccgc tttgaataac
    30001 ttgatgaatg aatcccaagt ttttctggct tttgcattgc cattctcgat gattccttta
    30061 ctgatgatga ccaatagcca agcggagatg gggagccgat tcaaaaattc atttgttgta
    30121 aaactacttg gctggttctc tgtgattagt ctgacttact tgaacttacg aggattacca
    30181 ggtcaaatcg aaagtttctt cggcgagcaa gccacctcag ctcaactagc attggctgat
    30241 caaatcgctt atgtaatcat tgttcttgtc cttctcctgt tggcatggac gattggtgat
    30301 ttatataaag gaaacaaaaa atatgaacag cgtttgcaag aaacgataga agaaatcgaa
    30361 tagaaactga ttcttttaca caaaggaggt tgtga
//
LOCUS       DACEMO010000013        29475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEMO010000013 DACEMO010000000
VERSION     DACEMO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.013"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..736)
                     /locus_tag="IW728_000431"
     CDS             complement(17..736)
                     /locus_tag="IW728_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1023813.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            1073..1405
                     /locus_tag="IW728_000432"
     CDS             1073..1405
                     /locus_tag="IW728_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023814.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            1392..3395
                     /locus_tag="IW728_000433"
     CDS             1392..3395
                     /locus_tag="IW728_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1023815.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFYKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            3414..3884
                     /locus_tag="IW728_000434"
     CDS             3414..3884
                     /locus_tag="IW728_000434"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1023816.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            3905..4489
                     /locus_tag="IW728_000435"
     CDS             3905..4489
                     /locus_tag="IW728_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1023817.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            4504..5490
                     /locus_tag="IW728_000436"
     CDS             4504..5490
                     /locus_tag="IW728_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1023818.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            5480..5791
                     /locus_tag="IW728_000437"
     CDS             5480..5791
                     /locus_tag="IW728_000437"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1023819.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            5813..7594
                     /locus_tag="IW728_000438"
     CDS             5813..7594
                     /locus_tag="IW728_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1023820.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            7587..8963
                     /locus_tag="IW728_000439"
     CDS             7587..8963
                     /locus_tag="IW728_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1023821.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            8970..9605
                     /locus_tag="IW728_000440"
     CDS             8970..9605
                     /locus_tag="IW728_000440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1023822.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            9635..10990
                     /locus_tag="IW728_000441"
     CDS             9635..10990
                     /locus_tag="IW728_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1023823.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(11182..12576)
                     /locus_tag="IW728_000442"
     CDS             complement(11182..12576)
                     /locus_tag="IW728_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1023824.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            12668..13639
                     /locus_tag="IW728_000443"
     CDS             12668..13639
                     /locus_tag="IW728_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1023825.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            13636..14418
                     /locus_tag="IW728_000444"
     CDS             13636..14418
                     /locus_tag="IW728_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ1023826.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(14587..14958)
                     /locus_tag="IW728_000445"
     CDS             complement(14587..14958)
                     /locus_tag="IW728_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1023827.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            15092..15535
                     /locus_tag="IW728_000446"
     CDS             15092..15535
                     /locus_tag="IW728_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1023828.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(15578..16387)
                     /gene="yidA"
                     /locus_tag="IW728_000447"
     CDS             complement(15578..16387)
                     /gene="yidA"
                     /locus_tag="IW728_000447"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1023829.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16406..17773)
                     /locus_tag="IW728_000448"
     CDS             complement(16406..17773)
                     /locus_tag="IW728_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1023830.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(17757..18587)
                     /locus_tag="IW728_000449"
     CDS             complement(17757..18587)
                     /locus_tag="IW728_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1023831.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            18750..19187
                     /locus_tag="IW728_000450"
     CDS             18750..19187
                     /locus_tag="IW728_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1023832.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            19263..19826
                     /gene="rpoE"
                     /locus_tag="IW728_000451"
     CDS             19263..19826
                     /gene="rpoE"
                     /locus_tag="IW728_000451"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1023833.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDDEDEEIAAYNSDLQEIGADDPTDDEEDLPGNI
                     EEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            20015..20521
                     /locus_tag="IW728_000452"
     CDS             20015..20521
                     /locus_tag="IW728_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1023834.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            20538..21437
                     /locus_tag="IW728_000453"
     CDS             20538..21437
                     /locus_tag="IW728_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1023835.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            21524..22408
                     /locus_tag="IW728_000454"
     CDS             21524..22408
                     /locus_tag="IW728_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1023836.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(22600..22671)
                     /locus_tag="IW728_000455"
     tRNA            complement(22600..22671)
                     /locus_tag="IW728_000455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22636..22638),aa:Arg,seq:ccg)
     gene            22731..24065
                     /gene="rpoN"
                     /locus_tag="IW728_000456"
     CDS             22731..24065
                     /gene="rpoN"
                     /locus_tag="IW728_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1023837.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            24268..25305
                     /locus_tag="IW728_000457"
     CDS             24268..25305
                     /locus_tag="IW728_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1023838.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            25346..26347
                     /gene="gap"
                     /locus_tag="IW728_000458"
     CDS             25346..26347
                     /gene="gap"
                     /locus_tag="IW728_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1023839.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            26457..27647
                     /locus_tag="IW728_000459"
     CDS             26457..27647
                     /locus_tag="IW728_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1023840.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            27751..28506
                     /locus_tag="IW728_000460"
     CDS             27751..28506
                     /locus_tag="IW728_000460"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1023841.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            28622..>29475
                     /gene="eno"
                     /locus_tag="IW728_000461"
     CDS             28622..>29475
                     /gene="eno"
                     /locus_tag="IW728_000461"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1023842.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPI"
BASE COUNT         9669 a         5193 c         6329 g         8284 t
ORIGIN      
        1 cccctcagaa agtctattaa aattcattca caaagaaatc gaataagcgc tgttcagatg
       61 gatgtacacg gtgggtaagt tccggatgcc attgaacacc gaggatttta tgcatatcat
      121 ctttgctttg aaccgcttca acgagtccat cgttagaaaa agcgatggct tttaataaag
      181 gtgaaagatc tttgactgcc tgatgatggt aagaattcac aaaatatgga tcggggagaa
      241 ggttgcttaa caaactgtct gaaacaatct tgacttcgtg agtcgtaaat tgcggggcag
      301 tgggctgttg ttcatgtttt actgaccact ttggatataa tgaaagatct tgataaagtg
      361 taccttccaa aacgacattc aatagttgga gtccccggca gactgcaaaa attggttttc
      421 tttgtttcaa tgcttccaag atcagtgcct gttcaaattg gtctcgttga atatttgttt
      481 ccgttagctt tggatgagga tcttccatgt aaagttgtgg actgacatct tgaccacctg
      541 caagaataag tttgtcgatt tgtccaatca gttgaggagc tgaatctgga gacatgactg
      601 gcagaataag aggcaatcct tgagcatcta aaactgcatc cacgaatcct tgcggtgtat
      661 agctcacttg atttccttgg aaagtatctg ttgctcgaat cagttgattt ccggcaatgc
      721 cgatgattgg tttcatttgt attcccccta aagtagactt attgtaacaa ggatttaaaa
      781 aaaattaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg
      841 taaagagaaa tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat
      901 aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa
      961 ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac
     1021 atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca
     1081 agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaaa agcgtgctca
     1141 aacactgcgg cagcagctga agatctatgc aacagaaaaa gagcaggaat tggaaacgat
     1201 ccaaacaaac tatcgacaac aactaaaaca gttggtggaa gaaaaagaac aatttgaaat
     1261 acaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac
     1321 tttgaaagat caatatcaaa agcatcagag tcaagcgatc ggtgcgatca ttgaaagggt
     1381 gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa
     1441 atcaggagag gattttacaa gcagtacaag gaatccaaaa cgttgaaatc cgggatctgt
     1501 atcaaaatag aacaaacaac cagtgggtgg agaagtattt tccagatacc acgattattg
     1561 ataaagaagc aaaattaagc gctttaggta atcggttgaa agacatccaa gaagctatcc
     1621 aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac
     1681 tttccttaca agatttggaa gccaattatt ccgaagaaaa tttcataaat aaattgaaag
     1741 aagtccttga tttaaaagaa aagtgggaac aactaactga agaacgtgat cgtttagaag
     1801 aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa
     1861 gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg
     1921 aagaactgag taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag
     1981 tttatctttc gtatgttgct ttaaaaaaat cagttgctgt tatagaagaa attgctggga
     2041 aatacgggtt ctataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaactgcaag
     2101 agaccaagca gcaattatcc attgttcacg agcagcaaaa gaagataacg acagcgttag
     2161 gtcagtgcag tggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg
     2221 cagaacgaga aaaaataaaa gaacggttta tccatgcgac ttatctgatc gttttgcaag
     2281 gttgggtaga tgcagaagaa aaacagaatt tgctgcatgt gttaaacgaa accgtatcag
     2341 aagaagatat atacgtcagt ttcgaaacac caaactcaga agagattcag caggaagtcc
     2401 ctactaaatt gaaaaaccat ccgcttgtcg aaccttttga attgctgact gaaatgtaca
     2461 gtctgccaaa atatgaagaa gtagatccaa caccttggat gacaccattt tatcttgtct
     2521 tcttcgggat gatggtcgca gatgttggtt atggtttgct gatgctcatt gggacaatac
     2581 tagctcaaaa actacttgtt ctaccacgcg ggatgaaacg tttcgtaaaa ttctttgaga
     2641 ttctctcgat accatcgatc atctggggat tgatctacag ttcatttttc ggacagtcat
     2701 taccaaaaga actgtttggc atccgcttgc ctttcccaat cctatctacg acggatgacg
     2761 tcaatacgat tttgatttta tccgtgattt tcggtctgat acagattttg gttggattat
     2821 ttgtggcagc aaaggaaaat atcaggagaa aagattactt gagtgctgtc agtgatggat
     2881 ttgcctggca agggatccta attgggatcg ttattgcgtt ggttggtgcc atgttgataa
     2941 aaaatacagc atttgtttac cttggcgctg ggctatcgat cattgctgcc ttatgtatct
     3001 tgatcgttcc gattatccaa acgccgtcta aagtcaaagg tgctgccaaa ggtgcgtata
     3061 atctttacgg actgaccggc tatatcggtg atttagtcag ttatacacgt ttgatggcat
     3121 tgggtatttc aggcggaagt attggagcgg cattcaatat gctagttgct ttcatgccgc
     3181 cagctgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt
     3241 ttttgacact gctaagtgcc tatgtccatg gcgcacgatt gcagtatgtc gaattttttg
     3301 ggaagtttta tacaggaggc ggacgtgcct ttgatccgct gaaaacggcg gaaaaatacg
     3361 tcaacatcaa tcacaagaaa aaaaacaata aataattgga ggatttttat ataatgatgg
     3421 attacttaat tagtcaaaat ggtggaatgg tatttgcggt attagcgatg gcaacagcga
     3481 cgattttttc agggatcggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag
     3541 cattgacaac aagtcaaccc gaaaaattcg ggcaggctct gattctgcaa ttgcttccag
     3601 gcacgcaagg tttgtatgga ttcgttattg cctttttgat ttttatcaac ttaggaaatg
     3661 acatgtcagt tgtacaagga ctgaactttc taggtgcttc tctgccgatt gcttttacag
     3721 gcttgttttc aggtattgcc caaggaaaag tcgcagcggc aggtattcaa attttagcga
     3781 aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa acatacgcga
     3841 ttttaggttt cgttatttcc ttcctattag tgttgaatgc ctaagcataa aggaggaaaa
     3901 gcagatgaac gccattgaaa aaatcatctc tcagatgaac gaagcagcag agcaggaaag
     3961 agcggcgtta gaacaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac
     4021 gcaagttgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac tcgaaaaaaa
     4081 ataccgtcag ctaaggaatc gccagcaagt agaagtacga caagaaaatt tgaatgcgaa
     4141 gcaggaattt ttaagacgat tgtttgcaga tgcggtaact gaaatggaaa attgggatga
     4201 atctgaacaa atccagttta tcaaaaatgc attgtattca ttacctttga caggaaaagt
     4261 agcattcatt gctggagaaa aatcagcagc ctatctttct cagacgttgt tggatgaatg
     4321 gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg
     4381 ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga
     4441 tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag
     4501 tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc
     4561 aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg
     4621 aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat
     4681 cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa
     4741 atcgtttgga tctatacgat gcgttatact ttccataatc tgaaggtttt gacaaaagct
     4801 gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa
     4861 accttgaagg atgccattca tacgcaagtt tcaaatgaac ttcctgaaag cattatggac
     4921 agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg
     4981 atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa
     5041 gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga
     5101 ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt
     5161 atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt
     5221 ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatattgat
     5281 ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa
     5341 acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc
     5401 aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaatcaga aacaatcaga
     5461 aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt
     5521 tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg
     5581 tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg
     5641 tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt
     5701 attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt
     5761 agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat
     5821 tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag
     5881 tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgcg
     5941 tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc
     6001 ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat
     6061 gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt
     6121 agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac
     6181 agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa
     6241 agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga
     6301 aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa
     6361 aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt
     6421 aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac
     6481 aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca
     6541 tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg
     6601 aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga
     6661 atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag tagcagctcg
     6721 agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga
     6781 cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg
     6841 acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc
     6901 ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag
     6961 tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca
     7021 aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag
     7081 acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa
     7141 gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat
     7201 tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc
     7261 agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca
     7321 aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa
     7381 gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag
     7441 tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc
     7501 ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac
     7561 gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg
     7621 tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag
     7681 tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag
     7741 caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat
     7801 ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg
     7861 gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta
     7921 acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta
     7981 tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg
     8041 gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg
     8101 attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg
     8161 aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga
     8221 atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg
     8281 cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca
     8341 actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag
     8401 gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc
     8461 gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa
     8521 ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag
     8581 agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa
     8641 aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc
     8701 tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat
     8761 ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat
     8821 atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg
     8881 aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca
     8941 aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca
     9001 tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa
     9061 aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc
     9121 tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg
     9181 cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt
     9241 tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg
     9301 atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg
     9361 atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg
     9421 aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat
     9481 tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg
     9541 aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg
     9601 actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa
     9661 gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg
     9721 cggcggccta cttaccctac cattttttag taatagtggg catgggacaa attttatcga
     9781 tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc
     9841 agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct
     9901 tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag
     9961 cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct
    10021 catgctttac atcctaaaat tggcctttgt gatccaattg attggtacgg ttgctttatc
    10081 ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc
    10141 aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca
    10201 gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt
    10261 catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa
    10321 aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac
    10381 agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt
    10441 ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag
    10501 ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac
    10561 ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt
    10621 gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg
    10681 ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc
    10741 ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc
    10801 tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa
    10861 attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc
    10921 tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat
    10981 gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa
    11041 taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac
    11101 tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca ataagatttg
    11161 attcctttat ttatcaatag gttataattc atattttttt attttttctt ttacgatact
    11221 tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa cgaatgcatc
    11281 tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt tagcaagaaa
    11341 gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc gatagtaagg
    11401 gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat tggctctttt
    11461 tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga ggatttgagt
    11521 gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg cttgttctat
    11581 cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag aacaaataaa
    11641 ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga aaggaggaat
    11701 gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa taacccatag
    11761 accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc
    11821 tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt ttctacattc
    11881 ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa
    11941 atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg ttgctattct
    12001 atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa caccggaaac
    12061 aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat ttgctgcagt
    12121 agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa caagatttgg
    12181 gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt caaaagaact
    12241 aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg tgatggactc
    12301 aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca aacctttctc
    12361 aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat aatcagctag
    12421 ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa tatcttgttc
    12481 taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt ctggtcgcca
    12541 agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt aaaattgtat
    12601 accatacaat tttaccattg attgtattga ttttagaagt taatcttttg tgaggaggga
    12661 attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt ttgaagaagc
    12721 gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc
    12781 tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc
    12841 ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc cggataatca
    12901 tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc aaaacggact
    12961 acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg ctgtatctgc
    13021 gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa ttggagctgg
    13081 tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca ctacagtgtt
    13141 tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta aaaaacagta
    13201 tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc cagcggaaat
    13261 catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg taagggaaga
    13321 cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa gtacatcgtt
    13381 gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag aaagcgggga
    13441 cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg gtacattagg
    13501 cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga cgattttcga
    13561 cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata acaaattaga
    13621 gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc agatgaggtg
    13681 ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga aggtacgatg
    13741 caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa gaaaaaccaa
    13801 gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg tcacgcgaca
    13861 ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac gattaaattt
    13921 tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta catggatttt
    13981 cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc gattggggct
    14041 aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga tgaaaaaacg
    14101 gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg aataggagtc
    14161 atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt tccaaaatta
    14221 acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc ttattgggca
    14281 gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg cggggatcta
    14341 gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt gttttctaaa
    14401 ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg gagagtggca
    14461 accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt
    14521 ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta aaatgcccca
    14581 tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga caaagtagta
    14641 agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccag cagctttatg
    14701 ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat agccgtctcc
    14761 tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt catccccagg
    14821 aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc ggctttcttc
    14881 aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca ctcgaacaca
    14941 tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc ataacaaaaa
    15001 gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata
    15061 gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg gtgaaaaaaa
    15121 gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa gagatcagca
    15181 gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg aaattgaagg
    15241 agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg gaattgtggc
    15301 agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt atcgtgcaac
    15361 ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta taggagaacc
    15421 aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa
    15481 acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa actgaaagaa
    15541 agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat tttttgattg
    15601 cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct tctttgacaa
    15661 gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg gataagtcgt
    15721 tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc tgtgcgagat
    15781 tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa tagggcgcac
    15841 ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta
    15901 aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt gtcatttctt
    15961 caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc
    16021 tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga tgactgccga
    16081 cacggcgtgc cattacttcg atctctagga aatcttcatg tgtcagtccg tgacgggcga
    16141 tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata acgtaatcat
    16201 tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag ggacggccag
    16261 tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct tttacttctt
    16321 ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg actagtttga
    16381 tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca ttacggatat
    16441 ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc aaggacggat
    16501 caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc gctaatgcag
    16561 caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc aaaatctcga
    16621 tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga tcgtagctag
    16681 aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac aaagtgagtt
    16741 cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt ggtttacgat
    16801 tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac cattgagtaa
    16861 agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta aagcttccgt
    16921 caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct tcttgataca
    16981 actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg gaaacaggat
    17041 ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc actgcatgca
    17101 tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt aatattcggg
    17161 taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc aaatagtcca
    17221 ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg gggtaagttt
    17281 tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta agaatctgat
    17341 acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga ttcgtatgga
    17401 agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac agtgccgcac
    17461 ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt tcttttgaaa
    17521 aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct aatgaatgag
    17581 taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc
    17641 gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga tgctggacat
    17701 ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt tgatatttat
    17761 aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct ctggatttgg
    17821 ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc ctgtaaatca
    17881 cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa
    17941 aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac tgcaggatag
    18001 ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat gacttgccca
    18061 cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc atttttgatc
    18121 cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata agttccagga
    18181 caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc
    18241 aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc actttggggg
    18301 aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc acctgaattt
    18361 ctgatcactg gagcgtaacc tgcatcttcc agtgtttgga tcccgtcact tagaaaagga
    18421 gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa atgtaaaaag
    18481 atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa aggggaaaag
    18541 tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca accggctcct
    18601 ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt gttttggaaa
    18661 aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact aggaacaatg
    18721 tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca ccgatttcaa
    18781 tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat tttgatttga
    18841 aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag attcaggagg
    18901 agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat gtacagctga
    18961 tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg ttagaaacat
    19021 cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa ctgcatgtta
    19081 gtttgaaaga ccgtcccaca gcagggaagg ttcgaatcga ttatgatctg ttcatgggac
    19141 cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata taaaagaaaa
    19201 gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag gacgtgtcct
    19261 gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg atcgaagtag
    19321 cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta gctaaccaga
    19381 tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca caattttata
    19441 ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg ggcttgcgtt
    19501 catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac gaagaggatg
    19561 aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag
    19621 acgcgatcga ttacaatgac gatgatgatg aagatgaaga aatcgctgct tataattctg
    19681 acttgcaaga aatcggagca gatgacccga cagatgatga agaagacttg ccaggaaata
    19741 tcgaagaaga tttaagtatc atagacgaag acgatgatga tgaagattat gaagaggaag
    19801 aattttctga ctctgaaaaa gaataaacat tattttctta cgagaggttg tgacagagac
    19861 gatcagccta tcagaataag gaggtgtgac acgatttttg tcacaccttc ttttttgtat
    19921 ataaaaatcc caagtaaata cataaaaaat aaaaaaattg aaacttttag cgcaccttgt
    19981 acgtcatatt aagtgaggca gaaaggagtg gagaatgatt tttactggat ataagaaaaa
    20041 acggcagatc gaaaaatacc gtcacctgct gaatggactc atcgaaactg attatcaaaa
    20101 aatgttccgt atcacgatga attacgtaca tgataaagaa gaggccttag atgtgatgca
    20161 agatagtttt cataaagccc tgaactattt tgaaaaagaa aaagagatcg agcattttag
    20221 tgcatggttt tatcggatat tgatccgcac ggcattagat agttggcgca gacaaaaaag
    20281 gacgcctgtg gacttatttg aaattcaaga tatcccccaa cagttacctt tagaaacttc
    20341 cgttgcagag ctccatatga ttttagataa aatagacagt ccggaaaaag agattctgat
    20401 cctctacttt tttgaaggat ttcgtttgaa agaaattgcg actatcttgg atatgaatga
    20461 aaacacagtt aaaacgaaaa tgtaccgctc attgaatgct ttacgtcaaa tcctagaatg
    20521 aaggagagaa gagtgccatg tattcccaaa aagaaaattt aaaaaaacta aagaaaactt
    20581 ataaagaaca agcaattccc aaagggctta aagaggagat ccgccagcgt tttattatcg
    20641 aagagcaatc atttataaaa gcaaagcgga gaaagagacg tctccaaggg ttcggagtat
    20701 ctttggctat cgtaatgatt acaacaagta gccttctttt taatagtcaa gttcgaagct
    20761 ttgcagaaga tttacccatc ttaggatcag tcattgaatt gatcttaggc gaacgcttta
    20821 ctgatcgatc tgaaaaaatc gatattcagg taccgaagat caatacacaa aatgagaaag
    20881 aaaataaaac aattcatgga ttaaatcaaa aatactttcg cgaaggacaa gcagattttg
    20941 aaaaagctga aaaagaatat ggaaattttg aaacggatca ttaccaagtt ttgggagatt
    21001 atcagaaaat cgtagacgat aaccggtttc tcgtgattga aaggcagatc acccaaacag
    21061 cagcagatag ccatgttgaa aaaagatacg acacaatcga caagaaaaac agtgtccagc
    21121 tctcacttcc acttttattc aaagatgaca cctatctatc tgttttgaca aaagaagtca
    21181 aacggcagat ggccgaacaa gtcaaggaag atccatctaa gtactattgg actgagcaag
    21241 atattcaaga agggacgata gaaaaaccaa cacttgttac gcctacacga agtttttatt
    21301 tgaacaaaga gcatcaatta gttttgactt tttctcaata cgaaattgct cctggttata
    21361 tgggtacacc agaatttgtc attcctaagt ccgtgacaaa aacgattttg gcatcggaag
    21421 attatttgga tcattagacg agaagtagtt gcgactagca gtttcaattt tcaagtaagt
    21481 acaagtaaat aataagaaga gaaaaagaga aagaagagat atagtgaaaa gaaattattt
    21541 agttatcagt attattgctt tatccgtgct tttgtttttc gaaattactt cggtggcctc
    21601 cttgatcacc gaagtaaaag gacaaacaca ctcttcggag cattcgtcac aatctttcaa
    21661 acaatcagaa caatcaacca ttaaaagcca atcaactgaa acagctaacg tcaaagaagc
    21721 cattcaagaa tcagcggaaa aggtacaaag tcagtcatca caatcaacac aagacagtac
    21781 aaaaacgagc cgagatccgg ctgtcaaaag cgtcgcaatc agctttgatg atggaccagg
    21841 agcgaccaca acaccgcagc ttctgcgaat cttaaaagaa aaaaacgtgc atgccacttt
    21901 tttcgtctta ggagaaaata cggcgcagca tcctgaaatc gtgaagcaga cagctgaagc
    21961 agggcacgag attggcaatc acacatacga tcatcagaat ctggccactc tttctgccca
    22021 gtcgatgacg gaggaagtga cgaaagcaga cactgagatc aaaaaagtca ctggcaaaac
    22081 ccccactttt gttcgtccgc cctatggttc tgtcacgaat gtaggagcaa cgatcatcca
    22141 gcgaccgatc atcgaatggt ctgttgattc agaagattgg aagacaagaa atcctgattt
    22201 gattttgcag aagatccaag caacagtata cgatggggca attattttat tccatgatat
    22261 ctatcctgaa acgattcgtg ccgttcctca agtgatcgat tatctgaaag agcaagggta
    22321 tcgcatcacg acagttgggg atttattagg gcatccaacg gctgtcgaga actattatgg
    22381 cagaaacgat catcggccag ttcagtaagt gagacaagta tctttaataa ataacacatt
    22441 ttaataagta gttgaacaca taactacctt acttgcaaag gcttaacaat atctatacag
    22501 gtacaacaaa aaactgtcca ccaattttct ttggcggaca gttttcactt aaaaataaag
    22561 aagcaactat tcgtaaatta tgctaaaata gcagtagaac gcgtcctgag ggaatcgaac
    22621 ccccgtctca agaaccggaa tcttacgtga tatccactac actaaggacg caagcactgg
    22681 gataattgta actaaacaga taaaaaatta caaggggatt ggacaaaaaa atgaaatttg
    22741 agcaaaattt ttcacaaaaa cagcagcaga cacagaaact agccatgacg caacagttgc
    22801 aacagtccat ccaagtttta caatataaca gtgaggagct gttggcattc gttgagaatc
    22861 aagcaatgga aaatccattg gtggaagttg ttgagccgga atggcagcca gattacataa
    22921 aagcttcttc atcttcttat gaaggagaag agacaaatta cctaaatcag attcctgata
    22981 caaaagggtc tttatttgat tcattgatcg aacaagtcca tttgaattac cgagatactt
    23041 ttttgaggaa aatcgttctt tatcttgtag aatacatcga tttgaacgga tttttgacga
    23101 ttagtttaga agaagccgga aaagagattg gagctacgcc tatccaaatg ctcgatgcgt
    23161 tgactctgat tcagcaatta gaaccagctg gagtaggtgc acgtagtttg caagaatgtc
    23221 tcatgcttca aacagaacga gatgattatg cgccggaatt agcttatatc gtgttggaag
    23281 aatgttttga ggaactggtg gagcgaaaat ggaaagaaat cgcacaacgt ttttctgttg
    23341 atctacatgc tgttcagcag atattcgact atttacaaac gttgagtcca agccctggcc
    23401 ggattttcga ccgtagcagc gaattgtaca ttattcctga tgtccgggta ttagtggacg
    23461 acgataaaaa tgtacaagtt atctcaaacc gaaaaaatca gccaaatata cgctttcaag
    23521 agagttactt caaacaaatg tcacaacaag cagataaaga aactgaaaat tatttaaaag
    23581 aacggaaaca ggaattcgaa tggttgaaga aaacgatcct acaacgtggg gatacaattt
    23641 tgcgggttgc tcaagtcatt gtttcccggc aaaaagcttt ctttatcgac aaggaacgtc
    23701 caatcaagcc tcttacgtta aaggaagttg caactgagat agatgtgcat gaatccacgg
    23761 tcagtcgtgc agtcaacggg aaatatttgg aaacagactt cggtgtattt gaattaaaaa
    23821 aatttttcac tacacgaatt cctacgaata gtacagaaca gacagaagac ctttcagcag
    23881 atacagcgaa gaaaaagctg caggaattag tcgatcaaga agacaaaaac aagccgttgt
    23941 ctgaccagaa attggttgaa ttgctgaaaa aagatgagat agcgatctca agacgaacag
    24001 tagcaaaata tcgtgattta ttaggaatcc cgagttcatc aaaaagaaaa cgttatgata
    24061 actaatcagt caaaagacta gaaaaagtat ttcacttttt ctagtctttt tagttgaaaa
    24121 acgagagcaa aactgttaga cttacagtcg tgaggttgag atttcccttt attgatgtga
    24181 aattcgaata gttaatttct ttttttatcg tatatgggtc gttttaagtc tacattggac
    24241 gtaaagtgtc caactaagga gatcgctatg ctggatgaaa taaaaatgat tgaatctgtt
    24301 gcaccagata ttatcgaagt gttgcaagaa cgctataaaa ttttacgaaa tatctattgg
    24361 atgcagccga ttggacgtcg aagtttatcg gaaagtatgg ggatcacaga acgagtattg
    24421 cgaacagaaa cagatctttt gaaaaatctg aatttgatcg acacctcaaa aagcggtatg
    24481 acgctgacaa ctaagggcga agaagtttat caaagccttg aaaacttcat ggatcagctt
    24541 ttaggtacgc atcaaaccga acaacagttg gcagaatatt ttggtaacca acgctgtatc
    24601 gttgtttcag gaaacagcga agagcagacc aaagtggctg atgcattcgg cgaagcgctg
    24661 agcgaagccc tagaccgact acttccagaa ggcgagaata tcatcgctgt gatgggagga
    24721 actactatgg ctgtggtagc cgaacaattg tctaatctgg aaaataagaa acggcataac
    24781 ctgtttgtcc ctgcaagagg cgggattggt gaagcgataa ctgtacaggc aaattctgtc
    24841 agtgcaagaa tggctgctaa agcaaacggt aaccaccgtg cactttatgt tccggaacaa
    24901 ttaagtctgg caacttacaa ttctttactc aatgagccat ctatccaaga agtcttaaac
    24961 ttgattagtg aagcgaattg tgttattcat agtattgggc gtgcattaca catggcagca
    25021 cgtcgtaaga tgactgaaaa agaattagtc atgctgaaac aagcaaatgc tgttgcggaa
    25081 tcttttggct actttttcaa tgaaaaaggt gaagtcgttt ataaggtccc tagaatcggc
    25141 atagaattgc aagatttgga aaaagtcccg atcatcatgg caatcgcggg cggaaaatca
    25201 aaagctaaag caattcgagc ttatatgaaa aatgcaccaa aacaaacgtg gctcatcact
    25261 gatgaagctg ctgcaaatga gattttaaaa ggggtaaccc tttaaaataa atattttttt
    25321 gattttcata aggaggaaat cttaaatgac agttaaagta ggtattaatg gttttggacg
    25381 tattggacgt ctagcattcc gtcgtatcca agatgtagat ggaatcgaag tagtagcaat
    25441 caacgacttg acagatgcta aaatgttggc acacttgtta aaatacgaca caactcaagg
    25501 acgtttcaac ggaacagttg aagttcatga aggttcattc aacgtaaacg gaaaagaagt
    25561 taaagtatta gctaaccgca acccagaaga attaccatgg ggcgaactag gcgtagatat
    25621 cgttctagaa tgtactggtt tcttcacttc taaatcagca gctgaaaaac atttaacagc
    25681 tggtgctaaa cgcgttgtta tctctgctcc tggcggaaac gatgttccaa caatcgttta
    25741 taacacaaac cacgaaacat taactggtaa agaaacagtt atctcaggtg cttcatgtac
    25801 tactaactgt ttagctccta tggctaaaac attgaacgac aaatttggtg ttgttgaagg
    25861 attaatgaca actatccacg cttacacagg tgaccaaatg actctagacg gacctcatcc
    25921 taaaggtgac ttccgccgtg cacgcgctgc tgctgcaaac atcgttccta actcaacagg
    25981 tgctgctaaa gctatcggtt tagtaattcc tgaattgaac ggtaaattag acggagctgc
    26041 tcaacgtgtt cctgtaccaa caggttcatt aacagaatta gtaacagttc ttgaaaaaga
    26101 agtaactgtt gacgaaatca atgcagcaat gaaagaagct tcaaacgaat cttacggata
    26161 caacacagac gaaatcgttt cttcagatat cgttggtatg acttacggtt cattatttga
    26221 tgctacacaa actaaagtaa tgacagttgg cgacaaacaa ttagttaaaa ctgttgcttg
    26281 gtacgacaac gaaatgtcat acactgcaca attagtacgt actttagaat acttcgctaa
    26341 cttataagat agacaaacgt acaagtgtaa attatagaca tgtacattta aaagcgggga
    26401 agcaatcgcg cttcttccgc tttttttgat aataagttta ttcaggagga aataaaatgg
    26461 ctaagaaaac agtgaaagat atcgatttaa aagacaaaaa agttcttgtc cgtgttgact
    26521 tcaacgttcc tttgaaagac ggcgtgatca ctgacgacac tcgtatcaaa gcagcattgc
    26581 caacaatcaa ctacgttcta gaacaaggcg gaaaagcaat ccttttctct caccttggac
    26641 gtgtaaaaac agaagaagat aaagaaggaa aatctttagc tccagttgca aaacgtctag
    26701 gcgaattatt aggtaaagaa gtaacattcg tacctgaaac tcgcggagaa caattagaag
    26761 aagcaatccg caacatgaat gacggcgacg ttgtcgtatt cgaaaataca cgatttgaag
    26821 atattgacgg taaaaaagaa agcaaaaatg atacagaact aggtaaatac tgggcttcat
    26881 taggcgatgt atttgttaac gatgctttcg gtacagctca ccgtgcacat gcttctaacg
    26941 taggtatcgc ttcaactggt atcccaacag ttgctggatt cttgatggaa aaagaaatca
    27001 agttcatcgg tgaagcagta gaagaaccaa aacgaccaat gattgcaatc cttggtggcg
    27061 caaaagtttc tgataaaatc ggcgtaatcg aaaacttgat tcctaaagca gataaaatct
    27121 tgatcggtgg cggaatgact tacacattct acgaagcaaa aggaatcaag atcggtaact
    27181 ctctagtaga agctgataaa gtggaactag caaaagaatt aatcgaaaaa gcaggcgaca
    27241 aacttgtctt gccaatcgac aatgtctgtg caccagaatt ttcaaatgat gttgaaacac
    27301 aagtaatcga aggagacatt cctgatggtt tgatggcttt agacatcggc ccagcatcag
    27361 taaaattatt tgctgataca ttacaaggtg caaaaacagt tgtttggaac ggacctatgg
    27421 gtgtatttga aatgagtaac tttgctaaag gtacaatcgg tgtttgtgaa gcaatcgcta
    27481 acttagaagg cgcaacaaca atcatcggtg gcggagactc agctgcagca gcagaacaat
    27541 taggatttgc tgacaaattc acacacatct caacaggtgg tggcgcaagc ttggaattgc
    27601 ttgaaggtaa agaattacca ggactagcag caatcaacga taaataaacg accaagtttg
    27661 ctcaattctg acgagtaatc gatcaaaagg tgaagggagt tttcatgact ccctagccat
    27721 ttttcaataa attaaggagt gtgttcctca atgcgtaaac ctattatcgc aggtaactgg
    27781 aaaatgaaca aaactgcatc agaagcaaaa gaatttgctg aagcagtaaa aaacaaaatc
    27841 ccttcaaatg acaaagttga ttcagtcatc ggatctcctg cattattctt acaagaatta
    27901 atgacatcag ctgaaggtac agaactaaaa atctcagcac aaaactgcta ctgggaaaac
    27961 tcaggtgcgt tcactggtga aacttcacca gcagctcttg ctgatctagg tgttcattat
    28021 gtgatcatcg gacattcaga acgtcgtgaa tatttccatg aaacagacga agacatcaat
    28081 aaaaaagcaa aagctatctt tgcaaatggt atgacaccaa tcctatgctg cggcgaatct
    28141 ttagaaacct atgaagcagg caaaactgct gaatggatcg aaggacaaat tacagctggt
    28201 ttagctgatc tttcagctga acaagtgtca aacatggtta ttgcttatga accaatctgg
    28261 gcaatcggta ctggtaaatc agctgacgca caaatcgctg acgaaatttg tggagttgta
    28321 cgtcaaacag ttgaaaaact ttatggaaaa gaagtatcag aagcagttcg tatccaatac
    28381 ggcggttctg taaaaccaga aaatattgct gaatacatgg caaaagaaaa tgttgacggt
    28441 gctttagtcg gtggcgcaag cttgcaagca gattcatttt tgtcattgtt agacgctgta
    28501 aaataattgt tgaaaatttg cgaaaaatgc aagaaatgtt tgcactaatg agcaaaatta
    28561 actaaaatcg tatatgaggg aaatccctta ggaaaaaaca aactcaaagg agagacaaaa
    28621 catgtcaatt attactgatg tttacgctcg cgagatctta gactcacgtg gtaacccaac
    28681 aatcgaagta gaagtataca cagaaagcgg agcttttggc cgcggaatgg ttccttcagg
    28741 tgcttcaact ggtgaatacg aagcagttga attacgtgac ggagacaaag ctcgttatgg
    28801 cggtaaaggt gtaactaaag ctgtcgacaa cgtaaacaac atcattgctg aagcaatcat
    28861 tggctacgat gttcgtgacc aaatggctat cgacaaagct atgatcgctt tagacggaac
    28921 tcctaacaaa ggtaaattag gtgcgaacgc tattttaggt gtttctatcg ctgttgcacg
    28981 tgcagctgct gactacctag aagtaccttt ataccactac ctaggcggat tcaacacaaa
    29041 agtattgcca acaccaatga tgaacatcat caacggcgga tcacatgctg ataactcaat
    29101 cgacttccaa gaattcatga tcatgcctgt tggcgctcca acattcaaag aagctctacg
    29161 tatgggtgct gaagtattcc acgcattagc ttcaatcttg aaagctcgcg gattagcaac
    29221 ttctgttggt gacgaaggtg gatttgctcc aaaccttggt tcaaacgaag aaggttttga
    29281 agtaatcatc gaagcaatcg aaaaagctgg ctatgtacct ggtaaagacg ttgttcttgc
    29341 tatggatgct gcttcttcag aattctacga caaagaaaaa ggtgtttacg tactagctga
    29401 ctcaggcgaa ggcgaaaaaa caactgacga aatgatcaaa ttctacgaag aattagtttc
    29461 taaatatcca atcat
//
LOCUS       DACEMO010000014        28546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEMO010000014 DACEMO010000000
VERSION     DACEMO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.014"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            227..715
                     /gene="purE"
                     /locus_tag="IW728_000462"
     CDS             227..715
                     /gene="purE"
                     /locus_tag="IW728_000462"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1023843.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            699..1832
                     /gene="purK"
                     /locus_tag="IW728_000463"
     CDS             699..1832
                     /gene="purK"
                     /locus_tag="IW728_000463"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1023844.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1845..3140
                     /gene="purB"
                     /locus_tag="IW728_000464"
     CDS             1845..3140
                     /gene="purB"
                     /locus_tag="IW728_000464"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1023845.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3320..3697)
                     /locus_tag="IW728_000465"
     CDS             complement(3320..3697)
                     /locus_tag="IW728_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023846.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            3953..4681
                     /locus_tag="IW728_000466"
     CDS             3953..4681
                     /locus_tag="IW728_000466"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1023847.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            4681..4935
                     /gene="purS"
                     /locus_tag="IW728_000467"
     CDS             4681..4935
                     /gene="purS"
                     /locus_tag="IW728_000467"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1023848.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            4937..5608
                     /gene="purQ"
                     /locus_tag="IW728_000468"
     CDS             4937..5608
                     /gene="purQ"
                     /locus_tag="IW728_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1023849.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            5609..7831
                     /gene="purL"
                     /locus_tag="IW728_000469"
     CDS             5609..7831
                     /gene="purL"
                     /locus_tag="IW728_000469"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1023850.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            7816..9255
                     /locus_tag="IW728_000470"
     CDS             7816..9255
                     /locus_tag="IW728_000470"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1023851.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            9287..10330
                     /locus_tag="IW728_000471"
     CDS             9287..10330
                     /locus_tag="IW728_000471"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1023852.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            10327..10905
                     /gene="purN"
                     /locus_tag="IW728_000472"
     CDS             10327..10905
                     /gene="purN"
                     /locus_tag="IW728_000472"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1023853.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            10902..12437
                     /gene="purH"
                     /locus_tag="IW728_000473"
     CDS             10902..12437
                     /gene="purH"
                     /locus_tag="IW728_000473"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1023854.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            12453..13727
                     /gene="purD"
                     /locus_tag="IW728_000474"
     CDS             12453..13727
                     /gene="purD"
                     /locus_tag="IW728_000474"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1023855.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            13849..14763
                     /locus_tag="IW728_000475"
     CDS             13849..14763
                     /locus_tag="IW728_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1023856.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(14896..16461)
                     /locus_tag="IW728_000476"
     CDS             complement(14896..16461)
                     /locus_tag="IW728_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1023857.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(16454..17905)
                     /locus_tag="IW728_000477"
     CDS             complement(16454..17905)
                     /locus_tag="IW728_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1023858.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(17919..18935)
                     /locus_tag="IW728_000478"
     CDS             complement(17919..18935)
                     /locus_tag="IW728_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1023859.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            19155..20243
                     /locus_tag="IW728_000479"
     CDS             19155..20243
                     /locus_tag="IW728_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1023860.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            20297..21823
                     /locus_tag="IW728_000480"
     CDS             20297..21823
                     /locus_tag="IW728_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1023861.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            21825..23030
                     /locus_tag="IW728_000481"
     CDS             21825..23030
                     /locus_tag="IW728_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1023862.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            23287..24264
                     /gene="guaC"
                     /locus_tag="IW728_000482"
     CDS             23287..24264
                     /gene="guaC"
                     /locus_tag="IW728_000482"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1023863.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            24528..25952
                     /locus_tag="IW728_000483"
     CDS             24528..25952
                     /locus_tag="IW728_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1023864.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            26413..27522
                     /gene="pdhA"
                     /locus_tag="IW728_000484"
     CDS             26413..27522
                     /gene="pdhA"
                     /locus_tag="IW728_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1023865.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            27525..28502
                     /locus_tag="IW728_000485"
     CDS             27525..28502
                     /locus_tag="IW728_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1023866.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         9222 a         4877 c         6446 g         8001 t
ORIGIN      
        1 agcttctcat gtttgttgaa tattgttcct tgtgaccgag gagttggcag gagaacaccg
       61 tttatccata aaaaggaatg tgacctaact tttgtcacat tccttttttt tatctttaat
      121 aaacacgaac aataaaatgt ttttctttgt taacattcga aatttctttg acatcaaaaa
      181 aagtgattgt taaaatatga atgtagaaaa actgggagga aaaacaatga cagcaaaaat
      241 ctctgtgatt atggggagta cttcagattg ggaaacaatg aaagaagcat gtcaaatatt
      301 ggatgaattc ggtgtgtctt atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt
      361 gatgtttgaa tatgcacaaa acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc
      421 agggggagcc gcgcatctgc cgggtatggt cgcatcaaaa acaactttgc cggttatcgg
      481 tgtaccggtc caatcacgaa cattaaatgg tttggattcg cttctttcga ttgtccaaat
      541 gcctggagga gtacctgtag ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg
      601 actacttgcg atacagatgc tttccatgta tgatttagaa ttggaggcta aattggctga
      661 aagacgttca atgttggcta aaactgttat agaaagtagt gatcaacttg gttaaacctt
      721 tgttgcctgg ttcaacgatc gggatcgtcg gtggcggtca attaggtcgt atgatggcga
      781 ttagtgcaaa agaaatgggc tttaaagtag gtgtgctgga tcctgtgagc gattgtccag
      841 ccgcacaagt tgcagattgg cacattgaaa gtgtctatga tgacaccttt gcgctgaaag
      901 agcttgccag aagaacagat gtcattacct atgaattcga aaacgtcagt atagaggcgt
      961 taaatgcgat tttacccatg tcatttattc ctcaagggac agacttgctt gcgattacgc
     1021 aagaccgcct gttagaaaaa tcttttttgg aaacgaataa catcgtgatt gctccttatg
     1081 caacgatcgt cagtccgacg gatatccaag atgcgattga cgggatcggc tatccttgtg
     1141 ttttgaaaac cactcgaggc ggctatgatg gcaaggggca gtatgttttg aaaagtcggg
     1201 cggatctagc tcctgccatg gaccttttaa gagaaggaac ctgtgtttta gaagcatgga
     1261 tcccgtttga aaaagagatt tcgatcatgg tggcaggaaa tggtcaagga gatttcacga
     1321 cttttccggt cgtggaaaac atccaccaca acaatattct tcatgaaacg attgctccag
     1381 ccgcgattga tcaggatgtg atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag
     1441 cagtgagcct ttcaggtgtt ttaggggtag aaatgttttt aacaaaaaca ggcgggctat
     1501 atgtgaatga attagctcct cgtcctcaca attctggaca ttattctatt gaagcttgtt
     1561 ctatgagtca gttcgatgcc catattcgag gtatttgtgg ttggccatta ggagaaaaag
     1621 tacgtttgat aagcaatgca gtaatggtca atgttttggg agaacaactg ctggaaagct
     1681 atcgcttgat tgagaataag ccagactggc aattccattt ctatggaaaa aaacaagcaa
     1741 aaaaaggtcg caagatggga catatcacta tcttgacaga agacatccag caaacgctgg
     1801 aagaaatcaa acaaacgaat atttgggatt agaggagaaa aaacatgtta gaacgttata
     1861 cacgccctga aatggggaat atctggacgg aagagaacaa ataccgtgcg tggctcgaag
     1921 tcgagatttt agcagatgaa gcttgggcag aacttggtga aatcccgcaa gaagatgtga
     1981 aaaaaattcg tgagcatgca tcatttgata tcgatcggat cttggaaatc gaaaaattaa
     2041 cacgccatga tgtcgttgcg tttacacgag cggtttctga aacattggga gaagaaagaa
     2101 aatgggtcca ttatggtcta acaagtacgg atgtcgtaga tactgcttat ggctatctca
     2161 tcaagcaagc aaatgatatt ttacgggaag acttgaaacg ttttacggaa atcattggcg
     2221 aaaaagcaaa agaacacaaa tatacagtga tgatgggtcg gacacacggt gtccatgctg
     2281 aaccaactac ttttggattg aaacttgcca catggtattc agaaatgaaa cggaacatcg
     2341 aacgatttga acatgcggca aaaggagtag aagcaggaaa aatctcagga gcagtgggaa
     2401 cgttcgcaaa tatcccgcca tttattgaag agtatgtatg taaacaattg gggatccgtc
     2461 cacaagaaat ttctactcaa gttctccctc gggatctaca tgctgaatat ttctcagcga
     2521 tggcgcttat tgctacaagt attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat
     2581 ccgaaacacg tgaagtagaa gaattttttg cgaaaggtca aaaaggttct tctgctatgc
     2641 cgcacaaaag gaatccgatc ggttcagaaa atatggctgg tctagccaga gtggtgcgcg
     2701 gacacatggt cactgcctat gaaaatgtca cattatggca tgaaagagat atttctcatt
     2761 cttcagcaga acgaatcatc ttgcctgata ccacgatttt attgaattac atgttaaatc
     2821 gttttggcaa tatcgttaaa aatctaacgg tctttcctga aaacatgaag cggaatatgt
     2881 ctgcaacatt tggtttgatt tatagccagc gcgttatgct gaaattgatc gataagggaa
     2941 tgacaagaga agaagcttat gatcttgttc agccaaaaac tgcgtacgct tgggacaacc
     3001 aagtgcagtt ccgcccgctg ttagaagaag atccaaccat cacatcggta ttatctaaag
     3061 aagaaataga cgatgccttt gattatcatc atcatttgaa aaacgttgac gtgatattca
     3121 aacgagtagg tttggaataa agaagagtga gttgcaaaat tcaattaaaa tagaatagca
     3181 aaaataacca aaaaagaagc agatccgtta gcggttctgc ttcttttagt ttattatatt
     3241 ttcttatcaa taataggaac ctgattttaa aagcggctac ctctttaccg tgcctcttta
     3301 catacaaaaa atagaggaat taagcgtaaa tggcttggct attataacta aaatgctggt
     3361 tatggagatg ttgcagcaag ttttcgagaa aagcgtattc catctcttgt ccattgaata
     3421 agatctggat acgataatga tttttgaaac gcttctgttc atgaatctga ctgattgaaa
     3481 gttcgccttt gtgttctgtc tgataaagaa aagtcctttt acggcattca gcacagcgct
     3541 tggttgctcg gcggattttt cggaatgtat aaaaactagt atctttattg cataaataaa
     3601 cgtctttgat cgacagcaaa tcattatata gacaggaacg ttcttcagct gttaattctg
     3661 ataaaaaaag ctgaccttgt tttattgcat cttccatcgg caagtgcctc ctcgaaccca
     3721 ttatccaaat ttttagttaa ttctaatata ccatcattaa gtataaatgt aaacgcttgt
     3781 tgatggtata agattttcgt ttctgataaa acacgaacat taaaagaatc taatgcctta
     3841 aatgtacata tttttgttga tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac
     3901 gaacaaaaaa cgatgtattt gcttttaatg taaataaaaa ggagagacgt acatggagaa
     3961 gaaagaactt ttgtatgaag gaaaagccaa aaaaatatat cttacttctg atgagaactt
     4021 gatttggata gaatatttag atcaagcaac tgcgttgaat ggcgcgagga aagaccagat
     4081 aagtggaaaa ggagaattga ataatcaaat cacaagtgca attttccgtt atctgactca
     4141 aagaggaata aaaaatcatt ttattgaaca attgtctaaa accgaacaat tagcaaaaaa
     4201 agtacaaatt atccctctgg aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa
     4261 acgtctaggt gttcccgaag gtaaagaatt catcaaacca atcattgaat tctactataa
     4321 ggatgaccag ttggatgatc catttatcaa tgaagagcat atctatttct tacaacttgc
     4381 aaagccggaa gaaatcagtg agctgaaagc ccaagcgttg gctattaatg aaattttgac
     4441 cgaattattt gctaaggtcg gtttggtttt agtagatttc aagctagaat ttggccggtc
     4501 gaatggacaa attttgcttg cggatgaagt aacaccggat acttgccgat tatgggatga
     4561 acgtacgaat gaacatatgg acaaagacgt ttaccgcaga gatctaggtg atttgatccc
     4621 agtctatcaa gaagtatata accgtctatc ggaactaact gaagaaaagg agcgagtatg
     4681 atgtatttcg taaaagtata tgtaacctat aaagattcag tgttagatcc tcaaggggag
     4741 gcagtaaaaa aagcagtcca tcgtatggga tatgaaacaa tcgaagatat ccgaatagga
     4801 aaatactttg agatacaact tgctgatgca gaagaaccaa tcgatacagt gattgaaacg
     4861 atttgtgaca agctgcttgc aaatgtaaat atggaaacat accgatatga aatcaaaaaa
     4921 atggaggaag tataacatga aatttgcggt catcgttttt ccaggttcaa attgtgacgc
     4981 agatatgtta tgggcagttc gcgagatcat gggcgctgat gccgaatttg tacggcatga
     5041 tgagtcctct ttagaaggat ttgatggggt tttactgccg ggaggattct cctatggtga
     5101 ctatttgaga tgcggtgcga ttgctcggtt ttctacaatc atggaagaag tgattcgttt
     5161 tgctgaagaa gggaaaccag tattcggtac atgcaatgga ttccagatat tgacagaagc
     5221 aggacttttg ccaggtgtac tgagacgaaa tgagtctttg cattttattt gcaaaactgt
     5281 accattgaaa gtcaacccca agacggcctt tacttcagag tatgaagccg acgaaataat
     5341 ctatcttcca gttgctcatg gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga
     5401 actaaaaaaa aataatcaga ttgcctttac ttataatgac aacatcaacg gcagcgtgga
     5461 aaacattgca gggattacga acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga
     5521 acgtgcagta gaagacttgc taggatcaga agatgggaaa cggttctttg cttccatttt
     5581 aaaaaacttc ggaaaggtga ccgtatagat gaaaacattg aaagaaccga cagctgaaga
     5641 gatcaagaaa aaaagaatct atgccgaatg gggattgact gatgaagaat atcgaatgat
     5701 cgaagaagat atcttaggaa gaatgcctaa ttatacggaa actggactgt tttctgttat
     5761 gtggagcgaa cattgttcct ataaaaattc taaaccggtt ctacgcaaat ttcctactag
     5821 tggacctcat gtgttgcaag gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg
     5881 actagcagtt gtcttcaaag cagaaagtca caatcatcct tcagccgtgg agccttatga
     5941 aggagctgcg acaggagttg gcggaatcat cagagatatt ttcagcatgg gggctcgtcc
     6001 aattgcgttg ctagattcat tgaggttcgg cgaattagat aatccaagaa cgaaatattt
     6061 actagaagaa gtagttgcag gtatcggcgg atacggcaat tgtatcggca taccgactgt
     6121 aggcggcgaa attgcatttg atccttgtta tgaaggaaac ccattagtca atgcaatgtg
     6181 cgtaggtttg attgaacaca aacatatcca gaaagggcaa gccgctggcg tcggtaattc
     6241 gatcatgtat gtaggtgcaa aaacaggtcg cgacggtatc cacggagcaa cttttgcctc
     6301 agaagaattt aatcaagaag aagaacagca acgttctgct gtacaagtag gcgatccttt
     6361 tatggaaaag ctgctgcttg aagcgtgttt ggaattaatc ttgcatcatt cggattctct
     6421 aataggtata caggatatgg gagcagcagg acttgtttca tctagtgcgg aaatggcttc
     6481 aaaagctggc tcaggactta tattgacatt agatgaagtc ccacaaagag aaactgggat
     6541 gacaccttat gaaatgatgt tatcggaatc acaagaacgg atgttgattt gtgttaaatc
     6601 aggagaggaa gaaaaaatcc aagaactatt tcagaagtat gatctagatg cagtcactat
     6661 cggtaaagta acagacgacg ggcaatatcg gctatatcat caaggagaag aagtagcaaa
     6721 tctgccagtc gatgcattag ctgaagatgc accagtttat cataaagaaa tgaaagaacc
     6781 agctcgaatc gtagaatttc agcagttagc tccttatcag ccagttatcg aagaacctgg
     6841 acaagtattg cttgatctgc tgcagcaacc gacgattgct tcaaaacgtt caatctacga
     6901 aacttatgat tcacaagtac aaaccaatac agttgtccgt ccaggaagcg atgctgctgt
     6961 gatgagggtt agagggacga ataaagcgtt agctatgacc acagactgca acgcgcgcta
     7021 tctgtatttg gatccaaaaa tcggaggaca aatagcagtg gcagaagctg ctagaaatat
     7081 tattgctagc ggtggtaagc ccttggcgat caccgactgc ctgaactatg gttctcctga
     7141 taaaccagaa gttttttggg aattatcgac ttcagctgac ggaattgctg ctgcgtgtga
     7201 aacgttagga actcctgtta tttcaggaaa tgtctcttta tataatgaaa cggatggtca
     7261 agcaatctat ccaacaccaa tgattggtat ggtcggattg attgaggatc ataaacatat
     7321 cactactcag gaattcaaaa aatcagggga tctaatctat atattaggta agacgtttgc
     7381 agattttgat ggcagtgaat tacaaaagat gcagcttgga agaatcgaag gtgtgatccg
     7441 aaactttgat ctatccattg aaaagagaaa ccaagagtta gtattgacag ccatccaaaa
     7501 cggattgatt gaaagtgctc atgactgttc agaaggtggc ttggcaattg cactggcaga
     7561 atcagctttc aaacatcagc tagggatatc tgtacagttt gaattgtcct ctgctcaatt
     7621 atttgcagaa actcaatcaa gatttgtgtt gacagtcgca ccggaaaata agacacgatt
     7681 cgaagaaatg atgggagatg ctgctgtttt agcaggtaag gtgacggatg aagcgataat
     7741 cgaaatttca gcaactgacg gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg
     7801 ggaggatgca atcgtatgtc ttatgaagtg aagagtttaa atgaagaatg cgggatcttc
     7861 ggtgtttggg gtcatccaga cgccgctagt gtggcatact tcgggctcca tagtcttcag
     7921 catcgaggac aagaaggtgc aggtatcgtg tccaatgatc gaggaaagct taaggggcat
     7981 cgagatttgg ggctgctttc tgaggtattc aaagatcaaa gaaaattgca gcagttaaca
     8041 ggagaagctg caatcggaca tgtacgttat gcaacagcag gcaatggaag tgtggacaat
     8101 attcagcctt ttctattcaa atttcatgat cagtcgatcg gcttagcgca taatggtaac
     8161 ttgatcaatg ctcgttccct tagaaaagag ttagaggaaa atggggcgat cttccattct
     8221 aactccgata cggagatatt gatgcatttg atccgacgaa gtgaagaaac agatttttta
     8281 aataaattaa aaagtgcttt gaatgaagtc aaaggtggct ttgcctattt attaatgaca
     8341 gaaacggcaa tgattgccgc tttggaccca aatggttttc gtccattagc aatcggacaa
     8401 atgaaaaacg gagcatacgt gattgcctcg gaaacttgtg cattagaagt tattggggca
     8461 cgttttataa gagatgtcca gcctggtgag atagtgatca tcaatgacga aggaatccag
     8521 attgattgct ttacgaagga cacgcagctt tctatttgtt cgatggaata tatctatttt
     8581 gccagaccag attcaaacat cgcagggatc aatgtgcata ctgcacggaa aaatatggga
     8641 cggaatttag cgaaagaatc gccagtaaaa gcagatatgg tcgttggcgt acctaattct
     8701 tctttgtcag ccgcaagcgg ttatgcggaa gctagtggaa tcccttatga aatcggatta
     8761 gtaaaaaatc aatacattgc ccgaacattt atccagccaa cgcctgagct aagagagcaa
     8821 ggtgttcgaa tgaaattatc tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg
     8881 gtcgatgatt caattgttcg gggtacgaca agcagacgga tcgtccatct tttgaaagaa
     8941 gcaggggcaa aagaagtcca tgtgaggatt gcttctcctc ctttgaaata tccatgtttt
     9001 tacgggattg atattcaaac gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa
     9061 atcaaagaat gtatcggtgc agattcttta ggttttttaa gtgaagaggg tctgatcgaa
     9121 tcgatcggtc tggaaaaaga tgctccgtat tcaggattgt gtatggctta tttcaacgga
     9181 gattatccga cgccgttata tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa
     9241 gtatcgttct tttaaaagca gaccaagaag ggagtatatg aattcaatgg caaatgcata
     9301 cgcaaaagct ggcgtagatg tcgaagctgg atatgaagta gtagaacgga tcaagaagca
     9361 tgtcaaaaaa acggaacgtt taggtatgat gggcgctata ggaggattcg gcggttgttt
     9421 tgatttatcg gtctttcaat taaaagagcc agtccttgtc tcggggaccg atggcgtggg
     9481 gacgaaatta gcagtggcaa tcgaagcaga caaacacgat acaatcggga tcgactgcgt
     9541 agcgatgtgt gtcaatgata tcttagcaca aggcgcagag ccgttatatt tcttagacta
     9601 cttagcaacg gggaaaaatg atccgttacg tttggaaaat gtagtaaaag gtgtagcaga
     9661 tggatgtgtt caaggaaata tggcgctgat tggcggagaa acagctgaaa tgcctggtat
     9721 gtactcaggt aatgattatg atctagccgg atttgctgta gggatagctg aaaaatctca
     9781 attattgaca ggagaaacga tcaacccagg agatgtgttg cttggaatag catctagcgg
     9841 gattcattca aatgggtact ctcttgttcg caaaattttc tttgagcagc agcagttttc
     9901 tttatcatca aaactgccag aattgaaaaa aacacttggc gaagaattgc ttgagccaac
     9961 gaaaatctat gtttcttcct tgcttccgct gatcaaagaa aagaaaataa aaggagctgc
    10021 acatatcact ggtggagggt ttgtagaaaa tatcccgcga atgttaccag aatctatggc
    10081 tgcacaaatc aaacttggct catggccgat tcttccaata ttcggctgtt tacaaaaata
    10141 tggtgaaatt cctgagatgg agatgtatga aatcttcaat atgggcatcg gtatggtcct
    10201 ggcagtcgaa gaatcgaaag taaaagaaat agtacacgaa attgagcaag ctggcgaaaa
    10261 agtttatgaa atcgggaaag tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga
    10321 agaacgatga gaatcgctgt ttttgcttct ggaaacggaa gcaactttca agcgctagcc
    10381 gattatctat cgaaaaaagg attggaatct tcgattgact ggttgttttg tgatcagcct
    10441 gaagcatatg ttttgaaacg tgcaactgcg ttgagtgtac cggcagattg tttttcacct
    10501 aaagaatttg attcaaaaaa agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa
    10561 aagatcgatt tgatcgtatt agctggatat atgcgaatca ttggaccggt attgctgaaa
    10621 aattatgata aaagaatcat caatatccat ccgtctcttt tacctgcctt ccctggtttg
    10681 catggcatac gtgatgcctt tgaagctgga gtaaaagaaa cgggtgtcac aattcattat
    10741 atcgaccaag gtgtagatac tggaccaatc atccggcagg aaaaagtgag aattgaacag
    10801 gaggacacgt tcgattcttt agaagaaaaa atccatagag tagagcatcg aatctatcca
    10861 gaagtgatta gtgaaattat tgaaaatgga ggctatctga aatgacaaga gcattgatca
    10921 gtgtatcaga taaaactggg attgtagcat tcgcccaagg tttgattgaa caaggagtag
    10981 aaatcatttc aacaggagga acaaagcatg cgttggataa agctgggatc aaaacgcttt
    11041 cgatcgaaga agtgacaggg tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc
    11101 ctaaaatcca tggtggtctt ttaggaagac gcgatctccc tagccatatg gaggcaatgg
    11161 aaacggaaaa tatccaacca atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa
    11221 cgattatgaa accagatgtc gaaatagcag aagctattga aaacattgat atcggtggtc
    11281 ctagtatgct ccgtagtgca gcaaaaaacc atgaatttgt cacctctgtt gttgatccat
    11341 ctgactatga agaagtactt tcagaattac gtcagtatgg gcatacttca ctttctttca
    11401 gacaaaaact agcggcaaaa gtctttcgtc atactgcggc atatgatgca ctgatcgctc
    11461 aatacttaac ggattgggtg gaagaaaaag aaccggaaaa attgacactt acgtatgaac
    11521 gaaaacaaac gcttcgttac ggtgaaaata gccatcaagc cgctgccttt tatcagtccg
    11581 ctcttctgga aaaatattcg atcgcagcag caaaacaact ccatgggaaa gaactttctt
    11641 ataataatat ccgagatgca gatgctgcgc tacggatcat gaaggaatat gacgagccaa
    11701 cggtagtcgc cttgaaacac atgaatcctt gtggtattgg gacagcatct acgattttag
    11761 aagcgtggga agcagcgtat gaagcagatc ctgtttcaat ctttggtggg atcatcgttt
    11821 tgaatcgaga agtagatctg ccaacagcag aacagatgca taagctgttt ctagaaatca
    11881 tcattgcccc ttcttataca caagaagctt tggaattact gcaaaaaaag aaaaacatcc
    11941 gattactgac agtagacttt gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag
    12001 tcttaggtgg acttcttgta caagatcaag atctggcaat agaaaatgaa gaagagtgga
    12061 aagtcgtgac aaaacgtaag ccgactccag cagaagcaaa agcattagtg tttgcttgga
    12121 gagctgtcaa acatgtgaaa tctaatgcga tcgtactggc aaacgaacgt cagacagtag
    12181 gaatcggagc cgggcagatg aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc
    12241 aagagaaaat ggaaggtgca gttttagcta gcgatgctta ttttccaatg gatgattcgg
    12301 ttgaatatgc agccaaacat ggtataaaag caatcattca gccaggagga agcatcaaag
    12361 accaagattc tattgatatg gcgaataaat acgatctagc aatggtcttt actgatgttc
    12421 ggcattttag acattagaga ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg
    12481 gtcgtgaaca tgcaataagt aaaaaacttt tagccagtcc gaaggtggat acggtgtatt
    12541 gtgcgccagg aaatccgggg atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc
    12601 gagatcattc agcgttaatc cattttgtta agaaagaggc tattgattgg agtatcgttg
    12661 gtcctgaagt tcctttgtta aatgggatcg tcgatgattt tatggcggaa ggattgaaaa
    12721 tcttcggtcc gacaaaagca gcagctctga tcgaaggttc gaaaagtttt gctaaacaat
    12781 taatggatac ttatcaaatc ccgacagcta aatccagaag ttttgaatgt tacgaaaaag
    12841 cagcagcgta tatcaaagaa aatggtgcgc caatcgtagt gaaagcggac ggcttagcag
    12901 ctgggaaagg tgtagtcgtg gctaccacag tgaaggaagc gctggatgcg gcagaacaaa
    12961 tgttgaacca gcatcgattt ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg
    13021 caggagaaga attctccctt ttggcgtttg tccgtaatac caaggtgtat ccgatggtca
    13081 tttcgcaaga tcataagcgg gcatttgata acgataaagg gccaaataca ggcggaatgg
    13141 gtgcttattc accagtaccg caaatttcaa aaaagatgat tgaaaaagct gtggaagaag
    13201 ttttaaaacc agcggctttg ggaatgaaag aactaggacg ctctttcacg ggtgttttat
    13261 atgcgggttt gattgctacg aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg
    13321 gtgatcctga aacgcaagtc gttctttctc ggttgaagag tgactttgcg caagtaatcg
    13381 atgatttatt agagaatcgt acagtagagt taaagtggca gcaagaagga tacaatgtag
    13441 gcgttgtggt tgcagctgcc ggttaccccg aagaatatga gacaggaatg gttctaccag
    13501 attttctgga agaagagctt tctgtttact atgctggagt ttcctcaaat aacggcaaac
    13561 tagttggagc aggaggacgt ctttatttag tagaagcata cggtgagacg atcaaagaag
    13621 cacagaaaaa aatttacacc gcgttaatgg ataaaacgac agatggaaca ttttatcgta
    13681 cagatattgg agaaaaagct gccaatagcg aactgatcgt gaagtaaatc agctgcttcc
    13741 atttataaaa aatacaacaa acataatcgg aagcgccgat tttagtgaag ccttttcata
    13801 aagagtgtgg tacacttttg ttgtgtatat aaataaggag gagttcagat gaaaaaaatt
    13861 caattaggta cttctgatca gaaagcagct gcagtgatct taggctgtat gcgtatcaac
    13921 aatgcggaag atccagtaaa agtgattgag acagcttatg aaaacggcat tgattttttt
    13981 gaccatgcag atatttatgg aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca
    14041 aaaacatcaa tcaaacgaga agatcttttt attcaaacaa aatgtggaat cgttccaggt
    14101 gtgatgtttg atttttctaa aaaacatatc attgaatctg tagaaaaaag tttgacccgt
    14161 ctgaaaatgg attatgtgga tgctttactt cttcatcgtc cagatacatt ggtagaacca
    14221 gaggaagtag ctgaagcgtt tgatgaattg gaacggtcag gaaaagttcg ttattttggc
    14281 gtcagcaatc aaaaaccaat gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta
    14341 gtagctaatc agctgcaatt tggcttgaaa catactggaa tgatcgatca agggattcat
    14401 gtcaatatga cagatgaagc aagtatcgat catgacggta gtgtacttga ttattcccga
    14461 ttgcatgata tgacgatcca agcatggtca ccataccagt atggattttt tgaaggtgta
    14521 tttatcggga acgaaaaatt ccctgaatta aaccaaacat tagaagaact agcagctaaa
    14581 tatcaaacga caccaactgg cttggcttct gcttggattt tacgccatcc agccaatatg
    14641 caagtgatcg ccggtacgat gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa
    14701 attaatatgt ctcgggaaga ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct
    14761 taaagattgt cataagcagg tcaaaagggc tataaattaa aaagacagaa taacttgaaa
    14821 gctgttttca gcttcgagat attctgtctt tttttattgc aaatggaaag agtatcattg
    14881 tacggatttc gtttattagt tatttttgtt tttaaggaaa agctgtgtcc gatattcttt
    14941 tggagaaatc ccttgttttt tcttaaataa ataactgaaa tagttagggt cattataacc
    15001 aatttcaaaa gcaatatctg aaatacgata gtctgattgc tgcaataatt tttttgctaa
    15061 ttcgattctt tgttctgtca gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat
    15121 cgtactgaaa tgagagggac ttaatgcaac ttgttgagcg acccaagtta aggacatatc
    15181 tggatcggta aaattttctt tgataaaatc caatgcatgg ttcaaaattg gctgatattt
    15241 gttccttgaa ggctgtgagg gcagagcagt taagaaagaa atgaacatat aaactgtcga
    15301 acggtaaagc gagtcgtcgg cgctgattgc taagcgttga ctggtggatg tgcaatgaac
    15361 catttcttgc gggattttct tttctttcag gcagacatgc tggagtagct tagtcaagac
    15421 aaataaacga tataatcgtg ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat
    15481 gtacttagat acttcgtttt tcgataatga agacaaatca agtgagaggt caaagtaaaa
    15541 gacagaatca actggaagtt tcgcttcttg aatcaaatcc tcatagctga taatttgttc
    15601 tttaagggaa ctggcggttt caagtatcat ctgcgtgatc tcaaaagatg caggtaattc
    15661 gctgattctt tctgcgacag gaccaatggc gacagaaaaa ttattttttt gtcccttttc
    15721 taattcatgg accaacgttt gggctacctg ataactggtc tcgatcacat tttcttcaag
    15781 agaatcaact attaaaaatt tgatgaatcg agaggaaaca gcagagaaga tcacttgttg
    15841 attctcccta aataaaaact tcaaataatc actgagatga gcgtaatctt caaagtctgt
    15901 actgacttgg tttgctgcta ataatacggt catttttttc cctgctaaag agcgattgaa
    15961 agattcagca agtttcaatg tttcactgat cgtaagtgag cctttgaaca aaccgttcaa
    16021 gaaatgattt tttttcaatt cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg
    16081 caaatctaaa gcagttactg ctttttctat tgcttgtttc aattcgctac ttttaatcgg
    16141 tttaagcaga taatcatctg cctgtacctg gattgctcct ttcatataat cgaaatcgtc
    16201 gaatccacta ataaatatga gacgagtcca aggaagtagt ttccttgctt cttttgaaaa
    16261 agtcaacccg tccatgaaag gcatttggat atcagtcaga atgatgtcgg gctgtaagtc
    16321 gataattgaa gacagtgcca tctcaccatc tccagcttcg ccgacataag tgattggata
    16381 ataagaacca aggacagtta tttgttcccg caagttattg cgaatgaggt attcgtcttc
    16441 gatgataaaa actttataca tgtttgtttc ctccaattgt tttgggagat agagggatca
    16501 tcagtgtgac agttgttcct tctcggtatt tgctgtcaat cgtgataggt ttttgatcat
    16561 ttccgtaata aagaaggaga cgcttgttta cgttgtaaag tccgtagccg atactgaaat
    16621 ctgaatcgat ccctttggct aactctttac gtatttctgc cagtttttct gcagggatgc
    16681 caatcccgtt gtctataact ttgaattgga tcatctcatc aattttgcat gtggtaatac
    16741 gaataaatcc tttgtgacgg acgtacttcg ttccatggta tacagcattt tcaatcaatg
    16801 gctgcaagat catttttaaa atagaaacag gaaaaagtgt ttctggtata tcgatcttat
    16861 agtcaagcat atggccgtat cttattttta agatgactag ataatcattg atatggctaa
    16921 tttctttttc aagaggaatc cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg
    16981 aaagtgcatg ggtcatagtc agagcttctt caaagcagtc ttgttcgatc aaagaaataa
    17041 tggcatccaa actgttataa ataaaatggg gagtaatctg agcttgcaat acacgaactt
    17101 cactttgtgc taaatggtat tgtttcaaag cgttctcccg taaagactgg tttagatctt
    17161 ccgccatttt attcatgcta gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta
    17221 aacgaaagcc aaggttccct gaagaaagtt gttctgacat ctcgatcagc tgattgatcg
    17281 gttcttggac ctttctctct aaaaagcgac gattcttttt cgcatagaaa aaaatggtac
    17341 taagaactcc aagttccact attgttaata gaatgagcga gcggacaagt gtcgtatttt
    17401 ttttgcttgc taagtcgatc tccacacgaa caaaatcttg taagatatct gacagcagtt
    17461 gagtgacaga atctacctga ttgatgatct cttcattggt atccataaaa tcttttgcca
    17521 agatattatt gatcagctcg ttttgataat cttccagtgt atcgatcgta cgtagcgata
    17581 catctaacgt actaatttct ttaagtgtat ttgtattatt tttgatctct cgaatatcat
    17641 tcctaagttc ttctaggata ttttcctcat tgaactcatc tacggttact tggccagaaa
    17701 tcaagtccca catactttcc aaaacttttt gatcaacctt caaagcaatt gcatctgctt
    17761 tttggatatt tgcgatcgtc tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc
    17821 tcagtagcaa agggataaca gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct
    17881 gttttatgct taaatttttt gtcatgatac ttctccagtc aataatttct ggttgggatg
    17941 ttagctatat tctgctggga aaagacagtc tcggaaataa aaatatcttt aggtaatgtt
    18001 tttcctgttt gaatatttcc aaaataacga gtaacggtat tggctacata ccagccgaca
    18061 tcaggtttac actcgactac acaattgacg attcctgagc gtagcttctc gatcatatct
    18121 tcttgtccat caatagttac aatgatcaaa tcttctccag gtttgattga tgtttctttt
    18181 atcgcagcta aagcaccgtg agtcatttca tcactatggc tgtataatac atcgatttcg
    18241 gataaatcat ttgtttctaa atatcgtttc atctggtctt ttcctttcat ccgaataaaa
    18301 tcaccgacaa ttcggtgaac aatagtcatc ggaggtttat ggtcgatggt tccatctttt
    18361 tttagggttg gtttacgatt gattgattcc aaaaatcctt cggagcgtag cttggtcggt
    18421 gtggagttct ctgaaccagc aagttctagg attctgattt cctgtttctt tgaatttgtg
    18481 aagtgattgc tgacaaataa acccgcccga tttccttctg ctttgaaact ggggccaatg
    18541 tgggtgacga aaagctcggg atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg
    18601 atccctgctc gctttgcttc ttttaataca gagtcccacc cggattcttg caaaggagta
    18661 aaaatgatca tatccacttt ataggcaatg aacgtccgta tatcttggat ttgccgttcc
    18721 tgattcatgt agccatttcg gtagagaact tggtaatctt ctttttcaag cgcagtgata
    18781 attgattcag tatgcttttt ccgccagcta ctctccgttc ctgattgcga aaaaccaacg
    18841 actagtgggt caggtgcttc ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt
    18901 acaacaaggg ctaaaaaaaa taaaacacgt ttcactttaa atcactccat ctggatttgt
    18961 tagtactatc ttatcagata aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa
    19021 tccaagaaac ttaaaaaaat ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat
    19081 cccataaaaa aatcaatgtt aacgctttct ttattagcta tactcggtgt atcaattgaa
    19141 atgggggata aaaaatgaaa aacaaattta gaaagattgt tgggactatc gctattttga
    19201 ccgcaagcat tctattaagt gcgtgtggca attcaggatc tgcagatgat agtacggggt
    19261 atgtaggaat cgctatgccg accaaatcag cagaaagatg gatcgctgat gggaacaaca
    19321 tggtctcgga attagaaaaa ctgggttata aaacagatct tcaatatgga gaagataaag
    19381 tagaaaatca agtagcacaa atagaaaata tgattacaaa aggggtagat acacttgtga
    19441 ttgcttctat cgatggttcg gctttaactg atgtgttagc taaagcaaaa gaagcagata
    19501 tcaaggtgat cgcctatgat cgtttactga tgaattcaga aaatgtcgat tattacgcga
    19561 cttttgataa ttttggagta ggtgtatcgc aagcagctta tattgaagag cacttaggat
    19621 tgaaagaagg aaaaggacca ttcacgattg aattatttgg tggctcgcct gacgacaaca
    19681 acgcactaat caattacaac ggagtgatgt ctgttttaca accatatatg gacaacggtc
    19741 agttggtcgt gccatctgga cagacgagtt tcagtcaaat cgctacgctt cgctgggatg
    19801 gttctacggc gcaagcaagg atggataatc tgttaagcgc aaattatact gaccaaacat
    19861 tggatgctgt tttgtcgcca tatgatccaa taagtttagg aatcatttcg tcattaaaag
    19921 gcgtggggta tgggagtgaa agtaaacctt tacctgtgat tactggacaa gatgcgacag
    19981 ttgctggcgt taaatcgatc attgccgggg aacaaacaca aacgatcttt aaagacacac
    20041 gtatcttagc aaaaaatacg attgaaatga tcaaagcaat cagtgatgga gaagaggttc
    20101 cggtcaatga tactgaaact tatgataatg gggtgaaaac tgtccctacg tatttagcaa
    20161 atacagtatc tgtcgataaa gataattatc aagcagagct gatcgacacc gattattata
    20221 aagaatccga tctgaaaaat taagaggaac caaacaagga actaaagaga tctaagtcag
    20281 agagaggata agaattatgg cggattgtat tttaaaaatg gcagatatca ccaaaaaatt
    20341 ttctggaatc cgagcgttag attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc
    20401 attgtgcgga gaaaacggag cgggaaaatc aacattgatg aatgtcttga gcggcttgta
    20461 tccatatggc tcctatgacg gggaaatctt ttatgatgga aagctgtgtc aatttaaaga
    20521 tttgaaagat agtgaagaaa aaggcatcgt catcattcat caagaattag cactaagtcc
    20581 ttatctttca ataaaagaga atatttttct cggaaatgaa caagcagaaa gaggcgtgat
    20641 tgattgggaa ctgacagaaa agaaaacata tgaactgctg aataaagtcg gtttgtctat
    20701 cgacccgaat cttctagtat cccagattgg cgtagggcaa cagcaactag ttgaaatagc
    20761 taaagcatta tcaaaatccg tccgtctgtt gattttggat gagcctaccg ctgcattgaa
    20821 tgaagaagaa agcgcgaatt tgctgagatt gatccgagag ttgaaagagc aaggtgtaac
    20881 ctccatcatt atttcacata agctgaatga aatcgtggat atcgcagatc gaatcaccat
    20941 tttacgggat ggacagacga ttgagacact agaaaatgaa atgatcagcg aagagcatat
    21001 tattcgcgga atggtgggca gagatttagt tgatcgttat cctgaaagaa aaccggatat
    21061 tggagaagtt tattttaaag tagaagactg gacggtctat caccctcttg atcacgagcg
    21121 aatcataaac gaccacatca acttgcattt gaaaaaagga gagattgtag gaatagctgg
    21181 actgatggga gcgggcagaa ctgaattcgc tatgagtcta tttggacatt cttatggaag
    21241 caagatatcc ggacgtatat taaaggaagg ggcagagata tcagttaaga ccgttccttc
    21301 agccattgca aacggactcg cttatgtctc ggaggaccga aaatctttag gactgaatct
    21361 gctgatggat attcgggaaa acgtgacgct gagcagtttg gataaaataa gtcaaaaagg
    21421 agtattagat aaagaaaaag aagtaactga ggcagaaaat ttccggaaaa agatgcgtat
    21481 aaaaacaaac tccatccatc aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt
    21541 cgttttatcc aagtggctga tgacacaacc cgatattctt tttttggatg agccaacccg
    21601 tgggatagat gttggggcaa aatatgagat ctacacgatt atcgaagaaa tggcagcaaa
    21661 tggcaaagct gtctgtctta tctcttctga acttcctgaa atattaggga tgtgtgatcg
    21721 aatctatacg atgaacgaag gacgtattac aggagaagtc tcgcgagaag atgcagatca
    21781 ggaagtcttg atgaaattaa tgacgaaaga agaggtgggt taagttggaa caggcaaaaa
    21841 atcttacgga taaaaaagag aaaaaactaa atttgaaaag tagagcagct gatctttttg
    21901 gtcgctacag catggtgatc attttagcgg gactacttat tgcgtttcaa ttgatgacgg
    21961 atggtatttt ctggcgtcca ttgaatataa ctaaccttgt tctgcaaaat agccatatat
    22021 tagtactagc tgcagggatg ttattagttg tgctcttagg tcatgtggat ttgtcggtag
    22081 ggtcagtgat ggcgtttgtt ggtgcaattg cgggcatgat gatggtcaat cgccaaatga
    22141 acccgtggat gacagttatc gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag
    22201 gtttttgggt agcttatgca gggataccgg cgtttatcgt gactttagca ggattgctga
    22261 tgtttcgtgg actgacacaa gttgttctag gaggccaatc tctagcacct tttccagctg
    22321 ttttccaaaa aatgtctacg ggttatttac cagacttaag cgaaggaagc agccttcact
    22381 tcttgacatt gattttagga atgatcatca cgatcgtgtt agttgttggt caatggcgaa
    22441 caagaaatcg tcggaaaaat catttatttg aagtggaatc tccaacagct ttcttggtaa
    22501 aggcggtatt tacagcagtc gttgtactag gaataactta tatctttgcc tcttatcaag
    22561 ggtatccgat tattttgatc attcttggtt tgatcgttgc agcatatggg tttttgacaa
    22621 ataaaacagt agcaggtcga cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac
    22681 tatcagggat caaaacaaag cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg
    22741 cagctttagc tggattagtt ttagctgctc gattgaatgc ggcaacacct caagcaggta
    22801 catcgatgga actggatgca atggcggcag tatattttgg tggtgcttcg acttccggcg
    22861 gcattggaac aatcatgggt gcgattgtcg gtggcttggt catgggtgta ttgaacaatg
    22921 gcatgtcgat tttaggcgtc ggggtagact ggcagcaggc aatcaaagga ttgatcttgc
    22981 ttttagccgt tgtgctagac ttgtataata agaaaaaacg aacaacgtag gagaacgccg
    23041 gttattcaga aaaaagactg tgacaagaca tttgtcacag tctttttttc gctaaaagcg
    23101 aacattcatt gtttcgtttg tatagactaa aaataatata tgttttttta ccattcgtat
    23161 aaattctctt tttgctcttg atttttttgc ttaattgagt taaattacat agggcgctaa
    23221 aaaaagcctt gattaaatta ataatgttcg tgttttgctg gatgtgaaca tcaggaggat
    23281 aatgtaatga aagtatttga ttacgaagat attcaattaa ttcctaataa atgtatcgta
    23341 aatagccgtt ctgaatgtga tacaacggtc acattaggta aacatacatt caaaatgcca
    23401 gtagtgcctg ccaacatgca gacgattatt gatgaaacaa tcgcagaatt cttagcggaa
    23461 aatggttatt tctatatcat gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa
    23521 aaaatgaaaa agcgtggatt gattacctcg atcagtgttg gcgtcaagaa agaggaatat
    23581 tcctttatag agaagttggc agaagaatcg ctaaatcctg attatatcac tatcgacatc
    23641 gcgcatggac atgctaactc agttattgat atgattcagc atattaaaaa atatttgcct
    23701 gaaacttttg tgattgccgg taatgttggt acaccggaag ccgtccgtga attggaaaat
    23761 gcgggcgcag atgcaacaaa agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc
    23821 aagacaggct ttgggactgg aggctggcaa ctggcagctc ttagatggtg tgcgaaagca
    23881 gctcgcaagc cgatcatcgc tgacggtggg atcagaacac atggcgatat tgcaaaatct
    23941 gtccgttttg gtgcgacaat ggtcatgatt ggttcattgt ttgccggtca cgaggaatct
    24001 cctggcgaaa caaaagtcga aaatggaatc gtgtataaag aatactttgg ttctgcttca
    24061 gaattccaaa aaggcgaaaa acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa
    24121 ggatctttga aagatacact agtggaaatg caacaagacc ttcaatcatc tatttcttat
    24181 gctggaggaa gagatctgga agccattcga aaagttgact atgtcatcgt gaaaaattcg
    24241 atttttaatg gagacacaat ctaaaaaata aataaaatat taggatgttg tgaaaaagtt
    24301 gtcctgcatt aagtaataag aacagccaaa caaaaatagt tctttatatg ttttgtttgg
    24361 ctggaattat tatcgcagat acaagctttt gaacatcatt attcagaata agggactgtg
    24421 ataagatttt tgtcacagtc ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa
    24481 attcggtaaa atcaaaagaa aaaatagaca atagaaaaga gaagcttatg tgggaaaaag
    24541 tagagaaaaa acaaggaccg atttatcgtc aaattatgga acagattatg agagaaatcg
    24601 aaaacggaag gatcaatcct ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt
    24661 ttggtgtgaa tcgttccacc gttgtccatg cattggaaga gcttagggag cttggggtac
    24721 taacgagcaa acgtggcagc ggatggtttg ttaatcagac tgaatggggg aaattcgctg
    24781 ttccaagaat cgattggcgg caaatgatct ctcctcgtta tgagcagacg gactggtatg
    24841 agcaaaaaat aagagaagca aagcagatcc aaaaaacatc gtttcttgat ttatacgcta
    24901 gcgagatgcc agatgagctt ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa
    24961 tcttagcgga agaaaaagaa atggatcttt taggctatac tccgttacgt aacaaaatca
    25021 aacggtattt ggaaagtaaa tttgaattgg cattttcccc caaccagctt cttgtgactg
    25081 gtggcggtca gcaagcaatc tttttgattt tacaaacgat tctttcagct ggagagggga
    25141 tagctgtaga gtcaccttct tttttttacc gattacctct tttcaaagct tcaggaacgc
    25201 gtttatttgg gataccgatg gatgaagaag ggatcgactt agaaaaactg aaagaatcga
    25261 tccataaaaa taaattgaaa gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa
    25321 aagtgatgag tcaaaaacga cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc
    25381 caattattga agacgatgta tttacggatc tttcatttct aaaccaagaa gaagtccttc
    25441 ctatacgaac gattgatccg gataatgtgt tatacgtcgg ttcgttatct agggttttag
    25501 gaaaaacaac gaaaatcggt tgggtgatag gacctgagac ttttatctcg caactagctc
    25561 aagcgcaaga aatgatggag ttttcgatga atatccttac acaaatcaca gtggctaatg
    25621 tatttaatga agcaattgat ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa
    25681 gccgactgat aagcgcttgg ggagattcac aatcgttttt caagcttcat tcaattttag
    25741 gtggttacta tgcctggatt acttgggatg ggaaaaaact agaccccgaa ctagcagaaa
    25801 aagtagtaga tatgggtctt ggaattgcac caagtctact ttttgggcaa gatataaatg
    25861 gaatcagaat caattacagt aggctggatg aaaaccaaat accgttgttt tattcaaaga
    25921 tgaaaattct agaaaagtgg ttaacagaat aatttcacaa agtaaataga ttaaagaata
    25981 taacagcttg gatttaaatt tcaagctgtt tctttttttt cggaaaaaga atacgatata
    26041 aagaaactaa gaaaaaacca ttatttgtac gtccgttcac gatgtgtaaa taaaataaat
    26101 gtttgcaaca aaactgtata acaccaacgt ttttaaatag aaacagatgg ttgtaagttt
    26161 gattagaaaa tagaaaaaat gcagatgaaa tataaaacgc tttccttatt gtgattttat
    26221 tcactaatgc tgttaatatg cggattggca taatttcctt tcatcgtgaa aatttgtgct
    26281 tttagcttgt atttctcata aatccatgat aatttattaa tgaagacggt taaatagtga
    26341 tacagtttta ttcactgttt caatcgcaga atgaagagat tttcttatag atgagagagg
    26401 aatgtgtgac aaatggcaaa caaaaaagca ccgatcgact tccaagcact gctagatgaa
    26461 gtcaatgcag atttcccagt atatcaagca cttgatcaag atggaaacgt agttaacaaa
    26521 gatttagtac ctgatttatc cgatgatgaa ctcgttgaat taatgactca tatggtatgg
    26581 tctcgtgtat tagaccaacg ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt
    26641 gcaccaactg ctggacaaga ggcaagtcag ctggctagtg cttacgcatt tgataaagaa
    26701 gatgttcttc ttccaggtta tcgggatgta cctcaactag taaaacatgg tttaccttta
    26761 tctcaagctt ttttatggtc tcgaggacat gctgcaggta attcttatcc ggaagaattg
    26821 aaagctctac caccacaaat tatcatcggt gcacaatatg tacaagcagc cggtgttgca
    26881 ttaggtttga agaaacgaaa caagaaaaat gtcgtcttca cttatactgg tgatggcggt
    26941 tcatcacaag gtgattttta cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc
    27001 gtattttata tccaaaacaa cgggtatgcg atctctacac ctcgtgaaaa acaaactgca
    27061 gcaaaaacac ttgcacaaaa agctgtcgca gctgggattc ctggtattca agtcgatgga
    27121 atggatccgc ttgcagtata tactgtatct aaaatggcac gggaatgggc gatcagtgga
    27181 aatggaccag tcctaatcga aacactgact tatcgttatg gaccacatac actatctggt
    27241 gacgatccaa cccgctaccg tacaaaagac acagatgacg attggcagaa aaaagatcca
    27301 ttgatccgct tccgtaaata tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa
    27361 caagtcatcg aagcaactaa agaagaaatc aaagcagcta ttgctgaagc cgacaaagta
    27421 ccaaaacaaa aggtttcaga tttcttgaaa aacatgttcg aagtatctcc acaatcaatc
    27481 aaagaacaaa ttgcaattta tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa
    27541 tgatccaagc aatcacagat gccttagcac ttgaactaga aaatgacgag aatgtcgttg
    27601 tttttggtga agatgttggg aaaaacggag gagttttccg ggcaactgaa ggattgcagg
    27661 aaaagttcgg agaagaccga gtattcgata ctcctttagc tgaatctgga atcgcaggat
    27721 tgagtttcgg tcttgcgtta gaaggatttc gtccagttcc tgaaatccaa ttctttggtt
    27781 ttattttcga agcaatggac gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga
    27841 gcggcactag aaatctaccc atcacaattc gttcgccatt tggtggcggt gttcatacac
    27901 cagaattgca ttcggataat ttagaaggtt tgatcgctca gtctcctggt attcgagtag
    27961 tgattccttc caacccttat gatgcaaaag gactattgat ttcttctatt agaagtaacg
    28021 atccggttgt tttcctagaa cacatgaaac tatatcgctc attccgagaa gaagtgccgg
    28081 atgaagctta cgaagttcct ttggataaag cagcagttac tcgtgaggga acagatgtat
    28141 ctattatcac atacggagca atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa
    28201 aagaaaatat atctgttgaa atcattgatc ttcgtacagt tgcgccatta gatgtagaaa
    28261 cgatcattca atccgtggaa aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac
    28321 aagcgggcgt tgccgcgcaa gtagtttctg agatctcaga acgcgctatc ctttcattag
    28381 aagccccaat cggacgagtt tctgcacctg atacagtctt cccatttgga caagcagaaa
    28441 atgtctggtt gccaaatgca aaagatatcg aagacaaagt aaaagaaatc gcggaatttt
    28501 aagaaaagaa gggacgaaac aaagtcatgg cttatcaatt taaatt
//
LOCUS       DACEMO010000015        27479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEMO010000015 DACEMO010000000
VERSION     DACEMO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.015"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(128..931)
                     /locus_tag="IW728_000486"
     CDS             complement(128..931)
                     /locus_tag="IW728_000486"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1023867.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(951..1793)
                     /gene="kduI"
                     /locus_tag="IW728_000487"
     CDS             complement(951..1793)
                     /gene="kduI"
                     /locus_tag="IW728_000487"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ1023868.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(1809..2816)
                     /locus_tag="IW728_000488"
     CDS             complement(1809..2816)
                     /locus_tag="IW728_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ1023869.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(2823..3467)
                     /locus_tag="IW728_000489"
     CDS             complement(2823..3467)
                     /locus_tag="IW728_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1023870.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(3567..4325)
                     /locus_tag="IW728_000490"
     CDS             complement(3567..4325)
                     /locus_tag="IW728_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ1023871.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(4452..5603)
                     /gene="fucO"
                     /locus_tag="IW728_000491"
     CDS             complement(4452..5603)
                     /gene="fucO"
                     /locus_tag="IW728_000491"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ1023872.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(5685..5996)
                     /gene="rhaM"
                     /locus_tag="IW728_000492"
     CDS             complement(5685..5996)
                     /gene="rhaM"
                     /locus_tag="IW728_000492"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ1023873.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(5998..6843)
                     /gene="rhaD"
                     /locus_tag="IW728_000493"
     CDS             complement(5998..6843)
                     /gene="rhaD"
                     /locus_tag="IW728_000493"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ1023874.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(6857..8113)
                     /gene="rhaA"
                     /locus_tag="IW728_000494"
     CDS             complement(6857..8113)
                     /gene="rhaA"
                     /locus_tag="IW728_000494"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ1023875.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(8110..9570)
                     /gene="rhaB"
                     /locus_tag="IW728_000495"
     CDS             complement(8110..9570)
                     /gene="rhaB"
                     /locus_tag="IW728_000495"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ1023876.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            9723..10592
                     /locus_tag="IW728_000496"
     CDS             9723..10592
                     /locus_tag="IW728_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1023877.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(10718..11617)
                     /locus_tag="IW728_000497"
     CDS             complement(10718..11617)
                     /locus_tag="IW728_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1023878.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(11782..12606)
                     /locus_tag="IW728_000498"
     CDS             complement(11782..12606)
                     /locus_tag="IW728_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1023879.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(12617..13912)
                     /locus_tag="IW728_000499"
     CDS             complement(12617..13912)
                     /locus_tag="IW728_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ1023880.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(13928..14407)
                     /locus_tag="IW728_000500"
     CDS             complement(13928..14407)
                     /locus_tag="IW728_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1023881.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(14413..15441)
                     /locus_tag="IW728_000501"
     CDS             complement(14413..15441)
                     /locus_tag="IW728_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ1023882.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(15458..16564)
                     /locus_tag="IW728_000502"
     CDS             complement(15458..16564)
                     /locus_tag="IW728_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1023883.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(16793..19390)
                     /gene="adhE"
                     /locus_tag="IW728_000503"
                     /gene_synonym="adhC"
     CDS             complement(16793..19390)
                     /gene="adhE"
                     /locus_tag="IW728_000503"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1023884.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(19695..20075)
                     /gene="yajC"
                     /locus_tag="IW728_000504"
     CDS             complement(19695..20075)
                     /gene="yajC"
                     /locus_tag="IW728_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1023885.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(20172..21317)
                     /gene="tgt"
                     /locus_tag="IW728_000505"
     CDS             complement(20172..21317)
                     /gene="tgt"
                     /locus_tag="IW728_000505"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1023886.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            21535..22233
                     /locus_tag="IW728_000506"
     CDS             21535..22233
                     /locus_tag="IW728_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1023887.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(22401..25040)
                     /locus_tag="IW728_000507"
     CDS             complement(22401..25040)
                     /locus_tag="IW728_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1023888.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(25659..26690)
                     /gene="queA"
                     /locus_tag="IW728_000508"
     CDS             complement(25659..26690)
                     /gene="queA"
                     /locus_tag="IW728_000508"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1023889.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            26883..>27479
                     /locus_tag="IW728_000509"
     CDS             26883..>27479
                     /locus_tag="IW728_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1023890.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         7623 a         6117 c         4848 g         8891 t
ORIGIN      
        1 cttggagtta aatgcttctg tcccagcctc ttttttcgaa taaatgatgt ttaatcagct
       61 gtccttcggt attcgttcaa tccgctgatt tcacaacctc tccactaaac agtgtactaa
      121 agctctttta aggttgtttt ccgatatagg caagtatgcc tccgtccaca tacaagacat
      181 ggccgttaac gaaatcagat gcgctagaag ccaagaagac agatggtcca gccaaatcga
      241 ttggctcgcc ccagcgtgct gctggcgtac gtccaacgat aaattgatcg aatggatgac
      301 gttcaccgtt ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca atataacctg
      361 gtccaattcc attacattgg atattgtatt gaccgtattc agaagcgatg tttttcgtca
      421 gcatttttaa tccgcctttt gccgcagcat aagcactgac cgtttcgcgt cctaattcac
      481 tcatcatgga gcagatattg atgattttac cgccgccttt ttcgatcata tccggaataa
      541 ctgcttttga aacgatgaat ggggcattta aatccacgtc gattacttgg cggaagtctg
      601 ccgcatccat ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg actagaatat
      661 caactgaacc tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg
      721 tcacatcgca gacataacct tttgcatcga tgcctgcttc tttataatgc gctatacctt
      781 catcaactga ttcttgattc aagttattaa atacgatttt tgctcctgct gtagctaaag
      841 agcgtgcaat ctcaaatcca ataccataga cagcaccggt gactaatgct acttttccat
      901 ctaaacgaaa catatccata ctaaattcca tcattttctc tcccttatgc ttattttaat
      961 tgatccattt gcaccatatc catgtctgta taggtgatat tttctccgca cattgcccag
     1021 atgaatgagt aattgcttgt acctacacct gaatggatcg accagcttgg cgaaataatg
     1081 gcttgttcat tgtctacaac taaatgtttt gtttcatctg gtttgcccat catgtggaac
     1141 acacgtgtat cttcattggc ataatcaaag tagacataag cttccatacg acgttcatgt
     1201 gtatgacaag gcatcgtatt ccaagcgctt cctggttcta aaatcgtata acccatttgt
     1261 aattgacagc tttcgcatac atttggatgg atgtattggt agattttacg ttcattcagt
     1321 gtcaggcctt caccagtctc cattggtttt acttggtcga tactgatttt tacatttgga
     1381 tatttatgat gagctggaac agaactaata tagaatttag ctgggttttc agcatcttga
     1441 gaagaaaata caacatgtcg tgtttctttt ccgatatagt atccgtcttg ttttttcatt
     1501 tcttcttttt gcccatcgat ctcgatgaat cctggtccac cgatattgat cacacctagt
     1561 tcacgacgct ctaagaagta atcaacacct agttctttgt ctaatgtgat ttctaattct
     1621 ttttcagtag gtgtcactcc accaaagatc attcgatcgt tgtgcgtata agtcaaactg
     1681 attttccctg gttcaaatac cttttctact aaaaattctt ttctcaattg ttccgttgaa
     1741 taatgacgga tatcttctgg actgtgtgta taacgtgttt ccatttcttg cattttcttc
     1801 tactccttct atcgaatgat ttttccagag ccgcatgcta aaaattgtgc gacttcttct
     1861 tgactaaatt ggttgcagtc cccatggatc gtatgcttca aggcagaagc tgccgtagca
     1921 aaatcaatga tttcttgcgg agacatattg gataaaatcc catggagcac gccacctgca
     1981 aaagcatctc cgccacctac tcgatccacg attggggtga ttctatggac ttgggattcc
     2041 acatattcat ggttcatcca caatgtcccc ttcaattcat tttcacttgc tgaatgaacg
     2101 gtacgcttgg tcgaataaaa cacctgaata ttcgggaact tttcctgcat tttttgataa
     2161 taataaatta gaggttgttc ttctgcttct ggcgctttct cgatacctaa caaataaaga
     2221 gcatccattt ttccagctga gcagatatct actaaaggta aaagctgatg gattacttcg
     2281 ctggcttctt gctgtgtcca taattttcca cgatagttga tatcgaagct gatcaggcag
     2341 ccagcttttt ttgcagcttc tatcacctgt ttcgtcattt tttgccattt tttagacaaa
     2401 gctggggtga ttcctgaaac atgaaatata tctattcctt ggaataactc ctctagcgac
     2461 cattccaaat gatccatttc agcaaaactt gatccggcac gatcatagat cactgaggcg
     2521 gcgcgttcgc cactaccagt ttccatataa taagtaccta gtcttgatcc accagataat
     2581 acctgtttcg tatctactcc ataacattgc aagtgctttt tgactgcttt tcccaatgcg
     2641 ttatcaggta cttttgtagc aaaaatcgct tcatgtccgt aattagctaa agaaattgct
     2701 acgtttgctt cccctccgcc ataatgggca gcgaatcgtt cacttgaatc gatacgggtt
     2761 ccctgctctg tggacaatcg caacatgatt tctccgagtg tcactacttt tcccataagc
     2821 agctacctct tgatttcttt catttttgct gcatactgtt gcgcgacttc cgttactttg
     2881 tcaaaatctc ctgtagctgc gggtgctaaa aggttcccac ctactccgac tgttacgaca
     2941 ccagcatcga accactcttt catattgtcc agactcacac cgccagttgg catgatattg
     3001 acttgaggca tcggcgcctt gaaggcagaa atgatacttg gtccataagc gcttccagga
     3061 aatagtttga cgatatctac cccgcttttc agcgctgttt tgatctcggt aatagtcata
     3121 catcctggta agtaaggaat ttgatacaga ttgcacatct ctgccgtttc ttgatcaaag
     3181 gaagggctaa caatatactc tgcgccagct aagatagcca agcgagctgt gacagcgtct
     3241 aatactgtgc ctgcgccaat cactatttct ggttggtctt tgtagaatga caaaagttct
     3301 ttgattactt gatctgcttg agggactgta aaagtcaact caataccagt tagaccacct
     3361 ttgatgattg cgtggcaagc attcaaagct tcttctttgc tttttccgcg taccaccgca
     3421 atcactccgg cgtttttcaa tcgagataaa atatcaattt ttttcatctt ctccacctat
     3481 ttcctattta gaaattttat tttataactt ggaaatctat acccatatta tacggcagaa
     3541 aagaaaaatt gcaaacgctt ttttcattat ttttgaattc tttgctcgat ttcgcttttt
     3601 gtttgcttga gggcactgat tatttgttct tttgtctcat ctgttaaacg atatttcggc
     3661 atacttacac taaatgcacc aatgatttgt tcgtttttca cgattgaggc tcctatacaa
     3721 aaaatatcac gttcgatttc ttcatcgtca aatgcgatgt gggtttcttt taccgttttg
     3781 atttcctttt tcaaccgtaa tgcattggtg atcgtatatt ctgtgtaagg gatcaacgga
     3841 tgcttttcaa gataatcagt gtactgctct tcactgaatt ctgctaatac ggctttgccc
     3901 atcgcagaat tgtataatgg tcgggtgatt ccgatttttg aagacatgcg gatcgtttga
     3961 tgttttggat ctagtttgtt cacgtacaag atctcattgt tgtccaatac ccctaagtgg
     4021 atagtctcat cgatcttttg ctgcagctgt tctaaaaacg gtaaagtcaa ctctaccaaa
     4081 tcgatttgtt caatgttttt attcgcgtag cgaataagct ttgcacctaa acgatagttt
     4141 ttctctgaat ttttatttac gtagccgatc atcaacaaag tatctagtat tttcaagact
     4201 gtagaagagg tcattcccac accctttgcg atcgtctgca aagagacatc ttggttttcc
     4261 gctaaatagt ctagtatttt agaggctttg atcaatacag ttccataagg ctttttattt
     4321 tccatataat attctcctat ttctttataa gaaaaattat agcggaaata ccatcaattt
     4381 aaaagagata tccagatata aactaccatt tctctttaca ggcaatttaa tgttgaagat
     4441 ccgcttcgtt tttacatcaa actttgatat aaagcaatga tttcttctac tgtcgtttct
     4501 cttggatttc ctggtgtgca tacatctcgc aaagcatctt ctgcaattgc tggaatatct
     4561 gcttctttta cacctaattc agaaatggtt tctgggattc caaccgcttt gctcaaccga
     4621 tcgatctcac cacaagccgc ttctcttaca tcttctaaag acatttcagc tgctccttta
     4681 atccctagaa caagagcgat ctcacggtac ttttctccag tatattcttt gttatatttc
     4741 atgacagttg gcaaaagtgc tgcacacgct acgccatgag gaatgttgta ccatgcactt
     4801 aacgggtgag ccatcccgtg taccagtcct aaaccgacat ttgagaagcc cattcccgca
     4861 atatactgtc ctaatgccat tttttctcgc ccattttgat cgccatctac agaagcttcc
     4921 aaagaatggc cgatgatttc gattgctttt aaatgcagca tatcagtcat ttcccaagcg
     4981 ccttttgtga taaacccttc aatagcatgc gtcatagcat ccatacctgt tgatgcagcc
     5041 aattttttcg gcattcccat catcatatcg ctgtcgatga acgcgacgat tgggatatca
     5101 tgaggatcaa cacaaacaaa ttttcggtga tttacttcat ctgtgatgac gtagttgatc
     5161 gttacttctg ctgctgttcc agaagtcgtt ggtatagcta atataggtaa acatggattt
     5221 ttcgtatcag caacaccttc taaactaatg acgtctgaaa actctggatt cgtcaaaata
     5281 atcccaatgg cttttgccgt atcgattggc gaacctccac cgattgcgat caaacagtct
     5341 gcctctgcct ctttaacaaa agccactcca tcttttacat tttggatcgt tggattaggt
     5401 acgatcccgt cgtataaagc ataagcgatt gctgctttat ctagaagttc tgttactttt
     5461 gttgctatac cgtgctcgat caatcctttg tcagtaatga cagctgcttt gccgaatcct
     5521 cgctgctgaa attcagggac gatcgactca atcgctcctt ttccaaaata cgatgtttca
     5581 tttaaaatca ttcgattact cattttctat tcctcctaat ttgtattatt tttcacaata
     5641 aaaacgaaag aaccccaagc aaccggttgc ccagttgctt gttgctacag ttcaaagact
     5701 ggcatcaatt cccaactgac tggagaggca tctggatttg tctccatgat atcttccatg
     5761 tacttccacc atttttgatt gatagatgta tttgccattt tttcccataa cttttcatct
     5821 tcgatcacta agtaaccaaa taacaaactt gtactcggat caagatagat cgaatagtcg
     5881 atgaccccgt gttcacgaag tgctgtacgc atttctggcc aaagttcatc atgtcttttt
     5941 ttgtattctt cttgtttttc tgggtaaact ttcatgcaaa aagctttttt caccatgtca
     6001 gacacctgct ttcgtatgaa cttcctccaa ataacctgct tttggagtga caccaaacgc
     6061 atctgctaaa tgccacaggt tttcgtccgt gattgtttgc aagattggtc cttgcgcttt
     6121 gacgtacgta tagacttctg ctgccttttc tgctgtctcg atcaaaccaa agacttcatc
     6181 catgtctttt cctgacccgt agattccatg ttgtggccat aaaaccaatc gagtttcttt
     6241 cattttttca gctgttgctt ccccaatctc atttgttccc ggaaccatcc acggaatgat
     6301 ggaaattcct tctggaaaaa caactaagca ttccgtacac atttgccata gcgtacgact
     6361 aaaggaacgt tcgtctaatt catgcgtaaa actcatcgct aatagatgac tagcatggca
     6421 atgcataacg atccgattat ctgggtctac ttctaaacgg gcgatatgac tcatgaaatg
     6481 acttggcaac tcgcttgtag gaaggcattc gttttccagc ccccacaaaa gttccagtgt
     6541 tttgccatct tcagcaactc gaacgattcc aagattctcg gcaggttcgc tcgctacatt
     6601 tttaaaatat cttcctgaac cagttacaat aaaatagcat cctgctaaac ggctggcatc
     6661 aaaaatcatc gagatgcggc ggatcacttt ttcaggatcc aagaacggtg tgatctcttc
     6721 ttcttttagt aaataagaaa tattcccgcc atttctttca tcccatccca agcgataaag
     6781 attcgacgtc gtttcgatca tttctttcac ataaggcgct tgtaaaatat ctattttttt
     6841 caatatagcc acactcctat tttcttaatt ccaacacgtc tttttgatat tcctggactt
     6901 cttccagcca atccaaacca actggaactt gatttttgta acagaaataa ttccatacat
     6961 cggcatacgg gaaatctttc aattcttccg tataagccat tcttgaagta aagtcaaaag
     7021 ccagctccat ttctttcaat cgctcaactg gttccagcat agctttcagc agtgcttttt
     7081 gtgtatttct cgtaccgatg acccaagcag cgatacggtt gatcgttgca tcaaagaaat
     7141 ccagaccgat atttgttttt tccaacaatt cgttacggac taattcttta gcgatttcct
     7201 gcaattcatc atccatcaaa accacatggt ctgaatccca acgcactgga cgggaaacat
     7261 gcaacatgat tcctttgctg aataaagcta atgaagataa tttattggaa atcacttctg
     7321 ttggatgaaa atgtccagca tctagacaaa caagtttgtc acgagtcaac ccgtaaccca
     7381 tataaaattc atgggaaccg actgtataag cttcagcgcc taaaccaaat agtttgcttt
     7441 ctactgcatc ttgtacatac cgttcatcta actcttctgt aaaaatttca tcgagtgatt
     7501 ccattaaacg ttcccgtggg gtaaaacgat caatcggatt atctttcatc ccatcaggta
     7561 cccaaaagtt attgatactt gtttgcccag tttctttccc aaagtactcc gcgattttgc
     7621 ggcttctttt cccgtgttcg atccagtatt cacgaacttc tttatctgga tgagccaatg
     7681 taaagccgtc tttcatcatt ggatgcgaaa agaaagtcgg attgaagtca agaccgattt
     7741 cttctttttt tgcccactct acccattttt caaaatgttt cggctcaatt tcatttagat
     7801 cgacagattc ttctgtatcc aaatagatag catgaaggtt caatttgtgt ttgcccggta
     7861 tcaacgaata agccttctcc agatcttgtc taagctcttc aggtgttcgt gcggctcctg
     7921 gaaaattccc tgtcgccatg attccaccgg tcaattcgcc atccggtgtc aagaaacctt
     7981 tgacatcatc tccttgccag caatgcatag aaattttgat gtcctgtatc ttttctagta
     8041 cagcatctgt atctacaccg atagttgcat atttttcttt tgcttgttcg taatttcttt
     8101 caatcgtgtt catacgatta ctcctttgat tttattcacg taccattcca tcgtgttttg
     8161 cttattttca tctgggtaaa aagtttcaca tggaaacgac tgtttgatta catttcttgc
     8221 ttgttctata ttcgaaaact gacctgtact gagcatttgc atggcaacat tcccgattgc
     8281 tgtggcttca ctaggtcctg cttctacaat gattccggca taatctgctg tcaattgatt
     8341 cagtagacga ttattcgatc cgccccctac gatgtgcagc ttttccaatt tcgttccagt
     8401 taagtgttct actttgatca gctcttgtgc gtaacaaaga gcaagattat catacacaca
     8461 acgagcgata gctcccgatg agacagggac tggctgctct gtttcaaaac agtattgctg
     8521 aatctcttct atcatatttg ggggattcaa aaatcgaggg tcattgattt ccatcacttg
     8581 ctgcagcggt ttttcttttg cagcaagctc tgccaattcc gcataactgt actcgtactc
     8641 ttgcattcgg gcaacttcct ggatcaacca catccccatg atgtttttca aaaaacgcac
     8701 cgtgttatgg gcaccccatt cattcgtata attttcagaa aaagcattgc gagaaacatt
     8761 ggcgatcttt gtttcaatcc ctaaaagcga ccatgtccca ctgctgatat aaccccaacg
     8821 ttctccttga cctggcgttc ctaatacagc tgacgccgta tcatgacttg ctactgtgat
     8881 aaaggtcaca tctggcaaat catattttga aaattgttct ttttgcagat ctcctagtat
     8941 cgttcccggc tctgtcaatg gtggaaataa ttccttcgag acaccgattt tttcaagaag
     9001 ttctatttcc cattcttttg tccctgcatt cagcagctgc atagtagatg cattagtttt
     9061 ctccgtcacc atgttaccag taaagacata ccctaaataa tcaggtatca gtaacaattt
     9121 atgtgctttt gccaaatttt ctttttcttc agcaaacaat tggaacaagg tattgaaagg
     9181 ctggatctga atccctgtct gctcatacaa cttatctaga gaatagattt cagaaaactg
     9241 ttctactgct ccattggtac gcttgtcccg ataagcaatc ggatcattta tccgatcccc
     9301 ttgttcatca atcagacagt aatcgactgc ccatgtatcg atccccgcaa aaaggcgatc
     9361 aatccctgtc tgctttaatt tttcaaatcc aatcaaaatc tctttgataa gataatcgat
     9421 cttccagcga tcgtgaccgt ctttgttccg aaagccgttt ttgaaccggt gtacttcttc
     9481 caagatcaaa gaaccttttg catccctcgt acttttcatt agtcgtccgc ttgatgcacc
     9541 gatatcgatg gcaacaaatg ttggattcat ttctccacct cttttacttt attgttgatt
     9601 agcactttat taataaagcg ttatcattta ttgacaatgc tatcataata cagaatctta
     9661 tattttgctt cacactattt tggtatataa ttgcattatc ttgacaagtt gggagtgttt
     9721 acatggatta ctgggaaaaa gccaaccact catggtcgga agactcaaaa agagtgatct
     9781 tgacgcctac acaaagaagc aggaatttat ttttttatat ccaagaaatc ggttacttca
     9841 aagcaccaat cccttactat actgaacgag cacatttacc ttcttacttg ataaagttta
     9901 ctttgtctgg caaaggttcg cttacatata atgataatca gcaacagcta aaagctggag
     9961 acttattttt tattgactgc caagaatatc agcgttatca aacgatcagc gaagaaccat
    10021 gggaaatgga ttggatccat ttgaatggtc agaacgtcca atcattttat caggagttca
    10081 caaaagacgg ccataacacc ttccattcaa caggagatcc atcagaaaac aagatccacc
    10141 atttgatcac agaattgatc cacatgcaac aaggcacgaa tgcccgtacc gatttccagt
    10201 cctctgtagc tatccacgag ttgctgaatg aactgatttt gcaaaagtat cagctagatt
    10261 tcgaagaaga ggatattccc gcctacgttt acaaactgaa gcaatactta gacgaacatt
    10321 tcaaagaaac gattacactt gatcaattag aacaacaatt cttattgaat aaatatcagc
    10381 tgagcaaaga tttttccaaa tttatcggtt ctccaccaat cgattatttg atcagcaaaa
    10441 aactttctta cgctaaagac cttcttcggt atacagaatt tacggttcga atgatttccg
    10501 aagaaatagg gattgagaat tttgcttact ttagccgcct gttcaaagca aaaacaggac
    10561 tatcgccgag agactttcgt aaattcagct gattcatata atatgtacgt cattttttca
    10621 aaatattttg aaatatatag ataaaaataa aataaccgca gtttctgctt ctatgtaaac
    10681 aacagcagct actgcggtta ttttttagtg atcattctca ttgttcatct tcaaaatgtt
    10741 cataatgaac caactgtttc tgtgtatcac gtttcaaaaa ttctttaggc gttatgccga
    10801 actctctctt gaatgctttc agaaaatgcg gataagaaga aaaaccactt ttactatata
    10861 cagcactaga gctgcggtat tctcgcaaca agtgcttggc atagatcagc tttttcttca
    10921 ttacatactg atgaaacgta aatccagtgt actttttaaa ttctcgtgtg atatggtatt
    10981 tgctgacaaa aaactgcttt tccatttcct ctaaagaaag cggatgagat aaattttttg
    11041 agatatattg taacatttgt tccacacgct cattaggaac tacagcattc tgttcttgcg
    11101 cactctcttc tttttgtaca acttgattga gaaaaatcaa gtattctacc agagcttgtt
    11161 catattttat gtcttcaccg taaatttttt tatcaggagg atggtcgata aatgataatt
    11221 tttgcttcaa tacttcctgt tctatttgca atacacgact cctcttctct cccattggtt
    11281 gaaaacaagc cgatagatta gaccatggcg tggaacgact ttctagaaaa gaaggggtga
    11341 caaagatata gacacgctcg aatacatctg aagattggcg gacgatacga tgcaaatcat
    11401 tggaatggat gagcagcaca cttcctgctt tgatcgtaaa ttgtcgtccg ttcaaataaa
    11461 aagtcgcttc tccctttaat gtagcatgga tctcatagaa atcatgatga tgatagatcg
    11521 ttttattttg gctgacgaca tctttgacat gatagatttc atactgatca gaaaccatcg
    11581 tattgacttg ttcaccgctg attttctttt ggagcatgtg ccgcacctcc ttttgttcac
    11641 ttatgcacaa ttttattata ccacgttttt tgtaatcaaa aagagctgct tttatatact
    11701 cttataaaaa agaagagacg tggcatcttc tagccccgcc ccctccattc taaatattag
    11761 ttaaccaacg tcttctttac attattcaag aagacttctt ccataacgaa tcccatcatc
    11821 ttttgtttct tcaagaacca catacaggta cggacgattc ttcatgatga tttctttgat
    11881 ttttgtgtac tcgatcacac cgtctcccaa atttaccggc acgatttttt cctgttcgac
    11941 acgaaaatct ttcaaatgaa ccgcacatat cttttcacca aaacgttcaa aagcttcttc
    12001 taccaattgg acttgttctt gataatcact acttgtgatc aaattggtcg gatctaaaat
    12061 gattcctaga tagtccgaat ctactgcttg gatcagctgc tctacccttg ctaaagaata
    12121 caccggatga ttcaaacctg gttctatccc caccatcatt tggtattttt ctccagcttt
    12181 taccaagcga cggataacgg acacagcttc agcaaatgct tcatctgtga aattttcttc
    12241 cgtatattgg atttccggca aaacacatcc agtttccgaa gcaaccatcg acgcaccaaa
    12301 ggaagaagca tagcgaagat actgctcgaa ctttgtcagc aactcttgtc gtatcgttaa
    12361 atctggatga atcatattga tatagcaact taaaattcct acggtcactt cttgcttttc
    12421 taaaactcgt ttgacataat ttcccatccc agaattgatc tcatttgcgc cagaaggtat
    12481 ttcaggaaac gaaagaccta aggcgagctg cagtgtatga atcccttgtt cttttgtttt
    12541 ttctgcaaga tcttctatcg tttggacttc ggaaagatca tgtcctctaa ttgtaatgtt
    12601 caacataact attctcctaa ctcagcatat tgggtagcca catgacgatt tgcgggaagt
    12661 aagtaatcaa aaacaagacg actaagataa ccccataaaa aggaagaagc ggtttcagca
    12721 ttgcctctgc ttttacgcct cctacacttg caccgacata tagtcctgta cctactggcg
    12781 gcgtgatgga gcctacacac agattcatga ttgaaaacaa cccgtaatgt acaggatcaa
    12841 cccctacact tgtgacgatt ggtaaaaata ttggtgtaaa aatcaagata gcaggcgcca
    12901 catccataaa catacccacc agtaataaca caagattgac taataacaga ataataaatt
    12961 tattatccgt tacgcccata atcaacgaac taattgcctg aggaataccg gtaaacgcca
    13021 tggcgaaaga catcatggaa gaagtagcca ataagaacat gatcgtccct gacattgcta
    13081 ctgattgaaa caacatatta ggtatatctt ttagttttac cgttttatag taaaacatag
    13141 caatcaacag ggagtaaatg acagctatcg ctgaagcttc tactgctgta aatatccccg
    13201 tcaagatacc accgataatg atgacaatca atagaaggct aggaatcgcc tcttttatta
    13261 tttttccagc atgagccatc tgtccttttt cgacaacagg gtatttattc tttttagcaa
    13321 cgatgaatgc tactagcatg attcctagcg cccacaaagc gcctacgaga taacctccca
    13381 taaacaaagc agagatcgaa gcaccaccag caacaagtga atacatgata aaagcagtac
    13441 ttggaggaat gaccatacct gtcggagctg acgcgatatt gaccgctgtc gcaaacttcc
    13501 ggtcatatcc ttcttccact tgctgaggga tcaacactcc gccaatcgcg gtagaagcgg
    13561 caatggccga gctagagatg gaaccaaata aagcattccc taacacattc gtatgagcca
    13621 aagcccccgg cacgcgcccg acgaacaaca tcgcaaaatc cactagtttc ttagctatcc
    13681 ctccattatt catgataatg cccgagagaa cgaaaaacgg aatagcgatc agcgaaaaac
    13741 tattgatact tcccaccatt ttttgagctg aactaaaagc agaaacatca aacggtataa
    13801 ctaacaataa agtcatcatc gatgagaatg caatacttat agcaatcggc atccctaata
    13861 caagcaacac aacaaataca aaaaataaca ctaatccggc ttctattgcc attttattcc
    13921 tccattttta tattttttct tgaattgctg gcttgttgct taatgtatcg attagattga
    13981 tcaggctata gataatgatg agtacccccg atacaggtac tgacagataa acatatccca
    14041 taggaatact taaagaaggc gagatctgcc ccattgtcaa tgtgacagca ttccatccgc
    14101 catacaccat gatcatcgca gcaaatacca gaaataaact ttgaacaaca acttctaaaa
    14161 ttttactttt tttgccagtt aatttttcac ttaaaaccgt tacggctaaa tgtgcttttt
    14221 gtcccacaac atacgcggaa gacaacatgg caaaccagac aagaccgaat cgaacaagct
    14281 cttccgtgac cgtatttgga tttcctaaaa ccgttctgga aaatacttgc cataaaacaa
    14341 cagcgaccat cccaacaagc aaaacagctc ccaggatctc taataccttg tttaaaatca
    14401 ttttcatcac agctacgctc cttgctcttg gatcttttga taaaggtctt tgtaggtagg
    14461 atcgtttttg aatatatcat gaagtggggc aactgcctct cgaaacggtg tattatctac
    14521 atcattgaat tggactccat gttcttcttg tgctaaagct ttttcttctt caattgcttt
    14581 tgcaaaagct tcaatttcaa aaaccgttga ttctttcgct gcttcttcaa tggctttttc
    14641 ctgctcatca gttagctgtt ctttgatggc atcattcatg atgatgatat ctggcacacg
    14701 tgtatgctca tcataggaat agtatttagc cacaccacca tgtccagctg tatacaagac
    14761 aaattcattg ttttctgatc cgttgatcaa attggattga agagacgtgt acacttcatc
    14821 acttcccata ggtacgggcg aaccgcctaa aagctctacc attttgatag cggtttcgct
    14881 ttgcatcaca cggatcttct ttcccttcaa atcttccggc gtatggatcg gaccgtctac
    14941 catataaaag ctgcgctgac cagaatcata ataggtcaat ccggtaaatc ctaaattttt
    15001 ggtcgattgg tagattggct ccattatttc ttcattatcc atcaccttaa agaaatgttc
    15061 ttcatcattg aatagataag gaactgaaaa aatcgaataa tctttagaga aactttccaa
    15121 cgctgaggca cttacttttg tgaaatctac tgctcctgac tgtgtcaatt cgatcagttc
    15181 acgttctcca cccagttgtc catctgggaa atactggatc tgtacggaac catctgtttt
    15241 ttcttttact aaacgcccaa attcattcat tgcatcaatt actggctggc tgttgcttgc
    15301 ataagcaaag cgcaaggtaa ctatctcatt tacatctgca tctgcgacag ttgccacact
    15361 agaattattt ccgcagccgc ttagacctag cagacctagg ctaagcgaac ttgctgcaaa
    15421 cagcagtgat gtttttttca tatgggtttc ctttctttta acaataagag acgagcaatt
    15481 ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat tgctgtgatt ccgatattcc
    15541 gataaaaatc taaactgtcc cccattcctg tcccgattga aacgttttga acttctcctt
    15601 tttcatcgat ttgttcaagc aatccttgga tagcacgata agcgacttct ttatattctt
    15661 gtggtaaata acgtttatgg accgctttca aaataccgta agcaaatcct gcagttgcag
    15721 aagattctac gtaagaagaa ggatcatcca acaacgtatg ccataaaccg ctttcagact
    15781 gataagtttt taatgcacgc acttgtgctt caagtgtttc gatcaataat ttgcgcaatg
    15841 catcttcttt cgataattct aagatttcaa tgatctctgg aatagcaatc gtcaaccagc
    15901 agtttccacg agcccaaaat gcttcggcat agttatgatt tccttcaaac gtccagccgt
    15961 gataccacaa tcctgttcgt ttatccatca agtatttgat atggatcata aactggtaac
    16021 gagcttcttc taaatattct gggcgattca acagtttccc gatttttgcc aaaggaagca
    16081 ctgtcatcat caatgtatcg tcccacaact gattcttatt ttccggtcca tatgtggcat
    16141 gctgtaaccc gtcttcattt gtccgaggca tgtcattcat gacccattct gcccattgtt
    16201 ctaaataagg caaatagcgt gaatctttcg tgtcttcata caagtaagcc atagtcaata
    16261 gcggtgccat tgtattgacg tttttgggcg gagcgccttc ttccatacga tcctcaaacc
    16321 attcattgac cacttgataa gcgcgaggat tttttgtcat ctggtaattt ttataaatac
    16381 catatagacc gacaccttgt ggccaattcc acactgtcca gcttttgtca tctactttca
    16441 gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt gatcgttgtc aggttttcaa
    16501 cgagcaggtc aattttttct ttcactaatt ctttaggtac aaaaatctct tctttaataa
    16561 tcatctttac gccacctttt taaacgcttt cattttatat ccttaatata gcagtaatac
    16621 cgtccactat aaatatcgct ttttgaacaa aaactgtcta aaattgacct aaatgttaaa
    16681 taacaaaagt aactttcagt tacttggaaa ttaaaaaaag aacttggaga aacaacgttt
    16741 tccaagttct ttttcatcta ttttcttatc ttttttctac gctttctttt gcttatccgt
    16801 tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa tggttctttc ggattagctg
    16861 tcgtacattg gtcttcgtaa gctaattcag ccatacggtc tacggttgta tctagtgttt
    16921 cttgtgtgac tccttgagct tttagactca tgttgatgcc gacagatttt cctaattcat
    16981 aaacagccgt tgctaaagct tcgactaatt cctctgttgt ttcgcctttc agacctaaga
    17041 attttgcgat atctgcgtaa tctgtatctg cacggaaata gtcatattta gggaacatcg
    17101 catgtttttg tggatctttt gcattatagc ggataatatg cggtaaaaga atcgcatttg
    17161 tccgtccatg agggatcccg tattccccgc cgattttatg tgcgactgag tgacaaatac
    17221 ctaggaatgc gttagcaaac gccataccag ccattgtaga agcgttgtgc attttttcac
    17281 gtgattccat atcaggtgtt tttactgatt tttctaaata gtcaaagact aacttgattg
    17341 cttgtaggct caatccacgt gtataatctg aagccatgac tgaaacatag gattcgattg
    17401 catgtgttaa cacatccatc ccggtatctg ctgtgacaga tgctggaact gacatgacga
    17461 attgaggatc gacgattgcg acatctggcg tcaatgcata atctgctaac ggatatttca
    17521 catgtgtttc actgtcagtg atgaccgcaa atggcgttac ttcagaacca gtccctgatg
    17581 ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc gatcttatat gtccgtttac
    17641 ggatatccaa gaatttttgt tttgcaccaa agaaagatgt gtctgggtgt tcatagaaca
    17701 tccacattcc cttagctgcg tccattgcag aaccgcctcc aagagcgatt actgtatctg
    17761 gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt atttgtcgat gggttcggtt
    17821 caacatctga gaagacttcg atcttcacat catttttacg tcgtgccaat acttcacgaa
    17881 ctgtatcgca gtaaccgaat tggaccatac cagggtcaca aacgatcatg acacgttcaa
    17941 cgttttccat tttctctaag tacaatagag agttcttttc aaagaagatt tttggcggta
    18001 atttgaacca ttgcatgtta tttctccgtt tcgctacagt tttgacattg attaagttta
    18061 cagcagagac gttacgagaa acagagttct taccataaga accgcatccc aaagtcaatg
    18121 acggaatcat ttcgttatag atatcaccga tgccgccttc tgcagatggt gtattcacta
    18181 aaatacgaca agctttcatt cgaagaccga atttgacatg caactcttcg ttttctgaat
    18241 ggataactgc tgtatgtcct aatcctccaa gattcaacat accttcacac aaatcaaacg
    18301 catggtccgt gttgttagct ttgatcatgg caagaactgg agaaagtttt tctcttgata
    18361 gcggataatc tgggcctacg ccttccaatt cagcaatcag cattttcgtt ccttttggta
    18421 cactgattcc tgccaatttt gcgatttcca tcgcagaatg tccaacgatt gatggattga
    18481 ccgcatattt tccttcgttc atcactgcat cttctaattt ttgcaattca tctggtttta
    18541 cgatataaac ttggtgtgct tggaattctg cttttactgc tgcatagatt tctttatcca
    18601 cgatcacagc ttgttcagaa gcacaaatca ttccattatc aaatgttttc gaaacgatca
    18661 aatcgttcac tgcacgttta atttttgctg ttttttcaat ataagctggt acgttacctg
    18721 gacctacacc taaagctggt tttcctgtag agtaagcaga cttgaccatt ccagctccac
    18781 ctgtcgctaa gacgatcgcg atacctggat ggttcattag catagaagtc gcttcgatcg
    18841 aaggatgttc gatccattgg atacaatttt caggagctcc ggcagcgata gcagcatcac
    18901 gtaccactcg tgctgcttct gcagaagatt tttgggcact tggatggaaa gcaaaaacga
    18961 ttggatttct tgttttcaac gcgatcattg atttgaaaat cgttgtggaa gtcggattcg
    19021 ttgttggagt tactccgcaa accacaccga ctggttcagc aatttcaatc aacccttttt
    19081 ggacatcttc attgatcaca ccaactgttt tatcatgttt gatattatgc caaatatatt
    19141 cggacgcata catatttttg attgctttat cttcgtaaac accgcggcct gtttcctcta
    19201 cagccatctt cgctaatggc atatgttgat ccaaagctgc cattgccatt tcatgcacga
    19261 tatggtctac tttttcttga tcaaagcttt ccatttcttt caatgctgca tttgcttttt
    19321 ctgccaaatc atcaatcatt gcttgtacgt caattgtctt cgttttttct ttttctatta
    19381 ctttagccat cattatcctc ctaatgggtc atggttattt gttaaataat tcacatgtat
    19441 atcatacaat atttattttt ctatgtaaac acttttttgt gatttttttc acagttattt
    19501 ttaaatataa aattgcttta tttacaggtg tttgtataga aaaatatata aagttattta
    19561 ttattattta tatgacacac ccgattattc aatattgagc aatatatccc atgctcttat
    19621 ctctttcttt tttgaataaa aaatgaggta ggacatcttt ctgccctacc tcgtctttaa
    19681 ataagctttg atacttattt ttcttccttc tcttctgtct cttccagcgt ttctgttgtt
    19741 tcagttttta cttcaactgt ctcagctggt tcttctgttg tttcaacagt agtcgttgca
    19801 tcatttgtta cgacagtccc tggtttgacc gttttgattg ctgcacgatc gtactcaagg
    19861 aagatccctt cgcagtctaa aacaactgtc cgtttgtcat tgtcgatttc tgaaacaact
    19921 ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt tcatgctatc taacaagttt
    19981 tgacgttctt gctgttgttt cttttgcgaa cgtgacataa aaaaccacat tgctaatagc
    20041 gcgataaaca tcacaagcat tggtaatgtt cccatttttt tcacctcact aattctgtat
    20101 ctacaaaaaa cactttagca gaaaaatgct gataacacta gttttttact taatttttaa
    20161 aagattttcc tttagaagct ttttgcgttt tctttattga acccgtattc ttcaaaaaag
    20221 gcttctctaa attcaagcaa attatcatcc atgattgctt gacgtacttg tttcattaga
    20281 ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa tcccaaatgt ttcatcacat
    20341 ttgatcaaat ggcggatata ggcacgtgta tagtttttac atgtataaca atcacatttc
    20401 tcatcaattg gacggaaatc atgggcatat ttcgcatttt tcactactag acggccttga
    20461 gaagtcatac atgtgccatt tcgagcgatt cgagtaggca agacacagtc aaacatgtct
    20521 actccacgga taacaccatc gatcaatgaa tcagcagcac cgacacccat caagtaacgt
    20581 ggtttgtttt cggggatcaa cggtgtagtg aattctaata cacgattcat ttctgattta
    20641 ggttccccga cagataaccc gccgattgag taaccaggga aatccatccc gactagatct
    20701 ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt ggatgattcc aaataatcct
    20761 tgacggtctg gattagcatg agctttcaat cctcgttctg cccatcgaga cgtacgttct
    20821 actgattttt tcacgtagtc gtaactttca tcgaatggtg gacattcgtc aaaactcatc
    20881 atgatatcag agcccaattt attttggatg ttgattgctt tttctggtga taaaaacata
    20941 cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt cttcgatatt ccgcatatca
    21001 cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa tcggttgatc ccagttcata
    21061 aatttatgca gtccgccagc ttcttctact aagtcttcac cagggcgtag ccataaatga
    21121 tatgtattac ttaaaataac gccagcaccc atttctttca actcttctgg ggacattgtt
    21181 tttactgtag ctaatgttcc tactggcatg aacattggtg tggggaaagt tccgtgtggc
    21241 gtgatcaatt cccctaaacg cgcgccagta tgtttttctt tcttgattaa acgatagcga
    21301 atggcaggtt gcgtcattca tttccctact ttctatataa aatatcttga caactcccac
    21361 catcataatc gtttcaagtg ctttttgcaa gttccctcgg cttccttcta ctttatttaa
    21421 tattcttttt tttatccttt ttttatgaat aacaaaaaaa agaaaaacat tatgacagaa
    21481 atattttttg atacatggta tattggtgtt attaaagaaa aggagggaaa aatcatgcaa
    21541 tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa aaggacgttg gggacaagct
    21601 gttttactaa atctaatccc tacattgatt ggtattgcta ctattcttct tattatgatt
    21661 cctattatct tgtttgccac caccatatat gatggatcag cgagcgtctc agaagcgaca
    21721 acttcaggag gcggtagttc aggaggcggt ggcggaatta tttccagcat cattagcgct
    21781 ttgttcatga gcggtatttc ttggacttat cttgatattt tacgtggaca aaaagatcag
    21841 atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt tgtttatcgg cggagtgatc
    21901 agcttagctg tgctgatctc gatctttaca acattatggg gtttgctatt agttattcct
    21961 ggaatcatta aaagctattc ttattctcaa agctatttta tttactacga tatcgtccaa
    22021 gaaacaggac aaacacctaa agtactggat acgatcactg caagtcgaaa attgatggat
    22081 ggttataaag gacaactttt ctggttagat ctttcattta tcggctggca tatcttagca
    22141 ttagcaactg ctggtattgg ttatctatgg ctcagcccat acatttctgc aacaaaagca
    22201 gcattttatg aacaattgcc aaaagatatt taaaagagag tgtgacttgt gaccgagggg
    22261 gtggcaggag aacaccgttt atcgagaggc agtaaaagaa acattcagcc cattagcaaa
    22321 aaggaggtgt gacaacattc atgtcacacc tcctttttgt atagttccag taaaaagaaa
    22381 atcaacttag aaacagttaa tcattcctct tcttctgctt ccggtaatcg atggaacttg
    22441 atcagttcat tcaccgcgat tggtacgata gataaaatcc ctaccaagaa ccagtgattc
    22501 aaactgatgt gtactaggcc gaatagttct tgcgtaaatg gcagcaaagc aacagccgtt
    22561 gtaatagcta atgccagtaa cgccatttct accaaagctt tattggtcgc taaattgact
    22621 ttgaagatcg attggctgct gcgtacattg aacacatgca gaatcgatga ataagctaag
    22681 atcaagaatg caacagtttg accgacatgc tgacttgcgt ctacccatgg gctgatagta
    22741 tcgacataac tacctaaata ataaccaaac aaggttacga tggtgaagac ccctgcattt
    22801 atggcaattt tttgcaataa acctcgagca aagatccctt catttttcgg aatgggtggc
    22861 tgatccatga tgccgtgttc cgctggctct cttcctaaag caaatcctgg aagtccatca
    22921 gcaactacat tgacaaacag tagttgcaca gctgtaaatg gtgctcccca tcctaacagc
    22981 atcgcaatca aaacaataaa gatctccgaa atattacagc ttagcaaaaa gttgatagct
    23041 ttacgaatat tctgataaac cgaacgtcct tgactgacag cgtctacgat cgtcgcaaag
    23101 ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca catctgttcc tgtgatcccc
    23161 atggcacacc caacgttact tgctttcaac gctggtgcat cattcacacc atccccagtc
    23221 atcgctacga ctgcacccgt acgttgccaa gatttgacga tccggatctt gtcttctggc
    23281 gttacccgag catagacaga gagagctttg acacgcgcat ctaattcttc gtctgataat
    23341 tgatgcagtt ctgatcctga aagtgcttct gacggatcat tcaagatacc taattctttc
    23401 gctatggcac tggcagtcac cacatgatct ccagtgatca tgactgtttt gatgccagcc
    23461 tttttcgctc gcttgattgc tcctttactt tctggacgtg gtggatcgat cattccgatc
    23521 agaccaatca aacgtaagtc tttttccagt gcttcagaag aaatatcttg cggttcttcg
    23581 tcataagtag cataaccaac agcaatcact ctcagtgccc gtttaccaaa gcgatcatta
    23641 acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg gagcaagaac atcgaatgct
    23701 cctttggtga ttgagatgta tttttcgccc atttggtgaa ctgttgtcat catttttcgt
    23761 tcagaatcaa aaggaagctc gtttactcgt ggatattttt cttctaattc tgcctttgta
    23821 tgataattct cttcaacagc ccttacgatg gctacttctg ttggattacc tttgataaca
    23881 aggtcgtcat cttcttgctc gaccgtcaca tctgtacata gtgcagccat tttcaatatt
    23941 tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta cttcatcttc tttgctccat
    24001 acccggcgga cgcgcatttc attttgtgtc agcgttcctg ttttgtctga gcagataaca
    24061 ttagcggttc ctagcgtttc aactgctggc aactgtcgga tgatcgcatg tttcctcgcc
    24121 atcttctgca caccgtatgc aagtgtcaac gtaacaataa cagtcaaggt ttccggtaca
    24181 gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa ggagtggttc tccttgcagt
    24241 tcaccaatca caaatactaa agccgcagcc gctaaagcga tcatactgat tcgtttgcct
    24301 agctgattca atcggacctg taaaggtgtt ttttgcatag tattttcgtt caaaagatcc
    24361 gcgatcttac ccatttctgt agccatgcca atggcagtga cgatcgcttt cccacgacct
    24421 gcagcaaccg tacatccttt aaataccata ttaagttggt cacctaatga ctcatcttct
    24481 tcagcaataa atgctgcatc tttttcaact gcttcacttt ctccagtcaa cgccgattct
    24541 tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg cagaaatagc atcaccggac
    24601 tctaaaacaa gaatatcacc cactaccagt tgctctgact caacagtggt cactttgccg
    24661 tctcgtatga cagttgccgt ttgtttattc atatcttgca aggattccag tgctttttct
    24721 gcgtttcctt cttggacaat cgccaatacc gagttgatca caacgatagc gatgatcagc
    24781 agtccttcaa ataaatcccc atgttctgta gcaaatgcgg tataaaagga aatcaccgct
    24841 gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc gaaagaattt tttgatcatc
    24901 gattctttcg gcgcctcatc gaatttattc aatccctgct tttttcgccg ttcatccact
    24961 tcttgtgaag acaacccagt atctggatct gcttctgtta ccttctttac ttcttctatt
    25021 gtctgcttgt attcttccat cctattcctc ctttaaaatc ttcgtacaaa aaaaggtact
    25081 tattttacta taacatcctt tttttattta cttcaaataa agggcttatc gattatttgt
    25141 taagaaagcg taaaatttaa agtgcttata tttgcaaacg ctaccaaagg atgctattat
    25201 atagatgtaa ggaaggagga taccatcaaa tagacagaag aatttctcga tagaacagtg
    25261 taagcaaaca aagttgttag aagtacaggt ttcacccaga catgcgtgta gagtacaaac
    25321 ctataaatag tgaatgcgta tggatgtttg gatcacgaag tatccgaaac aagaagtaat
    25381 gcgatacgta acaattgaat aacgtaaacg tctgaaaatt gaataggaac aagagatgtt
    25441 tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta acatgaagaa attcaacacc
    25501 gtgctttgca tgtatgatgg aaagttacaa tcatttctgt ttatttgaat ccttccgagg
    25561 tacaagaaag ccgagcttat cgagagctcg gctttttctt tgtcataaaa tctttgtaaa
    25621 gcttttttgt tattcaaaac tagcggcttt tcataacctt atttcacgaa catcgcatct
    25681 ccaaaactaa agaaacgata gcgctcttca atcgcatgct ggtaagcaga taaagtcaat
    25741 tctcttcctg caaaggcact aactaacatg actaaagtcg attttggcaa atggaaatta
    25801 gttgagaaag cttctaccac tttgaattca tagccaggcg taatgaaaat atcagtccat
    25861 ccgctgtctg ctttgatttc tccattaaat ttcgttccaa tcgtttctaa tgttcgaatt
    25921 gaagttgttc cgacagccac gattttcccg ccatttttac gtacttggtt caatcgctca
    25981 gccgcttctt ccgtcaaacg gtagaattca ctatgcatat ggtgttcttc aattttatct
    26041 acgctgacag gtcggaatgt acctaaccct acatgtagtg tcagatagac taattcaaca
    26101 ccctttgctt cgatctcatc aagtaattct ttcgtaaaat gcaatccagc tgttggcgct
    26161 gcagcagaac cattttcttt cgcatagaca gtttgatagc gatctgggtc ttccagacgt
    26221 tctttgatat atggaggcaa tggcatttct cccaatgatt caagaatctc tagaaaaatt
    26281 ccttcatact caaagtcaat gatccgtccg ccgtgttcca attcttcttt cactactgct
    26341 tgcaatctgc catcaccaaa agagattctt gtcccaactt tggcacgttt cgcaggtttg
    26401 atcaatgtct cccacgtatc tccttgtgta ttcgttagaa gaaggacttc taaatgcgca
    26461 ccagtatctg gtttttctcc atataaacga gcaggcagaa ctcgtgtatt attcataact
    26521 agcgcatcac caggctgtaa ctcttcaatg atttcatgga aatgtttatc ttccatctct
    26581 ccagttttcc gatccaaaac aagtaaacga gaatgatcgc ggtcttttaa tggtgtttgt
    26641 gcgattaatt cttccggcaa gtcaaaatca aaatcttctg ttgttaacat atcgtcactc
    26701 actttctatc atgtgcttca tcattctatc atgtttttcg ttctgttggc aaaatcagca
    26761 ctttttcact tctatttttt gttttcctaa ataaaaaaac taccctattt gtattttttc
    26821 attgttttag gcatactagt aatagatagt tcttcattga aaatagaaaa agaggtggca
    26881 ccatgattga atttcaaaat gtgtcaaaaa tttataaagg tggaaaagtc gctgtagagg
    26941 acgtcaatct ttcatttgaa aagggtgagt tcatttgttt tatcggtacg agcggaagcg
    27001 gaaaaacgac gacgatgcgg atgatcaacc gaatgactga tccaacacaa gggaaaattt
    27061 tgattgatgg aaaatccatc caagaaattg atccagtgga attgcgacgt cagatcggct
    27121 atgttatcca aagtattgga ttgatgcctc acatgacgat ccgtgaaaat atcactcttg
    27181 tccaaaaatt gttgaaagtc agtcaagaag aacggaataa aacagctgaa aaaatgattg
    27241 atcttgtgga attgccccgt gagatgttag atcgctaccc acatgaatta tctggtggtc
    27301 agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa tcaagatatc atcttgatgg
    27361 acgagccatt cggtgcgctt gatccaatca ctcgtgattc cttgcaagac cttgtcaaag
    27421 atttacaaga gcgtttggga aaaacgatcg tatttgtaac ccatgacatg gatgaagcg
//
LOCUS       DACEMO010000016        25516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEMO010000016 DACEMO010000000
VERSION     DACEMO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.016"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(136..843)
                     /gene="deoD"
                     /locus_tag="IW728_000510"
     CDS             complement(136..843)
                     /gene="deoD"
                     /locus_tag="IW728_000510"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1023891.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(860..1678)
                     /locus_tag="IW728_000511"
     CDS             complement(860..1678)
                     /locus_tag="IW728_000511"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1023892.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IW728_000512"
     CDS             complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IW728_000512"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1023893.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(2967..3923)
                     /locus_tag="IW728_000513"
     CDS             complement(2967..3923)
                     /locus_tag="IW728_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1023894.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3923..5056)
                     /locus_tag="IW728_000514"
     CDS             complement(3923..5056)
                     /locus_tag="IW728_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1023895.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5049..6611)
                     /locus_tag="IW728_000515"
     CDS             complement(5049..6611)
                     /locus_tag="IW728_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1023896.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6823..7905)
                     /locus_tag="IW728_000516"
     CDS             complement(6823..7905)
                     /locus_tag="IW728_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1023897.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(7971..8369)
                     /locus_tag="IW728_000517"
     CDS             complement(7971..8369)
                     /locus_tag="IW728_000517"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1023898.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IW728_000518"
     CDS             complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IW728_000518"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1023899.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9098..10399)
                     /locus_tag="IW728_000519"
     CDS             complement(9098..10399)
                     /locus_tag="IW728_000519"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1023900.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10540..11556)
                     /locus_tag="IW728_000520"
     CDS             complement(10540..11556)
                     /locus_tag="IW728_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1023901.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11864..12469)
                     /locus_tag="IW728_000521"
     CDS             complement(11864..12469)
                     /locus_tag="IW728_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1023902.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12665..13588
                     /locus_tag="IW728_000522"
     CDS             12665..13588
                     /locus_tag="IW728_000522"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1023903.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13647..15212
                     /gene="guaA"
                     /locus_tag="IW728_000523"
     CDS             13647..15212
                     /gene="guaA"
                     /locus_tag="IW728_000523"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1023904.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15703..17334)
                     /locus_tag="IW728_000524"
     CDS             complement(15703..17334)
                     /locus_tag="IW728_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1023905.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17348..19021)
                     /locus_tag="IW728_000525"
     CDS             complement(17348..19021)
                     /locus_tag="IW728_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1023906.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19034..19351)
                     /locus_tag="IW728_000526"
     CDS             complement(19034..19351)
                     /locus_tag="IW728_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1023907.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IW728_000527"
     CDS             complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IW728_000527"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1023908.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21013..21798
                     /locus_tag="IW728_000528"
     CDS             21013..21798
                     /locus_tag="IW728_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1023909.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21801..22493
                     /locus_tag="IW728_000529"
     CDS             21801..22493
                     /locus_tag="IW728_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1023910.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22767..23138)
                     /locus_tag="IW728_000530"
     CDS             complement(22767..23138)
                     /locus_tag="IW728_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1023911.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(23538..23768)
                     /locus_tag="IW728_000531"
     CDS             complement(23538..23768)
                     /locus_tag="IW728_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023912.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(23862..24200)
                     /locus_tag="IW728_000532"
     CDS             complement(23862..24200)
                     /locus_tag="IW728_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023913.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(25128..25244)
                     /locus_tag="IW728_000533"
     CDS             complement(25128..25244)
                     /locus_tag="IW728_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1023914.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         7436 a         5603 c         4499 g         7978 t
ORIGIN      
        1 ttcacaacct cttggtaaac ggtgttcaaa agcttacatt tgcaataata agtccagcca
       61 agtcaaaaac atgaggagct atttttgctt ggatgttctt attacttaat gcagggcagc
      121 tttttcattt ccttcttatt ctgctatcgc tgtttccaat cctacttcga tcatttcatt
      181 gaacgtagat tgacgttctg cagctgttgt ttcttcaccg gtgatcaaat ggtcgctaac
      241 ggtcatcaaa cttaatgtct gcacatggta tttcgcgcct aagtaataaa gagccgctgc
      301 ttccatttcc actcctaaaa cgccaagttc agctaattgg aacgtctctg tcaaatcatc
      361 tttgtagaaa gaatcttccg aaagaacatt tcctacatga accgtatagc ccttttcttt
      421 agctatctca tatgctttca gtaatagatt gaaatcactg attggaggaa aatgaaaggc
      481 ttggaaattt ttttcgatca tcgaagaact ggtcgctgca ccttgtgcta gtactaaatc
      541 acgaacatgg acgtcttttg acaatgcacc gcatgtacct actcgaatca atttctttac
      601 accatacgac tggatcaatt catgggcata gatggtggct gatggcattc ccattcctgt
      661 tccttgaaca gaaatacgtt ctcctttata tttgccggta aagcctaaca taccgcgtac
      721 ctgattgtaa caaactggat cttccaaaaa agtctccgca atatatttag ctcgcaaagg
      781 atctcccgga agtaaaatct tatcggcgat ctcgccttct ttcgcttcaa tatgcacact
      841 cattttattg ttcctcctat tacaataatg tcaatgtttc tttaaccaat gctttaaaag
      901 attgtttgac tctctgagtt gtttctacaa cttcttcatg gctcagattc gcttgcatac
      961 ctgctgctaa gttggtaata caagagattc ctaacacttt taatccgctg tgagctgcta
     1021 cgatgacttc tggaacagtc gacatcccta ctgcatcggc tcctattgtc cgagacattc
     1081 ggatctccgc aggcgtttca taagttggtc cagaatagcc catatatacg ccttctttaa
     1141 gatccaaacc tagtttttct gctgctgctt ttgctgttgc ttgatagtca ggcgtataag
     1201 cttgactcat atctggaaat ctagggccca ttgcatcttc attcggaccg atcaaaggat
     1261 tttcaccagt aaaattgata tgatcggtaa tcagcatcaa atctcctggt acaaatgatt
     1321 cgttgacacc accacatgca ttagtcacaa tgactgaatg ggcccctagt ccggccatca
     1381 cacgtactgg ataggtcact gtttgcatgg aatgaccttc atagtagtgg aatctccctt
     1441 gcatggctaa cactttcttg cctgccagag acccataaac tagctttcct gcatgcccaa
     1501 ctactgtcga aacaggaaag aacgggatct catcataaga aatcattaca gcatcttcga
     1561 tttcatctgc taattcacct aatcctgaac ctagaatcaa accaaaatca atttctttta
     1621 ctcctttatc aaggataaac tgggtcgttt cttgtaacat ctcgcttaac ttggtcattt
     1681 gctttctcct atttaaaaag attattttaa taatttcaag aagctttcgc cattttcagt
     1741 cgaagaaaca ctaaagttct cagcaatcgt tgctgaaata tctgaataat atccttgagg
     1801 caatgcccct tgttctttca tttttttact gtaaaccaat aacggtacat attctcgcgt
     1861 atgatcggta ccagtaaacg tcggatcgtt tccgtgatcg gctgtaatca ttagcaagtc
     1921 gtcttcttcc atcgcttcaa gcaattctgg aattcgtaag tcaaattctt caatcgcatg
     1981 cgcatagccg actacatccc gacgatgacc gtataatgca tcaaaatcta ctaaattggt
     2041 aaagctgatt cctgaaaaat cgtttttcat aactttcagc agctggtcaa cgccatccat
     2101 attgcttttc gtgcggacag attcagtgat tccttgtccg ttgaaaatat cgttgatctt
     2161 tccaacagca atcacgtcct ttcctgcttc tttcaaagaa tcaaggactg tgtgtccgaa
     2221 tggattcaac gcataatcat ggcgattgct tgttcgtgtg aagtctcctg gttcacccac
     2281 gtacggacgt gcaatgattc ggccaatcat atacggatca tcttttgtta tatcacgaac
     2341 gaattggcaa atccgatata attcttctaa aggaatgatt tcttcatgtg ccgcgatctg
     2401 aaggacagga tcggcagaag tatatacaat taaatcgcct gttttcattt ggtgttcacc
     2461 gtaatcgtca ataacagctg ttccgctata aggtttgtta catacgactt tacggccaga
     2521 aaattcttcg atttgtttga tcagatcatc agggaatcca ttaggaaaca cacgaaatgg
     2581 agttttgata ttcaatccca tgatttccca gtggcctgtc atcgtatctt tccctacaga
     2641 aacttcttct aatttcgtcg catagccttg atgatctttc actgattcca caccttcaag
     2701 aggacggatc gttcctaaac caagctgctc taaatgtgga acgttcaatc ctgcttcttt
     2761 cgcaatgtgt cctaatgtat cagaaccaac atcaccgaac ttatcggcat ctggtgcttc
     2821 tccaattcct actgaatcca tgacgatcag atgtacgcgt ttaaacatga ttattccttc
     2881 tttcttttta tttttattgc ctttaaaaca aaaagggcga gacgaaaaac acttcccgta
     2941 acccaccctt tttattcgct aaaagattat tttgatttga tatagttctt cccgtttgct
     3001 tttggaccag aagctttccc taagaaaaca accagcacaa tgattgtcag caaatatgga
     3061 gcaatttgca gcaatactgg cggaatcgaa gaaataactg gtaattgtgc gccaatgata
     3121 ctcaagcttt gagcaaaacc aaagaacaat gaggcaccca ttgcccctaa aggattccac
     3181 ttaccaaaga tcatagcagc catggagatg aacccttgtc caacgatcgt accagctgaa
     3241 aagttaccgg aaatcgactg agcaaaaaca gcaccgccca taccgcctaa caagccagaa
     3301 atcagtacgc ctgaataacg catagcatat acattgatac ctaatgtgtc ggcagcttga
     3361 ggattttcac caactgaacg gaggcgcaag ccgaatttcg tcttgaagat gatcacccaa
     3421 gctaggacag caatcaaaat agcacaataa gcaggtaaga atgtattttt aaagaaaatt
     3481 ggtccaatga ccggaatatc tgctaatcct gggaaagaaa aataccccag attctgcgtg
     3541 atgttatcag tttgaccttt accataccat gctttgatca agaaaacacc taaagcaggt
     3601 gccatcagat taattactgt cccgctgata atatggtcag cacgcaaact gactgttgct
     3661 actgcgtgaa tcagagaaaa taggatacct acgacgccac ccactaaaat agcgatccat
     3721 ggtgtcatag aaccaagtga tggactcatc gtcaagttga aaacgatgga actaaaagca
     3781 cccatgacca tgattccctc tagccctacg tttacgatcc cgcttcgttc agaaaaagtt
     3841 ccgcctagtg acgttagaat tagcggtgtt gaataaacca gtgtagaagt aaggatcaag
     3901 gcaattaatt cgatagtcga cattatagtt cgcccccttc atggttttga ttcgttggtt
     3961 tcgattctat tgtcgcaacg atctcttctt gttttttccc ttggaagact ttagccatga
     4021 cgaaacgaat caggaagttg atcccgacaa agaagataat caaagcaatc gatacattga
     4081 cgatctcaaa cggaacacct gctcctgtct gcatgccaag tccaccgatc ttcaaaatac
     4141 tgaacaagat cgctgataaa agaataccga ttgctgatcc agaaccaagc aaggaaacag
     4201 ccattccgtc aaatccaata gatagcgaag tcgtttgaac aaagaagttt tgatacgttc
     4261 ctaatccttc aaccacaccg ccaagccctg ccaaagcgcc agaaatgacc atcgacaaaa
     4321 cgatcgtacg tttactactc attcctgcat actcagaagc aaaaggattc aatccaacag
     4381 aacggatttc aaatcctaaa gtcgttttct tcatcagcag ccaaacgaaa ataagcgcga
     4441 tgattgctaa aaataatcct gagtttacgc gagagccgcc aaaagcttgt gttaaccatg
     4501 gtaaccgcag tgaggcattt tgactgatca tattggaaga gtccaaactt gagcgatagc
     4561 tttcaggcat cacttcatgt aaagcaaatg tgcttacgta taataagata tagttcatca
     4621 tgattgttac gatcacttca cttgttccaa aaaatgctcg aagtaaacct ggaacagccg
     4681 ctgccatcgc accagctaag gcacctaaga tcacgcataa cggaagcaaa acaacttttg
     4741 gcagatctgg aaacgcaagt gccacccaga cactgacgat ccacccgcaa agtgcttgtc
     4801 cagaaagccc aatattgaaa aacccagctg agttagctac agaaaaacct aatgctgtaa
     4861 aaatcaacgg cgttgcttgc cttaacgttt cacctatccc tctttgcgaa ccaagggctg
     4921 catctagcat agaagaataa ccttggatgg gctgaaaacc aaaagcagcc atcaatatcg
     4981 cgcctaagat caatcctaaa acaactgaaa gtacaggcac taataaatta cggaattttt
     5041 cgctgcgatc attcatgtgc gccagccccc ttttctaact cttttcgtgc ttcttctaag
     5101 gtatagcccg ccatcaatag acctaattca ttttcagatg tttctttcgg atcaacgata
     5161 ccgacaattt ctccttcatg gataacagca atacgatccg aaacatttaa aatttcttct
     5221 aattcgaaac tgatcagtaa cactgctttg tatttgtccc tttgttcgat cagacgttta
     5281 tgaataaatt cgatcgcacc tacatccagt ccacgagttg gatttgcaac aatcaacaga
     5341 tcaggatctc ggtcgacttc acgagcgata attgcttttt gctgatttcc gcctgataaa
     5401 gcttttgcgg gaacaagttc attcgttgtt cgaacatcat attcttcgat caattctctg
     5461 gcatgttcat tcattactga ataattcagc atgccgtttt tactgtaagg tttttgatga
     5521 taggtttgta gtgcgatatt ttcagataat gtcatatcta ggactaaccc gtatttatga
     5581 cggtcttctg gcacgtgtcc tacaccatgt tcagtgattt ttcggggttt tttattagtg
     5641 atgtcttccc cttttagctt gatatgtccg ctttctgcct ttcgcaaacc agtcaaagct
     5701 tggatcaact ccgactgccc gtttccatcg attccagcga taccaagtac ttcgccagca
     5761 cgaacctcta agttcaagtt tttcacggct tctaatccac gattttcttt caccactaga
     5821 ttttcaatag acaaaacgac ttcttgaggc ttcgcttctt ttttcatcgt tttgaatgaa
     5881 accgttcttc caaccatcat atcggctaat tgttgtgagg ttacgtcttt aacgttgact
     5941 gtaccgattc cttttccacg gcggataact gtacagcggt cagctactgc tttgatttca
     6001 tctaacttat gcgtaatcaa aatgatcgac ttgccttctt tgactaattc cttcatgatc
     6061 acgattaatt catcaatttc ttgaggggtc aatacagctg tcggctcatc aaaaatcagt
     6121 acatctgctc ctcggtaaag tgtttttaaa atttctaccc gttgttccat tccaactgaa
     6181 atatcacgaa cgtaagcatc tgggttgact gacaatccat actgttcaga aactttttgg
     6241 atctcttttc gtgctttttt atggtcaagc atccctgcgc gactaggctc gcttcccaac
     6301 acgatgtttt ctgttacagt aaaggcatca acaagcataa agtgctgatg gaccatccca
     6361 atccctaatt ggttggcttt ggtcggactt gtgatcttta cttctttccc acgcatcaaa
     6421 attttccctg atgtcggctc taataatccg gaaagcacgt tcatcaatgt agatttgcca
     6481 gcaccgtttt ctccaagcaa cgcatgaatt tctcctgcct ttacttgtaa attgatgttg
     6541 tcatttgcct tgaatgttcc aaattctttg gtaatattct gcatttctat gacaatattt
     6601 tcttgaacca cctttttttc acatcctaac gatttctatc tagggcaacg attgactaag
     6661 attcacgctt agtgagcgtt cttcttttga aacaaacatt acttcaggga gttgttccct
     6721 tatccaaact gtcacaaggc agcttggata aatgaacaat tcttgttcta atcaaaccat
     6781 agccttgtca aacatgtgtc taacaaggct atagaaatgg ttttagtttt cttcaggagt
     6841 ttctggaact tttacgtcac ctgaaatgat tttttctttt gcttcatcaa ctgctttttt
     6901 cgcttcgtca gaaagttgtc cttctgttag accaacaccg tcttctttca atccataaac
     6961 agtgtgttcg ccgccaggga atttaccgtc cgctgatttt tgtgccaaat cttctactac
     7021 tgttccaact gctttcagtg tagatgtcaa tgtgaagttt ttcttttcgc cgtttaatgt
     7081 gtattcgcct tcgtctgatt ggtcgcggtc aacaccgata acccaaactt ttttgtcgcc
     7141 tgattcgttc aatgattttg cttcttggaa tacaccgtta cctgtaccgc ctgaagcatg
     7201 gaagatgata tctgcgtttt gtgcatacat accttgagca attgaacgtc ctttatctgg
     7261 agcagagaag tcaccagcat attgattcaa tactttgatt tcttttccta attctttcgc
     7321 acctgcatca acaccggctt tgaatccagc atcaaaacga tcgattactt caccttttac
     7381 accgccgata aagccgacta cgtttgtttc agtcgtataa gctgctgcaa cacctgcaag
     7441 ataagaagct tcgttgtctt tgaaagttgc agatacaacg ttatctaatc catcgatgac
     7501 atcatcgata ataacaaagt tcgtacctgt attgtttgtt gcttgttctt ggatcgctgg
     7561 ttttagttta taaccaatac caaaaatcgt tttgaatcca gcattcaatg cttggtcaat
     7621 atttgggatg taatctgatt catttgaaga ttggaaatat tggaagccat catttcctcg
     7681 tgaaagtcct tgatctttcc cccatttttc tagtccttcc caagctgact ggttgaatga
     7741 acggtcgtct acaccacctg tgtctgtgat caatgctgca gttgttgtgt ctgatgagct
     7801 tttgccaccg gatgaatcag tagagccgtt gttgccgcat gcacctaaaa ataaccctgc
     7861 tgctagtgcg acagcaccta aaccaaataa ttttgctttt ttcatttctg taaagcctcc
     7921 taaaataatt gttttatttt tcaacagtca gctgactgaa tgaacatgag ttatagatct
     7981 ttttcagtaa atgcataagg cagcagtgca cgaacggtcg ttgccttcgt cacaccattg
     8041 tctcctacta gcgtaaccgg catatcaggg gaacagaatt ctgccattac ttgtcgacat
     8101 gcaccacaag gagagatagg atcgggggtg tgccccgcta cgactagatg cgtaaatgtt
     8161 ttttctcctt cagatactgc tttgaaaaaa gctgtgcgct cggcacaatt cgttaatcca
     8221 aatgatgcat tctcaatgtt gatgccttga taaatcttgc ctgattccgt tacgagacaa
     8281 gcgcctaccg gaaaatggga ataaggaaca taggctttat ctaacgcttc aatagctgtg
     8341 ttgatccatt cttgtttgag ttctgccatg agcttctcct ttgaaaaaat taatagcctt
     8401 caccagttga accagatacg attgctactc ctgcgcttgt tccgattctt gtagcaccag
     8461 cttcgatcat tgccattgct tcttcttcgt tatggatacc gccggaagct ttgacaccca
     8521 tttctggtcc tacagtttca cgcatcaaac ggacatcctc tacctttgct ccgcctgttg
     8581 agaaaccagt tgatgttttg acaaaatcag cgcctgcagc ttttgctatt tcgcaagctc
     8641 ggatgatttc ttctctatct aataacgatg tttcaataat aactttgaca agcgcacgat
     8701 cttttgctgc ttcaacaact gcttcgatat ctttttggac ttgttgatca tcctttgatt
     8761 tcaaagcacc gatattgatg accatatcga cttcatctgc accgttattg atggcatctg
     8821 ttgtttcaaa tgcttttgtc tcgggtgtgt ttgctcctag aggaaacccg attactgtac
     8881 atacggctac tggctcacct tgtaattgtt ctttcgctag cgcaacccat gctggattga
     8941 tgcagacaga ataaaaatgg tatttttttg cttcttcgat aatttgcatc acagcttctt
     9001 tagatgcgtc tgctttcaaa atagtatggt cgatcattcg gtttagttcc atttcttttc
     9061 cccttttctt taaaacggta aagtccgctt ttttccttta ttctgtgatg atgtcatgaa
     9121 tcaacgttgg cttcattgcc gattcaccaa tttcaatatt tttatataat aattcaatga
     9181 cagaagtgat ttcttcgtcg ttactataga tcgtcaatag tgactcacct tttgtcacag
     9241 tgtcaccaat ttttttatgc agtttcaatc caacagcgtg atcaatatct tcttctttgg
     9301 ttttacgccc agcacctagc atcattgcag caattcctaa ttcatttgcc acgatttttg
     9361 acacaactcc gctttgttta gcaggaagct ccattgtgta gcgtgcagtc aaaattcgtt
     9421 ctggatgttc cacgatcgct gggtctcccc cttgattttg gatcatttct ttgaattttg
     9481 ctaacgcttt acctgattcc agagcttctt gaagcaacgt tcttgcttca tcggttgtct
     9541 tagctttgcc agcaagaaca accatttggc ttcctaaggc gtagcacatt tctactagat
     9601 cttctggacc atttccttgt aatgtttcga ttgcctcgac aacttctaga ctatttccga
     9661 tagcttcgcc aagaggttct gacatatctg agataaccgc cactgtttct cggttagcca
     9721 gtttcccaat gctcgtcatg gtctttgcca gacgtcttgc atcttcgatg ttcttcatga
     9781 aagcaccgtc tccagtggtc acatccaaaa cgattgcatc tgctcctgcc gcgatttttt
     9841 tactcataat cgaactggca atcagcggaa gagaatcaac ggtcgctgtg acgtcacgca
     9901 aggcatacaa ttttttgtcc gccggtgcca agtttcctga ttgaccaatt accgctactt
     9961 tactttcatt aacaatgcgt acaaattctt catccgaaag ctcgatctga aatccgggaa
    10021 ttgcttctag cttgtctagt gtaccacctg tgtaacctag accgcgtcca gacattttag
    10081 ccactgtcac tccgacacta gctactaggg gggctaaaat caatgtggtc gtgtcaccaa
    10141 cgccacctgt cgaatgctta tctaccttta ttccttcaag agaactcaaa tcgatgacat
    10201 caccagaatt agcaatcgcc aatgttaagt tcgtgatttc ttcatttgtc atgtcttgat
    10261 aaaaaattgc catcaataat gcactgactt gataatctgg aatctttcca ttcgtatagt
    10321 tcgtgacgat gtactcgatt tcctctttcg acagctcgtt tccatccctc tttttttcaa
    10381 tcagatccac cattcgcatt gcctgatcct ccttgtcttc attcggtatt atacattatt
    10441 tttaggaaag gtaaaccact ttagtaaact ggtttactaa aaataaaaaa agcactaact
    10501 agcctttgta aatgcttaca taagcaatat accgccgagt tagccctttg tcaatacttt
    10561 tacgctctca cgaataatta attttgtttc gaacactttg ttcggaactt ttctttgagg
    10621 aaattcgatc gtatcgatca ggaaacgtgc agcataaaat ccaatatcaa aacttggctg
    10681 ctgcactgtc gtcaattgcg gaatcatgta ttctgacagc tttaatccat cgaatcctat
    10741 gactgagata tcttctggga tttttttgcc caattcatcg atcgcctgat agcatccgat
    10801 cgccatggca tcattcccgc aaaaaatagc cgtgatatct ttatgtttga gtaatttttt
    10861 tgatgcagta tatccacctt ggacagtcaa ctctccatgt tcaacaaatt tcccattaaa
    10921 aggcaggcca tgatctttca gcgcatgacg atatccgttg aaacgttctt ctaattgata
    10981 gtatccagtg gtttccttca gcatgccgat cttcgtatgg ccattttcaa taaacagcga
    11041 aacagcttga taggcgccct catattcctt tacgataagc cgtccgttat cccgacgatt
    11101 caatccgcga tcgattaaaa tgattggcat tttttgataa aaaccgctgt ctaatgcata
    11161 tttctcaggt aaaatatttg gtgtggccag caagattccg tccactgagc ggtgggaaag
    11221 ctcttgtaaa tacttgatct cattttcttg gctatgacga gaattgcata acatgatcac
    11281 atagcccaaa ggattcaaat agctctctac accttcgatc agtttggaga agaagaaatc
    11341 agtgacatca gggacgatca ttccgatggt ctttgaatgt cgattgatca catttgaagc
    11401 gaaataatcg ggcttataat gatgttcatt cacgatcgac aacacttttt ttcttgtctt
    11461 atcactaaat ctgcttcctt tgttattcaa aatctgtgag acagttgtta ctgatacgcc
    11521 tgccatttct gcgatatctc tgatggtcag cttcatccta ttggctccct tctatctaaa
    11581 ttccctatca gtgtaccaaa acccccaaga aaagaataga gaaaaaagga aagtatgttt
    11641 tttgataaaa acctccttta tctaccgact tggcagcctt ctatacaagt tctgtatctt
    11701 atttatttta tcgtaaaaaa caacgactct gttcctacta tttctttttg aagaaaaaat
    11761 agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc cgtacatgca
    11821 cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct ttcacacttt
    11881 tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat gtttcttcca
    11941 ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc agtgtaccgc
    12001 ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt tttccagcac
    12061 ggatcggcgg attgcttacg atagctgcat aggtttcttc atgaactgct tcatagatat
    12121 tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct tgcgctagac
    12181 ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag
    12241 ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca tcaggtaact
    12301 cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt tcacgtgaaa
    12361 atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa gcccattgtt
    12421 caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg tcttccatcc
    12481 ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca agtcaagaat
    12541 aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc tctcttaagt
    12601 ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc
    12661 taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc gagaatttta
    12721 tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa gtttgaatga
    12781 ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc tgatttatct
    12841 ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc ataaatacgt
    12901 agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa aaagtacaac
    12961 agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat
    13021 cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa tcatggaccg
    13081 taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga accgtgtaaa
    13141 atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg atatcattcc
    13201 agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga tcaatacgct
    13261 tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt ctgtctatgt
    13321 cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg cgttactgga
    13381 tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag atcgtgaaga
    13441 agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata
    13501 tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa atcatatcat
    13561 cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat tttgagataa
    13621 aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg aacgttgaaa
    13681 agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg
    13741 atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa gtaaaagcga
    13801 tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat gagaatgcat
    13861 ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt tgctatggta
    13921 tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg
    13981 ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa acaccaaaac
    14041 aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa ggctttgaaa
    14101 cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca cgtaattttt
    14161 atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc
    14221 actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac tttatcgata
    14281 tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag
    14341 gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt gaccaattga
    14401 catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa
    14461 gtctaggcgg aaaattcggc ttgaacatca tcaaagtcaa tgcaagagat cgcttcttag
    14521 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
    14581 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
    14641 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
    14701 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
    14761 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
    14821 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
    14881 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
    14941 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
    15001 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
    15061 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
    15121 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
    15181 ctaaaccacc agcaacagtg gagtgggaat aaagagccaa cataatatgg agggggaatc
    15241 cctataaatc aacattcttc aatagctaaa atgcttgatt ttcattaaaa ataagatggt
    15301 tccctaccaa aagagactat aatataagtt aatttgtttc attcttatca taaaaagaag
    15361 ttctcgaaaa acgaactgtt ttccgagaac ttttttctat ccatctaagt gctcaaatca
    15421 tattcacgac ggaattctaa ggttaccctt tcttaaacaa actttatgtt atctgcacaa
    15481 ctttttgcaa ttttttcatt gcttcttacg aaatcgttga actctcttta gcataataca
    15541 aagtgtagat gaagtctggt gctttactcg ttttggtaaa aatgactgga aatcgtttct
    15601 ggttattttt ggcaaaggcg aggtgcaggt tcgattcctg ccttctacgc taacgatgtg
    15661 gctgaaggga acctccgatc ttgaatcgaa acgatccgca gtctattgtt tcaccaatga
    15721 aacaaaatca acgttcgtcc aagcatttcc tgaagccttg ctataaaccc caatttcaca
    15781 aacacctgtt gtaattggaa tgtctttgat tgtaattttt gtccattcac ttgttttaac
    15841 aaccgcagta tttagttccg ctccaccata atttttgata tataactgat tagtatcaaa
    15901 ctctccccca gacattacat aggcactcaa tgtgtaagtt cctttgctta tgccactaac
    15961 attttgataa gctgatgcct cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc
    16021 aaaaaaagtg cccgcttccg ttttgatcgt acccttagcg gttccttgtt gaaaccaggt
    16081 ttgccaattg gaaggactgg tcgttggtcc atcgctttcg aaacttgcat ttttaactag
    16141 atttatttgc gcccctgctt gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa
    16201 atcaaacatc gcttggttgt cccattcgtt tcccatagtg gattgaacac tgagataatt
    16261 catacctgct tgtgttgccc aactcacatt tccgttgtac caaagaggtt cccaatagaa
    16321 gaagcccgcc ccgcgattat tcggtactgc ctgaatccga tcggcaagat ccagcaaata
    16381 ctctgcttgt cctgtcggtg ttggttcata tccccccgca gctgcttcgg cttcaccaaa
    16441 agcatttggc atagtatcgg cattcgctgt tgtatgccca taagctgttt caactacgat
    16501 cacttcttta ccataccgtt ggctgatcgc attcatattg gtttgaagtt cattcattga
    16561 accgtgccaa tacggataat acgacactcc aattaaatca aatgacacac ctcgactcgt
    16621 gatttcatca aaccaccaag taaatgctcc tgtatcgcca ccatcagcta agtggagcat
    16681 tactggagtt gttttaggtt gagtgtctcg aacagcttgg ataccagact tcaaaaaagc
    16741 agccaatcga tcaaattctc ccccgccttc tccccaagac ttgccataag gccagagcat
    16801 tccgctattc agttcattgc cgatctgaac catatcagga taaacgtcca gctgcttcat
    16861 ttccgacaga acgttctttg tatagtcgta caatgccgta ttcatttctt caaaggtcag
    16921 accttgccat gctttaggaa gattctgttt cccaggatca acccaaaaat cactgtagtg
    16981 aaaatccagc agcaccttaa gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt
    17041 ttccagatca tttgtccctg ctccataact ttctccatta ctattataag gatcctgcca
    17101 caaacgcagt cgaacatagt tgacaccatt attttttaaa atctctaagg catcttttga
    17161 aatatcgtct tcataatatt ttgcaccaga tttttccata tctgacagga tcgaaatatc
    17221 tgctccgcga ataaattcgc tggcagatgc ttcaacagtt tttgaaacag cccccaaagc
    17281 ccctaacaca gctgcaaata aaacagcttt acgcaaccaa attcccattt tcattatggc
    17341 tcctccttta ttgaaattgg ttcagaataa actgaacagc tttttcagga tctctcgtta
    17401 atccgatata ttctgctcct tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt
    17461 ctttgatatc atcatagtaa gaattgactt gcggtgctaa gaccaccata tcaaaatcct
    17521 gcagcatttc gtaatgagaa ccataagcac ccgccattgc actgatcggt gctttcgatt
    17581 cagatgcacc tttttttaaa gcattagcta acatcgcact tgtccctgcg ccagcacaaa
    17641 gtaccaacac attgatttct ttatttgcat cgctgataac ttgactcgct gccacaggta
    17701 cttcctcacg tacatcggca gaagcacctt cctcggttgc gatcacctct gctttttgtg
    17761 cttctttctc tactaagaca gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt
    17821 caacgacgat tagaaccgct gctagaacaa aagctaagat acccatgccc gttcctagaa
    17881 ccaaaccgat cggtgctgga gttgcccaag gcaaaacgta cataaagctg ttcatgccca
    17941 atacatcgac aaaaaacttg aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa
    18001 taaaaaaata agggttcaaa atcatgggtg ccgcaaatag caatggttcg ttgacagcga
    18061 aacttactgg aataaaggaa gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga
    18121 gtaggaagat aaaaggaaca accaatgttg ccccagttcc tcccatagtt cctacaaaat
    18181 tccctaaacc caccgtcagt actttagaag cttgttcacc ggaattgaac aattgcagat
    18241 tggtttccac attggcatag ataattgccg caattgccgg ctccacgatc gacggtccat
    18301 gaacaccgac aaaccaaaac agcgccatcg ctccccagat aatagcgatt cctaagtacc
    18361 cgtcagctgc tgtaaatagc ggctgcagca ccgtaatgat tgcttctgca aatgagattt
    18421 ggaagatttg tcgaatgcct aaatcaatta aaacggccgc aaatactgaa aaagagaaag
    18481 gaaaaatatc tcggaaggtt tgcgaaatcg ttccaggaac ttctttaggc attttaatgg
    18541 taatatcttt cagcacacaa aacttatata aattaactgt taaaaatgca gcgacaaagg
    18601 aagccagtag tccctttgtc cccatatatt ccgttgaaaa tccaccttca atcggagaga
    18661 cgcttaacaa caggaaactt accacagagg ccagcatcac agaggtggtg ttgattactt
    18721 tgtttttcgg taagcgacga ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa
    18781 cgagtaaccc tacaacaccc atcgaataat tatagacttt ccatagccat gtggagacag
    18841 attcaggcca ttggtaacca aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg
    18901 aaaataaaat agctggcatc gctgttaaaa atccgtcacg aacggctcct aaataactat
    18961 tacgtgagat tttttcaaaa aatggtttcc ctttttcgat ttgtttaatg attccatcca
    19021 tagcgttctt ctcctatcct tgattctgtc tgtacagtac aataaagtct tgaatcagat
    19081 ctctcagcaa taaagtggtc attaaatgat cctgcccatg gataaaaatg aatcccatct
    19141 ccatctcttt cccagatgct tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg
    19201 ttaaattttc gtcggcttct ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta
    19261 cttctttcaa taatttcaat aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa
    19321 aaccaagcat ttgtaattcc tctttattca tcttttcttc ctccattatc acttattaaa
    19381 taatcgtttt ggtttcactg accaacttgt accagtaagc tgattctttt gggtaacgct
    19441 tctgggtttc aaaatcaaca taaaacaagc catacctttt gttataccca tttgtccatg
    19501 agaaaagatc cataagcgac cacaaaaaat accccttcac atttaccccg gctgttatcg
    19561 ccttgcttaa ggattccaaa tagactctca aatagtcgat tctcggctga tccataataa
    19621 tgccatcttc aaactgatct ttatacccca tcccgttctc agtgatatag attttgttgt
    19681 aatgtgggta atcgctttta attcgcagca acaagtcata tagaccttct ggataaatca
    19741 gccagtccca atcagttcga ggaattcctt ctttataaat acgttctccg attcccttca
    19801 ctttataaac agaggtccct ttttcacccg taccgttatg atggattgca ttttctccat
    19861 cgtaagcttt gacaaaatgg cattggtagt gattgatccc taagtagtca ttacgcgtgg
    19921 atgctttttt caattcaacg aaatcttctt caggaaaatg atagctggcc tgatttgctt
    19981 cacatatctc atcaagagca gtcaaggttt cagttgaata atagcctaaa taggtagcat
    20041 ctaataagaa acggatcgac aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg
    20101 cgtctgttgc cgcatatttc gtctctaatg agtgaactac accaatttca cccggaagtt
    20161 cgttttcttt gaaatagttc actacacggg catgagcaac catcatatta tgaagacagg
    20221 cgacgatctt tgtaaaatca tatttgattc ctggtgggaa aacacctaac aaatattgat
    20281 tggttgcaac tggatagatt tcattaaagg tactccacac cttgacttct ttgaattcat
    20341 gaaaacaaaa aatggcatag gaaacaaatg cttctattgt ttctcgattc aaaaaatctc
    20401 catggtcaaa caaacgttta ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt
    20461 ttcttttctt acattcttcg aacacacgat gataaaaagc gattccttct ggatttggtt
    20521 tcccagctcc atcaggaaaa atgcggctcc aagcaatgga taatcttaga ccatttacac
    20581 caaaacgttc acacaattca atgtcctttg catattgatg ataaaaatca cttgccggat
    20641 cgggactgaa tcgcccttgt tcctctaaaa agtcatccca agcaacagcg cctttgccac
    20701 cttccttagt ggctccttca acctgatagg ctgctgtggc tccgccaaaa ataaaatctt
    20761 ctggtaattt aagcatgtac atctctcctc aaatctataa tcagttttcg ctctttctaa
    20821 ctgattgcat tgtaaacgca tccaaaacat ttgtcaacaa aaacaaacac aaataatcat
    20881 aaaaaccaga aaacagcaat aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt
    20941 tacttttgtt cgaattagat tgactgttgt ccgtttatat gatagattat ccttaaatta
    21001 gaggtgaaga caatgttgaa aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa
    21061 aaaggaatcc ttaccgttaa tgaaatcatc aatcaattag atgtttctga tatgaccgtt
    21121 cgcagagatt tagatgaatt agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct
    21181 caaagtcttt cctacacttt agatcaagaa ctttctcaca ctgaaaaagc cactctgcag
    21241 attcaagaaa aaaaagaaat tgctgccaca gcagcctcct tgattcacga gggagaaacg
    21301 attttcttag gaccaggtac aacaatcgaa ctattggcga cgcatttact gaacaagaaa
    21361 attcggatca ttacaaataa ttatccagtt tttgatattc ttgctcaagc agatggacca
    21421 gaaattctac tgattggcgg tgatttccgg aaaaataccg gcgcatttgt cggaccgatc
    21481 accaacgaca atttaagaag actaaagttt acaaaagcat ttatcagttc aaatggcgtt
    21541 cacaacgaag cgatctctac ttacagtacc gaagaaggag aagcacagca gatcgctcta
    21601 aataattccc gtaagaaata tctactcgtt gataataaaa aattcaatcg agatgatttt
    21661 tatgtgtttt ataatttgca tgatttcgat ctgctcatta cggatcgcca gatctcgcgg
    21721 gaggttcaag agcactatgc acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat
    21781 accactatag gagcgtaact atgaaaggga ttatatttga tttcaacgga acaatgtttt
    21841 tggactctca tctgcatgaa gctgcttggc tgcatatgat acatgatcat acaaaagatg
    21901 gtttatcaga cgaggatatt ttaaaaaata ttcatggtcg gacgaatacg gagattttga
    21961 aacatttcat ttcagagcat ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag
    22021 aaagctatta tcgtatgctt tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc
    22081 ttgaagcgac tcttgaccag ctgactgacc aacagattcc attaacgatt gccacggcta
    22141 ctgtcaaaga gaatgttgct ttttattttg atattttcga tttagcccgc tggtttgact
    22201 gggacaagat tgtctatgac gacggctctt tccctgggaa accgcaaccg gatatttttc
    22261 ttaaagcggc agacaaactg agcttagctc cggctgattg tcttgttatc gaagatgctt
    22321 actcaggatt acttgctgcc aaacgtgcgg ggatcggaac aattattgca attgatccat
    22381 tcggtaaaaa caaaacagta tttgaacagg cacagctaaa taaagatgga accatcaaag
    22441 attttactgg tttttggaca gagcacctgt ctgccacatc cgttaattct taaacgattc
    22501 aactaactac caaagaaaag caactgaaat gcacacctcc ttttataaaa aactgccagt
    22561 gaaacgacat gaaagataca ctgctatatc cttagtgaaa tatagcagtg tatctttgac
    22621 tttttcattc ttttaaccat atgacgcgtt catctctatc tataattaac attaaaaata
    22681 gtgttttcac attatttctc aaaccaatct taaaaatcat caggagtact tttttgatat
    22741 acttttatta atgctgggcg taatggttaa aagatttcca ctagttcctc tccaccaagc
    22801 tgagttgaaa aatgcttatc tgtaaacaac atagacaaag aggaaataaa cgtttccgta
    22861 tctttctctg tcccaaggat attaattaat tttcctacta ggtaagtatc attaaactta
    22921 agatctgcct tcacatcaaa agttttgata aaagctttta tcatgcgtac aattttgtct
    22981 ttatcgccat aattgatttc accactcata tcaatatagt cactaatccc tgaagaaata
    23041 atggcaactg cttcttcttt cgccttatca gataattcat ttaatgactg ggattgattg
    23101 atgacttctt gagttttatc tgttacgtaa tctctcatta tcctatcggc atactccgca
    23161 aagtaatctt gaaaaaattc ttctacaggg taaccaactt cgttttccca accaggagaa
    23221 atttcattat cagtacttat taaatctatt aaaaaccaac ccgttctctg agtgttgatt
    23281 ctataatatt tttaatttcc tatataaatc aaaaatatta tagaatcaat aagctaatta
    23341 agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt gtgtagctac
    23401 ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac tgcattaaag
    23461 ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg tcaaaatttt
    23521 tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc ttctacttca
    23581 gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga tccttctctg
    23641 taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc tttccactca
    23701 ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt tcccttttct
    23761 accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta catctagtaa
    23821 attttcatca atttctatat cataaagtat ttcactagtg tctacatcca caatatatac
    23881 tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc gtcactttca
    23941 tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag ctctaagcct
    24001 actgtatttt ttactattga gtcattattt accaccataa cttcgtactg tatataccat
    24061 cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt ctccggaaca
    24121 atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc atgttcctca
    24181 cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa aataaacact
    24241 acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc caatcacaaa
    24301 ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac tacaactatt
    24361 catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga tacttgtaat
    24421 agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa ttccaataca
    24481 caacttatcg actattatct gaaaaccaaa acttattatt ccttgatgtt attctagcaa
    24541 tagtaactgc ttttttattt ttttgcatat tttttcggcg ttacttttta aagttaactt
    24601 tttcgacaaa tagaaagaga tacgaatcac tgattttttt gaagaaaaga aatggagtga
    24661 gctgaacgtt agagatattg ggagctttcg taactgctta ttaaacgata atggttattt
    24721 gcaaccgtat tgctcgcttc tacatgaaat atttcatgaa atatttcgca aaacctttga
    24781 ttatgtagtt acgtttccat tcttgactaa aaatgtttca aaaacgaaca aaggtgttaa
    24841 aaggtaaagt gatcaatact actggtcaaa ataagaggtt gaaagggaat tgaaaaataa
    24901 ctacagataa tacttttata aacgtacgtg ctttgtggtt ctgtagttaa ctatataact
    24961 ggaattaggg aatggtatca atttgaaaag aaagattcta ccaatttatc atatgttaaa
    25021 aatggaaaat caacagcagt tctttcggaa accttacaca aaaaccgatt attgaaaata
    25081 gccgaaaaac gtttttcggc tattttttta attaagtttc cttcttctta ccttttccca
    25141 ctattccgtt tcacatcttt gatgatcttc agcgcacgtt ttcgtgtttt gatatttggg
    25201 cttttcaagc ggcggtatgc acttgagata tctgtttttt ccatatggac gacccacttt
    25261 cttctttgat tgtagtcatg ctaaaaaaca caaatcgaaa caattacgat ttgtgtttta
    25321 aatggtatca aaagttaaaa tgaaagtcaa cttgttaaaa cagtcgatca gactagctga
    25381 ttgactccta tctctggaaa taaattttca ttattacgtt ttagctacct aaaaaaggcc
    25441 ctcttgtccg ttaactttaa tatctattgt tatagctagt ttacctattg aacgtactgt
    25501 gtgttcgata gaacac
//
LOCUS       DACEMO010000017        25484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEMO010000017 DACEMO010000000
VERSION     DACEMO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.017"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW728_000534"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW728_000534"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1023915.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IW728_000535"
     CDS             complement(937..1518)
                     /locus_tag="IW728_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1023916.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW728_000536"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW728_000536"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1023917.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IW728_000537"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IW728_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1023918.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IW728_000538"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IW728_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1023919.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IW728_000539"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IW728_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1023920.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IW728_000540"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IW728_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1023921.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW728_000541"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW728_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1023922.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IW728_000542"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IW728_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1023923.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IW728_000543"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IW728_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1023924.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IW728_000544"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IW728_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1023925.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IW728_000545"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IW728_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1023926.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IW728_000546"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IW728_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1023927.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IW728_000547"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IW728_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1023928.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IW728_000548"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IW728_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1023929.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IW728_000549"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IW728_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1023930.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IW728_000550"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IW728_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1023931.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW728_000551"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW728_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1023932.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IW728_000552"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IW728_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1023933.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IW728_000553"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IW728_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1023934.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IW728_000554"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IW728_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1023935.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IW728_000555"
     CDS             13972..14157
                     /locus_tag="IW728_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1023936.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IW728_000556"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IW728_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1023937.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IW728_000557"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IW728_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1023938.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IW728_000558"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IW728_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1023939.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IW728_000559"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IW728_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1023940.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IW728_000560"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IW728_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1023941.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IW728_000561"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IW728_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1023942.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IW728_000562"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IW728_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1023943.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IW728_000563"
     CDS             18232..18879
                     /locus_tag="IW728_000563"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1023944.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IW728_000564"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IW728_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1023945.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW728_000565"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW728_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1023946.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IW728_000566"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IW728_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1023947.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IW728_000567"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IW728_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1023948.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IW728_000568"
     CDS             20317..21255
                     /locus_tag="IW728_000568"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1023949.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IW728_000569"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IW728_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1023950.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IW728_000570"
     CDS             21935..22774
                     /locus_tag="IW728_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1023951.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IW728_000571"
     CDS             22750..23619
                     /locus_tag="IW728_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1023952.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IW728_000572"
     CDS             23609..24406
                     /locus_tag="IW728_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1023953.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IW728_000573"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IW728_000573"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1023954.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8278 a         4701 c         5487 g         7018 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtaggattt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaaggaag aggt
//
LOCUS       DACEMO010000018        24721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEMO010000018 DACEMO010000000
VERSION     DACEMO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.018"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..631
                     /locus_tag="IW728_000574"
     CDS             <1..631
                     /locus_tag="IW728_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1023955.1"
                     /translation="FLRKYKLPVLNPLVLSIVVIILFLHFSGISYKDYNIGGNFLTML
                     ITPATVALAIPLYKNFRLLKENFYPVMAAILAGIVANCLVSVGFGYFFSLHKEMVISL
                     LPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIFGSLIATHIFRLFKIESPVARGVAL
                     GSTSHAIGTAKAIEIGEIEGIISGLAICVNGILTVLLLPLFFQPFAGLF"
     gene            631..2217
                     /locus_tag="IW728_000575"
     CDS             631..2217
                     /locus_tag="IW728_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1023956.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2236..2457
                     /locus_tag="IW728_000576"
     CDS             2236..2457
                     /locus_tag="IW728_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1023957.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2454..3215
                     /locus_tag="IW728_000577"
     CDS             2454..3215
                     /locus_tag="IW728_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1023958.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3595..4104
                     /locus_tag="IW728_000578"
     CDS             3595..4104
                     /locus_tag="IW728_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1023959.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4179..4718)
                     /locus_tag="IW728_000579"
     CDS             complement(4179..4718)
                     /locus_tag="IW728_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1023960.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(4858..5469)
                     /gene="rpsD"
                     /locus_tag="IW728_000580"
     CDS             complement(4858..5469)
                     /gene="rpsD"
                     /locus_tag="IW728_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1023961.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            5785..6582
                     /locus_tag="IW728_000581"
     CDS             5785..6582
                     /locus_tag="IW728_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1023962.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            6610..7245
                     /locus_tag="IW728_000582"
     CDS             6610..7245
                     /locus_tag="IW728_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1023963.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7265..8038
                     /locus_tag="IW728_000583"
     CDS             7265..8038
                     /locus_tag="IW728_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1023964.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8446..9243
                     /locus_tag="IW728_000584"
     CDS             8446..9243
                     /locus_tag="IW728_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1023965.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9221..9634
                     /locus_tag="IW728_000585"
     CDS             9221..9634
                     /locus_tag="IW728_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023966.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            9618..12263
                     /gene="mutS"
                     /locus_tag="IW728_000586"
     CDS             9618..12263
                     /gene="mutS"
                     /locus_tag="IW728_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1023967.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12281..14389
                     /gene="mutL"
                     /locus_tag="IW728_000587"
     CDS             12281..14389
                     /gene="mutL"
                     /locus_tag="IW728_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1023968.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14411..14971
                     /locus_tag="IW728_000588"
     CDS             14411..14971
                     /locus_tag="IW728_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1023969.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15056..17044)
                     /gene="treP"
                     /locus_tag="IW728_000589"
     CDS             complement(15056..17044)
                     /gene="treP"
                     /locus_tag="IW728_000589"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1023970.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17267..17980
                     /gene="treR"
                     /locus_tag="IW728_000590"
     CDS             17267..17980
                     /gene="treR"
                     /locus_tag="IW728_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1023971.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18057..18503
                     /gene="msrB"
                     /locus_tag="IW728_000591"
     CDS             18057..18503
                     /gene="msrB"
                     /locus_tag="IW728_000591"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1023972.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            18673..19275
                     /gene="ruvA"
                     /locus_tag="IW728_000592"
     CDS             18673..19275
                     /gene="ruvA"
                     /locus_tag="IW728_000592"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1023973.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19288..20289
                     /gene="ruvB"
                     /locus_tag="IW728_000593"
     CDS             19288..20289
                     /gene="ruvB"
                     /locus_tag="IW728_000593"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1023974.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20318..20896
                     /locus_tag="IW728_000594"
     CDS             20318..20896
                     /locus_tag="IW728_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1023975.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            20948..21430
                     /locus_tag="IW728_000595"
     CDS             20948..21430
                     /locus_tag="IW728_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1023976.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21547..21921
                     /locus_tag="IW728_000596"
     CDS             21547..21921
                     /locus_tag="IW728_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023977.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(22599..23951)
                     /gene="brnQ"
                     /locus_tag="IW728_000597"
     CDS             complement(22599..23951)
                     /gene="brnQ"
                     /locus_tag="IW728_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1023978.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            24098..24688
                     /locus_tag="IW728_000598"
     CDS             24098..24688
                     /locus_tag="IW728_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1023979.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         8084 a         4473 c         5271 g         6893 t
ORIGIN      
        1 atttttgcgg aaatataaac tgcctgtttt gaatcctctc gtattatcga ttgttgttat
       61 tatcttattt ttgcattttt ctgggatcag ctataaagac tataatatcg ggggcaattt
      121 tttgactatg ctgattactc ctgcgacagt ggcacttgcc atccctcttt ataaaaactt
      181 ccgtttactg aaagagaatt tttatcctgt aatggctgcg attttagcag gaatagtcgc
      241 aaattgcttg gttagtgtag ggttcggtta cttcttctct cttcataaag agatggtgat
      301 ctcattacta cctaaatctg taacgacggc tatttctgtc gatttatctc atacgatggg
      361 cggggtgaat gccgtgacac ttgctattgt tgtctctaca ggtatttttg gttcgctgat
      421 cgccactcat attttccgcc tattcaagat cgaaagtcca gttgctcgcg gtgtggcgtt
      481 aggaagtact tctcatgcta ttggtacagc aaaagcaatc gagattggtg aaatagaagg
      541 aatcatttcc ggacttgcga tatgcgtcaa cggaatattg accgttttat tgctaccgtt
      601 attctttcag ccttttgctg gcttgttttg atgcgaaaac cactatttta tgctacaata
      661 accaaaacgt tcgagcataa aaaaggaggg aaagacgtgt atttgtcttc cgagtatctt
      721 aaacaatttg ataaagatct atattataag attctcctcc tagaaagttt tgaggatcaa
      781 gcttggcata ccgctgcaca attagcgcaa gttgttcagc tggatgcccg cagcgtgtcg
      841 aaatacctga acgagctgtc taaaaactat cagcagttta gcggtaagac acaccctttg
      901 tttacaaaaa atcaccgttc tggttataac ttttatgata cgctcgattc aattgaacat
      961 gagcgttttt tgatctatct cgtgcaaagt acgctgaaat tccagttgct gcacgacatt
     1021 ttttttgaag aattccatac aatgtaccaa tttgcgcaaa agcattacat tagtgaatct
     1081 actgctcatc gaaaaatcaa tgaatggaaa cagcaactgc aaacttacgg aattcggctg
     1141 caacgaggta catatatcgc tcaaggcgaa gaagaaatca ttcgacttta cttgcatatg
     1201 acattctggc agttgtttcg agggaaaatc tggccatttg aaacgatttc tcaaatggac
     1261 gtaaagaata tggcagaaca tatcatggct ttttttaatg ttcgtctgaa cgagatcaaa
     1321 aaaagacgat tggaatatat gctaggtgcc ttttttttaa gaaaatcaca gaagcattat
     1381 gttgtattga atgagaaaaa aagacggctg atttctgaca atctgttgtt ccagcgattt
     1441 tgccaggtca tggagccggt atttcctaat tattttcaag tagaagacga gttaggcgca
     1501 ttatttcttg tactgatgac tcgtgaagaa tattatagtg atcctaagat acggaagaaa
     1561 atttttgatt ttcatcaaca agcaaaaaca ccgcccttta cagccctttc agaagcaaaa
     1621 gccgctcttt ctctttatca ggaagagcag gggctgccag cagaaaatct aaccttcgag
     1681 gcagaaaact atttattcag cagtcatttt ttcgcctatc tttttcctaa tgcgaaagaa
     1741 acgattgatg gcaatagtag tgattttatt aatcatttag ttattgaaaa caaggaattg
     1801 aaacaatggc tcgtacattt ttttgaaagt agacacaaac atcccaatca tctggcattt
     1861 aaaaatcatg cgtttttgat ggggcggtat ctgactgtgt ttaaaacatt aggagccttt
     1921 actccacagc ttccaaagat cacgatcctt ttaatgacag atttcccatt atttgaagag
     1981 caactgttag aggagggatt acggacattt ttccgtaatg agtatcagct gatttttctg
     2041 ccaacagatt atcgggggag agaagttgat cttctgattt cgacaagtaa agtccaccgc
     2101 aaaccatggg cggatcttga ctattttatt gtgactgaag aactaaaatt aattgattac
     2161 atccaactat ctcaaaaatt tgaaatgatt caaaaacaaa aactattaaa acaataacaa
     2221 agaaggagaa aaaagatgac atttgaagaa attttaccag aattgaaaaa aggtgcaaag
     2281 attattcgta aaggatggag tggatttgaa ctttatgttg ttctcgtatc cgatgatgaa
     2341 tttgatggct gtccggtgac gccgtactta ttgatcaaaa caagtgacga aggtttttcc
     2401 agcttcgctc ctacagtttg tgatatttta gcagatgatt gggcgatcgt agaatgaaaa
     2461 accagtatcc agatctagct aaacaggttg tatttatcac aggagctgct tctggaattg
     2521 gtgctgcgca agcccgagcg tttcttgaac aagatgcttt tgtatttgga ttagatcaaa
     2581 agttcggtaa aatggaagaa atttctttgc gatttcctga aacttttgcc tacgcactag
     2641 gtgatgttcg acaaatgaaa gaactccaac aagcggtatc gaaatgccaa aatcattttg
     2701 gtgaagttac gattttactg aatacagcgg ggatattgga tgcctacaaa cccttgatgg
     2761 aaactgacga atcgctgtgg gatctgattt atgagacgaa tgtgaaaagc atgtatcagc
     2821 tgacaaaaat catccttcct gatatgatcc gccaaaaaag cggaacgatc atcaatatgg
     2881 cttcgattgc tggcttgatt gctggtggag gtgggatcgc ttatacaagc gcgaaacatg
     2941 cgattgtcgg atttaccaaa caactcgctc tggattacgc aggacagggt atccgagtaa
     3001 aaggaattgc tccaggagcc atccaaacac caatgaatgc ggctgatttt gaagggaaag
     3061 gtgagatggc agagtgggtg gcaaatgaaa caccagtcaa gcgatgggca cagccggaag
     3121 aagtagctga gctgacgtta tttcttgcct ctcctcaagc aagctacata caaggaacga
     3181 tcatcccaat cgacggcgga tggttgttaa aataaccttg tgtttttttc aagcttctgt
     3241 atgataaaac taaagagaga aacaacggtt gatgataaaa aaagtcgatg actgttcccg
     3301 acattcgatg ccgaacagcc agttcaaaaa attcttttta ataatcgtta atattcgaaa
     3361 ctaagcggct tttcacgacc tcttgatata aacgatattc aaaagcttgc aactgcggta
     3421 acaagtccag ccaaatgaaa aatataagga gctattttta tttgaccatt cttattactt
     3481 gttgcagggc agctttttca ctaccttctg cgacacttca gttggaattt tcttcccatc
     3541 ctttggtcgc ggtcattacc aagataaaat tatcttgcaa aggagaaata gaacatggaa
     3601 aaaaataata cccgttcagc caaacgctgg acaacgacag atgtgactcg gatggcgcta
     3661 gtcactggtt tatatgtggc agtgacgctg gttttgtctg tctttagctt tggagtgatt
     3721 cagattcgtc tctcggagat gttcaactat ctgtccttgt ataacaaaag atacatcgtc
     3781 gcggtaacag cgggagtagc tttggctaat attgcttctc ctcttggact gatagatgtt
     3841 gcagtaggaa gcgtcagcac gctgcttgta ttgcttatca actataaaat cactagtcgc
     3901 atcaaaaaca tgaaaatcaa aatggccgtg acagcatgcg tatttgcttt ttccatgttt
     3961 acagtagctg gtcagctaac gattttatat caactacctt ttttcttgaa ttggtgggtg
     4021 attgctttag gagaattggc atcaatgatt gtcggtggga taattattta ttggatcggt
     4081 aaaaaagtgg atttgtcaaa ataaagatct gtttaattta aaatagggca gcatggaaga
     4141 gactgaatgt tgagtcttct tccatgctgc ttttttgatt aatttgtatg aagaaatcga
     4201 tttagccgtt ttttcatcaa gataccgata taagctgctc cagttgcctt gattgcttcc
     4261 ggcagtaaaa acggcagtag tccactggca aatgcagttg taaaagtaag atcaccactt
     4321 attttcagcc agatcgttcc gcagatcaat gcagcaagag agcccattag attggcaaag
     4381 attgcatata agtaatgagc atccgttctt tcgatcatcc aaccagttat ccacgtttgt
     4441 aagagaaatc caatcaaata tccgccagtt ggcccaaata aaacagctat acctccagtc
     4501 atccctgaaa atacaggaag accaatcaag ccaagtaaaa gataaactcc aacagatatc
     4561 aatcctagtt ttcttccgag aaaagttgct gttaatccaa tagcaaatgt ttgccctgtc
     4621 aaaggaacca aaccaaaagg aatggtcatc tgagaaagga cagaaataat cgcggcaaat
     4681 tccgcagcaa gtatcaattc tttcaatgtt cggttcataa cgccctcttt tcgttaactt
     4741 aatattttta tttggttaac gaaattgtaa aacaaagtga ttactttgta aatagaaaat
     4801 ataaaaaaag accagctatc caatagatag cggtcttttt caagaaagct tttgaaatta
     4861 taagctttgg ttgtagtatt caacgacaag agcttcgtcg atttctggat atagttcgtc
     4921 acgttctggt aaacgagtca atgaaccttc caatttttca gcgtcaaagc ttacgaatgc
     4981 tgggcgtcca acagttgctt ctacagcttc ttttactgtt acgatgtttt gtgatttttc
     5041 acgaactgag atcacttgac ccacttctac gtggtatgaa gggatatcaa cgcgtttgcc
     5101 atcaactaaa atgtgaccat ggttaactaa ttgacgtgct tgacgacgag tcgttgcaag
     5161 tcctaaacgg taaacaacat tgtctaaacg ttgttctagt aagatcatga agttaacacc
     5221 atgtttacct tctttgattt tgctagcttt tacgaacaag ttacggaatt gacgttcgtt
     5281 cattccgtac atgtgacgaa gtttttgttt ttcagttaat tgcatgccgt attctgattg
     5341 tttgccacgg ctattaggtc catgttgacc tggtgcgtaa gggcgacgtg ctaattcttt
     5401 tcctgtgcct gacaatgaga tgcctaagcg acgagaaatt ttccatgatg gtcctgtata
     5461 acgagacatt aaaatttcct ccaatgaata taagtttttg gagtaaaata atccgttgaa
     5521 aaattcataa tcatatagtt tgttcttcaa tcttcacctt tgcagccgcg gttacacaat
     5581 cgaacccgag acggggcaac aaactaggga tgagttatta tttttctgct gcattatttt
     5641 acacaaatta cagtatatga tagatcatcc acgaatgtca agataattag tacataaaaa
     5701 cgctttacaa acctaggctg tctttataaa atgagagggt atgtgcaaat ggaaaaaagc
     5761 gaaggaagtt taaggagaaa atcaatgaat ccggtatcac cattatttga gaaaatcgat
     5821 gcttcaattg aaaagaaggc gaatcttttt gaaaacagct tttcacgtta cgctgttcga
     5881 gcggttctcg cctgtttgtt tttgacttta ggaacggctg tggcatttgc gatcgcgatg
     5941 aagggggaag atatcgcgca tggtctagga aagattttat acgcatttat gttcagctgg
     6001 ggtctggtca tgattctata catgaacgcc gaattaggaa catcgaatat gctttatatg
     6061 accgtgggtg tttttcggca gagacttgct ttaagtaagg cagccaagat tctatttgcc
     6121 tgtatttttt tcaatatgat tggcggtata ttatgtggat ttctaatttc tatgactacg
     6181 ccatttcaag atttaacgtt ggacaacttt atgttagaat caatagcagt aaaactagca
     6241 aaaccttcag caacgatttt ggtggaaggg atgtttgcga atatcgttgt caataccgct
     6301 gtactgatca gcatgcggat gaaagaggat gtcggaaaag tgatcacagt cgtattcatc
     6361 attttcattt ttgctttttt aggatatgaa cacgtgatag cgaattttcc tgcctttacg
     6421 cttacttact ttgcttctca cggacagatg gatggaatga cagtcagcaa tgtgttacat
     6481 aatttgtttt ttgctttggc aggaaattat attggtggcg gtttagtaat gggactggga
     6541 tatgcttggc tgaatcagtc aaaatcatcc tatgtggact aaaaagtcgt tcattaaagg
     6601 ggaagtgcca tggaagtttt agtttttcca aaaaatgagg aaaaagcgtt acaaatggaa
     6661 aagtacatga aaaatctttt ttcctttgct ggagtaccta agccggaacg cgctctttta
     6721 caacaaccgt tactggatga gagcaaaaat ttatctattt ctgaagtttt agcttttgtt
     6781 cattattatt atgaacaaat cgaaagagag tatcagtatg tagcgatcga tttagtccag
     6841 aaaaacatca aacggcttac atttgaagac ttagtcgtat tgttgccact agttcagaaa
     6901 aaagaatggt gggacagcat cgactcttgg agaaaagtat acagcacatg gtgtaaagcc
     6961 tacccagagc agcgtgaaga agtctttcaa ttgtttgaaa aaaaggaaga tttttggcta
     7021 cgtcgaatag caatcacgtt gcaattaggt tataaacagc aaacagacac aggactattg
     7081 atccgagcaa tagacgaaga tcgtcaaaca aaggaattct ttatccaaaa agcaattggt
     7141 tgggcattac gcgattatag taagaccgat gccgattggg taaaaagaca gctgacaaca
     7201 gatttgagta agttagctgt tcgagaggca agtaagtatt tataagaaag cgagacgaca
     7261 agagatgaga cgcaaattat ttgcttttga tattgatgga acgttgttag attcaaacaa
     7321 acaagcttta gatagcacaa gagaagcatt ggaaaaatta cgcaaagccg gtcatctggt
     7381 cacgattgct acagggcgga gccgatttca tgcgcaagag atcattcgcg atcttgattt
     7441 tacgaactac attttatgta atggagcggc cggtttttta gaccatgaac aagtatataa
     7501 gaatttacta gatcgcgacc agttggatgc gttcgttcaa gaagcgcatc atgagcagat
     7561 cgatacagct tttgtcagct tagacaatat caagcgttct tcttccaatg atgtaacggt
     7621 gatggaagaa gcgatgcatt ctttcggtgc tgtcttgcct gaattagatc agttttttgc
     7681 agaggaacaa gaaatatacc aagcgttagc atttttcgat caaacatatg atgggaagtt
     7741 ctcttcatat gataaaattc gatttgtccg ctggcatgag aacagtgtag atgtcgtacc
     7801 taaaaatggt tctaaagcag cgacaattct gcacttagca gaacaagtag gcatccctca
     7861 tgaagacatc atcagctttg gcgatggaca aaacgaccgt gaaatgttgc gcatgtcagg
     7921 aattgggata gctatgggga atgctgtgcc agaagtacaa gcagaagcta agatggtcac
     7981 tgatacaaac gatcaggatg gaatttggaa ggcattgaaa gagttaagcg tgatttaaaa
     8041 gaggttgtga aaaagctgtt ctgcatcaag taataagccc agccaaacaa aaatagctcc
     8101 tcctattttt cgtttttctg ggcttattac cgcagatgca agctttcgaa caccgtttaa
     8161 ggaagaggtc gtgacagaag tggtcagttt caagaaataa gaagggattt acgaaaattg
     8221 cttctcaaat ttttgtaaaa ttacggctta tttctgaaga agtagatgaa acaacgttta
     8281 ttcgggaaat gggactgtga caagatgttt gtcatagtcc cattttttta ttttattttt
     8341 caaaactaag aaataccagg aaagagttcc ttttgtttga ccttttcttt cttattgaaa
     8401 acaactataa tatagagtga tgttaaaaga gaagaggaat ttagtgtgcg ggtattgttt
     8461 attggtgatg tagtaggttc aatgggacga gagatgatta ctgaatatct tcctagattg
     8521 aagaagaagt accgtccgca agtaacgatt gtaaatggtg agaatgccgc ttctggacgt
     8581 ggaatcacag aaaaaatcta taaaaaattt ttgcaagacg gcgtagatgt cgtgacgatg
     8641 gggaatcata cgtgggacaa ccgcggaatc tttgagtttg taaatgaagc taaaaagatg
     8701 gttcgtccgg caaattttcc ggaagggacg cctggacaag ggatggtctt tgtcaaagtg
     8761 aaccaagtcg aactggcagt gatcaacatg caggctcgtt cttttatggt ggacttggat
     8821 gatccttttc gtaaaatgaa tgagctggta gaagaagcca gaaaacgtac gccgatcatt
     8881 tttgtagatt ttcatggaga aacaacaagt gaaaaacaag caatgggctg gtttttagat
     8941 gggaaagttt cagcagtggt cggtacacat acacacgttc agacaaatga tgcgcgaatt
     9001 ttgccaagag gaacagcgta tctgacagac gttgggatga caggtcccta tgatggtatt
     9061 ttgggtatgc gcagagaacc tgtaatcgaa aagtttctga ctgcattacc aaaacgtttt
     9121 gaagttgtgg aacaaggccg atcgatttta agcggctgta tcttggaatt ggatgatacg
     9181 acaggtcatg caaaagaaat ccagctgatt cagatcaacg atgatcgacc gttcatggag
     9241 tagaaagaga ggcagttttt tgcagcagga gccggaaatt caaaaagaag taaggaaact
     9301 gacaaaactt ttaagagaga atgaaacgat cattcgctac aaagaacttg aagaaaagat
     9361 ccagcaaaac cagtatttag ctgagttgag agaaaaaatc aaacaagcgc aaaaagatgc
     9421 tgttcatttt gcccactatg acaaaccagc agcagaaaaa gaagcaatca aacaagcgga
     9481 tcaattcatg caagaatttg atcagcatcc gcttgttgta gcttatagaa aacagctgtt
     9541 ggaagcagat gatctgcttc atcatttaac aactatgata caagaagaaa tcaatggaca
     9601 aatagaggag gaaaaacatg cctcaaaaaa ctaaaaatac tcctatgatg gaacaatatc
     9661 taagcatcaa ggcccaatac caagatgctt ttttgttcta tcgtttagga gacttttatg
     9721 aactatttta cgaagatgcg atcaaagcgt cacagctttt ggaattaacg ttgacgagca
     9781 gaaatcgaaa tgcagaagaa cctattccta tgtgcggtgt acctcaccat gcagcacaag
     9841 gatatatcga tgccttgatt gaaaaaggct ataaagtagc gatttgcgag caggtagaag
     9901 atccaaagac gactaaaggg atggtcaagc gcgaagtgat ccagctagtc actcctggta
     9961 cagtcatgga cagcaaagga ttgtcggcaa aagacaacaa ttttttaaca gctgtagtta
    10021 gtcaaggaaa tgaatttggc tttgcttatg ccgatttaag tacgggagaa ttgaagacgg
    10081 ctcggttgca tgatgaagaa gctgttttaa atgaagcctc tgcattgcag acaaaagaac
    10141 tcgtattagg cagtgagatc actgataccc ttcgtgaaca gctaagcggg cgtttagggc
    10201 tagtcttttc tcaacaaaat gagatggaag aaaacgcgga atttagcttt ttgacaagcg
    10261 aactagtcga tccgctggaa aaagaagcaa ccggcaaact attgacttat ttatcggtga
    10321 cacaaaagcg gagtttagct catatccaaa aagcagtaga ataccaacca gatcattttt
    10381 tgaaaatgga ttattattca aaattcaacc tagaattaag ccaatctatt cggactggga
    10441 aaaagcacgg cactcttcta tggttgttag atgaaacaaa aacagcaatg ggtggccgtt
    10501 tactaaaaca atggctagac cgtccgctta tccaggcaaa acaaattaaa gcaagacaag
    10561 aaatggtcgc ttctttgcta gacagctatt ttgaacgtat cgatttgcag tcagcattaa
    10621 cgaaagtcta tgatctggaa cgcctagcag gtcgagtagc tttcggtaat gtcaatgggc
    10681 gagacttgat ccagctgaaa acttctttag aacaagtgcc aatgatcaga gggttgattg
    10741 aaggaatcga tcaaggtcaa tggcaaagtc tgctttcaga aatgcagccg atggaccatc
    10801 tcgtccaatt gatcgaccaa gctatccaag atgatccgcc gttacaaata acggaaggaa
    10861 acatcatcaa agatggattc aatgagcagc tcgatatgta ccgttctgct atgagaaacg
    10921 gaaagaaatg gttggcagaa cttgaagcaa aagagcgtca agaaacagga atcaagaact
    10981 taaaaatcgg atttaaccgc gttttcggtt attatataga aattacaaaa gcaaatcttg
    11041 gaaatgcaga actggagaag tatgaacgaa aacaaacttt agcaaatgca gagcgtttca
    11101 tcacaccaga actaaaagaa ttggagacac aaatcctcga agcagaagaa aaatctgttg
    11161 atttagaata tcagttgttt ttagctgtga gagaagaagt caaaaaggct atccagccgc
    11221 tacaagtact tgcaaaagca atcagtgcag tagatgtgct acaaagtttt gcaacgatca
    11281 gcgaacgcta tcagtatgtt cggccagaac ttgtcagtga caagcatcag cttctgatca
    11341 aagacggacg acatcctgta gtagaaaaag tattaggaca tcaggaatat atccctaaca
    11401 gcgtagaaat ggcagaagac gaaatgatct tgctgattac aggaccgaat atgtctggta
    11461 aaagtacgta tatgcgacag ttggcattaa ctgttctgat ggcgcaaatg ggctgtttcg
    11521 ttccggcaaa acaagcagtt ttacctattt ttgatcggat ttttacccga attggtgcaa
    11581 gtgacgactt aattgctgga caaagtactt ttatggtaga gatgatggaa gccaatcaag
    11641 ccctgcgtta tgctacgcca aacagtttga ttttgtttga cgaactaggg cgaggaacag
    11701 caacctatga cggaatggcg ttggcccaag caatcatcga atatatccat cgtcatgtac
    11761 aagcaaaaac actcttttct actcattatc atgaactgac ggtacttgag aaagaacttc
    11821 cgcagcttaa aaatgtccat gttggagccg ttgaaaaaga cggagaagtt gtcttcttgc
    11881 ataaaatgat ggatggacct gcggataaaa gctacggcat acatgtggca aagatcgcag
    11941 gattgccttc caatctacta gaacgagcag cagatatcct gcatacgcta gaaaatggcg
    12001 ataacggaca ccatactgtt ccagctgaag tagaagagaa aactaaaaca gccaaacagg
    12061 agcaaataaa accacctgca gaaaaagtaa atcaagacca tatacaagac cgtgtaaaag
    12121 aagaaaacca acagctttct ttattcagtg aattatccga aaatgaaaca gaagtcattg
    12181 aatcactgaa gcaaataaat ttactagaaa tgacgccaat ggatgcacta aacacattat
    12241 atgaattaca aaaacaactg taggaagaag gcggatatcg atggccaaaa tccaagaatt
    12301 atctgaacgt ttagctaatc agatcgctgc gggagaagta gtggagcgac cagcttccgt
    12361 tgtgaaagaa ttagtggaaa atgcaattga cgccggcagt actcagattg atatcttagt
    12421 tgaagaagcg ggactgcgga cgatccaagt gattgataac ggagaaggca ttttggcaga
    12481 cgacgtagag aatgccttca agcggcatgc gacaagtaag atccataatc gcgacgatct
    12541 ttttcgcata cgaacgctgg gctttcgagg ggaagctttg ccaagtattg cctctgtttc
    12601 tgaaatgact ctggaaacgg cgaatcagga agaaaaacaa ggcacctatc tgtcgctgaa
    12661 aggcggagaa gttattgagc atcgaccagc accactgcgt caaggaacga agattaccgt
    12721 cagcaatctg tttttcaata cgccagcccg cttgaaatac gtgaaaacac tgcacacaga
    12781 attagcgaac atcggggaca tcgtcaatcg actggcattg agtcatccat cgattgcttt
    12841 tcgtttagta catgatggaa acaaaatgct ggcaactgct ggaaatggcg atctgaaaca
    12901 aacaattgcg ggaatctacg gattatctac cgcaaaaaaa atgctgaaaa tcgaaggaaa
    12961 agatctagat tttgagttat ttggctatgt ttcattgcct gaggtgactc gtgccagtag
    13021 gaattactta tcgacgatca tcaatggacg ttttatcaag aacttttcct tgaataaagc
    13081 aatcgttgca ggttatgggt caaaattaat ggtcggtcgt ttccctattg ctgtattgga
    13141 aatcaaaatg gacccgcttt tagtagatgt aaatgttcat ccgacgaagc aggaagtccg
    13201 gctgtcgaag gaaaaagaac taacagagct aattagtaac gccattcaaa aagcattgtc
    13261 tcaagaaaat ctgattccaa gtgcagcgga taatctacgg tttaagaaaa aagtacctga
    13321 tcagcctaaa acagaacaaa tcgaaatcga tttgtcctac gcttttgaag aagaaaaacc
    13381 taaaaagcca agtagtttga attttgatcg agagacaggt acctttttcg tagaagaact
    13441 aaagaacaca tttgttgaca gaattgttga taagtctgtg gaaaatgttg aaaactattc
    13501 tggtttttct gaagaaaaca ccacttatcc agaaaaaaca gcgaaaacgg tggataagtt
    13561 tggtgaaaat gttgataact ttgtagaaaa aacagtggaa aaatctgaaa agaaagaatt
    13621 tcccattgtg gataacatgg agaaaacaaa tgaagaacaa gcagataaat ccgtagaaga
    13681 ggatgaaaag atttacgagg aagaattgac actccatcct gaatttgatt tcactcaagg
    13741 aaacgacaaa acattagaac aagccatcaa gaaattagaa acggaaaaac cagtacaacg
    13801 ttttcctcag ttggaatatt ttggacagat gcacggaacg tatctttttg cccaaagtag
    13861 cgatgggttg tacatcattg atcagcatgc tgcccaagag cgcattaagt atgaatattt
    13921 ccgggaaaaa attggggaag tatctgatga tctgcaagaa ttgcttgttc catttgtgtt
    13981 agactatcca aacagtgatg ctttgaaatt aaaagagcaa aaacacttgc tagaagatgc
    14041 agggatttat ttagaagaat ttggtcaaaa cagctttatt gtacgggccc atcctacttg
    14101 gtatcccaat ggagaagaag aatcgattat tcgggagatg atcgatatgc tgctgacaac
    14161 tggaagtgtg agtgtaaaaa aattccgtga agcaacagca atcatgatga gttgcaaacg
    14221 atcgattaaa gccaaccatt atttgaatga agcacaagca cgtgtgctgc tgaaggatct
    14281 ggcacgatgc gaaaatcctt tcaattgtcc ccatggccgt ccagtattga ttcactttac
    14341 caattcggat atggaaagga tgttcaaacg aatccaagac ccgcattaag tcaaagagaa
    14401 gagggaaaac atgaagattg tattagcatc ccaatcacca agaagaaaag agttattggc
    14461 tcatatcgtt tcagaattcg agattgaacc tgctgatatc gatgaaacgc catttccaga
    14521 cgaacagccc gtcgattatg ttcgtagaat ggctgaagaa aaagcacgaa ctgtctggga
    14581 acaagatgaa aaagaagacc atttagtgat tgccagtgac acgactgttg ttctcaatca
    14641 agagatcatg ggtaaaccag aagatctggc tgaggctgaa ttcatgctga aaaaactaag
    14701 cggggaaaca catactgtat atacagctgt tgttttaaaa acaaaggaac gagaagaacg
    14761 gatactagca gaagcacatg tcacttttta tcctttgaca gacgaagaga tcaaacgcta
    14821 cttggagacg ggggattatg cggataaggc aggggcatac ggcatccaaa atgctgcgag
    14881 tgtattcgtc aaagaaattt ctggagatta ttacagtatc gtaggatttc cgattggtgc
    14941 tgtcaatcag gcactgaaaa cattcattta aacaaaaaag cggactatct gattttcgat
    15001 tgaaaatcca tgatagcccg ttttttttat tgaaaagatt acttatctac tctgactaag
    15061 attttacttc agctttaccg actgcttcgc ccgggcgaac aagtcctgat tggtcaagag
    15121 aaagtcctgc aactttttca ttattggtca aagcaacaat aatgtcagtt ttcttgccag
    15181 cttgttctat ttctggcaaa tccatttcag caatcagggt ctctggtgtg acaaggtatc
    15241 cttctttcac gaatacattg aaaggaacac cctttagttc aacagtatct aatcccatgt
    15301 gaacaagtac ttccagcccc tcattagaaa gaataccaat ggcatgtttt gtttcaaaga
    15361 cactcgtcac tttaccgttt actggtgcat agactttgcc gtttgacggt tcgacagcat
    15421 aaccgtcacc catcattttt tgtgagaaaa ctgggtcatt tacttcagtt atttccttga
    15481 tggtccctgc agcgactgcg aataatgttt cttttgttgc aactgccgta ccagttgctg
    15541 aaacaggttc gaaagactgt ggcgttgctg ttgcgccatt tgctgtttga agttgtacat
    15601 cagcagcatt ttcaggaaca gcaggatcaa tcttattcaa aattccctga cgtctaaaga
    15661 tgatcgtcaa gccaaatgga atgatgacag caatgatcat tgcgatgatg aaaggtaccc
    15721 atgtttctgc ttggatagct agtattcctg gcaaaccgcc tactccgatc gcgttagctc
    15781 gaacacccat caagttagca aacatgccag ctaatccaga gccgaccatt gcagcgacga
    15841 aaggatagac gtatttcaaa ttgattccga acatagcagg ttcagttacg cctaagtaac
    15901 aagaaatcat tgctggaatc gagatttgtt cttctttttt attcccacga tgtaagaaga
    15961 tgatggctaa gactgcagag ccttgggcga tatttgataa agcaatcatc ggccataaat
    16021 tcgttgatcc gaaatctgca atcagctgca tatcgatggc attggtcata tggtggagac
    16081 cagtaatcac taaaggagca tacaggaatc cgaatacggc actaaacaac cagctgatag
    16141 aagacgtcaa tccagtattt acgatatttg agatccagct tccgactgtc cagccgatag
    16201 gacctaaaat aacgtgtgca gctaaaacag ttggtaacaa tgaaaacaat ggaacaaaaa
    16261 tcatcgagat cgactgaggg atatatttgc ggaagaagat ttctagatac gctaacataa
    16321 accctgccag catggctgga ataacttgcg cttgataacc aatcatctgg acttgagcaa
    16381 aaccaaaatc ccaaaatgga atatctgctg cggctgtgga agctacacta taagcattta
    16441 ataattgcgg agaaactaac gtaattccta aaacgattcc taatatttgt gtagtaccca
    16501 tcttttttgc aatactccaa gtgataccta ctggaagaaa atggaaaatt gcttcaccgg
    16561 gtaaccacaa aaaggcattg acaccattcc aaaacttcga gtgttcaacg attgttccac
    16621 caagactttc aaactggata ccttcaagta catttctgaa tccaaggatc aatccaccga
    16681 caatgattgc aggaatcagt ggtgtaaaga tttctgctaa aacggcaata gcacgttgaa
    16741 cgggatgaag gttttgtttt gcagctgctt ttccttgttc tttggaaacc ccttcaactc
    16801 cagatactgc aacgaagtcg ttataaaaag tagaaacatc gtttcctatg atgacttgga
    16861 actgtcctgc attcgtgaac atccctttta cgctaggtat atcttcgatc gctttttcgt
    16921 ccgcttttcc tggatcattc aggacaaaac gcatacgagt cgcacaatga ctaactgcag
    16981 caatattttc ttttccgccg atatcatgta acagcttctc ggcatctgct tgatattttg
    17041 ccatatcaat aaaccccttt ctcttaactt gtctatgcaa gttgtttctt catttactat
    17101 actttttttc ggagagaatt gcaagcgttt tattaaaaag tacgagagaa aatgatatcg
    17161 ttttaaaaag cttaaaataa gcttttctta aaaataggaa cattgaaata caaaaagagc
    17221 tgtagtatag tgaagaaatg atggaagaac gagtgaggga aaaaacatga ataagttcaa
    17281 tgaaattttt ttagatttgg aacagaaaat tttagcgaaa gaatatcctc cgcattcgct
    17341 tttacccagt gagaaccaat tgatcaaaat ttatggtgtt tcacgagaaa caattagaaa
    17401 agcgctgaat cttttgacta cggaaggtta tattcaaaaa aaacaaggaa aaggttcgat
    17461 cgtactggat cggaatcgct tcgactttcc tatttctgga ttgactagct ataaagagct
    17521 acaggagacc caacgtattc caagtgaaac gatcatccat acgttagaag aaacagaagt
    17581 gacaaaagca ttgaacgaga tcactggctg ggagatcggt gcgcctgttt ggcatttgat
    17641 acgcgaaaga aaaatcaatg gcgaagtagt tatattagac accgattatc tattaaagga
    17701 aatcgttcct attttaactc cagcacaagc acaaggatcg atctatgaat attttgaaaa
    17761 tgagctttct ttgacgattg cttacgcgca aaaggagatc acagtagaag aagtaacgga
    17821 aatcatgaaa acagcgatgg atcttcacga tgacacttat gcagtagtag tcagaagcct
    17881 cgttcatctg gaagataccc gctgtttcga gtattctgag tcggttcacc gattagataa
    17941 attccgtttc gttgagtttg cccgacgccg aaaagtgtaa atagttcaaa agaaagcaaa
    18001 ttgttggcat ctgtccactt tggcgctatg ataggatcac aaagggggca tttttaatgg
    18061 ataaaccaat aagaccagaa aaagaagaac tgaaagaaaa gttatcaccc atggcttatg
    18121 cagtcacaca agaaaatgca acagaacgac catttagcgg gaaatatgat gatttttacg
    18181 aaaaggggat ctatgtagat atcgtcagcg gtgaaccgtt attcagctct gcagaaaaat
    18241 atgatgcagg ttgtggctgg ccatcattca gcaaaccaat aacgaaaaga caagtgcgcg
    18301 aaaaagccga tttttctcat ggcatgcatc gaatagaagt tcgaagcaaa gaagcagatt
    18361 ctcatctcgg acatgtgttt accgatggac cagtagatca aggtggtcta cgttactgca
    18421 tcaattctgc ctcactgaaa ttcattccat atgaaaagat ggatgaatta ggttatgggg
    18481 aattcaaatc acttgtagaa taatggaaat acacaaagag cggtacaata cggggattta
    18541 ctcgatcgtc cgctcttttt ttattcaagc gattgctatt tgaaagaaac gctccctaga
    18601 ctcctttttt cacaaggtga aatgtgctac aataaaggga acgtatgtgt gcaaaagaaa
    18661 gaggactaga atatgtatga atatctgaca ggtgtggtta cctttatcaa cccctattac
    18721 atagtagtag aaaccaatgg tatcggctac cagatcgcac taggaaatcc ttaccgctac
    18781 agtagcaaac taaatcaaaa aataaaaatt tatgtccatc aggtaatacg tgaagatgcg
    18841 catcttctgt atggatttga ttcgctagaa gaaaagcaac tgtttttgcg attagtaagt
    18901 gtttcaggaa tcggtccgaa aagtgccctt gcaatcatgg caagcgaaga ccactccggt
    18961 ttgattcaag cagtagaatc cagtgatgtc acttatttga cgaaatttcc aggagtaggg
    19021 aaaaagacag cacaacaaat gatcttggat ctaaaaggaa aattcggtga gctgagtatc
    19081 gatacacctt ttagtctatt tgatgaagca aacacaaaag acgctacagc tctatctgaa
    19141 gcaatggaag ctctctcggc tcttggttac agtgatcgag aaatcaaacg tgtagaaaaa
    19201 caattaaaag aagtagataa tcagacaacg gatgagtatc tacgccaagc actcaagcta
    19261 atgatgaaaa aataaggagg agaccaaatg acagatgaat acttgttatc accagaatct
    19321 ggcgaaaatg aactgagttt ggaaaaatct cttcgaccac agctgctttc tcaatatatc
    19381 ggacagagaa aagtcaaaca agaacttcag atttacatcg aagctgccag aaatcgggaa
    19441 gaacctttag atcatgttct gttgtacggg cctcctggat taggaaaaac gacgatggcc
    19501 atggtcattg ctaatgagat gaccgtaaat attcgtacga cgagtggccc tgctatcgaa
    19561 cgtccgggtg acttagtagc aatcttgaat gaactggaag caggggatgt cctgtttatc
    19621 gatgagatcc atcgattacc tagagtggta gaagagatgc tttattcagc tatggaagat
    19681 ttctatgtag atatcatggt aggacaaggg ccaaccgcac atccggttca tttccctctt
    19741 cctcccttta ctttgattgg cgcgacgaca cgggcaggta tgctttctgc acctttacga
    19801 gatcgatttg gcatcatttc tcatatggaa tattatgacg aacaggatct gcaggagatc
    19861 gttttacgtt cttcggatat ttttcaaaca gagatcattg aagaaggggc attggaaatt
    19921 gctcgccgat cacgaggaac gcctagaatc gctaatcgct tgctcaaacg ggttcgtgat
    19981 tttgcgcaag tccaaggaga cgggaaaatc gatcgtccta tagcggatca ggcacttact
    20041 ttgctacagg tcgatcaggc tggattggat tatgttgacc agaaattgtt gcgaacaatg
    20101 atcgagttat atggcggcgg accagtaggg ttaagtacgc tttcagtaaa cataggagaa
    20161 gagcgtgaga cggtagaaga tatgtatgaa ccgtatctga tccaaaaagg atttttaaag
    20221 cgtacaccta gagggcgaat tgctactgct tatgcctatg aacattttgg ttacgactat
    20281 catatatgat ttttgctttt agattggagg aaaatgaatg agtgatacaa cacgtacgaa
    20341 acaattgatc aaaaacgtat tgattgattt gtgccaaacg caatcttttc gaaaaatcag
    20401 cgtccaaaaa atcgcaaacg aagcaaaaat caatcgacaa acattttatt atcattttac
    20461 agataaatac gatttgcttc gttgggtcta ttgtgaggat gcattgcatt atttagatcc
    20521 acaatgtctt tcacttgata attgggaaga gcaagcattg aaaatgctga aagcaattgc
    20581 tgagaagaaa gaattttatg caaatactgt gctttctgac ccagaaattt tacaaaaaga
    20641 gttttctcaa ctgatccaaa aatcatttat caagattttt gaacaaatgg atgaagaaag
    20701 acaactttct gcaagagaca aactatttta tgctcgtttt ttttcttacg gttgcagtgg
    20761 tgtattagtc aattggatca cgaatgaata tgaagaaaca ccgatggaaa tcgctatgca
    20821 gcttttccgt ttagccaagg atacggaatt atattcttat cgtctttatg caaaagaaga
    20881 gttggaagaa cagtaataga aaaatttttt gttggaaaag caataatcgc taaaaggaga
    20941 tggggaaatg acaaaaattt tatttgtttg tttgggaaat atttgccgtt caccgatggc
    21001 agaaggcttg ctgagaaaac gaatagcatt agaaggaagg gaagcagaat tttttgtgga
    21061 ttctgctgct acaagcacgt atgaagttgg aaagacaccg caccctggaa cgaaaaaaat
    21121 attaaaccaa gaaaaagtag atatgacgaa tatggttgct cggcaaatca caccccacga
    21181 ttttgaaact tttgattgga tcattggcat ggatcaagaa aatgtagaag aactgaaacg
    21241 aagagcacca cgctcagcgc aggggaaaat ccatttgttt ttaagtagca ttcctggaaa
    21301 agaaaaagaa aatgttcccg acccatacta taccaataac tttgatgaga catatcactt
    21361 gatcacagaa ggcctgaatc attggtggaa tatttggatt gatgaaaata aaataacaaa
    21421 caatttgtaa agttaacgct ttctttttaa ggcgatgtta gagcatttcc ttgttttttt
    21481 gtaaaatgtt aggtaaaata aaattaatta agtaacactt tcttagctaa aggaggagtg
    21541 cattcgatga acgaagagac agtatcgcca atgaagaagc ttacgaagtt agttactcat
    21601 acttcgtatc aactttttga ttcttttgta aatgacactg aagaacatgc taagaatgta
    21661 cgattgcaag gaaagtttat tcttgagctg caaaaagctg ctactgaaca tagtctagtt
    21721 atcttacaag taagagcaaa tcgtttgagt agcaagtatg aaacgatctc aggctgggtg
    21781 gcgacaaaaa cggtcgaaaa agaccgagtg atgatccgcc tacaaaacga agagcagcag
    21841 atccggatgg ttcctgtaga acaaattgaa aaaataacaa tcctttcacc agcaggggaa
    21901 caggaacggt tatcaaaata atttatacca gatagaaaag cacgtatgag cttgtaccat
    21961 caatcattac taatcaaaaa taaccgaatg ctttaagctg atatcagtaa aacggaacgt
    22021 tttaggatga gtatcacagc atgacagcat tgggttgatt agttgaatcg attggatggt
    22081 acaagttttt ttgataaaaa aaggtcctgc tagccgggga gctagcagga taaggagtta
    22141 aaaatgaaaa agtgttgtta gggttgtttg ttggtatgct tatataatag tcactagttg
    22201 tgaatatttt gtgacgaaag ttttcgaaag ttgttaagtt tttttgcgaa aactattttt
    22261 atatcatata tatgtctaaa atctatatta taaaagaaat gttgtttgat cagcttctct
    22321 tcggtattag ttcaaaacaa aacggcaaag tatatggagc tactttgccg ttttgtttct
    22381 ctaatactca ggtcagttta tagacgctca ataaacggtg ttcaaaagcc agtttctcgg
    22441 tattaagtca aatttatgca aaaatgtgag aagccatttt caaaaaatat ttcttaatac
    22501 tcgaaactga gcggcttttt cacgacctct caataaacag tgttcaagag acagcctatc
    22561 ggcaataagg ctgaacgtct ctttcacaac ctcttttatc aatctgctac ttcttctttc
    22621 ttagcgaaac tccaaatcag tccgataaca aaaccgataa ctgctggtac gatccacccc
    22681 atccccaatt tgaaaaacgg caagtagctt tccgcaacat ataaaagatt ttgaacaata
    22741 ggtgtttgtt tgatagtttc tgggcaagcg ttcaacccat caaaaatcga agcaatcaat
    22801 gtaaaataag tagtcattcg ataaacagcc gtacgctgct taaacaaagg accaactaac
    22861 actagtaaaa tcagtgtcat tgctaatgga tagatgaaca tcaaaacagg caacgaaaat
    22921 tgaatgatat tcgtcaagcc tacgtttgcc gctaaacaag gaaggatact agcaagtgca
    22981 acaaagaatg cgtaatttct ttgcggaaac aaatctgtga atgtttctgc aaaagctgta
    23041 atcaaaccaa taccggtctt caagcaagct aatataacga taaatgctaa taagatactg
    23101 ccataagtac ctaaataatg atcagcaatc tgagccagcg tgatcccgcc attttcactt
    23161 agagcaaagc tacctaagct catagcacct aaataagcga gcaaagtata aatgattccc
    23221 atcaatataa tgctgatcgc accagatttg atcgtgtctt tagcgatttc agcaggtttt
    23281 gttacaccca tgttttggat tgttgtgaca atgatgatcc caaatgctaa agcagcaagc
    23341 gcatctaatg tgttatatcc ttgagtaaac cctgtgaaaa aagcatgctc ttgataattt
    23401 ggctgaatag gcgcttgatt tacagaacct agtggattca aaaaagcaag gagaagcaaa
    23461 gcacctaata aaaccaaaaa tgcaggattc aggaactttc caacataatc taatatcttt
    23521 gtcggttttc ttgaaaacca ccaagccagc acaaaaaata caatactgaa aattgctaga
    23581 tatagcgtct gtctgctagg atcgataaat ggtgctaaac caatctcgaa agatgtggtt
    23641 gcaaggcgag gtaaagcaaa aaacggtccg ataactaagt aaagcaagat ggtaaaaacc
    23701 atcgcatatg tttgatttat ccgttttgct aattcatata cacctgagct tttcgataca
    23761 ccaatggcaa tcacccctaa gaagggaaga ccgataccgg taaccaagaa acctagattc
    23821 gcccaaaagg tagcagcgcc tgcttcttga cccaaatgaa cgggaaagat aagatttcca
    23881 gcaccaaaaa atagaccgaa aagcatggag cctataaaaa gatagtcttt aaatgataat
    23941 ttttttgtca tacataactc ctttacggtt tttttaaaag taacaaaatt tcgaaaaata
    24001 tgcaacaatt ttcagaaaat ttcctatata aaacgacggt tatttaataa cgtaagtgat
    24061 aaaataaaag cgctgaatca tctgaggagg aatcatcgtg acaaagtcga gattagaagc
    24121 atttactgat ggagtggtag ccattgtttt gagagttctt gtattagaga tcaagatccc
    24181 taatacatct agctttcatt ctttatgggt catccgaaat acattattag cttataccat
    24241 cagctttata tttgttgcgg tgatctgggt gaaccatcat cgtatatttc agatggcaga
    24301 acgaattaat taccgagtgg tctggtcgaa tatctttttg ctcttttggc tgactttatg
    24361 tccttctgtt actagttggg tcgggcgtaa ccctgaagct ttttggcctg agatggctta
    24421 tgtcttagta tacacgatgt ggagtttttc ttttggtatc ttgatgaggc aaatcatgaa
    24481 agcaaataaa ccagattccc atgtcgtgag agttttgtca aaggaccgtc gaagtcttat
    24541 ctccatgatg attaacttat gcttgattgc cggtgtcttt tttgtaccgt tgatcgggtt
    24601 attcggacgt ttttttgtat ctggtatttg gattttttcc tataaaaaag cagatgccta
    24661 ttatcagcgg ttgtttccgg ggaaatagaa aagaggctgg gacagaagct tttaactcca
    24721 a
//
LOCUS       DACEMO010000019        24395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEMO010000019 DACEMO010000000
VERSION     DACEMO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.019"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(58..603)
                     /locus_tag="IW728_000599"
     CDS             complement(58..603)
                     /locus_tag="IW728_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1023980.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(615..1307)
                     /locus_tag="IW728_000600"
     CDS             complement(615..1307)
                     /locus_tag="IW728_000600"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1023981.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1326..1610)
                     /locus_tag="IW728_000601"
     CDS             complement(1326..1610)
                     /locus_tag="IW728_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023982.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1623..2186)
                     /locus_tag="IW728_000602"
     CDS             complement(1623..2186)
                     /locus_tag="IW728_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1023983.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2414..3076
                     /locus_tag="IW728_000603"
     CDS             2414..3076
                     /locus_tag="IW728_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1023984.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3200..4396
                     /locus_tag="IW728_000604"
     CDS             3200..4396
                     /locus_tag="IW728_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1023985.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4497..5873)
                     /locus_tag="IW728_000605"
     CDS             complement(4497..5873)
                     /locus_tag="IW728_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1023986.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5910..6776)
                     /locus_tag="IW728_000606"
     CDS             complement(5910..6776)
                     /locus_tag="IW728_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1023987.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6865..7890)
                     /locus_tag="IW728_000607"
     CDS             complement(6865..7890)
                     /locus_tag="IW728_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1023988.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7908..8582)
                     /locus_tag="IW728_000608"
     CDS             complement(7908..8582)
                     /locus_tag="IW728_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1023989.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8766..9659)
                     /locus_tag="IW728_000609"
     CDS             complement(8766..9659)
                     /locus_tag="IW728_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1023990.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9733..11430)
                     /locus_tag="IW728_000610"
     CDS             complement(9733..11430)
                     /locus_tag="IW728_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1023991.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11423..12736)
                     /locus_tag="IW728_000611"
     CDS             complement(11423..12736)
                     /locus_tag="IW728_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1023992.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12736..16773)
                     /locus_tag="IW728_000612"
     CDS             complement(12736..16773)
                     /locus_tag="IW728_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1023993.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16786..17244)
                     /locus_tag="IW728_000613"
     CDS             complement(16786..17244)
                     /locus_tag="IW728_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1023994.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17542..18060)
                     /locus_tag="IW728_000614"
     CDS             complement(17542..18060)
                     /locus_tag="IW728_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1023995.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18062..18679)
                     /locus_tag="IW728_000615"
     CDS             complement(18062..18679)
                     /locus_tag="IW728_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1023996.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18676..19539)
                     /locus_tag="IW728_000616"
     CDS             complement(18676..19539)
                     /locus_tag="IW728_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1023997.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(19526..20437)
                     /locus_tag="IW728_000617"
     CDS             complement(19526..20437)
                     /locus_tag="IW728_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1023998.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20478..21812)
                     /locus_tag="IW728_000618"
     CDS             complement(20478..21812)
                     /locus_tag="IW728_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1023999.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22020..22850
                     /locus_tag="IW728_000619"
     CDS             22020..22850
                     /locus_tag="IW728_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1024000.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23094..23343)
                     /locus_tag="IW728_000620"
                     /pseudo
     CDS             complement(23094..23343)
                     /locus_tag="IW728_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23534..23728)
                     /locus_tag="IW728_000621"
     CDS             complement(23534..23728)
                     /locus_tag="IW728_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024001.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         7179 a         5301 c         4219 g         7696 t
ORIGIN      
        1 tttatgctct tcttttatta ttttgtgctt tttattttat caaatcactt tgttttttta
       61 taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat gtccaacggg
      121 atcgaaactt gccacggaat ctttgaaaat atggagtttg tatcctagat tatacgcatc
      181 gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa tatctgtgat
      241 ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat aatgccgttt
      301 gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt ttaaagagcc
      361 gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc ggttctccgg
      421 atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat ccttttgatc
      481 aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg ctgtcccagt
      541 agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt
      601 catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc tgctgatcgt
      661 ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg
      721 ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg tccaacagcg
      781 gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc tttgatttta
      841 tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc gttcaaattc
      901 gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc ataatataga
      961 ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc atcaaaataa
     1021 gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat tccgccagct
     1081 gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact tgtggtaata
     1141 gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt acctaatgaa
     1201 cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag gttctctctc
     1261 aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt tttaattcct
     1321 tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat aacaataata
     1381 agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct gatccttgtt
     1441 ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat ctttttttct
     1501 tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct tgcttgtgct
     1561 tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat aagccgatcc
     1621 ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt ctttgcatca
     1681 caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg atacaactcc
     1741 agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc ttgtgcatga
     1801 taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt aagatggctc
     1861 agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt catttcggga
     1921 ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac tttttctaaa
     1981 ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg
     2041 acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg caaacgaact
     2101 tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact gattgttttt
     2161 tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat cataactgct
     2221 tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata gataaacctt
     2281 agattttctc taaatataga aaatccttct ttagtccgat actctttcta gtagtccgta
     2341 tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg ataaatgaca
     2401 tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg tcttgctatt
     2461 tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga taggtgtagt
     2521 tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc
     2581 tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat
     2641 ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa
     2701 tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag tcaaagcagc
     2761 gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag caagtcagtt
     2821 tctttatacg catctgccga atctagttga tttgacaaat aagtataatg agatcaatga
     2881 ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc agatcatcga
     2941 ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg atctagatga
     3001 tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata aatatgaacc
     3061 agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc agatgatgtc
     3121 tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg agaacagatt
     3181 caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg ttacaccagt
     3241 taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag atacgctgac
     3301 cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg ctcgtttaat
     3361 tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa aaatcgatgc
     3421 taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat taagtgaatt
     3481 ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag gagattcctt
     3541 aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg ccggagaagg
     3601 gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag tgacagcaaa
     3661 atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata aagaaaaaga
     3721 cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca aagactactt
     3781 tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac ttcaaacaac
     3841 gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg atgttcagat
     3901 tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg atcttcgctt
     3961 agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa acacaaatca
     4021 ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt ggaaaaacca
     4081 agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc aacgccaagt
     4141 ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa tctcagccat
     4201 cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc aaaccactca
     4261 aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag gaaaagaaaa
     4321 acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac aaaaactgtt
     4381 ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca acaacagttt
     4441 attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat tggagtctat
     4501 ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag tccaataacc
     4561 attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa
     4621 gtcaagatgg agacgattgg caatagcgga acgaatggca ctttgaattc tccagctttt
     4681 ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat caaataggcg
     4741 agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat
     4801 acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt ttgatcgatt
     4861 tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa acgagcaagt
     4921 gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac agcgatcacc
     4981 gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc attgactcct
     5041 agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta aaggacagta
     5101 gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt tttgacttcc
     5161 tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag agctgcacca
     5221 tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg tttaacgaaa
     5281 aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac catggcatta
     5341 ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa tatcactacg
     5401 agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc agttaatgct
     5461 tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga ccatccggaa
     5521 gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt cagccaggca
     5581 gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac tggcactcgc
     5641 gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc taagacaaat
     5701 gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac cacaaatatt
     5761 cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt tagttctttt
     5821 ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc cattgaagtt
     5881 cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc taaaaggata
     5941 ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt tttcgttttt
     6001 ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat ttgtgaaagt
     6061 gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat gtttcctaag
     6121 ccccataata atccacagat catgtttttc cacacaaact gatcgacttt aactttacgg
     6181 aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat aattgacatc
     6241 gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat cgtagagaag
     6301 atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc tatttttcct
     6361 tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa agcaataata
     6421 ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta
     6481 ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc catgtacttc
     6541 attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa aaatccgatc
     6601 aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa agaaaacaga
     6661 aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat tttaccactt
     6721 actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat ttccataacc
     6781 aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac aacacaagca
     6841 ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta agattgggaa
     6901 aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat ggatacctaa
     6961 tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg ttttgctaag
     7021 tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt ccgctgggat
     7081 accgatccct gaatctttaa ctgaaaggct gactccttgc gcttcctttt ctatgctcac
     7141 aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt ggttgaagat
     7201 aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg
     7261 aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa tgattccttt
     7321 tagtccatat tcttggatga ggtagtcccg cgagaaactg tcgagtcttg cataatacaa
     7381 aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg tatacttttg
     7441 atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa gtggcacttt
     7501 gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac tgattgctga
     7561 ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta ccagtttttc
     7621 ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc
     7681 taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa
     7741 gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga tgtttggcgc
     7801 cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca
     7861 ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca gggaacgata
     7921 tatccctgtc ctacttttgt ttcgatatat ccttggatac ctgcctgttc gatttttcgg
     7981 cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac
     8041 agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat caaaataaat
     8101 aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc catacttccg
     8161 ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa ttctgtgtag
     8221 ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat ttggaaatgt
     8281 tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat attcgtattc
     8341 ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg acaccaataa
     8401 tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc ttctctttca
     8461 aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg ccatttctca
     8521 agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat gatttttgcc
     8581 ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc cttgtgaaac
     8641 aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg
     8701 atttaatcgc gtttgtctct tccaagcagc ttaaatttat tatgttattc ttaataattg
     8761 gtctattatc tgttattttt ctttcgttgt ttttgtcttt taaattttcg ataacaccaa
     8821 taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa tcgataaaga
     8881 tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg aactcgtctt
     8941 cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact
     9001 agatcctgct tttctctaat tgctaatgta tttgtttttg ttggaaggac aacaattttc
     9061 tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg gtcatcttgc
     9121 tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg ggcggtaatc
     9181 acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac tgtcgctcct
     9241 ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt gattttcgga
     9301 atatagatgg ctccgatcat atgttgttcg taatattctt ttcctggatt ttggacattt
     9361 ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac tgatgtatga
     9421 gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt ttcttcttta
     9481 tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata attgctgaga
     9541 gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat cactgccata
     9601 aataattgta ataaaagact gatttgggtt tgaggtctat tacctatttt ctttttcatt
     9661 ttcttcacct tcttatttaa aaaaactgca gcaaccttta taaaaccgat tgctgcagct
     9721 tatttttgtt gcttatactt cagacttcat tttagattta cggtaccagc tgtacgctcc
     9781 aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac cagtagcagg
     9841 aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta ctcctacgat
     9901 ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg ctgcctcacc
     9961 tgttggaagt acataacctt ctggcgccat ggtctctttc atttgatagg ttccataatc
    10021 taacccttga acctcaaagt atccatttcc atctgcgaca aattctgttg cttgtccgct
    10081 tgttgcccac tcaattctat ctgctcctga ttcataaaca gctaaaggcg actccccttt
    10141 ataaaatatt gcgtattcag aacggtcttc attccacaat tcgaatcttg catctttaag
    10201 caattcactg cttgatgcat catatttttc aaactttctt ccacctgttg tgactttctc
    10261 tggcggctct actccttctt cataatcaaa ttcatcattt cgtccgattt caataaccgc
    10321 tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa accttcaaat tatacgtgat
    10381 cgttaaatct cctccacgtg catctgctaa tctacctaaa gtatctactt tggctactcc
    10441 attttcttct gtcaaaatcg tcacgacaaa cccgttgctt actacttcta ctatataatc
    10501 ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct atttcctgat cataataagt
    10561 tagaccaact gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc ttccgttttc
    10621 aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc
    10681 tctttctaga tcaatataag aaatcgtttc ttgttctcca gcttcattaa taatcgttac
    10741 ttgtctaagg tcgctttcct ctacattcat ctgtttatct gcttctctga tttcgttttt
    10801 cggatacaaa tgcacatctg tattgtatgt gtctttccat gtagaaccat cccacacgcc
    10861 atccaccata tcaggcatca tcactggcat cataacaacc attggagacg cgattctgga
    10921 aatatgagct ggcgaataag tttctaagaa aagatagact ttatctctag aagaaggtgc
    10981 ttcttccgtc acttggacgg tagagaatac cgcttctcca gcttcattcg ttgtttgttt
    11041 gtcaatactt gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt tttgaatcca
    11101 ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag gattatctcg
    11161 aatcaaatcg taatatactt ccgttacgtc aaacaaatca aaatcgactc ctggaatatt
    11221 ttcttcacca aaatccgtag aaaccaatcc gctattcggt cgttcttccg gaacagatga
    11281 gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga cttcttcggc
    11341 tttgactagt tttccgttcc cgaaaatccc tattcccgca gtaacgagta acagtacact
    11401 cattcccgtg acaatcattt ttttattcat ttttttctcc ccgttctttt tttattaata
    11461 aaacagcacc taacaataaa agaattccta gtaaagaaat caacgttctt gcttcattcg
    11521 tttgcgggaa tacggaccat tttcctgatt gacctggatt gcttggacca ctcggctctt
    11581 tcggtttgtc cgttgtttct tttacggtct cgttataaac acgtggtgtt gcggattcat
    11641 aggcagagac tggaactttc ccggattcta attcagccat ctcctgtccg ttaactacta
    11701 catattgtgg ttggcctttt gcatccaccc aaaattcagg gatgatgacc tcgattctct
    11761 tacttgcttc atccatctcg taaccttcca caccttttaa ttcttcaaaa taataggtgc
    11821 cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc tttatccgaa atgaatttgc
    11881 ttacattttt gtcattcaaa ggatcattgt ctgctggttt gatccattga tttttcaagt
    11941 cattagcagg agccatatct aaataatatt tccccccttc tatcatctga tatagagcaa
    12001 aaacagcacc ttctaatggt tttccttttt cagttgttcc tctttgacgt ccatacttga
    12061 agaagtatgg atctcttaga taaccgacat ttttaggata gatatgaatc tcttgcagtt
    12121 cctcggttga ttctgtcggg tgataaattg gcaaaatagc agcaattggg atagctgtct
    12181 gatcgatatc aacatccaat ccaacttctg gatcaatcga ttcttcgaaa atcaaataaa
    12241 ctgcatcccg atcttggttt ttcttaggtg ctttgatacg tgcaatcccg tctcctaatc
    12301 cgccaaccgt atctttccca gttgttaccg aagtccctat ttggacaagc ctttcttctg
    12361 tgattagctg acggatattg tttctgttcg tttcagcgat ttgctggacg aattctttag
    12421 gagtcatgtt ttcagcagct tttttttcat gataataatc ggtcatgtcg taaatggcaa
    12481 atgtcgctcc atttagtggc accgtctgtg aaatgatatc tgctgtttcc cctgcttcct
    12541 cagagatagc caagccatca ttttcatggt attcaaattg atcgttataa tcgatatctc
    12601 gaatcatccg tttatgcaac acgaattcca cgacatcttc ccctgattct tctgccgaca
    12661 tcgcatgtgg aagaaggact gctgcaagcg gtagtaaaac aagtaattga agcatcaaac
    12721 acaacagctt tttcattacc ttgctccctc ctttttagat tgtctgacaa acaaaccata
    12781 ggctgctcct gcaagcgcca tgaagaatgc agcaagcagg atatagaata atcgtccgga
    12841 tcctcctgta gcaggcaacg gattttcagg atcgttcccg acactaagct gtaatctgat
    12901 tcgatttgtc tccaaaagtt catattctaa ctcttgctgt agatcaggcg tctctgcttc
    12961 ttcgtttgga taatacgaaa tctcaacttt tagtgtaccc gcttcttttc ctggaactac
    13021 ccgatcattt tctatgtcag gttcttctcg ataactaatt tctaaaataa agtaacctgg
    13081 aagttttcta tagctgtcag gtgacttcac ttccttgatc cgatagaccc cttccaacaa
    13141 gtctgaagca ttgaataggg aagaatcgtt tggatctggt gccaagcctc cctcgatgat
    13201 ttcatactct cctgcactat tttgtttctc aattgaaaat tctgcgcctt caagacgtcg
    13261 gctattcata ctgtcaattt tcaaaagttc caaatcgatt ggcgctagtt catcttctaa
    13321 ggttacttgt agtacaccat cgatgatttc atagccttcc ggcaagggag cagtttcgtc
    13381 ttcgatgacc gtatcttcaa tgataaactt accttcttca ttcagtgcaa actggaacac
    13441 ttgatcagat agccgatacc catttggtgc gacagattcc gagacctcat attcaccaat
    13501 agacagatgc agaggttggt cgtaatttaa atcgacatac ttttctactc ctacggcacg
    13561 agaatagaag tatccgatcc caggcaaacg tttcgattct tctggttcta acggattacc
    13621 agtgtatgct cgatacagcg ggaaggaaga atcccccact ttcctgatat cgaaacctgc
    13681 accatctaat gtatggcctt catggtctaa ttttctaact cggatctccc gataatcaaa
    13741 cgtattgtaa gcagtgaatg ctacccataa acgatcctct tcatctgttg tatacgtgag
    13801 atattgccaa ttttcttcac ctgtttgctt gagttggata gtccctatag cattatcgta
    13861 aagtcgcaag atccatgtgt cattttcatc aggttctttg aaaccttctg ggttctggct
    13921 ctcaatgatt ttgtattctg catagttggt agtttcttca ctatccttat aaacaaatga
    13981 atctggctct ccgattggat gaataacttt ctcaccatcc acgattacat atttcccgtc
    14041 ttcatctact gaatagaacg tgagtctccc tttctcttcc tctacttcgt tcacaccaga
    14101 aagccctttc gctactggat atccgatttc cagtccgtta ccatctgttt cataaagttc
    14161 aaactctgcg ccattgattg gcatattact gccttcaatt agtttattga tattcatgtg
    14221 gattggcttg aattcatttc gcatttccag tttgacatac aactcatcat actctgtgcc
    14281 ttctatgtta ggctcttgta caacagaaag cttgtgatat tctccttcat aataggaatt
    14341 cggatcttta ccttccggtc cgccgactct cgaaccagat gtatattcat ctacaagctc
    14401 gtagtgacct aacatctcat cttcgttggc tgcatatttt ttgatttgga attgatacgt
    14461 ctcatctact aactgataac gtggtggtcc agcgatctct ctgatccaaa atatgtcgct
    14521 gactgctcca gtttcatctc ctaaaatcgg catcttcgtt tcaaagatcg ctattccgtc
    14581 cgtggtagtt ttacttgttt cgattggttc ggcttcccca ctctctggat tttggtcata
    14641 taatgcaaat tcgactccag ccaacggctc gccattttga cccttcttgc tgacttggaa
    14701 attacggcct ttatgttcgt aacgaatgga agaagatggg aaatacatcc gtccagtagt
    14761 gatatcattc tcataatcct ccaggttctg aatataggcc atattgttca ctgtgcaaaa
    14821 atttccgtct tgagctcccg catcaagttt tacttggtac ttgatcctga ccatttcccc
    14881 ttcacctaaa gaaatcggag aaaatttata agcttggttt tcccgtccga catacgttgt
    14941 cggagtcgcc actacttctg gtggggcttg tccatgacgt tcttcatccc atatttctgg
    15001 ctcttggtac ttcatgccgt tttctcctgg atctagatgg taaaactgaa tatctgccga
    15061 tctgccagct ccaaatggtg agacattaaa accagtttca tcataaagtg tggcttgctg
    15121 gatcgttctc ttgaaggtag aagagatatc ctccaaggct gtaacaatgt cattcttgtt
    15181 gttcgcattg taatatcgcg gtgttacgcc atcttcttct aaatcagcaa tatttttcaa
    15241 tacatttcgt ccaatagcat ctagtcgttg tctcccgcta acgtttccat cgattcctaa
    15301 accaatggag aagatacttg gcgggttctc ttgatacttg tttttcagcc aatgatgata
    15361 agcaactgtg ttgatcgcag atgacttttc ttgcgaagta ggactgccat tcgtattgtt
    15421 attagaagaa ccatcaccaa cccagtgagt cgctttatta tctccttctt ttacctctcc
    15481 ataacgatag tgtacattta cttcatcttc tggacggtta aagtcagatg cataggtaac
    15541 tggatgagta aaccctgcat tagaagtatt cccagaaaac tcctcaaaat ttctgaacaa
    15601 aacgtcatta tcttcagcaa tcttatcttc catcacagtc cagttgctcc aagctccatt
    15661 agtgctcgta cgataacttg actgaatcgg tgcataactg aaggtcggtg tcccatctcc
    15721 cacaacgatt aggatttttt ctggatttct attttctccg ccattgtctt tatacaatgt
    15781 ttcatagcca tttttaagcc ctagtgtaag cggagttcct ccgattggtg tacgatttaa
    15841 tgctgtattc tcacttcccg tcaaacgatt gatatcattt gtcaatggaa agatgtccga
    15901 actgatttgc tcatggttat tgatagctgt actattatgg taaaaattca ccataccgat
    15961 tcgaatatcc agactcgggt cagagagcag atctttactc atttcgttta ctgcctcaat
    16021 caacgcagca tttttagttt gactattggt cccagcggtt aattcactca tactcgctga
    16081 cttatctaga acaaaaacga tatcgattgg actgatttcc tttttctcgc ccccgatgac
    16141 atctagatag acattgtaaa gggttgttgc atcttcagga tgtagatcag atgctgtgct
    16201 gcttggcaga acaggttcta cccatttttt gatgtacgcc ccgttataat ccaagtaact
    16261 gttttcaaaa ttccgttcat ttccccaaag ctgaacggtt tgagctggaa cgtttctatc
    16321 ttcttcgcct gcagttgatc cattattagt agcatagtcg taatttcgcc agttcgctcc
    16381 cccgtttgta taatcactat catcgatcaa gttctctccg tcatatggcg agcctttgtc
    16441 tccttgatag cgttcattgc tttgtgtcca atttcctaac acttttgtat agtcattcgt
    16501 tgtaaacttc gggtagcgat gctgttcgtt ttcaacttct ccgctaggat agatatattc
    16561 ataggggtct tgcgtagctg ttggcccaag acgctccaaa ataccgttga ataactggcg
    16621 ttgaatgcgc tggagtgctt gaccgctggg tacgattggt tcgtccagct tttccatctc
    16681 attttcttcg ctacgttgaa tttcagcgga atcttctgta tcagtttcat tttcttgtaa
    16741 ttcttggtta tctattcttt catcaaccac cacttgaaga ggtagttacc cttcgttcac
    16801 atcggggatc ttttctgttt caaaaatcaa tgtgccggtt tctgcttctt ttgaataaac
    16861 ttttccgaca taccattctt gaccatcgat cgtctcgact tttaggtctt ctccatcttc
    16921 atcataggag gatagacctg ttccacgaac gttttttacg gtaccaatcc cggcggctgc
    16981 cgcatcgatt gctaaacgta ttctaccttc attggcactt tccttttttt caaaagccag
    17041 atgccattca agggtttctt ctttttcaat atacttccct tcaatttttg catagtcctc
    17101 atctaagaat acctcatcct cctttgtttc tgttgactca gcagctacat ccagggtcgt
    17161 catatgatag actgactgga aatatggtcc aaatagcaaa ctgacagtta ctaactgcaa
    17221 aagcttcaat tttaaccctt tcacatcctt atactccttt caaaaatatt tgtcttcttt
    17281 cacaacaatc aacaaaacaa tgcccatcta attataattt aatttaattt taattttaaa
    17341 tcaaatgata tcctttttag ttaaaaaaca ccaattatac aacatttatt attaaaatta
    17401 aattataaaa ttttataacg ttctcattta acaaaactta agttattaag acaaatgaat
    17461 aaacaactac gctttcctcg aaacaaaaaa ataaagcaca aatgcgcatt tacgcactca
    17521 tgctttattc gtacaattaa ttcagataac ggcagcgcat aggatgccaa taacacttcc
    17581 taaaatagct cctcctaaaa tgtcagtagg ataatgtaaa agtaaaaaaa tcctcgaata
    17641 agcgatcaaa caagcaaata aaaaggctaa tattcctgca actggactta attcgaacaa
    17701 cgcagctact gataaaaagg ctagcgctgt atgtcctgaa ggaaaagaag aagtcttcgg
    17761 tttacttgtg taaaggacaa gccgacgatt tttttcataa ggtcttttcc gtcttgtcgt
    17821 ttttttgata aaaagcaaac caacaaaaaa atagatcaaa ctgccgataa gaatggctag
    17881 accatattct ctagtatcag gtatcaagac taaaacagca gcaaggagca accaaataaa
    17941 accaccatta gagatattac tgataaattt caaaaaattg atttttcgac tatccgtttt
    18001 ccgttgcttg gcgaacttgt tgataatact atcatcgatt ttagcaatca aacgcaacat
    18061 gttattcctc ctttttaaga accttttctt gcgtacgatg gataaaaagc aggacttgaa
    18121 aataaaacaa gaaactgaaa cctaataaag caaaatagat tggtcctgca aaccagaatg
    18181 cgacaagaag gatcaacgtg cctgccaaat agaaaagcgc gcttttgaaa tcattaaaaa
    18241 acaagaaaaa tgcagtaatc caagaagctt ctttttgttc cagttgaagc aagattttgt
    18301 aactaaagag aagcgcgctt aatagaagca aaaacaacgt agtatacatc aatgtatagc
    18361 caattccccc accccaatag cggaaaatct gataatcgat caatagaaga agaaaataaa
    18421 cacttgctaa actatcttgc tttccataaa gagagaagtt ctcacgaaac tttttccaga
    18481 atagtgaacc gagctttttc tcttcatttt ttttgtaatg ataaatcata tacagaactg
    18541 ttcgacttgc agttcctaat gtgaaaacag gcaagacgca caggaaccat agtagctgta
    18601 aatggataag acgtggaatc cattcaatga tttctaagaa ataatcattg gcttttttac
    18661 caatcgatcg attcatcatc cttttgaacc tcctgtcaaa tccatccctt caatgaaata
    18721 acgttgtgca aaaaagaaca gcaaaatcgt cgggatcaag acaagtgttg ctcctgccat
    18781 cagataattc cattgtgtcg ttgcttggtt ctgaaaaact tgcaagccaa tctgtaacgt
    18841 gtatttttct tgatcttgga tatataacag aggacctaag aaatcattcc aagctgcgtt
    18901 gaaggaattg atcgcaatgg taatcaaggc aggtttcgtc aatggaagca tcaaatgact
    18961 ccaaatatac aaatgattcg ctccgtcgat ttcagcagct tcgatcaatt cattattgat
    19021 cgataagtaa aattgacgca tcagaaatat attgaacgca ttgccgaaga acgatggaac
    19081 gatcaggggc aaataagtac ctacccagcc gatcttggag aataatacat attgagggat
    19141 catcgttaca aaccccggga tcatcatcgt agaaagaacc aaagcaaaca tcagcttttt
    19201 cccaggaaaa tcaagcttcg caaatccata agcaataaaa gaatttgaaa ggacattacc
    19261 gatcactact agcacagtga tgaataatgt atttcttgca tagcgccaaa aaggaaaggc
    19321 ggcaatcgta tccagatagt tttcccatac gattttttct gggaaaaagg tcggaggata
    19381 acgaacgatt tcttccattg gtttcaatga agtaaacacc atccaaagca atggaatgat
    19441 caaaatgatt cctattactg tcatgaggac ataacttagt atttttttgt ttgccgattc
    19501 acactggtct ttttgaaata atccatcatt tgccgtcacc tccagaataa tagacccact
    19561 taggtgcaag cttcaaattg acaaacgtca gaaccaacac gatcagaaac aggatcattg
    19621 acatagccat cgcataaccc atatcaaaag ctacgaacgc cttgttccac atatgcagat
    19681 tataaaaaag caaagaattt gcaggtccgc ctgttccatt tttcgtcatg acataagctt
    19741 cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac atcgtaaaaa ataataggag
    19801 tgattaatgg caaagtaata cttttgaact ttccccaaac gcctgctcca tcgatttctg
    19861 ctgattcata taaagatcta ggaacatttt gtaatgtagc taagtaaagt agcattgctc
    19921 ctccagaact ccatattttc atcaaaatca acgcaggttt cgtccattgt ggatcaaaca
    19981 gccaagctgg tccagggata tggaaccagc ccagaaatgt gttgatcaat cctgcagatg
    20041 gagaaagcag ctgcatccat aagatgtaga ctgctactcc tgataagact gccggtaaat
    20101 agaaagctgt acggaataca ctgattcctt tgattttttg attgagtact gtggctaaaa
    20161 acacactgaa aatcgtcgtc agcggtacat tgaagatgac gaaataaacg gtgttgtata
    20221 acgatttcca aaataaatca tcgtgaacga acattctttt gaaatttgct agaccaatga
    20281 aattcatctt ggatgtcaaa ttataatcgg tgaaactacc aataactgaa aaaagcatag
    20341 gccctagcat aaataccaag aacccgataa taaaaggcga gataaataaa taggcccatc
    20401 ttgcttcttt tttctttcgg atagataaac gcttcaaatt ttcttgcctc ctctgtacct
    20461 agacagtcaa aaatggatta ttgattttgc ttgactaagt tttctactgc tttttgtgca
    20521 tctgccaatc cttcttttgg tgattttgtt cctaaaagaa tctggtcaat ctgttcattc
    20581 actaatgtcg tgtaatccgg agcatctaaa ggaactggtg tgatcaccgt atttttaaag
    20641 ttttcgtccg ccatttgata gatcattttt cctttatccg ttaggttgtc attattcact
    20701 aaattctcat tggctgtctt gctcgccatg atatcaaaac ttttttcgcc gaatttttct
    20761 tgtacttctg gagaagaaag atatttgatg aattcataag aggcctcagg atcttttgct
    20821 ccgtatggta cttccaagac gaatccgcca ccccacgacc aatggcctga accttcttct
    20881 ttttctggga gctgcatcac gccaaaatca aaatcatccg gtgcattttc catcaaacta
    20941 gagtaataat tgatattttg ggcacgcatg gctactaatc cggaaataaa tggatcagat
    21001 acaccagacc caaattcggc ttctaattta ttgatcgtat ttttcccata ataatcttgc
    21061 cagtcttgga tccactccaa ggcttccact ttatttttag tatcaatctt gactttttca
    21121 tctttgtcaa accaactagt cccgttatct gcgtttagcg cccatacatc agcccctaaa
    21181 ttccataatg gataaaaacc gatgcgtgta aattcttttc cttctttttt atctagttta
    21241 cgtgctgttt cttctaattc atcccaagtt gtcggtagat cttgttcact gattccagct
    21301 tctttaaata tctttttatt ataaaaaatg acttgtgtat ctgtattgaa tgggatcgca
    21361 taaggatcac cttcatattc tactgtatcc caaagctgcg gataaaaatc cttagaagtg
    21421 tctttttcta catatttgga gagattagtt gcttgctgtg cttctgctct ttgggcaacg
    21481 gaatttatgt cctgtacaat aacatcgggt gcatttccag ctgttattgc agataaagat
    21541 ttagtccaga tgtcacccca tggctgatac ttataattta catggatctc gttttgtgat
    21601 tgattgaaat cctcaatgat ttcttcgata gcttcttttc gtgcaccgga tccccaaaag
    21661 gaccagaaat caatattttt tcttccatca cttgtttttt cagaagaatt tgctgcactt
    21721 gaactggcat cgcttgcatt acttgatccg catcctccta ataaaaacaa tgctgaactt
    21781 gccagcaacc ctgtaaacaa taattttttc atattttacc tccaaatttt aatgtaaccg
    21841 atgccggttt taatataacg atatcatttt gttctgaaaa taacaattta taaggtatga
    21901 ttctaaaaaa attattaatg ataccaatac tttatataat tcgcgagcgc tttctttgaa
    21961 ttttttaatc tttcgtagca gaatgagaat gtattaaaaa tagattagga ggaattttga
    22021 tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc agatcgtccg aatccattaa
    22081 tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg cgagatgagt gtagctatgc
    22141 agtacctgtt ccaaggctgg agctgccgag gagaagaaaa atataaagat atgctgatgg
    22201 acattgcaac agaagaaatc ggccacgtgg aaatgttggc gacaatggtt gctcgtttat
    22261 tagagggtgc gcctgttgaa gatcaagaag cagcgatgca aaaagatcca acaatcggtg
    22321 ccattatctc tggaatgaat ccacagcacg cgatcgtagc tggcttagga cctcgtcctg
    22381 ctgacagcgt gggcaatcca tggagcggtg cctatatcat tgcgagtggc aacttgttag
    22441 cagactttag agctaattta aatgccgaat cacaaggaag acttcaagta gcacgtattt
    22501 acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc tgttctcctt gcaagagata
    22561 gctatcatca aaatctatgg gaatgtgcag tgaaagaatt agaagaaaaa gaaggttcaa
    22621 tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa agacattgct tatgacttct
    22681 ataacttctc agagggagac caaagtagtg aaggttcttg ggctagtggc aaagcactgg
    22741 atggcgaagg cgaatatcaa tggttgaaag aacctaagat cgaaggcgct gcaccaaaat
    22801 tagaaccagt aacaccaaaa atgtatggga cacctccatt caagaaataa atatctaccc
    22861 gtagaaacac ccgttttttg ttccacacgc tttgtgagca aaactgacta aagtcattaa
    22921 tcataaccac ccttcaaaag ggtggttttc tctagagcta taagctcaaa tcaccggcca
    22981 gcgcctaaag acgctggctt tcatatttta tgttcaagcc atattgcctt tgccagtttt
    23041 gcccacctct taaaaggttt atgtactact ccttagccct taaaaggatc gtcttactct
    23101 cgtacactca gcttatctaa cgcactatca tgtttttctt gttcttgaat gtactttttg
    23161 atggtcgctt cattttgtcc tacttttgtc acatagtatc cttccaccca aaagtgtcga
    23221 tacccaaatt tatattttag atttgcacaa tctcttgatg atctcctgaa tattggctcg
    23281 atattgatta taaattattt ttcttctata ctttggtgtg aatacaatgt ggtatttaca
    23341 catctacttt gtacgggcta aactatttcc ctttattgct ccacccgcat agcaagtggt
    23401 tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa gacaataaat ctaaaaaccc
    23461 gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa tgaaatgaaa aagatctctt
    23521 acggcaatct attttatttt tttgattttt tataaatgga aaatgcttca agaagtccaa
    23581 gaaataaagc cgtataaaaa caagaaccta tgaaatcttt atttattaga tattcaacag
    23641 aaatcccgat aacactagcc aacaatatat ttatcagaag tgtaaaataa ggatgctttt
    23701 tatagaatag aactagtgtt gttttcatag actctccttt cttcatattt ccaattaatc
    23761 acaaattcac ggcattacta gaaaaattaa aggggcgcag aagtggaaaa atactcttcg
    23821 actttcccta caaaccaggc catcttgtgt ttaataacta taccaaatct aaggtacctc
    23881 taaatctaaa ttccatttgt ggcggtgacc tccaattttt aactcttcta tctcccatcc
    23941 agtatacata accttgatac ttcgaccact tatatatcga acacacaaat acctatccat
    24001 tgcaggagta ctacctaact tagttgctac ttgcatctaa ataacagctg aaaagatagg
    24061 acttactctt agtaaagtat ctgaactatt aatttttaaa ctatcatttt tatcctcaac
    24121 tacatgcaca tctgttgcaa cttcactttc tgtctcatcc agataaatga ttaaggaatc
    24181 gggaattgca atatcttcag ctgacgctgg aatagttgag ctaaattagt catcatatct
    24241 ttattataaa ataaccaaca gcaaaaaata caatgaaaaa caaacttgga actaatgtat
    24301 cattaataaa tgttgaaaaa ttcagcaact cattactgta tgattttagc agcccaacca
    24361 acatataccc tataaaagct aataagatgg ttagg
//
LOCUS       DACEMO010000020        24344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEMO010000020 DACEMO010000000
VERSION     DACEMO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.020"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..846)
                     /locus_tag="IW728_000622"
     CDS             complement(<1..846)
                     /locus_tag="IW728_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1024002.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1070..3010)
                     /gene="thrS"
                     /locus_tag="IW728_000623"
     CDS             complement(1070..3010)
                     /gene="thrS"
                     /locus_tag="IW728_000623"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1024003.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3347..3526)
                     /locus_tag="IW728_000624"
     CDS             complement(3347..3526)
                     /locus_tag="IW728_000624"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1024004.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3573..5396)
                     /gene="cadA"
                     /locus_tag="IW728_000625"
     CDS             complement(3573..5396)
                     /gene="cadA"
                     /locus_tag="IW728_000625"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1024005.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5674..7953
                     /locus_tag="IW728_000626"
     CDS             5674..7953
                     /locus_tag="IW728_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1024006.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPTHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8046..8693)
                     /locus_tag="IW728_000627"
     CDS             complement(8046..8693)
                     /locus_tag="IW728_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024007.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8872..9591)
                     /locus_tag="IW728_000628"
     CDS             complement(8872..9591)
                     /locus_tag="IW728_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1024008.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9722..10873)
                     /locus_tag="IW728_000629"
     CDS             complement(9722..10873)
                     /locus_tag="IW728_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1024009.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11090..11830)
                     /locus_tag="IW728_000630"
     CDS             complement(11090..11830)
                     /locus_tag="IW728_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1024010.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11834..12157)
                     /gene="rsfS"
                     /locus_tag="IW728_000631"
     CDS             complement(11834..12157)
                     /gene="rsfS"
                     /locus_tag="IW728_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1024011.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12214..12807)
                     /locus_tag="IW728_000632"
     CDS             complement(12214..12807)
                     /locus_tag="IW728_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1024012.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12791..13438)
                     /locus_tag="IW728_000633"
     CDS             complement(12791..13438)
                     /locus_tag="IW728_000633"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1024013.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13451..13762)
                     /gene="yhbY"
                     /locus_tag="IW728_000634"
     CDS             complement(13451..13762)
                     /gene="yhbY"
                     /locus_tag="IW728_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1024014.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13778..14887)
                     /gene="yqeH"
                     /locus_tag="IW728_000635"
     CDS             complement(13778..14887)
                     /gene="yqeH"
                     /locus_tag="IW728_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1024015.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14884..15411)
                     /locus_tag="IW728_000636"
     CDS             complement(14884..15411)
                     /locus_tag="IW728_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1024016.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15591..16376)
                     /locus_tag="IW728_000637"
     CDS             complement(15591..16376)
                     /locus_tag="IW728_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1024017.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16369..17238)
                     /locus_tag="IW728_000638"
     CDS             complement(16369..17238)
                     /locus_tag="IW728_000638"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1024018.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17239..18618)
                     /locus_tag="IW728_000639"
     CDS             complement(17239..18618)
                     /locus_tag="IW728_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1024019.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18626..19045)
                     /gene="fabZ"
                     /locus_tag="IW728_000640"
     CDS             complement(18626..19045)
                     /gene="fabZ"
                     /locus_tag="IW728_000640"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1024020.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKTSAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19070..19546)
                     /gene="accB"
                     /locus_tag="IW728_000641"
     CDS             complement(19070..19546)
                     /gene="accB"
                     /locus_tag="IW728_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1024021.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19549..20784)
                     /gene="fabF"
                     /locus_tag="IW728_000642"
     CDS             complement(19549..20784)
                     /gene="fabF"
                     /locus_tag="IW728_000642"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1024022.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20803..21540)
                     /gene="fabG"
                     /locus_tag="IW728_000643"
     CDS             complement(20803..21540)
                     /gene="fabG"
                     /locus_tag="IW728_000643"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1024023.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21552..22469)
                     /gene="fabD"
                     /locus_tag="IW728_000644"
     CDS             complement(21552..22469)
                     /gene="fabD"
                     /locus_tag="IW728_000644"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1024024.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22576..22800)
                     /locus_tag="IW728_000645"
     CDS             complement(22576..22800)
                     /locus_tag="IW728_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1024025.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22853..23815)
                     /locus_tag="IW728_000646"
     CDS             complement(22853..23815)
                     /locus_tag="IW728_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1024026.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23825..24265)
                     /locus_tag="IW728_000647"
     CDS             complement(23825..24265)
                     /locus_tag="IW728_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1024027.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6653 a         5428 c         4243 g         8020 t
ORIGIN      
        1 tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt tttttcactt tttcgctaag
       61 cttttcgttt ttagccatat agaaataaaa aatgatatcg atcgtagcaa attgggccaa
      121 aagtgaatta gttgccgcac ttcgattcgt gatttctttt ggtctagatg tttgtacgag
      181 tacatcggct atcttgctaa gcgggttatt tccaaattgc gtcaacgcaa tgatttcgat
      241 attcatgctt ttcgccagct cggctaatgc gactacatct gcacttctac ctgagtttga
      301 aacaagccaa agcactttct ttttctcatt gctgacaagt tgcgggagca agacgtggat
      361 atctttttca aaaatgattg gcttaccgac acgagaccat ttttgcagaa tatcttctac
      421 tactaaagaa gaggcgccaa ccccatataa aaaaacagtc tctgccgatt ccaaacactc
      481 tacgactttt tggatcgttt cttcttctag aatatctact gtttctttta tcgctacttg
      541 tgcattgtta ccaagtttgt ttttgataga gtaaaaggac tcatttgctt ctatatcact
      601 aaaaccttca tcgattccag tctgtagttc tgttgaaatc gtgattttga agtccgtcag
      661 actttgaaaa ccgatttttt tggaaaaacg tacaactgtc ggcgcactca acccggaagc
      721 ttctgctaat tcattagctg tcattgtagg gaccttctca atattaccga taatgtagtt
      781 ggccaacttt ttttctgctg aagaataatc ttccatcata ctttcaatac gactaattac
      841 gctcataagt tcctccaccc ttttaatgat agtctatgta agtataccat atcagaccct
      901 tattcacggt cgtatactgg ataaaaaaca aaaaaacaat ccgcccaaca gaatatctta
      961 tcccatcaat cccacagatt ctattgagca tctgtgtctt tgaggaatac ctgttatcta
     1021 ttgttcggat tgctttctat ctactacttt tttacaggaa atttacctat tagttttctc
     1081 tgctgtaatt tttgacttct gcttccatag catcaataaa gatattcaaa tcttctacag
     1141 ctgtttcttt acttccataa cgacgtacgt tcactgttgc atcttccaat tccttgtctc
     1201 caacaactaa ttggtaaggg atcttttgtg tttgagaggc acgtatctta tagcccattt
     1261 tttcattacg atcatcaact tctacacgca accccttcat ttgcaaacgt tctttgattt
     1321 cataagcata atcgccatgt gcatcaacag aaacagggat gattgttgct tgtataggtg
     1381 ccaaccatgt tggaaaagcg cctttgtata cttctgttaa ataagcgaca aaacgttcca
     1441 ttgtagaaac aattccgcgg tggataacta ctggacgatg cgtattttcc ccgtcttctc
     1501 caacatatgt caaatcaaag cgctctggca ataagaagtc taattgtata gttgataatg
     1561 tttcttctgt accaagagct gttttgactt gaacatctag ttttggtccg taaaaggctg
     1621 cctcgccttc tgcttcaaag taatctagtt ctaattcatc catagcggct ttcagcattg
     1681 cttgggcttt ttcccacatt gcgtcatcat caaaatattt atctgtattg tttggatcgc
     1741 gatagctcaa gcggaaacga taatctgtaa tattgaagtc agcatatact gccaccatca
     1801 attcaagcgt acgtttaaat tcatctttga tttgatcagg acgaacaaat gtatgaccat
     1861 cattcaacgt catttctcgt acacgttgta aacctgacaa agcacctgat ttttcatagc
     1921 gatgcatcat tcctaattca gcgatacgaa ttggtaattc acgataagaa tggatcgtgt
     1981 ttttgtagac catcatgtga tgagggcagt tcattgggcg aagaacaagc atttcgccat
     2041 ctcccatatc cattggcggg aacatgtctt cttgataatg atcccagtgt ccagatgttt
     2101 tgtacaactc aacatctccc attattggtg tatatacatg ttggtaacct agactaactt
     2161 ctttgtctac gatataacgt tcgatcgtgc gtcggattgt tgcccctttt ggaagccaga
     2221 atggcaaacc agaacctact tctggggaaa ccatgaacaa gtcaagttct tttcctagtt
     2281 tacgatggtc tctttctttt gcttcttcac ggagacggat atattctttc aaagctttct
     2341 tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg attgtttgag tttccacgcc
     2401 aataagcacc tgccactgat aataatttaa atacttgtat acggccagtg gatggaacat
     2461 gtggtccacg acacaaatcg atgaattcgc cttgttgata agcagtaatc acttcttctt
     2521 ctggcaattc actaatcagt tctactttgt acggatcact tgcaaaaatt tctaatgctt
     2581 cctgttttga aagaacacgg cgttcgatcg gcagattttc tttgacgatt ttcatcattt
     2641 ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt ttgtccatta tctgtatcgt
     2701 agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg gatatttgga tagaggcgac
     2761 gcatggcttg ggccatcaaa tgagctgctg aatgacggac taagccaagt gcatcttcat
     2821 ggtctggtgt gatgatctca atacttgcat cttcctcgat tggacgagtc aaatcaacga
     2881 gttttccgtt cactttacca gctaaagctt tttttgctaa gctttttgaa atgctttcag
     2941 caattgccaa tgttgttgtt ccagactcaa attctttgac tgcaccatct ggaaaagtaa
     3001 tttttatcat aatagtttcc tccttgaaat gttttgattg atcagatcat caagctgaaa
     3061 aacaaaaaaa tcctagcatc tcttgaaaag agatactagg acgaaaagtt cgtggttcca
     3121 cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa cggtccgacc gcccttgctt
     3181 caacaagggt ttcgaaagtg gtcatccttt ggttttctcc tggaaaagtt tcagcctagc
     3241 ttttcctctc ttatcgaaga ctctcaaatt cagttggctt tctcatcaat caattatgtg
     3301 tttatttaac cactaataaa tagaaaaatc aagtgggaaa ctaaacttat tttttccatt
     3361 ccccatttac accgtatgtt ccttttttca tctcattgat gatgacatgg atgtgttctt
     3421 ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc tttcatcata ttttctaatt
     3481 gttccggact gcgaccttct actaattcga catgtacgaa tggcataatg ctctctcctc
     3541 tttctgatcg ttcttttttc aactcgattc tatcatacta tgtttgtctt gggtactgaa
     3601 tcaggaaaaa tggctggatc tttttttgct ctcaaggcac tcaaaataga gaccgtatcc
     3661 acgacttctt gcaaaacagc tccgaataat gcggggatta gtcccgtact tgcaattaac
     3721 atcaaaaaaa tacaaataaa gatgccaatc agtacagact gtttcgctat tctcatcgta
     3781 tctcttgaaa tcactactgc ttttgctaca cgcaagagat catctttcaa gatgaccgca
     3841 tctgcacttt cactagcagc tgtagaacca tgagccccca ttgcaatacc gatatctgca
     3901 gcagcaagtg ctggtgcgtc attcacgccg tctcctacca tcgcaacagg tctttgatct
     3961 ttagggatac tttctaaaat ggtcagttta tcttgaggca agctacgggc atgtacagaa
     4021 tctaattgga ctttttctgc aatcgtttga gcaatggcag atccatcccc ggtgatcatc
     4081 atcaaatttt tcacatcaag ttttcttagc tccttgattg tctttgctgc ttctgggcga
     4141 atctgatccg aaaataggat cgttcctgca aaagatcgat caattgttac atagattgct
     4201 gtattggacg tttctatttc ttcagctgaa acaaaagagg cacgtccaat ctttacttca
     4261 tgattattga ctactgcttg gattccttga ccggtcactt ctttcagttg actaacagga
     4321 tacagttggc tgtttttcac gttgttcact aacgaacgag ctaagatatg gctagattcc
     4381 tgttctgcac tagctgcata cagaagtagc tcttcttttt ttattccatt gactggctga
     4441 atcgcctcta cttccaatat ccctttggta agcgttcctg ttttatcaaa agcaatcgtt
     4501 tttgtacgtg cgagcttttc aatcgttgtt ccagttttta caacgatccc gtttttactt
     4561 gaacggctca ttcctgcaac taaagcaatg ggcgcagcta gaatcagtgg acaaggtgat
     4621 gccactacca gtacctcagc aaatcgtaaa gggtcttttg agaaccacca agaaatacca
     4681 ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac gatcagctaa acggacaaat
     4741 cgggcaggct tttcttttga ttcttcgact agtttaataa tcatttgata ctggctgtct
     4801 gcggcacgtt tttcagcgat catttggatc gatccgtctc catttaaaga ccctgacata
     4861 atctgatctc caggtttctt ggtttccggg cgggactcac ctgtcaaaga agattcatcg
     4921 accgaagatt ctcctttggt cactcgacca tctgcgggta caagttcgcc aggcttgact
     4981 agtaactggt catcaatatt tatttcatcg attactatat cctgcaattt tccttctttg
     5041 aatcgatggg caattcttgg ggtgttatct aacaaagact gcagttcttt acccgctcgt
     5101 ttattcgcgt aatcctctag actatctccg cccgtaagca tgatcaatat catcaaacta
     5161 gcccagtatt ctttgacggc taatgttgca aggattgcgg taatcgctaa tacatctacg
     5221 ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa acatcaagaa ggcgagaatc
     5281 cctccagtaa atgcgacaat cgcaaatgca agggccggct ggtgaaaaac aaattcactt
     5341 aataaagcta tcccaccaat aagcaaagta agtaaaaatt ttttgaaatg tgtcattgtt
     5401 ttttctcctc ttttatgtga cttagagagg ttagtaataa atttccagct gcctctttca
     5461 tcttaagcat agtccttgac tagcactttt taaaacaata ggccttaatt gaaaacaact
     5521 atcactatca gcgtttccaa aattcatcag ttctacaaac aaaaatattc agcaaggatt
     5581 actttttcga ttcatcagtt aaaaagtacc ttttttcgaa aaactatgct atacttttaa
     5641 ttcgttattt acggcggaag gagcaaacat gcgatgaatg agaaacaact agaacaaacg
     5701 cacgtggatg aaacgatcga actaatccag atcgaacaaa atatgctgga tacaaaacaa
     5761 caagaattga ctcaggaaat gaatcattcg acaaaagaca cagctaatca caagattcga
     5821 gctggttcca atgaagcttt ttatgaatct gctgttgaat atcgccaaca tgagcaggaa
     5881 ttgcttttga aataccatac tcttgaagca cagcaaaaac gcctgcaaac gttgtctgtc
     5941 atgaaagata gtccttattt tgcccgtatc gactttaaag aagaccgtga aacagaaaca
     6001 ttatatctag gtatcgcttc tttaagagat ctggcagaag aaacaatcgt tattgactgg
     6061 cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg gtcccgcatt ttatgaaact
     6121 gaaacagata gaatagatgt cgatctgcag ttgaaacgac aatttaaaat ccaagaagga
     6181 cgtattttat ctatggtaga tacgtcggaa gtaatcaatg atgacttttt gctagagata
     6241 ttggatgaat catcaagcaa ccatatgaaa aacatcgtct ctacgataca aaaagcacaa
     6301 aatgcaatca ttcgggacac aacaagtaaa gtaatgctga ttgaaggaat cgccggaagt
     6361 ggaaagacat ccgctttgct tcaacgtatt gcttttctgt tgtatcataa tcgcaagtgg
     6421 ctcgaggctg aaaacgttct tctattttca cccacccatt tgttttctga ttacatctcg
     6481 actgtattgc cttcattagg tgaaagcggc gtgcctacac agacattcaa aaattatttg
     6541 gtgcagctgc tgccagagtt ttctttacta gatgaacagc aacaagaaag tggttttttg
     6601 ctaggggaaa aagatccgat acaagtgatg aaaagcggat tgaccttagt cgatcagatt
     6661 gatcgctata tccaaggcat cacaaactac ggtcccttgt tccgtgatat gaaaataaat
     6721 ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt ataaagaaac aaatgaacaa
     6781 ttacctttac atcatagact ttctctatta caaacgaagc tcctgaaaaa attaggcggt
     6841 ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc tggcggaaga acagctccaa
     6901 gaactatacg caacagatcc aaatcttgaa tatacagaac aaaaggagcg ttcattacgt
     6961 aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc gaatccatcg agggatcatg
     7021 aattatcaat ttgtcaatat aacgaaacaa tatctccact ttctacaaac tgtgccactt
     7081 tctcttctaa cacgacataa aatcagcgag accaggtggc aggaacattt gacaactgtt
     7141 cgtacacact tgcgtcaaag agaattgcgt caagaggatg ctgtactatt ctttttactg
     7201 atgcgccgaa catatccagt gacggttaca cacaaagctc gctatatctt tattgatgaa
     7261 atgcaggatt tcgcaccatc acaagtggca ttgctgcaag ccttgttccc aaaagcaaat
     7321 ttgactttct gtggagatct aaatcaaaat gtctttggaa atgaaacgat cgttggatca
     7381 ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc aattaacgac aagctatcgc
     7441 tccacaaaag aaatcactga ttttgctaat cattttattg tgaaagaaca tcaagtagaa
     7501 acaacagcaa gaaaaggaga ccttcctttg ctcatccaag gaacaacaga tgatatcaaa
     7561 ttgaattggt tagcagatac cattcgaacg actcagggaa aagcccggta ttggcgtaca
     7621 gcaatcatag ctaaaacaca agaagaatgc gaagctcttt atgctcaact gccagacgag
     7681 ttgaaacaac aagtccagct gattttaaat gagagtgact ttatgaagcg ttccattatt
     7741 ttacttccag cctttttagc aaaaggtctg gagtttgacc gagttttctt gtgggatgta
     7801 gatgcagaac atttttcttc tcagcaagac aaattgatcc tttacaccat gtgtacacgt
     7861 gcgatgcatg aattgaccct ttttacctcc aataatgaaa gtcctttgtt aaacaatatg
     7921 gatatttcat cttatgagaa aaaagtactg taagtaaaaa gacaactgct atcattaatg
     7981 ttagcagttg tctttttatt tacaataata attgcagaaa aagtaattca atcatttaag
     8041 gtcatttatt ctatatataa atccaaatct gtttcaggat attttaattt gacaagcgaa
     8101 ataatcattt tcagttcttc catgttttct ggatattctg ggtcaaatac cttgctatga
     8161 cggccaatcc cgccattgtc gatcaatcgg taaattcgat tccattcaat gaattggtca
     8221 attgattgga aattgctgat tggatagccg ccacaatcca aaatctctga tacacgaaat
     8281 gctaggtatg cactatcgat cactttattt tttttcgtgt ctatcttctc atttctttcg
     8341 ataagaattt tttcaggacg atgtttcaag ctccactcta atagtagaaa gaaagcctcc
     8401 atccgatact cttcttcaaa taaccgaaag gctgaatcag aacgacgaaa aagcagcgga
     8461 atcagataat tttgtctata aatcagcatt ggtgcgcggt ccaccataca ctctaaataa
     8521 cggttcagat accctgtacc gacatagttt cctaatcgat agccttcaac gccgaatctt
     8581 gcgtattttt gcgctaattt tgaacaatca gttacgttca cagctaactc ttctactttg
     8641 cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga tttttgcttc catattcttc
     8701 cccgtccatc ttccacatac ttcatatcat ttcacatgat acttatgctc tcatcatacc
     8761 acaggttgaa ttagaaaaga aaataaatca cacaaaaaag caagacaatc tgttagtgag
     8821 attgtcttgc ttttcgaaaa agactgaata cttttcgtta aaactggttg cttagtcttc
     8881 taatgccaca ttatggaaca catgctgtac atcttctaga tcttccagtg catcaatcat
     8941 tttttcaaat gtttcttgat cttctggaga tagtgttacc tcgttttgag ggatcatttg
     9001 gatctcagcg attgaaaaat cagtgattcc tttttctttt aatgcttctt gtatactatg
     9061 gaaatccgct acttcaccgt aaacgatgat ttgtccttct tcttccatta catcgcgaac
     9121 atcgatgtct ttttccatta ggtattcaag gatctcgtca gcgtcttctc ctgcaaaagc
     9181 aaagattgct gtattgtcaa acatgtaaga aaccgctccg gaaacaccca tgttcccgcc
     9241 atttttccca aatgctgccc gtacgtctgc tgctgttcga ttcacattgt tcgtcaatgt
     9301 atcaacaatc accattgagc cgcttggtcc aaatccttca tagcgcaatt cagagtatgt
     9361 ttcatctcct gcaccttttg ctttttcgat tgctcggtca ataatatgtt ttggtacatt
     9421 ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg tttgcatgcg gatcaggatc
     9481 acctgatttt gctgccgcgt agatttcaat cccaaatttt gcataaatcc ggctattatt
     9541 agcatcttta gcagttttct ttgctacgat atttgcccat ttacgtccca tattttcact
     9601 tcgctttctg atttgatagt ttcataacgc aaaaacgtcc tgttccatta tacttgtgaa
     9661 tcacgattct gtgaagcttt ttttctttct tccttcattc ttctcatttt tagtcaatta
     9721 tctatactcg gattgggatt tgcccgaaat tctgttccgg aatattgcct cccaattgat
     9781 agatttgatc acttctaact aacagttccg cttcttgtgc ctcttttttt ccgattcggg
     9841 aataaataga aatctctttt ttttctttta agaatcttct tccagcatcg gtaaatccaa
     9901 gtagtcgaag ataattttta ttttgttgga caggaatctc ttgctttttg atattcagca
     9961 agacataaca agataatcgt tggatccgtg tccatgtata tctttttgtt tttacctttg
    10021 acacaaaatc ctcaaaacta tcagcagttg cagctgctgt tttcatgcgc gattctattc
    10081 cttcggtcat ctgatagata tctgtcagtt ccagcaaagt agaagataac aaccgataac
    10141 gcaaatacgg ccaataagat tcccaagtct gcagcgaaca agttgaaaga tcttcttctg
    10201 tctgaggcgg cacaagtcca gctattcttt ttccttcttc taacgcatgg cgaatggctg
    10261 ttgcactggc tatcctctga tctgtgatga ttttttcatg atagcctgca cttttacgtc
    10321 ggatcggatg taattccata gggtggacat acttggcatt ttcctttgca taacttaatc
    10381 ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc gggatacaga ctgcgaaaaa
    10441 cctcagtcat tttttgcgga taactatacg tttgatcaga cagttcttga aatgcctctt
    10501 ctatcctatc ttgattctct tggacaaagc gtccgaaact gctgtaatca aacgaagtct
    10561 cttcttctgt tccaaaacac aaactgtcac agtccagtgt ttgcaagatt tttactgcac
    10621 ctttagcaaa atagtcagct gactgaaccg cccattcgac gggaagctcc actactaaat
    10681 ccacaccatt ttgtaaagct tcattcgcgc gatgccattt atcgatgata gccggttcgc
    10741 ctctttgcaa aaaattcccg ctcattacag caatgacgac ctctgctcct gttatttttc
    10801 gtgccatttc cacatgatag cgatgtccat tgtgaaaagg attgtactct acgataatcc
    10861 cgcagctttt catgcgcatc ctccttcata gttatcgata aaaaagaaat agagacaagt
    10921 aaaaaaaggc aaaaatcgga aaaaataccc gaactatata aactattctc gcaaacacaa
    10981 tacttctgcg acgaaaagct gcactgcaag agcaatgcct atatcgttcg gatatataat
    11041 ttatgttgaa gatttattct tttcttgtta actcgagcag gcaaaaccgc tagtctttgt
    11101 atgctacaaa gaaccagcgc tttgatttct ctgatgctgc ttcgtctgta aaatcagaaa
    11161 atgcttcgac tttcgaaaag ccgctgtttt ccagcattct taaataactt tccaatggat
    11221 aggtacgctc ttgatgtagt tcatcttcac gaataaatcg accagaccct tcttgttctt
    11281 caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc gcttcctgga taactatccc
    11341 acaaaaaagc aaaattatcc ccttgataat gatagctata ttctggaaaa acctcatcta
    11401 cttgataggt ggagtgtacg tcaaataaaa atcggccatt ttcttctaac gcttgataga
    11461 cttcatcaaa cacttgctgt acctcttgtc tatttttcat atagcacagc gaatcagaaa
    11521 aacaggtaat tgcctgatat tgtccgattt ctgataaatc gagcatattg ccttccacaa
    11581 attggatcga tgcgtcttct tcatatgctc gctggctggc aatcatcaac atctcttctg
    11641 aaaggtctaa ccctgtcact ttatatccat cttttgcaaa ggcaaccgct aatgctccag
    11701 taccgcatgc cagttccaaa atattttttg tgtcttcttg taaatgacgt ttcgaaaaag
    11761 ctaaccattg ctgataaagt gtttcgtcca tcacttcatc gtaaatgaaa gcaaatgttt
    11821 cataagccat atgttaatcc aaccattcac taagatcgac tagcggagca tctgaccata
    11881 gtttttccag attatagaaa tttcgttctg gcgcatggaa aacatgtacg atcacatctc
    11941 caagatcgat caatacccat tttccgcctt cttttccttc aatacgtttt acttcatatt
    12001 tgttttcttc ttctttatca atgatttctt ctgtaattgc gttgatttga cgttcgctat
    12061 ttgctgaaca gatcatgaaa taatctgcca acaaggaaac atttcgaacg tctaaagcga
    12121 taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc aatttttaat tgttctttac
    12181 tatctatgat gatttcctcc taatatgagc agcttatttt tttgctaccc agtaattata
    12241 tgtttcgatt gtttttggat aaatctgttg ttcttgcgca agcaaatggg ccaatgtatg
    12301 ttttgtttca aatgctacag cttcgtctag atcagcccat gcaatggctc gtgcatcttg
    12361 taccccagga aaatctcgac ctggttcaat atagtctgct acataaatga ttttgtcaag
    12421 gagactcatc ttagcagccc cagtcgtatg cacccggatt gcatgaagaa tctctgcatc
    12481 ggtgattcct aattcccgct ctacaaacga agcgccaaca agaccatgcc agattgcatt
    12541 attataattc aataactccg gatcaaaacc gtctcttacg attaccattt tgaactcctc
    12601 atctggtcgt tcttttgcat aatcatgtgt caatgcagca atactagctt tttcaggaga
    12661 agcaccgtat tttttggcaa gagccacggc tgtttcttct actccaagca catgcttgaa
    12721 gcgttgttcg ctcattctca tctgtatttt ttgcatcagg ttttctctag aataagcagt
    12781 atattcatta ctatagtcca tattcataca acccttcatt ttgaatataa tcaattactt
    12841 tatctggaac aaggtagcgg atagaacagc cttctttgat cttttgacgt attttcgtgg
    12901 aactgatgtc gatctcaggt acatctaccc aaattacagg atatggagta tccgttgtgt
    12961 atcctggtcg acgtataccc acaaagttga ccatagaagt cagctcatcg attttatacc
    13021 attttggcaa atattctacc atatccccac cgataataaa gtaataatct gtatccggat
    13081 tgttttgcgt caattccttc atagtatcat aggtgtaact tttcccacca cgagctaatt
    13141 caatcgtttc aatctgcaaa aaaggattgt cttccaccgc taattccaac atgttgagtc
    13201 gatgttttgc atcaatcgtc tgtttctcat caacatgagg tggctgataa gaaggcatca
    13261 aaaatacgcg gtcaagacct aaattccggc cagcttgttc agccatcact aaatgtgcaa
    13321 ggtgaacggg attgaatgtt ccgcccaaga tccctacttg ctttttcttc tctaagaatt
    13381 gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt cttagcttgc gtattcatca
    13441 tggtcactcc ttatattgct tttacttctt ttgatagcct ttgatacttt tcttttgtgg
    13501 aaggtttgta taatacaata acacgtccaa tgatttggac gatttcgcaa tctatttctt
    13561 tttctaatac ttgagctgct tcttctgcaa tctcatctgt attttgaagt aacgatactt
    13621 tgatcagttc tcttttttct aaagcttctt caatttgaac gatcattgct gaattgatcc
    13681 caccttttcc gatttgaaaa atcggctgaa gatgatgcgc tttgctacgt aaaaaacgct
    13741 tttgttttcc tcttaaactc ataattttcc ttcttcctta aatcaatgct ttgcgtttca
    13801 atacatctac accctttggc gcccagcccg caattactac tggcgctgtt acagtgatcc
    13861 agcctagacc ggcaaaaacg atgtctgttt tttctttcac agaaaactca aaacgaacaa
    13921 gctccggaaa tgcctcgact tcatcaggac gaggaggctg taataatcca cctacatgtt
    13981 tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg aatatttaca tcatttgaca
    14041 cataagcaat aaatgaagca cgattgccgc tgataaaatc aaatcttgca agtccgccca
    14101 agaaaagtgt ttgcccttca ttcagctgat aaactttagg tttgatttct tttaacggtg
    14161 caatgatccg caaatctttt ttacctaagt agtgcgccat ttgatggcga tggataattc
    14221 ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat ctcgatttta tccaaggtcg
    14281 ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc cgctgtattt ttgatgattt
    14341 gattgataag cgtcgatttt ccgacatttg ttactccgac gatatagaca tcccgtccat
    14401 cgcggtattc ttcgattttt tctaatagtt ccgtcatctc atgagatttc ttagcactag
    14461 ttaataatac atctactgga cgaattcctg cttcgtaagc acgttccttc atccattgtg
    14521 ttaatttagt ttttttcaat gatttaggta atatatccac tttgttccca actaaaagta
    14581 ctggattgtc accgacaaaa cggtgtagtc ctgggattag tgaaccattg aagtcaaaaa
    14641 tatccaccac attgacgatc aatgcatctt tttgccctaa ttcattcagt aagcgcaaaa
    14701 agtcgtcatc cgtcaatgag acatcttggg tctcattata atggcgtaat ctgaaacaac
    14761 gctgacaata gacttccccg gaagctaaac ctttttccaa agccgtttgc ggcgtgtatc
    14821 ctaattcacc gggtttgttt gtttgtataa cggcaccaca gccgatacat tgtaattctt
    14881 ctgtcattcg attccacctc gccagcccat atcagggtgt tttgctaata gataggccat
    14941 aattcttttt tcccaaaaac gattaaattg tgtcttccaa ctatctgttg cgataatggg
    15001 ttttacaagg atgctacgta tccctgcacg attggctcca cgtatatcag tcataatctg
    15061 atcgcctacc attactatct catcatctgc taaatccaat tgtttctttg ctatgttgat
    15121 ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa agatcaaatt tttcaatcgc
    15181 tcgcgcgaca cgttttgaat tattgtttga aacaacaaca acagggattc ctgcattttt
    15241 catttctaaa atccaagtaa gcaactcttc tgttccatct ggattattcc atgcaatcaa
    15301 cgtattgtct aaatcggtca atactgcttt gatgcctaga ttctttaatt gggcaggtgt
    15361 aatctgatag atggcttcta ccatccatgt tggtttgtaa tttgaaaaca tgctgtctcc
    15421 tcatctgcaa aaaatgaagg agcacataaa gtactcctca accccgtatt atactgcata
    15481 tcagcaaaaa aaactacctc tatactcgtt cgtctttcag aaaaagcatt tctttacaaa
    15541 atgaccaaaa ataaagattg atcacagaaa aatttagaaa caaaaaacaa ctaatattgg
    15601 cggaacctct tgtatcgttt ttccagtaat ttatgctgat ctaatcgaac aagttccgtc
    15661 aatctttcaa tcaacgcttt tttcataatg tgtgcgactt tatatcgtgg caactgctcg
    15721 ccgttgaacg tttcaggaat aactcgatca acgattccta actcgcgtag ttctggcgca
    15781 gtaattttca tcaactcagc tgcttctgcg gcgcgactac cgtctttcca caaaatcgac
    15841 gcaaatcctt ctggagaaag cacagcataa atcgtttctt ccagcatcca gacttcatcc
    15901 gctaaaccta gagcaagggc accgccactt ccaccttcac caatgatgat ggcgatgatc
    15961 ggtaccttta aatcagacat ttcgagcaga tttctggcaa tagcctctcc ttcgccgcgc
    16021 tcttctgctc ctacaccgca atatgcacct gctgtattga tcaaggtaat caccggtcta
    16081 ttgaactttt ccgcttgttt catcagtcgt aaagcttttc gatagccttc aggatgggga
    16141 gaaccaaaat ttctgcggat attttcctgc agatcccgtc ctttttgaat accgatcact
    16201 gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa tcgcttcgtc atctgcaaat
    16261 aaacgatcac catggcattc ctcgaaagaa tcgaagatct cttcaaaaaa atccaagctt
    16321 gtccatcgtt ctttagctct tgctaactga acgacatcat gtgctgtctt atccacgatc
    16381 tatccatcct ttctgtgtat gcaattcaag aagcgtatgg attttctgtt tcaattccat
    16441 gcggggaaca atctgatcga caaaaccatg agataataag aattcagcct tttggaaatc
    16501 ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt cttccagcaa aaccgatcaa
    16561 gctttggggt tctgctagaa taatatcgcc ttccattgca aaactggcag ttactcctcc
    16621 ggtcgtcggg tccgttaaaa cagttagata aaaaagacct tcattgctat gccgtttgac
    16681 agcagcagag atttttgcca tctgcattag agaaaagatt ccttcttgca tccgtgcgcc
    16741 acctgaagct gtgaacaaga cgactggaag actttccttt gttgctcgtt cgaataatct
    16801 ggtgatcttt tctccgacaa tcgttcccat acttcccatg ataaaattag gatccatcac
    16861 tccaataatg aagggaatcc cctgaatggt tgctttacca gtgaggactg cttcatgaag
    16921 tcctgtttta tcttgcattt ttttaatttt ttccgtatat tcgggaaatg ctagcggatc
    16981 tttggttaca agatccgtat cccattcttc aaaacttttt tcatcaataa tcaaagctag
    17041 acgctgccat gcaccgattc gaaaagcgta tccgcaatga gggcatactt tttcttcacc
    17101 gatgtctttc gtataaagga tatgtttgca atttggacac tttgcccaca tgttatcagg
    17161 aacagatggc gagttagcag gtttgctctc tctattagga ttgatccgaa tatatttctt
    17221 tttcttaaac agagccatct atgcgcctcc tatctccgtt tctggtgtcc aattcggtaa
    17281 aaattcttct tgtaaaaatg ctgtgctgta atcacctgct atgactgcag gatgactaat
    17341 caaatccatc tgaaattctg cgttcgtgat gattccttct gtgataaatt cacttaaggc
    17401 tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca tggacgatta tttttgcaat
    17461 cattgaatca taatatggcg ggatcgtata tcccgggtaa actgcactgt ctactcgcag
    17521 tcccatccca cctgctggaa gcagtaagtt ttggatcttc cctggagaag gagcaaaatg
    17581 gaaagcagga ttttctgcat taatccggca ttcgattgcg tgtccttgga atactacttc
    17641 ttcttgacac acattcaaag gctcaccggc tgctatctcc acctgttttt ttactaaatc
    17701 gattcctgtg accatttctg ttactggatg ttccacttgg attcgagtat tcatttccat
    17761 gaaataaaaa tcgccttctt catccatcag aaactcgatc gttccggcat tttcataatt
    17821 gactgcttta gctgctcgaa cagcagcgtc tcctagtgct tctctttttg tttgtgagat
    17881 cactacagaa ggagattctt ccagtacttt ttgattgttg cgttgtaaag aacaatctcg
    17941 ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag atctgaactt ctatatggcg
    18001 agcaggataa atgatttttt ccaaatacat atcatcattt ccaaatgccg ctgctgcttc
    18061 ttgctgagct gaagaaaaat gctgtgggag ctcttctttt gattgaactt tccgaatgcc
    18121 tttcccgcca ccacctgcag ctgctttcag catcactgga taaccaattc tgtctgctat
    18181 ttccaatgct tcttctactg tagataatac accagtactt cctggaatga ctgggacatt
    18241 tgcttcttgc atcaattgac gcgcattgat cttatttccc attgcatcga tcgttgcact
    18301 tttaggacca ataaatgtga tattgcactc ttcacacatg gaggcaaatc ggctattttc
    18361 agataaaaaa ccaaatcctg gatgaattgc ttcggctttt gtcacgattg cagcgctcag
    18421 tacttcctga acattcagat atgaatcaac ggcttttgcc gggccaatgc agattgcttc
    18481 atctgctaac tgtgtatgaa gtgcttcggc atctgcttct gaatatatcg ctacggtttg
    18541 gatccctagt tcgcggcatg cacgaatgat acgtacagcg atttcaccgc ggttagctat
    18601 caatacttta gaaaacatat ttcacctatc caatcataaa ggttaattcc gcttcggcta
    18661 cttttttacc gtctacttta gcgattcctt tgcctattcc ggctgaagtt ttcactttca
    18721 atatctcaac ttcaaggtat aaggtatctc ccggtgtaac tttcttacgg aattttgctt
    18781 tgtccaatcc gccgaaataa gccgtctttc ccttgaattg ttcaagagaa agtaatgcaa
    18841 ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc tggcataaca ggttcttctg
    18901 gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt tttcttagca acgatccgtt
    18961 ctccttcaac catttcttcc actcgatcaa tcaaaagcat tggataacga tgtggaatga
    19021 tttcttttat ttcttgtatg ttcattgttt tccctctttc ctgctttgct tatttttgct
    19081 gaatcctaaa gagtggctgt ccatattcga caactgactc gttttctaca aggatttctg
    19141 tgatcactcc gtcggcctca ctagttatct cattcatcag tttcatcgct tcaataatgc
    19201 agataacttc tcctttggca acttgatcac ctactttttt gaaagctggt ttgtcgggcg
    19261 ctggttgcag ataaacgatt cccacgattg gcgaaacaac ctcgattcca tcaaatactg
    19321 ccgattcttt ttctttcatt atttctggag cagttgttgc ttgttcaggt atgacattga
    19381 ctggctccga aactggcaac gtttcgtttg tttgaaccat attcatgcct cgacttgttt
    19441 tatttttatt catatacaat tcaaattgac cttcacgcaa atcgaattcc gttagcgagg
    19501 attgatcaaa ttgactgact aattctctga tttcattgat atccaaaatt attcctccca
    19561 gcgtttcatg cacagaaccg cattatgtcc gccaaaccct aatgaattac tcattgcata
    19621 agtaaaactt gcaggtttgc tttcgttttt gacaactgtc aagtcaatct caggatcttg
    19681 tttttctaca ttgatcgtag gtgggataaa ttgatgttct aatgccagca aagttgccaa
    19741 ggcttcgatc ccgccggctg ctcccaataa atgtccagtc atcgatttcg tgctgctgac
    19801 gtaagtatct ctgaaatttt cacctaatgc gatttggatt gcttttgctt ctgcctggtc
    19861 attggctggt gtacttgttc cgtgagcatt gatatatccg acttgttccg gctgtacacc
    19921 agcttcttcc atcgcaagct tcattgcttt agatgcacca gaaccatctg gagtaggagc
    19981 tgtcatatgg taagcgtcac agtttgcacc gtatccaacg atttctccat agattttggc
    20041 accgcgagct tgtgcatgtt ctaaagattc aagaactaga acgcctgctc cttcacccat
    20101 cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca cgattaggat cagttgattc
    20161 tgagagtgct gttaaagctg caaatccagc aatccccatc tcacaaatcg ttgcttctgt
    20221 tcctccagca agaatgacat cagaatagcc atgtttgatg ttacggaatg cttccccaat
    20281 tgaattcgtt ccgctagcac aagctgttac gatagctgta caaatacctt ttgcacctac
    20341 acgcaaagca atatttccag ctgccatgtt ggcaatcgcc attggtacga acattggaga
    20401 aactcgtttt gcaccttttt catgtagacg aataacttgg ttttcgattg ttggaagacc
    20461 accgatccca gatccgatga tgacgccaaa ccggtcttga ttcatttttt ctttatctaa
    20521 accgctcatc tccattgctt ctaaagcagc gtagattcca tagatcgaaa acatatccat
    20581 tcgtttgcta tcttttttaa taaaatattt atcgtaaggg aaatctttga cttcacccgc
    20641 taaggtaatc cctgtttctg aagcatcaaa tttagtgatc ggtgcgatgc cattcgtccc
    20701 ctcttttaag ctattcaaaa agctgtctgc gtcatttcca ataggtgaag tgactccata
    20761 gccagtaatt accactcgat tcatttgttt gctctccttt tcttatccgt gcatgaccaa
    20821 tccgccatcg acattgatga cttgtccggt gatatatgga ttttgtgcta agaaaactgc
    20881 tgtttgtgca atatcttcta cttgtccgaa acgctgcatc ggaatttgct tttccgcttg
    20941 ttccttgact ttgtctgcta aaacttccgt catatcagtt gtgatgaatc ctggcgcaat
    21001 ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc acagattttg ttaatcctac
    21061 tacgccagct ttactagctg cataatttgc ttgaccaata tttccaatca acccagatac
    21121 actagagaga ttaatgatgg ccccttctcg ctgcttcatc atttttttca atacgtgctg
    21181 cgtcatattg aaggttccaa tcaaattgat atccagacat tttttgaaat cttctgcatc
    21241 cattcgcatc acaagtttgt catttgtgat tccggcattg tttactaaca catggatcga
    21301 tccaagcttt tcttctgctt ctttgatcat ttgtcccgct ttctcataat cagagatgtc
    21361 accggaaact cctacgcatt ttacaccgaa tgcttcgatt tcttctatct tttcttttgg
    21421 aatctctccg cgaccattca aaatgatatt tgctccagct tttgcaaaag ctaaggccat
    21481 cgctttgccg attcctcttg tactacctgt gataaataca ttttttcctt ttacatccat
    21541 aacgttcctc cttatagtat tgctattgtt tcatcaaatg tctgcttatt ctcaacacgc
    21601 agaactggta ttgttttatc gatttttttc ataaatccgc ttaatacttt tccggggccg
    21661 acttcgatga cttgttcgat gccgattgtt tttaacttat ggatactctc atagaaacga
    21721 acaggttgca tgacttgctg ttcaagtagt cccgcgattg tttctttctt cattatttcc
    21781 gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga attgaatctg acttaggtcc
    21841 ttcgccagtt tttttgctgc cggttctaaa atagctgtat gaaaaggccc gctgacattt
    21901 agagggatca ttcttttgaa ccctttttct tttaatagta aaacagcttc gtctactgct
    21961 ttttcttcgc caccaatcac gatctgctga ggtgtattat aatttgcagg agatacgatg
    22021 ccgtacttgc tagcttcatg acagctttcc tcgatcgttt caatcggcgc attcatcact
    22081 gcaaccattt tcccgcttcc agctggggca gcttctgtca tataagcgcc tctttttgct
    22141 actaacgcaa ctgcttcagt gaaggatagc gcattactag ctactaatgc cgaatattct
    22201 cctaaactca gtcctaaagc tgcatctggg atgattccat gctccttcaa taagcgataa
    22261 tacgcgatgc tgactgttag aatagctggc tgtgtatatt gcgtctgatc caaacgttca
    22321 ttttctgtaa agcaaagttc agccatatcg tagccgagaa tctcacttgc ttcatcaaat
    22381 gtctgcttta caacggcttc ttcatataaa tcttttccca taccttgata ttgtgcacct
    22441 tggccactaa ataagaatgc tgttttcatg tttttctcca atatatccag aatatatgtt
    22501 tgattcttcc tattcaaaaa ggccgagaca ttcctatttc aggaaatgtc ccagccccca
    22561 acctaatttt aatgattatg cagtttgttt ttctacgtaa gtcactaaat cttgaaccgt
    22621 tttgattccg tcttctgttt cgattttcac gtcaaattca tcttcaattt cattgatgat
    22681 ttggaataaa tccaagctgt cagcttctaa gtcttcttgg atgtcagtag tcaatttgat
    22741 ttcttcagtt tcttttccta attcttctgc gatgatttct tgaatttttt cgaataccat
    22801 aataatatat tcctccgata ttgtttgctt tttattttta taatttaaca gtctacaact
    22861 ccatgagaat acttccccat gttaaacctc cgccatagcc tgtaaatatt actttctgct
    22921 gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc caataagata gggatgcttg
    22981 cagcagatgt attcccatat ttatccatat tggtcaggaa cttttcttgc ggcatcttta
    23041 cttttcgggc aattttctca ataattctga ggtttgcctg atgcaaaagc agataatcaa
    23101 ctggtttgtc tttcatgact tcttggatat tgtttgcaac gtctcgtacg gcaaaatcaa
    23161 aaatatctct gccagtcatt tgcaaataat acgaaccttc tgaatcttct gaatagaacg
    23221 gatttttatt ttgctggtag ccagcggtca atgagctact tctggtaccg tcagctgcta
    23281 atttttcttt cagcaatttt ttctgtggat tcgcttctaa gagaatccca cctgctccat
    23341 cgccaaacaa cacagcagtc gtacgatcag accaatcgat tagcttagat agtgtttcac
    23401 caccaatcac cagtccctta gttgagccgc agcggatcat tttttcagcc atgcttaacg
    23461 cataaacaaa tcctgagcac gcagcactga tatcaaaagc gatagcatga gttgctccca
    23521 atttcccttg tacttgtgct gctacagatg gtgtcgtgta atcgggcgac attgttgcta
    23581 ctaaaataaa atccaaatct ttagggttta gatttttctg ttttatcaat ttttccgcca
    23641 ctaaaatgca taaatcagaa gtctcctgat ttgttacaca tcttcgttca cgaatacccg
    23701 tccttgacga aatccattca tcagaggtat ccatcatctt ggaaagatca ttattagtaa
    23761 taatcttttc aggaaggacg ctggctgttg cagtaatcat tccaaattgt tccattaaat
    23821 atcctcactt atagtcttgc aagaagtcgt gcaagttttt cagtgcatcc agcaaggcat
    23881 gctgttcctc cgtagccatt ccgtctaaga tgttcttgac catttcacga tgaaaatgct
    23941 gatggacccg aaatagtaac tttccttttt tcgttagtcc caactttaca acgcggcggt
    24001 catcttcact gcggatacgt tcgacatagc ctttttttac taacttgtta atagcagtgg
    24061 tcaaggtccc aacagtaatt gatagttctt tggctacttc tgaagttgtt tttttcttgt
    24121 acatcccgat tgcctcgatc gtgtgcatct ctgtgattga cagatctttg aattgagatt
    24181 ttttcagctc agactcttcg attgtcaaaa tatcattgaa gacactaaca agataatcat
    24241 ttactgtttt taaatctggt tccatgttcc accgtccact aattagtttg attatcaaat
    24301 cttttgatgt tcaaactata tcttaacttt tttcaaaatg caac
//
LOCUS       DACEMO010000021        24272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEMO010000021 DACEMO010000000
VERSION     DACEMO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.021"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..572
                     /locus_tag="IW728_000648"
     CDS             <1..572
                     /locus_tag="IW728_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1024028.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="IW728_000649"
     CDS             576..1217
                     /locus_tag="IW728_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024029.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="IW728_000650"
     CDS             1214..2131
                     /locus_tag="IW728_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1024030.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="IW728_000651"
     CDS             2128..2799
                     /locus_tag="IW728_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024031.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="IW728_000652"
     CDS             complement(3340..3828)
                     /locus_tag="IW728_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ1024032.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="IW728_000653"
     CDS             complement(4029..4295)
                     /locus_tag="IW728_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1024033.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="IW728_000654"
     CDS             complement(4295..4558)
                     /locus_tag="IW728_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1024034.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4551..4823)
                     /locus_tag="IW728_000655"
     CDS             complement(4551..4823)
                     /locus_tag="IW728_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024035.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5034..5219
                     /locus_tag="IW728_000656"
     CDS             5034..5219
                     /locus_tag="IW728_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1024036.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="IW728_000657"
     CDS             complement(5361..7184)
                     /locus_tag="IW728_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1024037.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="IW728_000658"
     CDS             complement(7782..10487)
                     /locus_tag="IW728_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1024038.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="IW728_000659"
     CDS             complement(10793..11740)
                     /locus_tag="IW728_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1024039.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="IW728_000660"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="IW728_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1024040.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="IW728_000661"
     CDS             complement(12375..12737)
                     /locus_tag="IW728_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1024041.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="IW728_000662"
     CDS             complement(12748..13869)
                     /locus_tag="IW728_000662"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1024042.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="IW728_000663"
     CDS             complement(13885..14235)
                     /locus_tag="IW728_000663"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1024043.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="IW728_000664"
     CDS             complement(14365..15876)
                     /locus_tag="IW728_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1024044.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="IW728_000665"
     CDS             complement(16198..17550)
                     /locus_tag="IW728_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1024045.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="IW728_000666"
     CDS             complement(17703..18779)
                     /locus_tag="IW728_000666"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1024046.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="IW728_000667"
     CDS             18975..20720
                     /locus_tag="IW728_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1024047.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="IW728_000668"
     CDS             complement(21000..21437)
                     /locus_tag="IW728_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1024048.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="IW728_000669"
     CDS             complement(21421..22794)
                     /locus_tag="IW728_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024049.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="IW728_000670"
     CDS             complement(23116..23604)
                     /locus_tag="IW728_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1024050.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="IW728_000671"
     CDS             complement(23626..24228)
                     /locus_tag="IW728_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1024051.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6960 a         5166 c         4486 g         7660 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agcccaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgcc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctt caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa acttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc acggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc tt
//
LOCUS       DACEMO010000022        23502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEMO010000022 DACEMO010000000
VERSION     DACEMO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.022"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            196..468
                     /locus_tag="IW728_000672"
     CDS             196..468
                     /locus_tag="IW728_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024052.1"
                     /translation="MIEEKKKVLYSNKPEFKKLVRQYAKKNIGRSITYDTFIKWLDKY
                     GYDLSQYDTCWQAVFKSLLQRNFQIDIAYRKTKECQLITVFQLNKS"
     gene            complement(579..998)
                     /locus_tag="IW728_000673"
     CDS             complement(579..998)
                     /locus_tag="IW728_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024053.1"
                     /translation="MKKSHIVTLATIFLLCVAAFFFFTNSPKNNKHITARTATTKSTI
                     KTETVYPSLSELKQLQGSDIEILGKTQITTVLFQNKNMAILTTENNKEIQLPLIVTDK
                     KNNLFEIPSLTYSKKNLKVGEVFGLAKKDNHYLAYKL"
     gene            complement(1044..1286)
                     /locus_tag="IW728_000674"
     CDS             complement(1044..1286)
                     /locus_tag="IW728_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024054.1"
                     /translation="MNITNIEELKEWIETNLVTKQEAIKITEQSPKGFQQSVRTNVIK
                     PFYESKEGKGPSVVRLYLKSDIETYAKQLKEKKQKP"
     gene            1437..2330
                     /locus_tag="IW728_000675"
     CDS             1437..2330
                     /locus_tag="IW728_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1024055.1"
                     /translation="MKLLKVLAVGVTVGGIALAINQEKVSAEEWTPRTVEQIKADIKG
                     NEYTIVWGDTLSGISQATNITVQKLADINKIANVDLIYAGNKLVFEGNVATVQNSAGE
                     TVAQTVIQDTDKVNPTQPVGQPAVSQDTTSNATNSADQSVAPTTPAGDSGANTPEQPV
                     QPSTPSDNGNQGGTVTPTPDQNQGGNNNQDNQGNQGGDTGNNGNQDNQGNNNGNNNNG
                     NNNGGNEGGDVTPPQPVEKWTVWYSASDEANAAHNIEKGTKVFDTEAEATAWIDQYAD
                     GLLAQGITSNSYGVSSFLVQE"
     gene            2582..5692
                     /locus_tag="IW728_000676"
     CDS             2582..5692
                     /locus_tag="IW728_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ1024056.1"
                     /translation="MILGALAMFLGFGLSSQSVNADNITMKPGEKGDIYYDASNVYTM
                     HGTKHGQPWSDATAAMAVSPNNGATDRIVFCIQPGVPFINTNNPDYEAVAVTDIPKDA
                     QIASVIWNTFYAFGTNFTNEDRIVTQAVIWELLPQYGIKVDSISGIPDFQAKKQKLYD
                     GIKEYKRLPEFNNKTIDLTFGETKKLTSSVDLNSFETQVSNSAKVNWNIAADGKSVDV
                     TPTDPNVTGGSISFKRSYMEGTPIALEKTGSQTVYLPSVKDPNNYSVNFRIQTVGDIT
                     IKKVDKETGEVVPNTQFKVEFSGKGAPSAQTVTTDKNGLATIKGINHGVHVKATEIAV
                     PAPYVLGSAIGDSDVVEGDVVAGQTVTLTQRNQKAKGQILIEKSGVETGKDMWNANYT
                     LAGNVFEIHEGNETGTVVRTITTDDKGHAETGKDLSLGTYVITEKTASNGFANTFKPV
                     TVKIEYANQTTAVIIKGTEGTNQEVTGSTLLTKEDAETGKETQGRATFDGAQYGLFHK
                     DGTPVKWSENFKPTTSKGNKLEGDEVKFELSDKEQQASVEHLALGDYYWQELVAPEGY
                     QIDNTKREFSITYKDQDTKVIETQSTSQENVIKFSLDGFKYVDSKSGDTKTGYNGIDF
                     TLTPIDPTKGEQRKVTTETDENGYDGYWAFNEVPYGDYKMSEVKAPEGYKTIKDLIIN
                     SKFDAEKREYTFTITEDGQKEPIKTLTVPEAKINEGSNVIHLSKLFLTNKVKEMPTIR
                     TKATVDGEKTFTPSKETPMQDVATITNVEKGVEYTNKIKLWRIVNDDYDNAKVVWEGS
                     HDFVAEGDKAEDVIKQMVDTSKDDNTVSYVFTEELFIKDGAKVAEHNDLTNKEQTIRP
                     VVPELPTIETLFVTKDGGKTFDPTKDQTLIDEVKATVPKEDIGQTFYYVTQFHKIDKN
                     GKVTVVGTDESEHKAGEMTYEFKAVYNYKANTLKDGEKLVATHIAYTDKEHKNEYAKH
                     FDLKNEKQTLTAKTPVTPATPTTPTTPQKSIPQTSGSLVNIGSLIILGIAAVAGAALY
                     VTTKKK"
     gene            5758..6795
                     /locus_tag="IW728_000677"
     CDS             5758..6795
                     /locus_tag="IW728_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1024057.1"
                     /translation="MFLGKGEINIVKKIIKALPLLWLLSSLILAICGFYGYTVLLRPE
                     NTIKQAQQITHPIQSEKEIKEIQQTTIGKATYNENQIQPVTPTDYAEAQLSYEALVNQ
                     WGIGSLYIPSSGIYSKILAGMSNDNLMVGLGTYYPNQLLGKGNYVLMAHNLVQGGGVL
                     RNLSQSSVGSTIYATDFSKIYEYEITTNKIVNQSESKLLDIPQEGDSPLMTIFRCEGG
                     LHTANRALIQARYVRSYSAENGSHDIKQALGLETTRNKTVNKQRLNDQQATSTNRTEA
                     AKESITPDKDTKKAKQTNKIEWRFTEKKAIYSNFQVFSILVFQLANAYPILIGLVFLV
                     GLSSCILLNRI"
     gene            6882..8408
                     /locus_tag="IW728_000678"
     CDS             6882..8408
                     /locus_tag="IW728_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1024058.1"
                     /translation="MKKKHISKVASAVLLSQMLLTTPLQVLADEQLSTPTSQSSTMNN
                     QSEESTSSSDEQTGTSTTQTTTDSTESSTDQSTTDDTTTSSTDTTSTTESSEKPTEDS
                     TTDSTSTTESSSESKPDETKPSEIKPSESKPEKPNTGSSTNESQTKPTTQPSNNQQAP
                     SVDPNNQATATNEGQQAKPAESESKPYHQTPTAPIQELVPSSKETAEGNIHFEKDESV
                     ESFVRKIGESARKVGQENDLYASIMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGK
                     GVSFATQEDLGDGTLYTTQATFRQYENYEDSLNDYAQLLKEGLTGNSQFYSGVWKTNA
                     KTYQEATKFLTGRYATDTKYDQKLNGLIETYHLTDYDKEVAGPQLSKEGYIVPVKNYT
                     ISSPFGTRGGEFHRGLDLAAAQGEPIYASKAGTVVKAEFHPSWGNYVAIEHEDGTTAL
                     YAHQQEYQVKVGDKVKQGQIIGYVGSTGNSTGSHLHFELCLDHSLSQSQLVDPETVLF
                     "
     gene            8587..8994
                     /locus_tag="IW728_000679"
     CDS             8587..8994
                     /locus_tag="IW728_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024059.1"
                     /translation="MTNRNEGITIYLTIGKEVKLNFDSENNLSSIRLSINKVAELFGN
                     KVVNEESIFYVSTVALVTKSNYKTFISVVGGKQVISGDAVYEEVMIDQERTSNKRGNK
                     NVYEQGYFEFFVSEEDKIPKEKLILPYNVKKRR"
     gene            9111..10172
                     /locus_tag="IW728_000680"
     CDS             9111..10172
                     /locus_tag="IW728_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAQ1024060.1"
                     /translation="MTFIDRKDNRVRQLFNMELIRAETKWYSFYQKNHRPPDYNFLQE
                     EITSFGVNRLPMFDRRYEGRVNDELSVDNFVHYHIQQLKLEKILSPVFFEKNDLRFME
                     RYAKNKAKAYFNERDQYIKGYESFRISRNKIMQRAGYEQLKYEFVSDNMVQGYFKEEV
                     AKMAANDERVAEFVFDEGKSLMRNLTQKMSRGGVSIINGSTKAVTNQIKKQRTTGKQR
                     YNQLKKHGALEHIDVSKTDLKPLANELKKYKVDFAVKRNKETGKIHVFFKAQDRDTLN
                     LALENVLQKFTNEKEQNREQQQEKPNKDIEKSSVDDKDKDKSLTKKLDKLEDKAQEIN
                     QQREQLKENTKNKTKGQER"
     gene            10247..12043
                     /locus_tag="IW728_000681"
     CDS             10247..12043
                     /locus_tag="IW728_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1024061.1"
                     /translation="MVYFSIPQNTDSSWINVVNEAFDKFLEPKTFTISPFTFSKIAMK
                     GGAIIAVIVLLIDLYIISGKKNLRMGEEHGSAKWGTSKDIAPYVDKKFKNNLLFTNTE
                     RITIETKLKKNKEYERNNNVVVVGGAGSGKTRFYVLPNLLQMHSSYVINDSKGGILSD
                     TAAIMQQNGYRIKVFNLNTMKNSDGYNPFHYIRRTEGDCEIDILKVVNNLMKNTSDPN
                     KKGGDSFWDDAQRSFLAAIFHLLILKGKPEEQNLGIVADLVRRARVKEDDEEYISSID
                     LVFQELEQEQPSCFAAKQYQNFKMAAGKTAKSILISLGVRLSPFDVPDIRKLTSTDTL
                     ELDTIGDRKTVLYIILPDTHTTMNFLASMIYQQLFDTLVYKADNVYNGSLPIPVRCLL
                     DEFANTGQIPDFENVIATTRSRGISVDVILQNLTQISKKLYKDSWETIIGNCDSFLYL
                     GGNEQSTHKYISTQLGKETIDVVTYNESRGTTGSFTKNSQKQGRNLLDPNEVREIKGG
                     KCIYMLRGTKPFLSDRFKLERHPLFKKLKETPSGYMYQSPLDQEEFIANNDPIDFELS
                     KNVDMKEFLKHTEIETYDFEDEEIETINLDEL"
     gene            12060..12260
                     /locus_tag="IW728_000682"
     CDS             12060..12260
                     /locus_tag="IW728_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024062.1"
                     /translation="MGTFNAIIDILSKYAIVGGGLYAVWGAVVLGGALKDHNGPQMQS
                     GIWQIVGGALIVAAAALFKTIV"
     gene            12289..13140
                     /locus_tag="IW728_000683"
     CDS             12289..13140
                     /locus_tag="IW728_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024063.1"
                     /translation="MMDGFLTWCVESILEVISFSSKTSHLTSNLENFSPQLYKYSTTI
                     LDSVSKPIAFSILALFFALELQRISNRVETNGGGQTGFEMIFKSLVRLGLCKLAMDQV
                     SLIMRAIMDITGYMTDKINHLGMGNKEVSDMLDAKKILAPILKEGLMFKVGVSTVLII
                     ALLITIGSIVIVQVVVNMRFIELYAFLAMSPIPIATFPNDEWSSIGKNFLKSFTATAL
                     QGTLIFMVMQFYPYLLSSAFSDITNSGSASETFLMTLIGIIGNSVLLIFAVMATGRWS
                     KSITNAM"
     gene            13405..13776
                     /locus_tag="IW728_000684"
     CDS             13405..13776
                     /locus_tag="IW728_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ1024064.1"
                     /translation="MAIRVKVPKEIKDYQEKLMGGMSVRQLVFFVLAVLISVGVTAYC
                     VLVLHMSVDAAGYIVIPLLIPIVAFGWIRKEGMPLEKYGKIILNYYSKTGIRIYQTKE
                     RSNLNDFRKKRFQKVKETTKR"
     gene            13724..16174
                     /locus_tag="IW728_000685"
     CDS             13724..16174
                     /locus_tag="IW728_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1024065.1"
                     /translation="MILEKNDSKKSKKQPKDKQAKKVLKILAKQKKQRGKNQRRDAPP
                     LSSQRSLTYKAMYENGVCELKEGLYSRTFKFSDINYAIADQSEQELTFSSYCELLNSC
                     DSDTSLQISIINRSKDKKQFEKDLSYELVDDGLDVYRKEMNHMISNKIKYGNSSLQKE
                     KYITFNQRAKTLEQAIPALNRLQGDLEDQFSEMGCEVEELSGIERLRVVNDILRPNKK
                     LHFEYEDLLFSGLTTHSAIAPDSFDFKYRKDRFKMGDYYGQVLYLRNYPAELSDKLIT
                     NITDIPTNLQITLHIEPLDQQKGLDLVKTKLSFMEQQKIDEQKKAARSNYDPNLIPME
                     LRRSMDEAQELLDQMQGDNEKLFSFTFLVMAVDKDERKLDDTIEQILTAGRKNDCEFA
                     KLNYLQEEGLNSTLLWGNNFVPIHRTLTTASTAVFIPFTAQELMQPNGKYYGLNSVTK
                     NLVTLDRRKLKAPNGFILGTPGSGKSFATKKEMTAVLLSDPDAEVIVIDPEREYTPLA
                     EALNGEVIQISAGSKNHVNPMEINNDYADDDDPVILKTDFVLSLCEMLVGGNIGLSPT
                     QKTLIDRSCRFAYKKYFENGSGMPTLKDFSNILKDQPEDEARQLAVELELYTVGSLSV
                     FSHQTNVDTSKRFIVFDVKDLGKQLRSMGMLIILDQIWNRITSNRNAGRRTWVYIDEI
                     QLLFSNEYAENYFFELWSRARKWGAIPTGITQNVSTLLSSLNAERMLSNSDFVLMLNQ
                     SQRDRERLGELLHISSRQKKYITNAKEGAGLMYAGDSMIPLYDEFPKDTNLYKVMSTK
                     IEDVYKYQNEQREQGTTV"
     gene            16171..18018
                     /locus_tag="IW728_000686"
     CDS             16171..18018
                     /locus_tag="IW728_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ1024066.1"
                     /translation="MNTDFRERIIRKQKTAKKFVVNRDNNQFKKKIVSTDYTKRAKKA
                     NHSGKIKSSRGTSYLIKKDSAKNVVKDVNSKAQKNGVEQGNATAELETSKTIGRGIKN
                     STKATKTVAKTTTKVTKKSKELIQTKASKKEVLTVVKKGSKKGLVNTSKAAKDTVINE
                     IQEFRGDSESQGINEAIQLKDFIDTGISGAKKTKKAANTAKKSSKALTKFGKSSYKYT
                     QRIAEIGIRRVQQVLSKEVAKALIAKLAIPLTLAVLILSIVTAVISTVNIFNFGAGDC
                     DTSANYDNITTKDIESNAKSIYSIIRKEIPESTPQGISGALGAMQFESQLNPNAVNPS
                     SGATGIAQWLGSRLTSLKEFAHKKGKKETDLGVQVEFLLSELNSSYYQSSKKILAMTD
                     VHEACKEWVMKFEGLSQDSSQWYLDQRNSYADHWYATLGTSDPIAEGTISNGADSELN
                     TLTCDLESDGGDIMEIAKSWLGWFHYDQIHPAPDLGTDLKNPNKNGRTDCSGFVWLVL
                     NKAGYKVPANMQWFTGSMASDARGSHQYLKQISETEAKAGDIVIVNQGAGAGSNGHTG
                     ILTEKWRGKDTKIIQEGGNGDSVNIEAFGTSFMSLLDGGDVVLARPIKK"
     gene            18038..18667
                     /locus_tag="IW728_000687"
     CDS             18038..18667
                     /locus_tag="IW728_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024067.1"
                     /translation="MLNRKSATITIFVLIVSVFSLSIGLNIKQELTVKTANSKIAALE
                     KKNDILKRETKDQKSRISKLEDENNKMIETQIVNKDGDVYKEFTNIATKYFKSRFNYD
                     PKTYKESKENVRDLISEELYKKFSENQLTYGDSNNVSSRLDNLEIYSGSLINQKTITG
                     LLIVDYESKLGETDWFKNSEIYTVQYDIHAKKLTKIQSLGSVLRGDMIE"
     gene            18660..19034
                     /locus_tag="IW728_000688"
     CDS             18660..19034
                     /locus_tag="IW728_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1024068.1"
                     /translation="MNKKFFSICVLIFTVSIIFLADHSYIKYIEAQNDPVYLDESNQE
                     KIVFYRNDCRNCEKIMPYLIRHNFLYHDLTFVNMNNKENKRYIKEYGLTSVPTIISLK
                     QRYVGTDISKIKTLVMSKGDDF"
     gene            19034..19267
                     /locus_tag="IW728_000689"
     CDS             19034..19267
                     /locus_tag="IW728_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2968 domain-containing protein"
                     /protein_id="HAQ1024069.1"
                     /translation="MARKSLTERIQQKKEEATKLEKRIQEDQYKLEKLTKQIRDLEAE
                     QVLAILDNHKLSVKDLNKILSKQPDNNNFINHN"
     gene            19340..19717
                     /locus_tag="IW728_000690"
     CDS             19340..19717
                     /locus_tag="IW728_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024070.1"
                     /translation="MLIAKRILASIVAIPCFLLSIIVIAVNIISIPILLVIQILKKIV
                     ILFITLVWIGFIGLFIYGLSSLGFSETLSMFTVPTIGILILTLILSIFTFISDKIPDF
                     LGIVSSFFLSIPKFLWGAEETEE"
     gene            19738..20805
                     /locus_tag="IW728_000691"
     CDS             19738..20805
                     /locus_tag="IW728_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024071.1"
                     /translation="MFLKKKYVRFIEQRDIELPETPVEIYRGIYLLEQENDRLTDKHT
                     KGSTLILLTDKKGNELNRRLIQLPIEQGIETILTNLLIEPTSNKKQSSSKSQMILNDI
                     EEPKIKNKKKTVNYQTVFFLFTTNLAKKIYMALIIVLFLFFTSFFVLNNHSSTSTDSQ
                     SYHELLKKKDYEKIIKQYPEKENELVEKLYSEKNSKELKELAEKSDSQIALFYWDFLN
                     EHWQAVTTMKNVTPNETIQAMRGYAYLKQGKLDEAELINKEIDNQTLKDQIYQFKKQE
                     AYKALHEKDIEKAEKINKEINDSELSEDIKVAKSMVNLLQKYEKDRSDSKLSEDERKE
                     AENNYKMWSENLKQLGGNDNA"
     gene            20798..22378
                     /locus_tag="IW728_000692"
     CDS             20798..22378
                     /locus_tag="IW728_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024072.1"
                     /translation="MHNKKKFLVVGLFSAVLLGGAVTYGMYSQQPHNETEKTEQTTKS
                     ESSKHKSKKSAWEESVKGKEDKKDKNKHSTKKADDILAVIADTPLVKQQPNQNKNNLI
                     DRLAVALDDQTKKEADRQEKLIIKDKKSVDEPVLVDLSYKPQFEDTKPSNKENGKTEN
                     NKDENSESDKGNDTKPDNSLPDPLPVPEPNPLPDPTPSPDPNPIPTPDPDPDPDPDPT
                     PDPEPSIDEQINQSKEELNDSRKQANELNKQLTQVKDSLDNLQSIGASTEKNVDKSAL
                     QWQKVADLVNEYNQLTNEIKKLIEEDGTVLPINYDLYKETYEKLNQKVTEVKTAQKEA
                     TNTTNEINKNVEQATDTLNNLEETKKEYQNKQKDVSEVDSNVNSAINHAQSNEQVASA
                     VQDEINQAAQSNEQLNETNKTVGEQLNQVDPIESETDIASAKEQTADINNQAEEQNNN
                     VDDMMNDFNNLPKPSDSEEPSVPENTDQNQDSTSQPQKIDNASVSQNKLENEQVSEKT
                     LPVTNQNSNQSEIEDIQK"
     gene            22397..23428
                     /locus_tag="IW728_000693"
     CDS             22397..23428
                     /locus_tag="IW728_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024073.1"
                     /translation="MSYKDSEAKKNNKTAELLSQAEEGIKEVLSSDKYKNFLRVMSKF
                     HNYSLNNTLLILMQNPNATHVAGYKTWKNSFNRQVNKGEKSIKIIGGRPYKRKVEEIK
                     NGQKEEKEVEGISFFPVSVFDVSQTSGEPLPQLMNELSGEVKNYPELYVALKQSTNFS
                     IEFADIQGGTKGYCSPLEKKIVLNNGMSQAQTIKTLIHEITHADLHAPEFDKNSSIKT
                     TKSTKEVEAESTAFVVCEHYGIDTKDYSFPYLAAWSSDKELKELKNSFEVILKQADNL
                     IKKIDKNLAELQKEVTKEKEEKQSTLSLSDQLEEFEDKAKSLNEQRDKEKLINKIRQT
                     KEQKDVSTL"
BASE COUNT         9052 a         3452 c         4320 g         6678 t
ORIGIN      
        1 aaaatgaaaa agagcttttt cattttctaa gtgatatgaa aaggaagaac caagtagcaa
       61 acttggataa ttatatcaaa tggatcaaag atcagtcagt gcaaataaaa caagatagag
      121 agtctggtaa cttaaatatc aaaattgcag taacggataa tcaaaacaac attttaattt
      181 attaaggaga ataaaatgat cgaagaaaaa aagaaagtat tatattctaa taaacccgaa
      241 tttaaaaaat tggttaggca atatgctaag aaaaatattg gtcgtagcat tacttacgat
      301 acttttatta aatggttaga taaatatgga tatgatttgt cacaatatga tacttgctgg
      361 caagcagtat ttaaatcact cttacaaaga aactttcaaa tcgacatagc gtatagaaaa
      421 acaaaagaat gtcagcttat tacagtattt caattaaaca aaagttaacg attatgatct
      481 caaaatatca ttttatgatt ttgatacaat aatcacaata ttatatagta aaatttttta
      541 aatgaggtgc aaaaatagag agacaaatgt ctccctattt ataatttgta tgctagatag
      601 tgattatctt ttttagctaa accgaagact tcaccaactt ttagattttt ctttgagtaa
      661 gtgaggcttg gtatttcaaa caagttgttt ttcttatcag tcacaattaa gggaagctgt
      721 atttctttgt tgttctctgt agttagaata gccatgttct tattttgaaa taaaacagta
      781 gtaatttgag tcttccctag aatttctata tctgaacctt gcaattgttt tagttcactc
      841 aaagaaggat agacagtttc tgtttttatt gtactttttg tggttgctgt cctcgctgtg
      901 atatgtttat tgtttttggg cgagttggta aaaaagaaaa atgcagcaac gcaaagtaag
      961 aaaattgttg ctaatgtaac gatgtgtgat tttttcatag catatctcca ttaatatcaa
     1021 tttataaaaa gcagaaactg taattatggt ttctgctttt tctcttttaa ctgtttagca
     1081 taggtttcaa tatcgctttt tagatagaga cgaactactg aaggaccttt tccttccttg
     1141 ctctcataaa atggttttat tacatttgtg cgaacagact gctgaaatcc tttaggagat
     1201 tgttcagtaa tcttgattgc ttcttgttta gtaactaaat tagtttcaat ccattctttt
     1261 agttcttcta tattagtgat gttcatatta atcccacctt tctgtggttt tgattgtcta
     1321 cagaaatatt ataaatgaaa aaaagtaaaa aatctaatat aaaatggttg caatactatt
     1381 ccgttactgg tatagtatta atataataaa aattaaaaag aaagaaggaa taaaacatga
     1441 aattattgaa agtgttagct gtcggtgtaa cagtaggagg aattgcgtta gcaatcaatc
     1501 aggaaaaagt gagcgcagaa gaatggacac cacgtacagt agaacaaatt aaagctgata
     1561 tcaaaggcaa tgagtacacg atcgtttggg gcgacacttt aagtggtatt agccaagcaa
     1621 caaacattac tgttcaaaaa ttggctgata tcaacaagat tgcaaatgtt gacttgatct
     1681 atgcgggaaa caaattagta tttgaaggca atgttgcaac tgttcaaaat agcgcaggtg
     1741 aaacagtagc tcaaacagtt attcaagata cagacaaagt taatccaact caaccagtag
     1801 gacaacctgc agtttctcaa gatacaacaa gtaatgcaac taactcagct gaccaatctg
     1861 ttgcacctac tacaccagct ggagatagcg gagcaaacac tccagaacaa ccagttcaac
     1921 cttcaacacc ttctgacaat gggaatcaag gtggaacggt aactccaact cctgatcaaa
     1981 atcaaggtgg aaataacaac caagataatc aaggaaatca aggcggcgac actggaaata
     2041 atggaaacca agacaatcaa ggaaacaata atggaaataa caacaacgga aacaacaatg
     2101 gcggtaatga aggtggcgat gtaacaccac cgcaaccagt tgaaaaatgg acagtttggt
     2161 attctgcttc agatgaagct aacgctgctc acaatattga aaaaggaaca aaagtatttg
     2221 atacagaagc tgaagcaaca gcatggattg atcaatatgc agatggcttg ttagcacaag
     2281 gtattacttc aaattcttat ggcgtatctt catttttagt tcaagaataa taaaatctgt
     2341 ttaccaagac acttaagaaa attcttaggt gtcttttttt tacccttttt tcaaattgct
     2401 attgaagggt ttattttctt aaggggggtg attccaatct aaaatttaat gtcagtcggt
     2461 ggagcgagcc tttgggggtg ctcttttttt attgtactta tatatattga ttgaagagag
     2521 ggagattata gtgagaataa aaaacaagag aaataagtgg aaagttccca agtatctatt
     2581 gatgatttta ggggcattgg caatgttttt aggatttgga cttagttcgc aaagcgtaaa
     2641 tgccgataat attacgatga agccgggaga aaaaggcgat atctattatg atgcaagtaa
     2701 tgtttatact atgcatggta cgaagcatgg gcaaccatgg agcgacgcaa cagctgcaat
     2761 ggcagtttct cctaacaatg gggctacgga tagaattgta ttttgtattc aacccggtgt
     2821 gccttttatt aatacaaata atccagatta tgaagcagtt gctgtaactg acattcctaa
     2881 agacgcacaa attgcttctg tcatttggaa tacgttttat gcttttggga ctaactttac
     2941 caatgaagat cgaattgtca cacaagctgt catttgggaa ttattaccac aatatggaat
     3001 caaagttgac tctatcagtg gtattcctga ttttcaggct aaaaagcaaa aactttatga
     3061 tggaatcaaa gaatataaac gtttgccaga atttaacaac aagacgattg atctaacatt
     3121 tggcgaaact aaaaaattaa cttcttctgt agatttaaat tcttttgaaa ctcaagtttc
     3181 taatagtgca aaagttaact ggaacattgc agctgatggt aaatcagtcg atgtaactcc
     3241 tactgatcct aatgtgacag gtggaagtat ttcttttaaa cgttcataca tggaaggaac
     3301 accaatcgca ttagaaaaaa caggctcaca aacagtctac ttaccaagtg taaaagaccc
     3361 taataactac agcgtgaatt tccgtattca aacagttgga gatattacaa ttaaaaaagt
     3421 agataaagaa actggcgaag ttgttcctaa tacgcaattt aaagtagagt ttagtggtaa
     3481 aggagcgcct tcagcacaaa ctgttactac tgataaaaat ggtttagcaa cgatcaaagg
     3541 aattaaccat ggtgtgcatg tcaaagcaac agaaattgct gtaccagctc cttatgtttt
     3601 aggatcagct attggcgatt ctgacgtagt agaaggcgat gttgtcgcag gacaaacagt
     3661 tactttaaca caacgtaacc aaaaagctaa aggacaaatc ctgattgaaa aatctggtgt
     3721 agaaacaggc aaagatatgt ggaatgctaa ctatacatta gcaggaaatg tatttgaaat
     3781 tcatgaggga aatgagacag ggacagttgt tcgcactatt acaacggacg acaaaggtca
     3841 tgcagaaaca ggaaaagatt tgtcattagg tacttatgta attactgaaa aaacagcttc
     3901 taatgggttt gctaacacat tcaaaccagt aacagtgaaa atcgaatatg caaaccaaac
     3961 aacagcagta attataaaag gcactgaagg aacgaaccaa gaagtaactg gttcaacatt
     4021 gttaacgaaa gaagacgctg aaacaggcaa agaaacacaa ggacgtgcta cttttgatgg
     4081 ggcacaatac ggattattcc acaaagacgg tactcctgtg aaatggtctg aaaacttcaa
     4141 accaacaact tctaaaggaa acaaattaga aggcgacgaa gtgaaatttg aattatctga
     4201 caaagaacaa caagcaagtg ttgaacactt agcattaggt gactactact ggcaagaact
     4261 tgttgctcca gaaggctacc aaattgataa tacgaaacgt gaattttcta ttacttacaa
     4321 agatcaagat acaaaagtaa tcgaaactca aagcacttca caagaaaatg taatcaaatt
     4381 tagtcttgac ggctttaaat atgttgattc aaaatcaggc gatactaaga ctggttacaa
     4441 tggaattgac ttcacgttaa ctcctattga tccaacaaaa ggcgaacaac gtaaagtaac
     4501 aactgaaaca gatgaaaacg gctatgatgg atactgggca ttcaatgaag tcccttatgg
     4561 cgactacaaa atgtcagaag taaaagctcc agaaggctac aaaacaatta aagatttaat
     4621 cattaacagc aaatttgatg ctgaaaaacg tgaatacaca tttaccatta ctgaagacgg
     4681 acaaaaagaa ccaattaaaa cattgactgt accagaagct aaaatcaatg aaggttcaaa
     4741 tgtgattcat ttaagcaaat tattcttaac taataaagtg aaagaaatgc caactatccg
     4801 cacaaaagct actgttgacg gagaaaaaac atttactcct tcaaaagaaa caccaatgca
     4861 agacgttgct acaattacta acgttgaaaa aggtgtcgaa tatacaaaca aaatcaaact
     4921 ttggagaatc gttaacgacg actatgacaa tgctaaagtt gtttgggaag gttctcatga
     4981 ttttgtagct gaaggcgata aagcagaaga cgttattaag caaatggtag atacttcaaa
     5041 agatgacaac actgtctctt acgtatttac ggaagaatta ttcattaaag atggagcaaa
     5101 agttgcagaa cacaacgact taacgaataa agaacaaacg attcgtccag tcgtgcctga
     5161 attgccaact attgaaacat tattcgtaac aaaagatggt ggcaaaacat ttgatccaac
     5221 gaaagatcaa acattaattg atgaagtaaa agcaactgta cctaaagaag atatcggtca
     5281 aacattctac tatgtaacac aattccataa aattgataaa aatggaaaag ttactgttgt
     5341 aggtacggac gaatcagaac acaaagcagg cgaaatgact tatgagttca aagcggttta
     5401 taactacaaa gcgaatactt tgaaagacgg cgaaaaatta gttgcgactc atattgctta
     5461 tacagacaaa gaacacaaaa acgaatatgc caaacacttt gatttgaaaa atgaaaaaca
     5521 aacactaacg gcaaaaacac cagttacgcc agcaactcca acaactccaa caacaccaca
     5581 aaaatcaatt ccacaaactt ctggatcatt ggttaatatt ggaagcttaa ttattttagg
     5641 aattgctgct gttgcaggtg cagcattgta tgttacaact aagaaaaagt aaataaaacc
     5701 gaaaaataag aatgaagggg aagcactttg aaaaaggtgc ttttcctatt ttagttattg
     5761 tttttaggga aaggagagat caatatagta aagaaaatca ttaaagcctt accgttattg
     5821 tggctactaa gtagcctgat tttagcaatc tgtggatttt atggttatac cgttcttctt
     5881 cgtcccgaaa atacaatcaa acaagcacag caaatcactc accctataca gtcagagaaa
     5941 gagatcaagg agatacaaca aacgacaata gggaaagcaa catacaatga aaatcaaatc
     6001 caacctgtta cacctacaga ctatgcggaa gcacagctga gttatgaggc tcttgttaac
     6061 cagtggggaa tcggttctct ctatattccg tcttctggta tatactccaa gattcttgca
     6121 ggaatgagca atgacaattt aatggttggc ttgggaacct attatccaaa ccagttactt
     6181 ggaaaaggaa actatgtttt gatggcacat aatttagttc aaggtggggg agtccttcgt
     6241 aatttatcac aatctagcgt aggatcaaca atttatgcga ctgattttag caagatttat
     6301 gaatatgaga tcactacaaa taaaattgtt aaccaatcag agagtaagtt attggatatc
     6361 ccacaagaag gtgattcgcc tttaatgact atttttagat gtgagggagg attacacaca
     6421 gctaatcgtg cattaattca agctaggtat gttcgatctt actcagctga aaatggttca
     6481 catgatatta aacaagcttt aggattagaa acaacgagaa ataagacagt caataaacaa
     6541 cgtctaaatg accaacaggc gacttcaact aatagaactg aagcagcaaa ggaaagtatt
     6601 actcccgata aggatactaa aaaggcaaag caaacaaata aaatagaatg gcgcttcaca
     6661 gaaaagaaag ctatttattc taacttccaa gtgttttcta tcctagtatt tcaattagct
     6721 aatgcttatc ctattctgat tgggttagtt tttcttgttg gtctctcaag ttgtattcta
     6781 ttgaacagaa tataatgagt gacaaaactt atatatattt ttataaaaaa ttcataaaat
     6841 aaagttatct tagatgtaac acaggaaagg aaagagaaaa agtgaaaaaa aagcatattt
     6901 caaaagtagc ttccgctgtt cttctttctc aaatgctatt gacaacaccg ttacaggtat
     6961 tagcagatga acagctttca acgccaacca gtcaatccag cacgatgaat aatcagtctg
     7021 aagaatctac ttcaagtagt gatgaacaaa caggaacgtc tactacacaa acaacgactg
     7081 attccacaga aagtagtaca gatcaaagta caaccgatga tacaacaact agctcgacag
     7141 atacgacgtc tacaacagaa tcgtctgaaa agcctacaga agacagtacg acagattcaa
     7201 caagtacaac ggaatccagt agtgagtcta aaccagatga aactaaacct agcgaaataa
     7261 agccgagtga atcgaaacca gaaaagccga acactggcag ttctacgaat gaatcacaga
     7321 ctaagccaac aactcaacct tcaaacaatc agcaagcacc gtctgttgat ccaaacaatc
     7381 aagcaacggc aacaaatgaa gggcagcaag ctaaaccagc agaaagcgag tcaaaaccgt
     7441 atcaccaaac gccaactgcg cctattcaag aattagttcc ttcaagcaaa gaaacggctg
     7501 aaggaaacat tcattttgaa aaagatgagt ccgtagaaag ctttgtccga aaaatcggcg
     7561 agtctgctcg caaagtagga caggagaacg acttatacgc ttctatcatg attgcacaag
     7621 ccattttgga atcagctagc ggtcaaagtc agttagcgca agcgccaaat tataacttgt
     7681 tcgggatcaa aggaacacat aacggaaaag gtgtttcttt tgctacacaa gaggatttag
     7741 gtgacggcac tctttacacc acacaagcta cttttagaca gtatgaaaac tatgaggata
     7801 gcttaaatga ctatgcacag ctgcttaaag aaggtttgac tggtaatagt caattttata
     7861 gtggtgtatg gaaaacaaac gcaaaaactt atcaagaagc aaccaaattc ttaactggca
     7921 gatatgcaac agatacaaag tatgatcaaa agctaaatgg tttgatcgag acctatcatt
     7981 taacggacta cgacaaggaa gtcgcaggac cacaattaag taaagaagga tatattgtac
     8041 cagtgaaaaa ctatacgatt tctagtccgt ttggtactcg tgggggagaa tttcatagag
     8101 gattagattt ggcagcagca caaggcgagc caatctatgc cagcaaagca ggaacggtcg
     8161 ttaaagcgga gtttcaccct tcatggggga attatgtagc tattgagcat gaagacggta
     8221 caacggcttt atatgctcac caacaagaat atcaagtcaa agttggcgac aaagtaaaac
     8281 aaggtcagat cattggctat gttggctcga caggaaatag cacaggtagc catttacatt
     8341 ttgaattatg tttagatcat agtttaagtc aatcacaatt ggttgatcca gaaacagtct
     8401 tattttaaaa ataaggctgt tttttttatt caaattttaa ggaggtattg tctatgttgc
     8461 tatttataag tggtttattt ttaggcggat ttattggtgt ggcgttaatg tcattagtag
     8521 caattgatcg ttatgaagat atgattgatg aaagaagata attaagaaag agggagtaca
     8581 aattttatga cgaatagaaa tgaaggaatc actatctact tgactattgg taaagaagtt
     8641 aagttaaatt ttgattcaga gaataactta tcaagtattc gtctatccat taataaagtt
     8701 gcagaacttt tcggaaataa agtagtcaat gaagaaagta tattttatgt ttcaacagta
     8761 gccttagtga caaaaagtaa ctataagact tttatttcag tagtaggtgg aaagcaagtt
     8821 attagtggag acgcagttta tgaggaagta atgattgatc aagaaagaac tagcaataaa
     8881 agaggaaaca aaaatgtata tgagcaagga tactttgaat ttttcgtgtc agaagaagac
     8941 aaaattccta aagagaaact aattttgcct tacaacgtaa aaaaacgtag gtaaaaagct
     9001 tatgtgagga tatatctatg aatataacag taaaagaatt aggcgaacct atagaactcg
     9061 aagattattt agaagaatat aaattccaag tcatattttt attaaaaaat atgactttta
     9121 ttgatcgtaa agataatcgg gtaagacaac tttttaatat ggaactgatt cgagcggaaa
     9181 ctaagtggta ttctttttac caaaaaaatc accgtccacc tgattataac tttctacaag
     9241 aagagattac gtcatttggt gtcaatcgtt tgccgatgtt tgatagaaga tatgagggac
     9301 gtgtgaatga cgaattaagc gtagacaact ttgttcacta tcatatacaa caattaaagc
     9361 ttgaaaaaat actgtcccca gttttttttg aaaaaaatga tttacgattc atggagagat
     9421 acgcaaaaaa taaagcgaaa gcttacttca atgaacgtga tcaatatata aaaggctatg
     9481 aaagttttag aatatctcga aataaaatca tgcaacgagc aggatatgaa caacttaaat
     9541 atgaatttgt ttctgataat atggttcaag gttattttaa agaggaggtt gctaaaatgg
     9601 cagcaaatga cgaacgagtg gcggaatttg tttttgatga aggtaaaagc ttaatgcgaa
     9661 acttaacaca gaaaatgagc aggggagggg tatcaattat taatggctca acaaaagcag
     9721 taactaatca aattaaaaag cagagaacta caggaaaaca gcgctataat caactaaaaa
     9781 agcatggagc gttagaacat attgatgtct caaagactga tttaaagcca cttgcaaatg
     9841 aactaaagaa atacaaggta gattttgcag taaaaagaaa caaagagacg ggtaaaatac
     9901 atgttttctt taaagctcaa gatagagata cactcaattt agcattagaa aatgtgctac
     9961 aaaaatttac taatgaaaaa gaacagaata gagaacaaca gcaggagaaa cctaataaag
    10021 atattgaaaa atcttctgtc gatgacaagg ataaagataa atcgttaact aaaaaacttg
    10081 ataaattaga ggataaagcg caagaaataa atcaacaaag agaacaacta aaagagaaca
    10141 cgaaaaataa aactaagggg caagaacgat gaacatcacc aaaaaagatc ttatttttgg
    10201 tgtcgcactt gctgcttttt tcttttattt aggtacttta ttttttatgg tttatttctc
    10261 gattcctcaa aatacagatt cttcatggat aaatgtagta aatgaagcat ttgataagtt
    10321 cttagaacct aagacattta ccatttctcc atttactttt tcaaaaattg ctatgaaagg
    10381 tggggcgata attgcggtta ttgttttact gattgattta tatattataa gtggcaagaa
    10441 aaatttaaga atgggcgaag aacatgggag tgccaaatgg ggtacttcta aagacattgc
    10501 tccttatgta gataaaaaat ttaaaaataa tcttttattt acaaatacag aaagaataac
    10561 gatcgaaact aagttaaaaa agaataagga atatgaacgt aataataacg ttgtagttgt
    10621 aggtggagct ggttcaggta aaactagatt ttatgtattg cccaatttat tacaaatgca
    10681 ctcaagctat gttattaatg actccaaagg tggaatcttg agtgatacag ctgccattat
    10741 gcaacaaaat gggtatcgga taaaagtatt taatctgaat accatgaaaa attctgatgg
    10801 ttataatccc ttccattata ttagaagaac agaaggagat tgtgaaattg atatattgaa
    10861 agtagttaac aatctaatga agaatacttc agaccctaac aaaaagggtg gagattcatt
    10921 ttgggacgat gcacaaagat cttttttggc agctattttc catttgttga ttttgaaagg
    10981 aaaacctgaa gaacaaaatt tagggatagt agcagactta gtaagacgcg caagagtaaa
    11041 agaagatgat gaagaatata tttcttcgat tgatttagta tttcaagaat tagagcaaga
    11101 acagcctagt tgttttgcag caaaacagta tcagaatttt aagatggcag caggaaagac
    11161 ggctaagagt attttaatta gtttaggtgt tcgtttgtct ccttttgacg ttccagatat
    11221 cagaaaatta acgtcaacgg acaccttaga attagataca ataggagatc gtaaaactgt
    11281 actttatatt attttgccag atactcatac tactatgaac tttctagcaa gtatgattta
    11341 ccaacaactt tttgatacgt tagtttataa agcagataat gtttataatg gatcgttacc
    11401 aattcctgta aggtgtttgt tagacgagtt tgctaatact ggacaaattc cagattttga
    11461 aaatgttatt gcaacaacac gtagtagagg aatttcagtt gatgttatct tgcaaaattt
    11521 aacacagatt agtaaaaagc tttataaaga cagttgggaa acaatcatag gaaactgtga
    11581 ttcttttttg tatttaggag gaaacgagca aagtactcat aagtatattt caactcaatt
    11641 aggaaaagaa acaattgatg tagtaactta taacgaatca aggggaacta cgggaagttt
    11701 tactaaaaac tctcaaaagc agggaagaaa cctacttgat ccaaatgaag tgcgtgagat
    11761 taaaggcgga aaatgtattt atatgttacg tggcacaaag ccttttttat ctgatcgttt
    11821 taagttggaa cgccaccctt tatttaaaaa gttaaaagag acaccttcag gttatatgta
    11881 tcaatcaccg ttggatcaag aagaatttat tgcaaataat gatccaattg attttgaatt
    11941 atcaaaaaat gtcgatatga aggagttttt gaaacatacg gaaatcgaaa cttatgattt
    12001 tgaagacgaa gaaattgaaa caataaattt agatgaatta taaaaggaga gaataattaa
    12061 tgggaacatt taacgctatt atagatattt tgtccaaata cgctattgta ggcggaggct
    12121 tatatgcagt atggggtgca gttgttttag gtggtgcatt aaaagaccac aatggtccac
    12181 aaatgcaatc aggcatttgg caaatcgttg gtggagcttt aattgtagca gcagcggcgt
    12241 tatttaagac aattgtttag aggatagtct ttaatgaaaa ggggatgaat gatggacgga
    12301 tttttaacat ggtgtgtcga atctatttta gaagtaatct cctttagtag taagacaagc
    12361 cacttaacta gcaacttaga aaactttagt cctcagttat acaaatattc aacaactatt
    12421 cttgattctg tctcaaagcc aatagctttc agcatattgg ctttgttttt tgctttagaa
    12481 ttacaaagaa tttctaatcg tgtggagact aatggtggag gtcaaactgg ttttgaaatg
    12541 atttttaaat ccttagtgcg actaggtcta tgtaaactag caatggatca agtatcttta
    12601 attatgcgag cgattatgga tattacagga tatatgactg ataagattaa tcatttaggt
    12661 atgggaaaca aggaagtaag cgatatgctg gacgcaaaaa aaatattagc tcccatttta
    12721 aaagaaggac taatgtttaa agtaggtgta tcgactgttt taattatcgc attactaatt
    12781 actattggtt cgatagtaat cgtacaggta gttgtaaata tgcgttttat tgaactatac
    12841 gcattcttag ctatgtctcc aattccaatt gcgacatttc ctaatgatga atggtcgagt
    12901 ataggtaaaa actttctaaa atcatttaca gcaacagcgt tacagggaac tttaatattt
    12961 atggttatgc agttttatcc atatctctta agttcggctt tcagtgatat aacaaatagt
    13021 ggttcagcaa gtgaaacatt cttaatgaca ctgattggga taattggtaa tagtgtatta
    13081 ctaattttcg cagtaatggc gactggaaga tggtctaaat ccattacaaa tgcaatgtag
    13141 gaaggtcttt tcaatgaaaa aatctcgttg taacagcact ctaattcttg gagtgctatt
    13201 tgtttttttg caaatactat ccatattatt aacaacttat gatatttcaa atccattaac
    13261 actaagtata ctttatgtat tttttattgt ttctcttgtt tttacactca taatgatcgt
    13321 aattattaca aaagagatgt tgcgagattt taagaaatta atatctgata ttttttcaca
    13381 gaagaaagaa aagtaggtgt ttgagtggca attagagtaa aagtaccaaa agaaattaag
    13441 gattatcaag aaaaattaat gggaggaatg agcgttaggc agttagtatt ttttgtattg
    13501 gcagtattaa tctcagtagg agtaacagca tattgtgtat tggtattgca tatgtcggtt
    13561 gacgcagccg gttatatagt tatcccacta ttgataccta ttgtagcatt tggctggata
    13621 agaaaagaag gtatgccttt agaaaaatat ggcaagatta ttctaaatta ttactcgaaa
    13681 actggaataa gaatatatca gactaaagaa aggagcaatc taaatgattt tagaaaaaaa
    13741 cgattccaaa aagtcaaaga aacaaccaaa agataagcaa gcaaaaaaag tattaaaaat
    13801 cttagcaaag cagaaaaaac agcgtggaaa aaatcaacgt agagacgctc cacctttatc
    13861 tagccaacga tcattaacat ataaagcaat gtatgagaat ggagtgtgtg agcttaaaga
    13921 agggctttat tctcgtacat tcaaattttc agatatcaat tatgcaattg cagatcaatc
    13981 tgagcaagaa ttaactttta gctcctattg tgagttacta aatagttgtg attctgatac
    14041 ttctttgcaa atttcgatta ttaatagaag taaagacaag aaacaatttg aaaaagactt
    14101 atcttacgaa ttagttgatg atggactaga tgtatatcga aaagaaatga atcatatgat
    14161 atctaataag attaaatatg ggaatagtag tttacaaaaa gaaaaatata ttacttttaa
    14221 tcaaagagca aaaactttag aacaagctat accagcttta aatagacttc aaggcgattt
    14281 agaagatcaa tttagcgaaa tggggtgtga agtagaagaa ctgtcaggaa ttgagcgtct
    14341 aagggttgtc aatgatattc tacgtcccaa taaaaaactt cattttgaat atgaggattt
    14401 acttttcagt ggcttaacta cacattcagc aattgcgcca gatagttttg attttaaata
    14461 tcgtaaggat cgtttcaaga tgggggatta ttacggtcaa gttttgtatc ttcgtaatta
    14521 tcctgctgaa ctttcggata aattaattac taatatcaca gatatcccta ctaatttaca
    14581 gattactttg catattgagc ctttagatca acaaaaggga ttagatttag taaaaacaaa
    14641 attatcattt atggagcaac aaaagattga tgaacagaaa aaagcggcta gaagtaatta
    14701 tgatcctaac ttgataccaa tggaattaag aagaagtatg gacgaagcac aagaattgtt
    14761 ggatcaaatg caaggagaca atgaaaagct cttttctttt acttttttag tgatggcggt
    14821 tgataaagat gaaagaaagc ttgatgacac aattgagcaa attttaactg caggtcggaa
    14881 aaatgactgc gagtttgcaa agttaaatta tcttcaagaa gaaggtctaa actctacgct
    14941 tttatgggga aacaattttg tccctataca tcgaacatta acaactgcta gtactgctgt
    15001 atttattcct tttactgcac aagaacttat gcagccaaat gggaaatact acggtttaaa
    15061 ctcagtaaca aaaaacttag tgacgttaga taggagaaaa ctaaaagctc ctaatggttt
    15121 tattttaggt actccgggta gtggaaaatc ttttgctact aaaaaagaaa tgacagctgt
    15181 tttactgagt gatcccgatg ctgaagtaat agtaattgat cctgaaagag aatatacacc
    15241 tttagcagaa gctttaaatg gagaggttat tcagatatcc gcaggatcaa aaaatcatgt
    15301 gaatccgatg gaaattaata atgactatgc agatgatgat gatccagtca ttttaaagac
    15361 tgactttgtg ttatctcttt gcgaaatgtt ggtagggggg aatattggtc ttagtccgac
    15421 tcaaaaaact ttaattgatc gttcatgccg atttgcctac aagaaatatt ttgaaaatgg
    15481 gtctggaatg cctactttaa aagatttttc taatattctt aaggatcagc ctgaagatga
    15541 agcaagacaa ttagcagtag agcttgaact ttatactgta ggatcattat cagtattttc
    15601 ccaccaaacc aatgttgata cgagcaaacg ttttatcgta tttgatgtaa aagatttagg
    15661 aaaacaattg cgatcgatgg gcatgttgat tattctagat caaatttgga atcgtatcac
    15721 aagtaatcga aatgcaggac gtcggacatg ggtatatatt gacgaaattc agcttctttt
    15781 ttcaaatgaa tatgcagaga attacttttt tgaattatgg tcaagagcaa gaaaatgggg
    15841 agcaattcct acaggtatca cacagaatgt atctacgctc ctatcttcat taaatgcaga
    15901 acgtatgcta tcaaatagtg attttgttct aatgttaaat caatcccaac gtgatcgaga
    15961 aagactaggc gaattgttac acatttctag ccgtcagaaa aaatatatta cgaatgccaa
    16021 agaaggtgca ggattgatgt atgcgggcga ttctatgatt ccactttatg atgagtttcc
    16081 aaaagatact aacttatata aagtgatgtc aactaagatt gaggacgtgt ataagtatca
    16141 aaatgagcaa agagaacaag gtactacagt atgaacacag attttcgaga acggattatc
    16201 cgaaaacaaa agacagcaaa aaagtttgta gtaaatcgag ataataatca gttcaagaaa
    16261 aaaattgtat ctactgacta tacaaaacga gctaagaaag ctaatcatag tggaaaaata
    16321 aaaagtagtc ggggcacttc ctatttaata aaaaaagatt cagcaaaaaa tgtagtcaaa
    16381 gatgttaact ctaaagcaca aaaaaatggt gttgaacagg ggaatgcaac tgctgaatta
    16441 gaaacgagta aaacaatagg acgtgggata aaaaatagca ctaaggcaac aaaaacagtg
    16501 gctaaaacta caactaaagt tactaaaaaa agtaaagaat tgattcaaac aaaggcttca
    16561 aaaaaagaag ttttaactgt tgttaaaaaa ggaagtaaaa aaggtctcgt aaacacaagc
    16621 aaagctgcaa aagatacagt aataaatgaa atccaagagt ttagaggtga ttcagaatct
    16681 caagggatta atgaagcgat tcaattaaaa gatttcattg atacaggtat cagtggtgct
    16741 aaaaaaacaa aaaaagcagc aaatactgct aaaaaaagta gtaaagcact aacaaaattt
    16801 ggtaaatcaa gttacaaata tacacaaaga attgctgaaa taggcatacg tagagttcaa
    16861 caagttttat caaaagaagt agcaaaagca ctcattgcta aattagcgat tccactaact
    16921 ttagcagtgc ttatattatc aattgttacg gctgtaattt caactgttaa catttttaat
    16981 ttcggagcag gcgactgtga tactagtgcc aactatgaca atataactac caaagatata
    17041 gagtcaaatg ctaaaagtat ttactcaatt attcgtaaag aaatacctga gtccacccct
    17101 caagggattt ctggagcatt aggagctatg caatttgaat ctcaattgaa tccaaatgca
    17161 gttaatccaa gctcaggtgc gacaggaatt gcccagtggt taggttctcg tttaacctct
    17221 ttaaaagaat ttgcacacaa aaaaggaaag aaggaaacag atttaggtgt tcaagtagaa
    17281 tttctgttat ctgaattaaa tagttcatat tatcaatcta gcaaaaaaat attagcaatg
    17341 acagatgttc atgaagcatg caaagagtgg gtcatgaagt ttgaaggatt atctcaagac
    17401 tctagccaat ggtatttgga tcaaagaaac tcttatgctg atcattggta cgcaacttta
    17461 gggacgagtg atccaattgc tgaaggtact attagtaatg gtgcagatag cgaattaaat
    17521 acgcttactt gtgatttaga atctgatggt ggcgatatta tggaaattgc taagagttgg
    17581 ctaggctggt ttcactatga tcagatacac cctgctcctg atttaggaac agatttaaaa
    17641 aatcctaata aaaatggaag aactgattgc tctggttttg tttggctagt tttaaataaa
    17701 gctgggtata aagtacctgc aaatatgcaa tggtttactg gatcaatggc aagtgacgct
    17761 agaggatcac accaatattt gaagcaaatt tcagagacag aagcaaaagc aggcgatatt
    17821 gttattgtta atcaaggtgc tggagcaggt agtaatggac acacaggaat tttgactgaa
    17881 aagtggagag gtaaagatac caaaatcatt caagaaggtg gaaatggcga tagtgtaaat
    17941 attgaagctt ttggtacgtc ttttatgagc ttattagatg gtggcgatgt tgtattagca
    18001 cgtcctataa aaaaataatt agaggggagg aagtactttg ctcaacagaa aatcagctac
    18061 tataactata tttgtattga tcgtatcagt attttcttta tccattggat taaatattaa
    18121 gcaagagtta actgtaaaaa ctgcaaattc taaaatagct gctttagaaa agaaaaacga
    18181 tattttaaaa agagaaacga aagaccaaaa gagccgaatt agtaaacttg aagatgaaaa
    18241 taataaaatg attgaaacac aaatagtcaa taaagatggt gatgtctaca aagaatttac
    18301 aaacattgct actaaatact ttaaatcaag atttaattat gatcccaaaa catacaaaga
    18361 aagtaaggaa aacgtaagag atttgatttc tgaagagttg tataagaaat tttcggagaa
    18421 tcagctaact tatggagatt ctaataatgt gagttctcga ttagataatt tagaaatata
    18481 tagtggttca ttaattaacc aaaaaactat tactggtctt ttgattgttg actatgaaag
    18541 taaattagga gaaactgact ggtttaaaaa ttcagaaatc tatactgttc agtatgatat
    18601 tcatgcgaag aaattgacta aaatacaaag cttggggtca gttttaaggg gagatatgat
    18661 tgaataaaaa atttttttct atttgtgtct taatttttac tgtgtctatc atatttttag
    18721 cagatcattc ttatatcaaa tatatagaag cacaaaatga tccagtctat ttagatgaat
    18781 caaatcaaga gaaaatagtt ttctacagaa acgattgtag aaactgtgaa aagatcatgc
    18841 cttacctgat acgtcacaat tttttatacc atgacttaac ttttgttaac atgaataaca
    18901 aagaaaataa acgttatatt aaggagtatg gtttgacttc cgttcctaca atcatttccc
    18961 ttaagcagcg ttatgtaggt acagatataa gtaaaataaa aacattagtt atgtcaaaag
    19021 gagatgattt ctgatggcaa gaaagagctt aacagaacgt attcagcaga agaaagaaga
    19081 agcaacaaag cttgaaaaga ggattcaaga agatcaatat aagctagaaa aactaacaaa
    19141 gcaaattaga gatttagaag cagaacaggt tttagcgatt cttgataacc ataagcttag
    19201 tgtgaaagat ttgaataaaa tactaagtaa acagcctgat aacaataatt ttatcaacca
    19261 taattaatta aatatatatc tttttaattg agataataag cgatataata agataaacaa
    19321 gtaataagag ggtgaaagta tgttaatagc taaaagaata ttagcaagta tagtagcaat
    19381 tccttgcttt ttactttcca taattgttat tgccgttaac ataatttcca tacctatttt
    19441 gcttgttata cagattctta aaaaaattgt gatccttttt attacattag tatggattgg
    19501 ttttataggt ctgtttattt atggattaag ttctttaggc ttctctgaaa cgcttagtat
    19561 gtttacagtg cctactatag gtatattaat ccttacatta atactatcta tttttacttt
    19621 tatctcagat aaaattcctg attttcttgg aattgttagt tcatttttcc tttctatccc
    19681 aaaatttcta tggggggctg aagaaactga agaataaaac attcaagggg gaaaaatatg
    19741 tttttaaaga aaaaatatgt tcggtttata gaacaaagag atattgaatt gccagaaacc
    19801 cctgtagaaa tttacagggg tatttattta cttgaacagg aaaacgatcg gctaacagac
    19861 aagcatacta aaggttctac attaatatta ttaaccgata aaaagggaaa tgaactaaat
    19921 agacgactaa tccaattacc aatagaacaa ggtatagaga ctattttgac taacctttta
    19981 attgaaccta cctcaaataa aaagcaaagt tcttctaagt cacaaatgat attgaatgat
    20041 atagaagaac caaaaataaa gaataaaaag aagaccgtaa attatcagac ggtcttcttt
    20101 ttatttacta ctaatttagc aaaaaaaatc tacatggctt tgataatagt tctgttccta
    20161 tttttcacaa gtttttttgt attaaataat cattcatcaa cttctactga ttctcaaagc
    20221 tatcatgagc tgttaaagaa gaaagattat gaaaaaataa taaaacaata tcctgaaaaa
    20281 gaaaacgaat tagtagaaaa actgtatagc gaaaaaaatt ctaaggaatt aaaagaattg
    20341 gcagagaaat ctgattcaca gatagctctt ttttattggg atttcttgaa tgagcattgg
    20401 caagcagtaa ctacgatgaa aaatgttaca ccaaacgaaa caatacaagc tatgcgtggt
    20461 tatgcatact taaaacaagg aaaactagac gaagcagaac taattaacaa agaaattgat
    20521 aatcaaacat taaaagatca aatttatcaa tttaaaaagc aagaagcata taaagctctg
    20581 catgaaaaag atatcgagaa ggcagagaaa ataaataaag aaataaatga ttctgaacta
    20641 tccgaagata ttaaagtagc taaaagtatg gttaatctac ttcaaaaata tgaaaaggac
    20701 agatcggatt caaaattatc agaagatgaa agaaaagaag ctgagaacaa ttacaaaatg
    20761 tggtcagaaa acttaaaaca actaggagga aacgataatg cataacaaaa agaaattttt
    20821 agtagtggga ctattttcag ctgttttatt agggggtgca gttacttatg gcatgtattc
    20881 tcaacaacca cacaatgaga ctgaaaagac agaacaaaca actaaatcag agtccagcaa
    20941 acacaaaagt aagaaatcag catgggaaga aagtgtgaaa ggcaaagaag acaaaaagga
    21001 caaaaacaaa cattctacaa agaaagctga tgatatattg gctgttattg ctgatacacc
    21061 gttggttaaa caacaaccta atcaaaacaa aaataatcta attgatagat tagcagtagc
    21121 tttagatgat cagactaaaa aagaggcaga cagacaagaa aaattaatta ttaaagataa
    21181 aaaatctgta gatgagccag tactagttga tttgagttat aaacctcaat ttgaggatac
    21241 taaaccttca aataaagaga acggtaaaac agaaaataat aaagatgaaa atagcgaatc
    21301 agataaagga aatgatacta aacctgataa ttcattacct gatccattgc ctgtaccaga
    21361 gccaaatcca ttgcctgatc caacacctag tccagaccca aatccaatac caacaccaga
    21421 tccagaccct gatccagatc ctgatccaac accagatcca gaaccttcaa ttgatgaaca
    21481 aattaatcaa tctaaggaag aactcaatga ttctaggaaa caggctaatg aattaaataa
    21541 acaactaact caagtaaaag atagcttgga taacttacaa agtattggag caagtactga
    21601 aaagaacgtg gataaatcag cattacaatg gcaaaaagtt gctgacttag tcaatgaata
    21661 taatcaactt acaaatgaga tcaagaaatt aattgaagaa gacggaacag tgttgccaat
    21721 caattatgat ctttataaag aaacgtatga aaaactaaac caaaaagtca ctgaagtaaa
    21781 aactgcacaa aaagaagcaa ctaatacgac aaatgaaata aataaaaatg tagaacaagc
    21841 aactgatacg ctaaataatt tagaagaaac taaaaaagaa tatcaaaata aacagaaaga
    21901 tgtttctgag gtggattcta acgtcaattc ggctattaac catgcacaat ctaacgaaca
    21961 agttgcttca gctgttcaag atgagataaa tcaggctgca caatctaatg agcaactaaa
    22021 tgaaactaat aagactgttg gagaacagtt gaatcaagtt gatccaattg aaagcgaaac
    22081 tgatattgct agcgctaagg agcaaactgc tgatatcaat aatcaagctg aagaacaaaa
    22141 taataatgtt gacgatatga tgaatgattt taacaatttg ccaaaaccga gtgatagtga
    22201 agaacctagc gttccagaaa atacagatca aaatcaagat tcaacaagcc aaccacaaaa
    22261 aatagataat gctagtgttt ctcaaaacaa attagaaaat gaacaggtta gcgaaaaaac
    22321 attaccagtt actaatcaaa atagtaacca atccgaaatt gaagatattc aaaaataaaa
    22381 gtgaggaatg aaataaatgt cttataaaga tagtgaagca aaaaaaaata ataaaaccgc
    22441 tgaattactt tcacaggcag aagaaggtat taaagaagta ttatcttcag acaaatacaa
    22501 aaattttctt cgtgttatgt ctaagtttca taattacagc ttaaataata cccttttaat
    22561 tcttatgcaa aatcctaatg ctactcatgt tgctggttac aaaacatgga aaaactcttt
    22621 taatagacaa gtaaataaag gagaaaaatc cattaaaata attggcggtc gtccctataa
    22681 gaggaaagta gaagaaataa agaatgggca aaaagaagaa aaagaagttg aaggtatttc
    22741 atttttccct gtttcagttt ttgatgtttc tcaaacttca ggagaacctt taccgcaact
    22801 aatgaatgaa ttaagtggag aagtaaaaaa ttatccagag ctttatgttg ccttgaagca
    22861 aagtaccaat ttttctatag aatttgcaga tatacaaggt ggcacaaaag gatattgttc
    22921 tccattagaa aagaaaattg tattaaataa tggtatgagt caagcgcaaa caataaaaac
    22981 tctgattcat gagatcactc atgctgattt acatgcacca gaatttgata aaaattcgtc
    23041 aataaaaact acaaaatcta caaaagaagt tgaagctgaa agtacggctt ttgttgtttg
    23101 tgaacattat ggaatagata caaaagacta tagctttcca tatttagcag cttggtctag
    23161 tgataaagag ttaaaggaat tgaaaaatag ctttgaagtc attttaaagc aagcagataa
    23221 tttaattaaa aaaatagaca agaatttagc agaattacaa aaagaagtga caaaagaaaa
    23281 agaagaaaaa cagagtactt tgtcattatc tgatcaatta gaagaatttg aagataaagc
    23341 aaaatcttta aatgaacaac gtgacaaaga aaaattaatt aataaaattc gccaaacaaa
    23401 agagcaaaaa gacgtttcta cactatagtt taaagtagat gaaacgatct attttgcttt
    23461 tttaatttat cagaaaaagt aacctttata ataaatgagg ga
//
LOCUS       DACEMO010000023        21863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEMO010000023 DACEMO010000000
VERSION     DACEMO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.023"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..830)
                     /locus_tag="IW728_000694"
     CDS             complement(<1..830)
                     /locus_tag="IW728_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1024074.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     "
     gene            complement(1020..1322)
                     /locus_tag="IW728_000695"
     CDS             complement(1020..1322)
                     /locus_tag="IW728_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1024075.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(1348..1779)
                     /gene="ruvX"
                     /locus_tag="IW728_000696"
     CDS             complement(1348..1779)
                     /gene="ruvX"
                     /locus_tag="IW728_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1024076.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(1776..2042)
                     /locus_tag="IW728_000697"
     CDS             complement(1776..2042)
                     /locus_tag="IW728_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1024077.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(2296..3021)
                     /locus_tag="IW728_000698"
     CDS             complement(2296..3021)
                     /locus_tag="IW728_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1024078.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(3387..3457)
                     /locus_tag="IW728_000699"
     tRNA            complement(3387..3457)
                     /locus_tag="IW728_000699"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3423..3425),aa:Gly,seq:ccc)
     gene            3574..4326
                     /locus_tag="IW728_000700"
     CDS             3574..4326
                     /locus_tag="IW728_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1024079.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4342..4821
                     /locus_tag="IW728_000701"
     CDS             4342..4821
                     /locus_tag="IW728_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1024080.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(5072..6652)
                     /locus_tag="IW728_000702"
     CDS             complement(5072..6652)
                     /locus_tag="IW728_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1024081.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(6645..7388)
                     /locus_tag="IW728_000703"
     CDS             complement(6645..7388)
                     /locus_tag="IW728_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024082.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(7564..8340)
                     /locus_tag="IW728_000704"
     CDS             complement(7564..8340)
                     /locus_tag="IW728_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1024083.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(8588..9424)
                     /locus_tag="IW728_000705"
     CDS             complement(8588..9424)
                     /locus_tag="IW728_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1024084.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(9435..10364)
                     /locus_tag="IW728_000706"
     CDS             complement(9435..10364)
                     /locus_tag="IW728_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1024085.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            10556..10909
                     /locus_tag="IW728_000707"
     CDS             10556..10909
                     /locus_tag="IW728_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1024086.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(10983..11639)
                     /locus_tag="IW728_000708"
     CDS             complement(10983..11639)
                     /locus_tag="IW728_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1024087.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            11865..13799
                     /locus_tag="IW728_000709"
     CDS             11865..13799
                     /locus_tag="IW728_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1024088.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(13885..15108)
                     /locus_tag="IW728_000710"
     CDS             complement(13885..15108)
                     /locus_tag="IW728_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1024089.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(15102..17000)
                     /locus_tag="IW728_000711"
     CDS             complement(15102..17000)
                     /locus_tag="IW728_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1024090.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(16994..18112)
                     /locus_tag="IW728_000712"
     CDS             complement(16994..18112)
                     /locus_tag="IW728_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1024091.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(18195..19028)
                     /locus_tag="IW728_000713"
     CDS             complement(18195..19028)
                     /locus_tag="IW728_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1024092.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(19021..19938)
                     /locus_tag="IW728_000714"
     CDS             complement(19021..19938)
                     /locus_tag="IW728_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1024093.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19961..20140)
                     /locus_tag="IW728_000715"
     CDS             complement(19961..20140)
                     /locus_tag="IW728_000715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024094.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(20165..21289)
                     /locus_tag="IW728_000716"
     CDS             complement(20165..21289)
                     /locus_tag="IW728_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1024095.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            21472..>21863
                     /locus_tag="IW728_000717"
     CDS             21472..>21863
                     /locus_tag="IW728_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1024096.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPA"
BASE COUNT         6816 a         4377 c         3607 g         7063 t
ORIGIN      
        1 aaaaaagact tttcagtgat tttgacttgc ttgaatccag atccttctaa tgtttgcaag
       61 atgacgaaag cattgtaaag attgatcaaa agaaccacga tgacaatcgc ttgaaaaata
      121 aaggtcactt tttttggaga caaaaccctg ctaaatcttg ctccccacaa acctccaata
      181 acagcagcgg ggataacata aaacaacata gataaatcaa agcgcataaa gctacttgtt
      241 aaagcaatcg ttaccaattt tgccagttga gaaaagaaaa tcgtagatag agaatatagt
      301 gttgcttctt ttattggcaa tgcaaacatc agcatcaaca aagaaacatt gactggtcca
      361 ccaccaatac ctaaaaaact agctaaaaag cccagaaccc ccccacatat aaggtaccaa
      421 gtccatgacg tcaaatgaaa ttttggctga tgatgcttgg tgtagaagaa cgcaaaaacc
      481 aaggtcaata ccgtcaaaaa aatttggatc agctgcacat gcttctcgtt ttcaaataac
      541 tgtaaaaaaa cttcgaatac aatgttgccg actactcctc caacaacagc tccactagat
      601 acccaaccga caatctgcca attcagtgat ttcccgcttg atacttgtcg cacagtcgaa
      661 acaatggaca tcgtgaatac cgcaactgtt gagtaaaaag aaatccctgc aacagaatgg
      721 gcgccgataa gatcaagaat cggtttgatc aacacgcctc cacccatccc ggaaatagcg
      781 ccaactgtgt tcgccagaac gatcaccaca aaataaatca gaccaatcat cttgtatttc
      841 ctttctcttg tttatatctt ttagcgattg ttactctttt cttttattat acagtaaaag
      901 aaacaaacga ggcttcgaaa gcttttctac tttttaagga aatttagtgc taaacgaaaa
      961 aaccgggctc tacttttcag agagtccagc ttttcttaca ccttatcgat tcttttgttc
     1021 tattcttctt ccgccatgaa tgtattgaat acttcttcga tcatatccca ttctgcgtct
     1081 gtttcgattt gttctaactc gccttctgtt ccatcttcgt tttcgatgta tgcataagct
     1141 tgtaattcta catcttcatc ttctgaaaca ccagctggat ataaaagaac atagttacgt
     1201 ccaaattctt cttgtccgtc aactgttagc aagatttcgt ataatgtttc atttccttgg
     1261 tcatccacca atgtgatgtg ttcgtgtcct tcgtgttcgt gggaatggtt atgttctgtc
     1321 ataagattac ctcttttctt tttattatta aaataattgc ttacctgagc cgtctaaata
     1381 attttgtaaa atcatcacag ctgctaattt atcgatgact ttttttcgtt tcgctcttga
     1441 agtatttgcc tgctctacta gcatacgttc tgcctgaaca gtggttagtc gttcatcttg
     1501 ataatcaacc ggcaaggaga atagttcttt gatcttctct ccatatgaca tggaagcttc
     1561 tgcccttggt ccgatggaat tgttcatatt cttaggtaat ccaactacaa aacggtctac
     1621 ttcgtactgc gccactaatt ccttcaaccg atcaaaacca aactcttctt ctgcctcatt
     1681 gattcgaatg atttctactc cttgagctgt ccagccaaag ggatcgctca cagcaacgcc
     1741 tactgtctta gaaccgacat ccaagcccat tactctcata aattcactcc atgatttgag
     1801 aggtagtctt tgacaattac ttccatgatt tcatcgcgtt cgtgacggcg aatcaaattt
     1861 cgtgcatcac gataacgagg aatgtaggcc gggtctccag aaagcaagta gccgacaatc
     1921 tgattgattg gattgtagcc tttttcttcc aaagcccgat aaacaatcgc taatgtttcg
     1981 ctcacttctt ttttgcgact atcgtcaaaa ccaaaacgta cggtttcatc tgtaaaaccc
     2041 atgattctac acctctttcc cgcaaggtaa ttccagctta ccttctacac ccttatttta
     2101 ctcgaagaaa gggaaaacta caaacaaata ttccaaactt ctgaataaat taagaaacac
     2161 gatttgtctg atttttcctt ttttctgtgg ctttttcaaa agccctcttt ccataaagta
     2221 tacgctatat ttctttgaaa gtatgttttt acacaatttt ttcataaagt tcataaaaaa
     2281 ttcgaattgc tgctactaat ttttcgtttt cgttgcttgc cctgtctcat aattgatggt
     2341 gtagccatct tgaacattgt agataaagac attgaattca atttttttgt cttcaatgct
     2401 ttctgcttcc aactgcacac ctcttgctac cagttcattt ccctcgaaat gaggagtgac
     2461 acgatagcgt acatgatgat ttgtttcctt gatatatgcc gcaatatcat tttcgtgggc
     2521 aagcatgtac ggagcattca acgaacgtgt ccctgtcatt aagtttttaa tgttattatt
     2581 ttgaccagtc aactggaaac caatcaaatg gctgcggttg tataaccatt gtccgtcact
     2641 taatttcttt tgtttccaac ctgtcggatc aatatacaaa ggttctctct tttcagtagg
     2701 aaacaagtct tttcccaaca tcgcattggc ttgtcctact ctgttcaatt tgtctatatt
     2761 tgaatagctt tcccatgtcc ctttggaaag actaagatcc tcttttgtaa actccggctg
     2821 attcccattg atttctattt cttgatgttt tccatcatat tctggcacat ccgctacttg
     2881 agaagcttgc ggtgtggaat caaaaatggt tgtaatttgt tggaagatat ctgaatcttt
     2941 ttgtgtggtg gaaataccga gtgcccctgc aatcacagca gcgacgatcc ccacgatcgc
     3001 cttggttgtt tttttgctca tttctttact cctttactaa cagttcccta aatcttaaca
     3061 tatgttcccc tataaataaa tactggaaag aaatagaaac agtaaaaaaa taaaaccagt
     3121 ttttagacgg cttaagaata caaaaaaaga gacggattca tcgtctctac tcaatcacaa
     3181 aacactattt acaaggtatt tattatcatt caaaaaattt ttcttaatac tcaaaactaa
     3241 acggcttctg tacgacctct tggtaaacag tgttcaaaag cttgcatctg cggtaataag
     3301 tccaaccaag tcaaaaacat aagaagcttt tttgcttgac tattcttatt acttgatgca
     3361 gggcagcttt ttcacagcct cttttaagcg ggtgacggga atcgaacccg cgacatcagc
     3421 ttgggaagct gggattttac cactaaacta cacccgcgat acaaaaacag tatacgcctc
     3481 tttcactcct tcctcaagtt tttttattct ttacgcttcc gatatgtttt gtagtaaaat
     3541 aaaactaatc aatcaattat tggagaagat gctatgacgg gaaaagaatt agatctgttg
     3601 ctggacactt gtttactcgc tgggaaaatc atgatggaaa gcaatgcgga gatgtatcgg
     3661 gtagaagata cgatgagtcg aattgcttta gcttctggaa atttccgttt ggtcagctat
     3721 gtcactcaaa ccggtctctt tattggtctt gatcgtacct cgaccatccg aatggaacaa
     3781 attaccaacc gttcgattaa tttagaaaaa gtagtaaaag taaatgatct ttcacgtaaa
     3841 tatgttgctt cagagctaac attagaagaa ctttactgcg aactgcaagc aatcgaccga
     3901 gaccgttctt tctttcctgt ctggttgcaa atcgttagcg cggcagtgat cagtggaaca
     3961 attatggtgt tatttggcgg aaagatgacc gatttacctg tgactttagt cattggagga
     4021 ttgggatacg cagcttatct atatagtttg aaatttttta gaatcaaatt tttatcggaa
     4081 tttttatctt ctttattgat tggcatagcc gctatctaca gcgtacgttt tggcttagga
     4141 agcaatcagg atctaatcat tatcggatgt gtgatgcctt tagttcctgg agttcaaatt
     4201 acgaacgcta tccgtgattt gctagctgga cattatgtct caggagtttc tcgcggaaca
     4261 gaagcgatga tgactgctac gatgatcggt tttgcgattg cttttatttt ccagcttttt
     4321 tattaggaaa ggaacacaag tatggggacg atatttattc agttttcttt tagtttttta
     4381 gcgactgcgg catttgccgt tattacaaat gtacctaggc gttcattgat ttgttgtggt
     4441 ctttctggtg cttttggctg gatgatctac tggatctgtg ttcaaatagg cggatctgct
     4501 gcttttggtt cattgcttgg ttctttagga gtagcattca tcagcgatct tttctctaaa
     4561 cgtttgaaaa tgcccgtaac gattttcaat attcccggga tggttcctct agtccctggt
     4621 ggtttagctt atcaagcggt tcgaaatctc gttactgaat cctatcgaga agcggctcat
     4681 tatgcggtcc aagcaattat gattgctgga gcaatcgctt taggtcttgt attatcagaa
     4741 gtctttaacc ataacatccg taatttcaaa gtaaagcgtg acgcaatcat gatgaaacga
     4801 aaaaaacggc tttttaaata aacgaagtgt aacaaagaaa aggttgtgaa agagacgttc
     4861 agccttgagc gatgacaaaa tatctcacaa acattgcgac aagtaaccgc aggagcaata
     4921 agaagtaatt atgagtatta aaaaaagttt tgtaaaaata gcttctcaca tttttacata
     4981 aaattgactt aatactgaga aactgacttt tgaacaccct ctatctgtaa aaaaggggct
     5041 gcgacatgat ttttgtcacg gcccctcttc tttattcaaa ttttttccaa aacgttcgtt
     5101 gataataaac gatccataga gcggaaagta ttagaactaa accgatcaca ggaatattca
     5161 ctaaccaaaa tggataaaac aaagccaaca ttccgtataa gacgataaga ataatactga
     5221 tgatcaatga acccatcata gttgcgacta aacccaaact tcctgaacct ctgttaaata
     5281 attggttaat attggtccag ttcagcagca gcaaccgata atctctagcg aaataacgta
     5341 gtgacaataa gtagcagccg aataaagaac caaatagcaa gctagggata aaaaacaaag
     5401 gcagctgaaa agacagtccg cttaataatg cgatcccacc agttaaaaaa ctctgcaata
     5461 tccatccgaa tctgaacttt tcttttaaat attggttcat agagattggc aaagaacgga
     5521 taaacagaaa attttcttga tccaaagaaa taagattgct gataaaagaa gtctgattaa
     5581 ctgttaagaa agccaatgcg atccctacca ggaaaacaac accgattaac ttattatcta
     5641 aatcagttaa ttttatttcg cctcctaggg caaaagcaag aataaaaatg attggagtta
     5701 acaaggaaga agaaagaact tgcataatca aattaggttc ttttactaat tgagcattgt
     5761 aactaaataa aagctgatgc acattttggt tctttttatg ggttcttttt attttgaact
     5821 cactgtttga atcagtcagt tgctctgtaa gctttggtac tatcagctgt tttacagcaa
     5881 ggaaaaatcc cagagccgtg atagctagta ttccccaacc caataaacct tgttgagaaa
     5941 gtggctgact ggcagcatca tagaatggca ataatatcga aatcggatgc cgatctgtta
     6001 actcattttc aatagtcccc gtttgatgat tcatccacat gattccagca aatgcgatca
     6061 gcatcgagct gccaagcaag atactagaaa ctgtttttct atgtttttta aacactgctg
     6121 ttcgagccaa accaaaaaca atcaaactag ccagacaaaa aagaataatc aaaaatagag
     6181 aaaacaaaag gattgagata atgatagtaa gcaaaatccc tagatcagct cgccatccgg
     6241 ttaaaataaa catgactaga agcggaaaaa caaaaggaat gatggtcaaa gcaactacca
     6301 aaatctttgc tgaaaaaacc tgttcttgtc caaaaggcag aggcaaaaat acttgcaggt
     6361 catttccttc aaaaaagata ttataaatga ctgatatact ttgagttaaa cctaacatac
     6421 caaaaagtgc cacgtaataa gtaaagaaac ctggcaattt aacaaagttc atagcaaaca
     6481 tagcaaatcc gtaaatcagt aaaaaaatca gccctgaaaa aagattttga gacaaaagat
     6541 agatagttaa tcgctttccc ttttttcctt tctctcttgc tttattcgtg agctgtggat
     6601 taacatataa aagattaact cgaattaatt ctaataattg tcgcttatac atacggtcca
     6661 tctcctgtcg cttgccttcc agccattttc aaataaatct tttctagcgt ttcatcaggt
     6721 gagccattca ataattcact aactgccccc tgataaatca attcaccttt cttcaaaata
     6781 gccagtttat cgcataactg ttgtgcggta tccaatacat gtgtagaaaa aatgactgtt
     6841 tttccttttg cggcatgagc cttcatcatt tctttcaaat caaatgctgc ttgaggatca
     6901 agtccttgca aaggttcatc caatacccaa atatctggat ctggcagcaa cgtgccgatc
     6961 aaaatcgttt tttgacgcat tccatgtgaa aaacttgcga ttgtttcatt ttgatgacta
     7021 tacatatcaa aaagcgctgt caattctgct aaacgctttt cttttttttg tgtgtctaat
     7081 tcataggcag ctgaaatcaa gtcccaatat tctccagccg tcaattgtaa aaaaatatct
     7141 ggggaatcag gcacataacc gattttttgt ttgatactca atcgattttc cgtcaatttc
     7201 attccatcta ctaaaatcgt tcccgatgat ggttcaataa tactaactaa acttttaatc
     7261 gtagttgatt ttccggcacc attatgtcct aaaaatccaa aaatttcccc ttgtcttatc
     7321 gagagagaca agtcttttaa tgcttgtttt ttcccgtaac ttttacttac attgatgaat
     7381 tcaatcattt ctgtactcct tttgtttgtt attacttttt attgtaccct tcgttattgg
     7441 tggagtaaat gtcaaaaaga gtgcataaca aaaaaggata gaaacgacta ggctgctact
     7501 tgggttgttc tcttctacag gaaaacactc ttctaacagg taagatatga gtctttgttt
     7561 tctttaaaat gattcatttt ctatcttttc ttgcataaaa ttctttagtt tatcaatact
     7621 tctctttccc atctctagtt gttctccatt tgcaaatgtt actatttcgt ttactctgct
     7681 aatagatgta atgttttcag tattaatgat ataagctttt aaatctttac ataaatactc
     7741 atattcgagt gattttttat attttttaat tgtgccgttt acgagaatct cttcatcttt
     7801 caatactagg cctattttat ttttataacc tggcatgctc attagataaa ggatttcatc
     7861 gtaatggata tagtgatcgc tattaccata acggacagtt gtccaccttt ttttcgtagc
     7921 atgttgaaca ggatagtttt ctttttcttt caataagtaa ctttggatgc tttttttatt
     7981 tttttcataa gtgaattctt ttatcaaata accactaggc gtaatctttc gattaataac
     8041 agttattgct ttattcggat gagacgttag ataaacaatt ttacactttt cattcttttc
     8101 tctaagcatt tcgcctagtt cgataccgct ataataggcg ttcaaatcga tgtctaggaa
     8161 aaaaatatct gaggaataga tttcattatt tttaatgaat tcaacaaatt gtataacgtt
     8221 caatccctga acgatctcaa atttccaagg acttaataaa attctggatt cttccaaaat
     8281 ttctactagc attttttcaa aaaccaaact atcatccaaa ataaatatac gcgttaccat
     8341 aaaaagtacc tccactcgat attttactat caaaatcatt tcatacaact tttcaacata
     8401 catgttagga ataaattgta attctcttca caatcttttc caacaccttt gaaaccgata
     8461 tcaaatactt tcaggatgct gcttctaaaa taaatatcaa acaaatgaat acatcatgca
     8521 aaaaaagcca gacaaccgac gtccttgcgt gtcagctgtc tgagctatgt ttatttattt
     8581 tgttctttta aaaagaacga tacacatact gatttttcgg agcagagact ttctcagcgt
     8641 ttgtaacaac tacagatggg atttcacgct tgaatggttg ataatagtct gattggatgg
     8701 ttccttctat tttataccat tcatcatttt taaccgttac tccttccggc atatgcgtca
     8761 acaaaccaaa gactcctgaa tctgccacac aatggataat gccgaatcgg aacaagaaga
     8821 tatctgtatt gctatcgttc ggtgtttggt aaacaaatcc ttcatagctg atttttttgc
     8881 caatgaattc gctagggtaa ttataaatta gctccataac ttctaaatag ttttcatctg
     8941 tgatcttgat agtttgctta tccccatact tttctttcaa ctcattcatt tggtctaagt
     9001 agtctgattt gttgaaataa atactggtat ccggctttaa gtattgtgtc tgcatctccg
     9061 gatcccctac tgattcttta cttactggga aattaaatcc tttggcttct acgatcgtag
     9121 tgtcgagact aactgttgga aacaacgtcc ccacgattac tggcaaagct agcagtacat
     9181 aagcgattcc acgttgataa ggttttgaca acccatggtc atgatcgtgc tcgtgtgcct
     9241 tttcttctgc ttttccgttt ttgacccaca aatacagctg aactaaagcc aaaagagcag
     9301 ataaaatcat tgaaagaata gctaagtagc gataatggac attgatatat tgatcgagtt
     9361 tgccagaaac ttgcaagtac atcatcaatt cgaaataccc agataaaatc aaaaaccgca
     9421 gcatattttc cctcctaaat aaataatgtg aatccaataa cgctaagggc aatcagtccg
     9481 atcagcgata aaataaagcg tcctgtaaaa tatcgtttca tcatcagtag atttttgata
     9541 tcgaccattg ggccaaagac caaaaaggca gctacaggtg ctgtgccgaa cagacttaac
     9601 aaagaagatc ctataaacgc atctgcttct gagcaaagag acatcgtaaa ggccaataac
     9661 agcatgatga taatggctaa aatctttgtt gagccgagcg ttagcataat tctagttggt
     9721 aaataagtct gcatcgcagc tgcgattaat ccgccaatga tcaaataacg acctgtatca
     9781 aaaaattcgt cgattgaatg tgtcaacaca tgcccagttt gatggatcag ctgatttttt
     9841 ggttcattct tctcactttg ttcttcttca tggacatgat gatggtgatt ttctgctact
     9901 ttagacgtga ttttttcttt caatacagcg tctttttgaa aataggcaag ccagacacca
     9961 atcacaaggg caacaaaaaa gctgcctatc acccgccagc ttgccatctg ccaagaattg
    10021 ccaaatgcga taaaagtgga aaaaatcacg attggattga tgatcggagc agtcagcata
    10081 aaagcaaacg ctgtgtgaac tggtacgcct tttttaacaa attgatgaac gatcggtacg
    10141 atgccgcatt cacatgaagg aaagaaaaat cctaagatcg aaccagtaaa aatagagaag
    10201 atacgatttt caggaagcca tctcttcaca cgttctgggg tcaaaaatac ttgtaaggcg
    10261 cctgagataa gacagcctaa taatacgaaa ggtaatgctt caataacgat cgataagaaa
    10321 atcgtactca tctgcaaaac cgaatttgga ataaaagaaa acatgagttt cctccttttt
    10381 atttctagtt cattcgcttt gcaaatatac gcttttattc aaaaaaaaca agttgctctg
    10441 taaattttta cacaactctt attttttacc aaagcacttc aattgacttt ttctgttatt
    10501 cccattaaat taaggtctca tacatatcac ttctatcata aaagggagaa taaaaatgaa
    10561 acaaaccact aacttgactg aagttcaaga catactgcaa cagtcatccg ttgccgtcat
    10621 ttatctttcg atgcctaatt gcagtgtttg tcacgctgtt cgtccacgac tagaggaact
    10681 tctcacacat tatgatattc cggcacttca tttggatgca tttgaaacac cagaagtagc
    10741 tagtaaattt gaagtgctga ctgcgccagt tgtcctgatt tttcatcaag gaaaagaagt
    10801 ctttcggcaa gcacggttca tcgactttaa aaaaatccgt tatctactag atgaactgac
    10861 agcagaagat gattcccttt cttatgaaga aatcttccga acaaaataat cgttgtccaa
    10921 acagaaaaaa gcgattgaac aagtgaaagt tttgccttca tttgttcaat cgctttttat
    10981 ttttattctg ctgtattttc ggaatgcgca tttgcgtcag aatgcaagaa atagatcaag
    11041 gtttgcaatt cattcgtcaa atcaacattt tggatcaata catctgacgg agcattcaat
    11101 cgagcagcgg taaagttcaa gattcctttg attcctgctt ccgttaactg attgactact
    11161 tcttgtgctc tttcagcggg tatcgttaaa attgctactt cgatttgttg gatcttgatt
    11221 tgttctgaca tatcttccat tggataaacg gggataccgt ctacgatgcg cccaacgatt
    11281 tcctcattca catcaaaagc acaactgata cgtatgctat tactttgatg gaatttgtat
    11341 tttagtaaag cacttcccaa gttccctaca ccgatcagcg caacatttgt caattgatcg
    11401 tcgtttaatg tctttgcaaa aaagtgcata agatcttcta catcataacc atacccacgt
    11461 tttcctaatt cgccaaaata agaaaaatct cgacggatcg ttgcactatc tacttgaacg
    11521 gcttcactta attcagttga agacactttt ttctttccag cattatttaa aatccttaaa
    11581 tagcgataat acaaaggcag tcttttagct gtcgcctttg gaataatatg atctttcacg
    11641 aaatgacctc cacgtattgt ctaattacat ctttgatttc tccatcatag cagtatttat
    11701 aggaaaaaat caattccatt gctcagatat tgtgaaattt tattctttat tcatttattt
    11761 atcatttctg caaatctcgc agtataatgg aatttatggt agactactaa ttgaaaaata
    11821 gttttttatt tgaggagaat tcacaaagta gaaaagagga ttccatgata ttattacaag
    11881 ccaatcagat tgctcgccat tttggtgcag aagtcttatt tgaaaacata catttagaaa
    11941 tcagtacagg cgctcgtgtc gctttagttg gcagaaatgg cgctggaaaa tcgactttat
    12001 taaagatcat tgctggaatt gaagcacctg atgaaggaaa gatcgctaaa aataaaacag
    12061 cgactctcgg atacctcgct caggacacag gacttgattc caatgaaaca gtttggaacg
    12121 agatgctgaa agcttttgaa caagttagac aaatggaaga gcggatgcgc gagctagaga
    12181 ttttcattag cgaaggtacg ccagacacac ctgcttatag ttcttattta aaagaatacg
    12241 accggctgca acacgatttt tcagatcgaa acggctatgg ttatgaaaat gaaattcgtt
    12301 ccgtattgca tgggtttaaa ttcgattctt cattttatga tcagaaaatc gatactttat
    12361 caggcggaca gcgcacacgt ttagctttag ctcgcatgct tttgcaacac cctgacattt
    12421 tgattctaga cgaaccaacg aaccatttag atatcgagac actttcctgg cttgagaatt
    12481 atctgcaaag ctatactggt gcattattga ttgtttccca tgaccgttat tttttagata
    12541 aagtagtcaa tgaagtctat gaattaagca gaagaaaaat gcgccattac aaaggtaact
    12601 attcgaaata tctagatttg aaagcagaac agttagcaag tgaatggaag gcttatgaaa
    12661 agcagcaaac agaaatccat aaattagaag acttcgttgc tcggaacctt gttcgcgcct
    12721 ctaccacaaa acgagcgcaa agccgcagaa agacattaga aaaaatggac cggctggatc
    12781 gtcctcaagg ggatgaaaaa caagcaaact ttctattcag tatagaaaag ccttctggaa
    12841 atgtagtctt gcaaatcgaa gatgccgcta tcggatatga taatgtcacc ttatctgaac
    12901 caatcaatct agatatccgt cgtcaagaag cgattgctct agtaggtccg aatggtatcg
    12961 ggaaatcgac gttgctgaaa tcaatcattc accagatccc ttttatccgt ggaaaagaaa
    13021 cgctagggac aaatgtcacc atcggctatt acgatcaagg tcaagctgat ctgcatacaa
    13081 acaagacgat cctagcagaa ttgtgggatg agcatcctac cactccagaa aaagagatcc
    13141 gaagtgcttt aggtgctttc ttattttctg gcgaagatgt agaaaaacca atccctctgc
    13201 taagtggcgg tgaaaaagca cgggttgctt tagccaagtt agcgatgaac aaagaaaatt
    13261 ttttgatttt ggatgaacca acaaaccatt tagatattga caacaaagaa gtattagaaa
    13321 atgcattgat cgattatgac ggcacactgc tttttgtttc ccatgaccga tatttcatca
    13381 atcggatcgc gacaaaagtc gtcgaactct ctgaaaatgg cagcaaagtc tatctaggag
    13441 actatgatta ttatttagag aaaaagcagg aagaagaaga aatcgccgaa ttgttggcac
    13501 aagatgagcc agaatctgct cctgctccca cgaacaaatc tgattattac caaagcaaag
    13561 aacagcaaaa gcttatccgt agccttcaac gaaaaattac tcaggttgaa gaagaaatgg
    13621 cgcggatcga tgaatggatc gatcagttgg aacaagaaat ggtcgcgcca gaaaacctgg
    13681 aagatcacgt aaaactcaat gagctgaacc aagatttaga agctgctaga caagatcaag
    13741 aaacaaaatt gacggaatgg gaagaactaa gcttggaatt agaagaatat gaaaattaac
    13801 gaaaaaacca aaacagaaac cattcatgct gttttggttt ttcttttttt attttatcaa
    13861 acaaaaattc catatttacc tttttcacca ataagaagac caattactag tcattctcac
    13921 taaagcttct aaataatagt aatcacccca aatacaaggt gtatttaccc cattattttt
    13981 gggcttatta taaacagact gtgttaaaaa gccatcttct tcttccgttg tagcatatct
    14041 ttctttcaat tgtcgcataa ttttccaaac agcagactgg tataagttct tttctgtttt
    14101 gtctaataga cctgattcta ttaattctaa taatccacaa accatgatca ccatactaga
    14161 tgtatctttt tcttctctgc tagaatcatc aaagatcaaa tcccaataac aaatggtatc
    14221 ttctggcaac cgattcataa aataattggc taatattttg cttaaatcaa aatatttttg
    14281 ttctttcaaa taacgataac ttaatgcaaa accgtaaatc ccccatgctt ggcctctagc
    14341 ccaacaggaa ttatcactat aaccttgatg cgtacttcca tatcttggtt ttccatcttt
    14401 gacatccata taaaatgtat gataagttga accatcttct cttactaaat aatctgctgc
    14461 attttgaata tgagcctgag cagctttttt atatttttga tttccagttt cttcactcgc
    14521 ccaatacaat aatggaagat tcatgcaaca atccatgatc attctacctt gttgtgaagg
    14581 atcactagga tctccccaag cttgtataac tcctgccttc tcccaaaaac gctgaagtaa
    14641 tgtttctgca gcatcaattg aagcttcctt catttttcga tttcccgtta ttttataatc
    14701 tgctacggtt gataaagaat acaaaaaacc aagatcgtga tgattagtga taatttgaca
    14761 gtcaatcctg tggataaaat ctatatctgc tttttcagct gtttgttgaa ataaaggatc
    14821 acccgtcttc tcaaataaca accaattaat tcctgtccaa aatccttctg tccaaccaat
    14881 attgtctctt aatggataac gtccttttat aggacactcg cttgggaatt gatactgact
    14941 gttactgata cgtttgatta acctctccgt gtgtctgcga caacgctcga tctcattatt
    15001 ccattctttt tccattaatt ctttataaca ttcatttatc catttttctg gatgtattat
    15061 ctcttcaaac atcactttat caagcatttc cccaccagcc tttagcataa ttttagtctt
    15121 tttttattgt ttttattttg gatagttagc ccggttgtcc catcaaaatc aattttggga
    15181 cgattgttcc atacttctaa actataatcc cgatcttgat gagcaaccac tcctgttccg
    15241 agccaatggg ttccttttcc aaatgtaccc tctaatccag gaataaaagt gttttcccat
    15301 gtcatcaagt ttgtattggg caaacccata atagctgtgg attttcgttt aatagtatcg
    15361 tcacttaaac agataatacc tgaaaagcca gcttcactca ccctatagtt ttccttgtct
    15421 tcttctatga ttattccttc tttctcagga gaacattcaa tagcaaaccc tgtctcatat
    15481 agaacatatt ctgtatctaa ttctactttg tgtacgcgaa tctgccagcc ttcaaaagga
    15541 ataagccaag ttctgatttt tgcctgtaaa gtaggttccc atgtagaggc aagtacctct
    15601 ttcttacata caatgtctcg tacattattt cgaacgatga actgtccata ggttttgatg
    15661 ctctcattct taaaactggt cgtgatatat gtgtccgctc gctgaatccc tagcattgaa
    15721 tccattgcca tttctgcttt caattgattc gctcgcggta tcgaaaaacc aaattgacta
    15781 gaataagcaa attttaaata tttttcattg gtcaatgtat ggaaatttgg ccctaattgt
    15841 cccgcattca aaaaaaatgt atgtccatta tcatgttgta tgagcaaatt ggcttctgaa
    15901 agaagtttag ttccttcacg aataggcata ggcatttctt tagccgtcca atactcatgg
    15961 tcttcaggta gcgccaacaa taaaaaaatc ttattcagcc ataatggaga taacgtacta
    16021 ttatagggtt cagtcaatgt caattgaggg taggtataac caatattcag gatacctttt
    16081 tcatcaaata tttctttttg catccaccat cttatattcc gattgactaa ccccttgatt
    16141 tcacctaatt taaaaggcaa aagatcggcc cacaacatag ctgaccaaaa gcttgaaact
    16201 gcaaaacgat aaatcatgct tcgaccaaaa ggcacatttg caccatcttc cgcaaaaaaa
    16261 tgaatatagt ctctagaaaa atcaatagct cggttcttat atatctggct aatgggcttt
    16321 tctggttcta gtacgctata aatcaaacca taataatgaa atgcaaaagg attataataa
    16381 tcttggcgac ctcttgaact gtctcgatac cagccatttt gaagataaca attttctata
    16441 ttatcaaatg cttctttcag tgctgattct ttaactggat aaccaaattt ttggataatt
    16501 gttccgacaa ttatcttaaa aaattgccaa ttaccatctg cacattcata ttcaaaaatt
    16561 tgcgagaacc aatgaataat ctggcttttt tctcctgtat ctaatttttt ccataataat
    16621 tgttcatgat gatataacgc taataacatt gcgggcattt ctaccatacg ctggtctctt
    16681 tcatgtattt ctccccaata atcgggatgg gaaggatctg ttccagcttt tattcctgat
    16741 agaattaatt tcaaccatcg ttcatctaat tggtcaattg cgggccccgc tccccataaa
    16801 acccgagaga atccttccat tcctgcaatt ttatttccat atcctacccc ttgatgtata
    16861 taatcaattt gtgcgccact tgctcgataa aacggctgga gtggttgatt tatcttttta
    16921 aataagtcaa agtaatcctg tctggtcctt aatagtggta ctggatgttt cattgtattt
    16981 tctcctcttt ttactaccaa atagctaatt ccgactgttc taatcgaagt aagccttcaa
    17041 taaaaaaata atcagaatag atcagtggca cattataagt cagttctcta tggtatgctt
    17101 ctgctccgcc tccaatgatc ccatcttcat ccatattcca atttgcatag tgcttttctg
    17161 tcttttggag tatattgata gcagcttcat gatacatttt tgatcgtaca gaggaaacat
    17221 atcctgctaa ttccagaaga ccgcatgcag cgcatactcc agcactggta tcaatattcg
    17281 aatcttttac agcaaaatca atgagaggaa tataaccagt tttttcgatt ttctgaataa
    17341 aatttactgc tatcttttct gctatagcta gatagtacgg attttttgtg taacgataac
    17401 ttaacgcgaa tccatataaa gcccatgcat ttcctctgct ccaacaagaa ttctcattgt
    17461 atccttgccc gccaagtaac cgaatatatt tccctgattc tgcatcaaat tctgcgatat
    17521 gcccggttga tccatctttc ctcactagat acttagctgt tgtatctgca tgctgccgag
    17581 caattttaga gaacctcgga tcaccagttt ccctcgaagc ccaatacagt aatggtaaat
    17641 tcatcatact atcaataatc atccaaccag gtttatggtc attccatgct actaaatagt
    17701 tccctgaaat attatagcgt ccagcaagta ccgtagctgc atgtaaccct agttgtcgac
    17761 tatgtttgtt ttgagactgt tgataatgtg caatagccga tggtaagaat aaaaaaccta
    17821 catcatgatg aacgtctgaa aaatggtaca gggcagattc taataattgt tcttgttgta
    17881 ttgctgcctc tttgaacttt tctcgttttg tcttgtgaaa gagctgccat aaaataccac
    17941 tccaaaaacc attggtccac cagtcgattc ctctgtcctt tattacgtct gtatattttc
    18001 cgttgtaaga aatatatgga attttattcc ccattctgtc taattccgca tctatctttt
    18061 cagaagctac attcaaaatt tttcttgacc attccttcgt ctgttcattc atggatatcc
    18121 cgccattctt ttaattttta tgaatgtgga gttgctccta gaacgaacta gatggttaac
    18181 attcgttatt ttttttactt atttgcagct ccaatgtcat tctccttaaa tttagactgc
    18241 gcccacacat aaatcaagac cattgggatc actgaagttg ctaaaattag cataattgcg
    18301 ccataattaa ttggctcacc tgtactagaa accgtcatat ttttgataat taagggaacc
    18361 gtatatttat ctttatctga aagcatgact aatggaaata gatattgatt ccaacttccc
    18421 ataaagataa gtaaaccact agtaataatc gtaggtgata aaatcggtac agcgattttc
    18481 gtaaaaatca cccactcatt ggcaccatcg attctggcag cctccatcag tgctgtagga
    18541 aattgactgc tatattgtcg catcataaac actgaagttg tactggcaat cgaagggatg
    18601 atcacacctg cataagtatt cgataatcct aaacgactca taatgtaaaa taaaggaatc
    18661 aatgtggtaa aaaaaggcac catcctagat agcataactg tattgaacaa cagtttattc
    18721 cccataaaac gatactttgc taatgcataa cccgccattg tagaactggc tgtagctgaa
    18781 actgtactga ttacagcaat aacaatcgaa ttccaaataa tcaagggata attatatctc
    18841 gccatgatgg cttgccagtt ttttaatagc atatccccaa acttgaaatt gattccgcca
    18901 cttaaaattt cttgattact ttgcgttgaa gctacaatca tatataaata tggcatcaaa
    18961 aatattaagg caccaaacac aataatgacg tacgtaaaaa ttctgctttg tatcttttgt
    19021 ttagtcattt ttatcacctc cgaatttaaa ttgaatagcc gataatataa cagatacaaa
    19081 caccatcgtc caggcaatag cagaggcttt tccaaaatca accatttcaa aacttgtttt
    19141 atataaattg actcctacga ccaaagctga attttctggt ccattggaag tcccaaagaa
    19201 tatatttggg atattgaaat tacccaagcc attgatagtg gctaaaactg tggccattaa
    19261 aatgattggc tttagcatgg ggatcgttaa taaaaagaat tgccgaaatt tcgatgctcc
    19321 atctatttca gcactttcgt atatctcttt agaaatccct tgaagaccag ctaaaaataa
    19381 aatagtgtta tatcctacac taagccaaat catcgtgatg atcattgata ttcgtatcca
    19441 aaaaggattt gttaaccact gtacaccgtt aattcctagc gcattcaaaa atccattgaa
    19501 taacccagtc ggattgaaaa aagtcatgaa tacggaagct acagccaccg ttgatgtaat
    19561 ggttggaata taatagacag ttctaaaaaa tccttttcct tttaactgtg ctgaattcaa
    19621 taaaacagca aagtaaatag ctaatagcat cgttattgga attgatcctg ccatgataat
    19681 caaaacattc gtgtaagatt taataaacaa tggatctcgg aagatagcta gataattgtc
    19741 aaatccaaca aattcaaatg agtagccaat tttcataaaa ctaaaagcaa acgagataat
    19801 gatgggaatc aaaatcgtca caataaagaa aataacaaaa ggtgtgacca atccataagc
    19861 acttttttta tgagacgtat ctgttgcttt tttcttgctg ttctttattg tcttgcttgg
    19921 taacgaagat tgcatcatcg ttaagtcctc ctaatatttt ttaatcacca gttaatttta
    19981 cgccgtactt atgagcaaaa ttttctgctt cttttagata tgaattttca aaatcacctg
    20041 tttgccaata gttatatgca gcggtagtca aataagacgt agcatctgaa taagtggctg
    20101 gtaaaacagt atttgatgta ttttcattga ttttattcaa tagttcatag tatttttgtc
    20161 ctccttagta gtcaaacttt ttctcagctg cttctgtttg ataggccaat gagttagatg
    20221 tagccacgcc cagctccagt gcgttggcta agcattcttc atctgtcagc ataaatgtaa
    20281 gaaattgttg tgccacttgt ggatttttgg catctttagg gacataaaaa cttgatcctc
    20341 ctgatactgc accttttcct ggattttctt ttgaaaaagg aatcagtgga gccatgcgcc
    20401 atttgtcaga tgcatcagga aaattcaaag acatattcgt agcataccag ccgccagcaa
    20461 caaacattgc agattcttga aatgctttct cagcatcttg tgcaccgtag aaatgtacaa
    20521 tatctgagtc gatcatttct ttgattaact ttgcagcatg tttcgcttct ttagtattca
    20581 gacggatatt tccttcttca tcaagcaaag attgattctg ctgggctagc atgttgataa
    20641 acaattcttg gtccccagat tctggcaagg caattccaaa actaccagtt ttttctttta
    20701 atgcttttgc cacctggata tattcttccc aagaactgat ttcttcaaaa tctacaccta
    20761 tttcagcaaa tttttttggt tgataataac aaagtgctgg ggtaaaatca ctagcccaag
    20821 gaataatcgt ttcattactc gtttgttgac gcaaaatatt caatttttga tcataaaact
    20881 gatcgccata tttgtcataa aatccatatt catcagcact ataaaaagca tcttgaaatt
    20941 tttccagcac acctgctgct gaagtgtctg ccatggagat aatatccggc atatcttcgt
    21001 gtgttaccaa ttttggggtg atcatttgta ctgttgctgt tcccgaagct acattcacaa
    21061 tttttaagtc aatatttgct ccagttttgc ggttatagat ttttgcagca ttttcaacat
    21121 agtttgtcag tttgccgttt gcgacatata cttctaatgt agtggctttt tttatcattg
    21181 gatctgcgat gtttgctgtt acttcttcgt tagtttttcc acatgcactt aagctgaata
    21241 atagggtaat gtttagcaaa gctatcacca atttgtatct ttttttcatg tctatccctt
    21301 ctttcttttc aagttcttat ataaaacgct ttcacttatg tatattaacg gaattagtaa
    21361 cacaaatatt tatataaaaa aatattttac ttgtcgtttt ggcttttcat ttttgttata
    21421 atagtcatta attataaata gaaagctttt tatataaaaa gagaaaggaa agtggttctt
    21481 ttgttaacaa acaaccaatc ctccaatctt gtcaaaaaaa cactaaccta ttccatagat
    21541 acccatcttc atttatctgc tgctccctat tttcgactac taataaatga aagcgctaat
    21601 ttatatgctg aatttgcaca taaatctata gaactagata atccttccct tttattcatt
    21661 gataaacagc aagaagtcca aatcttttct actcaaaagg aaacacaaac ttttatcatt
    21721 atccagtttt attttgatga gcaattatta gcacatatta cgggacaatc tttttctata
    21781 gatcaagaaa taacatctat cattactttt ctgaaaaatg aacagttcaa aattgattat
    21841 ctaaaacata gtaagccagc tat
//
LOCUS       DACEMO010000024        21274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEMO010000024 DACEMO010000000
VERSION     DACEMO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.024"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(104..1336)
                     /locus_tag="IW728_000718"
     CDS             complement(104..1336)
                     /locus_tag="IW728_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1024097.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDSSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="IW728_000719"
     CDS             1591..2160
                     /locus_tag="IW728_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1024098.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="IW728_000720"
     CDS             complement(2338..2778)
                     /locus_tag="IW728_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1024099.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="IW728_000721"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="IW728_000721"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1024100.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="IW728_000722"
     CDS             3732..4655
                     /locus_tag="IW728_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1024101.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="IW728_000723"
     CDS             4731..5870
                     /locus_tag="IW728_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1024102.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="IW728_000724"
     CDS             5863..6663
                     /locus_tag="IW728_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1024103.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="IW728_000725"
     CDS             6663..7490
                     /locus_tag="IW728_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1024104.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="IW728_000726"
     CDS             7468..8202
                     /locus_tag="IW728_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1024105.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="IW728_000727"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="IW728_000727"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1024106.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="IW728_000728"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="IW728_000728"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1024107.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="IW728_000729"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="IW728_000729"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1024108.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="IW728_000730"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="IW728_000730"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1024109.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="IW728_000731"
     CDS             11787..13820
                     /locus_tag="IW728_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024110.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="IW728_000732"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="IW728_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="IW728_000733"
     CDS             15353..16159
                     /locus_tag="IW728_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024111.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="IW728_000734"
     CDS             16171..17391
                     /locus_tag="IW728_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024112.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="IW728_000735"
     CDS             17381..18967
                     /locus_tag="IW728_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1024113.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="IW728_000736"
     CDS             19006..21144
                     /locus_tag="IW728_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1024114.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6685 a         3614 c         4472 g         6503 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagctatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctcc
//
LOCUS       DACEMO010000025        21105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEMO010000025 DACEMO010000000
VERSION     DACEMO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.025"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..230)
                     /locus_tag="IW728_000737"
     CDS             complement(<1..230)
                     /locus_tag="IW728_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ1024115.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKI"
     gene            complement(243..3002)
                     /locus_tag="IW728_000738"
     CDS             complement(243..3002)
                     /locus_tag="IW728_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1024116.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(2999..3703)
                     /locus_tag="IW728_000739"
     CDS             complement(2999..3703)
                     /locus_tag="IW728_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1024117.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(3715..6024)
                     /locus_tag="IW728_000740"
     CDS             complement(3715..6024)
                     /locus_tag="IW728_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1024118.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(6219..6683)
                     /locus_tag="IW728_000741"
     CDS             complement(6219..6683)
                     /locus_tag="IW728_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024119.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(6683..7309)
                     /locus_tag="IW728_000742"
     CDS             complement(6683..7309)
                     /locus_tag="IW728_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1024120.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(7316..7681)
                     /locus_tag="IW728_000743"
     CDS             complement(7316..7681)
                     /locus_tag="IW728_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1024121.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(7671..8009)
                     /locus_tag="IW728_000744"
     CDS             complement(7671..8009)
                     /locus_tag="IW728_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024122.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(7999..8325)
                     /locus_tag="IW728_000745"
     CDS             complement(7999..8325)
                     /locus_tag="IW728_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1024123.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(8303..8584)
                     /locus_tag="IW728_000746"
     CDS             complement(8303..8584)
                     /locus_tag="IW728_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ1024124.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(8586..9950)
                     /locus_tag="IW728_000747"
     CDS             complement(8586..9950)
                     /locus_tag="IW728_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1024125.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(9963..10538)
                     /locus_tag="IW728_000748"
     CDS             complement(9963..10538)
                     /locus_tag="IW728_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAQ1024126.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(10504..11691)
                     /locus_tag="IW728_000749"
     CDS             complement(10504..11691)
                     /locus_tag="IW728_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1024127.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(11755..13482)
                     /locus_tag="IW728_000750"
     CDS             complement(11755..13482)
                     /locus_tag="IW728_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1024128.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(13479..13916)
                     /locus_tag="IW728_000751"
     CDS             complement(13479..13916)
                     /locus_tag="IW728_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAQ1024129.1"
                     /translation="MTTKAQRKAIIDEKARILEIMNLSDLYTITLDPLIESYLDIFEI
                     YQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGLTNKAK
                     TGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(14028..14408)
                     /locus_tag="IW728_000752"
     CDS             complement(14028..14408)
                     /locus_tag="IW728_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1024130.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(14405..14791)
                     /locus_tag="IW728_000753"
     CDS             complement(14405..14791)
                     /locus_tag="IW728_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024131.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(14793..15071)
                     /locus_tag="IW728_000754"
     CDS             complement(14793..15071)
                     /locus_tag="IW728_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024132.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            15119..15847
                     /locus_tag="IW728_000755"
     CDS             15119..15847
                     /locus_tag="IW728_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAQ1024133.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(15993..16469)
                     /locus_tag="IW728_000756"
     CDS             complement(15993..16469)
                     /locus_tag="IW728_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAQ1024134.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(16429..16776)
                     /locus_tag="IW728_000757"
     CDS             complement(16429..16776)
                     /locus_tag="IW728_000757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024135.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(16763..16948)
                     /locus_tag="IW728_000758"
     CDS             complement(16763..16948)
                     /locus_tag="IW728_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024136.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(16949..17221)
                     /locus_tag="IW728_000759"
     CDS             complement(16949..17221)
                     /locus_tag="IW728_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAQ1024137.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(17218..17421)
                     /locus_tag="IW728_000760"
     CDS             complement(17218..17421)
                     /locus_tag="IW728_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024138.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            17546..17758
                     /locus_tag="IW728_000761"
     CDS             17546..17758
                     /locus_tag="IW728_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024139.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(17791..17985)
                     /locus_tag="IW728_000762"
     CDS             complement(17791..17985)
                     /locus_tag="IW728_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024140.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(17993..18181)
                     /locus_tag="IW728_000763"
     CDS             complement(17993..18181)
                     /locus_tag="IW728_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024141.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(18808..19173)
                     /locus_tag="IW728_000764"
     CDS             complement(18808..19173)
                     /locus_tag="IW728_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024142.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(19213..19515)
                     /locus_tag="IW728_000765"
     CDS             complement(19213..19515)
                     /locus_tag="IW728_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024143.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(19515..19814)
                     /locus_tag="IW728_000766"
     CDS             complement(19515..19814)
                     /locus_tag="IW728_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024144.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(19977..20246)
                     /locus_tag="IW728_000767"
     CDS             complement(19977..20246)
                     /locus_tag="IW728_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024145.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(20258..21007)
                     /locus_tag="IW728_000768"
     CDS             complement(20258..21007)
                     /locus_tag="IW728_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1024146.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
BASE COUNT         5870 a         4498 c         3262 g         7475 t
ORIGIN      
        1 ttgattttcc cttttttagc atccacaatt cttgtttcta attggactgg atattttccg
       61 ccaacaactg attcaaaata taccttacat tctgataaat catatggaag accattttcg
      121 acaattgtag cttccataac ttcagtgttt ttattgcctt gtcgaacttg aatcatcccc
      181 acgtaattat agggttctgt tgtacttaat atgacattcc actttgccat taaatcacct
      241 ccctattttg gtggtatgac aatggaagaa attgcacttg cactataata ctggcgatct
      301 aactttccac aaatcatacc taactctgtg ttttgttcat aagtttgcat acgaccatta
      361 gctaagcctc ggataacgcc agtatgtcca taagttccgt ctgcaaacca tgaacccact
      421 tgtccacctc ttgcccaatt aataattgca ccaactacta attgatcata tctagggttt
      481 tgaatcactt tccaaccaac agcagaccaa tcataggcaa taccaatatc agatgctgca
      541 gaagtatttc ctatcacatg agttaaacca tattttgttc cagcacccat gccacaacca
      601 cccagatatc ctgaatattc tgcagacaag ccataacact gaccattgcc aattcgttgt
      661 ccaatcaaag actctaaatg ttttagtcct gcttctccag tagctcctcc tggttttaaa
      721 tctttaaatt tgttatacca gttaactgca taatcttgac gttcaggatg tgtcgctgcc
      781 ggacgttcat agtttcgttc aaaagcatat gctgcttgtc ttggatcagt acaggcctta
      841 aatccatcaa ccgtcgtagg ttgtactacg cccatccatt gtccatttgt aaatgtccaa
      901 ataagcaatc gaacttgtgc atctaagctc gttattggtt ctttaatacc tgcagcatta
      961 aataagtttt gaacataaac ttttccatcc catgttgctg gaccaacaag aggatatgaa
     1021 gaaccatccc actgaactaa tccgtatgct ggtccaccta tttgaacagt atcaggatca
     1081 aaagttcctc ctgtttcttg ttgaatgttc cccaatattc cacatgcaga ttgtttcgta
     1141 aatccgttat tacacaaaat atcgtatatt ctccaagctc tcttttctgc atctgttttt
     1201 aattcatcag gatacccacc aggtgtttct cctccgccag atggaccgcc attttgtcct
     1261 ggtatcactt ctttacctct aacagtaagt tttccctgaa tatctaaatc accgaaataa
     1321 tatgcttttc cgtttcctaa taacacaaat cctttttctg cagttggaga aattaaaatg
     1381 tattttccat cgccatttgt acgtattacc aaagaatttt cctcaagagg tgtaggtgtt
     1441 acagcatttg gaaatggatt tcctgctgaa tctgttgttc cgatcgttcc aatggattct
     1501 tttctattcc agaattccat accttttttg gttagctcca tgattttttt gttttcattc
     1561 caaatttgaa gcgtgccttt tacaagtttt aacacatcac cataagcatt aaaggaagtt
     1621 tcgaatactt ctgcatttat tgttcctact ttgatgaagt cagcaacaat ttctcctttt
     1681 gatgtgatag caataccgaa cgggccattt actccgttgt ctgaataacc taagccattt
     1741 aaattccaac gccacactcg ctttgcattc gccacatttg gagtatccat gataagaatc
     1801 tctgatggag ctttttctgg acgaaaaacg acatgcccac cagaattacc agtgatccat
     1861 gccgtcgcat tcaacacatt ttgaactaac gtttctgttc gattgtcgat tttctttttc
     1921 aattcttgtg cttgagtatt tactgccgaa gtgtacagtg ataaatcatt acctaaaaca
     1981 atatccttat atttcccaag cgttgggaaa taagtgtatt cgaccatacg ctcttttatc
     2041 tcgatatcta gctctttcgc cctaacatga gcgacatcac caaagtgtaa agacgctagc
     2101 tcttgataca tgtcaccgta ttcgagtgtg tgttccaacg ccaccatact gactgtatga
     2161 gtagcttttg gctcatggat acgatcattg tcaaataagg ttttgcccca tttgatcaat
     2221 tcatcaactg ttttacaatc tccgttttca cgtttggcaa tacgtcgatt ctcgttggtt
     2281 actccatcaa tttctaaata accatattcg ataggttctt tgtcttcatc ataatcgtta
     2341 tcaggaacac cgccaaccaa atacaaacta ttgacaattg attcttcatc aatttcttct
     2401 tcgattgctt ctaaattgat gccaaaatca atccgaaaac cattatctga tccaatctgt
     2461 tttaccagtt tcaaatcata gttatccata tcaagttccg cgctagtcac gccagtcaaa
     2521 ttttgattac cgttatttga accaatgatt gcttcaactg gcgcgacttg tcttgctgta
     2581 aattggtgcg ttgtccctac attcgaaaga tagttaaatt tttgatcaaa tgccaaactg
     2641 tttttaagat tggtcatgat ctgagaccca ttgccgttat ccgtaaacga cttgacaata
     2701 aagttcttat tggccataaa accaatatgc cgagctgtta cagaaacgga ggtcaaattc
     2761 tttttgacat tataaatttc aaaatattgc catgatccat ctggtacctt cgcttttata
     2821 aaatttcctt tttttaaata agagcgatac tgtccgcttc ttgaatagtt accataaaaa
     2881 cgatattggc cattcagcac acgattgatt tctggcaaat cttcccaatc aactaacgac
     2941 gctccgttca cgctcaaatc atctggcatt tttttataag cataaataaa ttcttgcgtc
     3001 acagatacac actcctattc caaaaccgca cttctttaaa ttttccggat atttttacct
     3061 gattccattc aggctgcaat actggccaat caccacgcgt aaataaattc aaaccttctt
     3121 gtattgcttt tccaagctga gtatcaacca cgattgttgc agctaacgta ttaagtattg
     3181 ttaggctttt atcaccgaca gcgatcgtta tatctcctcc gttcgattct atttccagat
     3241 agggatgtgc aatttcgtca ccatgatcaa aaatatccat aacactagaa tgaaaaattt
     3301 taggtgcttc accgattttt cttttgagtg gctgacaacg aaaagtaaca tcgaatgtat
     3361 aaaagaagcc ccactcgttt tcgaaaggga cttctttatc catgctgcag atagcctcta
     3421 gatacttgtc tggatcatta tgtgtgatta attggctttt accagtgagc catcgtttga
     3481 cttctctcag tttcgaatgt ggaatagtga tcccttctat ttccaaatca aaaggttcat
     3541 agtcatcaaa cgtctccgtc aattctccgc ttcggccctg aatcgtgtaa gtttcgtatc
     3601 gcttgttcgg cataatatct ggaagctctg cctcaatgat gcaattcata tcaattactg
     3661 cattccgatt tttccaaata aaatttggtt catctggatt cataaattgt cgactcaaat
     3721 aggaacacct cccaaatcac gaatagcctg tttgtttgct ttagcaaatt tacgattcat
     3781 acgatctaac tcagatggat tgtttgcatc gactttgcca atgtgtatat gctgttcaat
     3841 gctacctcca gaaactcttc cactgatccc tttgcgtttt tcttcgtctg ataatggagt
     3901 aactgtggtt ttaccattct tggcagtcag taactcaggt ccagcttcac caacaatcgc
     3961 ttgtccattg attagatgac cgccttgtgc taaataaggc agttttgaaa tagaaaatga
     4021 ttttccacct acaccaggaa cccattttgg tatctttata ttattcaatc caccaataaa
     4081 tccattgatc aatccaatca tggcattaat aggagctttc cctacagcaa caataccatc
     4141 gaaaatacct ccaaaaatat caactagacc ttgccatgct tttgaccaat tccctgtaaa
     4201 tactccagtg ataaaatcta gaaaaccact gaaaattcgt tttccagcat tataaaaatt
     4261 attgaaattt gctataacac catcgaaaaa tccaccaaaa taaccactta aaaaattaaa
     4321 tatttcaaca gctacatctg aaacaccttt gaagaaagaa ttcactccat catgaaacca
     4381 tttaacgtta ttatatgccc aaattagtcc agtaactaat gcgccaatag ctataactgc
     4441 taatgcaaac cagcctcccg acattccaaa taaaccagca agtccttgcc aaactcctat
     4501 aaaactttta acttccccaa cgatttttgt aacagatccc ataagtgaac caagaacgat
     4561 tagtactggt ccaactgctg ccgctatacc agcaattgtt atgatccaat tctttgtatc
     4621 ttgatctaga gacccccacc atttagagaa ttcctgtaat aaggaagttg ctttttcaaa
     4681 aaaaggcaaa aggcttactt ggactgcttc acctacgtca gccattgcta attttgcatt
     4741 gttcatagct tgatcggctt gatcaattgg gtcgagagta gcatcgaatg tgtctcctac
     4801 agctccccca ctttctccag cagtttttgc taaatcttcc aggttcaaag tacctctacg
     4861 aattgcatcg gccattctag gaccgccttt agtcccgaat acttctgctg ctgcattgat
     4921 ggcttctgtt tcagaacctg cattttttac cttgtcttgt agttcttcta gtccttgact
     4981 taaagatttt ccgtctttcg cataagccac tgttgcttta gataaactac ttaaagctgc
     5041 gcttgaatca acaccagatt gctcaaattg tccaagtaaa gtgacccctt caccaaagct
     5101 caaccctaat tgtttaattt gtggtgcgcc atcaattgct ttttgcatca agtcgtctac
     5161 agattgacca gtattctgcg atgttttcgt tgttacatca aggacagaat tcaaatcatc
     5221 atattctagt ccataagcat caatagcttg tcgagcagat attgctgatt gcgaaacatc
     5281 tgtatcattg atttcagcgt actgtaggag ataattggtt gaatcttcca gttttttatc
     5341 catgaatcca aattgtgtat ttacctcacc aatagcttcc ccaaccgttt gtaaaggtaa
     5401 atgagtattt gaaccaacgt ttttgaaaga ctgtgaaagt ctatcagctt gatcacctgt
     5461 tgctccggtt tttgtaatga tggtatcaag tgcctcgtca acttcaccaa atgctgcaag
     5521 tcctgctgct cctgccgcta caataggcgc ggttactcca acagacattt tttcaccaac
     5581 gccttttact ttctcacctg tttcttcgat tttctggagt ttttttgctg tatcaacaga
     5641 aacgtcacct tgttctttaa gtgcatcgtt cgtctgttct aatgctgtac gaagtttatt
     5701 ttctccggtt tcagaattga gtaattgttt ataaagtttt tctgattcag cagaatattc
     5761 accggtttct ttgacagatt tttcatattc ctctctcagc atttgagatc gttgttcagc
     5821 caatcctaat tgtttttcta atttttgttt agccgctcgc aatttttctg tttgagttgc
     5881 atccttgtcc atcgcagaca cttggttttt gtactcggca gcggctaagt tcatttcttt
     5941 gttgatttct ttgattgttt tcgaataact gacttcgcca ttcgttttaa aattaagaac
     6001 gacatcagat tctttaccag ccatttatct tagcgctcct ttctaccacc aaggcgattt
     6061 atccatagtt acagattgag gtggttcaaa atcggtgttt tgttgtaacc actgtaaata
     6121 agatttgagc cacaagttag gtgtagactt taaaaagaaa ctctcactcc aattcaatag
     6181 agtgagagca acgtaaatat aaaaactcca aggagttcct atctcttccg attctttttg
     6241 tttttctttc tttttgcttg cgctttttga aagtcttgtg gcttcttgga tttttttagg
     6301 tcttccacct gaaatgtctg gctagcaaaa atttccatac aggtactata agcagacaac
     6361 acctctccac tcattcccaa aaatttgaaa atagtttctg gatcttcctc taaaccacca
     6421 gtacgcagca tggcataaat caaggcacgc atgattttta gatcactagg cgataagtta
     6481 gcagaagaaa tttgtccttc ttttttagat agcatggcgt tcatatcaga ttcaaattgc
     6541 gaataatctc caccatacac atctgctata aattccatcg tttgcatagt aaatgaaata
     6601 gggaattctg cgccttgaat aattacaaca gcagaatttt ttaagtcttc aacgtgaatt
     6661 ccataatcag ataaccgagc cattaacccg cacctccaac tttagccaat gttttccact
     6721 gttcttcatc gtatacaggt tgagcaatga atttattaaa taattctaga gaagctccgt
     6781 ctctatttga atcaaaactt gcatacatca cattattgta tttcagtccg atggaaacca
     6841 gatttgcagt tacatcgtca atttttgttt cttcctctgc agtggcatac tcttcgtcaa
     6901 tcacattgga tagttgtgta ttaggatacc aaactgcttt tttaccgcca ttttcaatat
     6961 ttccaataaa tccaaaggcg aagtacggaa attctcgtgc ggtatttttg ctaaacgtta
     7021 ctccgctttt agcaactaat cctttgattt catccatcac ttctatcgga atacccacgt
     7081 gatccaacgc aagttcatgt tttgtttctc gcgacacacg acgaaacatt ttgctggatg
     7141 cccatttttc taatgctgtt ccattccctt tgacaccgat ttttgtggca attggcaatc
     7201 gtaaaacttc actataagtc ggtgcagttc caacagaatc tggcgttgcc atcatcgcaa
     7261 ttaaaatgtc gtccaatcct tcaaaataaa aagtatcttg ttttcccaat taaaatcacc
     7321 cttcccataa atcaagtatt tttctagtca taatttcttc aattttttct ttgttttgtt
     7381 catacgtacc acttgcaaaa tgttgggcac gttgatttgt tgtgccattt tcggcaaagc
     7441 gccagtaaaa ggccgtctct tcaaattgaa ctttcacttg atcgttttca actactactt
     7501 ttatttgatc gcgcatatgt ttctttttta agagcgactt aggaatatta ggaagcaact
     7561 gctttagata aaagttagct gcttcttcta aagattccaa cgataatttt ttcggatcaa
     7621 cttttgaaag gtttcctaaa taatccgata tttcagaaaa accattctta ttacttggca
     7681 ttttccacac acctcacata tgtgtaatag ttggttacgg tatcgtcgtt ctcatcacct
     7741 tgtatacctg taaaatctga atatggaatg ccagcatttt gcagcgcttg ttcaataaca
     7801 actaaatcct gttctgttcc aagtgtaaaa aaagagactt ggtaataagg taatttttta
     7861 tagactttac cggatgccat ttttttgctg ttactcacat ttgagtacac aatgtatgga
     7921 tacatcgtcc ctaatctggc tttgtctctg aacactgcta actttgttga tttcagcgct
     7981 gttttcaatt catcaaagct aatcgacata agctaaactc aactccattt ctcttgcatc
     8041 gggattcgta taaatgcgag taatgttata cgttacagaa tcaattttga gcgcacttaa
     8101 tttctctgtg atggatttat cccatctgac tttaattcgt ctgacaacgt ctgtcttggc
     8161 ttgctgtgat aaatattttt cttgagaagt cacaccgagt tcttcataga atattagacg
     8221 cttaaattcg taaattgtag ttggacgatc gtttccgtct gttcctgttt tgatgtctag
     8281 caattcggct ttccatctga gattattggt ctgtctcttc ggcattttga atcgctcctt
     8341 gcacgataaa tggcgtcatg gcattcatag ctttgtcgag ttcatcctct gaaactctat
     8401 attcataggc aatgccggca accatcaaaa taagatattc ttgttggcca ccagttgctg
     8461 ttttgacata atcttttgcc atatttaaat aaaaagagag caaagaatca tccatgccct
     8521 cttcaaaatg aatatgtgat ttgaattttt cctctaaaga caattcttta gtttgctctt
     8581 ccatcttaac caccaactgg ttttgtaatt tcgtagcgat atactgccgg ttcaaatgga
     8641 gaataaacca attgaccatc tagcaagttg taaatttgga atccaatttg atttttacca
     8701 gagaattttt caacaagttt ttgaatttcc aaggcaccaa taacttcttg aattttaaat
     8761 gcagaaaaat cgccaaaata taaaactggc gtgtctggtt cacccttttt atctgctgca
     8821 tctgtccaat ccacaggata gccaactaat tggtaaccaa ttccaccttc tgcttgtgtg
     8881 aatggccgca acaaaggaaa tccatcatct gttttcattt tttcaatggc agtcaaagca
     8941 gctcgattaa taataaagcg tccctttttc atcacttctg tcactggtgt atttttaaat
     9001 tcgattaatg catcatataa tttttgccca gcacctgaag cagttagatc taaaggtttt
     9061 ttaaatgcta cagccttttt ggctaatgca ccaggatttt catttccagc gtcatcacca
     9121 ttgaacatat aattgatttc tttacgcaca taagcttttt tcaattcttc cacaacaata
     9181 tcttcaactg gaacaccaga catttttaat aattttttag ttactgttgc caaagcatcg
     9241 aattcggcag gattaagcaa aatttcatca aactgaatag ctgtttcagc aatatcagtt
     9301 gaacgctctt tcttgtttac attcgcatct gctttcttca caagaattgg atatttgaca
     9361 tctcctgatg ttcgcaccac tgttccgtat ttacgaagta aattttcttc ttgagcataa
     9421 gtaataactt cagatgcaat tacttctggg acagtaactg aaccgttgcc agcttcaatc
     9481 cctaaagctc gagcttctgc ttcagaaata tttccagcta caaaattagc aaatgctttt
     9541 cgtagttgtt gatctttttg tttcttggtc atttttgcac gtgcctccaa tccgttctta
     9601 attgatccaa gtaatccatc tcgttgctgt tgagtaatca ttccagaacg attttctgaa
     9661 ctatcttcac tgtctgaatc ttcttcttga tcgttgtctt caccagaacg gctttcgtct
     9721 gaatccgtac tattcgattg atcatctgta ttgtcgtctg tttcatcagt tccagaatct
     9781 gcgcctaatt cgtctttaat tccgttcaat tcatcaataa cactgtcaat ttcttctttc
     9841 acggcttcta aatctgcttc gcgtaattct cctgattcaa ctttttcacg taattcagtc
     9901 aatcgttgct cacgacgtgc cttcattttt ttcaataatt ctttatccat gtatttttcc
     9961 tcctacgctt ctagcgcttg attaattttt ttgattaatt tttttctttc ttcgacgttt
    10021 ttttctaatt catcacgact tcttaatgca gcttctgtat cttcgtatgc tggcaaaggc
    10081 acaatagaaa cctcgtataa ttccacttca tggattgttc gaagcattgg ttcagaattg
    10141 tagtcccacg tttcttcagt cggcacaaaa ccaaaactac attggttgat gtctcctcgt
    10201 tccatagatt taactaagtc ccttgctacc gttgtgtccg gtaaatcaac ttcgaatttc
    10261 aaaccacgtt catcttcttc aaggcgaagg gttccgcttt tagtacgtcc tagcacattg
    10321 gaccaatcgt gattgaacaa acaacgaaca tccgaattac taattgcctt tgagaatgca
    10381 ccaggaacaa tcacttcgct taagtcatcc cataaaagtg tttgactgtt gaatacagca
    10441 gcgtagccgc taatggtcct cgtatgagtt tcttcatcgg aacgtgttga aagattggtg
    10501 atgtcaatcg tgcgaatttc cttcttcttc atttccatca cctcctttca agtcttgatc
    10561 attcgttggt aaggaatcat ctgttgcatt tttctggcca atcctagata agtcatttga
    10621 aatatagata gcttgtgttt ctggagtatt ctgtttagga aaaccaagca tttctgccac
    10681 gttatctgga cttgtaatcc cggtacgaac gatgttgtag ccaatatttg tttttgttga
    10741 gtaaggaaca aaatccaaaa tattaatttt ccattccact ctatagccag aattaggcat
    10801 aaaaaaaaga gcggtgtaat gttcgctctt gtttttcaat attggtttga ttgctttgtt
    10861 gtgcagatac atcatcgctt tttcaatatc tgtcttcatc agcgattgat acgtatcaac
    10921 attgattcct agaaattttc ctaagtcttt tttgtaaaca cctaaataat tcaaaattgc
    10981 cgcgtcatca acaggacttt ttaatgtctc gatggaatat ccttttccca aaggaatcat
    11041 cttaacagaa tgatcactct cattttgcgt tccttccagt tgatccaata tagctttgac
    11101 aatttttgtt tgggcgctat tatttggatt gatgtgggcg tctagtttaa gcatgaacgc
    11161 aagtaaaccg cctttagtat atttgtccgt caaaactttt tcagcgctca gaacgccttc
    11221 tagagtgttt cttgcaagat caattattcc agcacctttt aatgaatcag ttccgatgtt
    11281 cttaatgtgt cgaatcattt gaccaggtat tttttgacca ttcatttcaa attcttcttg
    11341 aagtcgttca ttgattttag ttgttacacc gtacgccaaa tgaagctgat ccctatccgt
    11401 taatgggaat gtctcaccat tgatcaataa agtatttgtt tccaatttgg taaattcgaa
    11461 tcctgtcaaa taatcattgg gactcttcaa aatatttagt aagtgatgat ctttgacttc
    11521 gttgccgtcg ggaccaataa caattggtgt ggccaatgcc acctgattag atatgtcttg
    11581 aactaattca taaacatcag aagattccat gatagatgaa tccgtcacat atctttgacc
    11641 ataacgtgtg tagtgtccaa aaatatcctc gatatatcca cgtttttcca taaaagaata
    11701 gactgcattc gataaccgat cacgtaattt caattttttt caccgccttt ctatttatct
    11761 atagatggaa cttaggtaat catctaactc atctgaatca atatctgtca tttgattcat
    11821 cgtttcctta tgaccacaca aaaacgccac gaacccatca atctttttct ttgattgacg
    11881 tttacttggc gctttttgtc cattgatgtt agtaattgct acaacgttca aagtgcaata
    11941 aaggaataat ggattatcaa attgaattcg tttctcataa aacaaccgtt cgacatcgtc
    12001 aaaaggagca ttcaatactt taggatattg agcaacttca acgcattcca atcctaagtt
    12061 ttctaatttc tcaacaagtt tgtcactcat cgctggatca taattcactt gctgaatatc
    12121 atataaattc atgcagtcct ctatgtactg caaaatctga tcttgatcaa tcatttttcc
    12181 gtcgcaaaat tcaacgaaac cttgttcagc caaatcactg taaggaacat tatcttcttt
    12241 ttctcgaaac tctaaatctt cattcggaat aaaataaagt tgctttactt taagaaccgc
    12301 tttaccatct tcatcccatg ttgggaagtt taaagataca caggtcaaat ctcgtgtacg
    12361 tgataaatct aaaccgatat aacatggttc gccacttaaa tcaccaagtt cttgtgtggt
    12421 aactaaacat ggctctactt gatcctgttc aaagaaatta tccgcgccgt tcacaaatac
    12481 atccaagtgt ttcgttagaa attcagcttt ggaatgagct gaccgttgcg ctgttttaaa
    12541 tgccgattct aaagcagata aatcgactga tattccccag ttaggattgc acatttccca
    12601 gacttttcta tccgtccaat cataattttt atttggctca tagattagaa caaagtttga
    12661 atcattatca tcacgtttca acacttcttt tgcttcgcga tatacacgca ttccaacaga
    12721 cgacgaacct ttcccagcag ttgagatatt aaacatcaat ggctgtggta aagatatctg
    12781 agcagactta aaattgtcat actgttccat tttctcctgt ttatgcaatt catcgttcaa
    12841 tacaaaatat ggattggatc cctcgatatt atcaatattc ttggtttgaa caataaattt
    12901 atttgagtaa gccatatctt catgaatata gtcataagta atactagaaa cagttccctt
    12961 tggcccccta aatattttag ttccttcaag taggattgga ttgtttaaga ttgtaactgc
    13021 aaaaggtttg gcagcatatt gcgcttgagc aaaatcagat gcgcaagcat agcaatcaac
    13081 tgataacgcc ccttctccgt acattgcata tcctaatgaa ccgacagcaa tcaatgtttt
    13141 accattcttt tttggaatct ggacataagc ctcacgagta acgcgtacaa tttggccttt
    13201 ttcattttct ttaacccaac cataaatcca agaataaata aatttttccc agggttctaa
    13261 aagaaacggt tttcccacca tttctccttt tgtgtgaaca ataaatgatt ctacccaatc
    13321 catcatttca ttcgcgcgat ctacatcaaa ccaaatatct tttctttttt tccactgata
    13381 ccagcgatct attgctaaac gaacggtctt aggatacttc ttaggtttct ttcttacttc
    13441 ttttgcaaat aaatcagcat aatttacacc aggttcaatc atttttcagc acctgccttc
    13501 ttacgccatt tatttctgtg cgctgctagt tcatctactg gctttacttc tgggcgtgta
    13561 atttcttcat cttttcttgc ggtcgatcct ccagcaatta gtctaccagt ttttgctttg
    13621 tttgttaatc ctaataaatc tagagctttc atcttcttat ctgcccaagt ttctacttgt
    13681 tgtgccaatg gatgttttga ttgattagta gctcctgatt tattcgtgaa tttttgcgtc
    13741 tccggaaatc ctttttcctt ccacaataaa tatttgtgtt ggtaaatttc aaaaatatcc
    13801 aagtatgatt cgattaatgg atcaagagtg atggtgtaca aatcagacaa attcattatt
    13861 tctaagatac gagctttttc atcaataatc gctttacgtt gcgctttagt cgtcattttt
    13921 gtatacaccc ccctttgttt tttgaaaaat ttgacctaac gatgcgcgtg actccccgct
    13981 accctatctc cccacgcgaa aaaattttga aaatgaatag gggggcttca attgaaatac
    14041 gaaggaaaaa cttttttgtc ttccgtttcg ttttcaacaa ttggatgaca ttttgaacat
    14101 aatagcatga gattgtttgg atcaagctta agcagttcgt tgtctttgat tggtacaatg
    14161 tgatggacgt gtgctctctt tccaaatatg aactgaccac accgctgaca atgaccacct
    14221 tctctttcat aaataaattg acgcatatct ttccatgctt gcgttcgata gaatggtttg
    14281 ttctcatgat gataaacaga ctttgcttgc tgcttcttct tgcgtgatct gcttgatctc
    14341 ttgtgttcag tacagtagat accctttgct attttgttcg tgcatccgtc aaactgacag
    14401 tatttcattc tacttcacga ataagattta tgatatcgcc ttttgcacgg atagcaccag
    14461 gaatatcaat accatgtttc ttagcatatg cacgcaattc ttttgcagtc atattgtcca
    14521 gttcatccgt atcttcagtg gactgatcat tagtagcttc gtctccatca aaattagcag
    14581 cagtgtttcc atcgctatca agaatttcac tgctatgagc tatgagttcg ccattgacag
    14641 caacaaatga tttgccatca ctcaaaattc ctttttcttc ttttacttca aaatttggtt
    14701 cttgatcttt cggaacaact actgtccttt tcttttctga atcccaatac tctgttccac
    14761 taatggatgt tctaattttg atcattgcca ttttaattct ctcctttgta atttgtacgg
    14821 attacttttg cgcccattcg ctcataccat tcaatcgttt cctgcagatt gggtagagtg
    14881 tgtgacaata aagaaataac taacgtattc attccagttc tgtaatcatc attctccact
    14941 tgcacatgtg catgattacc attccaattt tctctgtacg gactattctc tgcttcaatt
    15001 gctttcttat aaactttagc caaacttgat tctacttcaa tactaagtac cgcttcatga
    15061 aaatcattca tttgtttact ctcctatcag atattttgtg gtatgattta taaaaaagat
    15121 gcgaggtgtt ttaatgcttc aagaagttaa tattaagtat cgtgatcgtc aaaatacaga
    15181 gacaatttat ttgccaaata gttgtccgca ttgcggaaat ataatgactc cgtatgtgta
    15241 ttttggggtt tcatcatata gtgcgttcga taatgataga atctttggcg tattagtaca
    15301 gtgtataaat gatgagtgca acaaatttta tagtttacaa tttataaata cgtttaatga
    15361 agatgaagga tattctgaaa atattttaat taaagattat taccgtccgc cgattaaaat
    15421 agatttacca gaaaatattg aaaaagtttc tcctacattt gttgatattt atactcaagc
    15481 cacacaagct aaatctgagc aattggaaca aattgctgga gttggttata gaaaagctct
    15541 agaatttcta attaaagatt atgcaatatc taaaaattca gaggatgaag ataaaataaa
    15601 aaaaatgtta ttaggtaaaa caattgaaaa ttatttgaat gattttccta aactccaaag
    15661 attagccaaa gctgctactt ggattggtaa tgatgaaacg cactatgttc gtagacatac
    15721 taataaagat gttcatgata tggatgcatt tattaaatca gcagcgcaat ttatcgcggc
    15781 tgattatgat gctgatttag ctgatcaatt tataaatgga aataacttta tgccaactac
    15841 taattaaaag aataaaagag ccacagatac caattaataa aacggttggg atatcttggc
    15901 tctttcatat ttttttgaca ctaatagaat atcatgctgc taacaatgtt tacatagcta
    15961 caatgaccga acatttagtg aacagtgtaa ttttagaata aagagagttg ttctttgtac
    16021 ttctcgctat tgtaaaaatc aatttcgttt tcttttttca atcggtcagc ttgtctgtgt
    16081 tcatactcat ctaaaaagtt caatgtcttt ctaatttctg catgtcgctg tctgacatac
    16141 gatgagctat acccaacaga ttcagcaata tcttctaagc tcatttgatc aacatatttc
    16201 atcttaacaa tttgattatc gataccagaa aagctatcta tcagcaataa catttcttct
    16261 ttttgctcga tcaacaattc caattcattt tctatctttt ggatattctc ttctaaagaa
    16321 gaagttctcg aattcttttc gatgcggacg tttgctaaat caccacagac ccatcgattc
    16381 aattcaagct tacttttatt aagattccac tttaagtaaa gaatctgctc atctagttct
    16441 tggtaatctt ttaaccaatg aaatctcaca aacgccaccc cttatggtag aatagtattg
    16501 cgctgctatc tcgaaagggg tagctttttt attttttctt agccgttcca ttctggattg
    16561 ccttttgttc caaacgtcgt tttttcttct taatctttga ttttgttttt cccatacaat
    16621 tctcctttcc gaaaacacat tttgttcgat ttttaatact aaataaaagt tggccagaat
    16681 ttttcgttcg tctttactca tctttttttg aataagatta ctagcataaa aatagttagt
    16741 accacgaaaa tgaacaggta aatcaattac gttcaccttc ttcatgccag ttatcagcca
    16801 ctagaatctt tgcgttagtg ccgccaccgt tttctttttt gtatttttca aatgctttat
    16861 aagcagctgt ttttgattta tagatttttc cggaaccgac tgggaacatc catgtgccct
    16921 ctgtgatagc taagatgtat actttcattt aattcacccc tttaatcccc aacactacat
    16981 atccggactt ctgcatgtaa tcagtaatat aagtgatctc tgctttgtat gagtcaccag
    17041 tgtaactaaa tgttggtctc gttagtccag gtaattcatg tttaatgtat tttttatatt
    17101 cacgtagtat taactgatca ccaactttga agtcacgatc attcttgcgg atctcgaatt
    17161 gtttgcgtcc gctcgtcact gcttcaaaat attctggtag tatttttagt tcgtggatca
    17221 ttctgtttcc tccttcaaaa ttcgctcatc atcaaaaagt gaatctccac aaaatccttc
    17281 tcccaaatat ttatcgttca tgtataagtc aaccaaatat ttgtgatctg cttctcgata
    17341 ttcattaata ttaacgatct ttattttgta aacattatct tcgtcgctca ctatcttgaa
    17401 ttgttctctg actttcacca ccgttcctcc tctttttgaa aaaattttag tgtttttgta
    17461 agacccccta aatcagcttt tttatttgat ataatacttg tatcgattaa tcaaatcgaa
    17521 attaaattta aaaaggtgat ttatcatgaa tatcaacaat ttagtaaaac tcgttgaaca
    17581 acgttttaaa gaatcaggat tagaacaatt aaaaaaagat aattctccag aattattttc
    17641 taattttacc gaagacgaca ttcctactct taaacaatta aaatattatt ctgattattg
    17701 gacgcaaact cgtgagaatg aattaaattt agaaaaactc attattaaaa agaaatagca
    17761 actccagccc acacggctgg ttttttagtt tcaacttccc tccttacggg tcaaaatcat
    17821 cggttccggc ataatctgat catcctttag caacaaatca ggatattttt tccttagctt
    17881 cttattgttc gtgtaagttt tcaatagcca ttggttggct tcgcttttgc tttctccaga
    17941 aaaataaatt ttccctgttt ggatgcctgc aagttgaatc atcattttca cctcaaatca
    18001 aatttttttc tgctagatgg agcaaaagca atccgacatc aagaatcttt tgacccatta
    18061 tttcagctac tttgctcggc ttaactcctt gagaaaagag caagcgtgct tgctctatct
    18121 cttcgtgagt ccaaataaat ttggcttcct caaggataat tactggatca gttgctctca
    18181 ttgttcctct ggaaaaatga tgtcattctt atgcttggac caggtatctg catatggaac
    18241 aaagtattgt ctagctaatt cgatttgatc aattaaagca gtttctgata attcatggtc
    18301 tgcagcaatt tcattcatac tatcgccttc atctaaacag ataagaccgt tgacgttatc
    18361 gatttttaca tttgtgactg cttgactttc tccacgctca taatattcta cagttgatac
    18421 tgatacatca gcttcggagt ttaattgcag aatctgttac tgccgggata atttaatatt
    18481 cttttcataa ttcacctttt ttcctactga catcgaatat ttttcatgtt atgattagcc
    18541 gtgaaaggag agctatatta tgtgtttaca catgatgtcc ttagctcatt cggttctttg
    18601 gaagcttttt tggaaataac tctcaggttc ttgacgcaaa tctcatttat tctttgaaac
    18661 aaaaaacttc cattttccga gagctttgga tcacttcaaa agagatttgc atttatattt
    18721 ctgtgacctt gagtcgcgta aagaacctta cagcaatggc ggcaattcca ctttcaggaa
    18781 accgcctttt ttcattgata ttggtggtta gcggactacc cattttcttt cggcagtgcc
    18841 aaaccacaaa tgatctgtgt tgatgtttag gtagttgtat accagataag ctaaatccaa
    18901 agtgcctgca tcttcaagac gacaatattc caaaaattct cccatagctg gacataaaaa
    18961 tcctggagct atcatatccc aacagttaat agtgcaaaga aattcgttta gcttctgaac
    19021 gttttctttg gtttcacgaa ttacaacata ctccaattct tctccgctgg cagagcattc
    19081 caaaacttca aattgattaa gaaatggtag aacatatgct tcaaacatca acattccatc
    19141 tttctgtttt tgcacttgtt ttgcatcaat catgtctatc ctcctattca gttatttctt
    19201 ccattaatgg aattatttca ggtcactgga cttaacgaaa acaccattga ccatctttcc
    19261 agtccgacct ttgatttcgt cataagcaaa atccaagcac tcgtataaat ccatatcatt
    19321 ttgcattgcc aagatgatca aggttactac tacatcgcca attccgtctc ttaaaccatg
    19381 ttcatctttt ctagctagag aagcggcaac ttccccgatt tcttcgaccg tttttaacat
    19441 ttgcttgctg aaatccgctt gatccaatcc cttatctttt gcccactgct ctactttcgc
    19501 gattagttcg tccattattt ctcctccaca tacctaaact gtcttccttt tgaatcaatc
    19561 cataagctcc tagctctatc ccaaataata tttttgctca gaccagtaat ttcagataac
    19621 tgtttagcgg tacccgttac tagaattcga tcaccatgcc agattgcaat ctttctcggc
    19681 gttctccgtt tgggcttttc agtccacatt gatttaccga gcttttggac ttctgcaact
    19741 atttttttat cttcctgcca atcttctgaa taagtcaatt cgataattcg ctgcattgcc
    19801 gctttcttat ccatcccgac attctccttt cagtaatttg agtacttgat caagtgcgct
    19861 ctcacgtccg ccatgaaatg tgttgagcca cttgtcttcg tacgacacac tttgtcttaa
    19921 agcttcttga tgcattaatt cgatctgtgc tgtaaatgtc tttaggtcca tcttgtttac
    19981 acctgctcaa gttcattaag atgtttttgc aagcctttaa cacaatcaat gaatagtaat
    20041 ttggtatatg ctaaatttct taattgcgtt ccatcgatat agagtgcgaa atagtatctg
    20101 agtttactcc aactcgaacg atcattctta attccttcaa ttccagcttc ttcgagttga
    20161 tcatatacgt ctctcaggac gtctaattcc tcgccctttt tataattcgc tatttcatta
    20221 attagttcta gataatcgat cttcattttt ccacctctta gaatggtgct tttgattgtc
    20281 tattagctcg ttctagcgct tttttcttga gataggcttc ttggtcgatt gcccattcag
    20341 gaagcttctc tcgtcttcct gtgcgcttgt atccactgct tgcgttctta ggttcacttt
    20401 tttctttcct tgcccaactt cgaatagttg ccaaatagtt tttataagtc ttaccagatg
    20461 attcacaata ctctgacagt cgttcgattc gctcttggta atcattaggg aattctgttt
    20521 tgagtttctc catctgctca tctgacaaaa gaacattttt atactctccg tatttatgac
    20581 ggacgggctt agccttcgat tttttcgaag gcggtaactc tcttatatat tcttttgtat
    20641 tattaaatgt attattaata gatgtattat tatctttgac tttttcgtca ataggggtat
    20701 tgcgtttttc gtcaataggg tattgattaa ttcgtaggta cctattgatt atttgattgg
    20761 taccctcttt gtaaatgatt tcccgattca agtatccaaa cttaatcaaa tcacttaccc
    20821 atcgcgatat ggtctcttta ttcacaccat ataaatctgc aaagtactca ttgcctgccc
    20881 aacaaaagcc tctttcatta cacaaggccg ttatctctcc gtataacaac ttagtatttg
    20941 gtttaagtct tttgtcgtac cttacgttgg ctggtataat cgcataataa cttcgatgtt
    21001 ctgtcatttt taccctccaa tatttaactt tttgattgtt ttctggttta atttgatccc
    21061 tttgatttga tatttatttt tgaaattaat cacacctatt ttgtg
//
LOCUS       DACEMO010000026        21091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEMO010000026 DACEMO010000000
VERSION     DACEMO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.026"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            215..2101
                     /locus_tag="IW728_000769"
     CDS             215..2101
                     /locus_tag="IW728_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1024147.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2113..3060
                     /locus_tag="IW728_000770"
     CDS             2113..3060
                     /locus_tag="IW728_000770"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1024148.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3080..3607
                     /locus_tag="IW728_000771"
     CDS             3080..3607
                     /locus_tag="IW728_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1024149.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3670..4323)
                     /locus_tag="IW728_000772"
     CDS             complement(3670..4323)
                     /locus_tag="IW728_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1024150.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4455..5297
                     /locus_tag="IW728_000773"
     CDS             4455..5297
                     /locus_tag="IW728_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1024151.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5455..6351
                     /locus_tag="IW728_000774"
     CDS             5455..6351
                     /locus_tag="IW728_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1024152.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            6354..7067
                     /locus_tag="IW728_000775"
     CDS             6354..7067
                     /locus_tag="IW728_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1024153.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            7087..7605
                     /gene="msrA"
                     /locus_tag="IW728_000776"
     CDS             7087..7605
                     /gene="msrA"
                     /locus_tag="IW728_000776"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1024154.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            7602..7829
                     /locus_tag="IW728_000777"
     CDS             7602..7829
                     /locus_tag="IW728_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1024155.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            7981..8532
                     /gene="lepB"
                     /locus_tag="IW728_000778"
     CDS             7981..8532
                     /gene="lepB"
                     /locus_tag="IW728_000778"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1024156.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8615..9157)
                     /locus_tag="IW728_000779"
     CDS             complement(8615..9157)
                     /locus_tag="IW728_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1024157.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(9394..9792)
                     /locus_tag="IW728_000780"
     CDS             complement(9394..9792)
                     /locus_tag="IW728_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1024158.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            10052..10912
                     /gene="ylqF"
                     /locus_tag="IW728_000781"
     CDS             10052..10912
                     /gene="ylqF"
                     /locus_tag="IW728_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1024159.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            10905..11672
                     /locus_tag="IW728_000782"
     CDS             10905..11672
                     /locus_tag="IW728_000782"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1024160.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            11728..12585
                     /gene="dprA"
                     /locus_tag="IW728_000783"
     CDS             11728..12585
                     /gene="dprA"
                     /locus_tag="IW728_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1024161.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            12707..14785
                     /gene="topA"
                     /locus_tag="IW728_000784"
     CDS             12707..14785
                     /gene="topA"
                     /locus_tag="IW728_000784"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1024162.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            14754..16130
                     /gene="trmFO"
                     /locus_tag="IW728_000785"
     CDS             14754..16130
                     /gene="trmFO"
                     /locus_tag="IW728_000785"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1024163.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            16328..17233
                     /gene="xerC"
                     /locus_tag="IW728_000786"
     CDS             16328..17233
                     /gene="xerC"
                     /locus_tag="IW728_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1024164.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            17270..17818
                     /gene="hslV"
                     /locus_tag="IW728_000787"
     CDS             17270..17818
                     /gene="hslV"
                     /locus_tag="IW728_000787"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1024165.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            17832..19232
                     /gene="hslU"
                     /locus_tag="IW728_000788"
     CDS             17832..19232
                     /gene="hslU"
                     /locus_tag="IW728_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1024166.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            19250..20041
                     /gene="codY"
                     /locus_tag="IW728_000789"
     CDS             19250..20041
                     /gene="codY"
                     /locus_tag="IW728_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1024167.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            20123..20998
                     /locus_tag="IW728_000790"
     CDS             20123..20998
                     /locus_tag="IW728_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1024168.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         7416 a         3677 c         4425 g         5573 t
ORIGIN      
        1 ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg agtccaataa aataaaaagg
       61 taaaaatttt caagttttat aaaaaacaaa acatatcaga acaggatcga atcggtcctg
      121 ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa attttcctga agttcactat
      181 ttttatgata caatatccaa gataaggagg gaacatgttg aaagaattaa aagtaactga
      241 cctcaaaaaa acttatggtg aaaaagattt gtttgaccag atttcttttt tggtccacga
      301 aaaagaccgt atcgggttga ttgggacgaa cgggacgggt aaaacaagtc tcttaaatat
      361 tattgccggt atcgatagca gcgacggtga tcggcaaaca gtgttttatc caactgatta
      421 ccgtattggt tatctttctc aagcagctga attttccgat gaattaactg ttctccaagc
      481 agtctttcaa ggaaacagcc ccttgatcca aactgttcga gcatatgagg aagcattgat
      541 cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa cgttatgcta aagcagaaga
      601 acaaatgaat aaagaagatg cttggactac agataccaac gcaaagatta ttttgcaaaa
      661 attaggaatc agtgaattag ataaaaaaat cagtacttta tcaggtggtc agaaaaaacg
      721 agttagtctg gcgcaagtgt tgattgatga acccgattta ctattactag acgaaccaac
      781 aaaccattta gattatgaag cgatcgagtg gttggaaaac tatttgaaac aataccgtgg
      841 cgcactattg atggttaccc acgatcgtta ttttctagat agagtagcaa accgaatctt
      901 cgaactttca tttggaaaat tatacgagta taaaggaaat tatgaagctt acgtcttaga
      961 aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa aagcgcaagc ggctttataa
     1021 acaggaatta gcttggatga gggctggcgt acaggcacgt ggcaccaagc agcaagcacg
     1081 gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa gtaaaccaag aagatgattt
     1141 agagctgaac ctagcgacac aacgtttagg gaaaaaagta ctggaaatca aagatggcag
     1201 ttaccgtata aatgaccaaa cactgctaga acatttagat ttattgattc aatctcggga
     1261 aagactaggt attactggta aaaatggtgc gggaaaatcg acattgctta atattctagc
     1321 tggcagaatt cccctggata gcggtacaat gtctatcggt gaaacggttc atcttgcgta
     1381 ttatacacaa gaaaatgaag aaatggcacc agataaacgg atgattgctt acttacaaga
     1441 agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc ggagtagcag aattacttga
     1501 acgattttta tttccaagat ttatgcatgg gacactgata ggcaaacttt caggcggaga
     1561 aaaacgccgg ttatttttgt tgaaactgct gattcagcaa cctaatgttt tattattgga
     1621 tgaaccgacc aatgatttgg acatagcaac attgacgatt ttggaagatt attttagaag
     1681 ttttccaggt gctgtgatca ctgtttccca tgaccgttat ttcttggaca aggtagcaga
     1741 taagttatta gtattccaag gaaacggaaa acaagaactt tattacggaa acatgtccag
     1801 ttatttactg aagcaaaaag agacccagca gcctgctgaa aaagcgaaac caaaaataca
     1861 gagcaaggaa cctgctgaaa agaagaaatt atcttacatg gaacaaaaag aatgggaaac
     1921 gattgaagat gagattgcag aacttgaaga aaaaatctca ttgctccaag aagagatgaa
     1981 tcatcaagga gataatttca ctcgattaca agaattgcaa aacgacgtat ctgaaacaga
     2041 agcacaactt gaagaaaaaa tggcacggtg ggaatacttg agtgaatggg tagaagatta
     2101 ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa gaaaattgtt ggaagaaggg
     2161 catcgtaaag aagacagaac aggcacaggc acattaagct tattcggata ccaaatgcgg
     2221 tttgatctgc aaaaagggtt tcctttgtta acaacgaaac gagtaccttt tggattgatc
     2281 aaaagcgaat tgctttggtt tttaaaagga gatacgaata tccgctatct attacaaaat
     2341 aaaaaccata tttgggatga atgggcattt gaacgctaca tcaaaagtcc agactataat
     2401 ggaccagata tgactgattt tggccataga tgtttgacag atcctgaatt caatgaagaa
     2461 tataaaaagc agagcaagct tttttgcgag aagatcctaa ccgatgattc ctttgctgaa
     2521 acatacggcg atcttggaca catctacggc tatcaatggc gtcactggga aacaaaagac
     2581 ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa tcaaaaagac tcctgattct
     2641 agaagattga tcgtttctgc atggaatccg gaagatgttc ctagcatggc tttgcctcct
     2701 tgtcatacga tgtttcaatt ttatgttcaa gagggacggc taagctgcca attgtatcaa
     2761 cgcagtgggg atgtgtttct aggcgttccg ttcaacattg caagttatgc gttgctgact
     2821 cacctaattg cacatgaaac aggtttagcg gttggcgaat ttgttcacac attaggagat
     2881 gctcatcttt atatcaatca tatcgaacaa atgaaagaac aattgagccg agaaatacgc
     2941 gcatttccga ctttagtttt gaatcaggaa aagcagtcag tatttgattt tgagatggaa
     3001 gatatccaat tagaaggata tgacccacat ccggcgatca aagctcctat cgctgtttag
     3061 aatcagaaag gatgagaaaa tgttagctgc aatttgggca caggatgaaa acggattgat
     3121 tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat ctgaaattct tcaaacaaat
     3181 gacagaagca aatacactag tgatgggacg aaaaacattc gaagggatgg gtagccgtcc
     3241 gttgccgaat cgtcagacga ttgttttgac gagagattct tcctataaag cagaaggcgt
     3301 gcatgtcatg catagtgtgg aagacgtgct tgctctggaa aaagaaacag acggtatctt
     3361 tttcatcgct ggagggtcag ctgtttatca agaatttctg ccattttgta cgatattata
     3421 ccgtacagtc atccatcatg tattcgatgg agatgcttat tttccacctg ttgactggtc
     3481 tgattggtca ctgatcaata ctagtcaagg agaaatggat gaaaaaaatc gttatccgca
     3541 tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa agcgggacag gcgaattgac
     3601 gatataaaaa agctatggat agactagctg ttatttagag ctgtcccatc catagcttta
     3661 ttttttttat taagtaaaga ataaaacaga gaagtacata aaacctgcac ctaacataac
     3721 aaacaaatgc caaacaacat gcatatagcg gatagaattc aagctataaa agaatgcgcc
     3781 tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca ccagtcattc caagagagtg
     3841 ataaagagga acagcagcca cgacacagag ccaccccata aaaatataga tgatcgtaga
     3901 tacttttgat actctctccc gtttatgcaa cgtcaacgct ttatatataa tgccaagaat
     3961 cgcaaacaac cagattaata caaataaagt ccaacctagc catcctcgga tactcagcaa
     4021 acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc gaactatgat cgaatacttg
     4081 gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac aaagtagacg ttaaaaataa
     4141 aagaatcatc atcgatccat agatagcata agagacaata tggagggagg aatgcaagtg
     4201 ggcacctttt actaaaagaa ccactagtcc cgcgatactc aatccagtcc caattccatg
     4261 ggtgactgca ttaaacactt catttaatat ttcatacgtt cgattaaatt tcgttttttc
     4321 catgaaaacg gctcctttac taggtgtaat taatgaaaaa tcattatttg tctgttatac
     4381 taaaaataga aattgtctta catgtagttt aacatgaata aggcatcttg ttaaagaaag
     4441 agagagagaa aataatgaca aatgtaaaaa tcgtaacaga ttcttcatgt acgatggaaa
     4501 aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct atccatcatg atcgacggaa
     4561 cagtatatcc cgatgatgac caactagacg gcgaaagatt tatggaaatg atggcgaaag
     4621 caaaaaatct gcccaaaaca agccaaccac caattggaga attcatctcg atgtatgatg
     4681 agctaggaga agacggcagt gaagtgattt cgatccatat gacaaaaggc ttgagtggaa
     4741 cagtagaagc agcaagacaa gccagtaatc taacaaaaac gaaagtaacg gttatcgaca
     4801 gtgataccac tgaccaagga ttatcttttc aggtaattcg agcagctgaa ttagcaaaag
     4861 aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa agtacaagac aatacaaaat
     4921 tatacatcgg aatatccaca ctagataact tggtaaaagg tggaagaatc agccgaacaa
     4981 ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat gaatttcgcc cattctgaat
     5041 taatccctgt aactaagggg cgaggaaaga aaacgttcga taaatggttt aataatttga
     5101 aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg tatttcacat gcggatgcaa
     5161 aagaattggc gcaagaattc aaagagggtt tgcaagaatt attcccggac atgcatatcc
     5221 ctgtactgca tacgaatccg gttatcgcta cgcatactgg taaagacgcg tttgcaatca
     5281 tgtattatgt agattagtcg aaaagaggat aagccctcag cctgaaaggc ttgcagctta
     5341 ttctcttttt ctttataaaa ttaggaaaat aattgttttc ttttttcata aagactacct
     5401 ttttgataaa atttttgata acattagaac caatcgaata caagaggtga aaaaatgaaa
     5461 ttaacgaaac gacatttgat cctcgttttg ttcccattat taattgcgat catcatctat
     5521 gcaggtttag ctgcttctat accgaaagct ggcccactaa tgaaaccagt aactactgtg
     5581 agagaagatc caaataaaaa agaaaaactt cactacatag ctatcggaga ttcgctgact
     5641 gaaggagtag gagatcaaac caagcaagga ggattcgttc cccttgtcgc aaatgatcta
     5701 aaagaccgtt ataatttgac cgcggttgaa acagaaaact atggtgtaaa tggtgaacga
     5761 agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa ttcgaaacaa tatcaaaaca
     5821 gctgatttta tcacactgac tgttggcggt aatgatttga tgaaagttat ccaaaataat
     5881 ttattcgggt taacggtcaa ttcctttaaa aagccactga aaaactatca ggaaaatgtc
     5941 acagaactat tggaagaatt tcgatccctc aataaaaccg cacctattta cattttaggc
     6001 atctacaatc cattttattt gaactttcct gagatcactg acatgcagac aattgttaac
     6061 aattggaaca aaggaacaga aacggtgaca aaaaaaatgc cgaactgcta ctttatccca
     6121 atcaatgatt tgctttatca aggattgaat caggaagtag gtgtcacaga agaaggaaca
     6181 gatcaaacaa ctagcacgac agaaaatcta aatggcatca aaaataatgt gctctatgaa
     6241 gaagatcatt tccatccaaa taatttaggt tatcaattga tggcaaatgc agtgagagat
     6301 cagctgatcg agacacaaaa ccaatggtta gagaaggagg atgatcaata aacatgaaaa
     6361 caagatctga cgtatcttca aacaaaacaa agaaaacaaa ttactggaaa ttcgccttcc
     6421 ttatccttct tggggtagta cttggttcga ccgtattttt aggcagccgt atttttgcta
     6481 atcgggaacc agaactccca gaaataccag ctcttacgga aagacaagga gatcctgtct
     6541 taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat cagctttttc cttagtgagt
     6601 atcaaaaaga tagtgatatt gaatataaat tttatttgga aaatgaagca ctactcaatg
     6661 gtactttcga agtactaggt tttcctatcg atttctattt atattttgat ccgtatgtga
     6721 tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc tatcggaaca ttgagtttgc
     6781 caatcaaaga tgtgatgaat atgatcaaga gaaactataa attaccagat tggatcgaag
     6841 tcaatacaga tgatctgact atcatgctta ggttggatca attccgaatg caaaacggga
     6901 tgtatattaa agcagataaa atcgacttag taaatgatga cattcggttt agtctatatt
     6961 tgccaaaaga tacctctgat gcttcaaaca actcaaatac caacaaaact agcaccagca
     7021 gtactgaaag caagacaaaa gaaaataaaa aatcatcgga tgagtagtat tagaggggga
     7081 atcataatgg aacgagcaat atttgcagga ggctgcttct ggtgtatgat ccagcctttc
     7141 gacacgcttc ctggtatcca tacagtgatg tcaggatata ctggagggca tgtaccagat
     7201 ccgacttatg aacaagtaaa aagccaaacg actggacata cagaagcagt ggagattatc
     7261 tatgatccaa cattgatttc atatgaacaa ttagtagaac tttactggca gcaaactgat
     7321 ccaactgacg ctttcggaca attcgaggat cgcggtgaca attatcgccc ggtgatcttt
     7381 tatttgacag aagaacagcg gatcattgct gaaaaaagta agaaacagtt agctgaaagt
     7441 ggacgtttcg aacagccaat cgttacaaaa atcgaaccag cccaaccatt ttatctagca
     7501 gaagaagacc atcaagagta ttataaaaag catcctgaaa attttaaaag aaaccatgca
     7561 cgccgggctg cctttatcga agaaaattgg aggaacgaag ggtgaatcga agcttttttc
     7621 attatttaat gacactgcga ggcggtggca cacatgatgc tttatctgtt ttcgctacag
     7681 aagccggtaa agacatccaa tttcctaagc acagctctag ctacgaagaa atttctaatt
     7741 atctggaact gaatgtcgac tatttgccgt ccatggatat ctttgacgaa gcatgggaaa
     7801 aatatttaga aaataatcat gacatataag agatagtttt gtactttatg atcaaaaaag
     7861 ttttttgtcc aatagcctaa ttgaaagaat tatttttatt tcagctatct agtcgcattt
     7921 ttttgatatt tttcttataa taaagaaaga atgaaaaaaa gagggatgga ttgatagaga
     7981 ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg tattcaaata cttacttgtt
     8041 tctgtattcc ttgcgttcat gttgcgggga tttttattca tcccggtacc agtggaaggc
     8101 aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg tgatggaaaa attttcggag
     8161 atcagacgat ttgatattgt tgtttttcaa ttagcagatg gcacgattta tatcaaacga
     8221 gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg atcaattgaa gatcaatggg
     8281 aaagtagtta aggaacctta tctaactaaa aacctgaaat cagatcatgc aaatgcatcc
     8341 tatacgacgg actttacact acaagagcta acgggacaat ccaagcttcc ggaagataat
     8401 tattttgtac ttggagataa tcgaagagtt tcaaaagata gtcgttcgtt tggcacgatc
     8461 aataaaactg atattctagg taaagcaaga tttgtctatt atccacttga tgaaataaaa
     8521 tggatccagt gagaaacaat aaagcgtgca gtctgttgag actgcacgct ttattgttta
     8581 atccaaaatc agtttactac cttagtacgt aaaatcaaac agctcgttta aacacttttc
     8641 tagcctctaa agttttgata aatgaataaa caaccattat ttgaataggg atttggatca
     8701 tattcctgat gaatctacct agaaaaatta ctttgaaatc tgcaccaaac aaaacagaca
     8761 tcagccaaga attgataaaa aggctgactg ggatcataat caataattgg gcagcgatga
     8821 cggaaggcag gtttatcttt ttatgatata aaaataagcc attaataaat ccaccaagga
     8881 aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc aaatatcgtg aaatttaaag
     8941 tgttcgacaa catttcaagc aaaccagccc agatcggtcc atatagataa cccataagcg
     9001 ccatacttaa aaatccgaat gtgatttgaa aataagcagg accaatcgct atcttgtata
     9061 agatgacatc aagggctaat aaaagagcag aggtcgacaa caatcttgca ttcatttcgg
     9121 gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat attctctcct ttgtcagtta
     9181 ataataaaag gataccataa aatcaccaat gtgtcatgac acattggtga tttacataca
     9241 ctggcatcta acaattaaaa aaagggtgag aagactgctt ttataaactg taaaaaagag
     9301 attgtacgaa taaaaaaaat agaggcggag aatacttatc gtatcatccg cctttgtttt
     9361 tttgttataa aaaacgtcag attagttatc tgcttacatc ttcatccata cgggttctaa
     9421 tgtatttccg tctagatctt ggacttctaa tgaaaacatt tcttcttcag gcattcccat
     9481 atctacttcg tggaaagtac caccattttc ttttgcaatt tgagcaaatt tttttacttc
     9541 atccgcattt tcaacactaa acgaaatcaa agcaccactt gtttttttat tatcagctat
     9601 ttctttatct tttataaatt ttttataaaa atcatgagag agaagcatta tccaaaaagt
     9661 gtcatcccat accattgaac cagcttcttc agtagaaaat tcttcatttt ttttaaaacc
     9721 cagcttttca taaaaagcaa cggaacgatt gacatcagca acaggaaaat taacaaaaac
     9781 catactattc atttgtttcg tctcctttct gttttgttcg tctatcatct cttatttagg
     9841 aaacgctgtc aaaaatttag attcatatat agaaaccaaa ataaaaattt gcatcatcaa
     9901 aaaagtgaag atgtgataga atgaggactg gttcactttc acttaactaa tagatggaat
     9961 ttctttataa taaaaaagtg aatttatatt gaaaaagaaa aaaacaagta aagccgggga
    10021 atattccggt atgaaaggaa tggagagaac aatgaccatc caatggtttc cgggacatat
    10081 ggcaaaagcc cgacgagaag tttctgaaaa aatgaaatat gtggacatcg tatttgaatt
    10141 ggtagacgca cgattacctt tatcttctcg taatccaatg ctagatcaga tcattcagca
    10201 aaaacctcgc cttgttttat tgaataaagc ggatctggca gatccacaac agacaaagct
    10261 gtggcaagaa tattttcgta aacaaggcca tcttgcatta gctatcacgg cacaagaaag
    10321 taaaggaatt aaaggtctag tacctgcggc taaagaagca ttgaaagaaa aacgggcaag
    10381 agatgctgct cgggggatca agcctcgcgc tattcgggca atggtcttag gtattccaaa
    10441 tgttgggaaa tctacgttga tgaatcgact agtaggcaag aaaattgcac agacaggaaa
    10501 taagcctggc gtaacaaaag gtcagcagtg gcttcgttta ggtaatgagt tggaattgtt
    10561 agacacacca gggattttat ggcccaaatt tgaagatgaa gaaatcggca aaaaactagc
    10621 gctgacaggc gcgatcaaag accagcttgt ccatttagat gatttagcga tttacggatt
    10681 agactttttt gcccgatatt attcaggaag aatcagcgat cgttatggtt tatcaatcga
    10741 gcaagaagaa aaattggcac cggaattact gatggatatt acagcaaaac gtgggtataa
    10801 agaagactat gatcgtggca gcgaattagt cgtgcatgat attcgtcaag gaaaacttgg
    10861 acgatacaca ctggatcgtt gcgaagaaat ggagcaaaaa agtgatgact gaatccatcc
    10921 aaagtatcaa agaacgattg aaaagcatac agagaataga tgatcctaca ctagaatcat
    10981 taagaaatga tccacgcaaa ggcgtgcagc aagcactggc ttcttttgaa cgacgattga
    11041 aaaaggaagc attaattaag gaaaaatatg aagaaatgtt ttgctttgaa aagcaagggt
    11101 acgcacaagg tcaccaatgg attgccggca ttgatgaagt aggaagagga ccattagctg
    11161 gacctgtcgt ggcagctgca gttatcttgc cgaaaaatga gatcatatta gggctgaatg
    11221 actccaaaca attatcagag aaaaagcgag aacttcttta tcaacaagtt caagaaaaag
    11281 ccctggcaat cgggatcggg atcgttgatc agcagacaat cgatgatgtg aatatttatg
    11341 aagcaagtaa attagcgatg atccaagcag tagaaaaatt gtccattgta ccaagctatt
    11401 tgttgatcga tgcgatgacc ttggatttgc ctattcagca ggaaaaaata atcaaagggg
    11461 atgcacgatc tgtgtcgatt gctgctgcaa gtatcattgc taaagtatat cgagaccaat
    11521 tgatgaaaga atatgacgtg ctttatcctg gatatggttt ctcgcataat gcaggttatg
    11581 gaacaaagga acacttagaa ggacttgaac gacaaggaat tacgccaatc catcgtaaaa
    11641 gttttgcacc tgtaaaaaaa tatttacaat aaaaaacagc cgcttttacg tatcttatta
    11701 gaaagagaga cgaaaggcgg ttttatgatg tatcaaatag aagaaaattt attgaaactt
    11761 gcttatacaa aaggaatcag tagtcaagga aaatgggcac ttgctaactg gatgatgcag
    11821 tatccttata aagaaatcgg atttgaagac atcattaaga taggtaaaat aaccaaacac
    11881 cgggagctgt ttcgcaaaag ttggcaggaa attcacgcta actggggcaa aatccaatcg
    11941 aaacagtcgt ttatcacttg ctttgatccc aaatatcccc ctcagctgct tcacttgacc
    12001 tatccgccaa ttgtactgtt ttataatggt gatctatctt tactttcttg taacatgtta
    12061 agcgttgttg gtggaagaca cacttctgct ttagccaaaa aaacagttta ttatttacta
    12121 aaaccagtga tagaagctgg atatgtgatc gtcagtggct gcgcgaaagg gatagataca
    12181 tacggtcacc aagcagcaat ctctcatggt ggacggacaa tcgcagtcat tggaacaggg
    12241 atagagcaag tctatcctgc tgaaaatcgt gacttgcaag aggtgattgc aaatgatcat
    12301 cttcttttga gtgaatatcc tcctcaatcc ggcccgaaaa agcattgttt tccaatgaga
    12361 aatcggatta tcgcagcttt gtctaaagga acatgtgtct ttgaagccaa gaaaaaaagc
    12421 ggctctctga taacagcaca gcaagcattg gatcttggaa gaacagtttt ttctgttcca
    12481 ggaaatattt taacagaaca ttctaccgga tgtcatcatt tgatccaaga cggtgcagtt
    12541 tgcacggttt cgggtgaaga tatcttggca gaatttaaag aataacacca tacttttcat
    12601 ttgcttttct tgacaaacgt tttctgaatg gatatgatta gctacgattt gtatgaatcc
    12661 tcttttaata gtaggaacac gacaatggaa aggagtccat tcagtaatgg cgtataaata
    12721 tttagtgatt gtagaatcac ctgcaaaagc caagacaatt gagaaatatc ttggacgaaa
    12781 ctataaagta atggccagtg ttggccatat aagagattta ccgaaaagta aaatgggtat
    12841 cgattttgaa aacaattatg aaccccatta tatttctata cgcggaaaag gcgatgtcat
    12901 caaaagcctg aaagccgcag caaaaaaagc tcaaaaagtt tacttggcaa gtgacccgga
    12961 tagagaagga gaagcgattg cttggcattt agcgtaccta cttgggttgg atctgaaaga
    13021 aaaaaatcgg gtggtcttca atgaaatcac aaaagacgca gtcaaagcag cttttaagga
    13081 accaagaacg atcgatgtag atttagtaga tgcacagcaa gctcgtcgta ccttagacag
    13141 aatcgttggt tattcgatca gtcctattct ctggcgtaag gtcaagaaag ggttaagtgc
    13201 aggacgtgtc caatctgtcg ctttaaaaat tattattgac cgtgaaaaag agatccgaga
    13261 atttgttcca gaagaatatt ggagcatcga cggtaatttt aaaaaagctc gcaagaaatt
    13321 caaagcaaat ttctggggaa tcgacggtaa gaaaaagaaa ttaccagatg cacaaagtgt
    13381 aaaagaagtc actgctagaa ttgatggaac agacttcgaa gttaaaaaag tagaaaagaa
    13441 agagcgcaaa agaaaccctg cgaatccttt cactacaagt agcctgcaac aagaagctgc
    13501 ccgtaagtta aactttcgaa caagaaaaac catgatggta gcacagcaat tatatgaagg
    13561 aatcactttg ggtaaacaag gaacagtcgg cttgattaca tacatgcgta ctgattctac
    13621 ccggattgcc gatacagcaa aagcagaggc cgcagcattt attgaagata cgtatggaaa
    13681 agaattttct agacatgatc accgaaagat caaaaatgct caaggcgcac aagatgccca
    13741 tgaagctgtg cgtccaacga gtgtgcttcg tacaccagat gaactcaaac aatatttaga
    13801 taaagatcaa ttgaaacttt atacattgat ctggtcacgt tttgtggcta gtcagatgac
    13861 accagctatt ttagatacaa tgaaagtgac attagaacaa aatggtgtca tcttcattgc
    13921 gaacggttca aaaatcaaat tcaaaggatt catgcaagtg tatgtggaag gtagagatga
    13981 cggcaaagaa gagaaagaaa atattcttcc agaacttgaa gaaggcgatg tagtccaatc
    14041 tgttgatatt gaaccaaaac aacattttac tcaaccacca gcacgtttta gtgaggctac
    14101 gttgattcgt gcactagaag aaaacggggt agggcgacct tccacttatg caccaacact
    14161 tgatacgatc caaagacgct actatgtaaa attgacacaa aaaagatttg agccgactga
    14221 actaggagaa attgtcaatt cattaatctg tgaattcttc cctcaaatcg tcgatatcca
    14281 tttcacagca gaaatggaag gagatttgga taaaattgaa gaaggcacgg aagcgtgggt
    14341 caaagtggtt gatcgtttct ataaaccgtt tgaaaaagaa ctgacgaatg cggaagaaaa
    14401 aatcgaaaaa attcaaatca aagatgaacc agcaggattc gattgtgatg tctgtggaca
    14461 tccgatggtg ataaaattag ggaaatatgg gaagttttat gcttgcagta attttcctga
    14521 ttgtcggaat acgaaaccaa tcgtaaaaga aatcggtgtg acttgcccag tatgtcatga
    14581 aggacaagtc attgaacgaa aatctaagaa aaacagacta ttctatggtt gttcccgttt
    14641 cccagaatgt gaattcactt cttgggataa accagtgggc agaaattgtc caaaatgtga
    14701 cggatatctg gtcgagaaga aagtcaaagg cggtaaacaa gtagtctgtg taaatggaga
    14761 ttacgaagaa gacatccaaa aataatagaa aagaggttct ttcattgaaa atggtaaatg
    14821 ttgttggagc agggcttgcc ggaagtgaag ctgcttggca gatcgcacaa gcaggagtcc
    14881 ctgtccgttt atatgaaatg cgtccagtca aatcgactga ggcgcatcac acaagtaatt
    14941 ttgctgaact agtatgttcc aattcattaa gaggaaacag tttggctaat gcagtagggt
    15001 tattgaaaga agaaatgcgc cgattgaatt cggtagtaat cacttcggca gatgaaacag
    15061 cagtgccagc tggtggtgcc ttagctgttg accgcgattc tttttctgaa acaattaccc
    15121 gaaaagtcaa agagcatcca ttagtaacag tgataaacga agaaattact gaaataccgg
    15181 aaggaatcac cgtagtagca acaggacctt tgacttctca tccattagca gaaagcatca
    15241 aagcattcaa tggttcagat ggtttttatt tctatgatgc agctgcacca atcgtagaca
    15301 aagctacgat cgatatggat aaagtatatt tgaagtcccg ctatgacaaa ggagaagcag
    15361 cttacttgaa ctgtccgatg acagaagagg aattcaaagc tttccatgag gcactaatca
    15421 atgccgaagt ggcagaactt aaatcttttg agaaagaaaa atattttgaa ggatgtatgc
    15481 caattgaagt aatggcacag cgaggattca aaacgatgct tttcggacct atgaaaccag
    15541 ttggattgga agatcctaaa actggcaaac gtccttatgc tgttatccag ctgcgtcaag
    15601 acaatgctgc agcttcttta tacaacatcg tgggctttca gactcacttg aaatggggcg
    15661 agcaaaaacg tgttttccgg atgattcctg ggttagaaaa tgctgaattc gttcgctatg
    15721 gtgtgatgca ccgaaacagc tttatgaatt cgccagaatt gctgaaaccg acttaccaat
    15781 caacgaaacg cgaagatctg ttctttgcag gtcagatgac tggtgtagaa ggctatgttg
    15841 aaagtgcagc gagcggacta cttgctggaa tcaacgccgc acgcttagca aaagaaatgg
    15901 aaccagtcga gtttccacaa gaaacagcta ttggaagtat ggcttactac attactcatg
    15961 cggaaggaaa acatttccaa ccgatgaatg ccaactatgg tctgtttcct gaactgccag
    16021 agcgcattcg agacaaaaaa gcaagatatg aagcaattgc acaacgagct ttaaaagcta
    16081 atgaggcaat aaaaacagaa ttagaaaaaa ctgcaatccc aaatgagtaa ggaaactcca
    16141 tgaagagggt gagacggata gtctcgctct ttttcttgcg tttttcttaa gaatttctca
    16201 aacaaaatgt gaggaattgc gccaattgtg aacaaactta gaaatattgg ttaaattctg
    16261 acaatttatt tgtcttttta taaacgctgt gctaaattat tctctgggac aaaaggaagg
    16321 tgagaaaatg acagaagcca cgtgggaaca aaaatttttg cgttatttga ttgtcgaaag
    16381 aggatattca gaaaaaacga aagaagcata tgaagaagac attctccatt tcaaacattt
    16441 tttggaggaa tctggagacg ctgatctgct gaaggcggaa tattttacgg ttcgtgtcta
    16501 tttgagtgca ttagctgatc atcgtcctag ctatagcaga aattctatca gtcgcaagat
    16561 agccagccta cgttcgtttt atcagtatct tctcaaagaa gaagtgataa aagaaaatcc
    16621 attttcttac gttcatctca aaaagaaaaa tttgagactg ccacgttttt tttatgaaaa
    16681 tgaaatgcaa gcactttttg acagcgtagc aggagatagt cctttggatt tgaggaaccg
    16741 cgctttactg gaagtccttt acgggtcagg catccgttta agtgagtgta gcagtttgtc
    16801 ggtatcagac attgattttg attcagaggt catgttgatc catggtaaag ggaacaaaga
    16861 gagatatgct cctctaggtt cttttgctca agatgctctg caagaatatt tcagggaagg
    16921 ccgccaggta ttgatgacaa aatatcacga agagcatgat atcgtttttg tgaatcatca
    16981 tggaaaacca atcacgccta ctggaattga atatgtcttg aatcaaatga tcaaaaaaag
    17041 cagtttggat agtgatatcc atcctcacat gctgcgccat acatttgcta ctcatttatt
    17101 aaataatggg gcagatttgc ggacagtcca agaattgcta ggacatgcca atttatcgac
    17161 tacccagatc tatgcccatg taacaaaaga aagtttacaa aaaaactatc gttctttcca
    17221 tccaagagcg taaaaataag gaaaagaaca aatttaggag gaaacaaaca tggtcgaatc
    17281 accatttcat tcaaccacta tctgtgccgt cgaaaaagac gggaaatttg ctatggctgg
    17341 agatggccaa gtaacaatgg gtgaatctgt tgtcatgaaa ggcactgcaa agaaagtacg
    17401 acgtatctac aatggagaag ttgttgtagg tttcgccgga agtgttgcag atgcgtttac
    17461 tttagaagaa aaatttgaag gcaaactaaa cgaatataac gggaatctac aacgcgcagc
    17521 tgtcgaatta gctcaagaat ggcggacaca gcaatctatg caaaaacttg aagcgatgct
    17581 gatcgtaatg aatgataaag aaatgcttct tgtttcaggt acaggagaag ttatcacacc
    17641 agatgatggt atcttagcta tcggatcagg agggaatttt gcgttggctg cagcccgagc
    17701 aatgaaaaac tatgctcaaa acgacatgag tgcgaaagaa attgcagaaa atgcgttaaa
    17761 tattgccgca gatatctgta tctttactaa tcataatatt atcgtagaag aactgtaaga
    17821 ggtgagtaga tatgtatgaa ttaaaacaaa caccaagaca aatcgtaagt gaattagatg
    17881 aatatatcgt aggacagcac gcagcaaaaa aatctgttgc cgttgctttg cgtaaccgct
    17941 atcgccgttt gcagttagat gaaaaaatgc aacaagatat tacaccaaaa aatatgctga
    18001 tgatcggacc tacaggggtc ggtaaaacag aaatcgcacg acgtttggca aaaatcgtca
    18061 atgcaccatt tgtcaaagtc gaagcaacaa aatttactga agtcggttat gtcggaagag
    18121 atgtagaatc aatggttcgg gatttagtag aaaatgcgat ccaaatcgta gaaaaagagc
    18181 agtacagccg tgtatacaca agtgctaaaa aaaatgcaga aaaacgtttg gtaaaaatct
    18241 tggtacctgg tatcaaaaaa gaacagaaac aatccactaa tcaatttgaa caaatgatga
    18301 atctttttaa tgctagtcag caaaataccg ctgaaccgca agaagaagtg acagaagata
    18361 ttcgaatcaa tcgccgtaca gtcttggaac aactacaaaa gggtttactg gatagtcgtg
    18421 aagtaaccat tgaagttgaa gaccaaaaga aacctgcacc aatgatgaac aatggattgg
    18481 agcaaatggg gatcgaccta aatgaaactc taggtgcttt gacaccgaag aaaaaagtgg
    18541 aacggacggt aacagtcaaa gaagctttag agttattgat caaggaagaa tcagcaaaat
    18601 tagtcaagga tgctgatatc catagtgagg caatcaaatt agcggaagaa agtggaatta
    18661 tttttatcga tgaaatcgat aagatcactt ctaaatcaca gcaatctgga gaagtatcca
    18721 gagaaggcgt ccaaagagac atattgccaa ttgtcgaagg ttcacaagta aacacaaaat
    18781 acgggatcat ccaaacagat cacgtcctat tcattgcttc aggagctttc catttgtcta
    18841 aaccaagtga tctaattcca gaacttcaag gacgcttccc gatacgggta gaattagatg
    18901 atttgacagc tgaagatttt gtgaagattc tgaccgaacc aaataatgct ttagtaaaac
    18961 aatacgtcgc gttgatcggt acagaaaatg tgtcagttac gtttacaaaa gaagcaattg
    19021 aacggatcgc tcaaatcgct ttcgatgtca accgtgacac agataatatc ggcgcacgca
    19081 gactgcatac cattttggaa aaactactag aagatctatt atttgaagca ccagatatgc
    19141 aaatgggaga aataacgatc acagaagcat atgtggatga caaacttggc tcaattgcgg
    19201 caaatgaaga tttaagtcgc tatattttat aaaaaatcgg aggacgacaa tgacagactt
    19261 gctaacgaaa acccgcaaga taaacacact tttgcagcaa aagaatactt ttgacatgca
    19321 agcagatttg ccttacgata aaatggctgt cacactaggt ggtattcttg acagcaacac
    19381 ctatatcata ggaagtgacg gagtattact aggttataat gaacgtcacg atgtgaacaa
    19441 tgaacgaatc aaaacaatgt ttgttgagaa acaatttcct gaatcttata ctgatacagt
    19501 ggcacagttg atgaaaactg aagctaatat cccaattact agtgatatta ctgcatttcc
    19561 ggtagagtca agagagaaat atccatttgg tctgacgact gtcgttccga tctttggagc
    19621 tggcgaacgt ttgggtacga ttatcttatc tcgtatggaa caatcgtttg cagatgacga
    19681 tctagtctta gcagaatatg gagctacggt tgtcggtatg cagatccttt atcagaagtc
    19741 tcgaagcatc gaagcagatg tacggagtgc aacggctgtc caaatggcaa tcaacacatt
    19801 atcttatagc gaattgaagg ctgtacaagc tatctttgaa gcattggatg gagatgaagg
    19861 tcgtctgact gcttctaata ttgccgatag tatcggcatc actcgttctg tgatcgtcaa
    19921 tgctttacga aaattggaat cagctggaat cattgaatct cgttcgttag gcatgaaggg
    19981 aacttattta aaagtattga attcccgttt taaagacgag ttagcaaaac acagttatta
    20041 gaagtaaggg gactggggca gatagaaatg tttgttccag ccttcccatt ttaaaaagta
    20101 agtcgaagtt ggaggaatgg caatgactgt tattattcaa aatgaccagc tgatcgccga
    20161 aatcgctgaa cacggtgcgg aattgatcag tctgaaatca aaagaacatg gtttagagta
    20221 tatctggcaa ggtgatccag cgtattgggg cagacatgca ccggttttat tcccttttgt
    20281 tggacgttta aaagatgatc agtatgttta tcaagggaaa acatatcaaa tggggcaaca
    20341 cggatttgct cgggatatgg attttgaagt cattgaacat ggacaagaaa tggctagttt
    20401 catcttaagg agtacgccgg agacattaga aaagtatcca tttgattttg aattagtgat
    20461 cagctatgaa ttaggaggag acggcatcac ggttcattat caagtggcaa ataccggtga
    20521 aaaagaaatg ttcttttcta tcggtggaca tcctgctttc aatgtacctc ttgaagaagg
    20581 attgtcattt gaagatttct atctggcatt ttctccattg aaatcaagat tacgcctacc
    20641 attgaaaggc ccattcatcg atatggatca aaagacgtta ggtcagacaa atacgaatct
    20701 ggctttgaat cgtgaacttt ttgagcaaga tgccttggtt tttgaaacaa ggggtctaaa
    20761 tgcgtacgcc attcgaagtg aaaaatcttc ccatagcgta actttaagct ataaagatat
    20821 gccttatgta gggatctggt ctccttatcc aaaagaagcg cctttcgttt gtatcgaacc
    20881 gtggtgcgga atcgctgaca cggtgaacag tagtggaaac ttgatggaaa aagtagggat
    20941 caatcaacta ggtgcccatg aaatcttcaa gacaaaatat tccatcacag tgaagtaaga
    21001 ggttgtgacc gagaagttag cagggaacac cgtttaccaa ggtgtgtaaa aaagctatcc
    21061 tgtaagtaat aagggagtgt gacatcgatt t
//
LOCUS       DACEMO010000027        20903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEMO010000027 DACEMO010000000
VERSION     DACEMO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.027"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..536)
                     /locus_tag="IW728_000791"
     CDS             complement(93..536)
                     /locus_tag="IW728_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1024169.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(754..2094)
                     /locus_tag="IW728_000792"
     CDS             complement(754..2094)
                     /locus_tag="IW728_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1024170.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2363..3259
                     /locus_tag="IW728_000793"
     CDS             2363..3259
                     /locus_tag="IW728_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1024171.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3409..4350)
                     /locus_tag="IW728_000794"
     CDS             complement(3409..4350)
                     /locus_tag="IW728_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1024172.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4476..5597
                     /locus_tag="IW728_000795"
     CDS             4476..5597
                     /locus_tag="IW728_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1024173.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5855..7357
                     /locus_tag="IW728_000796"
     CDS             5855..7357
                     /locus_tag="IW728_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1024174.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7424..7951
                     /locus_tag="IW728_000797"
     CDS             7424..7951
                     /locus_tag="IW728_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1024175.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8162..9223
                     /locus_tag="IW728_000798"
     CDS             8162..9223
                     /locus_tag="IW728_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1024176.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9226..10122
                     /gene="murQ"
                     /locus_tag="IW728_000799"
     CDS             9226..10122
                     /gene="murQ"
                     /locus_tag="IW728_000799"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1024177.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10136..12118
                     /locus_tag="IW728_000800"
     CDS             10136..12118
                     /locus_tag="IW728_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1024178.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12183..12629)
                     /gene="nrdI"
                     /locus_tag="IW728_000801"
     CDS             complement(12183..12629)
                     /gene="nrdI"
                     /locus_tag="IW728_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1024179.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12840..14264
                     /locus_tag="IW728_000802"
     CDS             12840..14264
                     /locus_tag="IW728_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1024180.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14445..15218)
                     /locus_tag="IW728_000803"
     CDS             complement(14445..15218)
                     /locus_tag="IW728_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1024181.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15215..16120)
                     /locus_tag="IW728_000804"
     CDS             complement(15215..16120)
                     /locus_tag="IW728_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1024182.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16274..16594)
                     /locus_tag="IW728_000805"
     CDS             complement(16274..16594)
                     /locus_tag="IW728_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1024183.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17068..17754
                     /locus_tag="IW728_000806"
     CDS             17068..17754
                     /locus_tag="IW728_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1024184.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17756..18700
                     /locus_tag="IW728_000807"
     CDS             17756..18700
                     /locus_tag="IW728_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1024185.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18899..19543
                     /gene="pcp"
                     /locus_tag="IW728_000808"
     CDS             18899..19543
                     /gene="pcp"
                     /locus_tag="IW728_000808"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1024186.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19622..20899
                     /locus_tag="IW728_000809"
     CDS             19622..20899
                     /locus_tag="IW728_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024187.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6753 a         3767 c         4344 g         6039 t
ORIGIN      
        1 cttattcacg gcgttccaga agtagcttct tcttatttct tgaagctgat aacttctgga
       61 acatcctctt tttttatttg gacggataat ggtcattcca tttctttgat catttttttc
      121 attttgtcga tctcttcttc ttgtgcctga atgatttctt ccgctaattt ttttacttca
      181 ggatctttta aatcagctcg tttacttgcc aggatagcga tagaatgatg aggaatcatg
      241 gccttcatcc aagctgtgtc gttgacagta gtttggcttc gtaccataaa caaggaaccg
      301 gcaaaaacaa gaagactagc ggctaagatc ataacattta tctttttgtt ttcatacatt
      361 ttccacatga acaacagcat gatgactgcc atgactgcac ccatcatcaa cgccatgaat
      421 actcgtgtac gactgaagaa gatatgatcg agcgcataga cattcaagta catcatacca
      481 aacataacta ccgttgaaac accgatcatc aacaaaaact tccaatactt tttcatttaa
      541 aacaaactcc tttttttcat tcctttcttc tatcatgtca tgagcatgga gaacaatcaa
      601 ttattttgca cagagggttt ttgaacaccg tttaccaaga ggtcatgaga ctgtttattc
      661 ggaaaaagga gttgcaacaa gacttttgtc acaactcctt tcttcaatag ctccatctat
      721 tccacataaa taaaataaaa ttttctatcg atttcattca ttcgtcaata cgaacgcatc
      781 attctttttt tgataactga aataaatgaa caggtaaagc aggccagtca atacagttac
      841 cccaatggag ttcgtgaaca ggataccaag gaaaatcaat cctaagacag ctaatgcaaa
      901 aaagctgatt cctttatccc ataatgttcc ttttcttagt gccagtacaa aggatgccgc
      961 actgataatg atccagatca ataatactgt atacgaaagg gaacctgcca gatagccaaa
     1021 taatttgtca ccgacaaaat aagaaagagc tacaccaaga taaagaacac tggtacaaaa
     1081 taaaacagca cgttgcggta cttgatgctt gtttaaaaca gctagttttc tagaggaacc
     1141 ttttcgatcc ttcaaacgga agaacaaaat tcttgaagat gcatacacac ccgaattgat
     1201 agatgagaac aaagcaagga caatcacaaa attgacgata tccgctgcaa atgggatatt
     1261 cattttttca aataccatca caaaaggact gacattcgtg cctgctaaag tattccaagg
     1321 aaaaagaatc aaaagcaaaa acaaaggaat gatgtaaaag gaaatgattc ttcccatgac
     1381 acctttgatt gctttaggaa tcgcacgttt aggatcatct gcttcgctta ccgtgatagc
     1441 aatcagttca gaaccgccgt aagaatagat aactacaagc aaagaactga tgattccctt
     1501 cataccatga ggagtaaacc caccatggtc agtcaattgc tgaagggtag ggaaaacgcc
     1561 tgtgcccaac atttgctgac cgacaagata agtagcaaaa atgatcagta agataatgac
     1621 actgatttta gcaaaagcca gccaatattc tgtttctgcg aataaccgta ccgaataaag
     1681 attgatgatc gtagttaaaa cagccaacat aaaaacaaat acccaggctg gaatgtgcgg
     1741 gaaccacaac tgcaaaaagg aagcagctgc aacagcttca gcgatgatat tgataatcca
     1801 catagaccag tagacccaat cagtaaaatc agcagaatga gcacctagat aagggcgtat
     1861 caaaccagat aatccgtgat gcgtgtcgcc accgcttaaa acgatttttc ccagaccttt
     1921 catcacgata tataaaatca gcccaccaat caaaaaagca atcaatacag aagggccggc
     1981 gatcccaatc gcttcgccac ttcctttaaa tagacctgca ccgattgctc cacctaaagc
     2041 taacatggtg atatgtctgg agctaagttg ttttttcaat tttttatctt ccataaatga
     2101 cctctttctt ttaaattcac aatcaaaatt tatattaaga ataaatagca atatttaaca
     2161 atgactaatg ataacaaatt ttcagacaat tacaactatt cagaaaactt ttaggcgaat
     2221 tttaatagaa gaatgattta tttcatcata ttttcataca aatgttaaat tttcatacaa
     2281 atataggaaa cgttttactg gctttttact atttaaacag gctatcatag gaaaaagtga
     2341 cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg caaaaaataa
     2401 atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa aagatttact
     2461 acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc tgatcgtttt
     2521 tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa
     2581 aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc tgaagcaggc
     2641 acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag caaaagaact
     2701 tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt ctagcgaacc
     2761 aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag cagaaagcta
     2821 taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc cacatcgagg
     2881 gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag gggaatcgcg
     2941 cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga catttgaatc
     3001 catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt atcattttta
     3061 tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac cggaatttct
     3121 gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg cagaaaattg
     3181 ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat atgacctctc
     3241 ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg aaacaaacaa
     3301 gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta ttgggcttaa
     3361 aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt attctgcgcc
     3421 aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat
     3481 atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt cagtcaaacg
     3541 atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt tgtcaggatc
     3601 ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc cttcttttgc
     3661 tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg ctttttgcgc
     3721 gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg ttccatttac
     3781 acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat ttaaatttgc
     3841 ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt ccaagttctc
     3901 tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta gaccagctgt
     3961 ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca gtacatcttc
     4021 atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa tggattggat
     4081 cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca agtccacgtg
     4141 ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga ccacttcttt
     4201 accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag caggccaacg
     4261 atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc ggctgcccat
     4321 tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc aatgagttcc
     4381 cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa
     4441 aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg gcaattgatt
     4501 catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat ggctgggaag
     4561 atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat actgcagaag
     4621 caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat ctcttattcc
     4681 gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg
     4741 aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa actatccgac
     4801 ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa gaagtagaac
     4861 aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga ttactccaat
     4921 ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct actaaaaaac
     4981 ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc gctgcagatg
     5041 ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga caaaaaggag
     5101 gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct cagcaaataa
     5161 aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca ggcggtattg
     5221 gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa
     5281 tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga
     5341 tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat
     5401 atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg
     5461 attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg
     5521 aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca
     5581 ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata
     5641 ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag
     5701 actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt
     5761 cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa
     5821 ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct
     5881 gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga
     5941 cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa
     6001 tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta
     6061 tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac
     6121 ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat
     6181 cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact
     6241 tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat
     6301 ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt
     6361 cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag
     6421 tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga
     6481 tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac
     6541 agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt
     6601 cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc
     6661 acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa
     6721 agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc
     6781 tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt
     6841 cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg
     6901 tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg
     6961 agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa
     7021 tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa
     7081 caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag
     7141 tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata
     7201 tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact
     7261 gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa
     7321 agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca
     7381 aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag
     7441 acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct
     7501 gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt
     7561 tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt
     7621 tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg
     7681 ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc
     7741 tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt
     7801 gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat
     7861 aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt
     7921 agataataca gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata
     7981 tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tcttttgata
     8041 atataaactc aacctttaga aatgaaattt aattatttta ataaaagtat tgaaattttg
     8101 tttctttaga tatatactct agtcatagaa atgaagacgg atacagaaaa agagggataa
     8161 tatgtttggc ttttcaatat ttatgaacga ttctttgtca gaagagaaga aacagtatat
     8221 ccgccaaatg gcatccaatg gctttgtcgg tattttcact tccatgcata taccagaaga
     8281 tgatgtttcg gcatatcaaa agcgattgat cgaactagga agcgaagcga aaaagaataa
     8341 actagaattg atggtcgata tttcaggaga ggcattggaa cgagcgggtt tttcattaga
     8401 agatctaacg cctttgaaag caattggtgt aacaggttta agaatggatt atcatatttc
     8461 caatcagcag attgccgact ggtcacatca attgaaaatc agtttgaatg ctagcacgat
     8521 cacgcctaaa gatatcgatg aattgaaaga agcagaagct gatttctcac agattgaagc
     8581 ttggcataat tattatccac gtcccgaaac agggctggac aaagaatggt atcagaaaaa
     8641 aaatcaatgg ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag gagatacaga
     8701 gttgagaggc ccactatatc aaggattgcc aactttggaa gaacatcgag ggatccatcc
     8761 tcttgcaggc gctctagatc tattagcaag taacacggat attgtttaca tcggagatgc
     8821 aggtctgtca gaaaacgttc aggagcaatt tgcttctttc caaaaagaac agacagtctt
     8881 gctccatacg gaacctgtag atgaagaatt ttacgaatat atattaggga agcatacgaa
     8941 tcggcaagac gatgcaagag atgtcatacg aagcgtagat gcacgttttc gagagattcc
     9001 gcctatccca gcaagaaata cagctacgcg tatgaaaggg tctattactt tagataacga
     9061 aaagtatttg cgctatatgg gtgagatcca attaaccaaa tatgatttgc cagcggatga
     9121 aaaagtgaat gtagtagcta aagttatcaa agaagagcta ccattgatca accaaatcaa
     9181 agctggtatg aactatcaat ttataagaaa agaaggaaga taaaaatgag caaaatcaat
     9241 ttagagaacc taacaacgga acgcagaaac caagaaactt ttggattaga tgagatgagt
     9301 atttctcaag ccgttcaact aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa
     9361 aaagaactga caagtatcga accagtgatt gctaacacga ttgagtcctt taaaaaaggt
     9421 ggacgtctga tttatatggg agccggaaca agtggaagac taggtgtgtt agatgctgct
     9481 gaatgcgtac cgacattcgg tgtagaaccg gaaatggtca taggtctaat tgctggtgga
     9541 gaagaagcaa tgactgtagc tgtcgaaggg gccgaagatt cttatgagct agggcaagaa
     9601 gatctaatca agctgaattt gacaaaaaat gatatagtta ttggtatcgc agctagcggt
     9661 cgtacaccat atgtgatcgg tggattagat tacgcaaaat cgatcggtgc tgtcacagga
     9721 acgatttctt gcaataaaca agccgaaatc agtaaacacg cggatttccc aatcgaagtc
     9781 gatgcaggtc cagaattttt gacaggctct actcgattga agtcaggaac agcacaaaaa
     9841 cttatcttga atatgatttc tactatcagt atgattggta taggtaaagt attcaataac
     9901 ttgatggtcg atgtcaaacc aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc
     9961 atgcaagcga cagaagctga ttacgaaaca gctgaaaagt actttgaaga agcagatcaa
    10021 aatgttaagc tggctatcgt catgattttg acaaatagtg ataaagagga agctcaagca
    10081 aaattagctg aagcaaacgg atttgtaaaa gggaccttat gagggggaaa agaccatggc
    10141 aaaaaacgaa ctgactttgg aagaactggc aaaacaaatt tataaagggg caggcggcat
    10201 gggcaatgta gaaagcattg tccactgcat gactcgtgtg cgtatgaaga tcatcaatga
    10261 tgatcttgta aatgtctctg aattaaaagc gattccagga gtgcttggtg tagtagaaga
    10321 tgaacagctt caagtcatca tcggacctgg gaaagtgaac aaagtagcac agcaaatggt
    10381 tgatttggca ggcgtgaaat taggagaaaa actttcttct gtaagtacag ctacagcaaa
    10441 ttcatcagca tctggaaaag accgtgtaaa cgaacgggca caagaaatga aagcggcaca
    10501 aaaatcgaaa caaaaaccat ctaaaatcaa atcggtatta aaagacattt ctaatatctt
    10561 tgtgccaatg attcctgcat ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt
    10621 agcaaactta gtcacagccg gaacacttga tgcagcaaca tggcaacaat atatcgatgt
    10681 gatgaacatc ttgaaaaacg ggatgtttag ctacctagtt atctatacag gtatcaatgc
    10741 tgcacaagtt tttggtgcaa caccaactct tggtggagtt atcggggccg ttgtgatgct
    10801 gacagggatg aatccggaag caccactgaa aaacttattt actggtgaag ctcttgcagc
    10861 tggacaaggt gggatcctag gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa
    10921 aaaactgcat aaagtagtac ctgatgcggt agatatcatc gttacaccaa ctctttcatt
    10981 gattgccatc ggtctgattg aaattttctt catcatgcct ttagcaggtt tcatttctga
    11041 tggcatggtc ggtgggatca actgggtact tggcgttggt ggagcatttg ccggcttcgt
    11101 tctaggcaca ttattcttgc cgatggttat gttcggactg catcaaattt tgactccaat
    11161 ccatgtgcaa atgattgatg aaactggtcg tacgctattg cttccaattc ttgcgatggc
    11221 tggtgcagga caagtgggtg cagcattggc attatggatt cgctgcaaaa aagataaaga
    11281 attaacagaa atgatcaaag gagctttacc agtcgggatc ctaggaatcg gcgaaccatt
    11341 gatttacgga gtaacattac ctctaggacg cccatttatt actgcttgta tcggtggagg
    11401 tatcggcggg gcagtgatcg gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc
    11461 aggagccgct ttgatcccat tgatctctga cggtaaatgg ttaggctaca tccttggatt
    11521 attggcagct tatgctggtg gcttcgttgc tacattcttc ttcggtattc ctaaagaccg
    11581 tttagaaaaa gaagaattag cgactgaaac agccacagta aatgaagtgc ctgaaaaagt
    11641 agtacctcaa acttcagctg aaagtgtgac tatcctctca tcagttgctg acggaacagt
    11701 tgtttcttta gataaagcaa gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta
    11761 ctttgtagaa ccagaaaacg gacatatcta ttctccagtt actggggtag tcagctctgt
    11821 cttcccaacg aaacacgcta ttgggatcac aacgccaaat ggtttagaaa tcttgcttca
    11881 tatgggaatc aacacagttg acttaggcgg tacgccattt gatgtgaaag taacagaagg
    11941 gcagcaagtg acacctgata cgcttgtagc agaagctgac ttagaagcga tcaaagcagc
    12001 aggcaaagaa acatccatga tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt
    12061 cttggaaaaa acaggtaaag caaaagcaaa agcacctgtg atggatgtca aagcttaata
    12121 tgatagaaac aatagaaacg aaagaagaaa ctgaaatcta tttttcagct tcttcttttt
    12181 tattatttat tgttttctgc taaaatctga taaattcgtg tcacatcaga tggtgttccc
    12241 cgtaattcat aatctgccaa gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt
    12301 agacaatact gattattgaa gtttttattt ccgctaccga ctacaccaag acaaagttta
    12361 tgattgtctt catgttccag atattcacgc atcgtttcgg tcaagatttc tgtatcaccg
    12421 ttatctaaac catttccgcc gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt
    12481 tctttcgtaa aatcactatt ttcatggatt tcttttaaag taacttcagg attgacagga
    12541 tcttctgcat gcattttttc agcatattct gctagatgtt tggcaaaggc acgcgtattt
    12601 cctgaaatcg atatatataa aatgttcatg gatcatcctc caattctttc cttttagtat
    12661 agcatctttt ttaagttttt tggtggtgta atttggaaag ggactaggcg ctttgcttgt
    12721 ataatgagaa aaaagccggt agaattatat ttctattaaa gtttttatta attaagtaaa
    12781 aataaaaaag gtttgttata ctttttgata aaagcggttt tattttggag gatgcttaca
    12841 tggatacaga gcaacagaaa aaaggaattg gtttaattcc acttgctgcc ttagtaattg
    12901 gttcagcgat tggaggtggc gtatttggta ttatgacgga catctccgga tcggctggcg
    12961 gcccagctat tttcagttgg gtactcgtag gaacagcaat gttgatgctc tccttgtcga
    13021 tcaataatat caataaaaaa cgtccagatt taaaaggcgg gatctttagt tacgcagaag
    13081 aaggttttgg ccgtttcgct ggttttatca gcggttgggg ttactggctc acatgctggt
    13141 taggcaacgt agcttttgca acattgttga tgagcgcatt aggatatttc ttcccagtat
    13201 ttgaaggtgg gcaaaatctg gcatcgatcg tgatgagtac agtcttttta tggggatatg
    13261 catggctagt gaatcgggga gtcgaaaatg ctagtttcgt caatgctatc gtcacattct
    13321 gtaaattagt gccattattt ttatttattg tagtagctat cagcacgttt tcgatccaac
    13381 aattcattga taacttttct agcgacatta tattgaatgc gaatggacag ccgatcccgt
    13441 tttccagtca agtattgagt tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag
    13501 gagcctctgt cgtaggttca cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc
    13561 tagggatatt cggcttgatt attatttacg tcttggtttc catgcttcca tatgggacaa
    13621 tgacgatgaa tcagctgcaa acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa
    13681 tgatggttgg taaatgggga gcagtcgtga tcaacggcgg tttgatcatt tctttagttg
    13741 gtgtttggct ctcatggacg atcttaccga ttgaaacaat gggaaacatg gctgaagacg
    13801 gacttttgcc taaagcatgg ggaaaactca acaaaaatgg ggcaccaact tatgctatcg
    13861 tattgacgac gttatgtacg aatgtctttt tactttcatt attattcact gacagtgctt
    13921 ataactttgc ttacacactt ggtacagcag ctattttctt cacgtggctt ttcttaggat
    13981 tgtaccaaat gaaactttct tatcagcgga aagaatgggt ccaattttta gtagggcttt
    14041 tagcaagtgc ctttcaagta tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg
    14101 ttcttgttct tttcttgcca ggcatttatt tttacctcaa agcacgtaag gagcaaaacg
    14161 agcctgtaca acatatccac ttagataaga ttttacttat aggtgtgacg atggtcggag
    14221 tgattgcaat ggttttattt gccactggga tgcttcacgt atagaagtat aaaagaagta
    14281 taaagagagg ttgtgagcct gtcgtttatt caaaaacata ctaggaaagg agctgtgaca
    14341 caaatgttgt cacagctcct ttttcagaat aaacggtgtt taagagacag ccaatcggca
    14401 ataaggctga acgtctcttt cacagcctct tttttgatga gtcatcatac attttttcgc
    14461 ttaaatacga gatatcctaa tgctaagaac aaaacgatgt aagctaaatt accgaatagc
    14521 atttgattag tagaaagatg agtcaattgc ttcatgatct cttcgtttcc aatttggttt
    14581 tgcagattca acatactgat cggattccat ttgatccact cccatttttg gatgagtgca
    14641 ataaggatac cagatatgat tgacgaagca aaataaaaga cgattcctgc tgagatggat
    14701 gcgccggaac tattgataaa gcaagcaatc atcaacacta aacttaaaat cagccataaa
    14761 ccgatataac tacccaaagt ataagtgaac aagctttgga tcagtgtttg accagtagaa
    14821 accttctccg tgaaggaaat cgaagggaaa agaatcagtt tcatcaacac agtgacaata
    14881 atagctaaaa ggtataaata aacagaataa ataactaatg tgatccattt gcttactaat
    14941 atttctcctc gagaatactt tcgataaagg agatttttca atgtaccata ttgtgcttcc
    15001 atcgaaatga tcgtgcttgc agctgcaatc atgataaaga caatccaaga tgtggcacta
    15061 aaaagctgag cagtcatttc aactggatca atgatcgtcg tgtatttttt ggctagaagt
    15121 gctgttccta tgagcaaaac aactaataga acagataaaa cagcagttga ctttttcttg
    15181 atcaatttaa ataattcttg ttttacaagc aatgtcattt ttcttcttcc ccctttactg
    15241 aatccagaat ttccaataga gaagattcca aatcattcgt cagatgctgg acgtctgtca
    15301 catggatatt ttgtgcagac aatagctgga tcacggttcc taataattct tcgttttctt
    15361 ttaaaagctt gattttatca ggatcttcca aagtatatcc agcttctgtc aagatcgttc
    15421 gagcagctag atcatttgtt gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt
    15481 cagccattgt acattgtttg atgatctttc cttgattgat caggatcagc tcatcgacca
    15541 ttttttcaag ctcgctgagt aagtgactgg agatcaagaa agaggtgccc tgagtagcca
    15601 agtcggtaat gatttttcgc acatctcttg ttgcttgtgg atccaaaccg ttcatcggct
    15661 catctaaaat aacgatagac ggatggttca gtaaggcaag agcaattccc aatttttgtt
    15721 tcattcccaa cgagtaggat ttcgcttttt tgtgaatgta cttttccatc ttgagctgat
    15781 tcacgatagt gtcgatagca gatacatcgt tggtttcaga gaataacttc agatgatcat
    15841 agcctgttaa gaatggatag atacccgggt attcgatcaa tgctcctact tgttccaacc
    15901 ctttatgact agagggagaa atctcatttt caccaataca gattgttcca tctgagtaat
    15961 ggatcagtcc taaaatagct ttcatgatcg ttgtttttcc agcgccatta ggacctacta
    16021 atccaacgat tttgccaggg gataagctaa aactgacatc gctgagggct tgtttgtgac
    16081 caaagttctt ggataattct tggaccgaaa gacttttcaa aaatattcac tccttttcta
    16141 tagatagtac aagcatacac ctgatgagtg gagaagtcgc taggtcattg gacttatttc
    16201 ttattttttc ataaaaaaat tgaatattaa ataaaaaggc ttattttttc tgatcagtac
    16261 ttattccaag tgtctagtgg ccgctagtaa tcttgatgcc aatgatactg atgagtagca
    16321 tccccacaaa gaaccaagtg gaaagattca gcttatcatg aaacaagacc acaccaatta
    16381 tgaccgaacc aactgcgcca atccctgtcc agataggata agagagactc atcggcaaac
    16441 ttttgattgc ctttgacaaa aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag
    16501 aataattgat tttagaaaaa ccatcactcc atttcatcat cgttgaccag aatacttcca
    16561 aaattcctgc tatgattaat tccatccatg ccatacttac attcctcctt aactataaaa
    16621 aaatacacaa aaagaggaaa atcatccctt caaagaccta atggaatgat catcctcttt
    16681 atcctacccg gtggcagatt gtatatcttt ttctgtagta tagcacacct atccagatat
    16741 cggcaatctc atacttttta gtaaagtgta ccaagcactg acggatatat cgttagcgga
    16801 aaagaagatg aatgctttgc aatttcatag aaagaagttg ttatttatta atcatttaaa
    16861 aaaaacagta actagacctt atttctatat attatcgggc aataaattaa aaaaagcttt
    16921 accttacaat aagtaagata atttttgtga taaaaaaata acgaaaaata tttaaaaata
    16981 gtgtctattt cgtgagaaaa caactatatt ttttatgtgt tttgctttat aattaaaaaa
    17041 atattgtcat tttatggagg aaaatatatg gaaaattatc ttagcctttt aggcgtcttg
    17101 attgttattc tcgggtttgc atttaaatta gactcgatat tgatcgtaat ggttgcttta
    17161 gttgttacag ctttgactgg cggtcttggc attcaaggaa tgttagaact tttaggtaca
    17221 agttttgtaa acaatcgcgg gatggcgatt tttatcatca ttatgttggc aacaggaacg
    17281 cttgaaagaa atggtctgaa agaatcagcg gcttccttga tcaagcgatt caagaaagta
    17341 tctgctggta tgattgtaga tatttatggg atctttcgaa tgatctttgc ggcttttaac
    17401 gtgagttttg gtggagttgc tggatttgtt cgtccaatca ttttaccgat ggcattgggg
    17461 accatcgaat caaaagggtt agaagtccat cctaagcatg aagaagaact aaaaggtatg
    17521 gcttcagcta tggaaaatgt ctgctggttc tttggccaag tactattcat cggaggagca
    17581 ggggcgttgc tagtccaatc aacactgaaa gatctaggat atgacgtaac attgggacag
    17641 cttgctttag tggaagttcc agtggctatc acggctttag ttgtggcaag tgtctacttc
    17701 tatcttaaag aaaagaaatt agcgaagaaa tattatccag gaaaggggca ataagatgga
    17761 cttttttaca aatcctgata ttttattagg cgataaatta ctggagatcc tttatatcgt
    17821 gatgggactc gtattgattt acactggagt aagaaattta acagataaaa ccaatcctca
    17881 tcgttatggg tcagcctttt tctggacggt cttaggggta gttattgcag gaggaagatg
    17941 gcttccggca atcgtgaacg gtggcttgat ctttgcgatg accataccag caattgctaa
    18001 gaaagtcagc aaaggagaaa gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga
    18061 caaactagga atgaaaattt ttattccagc tttgagtatc ggtgtctttg cgatcttgtt
    18121 tgctctattt accaatttag gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat
    18181 gctaatcttg atgttctttt ctcgagataa tcgaccaagt acatttttag atgatgcagc
    18241 agatatgctg ggcacagtag ggccgttaag catgttgcca atgcttttag cttctctagg
    18301 tgcggtattt acaagcgctg gagttgggac agtgatttcc aatgcagtag gtacgatcgt
    18361 tccagaagga aatgtgaaca tcggcatcat catttacgct cttggcatgg tgatatttac
    18421 ggtaatcatg gggaatgcat ttgcggccat caccgtgatg acggttggga tcggtgcacc
    18481 attcgtattt agtcatggag cgaatccagc gttaatagga atggttgcat tgacttgcgg
    18541 tttttgtggg accctgctga caccgatggc tgctaacttc aatatcgtgc ctgtagcgat
    18601 gctggagatg aaagacaaat acggagtgat caaaaaccag ttgtttattg cgttatttat
    18661 gctagttttc caaattgctt atatgattct atttaaataa tagaagagag gggttgtgac
    18721 agaataattg tcgcaacctc ttctaacatt gtagtggaac actatttttt tataagaata
    18781 tattaagttt gatctgttat gttatttaaa ctattgtttc ctttatttac gctgttcaag
    18841 gaaaatggta tgataaaaga taaccatatg ttaaatcaat cggacggagg aacaacagat
    18901 gaaagtacta gtaacaggtt ttgatccatt tggtggggac aaagtgaatc cagcctatga
    18961 agcagtaaaa aaaatgccag atgaaatcag cggtgctgaa atcatcaaag tggaagtacc
    19021 gaccgtattc gaaaaaagca gtcaagtagt caaagaagcc atccaacagc accaaccaga
    19081 tgtcgtgatc tgtgtcggac aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc
    19141 aatcaatcta gcagaagcaa gaatccctga taatgaagga aatcagccat tcgatacggc
    19201 tttagaagaa aacggtccag ccgcttactt tacctcattg ccaatcaaag caatgacaaa
    19261 aaatgtacaa gatcatggtc taccagcgta catctcttat acagcaggaa catttgtctg
    19321 caatgatatc atgtatcgtt tgcttcattt gatcgaaaca caattcccgg caattcgagg
    19381 tgggttcatc cacgtgccat tctcacccga tcaagtgatc gatcgtccag tgggtacgcc
    19441 ttctatgtct ttagaagaca ttgcttcttc tttgacttat gcagttgaag cagcagtaga
    19501 aaataaagaa gatatttccg gaaatgccgg aacaactcac taatttagca agaaacactg
    19561 gctggttgct aacgttatgt tagtagccag ctttttgtaa ttagaaagga aagaaacagc
    19621 cgtggaagaa tggcaatccg tctttgaaga atggtttcca aaagaaatca gcaaaagcta
    19681 tcccatcaaa atttctaaac aatacacaag cagtcaacgt tgggagatct atgcaaaatt
    19741 aacgaaaaaa cagcgggaac tagtcgataa gcaccgccga tatctaatca gctctcgttt
    19801 tatggaagaa cattatttag ctgcaacaga ttgggtgttt tctgacttca agatcaatcc
    19861 gttttttcga acaaaacgaa gccagcaaaa attgtattgt gagtgtggta gagaattaaa
    19921 agtgcaatac atcgttaaat cgccaaaaac cggaaagatc ctaaaactag gcatcaacca
    19981 ctttgccgat cacctgcatg tctctccgac agtggcagct tcgatccatc agggaatgac
    20041 aaaagttgat ctggccttag acgaattatt atggcttaag cagaagaata tcgattttcc
    20101 tgaaggacta tggcaaaaat actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc
    20161 atatttaccg gatataaaat tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc
    20221 gatttacata gcggattatc aagcattgga aaatgaaatc aagaaaataa gtgaacacat
    20281 caatgggcag tcaaaaaagc gacagataaa aaaagaacta tttgacgact ttgcagaaga
    20341 attagtaaag gatgtagaag aattcttgat caactatcgc gcatttttac gtaaagattg
    20401 gcaatccatc gtatatgaag aagtgccagt acatcctaat gcttattttg aaacatttat
    20461 ttccgtgttg agaaagacaa aacgtcaacg aacaccagaa gtcactgctc agatggaata
    20521 ttttgcgaaa aaccaacgat tcatccaacc aaaaatctat ctatttattt ggaagcaata
    20581 ttgtcgctat ggttttacag aagggttctt cgacagcatt cctcggatag tcagaaatgg
    20641 ttttttaaaa gtattgcgta aagaacgaga agctatacaa tctgctgata agaaagaccg
    20701 tacagtctca aaagaaaaat ggcaactagt agtgaaagat atccaatctg gaaatgtgca
    20761 agagacaatc gacaaatgga aaggaaaaca ttatcgtttt actgaagctc agaaacaagc
    20821 gttggaatat tatcagaagt tagaagaatc actgcgtttc aatgatgagg caagaaagta
    20881 tctgaaggaa ttactataaa aaa
//
LOCUS       DACEMO010000028        20780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEMO010000028 DACEMO010000000
VERSION     DACEMO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.028"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(6..1207)
                     /locus_tag="IW728_000810"
                     /pseudo
     CDS             complement(6..1207)
                     /locus_tag="IW728_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1324..1839
                     /locus_tag="IW728_000811"
     CDS             1324..1839
                     /locus_tag="IW728_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1024188.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2038..2484)
                     /locus_tag="IW728_000812"
     CDS             complement(2038..2484)
                     /locus_tag="IW728_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1024189.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2531..4009)
                     /gene="msr"
                     /locus_tag="IW728_000813"
     CDS             complement(2531..4009)
                     /gene="msr"
                     /locus_tag="IW728_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1024190.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4363..4992)
                     /locus_tag="IW728_000814"
     CDS             complement(4363..4992)
                     /locus_tag="IW728_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1024191.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(4976..5440)
                     /locus_tag="IW728_000815"
     CDS             complement(4976..5440)
                     /locus_tag="IW728_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1024192.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5927..7195)
                     /locus_tag="IW728_000816"
     CDS             complement(5927..7195)
                     /locus_tag="IW728_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1024193.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7426..8163)
                     /locus_tag="IW728_000817"
     CDS             complement(7426..8163)
                     /locus_tag="IW728_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1024194.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8230..8799)
                     /locus_tag="IW728_000818"
     CDS             complement(8230..8799)
                     /locus_tag="IW728_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1024195.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8927..9799
                     /locus_tag="IW728_000819"
     CDS             8927..9799
                     /locus_tag="IW728_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1024196.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10141..11013
                     /locus_tag="IW728_000820"
     CDS             10141..11013
                     /locus_tag="IW728_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1024197.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11010..12968
                     /locus_tag="IW728_000821"
     CDS             11010..12968
                     /locus_tag="IW728_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1024198.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13219..14070)
                     /locus_tag="IW728_000822"
     CDS             complement(13219..14070)
                     /locus_tag="IW728_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1024199.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14193..14660
                     /locus_tag="IW728_000823"
     CDS             14193..14660
                     /locus_tag="IW728_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1024200.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14923..15405)
                     /locus_tag="IW728_000824"
     CDS             complement(14923..15405)
                     /locus_tag="IW728_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1024201.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15505..16368)
                     /locus_tag="IW728_000825"
     CDS             complement(15505..16368)
                     /locus_tag="IW728_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1024202.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            16966..17814
                     /locus_tag="IW728_000826"
     CDS             16966..17814
                     /locus_tag="IW728_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024203.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(17985..18326)
                     /locus_tag="IW728_000827"
     CDS             complement(17985..18326)
                     /locus_tag="IW728_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1024204.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            18412..19119
                     /locus_tag="IW728_000828"
     CDS             18412..19119
                     /locus_tag="IW728_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1024205.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            19106..19429
                     /locus_tag="IW728_000829"
     CDS             19106..19429
                     /locus_tag="IW728_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1024206.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(19518..20555)
                     /locus_tag="IW728_000830"
     CDS             complement(19518..20555)
                     /locus_tag="IW728_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1024207.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT         6193 a         4298 c         3689 g         6600 t
ORIGIN      
        1 atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat acaacataca
       61 ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat atgttcacca
      121 atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa actatagctg
      181 gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt caacagtgta
      241 ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag gactggcata
      301 gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc tgctaaaatt
      361 gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga agcaatagcc
      421 agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata gccaatcaat
      481 aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa tgcgattagt
      541 ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag gcctactttt
      601 ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc gcaaaacatc
      661 aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac cccaccgact
      721 agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat tgctttttct
      781 gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa taaagcattc
      841 ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga taatccgcca
      901 agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttc aaaccgatcc
      961 gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg taactagtaa
     1021 ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg
     1081 ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca actagcgctg
     1141 aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga ttctccaata
     1201 tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt aaatatgcac
     1261 atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag aggtaggatt
     1321 tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc acgttctggg
     1381 tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca gatctataca
     1441 acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga cgagcactct
     1501 caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga atggatcaaa
     1561 gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta tgctttttcg
     1621 acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca gctgaacaaa
     1681 atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa acaagaattt
     1741 ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact ccaatggtgt
     1801 cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag aacctttcta
     1861 gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt ggaaaagaaa
     1921 aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc tagaactgca
     1981 ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag tgttccatca
     2041 ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga tgatcaactg
     2101 caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag agaataattg
     2161 atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca ccattcctaa
     2221 aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat ttactggtag
     2281 tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa tgaaagccac
     2341 cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt gatgcagcca
     2401 taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg taaaaattat
     2461 tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca cgaaaatcat
     2521 cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct tttatttcat
     2581 aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc
     2641 ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca ataaaattag
     2701 tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca atcgtcagac
     2761 gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc tgagcaaacc
     2821 ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa
     2881 aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat acttgaaagc
     2941 ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct aataaggttg
     3001 tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa aactgaaaat
     3061 caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt cccatgatgg
     3121 gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt ttcatctgct
     3181 gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc gcattctttt
     3241 ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc ggtctgattt
     3301 gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt ttcttttctt
     3361 ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct cgtagctgag
     3421 tctttttctc caattccttt tgagtgagat aggcactata attccccgaa aactctcgga
     3481 tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa tagcgatcat
     3541 gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt cgatcaatac
     3601 tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca taagaagaaa
     3661 gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc tctgaagttt
     3721 cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt gcttcagcga
     3781 gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc ccatgatcgg
     3841 gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca ttttctccta
     3901 tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt tctagctggt
     3961 tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata tgttttacac
     4021 tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa atctgtttgc
     4081 aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact aaatcaccaa
     4141 atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata ctccatccat
     4201 gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa agttcgaaac
     4261 gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta ccacacacga
     4321 ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga acgtagtgat
     4381 cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc ctattgataa
     4441 aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca actgatctgc
     4501 ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta acatgaattg
     4561 catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc ttgaaaaact
     4621 cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg gcatcttccc
     4681 tgattcgtaa gcttggttat agatttcttc gccatattcg taacattgca tatcgccaaa
     4741 aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg
     4801 caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt gcatattgac
     4861 agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt gtgggatttt
     4921 gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg aaaaatcatt
     4981 tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa agttgggtat
     5041 tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa gccggggata
     5101 gcacggaacc tgtatcttta tggtccattt cacaatgctc gattttcttc tgggcttttt
     5161 ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc gaaacatagc
     5221 catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc tgcaactttt
     5281 cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg atctcgtatc
     5341 tagtaagact tataccgata ttttcttcaa agatctggac gattttttga tcggctaatt
     5401 tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc tttctaataa
     5461 ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat
     5521 cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga aattttttgt
     5581 agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt attgattctt
     5641 atcaaactat agaagatagc tcttctgcag tagaaaattt tacaccgcca aaaatttccg
     5701 aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat gcttaaacag
     5761 taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa
     5821 acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg
     5881 gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa taaccataaa
     5941 atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc ttagcactca
     6001 aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa attggcttgg
     6061 aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt aaagagttga
     6121 tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat aaacggtaga
     6181 caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag cctacagttg
     6241 gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta tgacggttat
     6301 acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc agattatagg
     6361 tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa cgacctgtta
     6421 gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg gaatagtagt
     6481 aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc aatgattctt
     6541 tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca ttcacatatt
     6601 ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca aatagattgt
     6661 aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg gcttgagcaa
     6721 tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt gaggcaatac
     6781 tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca tagccagtat
     6841 agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc ccatctgtca
     6901 attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct gatgtactgt
     6961 cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa attgtatttt
     7021 ctgagacagt agctgactgc aaagtactct ctgtatcgtt ttcaattcct gtttctgaag
     7081 aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact gttgtgttgg
     7141 caactgctag atttcctaaa agaataacgc taaataataa attctttgct ttcatctttt
     7201 ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa gtaaaagcaa
     7261 gtcaatgtcc atatttaaat aacatttgtt tctttttgat aaatttgctt tttctacata
     7321 aaaagacgta aatgaagaat attcttctcc attcacgcct taaattgatg gtcgtttgaa
     7381 taactattta acaagtaatt ctctttggat attatccgtt atggattata cttcctgtgt
     7441 cgtcggacgg ataatcatgt tactgacaga catccggtct ggcgtttcta tcgcaaatgc
     7501 tacagcagaa gctatatctt cggaggtcaa tccccattct ttttgcgctt catgaagttc
     7561 aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag ctcctggaga
     7621 gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt ccatgattgc
     7681 acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa catgtcccgc
     7741 cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg gaagtacggc
     7801 ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc attcatcacg
     7861 gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca atacatcgat
     7921 tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa aatctcttac
     7981 atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg cttttaggcg
     8041 atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata gcttcgttgt
     8101 tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg ataaatcatt
     8161 cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc tcttatttca
     8221 tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga ttcctgcaac
     8281 gatggtattg tctgggacat ctttcgtaac agttgcattc gctgcaacaa tggcattctc
     8341 gccaatcgtt acgcctggca gaatagttgc tcctgctccg atccaagcat ttttcttgat
     8401 gtggattggc tttactgaca agcctcttcg ttcttttgga tcggttagat ggttcaccgt
     8461 gatgaggttg acacgaggac caatcaatac tgaatcttct aaacagatgc cacctaagtc
     8521 aacgaacatc acttgacgat tgataaagac atttttccct atactaatat gtttgccgaa
     8581 atctgtataa aaaggcagca gaacagtcac cgtatcatct actttgtcac taatgatatt
     8641 ttctagccgt ttgcggatat ctgctggttc atggtaccca gtattcagcg ctgctaaagg
     8701 ctcttcgttt tcagcttgga tctgatggat cacttcaaag agcggatccg ttactcgaat
     8761 ctctttattg acgatttgtt tttcgatgag gcttgtcatt ttgctccctc cttttatcct
     8821 tactttataa cggacggctt atcatgtcta atacttataa aacatactta actattcctt
     8881 ttaactatac ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg atattcgtat
     8941 actgacgtat tttttagcag tagctagaga aaaaaatatc agccgagcag caaaatctct
     9001 gcatatcacg caacctactc tctcaaaaca actgaaagat ttagaagaag aacttggtgt
     9061 gacacttttc actcggggct cccgagagat acagttgacg gattctggtc gatatcttta
     9121 ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt taacaaatga
     9181 cgcaatcatc tctggagaaa tctacattgg cggaggcgaa actaaagcaa tctctttcat
     9241 ttctgatagc cttcattcgt tgatcaaggc gcatcctgat atccagtttc atttatatag
     9301 cgggaatgca gatgatatca tagacaaact ggatagcgga ttacttgatt ttggactcgt
     9361 aatcgaaccg acaaacaaac aaaaatacca gtcatttcga ctgccagccc aagatcgttg
     9421 gggacttttg atacatcgaa atcatccttt agcaaagcaa accgtcatcc atccgcagga
     9481 actgaaaact gttcctttga tcatttcccg gcaaacgtca gtggacagtc aactagctga
     9541 atggctaggt ttcagcatag atcagttatc gatcgtcgga acatataact tattgtacaa
     9601 tgcttctttg atggtcgata acgggacttt tggtgcatta gctctagatg ggatcatcaa
     9661 tacttatggg acggaaaaaa tattcatccc actcgatccg ccattacaag cttcactgaa
     9721 tctcatttgg aaaaaagacc agcctctttc tcctgctgct cgtaaatttt tagatgtcct
     9781 aacggagaaa tacagttaag tagagttttt ttccttcgct ttcagctttt taacaacgaa
     9841 taagataagc aaaagatcca acgattaaat aacataagtt tcttaattta tttcttcctt
     9901 ttatttctga taattccagt aaattactat aattataaaa aaagaattga caaaacataa
     9961 ctgtctatat aaaattgatt acgttaacaa taattcgttt catgtgacta gatcatacta
    10021 ggcatgaaaa atctctgagg aacatggttt ccctaggagg tttttcgtgt ctatttttgt
    10081 ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa gggtgcgcgc
    10141 atgaaaatca aaaagatctt taatcaaaat gcagtattgg tagatgacgg tggacaggaa
    10201 aaagtagcta ttggaaaagg gattggattt gacagaaaaa gaaatgattt gatttttgat
    10261 cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt gcaaaattta
    10321 cttaatcaga ttgatgaacg ctttttattt gcagcagaaa cgatcattga tcatgcggaa
    10381 actgttttga tggaaaagtt gaatgaacat gtattgatcg ctttgaccga ccacattgct
    10441 ttttctgcgg agaacattaa taacggtatc gtcatccgca acaaactttt aagagaaatc
    10501 gaagtgcttt acagtgatga attcagtatt gctcaatggg cagtagagta tctgacaaaa
    10561 gagcttggga ttccatatac ttatgacgaa gcaggatata tcgcaatcca tattcacagc
    10621 ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg aagtgacgat tgtatctgac
    10681 atcatccatt tagtggaatc agaattggaa acagatatcc attcagaacc attttcactg
    10741 aattattcaa gactggtgaa tcatttacga ctgcttctac aacgaaccca tgcgcaacaa
    10801 tatgctgttt tagacacgga aatcgtagaa atggtcaaac gaaaataccc agaaagctat
    10861 aagatttcta aaaaaatccg tgtgttgctg accaaagaat accagctcgc gatcacgaaa
    10921 gaagaactgg gttacttggc catccatatc gagcgtttaa gaagcgcaat cgtacaaaca
    10981 aatgttaaca atcatgagga ggaaaaaaat tgaaagcata tctacaacga atgggacgtt
    11041 cccttcaact tccagttgcg gtcttacctg ctgctgcttt actagtaggg atcggaaact
    11101 ggtgggcatc ttatagtaac gacattgtcg ctcatttttt acaagctggc ggtaacgcag
    11161 ttttaggtca attacctttg ctgtttgccg ttgggttagc actagggatg tcagtggaca
    11221 aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga agttccagta aatgttttaa
    11281 aaactgattc agtagctact ttattgaata tcaaaccaga atcagttgat ccatcttttg
    11341 ctcagatcag taacgtattt attggtatta ttgccggatt gattgcggca gctctttata
    11401 atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc aagcgtctag
    11461 tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc agtgctactg ctcgtatggc
    11521 ctccggttta ctctgcttta gtttcttttg ctaaattcat tttgagttta ggctggggtg
    11581 gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc tacaggattg catcaagcaa
    11641 tgaactccgt gttctggttt gacgtagcag gaatcaatga tattggtaaa tttttggctt
    11701 ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg atttttccca atcatgatgt
    11761 tcgggcttcc agcaggagca tatgctattt acagaaatgc acgtccagaa aacaaagcaa
    11821 aaacagcttc cttgatgcta gcagcagctt ttgcttcatt ctttactggt gtgacagaac
    11881 cacttgaatt ttcatttatg ttcgttgctt ggccattata tgtcttgcat gcaattttca
    11941 ctgggatttc tttagcagtg agtgcattct tccattggac agcaggattt gcctttagtg
    12001 ccggatttgt cgattacttc ctatctttga aaaatccgat tgcgaatcag ccattgatgt
    12061 tgatcgtcca aggattagta acagcagtga tttactactt tggctttaac tttgctatca
    12121 aaaaattcca tctgatgact cctggccgcg aggaagcaga tctttcagat gacgatactg
    12181 caacgactaa ttcagacaat aaatatgcgg cacaagcaag caaaatctac gctgcattag
    12241 gcggagccga caatgtaact tcgatcgata actgtactac acgccttcgt ttgcaagtca
    12301 aagatacagg cctgatcgat caaaataaaa tcaaagccac tggcgtacct ggtatgaaga
    12361 tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga agtacaattc gttgcagatg
    12421 agatggcaaa actacatggt ggtgctgcag cacgacctgc acaaacaaac accgtggtta
    12481 aaacagaaac atttgccggt gaaacgatcg aacaagatat ttacgctatc gcaaatggga
    12541 aactgattcc aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg atgggtgacg
    12601 gctatgctgt attaccagaa aatggagaaa tcttctctcc aatcgctggt acgatcacaa
    12661 acattttccc aacgaaacat gctgtcggaa tccaaacaga tgctggtatc gaagtcttgc
    12721 ttcatatggg catcaacact gttgacttaa aaggcgaacc attcacttta tatgtagaag
    12781 aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc gctattcaat
    12841 ctgcaggcaa aaacacggat atggtcgttg tattcacaaa tggcgacaaa gtccaaagct
    12901 tagaaatcga acccgcacgt gatgtaaaag caaatgataa aatcggctct gtttccaaca
    12961 aagcctgagc catcgtcttc cccagacttt tttatcgata aacaaacagg agctgagaca
    13021 aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc aactccgcgg
    13081 taataagtcc agctaaacga aacatatgaa gagctatttt ttatttgact cttcttatta
    13141 cttgatgcag aacagctttt tcacaaaatt tttcttcata ctcaaaacta atcggctttt
    13201 tcacaacctc tttatacatt attcctgatc caatatttct tttaatgctg cttcaagaga
    13261 agttaatggt tttcccaata ctttttctaa atcatcagag gaaatagcta actgatcctt
    13321 tttaatatca tgttggatcg ctaaaaacat gtcagctgct tcttgaggga atccggcttc
    13381 ttttagattt tcgacaaacg ctgcatcact tgcttcaata atttctaact ctttacctgt
    13441 cgcacgctct aatgccttgg ctaattcttc atagtcagtt agattgccgg ataattctaa
    13501 gatttctgga gagtcacttt ctactagtac ttttgctgca gcttctgcat attcacgttt
    13561 caatgcccaa cctgtttttc catttcctgc agcataaacg aattgaccag tttttaaagc
    13621 ctgcccaatg ataggtaatt cattctctac ataccaatta ttccgtaaaa atgtatggga
    13681 aatacctgaa tccttaatca ttttttcagt gaattggtga tctggcgcta atacactgat
    13741 cgagtgatcg gcatctgcaa aacttgtata agcgatataa gataccccag ctgcttgtgc
    13801 tgccttcact acattcttat gttcggcttg acgatttcct ggaacgccag agacaaaaag
    13861 caatcgatct attcctttca acgcttcttt cattgagtct aaatcatcat aatcaccaat
    13921 acgtatatta aatccagctt ctttcagtgc ttctcctttt tcttcactac gtactagtac
    13981 atagatttca ttttgtggaa ccatcttttt taagaattcc agcgcataac caccaaaacc
    14041 tccagttgct cctgttacta aataatgcat atttgccact ccctattctt tttatttgtt
    14101 aagatttaca gctgtacatt tataaattac acctttatca taaagagtgt ggtaagataa
    14161 gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa tttagtgatg
    14221 caatccatgt acttgcctat attgaaatgt ttcaagatac ccgatcactt tctagtgaga
    14281 tgattgccag tagtatcgaa acaaatgcag caaatgtacg taaaatcatg agtcagctca
    14341 aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca gccttccctt gccaaaagtc
    14401 cagatcagat ttctttacta gatgtctatc gcagcatcga aggtaataca cagcttatcc
    14461 aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa gaagctttag
    14521 aagaaagcta cgctcggctg cagaaaaagg cagaagaaga aatggctaag atcacacttg
    14581 atagtctgat acataaaata gccgttttgg aaaaagaaaa aagaccagaa aacacaggcc
    14641 tcgtagagag atttttataa ttctctctac tgtgcctgca tttttctgat cggaatcgtc
    14701 tacataaatt cttttattct ccgggggaca aaagccttgt catgcccctt tttcctctct
    14761 aatgctcagg tcaaagcgct gaagtcatga cgtcttgata aacggtgttc aagtagctga
    14821 ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc tatttcccat ttttcctctc
    14881 taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt tattgactac
    14941 cggtatccag atttctccca tatatttccc gttggtaact cccatttcga ctacagcatt
    15001 tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa aggccgtatt
    15061 cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgcttacaa caagatacgt
    15121 tccttttggg aatgggatga ttcgcgtagt gtttggaaca tcctgactcg tttgaacacc
    15181 gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat aatgattttc
    15241 agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct caaatgcttg
    15301 tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca caccaatacc
    15361 cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc ccaccctttc
    15421 tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta cgctcttttt
    15481 tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc aaatctcact
    15541 gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag cttcgactaa
    15601 cccataatct gaagaaggaa gccattctgc atagattttc ccccaagtat cttgtaaagt
    15661 ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta tagcaagcat
    15721 ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt gcatcaaact
    15781 gcccttttct tccaagcggt ctccatcaaa tccataagaa acgttgatta cttgatttgg
    15841 atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg tctgcgctaa
    15901 cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg ccacacctgc
    15961 cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc ctctgacatc
    16021 aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctactga tttctgaagg
    16081 cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt catatccata
    16141 tctaaacgcc gtttctgtca ctcttgatcc atctagaaga tcgcgattag ccaaagccaa
    16201 tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa agatgcgttt
    16261 taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa gtccttctgt
    16321 caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat ttatcttcct
    16381 ccttgattgc ctttttatca tattatcaaa gaaagtcaat tacttgatga atcaggaacc
    16441 aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa gcagcgtttc
    16501 gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa ataaattttg
    16561 gggaagacag attctaagta aacaaagcgg taaaaatatc ttttattccc ttagttaatc
    16621 cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag aacgctgctt
    16681 ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta tttatttcta
    16741 acaaaatcac ttcatttttg tcagataaat aaaaaacaaa aaaagttcca tctttaaaac
    16801 acgctattct tttacttttt ctcacattct ctaagaaaag aaaaggagaa tgaatttttg
    16861 acaattcgag caaaagtgat tatcaaatat gtaaaatcag ctactttttt cttaaacgtt
    16921 tctttatact aaatataaat aagtgtttca tgaaaggaga actagatgaa taattggctt
    16981 gagtattttc ctgaaaacgt tttggaacgc ggttacagct atcatctgca cggttttgtc
    17041 cgtcatttga actatacaag caagtacttg tccgcaacag tttctggcac ggaagattat
    17101 aaggtagtga tcacttggga tgaaaagacc aacatgactt gtgattgtct atatgccata
    17161 gaaggaaaaa aatgcaaaca catggctgct gtcttattcg cttacgaaga acgtccaata
    17221 aaaaaatcca actactcact ttccgaactc tcgtcccttg tttcctccgc tagttcctct
    17281 cttgtacggg agcttttaac agagattctt atagaacatc cgcactttat cgaacgcttc
    17341 aaagttaaga tgccatttca tgcaataaat tattcagata aattaacaac tatcatccat
    17401 aaatacgatc atattatcaa gaaaaacaaa aacaggaaaa cagcgaagtt catcatggag
    17461 atgcgaaagt tcatccaaga agcagttgaa tcgcttattc agcaaaatgc ctatcttccc
    17521 gcattcgaat tgatcaatga agtgattgct actctcgaaa cattctattg ggagcctgag
    17581 gatgaacgaa cccttcttct gatagaggac tgttattatt tatggaaaga attattagca
    17641 gaagcacctc atgcagaaaa aaggcaaatg ttctcttggt tcgtctgcca agtcgatcat
    17701 actgacgcct cttattcaaa acgctactct ataaaaatcc tgaaagaaga ctttcgggaa
    17761 aaagaatttt ctaaccaaaa gaaaaaaata gataaaaaac taaagaaacg gtaaagaaag
    17821 acgatttcaa tgaaaaatag caagtaaacg ataacaacca tcctaaatcc acaagaaata
    17881 cagcagacct tgctttaaaa acacgaatca tgcaaaggat gaagtaaaga agacttaacg
    17941 cgtacccaat attagtcatt tgactattag gtacgcgttt tgttttataa gtcatgacaa
    18001 atttccttcg ttgctggata gattttccgg cattgttgat agattttatg atacttttct
    18061 acattttgta agagaggttc cgctgcatct gtatattgga taaaattctg acaacaactt
    18121 tcaggtgtgt cgaaccaccc taaccctagt gcagcaatca ttgttgcgcc taatgcaggt
    18181 ccttcctcca tacttaaaca atagaccgtt gcatcgaaaa tatcagcttg gatttgtagc
    18241 catgcaagat ttttcgcgcc tcctcccacc gaaacaatac gtgtaaatct gcgacccgct
    18301 agttctttac gtttcaatcc caccattgac aaatgctcat taaaaattta gaatattcac
    18361 aagtttaacg acaattatga aaatttatgt cgtgctaaag gagttttgtt attggatgaa
    18421 aagttagata taaaaactgc gatcaaagac acgttaccta ccgttttcgg ttatatcggt
    18481 attggacttg catttggtat cgttgggaaa gctgccggat ttcatccact agtcgtcacg
    18541 ttgatgtccc tgctggtcta tgctggttct gcccaattta tcacagtcag catgcttgct
    18601 agtcacagcc cattgctttc catcgttttc tcgacctttc tagtcaattc ccgaatgatt
    18661 ctgatgagta tgacgattgc tccttatttc aagaaaaatc ggctgcttca aaatctgctg
    18721 attggcacat tgctgacaga tgaaagtttc gcactgggaa tgaataaatt gaactatact
    18781 ggacaaaagt taaactttcg ttggatgaat acagctaatt ggatttctta tttgacgtgg
    18841 gtgggttctt ctcttgttgg cgcactttta gggaatttta ttactgatcc gaaaaagttt
    18901 ggtttggatt tcgccatcgt cgctatgttt atcgggttac tttatcttca agtcatttct
    18961 gatagaaaca cctctaaacg attacagttg atcctgattg gtctcacttt gattctggta
    19021 tatgtcggac tgatttttat cccaagcaac ttagtgattg tagttgtaac actgattggg
    19081 tgcggattgg gggtgtggat caaacatgcc ttcttttgag tttacattgc tgactatttt
    19141 aggttgtact ttagcaactt ggctttctag ggtcctacca tttgtcttac tgaaaaaatt
    19201 cgatttaccg caaccgttgc ttgagtattt aagttttgtc ccgatcgtca ttatgtctgc
    19261 tttatggttc agcagtctat ttacccaaaa tatcggacat ctgccgcaga tcaatctcga
    19321 aaatgctttg gcttcagttc ctacactttt agctgctatt ctttcaaaaa gcttattggt
    19381 gatcgtcttg gcaggaattc tttccctttc gttgattcgc ttattataaa taaaaaaaca
    19441 agagcttatg gcaaattact gtcatgcgct cttgttttta tcaaagtcaa ataagaaaaa
    19501 aatcggcgta ttcttgctta tccgttgaac ggaccactaa caacgacttt tcctaacata
    19561 tgtcctgttt ctacgtcttt ggttgcttgt ttcaagttct tcgcattcat cccatctgaa
    19621 tagaccttgc tcaatgtact gcggataatc ccttcatttg ctaaagcagc aatgtgcgct
    19681 aaagcttctc cttgcgtttc gatttcatag ttatagtctg ttttagcgaa catatactcc
    19741 cagtcgaagc tgatagattg gtttttccaa acacctatat ctaacggctc tttgatccct
    19801 acgatcgttc ccacatgtcc aaatggtcgg acaaggtgct tgatttgatc aaagtagcgg
    19861 gtaatatcga ataatacagc gacatactct acttgatcga ttcctaattc acgaaaactt
    19921 ttacttaagt cgtgatgata atcgattgga tagtctacac ctgttttttt cagccattca
    19981 aaattcttcg gacttgcagt cgcgtagact tctaggccag cccaatgcgc tagttgattc
    20041 aaaatcgaac cgacgccccc ggatccgttg atcactaaga tttttttccc gcgattggca
    20101 ttttcttctg gagttagacc aaatttttca aataacaatt cataagcggt caatgaggtc
    20161 aacggcaagg ctgcagcttc ttcatctgaa agattttttg gtgcaagagc gacaatacgt
    20221 tcgtcaacta gttggtattc ttgattgctt cctgctcttg ttgttgtacc tgcgtaaaaa
    20281 acgcgatcgc cgacagaaaa tttttgcact tcgtcaccta cagcaacaac tttcccgact
    20341 ccatcaaaac ctaagatacg taaagcattt cttatcccgt tatcttggcg aagtttcgta
    20401 tcgacaggat taacagatac tgcgttgact tttaccagaa gatcccgtga actgggtaca
    20461 ggtatctgtt ctttttcatc aataaagctt tttggatcat cgatcggcaa cccttcataa
    20521 aaaccgacta ccttcattaa ttccttttct gacatacgaa ttcctcctta gcttcatcaa
    20581 gttggagcat ccatacagtg ttcatttcaa tatatatagt atgctatttg ttccaccttt
    20641 tttattcatc ataattattc tcttacttaa agtaaactat ttgcactttt ttggttaaaa
    20701 aagaggttgc gacaagactt ttgtcacaac ctcttataaa cggtgttctc ctaccaactc
    20761 ttcggtcaca agtcaaaata
//
LOCUS       DACEMO010000029        19574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEMO010000029 DACEMO010000000
VERSION     DACEMO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.029"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..400)
                     /locus_tag="IW728_000831"
     CDS             complement(<1..400)
                     /locus_tag="IW728_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024208.1"
                     /translation="MCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIAEFIGSFRG
                     NVTTQPRRSWSIKLLNNSGELKAFHYEFLII"
     gene            complement(967..1902)
                     /gene="whiA"
                     /locus_tag="IW728_000832"
     CDS             complement(967..1902)
                     /gene="whiA"
                     /locus_tag="IW728_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1024209.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1936..2934)
                     /locus_tag="IW728_000833"
     CDS             complement(1936..2934)
                     /locus_tag="IW728_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1024210.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2931..3815)
                     /gene="rapZ"
                     /locus_tag="IW728_000834"
     CDS             complement(2931..3815)
                     /gene="rapZ"
                     /locus_tag="IW728_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1024211.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3950..4681)
                     /locus_tag="IW728_000835"
     CDS             complement(3950..4681)
                     /locus_tag="IW728_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1024212.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(4685..5950)
                     /locus_tag="IW728_000836"
     CDS             complement(4685..5950)
                     /locus_tag="IW728_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1024213.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(6213..9029)
                     /gene="uvrA"
                     /locus_tag="IW728_000837"
     CDS             complement(6213..9029)
                     /gene="uvrA"
                     /locus_tag="IW728_000837"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1024214.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(9039..11033)
                     /gene="uvrB"
                     /locus_tag="IW728_000838"
     CDS             complement(9039..11033)
                     /gene="uvrB"
                     /locus_tag="IW728_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1024215.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11292..12794
                     /locus_tag="IW728_000839"
     CDS             11292..12794
                     /locus_tag="IW728_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1024216.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            12796..13533
                     /locus_tag="IW728_000840"
     CDS             12796..13533
                     /locus_tag="IW728_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024217.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(13578..13751)
                     /locus_tag="IW728_000841"
     CDS             complement(13578..13751)
                     /locus_tag="IW728_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1024218.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            13727..14392
                     /locus_tag="IW728_000842"
     CDS             13727..14392
                     /locus_tag="IW728_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ1024219.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(14474..15640)
                     /gene="dnaJ"
                     /locus_tag="IW728_000843"
     CDS             complement(14474..15640)
                     /gene="dnaJ"
                     /locus_tag="IW728_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1024220.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(15791..17620)
                     /gene="dnaK"
                     /locus_tag="IW728_000844"
     CDS             complement(15791..17620)
                     /gene="dnaK"
                     /locus_tag="IW728_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1024221.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(17671..18234)
                     /gene="grpE"
                     /locus_tag="IW728_000845"
     CDS             complement(17671..18234)
                     /gene="grpE"
                     /locus_tag="IW728_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1024222.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(18328..19371)
                     /gene="hrcA"
                     /locus_tag="IW728_000846"
     CDS             complement(18328..19371)
                     /gene="hrcA"
                     /locus_tag="IW728_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1024223.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
BASE COUNT         5484 a         4272 c         3388 g         6430 t
ORIGIN      
        1 ctattattaa gaattcataa tgaaatgctt tcaactctcc actattattt aaaagtttta
       61 tactccaact tcgtctaggt tgagttgtca cattccctct aaaacttcct ataaattctg
      121 caatcaccgt atcttcaatc aaaattcttt cctcatctat ttcatattca agactaaacg
      181 gaaattctcc atatgtaatc tctggcaatg gtggtgctgt agttctccaa ataaatacaa
      241 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
      301 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
      361 ctgcaatcat aatgatagta aaagttgtaa aaaagcacat tttttgccct ccctttataa
      421 acatcgattc tcaaattcta aaccaaatct tgatagttca agttcaggta taacgtatac
      481 caaccataca tcccaattac tatttatgaa cttatgatta tcactgaata aatcactcca
      541 tccccttgtt aaaacttcta cattaattac acctggttgt tttcgactag ctaatccaat
      601 gaacttatca accatcctta tccaccaacc acgagaatct tgaccagaag taagaaaaac
      661 agttctcaag gttgatcgag cagttccttc cagtttttaa ttaatctcca gttatagata
      721 aatacttgat cctatctata tcaggacttc tatattaagc aaaagtttat gtataaaact
      781 aaaatcttca ttcagttgtc tccccgcttt ttatacgatt gtattaaaaa acaatagaag
      841 cctttcaaca ttatgttact ccagtaaagt tggaattata gtttgacgga agaaaagcaa
      901 gtatagcttt taaaaaaaac tgatacattc gcatctcctg cttcatgtat cagttttttc
      961 tttggattaa actgcttctt cacgcagttt ttccgcaaat tcatttattt tgcgaattcg
     1021 gtgattaatt cccgattttg atattgcacc tgagggaatc atttccccta attctttcaa
     1081 gctgacctct ggatgttgaa gccgaagttc tgcgatttcc tgaagttttt caggaagggc
     1141 atgtagaccg acctttgctt cgatcaattc gatgttttcg atttgtttag aagccgcatc
     1201 aatcgtcttg ttcagatttg ctgtctcaca attgactaga cgattgaccg aatttctcat
     1261 atcccgtacg attcgaacat cttcaaatcg gagcattgag ttggttgcac caatcaatgt
     1321 taagaaatca gcaatttttt ctgctccttt taaatacgaa atatagccat tgcgccgttc
     1381 aagcgttcga gcatgtagac cgtaataatt taacatatcg caaatgtctt ggttatgctg
     1441 ttcataaata gaaaatattt ccaaatgata acgactcgtt tccggattat tgactgaacc
     1501 tgaggccata aaagcaccac gtaagtaaga acgcattttt tgcgaatttc ccataatctc
     1561 gtccgaaacg tgtccattga acatcatacc atccatgata tcaagatcca tcaacacatc
     1621 ttttgtgcct tgcttcaacc ggacgatata gacattgttc ttttttaatt tcattttttt
     1681 acgaacgagt agctcacttc gtacctgata atgatctttc aaaagagaat agatacgtct
     1741 ggcaattgca gcattttctg tttggacgtt caaaataaac tgctggttca caatacctaa
     1801 agagccgttc attcgcagca aagcagctaa ttccgctttc gcatgttctc gatgaacttc
     1861 caaaccagtt agttctttct tcacatctgc agcaaatgac atcctattct cctcctttct
     1921 ccaacaataa attgcttaat attttgaacc gaaaactaaa cggaacaatt cttctactac
     1981 tttgtctcca tcgtggaaga caccaccatc tttcaattct aaaaagtcgg tggaaatcac
     2041 tcgacatcct tcatttctca atccgttgaa gtcgtgtgcg acttgtacta gatactcatc
     2101 atagatctct gggtccatat aatcttcagg cactttttcc gtattgacca atactgtatc
     2161 gataaaaggc tgattcaaat gcttgtgtaa aacacgtata tgatcggcat ctgtgaaatg
     2221 ttccgtttcc cctttttggg tcatgatatt acagatatag acggtttcag cagttgtctt
     2281 gagcattgct tcgccgattt ctggtatcac taaatttgga aggatactgg tgaataaact
     2341 tccgggtccc aaaacgacca tgtcggcgtc cataattgct gaaacgactt ttcgagccgc
     2401 tttggcttca cttttatcat ttgtgttgca gacatacaca cggtcaatgg ttttacggtt
     2461 gatcgcgatt ttagattctc ctactgcagt cgttccgtct ttaaaaacag catgcaaggt
     2521 caaaggagtt tctgaagaag gataaacatg tccatctaca tgcatcattt ttgctaataa
     2581 ttgaatcgcc tcataagtgc ttccccgcat ctcggaaact gcggcaatga tcagattacc
     2641 gatcgtatga ttggctaaaa actgatcttc ctttttgaaa cggtattgga aaatatcttc
     2701 atagaattgt ggcatatccg ataacgccac taagacattt ctcaagtcac caggaggagc
     2761 catagcgatc gattctctga ttgcaccgct gcttccacca tcatctgcaa cagtcactac
     2821 tgctgtgata tctgctcctt gatttcgtaa acttttcagg ataactggca gaccagtgcc
     2881 accgccgacc actacgatct ttggttttct tatccggtag gttttcattt tcacgaccgg
     2941 ttcaccgtct ctttcctttt atccttgtcc cgatgtgtca cattgacttt atagtttttc
     3001 cctaaagcat ttgccaaacg ttcagtcaat gccacagaac ggtgttgacc gcctgtacag
     3061 ccgattgcaa ctgtcaagct gctttttcct tctttaatgt atccaggcaa tacagtttcc
     3121 agaagatcca aaaatttatg gtagaatgat tgcgtttcat caaaggacat cacataatca
     3181 tagactggct gatcttttcc ggtcaatggt cttagttccg ggatataatg agggttgggc
     3241 aaaaaacgca catccatcac aatatccgaa tcgatcggca acccgtattt aaaaccaaat
     3301 gatacaaatt cgatccgaaa tccttggtct ccctttgatt tgaatgactc attgattttt
     3361 tcacgtaatt gtcgtggtgt caatgttgtc gtatcaatga tcacggaagc ttgggctttc
     3421 aagtcgtcta gtatcgcccg ttctttacgt atcccttcag tgatcaaacc gtccatcgcc
     3481 attggatgtg ctcgtcttgt ttctttgtag cgagccacta gttcttcgtc tgaagcatct
     3541 aaaaataaga tagaagtgtc gatgaagttc gtattttcga tttcgatcag catgttttgg
     3601 atttcttcaa agaaggaacg agaacgcaaa tccactccta aagccatttt tgtgacttta
     3661 cctgattctt tgatcaattc ccagaatttt ggaatcaatc gaggcggcat attatcgata
     3721 caaaaatagc ccatatcttc aaaactttgg atagctactg ttttccctgc accactcata
     3781 cctgttatga tgactaattg caaattatct gtcatattta cgaacccctc tcacattgtg
     3841 cataataaaa ttatatcatc ccgggcgtat cattaccagt tttctttttc ttttacaaaa
     3901 agatttggac caaatatctt ttaggatatt tgccccaaat ccaatttttt tacttttccg
     3961 atgctgtatc caatgcctcg cttctttcag ctaatgcacg ttcgatcgtt cgatccgcta
     4021 aaatagattg agcatcgatc actgggtaat gattatcttc agcaaattca tgcatcaaag
     4081 ataattctgt gcaacctagg atgacttttt cacagtttag gcgctctacc gcttcctcta
     4141 agatttcgta atagagctct tgattcaaat aatccgattc cttgatctca tgatagatca
     4201 ggtaattgat tttttcttga agcgcagtat caggaatcac tgtttcgaaa ccaagatttt
     4261 tgacttcgcg ttcatagatt ccagctttca tacttccttc tgttcctaga atcgctactc
     4321 tccctgtcgt atgctgacgg acaagttcat ttgctgcttc tcttggcata tgcaagatag
     4381 gaatatccgt tgccgcttgt aactcttcaa aaaagtagtg tgcggtgtta caagtcaaca
     4441 caatgaagtt tggtcgcaat aaattttgtt tctcaatatc atccagcaga aacggcattg
     4501 gattttcttc tgatcgatct aaaatataag ctgtacgatc aggaactgtt gcatgattga
     4561 ataagacata attcaaatat tcttgatctt ttgtagcttt cgttcggtgg ttgatcaagc
     4621 gaacaaaact ttctgtcgcc atcgtgccca ttccgcctaa aatactgaaa aaattctcca
     4681 tgaactagcc ttctttcaca tgaaaatact ttttgaatct tggaatatag ttatggaaca
     4741 tatatttcat caacaaccag cgcttgattg atcggtcttt ttcataaaat acagtagtac
     4801 cgtaacgatt ttctttgatc atttgctcag ctagtttttt gtcttcattt tcccgagcat
     4861 attctaaaaa gatttttttg ggcacaccca tccataatct tgctttatct gattgattcg
     4921 ttccataggt cgtttcaaca aagggtttgt tgaacactcg atcttctgtg acaaaacgag
     4981 ccagattcaa gccgttcaat gtcacaaaga aactgctacg accttgtctc aaattgattt
     5041 caaataattt atattctcca tctctaggat catacttcat atcgaaattg gcaaagcctg
     5101 tatattcgat tttttccaaa aatgccttaa tggtttgata gattttttcg ttataatctg
     5161 gcatgatcac aacgtaatta ccaatcgatg ctggtgtagg atcttctaat agtggatgac
     5221 caaggcacat catccgaact tgatgatctt catctacata cgcatttagc acacgcatat
     5281 tactatcatc acccgggata aaatcttgta caatcatttc acttttataa ccggcttcgt
     5341 atatccggcc taaaattaaa tcgaactctt ctctagtctc taaaataaaa gctttttttc
     5401 ttccttcaaa ttggacagaa agatactcca cactgtttgc aggtttcaaa gcaactggga
     5461 attcaaaagg caattcctgt tccaaatgtc catttactaa catttcttct ctgacgatca
     5521 atgttttagg atacggaaga tcatattctt cacaaacttc ataaaagctt actttattga
     5581 tcagcctttc aaataaagaa taatcaatat atggacagat aaaatattct gacagctctt
     5641 gtttatgttg ggagatcaat tctgcatatc catcaccaca agcaatcaaa agatattttt
     5701 tactcttatc agaatatctt tcttttgcca aacgaagcat cgtctcgata aatacagggt
     5761 ctttatcaaa tcctggaata acttctacat tgacaatttt actgtagcgg gtaggggcta
     5821 actgatccga agcatatgct tggcagattt tgccgtatgc ttcattaaaa gaacgtgcca
     5881 taccataaac gttcatgtcg cttcctaata atatagggat aaattcttca ttttcaatac
     5941 tgttcatcat tatctctcac ttactcataa tctcttgttt ttcttttcca actacacaag
     6001 gaatatcata ccataaagaa aaaagcaatc aaaggaaagt cctccgattg ctcaattctt
     6061 ttttgaacgg attgaactta taggaagttt ttatctatac caatcagtgt agaaagaact
     6121 accatgtaat gattctagaa cactgatatt aataaaacaa gcaaaaattc atttttttat
     6181 gtttttttaa ctatctcagc tatctatcct ctttatacca ttattttttt caaatagtga
     6241 cctgtataac tttcttttac gttaatgatt tcttctggtg ttccagtcgc aaggatcgtt
     6301 cctccacctt ctccaccttc tggtcctaaa tcaatgatat aatctgctgt tttgattaca
     6361 tctagattgt gttcaatcac taaaactgtg ttaccagcat ctactaatct ttgtaataca
     6421 tgcaacaagc gggcgatgtc atcgctatga agtcctgtcg ttggttcatc tagtatatag
     6481 aaattctttc cattagagat tttgtgcaat tcactggcaa gtttcatccg ttgtgcctca
     6541 ccaccggaca atgtcgttgc tggttgcccc atcgtcacat aacctaagcc aacatcaaca
     6601 atcgtttgca gtttgcgatg aatctttgga atgtgcttga agaattctac agcatcctct
     6661 actgtcattt ccaaaatatc agcaatgctt tttcctttgt aatgcacttc taatgtttca
     6721 gaattatatc gtttaccatg acatacttca caaggaacat agacatcagg caaaaagtgc
     6781 atttcgatct taataattcc atccccgcga caagcttcac aacgaccgcc ttttacgttg
     6841 aaactaaaac gccctttctt ataaccccgc atttttgcct cgttcgtttg agcaaataac
     6901 ccacggatat catcaaatac acttgtataa gtcgctggat tacttctcgg cgtccggccg
     6961 attgggcttt gatcgatatc gatgatcttt tcgatacttt cgtagccgga aattgtcttg
     7021 aatttacctg gcttagcaga attcttattt aatttttgcg ctaacgattt ctttaagata
     7081 ctattgacca atgtactctt tccagaacca gacacacctg tgactgctac aaattctcct
     7141 aatggaaatt caacactaat atttttcaga ttgttttctg aagcaccagt gatcttgatt
     7201 gctttcccat tacctttacg gcgttctttt ggtacaggga ttgatttttt ccctgaaaga
     7261 tatttccctg tcaaagaatt ttcatcatta gctacttctt caggagtgcc tgcagccacg
     7321 atttgtccgc cttgttctcc ggccccaggt ccgacgtcaa tcaaataatc tgctgcccac
     7381 atcgtatctt catcatgttc gactacaacg agggtgttgc caagatcacg cattttcttt
     7441 aatgagccaa tcagtcgatc gttgtcgcgt tgatgcaatc cgatcgatgg ttcatctaaa
     7501 atatacaaaa ctccagaaag attcgatcca atttgtgtag ccaatctgat tcgctgcgct
     7561 tctcccccag ataatgttcc ggaagcgcga ctcaaagtta gatagtctag tcccacattt
     7621 tccaagaatg aaagtctgtc atttacttct tttaaaatcg gtttggcaat tactttctct
     7681 tgttcagata acgttaagtt tgagaagaaa tctactgctt tttgaataga caactcattt
     7741 gtttgcccga tattcgttcc attgatttga acagatagag cttggggatt caatcgataa
     7801 cctttacatg tctgacaagt taattctgtc atataaagac gcatttgctc acgcgtgaaa
     7861 tcactgttcg tatcatgata acgacgctcg atattcttca ataccccttc gaaagtcgtt
     7921 tctacatcgc gaacgccacc aaaatcattt tcataatgga aatggaaaag atcatctgtc
     7981 ccattcaaga tgatctcctg atgctcttcc ggtaattctt cgaatggtgt atctaaatcg
     8041 ataccgaaac tttcacaagc ctgcgctaac atttgaggat aatactgaga gctgattgga
     8101 ttccatggaa cgatagcacc ttgactaagt gtcttcgttc tatctggaat cactaaatct
     8161 tgatcgactt ctaatttgac acctaaacca tcacagtctg gacaagctcc aaaaggcgca
     8221 ttgaatgaga acagtcttgg ttctagttcg ccaacagtaa atccgcaata aggacaggca
     8281 tagtgttcgc taaacaacat ttcttcttca ccgatcacgt caaccagagc atagccgtct
     8341 gctaatcgca gggccgcctc taaagaatca aataggcgag aacgaatccc ttctttcaca
     8401 atgatccggt cgatgacgat cgcgatatca tgttttttgt ttttctccag ctcaggcact
     8461 tcacttaaat catagatctc accatcgact cgaacgcgga cgtatccctc tttttggatt
     8521 cgttcgaata tttttttatg ttgtcctttt ttctttacca cgatcggtgc caacacttgg
     8581 atctttgtgc gctcaggcaa agccattact tgatctgcca tttgatctac tgattgactg
     8641 gtaatctcga tatgatcgtt gggacaaatc ggatgtccta cacgtgcaaa caacaagcgt
     8701 aaatagtcat tgatttccgt cacagtacct actgtcgacc gaggattttt acttgttgtt
     8761 ttctgatcaa tagaaatcgc cggactcaat ccgtcgatgc tatctacgtc cggcttatcc
     8821 atttgtccta aaaactgacg agcataagca gaaaggctct ctacgtatct ccgctgccct
     8881 tcagcgtaca gcgtatcaaa tgctagggaa ctttttccag aaccggacaa acctgtaacg
     8941 accacgaatt tatctcgtgg aatcgttaca tctatatttt tcaagttatg ggcacgtgca
     9001 ccatgaatcg taattttatc gttagccatg ttgtcctcct actttgaagc ttttaattct
     9061 aaaatcatat ctcgcacatt cgcggctgtt tcaaaatcca aagcttttgc cgcatctttc
     9121 atttcttttt ctaatttcat caataacgtg tctttttctt ccttcgtcat ttcttcgtac
     9181 gagacttgta ccgcttcttt tgtatcatct tccgattctt tcgtaataga aatcaaatct
     9241 cggatttctt taatgatcgt ttttggaacg atcccatgtt cttcattgta cttctgctga
     9301 atcgtacgac gtcttgacgt ttcatccatc gctagacgca tagaatctgt tactttgtct
     9361 gcgtacatga ttactttacc ttcttcattc cgagcagctc gtccaatcgt ttggactaag
     9421 gaacgttcac tacgtaaaaa gccttcttta tccgcatcca agatagcaac aagggaaact
     9481 tcaggtacgt ctaatccttc acgaagcaaa ttgattccaa ctaacacatc aaattcacct
     9541 aatcgaagat ctcgaatgat ttctgttcgt tccagcgttt tgatatcact atgcaaatat
     9601 ttgactttga tccctaattc tttgaaataa tctgtgaggt cttctgccat ttttttcgtc
     9661 aatgtcgtta caaacacccg ctggtctttt tcgactcgtt catttatttc gcctactaga
     9721 tcatcgattt gtcccatgat tggacgaact tcgatcactg ggtcaagcag ccctgtcgga
     9781 cggatgattt gttggataac ggtatctgtt tgttcatgtt catatggtcc tggcgtcgca
     9841 gagacataaa tgatctgatt gacatgtttt tcaaactctt ctaaccgaag tggccgattg
     9901 tctaaagcag atggcaaacg gaaaccgtaa tctactagca tctgttttct cgctcggtca
     9961 ccattataca ttcctctgat ttgaggcatg gttacgtgcg attcgtccgc cacgatcaaa
    10021 aaatcttcag gaaagaaatc caatagcgta taaggcggtt caccttcttt acgtccgtcc
    10081 atatgccgag aataattttc aattcccgac gtgtagccca tttctaacat catttcaata
    10141 tcgtaattgg ttcgctgttc taaacgttga gcttccagaa gtttgttttc atttcgcaag
    10201 acagttaaac gctgttctaa ttcctctttg atattcgcta cggcatgctc catatgttca
    10261 tcattcgtca caaagtgagt cgccgggaag attgctacgt gttccgtttc tcctaaaact
    10321 tcacctgtca gtgcattgac ttctcggata cgttcgacct catctccaaa aaactccaca
    10381 cgtaaggcac gttcatctct tgaagccgga aaaatctcca cgacatcacc gcgcactcga
    10441 aaacgaccgc gctgaaaatc tatatcattt ctttcgaatt ggatactgac taagtcacga
    10501 atcaactgat tgcgatctag ttctgctccc tgtcgaatcg aaactacttg tttttggtac
    10561 tcaaaaggcg agcctaatcc aaaaatacat gaaacggatg caatgacgat cacatcattt
    10621 ctttctaata atgaactagt agccgaatgt cgtaatttgt cgatctcatc atttacactt
    10681 gaatcttttt cgatatacgt atcgcttgaa ggcacataag cttcaggctg ataataatca
    10741 taataagaaa caaaatattc aacagcattg ttagggaaaa attccttgaa ttccccgtac
    10801 agctgaccag ccaatgtttt gttatgggca ataatcagtg ttggtttatt cactttctca
    10861 ataagattgg aaatcgtata tgttttccct gttccagtcg cacctaataa gatttgtgct
    10921 tttttcccgc cgactacgcc atccactaat tggttaatcg cttcaggctg atcaccagcc
    10981 ggttggtatt tcgagacaag ctcaaagtgt cgggaggtat ctctctcaat cattcttcgt
    11041 cctccttttc attcattcct tttcatttta cgaccaataa aaagaaaagg aaagcaaagg
    11101 cattccttac taaaaaatta taccacaccg aacatacttt cgccactttt ttatcttatt
    11161 atacatgtta tgttttataa cataaaatgt tagaaactcg catttttttc attttttcga
    11221 gcttacagtc ttgtctgatt cagcactttt cgcctataat aataaaatat tggaaattta
    11281 ggaggttgat tatgaaaaga aagcatttat ttcactcatt tgttttctta atgacaggac
    11341 ttgtgacact tgcgtttggt catgttgcac aagctgacaa cactacaacg gcagagtcaa
    11401 gcacaagcga gacaatgcaa aaaatagagc ccaaaaaaga cgtatacgta attgccagtg
    11461 actctgcttt tgctccattt gaatttcaaa acgcaaaggg cgagtacgaa ggcatcgatg
    11521 taaaattgat ggaaaacatc gccaaactgc aaggatttaa catccgaatg gactaccgtg
    11581 gtttctctgc tgctttgcaa gctttggaat caggacaagc agacggaatg attgccggaa
    11641 tgactgtaac agatgaacga aagaaaagct atgatttttc tgattcttac tttgatagcg
    11701 ggatccaatt agctgtcaaa aaaggagata cttcgatcaa gtcttatgaa gatttgaaag
    11761 gaaaaacagt cggagcaaaa gttggtaccg aaagtgctga atttttaagc gaccatgaat
    11821 ctgaatatgg ctattctatc aaatattttg atgctgctga tcaattgtac gacgggttaa
    11881 aagttggcgc aatcgatgca atgatggatg attatccggt aatcggttat gcgatcaaac
    11941 aaggacaaga attggaaaca ccgatcaaaa aagaatctgg cggaaaatat gcatttgccg
    12001 ttaaaaaagg acaaaatcct gagcttctta atatgttcaa tgaaggattg aaagaattaa
    12061 aaagaacagg cgaatacgat aagattatta aagaatatgt agcggatggt tcagaaactt
    12121 ccagtgagtc ttccactgct gatgaatcaa catttcttgg acttatccaa aacaactatc
    12181 aagcattgtt gaaaggactt tggagaacaa ttctcctaac tttgatctcc tttgctttgg
    12241 ctatagtaat cggtgtgatt tttggattat tcagtgtagc accaatacgc gctcttcgca
    12301 caattgctag tatttatgtc gatatcatcc gaggaattcc aatgatggtt cttgcgttct
    12361 ttattttctt tgggttgcct ggtatcgttg gatttacgat tcctgacttc cttgctggtg
    12421 ttatcacctt gactcttaac gcaagtgctt atatcgcaga gattgttcga ggcgggatca
    12481 acgccgttcc tgtcggacaa atggaagctt ccagaagttt aggtctttct tataatcgga
    12541 caatgcaaaa aatcattttg ccgcaagcga tacgcatcat gatcccatca ttcgtcaatc
    12601 agttcgttat ctcattaaaa gacacaacga tcatttccgc aatcggtgta gtcgaattgc
    12661 tgcaaactgg taagatcatt gttgcacgta caacacaaag tacgtatgtt tacttgatca
    12721 ttgcgatcat gtacttgatt ttgatcacag cattgacaaa attagcaaaa gttttagaaa
    12781 agaaggtgaa gtaagatggc agaaaaaatc ttagttgagc atttagtcaa aaaatacggc
    12841 gataacacag tattaaacga catcaatgta tccatcaacg aaggagatgt cgtctgtatc
    12901 attggccctt caggttcagg aaaaagtaca ttcttgcgtt gcttgaatcg tttagaagaa
    12961 ccttctgacg gtgaaattat tattgacggc gcccacttga tggataaaag cacagatatc
    13021 aacttggtga gacaacatat cggaatggta ttccaacact ttaacctatt ccctcatctt
    13081 tctattttgg aaaacatcat tttagcaccg atggacctga agaaagaatc tagagaagaa
    13141 gcagagaaaa aagcaatcaa attactgcag acagttggtc tagaagataa aaaagatatg
    13201 tatcctgaaa tgctttctgg tggacaaaaa cagcgtgtgg ctattgctcg tgctctagcg
    13261 atgaaccctg acatcatgct ttttgatgag cctacttctg cactcgatcc tgaaatggtc
    13321 ggtgatgtct tgaacgtcat gaaaaagtta gcagaacaag gtatgacgat ggttatcgtt
    13381 acgcacgaaa tggggttcgc taaagaagtg gccaatcgcg tcatgttcat tgacgacgga
    13441 aactttttag aagatggaac gcctcaacaa atctttgaaa atccgcaaaa tgaacggaca
    13501 aaagatttct tgaataaagt attaaatatt taataaaaac caaaaatgca cggtcgccat
    13561 gaagggaccg tgtattttta tcttcttatt ttctcaccta atcgtatcag ccgcatcgct
    13621 tgttttcctg cacaaacaag caatcgctct acctgttcca tcgcataaga aagtaccatc
    13681 ggttcattga tgatatcaag tacatagtcg ataccaccct cacttaattg agcttgattt
    13741 ttcttctcca tattatgtag ttgtgggaca actttctgac aatcagcaat tgtgtcaaga
    13801 acggatcaat cctttattgg agttgctcga aagggtcttc tcttattacg gacaggcttt
    13861 gctcactgta catagacaac aaatcattat cctagtcaat cggatttcca gagcctcatt
    13921 gctatcattg cttgataaaa tccagacaaa gtttaataaa atgtatcagt tacagctaaa
    13981 ctttggaatt gggtcgcttt gttatacaga gcaagagaca ccgcaatctt tccttcatgc
    14041 caaacaagtt tgcgaatgga tagctttcca tcaatctgtg aacgagatcc gcttttttga
    14101 agatttagac ctcgggatcg ttttaccagc aatcccgtca gatcaacgaa cattatatgt
    14161 aaaacgtatt ttgaaaagcc tgacagaaga agaagtacat ttgttcaaga agactttggc
    14221 atgcttcagt aaaaataacg gaagcatcaa aaattgctca gaagaattat ttattcataa
    14281 aaacaccttg caatatcgat tgaacaagtt ccattctctc actggttata cccctagaaa
    14341 ctatgacgat tatcatattc taaagctggc ttttttgttg gtacaaacat gacaactaca
    14401 aaaaaaccag gcgaacatat gattgttcgt ctggttttta attattaatg acttagcgtt
    14461 tttactagaa aaactacttc cgctttttcc cgccaaaagc atctttaaac ttatcgaaga
    14521 aaccgtcttc ttgctgttct tcaactttga tgccgccagc ttcagcgaat gcacgtaatg
    14581 cttctctttg ttgttcattt agatttttcg gtgtgatcag ttttactttg acatgttggt
    14641 cgcctgttgc accaccacgt aacttcggag cgccttttcc acgtaaacgg aagttcgtac
    14701 ctgtctgtgt tccagcaggg atcttcaatt tcacatctcc atggacagtt ggtacttgga
    14761 cttcatcccc caaagctgct tgtacaaaac ttaaaggcaa ttcatagtaa atttcagaac
    14821 cttcacgatc aaagatatca ctttcttcta cacggaagac cacatacaaa tcaccaaatg
    14881 gtccgccgtt ttctccagct tctccttgac cagctaaacg catttgttgt ccttcttcta
    14941 caccagctgg tacattcact ttaactgaat gtgctttctt ctcatgtcct gagccatgac
    15001 aggttggaca aggttctttg atttctttcc ctgtcccatg acaaacatca caagtttgac
    15061 ggctcatcac tcgtccaagt ggtgtttggc gttctacatt aatcgtacct gcaccatgac
    15121 atttatgaca agtagttggt tgtgtgcctg gttttgcacc gttcccacca caagtatgac
    15181 agatttcttc ccgattatac ttgatttctt tttctacacc aaaaatcgct tcttcaaaag
    15241 acaagtcgac cgtatattgc aaatcagcac cttgacgagg cgcatttgga tcgactgacc
    15301 gaccgcctcc tccaaagaaa gagtcgaaga tatcttcaaa accaccgaag ccgccaccag
    15361 agaagccgcc gaatccacca aatccgccac cagctccacc agcaccgtaa tttggatctg
    15421 tgcctgcatg cccgtattgg tcatatgcag cgcgtttttg cgggtcactt aaaatctcat
    15481 atgcttctga tacttctttg aatttttctt ccgcatcagg ttctttattg atgtcaggat
    15541 ggtatttttt cgaaagctta cgataagctt tctttatttc atcttctgat gctccttttg
    15601 agagccctaa gacttcataa taatcacgtt ttgttgccat aggctttcct cctattggtt
    15661 ctcatcattt tcacgtcgct tgttatcata tcatattttg tttgaaacaa aaaccgatac
    15721 ttcaaactat ttttcaaaag aaaaagccaa agtagcaact ttggccttct ctcgaaattc
    15781 agtgaactga ttatttatcg tcgccatcga cttcttcaaa atcagcatca acgacatcat
    15841 ctgctccgcc ttgagctgct tctgggtttt cttgagcttg ttgttgtgcc gcttgttcgt
    15901 atagtttaac agtcaagttt tgaacgattt cgttcaatgc atcgcgttta gttttcattt
    15961 cttcgatgtt gtttgcttca acagcggctt tcaattcatc gcgagctgtt tctgcttttt
    16021 tcacttcttc ttcgtccact ttgccttcta attctttcaa tgttttgtcc acagagaata
    16081 gcaatgcatc tacatcatta cgtaggtcaa cttcctcttt acgagcttta tctgcttctg
    16141 catttgcttc tgcatctttt accatacgtt cgatttcttc gtctgttaga cctgaagaag
    16201 atttgatcgt gattttttgt tctttttgtg tacctagatc tttcgcagat acatttacaa
    16261 tcccgttttt atcaatatcg aatgttactt cgatttgtgg aataccacga ggagctgctg
    16321 gaatatctgt caattggaat cttcctagag ttttgttgtc agcagccatt gggcgttcac
    16381 cttgtaatac atggatgtct actgctggtt gattatctgc cgcagttgag aatacttgtg
    16441 atttacttgt tggaatcgtt gtattgcggt caatcaattt agtgaacact ccacccattg
    16501 tttcgatacc taatgacaat ggtgtaacgt caagtaaaac aacatctttc acatcaccag
    16561 tgatcacgcc accttggata gcagcaccca tagcaactac ttcatcaggg ttaactgatt
    16621 tgtttggttc ttttcctgtt tctttacgta ctgcttctac aactgcaggg atacgagtag
    16681 atccaccgac taaaataact tcgtcgattt ctgattgtga taaacctgca tcttttagcg
    16741 cttgacgtac tggtactttt gtacgttcta ctaagtcagc tgttaattca tcaaattttg
    16801 cacgtgtcaa tgtcatttcc aaatgcaatg gtccagattc tcctgctgtg ataaatggca
    16861 agctgatttg tgtgcttgat acgccagata aatctttttt cgctttttca gcagcgtctt
    16921 tcaagcgttg taaagccatt ttgtcttgag acaagtcaat gctgttttct tttttgaatt
    16981 ctgctaccat gtagtcgatg attttgttat caaagtcgtc cccacctaga tggttgtcgc
    17041 cagctgttga caatacatca aagacaccgt ctcctagttc aaggatagat acgtcaaaag
    17101 taccgccacc aaggtcgaat actaagattt tttcgtcgcg atctgtttta tctaaaccat
    17161 aggcaagagc agctgcagtt ggttcattga cgatacgttc tacttctaga ccagcgatct
    17221 taccagcatc ttttgttgct tgacgttgtg catcattaaa gtaagctggc acagtgataa
    17281 ctgctttttc gactttttca cctaaatagt cttctgcgaa tcctttgata tattgaagga
    17341 tcattgctga gatttcttgt ggtgtatatg atttaccttc tacatctact ttgtaacctg
    17401 cttcaccgat atggcgtttg attgatgaaa tcgtatttgg gtttgtcaca gcttgacgtt
    17461 ttgcaacttc ccctacttgg atttctccat ttttgaatga tacaacagaa ggtgttgtac
    17521 ggtttccttc tgggttagcg atgatttttg cttcgtttcc ttccaatact gctactgcag
    17581 agttagtcgt acctaagtca ataccgataa ttttactcat aattaacgtc tccttcttta
    17641 attgaaagtg taagttttat atccgcacaa ttattgtgca acaatgacca tggttggtct
    17701 taacacgcga tcatgtaatt tgtagccttc ttgtagtact tctacaatcg tatcggctgg
    17761 tgtatcttca gtcgctggaa cagtttgtac tgcttgatga agattaggat caaaggcttc
    17821 tcctttagca gggatttttt caattccttc ttcttccagt gcgttacgca aactttccaa
    17881 gaccatttcc acgccttttt tcaaacctgc cccttgatca tctgacactt ctgtggctaa
    17941 tgcacgttcc aggttgtcaa ttgatggaag taattttttg gctaaatctt gtgaacggta
    18001 tttttgtaat tgctcacgct cattttttcc tcgatttgcc atatttgcga tttcagcacg
    18061 agcccgcaag tatttatctt ccatttcttc taactcagct tttaatttcg ctgtttcgaa
    18121 ttcttccgct tcagcttctg aaatacccga atcttctgca gcttcgacat cgatttccgg
    18181 ttctggctga gcatctgtca tttctttatc tagttcttct tgattttctt tcacgataca
    18241 gtcttccttt cattattcag tcagctgttt gattgctctt actaagaaag agagaacgat
    18301 cagctgcaaa tagcttcgtt attcacatta cgagtttgac aagtcaagcg aacggtagta
    18361 atcagcaagt ttagctgcta gttctcttcg ataaacatcg actagaccga acatccgaga
    18421 ataaggcata ctagtaggcc ctaacaaggc tattgtacct cttccatgac catctatctc
    18481 gtaacttgct tgaatcatac tcatattctg caataaatca ttgcctaatt cagcaccaat
    18541 cttgatatga atattgctat ccatcggagt gatcatttgt gtaagtccgt ctgagtcttt
    18601 cataaaagaa aacatcgatt tgaactgatc aagatcttga cggggctcat aatccaagag
    18661 attcatttgt ccactgacat aaaccttatc ttcaaaaaca tgaccaagaa cggtgtcaaa
    18721 taactgagac atcccttctg tcgtttggaa atatttatga agaatcatcg gaatctccgt
    18781 acgaagttta tggtacaccg tcatcagagg atctccgact agacggtcgt ttacaatctg
    18841 taccattttt tctagttcct gacttcccat atattcggga agagtgaaca cttggctttc
    18901 tacattcccc atatctgtta cgacgatagc aataatctgt cgattattca agggtacgat
    18961 acgaaagcca gtcagtcggc ggtcttttat ttcaggtccc aatgaaaaag tcgtatagct
    19021 tgtcagctca gataatatct cagcagattg cttaataatc tcattgattt catgaaattc
    19081 tctgcctaga gactgacgaa tcagcagcaa atcatcttct gctacgcgag caggttttaa
    19141 caaatgatcc acgtaataac gatagcctaa agcagatgga atccgcccag aagaagagtg
    19201 agtcttcaac agcaatcctt catcttctaa tgctttcata tcattgcgaa tcgttgctga
    19261 gctggccttg actccctcag acatcaatgt tttggaacca acgggcacgc cactactcgt
    19321 atagtgttgg atgatcagac gtaaaatgtc gttttgtcgc tttgttaaca tcttcctcac
    19381 ctctcattag cactcatata ttctaagtgc taatccactt ataaatatag caagaagccc
    19441 ttctcctgtc aagagaaaga ctccattttt tagcactcta aacacaagag tgctaattct
    19501 aaataattga tgtaaatcca gttatatcaa cgtttcaata gtcctttatt gtatttttga
    19561 acttttttta ttta
//
LOCUS       DACEMO010000030        19526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEMO010000030 DACEMO010000000
VERSION     DACEMO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.030"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(62..1246)
                     /locus_tag="IW728_000847"
     CDS             complement(62..1246)
                     /locus_tag="IW728_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1024224.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1271..2278)
                     /locus_tag="IW728_000848"
     CDS             complement(1271..2278)
                     /locus_tag="IW728_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1024225.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2450..2809)
                     /locus_tag="IW728_000849"
     CDS             complement(2450..2809)
                     /locus_tag="IW728_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024226.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(2781..3152)
                     /locus_tag="IW728_000850"
     CDS             complement(2781..3152)
                     /locus_tag="IW728_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1024227.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3169..3471)
                     /locus_tag="IW728_000851"
     CDS             complement(3169..3471)
                     /locus_tag="IW728_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024228.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(3488..3958)
                     /locus_tag="IW728_000852"
     CDS             complement(3488..3958)
                     /locus_tag="IW728_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1024229.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(3933..4211)
                     /locus_tag="IW728_000853"
     CDS             complement(3933..4211)
                     /locus_tag="IW728_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1024230.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4208..5257)
                     /locus_tag="IW728_000854"
     CDS             complement(4208..5257)
                     /locus_tag="IW728_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1024231.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5214..6143)
                     /gene="cpaF"
                     /locus_tag="IW728_000855"
     CDS             complement(5214..6143)
                     /gene="cpaF"
                     /locus_tag="IW728_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1024232.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6275..8029
                     /gene="ade"
                     /locus_tag="IW728_000856"
     CDS             6275..8029
                     /gene="ade"
                     /locus_tag="IW728_000856"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1024233.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8127..8789)
                     /gene="trmB"
                     /locus_tag="IW728_000857"
     CDS             complement(8127..8789)
                     /gene="trmB"
                     /locus_tag="IW728_000857"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1024234.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(8859..9638)
                     /locus_tag="IW728_000858"
     CDS             complement(8859..9638)
                     /locus_tag="IW728_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1024235.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(9845..11056)
                     /locus_tag="IW728_000859"
     CDS             complement(9845..11056)
                     /locus_tag="IW728_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024236.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11049..11786)
                     /locus_tag="IW728_000860"
     CDS             complement(11049..11786)
                     /locus_tag="IW728_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024237.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            11924..12358
                     /locus_tag="IW728_000861"
     CDS             11924..12358
                     /locus_tag="IW728_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1024238.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12351..12695
                     /locus_tag="IW728_000862"
     CDS             12351..12695
                     /locus_tag="IW728_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1024239.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            12875..13885
                     /locus_tag="IW728_000863"
     CDS             12875..13885
                     /locus_tag="IW728_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1024240.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(13954..14898)
                     /locus_tag="IW728_000864"
     CDS             complement(13954..14898)
                     /locus_tag="IW728_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1024241.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(14882..17602)
                     /locus_tag="IW728_000865"
     CDS             complement(14882..17602)
                     /locus_tag="IW728_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1024242.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(17599..18819)
                     /locus_tag="IW728_000866"
     CDS             complement(17599..18819)
                     /locus_tag="IW728_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1024243.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(19100..>19526)
                     /locus_tag="IW728_000867"
     CDS             complement(19100..>19526)
                     /locus_tag="IW728_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAQ1024244.1"
                     /translation="EKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKANIFNHLK
                     KMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESA
                     DISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         5936 a         3954 c         3247 g         6389 t
ORIGIN      
        1 aacacatgca agaattccct cttgcatgcg tttcatcttt tatcctaacg aatgctttgg
       61 cttagccgcg cagacgctcc acatcacggg caatcattag ttcctcatct gttgggatca
      121 ctaatactct tacttttgat tcttttgtag agatatctac ttcttcacca cgcaattcat
      181 tcaacgctgg atcgatttca caaccaaacc aagacatacc tttgatgatt tcactacgta
      241 catgtccgtc attctcgccg ataccagcag taaagacaat tgcatcaaca ccattcatcg
      301 ttgtcacata accgccgatg tattttcgga tgcggtccac gaaaatgtca taagctaaac
      361 gaatatgttc tttttcatag ttcttttcaa gatcacgcat atcactagac aatcctgtta
      421 ggcctagcag accagatttc ttgttcaaaa cattgatcat ttcatcaata tcaatctcta
      481 atttttccat taaatatggg agaacagctg gatcgatatc acctgaacgt gtacccattg
      541 tcaccccagc taatggtgtg aatcccatag aagtatccat ggatttacct ccatcgactg
      601 ctgtgatcga tacaccattt cctaagtggc aagtgatgat ttttagttct tcgatcggtt
      661 tgttcaataa ttttgccgca cgttccgaca cataacgatg acttgtgcca tggaagccat
      721 attttcttac tttgaaatct tcataataag acaccggtaa gctatataaa taattttgct
      781 ttggcattgt tgagtggaaa gatgtatcga atacagccac actcaaaatg tcaggtaaaa
      841 tctttttaaa cgctttgatc cccattaagt ttgcaggatt atgcaaagga gcaaattctg
      901 ctaattcagc aatgcggtcc attactttct catcgatcac tactgaatcc ccataagttt
      961 ctcctccgtg aacaacacgg tgccctactc cggtgatttc atcataagag cttaaaattt
     1021 gcagttcaat caactgatct aacagcattt ttactgctac ttcgtggttt tcgatatcca
     1081 tgatttgttc gaatttttga ttatcgccgt acttgatggt aaaaatagaa tcttgtaaac
     1141 cgatacgttc gacgatccct ttcgcaacta cggtttcttc tggcatttga tataattgcc
     1201 atttcaaact tgaactccca gcattgattg caattgtttt agacatatga ctctccttct
     1261 cgttgaatga ttacagagca gatgatttcc agtttttaaa ctcgttgaaa aattccgtca
     1321 ccttctctgg ttcttttaat gaagcaagtt tcacaagcaa gacttctttt acttgttgtg
     1381 cttgtggtcc ttttttctgt aagatcaaaa tacttttttg cgattgttta ttacggaaca
     1441 attcatctgg caattgaatc attccttgaa gataaccttc ttctttaaac cattttttga
     1501 tttcttcact ttgatccgtt tctaaaaatc cactcggcat caaaaacaaa ccaaaaccat
     1561 ccggcttcac atatttcatt gattgctcta gcaataaatg atgtgcataa ctatggcctt
     1621 cttcggtact tgttaaaaac tcttttgctt tttcgtcatt cggataataa ccgattggca
     1681 aatcgctgat tgcaaaatcg acaggatcta ttaataattc ctgcaacccg tcttgatgaa
     1741 agtattgaac atttgcttgt gtcaaatcac ttgtagaagc tgcgacagcc aacaatgtat
     1801 catcaatatc cacaccaatt ccttctgttt ggtaccccgc attcgatagg tttaaaagaa
     1861 ctgttaataa caagttgccc attcctgccg caatatccag aatcttaacc ggtgcttttt
     1921 tatttgtgta tagttgttca ataagaaaaa caaataaaaa accgatacta tcaggagtga
     1981 gttgatggtt cgcctgcaaa tgttctgtct ggctgccctt caataaaagt aattgagaca
     2041 gtctgcgcca ttcttctggc tcgaaagaaa gtttcttcaa ctcttcataa agagctgtga
     2101 tccgttgcgt tgtctctttt gtcggcactc catccaccac acgtacttga taatcatcta
     2161 ttaaattttc tgcattttcg acataggctt ctaaaaagga agttcccaat gcattctgta
     2221 aaagctgaat agcttccagg ttttgattaa acccttgttc cattttttct ggtaacatgg
     2281 tgcacctcat ttctagatat attataacaa ttttttcacc tgtttaattc tatcgaacaa
     2341 ttgacaaaat ttcaatcgtt ttcactgttt tttatttgtg aaacggcaaa cattttttta
     2401 ttaaaaacag tgttataaca gaaaagaggg cgttttcacg aacattccat cattctgtac
     2461 tagtatctgt atcatttttt acttcttctg tgaaccgaaa gataaacggt gagacttcca
     2521 cctctaagaa tagggactgt tgtacccact gatatgttac agatccagtt gtgtagtaaa
     2581 aaactccttc cgaactttcg gcaagcgtag actgttccga taaagccatc tgcttcatta
     2641 tttttgctat ataataattt ttgattcgta tattaagatc cattgacaaa cggtagcttt
     2701 ctaatgctgc catcaataaa aacgtacata aaagaaataa aagcaagaca gaaaataaaa
     2761 tagagccctt ataacatttt ctactgatcg ttttttgtcc atatggcata tccatcttct
     2821 ccattttcaa agtgtacata aaaagaaatt gtgtgttctt tttccaataa ttgaagctca
     2881 gtgatacttg ttaacatagg ttgatgtccg ccggaagttc ttcttttacg tatcattgcc
     2941 tgatatcttt cgatgatata ctgtttatcc tcgatcgtgt agtgcagttg ctgatttttc
     3001 acagagacat tgtgactgct cttgagttct tcttctaatt gtgctaaaaa tattagccac
     3061 tctttttgat ctttacgctg caaaaattca cttacttgga tggtttgctg aatcaaaaga
     3121 ttgaatgtga gcaatagact gctcaatatc atcagtccaa ttagacattc aatcagcgta
     3181 aatgctggaa ttacttgttt tttcaatact gatcgcttct ttctccatat tgacaaacac
     3241 tttttcgatt actccattat tatcgacaat ttggatatcg tatgggatca tttcttccct
     3301 tcgctctggt cttttgtgat acatccgctg cgtcttgata tcttcataaa tcattcgaat
     3361 aaatagcagt tcctccccac ttttcttttc tgcctgaagc aaaaacagct ggccaaagct
     3421 cactaagtag acacagacag aagcgatcat aagcgctgta agggactcca acaaaacaaa
     3481 tcctgtttta ttcaattctt tttgtatagc gaccactccc tatctggaat tgatacgtaa
     3541 tcgtccgctc tttttcttcc caataaaatt gataagcttt tagtgaactt tcattcccgg
     3601 ttctagcagc aaaagtaata gaagcatggc gtttcaaggt aatctcatca ggaatatcta
     3661 atacttccca atttatttta tctggattcg gaacatgaaa aatgatttga ttctcttcac
     3721 tattccaata aaagcctgtt tggatctgat tcacaatagc gattttttgc gtagaataga
     3781 ttcgtttttc aaattggccg aaaaattgat agactgccaa ttcattcttc catgcagaaa
     3841 atgcgagaac tggaaaagaa aggatcgttg taagtaataa aatcagtaag agtgattcaa
     3901 acaacgtata tcctgatgct aaaaccttac cttcaaggcc gctgatagat ttctgcatgt
     3961 tctcgtttga tatagccttc attcaacaat tcctccacag taggaactct tccattattt
     4021 tgaagcgcat agagctcttt ttgtgtgtct accaactgga tgatcgcttc acgactttct
     4081 tggtctacat gattcctata acgcgacaaa ttagggacga acaacaacac taatacactg
     4141 atgatcagta agacgattag catttcgatc aatgtaaaac cgctataata agatattttt
     4201 ctctttttca tggcaaaacc tcctctatgc ttccgtaaac aggtaataaa acagcggcat
     4261 aaactaataa aatcaaagcc gctacgccca aaaaaatgat tggctgaata aaatgcatcc
     4321 agtgatgaag acgtttgaaa agctgttttc ttgtccattc gctgtaaaaa atcagttctt
     4381 tacctaaact tccctttgcc tctccctgta aaacaatttt actgaattct tctgatagaa
     4441 aacgataatt ctggaactgc tcagccaatg acatacctgc ctccaatcct tgaatcaact
     4501 tagaggcaag atgctgaatc agactttctt gtttcgtttc ctttaagcag actatgattt
     4561 gccttaattc gaaaccttca tgaaaaagct ttccaatctc tagtgacaaa taagccgatt
     4621 gatacaagga atatatcggt ccaatcaata attgtctgct tatccattca ctctgatcaa
     4681 caggagacaa cttcatcaga cgatacctta ctagtaaagc caatagagta accgctagga
     4741 tgaatagtag aacataccaa tgaaagactt gtatcagctg aataccccaa tgctcttgca
     4801 tgaccattcc tgaaccaata agctgaggca aaacgaacaa ccgcatacta aacaagatac
     4861 ctagcaaaaa ggtgagcaat agcatcggat atgcaagcag ctttttcaaa tcattgcgaa
     4921 atccttcaac aagccgaaac tgtttggcaa tttccaacaa agttgtcggc agattcccat
     4981 gacgttcagc taactcgatc tgtaccagtt gctctgctcc atatcctatt tgcgaaagta
     5041 cctcactaaa cgcttgccct tcctttagct gatgttggat atttgccaaa ataacaggtg
     5101 gaaacgaacg gatcgtgagt aataaataaa gggattcttg cagactaaaa ccagataaaa
     5161 gcagttctcc catggtttgt gcaaactggt tttgctggct tcttgttaat cctttagaat
     5221 agttctgact gccaaatatt ttcctgagct tcttcataac tttcctccca tgattttttt
     5281 actgattctt cctccataaa ttgataactc cataaaacag aaggatggtg attggcatct
     5341 tccaaaagtt cttgataaat gacaccagct aaacattcag atagctcttc ttttttcttt
     5401 gtcagttcat ggattctgac caatgtacct tcaagattgc gtgcatgtac ggttgcaaaa
     5461 acccggtgcc ctgttagtgc tgctcttacc gcagcatgtg cagttgattc atctcttatc
     5521 tcgccaatga ttagcaagtc tggacgatgc cgtaatgcga gtctcaaaag tgtatcataa
     5581 gtctgtgcta ttttcggatt gatctgtagc tgcagaaaaa acggttcttc tatctctaca
     5641 ggatcttcga tagtaatcac ttgcaatttt tcttccaaag ccaaccgata cattagagaa
     5701 gtcttgcctg atccgactgg tccgctgaaa aggtataatc cacgtctgac ggttttctgt
     5761 aaaatagttg caaaatctcg agaactaaag caacgatggg gtgtttcaaa agtaccgtat
     5821 aatagccgaa tcactaaact ctctcgttga aggtaatctc ctacagaaga caaccgcaga
     5881 cgaacttcct gtttttctaa agaataagta aatgcaccta actgagcttt tcttttttct
     5941 ccgatatcca tctgtcccaa atacttaaat cttgagatca gttgctgtcc atctgccgaa
     6001 gagactttct tccagaatgt tcgttttttt ccatagcgaa aatagatgta aaatacttct
     6061 gaagctggca atatatatac gtcttgggca tgtttggcaa aactttcact tatcatttga
     6121 tccgataatc gttttatttc cattattctc ccctccacaa aaaacatacg tctttttgat
     6181 ctttttgttt caaataaaat gaatttacaa aaaaatattg gcaaaaaccg aatttatgtt
     6241 atacttataa aaatttatct tgggaggcac tccattggaa acaacattaa ggaaatatat
     6301 cgaagtagca agtaaaaaaa agcctgctga cctagtgatc aagcatgcta agatcgtcaa
     6361 cgtctttacg aaagaaatca tggaaaaaga tgtagcaatc tgcgaaggaa tgatcgtagg
     6421 aatcggtgaa tacgaaggaa aaagcgtaca taatgctaat gggcaatatc tagtgccagg
     6481 attcatcgat ggccatgtgc atatcgaaag ctcactttta tcaccaagcg aattttcaaa
     6541 agtatccttg cttcatggag ttactactgt agtgacagat ccccatgaaa taggaaacat
     6601 tgctgggagt acaggtcttg agtttatgat agaagatgca agacaaacac tcatgaatat
     6661 cttttttatg ctaccttctt gtgtacctgt aacccctttt gagaccaatg gtgcaaattt
     6721 aaatgcaaaa aaattagccc cttttttaca aaaacccgaa gttttaggct tagctgaagt
     6781 catgaactat caagcagtag caaataatga aacagacatc ctagaaaaga tccaattgat
     6841 gcaccagcat aagaaaaaaa ttgacgggca tgcagctggt atcggcatgg aagagttaaa
     6901 tgtttatcct gcagcaggta ttcggacgga tcatgaagct acaactgcaa aagaagctaa
     6961 agaacgtttg gatcttggta tgtatttaat ggttcgagaa gggaccgttg caaaagatct
     7021 ggcatcgcta ctaccggcga tcacacctga aaattcaaga agatgcttct ttgtcaccga
     7081 tgataaactg atcaatgatt tagtaaaaga aggatcaatc gatcatatcg tccgtcaagc
     7141 tattgcactt ggaatcgatc cattacaagc cattcagatg gcaacattga atgcagcaga
     7201 atgttttgat ttgaaaacac taggtgcgat tgcccctggg tatcaagctg atttcttctt
     7261 aacagacgat cttaccaagc tgccaattca tgaagttttc tataagggaa cattagttgt
     7321 tcaatctggg caactccaag accatttatt tgctgatcat tcaaattcat atacgtgttg
     7381 tctgccaaaa ctaaacgctc aaccattaaa tccacattcg ttagagctgc ttctttcttc
     7441 ttcacaagcc catgtgatcg aaattatccc gaatagtcta gtgactaaag atttggtaga
     7501 aaaagtggac cgacaaggcg aacgattcat tccttcaatc gaaaaagacc aattaaagat
     7561 tgcggtgatc gagcgtcacc atcaaacggg aaacattgga ttaggcatag tgaaaggatt
     7621 tcagctgaaa caaggagcga tagcgacaac gattgcccat gactcgcata atctagttgt
     7681 cgttgggaca aacgacgagg atatgctgta tgctgctaat cttttgattc aaaaaggtgg
     7741 cgggatgacc gttgtcaaca aacagcagga aatagcttgt gtttctcttc ctataggcgg
     7801 aatcatgaca caggaacctt tttcgatcgt ctatgaacag ttgaatgaat taataactaa
     7861 agcacatcaa ctaggagcag ctaaaacctt tgatcctttt ttgacacttt ctttcttaac
     7921 attatctgtc atacccgaat tgaagatcac cgacaaagga ttattttcat tttctcgctt
     7981 ccaattgatt ccaccttgtg gaaaaaccga tgaaataaat acattttaaa tcaaataaat
     8041 cccagaaaat acattacatc tgtaatgttc attttctggg atttatttat ggatattaat
     8101 gtttttatga aagcagaaag ttagtttcac agacttttct ttttggtctt atcttgaaat
     8161 tgagcttcta ctcgataaat acgttgacct tttgaagaaa atttttcttc atactccgtc
     8221 ataatattgc cttcaaattg actttgatgt aaatctaacc atacctgttt gaggatcatg
     8281 ccatattgag aaaaacttgc cagagaatac tcaaacaatc cttgattatc tgttttgaaa
     8341 tggatttctc catttggacg aagaatctgt tcatctacag caagaaaagt cttatatgtc
     8401 aatcgccgtt tttcatgacg tttttttggc catggatcgg aaaaattcag atagatttga
     8461 tctacttcgc tgtcagcgaa atactctgtc aacgctgatc catccacatg tagaagtttc
     8521 aggttaggaa gcggttgttc gatcaacttg tctagtgcaa tcgaaacaac actgacctgc
     8581 atttctactc caatataatt gatttcagga tgggcttttg ccattccagt gatgaactgg
     8641 cctttcccca taccaatctc aatgtggatt ggatgatcgt tttcaaacaa ttcattccat
     8701 tttcccttcc acaatgttgg atcactaatt acaaaattcg ggtgagccgc taacatttcc
     8761 gctgctcccg gacgatttcg gacacgcatt atttatctcc attcctttcc tctaattaaa
     8821 aaggtctggc caaatctccg cagagtttgg gccaaacctc agccactgaa aattcgtttt
     8881 aattgtaaaa tttcttcgtt catccgtcga tgatcttcac gtttgcaata tctggtgatt
     8941 tcttggagca aattcatggc cgcataccaa tgaaattttt caagcatttc gtcagtcggg
     9001 cgtatgccat aactgatcag ccactgattc cagctagaca gtggcacata atttcctaaa
     9061 atcgttgcaa tatccaccgc agggtctgca aacatgattg aatcccaatc gactagatat
     9121 aaataatttt tacatactaa ccaattacga tggtttacat ctccgtgtac tactgataag
     9181 ttttccttat ctattgacgg gatctgctca cgaagaaaac gatggacaat ttgcagaaat
     9241 tgatttttct ggagttcttc cggtaattct ttttcatatt tttgcagcat cttctccggc
     9301 gtcatgacac gaccaccgat acgggtcagc atggttttaa gagaatttga atgatgtaaa
     9361 tggtacaaaa catcaatcac gtcgtttctt tgaccaatct cattaggagt cagcaagcga
     9421 ccttccagcc actcttgtgc tgtcaaagta tcgccattcc ccgttcgttt tgtccaaaca
     9481 agttttggtg taattccttc ccttgaaaga gcagccaaca tcggagtcgt gtttcgttta
     9541 ataaagactt tgtcctcatc acgcatgcct atgtatgttt ttcctgtgtc gcctttgatg
     9601 ggacgtaacc gccactcttg gtctaactga aactccatca tgcattctcc cgtcctctta
     9661 ctctttgttt tgcagtactt attattctag tcgcctgaac actacacgtc aagcacatat
     9721 acgttctttt tcatttaatt gccattcaaa aaaccagctt ttctcaaaaa aaatagaacg
     9781 aacagcattc ttactgtcaa cgctctattt ttcattctca tttttaccct acacagcttt
     9841 gttttcagtc tgacatcttc ttcaaacgag ttggtacata tagtttaaca aagagcatag
     9901 taacaatcaa ataagccgct agcggaaccc atcgctgctg cccatctaat acgactgcag
     9961 caatcaaacc aaaaataacc gacgcgataa ttaataccca tgtgagtaga tactgaatcg
    10021 cctgcttctt ctgattctta tggattggat aaagctgaac gagaaccata tagcggaact
    10081 ggttcgctaa aggtaacagt tggaaaccga tcaaatataa aaataagaaa ccgataatca
    10141 gactaaaata aaagtcgact actccggcaa tcaataaccc acctataatt actaatcgga
    10201 aataaagacc actaaaatca gaacctcttg ccaatcttct tgcaaacaag taaagataag
    10261 tgttttttga ttctctttta atatgagtca atataggatc caaatagcgt cttcttttga
    10321 tctttgccgt aatttcagga acatctgtga ataaattgat aaattgatag atctgatgca
    10381 acctatgttc ttctacatgc accattttct cccaatccaa tcctttttct tgttgcttcc
    10441 acaaaaacca gtaatataga cttgcctgta ctaatgcacc tgctattcct gcccatatta
    10501 gccaataaaa tgatagaaac aaaaagatca gagaagaaaa aaaccagcct aatttccaat
    10561 aacgaataac ttgttgatct agtcgaaaca attctgctct cttgatttgc agatgactga
    10621 atttcaaact ccataatatg atcagataga cgaaaaacat cgaaaaggat tgctccgtac
    10681 taaccaccac taatggcatg gccaatccgc atgccaatat caaaaagatc aacggaaaaa
    10741 tgcaagaata gcgaaaagct cgttccaaat aggtttgcat ttctttttct ttaggaagta
    10801 aaaaaacaat atctgctgcc tctgcaaaag tagccatctt acccacgaat aaacagagca
    10861 tccaaaatac agagattatg agccctccaa ttgcaaacgg tggctgcagg gttttcaacc
    10921 aattagaata atataatccc acaccgccaa tcaagaaaag acaaactaaa acaaaatgat
    10981 cgttcaatac ataacgcata tatttcatca ttttcttttg atgtcttgct aaacgttttt
    11041 gaaaaaaact actcatgtgc ctgctccttt gttaaagcaa tatagatttc gtccaaagaa
    11101 gaatccggca gctgaaattc ttttctcaac atctcaagag aaccgtctgc tcgtatctct
    11161 ccgttatgta agaccacaaa acgatcacaa tatttttctg cagtagctaa aatatgcgta
    11221 gacatcaaaa taccggctcc ttcttttttc atttctacca tcaattcaag caatgcattg
    11281 atagctagtg gatccaagcc taaaaatggt tcgtcgatga tatataaaga aggctcgatc
    11341 aaaaaggcgc ataagatcat cactttctgt ttcattccct ttgaaaaatt agctgggaac
    11401 cagtcaagac gattagataa ccgaaaagtc tccaacaact tttctgctct tttcattgct
    11461 atatccatag gtatatcgta ggccatcgca gtgacttcga tatgttctct caatgtcaat
    11521 tcttcatata aagaaggagt ctctggaata tacccaattt gtttgcgata ttcttccgta
    11581 tttttcttta aggatagatg attgatttct atcgttcctt ttgaagcttc cagcagaccg
    11641 ataatgtgct tgatggtcgt acttttccct gctccgttca gtccaatcaa ccctactatc
    11701 tctccaggct gtacatcgaa actgatatct tttaacacag ggagatgtcc gtacccccct
    11761 gtgacatgat cgataattaa actcattctt tttcctccgc catttatttt tatttattat
    11821 accacgaaga aaatgacggt attttgtttt tttccttaca atagctttaa cttatggtaa
    11881 agttaagctg aagtatttat tctttataga aaggatgttt tatatggaaa actgtatttt
    11941 ttgcaaaatc atcaatcaag aaatcccaag ctataaaata tatgaagacg acaaggtcta
    12001 tgctttttta gacatctctc aagcaacgaa aggacacaca ttagttgtgc ctaaacgtca
    12061 tgtagcagat atttttgaat atgatcccga acttgcagga gaggtttttt cgcgtgtgcc
    12121 taagattgct caagcattgg aaaaagcttt tccagaaatg aaaggtctta atatcttgaa
    12181 taacaaccgt gaactagcct atcaatctgt ttttcattca catatccatt tagtccctcg
    12241 ctacagcaaa gaggatgatt tttctattca ctttgtcaat catcaagata gttatggatc
    12301 ggaagaacta aaagctattc aagaaacaat cgtaaagcag gtgtcctgcg atgattaaaa
    12361 actttttcaa aggactgtta tttggagctg ctgctggcac tgtcggaggt ctgttattgg
    12421 cgcccaggag cgggaatgaa acaagaaaga aactgatcga agaattggaa gaagcaacaa
    12481 ctctgacaaa tgatttaaat aacagcctga atcattttaa aagcgcattg atcgaaacaa
    12541 aagaaacagc tgaatctgtg attcctgctt tccaagaagc aatcaaaaaa gatatcgaag
    12601 attttaaatt ccaagccgaa ccacgtatca cacaaatcaa tgaacaagta gaaaaactaa
    12661 aagctgatct gccagagtat cctgaacaaa tataataatg attattttct caacagattt
    12721 aactatataa gtgagaaaag tttgcgtatt tcctaggaat gtgatgaaat tttgaatttt
    12781 gtttgaaact accacgacaa cgtttttaaa ggaaatggga tatggtatag ttaatatata
    12841 aaaataataa ctaattacag gagtgcaaat tttaatgaaa aaaaaatcaa ttattttggc
    12901 agctactagt gcgttggctg ttttaactct agcagcttgt tcaggcgata ctaataaaga
    12961 tatcgcaacg atgaaaggcg gaacgatcac tgtatctgat ttctatgatg aagcaaaact
    13021 tgaatcatcg aaccaatcct tagtacaacg gatgatcatt tataaagtat tcaataacaa
    13081 atatggcgat aaagtaaccg ataagcaagt ggatgcagaa tacgacaaac aagcgaaatc
    13141 attaggtgat acttttgaat cacaattaga agctgctgga tatacgaaag atacttataa
    13201 agaatatatt cgtaacaatt tagcttttga agctggcttg aaagctcacg tagatattac
    13261 agatgacgat ttgaaaactg cttggaaatc tttccatcca gaagtagaag cacaaatcat
    13321 caaattatca agtgaagatg aagcaaaaga tgtgaaaaaa tcagcggatg atggagatga
    13381 tttctctaaa ttagccaaag acaaatcaac tgatacagag acaaaagaag atggcggtaa
    13441 agttaaattc gactcaacga caacaactat tcctgcagaa gtaaaagaag cagctttcaa
    13501 attgaaagac ggtgagatct ccgatgttat tactacaaca aatcctactt cttatgcgac
    13561 agaatactac gttgtcaaaa tggtgaaaaa ccaaaataaa ggcaatgaca tggataaata
    13621 caaagatcaa ctaaaagata tcgctactga aactaaacta agtgacaatg cattcacaac
    13681 taaagtaatc ggtgaagaat taaaagatgc aaatgtaaaa atcaaagatg atgcctttga
    13741 aaatgtgttg agtgcattca caacaacttc atcaagtacg aaagactcaa gtgaaactac
    13801 agcttcaaca aagagttctg acacaaaatc aactgattca acaaaagaat cttcaactga
    13861 agaaacaact gattcatcaa aataaaagaa aacaaaaaaa gcgaagactt atgtctcgct
    13921 ttttttgttt tgaatgaaaa aaataaattt ttgctataaa tcttttggta catagaaact
    13981 tcggttgtcc ataccgaaaa tccgatcggt gtattttcct ggttctgttt gacgaatcga
    14041 agtcaacatc atctgcatcg aagcatcgat attgtcgatt tgatgtaata tttctgcttc
    14101 cattattctt ggacgtacag gtgagccata ttctaataag ccatggtgtg ctaaaaccat
    14161 atgacgtaat acgagaacat cttcgtcagc ttcgctgatt ttcaacgcaa gacaggcttt
    14221 agttatttct tcgtcaatca ataccagatg accgatcaaa tttcctgcaa gcgtatattc
    14281 tgtcgacata ggaccggaaa gttcaatcac ctttcctaaa tcatgcaaaa tgataccagc
    14341 atacaacaat gctcgattca gctcaggata ttcatcggcg attgatttcc ctaagtgaag
    14401 cattgtcaaa gtatgataag ctaatccact ggcaaatgca tgatggtttc tttttgctgc
    14461 cggaaactca aaaaattgtt tttgatattt attaagtaga tagcgaacaa tacgattcca
    14521 atgcgcattg gtgatctcaa acaacaattg attgatttct tcttccattt cttctttctt
    14581 caaaggggct ctttccatgt ataaactagg ctctcctggc tcatctaccc ttgttaagcg
    14641 catatgaaga atctttactt gagggtttcc ttgataaacc tctcttttac cgtttagaaa
    14701 aacgactgtg ccggctgtaa atttattgat ttcctcctcc gaagcatccc agtatttacc
    14761 atcgatagta cctgaagtat cttggaaagt aaaagcaata aatttttttc cgtttttagc
    14821 gattcttaca tctgcacttt tgatcaggac aaattgttca aactgctcat cgattactaa
    14881 atcacgaatt tttttcatta tttgctcctt ctaattgttt tacgatttgg ttcatctcat
    14941 ggtaatagct taccatttct ttatcagaag aaaaacaaat tatttgatac ttttctccaa
    15001 atttagctaa aagttcagct aattgatact ttcgctgatg atcataatga agccagccat
    15061 catcaatgat gatcgggcat atcgaacggt tagcttctaa tgagaggtaa gcaaatctca
    15121 tcgccataat cagctgatct ttcgtacctg tagataactc atagatggga aattctgtat
    15181 atccgtctgt taaagtcagt gtatcatttt tcagtaatat cttctgataa tttccttttg
    15241 ttaaaatacc taaataatca cctgctgctc ttaacaactg gggtaattgt ctttcactca
    15301 tctctgttga aagatcacct aagaatgtcc cggcgatttc aagtgctccc cattcctcca
    15361 gtaattcctg taattttgct ttttgccggc tagcttgttg ataaagttcg tccaatgtgc
    15421 cgtctctttg caactcttct acttgaagtt ggagtcgttg ccgattttca cttgcttctt
    15481 tttcttcttg ctggagttgg gattgctttt ctttcagttg gcgcagtctt acagataatt
    15541 tctcttttgt cagctcttca ggaaatagtg gagtaagcat ttccgataat tcattgtatc
    15601 ttttcatctg ctgttcggct tgtttggctt tttgtaaaaa gacaggtact tccgaaggat
    15661 aagataagcc tgcttgagtc aattgttcgc tgtattcttg tatccatact ttttgtttct
    15721 ttttcaattg attgatttct tgcttcaata aagtattttg ctgatagctt ctggcaaatt
    15781 tcatctgttc catctcttgt ataaaacggg aaagcaatac cattttttct gaaattgtct
    15841 tatcttgtat cggtaaccat tctttaacaa actcaaattt ttggtcgatt tgtcgtagtt
    15901 tttcttgtat ttcctgttct cttttatgtt tccgctcaaa tacgccatat aatttctgat
    15961 aatctatgac ttcctgtcca tattctttca tgagtttgag cgtatccatc tttcctaagt
    16021 gccgatgatc cgcaaaacga tatacttctt gctgaagaat ctgtttttct tctccagttc
    16081 tcttttgttt ttcttctgct tcagctaatt gagctaaggc tagatcaagt tgtcctagtt
    16141 tttcttgcca cattcctttt atctcagaga aatcattttt agttttaatt tttggttgcc
    16201 ttttgtagaa gaaatagcca atcatgcctg caccaattag taaaaggatc actttcaagg
    16261 gcattggtac aaatatacct gcaatgatta ttgctccgcc agccatcagc atatacggtt
    16321 tagatgaaga gactggtgct atctctgact gattttgtaa taatgctgga taggtctctt
    16381 ccaagtcatt cacttctgct tcaatcatct cttgctgttc tttagctatt tggagacgca
    16441 cagaaatctg gctttctaga tcagcaatcc ttctttcttc agccagaaga tgcttccatt
    16501 cttcttcata taaaaattct tccggtaacg gatttttctg ccaatgccac ctttgttcca
    16561 aaatcttcaa ttctgaattt gtttctctag tctcaatttg taatctttgc cattcttcgt
    16621 ttaattgttc cgctgtaaat tgaagttgct ggagatcttt tagttcctgc tcctgctcta
    16681 aataaaaata atatctggct gactgctgat ccatcccgct atgcttttcc agtgttgctt
    16741 ctaattcttc caaacgctca tttgtttgct gataccgttt ggagaaagac tccagatcta
    16801 aaagaagttg ctcatcagga agacctgtag tgctgttttt tttgagagat tgccattctt
    16861 catataaaga aaaattaagt tcttgctgtc tggctaatga aaattgtttg ttcgcctctt
    16921 ttaattgtgc caataactgc tgctgttgcg actcgtattc ctgttcttgt tggatcaact
    16981 tgccaaaata agcttcctgt tcttgttttt gatgaatttt ttcttgcagc tctttccatt
    17041 catttaattt ttgattgatt agtaattttt ggcctttttt cttgaataat tgttgtgctt
    17101 tctgataata ttcttgccgc ttttggaata cttgagaact gcccgtgatg cctaaagaaa
    17161 tcagcgcatc atgtagctct ttttctctca aatgatctag ttccatcaac tgctcttgct
    17221 ggaaagtaaa tacctgctgg aataactctt tcgtcaaagg tgcgatcagt ttttctagta
    17281 actcatcact tcccacttga tcccccagcc acaccttcga ttttccgcga ttttgctgct
    17341 tgtagcgctc gacagcaaat tcaccatata caggatgttt aagccatatt ttgccgccat
    17401 aggctgagcc gtcttttggt gtataatcct tttttttacg gccttttgaa gggaaaccaa
    17461 ataatattgc ttgaataaat tgataaatcg ttgattttcc tgcttcgttt cccccataga
    17521 tcagctgatt gcctgaataa aagtcaaaac ttttttgacg ccattttcca aaagcagcaa
    17581 tctcgatcct taaaatcttc attcctgatc ctcctcaaat aaaaaatctt gattaagcag
    17641 ctgcttggct ttttcaagtg tttcttcttg atattccggc agttcgctca atattctagc
    17701 cgcttcttga tgactatata cttcatttaa aatttgttgg aaatcttgag gtgtctgata
    17761 gtattgaaat aactgttcca tcaatttagc agaagcagca agtggggtct tcgtataaaa
    17821 agtattctct atgagagaaa tacgccaaat aaagaaatca tttatcctat ctgagaattc
    17881 ctctaacaaa tagtctaata gctctcctga ttgtatccgt tccaaaacat ctgtattgag
    17941 atggtcataa tcttttaatt ttatctcaat taaactaaat ccatcctcta cttttgaaag
    18001 ctgctgacga atgtgaacaa tgacctcttt tgtcgttctt gctttcctca atgatatttc
    18061 ctttgtcttc caatagacct ctgctacttt aatcggatgt atttggcaac ttcctttact
    18121 taattcaact agtagaatag atgtagaagc ttgttccttt ttagtatgac cttgaggcgc
    18181 acctggataa acaattgttt gcttttcatt taagaccgtt ggcacatgga tatgccctaa
    18241 agcccaataa tcgtatccct tttcttgcat ctttgagggt tggaaaggag catagtttcc
    18301 tttttgagag acgccaggtt ccccgtggta caacccaata tggtaatctg tgagttctct
    18361 agaaggaaat tccatcactt tgtctttttg aatccacggt tgacgataac taaatccact
    18421 gagagagaca gtttctccac tttttatcgt tatttttttt gtctctaccg tttcggatgt
    18481 aaataaatgg acatttttag gaaaggagaa ccaataccgc tctttttgat agaaatcatg
    18541 atttccgaat attataaata caggtatatg gttcttttct aaccgtttca tctgttctga
    18601 aaaatgtttc tgtattttta atgaaggacg attttgatga aaattatctc ctgcaagtaa
    18661 aacaaaatca acgttattta ttattgcctg ttcaatgata tttgataaaa cggtcagatt
    18721 cgtcttcaat agtttttcct gtacagtttt atcaagagtt gtcagtcctt caaatgaccg
    18781 atccatatgt aaatctgccg catggataaa tttcaccatt aaaaatttcc tccttttttt
    18841 cttttatcat accattttca atgttttttt tcatcaataa ccgaaaattt gttcgtatta
    18901 tgcaaagaaa ttacgataaa tggccgactt caaaaaataa gtttattcga aaaaaagatt
    18961 gtgacatgat ttttgtcaca atcctaaata tctatgttat attcgcttcg tttgacactg
    19021 aagaagtact atttgtactg gaatttatgc tccatttttc cgtatttaga attctatgtt
    19081 ctatactacc ttaattagtt tatatggtaa gaattctttc aaatgcttga gatactccgt
    19141 ctttatcatt tgaaacagtg acataatcag cttttttcat gatttcttga tgactatttc
    19201 ccattgcaaa actaatatct gcactttcaa acatagataa gtcatttaaa ctatctccga
    19261 tcgcgattct caaaggtttc tttaaattgt agattgattc caaacgatca aacaccacac
    19321 ctttagatac attttgaggt acgatttcaa tatttacagg tgccgattca gtaacagaca
    19381 catttagcat ctttttcaaa tggttaaata tattagcttt caattgacga ttaggaataa
    19441 aaataaaaaa tttgtaaatg ctgatagcat ccttttctat aatttcctca atattattat
    19501 aagggattga acaaatagat ttttca
//
LOCUS       DACEMO010000031        19455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEMO010000031 DACEMO010000000
VERSION     DACEMO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.031"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..3658
                     /locus_tag="IW728_000868"
     CDS             131..3658
                     /locus_tag="IW728_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1024245.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="IW728_000869"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="IW728_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1024246.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="IW728_000870"
     CDS             7446..7787
                     /locus_tag="IW728_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1024247.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="IW728_000871"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="IW728_000871"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1024248.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="IW728_000872"
     CDS             9144..11564
                     /locus_tag="IW728_000872"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1024249.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IW728_000873"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IW728_000873"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1024250.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="IW728_000874"
     CDS             12998..13735
                     /locus_tag="IW728_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024251.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="IW728_000875"
     CDS             13749..14564
                     /locus_tag="IW728_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1024252.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="IW728_000876"
     CDS             14577..15224
                     /locus_tag="IW728_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1024253.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="IW728_000877"
     CDS             15240..15896
                     /locus_tag="IW728_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1024254.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="IW728_000878"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="IW728_000878"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1024255.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="IW728_000879"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="IW728_000879"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1024256.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="IW728_000880"
     CDS             18238..18756
                     /locus_tag="IW728_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1024257.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /locus_tag="IW728_000881"
     CDS             18863..19363
                     /locus_tag="IW728_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1024258.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6542 a         3341 c         4177 g         5395 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaagaaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagc
//
LOCUS       DACEMO010000032        19367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEMO010000032 DACEMO010000000
VERSION     DACEMO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.032"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(375..1328)
                     /locus_tag="IW728_000882"
     CDS             complement(375..1328)
                     /locus_tag="IW728_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1024259.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1351..2106)
                     /locus_tag="IW728_000883"
     CDS             complement(1351..2106)
                     /locus_tag="IW728_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1024260.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2103..3062)
                     /locus_tag="IW728_000884"
     CDS             complement(2103..3062)
                     /locus_tag="IW728_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1024261.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3055..4005)
                     /locus_tag="IW728_000885"
     CDS             complement(3055..4005)
                     /locus_tag="IW728_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1024262.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4155..4607
                     /locus_tag="IW728_000886"
     CDS             4155..4607
                     /locus_tag="IW728_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1024263.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="IW728_000887"
     CDS             complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="IW728_000887"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1024264.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="IW728_000888"
     CDS             complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="IW728_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1024265.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8159..8968)
                     /locus_tag="IW728_000889"
     CDS             complement(8159..8968)
                     /locus_tag="IW728_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1024266.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8977..12558)
                     /gene="smc"
                     /locus_tag="IW728_000890"
     CDS             complement(8977..12558)
                     /gene="smc"
                     /locus_tag="IW728_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1024267.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12578..13264)
                     /locus_tag="IW728_000891"
     CDS             complement(12578..13264)
                     /locus_tag="IW728_000891"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1024268.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13612..15393)
                     /locus_tag="IW728_000892"
     CDS             complement(13612..15393)
                     /locus_tag="IW728_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1024269.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15419..16333)
                     /locus_tag="IW728_000893"
     CDS             complement(15419..16333)
                     /locus_tag="IW728_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024270.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16360..17322)
                     /locus_tag="IW728_000894"
     CDS             complement(16360..17322)
                     /locus_tag="IW728_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024271.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17322..18266)
                     /locus_tag="IW728_000895"
     CDS             complement(17322..18266)
                     /locus_tag="IW728_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024272.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18267..19271)
                     /locus_tag="IW728_000896"
     CDS             complement(18267..19271)
                     /locus_tag="IW728_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024273.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5334 a         4195 c         3429 g         6409 t
ORIGIN      
        1 cttattgccg agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa
       61 aatatctaaa tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga
      121 ggcgatcttg atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat
      181 caacccgtat gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa
      241 accatccgta aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat
      301 aggctcttgc tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc
      361 ctctgttatt tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat
      421 tctaaaccgc ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt
      481 ttaccagcat tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct
      541 ccaccaatag cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca
      601 tattcataag aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca
      661 aagccaaata aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc
      721 ccttcattca caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc
      781 tgatcgattg cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag
      841 atctccccta aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa
      901 gaaaggaaaa tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca
      961 gaaatgatga ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt
     1021 ccacctgctg attctacttt tttataggat gaaagatact caggaaggct gcttgtagct
     1081 gctccaacta ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg
     1141 aatacgacga ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt
     1201 atcgtcaatg tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg
     1261 ttgctatttc cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat
     1321 tttttcatct cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc
     1381 aatctcacaa atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat
     1441 ttcttgggtc gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc
     1501 ataacttgca gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg
     1561 tactaaattt tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa
     1621 aggttcatcc agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg
     1681 ttggagttga ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaat
     1741 gtgttccagt gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata
     1801 aggaaatcgt ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga
     1861 ttgtcgtagg atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt
     1921 atggtctaaa tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac
     1981 cgttgacttt ccagcaccat ttggaccgat aaaagcagtc aacttctttt caggaatcgt
     2041 taactgtaca tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc
     2101 aatcatagga tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca
     2161 aattcaacga cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt
     2221 cctcctgtca gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga
     2281 taagtagaaa agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga
     2341 cccacaagag ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca
     2401 aaacgatctg tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct
     2461 agcacatgtc gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc
     2521 aaaagatact gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca
     2581 tattcattcg gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct
     2641 aaaatcatcc ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag
     2701 aaaaataaca atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta
     2761 cctgtctgct gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg
     2821 acatataacg actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc
     2881 tgaaagctga ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc
     2941 ttactcctca atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt
     3001 cctgtaacaa ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc
     3061 atatcgtcgt ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat
     3121 cacactgact ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac
     3181 taagaacaaa ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata
     3241 tattctagag acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat
     3301 caacatgact gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta
     3361 atctattccc agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc
     3421 aaatagccaa accaagagca acaatggaac aacagcatac aataactcat aatttcctcg
     3481 tatcacagtc gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg
     3541 ataagcaaaa aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg
     3601 aagcatcaat tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc
     3661 tgcaaaagca aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa
     3721 aatcatagcc actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga
     3781 aacaaattta ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc
     3841 cacaaggatc aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg
     3901 cccatctaac tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat
     3961 actacttaaa ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct
     4021 aattgagaat gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt
     4081 tcatatgttt taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa
     4141 ggagggtatt ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg
     4201 gtacaccttt agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct
     4261 gctatcttag tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa
     4321 aagaactcaa aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg
     4381 tccaaaacca atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg
     4441 attacgtcac tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa
     4501 atgaacctgt gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa
     4561 aagaaaaacc aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac
     4621 ataaattttt acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt
     4681 caaaagcttt ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa
     4741 tcttctgcct tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc
     4801 ttcgatgatc ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat
     4861 caaatcgata ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt
     4921 gtaagcttca gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga
     4981 gttctccctt agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg
     5041 ttctttcaac atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg
     5101 attcgttcca cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat
     5161 actgtcacca ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat
     5221 cacaaagaaa cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc
     5281 acgaaaagca atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat
     5341 cgtgatacct aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa
     5401 tctcggataa gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg
     5461 gaacccagct tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac
     5521 gatatagctg tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa
     5581 ttgcagacgc aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat
     5641 ggcatcgaac ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata
     5701 gggacgttca tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg
     5761 actcgatagc tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat
     5821 ttgagtcaat gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat
     5881 ttgcttcatt ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg
     5941 ttgttctagt gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc
     6001 ttctttttct tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc
     6061 tggattgata cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg
     6121 aacacctgtt gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata
     6181 aaattctttc tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata
     6241 ctgtttcata gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact
     6301 gttgcgaata ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc
     6361 actcattggt gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct
     6421 cgctgttttc aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg
     6481 gctgaacaat gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac
     6541 catctgtttt cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa
     6601 attttttaat tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact
     6661 taatggccag atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc
     6721 gagatactga aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt
     6781 ttcgctaaaa tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg
     6841 atcggttttt gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa
     6901 tcgagcttgg tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat
     6961 cattaagtga cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa
     7021 cgaaattgtt tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc
     7081 tatgttaaaa acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt
     7141 agacatgggt tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg
     7201 atcaaacggc tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg
     7261 caattcatta cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa
     7321 aacaagaccg gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg
     7381 accagtggtt gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg
     7441 gattactcgt tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc
     7501 tgcagtatca actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa
     7561 tacgacagct gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc
     7621 gccccagaca actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa
     7681 tactttttta ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc
     7741 tacaccatta acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc
     7801 atttacttca ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt
     7861 ttggacttct gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt
     7921 gattcgtatc gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc
     7981 aaaaaagtct tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa
     8041 tgttttacgc gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt
     8101 tttctcttcg ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct
     8161 actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca
     8221 cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag
     8281 catattggat catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt
     8341 tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca
     8401 taaattccaa aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg
     8461 gtatttcttt gattttagga tctaattctt cttggtcata agcgattact gccttattaa
     8521 gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca
     8581 acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag
     8641 acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg
     8701 atacttccat cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc
     8761 cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg
     8821 ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt
     8881 gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt
     8941 ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt
     9001 cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg
     9061 catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac
     9121 gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc
     9181 ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat
     9241 aatggaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag
     9301 actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc
     9361 agttggttcc gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat
     9421 tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc
     9481 catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt
     9541 tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac
     9601 tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat
     9661 atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg
     9721 cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt
     9781 ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag
     9841 cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt
     9901 taatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac
     9961 ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg
    10021 ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa
    10081 ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc
    10141 tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt
    10201 ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta
    10261 atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc
    10321 aaaacgttca agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa
    10381 acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc
    10441 ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa
    10501 ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg
    10561 tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac
    10621 cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc
    10681 taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac
    10741 ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc
    10801 cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg
    10861 ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg
    10921 ggctgcccca cctaaagctg cttcgatagc taacgtgaaa tctgcaggaa catcgatcag
    10981 ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac
    11041 cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg
    11101 tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc
    11161 ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga
    11221 tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac
    11281 tgatgcttct acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat
    11341 atattgacgt tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc
    11401 aacatattga tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa
    11461 ttcttgctgt gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc
    11521 tgctgctttc atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc
    11581 tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa
    11641 aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg
    11701 atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga
    11761 ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg
    11821 tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac
    11881 atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc
    11941 ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt
    12001 gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac
    12061 accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc
    12121 tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa
    12181 ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt
    12241 ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact
    12301 attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc
    12361 aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac
    12421 ccagcggata gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt
    12481 gacgctattt tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc
    12541 aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt
    12601 ccgccgcagc ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat
    12661 catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca
    12721 atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat
    12781 gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga
    12841 atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata
    12901 ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca
    12961 aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga
    13021 tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat
    13081 actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg
    13141 ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca
    13201 aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt
    13261 ccattttcca cattcctttt atggatattt tttcgatact tccttattat actggttttt
    13321 tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg
    13381 acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg
    13441 gctttccata tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt
    13501 ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa
    13561 atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg
    13621 tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca
    13681 gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa
    13741 tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc
    13801 aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg
    13861 agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt
    13921 attccaagca gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa
    13981 agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca
    14041 ttgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa
    14101 gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt
    14161 gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat
    14221 ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa
    14281 acctgcatag aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg
    14341 taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt
    14401 ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt
    14461 atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc
    14521 atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat
    14581 ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc
    14641 tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg
    14701 atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata
    14761 tgaccagata ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc
    14821 aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata
    14881 ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt
    14941 attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga
    15001 ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag
    15061 cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg
    15121 agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc
    15181 tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc
    15241 attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga
    15301 gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat
    15361 cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact
    15421 agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac
    15481 tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta
    15541 agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca
    15601 agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg
    15661 tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa
    15721 aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg
    15781 tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa
    15841 aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg
    15901 gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt
    15961 agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc
    16021 cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt
    16081 catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt
    16141 tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga
    16201 tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa
    16261 attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt
    16321 ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg
    16381 gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa
    16441 aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag
    16501 ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga
    16561 agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg
    16621 actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga
    16681 tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc
    16741 ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc
    16801 accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa
    16861 aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta
    16921 gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg
    16981 cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat
    17041 ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc
    17101 ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc
    17161 tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa
    17221 tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg
    17281 cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct
    17341 ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca
    17401 tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg
    17461 tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag
    17521 atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg
    17581 tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat
    17641 aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga
    17701 acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca
    17761 acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta
    17821 tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct
    17881 tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc
    17941 tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa
    18001 cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg
    18061 gctgttacct tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac
    18121 tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca
    18181 tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg
    18241 tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa
    18301 tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct
    18361 tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga
    18421 ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt
    18481 ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac
    18541 agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca
    18601 gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct
    18661 tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc
    18721 gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca
    18781 cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta
    18841 ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc
    18901 tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat
    18961 gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc
    19021 gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg
    19081 ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat
    19141 tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca
    19201 tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg
    19261 ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa
    19321 ggacattaca cagaataaat cgttttataa caaaggttat tcttatc
//
LOCUS       DACEMO010000033        18295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEMO010000033 DACEMO010000000
VERSION     DACEMO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.033"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(73..1965)
                     /locus_tag="IW728_000897"
     CDS             complement(73..1965)
                     /locus_tag="IW728_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024274.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1962..3689)
                     /locus_tag="IW728_000898"
     CDS             complement(1962..3689)
                     /locus_tag="IW728_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024275.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3705..4355)
                     /locus_tag="IW728_000899"
     CDS             complement(3705..4355)
                     /locus_tag="IW728_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1024276.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4559..4855
                     /locus_tag="IW728_000900"
     CDS             4559..4855
                     /locus_tag="IW728_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1024277.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5047..5667
                     /locus_tag="IW728_000901"
     CDS             5047..5667
                     /locus_tag="IW728_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1024278.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5759..6925)
                     /locus_tag="IW728_000902"
     CDS             complement(5759..6925)
                     /locus_tag="IW728_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1024279.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6937..7290)
                     /locus_tag="IW728_000903"
     CDS             complement(6937..7290)
                     /locus_tag="IW728_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1024280.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7418..8440
                     /locus_tag="IW728_000904"
     CDS             7418..8440
                     /locus_tag="IW728_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1024281.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8879..9313)
                     /locus_tag="IW728_000905"
     CDS             complement(8879..9313)
                     /locus_tag="IW728_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024282.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9423..11282)
                     /locus_tag="IW728_000906"
     CDS             complement(9423..11282)
                     /locus_tag="IW728_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1024283.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11400..12559)
                     /locus_tag="IW728_000907"
                     /pseudo
     CDS             complement(11400..12559)
                     /locus_tag="IW728_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(12866..14290)
                     /gene="araA"
                     /locus_tag="IW728_000908"
     CDS             complement(12866..14290)
                     /gene="araA"
                     /locus_tag="IW728_000908"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1024284.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14306..15004)
                     /locus_tag="IW728_000909"
     CDS             complement(14306..15004)
                     /locus_tag="IW728_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1024285.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14961..16622)
                     /locus_tag="IW728_000910"
     CDS             complement(14961..16622)
                     /locus_tag="IW728_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1024286.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16840..18240
                     /locus_tag="IW728_000911"
     CDS             16840..18240
                     /locus_tag="IW728_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1024287.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
BASE COUNT         5217 a         3861 c         3297 g         5920 t
ORIGIN      
        1 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
       61 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      121 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      181 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      241 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      301 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      361 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      421 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      481 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      541 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      601 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      661 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      721 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
      781 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
      841 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
      901 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
      961 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1021 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1081 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1141 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1201 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1261 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1321 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1381 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1441 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1501 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1561 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1621 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1681 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1741 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     1801 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     1861 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     1921 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     1981 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2041 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2101 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2161 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2221 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2281 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2341 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2401 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2461 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2521 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2581 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2641 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2701 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     2761 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     2821 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     2881 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     2941 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3001 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3061 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3121 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3181 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3241 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3301 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3361 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3421 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3481 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3541 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3601 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3661 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3721 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     3781 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     3841 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     3901 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     3961 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4021 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4081 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4141 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4201 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4261 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4321 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4381 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4441 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4501 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4561 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4621 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4681 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4741 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     4801 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     4861 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     4921 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     4981 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5041 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5101 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5161 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5221 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5281 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5341 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5401 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5461 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5521 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5581 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5641 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5701 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     5761 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     5821 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     5881 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     5941 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6001 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6061 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6121 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6181 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6241 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6301 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6361 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6421 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6481 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6541 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6601 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6661 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6721 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     6781 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     6841 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     6901 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     6961 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7021 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7081 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7141 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7201 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7261 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7321 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7381 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7441 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7501 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7561 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7621 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7681 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7741 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     7801 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     7861 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     7921 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     7981 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8041 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8101 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8161 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8221 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8281 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8341 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8401 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8461 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8521 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8581 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8641 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8701 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     8761 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     8821 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     8881 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     8941 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9001 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9061 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9121 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9181 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9241 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9301 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9361 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9421 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9481 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9541 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9601 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9661 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9721 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
     9781 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
     9841 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
     9901 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
     9961 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10021 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10081 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10141 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10201 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10261 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10321 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10381 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10441 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10501 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10561 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10621 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10681 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10741 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    10801 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    10861 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    10921 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    10981 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11041 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11101 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11161 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11221 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11281 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11341 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11401 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11461 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11521 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11581 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11641 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11701 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    11761 gtcgatcccc tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg
    11821 gaaaatcagt caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt
    11881 tatcaacaat ttcacttctt cctacattga taagtacagc atcttttttc attttttcta
    11941 tattcgcctg atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt
    12001 aatccgaggt agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat
    12061 cgcggataga ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg
    12121 tgcctatcgc tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat
    12181 aagcacttct tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca
    12241 tacgacttgc ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt
    12301 ttactgcatt agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt
    12361 tttcaacgtt gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg
    12421 taagccattc atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat
    12481 ctattatttt gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa
    12541 tcaaatgaac gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca
    12601 attagacaac tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta
    12661 ggctgagtga taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc
    12721 aaaatattga gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa
    12781 gactaaattt caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac
    12841 taagtggctt tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt
    12901 ttgcccacgt ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc
    12961 cacctgcctg aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca
    13021 cacgtgcaac aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga
    13081 tcagcagttt ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt
    13141 tcccatcaaa tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa
    13201 cgattcttgg tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga
    13261 ttgcttcttg gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc
    13321 ctcgagccat gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag
    13381 caaaaccgta tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc
    13441 catacaggtc ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc
    13501 gaataccgat ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat
    13561 aatcaccata gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt
    13621 cgtctgatac gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc
    13681 aaccaaattg aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt
    13741 tgtcaccaaa acgagccact tttattccaa aactttcatt ataggctact gcaacatcca
    13801 tccaatcagc aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat
    13861 tttgtttatt taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat
    13921 ttaaattcat gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag
    13981 ctaaatgaag caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg
    14041 agaaagtatg catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca
    14101 tgatttttgt gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca
    14161 attggccatt ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca
    14221 gtgcttcttc cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa
    14281 tattcaacat aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt
    14341 gttttcgtaa ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga
    14401 gctgtctggt aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg
    14461 cactttgcgc atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa
    14521 ttgctaaggg atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat
    14581 attcgtaccc gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat
    14641 aaaaagtatc tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga
    14701 tcgtcgccca tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata
    14761 aaacagcatg agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt
    14821 ccaagtccac aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt
    14881 tgataacgaa atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga
    14941 cgagtccgtg tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca
    15001 gcattttcta tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc
    15061 ccctcacggt aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg
    15121 atggtgattc cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc
    15181 ccatgtcgat ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg
    15241 gctgtgtcca tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact
    15301 tctggtgttt taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat
    15361 ttcaatatat ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg
    15421 aaattcgcta aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt
    15481 actcctgtaa tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta
    15541 tcgccttcta gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata
    15601 cccaaagaat tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag
    15661 ttattcgtat gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc
    15721 ggatggacat tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc
    15781 gaaatgttcc ctgtcccttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc
    15841 ccttctggcg gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa
    15901 gcccctgatt cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg
    15961 tgatccaagt caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt
    16021 tcttgatggt aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg
    16081 cctagtactt tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa
    16141 ttaatttctt tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc
    16201 catctctgcg gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg
    16261 attgtatttc gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga
    16321 taaccgtgca tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag
    16381 atttcgaata cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct
    16441 tctaatgaat aggtccagat accattttct agtttgtttt cccactcata actaccagcg
    16501 gcaataggag taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca
    16561 atccctaaag atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc
    16621 ataatgattt aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct
    16681 tacaatgatt attgtatact tttgtacgta catgtcaagc agttatttac aagaatacaa
    16741 taaaaaacac ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga
    16801 tcaaccgcct tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt
    16861 tcctcatcgt ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt
    16921 ggtgttatga ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct
    16981 tctttgatag gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta
    17041 tctggtcttc tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt
    17101 tttatggctg gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt
    17161 gcagcaagga ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat
    17221 atgtctgaaa tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg
    17281 attgcttctg gcatgctcct ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa
    17341 acgatggctt ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc
    17401 ggtgttttag ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag
    17461 gcaaaaagag tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa
    17521 atccagctga atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta
    17581 gaaaaatatc gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa
    17641 ggagctaatg ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca
    17701 gcgagcgatg cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg
    17761 ctctttttgg ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc
    17821 gtgatgggac tttctttcat cctaccagct gttttaggaa ctgtactgga tgctcataca
    17881 aattcgttgt tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg
    17941 gctccactga catgggtaat cgtaggcgaa atattcccac tagctgtccg cgggcgagca
    18001 tctggcctag cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct
    18061 gtgatgactg cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt
    18121 ttaggggttt tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa
    18181 atcgaacaaa gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa
    18241 agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agttt
//
LOCUS       DACEMO010000034        18291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEMO010000034 DACEMO010000000
VERSION     DACEMO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.034"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(109..786)
                     /gene="phoU"
                     /locus_tag="IW728_000912"
     CDS             complement(109..786)
                     /gene="phoU"
                     /locus_tag="IW728_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1024288.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(799..1557)
                     /locus_tag="IW728_000913"
     CDS             complement(799..1557)
                     /locus_tag="IW728_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024289.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1573..2379)
                     /gene="pstB"
                     /locus_tag="IW728_000914"
     CDS             complement(1573..2379)
                     /gene="pstB"
                     /locus_tag="IW728_000914"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024290.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2394..3278)
                     /gene="pstA"
                     /locus_tag="IW728_000915"
     CDS             complement(2394..3278)
                     /gene="pstA"
                     /locus_tag="IW728_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1024291.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3278..4198)
                     /gene="pstC"
                     /locus_tag="IW728_000916"
     CDS             complement(3278..4198)
                     /gene="pstC"
                     /locus_tag="IW728_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1024292.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4313..5170)
                     /locus_tag="IW728_000917"
     CDS             complement(4313..5170)
                     /locus_tag="IW728_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1024293.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5507..6955)
                     /gene="cls"
                     /locus_tag="IW728_000918"
     CDS             complement(5507..6955)
                     /gene="cls"
                     /locus_tag="IW728_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1024294.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(7107..8000)
                     /locus_tag="IW728_000919"
     CDS             complement(7107..8000)
                     /locus_tag="IW728_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024295.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(7984..8670)
                     /gene="ftsE"
                     /locus_tag="IW728_000920"
     CDS             complement(7984..8670)
                     /gene="ftsE"
                     /locus_tag="IW728_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1024296.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(8775..9876)
                     /gene="prfB"
                     /locus_tag="IW728_000921"
     CDS             complement(join(8775..9812,9814..9876))
                     /gene="prfB"
                     /locus_tag="IW728_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1024297.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEKEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(10002..12536)
                     /gene="secA"
                     /locus_tag="IW728_000922"
     CDS             complement(10002..12536)
                     /gene="secA"
                     /locus_tag="IW728_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1024298.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(12758..13309)
                     /gene="raiA"
                     /locus_tag="IW728_000923"
     CDS             complement(12758..13309)
                     /gene="raiA"
                     /locus_tag="IW728_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1024299.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(13437..14156)
                     /locus_tag="IW728_000924"
     CDS             complement(13437..14156)
                     /locus_tag="IW728_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1024300.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(14153..15466)
                     /locus_tag="IW728_000925"
     CDS             complement(14153..15466)
                     /locus_tag="IW728_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1024301.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(15547..15867)
                     /locus_tag="IW728_000926"
     CDS             complement(15547..15867)
                     /locus_tag="IW728_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1024302.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(15975..16262)
                     /locus_tag="IW728_000927"
     CDS             complement(15975..16262)
                     /locus_tag="IW728_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024303.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            16389..17033
                     /locus_tag="IW728_000928"
     CDS             16389..17033
                     /locus_tag="IW728_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1024304.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(17092..17799)
                     /locus_tag="IW728_000929"
     CDS             complement(17092..17799)
                     /locus_tag="IW728_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1024305.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
BASE COUNT         5110 a         3995 c         3188 g         5998 t
ORIGIN      
        1 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
       61 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      121 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      181 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      241 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      301 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      361 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      421 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      481 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      541 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      601 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      661 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      721 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      781 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      841 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
      901 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
      961 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1021 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1081 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1141 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1201 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1261 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1321 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1381 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1441 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1501 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1561 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1621 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1681 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1741 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1801 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1861 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     1921 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     1981 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2041 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2101 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2161 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2221 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2281 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2341 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2401 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2461 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2521 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2581 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2641 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2701 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2761 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2821 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     2881 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     2941 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3001 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3061 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3121 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3181 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3241 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3301 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3361 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3421 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3481 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3541 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3601 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3661 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3721 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3781 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3841 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     3901 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     3961 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4021 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4081 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4141 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4201 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4261 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4321 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4381 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4441 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4501 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4561 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4621 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4681 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4741 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4801 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4861 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     4921 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     4981 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5041 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5101 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5161 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5221 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5281 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5341 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5401 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5461 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5521 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5581 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5641 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5701 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5761 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5821 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     5881 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     5941 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6001 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6061 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6121 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6181 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6241 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6301 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6361 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6421 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6481 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6541 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6601 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6661 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6721 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6781 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6841 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     6901 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     6961 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7021 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7081 tttctcattt tatttatatt gtggtattag aatttcaaaa aacgtctcat agaaatgatc
     7141 gagccaagtg aaccaatgat cacaccaatg ccgaccatta aaagattgat tttccaaacg
     7201 atattttgtg gacgaatcaa tgaataatgg gaacgtaata atgatggatt gatcaattgg
     7261 aaaaaccatg cataaccaaa tgtgatgatc aaaatcggaa gaatcgcacc gatcagaccg
     7321 atccagccac cttcaaggaa aaacggccag cgaatatatc cattttttgc accgactaaa
     7381 cgcatgattt gaatttcacg ctgtctagac aaaatcgtga tccgaatcgt gttcgaaatg
     7441 agaaacatcg ctacgaacag cagcaatgcc gctgcaacta atccccatgt ccgaactgca
     7501 ccagcgattt ggaaaatccg atctgaggaa gctccaccgt aatcagaacg agaaacattc
     7561 ttcaaatctg ctgcttcttc tgctacttgt tttgtataag aaggatcagt agcacttact
     7621 acatacacat catataacgg gttattgtca ccttcaaaca atttccagga atcacccatt
     7681 tcgttttgga tttttttcaa ttcctgttct ttgcttgaaa attcaacttt tttaacatga
     7741 tctaagtcat tcaattcttt ttctagcttt ttcatctctt cgtcttttgt accgatatct
     7801 acgaatacag atacatcaac gtttccttct atatcctgag ctaatttagt tgcattcatg
     7861 ataactgcca tgaatacacc gactaaagtc aacgtgatcg ttaccgcact gactgaagca
     7921 actgtcatcc agccgttacg tttcaaactc ttgatacttt ctaataaatg gcggaaaaag
     7981 gttctaatca tcgtatccat attccccttc cgcttgatct cgaataatgc ggccgttttc
     8041 gatagcaatt actcggtgcc gaatcgtatt tacgatcgta ctgttatgtg tagccatcac
     8101 gacagtagtt ccttgggcat tgatccgatc taataatttc atgatttccc atgagttttc
     8161 tggatccaga tttcctgtcg gttcatccgc gataagtact ttaggcgtat tcacgattgc
     8221 ccgagcaata gagacacgtt gctgttcccc accggataat tcgctaggaa agactcgaac
     8281 tttgtgtttc aaaccaacaa ggtccagcac ttccattact cgttttttga tatctcgagg
     8341 ttttctgccg ataacttgca tagcgtacgc aacattttca tatactgttt ttcttggtaa
     8401 taatttatag tcttggaaca ccacgccgat ttctctgcgt agataaggaa cttcgcggtt
     8461 tttgattgct attagatcat gacctgcgac gttcaaagtt ccttttgaag ctttttcttc
     8521 tcgatacatc aatttgatga atgtagattt accggctcca gacggaccaa ccacatagac
     8581 aaactctcct tgttcgatga caatcgagag attgcggata gcggtcgtac catttgagta
     8641 cttcttcatt acatccttca tttcaatcat ggcgttctct ccatatcttt ttcagtcttt
     8701 agctattata gcatggcaat gtttcaacat gcttgcaaaa tcttacaatt ttatttcaat
     8761 ttaacaatga gttcttattg tagatgcatt tttaaatagc cgtcaataaa taaatcaagg
     8821 tcaccgtcca taactgcttg gacatttcct gtctctacgt ttgtccgatg gtcttttacc
     8881 atagaatagg gatggaaaac ataagaacgg atctgtgaac cccagccgat ttctttttgt
     8941 tctcctcgaa gtgcagctgc ttctttttct ttcttttcca cttctaattg ataaagctta
     9001 gcttttaaca tccccatcgc ttgctcacgg tttttcaatt gtgagcgttg tgcctgacta
     9061 gctactactg ttcctgtagg caagtgagtg atccgtacag cagattctgt tttattgatg
     9121 tgctgaccac ctgcaccact tgctcggtac gtatcgattt tcaaatcatc tggattgatt
     9181 tctatatcga tcgtttcatc aagctctggc atcacatcta ccgaacaaaa agaagtatga
     9241 cgtcgtttcg ctgagtcaaa aggcgaaatc cgtacaaggc gatgaacacc tttttctgat
     9301 tttagataac caaaagcgtt atgtccttta atcagcaggg tcacactctt gatccctgct
     9361 tcatcacctg cttgataatc tagtgtttct acttgatagc catgctgctc tgcccaacgt
     9421 gtatacatac gtaaaagcat gctgccccag tcttgggact ccgttccgcc agcccctgga
     9481 tgcaattcga cgatcgcatt gtttcgatca taaggtccgt ctaacagcat agataattca
     9541 tacgtcttca ttttttcatc taatgcattg atacgtgttt ctgcttcttc ttgcattgct
     9601 gcatcaggtt cttcttgcag catctccacc atcaattcga tttcttctag ttcttcggca
     9661 agagaatgga attggtcata ggtttcttta ttagcgttat tttcgctgat cactttttgt
     9721 gcggcttcgc tgtcatccca aaaaccaggt tcagccattt ttgcttctgc ttccgcgatg
     9781 tcttcttcca gctgatctaa gtcaaagaga cctcctgaaa cttgtgacct tttcctgcat
     9841 ttctgtcagg agatttcgaa tttcggatat ttccatgttt tttccttctt tcactatact
     9901 tttttcatta tatctagtct aacagaatca gcaaataaca gaaagaaggt gagacgaatt
     9961 cttaaaaaaa gaatttgtct caccaatcct gctgtcagaa attagtttgc attttttcca
    10021 tgacagttct tatatttttt cccgcttcca caaggacaag gatcatttct tccgaccttg
    10081 ttcacatgga ttggctgttt tttcgcgcta gtattactat tttctttggc atctccgtct
    10141 tctgagtgag aagctgttcc ttgagaaacc tgttcacgct gtacgttttg acggatctca
    10201 gctttcataa agattcgagt gacttcatac tcgatggcgc ctaccatatc ttcaaacatc
    10261 ttatagcctt ctgtttgata ttcgactaat ggattgttct gtccataagc acgtaagccg
    10321 attgactgac gtaattgatc cattgcatca atatggtctg tccatttaga gtccacaaca
    10381 cgcaagatca cgactttttc aaattccaac agttgctctg gaccatttag ttgagccgct
    10441 ttggtattaa atacttcttg tgctcgttcg tttagatact ctttgatctc ctgagatgtt
    10501 tttccttcga tatctgcaag agtaatacta tcttcatgga caagagtact gccggcaaaa
    10561 tcgatgatac tttccaaatt ccaattttct ttctctaatt gtgtatgcgc atctactaca
    10621 cgactgattg tccgtttgac catgttcatt agtggcttgg ataagctttt ttcttccatg
    10681 attacttctt gtcgctgttt atagattact tcacgttgtt cacgcatcac atcatcgtat
    10741 tgtaagacgt ttttacgtgt gtcatagtta tttccttcta cacgtttttg tgcagattct
    10801 acttgtttcg tcagcatctt gctttggata acagcatctt cctcaccgat cttcatccga
    10861 tctaagaaag ctttgattcg ttctgaaccg aatcgtttca tcaaatcatc ttccaatgac
    10921 aagtagaatt gtgacatacc agggtctcct tgacgtcctg cacgtcctcg aagctggtta
    10981 tcgatacgac gagattcatg tcgttctgtc ccgataactg ctagaccacc aagttctctt
    11041 accccaagtc ctaacttgat atctgttccc cgaccagcca tgttggtagc aatcgtcaca
    11101 gcaccttttt gaccagcatt catgatgatc tctgcttctt taaagtgatt tttcgcattc
    11161 agcacttcat gaggaatacg ttcttgattc agcatatctg ataataactc agatgtttca
    11221 accgcaacag ttccgactaa cacaggttga cctttgcggt agcgatcttt gatatcttgc
    11281 acgacagcgt ggaacttgct ttgtaatgtt gggtaaagta aatctggacg atcatcacgg
    11341 ataaccggac gatttgtcgg aatctggtat acttggatgt tataaatttc tctgaattct
    11401 tcttcttctg ttttagctgt accagtcatt cccgcaagtt tcttgtacat acggaaatag
    11461 ttctggaaag tgatcgtcgc catggttttc gtttcgtctt cgatctctac gccttcttta
    11521 gcttcgattg cttggtgaag tccatctgaa taacggcgtc catccatgat ccgaccagtg
    11581 aactggtcaa caatcagtac ttttccgtct tggacaacat aatcgatatc taaaagcatg
    11641 atatagttgg ctctcaatgc ttgatccaag tgatgggtca atgcagtatt ttcaatgtcg
    11701 tataagttat ccaaaccgaa tgtttcttct gctttttcga tccctgcttc ggttaaactg
    11761 attgtttttg attgtacatc gattttataa tcttcttctt ctttcaaacg tttcacgaag
    11821 ttgtctgctc ttgtatataa agctgtggat ttttctgctt gccctgaaat gatcagcggc
    11881 gttctcgctt catcaatcaa gattgagtca acttcatcga caatggcata attcagcgga
    11941 cgttgtacca tttgatggcg gtaaacgacc atattgtctc tcaagtagtc aaatcctaat
    12001 tcattattcg tactataagt gatatcacaa ttataagcct cgcgtttttc atccgaagat
    12061 tttgagttga tattcaaccc cactgttaat cctaagaaat tataaagttc gcccatttcc
    12121 gtcgagtcac gggttgctaa atattcattg acggtaacta cgtgtacccc ttcgccagtc
    12181 agagcattta gatatactgg catcgttgcg gtcaaggttt taccttcacc agtacgcatt
    12241 tcagggatat ttccatcgtg aagcacgatt ccacccatca attgtacgtg gtatggatat
    12301 aaccccaata cacgttttgc tgcttctcgg acgactgcaa aagcttccgg aagtaattga
    12361 tctaatgttt ctcctttttg gtatctgccc ttgaattcag cagtttttgc ctgtaattgt
    12421 tcatcagtta atgctgccat ttggtcagcg tatgcttcaa ctttttttgc aatgctatct
    12481 aaacgtttta gttctttctt atcgttttcg ataagtgaac gaatcaaatt ggccatgcgt
    12541 tgtgtaatct ccttttcctt ctgtgtatta tccagactat gtagacttta cttttcattt
    12601 tatcattagt tatagataat tgaaatatct taagcgtatt aaagtaagaa cttcataatt
    12661 tattcatatt tcatctaaaa gaggaggaaa ctcaaaaaaa cttgcggcag ctgtcagtag
    12721 ctgctgcaag ttttcgttgt tatttaagta aggctgatca gtctgtttca atcagtccgt
    12781 attttccatc tttacggcgg taaacgatac ttgtaccatt tgtttctgca tcttcaaaaa
    12841 taaagaagtt atgtcccagc atgttcattt gtaagactgc ttcttcacta tccattggtt
    12901 tcaatgaaag gcgtttcgta cgtacgatat ctaattcaga atcattttcg ttttcgtcct
    12961 cttcattgaa taaagcagca gcatctgcag tattcatgcc cgtttcacgg gatttacgat
    13021 taatttttgt tttgaatttg cggatttggc gttccaattt atcaacaacc aaatcgatac
    13081 ttgcgtataa atctggagaa gtttcttcgg cacgtaatac aagataaggt aatgggatcg
    13141 ttacttccac ttttgctgtt ttttctgtat aaacttttaa gttcacatgc gccgttgcat
    13201 cgggtgcgtc actgaaatag cgttctagtt tgcctacttt tttctcaacg tagtcgcgaa
    13261 tcgcttcagt tacttcgata ttttcgccgc gtacattata tctaaacata aaaattaccc
    13321 ctttcgtcta ccacaatcaa gaaggagaag gaccactctt tccctcttct taattctatt
    13381 atatcgaact ctaggttatt tgcaaattaa atcgctatca aatttccaca tctaattcat
    13441 ctcgccaaag aaatggttcg gatgattttc ggctgtgcga cctgcaaaca atctgctgcg
    13501 tggaaaagag tctgtcctgt tgtatagaca tcatcaataa tcaatatttt tttattttgt
    13561 atcttagaaa aggtgactgc aagttcaaaa ggttgaggca tctccaatct ttcctttcgc
    13621 gtgttttttg cttgagccag cagatgcttc tctcttttga gtagcatctg aaaaggaatt
    13681 cttgctgctt tcaacatctc gcttacttga ttgaatcctc tttctgttcg ccttttttca
    13741 gataaaggta ttggacaata gatgtatgtc gggtattctt tttttatctg cgtccattta
    13801 tatgcaaagg tgcctgctag tcggatatct cccataaact tgtattgatt gagccaggcc
    13861 ttcatatttt catcgtacac aaaaaaggca cggtgctgaa aatcataggt cggatatttc
    13921 ttcttccatt cttgacagtc actgcagtaa acgggaatag actttacttt ctttttcgta
    13981 ctctctagat tgaaatggat tggtttttga cagccagaac agcctcttgt ttccaataaa
    14041 tgaaattgtg cgcgacaaac tgaacaagtc tgaggtaatc tgtatttcga aacaccaata
    14101 atctcgctga atgttaaatt ttggaagata tctttactgc agcaactgca cctcacagca
    14161 tcttcttccc ttcttcattc atttctttga tttcctgaca agcttttcga atagctgcag
    14221 ttttttcttc atagaaaaag tatacttgac tgttcgtata aggcccttta cgatcggctc
    14281 ttcctgcgat ttgtaccaaa gaagatttag taaaaaccgg atgatccgct cctaaaacga
    14341 taacggatat tctttcaaat gtgactccac gttcaaggat catcgtggtt aacagtatat
    14401 catattttgc ttctcgcatt ttctggactt tttccgcccg ctgattgtct cttgaagaaa
    14461 catctgtgat ctcatatctc ggaagagcct cttgaagtgt ggctttcaaa cgatgcatca
    14521 tcgtgatact tggacaaaat aaaaggatat ggttttcttt aattaattga tgaatgatcc
    14581 gcaccaaaga ttttatcttc ttcttgttct tgcatttttg tcgccaatta ttcatgaaaa
    14641 ccaagacggg ttcaggaagc aaacgccgat gaaagcgtaa agcaagtttg tggatgtcaa
    14701 acgtctgctt gatttccttt agtaactttt tatcaggtgt ggcacttaag tagatgaatc
    14761 gtccgtctgg tttaatcgct gtttgcacag catgagctaa tcctgcatcc ccgttataag
    14821 gaaacgcatc gatctcatcg atcactatta aatcaaagga ccgataaaaa cggtatagct
    14881 gatgaatcgt acagataaca aaagaagtaa aggtataagc cgttgctgta ttcccatgga
    14941 gcaacgtgat tttttcttct ggaaaaacat aacgatagcg taaaaacaat tcattacaaa
    15001 catcgactcg cggtgtacaa atcgctactc ttcctccagc ttttaaagtt tccactaaaa
    15061 taggataaag catctctgtt ttccctgcac ctgttacagc ccaaataaga tgatgtcttt
    15121 tttctgaatg gcaaagtatt ctctttgaga tttccgtttg tttttcagta agctgtcctg
    15181 tccaaaaaaa gacgacttct cgaggttttg cttcaggctg atcaaacaaa tataaatctt
    15241 gttgagtgtc gcatcgccct aaatatagac aatgtgtaca aaaataaccg taatctgcaa
    15301 gctgtccatc aatgacggac gtcctcattc cacagcgatg acaaagaatc cagttgccca
    15361 aacgttccac tgctgcgatc ttctcataat ttggtaaatt tacctcctca cagtctattg
    15421 cttcaatatc tctcgtcaga agccttctac cccaaaaatg attcatattc ttcgccctaa
    15481 aaaaatatac gaaaaaacaa gcgatctatt ttttggatcg cttgttcgat agttatactg
    15541 ttctatttat tcacctatga gcgtgattgc ttgatctaac actttttcgc cattcatcat
    15601 accataatca gccatattga tgacatctaa aggaatgcct tttggttcca gacgtttctc
    15661 aaaatcagct ttcataaagc gaacttgtgg gcctaataac aacacatcca cgtctttatt
    15721 tgcaaggttg ttatctgcgt cagaagcgga cacagcgaaa atatccgagt cgattccttt
    15781 tgcttcagca gctttttgca tttttgttac tagtaaactt gtgctcatac cagccgaaca
    15841 tacaagcatg attgtttttt tagccatact tttctctcct ttttaaaaca aactatgcaa
    15901 acgttttctt cgcgtctgtt actctattat ataaaaagaa agaaatttgt caacgttttc
    15961 taaacgcggt ttatttaatc aaatagccaa ctagatttca ctaatttatt gaacacgaca
    16021 tattctttgt ccaaattgat gacaactact tcatagcctg catttatcca gccataggca
    16081 cctttttcag ggtgtttata atcatttgcc caccaatctg tactttccct tgctgttttg
    16141 ggaagtccgt tactgggaaa cagcagttcg tcaaattggg taaaggtcaa cgttacttga
    16201 gagccgccat tgttcttcaa ataatgcgta attgcatcgt actttttcat tcgttcctgc
    16261 atccccagat ccacctgcct ttgtctttat tatcgcatac tacagcaatt gcgcaaccgt
    16321 tacagtgttg aaacaaccta gaactttatt tatactgaat aaagaaaatg aaaaaaagag
    16381 gtgacaagat gatcgagcgc tatcgtacaa taaaagaaaa tggacaaagt gagattgaga
    16441 tcaaaaaatc acgctttatc tgttctctta gacgaataga atcagaggac caagcaaaaa
    16501 gtttcattca agaaataaaa aaagaacatt ggaaagcgaa ccataactgc agcgccttca
    16561 tcattggaga aaaaaatgaa atccagcgaa gcagcgatga tggagaacca agtggaacgg
    16621 cgggcgtacc gatgttggaa gtcctcaaaa aaaatcaact gatcaatgta gctgcagtag
    16681 tcactcgata ttttggagga acgaaactcg gcacaggcgg attaatacga gcatacactc
    16741 atgcggtctc tcaagcattg tctgacattg gaatcgtaga aggtaccttg caacaggaga
    16801 tctctgtgat gatcacgtat ccgcaactag gaaaattaca gaattatctt gaactccaac
    16861 atttttttat aaaagaaatc caatatactg atcaagttag cgtcatctgt gctgtagatg
    16921 aaaaaagtgc tgaccacttt aaagaatcca tcattgagct gctgaacgga caagtccaat
    16981 ttgaagaggg ttctgtttct tatcacgagc aactgataac tgatcatata taaaaacagg
    17041 tgattctgat tctcttttat ttctaataag gaatcaggga tcacctgttt tttatttttc
    17101 caaataaaac tcaaaacgat ttcctacgta ttgcgtacgc acatactcaa acggcgtacc
    17161 gtcttcaaaa taagaaatct ggcgcaaacg aagaactgca tctcctcgtt ttacctctaa
    17221 atagtcagca attttctcag aagccaacac agcagtaatc gtttgattcg ctggaccgat
    17281 ctttttcccg cctttttctt ctagcgtgcg atacaacgat tcagtaatct ctgctttgct
    17341 gtagccatcg atcagctttt gtggaacact ggcaacttca aaacaaatag ggacatcatc
    17401 tgcgaaacga atacgttcca ttcgcaagat cgtgtcatct ttactcaagt ttagtttttc
    17461 catttcactt gagcttggcg aagtcaagaa ataagagatt gtccgactag acggtactcg
    17521 attttgtgat tccataattt ctgtaaaact tgttgcacca gacatttttt cttgtacttt
    17581 ttctcgcgca acatacgtac ccgaaccgat ctttctttct aagattcctt catctgccaa
    17641 gttttgaatt gcttgtctca acgtcatacg gctgacattg aatttcactg ctaattctct
    17701 ttctgaagga agacggtctc cgactttcca tataccctgt tcgatttcat tttttaattg
    17761 gtcatgaatc tgtatatata caggtaattg attcgacata acgtacaccc gtcctttcgt
    17821 ttcttctatt ataactggta tatacctgat tcacaactga aaaagataaa ggtttctatc
    17881 catcaacaga ggatttagac acacatcgtt ctccctactc ctattgtcag aaacaatttt
    17941 ttagatatgt tcaacctaga aaataacaaa agaccctaaa atcgatgaaa atgccgactt
    18001 atggatcttt tgttattatt agcggttttt agccatccat ttttttgtat gattcgttgg
    18061 gcgttttgat tttacctgga ccgcttttcc gccttttttc ggaatattca cttcaatatt
    18121 gtatttttct gatttgacca aatcatataa cttttcagct gcttcgtcta ctttttcttt
    18181 ccatttcatg tggatccctc caattcttgt agttaactta actctatctt aattcagcag
    18241 aatcttcaag gaacaaggta gcaaagcaaa aaaaagagct ctcgaactaa c
//
LOCUS       DACEMO010000035        18155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEMO010000035 DACEMO010000000
VERSION     DACEMO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.035"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(291..1874)
                     /locus_tag="IW728_000930"
     CDS             complement(291..1874)
                     /locus_tag="IW728_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1024306.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2452..2595)
                     /locus_tag="IW728_000931"
     CDS             complement(2452..2595)
                     /locus_tag="IW728_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1024307.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2818..3297)
                     /locus_tag="IW728_000932"
     CDS             complement(2818..3297)
                     /locus_tag="IW728_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1024308.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3370..3654)
                     /locus_tag="IW728_000933"
     CDS             complement(3370..3654)
                     /locus_tag="IW728_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024309.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3732..3926)
                     /gene="ssrS"
                     /locus_tag="IW728_000934"
     ncRNA           complement(3732..3926)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW728_000934"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3998..5284)
                     /locus_tag="IW728_000935"
     CDS             complement(3998..5284)
                     /locus_tag="IW728_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1024310.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5422..6093
                     /locus_tag="IW728_000936"
     CDS             5422..6093
                     /locus_tag="IW728_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1024311.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6059..6547
                     /locus_tag="IW728_000937"
     CDS             6059..6547
                     /locus_tag="IW728_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1024312.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6560..7507
                     /gene="prmA"
                     /locus_tag="IW728_000938"
     CDS             6560..7507
                     /gene="prmA"
                     /locus_tag="IW728_000938"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1024313.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7508..8272
                     /locus_tag="IW728_000939"
     CDS             7508..8272
                     /locus_tag="IW728_000939"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1024314.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8505..10718
                     /locus_tag="IW728_000940"
     CDS             8505..10718
                     /locus_tag="IW728_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1024315.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10729..11175
                     /locus_tag="IW728_000941"
     CDS             10729..11175
                     /locus_tag="IW728_000941"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1024316.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11346..12605
                     /locus_tag="IW728_000942"
     CDS             11346..12605
                     /locus_tag="IW728_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1024317.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12694..13641)
                     /locus_tag="IW728_000943"
     CDS             complement(12694..13641)
                     /locus_tag="IW728_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1024318.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            14035..15327
                     /locus_tag="IW728_000944"
     CDS             14035..15327
                     /locus_tag="IW728_000944"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1024319.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15342..17111
                     /gene="aspS"
                     /locus_tag="IW728_000945"
     CDS             15342..17111
                     /gene="aspS"
                     /locus_tag="IW728_000945"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1024320.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            17220..18095
                     /locus_tag="IW728_000946"
     CDS             17220..18095
                     /locus_tag="IW728_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1024321.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
BASE COUNT         5737 a         3377 c         3876 g         5165 t
ORIGIN      
        1 aaaagtggtc aaaagggaaa agactggcat tttatttatt gattttcagg aaaatcccaa
       61 tattttattt tcattcatct ttataaggga cattacatat gtaaaaagaa aaaacatttt
      121 tagatcagcc tcgtgggctg tcgactttat tcttcgcttt atgtttagct agaaaaccga
      181 tccgaaacat tcattgatcg aataataaaa acttgatttt ccacctgtac tttgcctgaa
      241 tcgtgaaagc acaggtatga aaatcaagtt ttttgttgcg aaaaaacgtc ctagtcaatc
      301 agttggccga gcggctggcg gctgatctgc tcctgatcaa taacgagtgc ttcttggaag
      361 tgttgctgca aaaagtagcg gtcatggctg actgctagga gcgttccttg atagtcttgc
      421 agcatttctt caatttcttc tcgagcatag ctgtcgaggt gattggttgg ttcatccaaa
      481 atcaggacgt tgattttttc taaaaagagt ttcagcaacg ccaaacggat tttttcacca
      541 ccggataaat ctttgatccg tttacccaca tcgctttgat aaaagccact tttggctaat
      601 tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg cgtaagccag caaacggcta
      661 tcgggatcgg caaactgcag ctgctgcggc aaataaccga tttgcaagct actacctaat
      721 ttgatggtgc caccgtaagg cttttcctca ccgagtaata atttgagtaa ggttgatttt
      781 cctgcaccat tgtcgccgat gatggctaaa tgatctccac gatagactgc aaaactgctg
      841 tctttgaata gtacttgatc agcgtactgc tggtgcaagt tttgaatgat gaaaacttct
      901 ttgccagatt ttccaccatt agacaagctt tgcagccgat ttttcggggg aggtggcggt
      961 ttgacgagtg tcagtttcgc caggcggcgt tccagttctt tggctttttt gaagaaggac
     1021 tcattgtctc cttcatgtgc ccactgacga aaacgacgaa tcatgacttt caaccgctgg
     1081 acttcttttt gctgcagttc gtaatttttt gtcagctcag ccaagcgagc ttctttcaag
     1141 actcgaaaac ggctgtagtt tccgggatac tcaatcaatg caccttcttc cagttcccat
     1201 gtttttgtgg tgacttgatc gagaaattgc cgatcatgag aaacgaccag aaccgatttt
     1261 tttgtattct gtaaatagtt ttccaaccag cgaatactgt taagatctaa atgattggtc
     1321 ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa ttttggctaa agcgacttta
     1381 actcgttcgc ctccgctaag ttctgttaac cgtgcttcct gacggtgtgg aatccctaag
     1441 ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac cgcctagttc ttcaaagcga
     1501 tgctgcagct caccgtaacg ggccagacac ttctctaagt ttgtatctgg ttctgtcatc
     1561 aatgcttcgt agcgactcat ttgctgtttg aggtcctgta tctcagcggc agaatggagc
     1621 aagtagtggt tgacggtcgc ttcggaaact gtaagttctt gttcgaccgt cccgatcttt
     1681 aagccttttt tgcgactgat aacaccgctg tcgacacctt ctctgccggt caagatttgt
     1741 aaaaaggttg atttgccagt tccgttgata ccgactaagg caattttttc accagcttca
     1801 atttgacagg ataaatcctc aaataatgga ttcccgccga ttgttttttt aatagaaatt
     1861 gcttgtatga tcatgatctc tctccttgag accactcaaa aaggccatgg ttatccatgg
     1921 cctgtgttat ttaaactcag attatcatga gaaaaatacc ttgagatggc cattgatttt
     1981 acgtgaattc aactgttttg gacaaacttg tcccgtcaac agcaactgta aaaccaattg
     2041 catggctaaa tgcgatatgg tatcgttggt aattttcatt acttgttatt ttagtcatgc
     2101 gcatcatagt attttccctc cttcgtgtag ttgtcttttc cagtgtacct gaaagtgtca
     2161 gaaagttcaa ctagcgctga catttactta atatttatcg atgtgtaatt ctgtcagaga
     2221 gcttttttta tctgactgag ggtaatgtct cgtggaccac atcattgtat tctgagctaa
     2281 accagcgttc aggcgaaatc atatggtgtt tgataaaaaa ctttgtctac atgacttcct
     2341 tttgccattt gcttaatacc gttgcattaa acagctcttc tctttggaaa tctcaccaac
     2401 atcgcgttca ggagtatcgt tgtggttgca atggcaagcg gtagcctttc atcaattctt
     2461 gaatgattta ccgaatgtag cacttgcctt ctcgcggtac ccgaacccga aataaaattt
     2521 tagtttggcg ataaaatcgg tatccgccat cgatacttca tattttttcg tatcagattc
     2581 atcggaaact tccacattga cgaaccaata agctttggca tgcttaggtc gttttaaacc
     2641 acaccagggc gactaatcgc cctggtgttc actttttttt attttacttg aactcattga
     2701 ctgttttccc tttattttaa acattataaa gaccgatgag tgcttgccgc gtcttttgtt
     2761 tttatcacgg ttttttgtgc aaaaaaacct gccgatagtt ggcaggtcgt ctacgtgtta
     2821 agctttggtg tggtctttag ctggcaattg gttttctaaa tcagttcgga caacaagcac
     2881 atcgcaagct gcgtttctaa taacatattc agaaacagaa ccaataaata aacgttctac
     2941 agcattcaat cctgtggctc ctaacataat caaatctact tgattatctt ctgggatttc
     3001 tctggcaata atttgttttg gtgagccata ttctacgaca gaggttacat tattcaagcc
     3061 agctttcttc gcattgcttt cataatctgc caatgtttgc tttgccattt cagtagcttg
     3121 ttctgcaagc attccatcaa aggatgaaac ggtttggaaa gaacgtgtgt caatgacatg
     3181 tgctaataga agttctccgt tatttcggat agctacattg accgcctttt taaaagctaa
     3241 ttcagcttca tcagaaccat ctacggcaac catgattttt ttgtattgtt gtaacataag
     3301 cattcactcc tcaagtcaat cgtattgtac tcttttattg tagaacactt tagggaaaaa
     3361 gagaattatt tactgctttg gttcagcaat tttttttgca aatcggatac tgaaggtggc
     3421 agaaacaata attacgatca gtaaaaagac caaggcaata aagcgttgat tgaaaaaagc
     3481 tgttaaaata cccattacgc ctaaaacagc atatgcagct ccgtaaaatt gtaggaatcc
     3541 ttgattttta tcatttttgt tgatcaatac aaaaaagata tcctgtttat taaataaata
     3601 atagctaacg aataataata ctaaagtggc gataatcatt aaaatcctaa tcatgaacaa
     3661 atcctccaaa ataagcaaca aatagaaaaa gcaatttgcc aagagcaaat tgctttattc
     3721 tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc tgtagtattg atcccgcgaa
     3781 ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc aaatgataag gcatgttacc
     3841 agcagtgaca caagacgggt cccaagatat caataaaaat gttcggtcaa cacacaaaga
     3901 ggacaattcg aacatcacag atttcccacc tctatagtag catatgaatt taaaaagagc
     3961 aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta gttttttgga ggatgctttt
     4021 tccactcttt tatacggtaa tactgttgac ccagagcctg ttcatatttc cctgtatctt
     4081 ttggctgata atactgagca ttcttcagtt tatctggtaa atattgctga ttgacccaag
     4141 cctgatcaaa atgatgagga tactgataac ccacgccacg gttgagtgat ttcgcacctt
     4201 tgtaatggct atctctcaaa tgatcaggaa catctccagc tttgccctct cgaatatcag
     4261 caagtgctgc atctaatgcc atgtaggcag aattggattt tggcgacaag caaagatcga
     4321 cgacgacgtc tgctagtggg atacgtgcct caggcaatcc caatttttca gcagctaata
     4381 cagcgttcac tgtacgagcc gcagcagcag gattacctaa acctatatct tcataaccga
     4441 taaccatcag acgccggcaa atacttgcca gatcacctgc ctcaactaaa cgggcgagat
     4501 aatgaagtgc agcatccaca tcacttccac gaatcgattt ttgaaaagcc gaaataacgt
     4561 cataatgagc atctccattt ttgtcatggg taagagcttt tctttggaca cattcttcaa
     4621 tgatcgacag tgtaagatga atccttcctt ctttatctga aggagtcgat agtgtagcca
     4681 gttccagccc attcaatgca ctccgcaaat cgccgttcgt tgcacgcgaa aggtgaagaa
     4741 gagcatcttc gtctaactgg attgcttgtt gtccaaggcc gcgttcttta tcccttaaag
     4801 catgttcgac tgccaactgg atgtcttgtt ctgttaatgg tttgacttca aagatctgtg
     4861 tacggctgcg gatcgctgga ttgatggtaa tataaggatt ctcagtagtc gctccgatca
     4921 agatgattcg gccactttct aaatgaggaa gcagaaaatc ctgttttgtt ttatctaatc
     4981 ggtgtacttc atccaataag aggatcactg taccactcat ttttgcttct tccgctacaa
     5041 cttgtagatc cttttttgaa tctgtagcgg cgtttagcat gcgaaatgca taattagtag
     5101 agccggcgat cgcacttgcg atactcgttt ttccagtacc aggaggtcca tatagaatca
     5161 tggaagagag catgcgagca tcgaccatgc ggcgtatgat ttttccttcc ccgactagat
     5221 gctgctgacc aacgacttca tcgatcgttc gaggtctcat gcgataagcc aatggctgtt
     5281 gcataggatt ttcctccttc ttgtttttac gtcccttaat tatatcatat ttagcaagat
     5341 gcctagaaag aacagccttc tgttgttttg ttttcatagc ttatccaact aatattgtgt
     5401 atgataaagt aggtgagaaa aatgacggat aaacaaaaga tagatgaatt tttttggaat
     5461 aaaacgacgc cagaagtggc acgggattta ttggggatgt atttggagca tgacacacca
     5521 gaaggaagat tagctggata tattgtagac gcggaagcat acctaggtcc agaagacgag
     5581 gccgcccata gctatggttt aagaagaaca ccgcgtgtgc gggcaatgta tgaaaaacca
     5641 ggtacgatct acctttacac gatgcatact catcggattt tgaacatcat tactcagcca
     5701 gaagggattc ctcaaggagt aatgatacgt gcaatcgaac ccgccgcaat gattgatcaa
     5761 atgagtaaaa atcgtggcgg aaaaactggt ccagatatca gcaacggtcc gggaaaatta
     5821 gtggaagcat tagcaattcc tcaagaactt tatggtcagt ctatagcaga tagttcgtta
     5881 cgcttagttt ttgagaaaaa aaagacacca aaaaagatca atgctttgcc tagaatcggt
     5941 atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt ttgttgtgtc gggaaatcct
     6001 tatatctctt tacaaagaaa aaaccaaatc gagaaagatt ggggttggag gaaagaaaat
     6061 gaaaaagaag gatctattaa catatttaga tgaaataatt gaaaaacaag tgacagacta
     6121 tgatgtagct ttagattggg atacaaaaaa tcatacgatc gagatcgttg ttcgcttgtt
     6181 tgcagaaaat aaagcacaat ttgagattga tgatgcagaa gggatcgttt cgcaagaacc
     6241 aattatcgaa tttgaagacg gcgtacttct ttttaatcct cagaaatctg tttttgatga
     6301 acgcaattat ctagcagtaa tcccttatga aggaaaaaaa gggttggcaa aatcagtagc
     6361 tgattcttta gtggaatatc taaatgaagt attagctcaa gggcaaagcg atttgcttga
     6421 ttttttggat gaagacgatg acgaagctgt attcgagctt cactgggata atcagcaact
     6481 agcagaatta gtagaagtaa aacagcaaaa tgaagcagat acttatttac cgtacccaag
     6541 ttattaagga gtgacagata tgaaatggaa tgaagtaaaa gtagaaactg aaagtgaagc
     6601 agtcgaagca gttgctaata ttttgatgga ggcaggggcg agtggtgtcg cgattgaaga
     6661 ttctttagat gttgaacatt ttcagtcgga tcaatatggt gagattttag ataaaaatga
     6721 ttttacatcg ctgaaagaag gcgcactagt catggcttat tttccggaaa cgactttctt
     6781 accggaaatc ttgcctttta tcaaagatcg tatcacacaa ttacctgagt ttggtctagc
     6841 tattggaaaa aataaagtaa cagttagtga agtagaggaa agcaactggg caacagcctg
     6901 gaagaaatac tatcatccgg tgcgtattac gcgaatgctg acggttgcgc caagctggga
     6961 aaactaccag acagaagatc ctctagaaaa aattatcaca ctagatcctg gtatggcatt
     7021 tggcacaggc actcatccca cgacttcctt gacattgaca gcactagaaa cagtcttgcg
     7081 tggtggtgaa acattgctag atgtgggaac tgggtcgggc gtgctaagta ttgcagcaaa
     7141 atatcttggt gcaaaagatg tctatgcctt tgatttagac gaagtagccg ttcgttcggc
     7201 gaaagaaaat atggatatga atgaaatagc caaagatgtc catgtatcag ctaacgatct
     7261 tttgacagga atcgagattg aaagtgatgt aatcgttgcg aacattttag cagatatcat
     7321 cttattgatg atcccagatg cttggcgttt actgaagcaa acaggcacgc tgatcgtttc
     7381 aggaatcatt gaagagaaga agcaaatggt tctggatgcc atgttagaac aagggtttat
     7441 cgtggatcaa attttccagc aaaaagactg gtatgcaatt attttgaaaa aaccagaggt
     7501 ggaatagatg cagcggtatt ttttaaaaga atcatatgaa tcaaaagact attttgtttt
     7561 agaaggggaa aactatcacc acattgtacg ggttatgagg atggaacccg aggatgaagt
     7621 ttttttggca ttcaatgatc aaatagccat tgttgcagaa atcacggaga ttactgaaga
     7681 ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa aaggaactgc caagttcgat
     7741 taccatagcg agtggctatc ctaaagggga aaagctggat tggatcgttc agaaatccac
     7801 tgaattagga gcgcaagctt ttcttggttt cccggcatca gcctctgtcg ttaaatggga
     7861 tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag attgctcaag aggcagcgga
     7921 acaatcccat cggcagatcg tgccatcagt agagttattg gaaacgaaac aagatttatt
     7981 tgagcggcta gatgattatg acgtggtctt ggttgcttat gaggaatcag caaagcaagg
     8041 cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa ccagatagtc gaatcttagc
     8101 tgtctttgga ccagaaggtg gattatcccg cgatgaagtc gattattttg tagaacacaa
     8161 agggatactt tgtggattag gaccaagaat ccttcgtaca gaaacagcac ctctttattt
     8221 attaagtgct gctagctatc aatgggaatt aatgaatcct tctttcagct aacgttcttc
     8281 attaataaaa gagtatcgac agaaagaatt gaaaaaatag tcaacaattt gtataataga
     8341 aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa aagcttttaa aaagcgaaca
     8401 tttccgtaaa aaaacgtata actgcacgga ttttgggaca attatcaaac agatatttgt
     8461 cccgattccc gttttaccga aaaaagaaag ggggatcgat tcctgtgaca aaagaagaaa
     8521 ttttaacggg acccggtgtc ataaagctcg ttagtcaata catgggtccc gaacatgtag
     8581 catttgttga gaaagcatgc gaatacgcga ctgctgcaca tgacggacag tttagaaaat
     8641 caggcgaacc ctacattatt catcctatcc aagtcgcagg tatattagca gatttaaaaa
     8701 tggatcccca tacagtggct acaggcttct tacatgatgt tgttgaagat acagaaatca
     8761 ctttagaaga tctgagagaa gaatttggcg atgacgttgc tatgttagtg gacggcgtaa
     8821 ccaaattagg gaaaataaaa tataaatccc acgaagagca gctagcagaa aaccaccgaa
     8881 agatgctgct agcaatggct caagatttac gagttatcat ggtcaaatta gctgatagac
     8941 tacataacat gcgtacgttg aagcacctgc gagaagataa gcaaagaagg atcgctcagg
     9001 aaactttaga aatctatgcg cctcttgcac atcgtctagg gatcagccgg atcaaatggg
     9061 aattagaaga tacagcactt cgttatctaa atccaaaaca gtattaccgt atcgtccact
     9121 tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag tggtactgtt gaagatattc
     9181 gaatagcgac agaagagctg gggatttttg cagaaatcta tggacggcca aaacacattt
     9241 attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt caacgaaatt tatgacttgc
     9301 tagctattcg agtgatcgta gattcaatca aggattgtta tgctgtatta ggagcaatcc
     9361 acacaaaatg gaaaccaatg cccggcagat ttaaggatta tattgccatg ccaaaagcta
     9421 atatgtacca atctttgcat actaccgtga tcggtccggc aggaaatccg gtagaaattc
     9481 aaatccgaac acaggaaatg catgaaatcg ctgaattcgg ggttgctgca cactgggcct
     9541 ataaagaagg aaaaaatgaa aaagtagaac cagatggtat gacgaaacaa ttaagctggt
     9601 tccatgagat actcgaactt caagacgaaa gctatgatgc ttctgaattt atggaaggcg
     9661 taaaaggaga tatctttagt gataaagtct acgtcttcac accaaaagga gacgttactg
     9721 agttaccaaa aggatccgga ccattggact ttgcatacag tatccataca gatatcggta
     9781 acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca gcttgattac aaattgaaaa
     9841 acggagatat cattgagatc atgacttctc caaattcatt tggcccaagt cgcgactggt
     9901 tgaaattagt tgctactagc aaagcaagaa ataagatcaa acgtttcttc aaagcccaag
     9961 atcgagaaga aaatgtgatc aaaggccacg aatccgtggt caaatgtatt acagatctag
    10021 gatttacgcc taaagatatt ttgacgaaga acaaactgca agaagcactc gatcgtttta
    10081 attatcaaac agaagatgat ctctatgcag ctgtagggta tggagaagtt agccccttga
    10141 cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca aaaaatcgag cagcaaaagc
    10201 aagaagcaga agaaatcatg aatcagccga aaaaagaacc tgacaaaatg aaagtacgtc
    10261 atgaaggtgg cgttgtcatt caaggggtag aaaacttact aattcgtatc agtcgctgct
    10321 gtaatccgat tcctggtgat gatatcgttg gttatatcac taaaggcaga gggatatcca
    10381 ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc aaatgtagca gaacgtttga
    10441 ttgaagtcga atgggaagat acatcgaata cacgaaaaga gtatgatgca gatttggaaa
    10501 tttacggcta taatcgttca ggcttattga atgatgtact tcaaacagtc aatgcgctaa
    10561 cgaaaaatct caacagcgtt gaagcacgga cgaataaaga taaaatggcg acgatccatt
    10621 tgacggttgg tatccagaat ttatcccacc taaagagtat cgtggataaa atcaaagcag
    10681 tacctgatgt ctacagtgta cgccggacga atggatagga ggacagatat gagagcagtt
    10741 gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc aagaaattgg aaaaattggc
    10801 tacggcctac ttgttttatt aggcattcat gaaaccgata cgcaaaaaga tgtggactat
    10861 ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg aacaaggaaa aatgaatatt
    10921 agtgtagaag atgtgggagg acaaatttta agtatctctc aattcacact gtttgcggat
    10981 acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac gaccagaaac agctattcct
    11041 ttatacgaat cattcaatga aggaatcaga agtcgcggca tcacagtcgc taccggacaa
    11101 ttcggagcag atatgtcggt ttctctcgtc aatgatggac ctgtaacgat cattattgat
    11161 tctaaaaaca aataaagtaa tctggttttc actagtaaaa actatgaaac acgtcgctta
    11221 agttagactg ataaactcta acttaagtgg cttttttgtc tgcaaaaagc gcttcttttg
    11281 tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt tattttttat aggaggttgc
    11341 tgaagatgaa agaagtacca gtagtagata tcaaaatcac agatttaaaa aaattgtatc
    11401 aaaaaggata caacatgttg gaggagttac gccatgaagc ggacgctccc gtcgtcaaag
    11461 caaagatttt caataaagaa gctatcacga tctacggatc atcagcagca aaagtatttt
    11521 atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa attagtattg aagacacttt
    11581 ttggtcaagg tggagtacag actttggatg gagctgctca tcaccatcgg aaaaatattt
    11641 ttatggatct aatgacaccg gaaagaatgg aagattatca tcgtatctta gataagaatc
    11701 tgacacaagc acttgaagca cagcatggtc aatttgaact atttgattta agtaagatgg
    11761 ttttctttac atctatttgc gagtgggcag gtatcaattt atcagcgatt tctaaagatg
    11821 aggtcgaaaa attagcagaa tatcaaatct ctatgatcag cggaacgttt acttctccga
    11881 ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga aaaatgggcg caaggcttga
    11941 ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa tgtggcatta tatgcttttg
    12001 ccaatgccac agatttagat ggtcagcttt tacctctgga agtggcagct gttgaattat
    12061 tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc ggcattgatg ggacatgcac
    12121 ttttcagtag accagatttg tatcagcaat taaaaaatga cttttcgacg ttacaagatc
    12181 cgtttattca agaaatgaga cgctattatc ctttcttccc aatgctacct gcgatttcgc
    12241 tgaaagaagt agaagtagat ggttatcgta taccagaagg cagttgggtt attttagatt
    12301 tatatggaac agaccatgat gaacgaacag ttgaagcgcc agattccttt atgatcaaac
    12361 ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga atatgagatg attgcacaag
    12421 gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga atggatcact cttcatagtc
    12481 tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt ctctgtacct gaacaagatt
    12541 ggacgattcc attcaatcag tttccgactt atccgaatag ccgagcgcta ctttacaaga
    12601 attagattag cataaactta tcgaaaaaaa ggaagatcaa acagtgctga tcttcctttt
    12661 ttcatattac tataaattga taaggtcaat tgatcaaaat ccacgaaatg cttcatcaat
    12721 ggtttgataa tcttcattgg ataaatgcaa atctaaagct tggacattgt cagatacttg
    12781 ctctggacgc ttagctccag gtataactac agacactcgt ggattcttca tgtaccaagc
    12841 taaaacaagt tgcgcaggag tggcttggta tcttttagca atggggcgaa gtacatcgac
    12901 agcggttaaa atttctctga aacgttctcc ttggaaatct ggatttcttt ttcgaggatc
    12961 tccttcacca aattgttttt cctcaccaag ttcatatttt cctgtcagca aaccggaagc
    13021 aagaggaaaa tagggaacaa aactgatttt gtttttttca agataaggga ataattcttt
    13081 ttcagcctgc cgatggatca aactgtattt atcttctact acatcgacat atccatctgc
    13141 attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca ccgactgctc ggatttttcc
    13201 tgcttctttt aattcgtgca atgtagcaac tgattcattt tttggtgtgc tttcatccgg
    13261 aaagtggata taaaaaatgt cgatgtaatc agtttgcaga cgttttagtg catcttccac
    13321 cgattgtttg agaaactcag gagaattatc aaatgttcgc cctttatttg gtacatgagc
    13381 agcttttgta gcgatgacga cacgtgagcg atcatattct ttttccttca gtacctcgcc
    13441 aatcagttct tcggaacggc cgtttccgta agcataagcg gtatcgatca aattgatccc
    13501 gctattcaaa gctgtacgaa ctacttgttt tcctgtctca tcttccaaac cgtcaaaaag
    13561 attatgtcct ccaacggcat ttgctcctaa tccaagctgt tctgcgtaga cttgtgaatg
    13621 tccgattctt atttctgcca tagctgattc ctcctttttg tctagtctaa aacaaaatac
    13681 caaaaagctg aactaaaacg tttttgtgag gggaaatagt cagatatctc ttgactttga
    13741 gactggtttt tagtaggtta taagtaatat cgaatgtcaa tgaaagagcg agtaagtttt
    13801 tgaaacttta caagagagga tcatcgaggc tgaaaatgat ctaaagtttc attagcctaa
    13861 agacactctg gagatggacg agtgaaaagc cgtcccgcgt acgagcgtta ttcgtaagag
    13921 gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga atttcattca cccttgttca
    13981 tgatgaacaa gggtgatttt tttatttaca tagctaagaa agggagagaa aactatgagt
    14041 ttccaaagac caaaaggaac aaatgatatt ttgccaggcg aatcagaaaa atggcagttt
    14101 gtcgaagaaa cagcacgttt attattcaga gattatcagt ataatgaaat gcgtacaccg
    14161 atttttgaac actatgaagt gatatcccga agtgtagggg atacaacgga tatcgtttca
    14221 aaagagatgt atgattttta tgacaaaggg gaacgccatg tgacgcttcg accagaaggt
    14281 acagcaccaa tcgttcgttc ttttgttgag cataaattgt tcggcccaga gtatgcgaaa
    14341 ccgttcaaaa ctttttatat gggaccaatg tttcgctacg aacgccctca aaaaggacgg
    14401 ttacgtcaat tccatcaaat cggagtagaa gcatttggat cagaaaaccc tgcaactgat
    14461 gtggagagca tggtgatggc acttgatttc ttcaaacagt tggggatcac gcaaatccgg
    14521 ctagtcatca attcattagg tgataaagaa acgcgtcaag cctatcgtca agcattgatc
    14581 gactatcttt taccttttga atcagagctg agtgaagact ctaaacgccg ccttcatgaa
    14641 aatccattac gcgtattgga cagtaaagac aaacgagatc aaaaatttgt ggcagaagct
    14701 ccatctatct tagattatct aagcgagcct gctcaaaaac attttgatac cgttgtttcc
    14761 atgctggatg cattggctgt tccttatgaa atcgacagta atatggttcg cggactagat
    14821 tattacacac atacgatttt tgaaatcatg agtgacgcac caaaaatggg ggcacaagca
    14881 acgatttgcg caggcggacg ttatgacaat ctagtggaag aactaggtgg tccttcaaca
    14941 cctggcttcg gatttgcaat gggtatcgag cgattactct tgactatgga ggcagaggaa
    15001 gtagttatcc ctgcatttaa tgagttggat gcttacgtgg tcgcactcgg agacgaaaca
    15061 aatatcgaag cattgaaagt ggtccaagcg attcgaaact ttggtttttc agctgaccgc
    15121 gacttcatga accgtaaagc aaaagcgcaa tttaagacag cagataaagc gaatgccaaa
    15181 ttagtattgg tgatcggtga agatgagctt gctagtcaaa cgattaatgt caaagcaatg
    15241 gcaagccgaa aagaaaaaag ttttccatta gaagagatat atgaacattt tgatgaagtc
    15301 tatgatgaaa tgactttatt cgagtaggag gaaaaaaata aatggcacaa agaacgatct
    15361 attgcggatt agtttccgaa caacaattag aacaaacagt tacattaaaa ggatgggtac
    15421 aaaaaagacg agaccttggg ggagtaatct ttatcgacct acgtgatcgt gaaggaatcg
    15481 ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg ggaaatcgcg gataaatgtc
    15541 gcagtgaata cgtgatcgaa gtgactgggg aagttagaag acgcggagaa aatgcagtaa
    15601 atcctaaaat ggccactggt gaattcgaag tgattgcaag tgaaatcgtg attttgaatc
    15661 aagcaaaaac cccgccattt caaattgaag atgatcaagc aatcagtgac gaaatacgtt
    15721 tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac taaaaacatc aaattaagaa
    15781 atgcgacaac aaaagcgatt cgtcattatt tagatgataa tgactttctc gatatcgaaa
    15841 caccttacct agggaaatct acaccagaag gcgcaagaga ctacctagtt ccttcacgtg
    15901 tccatgctgg acatttctat gcgttgccgc aatctcctca attgtttaaa caattgctga
    15961 tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg tttccgcgat gaagatttac
    16021 gtggagaccg tcagcctgaa tttacgcaag tagatattga aacaacgttc ttaacagcag
    16081 aagaaatcca aacttacaca gagggattga ttgcgaaggt aatgaaagaa gttcgcggta
    16141 tcgaagtcac attgccattt cctcgtatga cctatgatga agcaatggct cgctacggca
    16201 gcgacaaacc ggatacacgt tttgatatgg aattgatcga tctaagcgat acagtcaaag
    16261 aagtagaatt caaagtcttc caaatggctc ttgaaaacgg cggtgtcgtg aaagcattga
    16321 atgctaaagg cgctgcagat cgctactcac gaaaagacat ggatcagtta ggtcaatatg
    16381 taggacaatt cggagctaag gggcttgcat ggctgaaagt agaagaagac ggattgaaag
    16441 gtccaattgc taaattcatg ggagaagcaa cagaagcaat cattaaagca acagatgcaa
    16501 aaccaggtga cttgctgatg tttggcgcag ataaaagtga aatcgtcgct gctgcattag
    16561 gtgctatccg gacacgttta ggaaaagaat taggactaat tgatgaatcg aaattcaatt
    16621 tcttatgggt aactgattgg ccacaatttg aattttcaga agaagaaggt cgatatgtct
    16681 ctgctcacca tccattcacg atgccaaaag aagaagatat cccgttattg gctgaagacc
    16741 ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa cggttatgaa ctaggcggag
    16801 gttctatccg tattcatact cgtgagcttc aagaaaaagt actagaaact ctaggcttta
    16861 caaaagaaca aatggaagaa caatttggtt tcttgttaac agctttagat tacggattcc
    16921 ctccacatgg cggtattgca ttaggattgg accgttttgc aatgttatta gccggagaag
    16981 ataatattcg tgaagtcatt gcctttccta aaaacggaaa agcagcagat gtgatgagtg
    17041 atgcaccaag tatggtttcg cctttacaat tgttcgaatt gaatattgat gtaacgaata
    17101 tagaagaata gccgataaaa aggtaaaaag gatttgtcga atacgcttct tttgacaacc
    17161 ttgatttaat tgaaaaacac tttataatga agccataaca tataaagcgg ggcttttcca
    17221 tgaaaaaaac attcgaaaat tgggcttatc atgattacac gaccaagctt tcggtatcta
    17281 tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt ttatcagcca ggacatattt
    17341 attcaagtgg tattactggt ctagctcaga ttttgacgac tttgtcaaaa tcactgatag
    17401 gattcgaagt accggtatca acaactttgt atttactgaa tatacctttg tttgtattgg
    17461 cgtggttaaa aatcgggaag aagtttacaa ttttcacttt tatcacagtc actttgacct
    17521 ccctttgtat ccatatttta ccggaaacag tcctatctac cgatccaatc atctgtgcgt
    17581 tgtttggggg ggctgtgatg ggactaggta ttgggctagc gttgagaaac ggcctatcat
    17641 cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa aacaggaaag tcgattggtt
    17701 ctatttcgat ctacttcaat gcattgatcg tttttgttgc cggaattcta tttggctggc
    17761 aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg aaaagtaact gatgtcgttt
    17821 atactaaaca aaagaagatg caggtaatga tcgtaacgaa acatccagaa aaagtagtaa
    17881 atgaaatcca aaactgtatg cgtagaggga tcacgatcat caatgaagca gaaggtgctt
    17941 ataagcatga taagcagaca gtattgatga cgatcgttac acgatacgaa ctgcctctac
    18001 ttagagaagc aatgaaagct tctgatccac aagcttttgt aagtattgca gaaaacgtcc
    18061 agatacttgg tcgtttctat gaggatggca tttagatcga gtttgacaaa aattcatgaa
    18121 atactgctgt agacagacgt tcgtttgtct gcagt
//
LOCUS       DACEMO010000036        18077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEMO010000036 DACEMO010000000
VERSION     DACEMO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.036"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(194..991)
                     /locus_tag="IW728_000947"
     CDS             complement(194..991)
                     /locus_tag="IW728_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1024322.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(988..2172)
                     /locus_tag="IW728_000948"
     CDS             complement(988..2172)
                     /locus_tag="IW728_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1024323.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2197..3444)
                     /locus_tag="IW728_000949"
     CDS             complement(2197..3444)
                     /locus_tag="IW728_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1024324.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3659..5404
                     /gene="dnaX"
                     /locus_tag="IW728_000950"
     CDS             3659..5404
                     /gene="dnaX"
                     /locus_tag="IW728_000950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1024325.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5424..5738
                     /locus_tag="IW728_000951"
     CDS             5424..5738
                     /locus_tag="IW728_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1024326.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5763..6359
                     /gene="recR"
                     /locus_tag="IW728_000952"
     CDS             5763..6359
                     /gene="recR"
                     /locus_tag="IW728_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1024327.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6423..7067
                     /locus_tag="IW728_000953"
     CDS             6423..7067
                     /locus_tag="IW728_000953"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1024328.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7082..7411
                     /locus_tag="IW728_000954"
     CDS             7082..7411
                     /locus_tag="IW728_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024329.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7425..8363
                     /gene="holB"
                     /locus_tag="IW728_000955"
     CDS             7425..8363
                     /gene="holB"
                     /locus_tag="IW728_000955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1024330.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8399..9223
                     /locus_tag="IW728_000956"
     CDS             8399..9223
                     /locus_tag="IW728_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1024331.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9216..9563
                     /locus_tag="IW728_000957"
     CDS             9216..9563
                     /locus_tag="IW728_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1024332.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9632..10504
                     /gene="rsmI"
                     /locus_tag="IW728_000958"
     CDS             9632..10504
                     /gene="rsmI"
                     /locus_tag="IW728_000958"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1024333.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10613..11734
                     /gene="dinB"
                     /locus_tag="IW728_000959"
     CDS             10613..11734
                     /gene="dinB"
                     /locus_tag="IW728_000959"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1024334.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11788..12390)
                     /gene="nrdG"
                     /locus_tag="IW728_000960"
     CDS             complement(11788..12390)
                     /gene="nrdG"
                     /locus_tag="IW728_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1024335.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12705..14894)
                     /gene="nrdD"
                     /locus_tag="IW728_000961"
     CDS             complement(12705..14894)
                     /gene="nrdD"
                     /locus_tag="IW728_000961"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1024336.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15220..15987
                     /locus_tag="IW728_000962"
     CDS             15220..15987
                     /locus_tag="IW728_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024337.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            15977..18019
                     /locus_tag="IW728_000963"
     CDS             15977..18019
                     /locus_tag="IW728_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024338.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         5634 a         3322 c         3759 g         5362 t
ORIGIN      
        1 caagaggttg tgaaaaagct gttctgcatc aagtaataag aagagccaag taaaaacagt
       61 ctcttatgtt tttaacttgg ctggacttat caccgtagat gtaagctttt gaacgccgtt
      121 tatttgcaac aaaaggggct gtgacacgat ttttgtcaca gccctttttt tgcgaacatt
      181 catttagcaa tttttagaaa cttctgccgc caccgccaaa agtcccgcca ccgctagaat
      241 gggtagtact tccgccaccg ccgcttccag gaggtgggct tttggggatt cttcttgtcg
      301 tgacaaaaga atttgtcaga cgatcggttt tttcagttaa tttgatactg gatttttctc
      361 ggaatggata tttgtagatg cctgatttca attgataacg cgaaacagtc acaaaataaa
      421 agacaagact aaggataaga gccaaaacaa cagcaatgat gatttctact gtcgtcaaaa
      481 ccttatagcg tgtaattttg ccagtttctt cgtctacacg ataatgtcct cctggaactc
      541 cgtcttccac atatgttgct gatttagaca ggtaagcttt tgcagcattg taataggcag
      601 catttgtcat ctgatcataa acatcatcta agatcgaatc gattctgcta tcagtcaaat
      661 aatcgatcat attaccagaa gtcgaaatgt agatttcacg ctgtcccata tctaatagca
      721 atactgagcc attttgatca tttccgactg cgtctcttaa gtaattatca gcaaagtctc
      781 ttggttcttc tgaattagat gtagtcgtta cgatcaagac tcgtcctttg attttttcat
      841 tcagcttttc tgcctgttgt tccaaagatt ggatttgatc tgttgtgaat aacccagcct
      901 catcatttac cgtagctgta tctgcatgag tagccaccgg ccagccaaca cttactagca
      961 aaagaaagaa taaaaaaaat tttttcatca tatcaagtaa cctccgatca gaaagaggat
     1021 cgctagccca gcgaaaatcc caagagcggt cagtcccaat tttttctggc tgatcggcag
     1081 gacaccgctg acctttcctg tttgtccatt cattgcgtag tagtagactt ttttacttga
     1141 gtcattgctt cgataagtaa caagccatac cggcagtaat acgtaattat ttacttcgtt
     1201 tgtgattgaa gcagaagttc gcacaccggt caatgttgta tatccatttg cggtatcttt
     1261 taacagcttc tctgaatagt cttttaattc agattgaatc tgtgatttga ttgcctcata
     1321 ttcgatatct cttttttctg cttggaaccc ggctaaatac tggcttttaa aatcaattgc
     1381 tttatctaac gggaaaggtt gcacgccttc gaccatcttt tgttgcgtat tttttgataa
     1441 ggcatttttt actagttctt taaaagaaag cttgcctgct cttgaaacag caaattgctt
     1501 cgtttctgtg tattcgatgt cacctatccg ccatatgcgg ataactgtac cattcgcctg
     1561 catggcacca tctacttcag catctgtagc ccaataaggg aaatacacac ctgtcaactt
     1621 atcgatttga tccttattga aaaaagcttt gggaacaaac cattttttct tggtccaagc
     1681 gagaaatctg ttgattgctt cttctttctc gacagcaaaa ggaagtactt tatttggcaa
     1741 gaattctccg cttaaacgtc ccgatagtac gacgggatta tggcagtagt aacaataagt
     1801 cgcagcagtt gtcgcatcag tcacgatctc tgcaccacag ttgggacaaa gaaaaagatc
     1861 tatcgctgcg gtattatctg cagattcgtc cgctgtatct gaaaaaccgc ctgcctcgtc
     1921 aaccgctttg cgctcggctt cattcatatc ggcaagctgt tcttgggctg taaaagtaaa
     1981 atcttcggta ggtgaaggtt cttctttgct ttcttcgcca gccatacgcg cttctttttg
     2041 cttttgctca tattggctga cttcttcttc tgtatagata ttcaggcagt attcgcaatg
     2101 aaatttttgg tctttgggat caaaagtcaa cggaccgccg caattaggac atttatgtgt
     2161 aagaacatcc atcagctgca cacctttcta aataaattaa ttaacgggga ttcctttaaa
     2221 cggtttacca cagtttggac agaatttagg gatgccgttg gaaagatcca cgatctcatt
     2281 gcattcactg catcgcattt gtagcttagg ctgaacgttt tctacaggtt tggcagctcc
     2341 gcagttggaa cagaattttc cggaattttc tgtcccgcaa actggacatg tccaagtatc
     2401 cgcatttcct tgtgcagttt gttgattttg tttttctgct tgttgtttga tttgttcttg
     2461 gttattttga gctgcttgag aaagataaga accatttgca ttcattccca tacctacacc
     2521 cataaagccg gtcattgctc ctgcttcatt ttttccggct gcttccatac cacgtgcaat
     2581 ggaaccttgc acgtatcctt cacgtacgct aggatcacct agcatcgccc cttcgttacg
     2641 catattgatc agtttcacag aatcatcggt gtaagaaatg ctggctacag ccacagaaac
     2701 aatctccatc ccacgtaatt cacgccaaga atcatccaat gcagtatcca tgtatttgct
     2761 taattccaaa ctcttagatg gtacataaga aatccgttga ccatctgcag acatctgatt
     2821 gattgctgat tgtagcgctg ttaggaattc agctaaatac tgctcattga tatcattgat
     2881 ttccacttgt gttttgtttt tagggattgc gtttgtataa aataaaatcg ggtcaacgac
     2941 atgaatagaa taagtgccat gtgcccgtaa gaataattct gcgttataga aattgtcaaa
     3001 gtagttcaac ggcgcgcttg taccgaattt gattcccttt atttcttgga gattgatata
     3061 aaaaacttgt tgtttctgcg gtgtgacgcc gccaaatttg aatcggtcaa aagtttcagc
     3121 aattgcttct ttcagtgttc cattgaacat ggaaggagct gcgtcatttt taatggtgta
     3181 ataaccttcc tcagcggtat aatcgatgat ttttccacca tcgaccaaaa gcatcatcat
     3241 gtttggatag acatggacga ctgttccgtc tgttaaaaca tcttctgttc ctttacgatt
     3301 ggagccacgt ttgtcatctt tgcggacttt gacacctttt gtcatgaccg ttgtatcact
     3361 catattgtct ggttcgatga cttctagcca ttgatctgcc agaccgccgc caaccatact
     3421 tgttgctgct ttgataagac ccataaaaaa ttcctcctct gattcagcta gtgccaaaca
     3481 attattgttt atttattata ccataggaag atgtgaaact tctcggacta gagacggaat
     3541 tgaaatggaa aattgcgtat tgtttaaata cctgaaaaag ccgagaagga tagtagtttt
     3601 tttacctaat atcacgtata ataagagata gtgaattttt taaaaaaggg ggatccttat
     3661 ggcatatcaa gcactttatc gagtctggcg ttcgcagcgt ttcgaagaca tcgttggaca
     3721 aaaagctgtg acacagacac taaaaaatgc tattgtttcc cacaaaacct cacatgccta
     3781 cttgtttaca ggacccagag gaaccggaaa gacgagtgca gcaaagattt tcgcaaaagc
     3841 aatcaactgt ccgaacagta aagacggtga gccatgcaac gaatgcgaaa tgtgtcgatc
     3901 aattactgct ggtacgcaag aagatgtgat cgaaatcgat gctgccagca ataacggagt
     3961 cgaagaaatc cgatttatcc gtgatcgggc aaattatgcg cctacaaagg cagcgtacaa
     4021 agtgtacatt attgatgagg tacatatgct ttcaacagga gctttcaatg ccttgttgaa
     4081 aacattggaa gagccaaaag aaagtgtcat attcatctta gcaacgaccg aacctcacaa
     4141 aataccagct acgatcattt cacggactca gcgatttgat tttaaacgga tcaatacggc
     4201 agatattgtc gaacatcttg aatttatctt gaaagaatcg actatcagct atgaaccagc
     4261 tgcattgaat gtgattgccc gttctgcaga aggcgggatg cgggatgcat tgagtattct
     4321 tgatcaggca atctcgttta gtgaaggaac aattacttta gatgatgcca tgcaagtgac
     4381 aggtagctta actgacgaaa tgatggacag ttacttgagt gcttgcgtga aaaaagaagt
     4441 accagaagca ttggaaacat tgaaccaaat tctcgcagcc ggcaaagaat cacgtcgctt
     4501 tctagaggat atgttacaat actgccgtga cttgctgatg tatcagcaag cacctaacct
     4561 agttgaggaa caatcgagta cgatcagaag tacatttaca gaattggcac aaactgtttc
     4621 agcggatcag ctgttttcaa tgataaagat tttgagcgat actcaaaatg aaattcgttt
     4681 taccaatagt gccgctattt atttggaagt cgctacagtc aaacttgcac agctaccgtc
     4741 tgcttctgtt ccttcagcag atgtagcatt tgcagatgat cagatagtaa aacagctaca
     4801 acaagagatt aaggaactaa gaaacgaaat caaggaacta cgcagtaata caggaattga
     4861 gaatacaaaa gtacagccgg tacagcaaac aaatcataaa aaacagacaa gtacctatcg
     4921 tgtaccgacg gaacgagtat ataaagttct gcgggaagcg acaagacagc atctgaacca
     4981 agtaaaagaa gtatgggaag acttgctgat gagtctatct gtcactcagc gagcaatgct
     5041 taaagcaagc gaaccagttg cagctggacc tacaggacta gtgattgcgt ttgattatga
     5101 gattgtttgt caaagagcag caaatgacga agaattgcag ctttcggtac acaacaactt
     5161 aagccgaatg atcaaagatt atgcgcctga tcctgtttat attacaagag aaagctggcc
     5221 agaacttcga agaaattatt tgacgcaggc acaagaagag ggaacagaaa actcttttgc
     5281 ttctgaagac tcagaaggag aaatcgagtt gcttcctcaa gaacaagaag taccagaaga
     5341 aacacttgta gtaaaagcag aggaactgtt tggctccgaa gctgttacag tggtagatga
     5401 ttaataaaaa ggagtagata attatgatgc gtggaatggg caatatgcaa ggcatgatga
     5461 aacaagtaca aaaaatgcaa aaagaaatgg cagaagcaca agagctgcta aataaaacag
     5521 agtttacagg taaagccacc aatgaattgg tcaaagcggt ttttacaggc gatcgtcgca
     5581 tgaaagatat ccaaatcaat gaagaagtcg ttgatcctga tgatatcgag atgcttcagg
     5641 atcttgtcgt aatggcagtc aatgatgcga tcgcacaaat tgatgtggaa actgagaaaa
     5701 aattagggaa atacgcaaaa ggtttgccag gattttagtt ttaaactgaa ggagtaatga
     5761 atatgcaata tccagaacca attgcgaaac tgatcgacag ctacatgaaa cttccaggaa
     5821 tcggtcagaa aacagcgaca agactcgcct tttatacgat cgatatgaaa gatgaagtag
     5881 taaatgaatt tgctaaatct ttgttaagtg taaaacgcga tttgcatttt tgtagtatct
     5941 gcggaaacat cacagaagaa gatccctgcg aaatatgtag aaatccttca cgtgatcgaa
     6001 gcgttatcct agtggtcgaa gagccgaaag atgtaatggc aatggaaaaa atgcgagaat
     6061 atcacggact ttatcatgtt ctccatggtg tgttatcacc gatggaaggg acaggtccag
     6121 aagacatcaa tatttcttct ctgttgcagc ggctgcatga tgcagatgta aaagaagtga
     6181 ttatcgcaac aaatgcaaca acagagggag aagcaacagc tatgtatctc tcacgtctga
     6241 tcaaacccgc aggtatcaaa gtgactcgtc tagcacatgg tctgtctgta ggctctgata
     6301 tcgagtatgc agatgaagtt actttattga aagctgtcga aggaagacgt gagttataaa
     6361 tttctaatta agtgtttttt cagatacaat ataaaagcag cacaagagat gggggcaaca
     6421 atgtgaacgg actatttatt acaatcgagg ggcctgatgg tgcaggaaaa accagcatct
     6481 tgaatgaact ttaccctctt ttgaaagaag tagcaaaaac agaaatcatt caaacaagag
     6541 aaccaggagg cattccgatt gctgaagaga tccgagcagt gatccttgat ccaaaaaatg
     6601 atcggatgga tgaacgtacg gaagcactgc tttatgcagc agcaagaaga caacatctag
     6661 tcgaaaaggt tctgcctgct ctggcaaaag ggaaaatcgt tttatgcgat agatttgtcg
     6721 acagttctct tgcttatcaa ggtgcaggcc gacgcatcgg cgttaccgag attgctcggt
     6781 tgaatgagtt tgctacagaa ggtactacgc cggattttac cctttatctt gacgtagatt
     6841 cagacaccgg attaagaaga atcaagaaaa acagacaaaa tcaaatcgac cgtttagatt
     6901 cggaaggttt ggaattccac cagcgtgtac gtcatgctta cttaaagctg gcagaagaga
     6961 atccggaaag aatccacaaa gttgatgcaa gaaaaagctt tgaagaagtg ctgcaaacaa
     7021 gttatcatac aattattgaa cagtatccgc aattttttga aaactaggaa ggtgaacgtt
     7081 tatgaaaatt attttagcga ttatccaaga caaagacagc aaccgtttat caaatgaatt
     7141 gattgatgca aatatccgtg ccacaaaact atcatcgaca ggcggcttcc ttaaggctgg
     7201 aaacagtacg tttatcgtgg gtattgaaga tgaccgtgta gaagaagcat tggaaatcat
     7261 caaaaaaacg tgtgaatcaa gaaagcaatt tgtatctaca cctgttacac tggatatttc
     7321 gatggatggg ggcgtccctt atccagtaga agtagaagta ggcggtgcca cagtcttcgt
     7381 cttacctgtt gaaggattcc atcaatttta ggaggagctg agctatgaat caagaaataa
     7441 acttggctca aatgcaaccg atcgtgtatc agcagcttca acggagtttt gagcatgggc
     7501 gtatcgctca tgcttatctt tttgaagggg agaaaggaac aggaaaacat gaagtaggta
     7561 tatggcttgc acagcacctg ttctgtaccc aaatgaaaga caatctgcct tgtggaaaat
     7621 gcaacaattg tcagcggatc gaaaagaaag aacatccaga tgttttggtt attgaaccgg
     7681 aaggacaaac catcaaagtc gatcagatca gacgtttgca gacggaattc agtcgcagcg
     7741 gttacgaatc aagaaaaaaa gtttttttga tcaaagaagc tgaaaaaatg aatagcagtg
     7801 cggcaaacag tttgttgaaa ttcttggaag agccccctgg agatttttta gcggtgttag
     7861 aaacagatgc agtaggaaga attctcccta ctattcaatc tcgttgtcaa gttctgcatt
     7921 ttcaagaact ttcgaaagaa gcactgatcc atcgattgca agaagagcag attccattgg
     7981 aaaaagcaaa acttttggct ttcttaacaa acagtttagg aaaagcagtt gaaatctctc
     8041 aagatgaatg gtttaatgat gctaaggact tgatttatca atggttcgtt tacttgcaaa
     8101 agcaagatac tcaagccttt atctatgtcc aaaaaaaatt ggtaaaatca tttaaagaaa
     8161 aaacacaaca gtttacagga ttatctgtct tgatgtttta ttatcaggaa gcacagcgtc
     8221 aagcagtagc aaacggcgaa tatggcaaag tgaaacagat aaaccagata attgaacgga
     8281 tattgatcgc agagcagaaa ctacgcagca atgtcagttt tcaggctgtt gcggaacagt
     8341 tcgttttgca tacgattttt tagcttgccg caaaggatga tgatcaagga ggatggaaat
     8401 ggtagaagta gtaggtgtcc gatatagaga tgcaggtcat atctattatt ttgcaccagg
     8461 agactctgaa tatgtttata gtgacaaggt attagtcgaa tctcagcaat ccttacatat
     8521 tgctactgtt gtccttccta aaaaagagat tgatcccaat gaccttcctg aagctatccg
     8581 tccgatcttg cataaagcgt cacaagctga attagaaaaa gacaaaaaaa acaaaaaaga
     8641 tgcttatgat gcattgaaaa tcgcaaaaga aaaagtgcag ttgcgtgagt tggaaatgaa
     8701 aatggtcaaa gccgaataca catttgatcg aaataaattg attttttctt tcacagcgga
     8761 cgggcgaatt gactttagag aattggtaaa agatctggcc gctgtatttc gtactcgtat
     8821 tgagcttcga caaattggtg tccgcgatga agcaaagata ataggcggaa ttggtccatg
     8881 tgggcgtcca ttatgctgtt cttctttttt aggcgatttc gtgcctgtat caatcaaaat
     8941 ggcaaaagat caaagtttat ctttgaatcc aacaaaaatt tccggtttat gcggacgctt
     9001 gatgtgctgt ctgcaatatg agagcgaaga atacgagaca gcgaaaaaag aactccctga
     9061 ctttggaaaa gtaattgcta cacctgatgg taaagggaaa gtcgtagggt tgaatctgct
     9121 tagccgtatc gttagtattc ggctgactgg acgggaaaat cctgtcgaat atgaatggga
     9181 tgaattgaaa gaatttatcc aagtaggtga gatgaatgga taagagatca ttatacgacg
     9241 gattgaatca attagaatca gagttgagaa atacattaac agaacttgtt gaaatgaagc
     9301 atgctttgca tgagatcgta gaaaaaaata caactttgga gatggaaaat caacgtctgc
     9361 gggaacacct gcgggaattg aaccaagttg cacaagctcc agctgaaaat gtgaagcagg
     9421 aactttctaa gtctcgtatg aatcttgaaa aaatttatga agaaggattc catgtctgct
     9481 acgatttata tggatcaaga agggacaatg acgagccttg tgcgttttgt ctagaagtga
     9541 tctatcgcga aagcggtcga tgaccaataa caagaggaga ctggagcgga gaaatactgc
     9601 tccagtcttt ttattaagga gattgtgaca gatgtataat caaaaaagtt tcaagggaaa
     9661 tccggtatat ggggatttat atcttgtacc gacgccaatt ggaaatttag aagatatgag
     9721 ctacagaagt gttcgtatgc tgcaagaatc cgatcttatc gcaagtgagg atacgcgtaa
     9781 tacacaaaag ttattgaatc attttgaaat ccatacaccc caaaaaagtt tacatgaaca
     9841 taattataaa gaacgtgtcc ctcaattgat tgctttgtta aaagaaggaa aaacgattgc
     9901 tcaagtcagt gacgcaggaa tgccttccat cagcgatcct ggacatgagc tagttttagc
     9961 ctgcataaaa gaagggattt ctgtgatctc tatcccagga cctacagctg ggatgacggc
    10021 tttgattgct tcagggctag tgcctcaacc atttttattt tatggcttcc ttccacgtaa
    10081 gaaaaaagaa cagaaagaaa cattgaaaaa gctaaaagaa cagacagtta cgctgatttt
    10141 ttatgagtca ccttatcgga tcactgctac attgacgaat ttcttagaaa tttttggaaa
    10201 tcgccaagtc gttttgtgtc gggaattgac aaaggtccat gaagaatacc ttagaggaag
    10261 tacggaagag ctactcgctt atatcgaaga acatccagta aagggtgaat gttgtctgct
    10321 tgtagaaggg aatttgttcg aaatgccagt gacggaagtt gatgaagagt tgggaacgtt
    10381 aaaggaacaa gtagaagcaa agatcgcttc aggcgaaaaa ccaaatgctg ctatcaaagc
    10441 agtagcgctg aaaaatgggc tgaaaaaaca agaagtctac cgtcagtacc atgaattaga
    10501 ctgagatagt ataagttgtc tataatgaaa gactaatgag gaaatcagct actaactttt
    10561 caaatctctt gaacgcgaac gaatgttctt gtataatgaa taagaggtga ggatgatgga
    10621 gctgcaattt cctttacata atgatacatc aagaaaaatc attcatattg atatggatgc
    10681 ttttttcgct tcggttgaag aacgagatca tcctgagttt cgcggacatc cattagtaat
    10741 tgccagacac cccagtgaca ctggtgggcg tggagtagtg actacagcaa attatgaagc
    10801 aagaaaattc ggaattcatt cagcaatgag tgcacaaaaa gcgtatgagc tttgtccgca
    10861 agccatcttt aagcctggcg accatcataa atacagcgat atttccaaac aagtacggga
    10921 agtattctat cgttatacag atattgtgga accagttagt atcgatgaag cgtatcttga
    10981 tgtgaccgaa aataaaatca attgcaagtc agccatcaaa atcgcccgaa tgatccaagc
    11041 agatatttgg gagagtgtcc agctaacatg ttctgcggga gtaagttaca ataagttttt
    11101 ggccaaatta gcatcagact atcaaaaacc tagaggaatc acggtagttg cccctcaaga
    11161 cgctgtgcca tttttaaaag ctttgccaat tgaaaaattt catggggtag ggaagaaaac
    11221 tgtacctaga atgcatgagc tagggattta tacaggaaaa gacctatatg aatgcacaga
    11281 aatgatgttg attcgaaatt ttggaaaaat gggctattct ctttatcgta aagtcagagg
    11341 aatccatgat tctccagtaa acgttacaag agagcgaaaa tctgtgggaa aagagcatac
    11401 ttatgggcag ccgttgcaaa cggaagaagc agtgctgact cagttgcgcc aactagctga
    11461 aaaagtagaa gaagcactga gaagagtcca aaaacatggg aaaacagtag ttttgaaggt
    11521 caggtacacg gactattcta ctgtgaccaa aagagtgaca ttgccagaat atatctataa
    11581 aaaagaagca ctcttttatc aagcaagcct tatatgggag gaaatcttgg gagtagaaaa
    11641 aggaatccga ttgttaggga ttactttgac gaatctggac ccgatgactt atgaaaacat
    11701 cgtccttcca ttatgggaaa atcaagaaat ataaaaacag ctttgagagt tgaaaccgga
    11761 aatccgtttt ctctcccaaa gctgttttta tatcaagtct tgctttttga tttgttcata
    11821 ggtttctgtt gcatccgtac atttttccca aatcacaact tgattggatt ctaaagattt
    11881 ttggacgtca atgatccgct ggttgctact tccgcgaaat tgtagtttta ggtcacgttt
    11941 gcttaattca aagcgtccgt caacaaggat atcgatttgc gaaaggagtt ctaatttatc
    12001 ttctgaatct tgtagcaatt catcaaaggt atagcctgtc catgaccaga tatcttttgt
    12061 cgtaccgaac tcttttcgga tacgtcgtac gacttgcaga caaacatctg tattcaaaaa
    12121 aggttcgcca ccaagtaaag ttaatccttg cacgtaatcg ttgcgcaagt cagcgatgat
    12181 ttgttcttct aattcaggtg tgaatggttt gccatatcgg aaattttgtg cagctttgtt
    12241 gaagcagcct tcgcaggcaa ataagcagcc actaacgtaa agactgttgc ggacaccttc
    12301 accatcgaca aaattaaacg gtttgtagtc agcaatatag ttttggctgt agtcttttgc
    12361 taaccattct tttggttttg gatttctcat aaaaaaactt ctttcattta aggttcaaag
    12421 atatagctat tgaagataaa atctactcga ccacataaat aagaacaaac tgacctaaat
    12481 ccattgttca agaaagcttg ttcttatttt gatagagggc gttcaaagat acaaagccat
    12541 cgaaagaggg tggtgaaaaa agacttgtca tagcctctta ttctgaataa acggtgttca
    12601 aaagcttgca tctgcagtaa taagcccaac taaacgaaaa atatgaggaa ctatttttgt
    12661 ttagctattc ttattacttg atgcaggaca gctttttcac aacctcattt catgtgtttc
    12721 acacgtgagg aaatttcttt atggcggcca tgtaccatcg gacgagcttg cggatttcct
    12781 aaatagccac aagtacgttt gactacatca catgtttttg gatcatgatt tccacattct
    12841 ggacattcga accctcgttc tgtcggtttg aaatcacctt cgaaaccaca ttgatagcaa
    12901 tgatcgattg gcgtattcgt accaagatac ccgattttgt catatgcaaa atcccataca
    12961 gcttctaaag ctttcggatt ttgttggagc atagggtatt cacagtagtg gatgaatcca
    13021 cctgaacaat attttggata atccttctca aattccaatt tttcaaatgg cgttgggttt
    13081 ttacgcacat catagtggaa actgtttgta taatattctt tatctgtgat attttcgatg
    13141 atgccgaatt tttctgtatc caaacggcag aaacggtcag ttagactttc acttggagtt
    13201 gaatagacac tgaaatgata accatattca tccccccatg catctgcatt agcttttaat
    13261 tctttgacga tatcaagtgt gaaagctttt gcttctgggt ttgtttccca ttcgccaccg
    13321 aagaaagctg aagcaacttc atacaatcca atgtagccta acgatacagt agctcgacgg
    13381 tttttgaata attcattgac agaatcggta cgtgaaagac gtttcccgaa agcaccatac
    13441 atatataaaa ttggcgcatt tgctggaatg gcttctttac aacgttctac acgatagaga
    13501 agcgcatctt tcatgatttc taagcggtca gacagtaact gccagaattt attctgatct
    13561 ccttttgatt cgatcgcgat tcttggcaaa ttcaatgtaa caacacctaa attcattcgt
    13621 ccgacattta cttcttgacc attttcgtct ttccaccctt gcaagaatga acgacagccc
    13681 ataggtactt tgaaacttcc agttaattca acgattttat cgtaattcag aatatcagga
    13741 tacattcttt tcgttgcaca ttccaatgct agttgtttga tgtcgtaatt aggatcttca
    13801 gggtttaagt tcacaccttt tttcaaagtg aagatcagtt tagggaagat agctgtccgc
    13861 ttttcactcc ctaatccatt gattcgaatc tgtaaaattg ctttttgaat ttctctttca
    13921 aaccagtttt ctcctagtcc aaatcctaat gaagtgaaag gagtttgtcc atttgaagta
    13981 aataaggtat tgatttcata ttccaaactt tgcatagcat cgaagatatc ttttttggtt
    14041 tttgcttttg catattcttt ttggcgttct ggcgaatcga tccattcttc cgcgtctttt
    14101 agatgttttt tatagttcaa ttctgcaaaa ggagcaagca attcatctgt tcggtcggca
    14161 gaacagccac catattgact tgacgctaca ttggcaataa tctgtgagat ttgtgctgtg
    14221 gcggtctgga tagattttgg cgattcgact tcggcattgc cgattttgaa cccattgttc
    14281 agcatgcctt tgaaatcaat caagcagcaa ttcgtcatcg gtgtataagg atgataatcc
    14341 aaatcatggt aatggatatc gcctttttga tgcgcatttg ctatatgtgg tggcaacatt
    14401 ttcagtccga ttgattttcc aacgatcccg gctgtcagat ctcgctgtgt attgaaaaca
    14461 ttactgtctt tatttgcgtt ttcgttcacg acagtttggt ccttgttgat tagcttttca
    14521 atcgaaaagt tgatatctgt tgctttactg cgagcaaaat ctcgtttggt acgataattg
    14581 atgtactctt ctgctaattc atattcgttt ttttctagta atatatgttc cacgatgttt
    14641 tgaatctcat agattttgac attttcagca aaccggcttt tgatttcttg gacaaccaag
    14701 tctgcaatca tttgaatttt ttcataagta agtggtgata atgagccgtg aattttcttt
    14761 tctgctttga tcagtgcttc attgatcttt tgttcatcga aatcaacatg gcggccatcg
    14821 cgtttgatga ctttcatctg ggatgtctct ggctgctgag taacgacagt tttttcttca
    14881 taatttactt tcattatctt caactcctgc taaattgttc ttgtctatca taaaacaaaa
    14941 tcggggaagg agcaactaaa tatcggctat atatagtatt caatgtgctg tataattgga
    15001 tttctatact atatgtagtg tatattttat tgtgaaaaaa ggtgaaaact tgtagctggt
    15061 ggatttgttt tttgcatatt taattttgct gaataaaata attgaataaa attttttatt
    15121 cagtccaagt aaaagtgaca aaaacataag agcaaattat tcgtagatat gctactatgc
    15181 aaataaatag aaatgaaaaa tgaaagagga ggagaagaat tggaaaagat gatcgaagtg
    15241 aaacatctaa gtaaaagcta cggcagcaaa ctttctcccg tcaaagcctt agataatatc
    15301 tcattcacag tagaagaagg cgagtttgtt gggattatgg gcccaagtgg tgcaggaaag
    15361 acaacgctga tgaatatctt gtccacgatc aatttgccta cgatgggcag tgtctcgatc
    15421 caaggaaaag aaatcacaaa aatgaaaaac agtgagctca gtgactttcg tcgaaaaaaa
    15481 ctaggattca tttttcaaga gttcaatttg atcgataccc taaatgcaaa agacaatatc
    15541 ctattgcccc tagcagttga acggatgact aaagaggaaa tggaaaagag agtaagacat
    15601 gtagctcaat tgttgaatat agaagaactg ctcaaacggt atcccgatga actttctgtc
    15661 ggacagagac aacgaattgc agctgctaga gcacttgttg ttcagccaca agtgattttt
    15721 gcagatgaac caactggctc tttggactcg aagtcggcta cagaattgtt gaactacttg
    15781 aagaccatga atcaaaaaga aaaagcaacg atactgttag tcacccatga tccatataca
    15841 gcaagctact gcgatagaat tttatttatt aaagatggtg tgattttttc tgaagttgtc
    15901 agaagaggga caagaagaga gttctttgag aaagtaatag acatgcaggc gacgattggc
    15961 ggaggaggaa aaatgaatgc tgtataaact agccgttaaa agtttttttc ggcagtcacg
    16021 aggctatctt gtttattttt tcagtttgac tttatccgta atgatctatt attccttcag
    16081 cgcaatgacc tacgatcaat ctttagtcag aagagcaagt caagatactt cgatagatgg
    16141 tggattgagt tttggtggat ttatcattac tgttgttttg ctttttttcg tattcagtgc
    16201 caaccgattt ttcttgaata gaaggcaaaa ggaaataggc atctatcagc tttttggtat
    16261 gaataaactg cagatatcag gtatatatgt gttagaaatc atgataattg gattgttcgc
    16321 ctgtatttct gggatattac tcggtattat tttttccaaa ctcttttcaa tgattttggt
    16381 tcgaatgatg gatatggatc ttactagtcc attttttatc tccattccat cagtagtaga
    16441 tactttattt gcttttttca tcatcctttt agcagtatct gtatattcaa tatggaaaat
    16501 atggcgctat ccaatgattc gaagttttgg agaaaaagaa caagcagata gttcaacaat
    16561 gcgcttcagg acaaggcacc gattactcgc agtcatcggg ttactacttt taacgagcgg
    16621 atattttgga gctagtcatt ttagggaaat cacttcatgg atgatcagtt atcattatct
    16681 tgatacatta gttttctttt ttcctttttt aatttttttc gtttgtgtgg tcggtactta
    16741 tttgtttttc tgttatacat ttcggttatt tttgatcata ttgagtaaat ggaagattta
    16801 ttactatggt atcaattttt tgataatggg taacacgcaa gttcatttgc tcaaaagttg
    16861 gcgtacaaat tctctgatta cagtcattct cggaatttct ttaatgacaa ttggcggcac
    16921 ggctagtatc tctactattt tatcctacca ggagaaagtt tcctccccga tggactatca
    16981 gtttgatgtt gagacagaga aaaaaatcaa gccgattcta gcagaggaag ggcagaagat
    17041 aactaaagaa atcaccttgc attataaagt cattgggagt atctatagtc aaaatctcga
    17101 agaacctatg tttcaaactg ctaatctgat cacggaaaca caatatcaag ccttcagaaa
    17161 agttaatcca gatttgccga acatttcttt gaaatcacct ggtcatactg ttttgcttga
    17221 tcaaatgcaa actctttttc ggaatatttc attatatgga tctagtattg gactgccaga
    17281 taatcaagaa ttgaagattc aacaaatgct tccaagtgta ttaggtgacg aaacaatggt
    17341 atatctaagt cctgtactga ttgtagatga taccgttttt gaaaaagcaa aaggtgtgga
    17401 ttatcaagta gtcaatgtag atgtatcagg tggaaataat gaaaaaatag atgaacgact
    17461 gagcaatgac ctgtcgataa agtggctcaa tgctgtttat tattcttatg atatcgtaaa
    17521 tggaagtcta caaggagaaa ttagcacaaa gaaacttccg gatgaccaaa tgaaggaagc
    17581 acaatttagc caagaaagct ctcgtttgaa tttctcttcc agctattcga aattgcgtgc
    17641 agttaatcgg cgaatcggta tcaatatttt tgtcacacta tttgtcggga tgatcgtgat
    17701 tattgctact ggtagtattt tgacagtccg gcaattagca gaagcagagg aagaaaaaga
    17761 aaattatcac ctattgacta agctggggat tccacaaaga aggatcagac ggatgatctt
    17821 cgagcaaaac gcacttacct tctttccacc catgatatta gggatcactc acgcagtatt
    17881 tgcaatcaat gtcttcactc agtatataaa aactgccgat tactggttgg cttatcttgt
    17941 gagtggctta ttgatcctta tctatctttt gctttatgtc attacgagtc agttatattg
    18001 tcggattatc gaggaatgat atacggaata agacaagata ataaatagaa ctaaattttg
    18061 aataccattt atttgga
//
LOCUS       DACEMO010000037        17897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEMO010000037 DACEMO010000000
VERSION     DACEMO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.037"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..182)
                     /locus_tag="IW728_000964"
     CDS             complement(<1..182)
                     /locus_tag="IW728_000964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024339.1"
                     /translation="MKDQKTNNKTGERSACSWAFTGAGLLHSGIYTAAALGLSLTGVG
                     AAVIPFAVGTAYGIAGA"
     gene            complement(196..534)
                     /locus_tag="IW728_000965"
     CDS             complement(196..534)
                     /locus_tag="IW728_000965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024340.1"
                     /translation="MKRIVKIFGILGVFLIGLSTFVSAGISVDASETNNFLVKQQETE
                     INIIQNNLATHKDSKGNRLVYIIDESDLKSSLDRINSEITVSEIQQYLNEFNRSIQKK
                     WPWMANRSAS"
     gene            complement(578..910)
                     /locus_tag="IW728_000966"
     CDS             complement(578..910)
                     /locus_tag="IW728_000966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024341.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRKEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLVWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(924..1154)
                     /locus_tag="IW728_000967"
     CDS             complement(924..1154)
                     /locus_tag="IW728_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024342.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKVYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1376..1912)
                     /locus_tag="IW728_000968"
     CDS             complement(1376..1912)
                     /locus_tag="IW728_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024343.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLTTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDYMT
                     DRCIKDNSIDVDPHVQMKTTGKLHQVTQDIENPDQYLLFGEETTNEVTQFVLLQLKVD
                     QEAQKIDAIKIYYVRSVY"
     gene            complement(1933..3024)
                     /locus_tag="IW728_000969"
     CDS             complement(1933..3024)
                     /locus_tag="IW728_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1024344.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNWE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESTYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKDCYDYYGFLVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSDGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3032..5242)
                     /locus_tag="IW728_000970"
     CDS             complement(3032..5242)
                     /locus_tag="IW728_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024345.1"
                     /translation="MAYIKRKEKKWCVWLFLTVGFGLFFVGGLVYAVEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPAFDYMIMNTLKSFLSLMFQKSFYSVIILVAFLIY
                     NIVDDLILMNTVFGFLGKMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGGQVINPFELGGRSNQDLPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIHQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQRQIQRSSESKPSISVP
                     KSLQPAFSSFNNPVEQSKKIEKKNETLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5258..7786)
                     /locus_tag="IW728_000971"
     CDS             complement(5258..7786)
                     /locus_tag="IW728_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1024346.1"
                     /translation="MVLVKEPVKALKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFYDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRMPKEHKGLLQLEDELGTSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTTIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAIGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQEPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQIEEQDTLIMQ"
     gene            complement(7840..8250)
                     /locus_tag="IW728_000972"
     CDS             complement(7840..8250)
                     /locus_tag="IW728_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024347.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFFIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFANKYVYLED"
     gene            complement(8263..8481)
                     /locus_tag="IW728_000973"
     CDS             complement(8263..8481)
                     /locus_tag="IW728_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024348.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8478..9470)
                     /locus_tag="IW728_000974"
     CDS             complement(8478..9470)
                     /locus_tag="IW728_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1024349.1"
                     /translation="MKKRQLRRKEKVYTEKVPNQRKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSQSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQEILKKEFYAQNFIQEPGSVSGIRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMDPENKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKTFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDRKGDERK"
     gene            complement(9549..10460)
                     /locus_tag="IW728_000975"
     CDS             complement(9549..10460)
                     /locus_tag="IW728_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1024350.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLSDVETELNGGTTEKPAEPEHLQPEKPEKEPVAPEPEIPESTLPTDPIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSETNTDSSSTEQPTMSFTSEEPKEEPKQPAEKSEAIPK
                     ENKPIVPAKEVTVSVTPNGEITTNNSQGMSVPIVTSNVEELTHIPTPTTPLKAEGGQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVLS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10479..10823)
                     /locus_tag="IW728_000976"
     CDS             complement(10479..10823)
                     /locus_tag="IW728_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024351.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLGPRTERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10843..11400)
                     /locus_tag="IW728_000977"
     CDS             complement(10843..11400)
                     /locus_tag="IW728_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024352.1"
                     /translation="MSLEFLGEPIEKEEFVSHYMDLFIRVIKKLCTIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFHKREKNQMSAEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEKLMMDFSDLYRGYECSVIQER"
     gene            complement(11480..11980)
                     /locus_tag="IW728_000978"
     CDS             complement(11480..11980)
                     /locus_tag="IW728_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024353.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPKEYPIEKRELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPINFLKKC
                     HLRILP"
     gene            complement(11981..12526)
                     /locus_tag="IW728_000979"
     CDS             complement(11981..12526)
                     /locus_tag="IW728_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024354.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGTLKFIRFYRFSLPVPAILAVK
                     KGCDKMKIYENCVAALSNEKPLVEEISLATVTWEMNRQPTEEQTYLSLEEIELELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12523..13779)
                     /locus_tag="IW728_000980"
     CDS             complement(12523..13779)
                     /locus_tag="IW728_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1024355.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKKKIPEDFEKRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKIVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKQTVTQNVLVQFSGQGCREYEEVMREQRTD
                     WRTWLYSIWKNQGKLEAGYKRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKKFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISEIEAL
                     TKYGIYNRYELRLANEKATQAVEAFILDHSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIENAELNDEQQSYLEWMGTSGGKIA"
     gene            complement(14142..14456)
                     /locus_tag="IW728_000981"
     CDS             complement(14142..14456)
                     /locus_tag="IW728_000981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024356.1"
                     /translation="MGEVLYRVSSAAGEISPDFAVRRLYEWINKVEYYTKGSYVFRRI
                     ERETLFVTRNQIVLTKEDILRFHQVYRLCKEKNIQLRLAILQCFAPEQYKELQEKEDS
                     LV"
     gene            complement(14583..15776)
                     /locus_tag="IW728_000982"
                     /pseudo
     CDS             complement(14583..15776)
                     /locus_tag="IW728_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            complement(15966..16322)
                     /locus_tag="IW728_000983"
     CDS             complement(15966..16322)
                     /locus_tag="IW728_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024357.1"
                     /translation="MNLDKTNQTLREIMVCCQNIQQLIEYFGDNPTIFIQTNEYICAV
                     IGQCMFLRDLVRFSLPYQWKQDHQEVVWIRVVRICNMCAHPYKNRLNLFRLWFFIKCD
                     IPVLVAFCQKFLAQEK"
     gene            complement(16388..16840)
                     /locus_tag="IW728_000984"
     CDS             complement(16388..16840)
                     /locus_tag="IW728_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024358.1"
                     /translation="MGLKFPNNYDITEALDETCIGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNSNPKQSQEKGLDQKQPSADSKEKEKK"
     gene            complement(16842..17207)
                     /locus_tag="IW728_000985"
     CDS             complement(16842..17207)
                     /locus_tag="IW728_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024359.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            complement(17224..17418)
                     /locus_tag="IW728_000986"
     CDS             complement(17224..17418)
                     /locus_tag="IW728_000986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024360.1"
                     /translation="MEKKNINICFLSGKECTYEECTDISVHERDGMLIVDMSPSDEPF
                     VVKHYFPLCNIESFRIDCEF"
     gene            complement(17435..17680)
                     /locus_tag="IW728_000987"
     CDS             complement(17435..17680)
                     /locus_tag="IW728_000987"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024361.1"
                     /translation="MEEIFFCMMVEFLFVSIGFLIMGEAIMIYLFPFDSKDECIFSSI
                     IIFFILACVWILAREIYHQFVLEDGKGIITWFLQKRK"
BASE COUNT         5434 a         3901 c         2540 g         6022 t
ORIGIN      
        1 gctcctgcga ttccataggc tgttcctaca gcaaatggaa taacggctgc acctactccc
       61 gtaaggctta aacctagagc agcagctgta taaatcccag aatgtagaag tccagcccca
      121 gtgaaagccc aactgcaggc acttcgttca ccagttttat tatttgtttt ttgatctttc
      181 atataattct caacttcatg aagctgatcg gttagccatc catggccatt ttttttgaat
      241 agaacgatta aattcattta gatactgttg gatttcagaa actgtaattt cagaattaat
      301 tctatctaaa cttgatttta aatcactctc atcaataata tatactaatc gatttccttt
      361 agaatcttta tgtgtggcta aattattttg aatgatattt atttcagttt cttgttgttt
      421 tactaaaaaa ttatttgttt cagaagcatc gacacttatt ccagcgctaa caaaagtact
      481 taaacctatt aagaaaacac ctaaaatacc aaatatttta actattcttt tcataatttt
      541 tcctacttaa aaataagtta tttttaaaat atcttgatta tgaattcatc ccccaaaggc
      601 cttttaagat tgatacagca gtccgttcac ttcttttaga cttgtcatat aagtctttta
      661 aatgatccca taccagacta gtttgtctat ctagcttcaa tccattaaac gctaatgaac
      721 ggttcatttt ttctattact ttagataaag ctacagaaat ttttatacct tcacaacgcg
      781 catcttggat ttcaataata gtatcatcaa ttagtttttg aatttctttc ctatcaacat
      841 attcaatctt agaaagagaa tcttttaaag ctactaatga ttcaacgatt tcttttttct
      901 cttcagacat tctcttcctc cttctagtga cccgtaaagc catgtagagc agagtccatt
      961 gaaccagtaa ttacactatc tacaacgcca ttccaattta ctctacattt tccatttttc
     1021 agatttgtcg gtccatacgg atagactttt tgacattcaa tgttcggatt ataccagata
     1081 ggattatacc attcagccgc agaagcagat tcagtcgata caaacaatcc cacaccaaca
     1141 aaaccaatac acatcacact atagccaatt aattttttca tctcgtatcc atctcctaaa
     1201 aaatagaata gttcttgtag ttattcataa gacatatttt ttccgttttt gttttatcct
     1261 ttctgtccta taacaacttt tttcaagtaa agaatgacat acttcttctt aaaaaaacag
     1321 tctgtttttt tctatttttt cggtgcataa taaatatgag tgttcatttt ttattttaat
     1381 acacacttcg tacataataa atcttgatcg catcaatctt ttgagcttct tgatctactt
     1441 tcaattgaag taatacaaat tgagtgacct cattcgttgt ttcttctcca aataacaaat
     1501 attgatccgg attctctata tcttgtgtca cctggtgaag tttcccagtt gttttcattt
     1561 gtacatgcgg gtctacatca atactattgt ctttaataca acgatccgtc atatagtccc
     1621 tcacagcttg attgcgttta taaacatctg tgaaattact ccattttcta gcaaaatctt
     1681 ttaagaaatt ttttgtcatt tcatctattt gttgcgctgt ctcgtcttct aatgaaccaa
     1741 tttgactgtt actttcagta ctttgaagag ttccacttgt ttcttttact gcttcgattg
     1801 tttcttctgt cgttgtcaat tgtgtttttt gtttttcaga agaaaaccaa tgacaaccag
     1861 taagacctat caaacataac caactaaata ctaaaattat tccttttttc acgcttctag
     1921 acactgcctt tcttatttca ccttacgaac cagactacga atttttgttt gattgtacgt
     1981 gcgttgatac cgagccataa tccgtccttg ctcggcattc tgttccacac tagagaaatg
     2041 ttgtccgcca tctgaaacat ctgttataat tccggtgtga ccatagattc caggagacaa
     2101 tgcaccacct gcttgccaat tcaccacatc tcctgcttgt aaatcacttg gttttgggtt
     2161 catgatcact tgccaaccat aggctgtcca atcataatct tcccctatta acatcgcaaa
     2221 catgtgtcca gaacccatta actgtgggcc acctagttgg gatacatacc acgccgtgcc
     2281 accataacac tctccattat ttaccgatcg tccaatgatc cgatctaaaa ctttcgcatt
     2341 acctttactt ttccctgcaa caagtgacga tttttttgtt ggtaggctat tccaatgttt
     2401 gatcgcaaaa gtatcatatt ctgttaagtt ataagacttt atgatactca tcgctccttg
     2461 gtaatacgga ctgccttcgg taatacctgc cgccgcatag cctccacgac ctagaatcaa
     2521 taaacaactc ccaggatctt tattgcccac acattgatta aagttgcttt gactcgccac
     2581 caaaaagccg taatagtcat aacaatcttt caggttcgga aacttcatat agaagccacc
     2641 ttcagaacca ccaatccccc gtgcagaacc ttgtggatag ggacaaccag gaaaccaggt
     2701 aatcccaaag aagtttgtat cgtttcttcc agattgtgat tgtccataag tcgattcata
     2761 acctaattga gaaagaatca cacttggtaa taactgatac tttaccgctg caccgactgc
     2821 tgcttgcgca tgttcatctg tgatcggacc acgtccttta atctctgaaa aaatcggtaa
     2881 tccttcggta tattcccaat taaaagcgac agctagacct gtagcctctc ctccatcctc
     2941 ggaaagttct cctgaaaaaa agaatggcaa tagaaaaaag ggaagaataa agaaaagcag
     3001 acaacctaga caccctttct tcatttcttc ctcatccttt cttcgataat ggtttttgaa
     3061 ctgttcgtcc ttttggtaaa gtaaaatcgg ttcctggttt tgcgtccaaa atcggcaatg
     3121 tttcattttt cttttctatt tttttcgatt gctctactgg attattaaaa gaagaaaatg
     3181 caggttgtaa ggattttggt acggaaatcg acggtttact ttcagacgaa cgttgtatct
     3241 gtcgttgcac agaacgattt cctgatagag tggctaccga cgacttagga acagagtatt
     3301 gattcaataa ctgctgcatt gctggataac tcccattttt cgcctgattt ttcacttggt
     3361 gaatctgttg tgaaagagaa ccagcagctg ttaattgtgg cacgggtact tttccagcag
     3421 gcaacggaat cgattgacca acaaaagcga tacctttatt taacaatggt tttctttgat
     3481 tattcccttg ttgcttactc gattgtgcca acgattgtac ctgttgtttc ggtcttactg
     3541 aattcatgga cgaatttgga atcaaaccat ttgaaagacc agaattccac tctgtttttg
     3601 atttggtaga agacatttca ggctctaccc catgataacg aacatgacga ccactataag
     3661 gcaagtcttg atttgaacgc ccacctagtt caaagggatt aatcacttgc ccccctttta
     3721 ccgattcttg tggtgtacgt ttttgttgaa acttttgaaa tccatgtatc gctgcagaag
     3781 cgagtggatt cgttgctcgg tacacattcc ctgctaatcc tgcacctttg attgccaaat
     3841 tttgcccagc agttgatagt tgaccaggca gttgtttcat ttgagacaaa gaatgacttg
     3901 ctatgttttt cgtgttattt atggtttgat ctgcagctcc taaccctagt ttctttaata
     3961 aatctttccg ttttacaatg agtaaaattg ataggagtgc tttgacaaac atttttccta
     4021 aaaaaccaaa aacagtattc attagaatta aatcgtctac tatgttatag atcaaaaatg
     4081 ccaccaaaat aatcacactg taaaaagatt tttgaaacat caagctcaaa aaggacttta
     4141 aggtattcat gatcatataa tcaaatgctg gaacaaaaga agcgagtaat gaaaaaggaa
     4201 tcacaagtaa taaacacaac accaacattt gaaagataaa acgaatcatg ccaatcacta
     4261 caagtggtaa tccaatactt agattaagca atggcaccat agaaccaacg actgctttat
     4321 aaacagcttt agttggttga atatagacac gataagcctt ctttttccct ctatcttctc
     4381 ctgcagcagt ggtcactttt gtctcaatat cagataattc ttcacttgac gcttccttgg
     4441 ctagaaattc ttgtggttca atctctgctt gatacaattc ttctaaattt gttgtcccat
     4501 aatttaacaa tagatacgga tcaacaacag ccgctttata ataaagttgg cgcacttttt
     4561 gaatggcttc cttttgtgtc gttggcacta aaacaccatt gacatctttt gtcgccaatt
     4621 catctagact atttgtagca gaaaaaataa agccttctac ctccacggac aaatcattta
     4681 accctttagt aaaagcttct cctttactag gtacgttact accatagcca aaccaaacca
     4741 ttgaaaaaat gacaacaaag agaaatcgaa taaacaccat ttttgcgtaa tgtttccctc
     4801 taaatatacg aagagtaaat agatataaca catacaagac gacaatcccc gcaccaatag
     4861 gatcgtatat tttcgaataa atagtatccg aaaaagtgaa aaactggtga attaactcgt
     4921 tcattgcagc tccttcatag agcttctcaa tgacaaaatc aaggccttgc caaatcaatt
     4981 tacttaaact aaagaacata ttcgtcatca tttttagaaa atctgtgcca tttgctccaa
     5041 ataacatttt gcctaatgaa aattcactat cagataaata agaagaaaag gtatcaatat
     5101 gaaatttccc gatctcatag atcgattgat ccacctgttt ttctaccgca tataccaatc
     5161 caccaacaaa aaacagtccg aatccaaccg ttaaaaaaag ccaaacgcac catttctttt
     5221 ccttacgctt tatataagcc attgtttcac ctcgtttcta ttgcatgatt aacgtatcct
     5281 gttcttctat ttgagagtga cttgtttttt ccgttgtttg taatgcagcg gtcatttctt
     5341 caaacaaact gtgtacattg agttttccta cacgtccata gggatctaac atccaacact
     5401 gtcctttaat catatggctc atttcagata agttcgcttc atttacttcc aaacgtaaat
     5461 gagtcaaaat cttttctcgc tcactgtctt catcaaaagc gaagacaaca ccaaaattcc
     5521 ctttttcttt atctgaacct aagtcgccaa taaattgtgt atctagcact aattgattca
     5581 tctgtgaacg ccccacacgc gccattgctg caataatact acgtcccgcg ttcgaattat
     5641 taaataccca tgcttctgta aaataaatcg ctgttttctt actactatct cgttttccaa
     5701 acatttcaca aaaacgacct aaactaatca tcaaacacaa agactttcga tccgcatctg
     5761 tatatagttc tggtcggaca ttgtcatttg gtaatttcaa atcttggatc tctaaaatcg
     5821 ttgttttggc attaaagtct aatcccgggt tttctccata agaaaatcct aaacgtaaaa
     5881 tcgaatcttc taccgtcaag cgtaaaaact caccatattc tcgaacctct ttttgttcca
     5941 tttgttctaa atactcgacc acatgcatca agccaacttg ttgtccggct tctttttgaa
     6001 ctaaaacttc ttggattccc tttaataccg ccccttttaa aaggtgattg gtacctaacg
     6061 gacttaactc atcaatcata tcctgggcta cttgtttcgc atctactcgg gataaacaaa
     6121 caattggatc taatacgcca taattacggg gatttgtagc atctaacgtg acataactca
     6181 ttttttgcaa atgttccaca aacaaagggt aatgtttctg ataatacggc tcttgaatga
     6241 gtttcgtaaa ccatttgcgc ttttctcctt tgggatccac aaataaactt tgtacattca
     6301 taaacgaact atacaaaaag agcaatccaa ctaaaaaggt cttcccttta ccggtttttc
     6361 cagttactgc tacatgaggc gcatcaaaca acgcaccttt gattccttcc gcaatagcta
     6421 gaagattcaa aaagacaaaa cgatttgacg cataaattaa ttcttctaat ggtgtttttt
     6481 ctcctcgaga accaccatcc cctaatgtat cttgccgacc aatataccag ccggattcca
     6541 tacctaattc tgatactgct gaaaacaacc attcgccaat tgcttctaca gttgttcggt
     6601 gcttccaatg caactgtcga cccaatggtt gccctaataa acatttatag aacaattctc
     6661 gttgatcatt ttttgcacgg tggatatgga tagtcgtatg aagctgttga ataatcgttg
     6721 cactattttg acgaacttct tcaaccgttt gaccgtatac aataaaacat gcgatccaat
     6781 cgacgacagg ttggccatta tctactcgtt gctttaaatg ttttactaat ccccgaacag
     6841 ttaataagcg ttcgctaggt tcttcatcgt tttgaaccaa ttccttatgt tctgttttga
     6901 accgttccga tagccaattt agttttaaac caatacctcc ctgtcctttc aagcgtggat
     6961 agtgaatttt cattcgtaat tctgctggga aagaaagatc ctgtgcaaat tgaaatagat
     7021 cacagtttct catattttca ggtgtttctc caataggtaa caacgctaaa taactagtcc
     7081 ccaactcatc ttctaattga agaaggcctt tatgttcctt cggcatacgt acatacgttc
     7141 cttgattgtc tttcagtact tcccaattat tttgttcttc tactttatga gccattcctc
     7201 gaatatattg cagacgattg agatacaaca gctctttttt tactaaggga cgaatagaaa
     7261 agctataaag taattgtgtc acttctttta cataaggatc actttcagaa aacaaagttt
     7321 ctaaatcatc tacttctaat ccaaataagc gtgctaaact tcctgctaca tctaaccaga
     7381 ttagatttag tacatcataa aaactctcca cggttgcttt ttctttgact ttgattccta
     7441 aaataaagga aggacgatag acttgacctt ctcgttccaa caatgtcact gtttcttttg
     7501 cataatacgc accaattgct tgactatcgg gagaaaatcc ctttcctaat gtgtcaaatt
     7561 tcgtccgtaa attcattgtt cgtggataca tcaccaactc cacatcttca taatctgcta
     7621 aaagtaacag taaacgagcc atcatttctt ttatttcctg tgtctttttt ggtgtgtttt
     7681 ttgaaatggt tcgtggaaaa acatgatagt aggcccagat acaatcctcg ccatttaaca
     7741 aacaattttt ctttaatgct ttcactggtt cttttacaag taccaataga tctcacctcc
     7801 caattaaaaa aaggaacaac cagttgattg tccctttttc taatcctcta aataaacata
     7861 cttattcgca aaaagtaatt cttccaatgc attatttgat accttgtccc agttataata
     7921 cacacaatca ccattcatca tacgcactac ataacaaaac caatcccata aataaaaatg
     7981 cacttttttt ccatcaggtt ttagttttgc cagtgctaac acacataaat agggtagccc
     8041 tataaaaaaa acaacccctg ctggtggaaa aaaagaaata acccaaccaa taaatgaacg
     8101 taaaaataaa tattcaaaga ttacgaccaa taaaaaagtt gctgcattga ctaatgcaac
     8161 tggaaatgga agacgccaat tgcttgtaat ctgttgtacg cgataaggct ccgcataaac
     8221 aatcgaataa tcgtaaggtt cccaatccac ttcactatcc tcctaatcca taaataattt
     8281 ttcaatgatt ttaccaaacg catcaaatac acgcgttgga tcatttaaga agaaatagac
     8341 tgcggcacct gctgcgatag atattaatgc atttcctact tttgatttca taaaatttcg
     8401 caatcccaga taaacaacca ccattattaa aaaatagcca ccttgcgtta atataaaatc
     8461 tacaattccc ttcagtgtca tttccgttcg tctcctttcc tatccaacca attatgtgtg
     8521 agtttttcaa cataatactg atttgttttt ttcgtaataa caagcgtcat ttgttctttt
     8581 tgtaaaagtg acgtatcacc atctcgaaag acgactgttg cttttacaat aaaagttttt
     8641 ttcgtttctt tataacgcaa tactttcatc tctacttgat caactttagc tgtctgattg
     8701 agtccttcag gttctttcat catgtatgac atatcttttg cattgttttt cgcatactta
     8761 tctaagaaat cttccacaaa tccttccaca tctttcgctt ccgacgcatc aaccgtttcg
     8821 tataatgtgt catcataagt aatcattcgt tgtttttctg tattctttgg tagtgccgta
     8881 aaataaggat aagcaataat acgacagccc ttgtttgaaa tagaaaaggg aagattgagt
     8941 acttgtgtga ttgtcacact tgtttctttc ttattctctg gatccatcga gcgaatatca
     9001 taagttactt tataactagc tgttggaatg gtttcaattt cttttaatcc aataaacgaa
     9061 gcagcagtta attttcgaat acctgaaaca cttcctggtt cttgaataaa gttttgtgca
     9121 taaaactctt tttttaatat ttcttgacgt tgttgttctt gttcatgatt atcgaatgta
     9181 ttcatataca aagcaacaaa acgattcata aaggattcaa tttcaggtgt gtacgttaat
     9241 tctgactgac tttgtacttc tttcttttgt aattgttgga tttcatcttt caaaaacgac
     9301 acttgaacct tcgttttcat ggattgaaaa aatgccaagc cccctgaaaa acaaagaaat
     9361 aaaaccaaac accaaatcca tttcactgct ttttttgaat ggattttcct ttgattcggt
     9421 actttctctg tataaacttt ttctttccgc cgtagttgtc gttttttcat aagctactcc
     9481 ttctagtatg tttttactag tacgtacgat tgacaacgat aagcttgttt ttctgaaaaa
     9541 tagtccattc aattaccctc attttctatt ccttttctct ttcttttata tccaacaaat
     9601 ccagcaatac ttgtcaacac tcctcctaga acagaaagta caacatgtat ttcttcacca
     9661 gtctttggta agacttttgt ttttccatct gctgttttga cttcaatatt gccacttgga
     9721 agtttcttta caggaatcga caaatccttt accaaattcc cttcactatc ttggattaat
     9781 ggaattccat cttttacacc aacaattgtt tgtccacctt ctgcttttag tggagttgtt
     9841 ggtgttggaa tatgggtcaa ttcctctaca ttactagtga caatcggtac actcattcct
     9901 tgagaattat tcgtcgtaat ttcaccgttt ggggttaccg atactgtgac ttcttttgct
     9961 ggaacaatag gtttattttc ttttggaatt gcttctgatt tttcagcagg ttgtttcggt
    10021 tcttcttttg gttcttcact ggtaaacgac atcgttggct gttccgtact tgacgaatcg
    10081 gtattcgttt cagaagaact ggttgttcct gattgttctg gagttgtgac tatacttgaa
    10141 tccgttgttg ttgaactgtg cacaccttct tcttcaattg gatcagtcgg caaagttgat
    10201 tcaggaatct ctggttctgg tgccactggc tctttttcag gtttctctgg ctgcaaatgt
    10261 tcaggttctg ctggtttttc cgttgttcct ccatttagct ctgtctcaac atctgataat
    10321 ccttcccctg gcaatgtttc tacaatttct gacttaggtg tttctacagt ttcatctgca
    10381 taaaccaatg aactccctaa ttgaaacgaa aataatgaaa ttcctagtgt tgttacgact
    10441 gctaatgttg tttttttcat gattattcct ccaatttttt atgcaattcg tgttttgttc
    10501 atagacgccg cactaatatt atctgaacga taaaagaact cccgtccgtt ttcttctaac
    10561 ccaacatatg gattattttt gaataattga atcaatagtt cactggtgtt gtattttgga
    10621 tagtttgcaa aaaaataata ctgtgcttgt cgcccatccg aaaattcaac aagtaattca
    10681 taacgatacc gcttcaatcc ttttcgttgt tttaaaggat ctctatacca acttgtccat
    10741 cgttccgttc gtggaccgag atagacacga tacacataga acaatgccat accacatacc
    10801 accaaaaata ataatcgcca cataaaaatt cccccttttc ttctaccttt cttgaatcac
    10861 cgaacattca taccctcggt agagatccga aaaatccatc attaattttt cgtataaacg
    10921 ttgttgcaaa tagcgattgt ttcgaataca taattcttca tagccatcaa tcacttgatc
    10981 ttctttctca tagcatcttg tagctctatg acgctcagct tgcatgacat aaccaaactc
    11041 tgcaccatat cgttctaccg ttaataactg ttgactacgc aataatgcaa tataacaatc
    11101 acaaaaatgt tcagccgaca tttgattttt ctcacgtttg tgaaataggt attctttaga
    11161 aagcacatta ttcgttaaac aatctgataa atagcgataa tctggcggtc ggttattttc
    11221 atctgtgaat ttttcccata ataaactgat ttttattcgt tcagcggcta gatagtcttg
    11281 ttcgcgtgga taaaattcat caatcgtaca aagctttttt atcacccgaa taaacaaatc
    11341 catataatgc gaaacgaact cttccttttc aattggctct cctaaaaatt ccagtgacat
    11401 accatcttct ccttccttaa aaaataaaaa agcagaaacc gatttggctt ctacttttct
    11461 ttccttttat agctcttttt tacggaagaa tccgcaaatg acattttttc aaaaaattaa
    11521 tcggcacaag ttcacgaaaa cttggagcaa ttttagatag aatcacttcc ccatcttgta
    11581 aaatacaatc ataaaaatca tcagttactt gatacttacg cttcttacaa taattgataa
    11641 aatagtagcg atcattttct atttccattc gttgttctac taagcgcata cgaacatcat
    11701 catagaaata agccacaaat cgcccttcaa gttcacgttt ttcaatggga tactcttttg
    11761 gtaacaatct attttttcgt cgtttataca ttgctggata ccttagaaga agttcatcca
    11821 aacgtctgtc agtatatggc aactgacgaa tcaatgattc caatcgttct ttatacagat
    11881 agaataattg gcgcaagcaa tagtgtactg cttctggatt ttctaatact gttggatcac
    11941 gcgaccaata cgcaccaaca taaatcataa tttttttcat ttagacaatc acaccagcaa
    12001 tactaatcac cgatccacaa taggtagaac aagtggcata gcggccgatt tcctctaatt
    12061 ccagctcaat ctcttctaaa gaaagatacg tctgttcttc tgttggctgg cgattcattt
    12121 cccaagtaac agttgccaac gagatttctt ccacaagagg tttttcgttg ctaagtgctg
    12181 ccacacagtt ttcgtatatc ttcattttat cgcagccttt ttttaccgct aaaatcgcag
    12241 gtaccggcaa tgaaaatcga taaaagcgaa tgaattttag cgttcctttt cttcggctaa
    12301 attccaactc ttcgagttct tgttcgtacc ccttacgaac ttctaataag cgtgcaattt
    12361 cttgccagtt ttctttttcc tgataacgtc cagcctttac cgcttgttca atataaggaa
    12421 ctcgaaaacg aaaataagac aaatcaacca tcttttcatt taatgcttca attgcagcct
    12481 ttaaatccaa aagacgcagc cattctgttg tcgtatatgc attcatgcaa ttttccctcc
    12541 tgacgttccc atccattcta gatagctttg ttgctcgtca tttagttcag cattctcaat
    12601 tccttcttgt acaaagtctc ttcctaaaat acgatctgct tcttgtaata actttaatgt
    12661 cggagctact tgtttcaaaa accagcgttc tgaacgatct aatgagaatt tttctggctt
    12721 tacggtaaaa tccaattctt ccatcgaacg aaacaatttt gcccatcgtt catcaacaat
    12781 tcgtgcatca ctttccagtc gatcccaaac aataagtttt gcattaatta aaccaacgcc
    12841 aatctctcct aaacgtttag gggaatgatc caaaataaac gcttctactg cttgtgttgc
    12901 tttttcatta gctaggcgta actcatatcg attataaatg ccatatttcg ttaatgcttc
    12961 tatttctgaa attttctcac gattcgcaat ttcaaaacgt ttttcgtaga aattgaaaaa
    13021 caatggactc tttcgcgatc caaaatacaa ggataaccct tcgcttcgga aataaccatc
    13081 atctaaaaaa caaccgccaa tattctgaaa tttcttaaag tccatttcta ataaacctgc
    13141 tttttgtttt tctagtaatt caaagagatc aaaatgttct tctgtttcca atccccaaag
    13201 ttcatctaac gcaatatcga atcgactaca ttgtactcgt ttataaccag cttccaactt
    13261 tccttgattt ttccaaatgg aatatagcca tgttcgccaa tctgttcgtt gttcacgcat
    13321 gacttcctca tattctcggc aaccttgacc cgaaaactgt acaagtacat tttgtgttac
    13381 agtttgtttt gaatgccaga atacccagat atttccacga acagaaagac aatcaaaaag
    13441 atacaagccc gtcggacgtg tttcaaattc tgttagatcc attcgtaaca cacgttgaat
    13501 cactattttg tagtctgtcc caaaaaagga gagacgcaaa tagtcgatca ttgctcgaag
    13561 atctattccg cttttcatat acaatcgaaa gcgttccttg attcgttttt cgaaatcctc
    13621 tgggattttc ttttttcctt gttccatctt ctttatataa tctaccgagc atcccaataa
    13681 ctgtgcaaaa cgtttttgag agagtaattt tttccctcca cctgaagcgt gaatagcact
    13741 caattttttt cgataatttc gcaattcaat aggtgtcata ttctacctcc tttataaaat
    13801 aacaaatttc gattttctta atctgctaga aatcccgata taacaggtgg taccccctaa
    13861 tttttctaaa aaggagtact ccttctaacc ttagctaaag ccttgatata actgatttaa
    13921 atacactttt ttatttgtgt tatgttattt ctgcccccca tattatatat cggggggctt
    13981 tacacaaaga ggccgtgcgc aagcgcacga cctctttgtg ggcacacatg ccaagcatgt
    14041 gctgccacga ctggtctcgg tggcttcgcc gacacaagcc gcggcagcac atactcggac
    14101 aatgtgttct cccatagtcc ctatgaaagc caccccgata tctaaaccaa tgaatcttct
    14161 ttttcttgta attctttata ctgttcagga gcaaaacact gtaaaatcgc taaacgtaac
    14221 tgtatgtttt tctctttgca taaccgatat acctgatgaa aacgcaatat atcctctttt
    14281 gtcaaaacaa tctgatttct cgtaacaaac aatgtctcgc gctcaattcg tcggaaaaca
    14341 taagaacctt ttgtgtagta ttctactttg ttaatccact cgtataatct tcgaaccgca
    14401 aaatcaggtg aaatttcacc cgcagctgaa gaaacacgat acaaaacttc acccatactt
    14461 caccacttcc ttctaaatta taaaagtgta taactttcct catctacccc atttgttttc
    14521 gtatttcctt ttaacgattg cgcttgacga tcaatttgtt cttcgtaact agtaaacgcc
    14581 tcttctttca gtacagaaaa atcttgcaca atcattgtgt caaatttttt aaattcctca
    14641 ataaagtgat agtctttgta aacaaatggc gcaaagaatt ctacaggcac atcaaaacca
    14701 ggaaaagcat acccacgacc aggttgatct ttgttataaa acggcttatt cttaaaatcc
    14761 tctccaaaag tcattaaata gccaatggga gataaacggc caacagaaat acggaacgtt
    14821 aaactatctc gaacaccact tggaaaatat tccgcatctg gtcgctgcat cgctaaaata
    14881 acatacatgc ctgcttgacg accttttaat actaatggct gaattaattt atcaactttg
    14941 tttctcgttt ccatatccaa cgaaccatag aaagctgccc attcatcaaa aaagataaaa
    15001 tgcggcggta tcccataata agcataattt ttccctggtt catacttagg ctctttcatc
    15061 gttttcatat aggtatatcg ctgattcatt tcttgtaacg catttcgtaa gcaaataatc
    15121 atttcttcat tcgttttgta aaacacatgt cctttgaata catcaatatc acctaatgcc
    15181 gctaaatctg ccgctttccc atcgcaaata tctactgtcc ctacatctaa acaagctttg
    15241 atcaatgaat acaataaata ggtttttccg ccaccaatcg caccagaaat taatagattt
    15301 ggcgattgat catataccca atccaatgtt ttcattaaat gaatacgtcc attttctgct
    15361 atacattgat caataggaat ccgaccagaa tcggaaagaa atacataatt gattcgggct
    15421 ttttctcgtt cttcacgaat taaatcacgc aaaaatactt cggataataa gctacccatt
    15481 tttaaaaatc gttcgtggaa acggccacca tctaaaggaa aagaaaccac taattcttgt
    15541 ttcttttgat aaaaataaac ttttggtaat ctcatttttt ccttcgtttg tttctcacca
    15601 acttgcttct ttgtataaac aaaccgattg tcaattaaca ttcgagcaag taactgacgc
    15661 caataaacag aagcaaaata tccttccttt agtcgcgacc aacgcatcaa acctaaaatt
    15721 aatccactaa ccagcaacaa atacatcaca atactgattc caggaataag atacatttgc
    15781 caattcgaat acgtatttaa acgaggataa aatactttcc aactaaacca acttaataga
    15841 atcaaaaatg gtaacagaaa gaaaccataa taacaaaacc ataaataacg attgaaataa
    15901 cgaattcgtc gaccacgata cttaaaaaaa tgaggttcca attgtcacat tcctcctttc
    15961 tgtttttact tctcctgtgc taaaaatttt tgacaaaaag caactaatac tggaatatca
    16021 catttaataa aaaaccataa tcgaaaaaga tttaatcgat ttttatatgg gtgtgcacac
    16081 atattacata ttcgtactac acgaatccaa accacttcct gatggtcttg tttccactga
    16141 tacggtaatg aaaaacgaac aagatcacgt aaaaacatac actgcccaat aaccgcacaa
    16201 atatactcat ttgtttgtat aaaaatcgtt ggattgtctc caaaatactc aatcaattgt
    16261 tggatatttt gacaacaaac cataatctct cgaagtgttt gattggtttt gtccagattc
    16321 aaaatctccc cttccttcta ccttaaatag aaagagtagg aagacttcct cctactcttt
    16381 aaaaaactta ttttttctct ttttccttag aatcagcact tggttgtttt tgatctaaac
    16441 ctttttcttg tgattgtttc ggattagaat tatttgttgg agcttctact ttctctaatc
    16501 ctttcgcata aatcactaaa tctgaatatt cacgattgcc atcttgtgct ggaatatata
    16561 ccacatgata ttctggatca attaatttca caggatcacg tcgtttaaaa ccagtcaaat
    16621 ctacatctgc tggaaattta actcgtcgtg caatctttgg ttcatctgga tttccaaaaa
    16681 ctaattgtgc tcgctgtgat cgaatttctc ccgtataatc gcctgtttca ttatcaaata
    16741 cacgatcgga ctcttctaat gcgccaaagc gtacggctcc tatacaagtt tcatctaatg
    16801 cttctgtaat atcataatta tttggaaatt ttaatcccat cttacaaaac ctcctcaatt
    16861 tttacaccag ttaaaaagac accgacatct tgatagccag ttgttcgacg ctctgtttgt
    16921 cccatatcat ttgtttcacg actattcact ctttctttac gaaaaacaaa ataagcttct
    16981 tcaaatgaaa tacgtacctc tttttctggt aatcgtaatc ttttatcctc ttcagctgtg
    17041 agataaacaa aaaaagcagg ctgttcttct cgcgaacatt ctgcttgtaa ttgaacatat
    17101 cgcccagaca caccattaat ttcctttggt ggattaggtt cccgtgaacc caataaaaat
    17161 acctgaccta aaaactcttt cttcatacgc aatgcttttt ctgccatatc atttttcctc
    17221 ctcttaaaat tcacaatcaa ttctaaacga ctctatatta cacaatggaa agtaatgttt
    17281 aacaacaaat ggctcatcac taggagacat atctactatt aacatcccgt ctcgttcatg
    17341 cacagaaata tcagtacact cctcatacgt acattctttt ccactcaaaa aacaaatatt
    17401 tatatttttc ttttccatat tcttttttcc tccattattt tcttttctgt aaaaaccaag
    17461 taataattcc ttttccatct tccaacacaa actgatgata tatttcccta gcaagaatcc
    17521 acacacatgc taaaataaag aaaataataa tactactaaa gatgcactca tccttgctgt
    17581 caaacggaaa aagatatatc ataattgcct cccccataat taagaaacca attgatacaa
    17641 acaaaaactc taccatcata caaaagaata tttcttccaa ctcttttaca tcctttctaa
    17701 ctttttataa aaaaggagat tatttttata aacactttta ttcaacaact attttcaaat
    17761 gaattacaca ttatcatctc aagtatagtt tcaatcgtca ctattgtttg gtccatttct
    17821 agcttttatt taaaacatcg ctatacaaga aaaaagaaaa gcactgaaac cctaacaaaa
    17881 gaaatgttta ttccatt
//
LOCUS       DACEMO010000038        17335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEMO010000038 DACEMO010000000
VERSION     DACEMO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.038"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(154..849)
                     /locus_tag="IW728_000988"
     CDS             complement(154..849)
                     /locus_tag="IW728_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1024362.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(939..2039)
                     /gene="ychF"
                     /locus_tag="IW728_000989"
     CDS             complement(939..2039)
                     /gene="ychF"
                     /locus_tag="IW728_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1024363.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2048..2239)
                     /locus_tag="IW728_000990"
     CDS             complement(2048..2239)
                     /locus_tag="IW728_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1024364.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2260..3150)
                     /locus_tag="IW728_000991"
     CDS             complement(2260..3150)
                     /locus_tag="IW728_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1024365.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3137..3904)
                     /locus_tag="IW728_000992"
     CDS             complement(3137..3904)
                     /locus_tag="IW728_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1024366.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3916..4629)
                     /gene="rsmG"
                     /locus_tag="IW728_000993"
     CDS             complement(3916..4629)
                     /gene="rsmG"
                     /locus_tag="IW728_000993"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1024367.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4861..6762)
                     /gene="mnmG"
                     /locus_tag="IW728_000994"
     CDS             complement(4861..6762)
                     /gene="mnmG"
                     /locus_tag="IW728_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1024368.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6779..8176)
                     /gene="mnmE"
                     /locus_tag="IW728_000995"
     CDS             complement(6779..8176)
                     /gene="mnmE"
                     /locus_tag="IW728_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1024369.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8520..8858
                     /locus_tag="IW728_000996"
     CDS             8520..8858
                     /locus_tag="IW728_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024370.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9026..9784)
                     /locus_tag="IW728_000997"
     CDS             complement(9026..9784)
                     /locus_tag="IW728_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1024371.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9802..10620)
                     /locus_tag="IW728_000998"
     CDS             complement(9802..10620)
                     /locus_tag="IW728_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1024372.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10617..10973)
                     /gene="rnpA"
                     /locus_tag="IW728_000999"
     CDS             complement(10617..10973)
                     /gene="rnpA"
                     /locus_tag="IW728_000999"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1024373.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11091..11225)
                     /gene="rpmH"
                     /locus_tag="IW728_001000"
     CDS             complement(11091..11225)
                     /gene="rpmH"
                     /locus_tag="IW728_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1024374.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11748..13082
                     /gene="dnaA"
                     /locus_tag="IW728_001001"
     CDS             11748..13082
                     /gene="dnaA"
                     /locus_tag="IW728_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1024375.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13280..14410
                     /gene="dnaN"
                     /locus_tag="IW728_001002"
     CDS             13280..14410
                     /gene="dnaN"
                     /locus_tag="IW728_001002"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1024376.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14639..14881
                     /gene="yaaA"
                     /locus_tag="IW728_001003"
     CDS             14639..14881
                     /gene="yaaA"
                     /locus_tag="IW728_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1024377.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14868..15992
                     /gene="recF"
                     /locus_tag="IW728_001004"
     CDS             14868..15992
                     /gene="recF"
                     /locus_tag="IW728_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1024378.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15989..>17335
                     /locus_tag="IW728_001005"
     CDS             15989..>17335
                     /locus_tag="IW728_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1024379.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNRE"
BASE COUNT         5002 a         3554 c         3222 g         5557 t
ORIGIN      
        1 cgagaaactg gcttttgaac accgtttatc gtaaaaaggg actgtgacaa gacttttgtc
       61 acagtccctt actattattt aactatggat aaacaaaatg aggttcttta ctacccctag
      121 atatctagca aaatagctga tctttttgct agtttatctt cttgggacgg ataaacttcc
      181 aataatcccg tattttttct tcaagtagta acggattgct aaagcagttg ccccgataat
      241 gataactact actggatcta atactggatt gataactggt ggcaatagtg ttgtcgcaga
      301 aaacagtacg acccaaagaa gtgtcgttcc acctaggata agcaatgtct tacccattcc
      361 tggtttcttg cttcgatctg ctccgggata ttcatattga tagatgtatt tgtacatcat
      421 ataaaagacc cagccaccga ttgcagaacc tagaacgagt gtagaaaagc cataagctgg
      481 cgttgttcca cgtgaaaaca atccggtaat cgcaatcatg atacttaata gaccaagcaa
      541 cagcaacgta ttatctaacc acatcaataa aggggtggat gaatttttta cttctgctgg
      601 cttattgatg attgcttccg ttctttcaga gactgtccca aacaattgac gggctgttct
      661 tccgcctttt tgttctgcaa ctaaagtagg cagaatgtca tgaagggctt ttactttttc
      721 ttcttcagac aaattcgctg cttctaaaga ttttttcaaa tcaaaaatgt actgttgatt
      781 tcttttggtt aattttgttt ctaaactatt gttttctttt acgattgctc gtaattgttc
      841 tggttccatt atctaatagt cctccttagc cgttcctttt ctcaacggtc gtcacaaaag
      901 acattctacc atattctaca gctagtttga tagccctttt acacattaaa gcggaataac
      961 attacgtcgc cgtcttgtac gacataatct tttccttcta atcgaacacg tccagcttct
     1021 tttgctgcat gcatgtttcc gtaatgatcc aagtcttcaa aagagacagt ttctgcacgg
     1081 ataaatccgc gttcgaaatc tgaatggatg attcctgctg cttgtggtgc tttgattcct
     1141 tttttgaatg tccaagcacg tacttcttgt tccccagcag taaagtaagt agctaagcct
     1201 aacaaatcat aagcagcacg gatcaactga tccaaacctg attcttcgat tcctaaagct
     1261 tctaaaaatt ccgctttgtc ttcttcatct aattctgcga tttcttcttc tgcacgagca
     1321 ctgaccacga tcacttctgc attttcttct gcagcaaagt tgcgcacttc ttgtacataa
     1381 tgattcgcgt ctgcatctgc tacgtcatct tctgagacat tggctacata aagaattggt
     1441 tttgtcgtca ataaaaatag tgatttaacg attttttgtt cttcttcagt aaattcgatc
     1501 gttcgggcag attttccttc ttccagtaca ggtttgattt tatccagtac agctagttct
     1561 gccactgcat ctttatcttt ggttttagca acttttgcta cacgagtata gcgtttgttg
     1621 atagattcta agtctgctaa cactagttcc aagttgatcg tatcaatatc ttctagtgga
     1681 tcaacacgtc cttctacgtg agtaatatta tcatcatcaa aacagcgaac aacgtgacaa
     1741 atagcgtcta cttggcggat atggcttaag aattgatttc ccaaaccttc tcctttactt
     1801 gcgcctttta cgattccagc aatatctgta aattcaaaag tcgtcggaac agtttttttc
     1861 ggatgtacaa gttctgttaa gcgttgcaaa cgccagtcag gtacttctac catccctacg
     1921 ttggggtcga tcgtcgcaaa gggatagttt gctgcttctg ctcctgcttt tgtgatcgca
     1981 ttaaacaaag ttgattttcc tacgttgggc aacccaacaa ttccagcagt caatgccata
     2041 aatttattca ctctttctct gcattttttt caattacttt ttttaatttc ttttcaaaat
     2101 ctcgacgggt catcatgacg atatgtccgc aattcataca tctgattttg atatctgcgc
     2161 ccattcgaat gacttcccag cgattcgtct ggcaagcgtg aggttttttc atttcaacga
     2221 tatcgtgcaa gtcgtacata aaaatagcct cctttttgtt tattcttcat caaattgaat
     2281 attcaagata tcaagaattc gagtcaaatc tttttgagaa agatattcaa tctcgatctt
     2341 acctttccca tttttttctt ggatagctac acttgtacca aatttatcca tcaaacgatc
     2401 ttcgctttct cggatataat atggtttttc ctttgctagc cttgggattt ttttcttttc
     2461 ttttccatca ttttgattta actcagaaac aagctgttcc agttggcgga cagttaaatt
     2521 ttcagttact gcacgattgg ctaatttcaa gatcaatgtt ttatttttta aacctaacag
     2581 cgtacgggct tgtcccatag ataaacgctg atcttgtacc atttccttga caagatccgg
     2641 taaagaaagc aagcgcaaat aattggcgat ataaggtctg ctctttccta aacgactagc
     2701 tacttctgct tgtgttagtt tcaggttttt catcagcatc tcatatgctt ctgcttcttc
     2761 taacggattc agatcttctc gctgcaagtt ttccagcact gctacttgca tcatcgcttc
     2821 ttcgtcaaag tctctaacga tagcagggat cgtttctttt tcagctagtt tggacgctcg
     2881 aaaacgtcgc tcccctgcaa taatctcata tccttttact gcggaacggc gtacgatgat
     2941 gggttgaaag acacctgatt gttcaataga attagctagt tcctgcaaag atacttcttc
     3001 aaatgtttta cggggctgat acggatttgg tcgcagctca ttcaatggta gctggacaac
     3061 ggtatcatct tttacatcca ccgtctctaa gttttctaaa tcttgaaaca gtgcatcgat
     3121 tcctcggcct aatcctttac ttttcttcac gacttaccac ttcctttgcc aatgcttggt
     3181 agacttcagc tcctcgagaa cgtggatcgt agtcgatgat cggttttcca tgactaggtg
     3241 cttctgataa acgaatattt cttggaatga tcgtttcgta aactttttcg cggaagtatt
     3301 tacgtacttc ttctacgact tcatttccca gatttgttcg tgcatcatac attgtcagta
     3361 agactccctc gatctctaat tctggattaa agtgtttttg gactaaacga accgtattca
     3421 gtaattggct taatccttcc aatgcgtaat actcacactg aacaggtatc aaaatcgaat
     3481 cacttgctgt aaatgaattg attgtcagat ggccaagaga aggcgggcaa tcgatcaaaa
     3541 tataatcata ctgactgctt acttctgcta aagatccttt taatcttgat tctcttgcca
     3601 tcattgacgt caattcgatc tctgctcctg ctaattggag agtcgcaggc acaatactca
     3661 aattttcgtg ttcagtgatc aaagttgcct catcaattgg caattcattg actagaacat
     3721 catagatgtc tcttgtcaca tcaggttttc tgatcccgac accactggta gcatttcctt
     3781 gtgcatccat atccactagc aatacttttt tgccaaggct agcaagacaa gcacctagat
     3841 tcaccgtcgt cgttgttttg ccgacgccac ctttttggtt cgcaacagat attatttgtg
     3901 ccatctcttc tcctcctatt tgattggctg cttattaggc agtccgggct ttcttgggta
     3961 tttctttggg gttggtttct ttttttgaat aacgatgata tgccgttgat cagagacatt
     4021 cggcaaggta accgcctctt ctttgaggaa tttaccacct aataaagcaa tcgcttgttt
     4081 tgcttctgtc aattcctctt cactttttgc tgctttcaat gcataaaaat agccttcttg
     4141 tttagccaag ggcaaacaca attccgctaa cacattcaaa cgagcaaccg cacgagcagt
     4201 gacaaggtca aatgatgcac gaaagtgagg attttgccca aatgtttctg cccggtcatg
     4261 atataaggaa gcatcgattc ctaataatcg ggttaactca gataagaagg ttattcgttt
     4321 attcagagaa tcaacgatcg ttatctgcag ttctggaaag gcaattttta aaggtatgct
     4381 aggaaaccct gcccccgaac caacatcgca taaagataaa ggttgcgtaa agtctacttc
     4441 aaatgctaat gtaagtgaat cgtaaaaatg cttcagatat acgtcttttt catccgtaat
     4501 agctgtaagg ttcatctttt cgttccattc gactaatgtc cgataataaa tagcgaattg
     4561 attcatttga tgatcggtaa gctctatccc tttttctgcc aaggattggc gaaattcttc
     4621 tggtatcata aatattcctt tcattgtgaa acagcaattg tttcacagat tctctcccta
     4681 ctttaacgca aaatctctag acatgcaatc cttccatatg gattcttaaa aatttgaaaa
     4741 gtaagaaaag ccgatagact ttttctttct agtatgactc ataaaatata cacagaagag
     4801 agaactagct ttgagcaatt tctgcaaaaa ctagttctct cttctattta ttattacgta
     4861 ttacgctggt tcactgactt tcgcaatctt cccttgttcg atatagacca ttaggatact
     4921 taggtctgca gggtttacgc cactgattcg gctagcttgg gcaattgttt ctggttggat
     4981 tttctgcaat ttttgttttg cttctgttgc taatccgttg attgctgcgt agtcgatgtt
     5041 ttctggaatc tttttcgctt ccatgcgttt taatttttct actttttcca tggctttttt
     5101 gatgtagcct tcatatttga tttggatttc tacttgttcg atttcttttc gtgtcaattc
     5161 ttcattttct ggaataaacc gtagaagttc tgcataagta atctcaggac gtcttaaaaa
     5221 gtcgcttgct agtacaccat cttttaatgg tgccgcatta atcgttgcta aaaagtcttg
     5281 gacttcttct gttggtttga ttcgtacatt catcaaacgt ttgatttctt gttcaactgc
     5341 ttcttttttc ttcaaataat tcttgtattg atcttctttt actaagccta cacggtgacc
     5401 catttctgtt aatcgcaaat ctgcattatc atgacgtaaa agcagacggt attcagcacg
     5461 tgaagtcagc aaacgataag gttcgttcgt tccttttgtt accaaatcat cgatcatgac
     5521 accgatgtaa ccgtcacttc gtttgatcac taaaggttct tttccctgaa ctttcaaagc
     5581 tgcattgata ccggcaatca gcccttgtcc agccgcttct tcataaccgc ttgtaccatt
     5641 tgtttgtcca gctgtataaa gattttcaac gactttagtt tctaatgtcg gacggagctg
     5701 gtgtggctct actacgtcat attcgatagc atatcctgta cgcatcattt ctactttttc
     5761 taatccagca atagaatgaa gcatgttgac ttgaacatcc tctggtaaag aagtagataa
     5821 accttgaaca tagacttctt ccgtatttaa gccttctggt tccaaaaata attgatgacg
     5881 tggtttgtct gcaaaacgca cgatcttatc ttcgatggat ggacaatagc gagcgcctac
     5941 cccttcaacg atccctgtaa acattggggc acgatgtaag ttgtctcgaa taatttggtg
     6001 agtcgtctca cctgtatagg tcagccagca agattcctga tctagtttat aggcactatc
     6061 tggtgtgctg aagctaaaat gattcggctc tttgtcacca ggctgctctt ctgtaacaga
     6121 atagtcgatc gtacttgatt ttactcgagg cggtgttcct gttttgaacc gttcgatagc
     6181 aaagccaagt tcttttagat gattcgctag tccaatagaa ggttgagagt tattcggacc
     6241 tgatgaatat ttcaattcac cgataatgat ttctccgcga agtgctgttc cggctgtaat
     6301 aacaactgct ttggcccggt agctcgctcc tgtggacgtc actactccat gacaaacacc
     6361 atcttcgacg atcagttctt cgacgattcc ttgtcttagc gtcaaatttg gttctttttc
     6421 gatcgtatgt ttcatttctg ttgaataagc atgtttgtct gcttgtgctc gcaatgctcg
     6481 gacagctggg ccttttcctg tgttcaacat acgcatttgg atataggttt tatcgatatt
     6541 cttacccatt tctccgccta acgcatcgat ttctcgaacg acaacgcctt tagctggtcc
     6601 gcctacagaa ggattacatg gcatgaatgc taccatgtcc aaatttattg tcagtaataa
     6661 tgtttttgat cccatacgtg cagcagcaag tgcagcttct gaaccggcgt gtcctgcgcc
     6721 aacaacgatc acgtcatacg catctgcttc ataatgttcc aatcgtttgt tcctcctttt
     6781 attttcctaa acaaaattga ctgaataatt gagtgattag ctcatcttgt acactgtcgc
     6841 cgacaacttc tcctagatag tcccagcaac gcgtcatatc tatttgaacg agatccacag
     6901 gcattcctga atcaatacct gcaatgactt cttcaagtga gagggaagct ttttctaaca
     6961 acgcgatatg gcgggtatta gaaacataag tagcgtctct ttctcctgtt tctccgctga
     7021 agaataaatc agaaatagct gattccaaac gatctagtcc gtcgttttta gctacggaaa
     7081 ttgaaaaaac tggttcattt tcgatcaatt tcttcaattt ttcttgttcc agctgtgctg
     7141 gcaaatctgt tttatttaga agaatgatcc gtttcaatcc acttgtcgct tccaataatt
     7201 gttcatcttc tgctgtcagt ggttcgcttt ggtttagaac aagtaagatc aagtctgctt
     7261 ctgctaatgc ttttcggctt ctttcgaccc cgatccgttc caccacatct tctgtttccc
     7321 gaattccagc tgtatcgatt agcttgagcg gtaccccccg aacattgaca tactcttcga
     7381 ttacatctcg agtcgttcct gcaatatccg taacgattgc tttttcttct cttaacaaat
     7441 ggtttaacaa acttgatttg ccgacatttg gccggccgat gattgccgta cttaaccctt
     7501 cccgcagaac cttcccttgc ttggatgtag caagtagcgc ttggattctt tgttggatca
     7561 tctgcgcttt ttcaagcaat aacttcgtag tcaattcttc gacatcatca tattctggat
     7621 aatcaatatt tacttctact tgtgctaatg tctctaaaat ctcttgtctc aatgagcgga
     7681 tcaaagcaga cagattgccg tctaactgat taagagctag tcccattgct ttgtctgttt
     7741 ttgcccgaat caaatccatc acagcttctg cctgtgaaag gtcaacacga ccattcaaga
     7801 atgctcgttt tgtgaattcg cctggctcag ctaatcttgc accttctctt aacaatagct
     7861 gcaaaatttg attgactaca acgatcccac cgtgacaatt gatttctact acatcttccc
     7921 gcgtaaatgt ctttggtgct ctcatcacgg agaccatgac ttcatcgaca agctgattgc
     7981 tcttgggatc aacgatatgt ccataatgga tcgtatgact tggtacttct gataatcgtt
     8041 tgtttccaga ttgataaact ttatctgcta gttgcacagc ttgatcgcca cttaaacgaa
     8101 cgatactgat tgcaccttct ccaggtgggg tagaaattgc cgcaatcgta tcaaattcta
     8161 aagtaatttc agccattttt tcacttcctt tttcattttg cgcacaaaaa aagtgcccac
     8221 tccacccttt atgcacaata aacggtgaac ttcgcacttt gactgcttgt tcttgattaa
     8281 attctggtca tagattatca cacttgctat aaggaatcaa gtgtttaaaa caattagatg
     8341 aagttgagcc tgttctgctt ccattacctt atatttaatt gtccttggat gtctctttgc
     8401 gttctttggt ttattcgaag agaagatact gtataaacaa ggctttatct ttcttaaaaa
     8461 aaacggtaca ctaaatggta gcgctatcta aactatacac ttttgaaagg agaaaatatg
     8521 tgagcccgtt cagatctgct gcattggaag aagaaagtga ccctcggatc aacgatgtcg
     8581 ttttatctac agggaatatc aaccagaaat atatcgtccg cgatatcgtt tttgttgtgg
     8641 aatgcttaga agctgatttg tttgacccat cttttcccaa agatgacctt ttcttaacaa
     8701 cgaagaggaa attaaagacg aaggtacttg aatatggtgc gaatgcagta atcaactgtc
     8761 attttgagta tcagcaaaaa aaacgcgacg gaaagtattt tctggaactt tttgcttatg
     8821 gaacagtgat ccaatttatc caaacaacga ttggttaaat gaaacagaca aaaaaagcgg
     8881 atcaaaactg aagacagttt cgatccgctt ttttctatca aagttagtga ctacttcatc
     8941 gtatactctg ataagaatga tttctagtaa aatatacttg gtttgaaaaa ctttgtcaac
     9001 gtgatttatt caaaaagtaa tggaatcaat aatatttttt agcaggttct actactaaat
     9061 aacgataagg ttcctcacct tccgaatgtg tttttacatg ttcatctttg cttaatgcag
     9121 catggatttg tttacgttca aatgcaggca ttggttctaa gaaaacaggc tgccctgtcc
     9181 gttttacttt ttcagccgtg cgatcagcta aacgctgaat gatctcttgg cgcttttcac
     9241 ggtaattgcc tacattgact actacagaca gcttatttct tgctaaacga tggatgaaga
     9301 cttgtgccag atattgcaat gcattcagtg tttttccatg tttcccaatc agtatgcctt
     9361 gcttttgtgt ttctaaatgg aaaacaacca tattatttga ttctctttcc attttgacaa
     9421 gagctggagc atcaagctgt tttgaaattt cagtaagata aactgccaat tccgttaaag
     9481 ctgcttcatc acctagctca cttacttctt ctgttttttc ttcaagaatc gtttcgttga
     9541 ctacctcgat ctcagctgtt ggttcttctt ccaaaatttc ttctacagtc tgttctactg
     9601 tttcagaaac tttttcgctg atacttggct ggatcgaaag ccgtgcattc tttcgaccca
     9661 ttcctaggaa accttttttt ccttcttcca gtatttcgat ttcgacttgg tctttggcca
     9721 aacccaaatc agccagacct agttggacag cttcgtctat tgttgcagct tcataaatcg
     9781 gcattagcat tacctcccct attatttccg ttttttctta ggattcattg ctttcttaag
     9841 agcacgttct ctttcgcgga ttctccgtgc ttcttcttca cgctcttttc gaattttgaa
     9901 agggttgttg atcagcagcg tttgtacaac ttggaaggca ttggaaacta cccagtataa
     9961 tgaaagaccg ctggctaaat tgacacccat cactaaaatc attacaggca tcacgaaatt
    10021 catgatcttc attgatgcat tcgattcaat ttgactcatg ctagataaat acgtacttgc
    10081 aaaagtaaag attgctgcta aaatcggcaa aataaatgtt ggatccgcgt tccctaagtt
    10141 caaccataag aaatgccctt gtttcaactc tggtacacgt gaaatagatt gccataacgc
    10201 catcaagatt ggcatttgta ctaataaagg aagacaacca gcatatggat tgacgccatt
    10261 ttctgcatat aaacgctgct gctcttctct taacttactt tgtgtttccg gatctcttga
    10321 actatattgt tgctgcagtg cttttaattt cggttgtaat tcttgcgttt tacgcatgct
    10381 ctttgtttgg aaatgcatca acggcaatag gatcacgcgg atgatcagtg taaataaaat
    10441 gatccctatc cctgcattac caaatgacaa cgccttgatc gcctcagcaa agtaataaac
    10501 aatataacga tcccaaattc ctgtactctg tgcgctgact tccccagttc cgcaaccgga
    10561 taagacgatg accaaactaa cgataccggc catcaaaagt aaacgtttgt atttcttcac
    10621 ttactctttt cctctctgtc gattattttt gcaagtttta agacgtggac taaattggag
    10681 cgaacctcat cataagtcaa ccccttcaca cttggtctag caatcacaat aaaatccaac
    10741 tccggatcaa tttcttcttt tatagaataa actgctgctc ggatttttcg tttgacttca
    10801 ttgcggttta ccgcgttgcc gacctttttt cctacagata aaccaactcg aaaatgaggc
    10861 tgtcccggtt tttccaaacg gtaaacgacg aatttgcggt tagcaaaaga atctcccgta
    10921 tggaacacgg cttgaaactc tcgttctttt tttactcgat acgcttttct cattagtttt
    10981 ctttcctttt ctatctctca cttacgctca taatcatacc ataattacga ttgtcctttc
    11041 atcatttccc taaaaaagag tataaaaaaa accactgaaa ccccagtggc ctaagctgaa
    11101 agaacttttc ttcctttgcg acgacggcta gctaacacac gacgtccatt tttagtactc
    11161 atacgtttac ggaatccgtg aactttttga cgtttacgtt tatttggttg gtatgttctt
    11221 ttcatatatt ccacctccaa aatgttctta tctattttat aaacacagac atactttgat
    11281 agtataacga caaagtagtg attttgtcaa tataaaatag aagatcgatt gttaaaactt
    11341 atccacaaga ttattttttt ctatgggaat cctatgtgga aaaagttatg cacagggatt
    11401 atccacagat aataaaaggc tggttttaca cagaaaacac acgtgtaaaa aaagtaatcc
    11461 acaatcaggc gatttcctgt gtattccgtc tcctaactat tggtatatct gtatttttta
    11521 tccacagacg caagaggaaa tctattggtt tttacgagtt ttccacctgt gcattagtgg
    11581 tgtggaaaac tttttttgac aactgtgttt ttctcttttt tctttttgtc gattgtgtgg
    11641 ataattgtaa ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg aaaaaaatgc
    11701 taaattagta ctagttattc actttttaat gaaggaggag gccttatatg gtatccctcg
    11761 atgctttatg gaatgaatta aaagcaacat accaaaagga tttatcgcct gctagctata
    11821 atacatggat cgaaacagcg aaccctcgaa cccttgatca aaaccagctc gttgttgagg
    11881 ttcctagcaa gattcataaa gaatattggg aaaaaaacct ggcgacaaaa atcgttgaag
    11941 tcggttatat gttatcagga aatgagatta tccctcgctt tattactggc gaagaagcag
    12001 agcaagaaga agttatagaa gaaaaaaatc caaaagttgt ggcacctagt ccgctaaaaa
    12061 aagccatgct gaaccctaaa tatacctttg atacgtttgt cattggtaaa ggaaaccaga
    12121 tggctcatgc agcagcgctc gttgtagcag aagatccagg ttctatttat aacccgctct
    12181 tcttttatgg aggcgtggga cttggtaaga ctcacttgat gcatgcaatt ggtcatcaaa
    12241 tgctgcaaag tcagcctaat gccaaagtaa aatatgtaag cagtgaaact tttgctaatg
    12301 atttcatcaa ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa tatcgaaatg
    12361 ttgatttact gctagtggat gacattcaat tttttgcaga aaaagaagct acgcaagaag
    12421 agtttttcca tactttcaat gccttgtaca atgaagggaa acaaatcgta ctaaccagtg
    12481 atcggttgcc aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc tttgcatggg
    12541 ggctgtctgt tgatattaca ccgccggatc ttgaaacaag aacagcgatt ttacgtaaaa
    12601 aagcggcggc tgaacgtcta gagatcccag atgacacact aagctacatc gcaggacaaa
    12661 ttgattctaa cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct tttgctgcga
    12721 tgaatagtga agatatttcg actagtctag ctgcagacgc actgaaaacg ttaaaatcag
    12781 gaaaaggaca tcctcaattg tcaatcctgc agatccaaga agaagtggca aagtattatc
    12841 acatccaatt gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt gttcctagac
    12901 aaatcgctat gtatttggca cgtgagctca cagataattc tttaccgaaa atcggagctg
    12961 aattcggtgg caaggatcat acaacggtta tccatgctca tgaaaaaatc cagcagctga
    13021 tggattctag tgtcagtatg caaaatgaag tatcagaaat caaaaacttt ttactaaatt
    13081 aatttgtcga ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa cgttcaaaag
    13141 ttatacacac tttttccaca gctgtttttt cgtttctctc ttacgttttt tgagttttcc
    13201 acagaatcaa catgccctat tacttttatt attcttttta actaataata aataaacaaa
    13261 cattcaaagg agatacatca tgaaagttac tttaaaccga gctagcttta tgcaggaatt
    13321 gcaaactgtt caacgagcta tttcaagcaa aaccacgatc cctattttga caggtgtaaa
    13381 aatcacactg acacaagaag gtttgacttt gacggggagc aacgctgata tatcaattga
    13441 aacttttttg tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta ctggttccat
    13501 tgttttacaa gcacgtttct ttagcgaaat cattcggaga cttcctgaag aaacatttac
    13561 tttagaagtt ttagaaaata aacaagtagc gatcacttct ggaaaagcga attttatcgt
    13621 aaatggatta gatgcagata actatcctca tcttcctgtt gtcgaaagcc ataaccagat
    13681 gaaattacct gtacacgtat tgactaaact aatcaacgaa acagtttttg ctgtctcaca
    13741 acatgagagt cgtccaatct tgacaggtgt gcatttcatt ttatctgata attctttatt
    13801 agctgtagct actgattctc accgtctaag tcaacgcgtg attccagtag aacaagcggc
    13861 tgatcatttt gatattgtta ttcctggaaa aagtttgatc gaattatctc gctcattaac
    13921 aaatgaagaa gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt tcaaaacaga
    13981 aacgatgtat ttctattccc gtttgctaga aggaaactat ccagatacca atcgtttgat
    14041 tccttcaagt tttaatacag aggttgaatt ttctgttcct agctttttag cggcgatcga
    14101 acgggcttct ttactttctc atgaaggccg taacaacatc gttcgtttat ctattcgtcc
    14161 agatgcggtt gttttatacg gaaattcgcc agaaatcggg aaagtagaag aaagcttgag
    14221 ttatacagca agcagtggcg atccattaga tatttctttc aatccggatt atatgaaagc
    14281 agcgttgcgt gcatttggag atatgagcat caaagtgaaa tttatctctg ctattcgtcc
    14341 atttacattg gaaccaacgg aagatggcgt tcaatttatt cagctgatta ctcctgtacg
    14401 tacaaattaa caatgatttt caatgttctt gaaaatttaa ttgaaaagaa atagaaaaaa
    14461 taaaatttta aacagaaggc cctaaaatct cttttaaggc tttctgttta tttttgttta
    14521 aatcaccttt tttagattaa atcgtcttat atggaaaaat atatggataa atttgtttta
    14581 ttgcgaaaaa aaaggtataa tagagtatac ttttaataat gaaaatgagg gatgggcatt
    14641 gaaacaacaa gtcattctaa atacggaatt catgacgttg ggccaaatgt tgaaagaagt
    14701 cacagttatc ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca ctgtgttagt
    14761 cgatggcgaa ccagaaaatc gtcgtggacg caaactttat cccggcatga tggttgaagt
    14821 tccagaagta ggaacttttt ttatggtaaa aaaaggaaac gacgataatg aggctgaata
    14881 agctgtattt aaaaaattat cgaaattacg aagagctgaa catcgaattt tccaagaacc
    14941 tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa agtatctacg
    15001 tattagctat gacgcgaagt catcggacaa gtagtgagca ggaattgatt ggctggactg
    15061 acgatcaggc gatgattcaa ggagagatca caaaaggttc atccactctt ccattagaaa
    15121 tcttattatc taaaaaaggc cgaaaaacca agatcaacca tatcgaacag aaaaagttaa
    15181 gtacttacgt gggacaaatg aatgtcattt tatttgcacc agaagatctt tctttagtaa
    15241 aaggcagccc gcagaccagg cggaagtttt tagatatgga attaggccag atcaatccaa
    15301 tctatcttta tgatcttgtc caataccaag caattttgaa acaaagaaac cagtacttga
    15361 agcaattgaa tgaaaaaaaa caaactgatt tgctttattt ggatattttg tccgaacaac
    15421 tagcagcgtt tggcagcaaa gtattgaaag caagagcaca gttcgtcaag cgattggagt
    15481 tttgggctaa ttctttgcat cagcaaatca cccatcaaaa agagcagctg gaaatcgagt
    15541 atctcaccgc agtcgattct ttagaaacac atacgcaaga gcaaatccaa gaacaatttc
    15601 tcgcactttt gaaccaaaat aaaaagaaag acctttttcg gggaacaacg acagttggtc
    15661 ctcatcgtga cgatctttcc ttttttatta atcagaaaaa tgtgcagact tatggatcgc
    15721 aaggacagca aagaacaact gcactaagtg tcaaattagc agaaatcgat ttgattaaag
    15781 aagagacagg ggaatatcca atccttttgt tagatgatgt gatgagcgag ctggatgata
    15841 atcgtcaact ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc ttaacaacaa
    15901 cgacacttga ccatgtaaaa aataaaatga ctgttgaacc tgagattttt tatgttcaac
    15961 aagggaaact aaaaggagaa gaaactgaat gacagaagaa agaagtttag tagaacgcgc
    16021 taaagagtat gatgccagtc agattcaagt acttgaagga cttgaagccg ttcgtaaacg
    16081 acctggcatg tatatcggtt cgactagttc tgagggtctg catcaccttg tttgggaaat
    16141 tgtcgataat tcgatcgatg aagtattagc aggttttgca acgaaaatcc atgtgattat
    16201 tgaaaaagac aacagtatca ccgttattga cgacggtcgg gggatacctg tagatatcca
    16261 ggcaaaaact ggacgtcctg cagttgaaac ggtctttact gttttacatg ctggaggaaa
    16321 attcggcggt ggtgggtaca aagtttctgg tggacttcac ggagtaggat cttctgttgt
    16381 taacgcgctt tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg tgtactatca
    16441 agaatatcgt cgtggagctg tagtggacga cttaaagatt atcgaacaaa cggatcgcca
    16501 tggaacaacg gttcacttta cgccagaccc tgaaattttt acagagacaa ctgagtttga
    16561 tttttcaaaa ttggctaccc ggatccgtga acttgctttc ttgaaccgcg ggatgcgtat
    16621 atcgattgaa gacaaacgag aagaagaacc agtcatcaat gaataccatt atgacggcgg
    16681 gattaaaagt tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc cagaacctgt
    16741 atatttggaa ggtgaacagc aagatatcgc agttgaagtc tctatgcaat atacagacgg
    16801 ctaccattcc aatatcatga gttttgccaa caatatccat acgtatgaag gcggaacaca
    16861 tgaatcagga ttcaagactg ctttaactcg tgtgataaat gattatgccc gaaaacaaaa
    16921 gctgatgaaa gaaaatgacg acaacttgac cggtgaagac gtgcgagaag gattgacagc
    16981 tgttatttct atcaagcatc ctgaccctca attcgaagga caaacaaaaa caaaattggg
    17041 caattccgaa gtacggacag tcactgatcg gttatttagc gaacatttca tgaaattctt
    17101 gctggaaaac ccaagtgtgg gccgtcaaat tgttgaaaaa ggattactag cttccagagc
    17161 tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt tagagattag
    17221 taatctgcca ggaaaattag cggactgctc tagcaacgat cctgaaaaat gcgaattgtt
    17281 catcgtcgaa ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc gagag
//
LOCUS       DACEMO010000039        17063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEMO010000039 DACEMO010000000
VERSION     DACEMO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.040"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..179
                     /locus_tag="IW728_001006"
     CDS             <1..179
                     /locus_tag="IW728_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1024380.1"
                     /translation="SLISRQILYYSFCVPDSFSNYRISREVGYSERSIQRMKSEALIE
                     FAEAYKHGRIIAYK"
     gene            192..578
                     /locus_tag="IW728_001007"
     CDS             192..578
                     /locus_tag="IW728_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024381.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1128..1352
                     /locus_tag="IW728_001008"
     CDS             1128..1352
                     /locus_tag="IW728_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024382.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1370..1798
                     /locus_tag="IW728_001009"
     CDS             1370..1798
                     /locus_tag="IW728_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024383.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1776..3194
                     /gene="terL"
                     /locus_tag="IW728_001010"
     CDS             1776..3194
                     /gene="terL"
                     /locus_tag="IW728_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ1024384.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3206..4741
                     /locus_tag="IW728_001011"
     CDS             3206..4741
                     /locus_tag="IW728_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1024385.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4821..5699
                     /locus_tag="IW728_001012"
     CDS             4821..5699
                     /locus_tag="IW728_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ1024386.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5701..6018
                     /locus_tag="IW728_001013"
     CDS             5701..6018
                     /locus_tag="IW728_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024387.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6067..6768
                     /locus_tag="IW728_001014"
     CDS             6067..6768
                     /locus_tag="IW728_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ1024388.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            6783..7673
                     /locus_tag="IW728_001015"
     CDS             6783..7673
                     /locus_tag="IW728_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ1024389.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7696..7911
                     /locus_tag="IW728_001016"
     CDS             7696..7911
                     /locus_tag="IW728_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024390.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7923..8264
                     /locus_tag="IW728_001017"
     CDS             7923..8264
                     /locus_tag="IW728_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024391.1"
                     /translation="MDESLKTEIIESTKEDFTDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8264..8635
                     /locus_tag="IW728_001018"
     CDS             8264..8635
                     /locus_tag="IW728_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024392.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8628..9023
                     /locus_tag="IW728_001019"
     CDS             8628..9023
                     /locus_tag="IW728_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1024393.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            9025..9402
                     /locus_tag="IW728_001020"
     CDS             9025..9402
                     /locus_tag="IW728_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024394.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9403..10011
                     /locus_tag="IW728_001021"
     CDS             9403..10011
                     /locus_tag="IW728_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1024395.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            10011..10352
                     /locus_tag="IW728_001022"
     CDS             10011..10352
                     /locus_tag="IW728_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024396.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10594..13383
                     /locus_tag="IW728_001023"
     CDS             10594..13383
                     /locus_tag="IW728_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ1024397.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSKAINRVKEIFDSLREINLFEIGKNIIDGLINGVVEKWNALKKTI
                     KGIAGSIKDSIKGALGIHSPSRWMRDMVGKNIVQGIIVGIDKEKSKLDQTMTDLVKTP
                     SVQPVITGSNSQPVVQAKQNTSSNAVNEIHLHLNVYGDLPDSMIKQIAKKMKTELTRQ
                     MKRDADAVGGTLYAT"
     gene            13373..14113
                     /locus_tag="IW728_001024"
     CDS             13373..14113
                     /locus_tag="IW728_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1024398.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKAYYKNVTRKIECYYKAPSIDLVQEWEDRITEWLDMNSYSDFILYYDQQYIYQ
                     AIVTEAPEFKGTRKTGNIVPFEFTVSLRPFKENYSGRFAIQQIKAFELFNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            14110..16821
                     /locus_tag="IW728_001025"
     CDS             14110..16821
                     /locus_tag="IW728_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1024399.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAGVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSMIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEKRAWKIWTMLKARGYSEYAAAG
                     ILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPLVGSPTWNGREYVQRLMNTAGI
                     QEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKSINDAKSAAYAFEMNFERPASA
                     HPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTVTSECGWRTSPINGGQEFHNGI
                     DLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIKHNNGKWTGYAHLSRIDVSVGQ
                     KVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVDFENPRNYIQF"
     gene            16834..>17063
                     /locus_tag="IW728_001026"
     CDS             16834..>17063
                     /locus_tag="IW728_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ1024400.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKI"
BASE COUNT         6079 a         2422 c         3581 g         4981 t
ORIGIN      
        1 ttagtctgat tagtcgtcag atactctact acagcttctg tgtgccagat agcttctcaa
       61 actacaggat tagccgtgaa gtgggttatt cagaaagaag catacaaagg atgaagtcgg
      121 aagctctaat agagtttgca gaagcgtata aacacggaag aataattgct tataaatagg
      181 gggaccatat aatgtggaaa gattatgtat cgttgaaaga gttgaaaaaa gatcttgttt
      241 tcaaaagaat tgtagaatgg tcagagagtg aattaatttt agaagatgga accaaaatgg
      301 aagttgtatg tagcgaatca gattgctgcg catgggccga aggtgaattc aaaaatgtaa
      361 agcttgatgc agtgattaca gatataaaga tttttgataa aggtaaccgt ctctataatg
      421 gtgacggaca tacctcttat gctgaagtcg ttgtttatca taatcgtaat gagatcgcta
      481 aagcagaatg cacagcgaat gatgggaatg gcggctacta ttatagtgtt tgtgctttaa
      541 aggtcaaaga caaactttgt atagtgactg acgcataaaa aatggcggtt ttttggcgga
      601 atgatggcgg tttttagcca tttatcagtg atattatggt agtgtcgaaa gattagtgat
      661 aggtctgaga caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc
      721 ttcgatagac agcaacggaa atattaagaa taaggatgtg aattttaact ccttctaaat
      781 tgttcttatt atctatcatc cgttgctgtc tattgttaca tacagatcgc ttaggcggtc
      841 tttttatttt ggccgaaaac ctacattttc gatggccgat tattggagga ggaatagcat
      901 gttcaaacta tctgaaatca ttaagaagtc cgacgttgag aaattagaga tgttaaaaaa
      961 gaagttgaag aaatagcagt ctcttcgtga ggctgtttta ttttgctcac aaaaatagac
     1021 cactatcggg taatagtggt caggaattaa atgaaaaaga tgttagggtt gttagctaag
     1081 tataacatca taacgcttac aaaacaatgc aagaaggata aattattatg agaacttact
     1141 ggtatgtgtc attaaacaat aaatatccga agccaatgaa aggacagcat aggcgtgtag
     1201 tcatgtctgt tcaaatgaag gcgaagtatt cgattgtaga aatgatcaga gaggcaacgc
     1261 cagtagagat tgattattgc aagctagtct attgtgggtg cggccgttgg aaagaggatc
     1321 atgtacaaaa aaatatcagc aagtacatat agagaggcgg tgattaagga tggaaaaaag
     1381 cgatacgaaa gcgaacagat acaaacctac tgctgcagag aaaaaactct tggaagtatt
     1441 gataaatcca gataacttag gcaaaccggt gcaagagttg tgttcactgg cgaaaatagg
     1501 taggaccaaa tattacgacg ccatgagcaa agatggcttt gttgatctag ttaacgaaac
     1561 aacaatggat ctgatcaaag gaaaagcttc tgatgtcctt aatgccactt acaagtatgc
     1621 attgactgaa aaaggtcatc aggataggaa aatgattctg actattgctg gaatctatgc
     1681 agacaaacaa gaactcaaac attctggcgg cgtcgatatc cgaaaacagt atgagaaaat
     1741 gtccgatgaa gaattagaag agttggtgaa acggtatgaa aaaatcaacg actcttaatc
     1801 gtgttgagta tgccgttgtt caaagagagc tagccattcg aaaagctagg aaaagttttt
     1861 ggctattctg ccagcttctt tatccagagt tttacaaaga tgatcggttg tatttgaaag
     1921 atttatgtca tacgctacaa gatttttacg aagataaaat cgacaaaaaa attttggtta
     1981 ttaacatgcc gcctcgacat gggaagactt ttacagcgcg gctattcgtt ttatggatgt
     2041 ttggacaaaa tccaaagacg aaaattatta ccggatctta taaccaaatc ctgtcatctt
     2101 tatttgcgca acaaacacgt gatggaatca tggttgaaaa cgaaaatgtt aaggctgaat
     2161 actttaaaga tatttttcca gaggtgcaaa tcaaatatgg agatgctgca aagggtttct
     2221 ggtctttaga tggttcagaa gaaaagaatt atttagcgac ttctcctggt ggaacatcca
     2281 ctggtattgg cgcgaattat gtaattgttg atgacatcat taaaaccgca gaagaagcct
     2341 ataacgagcg tgttttagat tctcactggg aatggtataa caacacgttg gcccaacgaa
     2401 tggaacggcc acgtaaacag atattgatta tgacacgttg gtcaagtaat gatttagccg
     2461 gaaaaatgtt gacgagacga aaaaataatg ttcatcagat ctgttataaa gcagtacaag
     2521 aagacggctc gatgttgtgc aacgagatta tgacccacga tgagtactta gatgtggtac
     2581 aagaaatgaa tgtagatatt gcagaagcca actatcagca agaaccaatt gaccaaaaag
     2641 gcaggctata tcagaagttt cttacttatg atacgttgcc tgataacatt atcaaaatat
     2701 ggaactacac cgacaccgcc gacaagggtg ccgattactt tgcgtctcct gtttttgctg
     2761 agacgtctga tcatgatgct tatattatcg atgtcatgta tacaaaagag ccgatggaaa
     2821 caactgaaat agcccacgct aatatgatta ttcgcaatcg agttaataag gtacggattg
     2881 aaggaaacaa tggcgggcgt ggattcagaa gaaattcaga aagaattaca aaagagcgag
     2941 gctatcgagg tgcagtattt gaagactttc atcaatcgga taataaactg tctagaatac
     3001 tggcgaatag tgcttgggtg gaaaatcatg tttactatcc agatgattgg gcgacacgtt
     3061 ggccggactt ttatgaggct atggttagtt atcaacgtga aggtaagaac gctcatgatg
     3121 atgctccgga tgcagttaca ggaattgcag aaacgattat agaagcagat gacttcgagt
     3181 ttattgctta ttaggaggtg ggtctttgtt ttattctgga atcacattga aaaaatacaa
     3241 acgtttaaga acgaaatatt caacgcaaat cgaagaagga ttattcgatc ctaacgcttt
     3301 tatggaagat atgaaaccct ttttcgatga tcgagaaaga aagtatcttg cttatactag
     3361 cgagaaaaac gagatcgaca gtcgtgagaa accaaaaaca aaaatcatta aggtcaataa
     3421 caagattcat gcgggaatgt attcagtggt cgttgatcaa gcagttaacc atttcacagg
     3481 aataccaatt aaatgggatt atgatgtttc ggaacagaaa aggacattca ttcaacgaat
     3541 gagagacaaa ttactgggta atgatatcag cgccccttct accccagaag aatttgaaac
     3601 actcacgaga caattaagta ctatgcggtt tggaatgtta gaccccgata ctgcaacatt
     3661 tcaaggagcg acagggcttg ctttcagatt actggagcct gttgagacaa atgatggttg
     3721 ggaactaaga gcaagcaaca ttgagccgtg gaaagctgaa aagtacgaaa acggcgcagt
     3781 gtacattaaa gagaaatacg atccatatca aaagaagttt tatgaagaga tgaaggttgt
     3841 cacgccgaaa tcaatattta cttatgctcg atatactgat taccctggca cggtacaagg
     3901 agcatttaaa acgctgggta atgttcctaa tcctttaggc gttatcaatc ttacggagtt
     3961 taaaaacaat acaaaccgtt actgtgactt tgaagttgca gaggagatta gtgatgctat
     4021 tgatcgtgtt ttatctgatc agcagaacga gattgaacaa ttcaagttag cttatatgct
     4081 gatcactggg gcagatttgt ccgaggataa agccaagaag atgatggagc aactagggat
     4141 catcaatctg aaagacccta atgcgaaagc cggctacgtg acaaaggatc tacaaaagga
     4201 tttcaacgag tatcattttg atcttcttaa aaagcaattc tacacggtgt gtaaagcaat
     4261 tgatttcaat gacgaagtct ttaagtcgaa tagttctggg gaagcccgaa agtggcaaat
     4321 cattagttta gaagctaaga caaataccaa agaacagtac tttcgagaag gattgaaaga
     4381 gtgtgccgag gttatggctg cttatttgga ttttgccgag aaagttacaa ttgatccgga
     4441 aaagatcatt ttcaccttct ctcgttcgct tccaacagat ttaagctacc tagctgaggc
     4501 attgcctaag ttggcaccat acgtttctaa gaggacgatt caaagtcaga tcccgttcgt
     4561 cacagatgtt gattatgaaa acgaaatgat tgacgtcgaa agcagacaat cttacccaga
     4621 cggcgagtat ggaggtggca tcgttgagtc aaatagaaac gaagtactgg accaaacgtc
     4681 gagaactgga agatttagca agactcaaaa tggaaaatca aacgctaaag aaactaagta
     4741 gtgtctttcc tgatgcgttg aaagaaattc aagcggagtt attatcccaa gctgatttac
     4801 acgatattac ctatgctgag atgatggaat tttatagtac aagtgatcag aaaaaatatc
     4861 gtgaatacgt tgaaaagaac tacaaatcac tgaagatgta tgatgcaaaa tacaaagagt
     4921 tcatcgaaga atactttcct tctttcgact acgccaaagt gaatcggctg cttcaaatga
     4981 ggtcagatat ttttcgaatt cttgcagaga gtgcaataga tgcagatgtg aataaatatt
     5041 tttcggatcg gttagaagat atcttgcaac gttcgtatgt atcaaacgca aacgtatttg
     5101 cccagatttt gaacgtagat ttgccgaatt acttatcaaa ggaagaactt gaacgttacc
     5161 tgaactatcc ttggtcaggt aagacattct cgcgtcgttt atggggcaat atttcatctc
     5221 tagaacaaaa gctatctaac gccattacaa ctagtgtagc aagtggcgaa ggagttctcg
     5281 tggcattacg aacaatgcga gaagacccag aaatatgcga tatgttcaaa agggaggaat
     5341 cgaagtacaa caaggcgatc gaaaatcttg tgagaacaga atacgctaaa ttcgctcaag
     5401 atggtattga gaaatcttat attgaaactg gcgtagacga atacaatgtc ttaactgcaa
     5461 aagatgaaaa ggtatgttca atatgcggtg gcaaagcaaa gaacaaccca tacaagttga
     5521 gtgaagcgat cattggtgag aaccgtgcgc cttttcatgg ccgatgcaga tgtacagatg
     5581 ttcctaatat gccgaaatta ggaaaagata ttgacgaaga gtatgagcgt ttatttggcg
     5641 atttattaga tgagtttgca catgattctt ttggaattaa tttgaaacgg aggaagtaga
     5701 atgaaagatt tttttgaagc agtactaaca attaatgtaa atgctgatat tgcagaagcc
     5761 tacaaaacag ctattgagtc tgagaaccat cctaatggct tgagagacca ttggaatggc
     5821 aattatgcct acgtggttat tggcgatcaa actgttaatt atcaagataa tactccagtt
     5881 gataagaata ccgttaattt aacgattcaa ttattatctc attcattacc aaatttaaaa
     5941 gaaacagttg attggtatga aaatatggga tgtattgttg ttagaactga ctacaaagaa
     6001 ggaaagtcta gtaattaggc ttttttattt tgtccgaaat gacgttaaac tagcgcaata
     6061 ctgggcttgg ttgaatggtg gggcgcaact attaaaactc aaagcaatgc ggggcgtgaa
     6121 aacgaatcgt ggggcgaaag gagaatgatt atgaaacaca aatcattaat gccaatgaat
     6181 ttgcaatact ttgccgaagg tgacgatcaa aagttttctt ttgatgactt caaatctttt
     6241 gtggaatcca atgaagaagc tcaaaaattt attcagtcac aatctcaaag tgttgccgac
     6301 aaacaattgg aagcttggaa acaaaataac ttagataaaa ttaaacagga taccatcaag
     6361 gaatatgagg aatctaagaa aaacaagtca cctgagcaaa ttcaactgga aaaactacaa
     6421 gctgaatttg aagcagaaaa agcgttgcgt gtgacaagtg ataataaagc atttgttgca
     6481 gaacaaattg ctggattaga actagatgga gagttaaaag agtctatttc tcaatttatg
     6541 ctaaataatc ttgttagttc ggatacagat ttcactaaga atgctgttga aggttttaca
     6601 agtgttttga atgcaattaa tgagaaacat gcagatgcat taaaagaact acaaatgaag
     6661 tctgcatttg gtggaactca acaatcgaat aaccaggttc agcagaacaa tgaaacatta
     6721 acaaatccag aagaacaatt aggacaaatt cttcaacaat ttaactagga gagtgaaaaa
     6781 ttatgaaaaa aacatcttta aataatttag agtatttgga tatttcacca gcgattaatg
     6841 ctatgcaagt accaaataca ccttttttaa gctatttact tggtgctgga aaaacagagc
     6901 cagcaaactc gacagaaatt aaatggcgtg aatatgatat caacaacgat gattcttctg
     6961 aaaaacttga gggcggagaa tatccagatg ctgaatcagg tcgaacttgg tttaacaact
     7021 atactgaaat ttttagaaaa tcaacctctg tatctggtac attagatgct attaatgtga
     7081 atggagtcgg aaatgaatta actaatcaag tagcactacg tggtatggaa atgaaaattg
     7141 atttgaaccg aaaattgatt actggtgtaa aagctgatga aaatggttct aaaggtcgtc
     7201 gaatgaatgg gattttgaac ttgatcaatt cagcaaataa ggcagaaaca gccactgcgg
     7261 gtgcagtaac aagaaaagat atcgatactt tatttaaatt gatgtatgaa aaaggttata
     7321 tgggagaaaa actatgcttg atttctccag atatgcagga gttaatgact gatgagttag
     7381 atggaaaatc aacaaaaatt gttcagttcg gggaaagagt aacttttgga ttgcaaattg
     7441 gaaatatcgt gtctaattac ggtacaggta ttgctctact agaaccatca ttgccaaaag
     7501 gaacaattgc cgcaatcgat actaattatg tgaaactacg tccattacgt gaatggagag
     7561 cagaagaact tgcaaaaaca acggattcta gacgtatcgg tcttgtaggt gaatactctc
     7621 ttgaatacaa tgcttcaaac tctggggcaa ttttaaattt aaagtctgaa taaaagggag
     7681 ttagtactcc cttttttgat aggaggaatt acagtggtaa aaaaagatga aactaaaaaa
     7741 gatgaagtcg tgaaatatag agtaggtaaa actaaaaatt ttgttgggtt tgttcatcct
     7801 aaaactcgta gatttatcac agcagattca aataacgaat ttatcatttc tatagatgat
     7861 aaagaagcaa ttgcaatttt ggaagatgca attgatgtta atgaaattta ggaagtgatc
     7921 tgatggatga atcgctaaaa acggaaatca ttgagtctac aaaagaagat tttacagatt
     7981 tgagtgaaga acgcataact aatttattag aaataatttt gctagaaatt gaatcataca
     8041 acacttgtaa aaatgatatt tcatgggaaa agttaaaaag cgtgattagt gaagtgttgt
     8101 ataaaataat aaaaaatgaa tcagaaaaaa cagtatcttc agttagacgt ggcgatacaa
     8161 cgattagtta tgcttcaaca acaaatgatg ttagtgagtt acttctaggc tatggtgatt
     8221 tgatacgaag agttattggt tgtggaggat tggagttttt ttaatgaatg aagcagatat
     8281 tttagaaact acttatgaag atagttgtat tattgaaaga ctgacggaca ttgaagattc
     8341 taatacaaat attactattc aagattacaa aaaagtatat gataatccta tttcttgcgc
     8401 tctttcacaa ggtcaaatcg atggactagc agtcatagaa gatggagaga tggtaaatgt
     8461 ttcaactgac acatataaat tatttgttca tcctaagatt aaactaaaaa aaggagatcg
     8521 aataacaata actcaaaagg ccagtggctt aattttttct ctatttgcta ctaagccttt
     8581 ttactatcct agtcattgtg aagtgaattt gataggaagt gagaaaaatg ggtaatctca
     8641 aatatgaatc aaatgcagaa aagatcattg agaattttaa aaatatgact gtaattgctc
     8701 aaaaagaagg aatatccttc gttaacgatt caatgaataa agtcgttagt ttagctaaac
     8761 cattaactcc tgtaaaatca ggtaatttaa ggcgaggata tcgtgtagta aaagctagaa
     8821 aactatcaag cggtcgaatt gttggagcag ttataaacaa tgaacattat tttaaatatg
     8881 tagaagaagg tcataggact aaaaatggtg gctttgtcaa agggaaattc atgttaactc
     8941 gtgcaacgaa tcttgcaaat atgtcttata ttcctcgaag atttaaacaa atggcaataa
     9001 aaatcgttaa gaaaggaaag taacatgaaa gatgaaatca ttgctgcaat cagtagcaaa
     9061 ttaaaagaaa tctatcctga tgggacaata tatcttgatt cagttatgca gtcaactaaa
     9121 gatttttatt tcgttttatc agtaatggaa tctggaactg aaaatgtagg aattgatatt
     9181 caaaatgttt ctttcttaat tgatattgca ttgattgata ataaacctaa tagaaattta
     9241 attaatgaat tagtctcacg ttgtgggact ttttttaata cgattacaat cgatgagcaa
     9301 atattatttc caaaagccta tttacctgat gaagcagatg gtgttcaaca tattcgtttt
     9361 acattagaat ttccacaata tattgaatgg agtgaaagat aaatgggaga aaaaagaagt
     9421 aaaaccggaa ttatttctgt cgaaaaacct acttggtttc ctttggaaga tgagacaggg
     9481 aattttccgg tatatggtac tgcgttgcca atgggaacag cagtaagtat caaaccaaca
     9541 gctaattatg aaacaacaca agattatggg gattcagtgg ttcaagatca atttacagct
     9601 tttggtggtg cagaagttga actggaagca aatggatata ctcataaagt tttatctaca
     9661 attactggag ggaaaattgt taaaggtggt gccttgcgtt ctggagaaga tattgcgcaa
     9721 gatggtgcgt ttgcatatag acgtaaaaaa tcgaatggga aataccgtta tacggtcttt
     9781 tataaaggac agtttgctct agattcagat gaaacatcaa cgattgaagg aagtaaagta
     9841 agttttactc atccaacttg gaagggttca tttgtagatg taccaggact tggatatatg
     9901 tattcagttg atgaagatga tgaaggcgtg gataaagcaa tgatcggaaa ttggtttaca
     9961 aaagtagcaa ttccaattga agaagcagaa ctttcaggag gtacagaata atgtcaaaat
    10021 atcaaacaac gattaaatta acgaaaaaag atgaagaagg taaatatgag caagtacaat
    10081 ttaaatctgc tgaattttta ccaggaacag ttgtagaaga tgctgcagga gttatggaag
    10141 aaatgcaaac agcgactgat aaacaatcag ttaaaaaagc tttaagtcgt gcttattcat
    10201 ttattgcaga tactttattt gagggacaat ttactggtga agattattgc aaaggaattg
    10261 atgctcgtga gattgcctca ttgacaggaa aattattgaa gtctgttact gcaggttttg
    10321 atgaaactta tacagagacg aaaaaaaagt aagtgaggcg ctcaagtcac cttcatttaa
    10381 gtattcgatt acttaccgag aattagatat aaagacgcaa ttactcgaag caggttggac
    10441 gttaccagaa attgaacgta ctgacttgga tgagttaatg cgtctttttg cttttagaga
    10501 tgcggtaaaa gagcatgaag atgttgagta ctatgataat ttcactcaat tttaggaggt
    10561 gatactttga ataacgatga cctaattctg aagatgatac tagatgaatc tggttttact
    10621 gccggtatga ataatgctgt caaaaagttg aactcttttg atgaaacaat cgaaagaaaa
    10681 agtagaaata gtggcagttc attaggtagt atttggaaaa tatttgctgg aagttttcta
    10741 gctagcggag taactagaat cgtaggagct ggctttgatc taatcaaggg ttccataagt
    10801 ggagcggttg atcgagtaga tacgatgaat aacgctctac gaaatttcca aaatatggga
    10861 tttagtaatt cagaaattat gaagaatatc ggaaagaatg ggcttttatc tcaaggtatt
    10921 caaggacttc ctactgcttt gaatgatgcg ataagtcatg ttcaacttct tgcttcttct
    10981 acaggtgatt taactcgttc aactcaaata tttaaagctt taaatgatgg aattcttggt
    11041 ttcggtggtt caactgatca agttaatgaa gctgttattc aattatctca aagtttctca
    11101 aacggaaaag tagacgcaca aacttggaac tcaatgatca acgctcaact tggacctact
    11161 ctttctgcta ttgctaaaaa gatgggaatt acaatgggag atctgaaaga aggtttgtct
    11221 caaggtaaga tttctgttga agaattccaa aatcaattaa tagaaatgga taccaaaggt
    11281 ggcggaggac ttaaatcatt aagtcaaatc gctaaagatt ctactaaggg aattaaaacc
    11341 tctatacaaa atgcaaaaac agctgttaca cgtggtgttg gtgaggtaat agaaggatta
    11401 aataaagcat tagtagattc agatttaggt ggatttaagg gaattattga taaagtgggc
    11461 agttcaatgg aatcgttctt aaaagtaatt gctgcaaata ttcctacagc agtatcattt
    11521 ttaagtaatt tatttaacga aattcaaaaa tttggttctg ctttgaaatt catgatgccc
    11581 tttcttgttc ctgcagctac cgcttttgga gcgttcatgt ttcaacttaa aggtatacca
    11641 gcaataataa aaagcttcaa taattttaag aatgccataa ttggcgttgg aaattcatta
    11701 aagattatgg gggcaatagc tgctgctaat tcatttgttt taattattgg tgcagtcgtt
    11761 ggtgcaatag ctgttttcgg ttactttatg gcaaccaatg aagagtttag aaacaaggtt
    11821 atatctattt ggaatgatgt gaaagattcc gtaattggtg tattaaaaaa tataaaggac
    11881 tggggaattg atacttggaa ttctgctaaa gaaatggcat caaatgcagt tgagggtgtc
    11941 aaagatgctt ggtcaggaac aaaggaatgg ttttctaaca cctggaaagg tattaaggat
    12001 ggggccacag gtttatttga taaaacagta gagacttcaa gaaatgcagt cgatagtgta
    12061 aaaaatgcat ggtctaatat gaagaaatgg ttttctgata cttggcaaag tataaaagat
    12121 tcagccagag aaaaatggaa tgaaattaaa ggttctatta tgtcggttgc tggtccattg
    12181 attacaggta taaagaatgc atttttacat gttacttttt atctagagac cttatggaat
    12241 aatttagttg agattggtaa aaacgtcttt gagattttaa aaaatgttat acttgcgccg
    12301 gttttgttta ttacctccat gatttctggt ggatgggaag aaacaaaaaa taatatgatt
    12361 gctgtttgga ataatattaa agaaagtgcc ataaatattt gggaatctat aaagaatata
    12421 tttgtaagtt attttacaaa tatttatttt gctgcactca atatttggac agggttcaag
    12481 cttacattga taaatatttg gaatgaagtg gtaaatcaag ctaaatcgat ttggttaaac
    12541 ataaagtatt tttttattaa tctttggatt gatataaaat attttgcaat tcagaaatgg
    12601 attgaattga agttttctat tattcaaact tggatcgatt taaaatataa tgccattact
    12661 acttggaata atattaaaca gtttttcaaa gatacttgga aaaatattaa agatacggca
    12721 tacaatacgt ggatttctat aaaaaatacc atgattaata catggaataa cattaaggac
    12781 tctttctgga atattgttac tggaattgtt aactccgctg aaaatgcatg gactaatcta
    12841 aaaaatggtg tttcaaaagc aattaatcgg gtgaaagaaa tctttgattc attaagggaa
    12901 atcaatttat tcgaaatcgg taagaatatc attgatggac ttatcaatgg tgtagtagaa
    12961 aaatggaatg cattgaaaaa gactattaaa ggaatagctg gaagtatcaa agattctatt
    13021 aagggtgcat taggtattca ttctccatct agatggatga gggatatggt aggtaaaaat
    13081 attgttcaag gtattatcgt tggtattgat aaagaaaaaa gtaaattgga tcagacaatg
    13141 actgatttag tgaaaactcc gtcggttcaa ccagttatta cagggtctaa tagtcaacca
    13201 gtggtacaag ctaaacaaaa tacatcttca aatgcagtga atgaaattca cttgcattta
    13261 aatgtttatg gagatctacc tgattcgatg attaaacaaa tcgccaagaa gatgaaaaca
    13321 gaattgacga gacaaatgaa acgagatgct gatgcagtag gagggacatt atatgcaact
    13381 taaaagagga caatttttta tcaatcaaca ttattcttct gagtttaacg tatatattca
    13441 aaataggcct gcctctgttt cagctagtcg tgtaattgaa ttgagagaac gtgaaggcaa
    13501 tgactcaatt attatcgata aagcctatta caaaaacgtt actagaaaaa ttgaatgtta
    13561 ttacaaagca ccatcgattg atttagtgca ggaatgggaa gatcgaatta ctgaatggtt
    13621 agatatgaac tcttacagtg attttattct ttattacgat caacaatata tctatcaagc
    13681 tatagtaacc gaagcgccag aatttaaagg aacaagaaaa acagggaaca tagttccatt
    13741 tgaatttaca gttagtcttc gaccattcaa agaaaattat agtggtcgtt ttgctattca
    13801 acagataaaa gctttcgagt tatttaatcc agagaagtat gcttcaaaac cgcttattaa
    13861 attgagtggt tctggagatg cttcttttta tattaataat gataaatatg atttgaaact
    13921 attagataga gaattatata tagattctaa actagaagag tcttatcgaa agctagatga
    13981 caatttagaa catcaagatc atgtcacttt atttttagat tttccatttc tataccctgg
    14041 aaaaaatgaa atcaaatgga cgaataacat acattcgttt gagataatac caaggtggtg
    14101 gagaaaagta tgataccaag aatatatagt cctaccgaga cagatttttc tacgaatggt
    14161 ttagggattt tgaaggacac cacaaaatgt gagatatatg aagtagcaaa tggaaaatat
    14221 gaattagagt tggagtatcc attaggtact cgatttgatg aatattttga aaatgactat
    14281 caaataaaag caaagtcaaa tgatcaagaa gagtatcata tcttttttat tgatgataaa
    14341 gatattgata cctttttaga taccgtaact atttatgccc agagtcgtac aaatcggctt
    14401 ggaagacgag cggtcacttt tgctggagtc gactctaaaa ctggtcgaga agcaatggca
    14461 attattgaaa acaatatgga taaaaaatca gatatacgct tatattctga tattacaact
    14521 gtatcaagta caacttttga agcaagaaat gttttaaatt gtattgctgg tgaacaagga
    14581 tctttacttc agtattgggg cggagaaatt aaacgtgagc cttttaagct atctttatta
    14641 aagcgaagag gtcgtgataa tattggcaca atccgatatg gaaaagattt atctgggtta
    14701 aaagttaagt tagattggac aggcgtgaaa acaagaatta ttccgtatgc tgatcctcaa
    14761 agtgatgcag gtacaactag ccgaatttat ggctcgccag tagatagcga atatattaat
    14821 aattatcctg atgtatatac agaacacgtt caattcacag aagaacaggg agttaaagac
    14881 gttaatagct taaataaaat agctaagaat tatttcaaaa caattaatcc aggctgtgat
    14941 aaaccgaaga tttctattac cgttgagttc gataaattaa cggacaccga agaaggaaaa
    15001 gaattcgcta agataagaaa ttatgggtta tttgatacgt ttaaaatata tcatcgaaaa
    15061 tataaactat attttgaatc gaaagttagc ggtgttcaat atgattcatt atctgaaaag
    15121 gttttgaaat tggaagctgg ggatgctcaa gttgcttttt atcaacaaca agctgttact
    15181 attcaagata aattaaaaga ttatgctacg aataattata tgagtagttt taatgattat
    15241 gtgtcctcta tgattgcagg tcaaggaaat gcaggcggtt atgtagtttt atggccaaaa
    15301 gaaaaacctt caaatatttt tataatggat agccctgatt taaacaaggc aaaagaagta
    15361 ttaagaatga acaaaaatgg aattgccttt tctaaaaatg gttggaatgg tccttttaat
    15421 tccgcttgga cattggatag tatattcaat gctaatttta ttcaaacagg attaataaag
    15481 gcagatatct ttcaaaactc ttttaataaa actggagatg tattgaaatt agttaatgga
    15541 ctacttcaaa tttggaataa caaaaagaaa ataatggagt taaccaaaaa aggaatggag
    15601 ttttggaatt ctaatagttc aattggaacg attggaacaa ctgattctgc tggtaatcct
    15661 tttcctggag cttctactcc cacacctatt gaagataatt ctttagttat tcgtacaaat
    15721 ggagacggca aatatatttt gatttctcct aaagttggta aaggattagt tttattagga
    15781 aatggtaaag caatttattt tggagactta gatgtacaag gcaaactcac agttaacgga
    15841 aaagaaatca cagggaacaa cagtggagga agtgatcctg gaactatccc tcctcaattg
    15901 acgacggaag ctgaaaaaag agcatggaaa atttggacaa tgctgaaagc tcgtggctat
    15961 tctgaatatg cagctgcagg tattctagga aatattcagg gggaggttgg agcaagtatg
    16021 aaccctgata cagaacaact tggtggtcca gcttatggga ttgttcaatg ggatggttct
    16081 gcttatccgt tagtcggatc accaacttgg aacggacgag agtatgttca gcgtttgatg
    16141 aacaccgcag ggattcaaga agattatcga agtattgaag cccaagtaaa attattagat
    16201 tggtgtatgt tcaacggtca atggctcgga aaagtaaatc caaccacagt atcaggattt
    16261 aaatcgatca atgatgccaa aagtgcagcg tatgcttttg aaatgaactt cgaacgccca
    16321 gcttctgcac atccagaacg ccaaaattat gcccaatctt ggtataacaa attacatgga
    16381 ttaactagtc cagaacctgg agggaatttc atttgtccaa ttcaaaaacc agtgacagtt
    16441 acttcagaat gtggatggag aactagtcca ataaatggcg gccaagaatt tcataatgga
    16501 attgatcttg taaatggaaa tcctaataca cctgtttttg cagcattaga tggggaagtt
    16561 gttcaggctg gtgctaatta ttatgactgg tatggtaatt acgtggttat taaacataat
    16621 aatgggaagt ggacaggtta tgctcatttg tctcgtattg atgtttctgt tggacaaaaa
    16681 gtccagaaag gtgctcaaat aggcttgatg ggaacaactg gtccatctac aggagaacat
    16741 ctacattttc aaattatgaa aaattattgg ccacagccag ttgttgattt tgagaatcca
    16801 agaaattata tccaatttta aggtggtgat tctatgagta aatggaatgt cgttttaagt
    16861 acaacagaac catataatta tgtggggatg attcaagttc gacaaggcaa taagaataca
    16921 gaggttatgg aagcgactat agttgaaaat ggtcttccct acgatttatc agaatgtaag
    16981 gtatattttg aatcagttgt aggtgggaaa tatccagtcc aattagaaac aaaaattgtg
    17041 gatgctaaaa aagggaaaat taa
//
LOCUS       DACEMO010000040        17040 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEMO010000040 DACEMO010000000
VERSION     DACEMO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.041"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1230)
                     /locus_tag="IW728_001027"
     CDS             complement(<1..1230)
                     /locus_tag="IW728_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1024401.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAA"
     gene            complement(1223..2491)
                     /locus_tag="IW728_001028"
     CDS             complement(1223..2491)
                     /locus_tag="IW728_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1024402.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2743..3204
                     /gene="mscL"
                     /locus_tag="IW728_001029"
     CDS             2743..3204
                     /gene="mscL"
                     /locus_tag="IW728_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1024403.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3263..3709)
                     /locus_tag="IW728_001030"
     CDS             complement(3263..3709)
                     /locus_tag="IW728_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1024404.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(3802..4047)
                     /locus_tag="IW728_001031"
     CDS             complement(3802..4047)
                     /locus_tag="IW728_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024405.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4162..4740)
                     /locus_tag="IW728_001032"
     CDS             complement(4162..4740)
                     /locus_tag="IW728_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1024406.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(4817..5347)
                     /locus_tag="IW728_001033"
     CDS             complement(4817..5347)
                     /locus_tag="IW728_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1024407.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5351..6211)
                     /gene="rlmB"
                     /locus_tag="IW728_001034"
     CDS             complement(5351..6211)
                     /gene="rlmB"
                     /locus_tag="IW728_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1024408.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6204..6599)
                     /locus_tag="IW728_001035"
     CDS             complement(6204..6599)
                     /locus_tag="IW728_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1024409.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6596..8008)
                     /locus_tag="IW728_001036"
     CDS             complement(6596..8008)
                     /locus_tag="IW728_001036"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1024410.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8005..8541)
                     /gene="cysE"
                     /locus_tag="IW728_001037"
     CDS             complement(8005..8541)
                     /gene="cysE"
                     /locus_tag="IW728_001037"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1024411.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(8863..10323)
                     /locus_tag="IW728_001038"
     CDS             complement(8863..10323)
                     /locus_tag="IW728_001038"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1024412.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10493..11650)
                     /locus_tag="IW728_001039"
     CDS             complement(10493..11650)
                     /locus_tag="IW728_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1024413.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11684..13054)
                     /gene="radA"
                     /locus_tag="IW728_001040"
     CDS             complement(11684..13054)
                     /gene="radA"
                     /locus_tag="IW728_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1024414.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13117..13644)
                     /locus_tag="IW728_001041"
     CDS             complement(13117..13644)
                     /locus_tag="IW728_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1024415.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            13874..14680
                     /gene="proB"
                     /locus_tag="IW728_001042"
     CDS             13874..14680
                     /gene="proB"
                     /locus_tag="IW728_001042"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1024416.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14677..15924
                     /locus_tag="IW728_001043"
     CDS             14677..15924
                     /locus_tag="IW728_001043"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1024417.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16000..16344
                     /locus_tag="IW728_001044"
     CDS             16000..16344
                     /locus_tag="IW728_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024418.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16461..16787)
                     /locus_tag="IW728_001045"
     CDS             complement(16461..16787)
                     /locus_tag="IW728_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024419.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
BASE COUNT         4628 a         3663 c         3125 g         5624 t
ORIGIN      
        1 agctgccagt acgtctttca tttggatcga atcgatgatt tcaggaagat caaataaagt
       61 tcgatcgcca aataaacttt gcgtaaattg attggcaata tattcgatcg aatttaatga
      121 ttggaaatat tgtccaagca ttttcttctt cagcaaatcg agatttgttt ctgacacttc
      181 cggatcatca gcgaatccaa gaatgatttc tttgactttt tctgctgctt tctctggttc
      241 gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga tccaagctga attcaaaacc
      301 aaagctgtca tcgataattc cttgattgta catcgctaga tagtttcgag aagtattgcc
      361 taaaatcatt tggaacaaca gattgattgc tgttttatag cgtaacagtt ctgttccttc
      421 ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat ttatctcgtg taatggctgc
      481 ttccaatgca ctttgtttga tgatttcttt tgtgttttca gggaaataac gtacaatttc
      541 ttgctttgga gggaaatttt ttgcttcttg gttttcccga ataagtttca tcaatttctc
      601 aggctccatc ttaccaacta caaataaaac catattgctt ggctgataaa acgtccgata
      661 gcacgtatac aagtcttgag ctgtgatttt gtcgatactt tcaaccgtgc ccgcaatatc
      721 gatatgcaat gggtgtgtag gatacaagtt atttagtatg ccaaaaaaca tccgccagtt
      781 agggtcgtct tcatacattt ggatttcctg accgatgatt cctttttcct tgtttacagt
      841 ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga gtcgtcagat tcttttctac
      901 ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa cttgtgaaag cattggcgga
      961 agctccttgt ttcccaaata attgaaagac atcaccatct tctttttcaa acaacttatg
     1021 ttctaaaaaa tgggcaattc catctggaac tttcaccttt tctttttcac catagggaat
     1081 aaattcgtta tcgatcgaac cataattcgt actgaacaaa ccatatgtct tgtgatagtc
     1141 atttttaggc aacaaataca cggtcaaacc gtttggcagt acttcatgat aaagtgtttc
     1201 attgatttgc tcatattctg ttttattcat ttgcatttcc tcctgctaaa aagaagattg
     1261 cctgcaattc gatttgctca gctacttgtt gtatctcttt gatcgttacg ctctccatac
     1321 gtttcaacca ttcttcatca gataattttg tttccggcag ccagctgtcc aaaaaagaag
     1381 cttcgattgc cgcttgcgga gagtctagag aaagcagata ttggtttctc agcattgctt
     1441 ttgtctgaga cagttccaac tctgttattt caccatttcg caaactttct aattgctcgt
     1501 gaatcagatg aagtacttga tttcggttct tttcatcgat accggtttgg acactcatga
     1561 atcctcggaa cgtatccacg ctactagagg cataataagc tagactttct ttctctctga
     1621 cattcatgaa aagtttagag tgaggaaaac cgccaaataa accgttaaag accattagcg
     1681 caaaacgttc tggttcatcg taatacacat ttgtctgata agctaggttc agctttgctt
     1741 gaacgattgg ctcacgtact tgtcgttcct tcacgatatt cacttgaggt tgggtataaa
     1801 acagatctgg ataaactgtt tcccgcgact caaatggtaa ttgcccaact gcttcagctg
     1861 ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat ttgatcattt gccatcattt
     1921 cttgatagta ggacgctagc gaactagcag tgatcttgtc caaatcttcg actacgccca
     1981 accctgggat cttttgttcc gggctgtttt caaaataaag ttcttgcaag gcataggaag
     2041 caaaagtttg tttatcttcc ttcaaacttt ccaaatatag acgcagattg ttcttttcca
     2101 agtcaaacgt caactgatca aactgatgat ttttaatatt tggataaaat aaaacctctt
     2161 ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt gacatattca ccattcacaa
     2221 aactgatccc tgcgtttagc caatgtaaat ttcctttttt tctaactgat aatccaaagc
     2281 ttgcgccata aagctctgca agttgcgcac ttaactttgt ctgatccgga taattcaaac
     2341 tatttgtttc caacatgctg ctgagaagtg aacgtttcgt gatgacttct tgttgcagac
     2401 gcgcagtaaa acggataaaa atccggatcg ttttgtattg ttttgttggt aagacatgaa
     2461 gattcacgcc ttctgccaat tgatacacca tatttatatg ctccttttca cagttgattg
     2521 cccgcttact cttttaatgg gtcaacctgc cttttttact tattcctctg cttgaaattt
     2581 ataagttcag gcaattcgcc gttttttcta aatgatgtta cactttatag tatagcatag
     2641 atgtttttta tcaaaagaaa cagacggcta aaagcgtata ttgttttttt attttttatt
     2701 tttcgatata ctaaagacaa ataaaagaag ggagggtcat tcatgatcaa agaattcaaa
     2761 gagttcatca tgcgtggaga tgtcttggat cttgctgtcg gcgttgttat tggtagtgcg
     2821 tttacaagca tcgtaaacca agtggtgcaa ggcttgatca caccactaat aggttggttt
     2881 gtcgcaatga tcacaggtac ttctgattta gaaggagcac tttcaattct tgactggtcg
     2941 ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta gtgctatcat tacatttctg
     3001 atcactggtt ttgtcttatt cttagttgtc aaagcggcaa accgtgcgaa gaatttacgc
     3061 tccaaagaag agcaagtgga agtggaagaa actcctactg cagaagatta tcttagtgac
     3121 atacgcgatc ttcttgccaa acaagtggca gaagacaata cacatccagt agagcaaaca
     3181 aatctttccg atacttctaa ataataaaga atctaaaaaa aagaaagccg attttcagtg
     3241 tcggctttct ttttttagat tcttaacttg ctacttgttc ctttttcatg tcgatatgtg
     3301 gaatatcatc ttccaaatac acatctgaga ttgccttgaa tccaaacgac tcataaaaag
     3361 cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc tggaaaattt ttctggatca
     3421 tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc tttacggttg tcctgatgaa
     3481 caaggacacg tccaaaatgg atactctccg cttcttcata tactcgcgtg taggcaagga
     3541 tcttcccatc agtatcttga aaaaacgtat gccaagcttg tccatcaatg tcatcaactt
     3601 cttgataagg acactgttgt tccacaacaa aaactgcgat tcgttctttg acgatcgcgt
     3661 aaaactctgc tgttgacaat tcttcaaatt tctttacctg atattccact aagcaaacgc
     3721 cccctgcttt tttattctta gcataccgat tcgcacacaa aaaagcaaac gaaccttacg
     3781 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
     3841 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
     3901 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
     3961 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
     4021 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
     4081 tttttaaaat tttcaagttt accataaaaa aaaaagaaat gcaataaaaa taaataagaa
     4141 gctcattaag atttttctaa attattcctc attataaaat ttcaagtgtt tttttatttt
     4201 gagttttact ctcccgtaag cgctcattac ttgcttgtga tccattgcta aacattcagc
     4261 catgtctaca aaatcaacgc cttgcatata ataatgaaaa attttagctt ctatttttga
     4321 aaactggatt tcgcaatttt cgattgcttc ttttaataaa agatgctgaa tcgctaaagg
     4381 ttcttgcaat gtgatctctg ctaaaaagtc tggtccttct gtctctattt tttggtcgat
     4441 tgagacttca tctttttgat cacgacgctt cagcgcgttc tgcttcctca acagagaata
     4501 gatttgattg cttataatgc ttttataata acttccaaag ctaacacctt tgcttttttc
     4561 gtaagttctc aatgcctgat ataaagcgat ccttgcttct tgttcccaat cctcagaaac
     4621 ataatttctt aaataatagg tgtttttcaa acgtagtaaa agtggaaaat atttttgata
     4681 cagggcagca aatgcttctt cgtcaccatt acgtgcagcg cgcaataact cctcagacat
     4741 aaaataattc ctccataaat tcgtttatgg aggaatctta tcatcaatga ttcatcgaac
     4801 tgattagaac gatatgttat ttattttttc tcattaaatc gtccaatttt tgggacaatt
     4861 tttttaactg ctcttcattc catggagaat tacgtcggaa atcaataaaa tgcatatctt
     4921 tattttcttg ggctattgtt ttttccgttt tttttatcgt cttatataat tcataagcag
     4981 aagttcgtaa tgctccttgg gaaaagatca tccactgttc tgctaaatcg cttgtcgcca
     5041 ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat tctctcgata taactgtcag
     5101 ccgtttcgtc ttctttcgta aaaacaacat ccaactggta tttcttataa ttttgttgga
     5161 tgcccggaac tagttgggca tcgaagacaa cgatgacttc cattccttca tatttcgcat
     5221 aatttgataa acgatgtaaa agagcttctc ttgcatccgc cattttgtcc tgctttttaa
     5281 gctggacaag ttccggccac gaaccaatca tgttgtagcc gtctacaatc aacagctgtt
     5341 ttttcatggt ctactccccc tgacgttggg aaaaagcctg atacataagc aaaccagcag
     5401 caacactggc atttaagctc tggacgtgcc cggtcatcgg gatcgtcagc aattcatcca
     5461 tttctttttt caatcctgga ctcattcctt tgccttcatt accaatgact aaagcaattg
     5521 atcctttggc attccatttt tggaagtttg tgccggtcat atctgtgcca aatacccaaa
     5581 atcctgtttc tttgagctgt tgcacagctt gtaccaaatt cgtcacgcgt gcaacaggga
     5641 catgttcgac tgccccagtg gaagtctttg ttacgatagg tgtgattcca actgctcggt
     5701 gttttggtat gatcacacca tcgacgccac ttgcgtctgc tgtccgtaaa atcgacccaa
     5761 aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa ggggtcttct tttttctctt
     5821 ttgttcgttc aatcagttgg tcaagggata aatattcata aggtgtaatc gcaagaacga
     5881 ctccttgatg cacgccgtga tcgctcaaag tttctaattt ttgtttagga acccatttga
     5941 ctggcacagc ttgttcacgt gcgatttctt tcagttcttc tactttttct cctcgtgaat
     6001 cttcttgaag aaagagttta ttccctcgcc cttgttgtag cgcttctact gcggcatggt
     6061 gcccaaagac gaaattatct tctaatgctt cttcattttg atcagaaact gactgatttc
     6121 gtccagtttg ctttttattt gaatatctat tttgtttttt gtcttcaaat ccacgtttcg
     6181 attgatgttt gtttgttctt tctttaccca tttttttcac caactttctg aatacaccag
     6241 cggatcaatt cttccatccg ctcggtttgt tttgtcaaat gcagatagcc catgagcgct
     6301 tcgaaccctg ttgcgacccg atacgtcgta acatcagcat tttttgcgct cgtatggctt
     6361 ttagcatttc ttcctctgcg ataataaagt tcttcttctt ctgtcaaaag atgttcttcc
     6421 agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa cataattcgt tgccgcacga
     6481 tgcagcttat tgggttttgt ttctccttga tcaatcaggt ggtcacgaac atagatttcg
     6541 taaatcgcat ctccaacata agccaaggct aaaccgttca attgtgtgta atctctcatg
     6601 cttctcttct ccatcgggtt ccttgcggtg tatcttctaa taagatccct ttttctttca
     6661 acaaatcacg aatttcgtcg cttcggacaa aatcacgatt gcttcttgct tgatttcgtt
     6721 cttcgatcaa ttgttcaatc tcttcatcta ataactcttc tgctgtaaat aaaataccga
     6781 agatcgaaag ccattgcgag aataaagctt ctccttgtgc caagacagcg gcagaaactt
     6841 tcggctggtc tagataagtg ttcagccact tagcaagctc atagaccact gtgatcccat
     6901 ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg ctgttctaaa ttttctaaca
     6961 aagcgatttt atccgcatca tctgccaaat tctctgaggc agtttcttgg cggaatttca
     7021 aattgtcaaa cgtattcttc agtcgttgta aattggtggc tgcttctttc atcgtcgttt
     7081 cactgtagcg gatcggacga cggtattgtg tggtcgccat gaagaaacgc aagacttgtg
     7141 gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt ccctagagat ttactcattt
     7201 tttcatcgtc ttcgccaatc gttacatagc cattgtgcat ccagtaatta gcgaattttt
     7261 tccctgtttt cgcttcactt tgtgcgattt cattttcatg atgaggaaat tctaaatctt
     7321 gcccgccacc atggatatcg atcgtttctc ctaaatgttt tgttgccata acggaacatt
     7381 caatatgcca accgggacgt cctttgcccc atggtgaatc ccaagaaatc tcgccaggtt
     7441 ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt ttgctgctgt tcttcccctg
     7501 tacgctgact tgctccgact tccagttcat cgatggattg gtcacttaat ttcccataat
     7561 tttcaaattt acgggtacgg taatacacat cacctgaaga ttcataagca tagccttttt
     7621 caaccaacac ttgaataaat gctaaaatat ctggcatatg atccatgact cttggatgca
     7681 gtgtagcagg ctcgacattc aacgctttcg tatcttcttc aaatgctcgg atgaaacgat
     7741 cagcaacttc tggtgctgta atacctaatt cgtttgctgt acggatgatt ttatcgtcta
     7801 catccgtgaa attcgatacg aaatcgactt tatagcctcg atattcaaaa tagcgtcgaa
     7861 ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg gatataatta tacacagtcg
     7921 gtccgcagac atacataccg acatgtccct tttgtatagg gatgaattct tctttttttc
     7981 tggtcaatgt attgtagatt tttatcattc tgatctcctc actttttctc ctttgattcg
     8041 aacgactttt gctggtactc ctaccgctgt agcgtcgtct ggaacatctt tcaacacaac
     8101 ggcacctgca ccgattttgg cgttttttcc gatcgttacc ggtcctaata tctgcgcatt
     8161 cgcagagagc atggctcctt gtttaacagt tgggtgtctt ttacccgttt cttttcctgt
     8221 gccgcctaat gttacgccat ggaataaaat cacatcgtcc tcgatttctg cggtttcgcc
     8281 aatgacgatt cccattccat ggtcgatgaa cacgccagtt ccaatcgttg ctcctggatg
     8341 gatctcaaca ccagttagga aacgccagaa ttgagcattg atcttcgcca ataaaaagag
     8401 ccggtgctga tataaaaaat gagagaaacg atgccaaaac aaggcatgaa aaccagggta
     8461 tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg tttctctttg ctgcatcaat
     8521 cgctcgggta aaccaactca tgactttcca ctcctttcgt aagaatttta tgtaggaaat
     8581 catcttctta taaagaaaag aagcgccttt taaataagtt tccttatcaa aagacgcttt
     8641 ccgcgtggtt ccacttttct tcataatcaa tgattatctc tgacagctaa cgcctgttat
     8701 acgttcttgg ctactttgtt tcctataaaa cagtttttcc tgttttctgt gtttcgccaa
     8761 gaccactccc agaggcattt cggcaaccat tcattgatac cttgcaccac ccggtatctc
     8821 tcttgtaatg atcagctgct tacttttttc tgttcatcgt gtttagttca gtacttgatt
     8881 taaatgtgct aatgattttt cacgaccaag caattcgatc gtttcaggca attctggacc
     8941 atgcatttgt cctgatacag ctacacggat tggcatgaaa agattttttc cttttacgcc
     9001 agtttctttt tgaacagctt tgatggcagc tttgacagtt ggagcatcta ctacttccat
     9061 ttgttctagc tgtgctttga atgcgtggag aacagtagga actgtttcac cagcaagtac
     9121 ttcttttgct gcttcattca atacaggatg ttcattgaaa aatagcgtag acagatcaac
     9181 gatttctgca gcatagctca tttgaggttg atacaggcta acgatcttct tcacccattc
     9241 gattgtcttt tcatctgggt tttctgctac acgtccatca gcgattagat aaggcagaca
     9301 catgtcagta aatgcatcta gatccattgc tttgatgtaa tggttgttta cccattgcag
     9361 ttttttcgca tcgaaagctg cgggtgattt acttaggcgt tttggatcga acattttgat
     9421 caattcttgg tgttcgaaga tttcgtcttc gcccacaggt gaccagccca ataatgcaat
     9481 aaagttgaac attgcatctg gtagatagcc taattcgcga tattgttcga taaattgcaa
     9541 gattgattcg tcacgtttgc ttagtttttt acctgtttca ctgttgatga tcaatgtcat
     9601 atgtccgaag gttggtggtg tccagccaaa tgcttcatag atcatcagtt gttttggtgt
     9661 attggcaata tggtcatctc cacgtaaaac gtgagtgatt ttcatcaaat gatcatcgac
     9721 cgcaacagca aaattataag ttggcatacc atcgcgtttt tgtatgacaa agtcgccacc
     9781 gatattatcg gattcgaacg aaatagcgcc tttcaccata tctgtaaatg cgtattctgt
     9841 attgcgaggc acacggaaac gtacaacaga ttctagacct tgtgcttctt tttctgcttg
     9901 ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga ggcatttctc ctcttgcgcg
     9961 ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa tagcatttgt atgctcgatt
    10021 gctagcaagc agctgatcaa tcagcggttg atagatttct ttacgttcag attgacggta
    10081 agggccgtat tctccaggat tttcaggaga ttcatcccaa ttcattccca accaagcaag
    10141 gttttccaac tggctttttt cgccatcttc gatattacgt ttttggtcag tatcctcaat
    10201 acgaataata aagtctccat cattgtggcg ggcaaacaaa taattgaata atgcagttct
    10261 tgcatttcca atatgcaggt gcccagttgg acttggtgca tagcgtacgc gtactttcgt
    10321 cattttcttc ctcttctttc aaactagact ttgactaaca gcaaaaattc cgtcagttac
    10381 ggcttctcaa atatcattta tttaaagcca ctcagcacaa attttacctt ttctaaggta
    10441 aatagtaaag ccaactggtt gaatttcctt accagttggc tgaatttctt ttttattttg
    10501 attgtttagt ctttttctct gtctcttttt tattgtcgat tcctcgtcct gcatgtgcag
    10561 gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc acttgttaca acgacttcga
    10621 tacgttcatt catgtaatgc tgtccatctt ctacgacaac catcgtgccg tcatcaaggt
    10681 aggctactcc ttgttggcgt tctgtcccgg cttttactac tagaacattc atcgtttcac
    10741 cagggatgac gactggtttt acggcatttg ccaacgcatt gatattaaga acaggtacat
    10801 tttggaattc agagacttta ttcaagttat agtcattggt tacaatgaca ccatctaaaa
    10861 gcttagccaa tttgatcaat ttgctgtcta cttcactgat atcttcaaag tctccatcgt
    10921 acatttcgac agatataccc tcttcttttt gcaaggcatt taggatatct aatccgcgac
    10981 gaccacgcac acgtttcaaa ctatcccctg agtccgcgat gtattgtagt tcgtaaagaa
    11041 caaaattagg gatcatcaag gttccttcta agaatccagt ctttgtaata tcataaatcc
    11101 gaccgtcaat gatcacgctt gtatccaaga ttttatattt atggaaattt tctcctgatt
    11161 ttcgttctaa cagttgactc tcaacttgac tagctgtttc ttgctcgatt tttttgcttc
    11221 gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt tcccattctc aatcccagat
    11281 agcctaagat gatcatcagt aaaatcggca atacactatt gacgaatgga atttccatat
    11341 tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc gatgatcgca ccgatcgcac
    11401 cgaagagaag ataagtcaag ctgacctcac ttagtttttg ttctacttgt ttgataatag
    11461 ctgagatatg tttggccaac agcaaagata ataaaaagaa aataagtgcg cctaacaggc
    11521 tgttggtaaa tgtattattc agccatccgt ttgactgttg accgattgcc tgccaagctg
    11581 agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat catcagcaaa gtaatgacac
    11641 gtttttgcat cgtttttcct ccgtttcgac aatcatttat tagttagcgg aacacacgct
    11701 tcaacgtttc agccaaagtt gctactccaa ctatctcgat tccttttggc gcttcccagt
    11761 tacctaagtt gtttttaggt aggtatactt ttgtgaagcc gagtttttgg acttctttga
    11821 ctcgttgttc aatatgactg acacgtctga tttctccagt cagtccaatt tcaccgatga
    11881 aacattccgt tggctgtgtt cctttttctt tatagcttga agcaatactg acagcgatag
    11941 ctaaatcaat cgctggttca tttaatttta ccccacctgc agcttttaag taagcatctt
    12001 gattctgaag aagcaaccca gctctttttt ccagcactgc catgatcaga gatacccgat
    12061 tgaaatcaag acctgttgtt gttcgtttgg catttccgaa catagtaggt gtgactaatg
    12121 cttggatctc taccaaaatt ggacgtgtcc cttccattgc cacaacgata gcagagccag
    12181 ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg attagcgact tcttccaatc
    12241 catgctcatg catttcaaaa atcccaatct cattcgttga gccaaatcgg ttttttactg
    12301 ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa atataacacc gtatccacca
    12361 tatgttctaa cattcttggg ccggcgatcg agccttcttt ggtaacatga ccgacgataa
    12421 aaatggctat cccatttgtt ttagcgattt tgagtagttc tgcagtggtt tctcttactt
    12481 ggctgacact tcctgccaca cttgtgatgt ctggctgcgt catcgtttgg atggaatcaa
    12541 tgataacata atcaggggag atatgttcga tggcacgact gatttcgttc atatctgttt
    12601 cagcgtatag ataaaattct gtgttgatac ttccgaggcg ttccgcgcgt aattttatct
    12661 gctctgcgct ttcttcgccg gatacatata agacttttcc gcctatggct gctaattgct
    12721 gagaaacttg taacagcaaa gtggatttcc cgatacctgg atcgccacca atcaagacca
    12781 tagaccctgg caccacacct ccgcctaaca cacggttcag ttcttctaat tttgttttga
    12841 tccgaggttc ttttttaggg acgacgtctg cgatttttgt tggttttgct ttttctcctg
    12901 tcaaacttgt ccgagttctg cggtccgttg tatcttgttc gatttcttcg accatcgtat
    12961 tccattttcc gcaatttggg cagcgtccca agaacttagg cgacacatac ccgcaatttt
    13021 ggcaaacaaa ttgtactttt gatttttttg ccattgtacc gcctcctttt atcctaatca
    13081 tgagacatta aatcaagctt aagtatcttt aacgttttat ttactggaag aaccaaatcc
    13141 gccttgacgt tgagcagtcg tttgatcttg atctgctttt aaaaacggca aaaagatccc
    13201 ttggccgata cgctcgcctt ttttgatcac tacatccttg aaaccaaagt tggtgaattg
    13261 gaacatgata tggccttcat ttgcctcatt attgtaataa tcactgtcaa tcacacctac
    13321 tccgtttgtc aacactaaaa accgcttcaa cggattgctt gaacggttag caagctgcaa
    13381 aaattcatct tctcccatgt aagctttgat tccagtaggc acgagggttg gtttcaattg
    13441 tttttcattt tctgatacat cttttttcaa tccatctgcg actgtttcca accccttttt
    13501 ccagcctgct ttccagatac ttggaatcac gacctcttct gctgcttcaa aatcatagcc
    13561 agcagcatga tgtgtagcgc gctgcggtag attgatattt tgatctttat atttagaaat
    13621 aacttcaaat cctctttttc tcaccggttt tcctccctaa ataaactact cctattctaa
    13681 catatccgag gctgtaaaag actttcttta caaattcttc aagaaattct gctatcttaa
    13741 agtaaaccat taattcgttc tttttccaaa cgactgcaag ttgtacaaaa aaactatttt
    13801 ttacatgact ttactcgtca aaacaagtag aatagaacga aattaatgag tggatatttg
    13861 attgaggtgt cacatgcgta aagaaattaa aaatgcaaaa cgaattattg tcaaagtagg
    13921 aacaagtaca ttgatttatc ccaatggaaa tatcaatctt gcggcaatcg atcagttagc
    13981 ttttgtcctt tctgatctta gaaatcgggg aaaggaagtg atcttagtct catcaggagc
    14041 tgtaggcgtt ggtatgcata aattacattt gtcagaacgt ccgactttga ttcctgaaca
    14101 gcaagctgcg gcggctgttg gtcaagccga actgatgaat atctataatc aaagattcag
    14161 tgtatataat caacagacgg cacagatcct cgtgacaaga gacgtggtag attatcctga
    14221 aagtcgtaaa aatgtgagga atacattgga acagcttttg attatggaca cgatccctat
    14281 cgtgaacgaa aacgatactg tagcagtgga tgaattagat catctgacaa aatttggcga
    14341 taatgatcag ctgtcagcca tcatggcaaa acttgtcgcg gcagaccttt tgatcgtcct
    14401 ttctgatatc gacgggtttt attcagataa tccgactaca aatcccgagg ctcagatgta
    14461 tcgctatatc gataaaatcg atgaaaagat tctggcacaa gcaggtggtg ctggtagcac
    14521 atttggaaca ggcggtatgc agagtaaatt aaaagccgct tctaggatat ttgaagcaaa
    14581 cagcgcaatg attttagcaa atggaaaaaa tccggcaatt atttttgata ttcttgcagg
    14641 aaaagaagta ggtactttat ttaaggagga cttgaaatga ctgacttgat gcaattagga
    14701 caacaggcaa aagatagcgc gcaacaacta gcacttatgc ctacaaaccg caaaaatgag
    14761 ctacttgttc aaatggcaca aaccatcaaa aagaatcaac aagcgatcat tgaagcaaat
    14821 cataaagact tagaaaaagc agtcgaaaac aatatcagtg aaacgatgca agaccgcttg
    14881 cagctgacac ctgagcgaat caatgccatg gctacagaga tcgaaaaaat tgcttcgtta
    14941 gacgatccta ttggaaaagt agatgagatg tggacgaaca ccgacggatt gagaatcggc
    15001 aaaaagcgcg tgccactagg cgtgatcggc atcatttacg aatctcgtcc aaatgtcacg
    15061 acagatgcag ctagtctagc attcaaatca gggaatgccg ttatccttcg cggtggcaaa
    15121 gaagcattct tctcgaatca attattagtc caattattgc agcaagtact ggtcagtgct
    15181 ggcgaatctc cttatgccat ccagtttgtc gatgatactt ctcatgaaac tgcaggacag
    15241 ttgatgcgat tgacagaata cttagatgtg ctgattcctc gaggcggagc aaaattaatc
    15301 caacgagtca aagaacaggc aacggtacct atcattgaaa caggtacagg aaataatcac
    15361 gtatatatcg ataaagaagc acagttatct atggcagtga acatcatcgt caatgcaaaa
    15421 gcttcccgtc catctgtctg taacgcggca gaaacattgc tgatccattc tgagatcgca
    15481 ccttttttct tgccagctat tgaaaaagaa ctggtagaac acggggtctc tttacgtgcg
    15541 gatgctcgtg cgttagaata tctagagacg gctgctttag cagaagacac agattgggat
    15601 actgaatatc tagattatat ccttgctgtc aaagtggtcg attctttgga tgaagcaatt
    15661 gcacatatca atcggcataa tactaaacat tcagaaacga tcgtgacaga tagttatgcg
    15721 gctagtcaac gtttcttaaa tgaagtggac gcggcggctg tctatgtcaa tgcctccact
    15781 cgttttacag acggatcggt atttggtttt ggtgcagaga ttggtatctc tactcaaaag
    15841 ttgcatgccc gtggtccaat ggggttaaat gaattaactt ccacaaaata tatcatttat
    15901 ggcgatggac aaatccgcga ataatgtttc gtgtgaaaca ttattcccgc cttttgcctt
    15961 ttttctgtaa aggttctatc caactagaaa ggtgtcaata tgtttgaaat tttaatgatt
    16021 ttttttattt accttatttc tctgaatatc gctgcttttc tgggagtcgg tattttatcc
    16081 cttttctttc aattcaaaaa acgaagcata gggagtcaac gggaaaaatg gtcgcagtat
    16141 tttgacaaaa tcgggcaaaa agggctggtt acacgcttgc acattagtta catggttgca
    16201 ttatgcttat tagcgacaat caattactat tcttttttcg atcattcaat cgcttatacg
    16261 attacgctac tgattgctgg gatttttcac ttatcttata aatatcagct gaataaaaac
    16321 cacttgaatc ggactttccg ctaaaaaata tccgtacgat aagtagagtg ccttgaagat
    16381 cggcaaaaaa ctgccggact ttttagaggc tgtgacaaag attttgtcac agcctctttt
    16441 tccatataaa cggcgttcgc ctaccagctg cctggtaaat ctgtttcctc ttcgttaaga
    16501 agcgaaaatc cgtcaaatgt ttcgccatca taataacgaa gcagctcatc catggacaag
    16561 ttcatcgttt tttctttttc ttcttccgct gattcagcca gtagctgttc ccgatacacc
    16621 tgatacatac cagctatttt ttgctgcatc gcttttctag tttcaccttc cgcattgatt
    16681 attggcaatt ctttgatata gccacgataa atcaaccgat catctgaaaa ttcaatgggt
    16741 aattcaacga gtgtcaaccc gacttcctga aaatattctt ccagcatcta ttatcccccc
    16801 attctttttc ttaaatgaat tttagcttac ctactgaaaa ttttccagtt tcattatttt
    16861 gagaaaaaaa gggaatgtga caaaaatcgt gtcacatccc ctttttccga ataaatggtg
    16921 ttcttctgcc aactcctcaa tcataagtca caatattcaa caaacatgag aaactgtttt
    16981 ttgaaattgt tccttgtgat tcagagttaa acgacagtct cacaacctct tggtaaacgg
//
LOCUS       DACEMO010000041        16674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEMO010000041 DACEMO010000000
VERSION     DACEMO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.042"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW728_001046"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW728_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1024420.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IW728_001047"
     CDS             808..1242
                     /locus_tag="IW728_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1024421.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IW728_001048"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IW728_001048"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1024422.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IW728_001049"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IW728_001049"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1024423.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="IW728_001050"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="IW728_001050"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1024424.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="IW728_001051"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="IW728_001051"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1024425.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="IW728_001052"
     CDS             9513..10439
                     /locus_tag="IW728_001052"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1024426.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="IW728_001053"
     CDS             10621..11514
                     /locus_tag="IW728_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1024427.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="IW728_001054"
     CDS             11648..11773
                     /locus_tag="IW728_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1024428.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="IW728_001055"
     CDS             complement(11907..12539)
                     /locus_tag="IW728_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024429.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="IW728_001056"
     CDS             complement(12687..13229)
                     /locus_tag="IW728_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1024430.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="IW728_001057"
     CDS             13364..13753
                     /locus_tag="IW728_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024431.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="IW728_001058"
     CDS             13757..14521
                     /locus_tag="IW728_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1024432.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15597
                     /locus_tag="IW728_001059"
     CDS             14650..15597
                     /locus_tag="IW728_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1024433.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15727..16467
                     /locus_tag="IW728_001060"
     CDS             15727..16467
                     /locus_tag="IW728_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024434.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         5887 a         2819 c         3457 g         4511 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaaacgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct
    15181 tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt
    15241 aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg
    15301 gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca
    15361 gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt
    15421 gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt
    15481 atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg
    15541 agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaattaagtc
    15601 ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa tcagcgcatt
    15661 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
    15721 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
    15781 ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga agatcttttg
    15841 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
    15901 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
    15961 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
    16021 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
    16081 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
    16141 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
    16201 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
    16261 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
    16321 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
    16381 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
    16441 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
    16501 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta tatcgtatcc
    16561 tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa taatggcaaa
    16621 ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt ttta
//
LOCUS       DACEMO010000042        16509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEMO010000042 DACEMO010000000
VERSION     DACEMO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.043"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            193..1131
                     /locus_tag="IW728_001061"
     CDS             193..1131
                     /locus_tag="IW728_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1024435.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            1146..1976
                     /locus_tag="IW728_001062"
     CDS             1146..1976
                     /locus_tag="IW728_001062"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1024436.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            2020..3045
                     /locus_tag="IW728_001063"
     CDS             2020..3045
                     /locus_tag="IW728_001063"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1024437.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            3060..3998
                     /gene="galU"
                     /locus_tag="IW728_001064"
     CDS             3060..3998
                     /gene="galU"
                     /locus_tag="IW728_001064"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1024438.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            4124..4564
                     /locus_tag="IW728_001065"
     CDS             4124..4564
                     /locus_tag="IW728_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1024439.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            4567..5169
                     /locus_tag="IW728_001066"
     CDS             4567..5169
                     /locus_tag="IW728_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1024440.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(5231..6334)
                     /locus_tag="IW728_001067"
     CDS             complement(5231..6334)
                     /locus_tag="IW728_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1024441.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            6544..7545
                     /gene="ccpA"
                     /locus_tag="IW728_001068"
     CDS             6544..7545
                     /gene="ccpA"
                     /locus_tag="IW728_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1024442.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            7899..8294
                     /locus_tag="IW728_001069"
     CDS             7899..8294
                     /locus_tag="IW728_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024443.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            8291..8545
                     /locus_tag="IW728_001070"
     CDS             8291..8545
                     /locus_tag="IW728_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024444.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            8588..8983
                     /locus_tag="IW728_001071"
     CDS             8588..8983
                     /locus_tag="IW728_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024445.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            8983..10038
                     /locus_tag="IW728_001072"
     CDS             8983..10038
                     /locus_tag="IW728_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ1024446.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            10035..10241
                     /locus_tag="IW728_001073"
     CDS             10035..10241
                     /locus_tag="IW728_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024447.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            10253..10576
                     /locus_tag="IW728_001074"
     CDS             10253..10576
                     /locus_tag="IW728_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024448.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            10569..10835
                     /locus_tag="IW728_001075"
     CDS             10569..10835
                     /locus_tag="IW728_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024449.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(10998..13343)
                     /locus_tag="IW728_001076"
     CDS             complement(10998..13343)
                     /locus_tag="IW728_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1024450.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            13622..14524
                     /locus_tag="IW728_001077"
     CDS             13622..14524
                     /locus_tag="IW728_001077"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1024451.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            14735..>16509
                     /locus_tag="IW728_001078"
     CDS             14735..>16509
                     /locus_tag="IW728_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1024452.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         5451 a         2955 c         3453 g         4650 t
ORIGIN      
        1 caatcgaaat atttttgagc tatgtacaag aagcatttta ttctagctta atttgacaaa
       61 tcatatataa ttaagaatgc gttttctatt acattggtaa ttaaataaaa acgttttttt
      121 taattggtag tacttttggc tagacaatga taaaatggta gagaacactc taatagaaat
      181 gaggtagcat gaatgtcaga agttgtaaaa gttagccaat tagtaaaaaa attatctttg
      241 gaaatcgtca caggcgacga gaaaagttta gaccgcgtca tcgtgactgg ggacatctca
      301 cgcccaggac tagagttgac cggttatttt aattattatt ctcataatcg tttacagcta
      361 tttggcagta aagaaatcag ttttgcggaa cggatgatcc cagaagagcg tttgatcatc
      421 atgagaagat tatgtagtga agatatgccc gcatttatta tttcaagagg attgccggct
      481 ccggaagaaa tgatccaagc agccaatgaa aatgggatag ctgtgctgcg ttcgcctatc
      541 tcaacgtctc ggctgctagg tgaattatct agttatttag atgggcgctt agcccctcga
      601 acaagtgtcc acggtgtgtt agtcgatgtc tacggattag gtgtactaat tcaaggggac
      661 agtgggatag ggaaaagtga aacggcgttg gaactgatta agcgcggaca tcgattgatc
      721 gcagacgatc gagtagatgt ctatcagcaa gatgaactga cggtaatcgg agaacctcct
      781 aaaatcctgg aacatctgat tgagattcga gggatcggga tcatagatgt aatgaatctt
      841 ttcggtgcga gtgcggtacg aggctctatg caagtccagt tagctgttta tctagaagca
      901 tgggcgaaag ataagaaata cgaccgtttg ggttctgaag acacgaccgt tgaaatcgcg
      961 gaagtgggca ttccacaaat aaaaatccct gtcaaaacag gacgaaacgt tgcaatcatc
     1021 attgaagtag ccgcgatgaa tttccgcgcg cgtacaatgg gctttgacgc aacgaaaaca
     1081 tttgaagaaa gattaagtcg cttgattgaa gaaaactcag gcaatgacta gcaaggagtg
     1141 acaatatgtt agcacaaatc aatcgagttg cttttgagct tgtaggaatt cctatctact
     1201 ggtatgcatt gattatcgta tcaggaatca ttattgccat gtggctcagc agcagagaag
     1261 ctgttcgggt aggattaaaa gcagatgacg tgacagattt tatgttgtgg ggattgccga
     1321 tttccattat tggtgcccgt ttgtattatg ttttattcga tcttccccaa tatatagctg
     1381 atccaatcca aatattcaat atccgttcag gaggattagc gatttacggg ggattgattg
     1441 caggtgcaat cacactgtac ttttttacca aatatcattt tatttctttg tggacctttt
     1501 tagatatcgc tgcaccaagt gtattactgg cgcaggcaat cggacgatgg ggaaatttca
     1561 tgaaccacga agcgtatggt ccagacacga cacgaagttt cttagaaagt ctgcatttac
     1621 cgcaattcat cattgataac atgtatatcg aagggatcta tcgacaacca acctttcttt
     1681 atgaatcact ttggagtttg attggattta ttctgctttt ggttttgcgg aagaaaccgc
     1741 atcttttgaa aaaaggagaa gtccttttat gttatctaat gtggtatagt ttcggacgtt
     1801 tctttatcga aggattacgc acagacagcc tttacttttt aggtgtgatc cgcatttctc
     1861 aattattgtc tgctgtttta cttgtcggca cattgattct gtttatttgg cgtagaaaaa
     1921 aaggaaattt accggattat gaccgctcat taggaaaaaa tcaacaaatt atttgatttt
     1981 tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa tgaattatag agtagcggtt
     2041 ttaggaccag gctcttgggg gactgcttta gcacaagtcc tcgctgaaaa cggacatgac
     2101 gttcggattt ggggacatcg tgcttcacaa gtagaagaaa tcaacaccca acacaccaat
     2161 caacgttatt taccagatca tcatctaccc gattccatcc gagcattcac cgatatggaa
     2221 gatgcagtaa aagatgcaga tgcggtacta ttcgttatcc cgacgaaagc gatacgttct
     2281 gttgcaaaag aactagttgc caaattgaat acgaagccag tgatcatcca cgcaagtaag
     2341 ggattagaac agggaacgca taaaagaatc tctgaaatct tgttagagga aatcccgaca
     2401 gaaaaaagaa aagctgttgc tgttctttct gggccaagcc atgcggaaga agtagcaaag
     2461 catgacatta cgacgatcac agcggcaagc acagatgcaa aagcagcaaa atatgtacaa
     2521 gagctgttca tgaaccatta ttttcgaatc tatactaacc ctgatgtggt tggtgtagag
     2581 atgggggcag ctttgaaaaa tattattgcc attggagctg gtgctattca cggcttaggg
     2641 tttggcgata atgcaaaagc ggcaatcatg acgcgcggat tagctgagat cagccgttta
     2701 ggtgtagcaa tgggtgccaa cccgctgacc tttattggct taagcggagt aggcgatcta
     2761 gtcgttactt gcacaagtgt tcattccaga aactggcgcg caggcaactt gttaggacaa
     2821 ggacagcctc tagaagaggt gctagccaat atgggaatgg tcgttgaagg tgtgaatact
     2881 actcaagcag cgatggaact agcccaaatg atggaaatcg aaatgcccat cactcaaaca
     2941 atctatgaag tgctgtatga acaaaaagaa atcaaaacag ccgcaaaaga aatcatgtta
     3001 cgtgatggga aaaaagaaaa tgaattctca ttaacaattt tttgaaggag cgaatgtata
     3061 tgaaagtaaa aaaagcagtt attccagcag caggattagg aacaagattt ttaccagcaa
     3121 caaaggcgat ggcaaaggaa atgttgccaa tcgtggacaa accaacgatc caattcatcg
     3181 ttgaagaagc gttgaaatca ggaatcgaag acatcttgat cgttacagga aaagctaaac
     3241 gaccaatcga agatcacttt gattcaaata tcgaattaga aacaaaccta agtgaaaaag
     3301 gaaaaatcga attattgaaa ttagtagaag aaacgacaga tgtcaacttg catttcatcc
     3361 gtcaatctca tccaaagggg ttaggacacg ccgttcttca agcaagagcc tttattggaa
     3421 atgaaccgtt tgtcgttatg ctaggcgatg atatcatgga agacgaagta ccattgacac
     3481 agcaattgat cgatgactat gaacgtacac acgcttctac gattgccgtt atgcaagttc
     3541 ctcacgatga aacttcaaaa tacggaatta tcaacccagg ggaagtactg gaagatgggt
     3601 tatacaatgt caaaaacttt gttgaaaaac cagatccaag caaagcacca agtgatttag
     3661 ctattattgg gcgttactta ttgactccag aaatctttga tgtattatca gaacaacaac
     3721 caggtgctgg aaatgagatc caactaactg acgcaatcga tacgttgaat aaaacacaac
     3781 gcgtatttgc ccgtaaattc acaggtaaac gttatgacgt tggtgacaaa tttggtttta
     3841 tgaaaacaag tattgaatat ggtctgacac atccagaagt aggggcacct ttgcgtgaat
     3901 acatcatgtc tttaggtaaa acattagaag cagaaggaag caagatcgat cataatccaa
     3961 aaggaataac agaaatcaaa gaagaaaaga aaaaataatc atttttttct aacgagcttc
     4021 agctgctaat tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa
     4081 atgctatcct tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat
     4141 tgcaggattg atcgcagcaa tcgcctttgc gatacttgtc gttttcatca ttcgcgtatt
     4201 gttgcaagtt tccaaaactt tagaaaaagt agaccagact gtcgcagaag caaatacgac
     4261 aattgagatc gtcacaaaag acgtagatct tttatctaga caagtagaag gcttgctggt
     4321 caaaagtaat gagctgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt
     4381 cacagcagtt gctgatttaa gtgaaagtgt atcggaactt aatcattcta gtaaaaacat
     4441 cgtgacgaaa gtcggcggaa tcggaaaaac gacagctaaa gcgactgttg ccggaaaagt
     4501 cggtggaaca gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa
     4561 ataagaatgg ctaaaaaagg tggattttta ttaggtgcag tagttggagg tactgccgca
     4621 gcaatcgccg cattattgtt agcacccaaa tctggtaaag aacttcggga tgaattggct
     4681 gctcaagcag atgatttgat ggacatggct tcagattaca cagatatggc aaaagaaaaa
     4741 tcagctgagt taacaggcat tgcaaaagaa aaagcgcaag atctttctca acaagcaggg
     4801 gatctagcag gtaatgttaa gcaaaaggta agtgaatcag cagaggaagc aggaagtgaa
     4861 tcagatgata tcttgttaaa cctcaaagaa caatcagctg atttatctaa tcgtttcaaa
     4921 aaaacagcag aagatctgaa aaaaccagca gacgaattag gtagtattgc taaagatact
     4981 tcagaagata tcatgatcga tgtaaaagat gctgcagata aagcaggaga gacggtcaat
     5041 gaaggaatgt cagaagcaaa accagttgca gatgacacaa aagaaatgtt gaaaatggca
     5101 aaagaagatg caaaagaagc taaaaaagaa atgaaagata caatgacaga ttcgactacc
     5161 aatcactaag aatactcatt tctagaataa aaaggtgaga gcggaccagc tcccaccttt
     5221 tttattttca ttactctaaa acaagtaatt cttttggtgt agtcgtgaat ggcacacaac
     5281 catcacttgt cacatacaca caatcttcga tacgtacccc tactttttca ggaatataga
     5341 ttcctggttc aagagagaag cacatccctt cttcgatcac taagtcgttt ccttctacaa
     5401 gagaagggaa ttcgtggacg gttgtcccaa ttccgtgacc taaacggtga ttgaaatatt
     5461 cgccgtagcc atagctgttg attacgtttc gtgcgatttg atcaagttca cctgctgtca
     5521 cacctgggcg aactgcctct tgtgcggcaa gctgtgcttc aagagtgatg ttgtagattt
     5581 tttcttggaa ttctgttggt ttttgataag caactgtacg agtagcatca ctgcaatagc
     5641 cattccaaac aacgccaaga tcgaataaca ctagttcatt tggtgaaacc ttcgtgcttc
     5701 ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaaggtg tcgaaagaca
     5761 tttgtgaaac accttgtttt tttaattggt attcgatttc tgcgataatt gcttgttcag
     5821 tcacgccttc ttttactgtt ttgaagccaa tttcaaaggc aacatcggcc catttaccag
     5881 cttcgatcag tttgtctttt tcttctaccg tcttgatcag ttgcagacgc tggataatag
     5941 aagttagatc agtgtaagaa tctgcacctg tcaagatatc agagatagca tcgaagcgat
     6001 cgacagtcag agcattcttt tcaatcccta cattaccagg ttgcccatag cgtgcgcgaa
     6061 gctcttttcc gatgatctcc caagggtttt cgctgtctaa gtagccacgt acatcgtacg
     6121 cccagctgct gttttgagca tcttcaactt ccagagcagg agtgaaaagg aaaggatctt
     6181 gttcaagagg gataaaaaga gctagtacac gctcgtgagg atcactttca tatccggaaa
     6241 agtaagcgat gtggccaggg tcgctgatat aagccaattc tacttggtgt tgtgccatcc
     6301 attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc taaattaatt
     6361 ttgtattcaa taattgtacc actgattgat taaaagacca acgttcttaa aactgaaaaa
     6421 agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttgttgagat tatttgatat
     6481 cctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa taggagaaca
     6541 caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa tgtttcgatg
     6601 gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac aagaaaaaaa
     6661 gtcttggaag ttatcgatcg cttggattat cgtccaaatg cagtcgcacg tggcttggca
     6721 agtaagaaaa caacaactgt tggggtaatc attcctgatg tcagcaatat gttctttgcc
     6781 tctttagcac gtggtatcga cgatgtagca acaatgtaca aatacaatat catcttagca
     6841 aactcagatg gcaacgatca aaaagaagtc aatgtattga ataatttatt ggctaagcaa
     6901 gtggacggcg tgatctttat gggtcaccat atcacagacg aaatccgtgg agaattttca
     6961 cgttcaaaaa cacctgttgt actagctggt tccattgatc cagatgaaca agtaggtagc
     7021 gtaaacattg attatgtatc agcaacaaaa gatgcagtaa ataaattagc aaaaagcggc
     7081 aataaaaaaa ttgcttttgt cagcggtgcg ctgattgatc ctatcaatgg tcaaaaccgt
     7141 ctaaaaggct ataaagaagc attaagcgaa aacaatctac catatagcga aggattaatc
     7201 tttgaggcac aatacaactt taaagatggt ctgtctctag ttgaccggat ccataatagc
     7261 ggtgcaacag ctgtctatgt ttcagacgat gaattagcca ttggtatctt ggatggttta
     7321 ttagaccgtg gcgtaaaagt accagaagaa ttcgaaatca tcacaagcaa caattcttta
     7381 ctgacagaag tagctcgtcc tcgtttgaca agtatcacac aaccattata cgatttagga
     7441 gcggtatcca tgcgcctatt gacgaaaatg atgaataaag aagaagtcga agaaaaaacg
     7501 attatcttgc cttatggaat cgaagaaaaa ggatcaacta aataataaaa gatcaatcat
     7561 ttagaggttg ttccaaaagt ggccaacttc tggaacaacg tttattcgag aaaaggggtt
     7621 atgacaaaat tttgtcataa cccctttttt ttgtgacttt aaaaaaagag aaaatggcgg
     7681 aaacaaacct tacgtccatc gtatacgaca caatctgtgc gtagatgcta cattttgttc
     7741 actactatca aaattacttt aaaatgaaaa cgtgataaaa aagaaagtag aaaagtagaa
     7801 aagtaactct aaaaaatcca aaaaaatcca ggctcgattg cgtacttatc ttaaaggaga
     7861 aacaatccat catacataac ccgaaaggag acttctattt gcctaagaaa agacgaaaga
     7921 ccatagtcat cattttgacc attcttgcta taggaatcgg aggaaaattt tacatggaca
     7981 aaagagaagc acaaagacag aaagatttac tcgcagtaga aaaacaatcc gtcaaagttc
     8041 tgaaaaatac ctttgcagat atcagggaag tgaaggtaga agaatttaaa aaaaaccctg
     8101 ttactggatg ttatggtgca cttgtgacaa tgacaaataa tgaagggaaa tctgtttatt
     8161 ttgattattt tttttcaaaa caaatgaatg aaattagcag ttatggtgta gaaaatgaag
     8221 aaatacaaaa cgaaggggtt actactccta aaataagagt tatttattct aatggagagg
     8281 cggaggaagt gtgacagtaa aagacgaaga gtatagatat ataagtgaac atgtttattg
     8341 gctagatcca aaacatcctc aatatagtaa aagttacaaa gaaggcgcca ttaaaaactt
     8401 tggagaaaca aaattccaaa tcctaaaaat caaagactca ttggatggca tgcaggcgat
     8461 ggctgcagcg ccaatcgtcc atagcaaatt ggagaaaaat ttcaaaaata aaaaaattcc
     8521 tacaaacttt cgcgtactta aataatgaag gtatcaatac ttacataaaa agaaaggagg
     8581 cacgttcttg tctcggaaaa taagaaaact cagcttagcc attttattaa tcgtcatatt
     8641 aggaatcgga gggaaagtct atatggacaa aagagaagca caaagacaga aagatttact
     8701 cgcagtagaa aaacagtcag tcaaagtttt gaaaaatact tttgcggata tcaaggaagt
     8761 gaagattgaa aaatctgctc gaaatgaaat gacgggagca tatcgtatgt ttgtgacaat
     8821 gataaataat cacgatgagt ctgtttattt tacgtttgga ttttggaaag aacgaaatga
     8881 attaggagat tatggcgtag aaaataaaag tatacaaaaa gaggggatta cagtaaataa
     8941 aatcagagta attttttcta ataataagga ggaatatatt taatgatggc agacaaacaa
     9001 tacagtgtta ttagtgaggc tgtttactgg ctagatccta aacacagaga ctatgatcat
     9061 acttataaag aaaattctat taaaaaaatt gatagttata attttaaaat cctaaaaatc
     9121 aaagactcat tggacggcat gcaggcgatg ggtgtagcgc caatcaagaa cggtaaagtg
     9181 gatctttctc aagtagtgat tgcttttgca ggaacgaact taagtgattg gaaagatttg
     9241 gaaacggatg caaggagtat catgaatctc tatgggaata gaccaggagc aagtttttct
     9301 gaaaaagcaa caacaaacaa gattttatcc gttagtggat tgaaaacagc ggggcaaatc
     9361 aattcagcga ttgcatttgc taatgaagta aaaaaggaat atggagaagc tgctatttct
     9421 actaccggcc attcattagg tggattctta gcattatatg tagcagcaga gaaacaatgg
     9481 aaaaatgtcg gcttaaacgg gccggacccg tatgatctct tatcaccaga agcaaaaaaa
     9541 tgggtggaag aaaatcccgg tatgctcacc aattaccgaa atcacgccga ttttatcggt
     9601 aattttggtg gtaatggcac aggtgctgaa gttcggatca gtatggaaac agcattcttg
     9661 aatctgcttg cttcccacag tttaacctct tggaagtttg atgaaaatgg aaagctgatt
     9721 attcctgaaa atgaaaataa tgttaaagct cgaaaacaac agaaagaaaa ccaagcgacc
     9781 cagttgtttt cctatcagtt gcaagaactg aacaacatac gtaaaaaact acgattaagt
     9841 ggcggccggc tgacagttaa tgaacaaatt tacttagatg acacacaagc taggttagta
     9901 gtggaaaagt tatcagcaaa aatgaaaagc gctatggaag aagttatccg catcaatcaa
     9961 agaggaattt ttgagctaga gaaaaaatgg catggcaaga aggcttgcgg caagcacgtg
    10021 ttgtcgcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt gttgtcgcca
    10081 caaaagagaa cttagtcgat tctgcgaaag aagtctatct gctgcgaatc aataaggcaa
    10141 gacgtatggg ggaggccttt gatacattgg tgaaggagat ccaagacaaa atcaatgaaa
    10201 tcgtgacacg agatcgagaa ttagcacagc aattcaatta aaggagaagt agatgaaact
    10261 agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc aagaagatga
    10321 attgatccac tttaagaatc aaggattacg attgatggag caagaggagc agcgactgca
    10381 tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa gacgaagtta
    10441 ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa aactctatca
    10501 gcagcaagac gaactagaac gttcctatcg tcaaaaaatc agacaaataa ctatggagaa
    10561 atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa aatctacatc
    10621 gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca gaaaaacgat
    10681 tgacagatca aaaccaccaa atgactgcag aagaagctgc tcaagaattt tctaaccgac
    10741 atgtacactt gaaagaatca gtaaaagggc aatttgcaga aaaactaacc gaattattcc
    10801 aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa aaattttgtg
    10861 aaaatggctt ttcacatttt tacataaatt tgtcttaata tcgtagatgc aagcttttga
    10921 acaccattta ttcggaaaaa gggagctgtg acaagatttt tgtcgcagct ccctttaatt
    10981 ctgattaatc tattagatca atcatcttta tcgtctgatt ctttggtctc tggattcgct
    11041 ctagcataag ctgaagcggt gttccagtaa tctgtatagt tttcatcagt accgccaacg
    11101 ccgaatttaa aggcgctttt cttcggacca ttttttgccc aaagtgattc cactgttttt
    11161 cctggcgttt ggatagattt tttattgaca gtaacttttc cagctgtttg tcctgtgaac
    11221 ccagatactt ttttcttcat cacatcagat gataagtcaa atttctcatc agtgccgaaa
    11281 atctcagggt ttgcttggta agcttgattg attagattgg caagataatt agccgaacgt
    11341 atccttgctt ggctgtccat tccagtattg tcatcgtgtc ctgcccagct actgatagtg
    11401 atagacggag tagagacgat cagccatgaa tctctgtatt cgttggtaga accagttttt
    11461 ccgacccaat ctgcttttcc tagatttccg tttaagcttg aaatgacatc tttgaatggt
    11521 gtagtgattt tttcattgat cacactgcgc atcatgtcat tcatgatcga ggccgttgct
    11581 tcggaataaa tacggacaga attactttca tgtttataaa tcacattacc tgcattatct
    11641 gtgatggaat caatgatata gccttcttcg tagacgccac cattagctaa ggcctggaat
    11701 ccatttgtct gttgaagcgt cgttacatct actgtcccta aaggagcaga ttcaacgccc
    11761 caattatcat ttgctgggta gttcatcttc gccaagtagt gctcgtaagc atattgtttc
    11821 gatccacccg aattcaatac gtcttggtat aagtgataag ctgggatatt gttggaccat
    11881 tccagtgact cacggacggt ttggaaggtg ttcgaccctt cattggtcgc attgacgatt
    11941 ttctcgccag cattttctcc ttcttgccaa gtcgttgcat aatctgatac acgggattct
    12001 gatccgacaa gtcccatatc gattgcagga ccgtaaacaa gaaccggttt gatggatgaa
    12061 ccagcttgac gttctgtatc aaaggcgtgg ttattttgat tctggctgta attccggcca
    12121 cccacgaatc catagatacg tccagtccta ttgtccatca atacatttcc agtctccact
    12181 tggctaccag caccatcatc taacatatag ccgtaatttt ctacgccggt ttgcaatgca
    12241 gcatagatat ctttatcgat cgtcgaatgg atcgtatatc ctttgttttg gatcgtttgc
    12301 tgtgcttttt gataataagc ttcactgacc gatgaatctg acatatcagc attatctttt
    12361 tctgccaatt gttgtgcaat aatccttgtt gcttcgttca tcacggtata atacaagaat
    12421 tctctgtcag tctgttccgc gatttgttgt gggagaaagt cttttgtaag atcataggct
    12481 ttcgcttctt cgtattgttc tttcgtaatt tgtccttcac ggtacatact gaataaaaca
    12541 aaatcttttc tggcaagacc ggcagaaaga tcttctttta gcgcgcctgt attcgtgtag
    12601 ggactatacg tgatagggct ttgaggcaaa ccagcgatat aagcagcttg tggcagagtc
    12661 acatcttttg catctacacc aaagattcct cttgcagctt cctggactcc agcaatattt
    12721 tgccccttat tattccgacc aaatggcgag acattcaaat acgtagcaat gatctcatct
    12781 tttgaaaagt atttttctac ttgtgcagaa agaacgatct cgtttgcttt acgtttgaat
    12841 gttgtttcat cggttaggat ctgctgtttg actaattgct gtgtcaaagt ggatcctcca
    12901 gacgagccga tgccagtagc ttctgaaact aatgctcgta aaacagcttt agggacataa
    12961 ccttggtgtt tatcgaagta ttcatcttct gtactgataa tcgcggtttt caataaaggc
    13021 gaaatcttat cagatgaaat agtcgtccgc atcagatctg tctggatttt tgatatctct
    13081 gtattatctg cataaacaat ctttgacgtt tccgtaatgt ttcctagttc tgtctgcagt
    13141 tcttttttag tcggcaattg tgtgtcttcg acaagatagg caaagtagcc agcacctatt
    13201 ccgataccta gagctgcgaa caacaaaaga agaacagtaa taaaaacagt caatgattga
    13261 aacactctaa aaataacctt aggaacaaac catttttctt tttttcttct attctgtttt
    13321 tttcgagtat ttttgcgtgg caaaagggtc acctcaacta atggttttat ttcgctaatt
    13381 atagcaaata ataagacaaa aaaacagaca aggctgtttt tgacaggaat ttctttttcg
    13441 aatgagataa aacgtgagga aatgatcttt tttatgaaag agttcttaag cttttcttta
    13501 gagaaagttt cagttactat ggagaacagg gcaaagaaaa acaaaccatt ttctttgtac
    13561 atcatcttac caatatatag gaattgttcg aaacaattgc ctttcaagga ggaatccatc
    13621 catgttatta ggctcacacg tcagcatgag cggaaagaaa atgctgttag gcgcggcaga
    13681 agaagcagcc agttacggat cttcgacctt tatgatctat acaggcgctc ctcaaaatac
    13741 tagaagaaaa gctatcgaag agatgaatat tgatgccgga aaagcattta tggcagaaaa
    13801 cggactaaca aatatcgtcg ttcatgcgcc ctatatcatt aatttgggaa atacgatcaa
    13861 accagaaatg tttccatttg ctgtacaatt tctgagagaa gagattgttc gtgcggaagc
    13921 tttaggtgct actcaaatca cccttcaccc aggcgctcat gtaggagcag gggcagaagg
    13981 tgggattgct agaattattg agggtttgaa tgaagttttg acaaaagaac agacggcaca
    14041 gatcgctttg gagacgatgg cagggaaagg tacggaaatc ggtcgaacat ttgaggaact
    14101 agcgcaaatc atcgatggag taaaattgaa tgaaaaatta tccgttactt ttgatacatg
    14161 tcatacgaat gatgccggat ataatataaa agaagatttc tcaggtgtga tggaagagtt
    14221 tgataaggtc atcggattag atcggcttaa agtactccat atcaacgatt ctaaaaatcc
    14281 acaaggttct cataaagatc ggcatgccaa tatcggtttt ggaacaattg gatttgacac
    14341 attgaacaaa atcgtccatg atccaatgtt tgacaacatt tcgaaaatct tagaaactcc
    14401 atatgtcggt aaagataaaa agaaagctta cgcaccatat ggaaaagaaa ttgccatgtt
    14461 caaagcgcaa gcattcgatc ccgaagtgtt tcctgagtta ttagcagaag aagtcacact
    14521 ttaagttttc tatctgacag caacaaaaaa ggattgggca gtctgccttg tccttttttg
    14581 tttaagaaac aaaggagtgc tttttgcagt cgttttcgcc atgctccttt cgccccctct
    14641 ttttaaattc agacaaacag ggtatactga tagaaaagta aaaaacgaaa agattcacaa
    14701 gcaagtagca aaacgtgatt gaaaggacga aaagatgctg aacactttaa aacccttgat
    14761 caaccaacta aaaaagaaac atacatactt tattcttggc atagccgtat tatttacaat
    14821 tattcggttg tatttatata gtaaagcaat ctatggaaca gatatgtcgg caacctatga
    14881 tgatcagctg ctgattcact atgctcaaaa tattttggat ggtaaatggc taggggagta
    14941 cacgactacg acactttcaa aagggatctc ctacagcttg tttttagtac ttgcacataa
    15001 actgcatctt tcttatgcag ttttgttcgg catgttcagt attcttgcca gcatattggt
    15061 tgcactagcg tttaaaccga tcgtgaagaa caagtatctt cttttaagta tctattttta
    15121 ttttttgttt tctccgatta gttttacaca tgagtattcc actcgaacgt atcgtaatgc
    15181 tctcgtgatc ccggctgttg agatcattat cgcttgttta ctagctattt attaccgtaa
    15241 atatagttcc cgcagaaaat tgctgccgtg gtttttaggg ttagctgttt cttttccttt
    15301 cttttggttc attcgcgaag attccatctg gcttcttcct tttactgtag tcgcgttagg
    15361 catcagtttc gttcagatct tgctaggaca agacttttct ttcaaagaag caaagaagtt
    15421 aaaagaacga ccatttctcc tttcgaaaca agaatggctg aaactttcat tgtttttact
    15481 ccctctagtc ggtttctcca tcgtctatca gtggatcgaa tataaaaacg agacaacata
    15541 tggtattgcc accatcaatg accgttcgag tggtgaattt ggacgtttga tgaaacagct
    15601 gatccgaata gatgacgaaa cgaacttaaa taaagaaaac agtgagatat gggtctcgca
    15661 tgctgcatta aataaagcat tagacgcatc tcctacattt catactgttt ctactcgtat
    15721 tgacgaattg tatacgactc atgcgtggac ccaaggcgga aaagtaaaag aattaccagg
    15781 agatatcatt ttttgggcat tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa
    15841 tgcaaaagaa accaatgatt tttggaaaaa agtcaatcaa gaattagaag aagcctatca
    15901 aaagggaact atcaaaaaga aaaaagaatt ctatctgatg ggatcagggg atggaaagac
    15961 gatgaatgat ctgccaatcg tatttgcttt tctgaaatca ggatatgcct atgcatggcg
    16021 atacaaagat ttccatcaag gagggaatta tagtttcggg acgcaagaac aagtcgaaca
    16081 ggcccaagaa ttattgaatc ttccactaat ggataattgg atcgatagca gtgatccgag
    16141 aacacctgat ttccagttga caaaaacagc aaaagtcgcg aatgtcatga taaaaattta
    16201 ccaagttctg gccattcccg tgctgattgc ttcaatagca gggatattac ttatgatact
    16261 aggaaccgta tttgcaaaag ctgatcgtgc ctattatagt tcctctttga ttgtagtcac
    16321 cggattaata ctgacacaat tggtttttct tttcggtgtc tcgtggtttt gttcttatgc
    16381 gccagaaaaa aaggattatt ttttaagtgt ctacactggc gcaggcgtgc cattaacaca
    16441 attcatcgta gtaacaagca tgctagtttt cagtaaattg aatctgaaaa aagctttatc
    16501 ttctaaata
//
LOCUS       DACEMO010000043        16423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEMO010000043 DACEMO010000000
VERSION     DACEMO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.044"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1073)
                     /locus_tag="IW728_001079"
     CDS             complement(<1..1073)
                     /locus_tag="IW728_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024453.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMI"
     gene            complement(1060..2907)
                     /locus_tag="IW728_001080"
     CDS             complement(1060..2907)
                     /locus_tag="IW728_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024454.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            2894..4531
                     /locus_tag="IW728_001081"
     CDS             2894..4531
                     /locus_tag="IW728_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1024455.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(4676..5416)
                     /gene="truA"
                     /locus_tag="IW728_001082"
     CDS             complement(4676..5416)
                     /gene="truA"
                     /locus_tag="IW728_001082"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1024456.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(5607..6242)
                     /locus_tag="IW728_001083"
     CDS             complement(5607..6242)
                     /locus_tag="IW728_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1024457.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(6389..7630)
                     /gene="sstT"
                     /locus_tag="IW728_001084"
     CDS             complement(6389..7630)
                     /gene="sstT"
                     /locus_tag="IW728_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ1024458.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            7828..8826
                     /locus_tag="IW728_001085"
     CDS             7828..8826
                     /locus_tag="IW728_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024459.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8839..10857
                     /locus_tag="IW728_001086"
     CDS             8839..10857
                     /locus_tag="IW728_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ1024460.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(10917..11633)
                     /locus_tag="IW728_001087"
     CDS             complement(10917..11633)
                     /locus_tag="IW728_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1024461.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(11860..12402)
                     /locus_tag="IW728_001088"
     CDS             complement(11860..12402)
                     /locus_tag="IW728_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1024462.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(12407..12634)
                     /locus_tag="IW728_001089"
     CDS             complement(12407..12634)
                     /locus_tag="IW728_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAQ1024463.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(12713..14593)
                     /locus_tag="IW728_001090"
     CDS             complement(12713..14593)
                     /locus_tag="IW728_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1024464.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(14782..15396)
                     /locus_tag="IW728_001091"
     CDS             complement(14782..15396)
                     /locus_tag="IW728_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1024465.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            15538..16098
                     /locus_tag="IW728_001092"
     CDS             15538..16098
                     /locus_tag="IW728_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1024466.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         5255 a         3103 c         2559 g         5506 t
ORIGIN      
        1 aatatcatcg ttaatatcag agaagagaat attatttgca agcttaagat ccgccaagga
       61 aattgtaaca atttggctgg cgtattttgc agaagatacc atggcaccaa actagttgta
      121 gctagccaag tcaaacacgt accataataa atcttttttt ctatgtttgt taatttttta
      181 aataaaatca ggggaataca taaagataac agaccaacta gtcccattga gtaagtagtt
      241 aagtcgttgt tcatagcttc agtcagacta tcgaacacat tagatgcatg agcatataaa
      301 tttgtcacgc taggtttgtt gattttttgg tagagcattt gttcaaacat cggataccaa
      361 aaagcactcg tcagaaccaa tgtcattcct gccgcactaa ataatttttt aaatatagca
      421 ataaaatctg cattcttgaa acctttagaa accaaacgaa taaaaataaa aatgataatt
      481 aatgctgtac tcattaatgt tgatagtaca tggctgtaca ctagcaaact catactcaat
      541 gctagcttta cagacggata cttttcttct tttccagaga tgatttgata agcataaaat
      601 aatacaggga taacaagtgt tattgcgata gcttcggcaa ttgcggaacg atagtagatg
      661 tctactgtcc gataagcaga gaaaatatag agacaactaa ataaaaatgc cgcttttttt
      721 tgttttaaaa accgctcgcc ataatgataa gctacgagtg ttgtacacaa gttcaataac
      781 caaacgtaaa taatatagct tacaatcaaa ttttttgtca tccaatataa cattacagct
      841 ggaaaaaaag tcagaaaggg ataaaaataa tttacaccat atccagtata attgaatgtc
      901 tcataattaa tcggactggt aaaaatagtc gataacccct tgattcgaga taagtgaaaa
      961 gaaagatctc cagtggtata aatactctgt gtatttttga aaaagatgaa agaatatata
     1021 ctactaacta taattaatcc actcaatatt actacttttt tatttatttt cattcttctt
     1081 gtttatctcc tttttagtca catatcgaga atctttcaac ttattaggtg caaatcgttt
     1141 aaaacttgaa tacaccacta aacaataagt cagaatactg atccataaag ccatgagagc
     1201 agttggagta tattttgttg taatttctat cttattttct ccagcattta catccacatt
     1261 cactaagcca tgttttttat aagagtcatt tacgacttta ccgtttactt ttacctgtgt
     1321 acctaaataa gcatatactg gtactgtcac tttttgcttt ttgttgcttg ccactgtgta
     1381 agtaatgtta ttatctgcag ctttatactt ccctttttcc cacgattctc ctacttttac
     1441 ttcatgtttt tctaagtgag gcctttcttt tatcgcttct gttggggcgt aatcgtataa
     1501 tcctcccata tctgatgttg tataacgtgg gagtgtttct tcattgataa caatatgacc
     1561 gtgttttgat tggataactt ttgcgtaatt ttcttttgct gctatagacg tgacgagtag
     1621 aaacaccaca gtagcgccaa tgattaacca tgaatatttt ttattcatag tctttttcat
     1681 aaaaacaagc gttatgatca ttgaagaaaa aaagatttgg atacctaaga tacgccaagg
     1741 aaactggagt aagcttatcg gtgttttttg aaacaaaaac catggaaata aattagttac
     1801 gcatacccat gtagaaacta cgccaacata gatgattttt tcttttttct ccatttcttt
     1861 cagtatgaat aaaggaaaaa tcaacgcaac caatcctaat aaccccatag tgtatgttgt
     1921 aatatcgtta tttaacgctt gaatgatact atctgaaata gaaagtgctt ctagctgcaa
     1981 atctgttttg aagggactgt gtatttcttg ataattcatc tgctgcaaca taggatacca
     2041 aaaatagcta gttaatgcac aagtcaaaaa aacagccttt actaaattca cgaccacaaa
     2101 ttttatatcg ttttttattt gcttgcttaa aaacaaacga actataatca tacaagcaat
     2161 caatgcacta cacaatagtg tagttaagac atgagtatac acaagtagac ttatactgac
     2221 agataaagcg atgacttttt tttctctatt gaatatcaat ccataagcat aatataaaac
     2281 agctgggata atcgtaagag cgattgcttc tgcaattgcg gatcgataat agatatcaac
     2341 tgttcgataa gttgcaaaag tatataaaca actaaataaa aaagcagcat ctatcttttt
     2401 aaaaaacttt tctccataat aaaaactaat cagaatcgta caaatattta ataaccaaat
     2461 atatagaaca taacttaata ttaagttatt tgaaattcca taaaaaacga ctgcaggaat
     2521 tattgtaaga aaaggataaa aatagttcag tccatctcca taattgttaa atgttgtata
     2581 gttgattggc ccttcaaaaa tagaagataa ccctttgatt cgtgaaagat gaaaagataa
     2641 atcacctgtt gtataaattt cttgagcatt tctcataaaa tttactgaat ataaagtact
     2701 aagtaagata agaactacta aaagaaatgc ttttttactt attttcatag atttgttgtt
     2761 ctcctttttt aattctattt tcagtatact agaattgtct ttttttaata agttttgcgt
     2821 taaatttttc atagcactag tatgatatac tagaaaagaa tcaattttta ttacttttaa
     2881 ataggagaaa aaaatgaaaa aaaacaaaaa ccaaataata gatatcattt ttatgttatt
     2941 tctaagtttt gcttacgtca tccctctatt catgtctaag ggaatctatc atagtgtcaa
     3001 tcaggatact tactttcatt tatctcgaat cataggacta gataatgtat ggagttctcc
     3061 agtaaacttc aataacttcg atcatcatgg aacaatgatg aatatttttt atccatggct
     3121 tactctttat ccagcttttt tattttataa aatgatggga aatcttgttt taagttataa
     3181 tatctactat ttttttatca cttttttaac aatggtcgtt tcttattttt ctatgaaaca
     3241 aataaaaaac aatcgttata tttcgctgtt attttcgatt atttatacat tttccgccta
     3301 tcgtgccatt gacatattcc atcgtgcttc attgggagaa gctgtcgctt taacattttt
     3361 gcctttgata ctaatggggt gttacgaaat ttatattaga gattaccaaa aatggtactg
     3421 gttgtctatt ggcatgacgc tagtagtgta tactcattta ctttctgtcg ctatggtttc
     3481 tgtatttatt ggaggaacgt tgtttctttc tttttatttt tgggaccaaa aaatcgctag
     3541 actcttaagt cttttaaaag ctaccgtcct cacctttttt ttaagtgcgg gattcttaat
     3601 tccttttatt caacaatcta gagcacaaga attgaaagtc ccactgggaa aagaattaag
     3661 tggtatggct cctagtgata tgctgacaca tattttaaat aataactata acaactatac
     3721 tattggatta ttcttatttc tcggattgat tggtgctttt atttttataa aaaaattaac
     3781 tgctgatgat ttatttattt tctttcttgg tgtatttgtt cttttttgtt caaccaattt
     3841 atttccttgg caacttttta accatacacc agttaagagt ctacaatttg tctggcggtt
     3901 aaatggtttt tcttctttat ttatagccta tacgatgagt atagtgatct attatatttt
     3961 ttctactaaa aataattcat ggaaaataat ggtctttacg tttcttgcac taatattgca
     4021 tctctcagga gtaagcaatt ctatccatgc taacaaagat tcattaacac ttatagcacc
     4081 tgaagacgct gttgctatag ctgaaaatta caatcatact gattatgcaa acaaagagtc
     4141 aatctatcat cctgatatga ttaataatga tcaatattta ctaggtaacc aggaaataaa
     4201 tccaacaact cattttatga gtaacaaact tacaattgaa ttagataatt cgaataataa
     4261 aaatactgtc ttgacgacac ctatttatcg ttataaaggg caagtggctt ctattaatgg
     4321 caaactagta caaacaaaat tatctaagtt tggcacaact gaattaacta ttcctccagg
     4381 tataaataaa gtcgttatta cttatcagta cacaaaacta gcaattgctt caagatattt
     4441 atctatcgtt actttgattt tatttttact ttatcgattt actttttcta aacaaaaaca
     4501 accgagaaga gaagtacaac attctcacta gatttttatt taagaaagac tttttagctg
     4561 tacacataaa caggctaaaa agtctttttt tatatttctt taatttgctt catacattat
     4621 ttaacatcga tatttatact gtgaaacatg cgtttcatcc attatcaaag tatttttatc
     4681 gataataaac ttcatccaaa aacagacctt gtgcagggac ggtttctcct gcatgttgcc
     4741 gcactttgct ttcaaatatt tcatcgattg tttctatcgg catggcacct gtgccgattt
     4801 ctagtaatgt ccccatgata atccgcacca ttttatataa gaatccgtct ccgacaaaag
     4861 taaaatgaag catttctccc tttcgttcga tagataattc atcaatcgta cgcactgtgg
     4921 atttttttga ttttttcaat gcagaaaaac caatgaaatc atgtgtaccc actaattttt
     4981 cgcaagctgc attcagcttg tccatatcca actcttgagg gaaataaaag ctgtggtttc
     5041 gctcaaatgc tgaaggaatc acgttgttcc aaacataata gctgtatttt ttgcccaccg
     5101 cattatatct tgaatggaag cgctcaggca tttcttcgat tcttttgatg acaatatccc
     5161 gcggaaggta acgattcata aagtcgatca tttctgccag tccaatttct gtgtttgttt
     5221 taaaatttgc tacttgtcct cgcgcatggg tgccagcatc tgttcttcct gatccaataa
     5281 tttctatctc ttcatttgtc atctgatgca atactttttc gatttttcct tgaatggttt
     5341 tatcgtcatc tccaagtctt tgccatcctg aatatctttt tccgtcatat tcgattgtca
     5401 acttgatgtt tctcatattt tagtggctcc tctgtcttcc atttggtctt ctctctacct
     5461 ttttaagatt gtagcacaaa atatagctcc ttgcttctct aagacagaag ctatacaaga
     5521 aaactgtgga aatatatatt tcagaaaaac aaaaaacagc caccggatga agtatccgct
     5581 gactgttcaa tttgacggct caagttttac tgtttgagat gcaagatact caaccaggca
     5641 atgggcacat aattaaagaa aaaactgatt ctttggcaaa aacctatttt attcagtaat
     5701 ggccaagaat ttatagaagg aattcttgcc agtccataaa tcgccgcaaa aagcaaactg
     5761 atcacagaca gtccaaggta taagtggctt acgtccattt gcccattttt tcgatagatc
     5821 aagaaaagaa gaactggaaa caataaaaaa ccactgtaac ctaaacctga tccgatatta
     5881 tggacccatg tcgagacatt ccacgaagat tcatgggtat tgatactgaa caaacctgta
     5941 aagatacaat cacctatacc atagaacgcg atggctgctg taactagggt agctaacaca
     6001 cgagatgtac taacaaatgc agcatacaat gcaggcaatg acaggatgaa aaagatccct
     6061 gaaaccacag accaaataag aaacgctttt cttacaggac tggaaacctc tccaaataca
     6121 ctgatcacgc tcttcatttg atctaattca ggataataca atcctaatac gtacggagtc
     6181 aaaaaatcac tcagcacgcc taataataaa aagaaaaaac caaatttacg taaaaaatcc
     6241 atgatacgcc tccaataaca attacctatg cttttaattg ttattataaa cgactttatc
     6301 ttgatttaac agaaacctct atttacacga acatctatat aactcattaa aaactcgaat
     6361 cattttaaaa atactaaata tctaacgttc aacgttcact taatccatct aaacaatctt
     6421 tcaatttgat tttttcccca cttcttttac gcgcgcctag ctctcctatt gcagtaaaca
     6481 acagatcact tgacgaattt aaggcagttt ccattgaatc ctggataaca ccgataataa
     6541 atccaatacc gacgatttgc atcgcgacat cattagagat accaaacaaa ctacatgcta
     6601 atggaatcaa taacagtgaa ccgcccgcaa cacctgaagc tccacaagca gctaaagcag
     6661 acaaaataca taaaaggact ttcaaaacaa aaggtgcttc aatccctaaa gaatgtacag
     6721 cagtcaacgt catcaaggaa atggtaatcg cggcaccgcc catattgatc gtagcaccta
     6781 acgggatcga tatggaataa gactcttttg tcaatttcaa tctttcagcc aacatcatgt
     6841 ttatcgggat atttgcggca gaacttcggg taaagaaagc gggaagtgcg ctttctttca
     6901 aacaaaagaa aatcaatgga tagggatttt ctttaataaa caaaaacgtg aggaatgggt
     6961 acacaactaa tgcgacaaac agcatcgtac cgatcaaaac aagtaaaagc tgcagatact
     7021 ctgccaatcc agcgattcca gtcgtagcaa ttgattgata aaccaatcct aaaatcccta
     7081 tcggagcaaa tgcaataatc acttgtacga cttgtgacaa cgctatagat atctgatcca
     7141 ccaactcttt tgtcgattct gaacgtgctc gaaaagctag tcccaacaat acagcccaga
     7201 ataagacgcc aaggtaattc gcatcaataa gagattgaac aggattttgt gtgacattcg
     7261 tcaacatagt agataaaaca ctttccaaac tttgcggtgc tttttcagat actgtttctt
     7321 gtaaagtaat aggaacagta aaaattgaac ttacaataac tgctacaagt gcagaaagca
     7381 atgttcccac taaatataag atcaaaatag gcttcacgaa tgtcttattc ccaattttat
     7441 gcttggcaat tgacgccatc gtcaagaaaa aaaccagaat aggtgcgatt gccttcaagc
     7501 aactgacaaa caacgttccc aatattgaaa taaatgtcca tgtggggaaa aagataccaa
     7561 acaatattcc gactaaaata ccaacaacaa tacgttgaac taacgacata tccaaaaact
     7621 tctctaacaa atgaccaccc tttcacaatt ttaacaatca ttgtatcatt ttatgcaaag
     7681 aaactaaagg tataattttc taaaaattct aacaatacaa acaaccattt cttgctaatt
     7741 ttgtactttt tctgtctaca cgttgttttt tgagatattc taagttagga tgttattaat
     7801 gggcaactta tcaggaaacg ttttcttatg tttttgtcca atttttttgt gaggtgttta
     7861 ttcttgaaaa aaataaagaa ggagagttat ggcttcctga caatctttgt cgcttgtata
     7921 atgatggttt tacagcgtat attgatttca gcagtcacta ttcctacata tgcaacaaac
     7981 cctggtcgat tctacatcta tacattttta caaacttccg ttgttattgg tgcaaatttg
     8041 atcccacttt acatgggtta ccaatatcaa aaaatcgccc gtttaaaagt tttacattat
     8101 ttaagtcgtt ttgcctttat attcctaata gccacactag tttgcaatat ctttttttac
     8161 gtacaagctg gcagcttgaa tattcgtgat tattggctta tcctaacacc tatcagtcag
     8221 aattacttta cttatgctgt ttcttgtact ttgctttttt gtagtattcc ttatacagtt
     8281 ggatttttaa ataaattatc gaaaacaaca attaaatatc tactattatt tttaacaatt
     8341 ggattagttg gctgctctac tctatttaat aaagacccgt ttgcattaca gaatggacag
     8401 agtattttat ggatttttta tttattttta atgggttatg gtttgaaaga atggaactgg
     8461 aaagaaaagg tccgctataa gcttcctcaa gtatttatca gcttgattgt tttattcggt
     8521 ttgattattt tgatgacaca aatttcactc atcatcagag gaaatacaga tacagcgctg
     8581 aggttttcta aaccatattc tctcttcgca atgtattata ctttctcttc gtttttattt
     8641 ttagaaatgc tatctgataa attaggtata aaaggaagag gaaattttct tgctgtattt
     8701 ttgattgtca ctcaagttat atgtaatttc cctttagttt cttatcgcgt atctacattt
     8761 atgaaacgtg aatttccaaa ctcagggaag gcatggctga taaatatcgg gcagtttttt
     8821 atttgatatt tattagcagt gtccctccta gttatctttt tattttatat acaaaaaatc
     8881 cgtttcattc atcgtttcat cgaatacttc gcttttgatt cgattgaaca attaactcag
     8941 cgttggaaaa aaagaattca ttggcttttt gttcataaaa gttatttttt agttgcagca
     9001 tttttttact gctttacctt cgtgcagatt ttcgttttag aaccaaaaga aggttggaaa
     9061 gaaactatcc aggttgcctt aaaaatattt actcagcgac aagcacccat gatcctgaca
     9121 atcatcatta ttatgggatt tttcttctta ctattgttga tttccaatcg tttttggtac
     9181 gccttgactg ctaccttgat cattaactta ctgctcacta tttcgacagt aatcaaaatg
     9241 gaaatgcggg aagagcctgt ctttccttca gatttaaaaa tgctgaccgg attgtcagaa
     9301 ctattatcga tggttagccc agtcttatta atcgtgggcg gattgatcct tctcctatta
     9361 ttgattacta gtattattgt acaacgtaga ctacaaaaac agtattcctt gaagattcat
     9421 tggaaacgtc gatttatcag tatcgctgta ttattaggat gtttttcggg tgtctttttc
     9481 attaatcaca aaaattcgcc ttctttcttg ctgtttaatt tatttaaagt aaacaagact
     9541 ttctttaacc agcaaggcgc cgtaaaagat aacggaccag tcattcaatt cctaaacaat
     9601 atcgacatta aaatcatgga agaacctgct ggctattcga aagaaaaaat taatggaata
     9661 atgaaaaagt acaacacaaa ggcagatgaa atcaaccaaa caagaagtga ctggttaaac
     9721 aatcagacga tcgttctcaa tttaagtgaa agcttcagtg atcctgcacg tgtccctaat
     9781 ctgacagtac ctacagaccc tattccttac atcaaaagca ttataacaga aggaacagga
     9841 ggattgatgt tatccgtcgg ttatggtggc ggaacagcta acatggaatg ggaaggactg
     9901 acaggcttga gtatcagtaa cctgtctgct tcgttagtta caccttatac ccagctagtg
     9961 gagcgtcaga aaataagtcc aaatattacc gatctattcg atgaaaaaat agccatccat
    10021 ccttttacag ctgctcttta tcgcagaaaa caagtgtttg ataaatttgg ttttgacagg
    10081 ttctattatt tagacagtcc ggataaatta acttatacgg ataaaataga acaaagccca
    10141 cgcatatcgg atgattcagc atacaaagaa accttgaaag ctttacaaag caatacggaa
    10201 acgtcacaat tcatccaatt atccactatg caaaaccata tgccttatga tgattactat
    10261 agtgaattga actacactgc tgaaggaact gctgtcattc ctaatagaag aaaagaatta
    10321 caaacttata tgcaaggcct tcactatacc gatacagcag taaaaaaatt catccaagaa
    10381 atcgacaaaa tcgataaacc gattaccttt gttttttatg gtgaccattt acccgcaatc
    10441 tatagtggga atgattcgaa aaaatatgga ttagaacagc accagacaga ttatttcatt
    10501 tataacaaca agtatagtcg tgaacaagca gctacgttaa ataaagaagt tgttgctcct
    10561 aataactttg gtgctatggc tcttgagcat gcgaatatca aagtaactcc ttactatgcg
    10621 ttgttgacag aagtatctaa tcatctacct gcattaacaa ttgatcctac agaaagcttg
    10681 tctaatcgtt ttaatggaaa acaggtcttt gttacagagg atagtcaaat tatccaagaa
    10741 aaaaatttaa ctgagaaaca aaaagaaatt ctagaagatt atcgtctgat acaatatgat
    10801 cttgttgcag gtaaccagta cagtgcaaca tgggcgacac aaaaaataaa agactaacaa
    10861 ataaactgag caaggggttg tgataaaaga catgtcacaa ccccttgttt atctcattaa
    10921 tttgcttgtc cataaacgat ttgattaaag ccatttttca attcattatt cgctgtctcg
    10981 ttgaagtaaa gataattatc gggattcccc tgattttgtt gtgtcaccaa tacttttggc
    11041 tgattttgct gaaacccgaa tagatagacg tgctttttca gatatggttc tgtctctgca
    11101 tcggaataga cggctgtgat ttgatagtct gcttgacctg tcccactttc tgaccattct
    11161 gcagaaacag gcactccacc aatagccatc ttccactccc cgttcaaaat agcttgaggg
    11221 actttaagac catataagtc cacactcatt tgattcgtat aacttttgta ttgttgtccc
    11281 attgtttggc cccatttggt cataaatgcc tctaattgtg cggatttgtc tgcattccac
    11341 agtgtttgtt caacttgttc gctgctttct gaagaggaag atgaagcctc tacagaagaa
    11401 cttgaagtag atgtttgagt tgtttcagtc gttttggaag actctgacga agtagaggta
    11461 tctgctgttt ctgtacttgc tgcagttgac ttgctctctg tgctagttgt tgtagtagtt
    11521 gcaactgttg ctttcgctgt ttcttttgtt gattcttttt tcgattcatt cccgttggta
    11581 caaccagcta acaaaaaagc tagaccaatt gttactaaaa tccttttttt caattaaaaa
    11641 actcctttcg attactccca cttctttatt ctatcaaata agcaatgtga ttttattgat
    11701 ttttcgtatt ttctcatcaa tctttcaaat cttctaaaaa ctgagaagca cccgctgatg
    11761 ttgtctgtaa tacatcagcg ggtgcttctt ttattttaat cttcgtgatt ttccaattta
    11821 cctcacgctt tatatttgta tttgaagaaa catagacttt tagtcatcat cgtcttcctc
    11881 atccaaatct tgtcggacag tttttccttg gagtctttgt acttggtaga tgatttcttt
    11941 attgaaactt aaaagttcaa caagcaattg ttgcaagcca tatttttctt ctttttcagc
    12001 taatttgatt gcccgtgtga taaagacatt ttggaaatca aaatcttttg caaagttttc
    12061 caacattgtt gaggctggta aatatttgtt ttgaccatgt tcgtaaattt ttccataacg
    12121 catgacttct tctaaagtag acggaacgat ttcattttca atcactaact catgagagat
    12181 tttattgaac catctcgctt cttgatcagc atattctgaa aaaagggtac ttaattcttg
    12241 cgaagatgta cccaaaacat acaattgtgc ttggatcaat ttcatttctt ctaatttaaa
    12301 atttgctaat acatgcccca ccatggcgcc agcagttggt ttatgatggt cgatatctgc
    12361 ttgttttgtt tcttgaacaa atttttcttc cactatattc attgatttac gcctctttta
    12421 cttttgctga ttggacttca taacctaatt tttccacaac tgcagctaat tgttcagggt
    12481 ggacttcatc tggatcaaag tttgctttga ttttgctcgc attaaacaaa actttcgtat
    12541 tgcttacccc aggctgttga gaaaccgcat gttcgatttt ttgcatacag cttggacaag
    12601 ctagtgtatc taattgcata ataatttttt tcatcgtagt tcttccttcc tttttcttga
    12661 tagtcttatt ctagattaca gcttccataa aaaaattgat tcttatcaag ttttacgatt
    12721 tttcttcttt gctttcgtgt ccaaaataca ataaacgcat cgcgttaaag ataacgatca
    12781 agatactagc ttcgtgaacg aacatcccac tagccatgta aataaacccg gcaaataagc
    12841 cgttgagtaa aaataagacg acaccaatgg caatcactac attttcacgg gtatttaaaa
    12901 cagttttctt agccaagcgg taggcatgaa ccaacgattc aaaattagat tgcatcaaga
    12961 caacgtcact cgtttcgata gcaacatccg ttcctgatcc catagcaatc ccgatatcag
    13021 ccgcagccaa agagggagaa tcattgattc catctccaat aaaagcaacg cgcaatcctt
    13081 gttcttggta ttttttcaca aattgaacct tttcgtctgg taataattca gcatggactt
    13141 catcaatgcc taacatatca gctacataat cagcagtcat ctgattatca cctgtcagca
    13201 tcactaactg ttttgctcct gcttgtttca acttagctaa ttgttcagcg acttctggtc
    13261 gaatcacatc tgacacacct atgatttgag tgattttttt gtcgattgct acaataatga
    13321 ccgagctgcc tgtttgttgt aattgatgta aatcttgtac ttgctttggt gtgagagaaa
    13381 gtttgtgagc ggcaatcaat ttggtgttgc ccgcacaaac tgtatgacca tcaatctcag
    13441 ctgtgattcc ttgtcccttg attgtctgat tatctttgac tgctaattgt tgaaaatcaa
    13501 tgtctttctc ctgagcataa tggatcacgg cgcgccccag aggatgatcg gataacgttt
    13561 ctaatcgagc cactaaagct aagctgtccc cttcatcctc tgtatagttc ttgatagtgg
    13621 atacagcagt agttcctttg gtcaatgtac cagttttatc aaagacaaaa gtatcgattt
    13681 tggaaaaagt atccattgct tcaccgcctt tgactaagat gccccgtttc gctccattac
    13741 cgattcctgc tacattcgaa acaggcgcac caatgactaa agcgccagga caaccaagaa
    13801 ccaaaacagt gattgctaaa cgaaaatcac gactaatcag accaacaacc aacgcaataa
    13861 gtaaaacggt tggagtataa tactgagaaa atcgatcaat gaatttttcc gcatgggatt
    13921 tggtatcttg tgcttcttct actaattcga taattttggc aaaggtcgta tcatctccga
    13981 ccttagttgc ttcaatcgtc acgtagccat ctgctaaaat cgttccagag aagacttcat
    14041 ctccactgat tttgacgact tgtcgtgatt caccagtcac tgcggcttca tccgcatacc
    14101 ctttcccgtc aatgactcgt ccgtccacag gaatagctcc accagttttt actaagacga
    14161 tatcgccttc ttccacgtca tcgatttcca cttcttcagt cgttccatct tcttggacaa
    14221 cactagcagt agatggggcc atctcggtca aagccttgat tgactcccgc gttttggtca
    14281 atgttttttg ttccagatag ctaccaaaca aaaacaagaa tgtaacgata gctgactcct
    14341 tgtattctcc gatcataaag gcaccaagca cagcaatagt aacgagtaaa tcaattgaga
    14401 taactttgac tttcactgcc tgatacgcat ggatcataat aggtatgacc gcaataatag
    14461 aagctatggc tagagcaatg ttatagaaca agacaaaacc agtaaatttt ccgataaaac
    14521 caaaaataat caacacagct gataaggcgg tgatatgatt tcgatgctct tggatccact
    14581 tttgtaattt catctttctc cctcttttcc tttcattctt acgagactaa gtatagatac
    14641 ttatccatca taaaaaattg accactatca agtttagtca aaaaggcggt tttcttttgg
    14701 tagaatacac tacaaaaaat agttagcaag aagactcact aaaagtgatc ttgccaacta
    14761 ttgtcgtttc tatcgaaatt ttcattgaat gattttcctg tacagcatac atcggagatc
    14821 tatttcttta tctaagattt ttatcttgct aattttttcc tttagtcttt caaatccacg
    14881 tcgttcatag aattgatacg tacagccact atctgtaaaa aggaatattt cttttccttt
    14941 ctctcttttt tcaaattctg ttagcaactt ggtaccgatg ccttttgttt tcaaatcagg
    15001 atttgcagct aaaaaaatga tctctccatc tggcgaatgc ttctttaaat atgctgcaag
    15061 catttgttca tttgctgtct cgtaagctcc tttactttct ttagcaaata tattttgtaa
    15121 aaaatcgaat atcttaacat atatttgctg tccaaaagaa cgatgtttct ttttttctcc
    15181 ttttatctcc gccagcaaaa cacctgccaa ctcgtcctct gcatacaaag caagaatttg
    15241 agttgctcga ttcaactcca tatacaaaaa atatcttccg taaagattca gcagaagttc
    15301 attttccata tatctctcaa agttcattcc ttcaacagca aaatagatag cttttttgta
    15361 atctttcttt tctaagggtt taaaagttag tttcatcatt tcgcctcctc aacttcaatg
    15421 attgtgttat acttacatta taatagacac agtgtctact atctataatc aatcatctat
    15481 aagtttgtcg attaagcgac agctttgctt tgatatgtcg gataaggaga taggtaaatg
    15541 aaaaagacag atagacgcgt caaaaaaaca gaaaaagctt tggctgaaac actatccaca
    15601 ttattagtaa ataaaaagat tcaagcaatc actatacgcg aactaacaga aacggcagat
    15661 gtacatcgtt cgacttttta tacacattat aaggatatct atgatctata tgatcaatta
    15721 gaaagcaact tttttcggga tctaaatgaa attctctctt atgatccagc ccatagctat
    15781 gaggaactat acactcgctt gattgactat gtttacgcaa atactttttt atatcgctta
    15841 tttatcctca accatggtga tatttcgttt cagaagaaag ttaaacaagt tattgaagaa
    15901 aactatctga acatatggct atatgaagac aagaaaacga ttatcacaga agaaatgaga
    15961 ttctttacta cgtaccacgt ccaaggttgt ctatccgttc tcaatcggtg ggtagaaagc
    16021 aattacaagt actccaaaaa agaattatta gaactcttac agaaactgaa tgatcacata
    16081 gagaaaatca tgccttgaat aaaaacatac taagcttagt taagaagaac cgtaaccttc
    16141 tcgtataaca actgaatctt atttagccaa attgtttact gaagattttt tgagtaaaat
    16201 ttctataatt cctacttatt gaaaagtagt ttgtagtgct gcttctccct ctctaagtga
    16261 tatatcttgt tgtaccaatc aaattcacct catcattaat aaattacttt taggctaatt
    16321 tattacaact tataggttga agctgtaacc aatttatagt catattatat ttttatcatc
    16381 cagtttattg aattcttctc gtttacctca attcattttc aag
//
LOCUS       DACEMO010000044        16415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEMO010000044 DACEMO010000000
VERSION     DACEMO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.045"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            59..403
                     /locus_tag="IW728_001093"
     CDS             59..403
                     /locus_tag="IW728_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1024467.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            408..689
                     /locus_tag="IW728_001094"
     CDS             408..689
                     /locus_tag="IW728_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024468.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            792..1106
                     /locus_tag="IW728_001095"
     CDS             792..1106
                     /locus_tag="IW728_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ1024469.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1084..2778
                     /locus_tag="IW728_001096"
     CDS             1084..2778
                     /locus_tag="IW728_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1024470.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            2798..3976
                     /locus_tag="IW728_001097"
     CDS             2798..3976
                     /locus_tag="IW728_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1024471.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            3939..4625
                     /locus_tag="IW728_001098"
     CDS             3939..4625
                     /locus_tag="IW728_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1024472.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            4625..5785
                     /locus_tag="IW728_001099"
     CDS             4625..5785
                     /locus_tag="IW728_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1024473.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            5795..6670
                     /locus_tag="IW728_001100"
     CDS             5795..6670
                     /locus_tag="IW728_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024474.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            6667..6978
                     /locus_tag="IW728_001101"
     CDS             6667..6978
                     /locus_tag="IW728_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024475.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            6968..7321
                     /locus_tag="IW728_001102"
     CDS             6968..7321
                     /locus_tag="IW728_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1024476.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            7311..7712
                     /locus_tag="IW728_001103"
     CDS             7311..7712
                     /locus_tag="IW728_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024477.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            7705..8109
                     /locus_tag="IW728_001104"
     CDS             7705..8109
                     /locus_tag="IW728_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024478.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8121..8726
                     /locus_tag="IW728_001105"
     CDS             8121..8726
                     /locus_tag="IW728_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1024479.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            8746..9108
                     /locus_tag="IW728_001106"
     CDS             8746..9108
                     /locus_tag="IW728_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024480.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9111..9293
                     /locus_tag="IW728_001107"
     CDS             9111..9293
                     /locus_tag="IW728_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024481.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            9310..12729
                     /locus_tag="IW728_001108"
     CDS             9310..12729
                     /locus_tag="IW728_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1024482.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            12780..13517
                     /locus_tag="IW728_001109"
     CDS             12780..13517
                     /locus_tag="IW728_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1024483.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            13527..15818
                     /locus_tag="IW728_001110"
     CDS             13527..15818
                     /locus_tag="IW728_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024484.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            15842..>16415
                     /locus_tag="IW728_001111"
     CDS             15842..>16415
                     /locus_tag="IW728_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024485.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMY"
BASE COUNT         5813 a         2396 c         3458 g         4748 t
ORIGIN      
        1 ccattgtatg gcaaaagaat agctaagtat taaataagat tgcgaaagga gatgggacat
       61 gaccgaggaa ttctatagat ggttattaca gttgataaga gaagatcgtt tggttaagtt
      121 ctatcagtct cctaaatggc gcaggcttag agagaaagcg atgaaacgag atcactatga
      181 atgccaagag tgtagaagac taggtaagta tcatagagta gagaacgttc atcatataaa
      241 ggaagtcaag gatagacctg acttagcttt agatttagat aatcttattt gtttatgtgt
      301 tgaacatcat aatgaagttc atggcagata tcttacagcg ttagataaac aagagaagaa
      361 gatagaaagc ttcgctaact tcgatgcaag tgaaaggtgg taagtgcatg atcatcaatg
      421 acaatggcag agagtatgat acagaaaaga ttgaagagta ttcatcttat actcagggat
      481 taattaaacg tttgatatac gttcgctatg taggtattag ggatctgtta tcagataact
      541 gttgtagtaa atacaaagtg aatcaagtaa gagaagcgtt gaataaagat aataacgtcg
      601 aaagaataaa aaatgttttt ggatatagta ttgaagagat taattattac attgacttcg
      661 ctgaagcttt cattccgatg gtgagataac ccccccttaa aataaatcgc aaattttttg
      721 ggggtgatga aacggagggg gctgtcagga aaagagattt tttcgaactt tatcatgaaa
      781 ggagggctaa aatgtttaaa aacgaattgt ctcaaaatcg ctacagagaa aaattacgcc
      841 gctctttaat aagccaattg gaaagtcaga aaacaaatat tgagccattc ttagataatg
      901 ttgatcgtta tatcagttta tgggaaacgg cgatatcact ggaagaagat atatccgaga
      961 acggcattag actggagaat ggtaaaaaga atgaatcagt agcgttgctt gtttctgtca
     1021 acaaacaaat gggattgatg ttggataaac ttgccattac tcctgaattg gtaggtgaag
     1081 caaatgaatc aattcctgag ttataagcat attgaaaatt ggttcaaagc tatagaagaa
     1141 ggcactatca aggtatgcaa agagcaatta ttgctaaaaa attatctaga agaaagagtc
     1201 tttactagag aagatattta cttcgataag cagatggtag aggattcaat caatatacca
     1261 gcacaatact ttccattcga attaattccg tgggaaaaat ttctacaatg ttttatttat
     1321 ggtgttcgat ggaaaaaaga taaaacacta gtgttcaata gatatctttc attaatggga
     1381 cgtggtaatg gtaaaactgg ttttgcttct tggaacaact tctttctact aaccgctaaa
     1441 cacggtatta aaaattatga tattgatatc tatgccaata atgaaagcca agcaaagact
     1501 agttttgatg atgtatttaa agtaattaaa gatcatcctg atttagataa aaaagtattt
     1561 aaagctacga aggaagttat tcaaaatatc gctacaaaca gcaaacttcg ttataacacg
     1621 gcaaatgcta gaacaaaaga tgggaagcga ccaggtgcaa accgctttga tgaaattcac
     1681 gaaaatgaag attattcaat gataaatgtg gctacttctg gtggtggtaa aattcgagat
     1741 tatagagaat tttatgatac aactaatggt catgttcgtg gtggtccgct tgatgacatt
     1801 atagaagaat caaaaatgat tctttctgga gaacttggaa ttgacaagga tggagcagaa
     1861 ttttctagtt tgtttccatt tatttgtcgc ttggataacg ataatgaagt tgatgatccc
     1921 gacatgtggg aaaaagcttg tccaactatt aattacaatg cagatttaaa acggaaaatg
     1981 tttcaagaat actctcaaat gcaacgtaat gctggtttaa gacttacgtt catgaccaaa
     2041 cgaatgaaca gacctatgga agatacacga tttgctgttg cttcatatga tgatgttctg
     2101 catacgaaag aaaaagaatt tcctgaaaaa atggatgaag tgataggaac agtcgatttt
     2161 gctgatagac gagattttgc cagcgttggg ttgctaggaa aatacgataa agatgtttat
     2221 tttacacaac atacttttat ccacgaatca gcccttcgat tacaaaacat caaacgagag
     2281 gttatagata tttctataga tcaaggaaaa tcacagatcg ttcatggaaa aaatatagaa
     2341 gctgattata ttgtaggttg gtttcttgaa atgagtaata aatattatat taaaaaaatc
     2401 gctatggata tgtaccgtgc aaaaatattg aagcccgctt tagaagaagc aggttttact
     2461 gtggaaattg ttcgaagcgg atctgttaca catggtatgt taaaagatct ggttgatgac
     2521 ctttttatta atcaacgttt attttttggt gacgatgcga ttatgcgttg gtattgcatg
     2581 aatgtatatg aagagcatat ttctaatgga aatatacgct atgaaaaaat agaacctgaa
     2641 actagaaaaa cggatggctt tttttcattc cttcatggtt tgaatttttt agatgatatt
     2701 tatgattctg ctcctgtaac agtcacaaat agctcagtag aaaatacagg aactggattt
     2761 actcctctag tattctaact tgaaaggagg tgagaaagtg gggatttttc aaaaggcggt
     2821 aggatacttc acaaaaaaag caacggttcc tttagaagaa tacttttgta aattgcaagt
     2881 tgattttgtg tatcgaaaat ttgcgattga aacttgtatt gatttgattg caaatgcgat
     2941 gagtaaagcg gaattcaagt catatgaaga tggaaaaaat aaaaagaatg atctttacta
     3001 taggctgaat gtagctccta ataagaaaaa taatgcaaca gaatttagaa aaaaactgat
     3061 caggagatta atattctaca atgaagtatt gatcgtttct ccgtctaata attctagcga
     3121 aatatttatt gcggatagtt gggatgtcac agaatatgca ttgaaagatg atgtgttttc
     3181 tcaagtgcaa attaacaaca tagtccttga tagagaattt ctagaaagtg atgttatcta
     3241 tataaaatac gcagatcaac aaattaggca actagtcgat gcgtattatc aagcgtatgg
     3301 gaaactcatt tctagtgcca tgaatgttta caagcgctct aacgctcgta gatacgtact
     3361 gaaagggaat ttattccgac cgcaagacaa tacaacacaa gatcaaatca ataaaatgat
     3421 gacatcacaa tttaaggctt ttatggaagc tgataatgca ggtgcggtat ttcaattaca
     3481 aaatgagtac acattagaag atttcagcgg aaacttccaa agcaattcaa gagatataaa
     3541 aaacttaata gacgacatct ttgagatgac agcagcagcg tttcacgttc cgaaaaacct
     3601 actaaaggga gacatgagtg ggttatcgga tcaagtggac gcttttttaa tgttcgaaat
     3661 cataccgatt gctgaactta ttcaggatgc gtttaacgct agtctctatg aagtagaaga
     3721 atacttgtca gggaattttg tacgtgtgga tacaactatg atcaagatta ctagcttcaa
     3781 agatttggtt gacgctattg atgtaggcat tagaaatgga gtatttacaa tcaacgaagg
     3841 aagagagcgc gttggaaatg atcgctctga taaggcgatg gcagatgaaa tatttataac
     3901 taaaaacaat caacaagtat cgaaaggagg tgaggcgaat gacgacaatg aaaacatttc
     3961 tagcagtaaa gaatgaaggc gcagtaccgc aaatttttat tcagggattt attggttcta
     4021 gttggttctt tgaagggaat actgacaagg gaatcaaaaa tattttggat agtctaggtg
     4081 atcaagaaga aattgaagta gtaattaatt caaacggtgg agacgtattt caagggattg
     4141 ctattgggaa cttacttaag tcaaataaag caaaagttaa cgttgtgatt aacggcttag
     4201 ccgctagtgc tgcttcaatt atcgcaatgg ctggcgatac tataaaaatc tacaacaatg
     4261 cacaattgat gattcaccgc gcttccacat acggagaagg aaatgtcgat gacttccgta
     4321 cgattgctga ccaactggaa tcaattgata aatcggtaaa ggcttcatat aaaacacgat
     4381 tcaatggcac agatgaagca ttgcaagaac ttcttgaaaa agaatcgttt atggatgcag
     4441 aaacagcttt gagttatgga ttggtcgatg aaattatcga tgcagaaaat agctcaggta
     4501 ctgaagctaa aaaagaacaa agcgttgaag aaattttgaa tgacgttaaa gaaaaaagag
     4561 cagaaaaaat tgctgcattt acagcagcat taaataaaac atttggacaa ggagatgtaa
     4621 aataatgaca gttaaaaatt taaaaggtgt aacagctgca agcgaccaat tgatgaaagc
     4681 ttttaaagat ggtaacgaag aatcttttag cgcagctatg gtaagcttat ctaaggaaat
     4741 tcaggataaa attttagaag aagcaacagc aaaaaatcaa gatcaattag tattaatgaa
     4801 ccgtggtcag cgtgtattaa ctacacaaga aacaaaattc tataacgaag tagtgaaaaa
     4861 cgaaggtttt gcaggggtcg aagaattagt gccagctact gtatttgaac gtgtatttga
     4921 agatttagaa caatctcatc cactattgca aaaaattact tttgttaaca caactggtgt
     4981 aacagaatgg attgtgtcac gtggagtcaa tccagcatgg tggggtaaac tttgcgaagc
     5041 tgttaaaaaa gttttagata atggctttga tgtaattaac atgaagcagt tcaagctatc
     5101 aggttatatt cctgtatgta aggcaatgct tgatttaggt ccagtatggt tagatcgtta
     5161 tgtccgtact gttttagtag aatcattgag aattgcatta gaacaagcaa ttgttgatgg
     5221 tactggtaaa gatatgccag tcggaatgat gcgtgacatg agcaaacaaa ctagcggaga
     5281 atatgctgaa aaaaaagcag aacctattac agctttagat gctgtaacta tgggcggttt
     5341 gatggcgcga ctatcaaaat tcaatatcga aggtgtgaat gatccgattt atcgtaatgt
     5401 gaatccttct gatgtggtcc taattgtgaa tccaactgat tactggtcta aagtattccc
     5461 agctaagact gtactaactg ctaatggaga atacgtacaa gtattgccag taccagtttc
     5521 agatttgcag tcaacggctg tgccagaagg aaaagcagtt attggggtag cctcagatta
     5581 cttcatgggt gtaggatcta cactaaaaat tgaagcttca gatgaatacc attttgttga
     5641 agacgaacgc atttatctag ctaaacaata tgcaaacggt caacctaaac gtaacgatag
     5701 tttcattgta ttagatatta gcgctttggg aactactact acaactacaa aaccaacaac
     5761 cacaacaact acaacacaag cgtaggtgat cagaatgaag tatattcttt gtcagccggc
     5821 aatcaatcgg tttaaatggg agcttgaagt ttgtttaact aatctgaaga aactaggaat
     5881 caaagatatc gtattgcttt tcagcagaca cgatgatcag attcctattt tttttgagaa
     5941 ggaatatggt gttgaagttc atgtgtacga tgatctgcgg gacgacaaag agtatattcc
     6001 ttcgattaaa ccatatttat ggtggaaata tttagaagaa gatcattcgc gtgaggacga
     6061 ccgatatttc tatatcgatt cggatgtcat tttcaataaa agaattaatt tgcgcaaatt
     6121 gccttctaaa gatgatgttt ggtattgtag cgactgctgt agttatctaa gtcttgatta
     6181 tattagaagc tgtgaaaacg gagaaaatat tctaaaagat atggcaaaca ttgtaaatgt
     6241 tacagtagaa tctttggaaa ctataaacac taattcagga ggcgcacagt gggttattaa
     6301 ccgtcctaaa gcgaattatt ggaaaaaggt ttatctggat tctaatcggc tatatcgcta
     6361 ccttagaggg caaaaaacaa atatacaaat ctggacagcc gagatgtggg cacggctttg
     6421 gaacatgatg tatttcaata ttggtcctaa agttcacgag gaattagact tttgttttgc
     6481 tactgatcca atagaaaaag ttaaagaagt aaaaatctta cacaatgctg gagtaacaac
     6541 aaacgatgaa gatttatttt tcaaagggag atacgtgact tctacgcctt ttgatgaaga
     6601 tttatcattt gtaaacaaga aaaaatgctc ttacgcatat gttaaagcaa ttaaggcggt
     6661 ggttagatga cgcctgaaca agtgactgaa gaattgctaa tagctgtgaa ggataatatt
     6721 tacgttacct ggaacgaaga agatgagtca attaaaaaga tgatagctaa aaatgctgtt
     6781 tatcttcaaa gtaaagtgag tacaacactt tctttttctc ctgaaagctt agaatacgga
     6841 ttgctaatcg aaagatgtag atacgactgg aatcgtgctt tagatgagtt tgaacaaaat
     6901 ttcgctagtg agttattagg tttcattcaa cattatgcgc tacaagaata tattgcaggt
     6961 gatgtgaatg gcgaataatc gtagactcga agaaacattc aacgatggtt ggttaaagat
     7021 tttgacgcaa actaccaaaa gaaatgaact aggaaaaaag attggtgtag aagatacaga
     7081 aatcacttct ttaaaattta gaaatctttc catgagagat agtgatataa cagctatgga
     7141 tgcgatggga tcgaaattaa ctaagaaagt aaagactcca tttcatccaa tcgccaagaa
     7201 atttaataaa gatcaatatt ttatcgtaat cgatagtatg cgttacaacg ttatctatgc
     7261 cgattacgat aatttttata tctattttta tcttgaaagt gtgggtgaat atggtgattg
     7321 ataattctaa agaaaaagaa cgtttaaata agcaaatttc tgctatcaaa acttccttag
     7381 aagagcattt tggcctcaaa ctctttcaag actccgtagg cgaggatgag ctacctgatg
     7441 attttaatta cttcattctc gaaacaggag aaatagaaat gatcactgag ccaaaatata
     7501 gcgtgggtca aaatctatat ctaactttct attcagaaaa tagagaagat ttaacaggag
     7561 attcactaga tattatttca ttgattcaaa atcgttcgat tcgttttcag agaatggatc
     7621 ccaatcattt aaaactagag aaccaagatc gctatatcga tcaattggta tttacgttta
     7681 gacgattatt gaagagtgat tgtcatggct aaaaatagtt gggagctaaa aataaatgga
     7741 catgatgaac ttcttgtgcg gatggaacgc tattcaagcg agagcgaacg actgattaac
     7801 gaagcattga aatcaaaagg ttcggctatt gcagtggata ggattacaga aaaaattcct
     7861 gtttctgaag cagatttaag aagagggcac caacacgcaa aaaatagtcg tccacttaag
     7921 actcaataca ttaatttggg tttcatcatt agacctacaa gaaaatttga gtatttaaaa
     7981 tatcctgatt tggggatagg tacttctaaa agaaatcagc cggacgaatt tatgagaaga
     8041 ggattaggtc ttgcacttga tccaattaca gaacttctga ttcgtcaatt cgataaatta
     8101 aataaatagg aggaacaaca atggctaaaa caacaacagt aacaactttt gacaatataa
     8161 gtattaaaag aatttctttt aattttaaga atgcaacaaa tgcaatttca actgattgta
     8221 atggacaatt agatggcgaa acagaaatgc agagaattgt aaaaaaatgc ggttcaacag
     8281 aagtaaaatc gaaatctaaa ccaatcaata tgacggtaac aattactgca catgtaccga
     8341 tggaagttta tcgacgcttc aatgggttga aacaagatga acgtattaaa tcgggaattt
     8401 actcttatgg tcctgattcc gtaggggaag atttctctct tgctgcagag atcgtggatg
     8461 atttcgaaga aaaaaataag ctgattggta tgttagcatg cacttcgaat acaggattaa
     8521 cattctctat tgaaaatggt gcggatgaag tagctgcgtt agaactagaa acaaaagtta
     8581 tgcaagatga atttggtaaa ttttatcatg aagcaattgt tgcagaactt gaagaagact
     8641 taacagatca atggatgacg aatctatctg ctgatgtgat taaaaaaact tcagttgtga
     8701 caactacggc cactcaatca cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac
     8761 tcaaaaattg aactaaacga tggaacaatt ttgaatttag aacctaaact gaatatcaag
     8821 aaattattga tgatcaatag agattttaac acagacgagt ttgcaaaaat gactgtggga
     8881 aaaggatcca tggatatttc tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc
     8941 caagcgaaca tgactgatta tatttcattc gatgaattta tcgataaatg ggattttgat
     9001 atggctactg ccagctatat ttatcaattg atgatgttca aacaagcacg cgatgcttat
     9061 caaaaagaat ttgaaaaagc aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc
     9121 tcttagttga aacgtgggtc gatgtctatt cgatgttgac cgacgttttt tctatgcctt
     9181 cagatttggt tttaagcgat atctgtttag atgacatttt gcaaatggct tacaacaaga
     9241 gtgcttatga aggatggaaa aactatgcaa taaaccaatc ccagaaaaac taaagaaagg
     9301 aggtaaaaaa tggctaaaaa gagaacagaa gcagaagtaa cattcatagc taacgatgac
     9361 ggattgaaat ctacgttaaa agaaatcagt gctgaattaa ctaaaaatag agcagaatta
     9421 aaactagaac aagctcaatt acaacagact ggttctgaat cagacaagtt aggaagtaaa
     9481 ttatcttctt tagaaaagca gtatgaatta caaagtcaaa aagttgaagt aactagccaa
     9541 cgtttagcca atgccaaaaa atattatgga gaaaattcca ccgaagttca gaaacttgag
     9601 agagaactga ttaaccaaca aacagcgcaa caacgtttgt caaacgaaat tgataaaacg
     9661 agtaatgcac tagctcaagc aaaaggcgaa atacagacgt acgagtctac aatgcaacag
     9721 ttggatagtg aacaaaaaaa tgttcaagct agtgcttctc tgattgaatc cgaatacaaa
     9781 aaatggcaag caactgctgg tcaatcagct tctgaatccg agaaattagc gaaagcccaa
     9841 gaatatgttt ctcaacaatc tgaaaatgca gagaaaacga tagatatcct gagacgacag
     9901 ttagaagcta cacagtctga gtttggcgct acatccacag aagcaatgca gatggaggcg
     9961 aagcttaatg atgctgaacg tgaatttgaa gagttaggac aagctgctaa aaatgtagat
    10021 acaactaact tggacgatat cggaagcaaa atagatatga ataatctaat ggaagcttct
    10081 gacgttttaa gcgacattgg cgataagctt acagaattag ggaaacaagc agtggactct
    10141 gctaatagtg taggtagttc ccagagtaaa atacaagcta attttggttt gactaaacaa
    10201 gaggctgaag aattaacgaa tgtagccaga gacatttatt ataaaggttt tggagaatcg
    10261 ttagatcagt ccacagatgc attgattttg gtaaagcgta atttaggcga tttaaataat
    10321 caagatttac aaaatatcac ggaacaagct atggtcctag aaaacaccat gggcgctgat
    10381 atggatgaaa cgttacgtgg tgtaaatggc ttaatggtca atttcggctt gagcgctcaa
    10441 gatgcaatgg atttaatggt ttcgggtact caaaacggtt tagataaaac gcacgaatta
    10501 ggcgacaata tggcagaata tagccaatta tggagtcaaa tgggatattc agctgatgaa
    10561 acgttcggaa tgcttcaaaa tggtttagat gcgggtgctt ataaccttga taaagtcaat
    10621 gacttagtta aggaaatggg aatatcgtta acagatggtc gatttgagca aaacatggat
    10681 atgtttagtg aaagtactag aaaagctttt gaagagtgga aaaatggcgg aggaacacaa
    10741 aaagacgtta ttaattccat gattcaagat tttagcaata tggatggtca atacgaccaa
    10801 ttaaataaag cttctacaat ttggtctgca cttggcgaag ataacgcgat gaaagttgtc
    10861 caatctttga ctgatgttaa ccatacattt gatgatgtta gtggatctgc acaaaaaatg
    10921 aatgaagatt ctactactcc gttacaagaa ttaaacggaa aaatagctga attaaaggat
    10981 tcattagctc ctataggcaa cacaatcata gatgcactcg aaccagtaat tgattttcta
    11041 ggaaagatgg ctgatgcgtt taataatctt ccacaaccag tacaggatta tgccgtagcg
    11101 attggcggat tgactgctgc atttacttta ttaatgccaa taatagttgg cttcatggct
    11161 ctaggtggtc ctactacatt aataatagga gcagttatta ctgttattgc tggagttata
    11221 gcaattataa aaaactgggg cgcaattact gactggttta agggaatatg gagtaaattc
    11281 actgattggt tgggtggtac ttgggaaagt ataaaagaag gtgcctcatc agtttgggat
    11341 ggagttaaag aaacctggtc tggatttgta gattgggttc aagatatttg gcaaggagtt
    11401 tctgattggt ttggagagct atggagcgga ttagttgaag gagcttccaa catctggcaa
    11461 ggagtccaag agacttggca agcattcgtt gattgggttt caaatatttg gaacggagtc
    11521 aaagaagtat ggtcgattat ttgggcagac attgtaggaa ttgttcaaat accatggacc
    11581 ttaataacgt cattgattca agccggtatt aatattatcg tgggtatttt tgatgtagct
    11641 ggacagttat taggcgcagc ttggcaagct gtttggacac ctatttctga tttccttaaa
    11701 aacacttggg atactatgac acaatgggta agcatcgctt ggaatggaat tgtaactaca
    11761 ttccatacta tatttgatcc agtagtggca tggtggaatg gtatatggac agctattagt
    11821 actacggctt caaatatttg gaattcaatt agtgcaacag cttctagtat ttggaacagt
    11881 atcaagaata caatcactag cttggtacaa gcagctgcta cagtaattca aaatatttgg
    11941 tcaactgtat ctagttggtt aggtggaatt tggaattcaa tcagctctac agcatcaaat
    12001 atctggaata gcgtgactag tagtataagc aatgctataa acgcagctaa aagtgccatt
    12061 caaagtgttt ggaatagtat atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac
    12121 actgctttga atctttggaa tggaattaca agcactatta gctctaaagt aaacgatgga
    12181 aaaaatgcaa tttcaagcgg ttggtccaat ctaacaggta ttgtttccga catattcaat
    12241 aatgttaaaa gtacaattgc taacatttgg gaaggtatca aaaagactgt tagcgctcca
    12301 attgattgga tcagagataa aatcagtagt atctttgata atttgaatat ttctatacca
    12361 catattccgt taccacattt taaattgagc ggagaattca atccattgaa ggggaaaatc
    12421 ccaacgttgg gtgttgattg gtatgcgaaa ggtagtgtgt ttaattctcc gaatattatc
    12481 ggtgtcggcg aagcaggacc tgaagcagtt ttacctttga aaagatctgt gctgcaagaa
    12541 attggtgatc gtatcttgag tagcacatca gtttcatcta gggcacaaac gattcaacct
    12601 gtgaataact acgaattcaa tttcacaatt gatggtaacg cagatgaggt tactatgaag
    12661 caaacaactc aacaaatcat tgatagcatt acaaaagttc aaaatgataa tgcttcggca
    12721 tggcgttaaa caggagagta tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa
    12781 tgactgattg tatacattct ataatcgatg gatttcctga ttatttgcat aaattggctt
    12841 tagctgaaag accaaccata ccttctccaa aaagacagag agttgaaact tctgttttag
    12901 gaaggttagg tggcttagta caagattact cgtttgaaga catgtcgttt acattgcact
    12961 ataactattt agaggatgtg gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc
    13021 gtcattggtt aaattatgca aagaaattag aattctctga tgatcccaac gtctattatg
    13081 ttatccagac tatcgatatt ggggatgcag aaaacgatat tgttgaatgg ggagagttcg
    13141 atgtaaatat tactgcgaaa ccattcgcaa gagttcaaga agatgtacct ataaccgtag
    13201 ataaaccaca gtcatttaac ttgctgaata atagtttaga agaaagtttt ccaaagatta
    13261 tcatcactcc ttcagctact tcatgccagt tcatcttaaa tgattatgtg tttagttttg
    13321 aaggcttagt agtaggaact gacgtagtca ttgatagtga tttgatgctt tgctacgaag
    13381 agcaatcgga cggagatatt ttagatcggt ccaacaaaat gaagaccatg caatatccga
    13441 cattgcaagt ggatattaat tattttaatt gtactggttt gagcaaaata caaatttatc
    13501 gtaatgggtt aaggtaggtg aaatagatga tcgataattt aataactatt tacgataaaa
    13561 acgacgcgaa taatttagct gaacatttat atgatacgca aggtttaggc gctttgtcag
    13621 actggttaac agctactgtt agcaataaat taaacggagc cgagatattt cagggtactt
    13681 atccaataag cggaactaat gcagatttga ttgtagaagg acgtattatt cagtgttatg
    13741 tagatgaaaa tcgagcaaaa cagcgtctac ggatttatta tgcaaagact tctgtaatag
    13801 gcaatacgat agaagtaaaa gctgaaccta ttttcaatga tataagaaaa tcggtgttga
    13861 ataaatatga cagtggaaca gaaaagatca ctgctagtca ggcatggcaa aacgcaaaaa
    13921 ctttagcgaa accagttatt ccttcacagt tttctttctc gtcattagta gatacgcttg
    13981 ctaatgtgaa gatagaaaag gcgaattttt tagaattctt tggtggaaaa gagggatcta
    14041 ttctagatcg atttcatggg gaatttctaa aagataataa cacattacgt catgaaaaaa
    14101 ggctaggcac ggatcataaa atcaaagcga tttatactaa aaacttaact ggtcttgact
    14161 tagagattga tgctcaaagt gttttagttg gagtttatcc attcattagc agctcttcag
    14221 aaggagaaga cgagatcact ctaccagaag aagttatttt cacggattac gtggatgatt
    14281 atcctgctgg atatgtttct tttgttgatt ttaaagacaa agcgactgat gtagccacat
    14341 taagggaagc tgctaaagac tggttgaaaa caaacataga taaacaaaaa ccacaagtga
    14401 gtggttcgat tgaattagta ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc
    14461 tagaaaaagt ttcgatgggt gacggagtag atgtgtatca tccacagtta aaagtgaata
    14521 tgtcagcgag aattgtggaa tatacgttta atgttctaac caattcatac gataaattag
    14581 ttgtaggaaa cgtcaaaaca aacttcttag aaaacacaga gaataatgtc agcaatttga
    14641 ttaatgatgc cattgatcaa ttgaaaaacg gtggcgaaat cagtgattta ctcaatgata
    14701 ttgtagatca tcaaactgat atgattactg gtcaaaatgg tggttatgtt ttattagatc
    14761 ctaaagaagc gcctagtcgt attttgatta tggacacacc agataagaat accgcaagga
    14821 atgttttaca aatcaacaat gctggtattg gtttttctaa aactggcatt aatggaacat
    14881 atgaaacggc atggacgtta gatggcggat tcaatgcctc gtttattacg gctggtgaaa
    14941 tagtaggaat tactattaga ggtactacat taattagtga tggtgctgat tatagaacaa
    15001 gtattgctaa tggcaaaatg acttggtact caaaaaaagt taacaaagat attatggagc
    15061 tagaagcacg tgattatgta agtgctgatg ccggtattgt atcatacacc atgaaaactg
    15121 gtggtggttt catgattaga aatccacagg gtaacttggt ttttagtacg tgggataatg
    15181 gtaataacag accgtttcta tcttttggtg cgcccaattt caggtatagc aatgctagtt
    15241 atgtaacttc tggcgacggt agttctttaa gcattaatgg tagtgcgggt aactcatggg
    15301 aatttaaggt agctggtagg actatgaaat ttactagtga tggtatgcta acgttaccag
    15361 gttgtttttt tggttcatgg gaagatggga aacttgctag gtttgaacaa tcaacggtac
    15421 aagtatataa agattttact gttagaggta ctaaaaactc aactgtacca acagaacatt
    15481 atggacaacg actattgaac gcttatgaaa ctccagaata ttatttcgct gattatgggg
    15541 aagccgttac aggtgacgat ggtaaagttc gtgttgatat tgaccccatg tttgctgaaa
    15601 cagtaaatct aagtcggtat atgacacatg tgacacctac agaactagtt ttgtgtgctg
    15661 ttactcatga agatattgac catttcatca ttgaaactag taagccaaac gtattagtta
    15721 gatggaattt agtggcacac cgtctagggt atgaagatat tagattaaaa gaggatacag
    15781 catatgatag cacagtgctt gaccaaaaac gtttttaaaa cgaagacaag gaggtatata
    15841 aatggctagc agtttatata atttggcttt agatttcagc aaagaattaa actacaccaa
    15901 agctattatg gctcgtcaag gtgataaagg gattacggtg acggttaaac cgtttctaaa
    15961 tggcttgcag atggatacga gtggcggaac atttacttta aaaggaacaa caccatctaa
    16021 ccgttacgta gataatgttg caactagtgt aactagtgaa gaagtcacgt tttctcttga
    16081 tggcacattt atgagtgaag caggatatta taaacactgc tacgtagaat atagaaaaga
    16141 caatcaaatt ttaacaacgc aagatatcat ttttttctca ctaggagtgt ctgacatttc
    16201 gcaaggccaa gccgatgaat atgtttcgca attagaagag ttgattcgaa agtacaacga
    16261 aacttttgat gcttttatgg ctgaaatcaa aggtagagtg gatagcttaa atcaacagat
    16321 tactgattta actggtcaag ctaaaacgct acaagacaag ttagatgctc tgaaagaaga
    16381 aatttctaag ttaggtaact tacaagtgat gtaca
//
LOCUS       DACEMO010000045        16196 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEMO010000045 DACEMO010000000
VERSION     DACEMO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.046"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(20..904)
                     /gene="rsmA"
                     /locus_tag="IW728_001112"
     CDS             complement(20..904)
                     /gene="rsmA"
                     /locus_tag="IW728_001112"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1024486.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="IW728_001113"
     CDS             complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="IW728_001113"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1024487.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(1459..2235)
                     /locus_tag="IW728_001114"
     CDS             complement(1459..2235)
                     /locus_tag="IW728_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1024488.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(2390..3712)
                     /locus_tag="IW728_001115"
     CDS             complement(2390..3712)
                     /locus_tag="IW728_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1024489.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            3887..4474
                     /locus_tag="IW728_001116"
     CDS             3887..4474
                     /locus_tag="IW728_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024490.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(4953..6962)
                     /gene="metG"
                     /locus_tag="IW728_001117"
     CDS             complement(4953..6962)
                     /gene="metG"
                     /locus_tag="IW728_001117"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1024491.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            7230..7508
                     /locus_tag="IW728_001118"
     CDS             7230..7508
                     /locus_tag="IW728_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024492.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            7605..8426
                     /locus_tag="IW728_001119"
     CDS             7605..8426
                     /locus_tag="IW728_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1024493.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8711..9721)
                     /locus_tag="IW728_001120"
     CDS             complement(8711..9721)
                     /locus_tag="IW728_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1024494.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(9733..10686)
                     /locus_tag="IW728_001121"
     CDS             complement(9733..10686)
                     /locus_tag="IW728_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1024495.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(10676..11746)
                     /locus_tag="IW728_001122"
     CDS             complement(10676..11746)
                     /locus_tag="IW728_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024496.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(11752..12795)
                     /locus_tag="IW728_001123"
     CDS             complement(11752..12795)
                     /locus_tag="IW728_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024497.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(12796..13737)
                     /locus_tag="IW728_001124"
     CDS             complement(12796..13737)
                     /locus_tag="IW728_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024498.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            13821..14168
                     /locus_tag="IW728_001125"
     CDS             13821..14168
                     /locus_tag="IW728_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1024499.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(14238..15899)
                     /locus_tag="IW728_001126"
     CDS             complement(14238..15899)
                     /locus_tag="IW728_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1024500.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         4580 a         3329 c         2897 g         5390 t
ORIGIN      
        1 tctgtcacaa cctctttttt tactgcatat tttctgacat cgcattgctt aatgcggcga
       61 attcttgtaa tgacaaagtc tccccacgac gttttggatc gattccagct gtttccaagc
      121 tttttgctaa ccatgctttt gtctggtcat ctttgccata cgaatgctgc aagttattcc
      181 aaagcgtttt tcttctttgc tgaaatgccg cttttgtcaa tcggaaaaat gctttttcgt
      241 ctgtcacttc gactgccggt atatctcttc ttgtcagttt caaaatcgct gaatctacat
      301 ttggctgagg gacaaatact gtttttggta cgatgaatgc taaactggct tccatataat
      361 attgaacagc aatcgataat gaaccataag ctttcgttcc tggttcagca gagatacgat
      421 cagctacttc tttttgcatc atgacgacta gttcatccac aaccaaatct gattcaagaa
      481 aatgcatcat gatcggtgtc gtaatatagt aaggtaaatt cgctacgact tttaacggaa
      541 gctcctcatg aaatgtttct ctaacagctg ttgacaaatc ggtctttaaa atatcttgat
      601 gtacaatagt cacattgtca tagggttgca tcgtatcagc caatacaggg atcaaacgat
      661 catcgatctc aaatgctacg acttgttttg catgcttggc taattgttcc gtcaatgcac
      721 cgattcctgg tccaacttcg atcacgttcg tatgttcatc tatttgcgca gtttctacga
      781 ttttccttaa aatatttggt tcagtcagaa aattttgtcc taaacttttt ttaaatgaaa
      841 aaccatgttt caataaaatc tcttttgttc tagagggtgt cgcaatttca cgatattcac
      901 tcatgttgtt cctccactat tttcattgct tgggcgagtt ctttcggcgt aatacgaaac
      961 atcttcaaac gtttttccaa ttgtttgccg ttcgtgtaac cgatgcgcaa ctcgtcacct
     1021 agtttttctc gtttttcttt tgcttgtgcc ccagctatta aaccatagtc taaaaggtct
     1081 tgtctagtga tttcagaaac agccgtttgg gaaatgtctg gcgtcacgac ctttttcaat
     1141 gcttctaaaa tagcagcatc acttgcatgc tcgacaccaa ggcttccctt tttctttggt
     1201 ttagcttgtg tgcgcggcaa aaaagcatgt tttgcctgag gaacagcttc cataatgatt
     1261 tttcttattt tttctcctga aaaatcagga tcggtaaaaa cgatcactcc acgagtctct
     1321 tgggcatgtt caatttgcat caggatctct tcattgattg cagaccctat tgtttctatc
     1381 gtatctgcat caactacttc ccgcagcctt cttgtatcgt cttttccttc aacgactata
     1441 atctcttgga tcttcatctt atccatgttc gcttagacca aataatcggt gcgcatttgt
     1501 ccatgtttgg gcagccactt cttctgtcgg aagattccga agttcagcaa tcttttctac
     1561 tgtataacga gtatagcctg gttcatttcg ctttcctcta aaaggaactg gtgccagata
     1621 aggggcatct gtttccacaa gtaatcgttc gattggtaca tttgctgcag cctcttggac
     1681 gtctaatgct tttttgaaag tgacgacacc gctaaatgaa atatgcatcc ctaagtcaag
     1741 aaatcgtttt gcccattcat aatcaccact gaaactatgc atgattccac cgatatctcg
     1801 aacatcttct tcttttagga tttgatacgt gtcttcaagt gcttctctag tatgaatact
     1861 gattggtagg ttcatttctt ttgcgatagc aatctgtctg cgaaatactt tatcttggac
     1921 ttctttcggg tcttccatcc aatgataatc caagccaatt tcccctaatg ctacaacctt
     1981 tggtaaagtc aattgttctt gtaatttatt ttcgatttct ttcgtataac tgccagcctc
     2041 tgtcggatgc cagccgataa tactgtatat atttggatag tcatggctta attgcaaaga
     2101 cttttcaatc gttggcgtat cgaagcccac gaccgccatt ttcgttaccc ccatttcttg
     2161 tgctcgctga atcgtttctg gaatatcttc attgaactgt tcagcattta aatgggtatg
     2221 agaatcaaat atcatgttta tctctccttt tcattcttac tttagcacat aagcactact
     2281 tgcagaagta gcaaaaatca aaatagaaga acttgtttta ttgaaataga aaaagcacga
     2341 agatctagag gacctagttc gtactttttc tgtctgttgt tgaaatattt tactcttctg
     2401 agcgtttttt ctttttttga taatagactg ggatacctat caaggtaaca cctataccga
     2461 tcaatgctaa tttcgtttgc gtcatcatcg tcatacctaa aatgaaaata gcaccgagaa
     2521 tcgcaataat cggaacgatt ggataaagtg gtactttata tggacgtggc aattccggcg
     2581 ctttttttct taaaataaag acaccgataa aaatcagtaa actgaatagc cacatcacaa
     2641 aaatcaacat gtctgttaaa aagtcgaaag aaccaaggct catcataata caagctactg
     2701 ctaactggaa aaccgcagga acataaggaa ccgtaaactt ctttgaaagc tttgtcagct
     2761 gtttactgaa aggtagctcg tcttctaaag ccatcgcata aggcacgcgg atcccagtta
     2821 gtgtataacc gttcaatgca ccgtataccg agatcaagat cccaatagtg accagctttc
     2881 caccgattcc tccaaaaatg acgtctgatg cttcagaagc tgcattcaaa ttaccggcaa
     2941 gatgatcaat cggcaaagtt ttcaaaaaaa caaaattgat caatagataa acgactgtta
     3001 caaaactcaa accaagaata attgctttag gcaaatcttt ttccggccgt ttcatttccc
     3061 ctgccattgc accgacacct aaccagccgt cataagcaaa caacgttgct aacaacgcac
     3121 tgctcaatcc ttcgacaaaa gtaacatctt ttccagcttc gattggaaat aactggacag
     3181 ttccttgtcc tggggtcaat aatccccata tgacaatcac agcaattggt attaatttaa
     3241 caatcaacgt cactgattgt acagaagcac cgacttttgt ccctaacaaa ttcaaccctg
     3301 tcacagaaac agcggtgatc actgcaatca acaaaagata atcggtagat aattgtaata
     3361 agttcgtcaa ttgtgtcgca aagataatcg ataatgcagc aatattagca ggaaagtaaa
     3421 tcaagctttg cgcccatcct aataaaaagc tgggcaattt tccatacgca gcttcaatat
     3481 attttacagc tccaccagtt tccgggattg ctgtagctaa ttcagccact gtcaatccag
     3541 cacagatcgt taagaaacca cccaaaagcc aagctagcaa tgtcaaactt gtagactgag
     3601 tactagcagt aacagcagca gttttgaaga agacgcctgc accgatcacg gttcccataa
     3661 cggtagctaa tgcgccaaac agattgattt ctcgttttag tttgcctttc attcccatcc
     3721 tctttcttct ttgaaatttg gtataatttt cagtataact gagaatattt taatttacca
     3781 ttcttttttc tccactttca gtgaaaaact ccctcttaag acggatgtat ttctttttta
     3841 tttttcgtat actaaaccta ccttttccac gaaggaggaa cagtttatgg attttaagcg
     3901 ttcttatccg aaattagcag attatttatc caaccgggtc gatttttccc gtaaagaaca
     3961 acagcagtcc cattggcatt ggttcacatt aattttattt ggattaatga ctgttgcaac
     4021 gttatcacaa aaactaatat tgctgacttt gttcatcgca atcgttgcat tgatcaaagg
     4081 tcctgtgatg atcttgtggg ggatgttgta ttcctttttg gttgggcttt ttccgcctat
     4141 cggtattttg ctttcagcga ttttcttttt attgaatatc ggaacattta caaagaactg
     4201 gcggatgacc ttagtcggga tctattttta tttctatcct tttggagtga tggcacttag
     4261 tgaagtcatg cattgggata atcattggtt tatagctggc tccttgcttt tagggttgat
     4321 cttgttacac gtgatgttga caaagctcta tcaacattat ggtatcggac gtacgatttt
     4381 ctggtatgtc ttttcgatcc catttgcact gcttactgct ctcttaccaa gccgtttgaa
     4441 gacaaaaata aaaggttacc ataaaatcaa gtgataggat ttttacaaaa taaagaaaac
     4501 aatcatcgaa gtcaaagccg atgattgttt ttttatctat attttcaatc acacatataa
     4561 gagcacaaac taattgaaca taaagtgata gaacttataa ttaacacatc aacgctactg
     4621 ttagatataa atatagatgt aataaagtca ttgcattcaa actttatgaa tgtaaggaca
     4681 ataaaaaaga gtaccagatg aatcaattaa aatctgcccg accctttatc attatgcttt
     4741 ttttctgttt ttacgattcc tattagtatt attctaaatt aacaaaaatc agcgaagctc
     4801 gtacttcttt ttctaggatt tattttggat agttctctgt ttctgtatta tatgattggc
     4861 ttcaagtaat aagaaggaat ttacgaaaat tgcttctcaa atttcggaaa aagggggtat
     4921 gacacaacta gtgtcatacc ccctttgaat ttctatgcga tttctgagcc atttggcatg
     4981 gattttggtg cttcgattac ttgtaaagag ccatctggtg cttctgcaga aaggatcatt
     5041 ccttgactga tttgtccacg catctttcgt ggtttcagat ttgctacaat gacgactttt
     5101 ttcccgatca attcgcttgg atcaggataa aattcagcga ttcctgacaa gatttgacgg
     5161 tcttggctgt ctccagcatc tagtcggaat tgcagcaact tatctgcccc ttctactttt
     5221 tgacagttga tgacttctgc tacttttagt tctacttttt caaatacatc aaatttgatt
     5281 tgtttttctt tcgttgaaac aagttctgtt tcttctggat cccatttgac tgtatcttcg
     5341 tttgtttgag taccttcaga cattttttct tgaataaaag caacttcttc ttccatatct
     5401 aaacgtggga agattggtgt accttttgca actacttttg ttcctgaagg aaattcgcca
     5461 aagtgtaatc cttcaagatt cattgtttca gagtccaatc ctagttgatt gaagatcttc
     5521 tctggcgttt ctgtcatgat tggctgaagt aatatcgctg cgatacgcaa agattcagct
     5581 aaatggatca tgacactatt caattcagct ttcttttctt cgtcttttgc taatacccat
     5641 ggttctgttt catcaatgta tttgtttgca cgagaaatca gcacccagat ttcagccaat
     5701 gctgtattga attccatttt ttccattgca tcatggtaac gtccgacaac gttagcagcc
     5761 gtcgtagaca gttcactatc aaatggagtg acaagtgagg catagtcagg aacgagtcca
     5821 tcacaatatt tgttgatcat agcaatcgtt cgattcaata agttccctag atcatttgcc
     5881 agatcataat tgacacggga aacaaaatct tctggcgtaa agacaccatc tgacccaaac
     5941 ggaacagcac gaagcaaata ataacggagt gcatctaaac cataacgttc cacaagcatt
     6001 tcaggataaa cgacatttcc ttttgattta gacatttttc cgtctttcat caatagccag
     6061 ccatgcccaa atactttttt aggtagaggc aaatcaaggg ccatcagcat gattggccaa
     6121 tagatcgtat gaaagcgaac gatttcttta ccgaccatct gaacatctgc aggccagtat
     6181 ttttcgaata gactttcatc ttcggagcca tatcctagtg cagtaatata attggataat
     6241 gcatcgatcc atacatacac cacatgtttc ggatcactct tgactggtac tccccaagta
     6301 aaagttgttc gtgaaactgc aagatcctct aatcctggtt tgataaagtt gttgatcatt
     6361 tcgtttttac gagattctgg ctggatgaat tcgggatgtt cttcatagta ttccaataga
     6421 cggtctgcgt atttgctcat gcggaagaag tatgattctt cttttaccaa ttctacttcg
     6481 tgtccacttg gcgctttacc accgatcacg ttaccttcgt catctttgta cacttctgca
     6541 agttgtgttt ctgtaaagaa ttcttcgtca gaaacagagt accagccttc atattcacct
     6601 aagtaaatat ctccctgttc taataaacga tcaaatattt tttgaacagc tgctttatgg
     6661 taatcatctg tcgtgcgaat gaatttatca taactgatat caagcgtttt ccataatttc
     6721 ttgacatcag ccgccatttt atctacatat tcttgcggtt ttacacctag ttcttctgct
     6781 tttgtttcga ttttttgacc atgttcatcc actccagtga gataaaacac atcaaagccc
     6841 atcaaccgtt tataacgagc aattgcgtca catgcaatcg tagtatacga gttgccgata
     6901 tgaagtttgc cgcttggata atagattggc gtagtgatat aaaaagtttc cttttctgcc
     6961 acggggctct tcctccttta tcgcatgatg cgaattcgtt tccttgctag tataccataa
     7021 aaaaagcatg ctgttagctt tttttcaaaa tgaaaaagac tggataaacg acttctatct
     7081 acgttcaatc tatcctttaa aaggatgaat aggttgaata aatgcgggtt tttgttttgt
     7141 ttcatatcta ctaaaaaacg gctttttatt tctcccctca cacgtggtat aataaagact
     7201 atctatctta gtaagggact ggaacaatca tgcagaagaa acatcttttt tttacactta
     7261 gtattgcttt tctttcatta gcacatttaa tattcagcta tttttatatt cgtatgtatg
     7321 gttattttaa cctccatggg catttgaaca gttttatgac agcagcttgg attttgcgtt
     7381 ttatcattga cgtatatatc gttatttgtg gcttctttgc cattcgtgaa gagcggtata
     7441 aagtattgcc gttttatctg cttttctttt tattcaatct catacttcca tttatttttc
     7501 atatttaaat gataacctag atttttcaag taaatccttg gttttttttc agagtctgct
     7561 acaataaggt aaaaagtact tttactgtca atggaggtgg tcagatgaac cgttttgctt
     7621 ctaaagaaga agaaaaatta tattatgaac ataaagcaag tgaagaagaa tttcttaact
     7681 ggtatcaaaa acaggaacgt ccagaatatg acaagccctc tttgactgtc gatatcgtac
     7741 tgatgtgcta taacaaagaa gaagatcagc tgaaagtttt gttgatccaa agaaaagggc
     7801 atccttatcg aaattcatgg gcattgcctg gtggctttgt ccaaaaggat gagtcaactg
     7861 gagaaagcgt cttaagagaa acaaaggaag aaactggtgt ggttatttca aaagaaaata
     7921 ttgagcaact gcatacattc agtacgccta atcgagatcc gcgaggctgg gtcgtcacag
     7981 tcagctactt agcctttatt ggagaagaac cgctaatcgc cggagatgat gccaaggaag
     8041 tacgctggtt cacactagaa cgtcatagta acaagatcca tttatctagc ggagaagtgg
     8101 ctatctcgct tgatttagtc actggagagt cttctggcaa agatactttg gcttttgacc
     8161 atagtcaaat catcttgaaa gcattcaacc gtgtagtcaa taaaatggaa cacgagccgc
     8221 aagttctaca agttttaggt aaagatttca cgattacgga agctcgaaaa gtatttgcta
     8281 aattcttagg gatcgattat aaaacgatcg atcattccaa ttttaagaaa gctatgctcc
     8341 aatatttcga tgagatcggt gaacgtcctg taggaatcgg ccgtccttca aaaatctatc
     8401 agctcaagcc ggggcaccat gaataaggtt gtgagcaaaa gattcagacc taatcattca
     8461 agaaaaaacg caaaaacaac tctttgtgag ttacgttttt tgtttcagaa ttttgatgta
     8521 ttcgtaaact ttctgaataa aaaattaaca atagcagaaa aagctcgtac gaactttttc
     8581 tgctattgtt ttattggttt gtcataatta gtgagctttt cttcaaaaga aaaattccta
     8641 gaaacaaaag tcattaagac tttttattaa caaggctaac cctaacgctc ctatcagttt
     8701 cattcatttt ttatcccttg ataaccgaat gtctgcccat tttttctact acttggctct
     8761 ttcttacgga attgctttct tctgctccgt cggtattgat caaaagaaat tttttattat
     8821 accattggga cttataacgc aattttgtta ttaatttatg ccagtcgtga atagaaaaat
     8881 cagcatgtgc ctcttctttt ggaaaagatt ctacaaataa gtagtcatat tgtttgacca
     8941 atttcccaga taattgttca agataatctt ctcgctggtt cttttgtttc agataaagat
     9001 ccattacttt tctcttttgt ttctgataat tgcttgcctg atctaacctg atctttctat
     9061 ggtgagcgga gcggactttc acattcaatt tccgctgtgc atgttgcagc ttttcttctg
     9121 tctggtgaaa ttttggtaaa gttacttcta gaggtcgaga tgtttggatc aatgtcttcg
     9181 gatgataggc gatcccgatc catttagcta aagattcttt ttttactggc gaaacatcac
     9241 aaagtagtgc gacataaaat tcttcattat atcgagcaga aatcgtagcg gagcgaatcg
     9301 atccttcaac tggccgatgc tgatttatct tgattagttc tttttgtttt ggtaacttta
     9361 agtagccatt ttccagatag atcgtacggg tttggttgtt ggtcgtataa gattgccaca
     9421 tacttttttt cgttttaagc tttgggaagc tggcacgtcc cttgaaataa ttgcgaaaag
     9481 cccgatccaa attaagctgt gcatttgcta aagcaagact gtctacttct tttaagaatg
     9541 ggtattcttt ttttaatgta gcgggtgtga gcgaaaaatt ggctactcct gtttgttgcc
     9601 aacttttctg ccgctttttc agcaaatggt tataggtaaa tcgagcacat ccaaaagtac
     9661 gaatcaattg ctgtttttgt tcttctgtcg gatacatacg gaatttaaaa gcttttagca
     9721 tctatgtccc tcctagttag caaccagaag agattccttt tgtcgtttta gtttttttgc
     9781 gtaatatggg acttcctgct cagttgcata gacaaaatgt tctggtgcga tttcacgaag
     9841 ttgacgtact tgtccgtcat ctggttcagg ttgatatttg attcttctgc gttttctctc
     9901 atggtctgga tctggtagag gaatagcaga taacaaactt tctgtataag gatgaacacc
     9961 gtgattatag atttcatcac ttgttcccat ctctaataac aagccgttat gcattacacc
    10021 gattcggtca ctaatatgtt taaccatcga caaatcatgt gcaataaata gataggtcaa
    10081 ttgatgttct ttttggagat cttgcaacaa attgaccact tgggcttgga tcgacacatc
    10141 tagtgcagaa attggttcat cacaaatgat aaaacgcggt tttactgcca atgctcgtgc
    10201 aataccaata cgctggcgtt gcccacctga aaattcatga ggataacgag taccgtggct
    10261 tggattcaat ccaacagttc tcaatagatc atcgactttt tttgcacgct cttcagatga
    10321 tttagctagt ccattaacat caattccttc ggcaatgata tctctgactt tcatacgagg
    10381 attcaacgaa gcgtatggat cttgaaagat catctgaaca tcatgccgga atttcgtcaa
    10441 acctttttta tctttgatct tcgttatatc ttgaccgtcg aataatatct caccatcagt
    10501 aggttcattc aagcgaatga tcgtacggcc agtagtggat tttccgcttc cagattctcc
    10561 aactaaaccg aatgtttctc cttcataaat gtgaaagctg atgtcattta cagcatgtac
    10621 ttcattcttt tttcctacat tgaaatactg tttcaatcct ttaacttcaa gtagtttatt
    10681 tggcatcagc aattcctcct tgaccgtaag tatgtttttg tttctcttta aagatagccc
    10741 agcgacgttg gatttcttct ggcggtgtca ctttgggtgc ctgcggagca agtagccaag
    10801 ttgctgcctt gtgtgttttt gatagttcaa aaaatggtgg ttcttgttct gcatcgattt
    10861 tcatcgcgaa ttcatttctt ggatagaatg cgtctccttt tggcgggttc aataaatccg
    10921 gtggagaacc aggaatagca tacaatcgat cgttcgcact atctaatgtc ggcattgaac
    10981 caagcagtcc ccaagtatag ggatgttgcg ggttgtagaa aatttcttcc gatgttccaa
    11041 cttcaacgat tcttcctcca tacatgacag cgacacgatc agcgacattc gctactacac
    11101 caaggtcatg agtgataaag ataattgatg tatcgatttt tgtctgaatt tctttcagca
    11161 gctccaaaat ctgtgcttga atagtcacat caagcgctgt tgtaggttca tcagcaatca
    11221 ggatttccgg ataacatacc agtgcaatcg cgatcacgat ccgttgacgc tgacctcctg
    11281 agaactgatg cggataactg ttgatacgtt tctcagcttc gggtattcca actaatttaa
    11341 gcaattctaa tgcagctttt tgtccctctt tttttgaaac tttattatgc tttctaaggg
    11401 actcagcgac ttgcttccca atgggcatcg tcggatctaa agaggtcatt ggatcttgga
    11461 aaatcatcgc tatttcttta ccacggattt tttgcatctc tctttctgat tttccaacga
    11521 tatcgttacc tttaaagagg atttgaccag catctatatt ggcgttgctt gacagcaagc
    11581 gcatgatgct tctggtcgtc actgattttc cacttccaga ttccccaacg atcgccaatg
    11641 tttctccttt attcaaatga aaattgacac cgcgaatcgc tcgtaccttt ccagcatacg
    11701 tatcaaaaga gatttctaag tctttcactt ctaaaatttt cgtcatcgtc ttcactcttt
    11761 cattttagga tcaaaggcat cacgcaatcc atcagcaagt aagttaaagc caatcatgat
    11821 aaccgataaa gttactgccg gaatccataa tagataaggc agatacagga atgtcttgta
    11881 cccttcgctc aacatcgttc ctaatgaagc tgacggaggc gtaagaccta aaccgataaa
    11941 gcttaaaaag gcttcgaaga aaatagcaga tggaatacta aacatcattt gaataataat
    12001 gacactagag atattcggca aaatatgttt gaatgcaatt ttcattcttg attcacctaa
    12061 tgtcaatcca gctaaaacat attcttgatc ttttaatttc attgtttgtg cacgaacgat
    12121 acgtgccatt ggtatccagt ttgtgatggc catagcagcc acgattgaaa aaattcctgg
    12181 ttcaaaaacg accaacatca aaatcatgac aacgaggttt ggaattcctg ataagatttc
    12241 caaaaaacgt tgcatcacgt tatcgacacg tccgcctaac aaaccagaaa ccaacccgta
    12301 agctacacca atcgttacat ctagtaatgc agcaataaat gcgatcagca acgagattct
    12361 cattcccata aataagcggc ttaatacatc acgtcccaaa ccgtctgttc ctaaaaagaa
    12421 atttacgttt gaagggacgt ttgcttgagc atatttatca acaagttcac caccaaccat
    12481 tgcttttcca ttcaatccat cgatattgat tcccggaata cgaggaggca aattgatata
    12541 agccacgttt tgggcagttg gatcatgagg agaaacaaag attgtgatca cagagatcaa
    12601 aatgatgacc aatagtaaaa ccattgaaat aacggcagct ttgtttttct ttaaacggcg
    12661 ccatgaatct tggataaaat tcaaagaagg tgcagtaatc tgttcacgtt cttcttcggt
    12721 atttttttgt aatggaagaa attcatccgc agggatatct gcaactgttt cataacgctt
    12781 tggtatcatt tccatttatc ctcggctccc ttctgaaaca cgaattcttg gatcaactag
    12841 accgtacaat agatccacga tcaaaataac aaaaatcagc ataaatgagt aaaggatcgt
    12901 tacagccatg atcgtaggat aatcatttgt tgtgattgac ttaacgaatt gttccccgat
    12961 accaggaata gcaaagatat tttctaccac cagtgatccg gtcatcaatg ctacagctaa
    13021 cggtcctaat aaagtcatca atggaatcaa actatttcga agaccatgtt taaaggcgat
    13081 ttcccaacgg ctcaatcctt ttgcttttgc taattccaca taatcactat gcagtacttc
    13141 caccatttcc gtacggataa accgagcaga gtcagcaagc ggcgacatgg ccaacgcaat
    13201 tgttggtaag attgtgtaca caaagccatc ccattttgcg attggcaaaa cttgtaattt
    13261 gattgcaaag atatattgta ataaaacagc aaatacaaag tttggaatgg aacgacctaa
    13321 tatcgctacg agtgtagaag atgtatctgc ccatgtattt tgcttcatgg cagagatcgt
    13381 tcctaaaatc gtccctagga ctgttccaaa gataattgcc tgtaacccta actgcaagga
    13441 tggtccgatt cttccagcta acaaatgtgc gactggctgg tttttaaatt ggaaggaaat
    13501 tccaaaatct ccttgaagca aattagaaag ataaatccca tattgtacga taactggttt
    13561 atccaaaccg acttgtttat ttagcatctc gattgtttca ggactcaaac gttcctgatt
    13621 cgtatatggc gtgcccggca gcaattgcat caagaaaaag gtgattgttg caattaacca
    13681 aagcgtgatg atcatgaaaa agacacgttt taatacatat ttgatataac tattcactaa
    13741 tgtttccccc tagcttttga cttctgacag atgtttcgct acattattag ataatgaacc
    13801 aaaaataaag gagcaatcaa atgaaaaaaa aatctatccc ctacgcagta gcttttctac
    13861 tgattctggt tattttaata aaaaatctca tcaaccattc ctttacactc attcaattat
    13921 ctaatgatct attcttatgg tcattgccgt ttctgattat cggcggtttt ttatgggtct
    13981 tttcttcggg tttcttcgat cattttcagc gatcggtcca tcttgcacga acaaggaacc
    14041 gaaagaaaaa accagaattt tcctcgctct cgtctgccag ctacggtatg tacagctttt
    14101 ggctgatcat tgccggaata ctaattgctc tttctgctat tttcatgctt ttttctttgt
    14161 taggataagt gaatatgaga acgaggttgc gacatttgcc gcagcctcgt tttttctcat
    14221 gctcacggta ttaacgatta ttcttcaacg tatgtccatt tgtaatccca tgaagcgcct
    14281 gccgcatggt gaacgatacc tttgattttt tcattcacta ggtgaccttc tgctttttgg
    14341 tacaccggaa cgacacccat atcatcagag atgatcttga ttgctttttg gaaatcatcc
    14401 caacgggctt gttcatcgcc agcgtctgtt gatgaagctg ctttaactgc ttcgtcatat
    14461 tctgcattgc tccagcgacc acggttgtat gaattacctg tgacaaacaa atcagtaaag
    14521 ctgcttgggt ctgcatagtc agcagcccat ccgcctagta ctgcgtcaaa atcacccgaa
    14581 gttgagcggt caagacgtac agagaatgga acaggtgttg gtttgactgt tacgccatcc
    14641 aagttttctt ggattgagtt ttgaacatat tcgattactt ttttcgatga atcatcatca
    14701 gaagccatca aatcaaattc taaagaatca acgcccaatt cttttttcgc tttttcccaa
    14761 tattcttttg ctttatcttt gtcataagaa actaattttc cagcttcttt tgcaaagtct
    14821 tcgttcgttg ttggactttt taccatatct gatgggatca atcctgtaga gacaactgat
    14881 ccgtcaccta gtacttgttt tactaaagct tcacgatcga tagaataaga aatagctttt
    14941 cttaggttca cattgttgaa tggtgaattc ttttcacgtt gattgaattc gatatagctt
    15001 gtccgcgctt ctttaacaga agtataagct ggatcgtttg cgttttgctg tgctaactca
    15061 ccagacaaga tcacgtcatc tgcttggcca tctttgaata agttcaatgc agtagaagat
    15121 tcttttacta cactgacttt gatttcagaa agttttactg tatctttgtc ccagtaatga
    15181 tcatttttct tataagtcca ttctgtatct gttcctggtc catcaaaatc ttctaatgtg
    15241 aatggtccat tataaacagc tttatcactt gcagatgcat aggcatcccc attttcttcc
    15301 actacggatt gtttttgtgg gaaaaatgat gggaaagcaa gcagatattg gaaatatggt
    15361 gtttgtttcg tcaatttgat ttctaattca tagtcaccaa ctgctttgat ccctagttct
    15421 gatttatctt tctttccagc tgtaatgtct tctgcatttt caactggcgc atacaggtaa
    15481 gcatattcag aagctgtagc tgggtctact gttcgttgcc agccataaac gtaatccgct
    15541 gctgtaacag gatcaccatt tgaccatttt gcgtcttctc gtaatttgat tttgtaagtt
    15601 aatccgtctt cactgacttc tgccaactcg ctagctcctg ccggctgtgg tttgctgtct
    15661 tcatcaagac ggtaaattcc ttcataaacg ttattcaatg ctgtaaaact gattgtatct
    15721 gttgcaactg ataagtcagc tgtaggcatt tcttgaggca cgactaagtt aaatacgcct
    15781 cctccatctg ctgaaccgct ttttgctgaa ctactgcttg tggctgaact gccgccataa
    15841 catccagcaa gtacaaggcc gcataccgca accaaaccaa atgtaaattt ctttttcatt
    15901 ttacgtaccc ctttattgga attttcagaa taattaatat tttctgaagt tgtttaagag
    15961 tttaatgaat aattattaaa aaatcaacac ttttttttgt ctttttcata aaataatgat
    16021 tttttcgtga ggtttcaagg aatttttcag aaaaaatagc tttgtacaag gttttccatg
    16081 gtcttccaaa agccattaaa taattttcgt tatattgtct attattgcca tacaaagata
    16141 aaaaagaata aacaaaaaga aactgtgaca aaagtcttgt cacagtccct tatctg
//
LOCUS       DACEMO010000046        16159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEMO010000046 DACEMO010000000
VERSION     DACEMO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.047"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..379)
                     /locus_tag="IW728_001127"
     CDS             complement(<1..379)
                     /locus_tag="IW728_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1024501.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVL"
     gene            complement(509..1219)
                     /gene="budA"
                     /locus_tag="IW728_001128"
     CDS             complement(509..1219)
                     /gene="budA"
                     /locus_tag="IW728_001128"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1024502.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1232..2896)
                     /gene="alsS"
                     /locus_tag="IW728_001129"
     CDS             complement(1232..2896)
                     /gene="alsS"
                     /locus_tag="IW728_001129"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1024503.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3101..3763)
                     /locus_tag="IW728_001130"
     CDS             complement(3101..3763)
                     /locus_tag="IW728_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1024504.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3773..4588)
                     /locus_tag="IW728_001131"
     CDS             complement(3773..4588)
                     /locus_tag="IW728_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1024505.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4850..5293)
                     /locus_tag="IW728_001132"
     CDS             complement(4850..5293)
                     /locus_tag="IW728_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1024506.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5427..5765)
                     /locus_tag="IW728_001133"
     CDS             complement(5427..5765)
                     /locus_tag="IW728_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1024507.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5753..6130)
                     /locus_tag="IW728_001134"
     CDS             complement(5753..6130)
                     /locus_tag="IW728_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1024508.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6354..7547
                     /locus_tag="IW728_001135"
     CDS             6354..7547
                     /locus_tag="IW728_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1024509.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7713..8135)
                     /locus_tag="IW728_001136"
     CDS             complement(7713..8135)
                     /locus_tag="IW728_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1024510.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8530..8724
                     /locus_tag="IW728_001137"
     CDS             8530..8724
                     /locus_tag="IW728_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024511.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            8721..9215
                     /locus_tag="IW728_001138"
     CDS             8721..9215
                     /locus_tag="IW728_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024512.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9377..10549)
                     /locus_tag="IW728_001139"
     CDS             complement(9377..10549)
                     /locus_tag="IW728_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1024513.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10755..11570)
                     /locus_tag="IW728_001140"
     CDS             complement(10755..11570)
                     /locus_tag="IW728_001140"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1024514.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11720..12166)
                     /locus_tag="IW728_001141"
     CDS             complement(11720..12166)
                     /locus_tag="IW728_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1024515.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12163..13167)
                     /locus_tag="IW728_001142"
     CDS             complement(12163..13167)
                     /locus_tag="IW728_001142"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1024516.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13207..13536)
                     /locus_tag="IW728_001143"
     CDS             complement(13207..13536)
                     /locus_tag="IW728_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1024517.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(13662..15989)
                     /locus_tag="IW728_001144"
     CDS             complement(13662..15989)
                     /locus_tag="IW728_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1024518.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4612 a         3228 c         2867 g         5452 t
ORIGIN      
        1 ctaaaacacc gattaaatta ccttctttta ccattggcac tacgatttct gattgtgcgg
       61 ctgaatcgca ggaaatgtaa tttgcatggg ttttgacatc atcaacgatc aatgtttctt
      121 gtttttcagc tgattctcca caaactcctt ttcccatctt gatacgtgtg caagaaactc
      181 gcccttggaa cggtcctaaa atcaattcat taccatcgaa taaataatat cctgcaaaga
      241 cagtctcagg caaagtctca ttcaacaatg cagatgaatt tgctaaattt gctaaccagt
      301 tatcttctgc ttctaataat ccttgttgct gtaataataa aatttcgtat gcatcttttt
      361 tctgtttttc tgtccacatg ataaaaaccg ctcctttttg atctatggag aagattatag
      421 ctcagttttt ttgtaaacgc taggtttcct cctcaaaaaa acgagatacg tcaggttaat
      481 caccttgaac gcatctcgct gtctttttct attctgcttg ttggatttct gctgctaaat
      541 cagcataatc aatcgtttcg tgaacaaaag tcggatcatc aactggaaaa tgctgtacaa
      601 gggacgagat gcggctaatc tcaacttttc cttcagccac ttcaaaatcc attacatgtc
      661 cgccaaatgt atgtgtatca tcgataaaat gcaagtgaaa tcccgctgca gcaactcctt
      721 ggaataattc aggcgtaaag aacccgacta atgttccttg gacattgctt tgttggaatt
      781 ctggttgaga ctctgaaatt tttgctagtc ttctgtaagg tttttcttgc ttcggcatga
      841 tacggatgtg catatttttg aataatccag ttatcttcac tgccaaaaat aggttttccc
      901 cggcttctct tactaatatg tcttttttca attgttctcc atctgcagtt tttgagacag
      961 aaaaactagt atctggcgta aaatctgtaa ttgctgcata gggggtaagt tcacttccat
     1021 ctaattgggc aactgagcca tctgatcttc cttgataagc tacgccatct aaaaagatga
     1081 cttcaccatc aagcccatgc aatgttccca atcccagagt tcctttttct aatatatgag
     1141 caatcgtttc tgttccatcc atcaagccag ccatcaaagc tcctaatgtc ccgtgctggt
     1201 ataagatttt ttcagtcatg tcgacacttc cttaatagaa ttgatctggt aagattgttt
     1261 ctcccaattt ctcattatca cggtaatcaa tcggaatatc aataattacg ggtccttctg
     1321 tcgcaaatcc ttcttctaaa gctttttcta attcctctac acttgtagct cggattcctt
     1381 tagcgccaaa agcttctgca tatttcacaa aatcaactgg accaaagtct acacctgacg
     1441 cccgctgata tttcattttt tcttggaact ctaccatatt gtagtgtcca tcattccaaa
     1501 tcaaatgaac gatattcaat tttttacgta ccgccgtttc taaatcctgg gcagagaata
     1561 aaaatccccc atcgccggaa actgatacga tttgtgtatt tggacgcacc aatgcagcag
     1621 aaatcgccca tggcaatgca acaccaagcg tttgcatacc attactgaat aacaaatgac
     1681 gaggttcata acttctaaag tggcgtgcca tccaaatata atgactacct acatctacgg
     1741 taacagtcat atcatctgtt acttttgact gaagcgtgtt aataacttct aaaggatgca
     1801 agatacctgc ttctcctttt gattgaacaa tgtcccgctc cgttaattct gcttgaagag
     1861 atgctaaata ttcttttgca tcttctgaca cttgttgagg ctctaatgaa cctgtcaata
     1921 aatcaagagt ggctgagata tcaccgatca attctctttc aggctgcata aaaggatcga
     1981 tttcagctgg cgcttcatcg atcacaatga tccgcgcatc tttttccgca ttccagtttc
     2041 ttgcttcata ttcgattgga tcatagccaa tagcaataac tagatcactt cgtttcaaaa
     2101 gcatatctcc tggttggttt ctaaataaac ccacacgacc aaagaaatgc gcttctaatt
     2161 cacgtgagat cactccagct gcttggaatg tctctacaac aggcaatgct gtctttccaa
     2221 ctaattgcct gatggctaac gtttcttttt cacttgatcc tcgcatacct actaatagta
     2281 ccggtagttt tgcttctcta atgcgttcag ctaggtaacg gatgtcatct gcagaagcag
     2341 aacctaattt aggatcgctc aatggcttga tgacattccc ttggacaggt gcatccacta
     2401 catcttgcgg gatactgata aagctcgcgc ctttttttga actcttagcc atacgataag
     2461 cattggcaat cacttctgaa attgtttctg gatcttggat ttctgcactg tattttgtga
     2521 ttggctggaa cagtgctgca ttatccatgc tttgatgggt caacttcaat agatcgcttc
     2581 gttttacttg tccggcaatt gctaaaactg ggtcaccttc tgctgtcgct gttaccaaac
     2641 ctgtagctaa gttactagca ccaggaccac ttgttgcgat aactactcct ggctctcctg
     2701 taatacgtcc gattgcttgt gccataaatg cagcattttg ttcgtgacga gtcacgatta
     2761 attcaggacc ttgatcctct aattcattaa atacaccatc gatttttgca cctggtatac
     2821 cgaatacata atcaacatga tgattaatca aactttctac tacagatttc gaaccttgtt
     2881 tcattttttc gtccattgaa catcgtcctt tttatgcttt atttttaatt attagttaga
     2941 aataatcaca aataattgat acagtttcta aacctgtttc attcaaaagt atagcacgtt
     3001 ttatttcatt tgtttagcca tatgtttcaa aaaatgaaaa agaatgacag tttttacata
     3061 aaaactgtca ttcttaattg aaagtttcaa tttataaagt ctacagatcc atagttctca
     3121 tcaatttacg attgttgaag taaatcaaac caaatacgat agaaggacct agtagaatac
     3181 accattgggg ctttacgaaa aagaagagaa acaaacttgt caatagtaaa aaatctagta
     3241 aactcacata ccattttttc aacgtatagt agaacgctat ccgtagaact tgatttggtt
     3301 tcgacgctgg atttctcacc tgaaaatacc agtagttgat gctaaaggaa actgataaac
     3361 aagcaagtaa aacaaacaac ggaatcaacc attttccata agaaaaacgt ataaaaaaga
     3421 aacaatcaaa aatcattatc acactaacta tccaagcaaa tagccaataa aaaaatccct
     3481 ttagccaaaa ttggcgaaac gtctttatga acaaacgtac tggtgaaaaa cttttttctt
     3541 ttgctaattc ttctgtcata cctatcatca cgatgcaacc tggaccaata aaaagtaaag
     3601 aaaaaataaa ggaaaagaca ttccttccat ctatcgacaa aaggaacgca gctgctatat
     3661 acaagatatt gacacaccaa aaacaaaggc tacttgccaa tgattgatac aaccaataaa
     3721 agaattccat ataaacattt ttgtcaaaac gctgctcttt cataatcacc tcctattcct
     3781 tcattcctga tgaagcgatc ccttctacaa aatatttttg catcgctaaa aatataaaag
     3841 caacaggaag aacagaaaca actgaggcag ccatgattaa tgcgtactct gcatcaaata
     3901 gcccaataaa cattttcaac cctaactgga ttgttttatt ttccgtagaa gacaaataaa
     3961 tgaatggccc catataatcg ttccatgtat taacgaatgt gatgatcgtc aaactggcaa
     4021 tcgccggttt tgtaagaggt aaaatgatct tgcgatagat tccccactct gataagccat
     4081 cgatccgcgc actctcacat aaagaatctg ggatactact gtagtattgc ttcaacaaga
     4141 acacaccaaa agcattgaac gcttgtaata gcaccaacga ccacaacgta tatgttaaac
     4201 ccacttttct catcataatg aactgcggga tcatataaga ctgccaaggg acagcaatcg
     4261 tgccgatata tgctaaaaat aaaatatcgc gccctttaaa atttagttta gaaaacccgt
     4321 aagcagcaaa actagaagta aacaactgaa tcaacgtgat cacaacgctt aaaaaagctg
     4381 tattcttaaa aaatgtcact aaaggaatac gagaccaaat aaccagataa ttctctaaat
     4441 gccaagtttt agggatccat tgaatcggaa tcgtgaacac ttcattattt gctttgaatg
     4501 aagcagaaac catccataaa aaaggaatga tcgtcaataa agctaataca atcagcagaa
     4561 tcatacccaa ccattgaata aagggcatgt tttttgaacg aatttttgct ttttttcttt
     4621 catttcgaac acctccaaag ttataatctt catttctttg taatgattac aatccagaac
     4681 aacggttatt atagctgatt catttccgtt cttcttatgt ttgtaagcgc ttatttaaca
     4741 agttatgtaa atctctttat tttttttgat ttaaaaatga aaaaatacaa aagcaagact
     4801 gtcatacgtc tcgcttttgt attgaaattt cttcgattag agatacttat tactgttcaa
     4861 aagcttcttt gaaacggtga atcaattcat tgactacagg tgataaaatc cgattctttt
     4921 tccagactag catgcagttc gtttcaatca ttgggtcaag cggaacgaat gtcactcgct
     4981 ttgaaagatg acttacagca ccttctatga ctaaagcagc acctacctga ttctcaatta
     5041 gcgcaaatac attgaaaatc aaattgtacg ttcctgtgat cagcaactgc tcctttttta
     5101 tgcctaacca gtcagcaaac attcgctgta tttctggacg ctttgagatc aaaagaggta
     5161 cattcgcaaa gtcttctttt gttatcacgt ctttttttgc taaaacagaa ttttctaaaa
     5221 tgagcaatcc ccatttttcc tttcttggta acaaaatcgt ttcgtatttc tctgtatcta
     5281 ctggatctag caaaatcgct aaatctaaca ggcttttttc tagtcactcc gtgatatcat
     5341 cacttgttcc gctgtggata tgaaattgaa cgctgggata atcacggatc aacttttcta
     5401 gtaatagtgc caatgtattc gaattatcag cttccacaca gccgatcgta aatcggccgc
     5461 tgaataattg tttttttctg ttctcgaatt cttgagtggt ttgattcgtt aaatctaaaa
     5521 tttcttctgc tcggcttttt tagaaaataa cctgcttctg tcaagatcat tttgcggttt
     5581 tcgcgtacaa acagttctgt tcctagttcc tcttctaact ccttcagttg tcggcttaag
     5641 gttggttgtg taagatgtag aacttctgcg gcttgagaga ttgttccttc ttctgcaatt
     5701 gtccaaaaat attttagtaa gcgtaactcc atagcgatgc tccttgattt ttttacttga
     5761 tacattccat tttatttcta gataacacac gatcatattt cttgattttt ccttgtaaac
     5821 gttccaaaca ctcttgcatt tcagcaattt ctttcgtcaa aagttcactt tcttcaatca
     5881 aaagcttacg ccgctcaaat acagttgctt ctccttcgct agataggcga atatattcaa
     5941 tcaaagactc gatcgataat ccggcatttc gcatacagat cgtaaagtcg acccatttta
     6001 ggtcttcttc tgtataatca cggattccgc cttttgtccg attaactggt ggaatcaacc
     6061 cgattcgttc ataatagcgc aatgtatcgg ccgaaatgcc cttttcttca ctgacttgtt
     6121 tgatattcat ctgatcactc tttctcttaa tttcttgcca ctttacttat gtattttatt
     6181 tattctacaa ctttaagtgt actccaagtc aatactcatt tctattaaaa ctaagctgga
     6241 ttatctatat cggttgtttg tgtattattt caaataaaaa agtcttgttt ttttattctt
     6301 ttttggatat acttttaaca atttagggaa attttaggaa aaggggtata aaaatgacaa
     6361 cgtatcagcg aaaggtatta ttatcgacat cggcgggaat tgctttagag aatatggata
     6421 ttatgttttt agctttttca ttatcttcaa tgattacttc ttttcatatt agtggtacgc
     6481 aagcgggttt catttcaacg atcactaatt tggggatgct cattggaggt attttttttg
     6541 gcatcatagc tgataaatat ggacgtgtga aagtattttc tcaaacggtc cttctgtttt
     6601 cgattgcttc tttattgatg tattttgctt caaacatcta cttggtttat ctctttcgct
     6661 ttatcgcagg tgtaggagcg ggaggagaat acggggcgtg tatgtccttg atttccgaaa
     6721 gtttttcaaa aaaacaaatt ggacgagctt cttcggttgc tggtattggt gcgcaggtcg
     6781 gtgcggcttt ggctgcgatt ttagctgctg tgatcattcc aacgtttggt tggaagatgt
     6841 tgtatgtcgt aggtgtactg ccggtgttga tggtcttgtt cattcgtcgc ggcttgactg
     6901 aaccaaagga attcaaatct gtacaatctg ccgaaaagaa aaccaaactt agcagactgt
     6961 ttgaatcaaa acatcttgct tggcaaacat gtggccttag tatcatggta actgtccaaa
     7021 ttgccggcta ttttgggtta atgaattggc tgccttcaat tatgcaaaat cgattagggc
     7081 tttcggtttc tgggtcttca ttgtggatgg tcagtactat tttaggcatg tctttgggga
     7141 tgatgacttt tggcataatc atggataaat tggggcctaa actagccttt tctttgtttt
     7201 taatttgttc atccttatct gtctatctac ttgtgttagc ccatagccag tggagtttgg
     7261 tagcagccgc tgtgatagtc ggctacttta tcaatggcat gtatggtgga tatggcgcag
     7321 tgattagtag tttgtatcca acagaaatcc gcgcgacggc gaataatttc attatgaacc
     7381 ttggacgagc tgttggtggt ttttcttcta tcgtcatcgg tttcttgatg gatcattata
     7441 gtttaactgc cgtaatcttg tttttgagcg gtatttacct agtcagtctg ctggtcttga
     7501 caactcttac tggtgttaca gcattaaggg aaagcttgca gcattgatag ttgagtctgt
     7561 aaatgacaag aaagaggtgt ggcaaaagct tgcatctgcg gtaataagac cagccaaatc
     7621 aaaaacatga gacactgttt ccgtttggct ggtcgtatta cttgatgcag ggcagctttt
     7681 tcaactgcta atttccttct atctggcggc gactattctt tgacgtccgt catttcaaag
     7741 tactttacta ataacttagc ataacgaaaa actgtggata cactcgtata ttgagcgtct
     7801 gcataatctt ccaaattttt ctcaggatgt tgatacatga attcaatcaa atgataatta
     7861 tatgattttc tgtagaaatg aagataaata atgtagattg ggaagttctc ctgcttttcc
     7921 atagaaaagc gccgattatt ctcattcttc aaaatgacct tatcaggata tttttggaac
     7981 aattcttcta aatctgttag ataattcaag atagacttac ttgatacctt caattcgttt
     8041 tgtagttctt ctattgaaca ccaacgttct gtttcaagca gtaaatgaat cattttaatt
     8101 ttcttttgag aaacttttgt taaaaaagtc tgcatatttt ctcctcctgt atctacttta
     8161 ctatactaca tacaagaaaa aaaacggata caaatatcat attgtcacac cgtcaatagt
     8221 gcataaacct gttattattt ctcttttttt cctaagtatt ctattgatat gaatcattta
     8281 atcgtttatt atataaatgt caataatcta ataataaaat aacgtacata ttttattatt
     8341 gcatacttaa taacagaact atagcaaaaa atcccgtgta tcaacacggg attagattaa
     8401 gtttcctcaa tcactattca taacacaatt attataacaa gtaagtaaac aattgaaaag
     8461 gacatttatt gaaaacggtt gttttttact taaggaggaa atttagcgaa aaaattagtt
     8521 gcattgtttt tggtcgcagg aagtttgttg agtgggtgtg gtgctactac gtctactact
     8581 caaagcacaa gttcagatca ggcttccagc agcagtacca cgatctccga aagtacggca
     8641 gattcatcga aagaacaaga acaaattgaa aagaaaaaag aagagaaaca aaaagcggaa
     8701 aaagctgcac tagaaaaaaa gtgacagaag cggaatctgc tttgtcacaa gctgaagcaa
     8761 atcctacaaa agaaaactat gcaactgctt cagcagctat ccaagcgcta cctgaaaaca
     8821 aacaagagtt gacaagccgt cttgctgcaa ttgattccac aatcaaagca aaagaagcag
     8881 aagaagaacg gaaaaagcaa gaagaaattg cacgacaaca agcagaacaa gcgcaacaag
     8941 aagcaatcgc acagcaacaa gcagaacagg cgaggcaaga agcaatcgca cagcaacaag
     9001 cagaacaggc gcgacagcaa tcagcagctg aacaggcctc tcagcaggca caagccgcag
     9061 ctcctgagca aaataatgaa caaactgtct atgtcacacc gaccggttca aaataccata
     9121 cacataaatg tggaaatggt acgtataccc ctgccacttt aagcgaagct caagctcgtg
     9181 gtttaacccc ttgctccaaa tgttatggaa actaattcaa aagttatact ataataataa
     9241 aattcttctg aaacataaaa aaatagaaga tggcgttcca gatagtaaac caaaaataac
     9301 agtttacacg tccatttcgt cagcttctct cttttttcat ttagtctgtt ccctctgctt
     9361 ttttggacat cttcattcat tctttctcaa tcaaatagta cacctctttt tcttttcgca
     9421 gtttatatct tccttttttc aatttcattg aaaactgttt cgaatgttta tgttttacat
     9481 ggattttaag caggagctcc tcatgttgca agcgatagtt cctgcctttt aaatcagaaa
     9541 atctcactag tccgtattca tcagaagctt tcacaaatgg aactttttct ctagtaatat
     9601 agtgtttgcc gttgagcgtt tttacagaaa agaccattcc ttcgattggt cgtccgtttg
     9661 tgttctttac ataaaaagat aaaggatagt ttcgcttagc gatggccaaa gaatctaaat
     9721 aagcgatgag tagccaaact aagccgattg atccaagtag tataccgata aaccaaataa
     9781 aaaatttcca ttttttccat tgatcaattc cttggatcgc tttttttgcc tcttttgctt
     9841 gataaggaat acgatgcccc gtcacaagca gtcgatgtgt attgaccata taaggagtac
     9901 aggtaagcaa agtcacgaga tctctgcctt tttggatacc caaagaatct acttcatcag
     9961 gtaagaccac ttggattttt tctacttgat acgccaatgt ctttccattg atctggatga
    10021 aaaactggtc tccttttttc aacttaggca aatctgtaaa taattttgct tctgggagtc
    10081 cacgatgccc agaaagaacg gcatgggtgt ttttcccgcc agtcggataa gaactttctt
    10141 ccaataatga cgttcctttt tgcaaaaata tttccgttgt ctgatcgaat ataggtaagc
    10201 tgacattgat ttttgggatt tcgatgactc caagcatatg ttccataaaa aaggcattac
    10261 taggtaaatc ttttaatgct tttgcatcaa ctgcttggtt gaaactgcct atccctggtg
    10321 atacgttttg ctctgccaac tgttggtttt tctttgtcat tttttcctgc tgttttttta
    10381 tttcctctga attcttattc gatgcttgct tttgataatg gatgatcatt tgctgggcaa
    10441 gaaaatcatt cagtgaatct tctacaaaag gataagaaaa gatcccgatc ccaacgacta
    10501 aaatgattgc catgaagatg tccagtattc ttattctgct tttattcatc atgtccacct
    10561 attttctaat atgtatatga agaggttgtg aaatcagcgc cttgacctga gtattagcga
    10621 agcaaacggc aaaatagttc ctcctatttt gccgtttgtt gagcgaatac caagagaccg
    10681 accacttgtc acaacctttc tccccatttg aatcactatc gtttatttat cgtatgattt
    10741 ttgtttccaa tagtttaagc ttctgtttct tgatggcgtt ttttgaaata gatcactgct
    10801 cctccaacta aagcagtacc taccaagatg aatacgatga tccccatacc accagttgaa
    10861 ggcaagattc ctttaggttg gttatcgatc gttgtttcat cagaagttgt tggatcatca
    10921 tctactgtaa attcagtgta cgcagcttca ttcttaggta atgcgtagcc ttctggtgct
    10981 tttgtttctt gaaggcagta tgccccttgt ttcagccctt cgattgaaaa tttcccttct
    11041 gaatcagaaa tcaatttcac ttcttcccag tcagaacctg tgacttcatc tgaccaagca
    11101 tatgggtatt ctcctgaggc aaggtttgct atttttttca ttaatttaac agttggatct
    11161 tttgctctta gaatagaaaa ttctgcatcg gctaattttt cttgcgtgtt cacatcttgt
    11221 ttgatgaagt ggaaatcacc gtagttttgg acattttcta cagtaatcgt gttatccgta
    11281 gttgtatttt cccgattgac tacaccgttt ggattcccaa gattggcttc atctgcgcca
    11341 tggatgatac cggttgtttg ttgcaatttt ccgtttttta ctataaaagt agtttctttt
    11401 tgtggattgg ttaattgata accatctggt gcttttgttt caactaaagt ataattgccg
    11461 tctattaatc cattgacact gatggatcct tcagtatcag ttgtgaatgt caacgctgac
    11521 tcagcaggat catagagagc gacaccagtg ttttcatcaa aaccggtcac gaattgattc
    11581 tctgaatttt tgatctggaa ttgcgcccct gctaacttat ctacccctga aaagttccca
    11641 gctttctgga ttttgatatt gctttctttg atgacatttt tagcatatag gtgaatcgtg
    11701 tctaaaggtt tgtcagggtc tagggtaagc ttaacacgat atttggcgac gtttcgacac
    11761 cgggctttgg tgtttctaca aagacgtata cagcatcttt cccatcagat ttacctggaa
    11821 gagtcaggtt agctaatcct tcaccagaag tcgttacact tgcgaagggt gtagcgtctg
    11881 ctggaactgc tgtgtcttta cttggatagt acgtagcagt gatgtcatat acgttgaatt
    11941 caacgccgtt caacggttcg ctattcggaa atgtcaattc cgttccgttg tttatttgat
    12001 tttctggtgc tgaagtgaat ttcaatttat gcaaattaac cgtaacattt tctggtgtag
    12061 gtgatacggc tgcagaaacc tctgcagttc ctaggccgag aacgaaagga agcagcatga
    12121 tagcagttcc taaaaacgtc catatttttg ttttgatgtt tttcatttta tttccctcct
    12181 attgtttcct ttttgttgtt ttttataaaa ccaagctgtc cctgttgaaa ggactagcaa
    12241 taaccctaaa attgagaaac ctccaagcat ctcttcgctt gtttttggaa agcttcctcc
    12301 atttttttca tttgatggac catttgtctg catctgaact ggcgtttttc cattattcgt
    12361 cggatttgct tttggttcgt tgatgatttc caatggtata ggatgaagcg gatcataact
    12421 gcctttttca acttcaaaga gaatggttga ttgactcaat atatatcctt taggcggttt
    12481 aatctccact agttcgtaac ttccaaaaga caatccatgg acagaaaatg ttccatcttt
    12541 actggaagta aaaatcgttg ctcttgaatc atctttggct acccaatgat ttatcccgtt
    12601 ttcttgcgct aagtattggt catttttttt gactagaaat tgagcatctg ctagtttttg
    12661 tttcgtttgc cgatccactt tgacaaattg tttgcctcct gtttggatgg caagatgtgt
    12721 ttctactgtt tgattgtcag tcgtcaaacg tacatcgtta tcaaatgtcg ttttttcact
    12781 gttttctttc aatgtcattt ggtaacggat cgttaatttt tttccagcaa acgaactaag
    12841 atcttttcca gaatctgata agacaaaacc atgttttatt ttttgtgtgt gaaatgtatg
    12901 aatcttgtct ccaccaatca aaaaatcgat cgtttctggt tctagccata actgaggatc
    12961 agctgtatct ttgatcgtat aggttcctat ctcgttgatg ttctcaggaa ttgttacggt
    13021 gatgagataa gggactttat ccccatctgc aaaatctgtc tgcttattct ctactgtttt
    13081 tgttaatgtg agtgcagcag gtattttttg ttcgctcttt ggatacaagt ggatggtcgt
    13141 caatttatga ttagatgaat ctgtcacagg aagcacaacg acaaatggtg ctgatttttc
    13201 ttttacttaa tctgaagtct ttatttccgt aaatcggtaa acagcgtcac gaccttgctc
    13261 atctttgtcc gatgcactaa aagaagcaat gccctcttcc tcgtctactg tttttgtcac
    13321 attttctgcc agtatttttt cactatcact tttttgtgct agttttcttt gtgcgtcttc
    13381 cacactactt ccttctgacc gcagcttgta aaattcatcc gagacatcat atatagcgaa
    13441 agtaacgccg ttcaaccctt ttactccttg aaagttatcc gggatattta ttccgtcgtt
    13501 ttgactgata cttggcatct tatcgctttc gaacattcga ttatgcaaag taaatgtaat
    13561 aggtgattct ctagctgaca cagtcggaga acctataaaa aagaccagta ggataaaaaa
    13621 gagaacagca aatatatcca cacgtacatt ttttgttttt ttcatcgctg ttcacctgct
    13681 tttttcttac gatggataaa ataaaagaag ccagaaaaaa taaagatcag tcctaaaaag
    13741 acaaagggca gtagacctat cccacctgtc gaaggaagca agagagtcgg attgatcgtc
    13801 aacttagcgg tatctgttgc gataatcgta taattattgt ttacacttcc ttcgcctaaa
    13861 ctaatcgtta ctatttgtga tccaatatct gtccggctgt taactgttag ggtataatcc
    13921 aatgattcgc ctaagacaac accggtaacg tggagatttc caaccgaaat cgagtgactt
    13981 tgggcatcat aattaaaggc agcgtcatcg acagtaatca ctgcgttagc tttatagatg
    14041 gtataagttc ctgatgtgtt ggctatatca gtaaatgaca acttgggatt tgctgctttg
    14101 actttgtcta ttccgctatt cgttagttta attgtatagc tgccagcatt aaccatattt
    14161 tttgctgatt gtgcatctgc tgtgtaatcc atactgctaa gtgccacttg tactggagta
    14221 agcgaactag tgacaccctc tgctagcttg acatggattg acgggacacc tgcttttcca
    14281 tcatatgtct tactgccact gagctctacc gcaccgctga tttgcttaat cgtatagaca
    14341 tcagatgtct gcacgatagt aaataaatag tttgttctgg cagctagttt agctagtccg
    14401 ctttttgtca attgccaaga cgtataggtt ccaatttcag taggcgcagt tacttcaact
    14461 cccttttcat tcatcgcttt gaaatcagtt ggattccatg tgatttgatc aatactgaga
    14521 tcattgttta aaagggatgt tgattctgtt ttatcattaa aggcatattt aacgatcggt
    14581 acaacagaaa gaccatcata gtacttactg ccatttgcat tgattgatac tgttgcattt
    14641 gtcttataat ataggatgac ttcagaaatc tgttttccat ttgcgtcctt tacgattaca
    14701 tttccatttg tatctaaact agctaaaaca ctcattgctg attggttctt tgtttctatc
    14761 gctgaaacag ctgtaaaggt atatccgttg atagtaggcg tattagtcgt caggtcttgt
    14821 gcagcacttc cgtaagtaag attcattatt ttagaaggag caatcatctg tccaaaatag
    14881 tcttgatact taatagtgat tggtgtttgt ttgcctgtat aggtcaccgt atcgacgtta
    14941 ctttctaggg ctgtgaaaga accgtttgtt tgtgcaaaag aatagcccgt tactgctgtt
    15001 ggagtgataa ccactttgtc acccacatgg attccagata atgtagtccc gtacgttccg
    15061 atttttgctg ctccaccatt gactgtataa gaaaaagttc ctgtccgatt cgcattgacc
    15121 tgaaccgttg ctgcttgata ggttggaaaa tttgaaacat ttcccttacc atcaacgatc
    15181 tgcccatttt tgatcgagta ccccgtgaat ggaatatcat ttgctaactg tataccgatt
    15241 gaaaaagtac tcatacctcc tccggcagga agccctcccc atgttgaggg tgtaaaagaa
    15301 taacttttat ccgcattggg tgtaactgaa tcgatcgtat ttccagcggt agccaccccc
    15361 atatagatac tttttgggaa tgtgatagta gtaccattta ctgtggagcc aataaacaga
    15421 ttatggatat ctggacggtt atcaataaaa gcgattgctt tggttacgcc ttgtaacgca
    15481 gttttatccg cactcatcga tccgttaacc gcaacaatga ctccatcttt ggcatatatc
    15541 actgtatatc catctggtat ggtatgtccg ttgaaattcg cagtggccgc aacagtacta
    15601 aatgtctggc ttctacctgt ttctgaataa gctttctccc ctgctgcttt tgctgctgtg
    15661 atttcttttt ctgtcggatc aatcagttga gtcgaggatg tggttgaggt tgtctcgttg
    15721 ttgctagact gattggtgct ttctatggac tgtatttcag aatagggtgg actatttgtc
    15781 tcctctgtcg tttggttagc aaaatttgac gtttctgtat aaacgtttac ataaatactt
    15841 gataagctcc ctaagaaagt aaaaatcaag atgatcgaaa tcataatggg atgtaagtat
    15901 tttcttttag atacagcttt gagtctagaa acaaaatgtt tttgacatat acgttggatt
    15961 ttattttgag ttttcttaat cattttcatc cctcctctca ataaaaaatc atcaaataat
    16021 cctttacacg ttattctaaa tttaattttt attattttat tgatgtatag aaagtttggg
    16081 gctttttttc tctttttatt ctaaggttat tttagtcttg tcctttaata caaagatttt
    16141 atttttaaat aaaaaatac
//
LOCUS       DACEMO010000047        16096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEMO010000047 DACEMO010000000
VERSION     DACEMO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.048"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(182..601)
                     /locus_tag="IW728_001145"
     CDS             complement(182..601)
                     /locus_tag="IW728_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1024519.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(603..1187)
                     /locus_tag="IW728_001146"
     CDS             complement(603..1187)
                     /locus_tag="IW728_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1024520.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1234..2709)
                     /locus_tag="IW728_001147"
     CDS             complement(1234..2709)
                     /locus_tag="IW728_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1024521.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            2890..3201
                     /locus_tag="IW728_001148"
     CDS             2890..3201
                     /locus_tag="IW728_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024522.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3316..5652)
                     /locus_tag="IW728_001149"
     CDS             complement(3316..5652)
                     /locus_tag="IW728_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1024523.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            5794..6462
                     /locus_tag="IW728_001150"
     CDS             5794..6462
                     /locus_tag="IW728_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1024524.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6465..7508
                     /locus_tag="IW728_001151"
     CDS             6465..7508
                     /locus_tag="IW728_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1024525.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(7745..8626)
                     /locus_tag="IW728_001152"
     CDS             complement(7745..8626)
                     /locus_tag="IW728_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1024526.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            8785..9513
                     /locus_tag="IW728_001153"
     CDS             8785..9513
                     /locus_tag="IW728_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1024527.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            9669..9986
                     /locus_tag="IW728_001154"
     CDS             9669..9986
                     /locus_tag="IW728_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1024528.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            9979..10587
                     /locus_tag="IW728_001155"
     CDS             9979..10587
                     /locus_tag="IW728_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1024529.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            10603..11433
                     /locus_tag="IW728_001156"
     CDS             10603..11433
                     /locus_tag="IW728_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1024530.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            11571..12059
                     /locus_tag="IW728_001157"
     CDS             11571..12059
                     /locus_tag="IW728_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1024531.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12110..12982
                     /locus_tag="IW728_001158"
     CDS             12110..12982
                     /locus_tag="IW728_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1024532.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13158..13520
                     /locus_tag="IW728_001159"
     CDS             13158..13520
                     /locus_tag="IW728_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1024533.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13529..14281
                     /locus_tag="IW728_001160"
     CDS             13529..14281
                     /locus_tag="IW728_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024534.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDVGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14295..>16096
                     /locus_tag="IW728_001161"
     CDS             14295..>16096
                     /locus_tag="IW728_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024535.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
BASE COUNT         5002 a         2803 c         3297 g         4994 t
ORIGIN      
        1 ccatgtttac aaaatttttc ttactactcg aaacgaagcg gctttatcac aacctcttga
       61 taaacggcgt tcaatcagct gtccctcggt attagctcaa aaacggcaaa atatgagaag
      121 ctatttttcc atttttttct ctcatactca ggtcataacg ctgatttcac aacctcttct
      181 ttcataatgg tttttcacca tctttataat aaactggttc tttgatatgg aaatctattt
      241 tttccaacca atctaaaatc ttgtaaccta actcttctga catcggaaaa gaggcgttga
      301 tttgtgcatt ggagatatgg tcaacatgga taccggaagt gatcgtacaa cttcccggaa
      361 cggcagtttt gatgatcaca gctatttttt cggctaagac atcgtcttta tggaaccgac
      421 catcgtggct agggaaacgt acagtttgca tctctgtatc acgagaaaat gtagtgactg
      481 ttccgatatg tggtgtatct ccgcctgtta aagtgatcaa taagtcttta ccgattactt
      541 taacagcagc ttggatcgtg aaacctagtt tggttacttc aactatcgtt tctttttcca
      601 tattaatcac cctgccaacg ttttgaatgc tcatttacaa tttcaaattg atccaaaagt
      661 ttcattgtat ggtgattaac aagatcttgg atcgtttgag gctgattata aaaagcagga
      721 attggtggta tgatttgcac acctagtcgt gctaatttag tcaagttttc caagtgaatg
      781 gcatttaatg gcgtttcacg aggaacaatg actaactttc gttgctcctt cattgttaca
      841 tcagcagcac gtgcaatcag gttgtcgcct aaaccaacag agatacctgc gactgttttc
      901 atacttgccg gtacgatgat cattccatcg gtcaaaaatg atccgctggc gacagcagca
      961 cctaaatctt tgtttgaata atggtaatca aacaggcttt cgaattcagt tagtgataag
     1021 tcggattcca gctttaagtt ctgttttgcc catggactga tgattccgtg tgtttctatc
     1081 atcggatatt cttttagttt cttcactagg ttgatggcat aaatggtgcc tgaagcgcca
     1141 cttacaccaa tcacgatttt tttcttccgt tgtgccaatt gttccatttt ccgcactcct
     1201 ttttatgatg tcaaaacagt tgcagatcat tcattatttc aaatattttt cccaatcttc
     1261 tacttcttgg aattttgcac gtacaaaatg ttctttcata tcgaaaggaa ctgtaccgtc
     1321 aaagatagtt tttgcactta tcccacggaa acgaatagag ccaggattat attctggtaa
     1381 ttctgatggg tccaatggat ggttcctcat tcctgggatc acgatgatat ctttatctcc
     1441 ttggaaacga gtgttgaaag tccaaactac atcgttcatg tcgaaaatat caacatcttc
     1501 atcgactaaa atgactgtct tcaattcttt gaaggctgat aaagcaagaa tagctgcttg
     1561 acgctgaata ccttcatccg cttcgttttc tttacggatt tgcatgatgg tcatcaattt
     1621 accaccacct gcaggaggat tgtagacatt tactactttt cctggaatag ctttatcgac
     1681 caaggcaaga atacttgctt ctgtaggaat accggccata gatacgtgtt cttcactggg
     1741 accaatcgtt gtttgcatga ttggattgtc ttttcggtga gtaactgctt tgactttcac
     1801 tacttgtaca gctggattag catccccagt atagccagga aattctggca ttgcttttcc
     1861 tgagttcgtg ttgatatctt cttgcattgt ttcatttggc atgatttctg cttcaatgat
     1921 gaattcagca cgagcaatac ccacttcatc gactgctata ccatcaacga tttgaatagg
     1981 ttcgttacgc aaagctcctg cacaccatag ttcattgtag ccaagtggag tggtaggtgg
     2041 ttcgaaagtc gtgccaatgg caattgctgg atctaaacca atattgatag tgataggcat
     2101 tggtttattc aaggcttcga attgtttttg gaagtgacca atgtggcgtc cgccaggcat
     2161 gatgtacata ccgatcgtat ctttatcttc tagaaccata cgatggatcg ttacatcgct
     2221 cattgttttt tctggatctg atcctaagac tacacccatt gtgatgtatg gaccagcatc
     2281 ttcaggagta tttgtaggtg caggcaaaat gttgcgaata tcgaagtctg catcggtagc
     2341 tagatgaacg acttcttgtg ctggagcgtc tgctttgtct actttcacag gtttgattgg
     2401 attttctaca gcgtctttca agaaacgacc aagtgttcga taatcatggt gcaaaatttc
     2461 acctacacgt ttacgactag ccattgttcc gatgttcaca cgtgtgccgg ggaaaccttt
     2521 gatattgttg aaagtcatag ctggtccttc ttgagtcgga cgttttactg taccgccagc
     2581 accgatataa cggtaaacac cagataattc tgcttcagga tcgatttcaa tatctgtttc
     2641 gtgatattga cctggaagtg tttttagttc ttcgatgact tttcttaaat catacggttg
     2701 atctgtcatg agagattcct ccttagttgt ctttacctat ctatagtaaa aggtgaaaaa
     2761 gaggaaaaca attcaacttg ttcggtaaag tattctacaa tggaatggat gcgttttttt
     2821 gataatagaa taaactatga tatacttatc actaaaagaa agcgcatact tagtgttgga
     2881 ggaaggaaag tgaaaaaaat ggaggagtca tttcctaaag cagtaaaagt ggaaaatata
     2941 gcgaatattt tgaaagttac atttgaaaac ggagaagtaa aatacgtgaa atctcattgg
     3001 acagaagaga taaccgatgc cttgcaattt ggaaaaaaag gaagaggaaa aagaaaaaat
     3061 cttctcgcac tttctcgtaa tatgtggatt gggactgaag taacgattga agcagatgga
     3121 acagtattca taaatggaaa agacaggtat acgccagaag agttgtggta caaaggaaag
     3181 aaaagtatac cagaattata aaaaataatt aaaaaaaaca gcgcgaaaca aggaacatat
     3241 tacttcctcg tttcttgctg ttttctacta tttcagcgct cttatgactc aaatctaaat
     3301 gtggttgtca caaccttatt ctgcacgcaa agcgatagcg gcgtcttttt tcgctgccat
     3361 tcgtgcagga agatgaccgc caagcatcgt caatatagta gagacgatca ctagaataat
     3421 cgcatggaca ggatttagtt gagcaacatt ttttagatcc gtcatgttgt ataagatgct
     3481 attgataggg aacgtagcta gccatgcaat aaacacacct aatatcccag aagaaatacc
     3541 taaaatacac gtttctgcgt caaatacgcg agtgatgtct tttttacgtg cacctaaagc
     3601 ttttagtaca ccgatttctt ttgtccgttc aatcacagat gtataagtaa tgatactgat
     3661 catgatcata cttgttacta aagagatccc tgcaaaagca ataaggacat aagtgatagc
     3721 atccatcaat ccgccagtca gttcagtcat cgttccagct aaatcagtat aaatgatttt
     3781 atcttcatct gatttgcctt tattgtaatc atctagataa tccaagatct tttctttgtc
     3841 ttcaaaattg ttcggataaa tcatgatact tgaaggcaag ctgtctccac ctaaataact
     3901 aagcaatgtt tgtttggcat tttcgtcaac tttttcagta gttaagacat ttacaccact
     3961 gtctctttga gctttgacga tttctgattc tttgttttcg ttgacgattt gagtcgttaa
     4021 ttgatcgcta taagcgattc ctggagataa gagattcatt gtagaagaag atttcacgcg
     4081 taggatcccc gagatcttca attcatcaga agcattctga tacatcgcat cataatctgt
     4141 attagggata aaattcccag ttggaagttt tgtatagtaa gtattgttgt ttactaattt
     4201 gaatgttgta ccaacgatat catcaaaatc aagtttttcg tcttctttca catcaaatcc
     4261 aagattcttc aatgcgttga tattagtatt attattgccg tctacgatca gcacgacatc
     4321 tgttgctgag gctggataac ttccggcaag taaagaataa ttgtctttta aaaagttttc
     4381 tttgctagtg tcaagctgtg tagggaaact tgacactccc acacctgtca ttgctgacat
     4441 cgtactagat aaagaaagag attccgtatc aggattttga ttcgagaagc tgacaggctg
     4501 tactttgcca tttacatcac gtaaaagatt gattcctgta gtacgggtaa atccgatatt
     4561 attacttaag ttaggatcga tgtctgttac gtaatcgacg tatgtttgat caatcttatt
     4621 cgtgtgctgt gctttatctt cttcgctgac ttttgcagta atcgttttgc tgtctggata
     4681 atccgctttg cttgcaccca atccggcatc gtcacgagtc tggttagtcg ttaccttaga
     4741 aatagtaatc ggaaatttcg ccatcgtttc agcttgcgtg ttatcgattt gtttttggaa
     4801 tccagaagaa agggacagaa cgatcgcgat tccgatgatc ccaatactgg aagcaaagga
     4861 ggtcaagaaa gtacgtcctt tttttgttcg gatattgttg aaggaaagct ttaacgctgt
     4921 ccaaaaactc atctttgttc tgcgtaagtt aaattgatca tcttttggtc gttcgatgtg
     4981 aggatgggaa tctgttaaga tcttaccatc agaaaattca atgattcgat ctgcgtattg
     5041 atgggccagt tcaggattat gtgtgaccat gatcactaat ttttctttcg ataactcttg
     5101 gatcaattcc atgatctgga tacttgtttc tgtatctaac gctccggttg gttcatcgca
     5161 caaaaggata tctgggtcat tggctagtgc tcgagcgatg gccactcgct gcatttgtcc
     5221 accagataac tgatttggct ttttatgcat atgatttgtc aagccgactc ggtgcaatgc
     5281 atcttctgcc cgttttcgtt tttcgtcttt agaaacacca cttaatgtca tcccaagttc
     5341 aacgttttca ataatgccta gatgaccaat caagttgtaa ctttgaaaaa tgaaaccgat
     5401 agagttgttt cggtaggcat cccagtcact gtccttaaag tctttagtag actttccgtt
     5461 gattaccata tcaccggaat cataattatc tagtccacca atcacattca gcatagtcgt
     5521 tttaccagaa ccactaggac ctaaaatcgc tacgaattct ttttgtctga aagcgacaga
     5581 cacaccatcc aatgctttgg tggtggtttc gccgacttta taatattttt ttatatcttt
     5641 taattgaagc ataatcaccc ttctcccgta ttgaaatatt cggtatacta ataagtatat
     5701 cagaacaatg atcgtagtat acgaaataaa tgttaactga agatgaacta caagaaaaaa
     5761 tctagaaatt tttcaaaaaa cagagggaga tcaatgaatc gtattttagt agtagaagat
     5821 gatccgcaat tacagttgct ttaccgttct gtgttggaac gagcgggctt ttcagtcatc
     5881 accgcattga atggagagga tgcgctgaaa aaactagaag cgacacgtat agaattaatc
     5941 attacagata tcatgatgcc gaaaatggat ggctatgact tgctagactc attacgtagc
     6001 accagatttg acacacctgt tctaatgatt acggcaaagg ccgattttga ggataagaaa
     6061 cgcgggttca aattaggagc agatgattat atgacaaaac ccatcgatgt caatgagatg
     6121 atcctacgtg tggaagcatt gcttcgacgt tcaaagatca attacagcag tagcttgata
     6181 atcggacaaa caaaattaga gctagatgct tatacagtgt tacaagatca gcattcgttg
     6241 attctgcctc aaaaagaatt tctgcttctg tataaattac tgtcttatcc caacaagatc
     6301 ttcactcgcc aacagttgat ggatgatatt tggggattag atacgaatac agaagaacga
     6361 acgatcgatg tacatatcaa acgtttaagg acccgttttg aaaagacgga agattttcaa
     6421 atcatcacag tacggggact aggctataag gcggttattt aaagatgaaa aaaaagctat
     6481 tttcacaatt atggatctat tttgctagta ttgtcttcat ttcgatctta gtgacactgc
     6541 tgtgttttct cggttttatt ttcttattgt ctcgacattc tatctcgcct gctgaatcgg
     6601 ctggaaaacc aacgttatac ccgttattcg tctttgcggg gttcagtatg gtcgtgggta
     6661 caggaatatc gatttttgtt gggcggcgta tcctgcatcc aatcagtgcg ctagggacaa
     6721 atatgagtct ggtagcaacg ggagattttt cgattcgaat ggatgaacag cagaaagtag
     6781 cagaagtaca gcaactgtat aaagatttca acgttatggt ccaagaactc aatagtatcg
     6841 agacactgag gaatgacttt gtgtccagtg tatcccatga attcaaaaca ccactagcaa
     6901 caatccaagg atatgttcaa ttgttacaag ccccaaatct ctctgacgaa gaacgccaaa
     6961 tatttttata tcggattatc gaaagtatca cgcagctttc acaattaaca gagaacatct
     7021 taaaattgaa caaactggaa aaccagcgta tccaactgga aaagaaagag tatcgattag
     7081 atgaacaaat cagagaagta atcgtttttt ttcagcctaa atgggagaaa gaacagctag
     7141 aattggatat cgaacttgct gcagtaaatt acacaggcaa tgaagagttt ttgtaccaag
     7201 tgtggctgaa tatcatggat aacgccataa agtacaatca aatcaatgga cagattcata
     7261 tcaaactatt tgagacagcc acagagattg tactggaagt gaccgattca ggtgtaggaa
     7321 tgaatgaaga gaccagagat cgaatgtttg aaaaatttta tcaaggagat acaagtcgtc
     7381 aaatttctgg gaatgggcta ggactttcac tggttaaaaa aattctagaa ctgcatgatg
     7441 gacgtattga ttatagcagt atcgaaggag taggaacgac agcaatgata cggttaagta
     7501 aacaatagga aaaagaataa aacccctaaa aattctattg aaggttttta ggggttttta
     7561 gtcgtttttt tgtgattagg gaaatgttct tacggttctt ttggcataaa aatcgctgct
     7621 ttcttattga actacgagaa atgttagagg tgtcattgaa acaaggacat gatgcaagaa
     7681 agagagcgaa agtcatgcgt cgctctcttt ttttgggtgg atagatggac agaaaatgta
     7741 gggcttagtt ttcttcttga tttaaatact cccaaaatgt atctgaagcc aaagaatgct
     7801 caccgacttt acgagtaaat gcaagataac tctctgttgt tggagaaata gggagaatca
     7861 cagtattttc ttttaataga ttcttgactg ttttttccat aacaatagca atccccatgt
     7921 catttgcaat catattgatg atcaagtcga ttcttgctcc tttataagag atttttggtt
     7981 caaatccagc ttcgcgacac agatctagta caggttgata caagttcgta gtggggccaa
     8041 ggagcaaaaa gttctccttt ttcagttcgc ttagatccaa tttcttttta ctagctaaag
     8101 gatgattttt aggtagaact gctacaaaat cgtctgtttc agtaggcaac cactcaatcg
     8161 aacttggtgg ggcctggaaa cttctagcaa agtggatcgt gtcactatgt tcattggcgt
     8221 cattgtaagt taattcgaca ctttccactt ctttcaactg gactgtaaat tcaggatggc
     8281 gtttcaaaaa ctcggtgatt ttggaaaaac ctttataatt gatcattgtc ggtatcgtta
     8341 agatatttaa tgaaagattg ttgtctttcg tataggtttc aagcacttct tgcatagcat
     8401 ggtattgttc tacgacattt tttacatatg ggagcaataa gcttccagct tcggtcaatt
     8461 cgatttttct atgggcgcgt tcgaataatt tgacgtctaa ttctgtttct aacgaaagaa
     8521 tctgtttaga aatatttcct tgggtggtga attgtcgatc agcagttttg gtgaaattca
     8581 ttgtttcggc taaatcaaga aaaacttcga atttatggat atccatcata ttcctcctac
     8641 ttattccaat aaagaatact ttaacacaaa aaatgaattg gtgtaatctt ctctgtctgt
     8701 tacaatcttt cttgcagcga aagcaataat tacagaaatt tttctgattt ttttcgcttc
     8761 gtagaaaaag agaggagttt tttgatgcca actggagttt tgatcaatgt aggttccgtt
     8821 ttgtttggcg gactaattgg cggattagta ggaaataaat taagtgaaca ttttaaagca
     8881 cagctgacga tggtttttgg tgtgtgttcg atggggatgg gaatctattc cattgcgccg
     8941 atgaaaaata tgccggctgt tatttttgct ttagtgatag gaacagcgat tggtttgatt
     9001 gttcatttag gtaatgggat aaataagggg gctgcgctga tgcaagtacc tatttctaaa
     9061 atcttcccta gtgaaaagct tggtatgacc cacgatgaat ttatcagcac attagtgacg
     9121 gtcattgttc tattttgtgc aagtggtacg gggatctatg gaagtttaga ttctgggatg
     9181 acaggagaca gtacgatttt gatttctaaa tctgtccttg attttttcac agcagctatt
     9241 tttgcgtgta atttagggta tgtcgtttct gtgatagcaa ttccccaatt cattattttt
     9301 tatattttgt tcttattggc aaaatttatt tatccattga ctacaccaga catgatctta
     9361 gattttaaag cttgcggcgg attcttgatg gtcgctaccg gttttagaat gatcaacgtg
     9421 aaaatgttcc cagtagctga catgatacct gcaatgatcg ttatcatgcc actgagctgg
     9481 atgtggacaa attggattat gccactgtta taataaaaat caaagagaag ccattttttg
     9541 ctgactaaaa aggatcaaat cttttagtca gtttttttgt atttgacaaa ctgattttga
     9601 aactatatta tatgttgtat aatatagatt gaaataaaat attacacttg agaatagaag
     9661 gtgacgtaat gcacatccag attccaactg ttcttcttga tggtactgtg ctagcagcat
     9721 taagcaagga agatatgtat gggtatgcct taacaaaaat cgtacaagaa agtctctctg
     9781 tcagtgaatc tacactatat ccggttttga gaagattgaa aaaagaagga tatcttgaga
     9841 cctatgatga accttttgaa ggcagaaacc gtcgatatta ctcgttgaca ccaatgggaa
     9901 gtcgtcattt agaagaaatt attgatgaat ggattgtttt tcgagattct ttagaccggt
     9961 tattggagga gagcacaaat gaatgaatat ttgaatgaag ttgtggatca tcttcaagaa
    10021 attcctgaag acgatcgttt tgatttgatc cagtattatg aggaatattt tttagattct
    10081 ggaaaaacat tagatcagat cattgatgaa tatggtacac caaagcagtt tgccttaaaa
    10141 ttaaaaatca gttacttttc agaagaagaa caagcagaaa acgagttagt gaaaaatcca
    10201 aaaagacaaa ttcgcctgat ttggttaatc attatcggtt tatttgcttc tcctgtcctc
    10261 attccactag cactggggtt tatattggtt attgggggac tattggttgc tttactagcg
    10321 ctcattttta gtatctatgt tttatgtatc agtatcttag gtgttggcct attcatgatc
    10381 atctcaggaa tcagtgtgtt tggacaatcg attctgagtg gattattctt tatcggtgtc
    10441 ggtctgttcg ccacaggaag cgcagtattt atttcgccac tgttgctgaa aggaacaaaa
    10501 gggctttttt atgtcatgat gaactttgtt aaatggatgg gacgacgttt tattacaaaa
    10561 agagatctgc attcagcggg cgtttgaagg aggacacata aattgagatt gaaacatttt
    10621 ttgattaccg gtattggtct gatgcttact ggtggcctcc tagcaggtgc aacttataca
    10681 ttaggcgctc aaaagagtct gacttgggat aatggtcctc ggttgatcga tatggttact
    10741 gagacaaaaa aaatcaagga tgcaaacaaa atcgtcatcc atgcagaaaa tcaaacagtt
    10801 tcggtcatac gcggattgga ttttgagata aaaacgactt atgataagac agaaaagcca
    10861 gaaataaagg agaaaaatgg cgtgttgact gtgaatgcag tcaaaaaatc aacaggtgca
    10921 atcattagtt tagaagacac aagtgcacaa atcactattg ttgtccctgc agatcatgaa
    10981 ttatctgaat tgacaatcga agggaataat agtgcaattg atatcaacga agtatcaatc
    11041 aaaaaagtga atactaagac acatgatggc tgggtatcgt tcaatgaatt tacaggagga
    11101 acaataactg cgaaagatga attaggctca cttcgattag atgaggccaa tgtggataac
    11161 ttgatcgtca caggtgaaaa cagcaacttc catctagaag acagtttgat cagcaaagaa
    11221 ggaaaagtca tcctaaacgg tggcaattta tctttgcatg agatggaaaa cagtggctat
    11281 actatccaaa cgtctggtga atcttatatt gaaaaagact accagccaat aacaaatact
    11341 ttgttagaac aaggacagaa aaagctaaag atcactgcta aagacacatc gatcaatatt
    11401 acgacggatc aagaggacaa tgaaacagat tgatcctgta ctcaactgga tattttaaga
    11461 aaaaacagca aagatccttc caatctttgc tgtttttcat cgcttaacgc acataactat
    11521 agtaaaataa gaaagaaaat atctaaaaag aaaacagggt gaaagagtat atgaaggtag
    11581 aagaaggtca aagagaaaag ttaaaaacag aaatggaaag attacatact tatatcactc
    11641 aactaagcca aaccttctat gatccagaca aagaaaaagt gatggttaat tacccgaaca
    11701 attcagaagg tcgccaatta gagcaagtgt accatgaagt tttcaaacat ttattgacgg
    11761 tgaagaaaga gctggattat tattctttac cgattatcga tacaggaata ttgaagtatg
    11821 atgggaagaa ggaacgattt atctttaaat ctgttcggga agatttgcct ctttcagcag
    11881 ggatggatct agaggtcttg gtagaagact attttacaga agaaaagcac tgggttcgaa
    11941 caaaattgga ttatttaccg caggcagccg gagggacaca ggcaagcggt tggtacatca
    12001 ctgaagacaa agagttagaa ctagaaggtg taatggcgcg tattcgaaaa aaaaattaaa
    12061 aaagtaacat tagatattta ttgaaggata aaggagcgca ggaagtagca tgagagaaaa
    12121 aagagcattt tatatcaatg gttatgcagg gattgtcgga ttggtcgtat tagctttagt
    12181 tggattgttt ttcttctatt taggtatgtg gcaagcgaaa gtttgggcac tttttttaag
    12241 tatttttctt tggttgatca cgttattgtt gttaagttcg gcaactgtag tcagtcctaa
    12301 tcaagcaaaa gtaattttgt tttttggaca atatttaggt acgattcgag aaaatggctt
    12361 tttccttacg atacctttgg cacaaaaaat gactgtttcg ttgaaagttc ggaatttcaa
    12421 tagttctgtt ttaaaagtca atgatttgga tggaaacccc atcgaaatct cagcagtagt
    12481 agtctttaaa gtcgttgata cagcaaaggc attattcgat gtagcgtatt atcaagattt
    12541 tgtagaaatc caaagtgaga cagccattcg gcatatcgct agccaatacc cgtatgatac
    12601 attcaatgag aatgatttga cgcttcgagg aaacacgacg gcagtgtctg acgagttgca
    12661 aaaagagctg caagagcgtt tagcagtagc aggtgtcgaa gtaatcgaaa cacgcttgaa
    12721 ccatttagcc tatgcaacag aaatcgctag tgccatgctc caacgtcaac aagcaaaagc
    12781 cattttgtct gctcgacaaa ccattgttga aggagctgtc acaatcactc aaatggcctt
    12841 agagcaaatc gaagatggac aagaaatcaa tttcaccgat gatcggaaag tccaattgat
    12901 caataattta cttgtctcaa tcattacaga caaaggaacg caacctgtta tcaatacagg
    12961 cgacatgaaa gaccgtaagt gacatacttt ttgtaagata ggaagaagga gtttaggacg
    13021 gattattgtc ttgaactctt tttttagttg ttttaaagaa aatgacaaaa ttgaaagaag
    13081 aatgatagtt gattcgaagg cagaattgtt cgcttttttc tataatcagt atataaagta
    13141 agagagaagg ggaaaaagtg aaaaaacttc tgatcggtct acttgttttc atcgttgtgg
    13201 tacttgcagg ggtgaaaatc gcagattacg tagttatggg tggagaagaa tactatgtac
    13261 aaattacgac agatggtaca aaaacagttt caaaatcaga ctctggacaa gaaacagtca
    13321 actatagata tatcctgcct ggctatacga aagagggtaa ggaaaaagaa ctggacttca
    13381 agggacaatt gactcgaccg cttcgtaaag gagcatattt aaaaatcacc tggaacaaaa
    13441 ataaaggtgt cacttcgtat gaagaagtcg aaaaaaaaga cgtgccgaaa acagcactgg
    13501 aaaaactaat taaagggtag ggaaagaaat gaacaaaata gtcgaagtac agaatgtaac
    13561 aaaagtatac ggaaaaaaca atgagaaaaa gacgcaagca ttaagcggca tcagcttcga
    13621 tgtagaaaaa ggcgaattta ttgggatcat gggcgcttct ggttctggta agtcgacact
    13681 tttgaatatt ttatccacat tagataaacc aacagatggg catatccgaa tcaatcaaaa
    13741 agatgtgacc acattaaaag gaaatcaact agcagatttt cgtgcgaatg agattggctt
    13801 tattttccaa gatttcaatt tattagaaag cttgacagca caagaaaata tcgctgttcc
    13861 tctttcttta caaggcgtca gaccaaaaga aatcaagcaa cgtgtccaaa aaattgcaga
    13921 acgtttatcc atctctcata ttttggaaag ctatccttct gagatttccg gtggacaaaa
    13981 acaacgggtc gccgccgcac gtgcattgat cacacaacca acgattctat taggggatga
    14041 gcctacaggt gcgctggatt ccaaaagtgc ccgggatctt ttagatacga tggacgaatt
    14101 gaatacaaaa gatcatgtat cgattcttct tgtgacgcat gatccgtttt ctgcaagtta
    14161 ttgccaacgt attttattca tcaaggatgt cggtatccat caagaagtga aacgtggaga
    14221 tcaaagccgt gattccttct atcgagaaat actatctatt cttggaaact tggaacagta
    14281 gggggagatt gactatgctt tggaaattat cacttactgg gatcaagagc cgtttaagag
    14341 actacttggt gttattctca ggattagtta tggcatctgc tattttttac atgtttgaat
    14401 caatggcaag caacgaagct tttttgaaaa gtaatacgat cgttgcggct acagttatca
    14461 ttttccgatt cggttctgtt ttactaggga tcatcacatt cgtttatatt ttatatgcaa
    14521 attccttttt gatgacgatg cggcaaaaag actatgcaat gtttatgatg ctgggagcaa
    14581 aaggcagaaa aatcgcacag atgatttttt ctgaaacatt cttagtaggg atcgcagcta
    14641 ctttagtggg ttcagctgtc ggtgtaggat tgacaagtat cgtccaccga ttattagtgg
    14701 atcagttaga tctcaatatt tcgcatttta caccttttag tattcacggg ttgttgatca
    14761 ctgttttgtt ttttgctgtt ttgtttttat tagctgctgt tgtcaatgct ttttcgattg
    14821 tcaaaaaacc gattttagca ttgattcgtg cggatcagac accaacaaga atcaagcagc
    14881 ataaattctt gtttttcctt gaagtagtac tgggaattgc ttttctaggc tgcggttact
    14941 atatgatgaa acacgtgggg acctatcaag tctttggtat aggggtagcc ttagttacga
    15001 tcgtattagg aacttatttc attttccatt ctgtgatcat tttcttttta tcattactaa
    15061 agaaatcaga acagatttca ttgaaaaaat tgaacaattt cacattgtca cagttaaact
    15121 ttcgtattcg tgaatataca caaatgctct caatggtcgc tatgctgttt gccttggcac
    15181 tgggcgcttt gacggtagga ttaggtttta aaaatgaaat tgcagacttg acagaaaaca
    15241 attctggata tgatctagtg atgaataatc ctcgggcaga agatcagcaa aaaatcaaag
    15301 aactttcacc aacactttct gcttcttata ctcaaaaaga agatgcggga acgatttact
    15361 ataatgcaag tgaatttgcg gctcaaccat tgatcatgat aaaacatgaa gatggaacac
    15421 cgccgaagat cacgaaagaa aaagttcctg ctaaaaaaat gaatgaactg actgtacaag
    15481 aggaacttcg aagctatgaa ttgccggaac agcaagaaaa agaaatcaag gtagtcagtc
    15541 aatcagaatt tgatcaactg aacttgccac aatccacttt acagctagtt caggtaaaag
    15601 acttccaagc aaacctgaaa ccaatcgaaa cacttgttgc tcaaaataaa atgaacaatc
    15661 catctttgca aaaagtagag tacagcaacc agaaatatga aatgtatgaa gtgtataatg
    15721 gtctattctc tggttttgaa tttatgggat tcttcctagg tttagctttc ttgaccatgc
    15781 ttgctagctg tttgatgttc aagatcttgt caggaagcaa gagtgacatt gctcgttatg
    15841 aaatgcttga aaagatagga acacgacgcg ggttgcttaa acaatcgatc cgtagagaag
    15901 taggcgtgtt attcttagca ccaggaatct taggtgtagt ccatgtctta ttcggattac
    15961 aaatgtttgg tctattcatg caaaatccat acaaagatat ttgggttccg tttacgatat
    16021 tcttcgtatt gtatttcatc tactatgtac tgacgacatg gttatatact ggaatcgtat
    16081 tgaaaaagag attgtg
//
LOCUS       DACEMO010000048        15916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEMO010000048 DACEMO010000000
VERSION     DACEMO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.049"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            59..406
                     /locus_tag="IW728_001162"
     CDS             59..406
                     /locus_tag="IW728_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024536.1"
                     /translation="MDHLYIKYIHQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFS
                     YAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYIIVLIF
                     LSFIVWMFYLVKK"
     gene            501..1385
                     /locus_tag="IW728_001163"
     CDS             501..1385
                     /locus_tag="IW728_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1024537.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1609..4776
                     /locus_tag="IW728_001164"
     CDS             1609..4776
                     /locus_tag="IW728_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024538.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4796..5341
                     /locus_tag="IW728_001165"
     CDS             4796..5341
                     /locus_tag="IW728_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ1024539.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5369..5824
                     /locus_tag="IW728_001166"
     CDS             5369..5824
                     /locus_tag="IW728_001166"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ1024540.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5821..6579
                     /locus_tag="IW728_001167"
     CDS             5821..6579
                     /locus_tag="IW728_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1024541.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6665..7405)
                     /locus_tag="IW728_001168"
     CDS             complement(6665..7405)
                     /locus_tag="IW728_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1024542.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7466..8719
                     /locus_tag="IW728_001169"
     CDS             7466..8719
                     /locus_tag="IW728_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1024543.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9041..9268
                     /locus_tag="IW728_001170"
     CDS             9041..9268
                     /locus_tag="IW728_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1024544.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9391..9717
                     /locus_tag="IW728_001171"
     CDS             9391..9717
                     /locus_tag="IW728_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1024545.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELVDVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9785..10498
                     /locus_tag="IW728_001172"
     CDS             9785..10498
                     /locus_tag="IW728_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1024546.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10808..11659
                     /locus_tag="IW728_001173"
     CDS             10808..11659
                     /locus_tag="IW728_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1024547.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11780..13645
                     /locus_tag="IW728_001174"
     CDS             11780..13645
                     /locus_tag="IW728_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1024548.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13693..15102
                     /locus_tag="IW728_001175"
     CDS             13693..15102
                     /locus_tag="IW728_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1024549.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15210..15911
                     /locus_tag="IW728_001176"
     CDS             15210..15911
                     /locus_tag="IW728_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1024550.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
BASE COUNT         5305 a         2682 c         3428 g         4501 t
ORIGIN      
        1 agaagttatt cacgtatacg gatttgtttt tctcacaaat aaaaaggcag gaagtacaat
       61 ggatcatttg tatataaaat atattcatca attttccgtt ttggtgttag gcacactaat
      121 tatagcgggg attgtcgcat ttattattca aggccgtgac atattaacag tacatcaaga
      181 gtttttcagt tatgcatttg gtattcctct gatcgctata aggtctgata acaaaaagtg
      241 gaagaaacgc tattacattc tcttgctatt tttgtgtttt tatctgccac ctaagatttt
      301 tactttatta ggtatacaat gcctatcatt ttcttttatt tcctatatca ttgttttaat
      361 tttcctcagt ttcatagtat ggatgtttta tcttgtcaaa aaataaatga atgtttcatg
      421 aacagtactt catcacagcc taattttttt ctttatgcta tcgttatatt aaagaaatca
      481 atcagaaggg aaagaaagtg atgaaaaaag ttttattagt ggtaaacccc agttcaggcg
      541 gtgaacaagc aaaggaattt gagcaattag cgatagcaaa attggaatcg gtgtttgatg
      601 aagtggtcgt cttgcataca aaaaaagcag gggatgcaaa aaactttact cgcgaagcgg
      661 ctacggaagg gtatcatagt gtgtttgtca tgggcggaga tggaacagtc aacgaaggaa
      721 tcagtggaat cgctgaacaa gaacatcggc caaatttcgg atttttccca ttgggtacgg
      781 tgaatgactt ggctcgagca ttagggatac cgctagaacc tgaggaagcg atcaatcatt
      841 tttcgattga atcagtcaag ccattggata ttggaaaaat aaatgacgac tattttatga
      901 atgtcgtcgc aattggctct atccctgaat caatcaatga tgtagatgcg gagaagaaga
      961 cgaaatttgg aaaactagct tatttcatgt caggaatcaa acagttagct tccacacaat
     1021 cctactcgtt tcacgtagaa gttgatggaa agaaggaaga aattgagagc agtaccttgc
     1081 tgataggact aactaactca gtcggcggtt ttgaaacatt acttccaaat gcaaaagtgg
     1141 atgatggtaa gctccatttg gtttatttaa aagattcttc tttgatcgat acgcttaaaa
     1201 cattgccaga cctattaaaa ggagtagatg aatctacgaa aaatctagcc taccttactt
     1261 gtgagcaatt agttgtgtca ttagctgatg aggaagaatt gacgacgaat gttgatggag
     1321 atgaagggga taaattacct gtgacaatca aagtattgcc ctcacattta caggtttatt
     1381 gttaaaatgg aggttgtgac cgaggagttg gcgggagaac accgcttgtt cgggataagg
     1441 gactgtgaca agacttttgt cacagccttt tttttgtaca aaaaagaaat ttacacctaa
     1501 tagaaaaatt tttccataaa aatacatatt ttaaatatga aagtacaatt ggaaatagat
     1561 taaaacgata aaataaaatt gtcaaaacgt cagagaggat ggtggagaat gaatcaatgt
     1621 gtaaaaaagc tgattgcgtc gattttgttt gtcttccctc tcttgatggt gacatccgtt
     1681 tttgccgatg tagagaaaac aaacgaatca acgcaatcag agaatcaaag ttttgacaag
     1741 actacttttt cagaagaatc gattacttct gaaactacag aatctcaaag tgaggcttct
     1801 actaataaaa ggcaagaaga aaataagaca agtgagacct cagaagtagc tgaacaaacg
     1861 tctgatagca gtgaagcggc actggcaaga gctgtttcag cggatgggta cagcgaagct
     1921 gccacaacag aagaactggc gcagcttctc gcagacgaat cagtaactaa gatccgactg
     1981 attcagcctt tgactttaga cagggaatta gagatcaaac gagatatcgt tattgatttt
     2041 ggtggattcg ctcacaattt cggcacacac catatttata tcaatgagat acctcagtcg
     2101 atcgagttcc agaattttaa aggaacagct gcacatccag gtggattgac accaaatatc
     2161 gatggaaatg ccattatcat tgcttatatg accgattttt ggggaagcag atatcatttt
     2221 acgggagaaa taaaatttac cgggacactt gatttgtatg atgggtccaa attaggactg
     2281 atttacgctc ctcgtgcaac tgtcacatta gatggtgttt ccggtgtgtt ggatgtccaa
     2341 ccagtgaagg aaggactgaa tgccgctccg ggtaaagcct atttttgtag aagctatcaa
     2401 ttgaatgtga tcaatggtag tcaactttat ggaccgtatc tagggaaatt ctacggtttt
     2461 ctagattcag gagatgagac tggtagtgga aaaacagcgc caggaatcca tatcttaagc
     2521 ggttcaaagg tctctcttga ttatgatcgg gcagatggcg ttagcgatga aggagaggca
     2581 gtcgatacgc tacctggtaa tgtcatgttt gaagtcagtg gttcgggaag cgaattttcg
     2641 gtaaatacaa agatcaatat taccaatgac aacaatcgcg gaatcatcca aatgagaggt
     2701 tcaggatcga aagtcactgt atcgaatggt gggaaaatca ccatcaatac agcaactacg
     2761 ggcggatttc gcttgcaagg agattctagt caaatccatg tatcaagtgg tggacaaatc
     2821 tcggtcaaaa tggctggaga tgacgatcaa cccgggaaca atgggatgcg ttttgtagga
     2881 aaaggccttt ctttgatcgt agaaggagcc ggatcagtta tcgacatcga taagcagagt
     2941 gggcgttcca gcgcagttcg ttttgagaat ggtacgcaaa agctgacagt aaccaatgga
     3001 ggctcattga gggtaagaaa tacaggtgat ggcaaatctt acgtgaacag actcaatcaa
     3061 ggaaatcaag cgatccaatt ttatgacgct tctggttttc tagaaagtcc aggttcagct
     3121 gattttacag tcactggaga aaaatcaaat atcgatatac gcgcagattc cggagcaact
     3181 gtagatacaa ctggagatat tgatttaaat tttacggcag gtgaaaaaac ttatttagtc
     3241 atggtcggga aaacagggga agataagacc ggcatcatca gtggggatgt gttgaatgtg
     3301 gacttagaat ctcctaccta ttttgatttc caaaacaaac ggattggaag cgagaccaat
     3361 ggcataggcg gctgggtatt ccaaggaaat ggagattctt ctttaaagat cgcccattca
     3421 gaaatcgctt tatggcgaaa aacaggcgca acattcgata actttgaaaa agacccggat
     3481 tatttttctg atatgacaga cttatcgatc aatgggaccg acttaggcaa tttcgtatca
     3541 agccctagtg caggtttaac aaatgaattt gctcagacag gtgccggaat gaaaaattat
     3601 aatcgaatca gtgccaataa tcagctcccg atgattgaat ccttaagagt gccaacgaat
     3661 gcagataaaa aaatctatgg gcatgtcgtt gttcccgaag gtgtcgaggc aaagccaaga
     3721 gatgcttgga caggcgaagt agaagtgact ttacgtatta cttacgcaga tacgacgaaa
     3781 tcatctgtag aaattactgc tctcacgcaa ggacaaacag atgaaaatga aggggcagga
     3841 tataacccat ggggagaagg cgaacaaggc ggcttgttcg aagttcaagt acccaatgat
     3901 gagagattaa aaacggagga tcaagtgcaa gttgttgctg ctaaaaaagt aagaggtggc
     3961 caagaagtga cagaactgga ctctgccaaa acgacagtcg atgtgattcc tcctgaacct
     4021 gctaatttaa gtaccacatt gatcaatgca gcttctcgca cgatcacagg tacaggtgaa
     4081 gcaggagcct tggtttcatt gaaaaaaaat caagctgttt tgtcagaaac aactattgat
     4141 gatacagggg aatttgttct ttctatccca gaaaatcaac tttttgcagg agatgtatta
     4201 gaaattgtat taaacgacca agcaggagaa gctactgcta agggtgtgca gaacaagcca
     4261 agtaccaacg atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat
     4321 gccccgcctt ttgcacctgc tacgaagatc gtagtagaag gtggtctctc atttcttgcc
     4381 ccttccaaac tcgattttgg tcagatcaaa gcaactggtt tgacacaaga agcatttggc
     4441 caaatggata tacaggaaaa attatggatt tatgatcagc gagaaacgaa aaatccgtgg
     4501 atcttaacga taaaattatc tcagcctttc acaaatgatg caggattttc tatgcaggat
     4561 gtcctatatt tcaagaaagg agaggagtat caattagtga atgaagaaga actggaaatt
     4621 gtcagaggac aaaattcaac gaatgtgcaa gcagattatt cagaatattt aaataacggt
     4681 cgggcgttta aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag
     4741 ataacatgga cattagcaga tgcgccgaca gaataaagat caaggaggat gattgatgaa
     4801 tcgaatatgg aataaaagtc atcgaaacaa ctgctcttta cttatgatcc ttctccttgg
     4861 attcatcctt tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga
     4921 attaccggct aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg
     4981 gaaaacacag gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga
     5041 gacattgatc cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa
     5101 aagtaaattt gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga
     5161 aataactgtt cctcctcaaa gtgcggtcac tgagacactt tcactgaaag cgccagaaaa
     5221 agagctggct ggtgtggtcg caggagggat cacgtttcaa gaaaaaaaag agaacgtttc
     5281 taacaaaagt acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgatttg
     5341 aaatagcgaa aaagagtctt ccccagattt gaaattaaaa aaagcttatg cggatcaaag
     5401 cgatggaaga aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg
     5461 cttgactaca tcggttgaaa tctacaaaaa agaaacaaaa gagccttacc tttcaataaa
     5521 aaaagaaaaa atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg
     5581 aaaaaaatta gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg
     5641 ctggaatctc cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa
     5701 ggatattgat ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg
     5761 agtcattctt attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa
     5821 atgaaaaaaa caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa
     5881 agtgtttttg ctgagaataa gagctcagcc actacagaag gaacgatttc tgtgaccaaa
     5941 ccagatgctg caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatcct
     6001 gatactcctt ttacacctga cgaaccaaat cagggaacat ccggattact gacgattgac
     6061 caagctccag atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct
     6121 gcattacaaa gcggaacgga cagtactgga gcacaaacac aagtacagaa tttcgtacaa
     6181 gtaacggata aaagcggaaa ttatgccggt tggaaattat cagtcaaacg aacagaattt
     6241 attggtacaa catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc
     6301 gtgattcgaa atagcgcaac taatagcagt ggaatgccat ctaccgtttc tggaactggc
     6361 gctgaaggaa tcgaaatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat
     6421 gaagggattg gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca
     6481 gtggaattat ttgttcctgt atccaattat gctgtagaca actacgtttc aacattcaca
     6541 tggtcgttaa cagatgctcc tacagaagag gaagcgtaaa atcatatcaa cgatttcagg
     6601 gagtggagcc gattcaaggc ccactccctg aaatctgcta tgcttacgtc tcagctctta
     6661 ttcatcaact cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga
     6721 tctttcgacc aacgagtaag gagtcaccgt tgaaaaattc taacacataa tgagcagaat
     6781 caattttctg caattgcgat aagttgatac agtaagattt taaaagtaaa aagtaagcat
     6841 acttttctaa ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag
     6901 ttgttttgac cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc
     6961 gaggatacag gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga
     7021 tctgctgttc tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc
     7081 tgtcttttct gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat
     7141 tcgtataagc ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg
     7201 cgacatcaat acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat
     7261 tatcaatagt gcccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga
     7321 tattctcgat atgatgattg accaattctc tgatgatcca ttcgctgtga aactggttat
     7381 cttctatgat aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt
     7441 tgaaacaaaa caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac
     7501 tttgatttta gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag
     7561 ctttggttgt atagctttga tggtggtgac acttttactt cctatcccta aagtgagctt
     7621 tccttaccta gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt
     7681 tatcagctgt ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt
     7741 ttttgatctg atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact
     7801 acttatcgca tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct
     7861 caacttgctt tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag
     7921 ctggggcgtg atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct
     7981 tctatctctt ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat
     8041 cagtttccaa gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga
     8101 agaatattac catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt
     8161 aaaaattact cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tattagaaga
     8221 tcattctgca gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc
     8281 agctgtcagg ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat
     8341 ccaagtagtt attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg
     8401 ctgtttatct atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat
     8461 tacgatcaat ctgctggaag aaaaagaggg atcacgtttt agtatagcga acaaggcaaa
     8521 tccaacgaat ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg
     8581 cgggttaggt ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa
     8641 aatcgaattt tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa
     8701 gagcaaatca gcctactgac ttactgtcaa aggataagtt tgaggaaagt ctacacggag
     8761 atagagacaa caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct
     8821 tctgtttctt ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag
     8881 tacgaaagta tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg
     8941 tcaaaatgct aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta
     9001 ggcgtggaga agcaagcaaa tccctacgat cacaaaaaaa atgctgaccc agcagttaag
     9061 agagttagaa caagatgaaa gtatccatcg aaaagcctat aaccaagtcc cgcctaaagt
     9121 agagtactcg ctcaccgaag aaggaaaatc attaagagag attctagtag cgttgtctgt
     9181 ttgggaagaa gaacgtatcg aaaaacaaaa agcctcaagg gaagaagaca cgctactgca
     9241 tacacatggt ggctacctaa attattgatt ttctatgaca tttattttaa ataaaagaaa
     9301 acaggagttt ttttaaaagc atcatctatt ttttatgtaa aaaaatggta tgattaatca
     9361 agtaagaagt gattggaaga aggcaagcaa atgaatagta tggaaaaagt caaccagttt
     9421 agagatgaac gtaattggcg acaatttcat aacgaaaaag atttggctat ctctatttct
     9481 ttggaagcat ctgaattgtt agaacttttt caatggaaaa cgccggaaga agtaaaagaa
     9541 acacaattag aacgaatcaa agaagaactg gtagatgtat tgatctattc atacatgatg
     9601 gcagacaacc ttcacctgga tctagatgag atcattgaag aaaaattgat caaaaataat
     9661 ttgaaatatc ctgtagaaaa aagcgcaggt gtacgaaaaa aatacacaga gttgtagaaa
     9721 tgtgtcattc ggctgttgga aagagcaaaa gaaaaacagt aaaatgagtc tggagggaga
     9781 gaaaatgaaa aaaaaaggaa aagcaaaaag atggctagtc aatatcctct tgttcctctt
     9841 gttattagta ggactagctt tgattttcaa tgaacagatc aaggattatt ttgtcagaga
     9901 gacaggagat aaatatgcta tagcgaatgt aacaaaagaa gatctgaaaa agaataacga
     9961 taaagatgtc agttttgact ttgatgcggt agaaccaatg acgacagaag gggtcatgag
    10021 gtcgcaaatg agaggaacag atctaccagt gattgctagt atcgcagtcc cttcagtttc
    10081 aatcaattta ccggttttca aaggcttgga taatacgtca ctactttacg gagcaggaac
    10141 attatctcca gatcaagaaa tgggaaaagg aaattatgcc ttagcaagcc atcgtgcaac
    10201 gaaccctgaa ttgctgttta caccgctaga aaatttagag atgggtgcca agatctattt
    10261 gacagattta gaaaatgtgt atacatacaa aacatttttt aaagaaaaag ttgctcctac
    10321 agatacccaa ttactgaatg aagtcgaggg aaaagaaatt gtcacactaa ttacttgtgg
    10381 cgacatggac gccgtcacgc gactagttgt acaaggagaa ctggaaagtg ttacatcgat
    10441 aaaagatgcg acagatgata tgagatctgc tttcaatcta gagacaaaaa cattttagga
    10501 tactgggaca taagtcgtta cagattaaaa acagccaaag gatatgaatc gataaggaaa
    10561 aaccaatttg ctgaaaaaaa gagaaacgca aagaggtgca tctctgattt ggttgttttc
    10621 tagatagact tgtgttccag tttttttatt ttttatatga aaaggcttgc ataaataaaa
    10681 attcgatggt atgatgattt tgaataagaa atggattgtt actgtacggc aggcaaaacc
    10741 agaattcagc taagaaacag taggcgaatt ctaggttttt ttatttttct tgagagggcg
    10801 aagcaaaatg aaaattgata aaattttgaa taacaatgtc gtcatttcga aaaatggatt
    10861 cggggaagaa gtcgtttgta tgggaaaagg actagctttt caaaaaaaga ttggggacga
    10921 gatttcgcca gaagcggtcc aaaaagaatt tgttttaaga gattcttttg ctaaaaacca
    10981 attccagcag ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt
    11041 cgaactggct gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac
    11101 cgatcatctt cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt
    11161 attatttgaa acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt
    11221 gttcgtgaaa caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt
    11281 tcattttgtg aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac
    11341 aatggtaaga gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga
    11401 atctttgaat tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa
    11461 aggggaagga tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc
    11521 aaaagcgttt cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga
    11581 aatggggatc gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa
    11641 aaaaagcatc caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa
    11701 taggttcacg aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa
    11761 aagaaagaag gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt
    11821 aggaggacaa aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt
    11881 aaaagatgaa tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt
    11941 cgtacaaagt ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca
    12001 atcgatcgtg gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa
    12061 tattttgaat cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat
    12121 ggcagccgcc ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac
    12181 agctgatagc ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt
    12241 acctgtcatg cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt
    12301 agcaattgct atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc
    12361 tatcagtttc tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat
    12421 accaatcatt ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat
    12481 tccgcaattt ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt
    12541 aacatttctt gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt
    12601 caactcgatt tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca
    12661 agtattcgtg atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga
    12721 acaatatggg tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg
    12781 ggctgcttta gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc
    12841 ttctacagtg acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc
    12901 tttgaagaaa ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg
    12961 attttccgga gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt
    13021 cattaatacg gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat
    13081 tgcttttgtt cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga
    13141 caatcaatta gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac
    13201 cttaaaaagt cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt
    13261 ttcttctggc gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc
    13321 acctgcagac ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac
    13381 gactgacgga gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa
    13441 tggctttgag atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg
    13501 ttttgacatc gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac
    13561 gaatactgat gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga
    13621 agatgttctg gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa
    13681 aatttttatg gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac
    13741 ggaaaaggac tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc
    13801 ggcagtgaag aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga
    13861 ggaatcgatc attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc
    13921 aaatgttatc gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact
    13981 cctaacgaag cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat
    14041 attgaacctg tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac
    14101 ggtggctgga aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta
    14161 gagagataca gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt
    14221 cactttccag ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat
    14281 attttccaag catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac
    14341 gaattgcgtt cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc
    14401 gatgccaatc cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc
    14461 tgcacggatg tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa
    14521 tatggagtcg atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa
    14581 tatccagtgg actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat
    14641 ccacaagcag caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag
    14701 gcaagtgaat ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta
    14761 tacaatcgtt accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat
    14821 atggaagagg atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc
    14881 gaagcgatgg ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga
    14941 tgtatcgatc ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat
    15001 gtggatctag acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat
    15061 tggtataaag aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa
    15121 aaagaacaca cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt
    15181 ttagtaatag taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag
    15241 tcgcagataa aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc
    15301 cgacagaaag tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa
    15361 tcgaactgct ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc
    15421 taagcaatcg tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga
    15481 actcccgata tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg
    15541 aagatccttt gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt
    15601 tgaacaatca gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca
    15661 gcagagaaga atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta
    15721 ttgactcatt ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc
    15781 tgaaaacaga cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg
    15841 aaatgattga atattcggag gcggtctacc atacggagct gcatccttac catattgatt
    15901 atgaaacata aataaa
//
LOCUS       DACEMO010000049        15873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEMO010000049 DACEMO010000000
VERSION     DACEMO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.050"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            111..779
                     /locus_tag="IW728_001177"
     CDS             111..779
                     /locus_tag="IW728_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1024551.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(979..1209)
                     /locus_tag="IW728_001178"
     CDS             complement(979..1209)
                     /locus_tag="IW728_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024552.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1336..2718)
                     /locus_tag="IW728_001179"
     CDS             complement(1336..2718)
                     /locus_tag="IW728_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1024553.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2874..4061)
                     /locus_tag="IW728_001180"
     CDS             complement(2874..4061)
                     /locus_tag="IW728_001180"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1024554.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4068..5099)
                     /locus_tag="IW728_001181"
     CDS             complement(4068..5099)
                     /locus_tag="IW728_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1024555.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5102..6568)
                     /locus_tag="IW728_001182"
     CDS             complement(5102..6568)
                     /locus_tag="IW728_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1024556.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPPIKKGS"
     gene            6743..7648
                     /locus_tag="IW728_001183"
     CDS             6743..7648
                     /locus_tag="IW728_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1024557.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7739..8416
                     /locus_tag="IW728_001184"
     CDS             7739..8416
                     /locus_tag="IW728_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1024558.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8697..9593
                     /locus_tag="IW728_001185"
     CDS             8697..9593
                     /locus_tag="IW728_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1024559.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9648..11018)
                     /locus_tag="IW728_001186"
     CDS             complement(9648..11018)
                     /locus_tag="IW728_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1024560.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11256..12002
                     /locus_tag="IW728_001187"
     CDS             11256..12002
                     /locus_tag="IW728_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024561.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            11999..12838
                     /locus_tag="IW728_001188"
     CDS             11999..12838
                     /locus_tag="IW728_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1024562.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12839..13789
                     /locus_tag="IW728_001189"
     CDS             12839..13789
                     /locus_tag="IW728_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1024563.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            13930..14682
                     /locus_tag="IW728_001190"
     CDS             13930..14682
                     /locus_tag="IW728_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024564.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14701..15852
                     /gene="queG"
                     /locus_tag="IW728_001191"
     CDS             14701..15852
                     /gene="queG"
                     /locus_tag="IW728_001191"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1024565.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5147 a         2976 c         3199 g         4551 t
ORIGIN      
        1 tgtcacagtc ctttttttag taaaaggata catttcttcc ttgtatattc catgcgtgtt
       61 cgtgagaaag catatataat ttaattaaca gatagttagg gggagaaaaa atgatttttt
      121 caattacaat gaaactggtg ttcgggttga tcggtttatt attagtggta cgtttgcttg
      181 ggaagaaatc catgtcagaa atcacaccgt ttgatttagt ctatacgctt gttttaggtg
      241 ggatcttaga agaatcaacc tatgatgaca atgtccatgt agggcatgtc ctatttgcca
      301 ttgcgctttg ggcagtaatg atttatggta tcgaacgtat cgtccaaaaa aatgaaaagg
      361 tcaatcgttg ggtcaaaggc gagccatctg ttctaatcaa agacggcgtc atcaacatga
      421 cagagttgac aaataaccat atcgagatgg agcagttgcg agcaatctta agacagcaag
      481 aatgcttttt tcttgaaaat gccaaacatg ttatcctaga gaatgccggt caaatgagtg
      541 tattgaaaaa atcagatgaa gacaaagctt tatcgatcct gcttgtagat gaagggcaaa
      601 ttcagcataa agtgcttcag tccaatcaat tgactgaagc atggcttatg gagaacttga
      661 agaaagaagg ctatgcagat gtaaaacagc tgatttatgt tgaatggtca gaagaaaaag
      721 ggctctacat actaaataaa gaagacgtca tgcatgaatc ttaccgaata gatggataga
      781 tagacagagg ttgtgaaatc agcgccttgg cttgttactc aaaactaagc gactttttca
      841 caacctctga tatctaatat acgttgttcc agaagttgct tcttcgaaaa ataagccaaa
      901 attccacaaa aatttaagaa gcaattttcg aaaaatcctt tcttattttt tgaagttgat
      961 cacttctgtc acaacctctc atgtcacaaa ccattttttt gtttccttta actcaatata
     1021 ccgtacgtct tcccacaata tcggctgatg gtcaatcata aagtaatctt cataggcttc
     1081 accaatgaag tgcccttcga cctcatccaa gagtcttcca aattcatctt ttaaattgag
     1141 ctgaatgatt actgctttac gagataaaaa ggcttctgtc agtatactat cgatttcttg
     1201 caatgtcatt tgttgtttag gaagaggctg tttgcgtgct tcttcttcat ttttttgata
     1261 ccgttgacta gttcttccat cgcaaaagca gtcccccatt ttaatccaaa tggccgatct
     1321 cgatactcgt tatagtcaat gaattttttt gctgtccgtc tcatcttttc caccttctaa
     1381 tccactcata ccacctgcat gcccgccaac gagagaacta cgtgcgatgg cgcgtcctcc
     1441 ttgcaagaga ctatgcgcat ggatgattga ttgaaagccg tatttttttc taatcgtatc
     1501 gacaacaaaa tcaactttcg cttcatttat ctgcttttca ggatcttcaa aaaggttaag
     1561 ctgtaaacct gtcgcgtata cgaggtcgga acagttcacg cctacacgcc gaatatcctg
     1621 gtagtggtaa tgctggtgga aaatggtcaa taaagcatct gctagatctc tgctagcatt
     1681 tgtcgcttcg attttcattt gctggtgaaa cccggtcttg ccatttggat caagatagcc
     1741 taaagagtat cctacccata agctgactac ttgtgttttt gctgcagatc gtctcaagcg
     1801 agtgccgact tggtcggcca tttctttgat aacgatctcg atttcttctc gtcttgtata
     1861 atctctatct aggatttgac tatttccgat actttttgat tttggtgtat attcttgtcc
     1921 caaaaaactt cgatcgatgc cccaagcatg ggcataaagc tgagtaccga tgacccctag
     1981 cttgcttttc aagtgataat aatccgcgtg agctaaatca taaacagaat gtatacccat
     2041 ttgtttcaga cgttttgctg ttcgtttgcc gattccccaa aagtcagtga tatctgagat
     2101 tttccataac ttttctggta catcttcata acgccattca gcaaacattt gctttccgtc
     2161 ttttgagcca ttgtctaagg ctagtttagc aagtagcggg ttatcgccaa ttcctatagt
     2221 cgtgtagatt ccggtctgct caaacacatc tttatggatc agtttagcca tgtcgtacgc
     2281 atttgtttta ccaaagagtg ccatgctatt tgttacatca aggaagcttt catcgacgga
     2341 atagacatga tggttcgctt cgtctgcata ttttttatag atagaattga tttctctgtt
     2401 cttttccata taataggcca tccttggggc aacaatatat aaatcacttg gataggggaa
     2461 gggaagatct ctggcacggc tgatattggt gatgccgtaa gctttttttg ctgcaggact
     2521 ggaagccaag attaatccgc taccccgtgt ttctgtcgaa tcggacggat aggacatgac
     2581 tactaattta gttgtaaggg gatcaagatc cttttcaacg cactctacgg aggcgtaaaa
     2641 agatttgcag tcaatacata aaatatctct tgaaggttct ttttcataaa caaataccgg
     2701 attttgattc gcccacataa caatcacctc ttcaacacaa ttagaacaca cgttctcttt
     2761 ccgtgtcaat aaaaaacagc aattttcaaa tacgagcaaa aggctcacat ttggaaattg
     2821 ctgagaggtt atttttatct atttgttttt agctgagatc gttgattttt ttatcaaaca
     2881 tgaacagctt ctggatcgat ctttgttgtc ccatttccaa tgtgtgcaac ataaaattcc
     2941 ggagcatagc cgacaacttt catgtattca tcatagacat tgtttttgaa ggcaggtata
     3001 ttttcttctt tgacaagagc aatcgcacag ccaccaaatc cagctcctgt cattcgtgca
     3061 cctaagacac cttcttgttt ttgagcagca tctactagtg tatctaattc gatcccagtt
     3121 acttcataat catagcgaag agagtggtga gaatcattta acagttgacc aaatgcttct
     3181 aagttcccag cttctaattc agcttttgcc ttcaaagtcc gctgattttc agaaacggca
     3241 tggcgagctc tgcgtatcaa tgtatcatcg ccgatttggt caatagttga ttcaaattct
     3301 tcttcatcta agtctcctaa agaagagata ggcaaagttt tttgcaaacg tgccaaggct
     3361 tcttcgcatt cgctacggcg ttcattgtat tttgaatctg ccaattcccg ccgcttattc
     3421 gtattcatga tgacgattgc ataaccatct agtttcactg gaaccatttc gtatttcagc
     3481 gtatttgtat ctaataagat tgcatgatct agctcaccga agccgatggc aaactgatcc
     3541 atgatacctg aattgacacc gatgaattca ttttctactt ttttacctgt ttggactagt
     3601 tccaagcgat ctgtttttaa atcaaacaga tcttctaata caacacctac taataattct
     3661 aatgaagcgc tactggaaag tcctgcacca ttagggattg ttccttccac taataactca
     3721 aacccatgat cgattgagtg tccagcttct tttaatttaa ggaccattcc ttttacgtaa
     3781 tttgcccagt ttgctttttt atcatattct agctcatcta aagtaaaact aatgacaccg
     3841 atatcgtcaa agtttgtgga ataaactaat attttattat cgtctcgagg tgctgctaca
     3901 ccttgcgttc cataagtgat ggaagcgggg aagacatggc ctccattgta atctgtgtgt
     3961 tcaccaatca aattgatgcg tcctggtgag aagtattgtg tagttttttt aggtccgaat
     4021 gtttcggcaa attttgtgtt taagcttctt ttgatttcca tgatgagtta ttcctttctt
     4081 gttttgattt tgaattctgt tactgtttca aatactgatc cggcttttaa atgaatcgaa
     4141 ccaaatgctg agaagcgctc tgcacctgga gctgtttgag tctcaaatgt gatgccaccg
     4201 tgatgtgcca gttttcggtt gcgcatttct ggtgtttctg tgccgaaatt tgcagtgaaa
     4261 ataaccacac tagaagcatc tgtagcaata tctacttgga ttcttttatc ggggctagtc
     4321 aagcgagcgg cttctttacc caagccggtt tcttttaaga aaaatggatg atcgattccg
     4381 tcaacaagat ttttttgatc caaatcacta gcaaaaacgg acgctaattt tttaggtatt
     4441 tgaaaatcga aagcacttcc tgcagcattt tcttttaccc ctataggaat actatctgtt
     4501 cgcaaaggag cataagcttc gctattgagc caaagctcat gttgatcgat tgactgagaa
     4561 gcatctcccg tcaagttgaa gtaaacatga tttgtcggat tgaataaagt atcttggtca
     4621 ctggtgccgc gagaggtcac tcgccagata ttatcttttg tcaaagtata gcggatttcc
     4681 acatccattg tacctggaaa accatgttct ccgtcgggac ttgtagtagt aaaaatgaca
     4741 gaagcttcat tttcgccatt caaaatgaca taagaccatt tttttaattc aaatccagga
     4801 gcaccaccat gcagattatg tccagttttt ggatctgtgg ctaattggta agtttttcca
     4861 ttgagagaaa agcggccatt ttcgattctc cctgcggtgc gaccgataga agcaccgata
     4921 tacggatctt tttcgatata ttcttcagcg gtgtcaaatc ccaaaactaa ttctcgatca
     4981 tttgatttta aactgacgat tctagcacct aaatcagaga tagaaagtgc tagcttgttt
     5041 ttgttctcca aggttatcaa gtgataacct tggccaaaat ctttttctgt aatcttcatt
     5101 ttcaagatcc tttcttaatg ggaggaacta ctgcatcgtg aagttcatct tccattgtat
     5161 cagctggagc caatttgttt tccaatttct caacgatttg tgcatgtttt ttctctgtca
     5221 atgtaatctt ccaagcaaaa ataatagcgg agaaaatcat caaagcagca ggagcataga
     5281 atgcataagc tttgaaagtc gttacgccat gagcagtgat atcagcagca gtagcattac
     5341 cagtcatccc agaagcgata gcgacaaacc ctacgatccc gttagacaaa gcaccagcga
     5401 ttttatctag taaagggcga attgatagag taacagcttc gttacggata ccatttttcc
     5461 attgaccata ttcaacagag tctgtgatcg tcatcaaagc ggataagaaa atcaattgtt
     5521 gagggaagta gaacaatact agcccgatga taacgattgg caaacttgaa cctgcgatcg
     5581 taaagaaaaa gtaagaagaa atcatcattg cgatccctcc aaggtaaacg tagcgacgag
     5641 agatgacgcg tgtaagcatt gggaatagtg gaacagctac taaaccaagt aaggcagcaa
     5701 ctagaccaac gatccaaaat tcagttggtt ggccaagtac atatttgaag aagtagaata
     5761 atacggcagt tgttgctaca ttggcaatcg cataagcgat ataagcaagg gcaagccaca
     5821 tcaactgatc attttgagca actgctttga acacatcttt gatcccagct ttttcatttt
     5881 gctgacggat cgcagtatcg acttcttttg tgcccaaggc agtgataatc gcagtaccgc
     5941 ctgaaataac agcagcaatc acaccgaacc agaaccagcc tgaagcgcct tcaatatgtt
     6001 tatgagtaac aagataggta aagaaagtaa cgatagggac aacgactaga gtagtaccat
     6061 ttgcacctag agaagagccg aaacgagcaa acgtagcaaa tttttcacgt tcctttgttt
     6121 ccgtactcaa agctggaatc attgaccaaa aagcgatatc cttaaatgaa taaaaactat
     6181 ccaataagat gaaaacgatt gcaaataata tgataaataa agtcgtatta tgagtagcta
     6241 agccaaagaa attggtaaag attgctacca agagtacgga actaacaacg ccgccgataa
     6301 ctaaccatgg tttgaattta ccgtattttg tacgagtatt atcgatgatg ccaccaatca
     6361 aaggatcaaa aataatttca actaagcgga tacctacaac taagcttgta actgtagcaa
     6421 tcatttttgc attcgttgcc ttatctgttc ctgcgaacat ctggcttgtc acgaatacca
     6481 taaaataagt gcttaatgta acatagaatg tgtcgtgtcc aaaagcgcct aaagcgtagg
     6541 acacacgttg gatcattttg cttttcataa tctgtattcc tctcctgttc aatatataat
     6601 actaagtaaa ccggttacaa cgaataacaa agatctttta atgacttaaa cttaacaaga
     6661 aatcgcttcc tttttaatat atttctgtga cttttattta actttttgtt gcattatact
     6721 aaaggagtaa ggagggatag acgtggagca atcgattttt ttaaaaaagg aacatcaaca
     6781 agtagaaaaa gaattgtatt ttgatttttg tggtttctct aaaacgatgc cgtttcattc
     6841 ttttggtcct gctattcgca atgattacat cctgcatgtc gtgatggaag gcaaaggatc
     6901 ttatcatgtg aaaaatcaac aattccaatt gaaaaaaggt gatctgttct tgattcgtcc
     6961 aggagactcg acgttttact tatcagatgg agaagatccg tggatgtatt gctggctttc
     7021 atttggtggg agtgctgcag aagaaatcat tcggcagtcg cttttcagag aagaccaata
     7081 cacgatggtc tcttctgaca tacaaaaata tatcgacatt attttagaat gcatgaaaga
     7141 tacggaagaa accttggaaa atgaattaca gttaacagca ttaacctacc gttttttatc
     7201 agtcctttta aaagatggcg gaaaggtcag cggtggtgta caaaaggttc attcctcttt
     7261 agctgttgaa acagtcaaat acatcgaggc acattatgct gaaaaattgt ctgttgagga
     7321 aatcgcccag catttagcgg tcaatcggag ccatttgtcg cgagtattca aaaatcatat
     7381 gggtaccagt atcaaggaat atttgatcgg ggtacggatc aatcgagggg cgtttcttct
     7441 ttctctgacg gatgaatccg tggaaagtat tgcctatcaa gtagggttca atagtctagt
     7501 cgtatttagc cgaatgttca aaaagagtac cggagaaacg gctacgaact atcgtaaacg
     7561 tatgaaaaac gaaaatttta agaatattgc tttggatacc ttgaaaaaac agttggaaaa
     7621 acaaaccgca gtttcttgga caacctgatt agtctggtta aaatgacaag catcggcaaa
     7681 agaaacttgt ggcggttatg ctacactgta aagcgaagac taaaaaggag gaaaagggat
     7741 gcgtacactt atcatcatta gccatccaac gatcgaagaa tctttaaatc agcaattttt
     7801 caaagaagct tctgctggtt taccagctac ttggcatcat ttggaatcaa gttatccaga
     7861 cggaaaaata gatatagaga ctgaactaca gctcttggcc cagcatgatc ggatcatttt
     7921 ccagtttccg ttttactggt atagttctcc tgctcattta aaaatttggc aagataatgt
     7981 cttggaacga gcagcacatg tattaaaagg gaaagaacta ggaatcgtat tgacaacagg
     8041 tgtggcggaa aaagaatacc aagctggagg aaaagaagaa tatacgattt ccgaattttt
     8101 gaggccgtat cagcgaatcg caaataaatt ccatatgacc tatcttccgc cattcgtttt
     8161 agcacaattt atgtatttat cccaggaaaa acgttgggag aagctaattg cttatcagca
     8221 gtatctctca ttggaaggca aaccatcgct gactcagcgg attgactggt ttattcaaag
     8281 agtacaggaa aatcagaaaa tgcaggaaga agaatctgaa aaacaagcat atatcgtaga
     8341 agcactgact gaggcaaaag agcagatcga agacttgacc tttacactgc aagaaatgaa
     8401 aggaacttca ttatgacaaa tgattggctg atccaacaat tagatgaatt aagcgaaaaa
     8461 tcagaatatc aagaccgtgc gctcttatat gaaataaaga aactgatggc tgagatagac
     8521 gaacgaacaa tgcagctgca aggagaaatc gacggacaat cttggaatca tctcaactgg
     8581 tgaaattttc ttataaataa caaagataaa aatgtgcaca gcgacaatta tttgctacaa
     8641 taagaatatg aagcgtttga taacgctatt atacataaaa attggtggtg aaaaaaatgt
     8701 atgatgaaaa tgagtgggga gaaaatgttc ggatgacccg aagaagacag caaaaacagg
     8761 cagctcgtaa gcggatgatc atgattgggg cagcagttgc cgtgtttttg atcatagcag
     8821 gaggagttgc gggtctatat tttcatggaa aaaattcgtc agaagcgaaa agtacgacca
     8881 taacggaaac aacgacaaag aaaacagaga cgaaacaaac aagtgagtca tcaactaaaa
     8941 caaaagagac acaaacttct gaagaaaaaa cagcaaatgg tttgccagag caacttcaag
     9001 caatcgtaaa tgattattcg aaaaagatcg aagaaaagac gcctgcattg attgaagaat
     9061 accagacaga aattcaggga aatcaagagg gaatagctgg tttatcagca gttgctaacc
     9121 aaaaagcacg tgagcttcaa gcaatctccg acgaagggat cagaaaatta cgtgcagcgt
     9181 atcaggcagc agaaaataaa gacggtgtag acttagatac tttgatcaat caattatcgg
     9241 ctaattatac gaatcatgtc gctaagattt ccgatattta tttgcagact agcgcctctc
     9301 ttcaagcaga gtcaacaagc acacaagaca caacatcttc tgattctgag acagcagagt
     9361 cggtagaagc ttctgaaatg caagctcgaa caacacaaga cacaacagat tcctctgaaa
     9421 ctaataccaa cagcgcgact tcacaggctg cgacaaccgt agttttggaa ggagaaggtc
     9481 caaatcaaat agccgaacga actggcgtac cagtagaaac gatcttgtcg ctgaatggga
     9541 tgagcatgga gaattatttc ctgactcccg gtgagacgct aaaattgaat tgataaaagc
     9601 aataaaaaag aacgaagctt ccactaaggt gaagcttcgt tcttttttta ttttccggtt
     9661 cttttttcgc gtacaaagta atggatcgtc atgtaaagca agaaaacgag tccaataatc
     9721 aatgagattc tcgtttcagg attgataaac atgaaaatca atacgatagc taaaaaagca
     9781 agtgtcacat aattcgtata aggagatagt ggcattttaa aaggatgatc atgcatttta
     9841 tctgcgtgct tctttttgaa gcgaatctcg ctgatcaaaa tcactaccca aggaaccata
     9901 ccaggcaaaa tacttgagct gtatacgaaa acgaaaatat tttctgactg gttgatgaac
     9961 attggtagaa gataatttaa gatcagacca attaagattc cacctgagat tgctacgaca
    10021 ggataaaaag gcgcaccgtg acgagatagt ttcagaaatt ttttagacat ctttccattt
    10081 atggcaagtg tatagaccat acgacttgcg ctgaaaatcc ctgaattgca acctgataat
    10141 gccgctgtaa tcacgacaaa attaatcagt cctgcagcaa atgtaatgcc gactttagaa
    10201 aaagtttgta caaaaggtga accaatctca ctcaattgat tccacggata gatactcaca
    10261 atgataaaaa tagcaccaat ataaaaaatc aagattcgac cgatagtgga ctggacagct
    10321 tcaatgatcg tctttttagg attctctgct tcaccagcag tcattccgat cagttcgatt
    10381 ccttgatagg aagcaacaac gatcgataat gcaaagaaaa atcctttgac gcctccagtg
    10441 aagaatccac catttttcca aagatttgaa atccctactg cctgaccatg attaccaaat
    10501 ccaaataaga ttaccccaag acctgcaatg atcatcaaaa tgatcgttat gactttgatg
    10561 atcgaaaacc aaaattctaa ttcgccaaac gccttgacag aaataagatt tgccagcata
    10621 agaaacaaga cgacaatgat tccgggaata attgccggca aatcaggcca ccaataattc
    10681 atataagatc ctacagcaat cacttcactt attcctacga caatatattg aaaaatatta
    10741 ctccaagcgg tcaagtagcc agccacagga tggatgtact cgctcgcgta atttgcaaaa
    10801 gaaccagtcg aaggatcaac gtaaagcatt tcgcctaaag cacgcataat caaatacaaa
    10861 aagaatcctg caatcgcata ggctcccatc actgagggcc ctgtccaacg tatggttgag
    10921 gaggcaccca taaacagacc gacaccgatc gttccaccta gtgcgatcat ttggatatga
    10981 cgggcactaa gacttcgagt taacttttct tcttccatac aaaaactcct ttcgttgttg
    11041 agtcaacaag aaaaaataat caaacgtttc aacacatgtt tttcattatt aggttagcct
    11101 tattgaactc ttgatgtgat tctacagaaa attttcataa aatgcgagga aaattcgaaa
    11161 aaaacttttt catcattcta cttgaaaatg ctttccattt tagttaaact atatttaaat
    11221 aaaaagttag gtaaacctaa aaaggagtgt aagatttgga acgtacggcc attgtaattc
    11281 aagacctttc agccgcttat caagggaaaa ctgttttgaa aactatcaac ttgacgataa
    11341 atccacaaaa aattaccggt attatcggac caaacggtgc aggaaaatca acatttatga
    11401 aatctttgct agagttgata ccagctacta ctggtaaagt caccttttct ggacagtcgg
    11461 taaactcagt ccgtaaaaaa atagcctacg tggaacagcg aagtgaattg gatctttcct
    11521 ttccaattga tgtattaggc gttgtacttt taggaacata tccatcttta cgaattggac
    11581 aaagacctgg gaaaaaagaa aaagaacgtg caagacaagc tttgaaaaaa gtagggatgg
    11641 aagaatatgc caaaagacag atcagcgaac tatcgggtgg acagcttcag agagttttta
    11701 ttgcaagagc tctagcccaa ggagcagaat ggatcttttt agatgaacca ttcgtaggga
    11761 ttgatgcgtt aagtgaacga aagatctttg acatcttgca ggaattgaag aattcaggaa
    11821 aaacgatttt gatcgtccat catgatcttc ataaagtaga agaatatttc gatgaggtta
    11881 ttcttttaaa taaacagctg atcgcttccg gtccagtaca agaagtcttt acatcgaaga
    11941 accttcaatt ggcctatggt gagatcatca gacatttagt aaaaggagga gagaaaaaat
    12001 gatccaatcc tttattgatg gattaatgaa ttatcaattc ctgcaatacg ctttggtcac
    12061 ttccatgctt gtaggacttg cttctggagt catcggttct tttattattt tacgagggat
    12121 gtctttgatg ggagacgcta tctctcacgc agttcttcca ggtgtagcca tttcttatat
    12181 gtttggtttt agttacatct ttggagccac tgcttttggg atgctaacag cagcagtgat
    12241 tggtttcgtg actcagcgga gccgattgaa aaatgataca gcgattggaa ttgtctttag
    12301 ctcctttttt gcattaggga ttatcttgat ttcatatgcc cagagtgcga cagatttgta
    12361 tcatatttta tttgggaatg tcttagctgt tagggaaagc gatttgttgc tgacagcact
    12421 cgttagtgga atcgtcttga tatttgtctt tttcttttat aaagaattga agatcacatc
    12481 attcgatccg acaatggcaa aagcttatgg gctgaacaca tcgctgatcc attatctttt
    12541 gatgttcttt ttgacattag tggctgtagt cagtttacag acagtaggaa caatcttggt
    12601 gattgccatg ttgatcacac cagccgccac ggcttacttg ctaacgaacc atttactgaa
    12661 aatgatcatt acagctgcag gaatcggtat gctaagtgca gttgtcggtg tgtttttcag
    12721 ttatagttac aattggccat caggagctac gatcgtgtta gcatgtaccg cattttttat
    12781 ccttgctttt ttattttctc caacaaaagg aattttattt cagaaaggaa gcagatagat
    12841 gaaaacaaaa aaatcactct ttttaatctt ggcagttagt ttcttggtat tagccggttg
    12901 cggaaagcag gcttctgacc aagcggatga aggaagtaaa gaaaagttgt cagtcgtggc
    12961 taccaattcg atcttggcgg acatggcaaa agaggtcgga acagatcaaa tagatatcca
    13021 cagtatcgta ccagtcggaa cagatccgca tgaatatgaa gtactaccag aagacatcaa
    13081 aaaggcaagc gatgcagatg ttattttata caacggtttg aatcttgaaa caggtaacag
    13141 ctggttcgat aacttgatgg aaacggctaa aaaagaagaa ggaaaagatt attttgcagt
    13201 tagcaaaaat gtagaacctc tttatttaac tagcggtgaa gaacatacaa aagcagatcc
    13261 ccatgcttgg ctagacctat ctaacggaat aaaatatgtg gaggaaatcg cacgtatatt
    13321 ctctgaaaaa gatgcagaaa atgcgacact ctataaaaaa aatgcagaag catatgtgga
    13381 aaaactaaaa gaattagata cacaagcgaa ggaaagtttt gcttctatcg aagagaacaa
    13441 aaaattatta gtaacaagtg aaggggcatt caagtatttt tcacgagcat atgatttgcc
    13501 agcagcttat atatgggaga tcaatacaga aagtcaaggt acgcctgatc aaatgaaagc
    13561 gatcattgat cagataagag caaaagaagt accggtttta ttcgtggaaa ccagtgtaga
    13621 ctcaagaagc atggaacggg tagcaaaaga aacgggtttg aaaatctatg ataaactgtt
    13681 cactgattcc atagcaaaag aaggagaaca aggagattct tactatcaga tgatgaaatg
    13741 gaatattgaa acgatccatg aaggtttgtc acaaacaaaa gaaagctgaa aagtgcaagg
    13801 tctagcttga ggagcagacc ttgcattttt tataagagat atgaaaaaaa attatgctgt
    13861 ttttcgtcgt acagatacct tttttgaaga aattccgtta tagttagagt aaggaaggaa
    13921 ggtgtggcta tgaattttac acgtaaagat aaatatacga ttaaagaaga agtctttcga
    13981 ttgatcagcc agcaggaaaa taccgacaag gatcaagacc ttcaatttct tgaagcagaa
    14041 ttagctaaat tattcatagc aaatcaaaat gagacaacag aaccagatga agagctgtac
    14101 ataggcaaat ggttgtttga tgaatttcag cctttagatg aaaataccgg tatcggttat
    14161 tttgtgttcc tttctccagt cggcttcaca cctggcgtat tagttaaaca aaacgacgaa
    14221 ataaccgtca aagcatggcc tgcattattt tcagtcatca aagcagaagg agtaatcaaa
    14281 aagatccagt cggcctatta taaaagtcaa tttattgcca acagcaaatt aatgaatatg
    14341 ttttataatc gtctagtaga aattgctagg cctgggaaaa tcccgcaaac attcacgtac
    14401 acacagcaaa aaaatctcta tgaagaagta ttgtattccc gcgatctgtc tgagttttcg
    14461 ccatgggaag aggtaaggct ccatgaactg ttaagtacaa aaccagtttc attgaatttt
    14521 tttaatgcat tagataacaa aagaaaatta atttcaacaa attattggga agtatttgct
    14581 gcaagaaaaa aatggatgga agaaaagaag attgccgatc agcagatcca gtttgtccag
    14641 ctgaaaaaag aaaaactata ttatgggtta tacagccgtt aacagcaagc gaggaagacc
    14701 atgacattaa aagaagaaat catacaagaa agcaaacgat taggaattga caaaatcggg
    14761 ttcaccacgg cggaaccttt tgattcatta aaagaatcgt tggaagaaca aaaagcagct
    14821 ggccatactt ctggctttga acatccaaat atcgatgagc gtctttatcc agaaatgaca
    14881 tttgatcagc cgaaatcgat catttccatt gcacttgcgt atcctactag gatccatgaa
    14941 gaagtgccta gggatgaaaa acgcgggcag tttgctcgtg cttcttgggg gatcgattac
    15001 catgatatac tcagagaccg tttgaaccga ttgatcgaat tcatccaaac aagagcagaa
    15061 aaatggcaaa aagaagagga atggcgtcta gctccgcaag tagatactgg agaactgatc
    15121 gatgtcgctg tagcacagcg agctggttta ggatttattg gacgaaacgg attgttgatc
    15181 actgaagagt tcggttcatt tgtctatctt ggtgagatcg ttactaatat tgtatttgag
    15241 cctgataaac caggagaatt tggttgcggt gactgtatgc gctgcgtgac tgcttgtccg
    15301 acaaaagcat tgcttggtga cggaagaatg aatgcccagc gatgtttgtc ttatcaaact
    15361 cagacaaaag ggatgatgcc ggaagaatat cgtaaaaaaa tgcgaaatgt catttatgga
    15421 tgtgatatct gccagttagt ctgcccttac aatcaaggaa aagattttca ttttcatgaa
    15481 gagatggaac caaaagtaga cgaaatatat ccaaaattaa agccaatgct ttcgatgtct
    15541 aataaagact tcaaacagca atttgggcat ttagctggtt catggcgtgg gaagaaacct
    15601 ttgcagagaa acgcattgat tgctttagct aatctaggtg atcgaacggc attgcctgag
    15661 atttcagaat gtttaacaga tgtccgccct gtcatcagag gaacagcagc ttgggctatt
    15721 ggacgtctag gaacaaaaga accggaaaaa tggcttgatg tattagatga aatattagct
    15781 aaagaaacag aagaagtcgt tataaacgaa ttggaacatg cccagaaatt attgcggaga
    15841 aagttaaaat aaaaaaatag ttaacattat tca
//
LOCUS       DACEMO010000050        15847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEMO010000050 DACEMO010000000
VERSION     DACEMO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.051"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1289)
                     /locus_tag="IW728_001192"
     CDS             complement(<1..1289)
                     /locus_tag="IW728_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1024566.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIR"
     gene            complement(1471..2700)
                     /gene="rpsA"
                     /locus_tag="IW728_001193"
     CDS             complement(1471..2700)
                     /gene="rpsA"
                     /locus_tag="IW728_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1024567.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(2835..3515)
                     /locus_tag="IW728_001194"
     CDS             complement(2835..3515)
                     /locus_tag="IW728_001194"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1024568.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(3564..4199)
                     /locus_tag="IW728_001195"
     CDS             complement(3564..4199)
                     /locus_tag="IW728_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1024569.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4265..5668)
                     /locus_tag="IW728_001196"
     CDS             complement(4265..5668)
                     /locus_tag="IW728_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1024570.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(5655..6686)
                     /locus_tag="IW728_001197"
     CDS             complement(5655..6686)
                     /locus_tag="IW728_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024571.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            6717..6938
                     /locus_tag="IW728_001198"
     CDS             6717..6938
                     /locus_tag="IW728_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1024572.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7103..7840)
                     /locus_tag="IW728_001199"
     CDS             complement(7103..7840)
                     /locus_tag="IW728_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1024573.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(7993..9333)
                     /locus_tag="IW728_001200"
     CDS             complement(7993..9333)
                     /locus_tag="IW728_001200"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1024574.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(9570..11093)
                     /locus_tag="IW728_001201"
     CDS             complement(9570..11093)
                     /locus_tag="IW728_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1024575.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(11471..12082)
                     /locus_tag="IW728_001202"
     CDS             complement(11471..12082)
                     /locus_tag="IW728_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1024576.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12205..12330)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(12412..13128)
                     /locus_tag="IW728_001203"
     CDS             complement(12412..13128)
                     /locus_tag="IW728_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1024577.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13134..13703)
                     /gene="scpB"
                     /locus_tag="IW728_001204"
     CDS             complement(13134..13703)
                     /gene="scpB"
                     /locus_tag="IW728_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1024578.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(13687..14484)
                     /locus_tag="IW728_001205"
     CDS             complement(13687..14484)
                     /locus_tag="IW728_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1024579.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(14454..14825)
                     /locus_tag="IW728_001206"
     CDS             complement(14454..14825)
                     /locus_tag="IW728_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1024580.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(14844..15731)
                     /gene="xerD"
                     /locus_tag="IW728_001207"
     CDS             complement(14844..15731)
                     /gene="xerD"
                     /locus_tag="IW728_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1024581.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4439 a         3454 c         2686 g         5268 t
ORIGIN      
        1 attcggatgg acgtcccttc aaaagtaaat gcttttctga tttgattttc taagaaacgt
       61 tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa cgacaaatgt aggtggtttg
      121 acagcgactt gtgtagcata aaaaatcttc aggcgttttc ctttatctgt tggtgttgga
      181 ttgattgcta cagcatccat aatgatatca ttcaacacag ccgaagagat acgaagattc
      241 tggttcatac ttacttgttc gatcatttca ggcagacgtt ccaaccgttg tttagtcact
      301 gcagaaacaa aaacgattgg agcgtaatca agataccgga attcatcacg aatctcttgt
      361 tcgaaatcac gcattgtatt ggtttctttc tttacaagat cccatttatt cacgacgata
      421 ataattccac gaccagcttc atgggcgtaa cccgctactc gcttgtcttg ttcccgaatc
      481 ccttcttcag cattcagtac catcaaaacg atatcagaac ggtcaatggc acgcattgca
      541 cgcatcacgc tgtatttttc agtattttcg tatacttttc cgcgtttacg cataccagct
      601 gtatctatca ttaagaattt ctgaccactt tcagaaacga agtgggtgtc gattgcgtct
      661 cgggtcgttc cttcaatatc cgaaacaatt acacgttctt ctcctaaaat cgcattgatc
      721 aaagaggatt taccaacatt tgggcgacca atcaaactaa acttaatcgt gtcgtcgtcc
      781 tcttcttcag tctcttcaga gaaatacttc actgcttcat ctaaaatatc accaatccct
      841 agtccatggc ttcctgaaac aggataagga tcgcctagcc ctaaggaata aaattcatag
      901 atatcgtttc tcatttctgg attatctact ttattgactg ctaaaatcac tggtttatta
      961 cttctgtata aaattttcgc aaccatctca tctgcgtctg tcacgccttc gcgtccgctg
     1021 actacacaaa tgattacatc tgcttcttca atcgcgattt ctgcttgatg cttgatctga
     1081 tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt cgatcacgct gaattctcga
     1141 cctaaccatt caccttttgc ataaatacga tcacgagtta ctccaggagt atcttcgaca
     1201 atcgagatac gctcaccagc gatgcggtta aaaatcgtag acttaccgac gttcgggcgg
     1261 ccaacgattg caattgttgg atttgccata ttatttcctc ctcacaagtt tccaatctca
     1321 cttagtttaa cgaatgcatt gccaactttc aagcatagat tgtcaattaa acgaagtatt
     1381 tttattcctt attgaaatac taatcgcttt tcaaaagaaa aactgagaca aaagcagctg
     1441 ctttcatctc agtcacagat acaaaatcaa ttatttttca gaatcatcgg ttgctttttc
     1501 ttctacttct tctttcaacg catcacctaa aatgtctccc atagtaaatc cagtattttc
     1561 ttctggtaat tcataagact caacattttt tggttcttct tctgaagctg gtttttcttc
     1621 taaagctttg atactcaatg caatacgatg ttcttctgga tggatctcta atactttcac
     1681 tttcacttcg tcaccttcat gaagcacttc atgtggtgtc gcaatatgtt tatgtgaaat
     1741 ttgagagata tggactagcc cttctactcc agggaacact tcaacaaagg caccgaagct
     1801 tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact ggagcttttg tatcgatttc
     1861 tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa acacgttctt gttcaggatt
     1921 gatagacaac actttgacat ttacatcatc attgacagcc aatacatcac ttggttttgc
     1981 tacatgacta tgtgaaatct ctgagacatg gactagaccg tcaattccgc ctaggtcgac
     2041 aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg agaacatctc cctcatggat
     2101 agttgaaaga atttcttttt tcttttcttc tctttcgttt tccactaccg ctttatgcga
     2161 taagatcaaa cgattttctg atggttcgat ttcgatgatt ttaaagctca atgttttgcc
     2221 tttatagtct gaaaagtctg caacaaaatg atcttccacc attgatgcag ggacaaaacc
     2281 acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca tttgtaacag gtgcttcgat
     2341 gaccttgccc tcttggaaat ctctttcgat atcttcccaa acttttttag catctaaacg
     2401 gcgttttgaa agtaggtagc tgccgttttc tttatctttg ccgatcgtac tgataacgac
     2461 taaatctaat acatcaccga cttttactac ttcgctgata tcttctaccg gcaaagttga
     2521 taattctttt gccggaacga ctccttcaac tccagcccct tcgattccga caatcacttg
     2581 tttgtcttca atgactaaaa cttcgccctt aacaacatcg ccaactttta cttcttgtac
     2641 gcttttcatt gcgtcttcca ttgactggtt gttctcttga ttttgatcaa attctgtcat
     2701 aacacttgtc ctcctaacca acattctgcc gtaagtacgg tactgttttt agtttaattg
     2761 attattcaaa aaaaataaag cgatttcgtt cgttttcctt gaaaaaacaa ttgtttttca
     2821 ttttttttct taatttaaaa aagaagataa ccttttttca aaacaacagc ttttattgct
     2881 gcaacgactt ctggaatcga tttgccagtc gtatcgatac gaacagcatc ctctgcttgc
     2941 ttcagcggag agacgtccct tgttgaatca taatggtctc tttcctgtat ctctttcgtc
     3001 agtgtttcaa gatcagtcgg aattcctttt tcttggttct ctttgaaacg tctttctgca
     3061 cgctcactta cgcttgctac taaaaagatt ttcacttctg cattcggtaa tacagctgtc
     3121 ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc ctatttttcg ttggacagca
     3181 actaattctt ctctgacttt tccatgtgca gagacttcag aaacagcttt tgtgacatct
     3241 ggttgacgga tttctaaagt cacatctttt gatgctatga atacatgctg tccatcttct
     3301 tgttgcgaaa aagagatagg atactttttg atcagcgaaa ctagcccttc ttcatctgaa
     3361 aaagatacat tgttcgtaat agctaaataa gttactgcgc gatacatggc accggtatct
     3421 gtataaatat aattcaattc ttttgctaat atcttggcaa cggtactttt cccagatgaa
     3481 gcaggcccgt cgattgcaat actgatttgt gtcatgatat aactcctttt tctcagaaaa
     3541 aaagccgcaa agcgcggcta ttattattta attcttaatt cttgaccagg gtaaagcata
     3601 aagttgtttg gatctaagcc attcaattca aacaattgat cagtggtgat ccctgcacgc
     3661 tcagccactt gttttggacc ttcgcctgct tgaacagtcg ttgttgaacc ggctgcagct
     3721 gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat cccctgcttg ataagttgcc
     3781 tcacttgagg aagtcggtgt tgtctctgct gcaatactgc tttctgacgt tacacctgta
     3841 tcttgagaca cagtagattc ttccgcgatt gtactagatt ctactgttga agtctcagtt
     3901 gactcctctt ttgttgtact tgtagaactt tctactactg atgtcgaagt ttcagaagaa
     3961 gttgctgctg ttgcattgtt gcttgatcca tttctgatcc agaaataagc acctgctgga
     4021 atagcgatac ataagaccag taaaatcaaa aatagcgaaa gaaataatgt atttcctttc
     4081 ttttgttgtc tctgagaact tcttgatgaa acttcatcat ctgtatcgta gattggttgt
     4141 tcccatggtt cttgtgtttc gttattttct gtagattgat gtctatcttt tctgctcact
     4201 gttttacctc ctaaactcta tcgtgtgtat tataccatag gaaaaagtgt cttgtcctaa
     4261 aaaatcaatg ttccatgttt tttttaaata aatttagtaa aatcgtttcc caatgaagtt
     4321 gttcagtctt agaaaaaagc ttcctctttg gcagaagttc tgcttttgct ccatcaatat
     4381 cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa ataagctaag atgaattttc
     4441 tgcggcagtt ttcttcatta atatagcgaa gcatctgctg caggcggaat tctttttctt
     4501 tttcctgacg tttgagccgt tccacccaat tttctggttt ttcagactgc tggatcagct
     4561 ccatccattt tttttgcagc tcatcaaagg gagcttgctt tgtggcatat tccaaataaa
     4621 gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa gaaataatgg atacgttcat
     4681 ccccactttg ataaaaaaga attgctgcac tttcttcctg atctctgcct gctcgaccga
     4741 tttcttgtac atagttttct agactgtctg gcaaatcata atggaccaca taacggatat
     4801 cagatttatc gatccccata ccaaatgcat tcgtagcaat gagaaactgc agctgatttt
     4861 ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc aagaccaccg tgatagtatc
     4921 caactgtaaa acgttccctg aataaatgat acaatcgttc gacttctttt tttgttgcac
     4981 aataaatgat tgccgctcct tgtgctcttt ccatgaagtg ttcaagttct tgttcttttt
     5041 gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt gactgattgt ctgatctctt
     5101 ttggtggtcg atcaaatagt acatgacgta tatctcttgc tactaaatct gttgctgtcg
     5161 ccgttagtgc cagtgtgacc gggtttccta aaagctttcg gatttttcct aactgttgat
     5221 attctggacg gaaatcaact ccccattgag acacgcaatg agcctcatct actacatata
     5281 acgcaatatt ttgttttttc agatgctcta aaagagaagg attagtcaac atttcgggac
     5341 tcaaaaacaa aaatttatat tgcgatagat gacggaggac gtatcttctt tcagattgcg
     5401 aaagtaaact attgaaagca gctactcgtt tctcccctcg tttttgtaat tgcgtcactt
     5461 gatcttccat tagagaaagc agcggtgaca cgatgatgac cagaccttcc attaaatacc
     5521 ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg caaaatagcg agagtatctt
     5581 ctccatcaag caacgcctga ataatttctt cttggccggg acgaaatgaa tcaaatccaa
     5641 aatatttttt taattcattt attaacattc tgtatcgctt cctttctcaa tagatagatt
     5701 tgatacaaac gaaattctcc ataatctaat tgtccatcta cattccattc gctaaagcgc
     5761 cactctcgca cggaatcctt atttggttcc aaacgttcga ttgtttctgc agagagaatc
     5821 cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa taagatgatc tgttactgtt
     5881 cctcttttta aatgacggat tgccatgatc tggtcaattg tctgaccatt cataaacatc
     5941 tgcttggtga tcatcatgct ttgattgaaa ttttgctgta ataatgaatc gatcaaagcg
     6001 tgccaaagcg aatctggccg tttttcgatt tgtgctaaca aaaggtggat acatctgctt
     6061 tgttgcagat aaacctctga ctcatcattg ttttttgcca gctgataagg aagcagccct
     6121 gttcgctcat ttccagataa ttggttagct aaaaaatctg ctgcttcttc tggtatctcc
     6181 gaaaacagct gcaccaaact ctcatacgca gtatcaatca aaatttcacg tggcaattgg
     6241 ctttcactaa gccatttttt tagttggaaa gtataaaaag gacttgtctc tgctggaaga
     6301 tagtcttgaa tacccgttgc cagattagaa attacttgaa cagcaaattt aatgagccgc
     6361 caactagttt cccctgttct gccatagcgg tcaaaacgca aaccagtatg ttctatttga
     6421 tgttcggaca acatatcagc ccctgcaggc gttagctttg cttcattttc atttatttcg
     6481 atccaacctt gctgcgcaat tttttgcaaa atttgataaa acttatcctg cttcaattct
     6541 ggaaaactgt tatggataaa caaaagttcg tgggtaaatc cataaataag tactgaagtc
     6601 gttcgttttc ccacaaggag ttggtaaaga gaactaatcc gcagcttgtg tcctgatgaa
     6661 aatagcgata aaataaaaac aatcatatta ttctccttaa gttaggatgg ttagaaatgt
     6721 cactactatg taaacttgta cctgaacgct gtattgcatg cggtctttgt caaatcgaag
     6781 cgccagatat tttcgattat gatgaagaag gaatcgtttt atttgcccaa gaaccagagg
     6841 ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt aaaaaaagcg tatcaaaaat
     6901 gtccagttcg agcgatttta ttagaaaata aaatataaat ataaacgaaa ctttaaaaac
     6961 gtacactaag ttctctaaac ttgaaaataa gccgggatct gtttctcttg ttccgtgaaa
     7021 tcccgactta ttttgaaaga ttcatttgct atctatagtt aattgactag ttttttcacg
     7081 gaccctatag gacccattta tattatttca actttttcag ccagttttcc aatgtattga
     7141 cgatcacagt ttgctggtct gcgttagaaa tttctgcctc tccatctcct ttttgctcac
     7201 catagctgcc aaaacctcca tgatttccgc cattgattga tacatactct gttctttccg
     7261 gtaaatactg cttagcttct tgataagctt cctgattcaa tactttatct tttgtcgccg
     7321 tgatcgataa aacaggaaca gctgattctg tcaatgttcc ttttttgtcg ggataacttg
     7381 ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt cttattgaca taacgacttg
     7441 ccattacgcc acctaatgaa tggccaccga tcacataatc ggaatttgga aaatcttctt
     7501 tgaccttatc cgctttgttt ccgcccatca ctgctagatc aagtggcatt ttgacaatag
     7561 cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat actataactt tctggctcta
     7621 cgagagcacc aggataaaaa ataagtaatg gagactttcc atgattcttt ttctctcctt
     7681 caaaaaataa gtaatcttct ttgttttttc cttcttttac caacacttgc gcttctgacg
     7741 atggttcgta cgtattttgc tgtatatagt aataaccggc tccaccgagt aatcctaaaa
     7801 tgatgactaa gccaattagt attcgtctaa atattttcac tttctcccac cttttctatc
     7861 tgagtgaccc ctttttctta atcaagtatg attatacctt aaaaaaataa gagagaaatg
     7921 caaactttcc agcatcaaat aaaaaaagac ggaatatcca tttgaatatc cgcccttttt
     7981 gtatgtttat ttttatttag cacataaacg atacacagct tctgcataaa tttccattgc
     8041 tttatacata ctacttaatt cccatcgttc attcgcctgg tgcataaagt caggagtatc
     8101 agggaacatc gcaccgaatg ccacacactg gttcatcgta cgagcaaaag tagcgccgcc
     8161 tgatacaaac ggttcagtca tgtctccagt aatatcgcga tatgtcccca atagattttt
     8221 cactaactca ctgtctagtg gcacgtataa tgaatcaagg aagtcaaatt cttcataagt
     8281 caacccatat ttttctgctg ttttagttaa tttagctact aaatcatctt tcttgaatgt
     8341 aacagggatt ctcatatcta caccgatttt tgtttgttct ggtgtgattt ccaaacttgc
     8401 aaagttcatc gttagctcgc cggattgttc atcttctgtt ttgcccacga cagcttcacc
     8461 agttgcattt tcttgaacta attttcctaa gaaattgatt gggccaaaat caaatacttc
     8521 tgaaagagca attgccagac gagaaatagc attcactcct tgtgctgcat ctttcgcgtg
     8581 gatactttta ccaagaacga cgatgccttc accttgttct tcgaaatcga attcgtgttt
     8641 tttcaatgct tcttttactt cagaaagctt ttctccagag taaggagctg catcaggaac
     8701 aacattcaac gctcctccag cttttacaga aaactcgttc gttcctggtc ctgtcaagta
     8761 agcttgcaat aagccttttt ctgcataaat taaaggaaat tcagcatctg gcgcaaatcc
     8821 ttgcgtaatt ccttcttctt tttcgttata tttttctaaa caacgccata aattttcttc
     8881 atctgtacca aagataaaac gaatacgtgt cttgaattcc acgccagcat caatcaaagc
     8941 tttgacggca tacattgccg caatagaagg acctttgtca tcttgggatc cgcgaccaac
     9001 taaccagccg tcttttatag ttggatcgaa tggttttgtt tcccaattat tttcgtcccc
     9061 tgctggaaca acgtccatat gacacaagat accgaataat tcatctccag agccaatctc
     9121 tgaataaccg taatagcctt ctggatcttt aaatgttcta aatcctagct tttcactgat
     9181 ttccaatact ttatccaaag cttcaatcac ttttttaccg aagggatgtc cttgtcctga
     9241 atctgcttca tctagtacgg aaggaattcg gatcaattcg ctcaatgatt ctaatgcttg
     9301 ttcatgatgt tgttctgtca caaatttttt catgatatta tcctcctaat atgttaaaaa
     9361 gggtcatgtc aaattcctcc gacacaacct cttcttctca aataatgacg ttttaagcct
     9421 gctccatcag caagcagaat gttcttaaca aatgatgaaa gaaaaaacac tcactgaatg
     9481 gagctattga cttgtccacg tgcttatcga tagccgttta tcggtttctt cattcttatg
     9541 atagaccttg tacctgtatt tgtctaggtt tagaacattg ctgctacacc taggatcaca
     9601 cagatggcta caaataatat cgcaattaat ttaccggtaa atttccacca cactgaaaca
     9661 tcgaaacgag caattgctac agcacccatt acgatagcag atgttggtgt aattaagttt
     9721 actaaaccag atgcagattg ataagcagtg attactaaat ccttaccgac gccggcaaat
     9781 tcacccatag gtcccatgat tcccattgtt gcagcagcta agccggaagt tgaaggaatc
     9841 aagaatgaca tagggatata gaaaataaat gtaataatga tgaatacact tgaagataat
     9901 gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg tgattaatcc attgttcata
     9961 accacttgga taccacgagc aacagctact acgattgcta caccgatcaa atcagaagca
    10021 ccagctaaaa attcagagac aaattcagac tctttcatgc cataagtgat tgctactacg
    10081 atggacatta ctaagaataa catagtaatt tctgggaagt accagtcacc taatggaagc
    10141 atgcttttac ctagaaccgt acctagtacc ggaaggtttg ttaaccagtt tgtcaatgat
    10201 tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa tcaaactgat gatcatgata
    10261 ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt ttttcgtgat tacttcttga
    10321 cgaccgattg cttcaatatc gaaatgcttc atatcttctt tacgttggtt gtatactaat
    10381 gattttgttg gatctttttc gatctttgat gcataacgat acacatatac aatagaaata
    10441 gctaacaaaa taacgaacat caagaatcga agaccaatac cttcacccat cgaaatccca
    10501 acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg ttgaagctag acagcctact
    10561 tgagacccta ctaatacgat tgctacagct actactgtat caaagcctac agccatcatg
    10621 actgggatca gtaaagcata gaatggaatc gtttcttctg ccattccgta agtagagccg
    10681 cctaatgcaa ataacagcat caatactgga ataagcattt tttcttttcc tttgaatttc
    10741 ttcacaactg aagcaatccc ggcatctagt gcccctgttt tgttgataat acctaaaaat
    10801 ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag caggtgtttc tttagttagc
    10861 tcccccgctg gtacacctag catcccattg attggcgcca tgaaaatatc ccacagccct
    10921 tgaggatttt gttgtactcg ttcatatgat ccagcgacga tgttgcctgc atcgtctaca
    10981 ctatagtgcc cagctggtat aaaccaagta agtacagcga ttactgcaat aataataaat
    11041 aagaccgtat acgctgaagg catttgaaat tttttctttt ttgtttgttc catcttcttc
    11101 ttcctttctt ttccgttaga gacaacaatt ttggctctaa tgttatttta tcaaaaagag
    11161 tatcagaaca aaaataataa cacgcatttt gtaaaacttt tgttcaaaaa gttcgttttt
    11221 ccctattccc tcctcttttt tcaggttttt aaagctttct tctttttcat tgtagcgttt
    11281 cctttttttt ataaacaaac attttcgtct tttaaaagga gaagaatttc acatttgcaa
    11341 agaattgatt gatttttatg cataaatcaa aaaaatactc aaaacaaaca aataaaacgt
    11401 ttacattatt gcatgtcttg tatttttttc caaaaaaaaa gaccaagaaa tttcttgatc
    11461 tttttgatat ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta
    11521 accatggaag aagttgcgca tgcagcacta aaaagacagc actcacgatg aatcctttga
    11581 tcaagttgaa aggaacgatc ccaatcagta catagtttgc taaagacatg cccagcatct
    11641 gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga
    11701 tactcataaa aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc
    11761 ctttatgctg tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg
    11821 ataagaaact agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat
    11881 gtaatactga acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc
    11941 tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg
    12001 gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa catcgctact gcaaccattt
    12061 tctgtacacg ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt
    12121 tttgacgttt ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta
    12181 tttgatcaag cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc
    12241 ggaatttcac cgattcagct atttttttgc aaaataggtc gtggactata accaccggtc
    12301 gggaatttca ccctgcccct gaagacgaac cttatataat ttttgtttac atattcatta
    12361 tacatatcta ttcaaaaaac gcaacatcta acacttataa aaagtaagtg ttcattttga
    12421 gtggttcagc aaactgctga cttctttttt gttcagttca cggtaatccc caggacgtag
    12481 gccttgtaaa gtcagatctc cgtatttttc acgttttagt ttttgcacag gaagaccaac
    12541 tgcttgaagc atatttttca cttgatgatt acgtccttcg tggatcgtca attccacgat
    12601 actgtggttt gtcttaggat ccactgataa aatttggaat ttagctggag atgtttttcg
    12661 tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt
    12721 tacctttgca acgtaaactt tatccacttc atgttttgga tgtgtcaggc gctgagcaaa
    12781 atctccatca tttgtcagta gcaagatccc cgaagtatcg taatccagtc gacctacagg
    12841 atagatacgt tcttttacca tcggcaaaag atccgtaaca acttttcttc ctttgtcatc
    12901 tgcaacagca gaaataactc ctttaggttt gtacaataaa tagtaaccgt attcttcttg
    12961 ataaattggg acttcatcca cttccacagt gtcatgtttc ccaactttta tccctaattc
    13021 ccttacaact tgtccgttta cttttacacg tcctgcaagg atgaattctt ctgcttttcg
    13081 acgagaagtg atgccagcgt gggcaattac tttctgcaat ctctccatta caattattct
    13141 cctttattca ttccatcaaa gaatagatct ggtgacacgt cttcttcaag tgaaatttcc
    13201 atttcttgtg tatcaggcag ttcttctaat gatttcaaac caaaataatc taagaagtag
    13261 ccagttgttc catataatat cggccgacct ggtccttcta ctctaccttt ttcttcaatc
    13321 agtttatggg cagctagacg ctgtaccgct cctgaaacct gaactcctcg aatctgctcg
    13381 atttcagctc ttgtgattgg ctgtttataa gcgatgattg ctaaggtttc gacggcggct
    13441 tgagataatg tgtttgatgt cccttgcgca tatcgcttaa gtaagggagc cagctctttt
    13501 ttagtcgtta atacataatg gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt
    13561 tgagtgtaag attcttttaa ttctttcact aaacttgtga cttctgcttc gctgagattt
    13621 aacagtccgc ttaattcttc attactgatc ccttcctcgc caacaataaa aagaagggct
    13681 tcaagttcac ttaatttcgt catgttcggt atctcctact gctggataaa gcataattga
    13741 ttcatagttt tctttttgtt gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc
    13801 cataaaagta gtgacaactt cacttttaga aggtgactca aaaaaagaat cgaaagtaca
    13861 gccatgcgca tttcttttta tacgctcttc gataaatacc attcgttctt cgatcgttgt
    13921 gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat ttcttacgtt ctaagatttt
    13981 ctgcatggca cggatcaaat cgattttttt gacttgtcct cgcttcaacg gcttttcagc
    14041 atccgctaac ccatcgatgt tcataggttc ttttgtgtaa taaagacttc gttcctctgc
    14101 tttttctgtc agtctttcag ctgcatattt atatttacgg tattctaaaa gctgagtgac
    14161 caattcttct cgaggatctt cttcccataa ttcttcatcg tctgtgccga attcttggac
    14221 aggaagcaat gtcttgcttt tgattgccat caatgtagca gccatcacta agtattctcc
    14281 tgcaacagct agctcgagtg ttttcattgt atggatataa ttcatatatt gctccgtgat
    14341 cgtagcgatc ggtatatcat atatatcgat ttccatttgc tgaatcagat gaagcagcag
    14401 atcgagagga ccctcgaata catctaattt aatattgatc tccgtcaact cattcactct
    14461 ttcttctttt tctttgctag ccattttcca atgtaaggct ctgtgatttc agttgcacgg
    14521 atggagacat ccgggtactt ttcctgtaat tcatctaaca tttgaaagcc catattctca
    14581 ccgcgataag acggattcaa agaaatgtca tggatgatca gctgatcgtt cgttgcccag
    14641 taaataccaa taatcccttg aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca
    14701 taatcgtctt cagattcgta cgtatcaatc tccttcaaca aagaagtttt acctgtcagt
    14761 ttcttatgga aactcaatag tcccatagca attttacggt tttctttccg gtaatgtgtt
    14821 aacatcttct caccttccta tgactaagcc cttggaaaat gctgcttata aacatccgtc
    14881 attcttttct tcgtgatatg tgtataaatc tgagtcgtag agatatccgc gtgacctaac
    14941 aattcctgta ctgtccgcaa atcagctcca ttttctagca gatgtgtggc aaagctatgc
    15001 cgcagcgtat gtggtgttac ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga
    15061 tttttccaga ttccttgccg agaaagacct ttcccatgat tattgacaaa tacatgcatt
    15121 tcagatgcgt cctttacaag taatggacgt gcttcgtcta agtagcgttc cagccattgg
    15181 atcgcataat cacctaaagg aatgatccgc tctttatctc cttttccaac tgtctgtacc
    15241 agtccaagag aaagatgaag atctcccaat ttcaatccaa tcaattcact gacgcgcatc
    15301 ccagtagcat acatgacttc caagattgca cgatctctta tccctaacgt ttttgtcgta
    15361 tcaggtgctt caatcagctt ttctacttct gtcagagtca atgtacttgg taatttctgt
    15421 gctttcttcg gcgtatcgat atgttgcatc ggatcatgat cagtgatacg ctcttgccgt
    15481 aaaaactgat ggaatcttct gagacttgag atcatccgag taacagttgc tgtcgctttt
    15541 ttttcttcat gcaacttctc cagaaaacgg atgactaaaa agcgatcgac atcctgccat
    15601 gaagcaattt tctgctcctg tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt
    15661 tgtcgtgtat tggcggacaa tccccgttca atattcaagt agtggagata atcgttgatt
    15721 tgctctttca taataatccc cctcatgcct ctctatcata tcagataccg cttcattctg
    15781 atagtctttt tccataatct gagcaaagaa aaagccgtct agacactcag acggactggt
    15841 ttctttt
//
LOCUS       DACEMO010000051        15618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEMO010000051 DACEMO010000000
VERSION     DACEMO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.052"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            292..1431
                     /locus_tag="IW728_001208"
     CDS             292..1431
                     /locus_tag="IW728_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1024582.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IW728_001209"
     CDS             complement(1498..2073)
                     /locus_tag="IW728_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1024583.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IW728_001210"
     CDS             complement(2078..3052)
                     /locus_tag="IW728_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1024584.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IW728_001211"
     CDS             3335..4303
                     /locus_tag="IW728_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1024585.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IW728_001212"
     CDS             4416..4694
                     /locus_tag="IW728_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1024586.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IW728_001213"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IW728_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1024587.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IW728_001214"
     CDS             5160..5906
                     /locus_tag="IW728_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1024588.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IW728_001215"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IW728_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1024589.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IW728_001216"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IW728_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1024590.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IW728_001217"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IW728_001217"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1024591.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IW728_001218"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IW728_001218"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1024592.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IW728_001219"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IW728_001219"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1024593.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IW728_001220"
     CDS             10643..11185
                     /locus_tag="IW728_001220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1024594.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IW728_001221"
     CDS             11208..12764
                     /locus_tag="IW728_001221"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1024595.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IW728_001222"
     CDS             12778..13680
                     /locus_tag="IW728_001222"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1024596.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IW728_001223"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IW728_001223"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1024597.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IW728_001224"
     CDS             15123..15545
                     /locus_tag="IW728_001224"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1024598.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5170 a         2826 c         3384 g         4238 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaaga
//
LOCUS       DACEMO010000052        15435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEMO010000052 DACEMO010000000
VERSION     DACEMO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.053"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..239)
                     /locus_tag="IW728_001225"
     CDS             complement(<1..239)
                     /locus_tag="IW728_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024599.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAK"
     gene            complement(239..445)
                     /locus_tag="IW728_001226"
     CDS             complement(239..445)
                     /locus_tag="IW728_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024600.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLAQVYELVTIDHEEVE"
     gene            complement(442..639)
                     /locus_tag="IW728_001227"
     CDS             complement(442..639)
                     /locus_tag="IW728_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1024601.1"
                     /translation="MNNRHRRVTKLRKQELNALKAKFEKEYGISIEEAYKVASQCVAD
                     ASEAIRKFGISILDIKWEDTE"
     gene            complement(646..876)
                     /locus_tag="IW728_001228"
     CDS             complement(646..876)
                     /locus_tag="IW728_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024602.1"
                     /translation="MKYEIALSEADVQSIINGRKVKFKLSDGTEIVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            complement(899..1204)
                     /locus_tag="IW728_001229"
     CDS             complement(899..1204)
                     /locus_tag="IW728_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024603.1"
                     /translation="MNVQVYLKRDLARFPRSWDEYSCPFEAEEESYLMSANMIEITRD
                     DADEVDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            complement(1201..1398)
                     /locus_tag="IW728_001230"
     CDS             complement(1201..1398)
                     /locus_tag="IW728_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024604.1"
                     /translation="MKAYTIKFYGNKTFVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            complement(1401..2028)
                     /locus_tag="IW728_001231"
                     /pseudo
     CDS             complement(1401..2028)
                     /locus_tag="IW728_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337468.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
     gene            complement(2021..2098)
                     /locus_tag="IW728_001232"
                     /pseudo
     CDS             complement(2021..2098)
                     /locus_tag="IW728_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017547333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
     gene            complement(2121..2282)
                     /locus_tag="IW728_001233"
     CDS             complement(2121..2282)
                     /locus_tag="IW728_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1024605.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(2279..2596)
                     /locus_tag="IW728_001234"
     CDS             complement(2279..2596)
                     /locus_tag="IW728_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAQ1024606.1"
                     /translation="MTAEIEIQNAIRRELPKYGHFVYRGNVGKVKTIDGRWFDTGLPK
                     GWPDLFGWTKEGKFFAIEVKNEKGKLRPEQIRFGEFLQKQPVLYGVARSVEEALKIVE
                     ESS"
     gene            complement(2911..5247)
                     /locus_tag="IW728_001235"
     CDS             complement(2911..5247)
                     /locus_tag="IW728_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1024607.1"
                     /translation="MNNYERIPLELRELNQWGIYKRSWNEQRGKWSKKPYDPFTGELG
                     SSTNESKWSDFKTALSAVSTFNADGLAFYFKPPYIGIDLDDIGDDLERYLQGDVENNL
                     VYVFMNSTKTYSEISMSGKGIHIIGKAAIPGERRRKGNVEMYTEGRFFAITGNFFGNN
                     EEINEIPEIQMNFLYKRYLENETVIKQDFSKSNWSDGNDLSVNEIIQTAVNSSTGNRF
                     RLFMDGGWEKIYDSQSEADLAFANDLAFWTAGDFQKMDEIFRMSSLFRDKYDQKRGKT
                     TYGIGLLNKAISENTNHYTGKKTADDYFLSIPGITVDETKPTKFYSYDDTGNAERYLD
                     LFGSFTKYSYVNKCWYFYNGKNWEQDNIGAVRKWVDQTIEIFKNEPVSIPKDATEDEE
                     KAYIEAKEKHLRRSRNNAGKEAMTRELKHQVAILPEEFDSDDMLFNAQNGYLDLSNGI
                     LYEHDISKMFTRISNAEYTDKSDCPRWKLFLEQIFDNNTELIRYIQKAVGYSMSASIR
                     EQVMFILFGNGRNGKSVFLDIISEIMGSYAMGMQASSLMVKQGGSSGHNEDIARLNGA
                     RLVTSSEPNEGVRMDEGLIKQLTGGDKVSASYKGGHMFDYKPKYKIWLATNHKPIIRG
                     NDDGIWRRLPLIPFTVQIPLDKVDKNLKEKLMRELPGIFNWAVEGCLMWQREGLNPPA
                     DIQKATMEYRKEMDIIGAFIDECCETGPDYSIGATDLFKAYDKWARDMNEHPFSQTQF
                     GKKAADRFEKSKSKGKVVYRGIDLKKEFREFNVLVPGL"
     gene            complement(5247..5753)
                     /locus_tag="IW728_001236"
     CDS             complement(5247..5753)
                     /locus_tag="IW728_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAQ1024608.1"
                     /translation="MTAFKVDYNEAQDFGAVPDGDYEVVIFNVTEGASNGGTEFINFD
                     MVIRNDIKQPRQNSHLFHRVWKSKETGKYNRGMIMSLAKSFGLPDGKEYQSFENFLED
                     FAMRPAKVKVKNEQSEYNGKTYDNTNIKKFETTKFPELQHQWKKSNAEKSVNSSPAFD
                     ISDNDLPF"
     gene            complement(5771..7294)
                     /locus_tag="IW728_001237"
     CDS             complement(5771..7294)
                     /locus_tag="IW728_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1024609.1"
                     /translation="MYQLRPYQIKLVQEARKHLSQGKKGVLIQSPPGSGKSVVIAEIV
                     RLATRKGGTVLFLAHRRELLDNIRETLEQNEVDLSKVIILSAVMAKNRLNKLPKLSLI
                     ITDEGHHGKAKTYMDIYNHFKEIPRLGFTATPYRLNGEGFTDIYEEMVEGPSIQWLID
                     HHNLAPYRWYSIPLIDRSKVDFKNMSREAESSARLFESDATIQGDIVENYKKYADGQQ
                     AIVYAPTIQVSKMIVKWFNDNGIYAVHADGKTPTKERDDIMANFKSKKITILSNVDLI
                     SEGFNVPDVGVIILCRPTQSIVLHLQQSMRGMRYRENKISIVLDHVGNGANLGLPTDE
                     FEWSLSARKKKSNGSSSEAPRMTCSTCGQQFLLKSLLRIENKPHCPFCLQEIVIKEKE
                     NSVTFDEAVQMVELNAENAKLARLSRKKFSKKQSLELNYAIAKAKVSFEGKGNPLFKM
                     FGSLTAYQGQHYSIDQLEELSLIKDVSMESILRAYKWALEKLNSKQEEPEWVKNTFY"
     gene            complement(7266..7976)
                     /locus_tag="IW728_001238"
     CDS             complement(7266..7976)
                     /locus_tag="IW728_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010721532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1024610.1"
                     /translation="MQIKKATDLKRNKDWRIMVYAKPGTGKTSLVQYLKGKTLVLDMD
                     DSSKVLAGLPNVDVISFDRVHPDEFITEFLKEASQLIKDYDNLVIDNISSFEKDWFVE
                     RGRSSKNGISNEIQDYSQWTNYFARIMTTIYLLKDINILVTAWENQREITTERGQVFN
                     QYAPQIRDSVRDNLMGLTDIVGRMIINPDTGNRGIILEGNDAVYAKNRLDERKATPAE
                     ELFFIGGEPNVSTPTVSD"
     gene            complement(8076..8507)
                     /locus_tag="IW728_001239"
     CDS             complement(8076..8507)
                     /locus_tag="IW728_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024611.1"
                     /translation="MKNKLAKTTVIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREIEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            complement(8909..9388)
                     /locus_tag="IW728_001240"
     CDS             complement(8909..9388)
                     /locus_tag="IW728_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAQ1024612.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            complement(9321..9638)
                     /locus_tag="IW728_001241"
     CDS             complement(9321..9638)
                     /locus_tag="IW728_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024613.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            9806..10264
                     /locus_tag="IW728_001242"
     CDS             9806..10264
                     /locus_tag="IW728_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024614.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            complement(10355..10537)
                     /locus_tag="IW728_001243"
     CDS             complement(10355..10537)
                     /locus_tag="IW728_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024615.1"
                     /translation="MDKLNTTIVFSAPIIIYLLSVWGNIPALIGTIVYMVWIFIGLDE
                     AEYRAKKASREGLTNE"
     gene            complement(10586..10846)
                     /locus_tag="IW728_001244"
     CDS             complement(10586..10846)
                     /locus_tag="IW728_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1024616.1"
                     /translation="MEVILTPENEAALRSYIHEIITDEIAKARRDASVDKRVLKQIEI
                     AKYFGVSTATIRKWEDKGLPFGRIGDQKFYDKEKCRAWVLAQ"
     gene            complement(10860..11051)
                     /locus_tag="IW728_001245"
     CDS             complement(10860..11051)
                     /locus_tag="IW728_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024617.1"
                     /translation="MSNIDNGREAIKEFMKANNISEYDLATAYGRSRTWIQRVLSGKD
                     KGPAVNAFILEVIRDHKIR"
     gene            11343..11666
                     /locus_tag="IW728_001246"
     CDS             11343..11666
                     /locus_tag="IW728_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1024618.1"
                     /translation="MTVFERIKFLAKKHSKTMKQVTIDLGYSENYFYSLKSGKQPSAE
                     KLTEIANYFNVSVDYLLGREKKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKA
                     LEENE"
     gene            11684..12106
                     /locus_tag="IW728_001247"
     CDS             11684..12106
                     /locus_tag="IW728_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1024619.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSALYDNSTTFHSKFETEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            12188..12823
                     /locus_tag="IW728_001248"
     CDS             12188..12823
                     /locus_tag="IW728_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ1024620.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            12931..14079
                     /locus_tag="IW728_001249"
     CDS             12931..14079
                     /locus_tag="IW728_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1024621.1"
                     /translation="MATFEQYKKKNGAKLWKFQTYLGVDPLTGKQVRTTRRGFKTKKE
                     AQLALTKLQLEYESNGLNKSKELTFQEVYDLWIVNYEQTVKESSFVKTKEQFANHILP
                     AFGALKINKISIDIAQKFANEKVKRFVLYREFINNASRICDYAIKLGYLQDNPFKKIT
                     VPKRKVSVHEENTLNFFNKEELEIFLKSVEKKKDIRMYSFFRTLAFTGMRVGELLALT
                     WKDIDFNDNYIKINKTLARGKNRRLYVEQPKTKNSKRDIPVDDETMNILKKWRLEQRK
                     WLLTLGINTLSKNQLVFSNQKNEYLQLSKPRKWLEVIIKQNNLKRITIHGLRHTHASL
                     LLEAGANIKDVQERLGHSSIQITMDLYIHITDKRKEKTAAQFAKYIGI"
     gene            complement(14186..14273)
                     /locus_tag="IW728_001250"
     tRNA            complement(14186..14273)
                     /locus_tag="IW728_001250"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14239..14241),aa:Ser,seq:cga)
     gene            complement(14418..14984)
                     /locus_tag="IW728_001251"
     CDS             complement(14418..14984)
                     /locus_tag="IW728_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1024622.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            15240..>15435
                     /locus_tag="IW728_001252"
     CDS             15240..>15435
                     /locus_tag="IW728_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1024623.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYF"
BASE COUNT         4528 a         3062 c         2396 g         5449 t
ORIGIN      
        1 cttttcgcta accacttgtc gcattccatc gtgttttcaa tgcgaatgat cgctgagtga
       61 ttatagagat gctctacata tccacgaaac ggatagatga acccttctgc ttcgcagcga
      121 accatgtcac cgactttgaa ttttggtttc ttacgtgttt tagggttctt tgtcggcata
      181 tctagcatta aacctccgat accgtgactg ctagagtaga atccgtcttt tagtttcatt
      241 actctacctc ctcgtgatcg atagtgacca gttcatatac ttgcgctaat ccaccaagtc
      301 gtcgtgaaac ttggttagct tcttcgagat tatcaaacca tcttgcactt tggagtatgt
      361 ctacaatgga taatgtgtta gcccctatag aacgttcatc acttctgtaa aattgatttc
      421 caaactttac tacataaatc ttcattctgt atcctcccat ttaatatcta gtatcgaaat
      481 tccaaactta cgaatagcct cactcgcatc agcaacacac tgacttgcca ctttgtatgc
      541 ttcttctata gaaattccgt attctttttc aaacttcgcc ttcagtgcat taagttcttg
      601 ttttcttagt tttgttactc tgcggtgtct gttgttcatt gtcaatcaac tccctaatct
      661 gaaagtgtcg tttatacttg atcgaaattc tttcaagtgg ttctctacca cagaatcagt
      721 cacgttaaaa cgatcaatta atacaggagc tgccatatct ttcaaataac tttgtctgat
      781 aactatttca gtaccatcag aaagtttgaa tttaactttg cgaccattaa taattgactg
      841 aacgtctgct tcgcttagtg ctatttcgta tttcattctg cttcctcctg ttttattttc
      901 accatttctt tgttgaaaac ccgcaacgtc tatactgttt aattcgtttc ttaggtcttt
      961 ctagatcaac cagaataaat ctgtcggata tatgataatc ttctattaca gccgtataca
     1021 ttggattcga aaccgagtac catgatcttt ttttatcaac ctcatccgca tcatctcttg
     1081 taatttctat catgttagca gacattagat agctctcttc ttctgcctcg aatgggcagg
     1141 aatattcatc ccagcttcga ggaaacctgg ccaaatctct ttttaaataa acttgaacat
     1201 tcattccgct tcctcctcaa tcgtccaagc atttgtaaat ttctctaagc ttattagttc
     1261 atcttcattt aaatatccgt aaccatataa ggattctaaa aggtcttctt ttgaatagcc
     1321 tctaaaatca agtctgagac cgcttaggat gtatacaaag gttttatttc cataaaactt
     1381 gattgtgtat gctttcattt ctattcctct tgttctaatc cttctatcac tggcacagca
     1441 aatggccaat atctttcatc aattgctttt atttctgatt ccgtaaattt catatcatca
     1501 aaatcattgt tgtctgcaat tgttatattt cctcgatcat ctttcattag atacgtctct
     1561 aaatccaaat gattaaaata cacaactggt aacttcacat aatacaacgg ttctttctca
     1621 acctcgtaac catacagcaa cgcatttatt gctttttctt tattaagata aaggctatct
     1681 tcaacaagcc aatctagtaa ctctcttgtt gtggaagaat tttcgctttt accaagttca
     1741 tagtccacgc cataacccca acctgcacgt gcaataagag aaatggcata aaacttgtca
     1801 cgttctgcta taactctttt tgcccaatca tcaaaaaaac ttaggcataa ctggtttctg
     1861 cgattcgtct agttttaaca ccgtattaac catatgaacg acttgagaat aagccatcac
     1921 catgccctca tagtaatttt tgctttctgg atgcgtcagg gctttgtgct gtttttctag
     1981 tgaaattctc tttagttcat taatcaattc ctgtttattt ttattcattg ctgtcctcca
     2041 agtattcgtc taatatctct ttatatttct ctacaaattt gaaacgatct tgatgaacat
     2101 tgtcgctttc ttatccacgc tcattcctcc aatctacgaa tttcccttct taagttctct
     2161 atgtgcaaat cgattgcctt tctcgccgtt tcattgacca tcactgcctt tgttcgttct
     2221 agatcgtcaa tttcacgctg aatgtttcga atacgcattt gaatcacttc ttctgttgtc
     2281 atgatgattc ctccacaatt ttcaatgctt cttccactga tcgtgcaaca ccatatagga
     2341 ctggttgctt ttgcaaaaat tcgccaaacc gtatctgctc tggacgtagt tttccttttt
     2401 cattcttcac ttcaatggcg aaaaattttc cctctttagt ccatccaaat aaatctggcc
     2461 atcctttggg caatccagta tcgaaccatc tgccatcaat cgtcttcact ttgcccacat
     2521 tacctctata aacaaaatga ccatattttg gtaattctct tcgaatggca ttttgtattt
     2581 caatttctgc tgtcataagc gcctcctgct cgggtagata gtatatatac atgggtagac
     2641 agtttataat cgctctatcc cttgtggctc taagtattaa ccagtttggg tagacagtag
     2701 acagttattt ctaggtcgct tctgtatttt tatatatttc cttattttta ttattttatt
     2761 tactatatat ataaactatc cctactatct acctaacagt aatgagcctt acagcctcaa
     2821 aggttttatc gggtagacag tttgtaaaaa actatctact tactatctac ccaactgtct
     2881 acctacacgc atttgtgaaa cttttttgtt tcacaatccc ggaactaata cattaaattc
     2941 tctaaactct ttttttaaat caatccctcg ataaacaact tttccttttg attttgattt
     3001 ctcaaacctg tctgcagctt tcttaccaaa ctgggtctga ctgaatggat gttcattcat
     3061 atctcttgcc catttatcgt atgctttaaa tagatccgtt gccccgattg aataatcagg
     3121 tcctgtttcg caacattcat ctataaaagc acctataata tccatttctt ttcgatattc
     3181 cattgtagct ttctgaatat cagctggtgg atttaatcct tctctttgcc acatcaaaca
     3241 tccttctact gcccaattga atattcctgg caattcacgc attagttttt cttttaagtt
     3301 tttatccact ttatccaatg gaatttgtac agtgaaagga attaatggca atcttcgcca
     3361 gataccatca tcatttcctc tgatgattgg cttatgatta gttgcaagcc aaatcttata
     3421 tttaggttta tagtcaaaca tgtggcctcc cttataggat gctgacactt tatctccacc
     3481 agttagttgt ttgattaaac cttcatccat tcttacgcct tcatttggtt ccgaggacgt
     3541 tactagacgt gcgccattta atcgcgcaat atcttcgtta tggccgctgc taccaccttg
     3601 tttaaccatc aagctcgatg cctgcattcc catggcataa cttcccatta tttcagaaat
     3661 gatatctaag aaaacagact taccatttct tccattaccg aaaaggatga acatgacttg
     3721 ttctcttatc gatgcactca tagaataccc aacggctttt tgaatatagc gaatcaattc
     3781 agtgttattg tcaaatatct gttccaaaaa cagcttccaa cgtggacaat cacttttatc
     3841 cgtatactca gcgttagaaa ttcgagtgaa cattttagaa atatcgtgtt cgtataaaat
     3901 accattagaa agatctaaat aaccgttttg agcgttgaac agcatatcat cactatcaaa
     3961 ttcttcagga agtatcgcta cttggtgttt caactcacgt gtcatagctt cttttccagc
     4021 attatttcta gaccttctta aatgtttctc ttttgcttca atataggctt tttcttcatc
     4081 ttcagtcgca tctttaggta tcgaaacagg ttcgtttttg aatatctcga tagtctgatc
     4141 tacccatttt cgaacggcac cgatattgtc ttgttcccag tttttaccgt tataaaaata
     4201 ccaacattta tttacgtagc tgtattttgt aaatgatcca aacaaatcaa gatatctttc
     4261 tgcatttcct gtatcatcat agctataaaa cttagtcggt ttagtttcgt ccacagtgat
     4321 acctgggatg gaaagaaaat aatcatcagc tgtttttttg ccagtataat gatttgtatt
     4381 ctcagatatg gctttattta aaagccctat tccataggtt gtctttccgc gtttctgatc
     4441 atatttgtct cgaaacaacg aagacattcg gaatatttca tccatctttt gaaaatctcc
     4501 agcagtccaa aaagctaaat catttgcaaa tgctaaatct gcttcagatt ggctatcata
     4561 tattttttcc catccgccat ccatgaataa tctaaaacga ttacctgtgg aagaattcac
     4621 cgctgtttgt atgatctcgt tgacagataa gtcatttcca tctgaccaat tacttttaga
     4681 aaaatcttgt tttattactg tttcattttc taaatatcgc ttgtataaga aattcatttg
     4741 gatttcaggt atctcattaa tttcttcatt attaccaaag aagttacctg taatagcaaa
     4801 gaacctacct tcggtataca tttcgacatt cccttttcta cgtctttctc cagggattgc
     4861 tgctttacct ataatatgga ttcccttgcc actcattgat atttctgaat atgtttttgt
     4921 agaattcata aaaacataaa ctaagttatt ttctacatcg ccttgaagat acctttctaa
     4981 atcatcgcct atatcatcta aatcaatacc tatataaggt ggtttaaaat aaaaagctag
     5041 gccatcggca ttaaaagttg aaacagctga gagagcggtc ttgaaatcag accacttgct
     5101 ctcatttgta ctgctcccta attctccagt aaacggatca taaggtttct tactccattt
     5161 ccctcgttgt tcgttccatg agcgtttata aatcccccac tgatttaact cacgcaactc
     5221 taagggaata cgctcgtaat tgttcattag aatggaagat cattatctga aatatcaaat
     5281 gctggtgagg aatttacaga tttttcagcg ttgcttttct tccattgatg ttggagttct
     5341 ggaaacttag tggtttcgaa ttttttaata tttgtattgt catacgtttt cccgttgtac
     5401 tccgattgtt cgttctttac ttttactttc gctggtctca tagcaaaatc ttcaaggaaa
     5461 ttttcgaatg attgatattc ctttccatca ggaagcccaa atgatttcgc tagtgacatg
     5521 atcatcccac gattatattt gccagtttct ttacttttcc aaactctatg gaaaagatga
     5581 ctgttttgtc gtggttgttt aatatcattt ctgatgacca tatcaaaatt aataaactct
     5641 gtgccgccat ttgaggcacc ttccgtaacg ttgaaaataa ctacctcata atctccgtct
     5701 ggtactgctc caaaatcttg tgcttcattg taatctactt taaatgctgt cataattaat
     5761 tacctcttcc ttaataaaat gtatttttta cccattcggg ttcttcttgt tttgaattga
     5821 gtttttccaa agcccattta taagctctta aaattgattc cattgataca tctttgatca
     5881 acgataattc ttctaattga tcgatggagt aatgttgtcc ttgataagca gtgagtgagc
     5941 caaacatttt aaaaagcgga ttccctttac cttcaaaact tacctttgct tttgcaatcg
     6001 cataatttaa ttctaaagat tgtttttttg aaaatttctt tcttgaaagt cgcgctagct
     6061 tggcattttc tgcattcaat tccaccattt gaaccgcttc atcaaaagta acggaatttt
     6121 ctttttcttt aattactatt tcttgtaagc agaacgggca atggggttta ttttctatcc
     6181 ttaataaact cttcaaaaga aattgctgtc cacatgttga gcaagtcatt ctaggcgctt
     6241 cgctgctact tccattactc ttctttttcc tcgcgcttaa cgaccattca aattcatcag
     6301 taggtaaacc taagttagct ccgttcccaa catgatctaa cacaatcgaa atcttatttt
     6361 ctcgatatct catgcctcgc atagactgtt gtaaatgtaa gacgattgat tgcgtaggcc
     6421 tgcaaaggat aatcactcca acatctggta cattaaatcc ttcgctgatt aaatcgacat
     6481 ttgataatat agtgattttt ttcgatttga aatttgccat aatatcatca cgttctttgg
     6541 taggtgtttt tccatctgca tgaaccgcat atattccatt gtcgttaaac catttaacaa
     6601 tcatcttgct tacctgaatc gttggagcat aaacaattgc ttgttgacca tccgcatatt
     6661 ttttataatt ttcaacaatg tcgccttgaa ttgtggcatc tgactcgaat aatcgagcag
     6721 aactttcagc ttcacgtgac atatttttaa aatctacttt agaacgatca atcaaaggaa
     6781 tagagtacca acgataaggc gctagattgt gatgatcaat caaccactgt atggatggtc
     6841 cttctaccat ttcttcgtat atatctgtaa atccttctcc gttgagtctg tatggcgtag
     6901 cagtaaaacc taacctagga atttctttaa aatggttata aatatccata taagttttcg
     6961 ctttaccatg atgcccttca tccgtaatta tcagagacag ctttggcaat ttatttaacc
     7021 tgttcttagc cattacagct gacaatatta tgacttttga taaatctact tcattttgtt
     7081 caagagtttc tcgaatgtta tctagtaact ctctcctgtg ggcaagaaat agtactgtgc
     7141 caccttttct tgttgctaat cgaacaattt ctgcaattac aactgatttg ccacttcctg
     7201 gtggtgattg gatcaatact ccctttttcc cttgagatag atgttttctg gcttcttgaa
     7261 ctagcttaat ctgatacggt cggagttgat acattcggtt caccgcctat gaagaacaat
     7321 tcttctgcag gtgtagcttt ccgttcatct agtctgttct tagcataaac agcatcattg
     7381 ccttctaaga taatccctcg attgccagta tctggattga taatcattcg cccaacgata
     7441 tcagtcagac ccataagatt atctctgaca ctatctctga tttgtggtgc atactgatta
     7501 aatacttgtc cgcgttcagt agttatttca cgctgatttt cccatgcagt tactaaaata
     7561 ttgatgtctt ttaatagata aatggtagtc ataattcgag caaaatagtt cgtccattgt
     7621 gagtaatctt gaatttcatt actaattcca tttttagaag atcttccacg ttctacaaac
     7681 cagtcttttt caaaacttga aatattatcg ataacaagat tgtcataatc cttgataagt
     7741 tgagatgcct ctttcaaaaa ttccgtgata aattcatccg gatgaacacg atcaaatgag
     7801 atcacatcta cgttcggcag acccgctaaa actttcgagg agtcgtccat atcaagcacc
     7861 aatgtcttac ctttcagata ttggaccaac gatgtttttc ctgttcctgg tttcgcatac
     7921 accatgatgc gccaatcttt gtttcttttt agatccgtcg ccttcttaat ctgcatcaaa
     7981 attctccttt ctattggtta aaaaagtttt tcgattgtcg gtatgctgca ttgtcgatcc
     8041 actatcttct tcaactgata tagtgattct tttaatcatt tacccaactc ccctactttt
     8101 tgatctgtat actgccgtaa ttcgctcaca cgctgttcta actgctcctt gtcgttttgc
     8161 actgtggaca attgttggcg caagctatcg gcttcctgtt gctttgtagc gatctcctgt
     8221 tgcttttgct cgatttctcg ttgcttggct tcaatttcct tttgcttgtc cgatttgatt
     8281 tgctcgattt ctgctttcag ctgctcctgt gtgcgagtgt tgttggatag ctgtgattcg
     8341 agttctgaca cacgttgcga tttcgtttga ccgtattgta ggactgtgtt gaaatttgct
     8401 ttgatcgtgt ccaaatcttg aaacgcgttg cttgctgcgt agccgataac gccgctacct
     8461 agtgctagtc caatgattac tgttgttttt gctagtttgt ttttcaatgt tgtttctcct
     8521 ttatatgttt atctgccgat ttaatcggtt ggcgtatagc agttcctggt acctttttaa
     8581 ttcgtgaatc aattgagcgc gttggtgatc gctcatttgg tttttcttgt cgtttagttc
     8641 gtcgtgtagt gatctaatct tttcttttgc taggtcgtgg atggatagtt cctgttgtag
     8701 tgtgtaggtc ataggctatt tacccagata cacttctgac atgccgagta aatcagcaac
     8761 gttatatagt agttctcgaa ccatttcttg gacatcttct gttgttgctg gcttaccatc
     8821 aacttttatt ccctttgatt ctgcaatttc gatagcagct ttcaaagcgt cttttacgtt
     8881 ttcgtgttcc attatttctc ctccttcatc atcttatcct caaacctttt gtttggacga
     8941 gttcagcacc aggaatatct ccgtgcttca gttcctcctt caattgcttt ttatccaatt
     9001 ttggaggcac aggggtaaag aatccttttg gaattaagtt ctcattgatt acattgaccg
     9061 atactggatt attttgaatt ccaatgttga ataattcacc tttaatcttc gttttaccag
     9121 tcttttccat ttcctcttgt aaatagtgct tgatactctt agcattgttc aaaagcgatg
     9181 tcttacgttc ctgtagacgt ttaatttcac tatcgattaa ctcagctttc ccttctactt
     9241 ctttaactat ttttgctaag ttttctgcct tatactcgat cgcttcatta atcgaatcga
     9301 gagtatcgcg aagaatttct tcatccaatt gttctgccag ttccaggact ttgatatatg
     9361 actcgctgag ttggtaaaga gttgccattc ttttgtgcct cctttaataa ttttgcaatt
     9421 tgttcaaaag ctaggattgc ctcatctaaa tccaattcca cagaatcatc aatttgttcg
     9481 aaagcaaggt ttgtttcttc aatatcactt gcttgataga taccaatttt tccattgtca
     9541 taaagatcaa atactaaaat tcctgacgca tctatattgc gcagtttgta ttcgtctttt
     9601 aaaaagatgc ggtctagtgt atccgttgca attagcattt acggttcatt cctttctgtg
     9661 gtaaaatata aaaagataat ttatttcctc gacacgatca tgcttgccgg cgttcgtgtc
     9721 tttttttatt tttcaaaatt actaattctt gcattatttc ttctctcctt tttgatataa
     9781 tttgagtaaa aaggtggtga attacttgat aaaaattttg agcgattacc aagtcgctat
     9841 tactttaatc atttctattt caggattttt tttatccctc tataacttat ttaaagataa
     9901 aagaaaaatt acgctctcct attttttagt taagcatgat agaagtaatc gaatgattct
     9961 tactggcgta attgcaaacc catcaaaaat gccaaactca attatcgaat gtacgtatct
    10021 ttacaacggt aagaaaatta attgttcaca ttatcacgcc aatggttttg atatgggaag
    10081 cattcataaa agtagccttt tatctcctat accgttggca caagcaatct ctcctggcta
    10141 ctcagtcgct ttttcagaag ttttagatat gaaagatata ttacctggtg aaaaacttgt
    10201 aatggtcata agaacagcta attatcaaaa aaaatttaag ttaaagctaa aagactcatt
    10261 ttaattgaaa aatttttctt taataatcaa atacaaatta attattattg atccaatccc
    10321 acaaaacata attaacagaa atacaatatc gtccttactc attagtcagt ccctcccgac
    10381 tggctttttt cgctctgtac tcagcttcat caagcccaat gaaaatccaa accatgtaaa
    10441 cgatcgtgcc gattaatgct gggatatttc cccagacact cagcagataa ataatgattg
    10501 gtgcactgaa tacgattgtt gtatttagtt tgtccataaa tcccccttct ttactgtcat
    10561 tatttccgaa cacttacccg atattttatt gtgctagaac ccatgctcta catttttctt
    10621 tgtcgtaaaa tttttgatcg cctatacgtc cgaatggaag tcctttatct tcccacttac
    10681 gaatagttgc agttgatact ccgaagtatt tcgctatctc tatttgcttt aatacacgtt
    10741 tatcaactga ggcatctctt cttgcttttg caatttcatc agttatgatt tcatgaatgt
    10801 agctacgaag tgctgcctca ttttcaggcg ttaagattac ttccataatc ctgaacctcc
    10861 tatcgaattt tatgatcgcg aataacttcc agaataaagg cgttaacagc tggaccttta
    10921 tcttttccac ttaaaacacg ctgaatccaa gttctcgatc taccatatgc agtggccaaa
    10981 tcgtattctg aaatattatt tgctttcata aactctttga tggcttcccg cccattatcg
    11041 atattactca cttcacacac ttcctttctt tttatttaga aagaaaattg gatagaaaag
    11101 tatattttta gttgactgca aaccatacta tagtgtagta tatagccata gttaaataag
    11161 catataacaa gcctttttat cgcactcggt cgccaaactt aatgctataa ggtgtgtttt
    11221 tactttgctt tttttctatc caattaactt acaaaaccaa tatacactat agtgttgttt
    11281 aggtcaacac aaaactacac ttttttattg gttttttgta aagaaaaaag gagaatgctg
    11341 gtatgacagt atttgagcgg ataaaatttc ttgcaaaaaa acattcaaaa acaatgaaac
    11401 aagtaacaat tgatttagga tacagtgaaa actatttcta tagtttaaaa agtggaaaac
    11461 aaccatccgc tgaaaagctt actgaaattg ctaattattt caatgtctct gtagactatc
    11521 tactaggaag agagaaaaaa gagaccccta aacatgtgga tttatcagaa gacgatactg
    11581 tattctcttt tgacggaaaa gaaatctcta aggagacaat gcgtaaagcg attgcaattg
    11641 ctaaagcttt agaagaaaat gaatagttgg agtgatgggt tgtatgtatt taaagttgaa
    11701 agaaatgctg agtgagtata atttaaagtt aatctatatg gaaatggaag aaccaggttt
    11761 ttattatcca aaaccaagaa tagtattttt gaatgaaaaa ctacacgaag acagttctga
    11821 agcttttcat ttagcccacg agctcggtca tttcattgct tcacattttg aatattcagc
    11881 attgtacgat aactctacaa cttttcattc aaaattcgaa actgaagctg atagaatcgc
    11941 tattatgatt ctacttaata tctttattga gaatgaatta acagatgaat ctcagttcaa
    12001 attggaaaat tttatggagt tctattctat caataataag ttaagaacag aatgttttaa
    12061 tgtttgccag tcttatttca agaaaaaata ctcttatgca cagtaaaaaa gcccgtgctg
    12121 caacacggac tcatacctca tttctgagat cacaaatata ttataacaag aaatgaggaa
    12181 tatttaaatg gcaaaaaaag ttatgggtca agatgggaaa atgtataagg ttaaaaaacc
    12241 gttttataaa cgggtatggt tttgggtatt aatagtactt ttgttttttg gtattggtgg
    12301 tgctctaagt ggcggtgaaa acaaagattc agctactgca acatcaacaa gtactaataa
    12361 agaaactaca aaagaaacta caacaaaaga agaaaagact tataaaatcg gtgatgacgt
    12421 tgctgtaggg aaaatggaat acaaagtaaa ctccgttgaa gtagtaaaac aagttggccc
    12481 atctgtactt cctactaacg caaaagacac tttcttagta gttgacttat ctgtaaaaaa
    12541 tgcaggtgat aaggctgtga ctgttgatag ctcattcttc aaattaaaag cagatggaaa
    12601 aactttcgaa gctgattcgg ctgcatctat gtcggcaaat caagatgaaa atggtaatat
    12661 cactaactct ttctttatgg aaagcttaaa tcctgatatg caacaaactg gtaaaatagt
    12721 ttttgatatc tctgaagccc aagcaaatgc acaaaataat gtattgcaag cacaaacagg
    12781 ttactttgga acagagacag tttcaattgc tcttcataac taattagtat aaaaaaaaca
    12841 cacccaccgt ccaaagtaag atcgtataac taaggaggtg atgccagcta ttttagtccg
    12901 aacacttacc cgagcgaaag gacgaaaaaa atggcaacat tcgaacaata caaaaagaaa
    12961 aacggtgcaa aattgtggaa gtttcaaact tatttgggag tagatccctt gactggcaaa
    13021 caagtgagaa ctacacgaag aggttttaaa acaaaaaaag aagctcaatt agcgctgacc
    13081 aaattacaat tggaatacga aagtaatggt ctaaataagt ctaaagagtt aacttttcaa
    13141 gaagtatacg atctatggat tgtaaattat gagcagacag taaaagaaag ttctttcgtt
    13201 aaaacaaaag aacagtttgc gaatcatata ttaccagcat ttggtgctct taaaatcaac
    13261 aaaatatcga ttgatatagc tcaaaagttc gctaatgaaa aggtaaaaag atttgtgttg
    13321 tatagagaat tcatcaataa tgcttcgcgt atatgtgatt atgctattaa attaggatat
    13381 ctacaagata atccttttaa aaaaatcaca gttccaaaaa gaaaggtctc tgttcatgaa
    13441 gaaaatactt taaacttttt taataaagaa gaactagaaa tctttttgaa atcagtagaa
    13501 aagaaaaaag atattcgtat gtattctttt tttcggacac tagccttcac agggatgcgc
    13561 gtaggcgagc tcttagctct cacatggaaa gacattgatt ttaacgataa ttatatcaag
    13621 ataaataaaa ctctcgccag aggaaaaaat agacgccttt atgtagagca acctaaaacc
    13681 aaaaattcta agcgagatat accagtcgat gatgaaacta tgaacatctt gaagaaatgg
    13741 cgattagaac aaagaaaatg gttgttaaca ttgggaatta atacgttaag caaaaatcaa
    13801 ctggtatttt ctaaccagaa aaacgaatat ctccaattat ctaagcctcg taaatggtta
    13861 gaagtgatta tcaaacaaaa taatcttaaa cgtattacta ttcatggtct tagacataca
    13921 catgctagtt tacttttaga agctggtgca aatattaagg acgtacaaga acgtttaggc
    13981 cactcgtcta ttcaaatcac tatggattta tatatccaca ttacagacaa acgaaaagaa
    14041 aaaacagcag cgcaattcgc aaaatatatc ggtatttaag cagaacgtat tcaaagacgt
    14101 attcaaacaa aaaaacagag gcgaaaacat tgcaagcaaa tgcctgtata tcaatgtttt
    14161 cgcctctatt ataaaatgtt atataaggaa ggtacaggat ttgaacctgc gcgccggtat
    14221 tagccggttc gccggatttc gagtccggtg cattaccact ctgccaacct tccgtaagta
    14281 ctatttttaa acttttttca cgaaatgtca agcctatttc taatagataa ataacagaaa
    14341 tcgctttttt tagcaaaaaa gtccattgac agtttgcagc caacggactt accgatcaaa
    14401 ttcatactga tagaacacta tctgattatt tcaggattat ctaatgctaa aatcgagtca
    14461 attgtccgtt tgatcagtgc aatgatttgt tcttctgttg gatgcatgat gtttgatgac
    14521 atcaatgacg ctaagccagt cactgctacc catgtaccag tatgcagagc atcgacatat
    14581 tcatccgata attgattgta tctttcatct tctttaacta tttcacggaa ataattgtat
    14641 gaaaaatcat gcattttctt tccgtcccca tgttcatcaa tgtacaatgc catgtaaaga
    14701 ctatgatgct tttttgcaaa gtagatatag ttcaaaccga gatcaatgat tttatcacca
    14761 gtacgttcaa tagggaaaac atcttctttt agttgttcaa agatagaatc cagcaacgta
    14821 ttttttaaat cttccatgtt tttgaattcc agataaatcg gctgtgtcga aatccccatc
    14881 tttttagcaa tatttctcgc agtaaatcct ttaaaccctt cctttgcaac aacatcataa
    14941 gcagctttta gaatttgatc cttggtatag acttttcttc tcattaaaac tcccccactt
    15001 ctttactgca gtatatgttg ttttattgta ccctattttt agcatttttt gcaaacattt
    15061 tactcaaaac gttatatttt aaattttttt tcagtcttct ttaacttttt tttgcaaaat
    15121 taaaaaaatt tggttaaatt caatgggttc ccttacattt ttagttttga cctaaatgat
    15181 agttgtccaa atctttcatt tcaagttata gttaaagaaa atgaaaaaga aggtgtgtaa
    15241 tggaaaacga aactgacgtt atttatattc atcctcaaaa acgaatcgtt tcacagaaaa
    15301 gaaagtattt ttatcttggt tttacaggtg ttttttttct ttttattggc ttgctttcta
    15361 atactcctac tgataattgg agtggattgc taactattct tacctcgcca agcaatcttt
    15421 tgacagatta ttttg
//
LOCUS       DACEMO010000053        15369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEMO010000053 DACEMO010000000
VERSION     DACEMO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.054"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(12..482)
                     /locus_tag="IW728_001253"
     CDS             complement(12..482)
                     /locus_tag="IW728_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1024624.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="IW728_001254"
     CDS             complement(641..1099)
                     /locus_tag="IW728_001254"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1024625.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2414)
                     /locus_tag="IW728_001255"
                     /pseudo
     CDS             complement(1270..2414)
                     /locus_tag="IW728_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            complement(2427..3344)
                     /locus_tag="IW728_001256"
     CDS             complement(2427..3344)
                     /locus_tag="IW728_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1024626.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3772..4335
                     /gene="efp"
                     /locus_tag="IW728_001257"
     CDS             3772..4335
                     /gene="efp"
                     /locus_tag="IW728_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1024627.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4821..6080
                     /gene="lysA"
                     /locus_tag="IW728_001258"
     CDS             4821..6080
                     /gene="lysA"
                     /locus_tag="IW728_001258"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1024628.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6067..6867
                     /gene="dapF"
                     /locus_tag="IW728_001259"
     CDS             6067..6867
                     /gene="dapF"
                     /locus_tag="IW728_001259"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1024629.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6870..7751
                     /locus_tag="IW728_001260"
     CDS             6870..7751
                     /locus_tag="IW728_001260"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1024630.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7748..8464
                     /locus_tag="IW728_001261"
     CDS             7748..8464
                     /locus_tag="IW728_001261"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1024631.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8470..9165
                     /gene="dapD"
                     /locus_tag="IW728_001262"
     CDS             8470..9165
                     /gene="dapD"
                     /locus_tag="IW728_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1024632.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9181..10182
                     /locus_tag="IW728_001263"
     CDS             9181..10182
                     /locus_tag="IW728_001263"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1024633.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINENRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10179..11540
                     /locus_tag="IW728_001264"
     CDS             10179..11540
                     /locus_tag="IW728_001264"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1024634.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11500..12651
                     /locus_tag="IW728_001265"
     CDS             11500..12651
                     /locus_tag="IW728_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1024635.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12626..13795
                     /locus_tag="IW728_001266"
     CDS             12626..13795
                     /locus_tag="IW728_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1024636.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13970..15349
                     /locus_tag="IW728_001267"
     CDS             13970..15349
                     /locus_tag="IW728_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1024637.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         5013 a         2716 c         3275 g         4365 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatctttt ggttagaagt cactaaacca
     1801 gctactacat cgctgtgtcc gcctagatat ttggtaccgc tatggacaac gatatcagaa
     1861 ccgagatcta aaggtctttg aaaataagga gtagcaaatg tattgtcagc aataactatc
     1921 aatccatgtt gatgagccag agtggcactt ttttctaaat cagtaatctt tagaagcgga
     1981 ttgctaggtg tttcaagata aagtgctttt gtattgggtt tgatggattg ttctatttta
     2041 tcaagattac tcgtatctat gatcgtgtac tcgagaccat ttttagtaag aactttgtca
     2101 aatagacgga atgttcctcc atagacatca tctcctaata agatatgatc gccagcttga
     2161 aaaagagaaa aaacggcatg aatcccagaa agaccagaag aaaaagcgaa tcctcgaaca
     2221 ccgccttcca aatcagcaat cagttcttcc aatgcaaaac gagtaggatt accagatctt
     2281 gaatattcat attgtttagg ctggccgacg ccatcttgcc gataagtgga tgtttgataa
     2341 ataggtacac tgacagctcc tgttgtggga tcttcactga tgcctccgtg gatcaatttt
     2401 gtttgaatgt gcatgtttct ttcctcctat aagtaaatgt tttttgataa atagcggtct
     2461 gctgcgtctg gaaaaatcgt aacgacacga tgaccaggtg gtaaacgacg aatttctctt
     2521 aatgctgctg caaaagccgc accactcgaa cttccaacta atagtccttg ttctcgtgct
     2581 aactgcctag tatagttaaa cccttctaca tcagagattg tctcgatctg attgattgaa
     2641 aggggagaaa gaaacggcgg aatgaattct acaccgatac cctcaatttc gtgaggggct
     2701 ggatcaccac catttaacac agagccctct ggttctacac cgattattcg aatgtcggga
     2761 tatttttctt tcaaaaaggt agaagttcca gcaaaggttc caccactccc aattcctgct
     2821 acaaaactat ggatattttc tttgatttca tgaaagattt ctggccctaa cgtatgatag
     2881 taagcagcag gattatcttt attttcaaat tgaagaggca gatagctgtt tgatatctcc
     2941 tctgcaagtt ttttactttt gctgatggca ccgatgattc cttgctcact aggagaatgg
     3001 atgatctttg ctcccaatgc tttcatcaat tgttgttttt ctaagctaaa cttctctgga
     3061 acaacaaaaa ccgttcttaa tttgtatgtg agtgcggcca gagcgatccc aattcctgta
     3121 tttccggcag ttggttcgat gatcgtcgtt ttttcattga tttttccttg ggcaaatcct
     3181 tctttgatca aataactgcc caatctatct ttgatacttc ctccaggatt taaatattct
     3241 agttttgcat aaatagcaga acctttaggc atagaatagt gctgattgtc aaagcgataa
     3301 agcggtgtgg aaccaatagc ttctgtaatt gtcgagataa tcatgtgggc ctcctaaaaa
     3361 aagataataa aaaaaggagc tgactattaa atcagctcct tgaaagacat agagaaaaat
     3421 tgagtagata aaaagataag ttacctggta ctcattctat gaagggcaat taatagacat
     3481 gacaaatagt atgctgcttt tatgcaccat acaacagcaa cgattcaatt gtttttggtt
     3541 gctgactgtc atgtgtatcc ctccaagtaa aatttttatt aacgatagca tgaaaaaaaa
     3601 taaaagtcaa aggcttgaaa gaaaaaaata tttcgatagc atcgtttacc cgtgtttatt
     3661 tttctgacaa ataggcatga gaccttaaaa gatagagaat actctagttt tctggccggt
     3721 tgtctgatac aataaagttg attatgataa agaaaagaag gagcaaaaat aatgatctca
     3781 gcaagtgatt taagagccgg tatgactttc gtacaagacg gcaaactgat taaagttctg
     3841 gatgcaagcc accataaacc tggtaaagga aatacagtta tgcgtatgaa actgaaagat
     3901 gtccgttcag gtgcaacata cgacacaacg ttcagaccag aagaaaaatt tgaaaaagca
     3961 catatcgaca caaaacctgt acaatatttg tacacgatgg atgatacagc tttcttcatg
     4021 gatttggaaa catatgagca atatgaaatt cctgtagaaa cagtagaaga agaaatgaaa
     4081 tatatcctag aaaacatgga agtaaaaatc caatttttcg gtagcgaagt catcggcgtt
     4141 caattaccaa caactgtcgt attacgtgtt gtggaaacac aaccttccat caaaggtgca
     4201 acagtcactg gttcaggtaa gccagcgaca atggaaactg gcttagtagt caatgttcct
     4261 gacttcatcg aaacagatga attgttggaa atcaatacag cagaaggttc ttatgtaaaa
     4321 cgtgcgtcaa aataattcgc taacggattg ataagaattt gaaaacagag cttgacctct
     4381 tgaaaggcag atcatcggat ctgcctttca agaggttttt tgtggaatca gcaaattgaa
     4441 aaataaaaaa gtatttgatt ttatttatat tttgaaagat gaatggcaaa agctagagaa
     4501 agaaaaaact gcacttattt atgaagaaat tgaaatgatt cataaaattg aaaatatccg
     4561 aactattccg aactatattg aagcgaaaat attttttcat cactaaatag tgttgaattt
     4621 atgtttttat gtataaatag cttctaatag ttcgggtatt ttgtttttat aaccattttt
     4681 attcttattt ttaaaaaaat atttcgaaat atactagaca attcagaaaa ttttgtttaa
     4741 tattattcca ataaaattca aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa
     4801 gtgaaaagag gaatgaaaaa atgacagaat tgataattgg cggagtcaca gcaagcacat
     4861 tggcagaaaa gtataagaca cctctgtatg tgtatgatga agaaaagatg agacaaacga
     4921 tgctgtcatt caagcgaggc tttgtatcga aacgttttga tacaaaagtg ctttacgcct
     4981 ctaaagcatt ccaaacgatt gagatgctgc atttagttcg tgaatatgga ttcgggctag
     5041 atgttgtcag tggtggggaa atctataccg ctttgcaagc tgattttcct agagatacta
     5101 tctacttcca tggtaacaac aagacgccag aggaactgat ctatgcactt gaaaatgatt
     5161 tgttgcatat tgttgccgat aatttgatgg aagttgaatt attagcagaa ttaagccaac
     5221 gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa gcacatactc
     5281 atgaatatat cgtcactgcg catattgatt caaaattcgg catgagctat gaaagtaaag
     5341 agtgccaaca agcccttcag ttgataaaag aaagcagcta tctagagtta gaaggatttc
     5401 atgctcatat cgggtcacaa atttttgatg ttactgcttg gttagctgaa atcgacaaat
     5461 tgacgaatta cttaaccgat tttgaccaac ctctttcctt gaatcttggc ggagggtttg
     5521 ggattcgtta tacacaaaat gatcagccga tcccagtcga agaggcacta aaaaaactga
     5581 ttgaatatac ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg
     5641 aaccgggtag aagtattgtt ggagaagctg gtacaacact atacacgatt ggtttcatca
     5701 aaaagacacc acataaaaaa tattactttg tagatggcgg gatgacagat aatatccgtc
     5761 cagctctgta tcaggcagaa tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa
     5821 cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag
     5881 cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt ttattccacg ggagcttatg
     5941 gatactcgat gagcagcaac tacaatcgag cgacacgacc agcagttgtt tttgtcaata
     6001 atggccaatc aagactgatt gttcgcagac aagcattttc tgatctacta aggggggaag
     6061 tttcctatga tccttcataa atataatggc tgcggaaatg attttatctt attagattac
     6121 gcagaagaca cagattatag tcaattagcc gcttctttat gcacaaaaga aaaatatgat
     6181 atagacggat tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta caacaaagat
     6241 ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt ttgctcgata cgtaaatgat
     6301 cagaacatga tgaatgaaca ggaattcaac gtagagacat tagcaggcac aagaaaaatt
     6361 acggtgttgg atccctcacc tttttattgc agcgtagata tgggagtgcc agatgattct
     6421 ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa accaggaaat agagatcaat
     6481 ggtgaaaaag taacgatcac tagtttattt ttaggaacga ttcataccgt tgtttttgtt
     6541 gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca tcatcctttg
     6601 ttcaaagaaa aaaccaatgt caactttgta gaagtagtca atgaacaaga attgatcgtc
     6661 cgtacctatg aaagaggagt aggttggaca cttgcttgcg gtacaggttg ctgtgcctct
     6721 tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt tcacttggaa
     6781 caaggagact tgatgatctc tggtgaggat gcgatacaaa tggctggccc agcagttcat
     6841 gaatggacaa gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca
     6901 cgccgatgaa tgaagaggga tcagtagatt atgctggttt ggaaaaattg atccaattcc
     6961 atttagatga acaaacagat ggattattag ttttaggtac aacaggagaa tcttctacgc
     7021 tcacacaatc tgaggaggag cagatccttc aactgacagt aaagaaagtg gctggccgtg
     7081 tgccggtgat tgcaggagct ggaacgaata atacgaaaga aacgattgaa aaagcgaagc
     7141 attttgcttc tttgggggca gacgcacttt tggtcatcac cccttactac aacaaaacaa
     7201 gtgatgcagg actagctgct cattttacag caattgcaga agcctcaccg attccgctta
     7261 ttttgtataa tgttccttct agaacaggca tgtcgattcc aatccatgta ttagtaaatt
     7321 tagcagaaca tcctaatatc attggtttga aagaagcctc aggagatatg gcttatgtga
     7381 tggatgccgc tcggttgatt ggagaagaat tcttccttta cagtggaaat gatgatttga
     7441 tcctaccagt aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg aatatccaac
     7501 ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg aagatggcag caagcaaaag
     7561 aaaagcaatt aaatgcactt gaactgatcc atgccttgtt cagtgagacc aatcctattc
     7621 ctgtcaaagc agccatgtca ttattagact taccttccgg acctctacgc ctgcctttag
     7681 tttctctttc aaaagagaaa aagaaacaat tagctcagct tttgttaaaa gaaaggacgg
     7741 ttgaaaaatg aaaatcggac tgattggcag tggacagatg ggaaatagag tcgaagaagt
     7801 tgcacaagaa agaggagata cggtccttct tttacggacg gcacctaaag aagcaactac
     7861 gcttgctttt acggaactgc cagaactgct tattgatttt tctcatccag ataatttaga
     7921 gaaaatcctt tcgtatagct tatcatatca acttccgtta gtcattggca cgacaggtta
     7981 tacacaatca cagttccaag aaataaaaga acatgccaaa catgtacctg tgatgtatag
     8041 tgctaatttt tcgttcggca tcatggtgat gaatcaacta atcaagcaag cagctgcaat
     8101 gttgcaaggc tggcagatcg aactgattga aaaacatcat agtcgtaaaa aagatgcacc
     8161 atctggtact gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt
     8221 atacaactgg caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc
     8281 aggttcttta cctggtgaac atgatgtatt gtttgctgct actgatgaaa tatttacgat
     8341 caaacatgaa tctttttcta atcgaatctt tgctgcaggc gcagtagaag cagcggactg
     8401 gttgaaagat cagtcaccag gttattacaa attagaagat ttattaatgg acaaaggggt
     8461 gtaaaaaacg tggaagctca agaaatcatc cgctacattc aaacggcatc taaaaagacg
     8521 cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt
     8581 ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga cgattgctcc ggttttagaa
     8641 gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt
     8701 cctttgctag atattaaaaa aattaatgct cgtatcgaac caggtgctat tatcagagat
     8761 caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag cgattatcaa tattggtgca
     8821 gtaatcggtg aaaacacgat gattgatatg ggtgctgtct taggtggacg agcgacagtg
     8881 ggcaaaaact gccatattgg tgcaggagct gtcttagcag gtgtgatcga accagcatcg
     8941 gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt gattgttgaa
     9001 ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg
     9061 gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat taaaaataag tgatgatcag
     9121 acaaaagaaa atacagcatt gattgctgcg ttacgcgaat tatagaggag atggagaaaa
     9181 atgcgcgaaa tagatacctg tattgtgggg gcaaccggat tagtcggcca aacgatgctc
     9241 aaagtgttag aagaatatga ttttccagta ggacgattga aattattagc ttcaaaacgt
     9301 tcagccggta aagtttgtgt attcaagaaa aagaaacaca ctgtacagga attgacagaa
     9361 acttcatttg aaggatatga tttagcgcta ttttctgcag gtgcttctgt atccaaagag
     9421 tttgcgccaa tcgcacgaag tcagggacta tttgtcatag ataattcctc agcatggcgg
     9481 gaagattcgg atattccatt aatcgttcca gaggtcaatc tggaagacta tacattgaat
     9541 cggctgattg caaatccaaa ttgttcaacc attcaagcgg tacttccgct taaagcttta
     9601 caagaagcct ttggtatcca gcgagtcaat tattcgacct atcaagcagt ttctggttcc
     9661 gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc agttttgtat
     9721 ccttataata ttagccaaac ggtgattccg gaaatcgatc agcctttgga aaatgggtat
     9781 acaaaagaag aacagaaaat gataaatgag aaccgtaaga ttctgcatca gcctgctttg
     9841 ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg gacatggagt ctctgtggca
     9901 gttcaactgc aaaaagagtt tacgacggaa gaaattagag agtgtctcag ccagtttcca
     9961 gggattcaat tagaagatga tctagcagca cataaatatc cgacgagcat tcttgctaga
    10021 ggaacagata tggtttatgt tgggaggatc agaaaagaca cttctttaga gaacggattg
    10081 ttgttctata cgactgccga taacatacgc aaaggagccg ctgcgaatgc cgtacagatt
    10141 gctgcagctt taatgcagaa aatagcggag gtggcaagat gagtaatacg ctgaaagtag
    10201 cgaaattcgg cggaacatcg atgtctcatg aagagcaatt caaaaaagtc cgtacaattg
    10261 ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag
    10321 atgatgacaa agtaacagac ttactttatt tgatttatgc ttaccttcag catcatgttg
    10381 attggacacc cctttggaaa aggatttgtg atcgtttcat tcaagtaaga gatgttcttg
    10441 ccctgcagtt acctattgaa gatgaactag cacgcataca gaaacaactg gaagacgaaa
    10501 agataactga agattattta gtaagtagag gtgaatatct gactgctcaa ctgatggctg
    10561 agtatcttgg ttaccagttt attgacgcaa aagacctgat tttctttgac tatgatggga
    10621 aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa
    10681 tggttgttcc agggttttac ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg
    10741 ggggaagcga cattaccggc gcgatactgg cgaagatgtt gcaagcggaa agttatgaga
    10801 attggacgga tgtttcagga atcatgatgg ctgatccgcg aatcatcgat catcctaaaa
    10861 tcatcgagga aatctctttt agagaactga gagagatggc ttatatgggg gctaacgttc
    10921 ttcatgaagc agctgttttc ccagttcaag aggctgatat ccccatccaa ataaaaaata
    10981 ccaatgatcc tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg
    11041 gactgacagg aatagctgga cgcaaggatt ttctttccat cactttattc aaacgtcata
    11101 tgtccgatga aatcgggttt atctggaaag caatgagcat ttttgctaaa catgagatat
    11161 caatcgaaca tattccttca gggatcgaca atatcggagt gatcgtctca gcagaagcga
    11221 ttgccgataa gttatttctc atcacgaaag aattaaaaga aacacttggc gtggaagaaa
    11281 tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg gggtccgcat aaagaattga
    11341 ttggattatc tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc acatcgatct
    11401 tatcacaagg agcacaggag ttgaatctga tcatcggtgt accgaataat caatatgaaa
    11461 cagtagtgaa gggtatctat gaagggatgg tgaatacgga tgctgaaaga atatcgaatg
    11521 gcactccatc aaattcctga aatcgggttt catgaattca agacaaaaaa atatttatat
    11581 gaacaagtaa aaaacagcga ggccgttatc catgaaatcg gcgaaacagg actactcctt
    11641 tattttgata atcagcaaga agaaacgatt gcttttcgaa cagatatcga tgcactgcca
    11701 gtcaaagaag aaacgaatct ctcttttgct tctaagcatg aaggttatat gcatgcatgt
    11761 ggtcatgacg gacatatggc gatgctttta gggttgactg actttataac taaacacatt
    11821 gaggatctta tttataatgt agtactgatc tatcagccat cagaagaaat ttccggtggc
    11881 gcacaaagcg ttattcagtc aggattgttg gataaatatg aagtacaagc aatatttggc
    11941 tttcatctat ggcctggact agctaaggga gaagttttct ctcgaccagg acctttgatg
    12001 gcacaaagca gtgaaacaga cattatcatt aaaggccagt ctgcacatat tgcttctagt
    12061 gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt tgaagaatgt ttatgatttt
    12121 gaccggcaat taccggcaga gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga
    12181 acgatccgaa atgttttagc tggagaagtt attgtatccg gaagtatccg ttcttacagt
    12241 tcagatatgc aaaattattt gaagagggga ttagaccatt tagcagaaca gtttcactct
    12301 acagctacta tttcttttcg ctataatgac ggctatccag ctgtaatcaa ccatagaggc
    12361 ttatatgcag cactgttaga ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa
    12421 gccgaagatt ttggcatata tacgcaacaa tatccatgtg tatttttctt tttaggtgtc
    12481 ggtgataccc ctgctttaca tgatccgacg ttcgattttg atatggatgt tttagaaaat
    12541 ggtgtagaat ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa aatcgatagc
    12601 agttcaaaag aggagcgagt aaataatgat gaatttatcc aatcgattta gtcctaggct
    12661 ttctagaata gaagtatcaa aaatacgaca attcgatcag caaatatcgt cgataccaga
    12721 tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact cctgaacatg tcaagcaagc
    12781 aggaatagca gcgattgaag aagacttttc tcactatacc ggaatgagag gattagagga
    12841 actgcgggaa gcggcttgta tatttcagca acagcgttat ggattaactt atgatccgca
    12901 aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt
    12961 tttggaagaa ggagacaagg ttcttattcc agcgcctgct tattcaggat atcagccatt
    13021 agtggaacta gctggtgctg aattgatccc gatagatact tctgacacag ggtttgtctg
    13081 tcagccggaa caatttgaac gagcatttga acaatatgga tcagctgtca aagcagtcat
    13141 tttaaactat cctaacaatc ctactgggac gacattgtct gcaaaacaat tgaaagcaat
    13201 cgctgaggtt ttaaaaaaat atccggtatt tgttataagt gatgaagtat atgctgaatt
    13261 gacttatagt ggaacgcata tgtccatagc tacttatctt ccagaacaaa caatcgtgat
    13321 aagcgggtta tccaagtcac atgcgatgac tggttggcga gtgggcttta tttttgctca
    13381 aaaagccctt attgacgaac taattaaagt ccatcaatat ctggtgactg ccgctacaac
    13441 gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg gtgaaagata gcgagaatat
    13501 gaaagagcaa tatcaaacac gaagagacta tctggtccag caattggcgc ctttaggatt
    13561 tgaagtgacc cagccaaacg gtgcatttta cttgttttgc aaactcccag ctgccattca
    13621 aactgactct tggcagtttt gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc
    13681 aggaattgca tttggcccag aaggagaagg ttatatccgg atttcttatg ctagcagcat
    13741 ggaaaaatta catgaggcct gcaaacggat caaagacttt ttaacagaac agtgattcat
    13801 agattttatc aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga gacgattgtt
    13861 ttttaaattt agttcaaatg aacgcgtttc ttttgattca taactaatgt aatgttaagt
    13921 ttaaattgtt tagaatgatt ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat
    13981 caaagttgtc attgtaggtg catctcatgg agggcatcaa tcgatcttgg aactattgac
    14041 acgatacggt gaaaatgtag atatcacgct atttgaagca ggagactata tttccttcat
    14101 gtcatgtggt atggagctgt atttagaaga caaggtaaca gatgtaaatg atgtacgtaa
    14161 tttcagacca gaaaactttc cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa
    14221 aacgatcgat gccgataaaa aaacagtaac ggttactagc gcatcagata acgaaacaaa
    14281 agaatatcct tatgacaaat tgattttgag ttctggtgtc aaaccaaaca atctgccagt
    14341 accaggagca gatctagaaa atgtctactt gatgcgtgga tacaattggg caaccaaaat
    14401 caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg cgggttacat
    14461 tgggatcgaa gcagcagaag caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt
    14521 gatcgataga cctttaggca cttatttaga tgctgagatg acagatattt tagaacaaca
    14581 cctaaatgaa aacggtattg aagttttgac aaatgcaaaa atcgaagctt ttactggtaa
    14641 cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc ccagcagatc tagtcattca
    14701 agctgccggt gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact tagatgaacg
    14761 tggctggatc caaacaaatg aatacttgca aacgaatctt ccagatgtgt atgcagtagg
    14821 agatgctaca cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac
    14881 tgttgcacgt agagaagcac gttatgtagt aaaacatctt tttgaagaag taccaaacaa
    14941 accgttcggc ggagtcgtag gttcctctgc attaagtgtc tttgactatc attttgcagc
    15001 aagcggattg aatagcttta cagcagcaaa agcaggagta gatgtacgag tgtcttatta
    15061 tgaagatacg ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa aagtgtccat
    15121 ccaattattc tcggatccaa tgacccatca attgctagga ggagcagtat tatctaccta
    15181 tgatgttaca ggtcaaggaa atgtattggc actagcgatc caaaacaaac tgactttaga
    15241 agaactagca gaagctgact tcttcttcca gccaggcttt gaccgacaat ggagcatgtt
    15301 gaatctagcc gctcaacaag cgttaggaga agaaccattt gtagaataag aaaacgaaat
    15361 atttgaaaa
//
LOCUS       DACEMO010000054        15003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEMO010000054 DACEMO010000000
VERSION     DACEMO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.055"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(100..414)
                     /locus_tag="IW728_001268"
     CDS             complement(100..414)
                     /locus_tag="IW728_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1024638.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(428..688)
                     /locus_tag="IW728_001269"
     CDS             complement(428..688)
                     /locus_tag="IW728_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1024639.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            858..1172
                     /locus_tag="IW728_001270"
     CDS             858..1172
                     /locus_tag="IW728_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1024640.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1174..2829
                     /locus_tag="IW728_001271"
     CDS             1174..2829
                     /locus_tag="IW728_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1024641.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2912..5047
                     /locus_tag="IW728_001272"
     CDS             2912..5047
                     /locus_tag="IW728_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1024642.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5222..5731
                     /locus_tag="IW728_001273"
     CDS             5222..5731
                     /locus_tag="IW728_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024643.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            5728..7032
                     /locus_tag="IW728_001274"
     CDS             5728..7032
                     /locus_tag="IW728_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1024644.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            7128..7997
                     /locus_tag="IW728_001275"
     CDS             7128..7997
                     /locus_tag="IW728_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1024645.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8109..8429)
                     /locus_tag="IW728_001276"
     CDS             complement(8109..8429)
                     /locus_tag="IW728_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1024646.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            8583..8978
                     /gene="dhaM"
                     /locus_tag="IW728_001277"
     CDS             8583..8978
                     /gene="dhaM"
                     /locus_tag="IW728_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1024647.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            8980..9963
                     /gene="dhaK"
                     /locus_tag="IW728_001278"
     CDS             8980..9963
                     /gene="dhaK"
                     /locus_tag="IW728_001278"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1024648.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            9991..10596
                     /gene="dhaL"
                     /locus_tag="IW728_001279"
     CDS             9991..10596
                     /gene="dhaL"
                     /locus_tag="IW728_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1024649.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            10743..11408
                     /locus_tag="IW728_001280"
     CDS             10743..11408
                     /locus_tag="IW728_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024650.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            11691..12350
                     /locus_tag="IW728_001281"
     CDS             11691..12350
                     /locus_tag="IW728_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1024651.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12427..14037)
                     /locus_tag="IW728_001282"
     CDS             complement(12427..14037)
                     /locus_tag="IW728_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ1024652.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(14050..14931)
                     /locus_tag="IW728_001283"
     CDS             complement(14050..14931)
                     /locus_tag="IW728_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024653.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
BASE COUNT         5051 a         2740 c         3109 g         4103 t
ORIGIN      
        1 ttttcacaac ctttgattca cggttttcca gaagtagctt cttcgaaaaa taagccgaaa
       61 tttcttgaag ctgatcactt ctgtcgcaac ctctactgac taaggcttga tataagcata
      121 accttgggat tgcagttcaa ctaaatcaag gacacctgct ggtacaacga ttacttgttc
      181 aggtaaagaa ctttctgaaa tatgattagc acgcatagca ttggcgcaag cgtgaaaaac
      241 gactccttgc aaatctaaga attctgcatt ggcagagtct agataaccgg taatcgcgat
      301 gccatttaca gatacgacaa tcgtcgtttc tggagaagct tttatcaggt tttttacgtt
      361 tttccctgtt tctgaccatt tttctaattc atcaatatgg aaaacgactt tcattgaaat
      421 cattccttta ctttttaata atcatcttga tcgcttgttc tagtttagcc gcttgttctt
      481 ctggattact atcaggtttc tcaaagcaat ttttcagatt atctgcaacg atcatgccca
      541 ttacgcgatc aatactagaa cgaacagcgc ttaattgagt gatgacatcc atacattctt
      601 tttcgtcttc gatcattttt tggataccac gaatttgtcc ctctgtacgt ttcaaacggt
      661 tcaggatatt tttattatca atctccataa cacattctcc tctattttta taattagata
      721 atacccctta tcggtatttt atgcaaatat aaaaagtagt gaaaaaaatg aagagtcatt
      781 ctggttgaca tattaataaa gctcctgttt aatatacccc atgaggtatt tataaaaatg
      841 taaatgaggt gcagaaaatg aatttgctat ttcaatcaat aaaatcgata acagtcccag
      901 aattaaagga aaagcttttg gaaaatccag cgttacttga tgttagaaca cctgcagaat
      961 accggggcgg gcatataaaa ggagctaaaa atgttccatt acaaagtata aacagatatg
     1021 atggagacaa agaaaagacc gtctatgtga tttgtcaatc agggatgagg agtaaacaag
     1081 cagctaaaga attaaagaaa tctggttatg atgtagtgaa tgtacgtggt ggcatgaatc
     1141 aatggtttga tcgaacagta ggagggaaat aagatgaaaa tcgttattgt cggaggtgta
     1201 gcaggtggaa tgtctgctgc tacacggctt cgccgattaa tggaagatgc agaaatcgtt
     1261 gtttttgaaa aaggaccgta tgtttctttt gcaaattgcg gcttgcctta ttatctttcg
     1321 ggagaaatca gtgaacggga aaatcttctt gtccaaacgc cagaatcatt atccgctcgt
     1381 ttttgtttag atgtgcgtcc aaatcatgaa gtgacagcca tctttcccga aaacaaaacg
     1441 gtagaagtcg tacatgaggg tcaaaaacac attgaacagt acgatgcatt ggttttatct
     1501 cctggtgcaa aaccagttgt tccatcgatt ccagggataa cagaagccga caatgttttt
     1561 tctattagaa atgtaccaga tatcgataaa gtgatgcatg cattagaaaa acagccaaag
     1621 cgtgccgtga tcgttggtgc aggattcatc ggattggaaa tggcagaaaa cctaaaaaga
     1681 agaggtttag aagtcatggt gatcgaacaa gcaccacata ttcttccaac actggatgaa
     1741 gaaatggcag cttttataga aaaagaattg tctcatcaag gagtagaagt gattacttct
     1801 catgctgtcg ctggatttga agaccacggg aaacgattgc gactggatga tgggcgtacc
     1861 atccctgctg atttagttat tttatccatt ggtgttcgtc ctgataacca gctagcagtg
     1921 accgctggaa tcgaattagg catacgcggg ggtatcctag tagacgaacg ttatcaaacg
     1981 aatattcctg atatttatgc ggtgggggat gctatcgttg taaaacagca aatcactgga
     2041 aaagatgcac ttatttctct tgcttcacca gccaatcgtc aaggtagaca agttgcggac
     2101 acgatttccg gaatttctcg aagaaatcaa ggcggtattg gaacagcaat tatacgaacg
     2161 tttggaatga ctgccgcatc caccggttta agtgaaagaa cagccaaaga aaacgaactg
     2221 tcttttgaag tcattcatgt atcaggaaaa gatcatgcaa gctattatcc agaagcaaca
     2281 gatattttac tgaagttgat cttccatcca gagactggcg agatttatgg tgcacaaggt
     2341 gttggggcaa aaggtgtgga taaacggatc gatattttag caacagcaat caaagggcat
     2401 ttgacgatct tcgatttacc ggaattagaa ttgacgtatg caccgccatt tggctcagcc
     2461 aaagatccag taaacatgct aggatatgca gcaatgaaca ttgcagaagg gcttagtgaa
     2521 accgtacaat ggcatgaatt gccgacagaa ctagcaaaag gaaaaatttt attagatgtg
     2581 cgaacagcag aagaattgga aaagggcaaa ttcaaggaag ccaaacatat ccctttgaat
     2641 gaacttcggg accgattaaa tgaattagac agccaacaag aatatatcgt cagctgtcat
     2701 agcggattac gtagctatct agcggaaaga atcttgaagc agtctggcta ccacgtaaaa
     2761 aaccttgatg gtgcattttc tttatatcaa actgtccgac ctgaagaact gatatatcct
     2821 aacaaatgat actcgttaaa aagcttgcat tcgactctta aaagaggtaa actaatcggt
     2881 tccaccactt taaaaaaaga ggagaatgtg tatggtcaac acacaaaaac agttagaaga
     2941 agcatatctg aagaacgtgt atcaaaagct aattgataaa aaaaacgaac tgcgggaatc
     3001 gatggatacg gcaaaaatag aaggaaacca gtcgttgaaa gaaatggctg gcgagcttcg
     3061 attgaatttt gacagttact tagataacct ggatactttt tcgatgattg aaatgaaaaa
     3121 tagagaaatc gatcagatga atatcaaaca gaaaaacgca gaaaatcagc tagcaaagat
     3181 cgaacgttta ttgaaaagtc cctactttgg aaaagtcgtt gtagactttc tggataatga
     3241 gccaaaagaa tccttttata tcggtacagc aaatttcaca gatgatgcag atgaaaatct
     3301 tgtttatgat tggcgttcac caattgccga acttttttac aataacatga ttggtccatc
     3361 ttcttatacg gttcgtcaaa ataaaatcga tacggttatt aaagaaagac gacaatttct
     3421 tttggaaaga gatcgtttgg tcaagttttt tgatacgtct gtagctattc aagatgacgt
     3481 attattagaa gtcttaggcc aagatgagac caatgaaatg aaggctatca ctgctacgat
     3541 ccaatcggaa caaaacacaa tcattcggga tgtgaaaagt caaaatatct tggtaaatgg
     3601 cgtagcagga agtggtaaaa cctctgctat catgcagcgt attgcgtatc tgctctattt
     3661 atatcgagat acgatgacat ccgatgactt gctgattcta tcacctaatc atcggtttat
     3721 cgactatatt tcgaatgtcc taccatcatt aggtgaaaga aatccactga atctaaccat
     3781 catacagctg gtttcacagc tttcagcaga ggaaatagaa ggtgaagagg cttatttcaa
     3841 acgtatcacg ggagaaaatg tatcggagca gacagaacgt ttgcgtagca aaaagtttat
     3901 cgataatcta aaacaatcag atcctttgtt tttagatcat ccgaatttta tccgtggact
     3961 tactaaaaat ggaaaaacag tcctttctaa aaaaacgatc gaaaaaatat atgagaaagt
     4021 tcctgctcat cctaaattga tcgataggct tcaagcaacg aaaaaggcac tgatgagcga
     4081 atggaagaat catcttttga aacaagcaaa aagtcctgct gtgcagaatc aggtactctc
     4141 gttgacagaa gataggcagt tggaattatt cggtaaattg atttctgatg attcggaaca
     4201 aagtattgta gcttatgcaa ggaaactttt acaaaaaaaa tatcgaaaaa taaccagaca
     4261 aatcgaagaa atggcatggg tggatgagca tcagttgttt gaaagaattt atgaaaaacg
     4321 atatggctca gcttatgctt ggcaacccac acgtactgtt gatgaagcag tcattatatt
     4381 agctattcgt catttacttg ttgaaaaggt aaatgtacca gcatttcgct atctattgat
     4441 tgatgaagta caagattata ccctggcaca attaggtcta ttgatcgaat tatttccaaa
     4501 aactcacttt actttagtag gtgacgagaa tcaggcaatc tttaattctt ccactacttt
     4561 tgctgatatt atgcgttgtt tcgacgacta ccatctgccg atccatcggt atgatcttag
     4621 aaatagttac cgttctagcg gagcaatcac agagcttttc aaaacctatg ctgtggatca
     4681 agagaagatc gacatcgtat cgatccgacc gcaaggaaaa gaacccgaat accgctcttt
     4741 tcgatcctct gaagaattgt tgaaaatact agctgagatt ttggtttctc taaaaggaga
     4801 aaaagcggca gttatcaccc aaacagaaga ggaaacccaa acgctacaag aatcgattaa
     4861 aaaaaattca tatggattag aaaactgcca aatcattcct ttgagtctag caaaaggact
     4921 ggaatttgat catgtaatct tatatccatt tgaaaacgat ggagatgagc aaagaagacg
     4981 cagacaaatg tacacggcga tatctagagg aatgaaaagc attgtcgtat tggaaagagc
     5041 aacataatta agagataaaa aataaaaaga acaactgata aaaagaacaa aatatttttc
     5101 atttgctgat gagaaatgtt ttgttctttt tcttaggaaa aatttaggaa cactttatgt
     5161 gaaaagaaat gaaatgtatg gtaaactcct acgtcgtaac aaaaaaacgt gaggtgagga
     5221 tatgaaaaaa gtaacaagag aagaagttgc atcaattttg gtgaaagata agtatttttc
     5281 aaaaggtaat tactggttaa agatttggca aacgttagtg gctcttttag gatggatgat
     5341 cgttgttctt ccttttatat gggtcttttt cccattaacg agaccagaaa gagccaagga
     5401 tttttccttg tacgcttttt tatcagaaaa gaaaatgctg tatttcttaa tcggcttttt
     5461 tgtattgatt tttttcagtt tcctgattac ctcatttgta ttgactaggt ggaacaatcg
     5521 aaatctatac aaaaaagtaa ataaatcttt tgaatatgaa gatgaaaaat tgaaaaaaag
     5581 acaagaatta cttgaagaag tgtatacgga acgttttgga acaaaagaag agagagaaac
     5641 ggtccgtttt tattctgtct ccgaagagaa aaatctagat acgacattca ttgcagattt
     5701 gtataaagaa aatggggtgg aactgaaatg atctcaatca cccatacagg taatttattc
     5761 ttagatactt gtttgtctat tatgtatttt tttctcgttt cttatccgat tttaggcgga
     5821 tttgtatggt ttattggtgt ctggtgttat gtgtttttat acaaacataa gcaaaaagaa
     5881 tgggtagatg ttcccttgag tgtcgaaccg ttcatcacga tcatggtgcc agcccataat
     5941 gaagaaattg tgatcgaata tacaatcgag tatttgatga cgaaattgaa ttatcacaac
     6001 tatgaggtat tagtgacaga cgatggttca acggaccaaa caccagaaat attagctaga
     6061 ctgatgaaaa aatacgctaa tcttcgtgtg gttcggatcg aaaaaaataa aggaaaagcc
     6121 cacgcgttta atatcggact tgcctttgca aaagggaaat tgattttaag caatgatgca
     6181 gacacggtac cggagccaga tgcactgatt aggtatgtga attatttcat tcgccctggt
     6241 gcaagacata ttgctgcggt cactgcaaat atggatgtcc aaaatcgtac caaattgatt
     6301 gcaaaatctc aaacggttga attctcaagt atcgtgggca tcattaaacg aacacagtca
     6361 gctgttttcg gtggtcttta tgcgtacagt ggagcaaata cgatgtatcg aaaagaagcc
     6421 ttgatcgatg tgggcggatt cagacaagat cgagcgacag aagacatcag tattgcttgg
     6481 gatcatcagt taaatgactg gttatctgtt tttgctcctg agattatctt tttcatggaa
     6541 gtacctgtga cgttgaagat gctatatcga cacagaaaac gttgggcaaa aggtggaaca
     6601 gaagtctggc tgacgaattt caaaaaagtg atgctccatc cttttaaaca tattggaaga
     6661 acgatcatct tcattgatca aacattgagt attgtctggt caatattctt ttgtatttcc
     6721 gtagttcttt ttgctggatt aatcgggcat tatgtatatc aaggaaacta cgaacagatt
     6781 tatattactt ttacattcag ttttgtgttc atctgctttg aaatggtcgc aggatttttc
     6841 cagttgctag cttcgctgat tgtagatgat cgacgaagaa aattgaaata ccttcttttc
     6901 gcaccactat atatgctttt gttctggata gtgaacgcga taacgatcgt gacgactttc
     6961 attccagcag tcaaaacgat tttaggttat ggaagcggga catggaaaag cccggaacgt
     7021 acaaaaaaat aaaagatgca ccgtttttta gacaaacagc ctgttgtgtt tatggttctt
     7081 ccaaacattt ttgcttataa taagaaatta gttaaaaagt agtaaggatg ggaaaagtga
     7141 tgaaaaaaag aatgaagccg ttaatctggc caattgctgt ttccagtatt ttcttattgg
     7201 gacagacagc ttttgcggaa acttctgaac ctgctcaaac agaaccttca acaaccataa
     7261 gctcttctgg tgctgaaaac ggtgcctcgg aaacagaaat gacttccaat gtaccgacag
     7321 ataattcagg agaaacgatt tcatcttcag aagatacatc aataaatagt tctacctctt
     7381 ctgaccaaat cgatcatttg caagacttgg aaaataaaag aaaagaactc gaagaagcat
     7441 tagaagctgc aaaaaacaaa ttgaaagaag accaagcagc aatcgatcaa gatttagctg
     7501 atcttaaaaa taaagtggga aatgaccgtg cacaacttga gaaaaagctt gccgaagcga
     7561 aagcaaaagc ggaagctgtc caaacagatc ttgacgggaa gatccaacaa gcaaaagaat
     7621 tcaatcagca attaaatcag gttgctcaaa cagcttctgg aaagctaagt caaactcaag
     7681 cggatatcac agcggccagt caagcaatcc acggaaagat cagcgaagca caaacagttg
     7741 ctaatgattt tgaagcaaaa aaagctgact tagcagctag tttccaacga ataaaagata
     7801 ttttgcattc ggcaggagaa aaatcagtga cctctggaag cacgacagaa acagttcagc
     7861 aaacgacaga gccatccaaa tcaacaagtc agactttgcc aaaaacaaat gaaaaaacag
     7921 cttctgtcag ttattcctta gcaggggcgg gtgtgttagt cgcagcagct tacggaatca
     7981 tccgcaaaaa gaaataaaaa agagaggatg ggaaggtaga gtacctcgaa gttagactga
     8041 aaaatctaat tcttgagtgc cactacatgt cccatctctc tttagttatt aaaacagatg
     8101 tcccctactt atctcgtgga gacagctgtt ccactacatg tgaccatcag catgccattg
     8161 tcgctgccta atacttcata gtccattttt acgccaatga tcgcatcagc tcctaatgct
     8221 tttgcacgtt cggccatttc ggcaagtgct tcttctcttg cacccattag ttcgttttca
     8281 tagctttttg agcggccgcc aaagatattt cgcaaccccg cctccaagtc tttaaatgca
     8341 tggatcccag taatgacctc accaaaaacg atccctttat aagctactac ggcttttccc
     8401 tctacactgt tcgttgttgt tgtaatcatt cgatcactcc tctcttttaa ttaaagtata
     8461 acaaacttca agaagcacta cacatatttt cccaaaatta gaaatttatt atatacttga
     8521 attgttcatt aatgaaagcg aaaccaatcg ttagcaacta tgtaaaagaa aggaagattc
     8581 ttgtgaaaaa aagtattttg cttatttctc atagtcaaaa agtaacagat ggtattaaag
     8641 aaatgattga gcagatgcaa aaatccgaag aggtgcaaat tttttctctc ggcggaataa
     8701 atggcgagat cggatcagac ccgatgaaaa tcgtagaagc agtcaatgaa tcaagcgatg
     8761 cagctagtta ttttgttttt gcggatatcg gtagcgcggt attgaactcg gaactggcaa
     8821 aagacatgtt ggaagaagag cagcagcaac gttatcatct ggtggatgcg cctttagtgg
     8881 aaggagcttt tgcagcagcc atcactgccg gtataacaga tgatactcag caaatcttaa
     8941 cagaagcaca aaatgccgga aagaaaggat gggaataaat tgaaaaaaat catgaatgat
     9001 ccaagcaata ttgtcgaaga aatgctggaa ggattagtaa aaagctatcc agaacttgtt
     9061 catcgtgtag aatcttcccg tgttgtagcc aagaatcaga aagcggaaca agttggtttg
     9121 gtttctggtg gaggaagcgg acatgaacca tcccatgccg ggtttgtagg ggaagggatg
     9181 ttaagtgcag cggtgcttgg tgatgtgttt acttctccaa ctccagatca gattcagacg
     9241 gctattaagg aagccgatag tggaaaagga gttttgctga tcgttaagaa ttatacaggg
     9301 gatgcattga acttcgatat ggcaaaggaa ttagctgcta tggacgatat tgaagtagaa
     9361 agtgtgatcg tagatgatga tatcgccgtt gaaaatagta cgtatactgc tggaaaacgc
     9421 ggagttgcag gtactgtcct tgtacacaaa atcgtgggcg atgcagcaag aaacggtgct
     9481 tccctagctg agttgaaaga actgggagaa aaggtcgtac aggcaactaa gacgatcggt
     9541 ctagcgcttc gagcagcaac tgtaccagaa gtagggaaac caggattcga actaggagaa
     9601 gatgagatcg agtatggtgt tggtatccac ggtgagccag gctatcgtcg agaaaagatg
     9661 cagccttcta aagtactggc aaaggaactt gttactaaga ttttagatga ttactcgaat
     9721 ttaccaaaag aagcaggtgt actagtcaac ggaatgggcg ggacgccttt gatggaacag
     9781 ttcgtgttca tgaatgatgt tttagctctg ttggaagaaa gaggcgtcaa tgtagttttc
     9841 cgaaaagtcg gtaattttat gacctcacta gatatgcaag gtctttctct aacactgatc
     9901 gacttgacgg agacacaatg gaaagattct ttagaaagca atgtacagac gatttcatgg
     9961 taaaaaatct caagataaaa gggtgataat atgaaactaa ccgttcaaga aatccaagaa
    10021 tggctcacgc aatttgcaga ggcaatcaat cagaacaaac aatatctcag tgatctagat
    10081 accccgattg gcgatggaga ccatggaaat aacatgggaa gaggggtttc agcttatgaa
    10141 gaagcttttc aagccgatca tcctgaaaca atcagcgata cattcaaagt attctctatg
    10201 gcgatgatat cgaaagtcgg tggggcttct ggtccgttat acggatctgc attcatgaac
    10261 atgatgaaag cgacaaaaga ggtcgattct attgattctc aagaaaaatt aggcgaagtg
    10321 atcgagcagg gaacagcggg tattcaaagc cgaggaaagg cagaagctga cgataaaacg
    10381 atgttagatg tttggctgcc agtcacagag gcattgaagg cagggaattt aacaaaagaa
    10441 atcattgacc aggcaaaaga acacaccaaa gtcttggtag ctaaaaaagg acgagcttcc
    10501 tatctaggag agcgtgcaat cggacatatc gacccaggag ctgcttctag tgccatttta
    10561 ttttcaacgc ttttagatgt gatcaatcaa ccttaagttc aataaaactg cggtgatgct
    10621 catcgcagtt ttttgtttgg ctgttcttat gacttgatgc agaacagctt tttcacaacc
    10681 tctatttgtc catatcgcaa aattcaggta gaatacaaaa gataacggaa agaggcgaaa
    10741 caatggaact tggaaaatca gattatcagt tgtttgatcg accaatctac gcattcaaac
    10801 agctcaaaga gagccatcca acagataaga ttgaacaaat caagaaagaa tataaagaac
    10861 actggcaaaa atggaaagag attcagctcc agacagctgc acttttgcct gatatgtacg
    10921 gcatgagcaa acctaaaatc gaaagctgga cgaatggctg gaatttacgc agtcattttt
    10981 ggagcgcgta ccgaagcgaa tctcgtcaag acgagaatgc ctgtctagca gttttgttga
    11041 atcaaaaaca ataccaaatc tatttgatgt accaacatta taaaagcgaa gaacgatatg
    11101 gtagcattgc agcgtataac cagttgcttg cgatactgaa agagtggagc aaaacagtcg
    11161 atatcaagga ttattacata tggccgcaac cagaacatga acttgacgac cacttagctt
    11221 tgtctgacta tttgtccgat acaaagaaac aagaagagtt gaaaaaagcg atgagagacc
    11281 gaacctttca aatgggtaaa ctattcttct atccgcaaga aatcgaacac gttgagcaaa
    11341 taaccgctga aaccttacaa gaactagcac cactatatca aaagctaggt gcggaaaaat
    11401 ttcaatagac aacacgaaat aagcaatgcg aaaaacaaac cttactctcg gcttgcgagt
    11461 gaaggtttgt ttttgattat tcaaaaagta aatatttaaa aaattgatag gaatcaatta
    11521 cttaaggtct caaaagaagg aaaataaagc aagggacaaa aagaaatcca ctctatttgc
    11581 tggtatctgg ttcgcttgac gtttatgcta tgataaaaga aaattatcag ttggaataag
    11641 ggaagttatg tttctttatt agaaatagct gtttagaagg aggagagatt atgagtcatg
    11701 atcatttatg tgtttcattt gttcctttgt tcaatcactt agtacagcaa gatcaagaaa
    11761 aagtacacgc acttgtcaaa catcactatg tggaaaaagg agaacagatt cttgcgccga
    11821 atggagaacc tcaattagtg atcgtggcct ccggcagtat gaaagtgtat caattatctg
    11881 ctagcggaaa ggaacagctt ttacgagtaa tagagccggg cggatatgag ggagagagcc
    11941 agttgctagg cgttccaaat gagtctttat ttggcgaagc actagaaaaa acggaaattt
    12001 gcatattgag acaaaaagat ttctcggaac tcttactgca ttatcccgcc cttagtctga
    12061 aacttttagc aatcaacgtt gagaaattaa caaaagtaca gcagcagaca acttttttga
    12121 tgatggaaaa agtagaagaa cgagtcgctg tctatctgct tgacttagct aaagcgacag
    12181 ggaaggatca ctttttcctg ccaatgaaaa tgaaagaact tgctgcattt atcggtacga
    12241 cccccgaaac tttatctaga aaatttaaat tattagagga gaatggttat gtaaaaagaa
    12301 agaatcgaga agtctggatc attgataaag agcagttaga agacttgtaa ataaaaaaat
    12361 gacctccaaa tgttggattt ttgacccaga tttggaggct agcatgaaat tgtacatgct
    12421 tttttattat ccttctagta agtctcttcg cctataacca atagaaccaa ggacgaacag
    12481 tgcaaggctg atacatgata acgtaaggaa cacggaaaga tcaaaagaat cgtttggcat
    12541 ccgtggaacc cagctaggga cagctgtttt ggaaaaccat tctggtaaat ccaaaattcc
    12601 gccaaagtaa ttgagtgcaa aggagtaagc taagtagata taagcaagtt taccgattcg
    12661 aggaagccat ccgaatagaa agccactaat tccgataaac actaaaacgg caggcaggaa
    12721 attgaacccg gctttaagaa acatcaatag atccatgtct ttcgcatcct tcatcgaagc
    12781 caaagcagaa agccctagtg caccagaact aatcaggatt gccaatatag caaatagaat
    12841 agctagtaac atattggtcc aataaagttg gttcctagaa atttttgttg cgaacagctg
    12901 attcatacgt ccgtgggttt cttctgtaaa taaacgattg atgctagcaa ttggtaaaat
    12961 agcagctaga caaatcaaga ctaccatgat cgtgcttgta aagttttttt caattgagac
    13021 gccagattgg ataaacatct gtttcattaa gtcgttactt tctaaaaact gttgcatatt
    13081 cccgtagata gagccgtatg ccgcccccat caaaccaaaa gtgataagcc aactgattgt
    13141 catcccttta tttagataga ataataaacc acgcaaggat aaaagagaac gtttagcata
    13201 cggttttcct tcacgttcgg ggatatagcc ggtgttcaaa tctcgatgtt cctctaaaac
    13261 aaatgaactg attatcaaga ttagactgaa caaaaacaaa taaagcaaag gaatccagtt
    13321 gttagttgta aatggatagg tcaaataaga ccagcctaaa ggattccaca tcgaaaggga
    13381 ggaatcagct acatctgtta ttgctcgtag aaggtaaagt ccacctaaaa acgctaaaga
    13441 cagaccattc gaactggctg aacttggcat cagttgtgca gcaaacagtg caacggcact
    13501 ccccaaaatc cccatactac ctacagatag cccaaaaagc caagcacctt ctgcagtgat
    13561 cgaatccgct tggaaaaaaa tcatcgtaca gctgatcgat attgctaaaa gcaaattgat
    13621 cagaatactt tcagcgatga cagctaaaga gtttgcttga cgtccgactc gaaaggaacg
    13681 aataagttca atcagaccaa gttcttcttt ttttctcgtt tggctcacca cgtgtaaaac
    13741 agagatcaca gcagcaaaca aactgcaaaa aagcaacatt tcatttgcat acatcgctcc
    13801 taaagtatac tcagcagcat tcgaagctgg agttggtccg accattgcaa tcattgccgg
    13861 gttttgcaag gtttcgtata atccagctaa tccttgtccg gaagtcaatt cttcaaacgc
    13921 agggacaaaa ccaccagaaa aagcagtgac accgactagc catatcagga tctttttcca
    13981 gtcacggcta aggttttgag aaaaaagggt ggaccagcgt gaaaatttat cagtcatttc
    14041 gcttcctcct catccttcat aatgtctcat gaataaatcc tcaagagtcg gtggaataga
    14101 ttcaaatttt ttcacaccta atttggacgc ttcagctaaa atcacatcga aagcttcgtt
    14161 atcagcagca aattgagcga ggtgaccttt ttgcacaaag tcatggacac cgttcaattg
    14221 tgctaagtga ctattctctg cttctgtttc tatagtgatg gtcgaacgag tcaaatgacg
    14281 caattcatcg agtgttcctt cttcatcgag tgttccttcg cgtatgatca cgactttatc
    14341 cgctagtcgc tcgacttcac tgagaatatg agaagaaagt aggatggatt tgccctgttc
    14401 tttcaatttt tcaacttctt cttggaaaat cgtttccatc aaaggatcta aaccagaagt
    14461 aggctcatca aataaataga gatccgaatc aacagccaaa gcagcaatca agcccacttt
    14521 ttggcgattt ccttttgaat agttttttgc ttttttctta ggatcaagag agaaacgttg
    14581 aatcagttca tctttttttt gagcactccc ttttccatga agcttcatga agagatcgat
    14641 gatcgctcct ccagtcaaat ttccccacaa agagatatcg cctggaacat aagaaagacg
    14701 ttcatggata gataaactat cttcccatac atcttgccca aaaatcgttg ccgttccgcc
    14761 gtcttttttt ataatcccta aaagtgtccg aatcgtcgtg gattttccag aaccatttgg
    14821 tccgataaat ccaacgactt cacctgaatg tacggtgaaa gagacatctt ttaaagcttc
    14881 gaaacgacca aagctctttt tcaaatgtgt gactttgacg atttctgcca taaaaacccc
    14941 actccttttt ctaaaaatat atactaaaca agattaaata gtttaataaa aaggttttat
    15001 acc
//
LOCUS       DACEMO010000055        14995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEMO010000055 DACEMO010000000
VERSION     DACEMO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.056"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IW728_001284"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IW728_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1024654.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IW728_001285"
     CDS             complement(322..891)
                     /locus_tag="IW728_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1024655.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IW728_001286"
     CDS             1077..1979
                     /locus_tag="IW728_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1024656.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IW728_001287"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IW728_001287"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1024657.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IW728_001288"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IW728_001288"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1024658.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IW728_001289"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IW728_001289"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1024659.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IW728_001290"
     CDS             8547..8732
                     /locus_tag="IW728_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1024660.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IW728_001291"
     CDS             complement(8794..9990)
                     /locus_tag="IW728_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1024661.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IW728_001292"
     CDS             complement(10160..11596)
                     /locus_tag="IW728_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1024662.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IW728_001293"
     CDS             11889..12698
                     /locus_tag="IW728_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1024663.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..14881)
                     /locus_tag="IW728_001294"
     CDS             complement(12884..14881)
                     /locus_tag="IW728_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1024664.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4377 a         3178 c         2661 g         4779 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaa
//
LOCUS       DACEMO010000056        14953 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEMO010000056 DACEMO010000000
VERSION     DACEMO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.057"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            181..2652
                     /locus_tag="IW728_001295"
     CDS             181..2652
                     /locus_tag="IW728_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1024665.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2755..4491)
                     /locus_tag="IW728_001296"
     CDS             complement(2755..4491)
                     /locus_tag="IW728_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1024666.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4488..5192)
                     /locus_tag="IW728_001297"
     CDS             complement(4488..5192)
                     /locus_tag="IW728_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1024667.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5323..5826
                     /locus_tag="IW728_001298"
     CDS             5323..5826
                     /locus_tag="IW728_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1024668.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5786..6124
                     /locus_tag="IW728_001299"
     CDS             5786..6124
                     /locus_tag="IW728_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1024669.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6145..7563
                     /gene="ffh"
                     /locus_tag="IW728_001300"
     CDS             6145..7563
                     /gene="ffh"
                     /locus_tag="IW728_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1024670.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7675..8253
                     /locus_tag="IW728_001301"
     CDS             7675..8253
                     /locus_tag="IW728_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1024671.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8257..8778
                     /locus_tag="IW728_001302"
     CDS             8257..8778
                     /locus_tag="IW728_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1024672.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8857..9411)
                     /locus_tag="IW728_001303"
     CDS             complement(8857..9411)
                     /locus_tag="IW728_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1024673.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9664..9939
                     /gene="rpsP"
                     /locus_tag="IW728_001304"
     CDS             9664..9939
                     /gene="rpsP"
                     /locus_tag="IW728_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1024674.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9951..10202
                     /locus_tag="IW728_001305"
     CDS             9951..10202
                     /locus_tag="IW728_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1024675.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10327..11118
                     /locus_tag="IW728_001306"
     CDS             10327..11118
                     /locus_tag="IW728_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024676.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11288..11809
                     /gene="rimM"
                     /locus_tag="IW728_001307"
     CDS             11288..11809
                     /gene="rimM"
                     /locus_tag="IW728_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1024677.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11799..12560
                     /gene="trmD"
                     /locus_tag="IW728_001308"
     CDS             11799..12560
                     /gene="trmD"
                     /locus_tag="IW728_001308"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1024678.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12696..13043
                     /gene="rplS"
                     /locus_tag="IW728_001309"
     CDS             12696..13043
                     /gene="rplS"
                     /locus_tag="IW728_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1024679.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13416..14078
                     /locus_tag="IW728_001310"
     CDS             13416..14078
                     /locus_tag="IW728_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024680.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14157..>14953
                     /locus_tag="IW728_001311"
     CDS             14157..>14953
                     /locus_tag="IW728_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HAQ1024681.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKP"
BASE COUNT         5006 a         2711 c         3074 g         4162 t
ORIGIN      
        1 aacaccgttt attcgaaaaa aagagctacg acatagaact gtcgtagctc tttttttgct
       61 atatgacagg aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt
      121 ctatactgga taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac
      181 atgaagaata aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat
      241 aaagtcatcc gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa
      301 cgaatagaaa aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa
      361 atcgtattgg aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg
      421 ctttgtttag tagaatccta taagaacttg acttttttac cagttatcat agccaataca
      481 ggaatcgcca tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta
      541 aatacagcaa aagtcaatgt catacgggac gggatagaac aaacaataga tatagaagac
      601 cttgtaatag atgacattgt tattttagag actggacatc aaattccagc agatgcagag
      661 gtcgtggaag gaaaactgca agttaatgag gctttattga caggcgaagc agatgaaata
      721 accaaagaaa taggcgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac
      781 gcccgattag acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag
      841 acaatgggag aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg
      901 attgggatca tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat
      961 ggaaacacga tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct
     1021 gaaggactgt acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag
     1081 caaagagttc ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt
     1141 tgtgtagata aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa agtgatcgtc
     1201 tctaaaaaac aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat
     1261 gcaaaagcga tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa
     1321 accactgatc aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt
     1381 gtagcgttgg cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa
     1441 ttgccggaat atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc
     1501 tttggtgtgt acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta
     1561 ggctatattc tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc
     1621 aaaaaacagc aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat
     1681 gtagctgtcc aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca
     1741 gaagaagaat atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa
     1801 caaaagaaaa tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat
     1861 ggcgtgaatg atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga
     1921 aatgaagccg ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg
     1981 ccagaagttg ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg
     2041 tttttagtga agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg
     2101 acctatcctt tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca
     2161 tctttcttgc ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat
     2221 gtgatggaaa aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg
     2281 atctgcgggg tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt
     2341 ttgattgcag tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga
     2401 agtcagatca ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag
     2461 ctgttttcaa tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc
     2521 tcggcagatt cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa
     2581 aatgaaaaag agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa
     2641 cgagaaaatt gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc
     2701 tttcgaagca ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat
     2761 tttcggaatg gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt
     2821 accattaagg agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga
     2881 atgtcggctt cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc
     2941 aatgtcgata cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata
     3001 gcggataata atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt
     3061 gataattgga taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg
     3121 aatcgtcaac cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata
     3181 aggttctgta taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat
     3241 gatttcccga atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga
     3301 agtcagagtt tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag
     3361 tgaagtcaca ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt
     3421 ttctaaacgt cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc
     3481 tgaattagta aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat
     3541 ctcctgatgg atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg
     3601 ctcttgaatg acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag
     3661 ctgcatcttt ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc
     3721 aatcatcagt tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata
     3781 catcttactc atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc
     3841 gggtgtttgc atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg
     3901 cagcacggtt ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag
     3961 aacaaggaag aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg
     4021 taaaaaaccg cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt
     4081 gactggtaat gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct
     4141 acctaggttc ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt
     4201 caatgtttca ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg
     4261 ttcgttcgac tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt
     4321 ttcgacatct aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg
     4381 ttccacgatc tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact
     4441 aactaaaaga ataagaaaga agagacgttc ataaattttt ttccatttca tcgttttggt
     4501 tcctgaaact tatagccaaa tcctctaata gtcaccaagt actttggatg ttttggatct
     4561 cgctcgattt tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg
     4621 aagtcaaaat tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt
     4681 ttcatgaaat aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct
     4741 acggtcactt gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc
     4801 tcagcttcat cttttgattg cttacgaggt tcaatccgtc tgaatattgc tttcatacgg
     4861 gctaatactt cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg
     4921 ccaatgatcc gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt
     4981 ttttcttgac gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct
     5041 aaaatgatga aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg
     5101 gcactggtta ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa
     5161 ggttcatcat caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc
     5221 tgtttatatt ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta
     5281 taataggaag gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca
     5341 tgtttaggcg gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg
     5401 gaactagttg aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca
     5461 attcctagag atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa
     5521 gcaaaagtat ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc
     5581 aaagcctatc aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc
     5641 ccttcatgcg gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt
     5701 gccggagtgg ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa
     5761 tggctgaaaa ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct
     5821 ctttgaattt tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta
     5881 tgcagatgat ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt
     5941 atatgacaat atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct
     6001 tttttctaat tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga
     6061 taagtacccg gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga
     6121 ataaataaaa ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag
     6181 gcgatgggaa aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg
     6241 cgggaaatcc gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt
     6301 actaagcgcg tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca
     6361 caacaaatcg taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca
     6421 gagttaaata aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca
     6481 ggtaaaacga cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt
     6541 ccgctcctaa tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta
     6601 ggtcaacagc tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc
     6661 gtccgtcaag ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg
     6721 gcaggacgtc tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg
     6781 acgcaaccaa atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac
     6841 gtagctgata gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat
     6901 ggggatactc gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa
     6961 ttcatcggtt ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct
     7021 agccgtatcc ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac
     7081 gacgagaaaa aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat
     7141 gactttattg aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa
     7201 atgatcccag gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat
     7261 gttgaacgca aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat
     7321 ctgttgaacc caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa
     7381 gtgaatcgaa tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa
     7441 ggggatatga acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta
     7501 ggcaaaatgg cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa
     7561 taagatgaaa aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat
     7621 cagcaatttt tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga
     7681 tgtccgtgga gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt
     7741 cctgaatatg atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga
     7801 ttgagctggc agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc
     7861 gatatcaaaa aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa
     7921 ggaattattc gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg
     7981 aaaattcaag aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa
     8041 atcgttgatt cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat
     8101 gaattagcaa aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat
     8161 gcttttttag aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa
     8221 gaggtcgtac gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt
     8281 gctataagtt tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac
     8341 actccaacag gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc
     8401 atgggcagaa aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca
     8461 gagaaaaaga atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc
     8521 gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc
     8581 tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa
     8641 tggtatatcc agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga
     8701 acatatgaag cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg
     8761 cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc
     8821 tgtacatagt tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa
     8881 atctagatac gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc
     8941 attttcatca attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg
     9001 cacattcaaa aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc
     9061 acctggtgat gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg
     9121 tcttgagcct acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg
     9181 agtaacgaaa acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc
     9241 aggagcctgt tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac
     9301 aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt
     9361 gtaagaatct tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt
     9421 cctccttcaa actatctata ataatcttac aaaagataag taactggagc aaataaaaac
     9481 acttactgac aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta
     9541 aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta
     9601 cagggtaaaa aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa
     9661 ataatggcag ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt
     9721 atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact
     9781 tacaatcctt tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg
     9841 ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt
     9901 atgcaaaaac accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt
     9961 taagcgactt agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt
    10021 tagacgtcgt agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata
    10081 ttggccgtat cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg
    10141 gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt
    10201 aaataaacgt gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga
    10261 agaaatgatt atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa
    10321 cagccaatga gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt
    10381 tcggttactc aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc
    10441 agtccagaaa aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca
    10501 aataatgaga aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg
    10561 cctctagatg taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa
    10621 ggttattcta ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg
    10681 aatcaagaaa cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa
    10741 acaatccaag ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt
    10801 tctcgaatca cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa
    10861 ggaaactggc gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta
    10921 gctaaagaaa tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt
    10981 aaaattactg ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa
    11041 ccgaaaaatg cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt
    11101 tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca
    11161 caggaagtaa tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa
    11221 agaaaactag aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg
    11281 atataagttg actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg
    11341 cgaagtccgt gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt
    11401 gctaacacta ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa
    11461 acataaaaat tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa
    11521 atatcgggat ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga
    11581 attctattac catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa
    11641 agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa
    11701 agctgatata ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg
    11761 agtggtccgt gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt
    11821 tatttccaag aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata
    11881 aaggattatt agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga
    11941 cagtagatga ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct
    12001 atgataacat ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc
    12061 ttgacccagc tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac
    12121 acttagtatt catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga
    12181 cagatgaagt ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca
    12241 tgatcgatgc aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa
    12301 cggattctta ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata
    12361 aagggatgaa agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc
    12421 aattaaaaga atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg
    12481 aatggacacc tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa
    12541 cagcagatga agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt
    12601 tagtatttta tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag
    12661 ctcataagaa tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa
    12721 caaaagaaca attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc
    12781 acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca
    12841 aacgccgcgg tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg
    12901 gcgttgaacg tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct
    12961 acggtaaagt acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc
    13021 gtatcaaaga aatcagaaga taaacacgga aaacttcctt ggaaatctga aatttgtgaa
    13081 aagtccttgt atatcaaggg ctttttcttt tttctctatc acagttttaa gccatttttg
    13141 gctgttttgg accatttttc aatcatattc ccttaaaaat tgtcctagat tgtccgacat
    13201 tgactatcag tgtcgtcaaa atagggcaaa atttagtagt cagcttgacg acaaatataa
    13261 tacgaaaaat accaaaacat ttaccgaatt tagcaacttt tgaacccaag tttctcaaag
    13321 ggtcagctat tagcaaaaaa gacaaaacat gtacttttct actaaaaagt tgatgtgata
    13381 ggcttctatc taaataacta ttaggagttt ttatcgtgaa gcaaaatgat ggacgtatca
    13441 tttggaaaaa tcaaagtgag ttaaaactga ttttaacaat taatgaattc atcgaaaaac
    13501 acggaattac atctagccgt caataccaga aaaagctatc agaaaatcca aattctgcac
    13561 caagtatgtg gttcattaac aaaaaatatg gatcatggga gaatttattg attagtatag
    13621 gacgagaaaa tactgggtat ggaaaatggg ctagaatgtc agaacaagaa ttattggaaa
    13681 tagtagaagc ctttattaag tgcgagaaga taacctctca acgaatgtac gaacaaaaat
    13741 cagtagggaa gaatattcct tcacttagta ccattaaaaa gatgttagga gatatacgtc
    13801 cactttttaa agaaaaaaat gatggatctc gatttactga tttcgaactg ctgttagaac
    13861 taaaaaatga aataatccga ttaaaactac aggatgattt atcaatgaca aagtttcgaa
    13921 aactagttca atccccaaaa ctaccatcag ttgatacaat tatgaaaaga acgaacaaaa
    13981 attgggagga attgatggca gagattggat ttgattatcg aagaatcaaa atttataagc
    14041 agagaaacaa cctatccaag acaaagaaaa ctaaatagta tcactctaaa tcagacgttc
    14101 aaaattaaaa tttggacgtc ttttttatta cccaaaaacg aaaggagatt tattttatga
    14161 aaaagccaag atttaagaat tggcgtttgc tcgcaacact gagtctatta tgccaaacaa
    14221 ttggtggatc gcttggccca acgattgcct ttgctgatga aattactcat ccacaaacgg
    14281 taactgtgga attggatttg gctcaccagt atgctgtcga aggtacattt agcgatggtc
    14341 gtcccatgtc agaagttacc gttccgcact atgcagttta caatggtgtg aagcaagata
    14401 ttttctgtat tgagccaggg gtgccgattt acaatgagtt cactcctggt tatgagaaaa
    14461 atccattgcc tgatatgtcg gagaaagcga aattagtttc cgttttatgg aaggaagctg
    14521 gtacggatgt ggatacacat attgtggccc agaagatgat ttggcaagaa gttaatggct
    14581 acacgcttca ctcaattaaa cgttctgatg gtagtgcagt aaatatcgca gccattgagt
    14641 caaaaatcaa tcaagccatt gcggattatc aaaagaaacc aagcttccat aactccacag
    14701 tgaaaaccgt gttaggtcaa tcgactactg tgacagatac gaacggcttg aatttatcgg
    14761 agtttgatga agttgtcgag aatatcgcaa atattgatta tcgtgtgaac ggcaatcaac
    14821 tagtgattac tccaaatgcc aattcaaaag agagtggtga attaacccta aagaagtcag
    14881 ctaatacagg aacaccggta gcttataaaa tggctggtca acaaactttg atggcggggg
    14941 caattgataa gcc
//
LOCUS       DACEMO010000057        14763 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEMO010000057 DACEMO010000000
VERSION     DACEMO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.058"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..1004)
                     /locus_tag="IW728_001312"
     CDS             complement(93..1004)
                     /locus_tag="IW728_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1024682.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1405..1983)
                     /locus_tag="IW728_001313"
     CDS             complement(1405..1983)
                     /locus_tag="IW728_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1024683.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(2003..2671)
                     /locus_tag="IW728_001314"
     CDS             complement(2003..2671)
                     /locus_tag="IW728_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024684.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(2686..3756)
                     /locus_tag="IW728_001315"
     CDS             complement(2686..3756)
                     /locus_tag="IW728_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024685.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(3927..5291)
                     /locus_tag="IW728_001316"
     CDS             complement(3927..5291)
                     /locus_tag="IW728_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1024686.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(5288..6847)
                     /locus_tag="IW728_001317"
     CDS             complement(5288..6847)
                     /locus_tag="IW728_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1024687.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(6903..9383)
                     /locus_tag="IW728_001318"
     CDS             complement(6903..9383)
                     /locus_tag="IW728_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024688.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(9367..9702)
                     /locus_tag="IW728_001319"
     CDS             complement(9367..9702)
                     /locus_tag="IW728_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024689.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(9716..10750)
                     /locus_tag="IW728_001320"
     CDS             complement(9716..10750)
                     /locus_tag="IW728_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1024690.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(10840..11520)
                     /locus_tag="IW728_001321"
     CDS             complement(10840..11520)
                     /locus_tag="IW728_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1024691.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(11690..12649)
                     /locus_tag="IW728_001322"
     CDS             complement(11690..12649)
                     /locus_tag="IW728_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1024692.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(12870..13973)
                     /locus_tag="IW728_001323"
     CDS             complement(12870..13973)
                     /locus_tag="IW728_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1024693.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(14195..14665)
                     /locus_tag="IW728_001324"
     CDS             complement(14195..14665)
                     /locus_tag="IW728_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1024694.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
BASE COUNT         4182 a         3029 c         2545 g         5007 t
ORIGIN      
        1 cttaaacggt gtttaaaagc ttgcatctgc gataataagc ccggccaaac gaaaaatatg
       61 aggagctatt tttgtttggc tgggcttatt tattactgaa aataagtggt caatgcttgt
      121 gtcaaacttg cagcaatctg ctgctgataa ctgtctgtta ctaattcctt taaatcacta
      181 tcattattca tataacccat ctcgattaaa atagaaggtt gaagattttc tcgaagaact
      241 tgatagttcc cagtggagat tcctcttgaa tccagaggca gatcagtgaa ggagtcagca
      301 attgtctgtg ctaatgtttc ctcttgtcca tagtaataat atgtcgtgat tccagttgct
      361 tcattcgcat attccgtaga gtctgcgtgt aaactgataa agacatctgc ctttttttct
      421 tcactgtagt cgcatatatc tccaagctgt actaattcat ctgtagtatg ggtcaaataa
      481 accgttgctc cagcatcctc tagcgccttt tttactttta tggctgtact taatgttata
      541 tctttttctt ctgtttgttc atcattgctt aatgccccag tatcatttcc gccatgccca
      601 ggatctaata caatcactgc atcagatagc tcttccggtt ttgtttgtat attctttgtg
      661 actttggttc catcttcgag ggctaaatat ccagtatagc catcatttgt cttgatctga
      721 taagcatttt ttgtttcaga aagaaactcc gccagctctc cgccgtttat agttgcaagt
      781 ggggtagtta gcgagtcatc tgtatatagt gtacggtcat tttttccgat tttgattaat
      841 gtctcttgtt ctgtatcatc aaatgattcc tttgtctttg taaccgtact gttcgtactg
      901 ctgaccgttt gtccaactat ctttttttga ccaaaaacat ctatttgaaa acagataata
      961 atgataatga ttgcaaatac aaaagctcca attatttttt tcattcttga ctctcacctc
     1021 gtagatccag actaagggcc aaaacttaaa atcagcttaa gtaagcaaaa aaggggctgt
     1081 aaaagatata accgtagaaa ataacattac tgctaaaatg atttttttca tttttggcat
     1141 atcctgaaat ctccttttga ctattcgtgg tgattcatct acccaataaa tagtttcctt
     1201 cattctacaa acaattaatt tcttcgttat taaattaaaa cacttcctgg aacaccgaca
     1261 taataaataa aaatataccc tgataatcat gagagaataa caaatttgta tttatgaaaa
     1321 actacctata taaagaagca agatataacg atcttcgtca tatcttgctt cttcacctaa
     1381 ctattttctc cctatataac agctttacgc ttctcctaat acaccattca aatataaatc
     1441 gatcaattgc gccacattta gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg
     1501 tgctaacact aagaaaaaat gccttgctgc gatttctttt gaaatcccat ttcttagcac
     1561 agattggttt gctgaaaaga tttcttcaaa aggttcacga taatttttct tccatagaaa
     1621 gaaaagatga tctcttgttt cctttgaaag attgctttca agatcgtgaa gcatcagaaa
     1681 caaatcgacc agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa
     1741 tcccgtttcg cttgtttgaa cgatttctct caaagctttt ccaacatctt ttaagtggtt
     1801 ttctaaaact gccgtatata gtttttcttt atcaccgaaa taatgataga gattgggctg
     1861 ggtgatccca actttttgtg cgatttctct tgttgaagtg ccgagatatc ccttggacat
     1921 aaacaagtca gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt atcgatggac
     1981 catttttcca cctgcttttt ggttattctt tttctttcaa tacaccatct tccatcataa
     2041 atattctatc gcatttgtca atcagccgtg tatcgtgagt aaccatgatc gttgctttat
     2101 ttttgtcttt tgattctttt gccaagatat cgactacttc tcgtgccttt tctgaatcca
     2161 aactagcagt tggttcatct gctagaataa tggacggatc attgtacaat gcacgagcga
     2221 ttgcgaccct ttgccgttct ccaccagata gttcttctgg atacttgttg atcaacttat
     2281 ccacgccaag ctgctcaaat aattcgtttg cttctttttg acgatgttca tgtttaatct
     2341 tatctactaa taataactgc ttttttactg tcagaaaagg aaccagattg gaagcttgca
     2401 aaataaagcc aatttcttta aaacgtagtt taacgcgttc gctttctttt tgattactga
     2461 attcttgatc attgataaat acagagccac tgctaggcgt ctgcaatccg cctgccaaag
     2521 tcaaaaaagt acttttacct gaaccagaag gaccgatgat tgcaacaaat tcccctttat
     2581 cgatactcat ggttgtttct ttcaatgctt taactacttg gtcgccatct tggaaacttt
     2641 tttctacttc tttaaattct attgctttca tgttcttcct ccttcttatc caattgcctt
     2701 caacggatcg atttttacga ttgtccgaac agaaaatagt gcgcctaaca cagctactac
     2761 taacatcaac aagctaatga cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc
     2821 aggtagaaca agggatgttc ccactgttcc taatagacca agaagaatac caacaaatga
     2881 taaaatgaat gtttgtgcca ctacagaacg cgcaataaat cctcctgtga tcccttgtgc
     2941 tttcattaca ccaaagatat taatcttttg catcgtcagt acataaatga aaatcccgat
     3001 cacaatagcc gcaataacga tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc
     3061 attatatcca ggtaattcat tgatgaattt agaaattttg atctgttcta aatcttcagg
     3121 taaattactg attttacctc tgacaactat tgcgttaatc cgtgcatttt cacttgtatc
     3181 ttctttttcg aagcggattt cttggtaagc attgatggtt gtatagagaa ctggtgagac
     3241 attgaatttg gcatgatccg taaagccagt tagtttaaac gtcttgtcac taccagataa
     3301 cttaactgta tctcctatgg ctagaccata ttcttctttc aaactaatat cgcctactgc
     3361 ctcatcatcg ttatcaaaag cttttccttc aacaagattg ggcatgatga attgattttt
     3421 atcgataccg aagaagctaa cattgatttt tccttcttct gtactgtctt tactagtcac
     3481 gactccaggt gtttgggcaa gatacgccac tttcccacct tcgacttctt ctgctatctt
     3541 cttagtaatc atggacatcc ctagatttga atttgcatct ttcgaaagca caatcgaatc
     3601 tgcctcccac ttgtcgactg ctgttcgatt atcttgagct aagccataag ctaacccagt
     3661 caaaaaaaat accaggtagg cgatcaaaaa catcacccct gcaactaacg cataacgtaa
     3721 ctttgaatgc ataatttcat ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc
     3781 attttataca acaattttat cacttgataa atttttggcg aatattatgc tcaaaaaatt
     3841 cttccgctat ttcattatat gaatttttta ttctcgattg attaatattt attatatccc
     3901 cacacaataa aagaccgtga tatttctcac agtcttttat ctactctatt gctatttgct
     3961 ctcttagcta gtactttaaa agctaactca ttgaggtagt gaaaacgaga ggtgtattca
     4021 gacagaaatc caaaatcctc aactgctagc tgtggaatcg tagttttgtg tttcacgata
     4081 tccgcaagca ggttcaaatc tgataataag aattcttggc ttatcagctg tccgccaatt
     4141 atttctaatg tatccggatg gtagatcagc tttgcattta ctgttttgtt ttcttcgtcg
     4201 tattgtggaa atagatcaaa ttctcctgaa cacgaaccgg tattttgttc caagaatggc
     4261 gcttcatctt ctgtcagccc tacagttcct aaaaagtagt ctataatgac tgctgctgtt
     4321 atcttttgtc tgaggtcgta agcggttggc tgaccactca aagttagcgc tactgctctt
     4381 gccatatgta cagctcgagt aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa
     4441 ctgactggga cgatatctcc aatcgcatag atgtccggat cacttgtctg cagatattca
     4501 ttgactatca cagtctcatc ctcatacaaa gccacttttt cttttaacaa tgtcgtatta
     4561 ggacgagttt gggcagaaaa aatgaccata tctgccttta ttgtttgaga cagaagttgt
     4621 acggatacta ctttctcttc tgattttttc caatcgatca agaattcatt tggataaaaa
     4681 tttatttgct tagtttgcag ctgattttga aaactgttga tcatatcctt atcgaaatat
     4741 cttgataaaa cattcggcat ataatcaact agatgaataa tgtatccttt atttctcaaa
     4801 gcatctgcta attccaatcc aatgtaacca gcaccgaaga tcactacttc ttttgcttct
     4861 tttaaagcgg caagtacttg cttagattgc actagatttt tataggtgta gacattttca
     4921 gcttcaattt gtttatacaa ggtgacagga gaggaacctg tcgctacgat taatttatca
     4981 taggttatgg tctcgttttt tccttctttg gatgcgagga tgagctgatg tttgtctgga
     5041 tcaagttcag ttactctcca ttcggtgatc agttttgccc cactttcttg aagagtccgc
     5101 atgttatggg caacttcagc taaactacgt atctttcctt ttaaataaag gttgattcca
     5161 tttgaaacat agcctagtcc atctttatga tcttcatcga tcagtaaaac ttctgctttc
     5221 ggttgaagtt ttcttagatt tagggcagct gtgatgcctt catgggaagc accaatgatc
     5281 acgattttca tgttttattc atccttaaat tcaatttttc taaataacat tcttttaaaa
     5341 attggttcaa ataggggaaa tcataattat attcgttgct agtgaataca aaaactttag
     5401 cggacttctg tgacgggtac tctttcaatt ggctagtcaa aatgatatca tactgctttt
     5461 ctttctcgta ttcgactaaa tcatatttga tcgtattcag caattgttcc tggatgattt
     5521 ctttcacgat atccgccatt agcctttcat aaggaagatc ataagcaatt tgcagacgta
     5581 attctaactg cttgatcagc gtccgtaaaa tagatgcata gcgagcaaat aattcaaagc
     5641 gggcattttc ctcgtctact tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc
     5701 tttcacataa aaccaggaat tgtttatctt ctacttcagt taaccgctgt tttaatcctt
     5761 cttggccaaa aatattcatc catcctctaa aataggcaaa atgagcgtga ttttgcatca
     5821 ctgtcaacgc aatcttttgc gaaatctctt ctggaaaaac ataaaaagag aaatattctt
     5881 ctatcgattg tttaaacgtt cgattcaatt gagctactac gtcgtctttg tatttttcgc
     5941 ttatcaaaaa ctcctggaca tgcgagttat gtgtatctaa tgtaaagtta ctgatcatga
     6001 aaagataaaa aaccaacgtt tcattttgat tccaaggaaa ggcatattga aagaagtaac
     6061 gggtcaaaaa tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa
     6121 gattgctata atctggtttg gattttttcg tacagcgcat tcgcttcttc ataatggaaa
     6181 gccatatttc aaatttcaat cgtccttctt ctgtaatagc aaaactaaac tctttttgca
     6241 gcagatctaa aaaagtgttt cctgataaat cactgtattc ttttttgttt tcaagatagg
     6301 gacgtccaaa ccaaaataat tggaaataaa aataacgaat ttgactttct ggaccggtca
     6361 gccgcccgct ttttattttc agatgaaact catttaaaga atcatttaat tccttcactc
     6421 tgcgagacaa agtagccgaa ctgatattaa gttccatgac aagtttctgc cgatcaaatg
     6481 agccattttc caacagataa agaagtatct gatattttgt agaatagcgc aagtaacaat
     6541 gataataaac atcgactgct gctatatttc catgtctgat taataaaata ttcccttctt
     6601 ggataacaat ttccaattgt ttgatatttt ccaagttagc ttgtaaataa aacagatctt
     6661 ctttcaaggt agcccgatca actgataagc gggtaagtac gttattaacg gaaacctctc
     6721 ctttttcctc taataaaagt tggaaaagtt tgatttgccg ttgttcattt ttttcaagca
     6781 aactgaaaag ttccaagtcc actccttcaa gcatttctga ttttaacagc agaagtttcc
     6841 ttctcatcct tgaaaaagag gatctgtgac agttatcaca gatccataca aattctaatc
     6901 acttagctgg ttggcccagc tgtcagttcc catgtgacca tgccttgata cgttgcgcca
     6961 ggttgagcat tcgtactgct atttacctct aagagtaaac catcggtatc gggaaagtca
     7021 acgagaacct ctcctttagc tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc
     7081 tgaatcaatt gctttgatcc agaatgattg aagtataatt ttgttccgct taattcttga
     7141 ccttgatcat tcttaaataa agaagtcaat gtacctgtca gttgccaagg agacgtgctt
     7201 ggaagccgag tatcccgcac atcaaaagaa aaatcgcctt ttgattcata ataacttgtc
     7261 ttcaacggtt tggggagatc gttaccaaat gtaaaatcat ctggtacatt aactaatgtt
     7321 aattcttgtt tggggacaag aattgtttta gcagatgttt ggacaactgg gttaggaagc
     7381 ttagcaccat ctgctaatgt aatattgctg gaaagtacag ctgcctttgt cagtatgctg
     7441 acggattgta aagaagtatc tacagtattt aattgatagt tgtaagtaag atgaatggta
     7501 tctcctattt tgctcaatag atctgctggg acagaaactc cttgttgaat gccggtatct
     7561 gcgatagttg taccatttaa ttttaaggaa ccattgatat aagtagtatt ttcaggaagt
     7621 gagatcgtga tttttttgtc ggttatcgca tccccagatt ttgttaacgt gaatgtcaca
     7681 ttttcttgaa tcgtatctcc tgatagagca gacaggggat ctcctgtctc aggttgtctg
     7741 ctaaggtttt ttagctgact atctgcactc acagatggaa aattagccac tgttatttga
     7801 ggaagagtgg tgattccagc ttgttcagac tgccatttgt acgcaaaaga cggatacatg
     7861 ttagtaagta catcgtccac caagcgataa tcaaaataat attgccctga agccaatgca
     7921 ggtagtgtag aagtagctgt atttacaccc ccgttatttg attggatcgt ttggaaaagt
     7981 tgatctgtcc atcctgtcgc taatttttgg gttcctttcg aataaagcct gtaatggatc
     8041 gaaccctttt ttagttcagt tgctgaccaa ttggccgtga cttggtacga tgcagaagaa
     8101 aggagctttc cccactgact atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc
     8161 ttgtactgct ccaatacctt taaagcaaca ggagaaagca atccgccttg cgcatctttc
     8221 ccactgtaca cgatattggc tgtctgattc aatccacttg tgcttgctgg agtatgagtc
     8281 agtattgctc cttgtgtaac gttttttcct gtaaaatagt tgactacgcg atcaaagcga
     8341 ctaaatggca actgatcaaa acgtccttca gtcagcccca tcgtcaatgt ccgactattg
     8401 gtagagcctg gaaggttctg gttatttcca tccacgtatt ctgtggtatc agcaaatgta
     8461 ttatattttc ctgttgaatc acttggtgag atgtaactat tccacgtatt tatctctgca
     8521 cttgcagaaa atgcatatgc tcctggattc aagtttccgc cagttaattt aggataaaat
     8581 ctacctgcag ctggagctaa aatcgaattg aacatacgat cttggataat attattcgaa
     8641 tcataaaaat caacagaaaa aacggccttt ttagacatag aaggttgtaa tttacccttg
     8701 gtcaaaattt tgtttggact cgtatcatat ttatctccgt tttctgtcat cttcgcgaca
     8761 ttagctgtaa catcaaattg aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata
     8821 atagcaataa tatctgtcaa tagagtattt gtatcccacg accccatccc aaaacgcaag
     8881 gtatggcttc ctgctgataa taaacttccg ctagtaacct tgatgggggc accggcaata
     8941 tacaagtagg cgttgttcca gtcaattgct tttaatacat cactgcttga tacatgttcc
     9001 ggcaaattga tgatcgccac aaatgcaaat ccttgtgcag tgatattcac caaatcaagt
     9061 acatttgtta gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc tttccaagga
     9121 tcatttgaac catcgttcaa ataagtagca gtcaatttag gcaattggtt agccccagtt
     9181 agttggacaa gcttcccgcc attgactgca ctacgtccac catttggagg tacgatattt
     9241 tcatacgtgt atggatcata agaaatggca ggaggcgttg ctccaacagc ttctgccgaa
     9301 ggtgaaaaga taaatatact taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat
     9361 gaaaatttat tttgtagcaa catgttcatc ctccttttcc tttcttctcc ataacaacaa
     9421 ccacgcaaga agccataatt ctataccaat gatcaaataa agaaaacgga tcttttgttc
     9481 attcgtctcc gggaatacgc catgttgatt agattcttta taaatcggta gctgtgtctg
     9541 ctgttttgat gcgccattga ctgaaccact tggatgcgga attggactta gatcataaaa
     9601 tgtaatcgta cctgaacttt tggtatctgc ttggactggc tcttgccagc tgaagaacag
     9661 caaacttgca ataatccctc ccaataagat cgatgttttc aacgaaatca cctcatcatg
     9721 ttgtttctcc ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca
     9781 ctagaactgc agcaaccaat cctgcataaa gccatgggaa agattgttct tgtactgcaa
     9841 aaacggaatt ttcatttact ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa
     9901 atcgccattc attactgtcg cctgattttg ctatgatttc tgcgcggtaa tcgccggctt
     9961 ttgctttatc accagctaaa tcaagattca agtgaaaatt cgaatttggt gcaactaaaa
    10021 atgcattaga cgtattttca atataaagct gattttcata aaagatcttg ctcgtaaata
    10081 tcaaattgct gataatccta ggctgaggat tttggaaacg aagctgaaca gttggctttg
    10141 cgttgacctc gtccaaatct cctgataaaa gtttcagatc cggttctatc gtctcttgac
    10201 tttcttgtaa aacgactgcg agattataag caatacgatt tttgatttgt gctccttctg
    10261 tagcattttc tgtttcagct tcttctttag gtgaaacatg aatacccgct aatacacgtc
    10321 ccttaaagct ttttgctggg attttcactt ctataggtac agttgtttct gcatgtgctg
    10381 atacattgat ttcctttttc gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct
    10441 catctaacgt tgggtttttt tgactataat cgatcgttcc accatcactc gttaccgccg
    10501 gattgacaga gacctcaaat gttcgttctt catctgaaga attgctgata attacttcta
    10561 gtgtctgatt ttgttccggt gccactaata aatcaaaata accgattgct ttatctattt
    10621 ggttctctgg aaataccggt ttgattccaa agtctcctgc tgaatccttt gcttggctga
    10681 ttgacggaaa aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac
    10741 tttgattcat tgtccaccac atcctttcaa caacaactgc ggtaaaaaat ccttcaccta
    10801 tagtattttc accgcagttt gttgaattta tttcatactc tatgctactg ggccagccac
    10861 gaggttccaa gtcaaatcag ccgtgaatgt tccagctgct gctttcggca ctttcagtgt
    10921 gatatttgct ggatcataaa cagaaagcca tgttcccaga ccttgatctt ttgctgctgt
    10981 aaatattggt gtcgctgcac ttgctttact caacccagtt acagtttgac tagttggtcc
    11041 agtggattcg ttatctgacg tacttttaac agtggatgtt ccaaaatcta attcagcgcc
    11101 ttgcaaaact tgtgaaccgt ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg
    11161 tgcttggcca tctgcaccta cacctcgacg atctgatact tggagataag gtgtatcgtt
    11221 ttttgaagcg tctacttgat acgtttttgt tccgctttca atctcgtgtg taccaaatgg
    11281 caattcggga actacatcca gtgttaaagg acctgtctgg ccagttgctg gattttccgg
    11341 atctgctggc tctactggtt ttgaaggatc agtagggtta agaggctcca cggtatttgt
    11401 tggttcagta aaggtaatat ctccttttga agtcgcttga ccctcagcaa aagctggtgt
    11461 tgccaagccg cctagaagaa gtgttgaaag taataatgtc ccaatcatgt tctttttcat
    11521 aataaatcac tcctttttct tagtgaccta agtatagtag agacagtatg caaaaaatcc
    11581 tcatgaattc atccgcttac ttgaaattat gacccttttt gtataaaaga gaaaaaaaca
    11641 ataaaatcgt ttacatgaat attcttcctt ttttattaag tttcgtttgt caaattaagt
    11701 tagacaaatc atcttttgtg acgtaactca aaaatacttc cattggtgtc cgatagtgta
    11761 atgattttct aggaatatga tttcgtttcg atgccaccga ttggataaag gtttcagata
    11821 caccattaaa atccatagct ttatacaaac catctttacg taataagcca tttgaatgtt
    11881 cattcaatcc tcgttgagaa ggtgttcctg gatccgcaaa ataaattgaa atatcgttgg
    11941 tattactaat ctttttccaa tttgagaatt cttttccaca atcaaatgtg atggatttaa
    12001 ataagttttt tgggacacaa cgaaaccatt gattcagcct attttcaatg tctttagcct
    12061 gtctgccttc gggtttcaag gtaatgatga ctttagatag ccgttcaact aatgtgatga
    12121 ccgcgctttt atgttttcca cccacgatag tgtcaccttc caggtgacca aattcatgat
    12181 caaactgggt atagtctgtc tttctgtctc ggattgaccg gcgaaaccct tgttttcctc
    12241 ttttttcttt atgaccgttt ggttttcttt ttcccttcat tggtaaatcg tgagtgtcaa
    12301 aggttttatc tttaaacatt cgatacagtg tgcgtaccga acaagcaata ggaaactctg
    12361 ctcgaccaat aaggacatca ggagcccacc cttgaacaac ctttttttga atgtattcct
    12421 gttgctcatc aggtaaaaca atcggatgtc ttccgcagtt acgtttgttt ttcttatatt
    12481 gtttatagta gctcattgcg cttccaccag atttaagaaa acgatagacc ttataaatag
    12541 tttgtcgaga acgttgtaac agattggcta cttttgaaac aggttgtgat tgattgaaat
    12601 aagcttctat cattacgagt tcagttggtg taagatggtt ataggtcatt tgtactcact
    12661 ccttataatt ctttcgtcgg aactattttg agtgtagcac aaatgacttt ttttagttgt
    12721 ctagcttaat tttacaatcg gcgaagtatg aaaaaatatt tcaattttat tttttcgttt
    12781 caaattattc tattagtgac atccttgaac tataaaaata ggagtacggc atcatctgcc
    12841 gcactcctat ttttttcgtc atttgtttct taaataaaac gattctcacg caattttatt
    12901 tttttgatat cttctactgt ttgtgttcca gctaattgca tgaccatttc caattctgtt
    12961 ttaaagaaat caaacacttg atggactcct gtacttcctc ctaaagacaa tccataaata
    13021 acgggtctgc caatagcaac aagatctgca cctgatgcga ttgctttgaa aacatgctgt
    13081 ccgcgtcgta caccgctgtc aaacacgatt ggtacacgtt tatctactgc ttctgccaca
    13141 tattgcaacg aatcaaacgc tgctggacca ccatctagtt gacggccacc atgattagat
    13201 acccagattc cgccagcacc agattctaat gaacgataga catcttcttc tgactggaca
    13261 cctttcacat agactggcaa atcagaatga gcagcaataa attctacatc ttttggacta
    13321 agcttctgtt tggaagattt gtacacagca tccatcgttt ggcctactcc tgattgataa
    13381 gcctgaacga tcggcattgg cagtgggaag gtaaacccat ttctgcggtc tgtttcacgg
    13441 tttccgccaa ctgttgcatc cgctgtcaat acgattgctt ttgctccatt acgcttggcc
    13501 acttcaagaa tatccaaatt gatcccatca tctttgctca tataaaattg aaaccattgc
    13561 ggtgctttct ccccgcctgc ttctcttatc tcttctaatg tacaagaagc gtaagaactt
    13621 gctgtataga tcgtcccaaa atctgctaca ccttttgcag aagctttctc cgccttcaca
    13681 tgagctaagc catgagcagc aactggtgcc atgatgattg gtgcagtcag cgtttctttg
    13741 tcaaaatgtg tagttgtatc gggtaattct acatctctta atacatgagg aataatcagc
    13801 tgatgattga atgcccgttc attttcttga taagtaaata agtcgccagc accactgctg
    13861 atgtaaccat aaccaccagc tggaatcact tgtgaagcgg ctatttctaa atcttccata
    13921 ttgataaaat ccactattcc ttcatttgta cctgcttgat acgttttttc catttcaatg
    13981 ccttccctct ataaaaaatt gaatgaataa tatgatagcg tttacacact ttatattact
    14041 cttccgtatt gatacaggca aataatctgt ttacatttca gtcattccag ttgttactac
    14101 tctgaaagaa atgtttagaa cacaaataga taaaaaacga tgggattttg acaaaagtcg
    14161 tgtcaaagtc ccttttacga aatactgtgg atgcctattg aaataagtgc gggttgtttc
    14221 tttgtatttc aatcgtttct tgccattctt cgtttaattt tggattaccg ttttcttcct
    14281 tgaacgcata aggtcccaat ccaaaaagct cagttagtat ttgattcacg attccatata
    14341 gctcggtgtg atcggacaca tgattattga agatcatctc attatcacca cgtttgaatc
    14401 tcacattcgg gctctcatga tgaacctcta tgcagttttc cccttttttt aactgacgga
    14461 tttctgaaaa catctcataa atatcttctc caataaaacc ttcagcaagc gccagactgt
    14521 ccacaagacc gcctactgtc aactcagaga ttatcgcttt gttggcacca agctttaatg
    14581 tatagcttgt tgcttctact acgccgcatg cattgcaacc accttctgat agttcgcttt
    14641 ttctgatcaa ttgaacttct ttcattttct gttcctcctc ttagttttct acttatgtat
    14701 tttttatcac ctgacgtaag tgtattttat cgtcacgatc acgtgattta ttcttcagag
    14761 att
//
LOCUS       DACEMO010000058        14549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEMO010000058 DACEMO010000000
VERSION     DACEMO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.059"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            3..815
                     /locus_tag="IW728_001325"
     CDS             3..815
                     /locus_tag="IW728_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1024695.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="IW728_001326"
     CDS             812..1612
                     /locus_tag="IW728_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1024696.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="IW728_001327"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="IW728_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1024697.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="IW728_001328"
     CDS             3109..4818
                     /locus_tag="IW728_001328"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1024698.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9388
                     /locus_tag="IW728_001329"
     CDS             5030..9388
                     /locus_tag="IW728_001329"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1024699.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9533..10006
                     /gene="rimP"
                     /locus_tag="IW728_001330"
     CDS             9533..10006
                     /gene="rimP"
                     /locus_tag="IW728_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1024700.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10029..11204
                     /gene="nusA"
                     /locus_tag="IW728_001331"
     CDS             10029..11204
                     /gene="nusA"
                     /locus_tag="IW728_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1024701.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11226..11519
                     /locus_tag="IW728_001332"
     CDS             11226..11519
                     /locus_tag="IW728_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1024702.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11516..11827
                     /locus_tag="IW728_001333"
     CDS             11516..11827
                     /locus_tag="IW728_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1024703.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11840..14146
                     /gene="infB"
                     /locus_tag="IW728_001334"
     CDS             11840..14146
                     /gene="infB"
                     /locus_tag="IW728_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1024704.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14173..14520
                     /gene="rbfA"
                     /locus_tag="IW728_001335"
     CDS             14173..14520
                     /gene="rbfA"
                     /locus_tag="IW728_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1024705.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4958 a         2584 c         3238 g         3769 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5701 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5761 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5821 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     5881 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     5941 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6001 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6061 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6121 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6181 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6241 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6301 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6361 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6421 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6481 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6541 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6601 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6661 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6721 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6781 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6841 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     6901 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     6961 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7021 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7081 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7141 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7201 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7261 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7321 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7381 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7441 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7501 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7561 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7621 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7681 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7741 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7801 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7861 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     7921 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     7981 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8041 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8101 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8161 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8221 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8281 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8341 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8401 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8461 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8521 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8581 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8641 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8701 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8761 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8821 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     8881 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     8941 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9001 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9061 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9121 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9181 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9241 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9301 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9361 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9421 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9481 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9541 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9601 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9661 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9721 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9781 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9841 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
     9901 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
     9961 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10021 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10081 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10141 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10201 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10261 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10321 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10381 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10441 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10501 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10561 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10621 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10681 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10741 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10801 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10861 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    10921 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    10981 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11041 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11101 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11161 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11221 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11281 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11341 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11401 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11461 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11521 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11581 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11641 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11701 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11761 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11821 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    11881 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    11941 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12001 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12061 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12121 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12181 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12241 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12301 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12361 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12421 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12481 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12541 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12601 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12661 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12721 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12781 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12841 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    12901 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    12961 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13021 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13081 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13141 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13201 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13261 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13321 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13381 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13441 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13501 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13561 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13621 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13681 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13741 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13801 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13861 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    13921 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    13981 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14041 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14101 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14161 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14221 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14281 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14341 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14401 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14461 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14521 tcaaaaactc ccgcaaatcc tgtgaaatc
//
LOCUS       DACEMO010000059        14148 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEMO010000059 DACEMO010000000
VERSION     DACEMO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.060"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(35..1846)
                     /locus_tag="IW728_001336"
     CDS             complement(35..1846)
                     /locus_tag="IW728_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1024706.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(1843..2208)
                     /locus_tag="IW728_001337"
     CDS             complement(1843..2208)
                     /locus_tag="IW728_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1024707.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2371..2766)
                     /locus_tag="IW728_001338"
     CDS             complement(2371..2766)
                     /locus_tag="IW728_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1024708.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2784..3746)
                     /locus_tag="IW728_001339"
     CDS             complement(2784..3746)
                     /locus_tag="IW728_001339"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1024709.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3746..3958)
                     /locus_tag="IW728_001340"
     CDS             complement(3746..3958)
                     /locus_tag="IW728_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1024710.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(3979..4677)
                     /locus_tag="IW728_001341"
     CDS             complement(3979..4677)
                     /locus_tag="IW728_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1024711.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4697..5239)
                     /locus_tag="IW728_001342"
     CDS             complement(4697..5239)
                     /locus_tag="IW728_001342"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1024712.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5371..6375)
                     /locus_tag="IW728_001343"
     CDS             complement(5371..6375)
                     /locus_tag="IW728_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1024713.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6372..7361)
                     /locus_tag="IW728_001344"
     CDS             complement(6372..7361)
                     /locus_tag="IW728_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1024714.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7358..8164)
                     /locus_tag="IW728_001345"
     CDS             complement(7358..8164)
                     /locus_tag="IW728_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024715.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8330..9286
                     /locus_tag="IW728_001346"
     CDS             8330..9286
                     /locus_tag="IW728_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1024716.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9363..9881)
                     /locus_tag="IW728_001347"
     CDS             complement(9363..9881)
                     /locus_tag="IW728_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1024717.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9969..10118)
                     /gene="rpmG"
                     /locus_tag="IW728_001348"
     CDS             complement(9969..10118)
                     /gene="rpmG"
                     /locus_tag="IW728_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1024718.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10346..10792
                     /locus_tag="IW728_001349"
     CDS             10346..10792
                     /locus_tag="IW728_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1024719.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(10987..12882)
                     /gene="asnB"
                     /locus_tag="IW728_001350"
     CDS             complement(10987..12882)
                     /gene="asnB"
                     /locus_tag="IW728_001350"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1024720.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13207..>14148)
                     /locus_tag="IW728_001351"
     CDS             complement(13207..>14148)
                     /locus_tag="IW728_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1024721.1"
                     /translation="HYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKNLDKHYAMIGIA
                     AGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFIPWILGQCSTID
                     EAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHLYDNPVGVLTNN
                     PPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATF
                     TKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSCCNVDKGIYYYR
                     TYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         4397 a         2990 c         2380 g         4381 t
ORIGIN      
        1 tttctaaaaa acagataagt catcatgcgt atttttattt gtcagtcaat ataagttgtg
       61 ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct actgctgcag
      121 ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac tggatttccc
      181 ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct gaatgataac
      241 ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc aaatttttta
      301 tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc
      361 gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt tgctgaatat
      421 caaatggcgg ttcgatcaaa tgaaaaatac ccgcatctat cagtgctgct aattctttcg
      481 tccgaatgat tggcggaccg atcgttaaaa agctatttag atgagtaaac catccccata
      541 gtatttcttt gtattcctta gcagaaaaaa cgtgccattc aatagcctgc cggattggat
      601 cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt
      661 ccgcttcttt tatctgccct tcaataaata ttcggctagc ttctgaaaag ggctcattct
      721 gataggcaag aggagtttct aaatacgacc aatcccatct ataagggatt agtgctggat
      781 gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa tttttctttg
      841 gaatcggcaa gtgatattct tcgatgattt tcttgtaata aaacaactca acgtcttttt
      901 tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat tgctccaggt
      961 tttcatttgt tagtaaccgt ggccatgcca ttgctccgcc ttgcttttga tttcttcctc
     1021 gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc ttgcctgatg
     1081 gatgatagac cagttttccc ttttcgttct cgaagcgccc tcctcttgat actgtaaaca
     1141 atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt tctttaggcg
     1201 gtatcttcga aagatctaca tctgcaggat tagaaggaag ctgatacagt aaaacatttt
     1261 tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc cagtgaccgc
     1321 ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa agataaccat
     1381 cttttctttt gcttactttt tttactaaag aataaacaaa ctgaatagaa gctggaaatt
     1441 cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat cgagtagatt
     1501 gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt ttggttaccg
     1561 tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg ggtccctgtg
     1621 ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag
     1681 aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca ggaccattgg
     1741 gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga atcctttctg
     1801 ctacacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc atgcctccat
     1861 ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg caccataaac
     1921 aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag cttgcttctt
     1981 ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa atcgttctgg
     2041 tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca accacagatc
     2101 taactgcgct ttttcttttg aaattcctcg tggccagaat ctatccacga gtattctata
     2161 gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag ttgtttcctt
     2221 ctttctacct tttcgcttag cttatatatg tagataatag cacaaaataa aaaaaatgaa
     2281 cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat gattgattcg
     2341 gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga tttttccaga
     2401 ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt tacgtaattt
     2461 atttgctgta cggatgctca aacttttctt ttgatcgtaa atatagacag gctggtcttt
     2521 tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat
     2581 atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt ttctcattcc
     2641 ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgt ttggccatta ctcgtagata
     2701 gacttcccaa aggatgattg caagcacaat caatagcaaa atgatattaa ttacccataa
     2761 aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc agcaccaatg
     2821 acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct tacttgcggg
     2881 aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc tgctgccgat
     2941 actcctccac ctaacacgat acttgacgga tttaatgtat tccccaagtt tccgcaagct
     3001 aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc gtcttcttga
     3061 gccatttcaa agatatcttt acttgttact tcttctccgt tgtctagcat tgctttcagt
     3121 tttgaatccc ctgcgtattc ttctgccaag aaacgaccta ggcgtaccac acctgtagcg
     3181 ctagatacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc
     3241 acagtgatat gtccgatttc tcctccagct ccgctcacgc cgtgaatcaa atttccgcca
     3301 gcaacgatac ctccgccaac acctgttcct agagtaacga atacaacgtc tggatcgttt
     3361 tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcgtcatt atcgatagcg
     3421 aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc tgtccagttt
     3481 aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg ggtacccatg
     3541 ccgattccga taaagtcttc ggctttcata ttatataaat tcaaacgatg attgattgat
     3601 tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc gatgctccat
     3661 ttttgctgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa
     3721 tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt tcttcaattc
     3781 ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca atcaaacggc
     3841 tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg cgtgcaccca
     3901 catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag ctctccatct
     3961 ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca gtaaaataca
     4021 tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta tcgaatagct
     4081 tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa ttagcccacc
     4141 gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg tcaacacaat
     4201 aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc
     4261 taagataaac aacgcaccga acaaaccgaa tatcgaagta cttgcaccac cagacagaac
     4321 attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga acatataaat
     4381 caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa aatacagtat
     4441 cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg tcactaaacg
     4501 ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat aagggatctc
     4561 aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata
     4621 ggtccataac ggtcgtctta accatgcacg caattttatt tgctgttgat aattcatgaa
     4681 aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca aagctctccg
     4741 gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt ttatgaaaat
     4801 tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg gctctattgt
     4861 agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc ttctctgatt
     4921 gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc atttcataaa
     4981 acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt tctttttctg
     5041 ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc gtccctatca
     5101 cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc tgttcttttt
     5161 cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc cggcgtaatc
     5221 ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt ataggaaaaa
     5281 aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa gaagacctag
     5341 aaatgattgg ccttcctttt ttaatgaatt tcaactgttc agcgcgtgtt tttgtctgat
     5401 aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc ctgtaggtac
     5461 ttccccactt agtagaatca ctctagcaac catatctgca ctaagcagaa aaatggaacc
     5521 gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta gcttcgcagc
     5581 atgaggagct agtagtccta caaaagataa actacctgtc accgctacac taccgcttgc
     5641 caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa ggcctaatcc
     5701 ttgcgcgcgt tcttgaccaa atgatagcaa tacaatcctc tgtgcataaa gtcctcctag
     5761 cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc tcgcacccca
     5821 gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata attggatcgt
     5881 gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca ataagattcg
     5941 attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc aagcagctaa
     6001 aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt gcgaaaagaa
     6061 acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata tactagaatc
     6121 tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa gacaagctcc
     6181 tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca gcgccaaaga
     6241 agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc
     6301 aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat agaacgtcca
     6361 atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg aacgccaatc
     6421 aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct cgctgtaaaa
     6481 tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac ataacgatag
     6541 tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa tgaaacaggt
     6601 ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac aatcaaccgc
     6661 gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg aatcaaagat
     6721 attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg aaacaatact
     6781 gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc aagatcctgc
     6841 tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga actaatagct
     6901 acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg caaaaaacca
     6961 aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac agaaaaatca
     7021 gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc cgcgttgatt
     7081 cctagtaagc ctgaatctgc taaaggattt cttgtcactc cttgcatcaa tgcaccgctc
     7141 aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg aatatttcgt
     7201 atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca aaaatctgct
     7261 tttcccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa
     7321 cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc tcctgctctt
     7381 ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc gtgacaatcg
     7441 ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct tcaggtgtac
     7501 cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa cgtgaagcat
     7561 gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg atttcagtca
     7621 ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga
     7681 tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg gtttgtccac
     7741 cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca tccaacgccc
     7801 actcgattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga taagggtatc
     7861 gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta gtttgtgcta
     7921 ataatgcaat tttttttgca agttcttttg aactgtatgt agagatatca gagttattga
     7981 caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat aaagtagatt
     8041 ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc gtaacagaca
     8101 tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct cttaatctcg
     8161 tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc
     8221 gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt cgaaatatca
     8281 ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa tgaaattaaa
     8341 aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga gcgcttgctc
     8401 cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat ccaatagtac
     8461 tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc ccaaacggat
     8521 catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta tcgcccagtg
     8581 gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg atgtcccaac
     8641 gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt tgctgttgat
     8701 ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac
     8761 ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag atgtcggcaa
     8821 agctttggca aaagaaaaag aggcagaaaa gatcatttcc gattatcaaa aatccatcaa
     8881 gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag tgttgtgggt
     8941 gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa tcgtatacga
     9001 cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag ccacttctga
     9061 ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca tcatccttgt
     9121 gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc ttaaagcagt
     9181 gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata acggacctat
     9241 tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt aactaaaaaa
     9301 agcatcttca cgattagtcg tgatatgctt tttttcttat taacaaacta gacgcataga
     9361 ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg aaaacgatgg
     9421 gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc gaagtctgaa
     9481 aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag
     9541 atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga aacatagata
     9601 tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat tacttccgca
     9661 ttttctttcg tgcgaaaatt tggctgcgtt gtcaacacga tgatatggcg ataaggaagt
     9721 gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg atccattgta
     9781 tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc atttttgccg
     9841 atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc tccttatgat
     9901 tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa ttgagaactt
     9961 taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt
    10021 tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata gttacgttct
    10081 ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc catttttaca
    10141 agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt ctagattgct
    10201 agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac ggttttactt
    10261 ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat atagattatg
    10321 ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc ctgcatcaat
    10381 gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg cattattaga
    10441 tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa aaaaatggta
    10501 tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac
    10561 ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga
    10621 atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt
    10681 tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac
    10741 tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt gaacatatgt
    10801 aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct
    10861 ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg ttttttgaaa
    10921 ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat tgatatttga
    10981 ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt catctacaaa
    11041 gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt gctcttcttt
    11101 acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa aaaattcttt
    11161 tgcaaaatct gcttcgaaca atgctttgac ttgttcgtag ccgtgttctt ctttcaacca
    11221 agctttgatt ggcacaggga atcctaattt cacacgattc gaccattttt ccggaagatg
    11281 tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata aaaacttact
    11341 tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc ggacttccaa
    11401 agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca tccattgatg
    11461 aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt tctcgtaatg
    11521 aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta acacttctga
    11581 tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg caggagcaag
    11641 tgattcatat aaatggatcc gtccatggaa atttttcatt tttccgattt tacgtcctaa
    11701 attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac ggatgaattt
    11761 tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg ccccttctcc
    11821 agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca aaggtacgca
    11881 agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg ctttgaacga
    11941 catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg ttagttttct
    12001 ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag aatgatctgg
    12061 tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag aaccaacttc
    12121 tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga ttttttcgat
    12181 ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat attgctgaat
    12241 gtctattttt ccatctttat acgtataata atggccttca ggcaaacgat agacatcttt
    12301 gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt tcaacgcttc
    12361 tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa
    12421 cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt ggtctcttgc
    12481 accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc cgcggatctt
    12541 ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg tatctgcatg
    12601 tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt aattatagat
    12661 ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct gagtaccgcc
    12721 ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat cgatatattc
    12781 tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa taattgtttt
    12841 tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt cacatcctaa
    12901 tataaaatta ctgtttattt cattcatatg catcttatca tagaataaat agcggacgaa
    12961 tattttctct cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca
    13021 gaaaataaag tcccaatatc taatttacca tgttttcttt tttgaggcta ccttattctc
    13081 ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa aaccagcaaa aaaagacacg
    13141 acctaaagat cgtgtctaat tatgactgta tcagaaaata tgataccaaa cggatctatt
    13201 cattaattaa ttgacatagt ttatctgttg ctcttttatg actggataac tgaccaattc
    13261 atgacgatcc aagtcttctt tattcatatt tacagcagta atttgactgt cttcatacgt
    13321 acgataataa taaatccctt tatcaacgtt acagcaagaa gaataaatcg tatattcgta
    13381 ttttctttct ccagtatcgc atactccttt ttgttgctcg actgagccta gtatatgaaa
    13441 aaattgactg atactttcag attctgaatc acctgatgca gcattcattt tagtaaatgt
    13501 ggcttttaca aatcgagaaa cagaagagag atctccaggt aagcccaatc ctcccatccc
    13561 acgactataa acattcaagc tgatctggtt tgaaaaatga ttttctggtg ttccatttga
    13621 tagcgagcga taattgttca gattaaataa ttgataatca aatggcggat tatttgtcaa
    13681 cacaccgaca ggattatcat ataaatgaag tccatctttc atactttcaa ttactatcga
    13741 tttttcttta tcagctaata accagtgtaa aggtgacaag ggtagttttt cactaaaatc
    13801 gatattgact agattgatag tagccaataa ttctttagct tcgtctatgg ttgagcattg
    13861 ccctagtatc catggaataa actcaaatgg tgcaacgtta tcttttcctt cttgtaattc
    13921 tttataatca gcattgccag gaaaattcag tcctgccata ctcaaccctt tttcatttgt
    13981 agcctcataa tagagtggat agttggatac cccagctgct attcctatca ttgcataatg
    14041 tttatctaaa ttttttacct ttctaaaatc gaaacgataa tttcttggag taatagtgac
    14101 cacttcatta taagacatct cataatcaaa attccttcca aaataatg
//
LOCUS       DACEMO010000060        14066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEMO010000060 DACEMO010000000
VERSION     DACEMO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.061"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(105..1562)
                     /gene="ascB"
                     /locus_tag="IW728_001352"
     CDS             complement(105..1562)
                     /gene="ascB"
                     /locus_tag="IW728_001352"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1024722.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(1559..3454)
                     /locus_tag="IW728_001353"
     CDS             complement(1559..3454)
                     /locus_tag="IW728_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1024723.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(3512..5287)
                     /locus_tag="IW728_001354"
     CDS             complement(3512..5287)
                     /locus_tag="IW728_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1024724.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(5337..6833)
                     /locus_tag="IW728_001355"
     CDS             complement(5337..6833)
                     /locus_tag="IW728_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1024725.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(7027..8649)
                     /locus_tag="IW728_001356"
     CDS             complement(7027..8649)
                     /locus_tag="IW728_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1024726.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(8977..10290)
                     /locus_tag="IW728_001357"
     CDS             complement(8977..10290)
                     /locus_tag="IW728_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1024727.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(10535..10759)
                     /locus_tag="IW728_001358"
     CDS             complement(10535..10759)
                     /locus_tag="IW728_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024728.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(10779..11147)
                     /locus_tag="IW728_001359"
     CDS             complement(10779..11147)
                     /locus_tag="IW728_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1024729.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(11343..12053)
                     /locus_tag="IW728_001360"
     CDS             complement(11343..12053)
                     /locus_tag="IW728_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024730.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(12071..12568)
                     /locus_tag="IW728_001361"
     CDS             complement(12071..12568)
                     /locus_tag="IW728_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1024731.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(12605..13057)
                     /locus_tag="IW728_001362"
     CDS             complement(12605..13057)
                     /locus_tag="IW728_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1024732.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(13192..13797)
                     /locus_tag="IW728_001363"
     CDS             complement(13192..13797)
                     /locus_tag="IW728_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1024733.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT         4137 a         2933 c         2265 g         4731 t
ORIGIN      
        1 atgactatgg tgacttgtga tcgaggagtt ggcaggagaa tactgtttat tcgtaaagaa
       61 ggggctgtga caagactttt gtcacagtcc cttatttcct tttattatag gatttcgcca
      121 tttgattgga tgacttgtct gtaccaatca aatgattttt tctttaaccg tttctttgtg
      181 ccattccctt cgtcatcttg atcaacatag atatatccat aacgttttga catttgtgaa
      241 gtagaagcgc tgacgatatc aatgcagccc cacgacgtat aaccaaataa atctactcca
      301 tctttgatag cttccttcat ctgctcaata tgctgcttca aatagtcaat gcggtaatcg
      361 tcttgaacgg ttccatcttc ttctactttg tctatagctc cgagtccgtt ttcagctatg
      421 aacagtggtt tttgatacct atcatataat tgattcaaag caattctcaa acccttagga
      481 tcaatctgcc agccccattc acttgatgga agatacggat ttttaattcc tccagttatt
      541 aagtttcctt ggaccccttc ccaattttct ggatgagcac tgactgcact agacatgtag
      601 tagctaaaag aaacaaaatc aacagggtag gctgctaaga tttcttcatc acctacttct
      661 atttttaagt tgacaccttt ttcttcaaac attcgttgcg tatacgctgg ataatatcct
      721 cttgcttgaa catcactaaa gaacaaagaa gttctttgct gctcaattgt tttttgaata
      781 tcatccggat cacaagaata cggaacaaga agctgatagc tgatcataca accgacttgt
      841 gaacctttga taatttcatg acaagctttt gtagctaaag cacttgctat aaactgatgg
      901 tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt tttcttcaat tactcctacg
      961 ctaacaaaag ggtgatgttt tacacagttg atctcattga aagtcatcca atattttact
     1021 ttatttttat agcgtttgaa tactgtttca gcgaaacgaa cgtaaaactg aataagcttt
     1081 cgattaggcc atccaccata ttctgtaacc aaatataacg gcatttcata atgggaaaga
     1141 gaaacgactg gctcaatccc atattttaga cattcgtcaa acacttgatc ataaaaaaat
     1201 aatcccgctt cattgggtgt ttcttcatca ccatgaggaa aaattcttgt ccatgcgata
     1261 gacatccgat agcatttaaa acccatttca gcaaataaac ggatgtcttc tttaaagtga
     1321 tgatagaaat catttccgtg tcttttagga taataatact tgctgttggg atcctgcgcc
     1381 gcttttactt gctgatgcgt catcatatgc cattgatctg tccatttttc tttaggtaaa
     1441 tcgctgtcga atgtatacac atcagacaca gataatcctt tttcatctac atcccatgca
     1501 ccttcacatt gattggccgc tgtcgctccg ccccataaaa atccttctgg aaattttttc
     1561 atatcgatcc ccctaaactt gctagttttc ttgtttgatt atttttctta tgactagtaa
     1621 tgatattttt gaatctacta ctatttgtaa caataaccat tgtggtcgga tcatatccct
     1681 ctttgattaa ttcttgcaaa tctacttcca tcaataaatc gcctttacgt actttttttc
     1741 catcagatac tgcactttga aagtatttgc catttaaatt cactgtatca ataccgatat
     1801 ggatcagtat ctctgctcct tcttcactga ctaccccaat tgcatgtttc gttggaaaaa
     1861 ctgttgaaac ttccccgttt acagaactgt agatttttcc gtccgttgga atgatcgcaa
     1921 acccttctcc taaagatcca ctagcaaatg ttggatcgtt tacttcagat aaatcaatta
     1981 tttcacctgc tgcaggagta cttaacacta tatcctttga ggattcttct tcgatttcat
     2041 ccttgtagcc gaaaagaaaa gttagcatac aggaactgat catcgctaag aaataactag
     2101 caactagcca aacaaatcct tcaccaatat aaatcgggaa agacaaaata cttcttgtag
     2161 caactgccaa agcaccggct cctgcggcac cagctactcc accaaatata gctccacata
     2221 tacaagcaat gtaaaatggt cgtttatatt taagagtcac accataaaca gcaggttcag
     2281 tgattccaaa taatgctgtg ataaatgcag ataaagataa actttttact ttcttatttt
     2341 tcgttttcaa aaatacccca agtactgctc ctgcttgcgc agctacggca gtcatacatg
     2401 ctggacctag cgtatcacga ccgtagaaag aaagattgtt ggtcattact ggtacaagtc
     2461 cccaatgtaa cccaaaaatc actaacactt gccaaaatcc gccgataaca gcaccagata
     2521 aaacagggct aaagttataa acagctaaat atccagctgc aatcagctga cttgcataat
     2581 ctgcaattgg acctacaact aataaaataa ttgggaccat cacgatgata caaatgaatg
     2641 gtgtcaataa atttcgaata ctttcgtgca agcttttgcg aatcttaggc tcaatataag
     2701 aaagaaccca tacagctaaa attgctggaa taaccgtact tgtatacctt gctagaatca
     2761 cgggcatccc tagaaatcgc aaggttatgc tatcactata tgcactgaca atcgtaggat
     2821 aaacgagagc tgctgcaata accatagcga tgaaacgatc tgtattgaac ttttttgcag
     2881 cagtatatgc caaaaatata ggcaagaaat agaaaaatgc atcagctgct gcattcaaaa
     2941 tgatataagt tcctgattca gtagttaata aattcaaact cgtacataag gcgagtactc
     3001 ctttgatcaa accacttcct gccaagacgc ctaaagtcgg ggcaaaaaca gcggcgatca
     3061 tgtctataaa agagtttaaa atgccttttt tctcagttcc tctctttcca ctagatgcat
     3121 catcttctag tttcatttgt ttggataata cgtcatacac ttcatttact gcatttccta
     3181 ctacgacttg aaacattcct ccagcttcga ctacagtgat cacaccttct agttgctcta
     3241 ccttctcctt atctgccttc ttattatctt ttagggtaaa gcgtaacctt gttgcacaat
     3301 ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg gacaatatct ttcgctaatt
     3361 gtgaatagtc catttttact tcccccttgt taattaatgc cgtagcaatc cttcttacaa
     3421 gataagtata gttcttagaa aacggtttct ccattcaatt agtacccctt ctaatagcga
     3481 acgaaactgt acaatagtgt gtatggttta cttagccgag caaatcactc actgcttgaa
     3541 agaaaacaag tatatcgttt ctcttatagt caaaggagac aaaaaaaaca cgcttgatct
     3601 tcctttgctg atgtataagt ggtatctcaa gctgatactc tgtgattttt gtttctactt
     3661 ggcttttgat aagacagcac aacaaagtct cggcatttaa cggaagcata actaggtcag
     3721 aactaggaga gattagttgc tgaatggaat ctatcttttt atggattttt atttgatttt
     3781 cttctttgta gtaatgatca agcagttttg ttttcttttt ttcttcaact atggtcagct
     3841 tttcacgcaa aatctgataa atctcccaag attcaaactc atctagaaca ctaggtagat
     3901 aaatcaaatc atcccactca aaaataagct cttgctcagt agtcaaaatt gcatcatatt
     3961 ccgttatttt gtttgattga tttttgaaaa gatacactgg ttctatccgg cattctttga
     4021 tccacatgtt catactttgt tgaatctgtt tttttagtgt attcaaaata ctaattggct
     4081 ggttactaac aatcagtatc tccccttgtt ttcgaaaagg attcaacgct tcggcaagat
     4141 acaaaacaaa ataagcgctt tcagctaaat tgggacgtaa ttgaaaatgt attgggaaga
     4201 aacgccgaat taaataaatt tctaaaggat aacgggagat gatcttttgt acaaaatgat
     4261 tcatggtatt atgtccggca tccatcctcg ttttcatttg tttgatatgg gttagcaaaa
     4321 attctgtttc atggaacaat aacgaaacag gaagatctaa aaaacgaata atcgtttgct
     4381 caaatgctct tagattcttc tctatttctt tgtttttttc atcattaatc atcaaataat
     4441 ttaactctaa tagtcgattt tcaattgctt gtttttcttg attggaaaaa gaatagggta
     4501 gttctgatga taactttttt gtgagggtct ctaccatcgg aataaataat gtatacctgt
     4561 ttgttttttc taaaagttct tctcttcgaa gaatggtcaa aacgagatac cgagaaaatc
     4621 gtatgaagtc ctctcctgaa acaatataat tatatgatat aagaacctct tttaatgtct
     4681 tttggacagt cggcatgata ttggaaaaaa ttgtactgat ttgttgagga aaccttgcgt
     4741 gttggatcac cgtttctgtt ccaacaagag ataagaaaat tctttttgaa ttttcttcag
     4801 caatgatttt tagtcctttt gtcgacgaaa cttccagttc taatttagga ttgcgctcga
     4861 tccaacgcaa aatcgttttt atttgtaatg atacggctgt ttttgaaaga aagaaaatgt
     4921 ctgctatctt ttgcatactg acataccctt tttgaaacaa taagtacgtc acaatagttg
     4981 aagctcggaa atccatgctg gaatagattt tatgtttcgt cacctcttgt atgatttgct
     5041 gtttagtttc agattcaatt gattctaaaa aataacctga acgtttattt gactggataa
     5101 tgattttctt ctcgtagata gcaagcatat ttttgatttc agtttggatc gttttcttgt
     5161 tggtacctaa agcttcagct aattcttcgc ttgttatcca atgattctta tttcttaata
     5221 ctacaagaat ttggagttgt cgtttattta agtccatttg cttacacctc ctctttgtta
     5281 taaacataca ggaaaaacct ctcaaatgac aatagtcact tgcagaggtt taaaaatcat
     5341 ttgaagaaag atctatattc aaggttttta ccattagact ggataatttg tttataatat
     5401 tcaaaactat cttttttcaa tctcctaaag cttccgctcc catcattttg tctatccaca
     5461 taaataaagc catatcgctt cctcatttcc cctgttcctg ccgatacgat atcgattggt
     5521 ccccaccata agtaaccaat gatttggcaa ccatctttta ttgcctcagc aatttcctga
     5581 agatgtattt ttagatactc acaacgtttt ggatcgtgga ttcgattcaa ttcatctggt
     5641 gcttcatcta gtccaatgcc attttcaact atgaataatg gtaattcata acggtctgtc
     5701 aattcattca tgacataacg aaaccctaga ggatcaattg gccaactcca aggttcggga
     5761 gatttttcct tcaaaaaggg attatcgaaa ccatttgctc cgccagtatc tacgcgcatt
     5821 tctgcttctt ttttatagac agcgctacga taataactaa atgcaatata atctgccgtg
     5881 ttagcttgca agcactgttc gtctccatct aacattactg gtgcacagtc ttcttccttc
     5941 catgtcctgt aaatataagc tggataatgc cctcggcaca tgacatcgga gaaaaaccat
     6001 gaattgcgtt taaattgcag tgaaccaaat acattctctg gatcacagtc atatgcataa
     6061 gtcgctaatg cagaaaagct acacataata cccatcttag catttggatc gattttcttt
     6121 aataattgag tagcacgggc gttcgctaga aacatatgat gacaggcttg ataaatcact
     6181 ttttttgtta gtgtagcaat gggttcaagt ttatctctag cagggaatgt aaccaatcca
     6241 gcagctgcat aaggcatacg aaaagcatta ttgatctcat taaaggtcat ccaatatttc
     6301 actttccctt ggtagcgttc aaagacggtc tgcgcataaa attcaaaaaa gtcgattaat
     6361 tttcgatttt gccacccccc atactctcct accaatgcta agggggtttc ataatgacta
     6421 agagtaatta ctggctcgat atcatatcga tttaatgtat tgattagtct gtcataaaat
     6481 acgagaccag cctcatttgg cttctgttcg tctcctcttg gaaatattcg tgcccatgag
     6541 atgcttgttc gaaaagcttt taaacctaac tccttcagta aacgaatatc ctcttcaaat
     6601 cgatgataaa aatcaatcgc ttcatgagaa agataatgga gtgattcgtc taaagcaatc
     6661 tcccaaatct ttttttcttc attccactga atactaggat gctggttcat gatcccgata
     6721 agcgtatctg tcacattagg taatttgccg tcttccaacc atgcaccttc acattgatta
     6781 gcagctactg ctccgcccca taaaaaattt tctggaaata tttcctgatc cataactatc
     6841 tctcctcgct atcaacaatt tcttacttga agtatagctc gattcatcaa aacaagatat
     6901 tctaactatt tcggcagaag agaagaaaaa aattgaatgc tactcttcat ttttaatatt
     6961 tatatataac aaatacataa gacgatcccc gaaagaaaac actcgttaac catctaagca
     7021 aacgatttat tttacttcca acacaaatgc ttcataaggt cgtaaatcaa ctttgccgtc
     7081 tggtaactgt ttcagaggat aatttgacac aataaccctt aaagcttcac gtggaatagt
     7141 gaacgtctgc ttttcactgg acatattaca aataacgagc cattgctggt cttcatatat
     7201 gcgagaatag gcaaagacat gctcaggcgt cttttccact aattcatagt ctccccagac
     7261 gatgatgtca tgttttctac gcaaatcgat caatttcttg tacatataaa aaacagaatc
     7321 ttgatctgct aacgcttctt ctgcattgat ttctttataa tttggattga ctcgaagcca
     7381 tggaattcca gtagtaaacc cagcatgttc acgggcgttc cattgcatag gcgtcctagc
     7441 attgtctctt cctttcgcct tgattgattc caagatttcc tcttttgtaa atccagaaga
     7501 aatcctttcg ttatacatat tgattgtctc aatatctttt gcttccgcga tatcatcgat
     7561 tgggcaattc gtcatgccta actcttctcc ttgatagata taaggggtgc ctttcatcat
     7621 atggagcaag atagcaaaca tcttggcact ttcttttcga tacacatcgt catttcccca
     7681 acgagaaaca attcgcggaa gatcgtgatt attccaaaat aaactattcc atccttcatc
     7741 acctaaagat gtttgccatt tggataatac ttttttcagt tcagagatac tcaagggttt
     7801 caaatcccat ttgtctttgc cttcttgctg atctaatcct atgtgttcga attggaagac
     7861 catggataat tcatttcttt taggattgga ataaagttta gcgatttcgg tagttgctcc
     7921 ccatgtttcg ccaacagtca tcagattttt gtcaccaaaa gttgcttgat tcatttcctg
     7981 aagatactca tgcagtttag gtccattact tgtgatttct ttatccggtt ctttgccaat
     8041 catatcgatc acgtccatac gaaagccccc aataccttta tcgatccaga aattcatcat
     8101 ttgataaatc tcttggcgaa cctgtttatt tttccaattc aaatcaggtt gacgcttgct
     8161 atacaagtgc agaaaatatt gccccgtttt ttcatccaac tgccaagcag aaccactgaa
     8221 tattgagcgc aatctattag gctcttttcc attgaccgga tcacgccaaa tataatagtc
     8281 ccgataagga ttttctttat ttttacgggc ctcaatgaac cacggatgtt catcagaagt
     8341 atgattcacc accagatcca tgatgatacg gatatttctt ttctttgctt cttcaatcag
     8401 ttcttccatt tcttccatcg taccgaattc tgccatgatt gcttgataat tactgatatc
     8461 atacccattg tcatcattcg gagactcgta tactggagaa agccaaattg catcaatacc
     8521 taaatacgcc agataatcca acctggaaat aatcccccgc aaatctccaa taccatcttg
     8581 atttgaatct tgaaaacttc ttggatagat ttgataaaca actgaatggt gccaccattt
     8641 ttgttccatg attttcctcc tataaatgga tgtttctatt ttttgcttaa tcatttattt
     8701 tagtctctct cactaaaaat tgtaacgctt tcaaatagct attgcacctt gttatcggta
     8761 acagaataaa acaaaaaaac agctgaggaa attccctcaa ctgttttttg aaaggattga
     8821 tttatgcagg gaagaatgct gatttcacaa cttctttata aacggtgttc actcagctgt
     8881 cccttggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg tttttttctc
     8941 taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca atatccacat
     9001 gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt ttcctctttt
     9061 agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat ctgaaatact
     9121 ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct ctaagtttcc
     9181 ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca atgctggtgc
     9241 agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta acaagaagaa
     9301 aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac cagctgatga
     9361 ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca tagctgttga
     9421 atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga aacctgcttg
     9481 gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca agaatgtcag
     9541 cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg caactaaaaa
     9601 tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa tcaaacctgt
     9661 gaccagacct aaaatcgttg tagctgccat cccgatgaag atgccacctg gtacacgccg
     9721 aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag aaccaacgtt
     9781 gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac cttcgctcag
     9841 accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca aatctgctgg
     9901 gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa aaatcaaaga
     9961 agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta aacaaacaga
    10021 ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat aacgggctaa
    10081 tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa acactgctcc
    10141 ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat aagccattga
    10201 gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca atttttctat
    10261 ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg aacattataa
    10321 ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca tatactaaaa
    10381 caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt ccatctgatt
    10441 aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat ttctttattg
    10501 tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg attttaccat
    10561 ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga ttatttagtc
    10621 cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt cctgatttga
    10681 gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa taaccttcta
    10741 atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga ctcagctttt
    10801 cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat gccattgtta
    10861 tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc atccgtaaaa
    10921 aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac aaatactttt
    10981 gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt aaattcttct
    11041 ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa tcggcaaatc
    11101 atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat ttgacccttc
    11161 tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg aaagcgcaaa
    11221 caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga ttgctttcct
    11281 ccacaaactt tttttacaaa aaaaaaacgg caatgaatgc cgctcttctt atttcgattc
    11341 ttttataatt ctgcttctga ttcatctttg gttagtgtgt gtctaacact agttacgcca
    11401 aatgtttgat cgattttttc cttcaacgct tcgattgcct gtgcgccacg tgccgcttgt
    11461 tcagcattga tggattcaat gaccagcacc gctgcttgtg tttcttccgt cagcatcgtt
    11521 ctttgggctt cacgccagtt gagacagcga cagatcgctc cttgttcatc atagtagatg
    11581 atttcttccg gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa tgattcgcca
    11641 ccttgggctt ttcctaaatg cagatttcct gcgatctttt cgatatcttc tccgccgcaa
    11701 ggtacgccaa aagacaagga aatactatta tagatatcta caagcggatt gattgggaaa
    11761 aattcccgtt cttgatttgc acgtttcagt aaagcttcaa tagaagaacg tgcacctttt
    11821 ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt ttggctgaat
    11881 gtttcttctg taaggtaatt tcttgcttct tttgctgcct cattcaataa ttgatgatac
    11941 gtttcttcac tttcttttgt tacgtggtta tcgataccat ttacggataa gatttcgatg
    12001 gttgcttcag gaaatacttc ccagaaattg gtatccacta ttactttttt catctgttca
    12061 cacctcttga ttagaataat tgcttgatga atagatagtc tgtttgggga tcatctccca
    12121 tataaaaaac atgttcgctg tatttgataa atcctttttt acgataaaaa gcgagcgcaa
    12181 catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct tttgcttttt
    12241 caatagctgt atctagtaaa aatgtaccga taccttgatg tttaaatttt tttcgtacat
    12301 agattcgctc gatttctaaa aaatgatcag ccatttcctc agattgactc gttcctgtat
    12361 ttagtttcag gtaacccgca ggctcttgat cgatactcac taggaaaaaa tcggtttctt
    12421 tatcttttag ctcttctgta agtttttcta gtgcataagc ttgtgtcaag taatcagcta
    12481 aatgctccgg tgaattatct tttccaaatg tatctgtaaa tgtttcgata ctaatttgtt
    12541 gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc gtatttattc acggcttctt
    12601 cctttcaata gatcctttta ttgccttttt tgacaaaatt ccaatcttcg ctaacattct
    12661 tgcatacttt atttaaaaga tattccaatt gttttgcttc ttcgtcagaa agaccattta
    12721 atgcgactgc attggagtag ttgttttctc gaatgatgaa aggatagatt tctgctcctt
    12781 ttttagtcgg atagagtttc ttgatttttt tattttcttt atcttccaaa cgttcgatca
    12841 tcccattgga ttctaatttc ttgattgctc gggcagtcgt tgtacgatct actttgatca
    12901 tttctgctag tttttcttgg atgattccag gattttcgca aatacgtact aaataaagat
    12961 actgtcctct tgttaaatcg acttctttga attcgatatt gctaatagaa tctagcgctc
    13021 gggcaatcat tccgatgtct cgaaggattt cagccatttc atcccacctt tattttgttt
    13081 gtaagactag ttcacctctt tttgttgcaa atgcaactat tttcaataaa aaaatcaagt
    13141 tcttttttaa taactagtaa agtatattct tttcttcttt tacgaaaaag ctcaatcaca
    13201 atagatctgg tttttgtttt ctaaaataag taactgtttc ttcgttgttc gcttttctga
    13261 tagctttggc aactcttcta tactaaaaaa tcccatgttg gaagtttcat tattctcaat
    13321 aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca agtttataat actgaaaaag
    13381 ctgtggaata tctttttgtt tattcgtatc aaaaactgct cgcaactgag tagtttctac
    13441 tgttaatcct gtttcttcat agacttcttt acgcacattt tcttcaggag acaatcctat
    13501 ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt ttatcttcga ttagtaggac
    13561 cttgccatct tttttgatat aggcacgaac atctactttt ggtgttggat gtccttctcc
    13621 aatcgttgtg agcgctttta tttcttcgaa agtttctttt cctatttcac tgatcagttc
    13681 caatgaaatc gtccggatct cctcaaaacg ctctttatca aatacatctt ttccataata
    13741 tagaccagct tgagaaagtg cgagtagtct tttgtattga gccaacttct tgttcattct
    13801 cttcccttct tttttagcaa atgtatgact atgtaatcaa gtatagaaga ctggtttaat
    13861 tacggcaaat aaatataatg aaatctgcaa taaataaaag aatgttaaaa aactcgagaa
    13921 caaaagacat tcgacttctc tggggctgtg aataaatggc gttcaaaagc ttgcatctgc
    13981 ggtaataagc cccaccaaac gaaaaatatg acgagctatt cttgtctggc tgttcttatt
    14041 aattgaggca ggacagcttt ttcaca
//
LOCUS       DACEMO010000061        13937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEMO010000061 DACEMO010000000
VERSION     DACEMO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.062"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(66..890)
                     /locus_tag="IW728_001364"
     CDS             complement(66..890)
                     /locus_tag="IW728_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1024734.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(863..1543)
                     /locus_tag="IW728_001365"
     CDS             complement(863..1543)
                     /locus_tag="IW728_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024735.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1566..2504)
                     /locus_tag="IW728_001366"
     CDS             complement(1566..2504)
                     /locus_tag="IW728_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1024736.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2749..3600)
                     /locus_tag="IW728_001367"
     CDS             complement(2749..3600)
                     /locus_tag="IW728_001367"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1024737.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3754..6327)
                     /gene="mprF"
                     /locus_tag="IW728_001368"
     CDS             complement(3754..6327)
                     /gene="mprF"
                     /locus_tag="IW728_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1024738.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6468..7274
                     /gene="proC"
                     /locus_tag="IW728_001369"
     CDS             6468..7274
                     /gene="proC"
                     /locus_tag="IW728_001369"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1024739.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7353..8909)
                     /gene="rny"
                     /locus_tag="IW728_001370"
     CDS             complement(7353..8909)
                     /gene="rny"
                     /locus_tag="IW728_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1024740.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9320..10369)
                     /gene="recA"
                     /locus_tag="IW728_001371"
     CDS             complement(9320..10369)
                     /gene="recA"
                     /locus_tag="IW728_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1024741.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10478..11719)
                     /locus_tag="IW728_001372"
     CDS             complement(10478..11719)
                     /locus_tag="IW728_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1024742.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11959..12537)
                     /gene="pgsA"
                     /locus_tag="IW728_001373"
     CDS             complement(11959..12537)
                     /gene="pgsA"
                     /locus_tag="IW728_001373"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1024743.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12746..13630)
                     /locus_tag="IW728_001374"
     CDS             complement(12746..13630)
                     /locus_tag="IW728_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1024744.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         3992 a         3102 c         2432 g         4411 t
ORIGIN      
        1 ttattaaaat aaaaagaagc ctataaaaaa tttttcgttt ttataaggct tcttttcttt
       61 ataatttagt tattttttct tctgaaggca accaatattc gtttgatgat atttacaatc
      121 aggaacaaag caataaagac taatgtgatt gttgctcctg gaggtgtatc tagataaaac
      181 gagctagtta gtcctgacag catccctaga aatcctacac aaacactgat agcaatcacg
      241 acattgaagc ttttgcctaa tcgcataccg attgctgcgg gaagaaccat gattgcggat
      301 atcaaaagtg caccggcaat cgggatcatc acagcaatag ccacaccagt aatcacattg
      361 aacatcatcg acatcaaatg aacgggtagc ccatccacgt gggcggtatc ttcatcaaaa
      421 gtcagaacat acatcggccg tttgaataaa aagaacaata taccgatggc aacgaataag
      481 attcctaaaa agatgacttg ctcccatgta atggtcacga tcgaaccaaa cagataggat
      541 tggatacttg ttgctgaatt ccctccgctc aaattcatca atactaacgc tagcgccaaa
      601 ccaccagcca ttaaaatggc aatggagatt tctgaatagg ttcggtagat cgttcgcaga
      661 tactccaata tcacagctgc taatacaacg actagcatgg tcgtaagtgt aggattaagg
      721 ttcaaaaaga atcctaacgc tacccctgct aaagagacat gagaaagcgt atcagccatc
      781 agcgattgtc ttctgatcac taaaaacaca cctagcatcg gggcgattcc ggcaatgaag
      841 atagcggcaa gaaacgctct tctcatgaat tcatatgaaa gcaacgccat tgagaatcct
      901 cctttctcac cagtcggatc tgtctgtccg catactgttt gatatcttca tgatcatggg
      961 tgatcattag aactgatttc ccatggacat gcgcactatg tcggagaaga cgataaaatt
     1021 catttctgga ttcttcatcc atacctgttg ttggttcatc aagaacaaat aagtcagggt
     1081 cagtagcaaa aatacgcgct aaactgattc gctgcttttg tccgccagac aattcaccta
     1141 ttcgccgatt tcgcatatcc cacatcccta cagcttctaa agcacgctgt acatgttgat
     1201 gatctttttc agagagacgc ttgaaccagc ggcctcttgg atatcttcct gaacggacta
     1261 attcgatcac cgtactagga aaacctgcat tgaaagatgc aacttgttga ggaatataac
     1321 cgatacttaa tttttttcct tcttgatttt tctcagcaat ctttatcatt ccttttgaag
     1381 gtttcagtaa tcctaacgat gctttcacca aggttgactt ggctgctcca ttttctccag
     1441 ttaaaatgac aaattcaccc gcatctacat gataagagac atcttctagt acaggttcat
     1501 catcatagta aaaggtcaaa tcagatattt cgatataacg caccttacaa cctccattct
     1561 aaaacttaat tgatactttc ttttaaagct ttcagatttt tttccatgac agaaatataa
     1621 ttttcgcctg ctttgatttg ttcattagtc aagctttcta aaggattcaa cacttctagc
     1681 ttcactcctg tttcattcga caatgtctca gctaccttcg aagaggcatt ttcttcaaaa
     1741 taaatcactt tgatgttgtt tttcttcaca aattctttta gctcagctaa gcgacttggt
     1801 gtgggttcct gatctggtga aacgcccgaa atcgctactt gttttaagtc gtattgattc
     1861 gctaaataag caaaagcagc atgctgagtg acaaatgtgc ggtttgttgc atcttttagc
     1921 tcatcggagt atttttgatc cagtgcttca agctttttga tataagcatc ggcattcttc
     1981 gtaaaagttt cctttttatc tggatatttt tcgcctaatt cttcagcaat cgttctgact
     2041 tcttttatag ctaataccgg atcgagccaa acatgtggat cgtattcatg tgaatgacca
     2101 tgcccttctt ctgattcttc atgttcgtct gcttcttgcg attctaaacg gtcgattcct
     2161 ttagctgctt cgatcatcaa ggtctgtttg ctatccactg aatcctttaa cgaatccaca
     2221 aaaaattcca tgtctgaact attatacacg aaaacatctg catcggaaat ttctgctctt
     2281 tcttttgcac ttggttcgaa atcatgcggc tccgttccag cagggatcaa aagtttcacg
     2341 tttccttcat cccctacgat ctcttttgta aaatcataca tcggataaaa agtcgtaacg
     2401 atcgtcaaat cctctttttt gtctgcttcc ttgttcgtat ttccacatgc tgcaaacagt
     2461 aaagcaccta tcattaccgt agctgtcaaa agatattttt tcatttatat gctcctttca
     2521 atgaacaaat cggaatttta cgatttatta acgtgaacta atgtatcaaa ataaaaaaac
     2581 acagtcaata gaaatccatc aatttctgat ttaagcatgt atattcttga ttaaatcgta
     2641 aaatggatga gttgctgctc atatctagcc atttcactac aataaaaaac tgaagaatac
     2701 cattttagga ttcctcagtt tttcatcacc tattttactt cttatatttt attgcagggt
     2761 tcgtactaaa taaacttcct cacaaaaacc ttttaaccca ttatatactc tctgcgctct
     2821 tgaataatgt tgacacaagg caaaaacggt tggtccgctt ccactcatca atgcagcatc
     2881 tgctccgtac ttgatcatac gctctttgat ttgatggatg actggatgcc gctttcccgt
     2941 tatatcttct aaactgtttc ccattttttt gatcatctgc tgataatctt gctctcggat
     3001 ggcttcagca agtccgtgga tgtcttgatg ttggatggct tgcgtatcta cttctctaaa
     3061 cactgtacgt gtagagacac tcattcttgg tttgaccaac acaacccagc actgtggcat
     3121 agaaggtaat acttggattt cttccccttt gccaccgaca aatgccgtac ttccgtagac
     3181 acagtaagga acatctgtac cgacttccaa accgatttct gccagttctt ctaacgaaca
     3241 gcccagattc cataattggt tgatccctct tagagcagct gcacagtctg tacttcctcc
     3301 gcctaatcca gctgctactg gaatattctt cttaacaaag atcttgacac cggttttgat
     3361 attgtattta ttttttagca ataaagcagc ttgataaaca ttgttccgtt catctaccgg
     3421 taaaaatgct ttatttgttt ctacaatgat tttgtcttct gttatttctt caaaaaatag
     3481 atgatctgcc aagtctacgc tcgacatcac catttccaac tcatgatacc catcttgccg
     3541 tctatataat acatccagtc ctaaatttat cttggctggc gctttttcaa tcaattccat
     3601 cctctcaccc attcgaaaag tctaagtcgc aagtattcta tcatagggaa aaataaaagt
     3661 atagcttatg tctgcctagt ggttgatttt gtcatcgttt caacagcttt cacagaattt
     3721 tctcttcata atccaaacaa gttctagtga ttcttataat tctgtgagca cttcttctgc
     3781 caaactcgga acatttcctt tttcttttcc tatcagactg agtagctgca tggtagcaaa
     3841 tccgaaatta cttcctttcc tataagcaaa ataacgtcct tcccaatgat cgacatattt
     3901 taatttcttt tcataagcag actggaagga atagaaaaaa ttaccataat tatatacgat
     3961 atgcatgact ttttcttgaa agaaagaaaa atctgtttct ccaacattag ctaatggagc
     4021 taagcctaga ttggcttccg agtagcccat tttctcgtat acaataaaca gattggctaa
     4081 cagatagtca gctaattcct cagattcttt aggtaagacc cgcaacaaat catatcccgc
     4141 ctgcttcttc gtcatcggct gagcggtgat gaagccaacg actttttgat ctttacggac
     4201 aataccgaca ccgctagata atacatatga tttatcaaat cgtcccacag agaagtattt
     4261 ttctcgtgca ccttgaagcc atttttgtga aacttcattt acagcttgat gtaattcttc
     4321 cggcagttga tcgtaccaag taaaatggta tccttctttc atcaaacgtt gatattcaag
     4381 ttgctgactc agccgagaca agtcttctgt tgctagatca acaatccctg cttcacctac
     4441 tttcgtgaag tgaaaaccca attcatgaag catcactacg tagctttctg atatcttata
     4501 aaacgcaagc tgatagccta aacaatctgc ttgtttcata aactgattcg ttgctgcccg
     4561 catcatttta ggattgccaa ctggatctcc taaaacaaag catttgtttc ctttgatctg
     4621 ataaccaaaa acgacttgat cttggtcgtc ttcttggtaa taaaaataac tgtaacctga
     4681 caagcgaagc tgatggcttg tagctgtgcc tccgtatgtt tccaataaac ggtcaaaacg
     4741 gtctttttcg tactcactgc caagcttcgt agtatcttca gctaaatatt gataaagcgc
     4801 cactaaagct agcaatgaga tgcccaaacc aataagccct gagaaccaca catcatctga
     4861 aggaaacaaa ataaaatgat ttcctgttat tcgaccgact aaaaagcgtc cgctgtgata
     4921 gcctgcaatt gcgtaaacga taaacaaaaa gccgaacaag ctagcatcaa agattatagc
     4981 tccccaagaa taaacgaatt ttgttcgata aaattctttt ctggataacc acacagctaa
     5041 cagaatcaag acatatacca atatcaactg ccaagataac gtacgtgtga ttgtatttaa
     5101 aataccaaaa gatagtaaag caatcgtagg cagaaatgcc tttttgactt ttactgaaag
     5161 tgctctagca aggccgagta ataaaaagcc aaccaataga ttcactgtct gatctaaaaa
     5221 attgaatgag aacggcaata atacttgaaa tagccgactg attgcagaca gattagtgat
     5281 tgttgataga agtaccatca tgatccccgc aaaatagaca gcacttacga gcacgatatg
     5341 cgcaaccttt tggaaaaaca gtcgcggaat gttatctaaa aatcgattga ttttgactcc
     5401 tgtatgtgtg atgaacaata gaagtcccgt cataaaaggt agaacataat aaaacaaccg
     5461 ataatataat agccaaacta ctgctacatc ttgccgaaca ggcaattgac tcattcctaa
     5521 gatcatcaaa acgtcaaaag tccccattcc acctgggacc atcgtcagca ttcctatcaa
     5581 tgtagcaatc atgaacatag gatagatcga aagcatcgaa atatctattc tcatcaactt
     5641 gccgatgatc aaaaataccg caagggctcc cgtccattgt ccaaaggatg ccagtagcag
     5701 accaagcaag ccttttggat aaaactcgaa gaacaatgtc tttctcttta gataagcaaa
     5761 aaagaaaatg actggtgcta aaaaactacc gccgaaaagc cagatccaat agcttcgaaa
     5821 aatactttct gactgaataa aaaagacatc aataaatgta agaaacgacc atacagacaa
     5881 accagtcagc ataaatagtg cgacttttga tacagtggct aaaaccattt ttctatttgt
     5941 ctgctttcca taaaaactag cccgcagact tgctcctacc acaccaccaa aaccggcgag
     6001 attgttgatc gtgtttgtta cccacgcagc aagaagaaac tcttttcttt ccatttttgg
     6061 ttttccttgt ttttctaaga ctctgatcgt cacccaatca tacaacaaca tcggagtgac
     6121 acccagcagt ccagccagaa tcatgagcag gatcgtcatg cggctttgtt gtcccatcct
     6181 ttgccaaata tcttgccaac tcatgccatt agcaatattt gcgacttgat ttgccacaaa
     6241 gactaacacg gagccgaaaa aaatcagttt taaaatcaga ctatgttcct ttatccattg
     6301 cagcatcaag tatcgtttat ttttcacatt ttcccattcc taacttgtat acttttgtgt
     6361 cataccccaa tgttatctta tttattttaa tttttccact ttagacctgt tctttgcttg
     6421 cgatgataga aaaagaaaga tatcctaatg ctaaaggaga ggataacatg aaagtaggat
     6481 ttatcggcac agggaatatg gcacaagcta ttattgatgg tttattaaaa aaaggaacag
     6541 tctcgaaaga agaggtgttc gtttccagtg gacattttga aaatgcacaa aaatttgctg
     6601 ctgaaactgg cgtccatgca tgcaaaagta atcaggaagt tgcatcacaa tcagatatcg
     6661 ttcttttagc cgtgaaacca aaaatcattg aaaaagtatt ggaagaacta agagaatata
     6721 gccagaaagt cttattcatt tctattgctt caggaaaatc attaacagat ttagaaacag
     6781 cgcttgaaga tcctcaggcc tccatcgtac gtgtgatgcc gaatgtcaat gtcagcgtag
     6841 gagcaggcgt ttctgcgatt tgcaaaaatg aagctgccac ggaggaacag cttcagcaag
     6901 ctacgatgat ttttgaagca atcggcagcg tctatccttt agcagaatca gatttcagta
     6961 cattcattgg ccttgctggc agctcacccg cattcgttta catgctgatc gatgcgatgg
     7021 gacggactgg tgtactgaat ggtttgtcca aacagacagc cactgagatt gctgcaaaag
     7081 caattatggg ttcttgtcaa aaattccttt caagtgataa aaatccatgg gaacttgtag
     7141 accaagtttc ttctcctggc ggtacaactg ttgcaggtgt agttgcctta gaagaagcag
     7201 gattcatccc agcaatcatc aaaggaatca atacgacgat cgagaaagat cttgagatgt
     7261 tgaataaaaa ataatgatag caatgttttt tcgtgcctta caaaaaaaga agagctgtgg
     7321 cttttgccgc ggctcttctt ttgtattttt tattatttag cataatcagt tgcacgagtt
     7381 tcccgaataa cgattacttt gatgtgtcca ggataatcca gttcttcttc gattcgttta
     7441 cgaatatcac gtactaaacg aacagcgtct aaatccgaga tttcttctgg cttgaccatt
     7501 acccgaactt cgcgtccagc ttgaactgcg taacttgatt ctacaccggc gaagctgttc
     7561 gaaatatttt ccagacttct taaacgacgg atgtagtttt ctaatgattc acttcgcgca
     7621 ccaggtcttg ctgctgagag cgcatctgct gcagctacaa ggacagagat aacagaagta
     7681 gcttccacat ctccatgatg agaagcgatc gcgttgatta ctactgggtt ttctttgtac
     7741 ttggcagcta attccgcacc gatctcaacg tgagatcctt caatctcatg gtcaagtgct
     7801 ttcccaatat catggagcaa cccggcacgt tttgctaatt ggatatcttc gccaagctct
     7861 gctgctaaga taccagctaa ttttgcaacc tcgaccgaat gcttcaagac gttttgtcca
     7921 taactagttc tgaatcgcag acgtccgatg atcttgatta gatctggatg aagtgtatga
     7981 gcaccgactt caaacgctgc ctcttccccg tattcgcgaa tacgttcatc catttcttta
     8041 cgagatttct cgaccatttc ttcgatacgc gctggatgga ttcgtccatc ttgtatcagt
     8101 ttttccaagg tcatccgtgc gatttctctt cggattggat cgaatccgga aagaacaact
     8161 gcttccggtg tatcatcgat gattaaatcg ataccagtca gcgtctcaag cgttcgaata
     8221 ttacgacctt ctcgtccaat gatacgacct ttcatttcat cattcggaag ggtaacgact
     8281 gaaacagtcg tttctgaaac ttgatctgct gcgcagcgtt gaatcgctaa tgataataag
     8341 tttttcgctt tgcgatctgc ttcttctttg gcacgctgtt cagattcttt taccattaga
     8401 gttaactcat gattcagctc ttcttcagtc gatttcatga tgacatctcg tgcgtcctct
     8461 tttgataaac cagcaatttt ttctagttct ttttgttgat cttcaatcat tttttcaacg
     8521 tctttttctc gctcttcaat taattgctgt tttgaactaa gtgcttcttc tttactttca
     8581 agtgagttct cgcgtttttc taacgagtca tctttacgat tcagcgtttg ttcgcgttga
     8641 atcaaacgat tctcttgaga ttttaattct aacttacttt ctctcagctc actttcaact
     8701 tcagaacggt atttctgatt ttcttcctta gcttctaaca atgcttcttt ttttagagtt
     8761 tctgcttctt tttttgcact atcgatgatt ccagcagctg atgattgtgc accagctatt
     8821 tctttctcgt gacgtgactt tgcgaccata aaccctaaac caagaccgac aattaaaccg
     8881 atgatagcga ggagaatgtt gaataccata aaatccacct ccatactatc tttttcattt
     8941 ttatcatcta tacagcttct atgataaatt attctaaact cgtcaatatg catcttcgca
     9001 tatatgtgta caaaaaacat gcacaacttt attctaatgt ttgtaccccg tagtgtcaac
     9061 tttaaataaa caaagaaacg gctttctact tattcattaa acgatggttt ttaaaataaa
     9121 tgacactagt ttcatatagg taaaccttta aattgagaaa cttttctatt aaattattct
     9181 ttgtagatga acagatattt ttattttatg tacccactcg tataattaaa taactcacga
     9241 tttctttatt caataaaaaa acggcaggtc aaggagagta cctactcttc agaccagccg
     9301 tttttgttta tcataatttt tattcttcta ctaaaggcaa ttcttcttgt cctttttcat
     9361 cttctgggac agcgacttct tcaccaatac cataagctgc tctaactaga gcagaaactt
     9421 ctgccatcat ttcaggatga ttagccatat agattttagc gttttcccgt ccttgaccaa
     9481 tccgatcttc tttataagaa taccaagcac cgcttttatc aacaatgtct ttttctactg
     9541 ccatgtctag aagttctcct tcttgtgaga tccctaaacc atacatgaca tctacttcag
     9601 cgattttgaa aggaggtgct actttgtttt ttacgacttt gatctttgta cggttaccaa
     9661 caatatctgt accttgtttc aattgttctg cacgacgtac ttccaaacgg atcgttgcgt
     9721 agaatttcaa tgcgcgtcct ccaggagtga tttctggatt cccaaacatc acgcctactt
     9781 tttcacggat ttggttgatg aaaatagcga ttgtttttgt tttgttgatc gaaccagaga
     9841 gtttacgcaa tgcttgagac atcaaacgtg cttgtaaccc gacatgtgag tcacccattt
     9901 cgccgtcgat ttctgctcgt ggaactaaag cagcaactga atcgactact acgatatcta
     9961 cagccccact tgatactaaa gcatcagcga tctctagacc ttgttctcct gtgtcaggct
    10021 gtgaaagaag tagttcatcg atattcacac ctaatttttg tgcatattgc ggatctaacg
    10081 catgctcagc atcaatgaaa gcggccgttc cgccattttt ttgtacttct gcaatagcgt
    10141 gtagtgcaac tgttgtttta cctgaacttt caggtccata tacttcgatg atacgtccac
    10201 gggggtaacc gccgacacct aaagcgacat ctaacgcaag agagccactt ggaattgtag
    10261 agatttgttg gtcgattttt tcgcccaatt tcataataga gcctttacca tagctctttt
    10321 caattttttt cagtgcagca tctaaggctg ctttacgatc atctgccaat acgataatcc
    10381 tccttatatt tacaaatcag agcaacattt cattatcctt atcatatctt ttttagtgaa
    10441 aaaaagcaag aaaaaaccga acaaatattc gcatttatca ttttcttaaa agctgttttc
    10501 tcaaaagatc aaatccttgc ataaccgcat tatatctgat agcagcacga tctctggcaa
    10561 agtgatattt ttccgaaacc acgccgtctt tagatgaaat agcgatccat actgttcctg
    10621 cagattgacc ttctaacgtg tccggtccag ccactccggt gaaagctaca ctgtaatctg
    10681 ttcttgctat ctcacgtgct tgaatggcca tctgctccac acattctttg ctgactgttc
    10741 catattctgc taatagctcc ggatcaatct ccagaaaacc agcttttgtt tggagcgagt
    10801 aggtaacgaa tccgcctgga aatacttcag atacaccagc aatgtcgcct aatgcggctt
    10861 ggaaggcgcc ggctgtcaaa ctttccgctg ctgttacagt ctgcttgttt tctttcagta
    10921 gttccactac gacttttgct agcgaattat catcgccgta tccataaaaa tattccccta
    10981 ctcgttcttg gattttttct tctaatgcta aaagagcttg attccctgcc tgaacatcat
    11041 ttgttttcac tgtcagtcgt aatgtcactt catttggttt ggcatatggt gcgatcgttg
    11101 gattgatctg tgtctcaatc agatctttta gctcagtgac cagtcgcgat tcaccaatcc
    11161 cataaaagcg caaaacttta gaaattagtt tttcttcaga aggaaaacgt tcctctagta
    11221 aaggacgtac ttgttccaca aacattggtt tcaactcatt aggcggaccg ggaagaagga
    11281 tatacgcatg tgtatctgtt tgataaaaag tccctaaagc aagccctgta cgatttggta
    11341 gaggaacccc gccttcgata atctgggact gttgcagatt gtttttggtc atttttcgat
    11401 gggttgtttc aaaatatgcc aataattttt tgtatccttc cgtattttgt atgagagact
    11461 taccgaggtg agccgcagtt gcttcttttg tcaggtcatc ttctgtcggt cctaatccgc
    11521 cacataagag tatgagttca cttcttgttt cagctagagc aatcaactct tctaaacgct
    11581 gctgattatc tcctacaact gtttgataat aaacttcgat acctagatca gctagctgtt
    11641 ctgacaagaa agtggcattc gtattcacga cttgtcctag taaaagttct gttccgacag
    11701 ctattatttc tgctttcatt acttcccgct cccttatata gtatacgctg tatttttata
    11761 tttttttatt taaaaagagg gcaaggagct gtgacaaaaa tcttgtcaca gctccttgtt
    11821 ccaaataaac agtgttctcc tgccaaaacc tcggtcataa gtcacaatat acataaaaca
    11881 tgaaaagctg ttttttgaaa ttatcccttg tgactcagag ttagacggca ggctcacaac
    11941 ctctccacta aacgatactt acattgatcc tttgaacaca ccgctgtttt tgatgaagta
    12001 atccacacct gaatagatag taaagatcag acaagcataa agcatgattt gatctactgg
    12061 aataccgatc aggctgaatg ggatattatt gataaacagc aagataatag caaccatttg
    12121 tgtagccgtc ttgacttttc ccggccaagc tgcagccatg acttctccat gttcgacaag
    12181 tagtaaacgc aagcctgtga cggctaattc acgacagaca atgatcgcta ctacccaacc
    12241 aggggccttt ccttggtcaa ccaacatgat aaaagcagtc ataactagca ttttatctgc
    12301 caacgggtcc gcaaacttgc caaaattagt gacaaggcct cgcgcacggg caatttttcc
    12361 atctaaccag tctgtgatac tagctaaagc aaaaataact gcacctacta gctgtgtgac
    12421 agccagtgtg gtgtctccta cgttaaattg tccccaatct aaaggtaaac ctacaaccaa
    12481 aatgaaaatc gggatcatac aaattctaag tacagttaat ttatttggta aattcacact
    12541 atcgactcct ttcttcccta ttctacgtga ctatcagcga agtttccacc gatattattt
    12601 acttttccaa atgaatttat tcaaaggagt atttttattg aaattgatct ttttgaatta
    12661 acgaagaagg accgcgacac agatttgtca cgatccttct tttttatacc tacaagtaaa
    12721 cagatgcgta aactaattta tacgatcatt ctgcgtattg aagagtaagg ttcacatttt
    12781 ttggactctg attgttttcc cctggattaa ctggtacgga ttctccattc gctttgatcg
    12841 ttgcattgct tgctgcacca atcgtgatgg tcgcatttgt tgctgtttcc ggtaacacgg
    12901 tactctgtgt ttcatttgct gccagtgtcc cttgataaac ataggcattg tctactagca
    12961 cacctaacca tacacgattg acagcagtaa attcaagggt tacaggcttg gtagcgtctg
    13021 tgatatttac tgcgatggca ctgccagaat cttctcctgt agctattgcc attttctttt
    13081 cttcacttga ttttgttgtt gattctactg tagaagactc tttggtctgc gtgctttgct
    13141 ctgaggaaga ggactcctcg actgatcctt ctactttaag cgaagaagct gtgtctccga
    13201 ttattggatc agcttgacga tcttgccaag tcatatagcc cacgacaacg acaatcgcca
    13261 aagcaaccaa tcctaataaa atgacaggaa gacttgtcca aaagcgactt ggatgcgatt
    13321 cttcctgatg cattgctttt ctagaagtac ttactgtttc aggctgcgga cgttcaggga
    13381 ccgatcctgt caatttgctt ttcccatcaa aaacatcgac taaatggtct ccatcttctc
    13441 ctacagcttc agcgtattgc cgaataaatg cccgcacgta aaatttacca ggcaattggt
    13501 caaaatcatc attgtcgatt gcttccaaat aacgtttttg aatttttgta atctgctgta
    13561 attcatctaa agatattttt ttattcaggc gtgcttgccg taatttttct ccgatggttt
    13621 caaaagccac tcgttcatct tctccagtct gtttgattct ccgaagcatc tgctgttgca
    13681 acgaaagacg ttatgtttgg gtgctgtgac aaaagtcatg tcacagctcc ttttttccga
    13741 ataaacattg ttctcctgcc aactcctcgg tcacaagtca caatatccaa taaatataag
    13801 aagctgtttt ttgaacttgt tccttgtgac tcggagttaa acggcaggct cacgacctct
    13861 ttcttaaacg gtgttcaatc agctgttcct cggtattagc tcaaaaaccg gcaaaatata
    13921 aggagatatt tttccgt
//
LOCUS       DACEMO010000062        13816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEMO010000062 DACEMO010000000
VERSION     DACEMO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.063"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(159..1469)
                     /locus_tag="IW728_001375"
     CDS             complement(159..1469)
                     /locus_tag="IW728_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1024745.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IW728_001376"
     CDS             complement(1481..2899)
                     /locus_tag="IW728_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1024746.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IW728_001377"
     CDS             complement(3139..4707)
                     /locus_tag="IW728_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1024747.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IW728_001378"
     CDS             complement(4711..5121)
                     /locus_tag="IW728_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1024748.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IW728_001379"
     CDS             complement(5118..6041)
                     /locus_tag="IW728_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1024749.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IW728_001380"
     CDS             complement(6038..6916)
                     /locus_tag="IW728_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1024750.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="IW728_001381"
     CDS             complement(6903..8240)
                     /locus_tag="IW728_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1024751.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="IW728_001382"
     CDS             complement(8243..9346)
                     /locus_tag="IW728_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1024752.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="IW728_001383"
     CDS             complement(9420..10928)
                     /locus_tag="IW728_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1024753.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="IW728_001384"
     CDS             complement(10894..11586)
                     /locus_tag="IW728_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1024754.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGSVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="IW728_001385"
     CDS             complement(11608..12582)
                     /locus_tag="IW728_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1024755.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="IW728_001386"
     CDS             12882..13754
                     /locus_tag="IW728_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1024756.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
BASE COUNT         4087 a         2932 c         2278 g         4519 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acactcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaagg
//
LOCUS       DACEMO010000063        13663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEMO010000063 DACEMO010000000
VERSION     DACEMO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.064"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(86..1051)
                     /locus_tag="IW728_001387"
     CDS             complement(86..1051)
                     /locus_tag="IW728_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1024757.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1285..2130
                     /locus_tag="IW728_001388"
     CDS             1285..2130
                     /locus_tag="IW728_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1024758.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2321..3508
                     /locus_tag="IW728_001389"
     CDS             2321..3508
                     /locus_tag="IW728_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1024759.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3514..3870
                     /locus_tag="IW728_001390"
     CDS             3514..3870
                     /locus_tag="IW728_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1024760.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3872..4210
                     /locus_tag="IW728_001391"
     CDS             3872..4210
                     /locus_tag="IW728_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1024761.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            4605..5639
                     /locus_tag="IW728_001392"
     CDS             4605..5639
                     /locus_tag="IW728_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1024762.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5632..6318
                     /locus_tag="IW728_001393"
     CDS             5632..6318
                     /locus_tag="IW728_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024763.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6342..7190
                     /locus_tag="IW728_001394"
     CDS             6342..7190
                     /locus_tag="IW728_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1024764.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7387..8160
                     /gene="sufC"
                     /locus_tag="IW728_001395"
     CDS             7387..8160
                     /gene="sufC"
                     /locus_tag="IW728_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1024765.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8173..9459
                     /gene="sufD"
                     /locus_tag="IW728_001396"
     CDS             8173..9459
                     /gene="sufD"
                     /locus_tag="IW728_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1024766.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9456..10691
                     /locus_tag="IW728_001397"
     CDS             9456..10691
                     /locus_tag="IW728_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1024767.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10678..11148
                     /locus_tag="IW728_001398"
     CDS             10678..11148
                     /locus_tag="IW728_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1024768.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11153..12544
                     /gene="sufB"
                     /locus_tag="IW728_001399"
     CDS             11153..12544
                     /gene="sufB"
                     /locus_tag="IW728_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1024769.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12642..13595)
                     /locus_tag="IW728_001400"
     CDS             complement(12642..13595)
                     /locus_tag="IW728_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1024770.1"
                     /translation="MNVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILPAFGEL
                     RLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTLLPRKI
                     EEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWEDIDYFN
                     KTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMILYGYN
                     TNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLFESGAS
                     IKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4457 a         2529 c         2969 g         3708 t
ORIGIN      
        1 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
       61 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      121 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      181 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      241 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      301 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      361 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      421 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      481 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      541 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      601 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      661 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      721 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      781 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      841 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      901 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
      961 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1021 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1081 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1141 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1201 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1261 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1321 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1381 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1441 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1501 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1561 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1621 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1681 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1741 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1801 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1861 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1921 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     1981 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2041 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2101 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2161 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2221 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2281 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2341 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2401 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2461 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2521 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2581 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2641 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2701 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2761 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2821 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2881 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     2941 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3001 agttggattc aaatcctatc agtttgcacg tattgattca tggctggacc ctttccatga
     3061 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3121 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3181 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3241 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3301 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3361 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3421 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3481 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3541 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3601 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3661 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3721 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3781 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3841 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3901 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     3961 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4021 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4081 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4141 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4201 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4261 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4321 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4381 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4441 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4501 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4561 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4621 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4681 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4741 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4801 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4861 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4921 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     4981 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5041 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5101 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5161 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5221 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5281 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5341 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5401 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5461 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5521 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5581 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5641 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5701 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5761 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5821 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5881 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     5941 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6001 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6061 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6121 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6181 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6241 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6301 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6361 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6421 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6481 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6541 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6601 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6661 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6721 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6781 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6841 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6901 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     6961 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7021 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7081 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7141 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7201 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7261 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7321 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7381 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7441 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7501 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7561 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7621 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7681 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7741 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7801 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7861 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7921 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     7981 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8041 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8101 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8161 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8221 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8281 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8341 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8401 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8461 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8521 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8581 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8641 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8701 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8761 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8821 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8881 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     8941 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9001 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9061 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9121 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9181 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9241 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9301 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9361 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9421 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9481 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9541 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9601 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9661 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9721 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9781 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9841 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9901 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
     9961 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10021 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10081 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10141 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10201 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10261 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10321 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10381 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10441 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10501 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10561 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10621 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10681 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10741 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10801 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10861 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10921 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    10981 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11041 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11101 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11161 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11221 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11281 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11341 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11401 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11461 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11521 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11581 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11641 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11701 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11761 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11821 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11881 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    11941 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12001 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12061 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12121 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12181 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12241 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12301 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12361 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12421 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12481 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag ggtctgtagg
    12541 ttaataacta tttcattgct cgaaacgttg atattaaagc gtttcgagcg tttttttatt
    12601 tgtattttaa tggttggcta cgtttttggc tacgtttgga attattcgcc cataaatttt
    12661 gcaaatcggt caccagtttt cttaacagat tgtggtgtga catgagcata gatattcatg
    12721 gtagttttaa tatctttatg acctaatcgt tcctgtactt cttttatcga cgcaccactt
    12781 tcaaacaata agctacagtg ggtatgccta aatccatgtg gagtaatttg aggctccatc
    12841 ggtgtctttt tgtaaatcca gtctaaccaa tcgtttacta cttgggggta gtacaacttg
    12901 tttgtgtttg tagtgaaaag aaactgtttt ggtgaattcg tgttatagcc atataagatc
    12961 attattttca tttgttcttg ttgccaaata cgcagttgtt taagtgtaaa atcatcaagc
    13021 tgaatcgttc ttgatgaatt tttagtttta ggtacttgta gaactacttg attaaactcg
    13081 tcttgagcga tagttttacc aattgttatt gttttattga aatagtctat atcttcccat
    13141 tgaagcgcca atgcttcgct tttacgcatt cctgtataag ctaataaacg gaaaaaagtg
    13201 tacagtttaa ctgaattatt ttctttagta ttctgtaaaa acagttgtaa ttgatctttc
    13261 gtgtaaaact tcaagggttg ttcttcttca atttttcttg gaagcaatgt tttagacata
    13321 ggattatcct tacagacttc cattgctact ccaaatttaa atatttgagc agttgttttt
    13381 ctcaagaaac catattgttt taatgttttg tgccattcat tcacctgttt ttggcaatag
    13441 ctaacactta ttttatccaa tcgtaattcc ccaaaagcag gtaatatatg gtttttgcaa
    13501 aaacgttgag caacggcaac agaagacggt tttactcttg ttctgtagtt atcaatccat
    13561 aattcataca actcttgaaa agttgtaaca ttgattttaa actcttgttt ttctttaaag
    13621 tctacaagta gtctattaag tgctgtttgc gcctcagctt ttg
//
LOCUS       DACEMO010000064        13530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEMO010000064 DACEMO010000000
VERSION     DACEMO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.065"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            165..683
                     /locus_tag="IW728_001401"
     CDS             165..683
                     /locus_tag="IW728_001401"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1024771.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            811..1494
                     /locus_tag="IW728_001402"
     CDS             811..1494
                     /locus_tag="IW728_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1024772.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1803..1876
                     /locus_tag="IW728_001403"
     tRNA            1803..1876
                     /locus_tag="IW728_001403"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1837..1839,aa:Arg,seq:tct)
     gene            2035..2394
                     /locus_tag="IW728_001404"
     CDS             2035..2394
                     /locus_tag="IW728_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1024773.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2517..4148
                     /locus_tag="IW728_001405"
     CDS             2517..4148
                     /locus_tag="IW728_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1024774.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4210..4851)
                     /locus_tag="IW728_001406"
     CDS             complement(4210..4851)
                     /locus_tag="IW728_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1024775.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            5133..5441
                     /gene="rplU"
                     /locus_tag="IW728_001407"
     CDS             5133..5441
                     /gene="rplU"
                     /locus_tag="IW728_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1024776.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5468..5812
                     /locus_tag="IW728_001408"
     CDS             5468..5812
                     /locus_tag="IW728_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1024777.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5825..6118
                     /gene="rpmA"
                     /locus_tag="IW728_001409"
     CDS             5825..6118
                     /gene="rpmA"
                     /locus_tag="IW728_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1024778.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6211..7359)
                     /locus_tag="IW728_001410"
     CDS             complement(6211..7359)
                     /locus_tag="IW728_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1024779.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7371..7682)
                     /locus_tag="IW728_001411"
     CDS             complement(7371..7682)
                     /locus_tag="IW728_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024780.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(7735..8130)
                     /locus_tag="IW728_001412"
     CDS             complement(7735..8130)
                     /locus_tag="IW728_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1024781.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(8166..8510)
                     /locus_tag="IW728_001413"
     CDS             complement(8166..8510)
                     /locus_tag="IW728_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1024782.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            8811..9032
                     /locus_tag="IW728_001414"
     CDS             8811..9032
                     /locus_tag="IW728_001414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024783.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            9159..9935
                     /locus_tag="IW728_001415"
     CDS             9159..9935
                     /locus_tag="IW728_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1024784.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            9932..10126
                     /locus_tag="IW728_001416"
     CDS             9932..10126
                     /locus_tag="IW728_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1024785.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            10128..10412
                     /locus_tag="IW728_001417"
     CDS             10128..10412
                     /locus_tag="IW728_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024786.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            10433..10621
                     /locus_tag="IW728_001418"
     CDS             10433..10621
                     /locus_tag="IW728_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024787.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            10947..11186
                     /locus_tag="IW728_001419"
     CDS             10947..11186
                     /locus_tag="IW728_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024788.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            11192..11878
                     /locus_tag="IW728_001420"
     CDS             11192..11878
                     /locus_tag="IW728_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024789.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            11881..12711
                     /locus_tag="IW728_001421"
     CDS             11881..12711
                     /locus_tag="IW728_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1024790.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            12728..>13530
                     /locus_tag="IW728_001422"
     CDS             12728..>13530
                     /locus_tag="IW728_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1024791.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKF"
BASE COUNT         4509 a         2194 c         2643 g         4184 t
ORIGIN      
        1 aaactaacgt caattattta taataaaaaa tgagataaag atgaggaaat agaattagaa
       61 acatataatt attaaaggga aacattttta cagctataat ttgaaaaaaa tttatcgtta
      121 ttatatgatt aagaaggatt gaataaagaa agaagttgaa aacgatacct gattacaaaa
      181 aaataacttt tataagttct ataacgttga ttatattttt aattcttatt cttctcgtcg
      241 tcttggttcc ccgaattttt ggtttgactc cttatattat tgaggacaaa gaaatgagtc
      301 catattatgc acaaggaaat ctgatatatg tacgaccgaa aaatacaaat gagattttag
      361 tcggagatat tattacatat tatgaaaatt caggaaaaaa aatctcgacg cgtcgcgtaa
      421 tagccgttga aaacaaccac gaagattttt atacaaaagc tgatacaaag acttatattg
      481 agcctggagt agtaaaaaag agaaatatta tcggtagtcc catctttcag attccatata
      541 ttggactagt cctttcaaaa acggcatttg catggattca ggggttattc tttacgattg
      601 ctttttgcct aacaggaatc acagcatgga atacctttgt agagttgaaa aagaagcgga
      661 gacgaacaca gaaatttttg taagaatgtt aaaaacataa atgtattcca gagatccaac
      721 gactcttctg aacatgaaac acgagaaaac actcatgcag cttgtagatt tagtcagttg
      781 ttcgtataat gtgtgaagag gaggagtagt atgcgcaatg aaatgatgca tcgtcctgta
      841 gtcaaagaag aactattgga ttttatgcgt acccagcaaa aaaaattacc gggtgagcta
      901 ggcaaactgg aagaagaagc tcatgtagca gaagtaccag ttattcccca tgaaacagtt
      961 gtatttttga aatttttact tggacagctt cagcctgaac gaatcttgga gatcggagca
     1021 gctatcggtt tttcatcaag tgtcatggcc acgacaatcg gagaaaacgc tcatgttacg
     1081 acaattgatc gtttcgatgt catgatcgag aaagctaaaa aaaactatga acgtctagga
     1141 ctgacagata aagtgacttt gcttgaaggt caagcagcag atatcttgcc ggagttgtct
     1201 ggtccgtatg actttatttt tatggatagt gcaaaatcaa aatatattga atttttacct
     1261 gaatgcttac gtttgttaag aaaaggtggc gttttgatgg tagatgacat cttccaagga
     1321 ggaacgatct tgctcccaga tgaagagatt ccccgtggca aacgagctat ccatcgtaaa
     1381 ttaaatgaat ttcttcgtgt tgtgatggat catcccgatt tgacatcgac aatcttgcca
     1441 ttaggtgatg gtgtgatctt aatgacaaag gaatcagaaa caatcgagtt gtgatacatg
     1501 attatggcag cctgtagata atcgattgaa tcgagggctc aattcgaact tgatgtaaaa
     1561 gtttctatat ttgtaagtca attaaaggat acaggaaatc taaataaaca ggagtagcat
     1621 gaagtcaatg tagctcctgt tttcaattga taaccttaga acagctgaag gagaatgtct
     1681 cgttttaaaa gattgattta gtagttgggc caaaataatt cattatttgc taattaaata
     1741 actatttcgt attgacagaa gcaatttaat tatctataat tattgatgtt ccgctgattc
     1801 ttgtcatagt agctcagctg gatagagcat ccgccttcta agcggacggt cgggggttcg
     1861 aatccctcct gtgacgctaa aaaaccgtta gaaactgagg tttctaacgg tttttctttg
     1921 ttttttgtgc caatattgtt tgttttcaaa ggcaaataaa ataaaaaaac atccttatcg
     1981 agtacaatta taaggagcaa aaaaataaaa ctcatcagaa aagaggaaga agatatgacg
     2041 atactatcta gaattttagt gactctagtt gcaattgaat ttttttatat catgtatatc
     2101 gaaacggtga gaactgattc tgacacgaca agtagggttt ttaaaatgag taaagaagag
     2161 ttaagcagaa aatctgttca aacattgttt aagaaccaag gagtgtacaa tggactatta
     2221 ggagtgggac tactttatgg agcttatctt tcttccgcat ctaaagaaat cacaagtatg
     2281 ctattaatat ctattttttt tgttgcatta tatggcagtt tgactagcga tcgttggatt
     2341 ttactcaaac aaggtgggct agctctgttg gcattgcttt ctattttctt ataaatatag
     2401 aaaaaataag aatttatcta acaggaaact ttgtgaaaaa tattacttta tctcatgaaa
     2461 tatgatattc taatgaatgg tattttagaa aatttcatga aaaaggagtt agataggtgt
     2521 tcaaaaatta tgtttcctca ctaaaaaaag aattttcagg ttatgatctt tcaagatttc
     2581 agaaagatct tttggctggg atcacagtag ctgctgtagc attgccttta gccttggctt
     2641 ttggggtatc aagcggagct gatgcggccg caggtttgat tacggctatc attgccggta
     2701 tcgttattgg cggtttatca ggcggttttt atcaaatatc aggtccgaca ggtgcgatgg
     2761 cagctatctt gatgtctatt gcggcaacaa agggaatgca aggtgtattg cttgctactt
     2821 ttttagcggg tctattttta cttgcagcag gacttttccg attaggaaca ctgacttctt
     2881 ttatcccagc acccgtaatt acggggttta cttcaggtat tgctatcatt attgcattag
     2941 gtcagatcga taatttattt ggtgttcact cggaaggtac aaacgttatt gaaaaactaa
     3001 gtagttatca aacgatagga tttccgataa acacagcctc tttgatcatg ggagggctag
     3061 tcattttagg gatgtttatc tatccaaaaa aatgggcgaa acgaatgcct agttcgctgc
     3121 tggctatcat tttggcaaca gcagttatgc tattgttcca tttaccagta gcaactgtgg
     3181 gtaagattcc acaaacattg atcagtagta atcgtttgaa tatgggagat tttagctttt
     3241 ctgccttgca acaagtggca gttccagcta ttagcattgc tttattagga atgattgaaa
     3301 gcttgctatg tggtgcttcg gctggccgaa tggcaaataa gcccttagat agcaatcaag
     3361 aattaatggc gcaaggaatt ggaaatctgt tgcttccttt ctttggcggg attcctgcta
     3421 ctgcggccat cgcccgtaca agcgtggcca tcaaatctgg tgcacaaact agattagctg
     3481 gtatgataca tgcagttgtc ttactgctct ccatgcttgt ttttgcgcca gtgatgtcac
     3541 agattccaat gccagcactt gcaggtgttt tgattgtgac ggcttggcgt atgaatgaat
     3601 gggaaacgat caaggagcta tttactaata aatattggtc agcagttcta ttgtttatcc
     3661 ttacgatgat ttgtacagtt atctttgatt taagtattgc tatcgtgatc ggggtgatcg
     3721 gcggctgtgt attttttgtt gtcaaaagcg ctgctatcac gatttcatta gaaacgatcg
     3781 attggcaaag aatggggctg ccagaaacgg aaaagctgaa taactggaca gtagtgtata
     3841 ttagtggtcc actgtttttt atgtcttcag aaaaactcaa aaataaattg gaagaactaa
     3901 atcatcacga tggtgtcatt ttctccatgc gaggcgtacc aagtattgat ttgacggcac
     3961 gtgcagtttt tgatgaattt caagaaaaag cagagcaaag aggacaaacg attatctata
     4021 cttccttgca gccagaagta gaaaaacaac tttctcactt gtgggaacat acattcacgc
     4081 atcaacatca aacagttgct catgcgttga cagctttaca tgcgaaatat actgcaatta
     4141 gccattaaaa caatgaaaaa gaacagacaa gactgtaaac tgaagatttt atggtcttgt
     4201 ctgttctttt taatattgct gtaaaagtga agctagtact tgccagtctt gatggataac
     4261 aggtacaagg gagcgattgt agaaaatagc tgctggatga aaaagtggaa gaattcgata
     4321 gtttttttct gaccattcgt aggcatcctt ttgttctgtt aattgtaaga caggccctgt
     4381 atagagttga ccatgatttt tggtaaccgt atattctttg ccaagtaaac gttgaagacc
     4441 aatgtttcca agtgtcgcaa ttaattttgg ctggaccttc atcagttcgt aatccaatat
     4501 tggtgcatgc gcaaatactt cggaacgagt aggtgtccga tttggataaa cagtttctga
     4561 ctgttgtgtc cgtttattga tccgatgagt cacgcgatac gggcggctcc ttacagcact
     4621 ggtgatatac acctcttctc gtgttaagcc agttgaagaa agtgcctgca tcaattcttt
     4681 tccagcttga ccgctaaaag gaacatgcgt atcaatctct gttttccctg gggcttctcc
     4741 cacgagcatc agtttaggat gaagaggtcc ttgcccagcg ataaatcctt ctacaggacg
     4801 gccgttcatt cttaaagaag cttcttgtat cagttctttt gggtattcca tttatgctcc
     4861 tcctttttct tttattataa gaataaaaga aacacaatgc gactatttta tttggtagaa
     4921 ctgtgaggat agaaaagcag aagtattgct tgaatttttg ttttactttt tgattttaaa
     4981 tttagttgac taacggttgc tgttctggta agctagtact gttgtaattg tggactccac
     5041 agctacaacc gctcagaaca agtaataagc acagcatatg ctgtcacttc ggatggcgag
     5101 tctaagtata tcagaaggag gtgctttata gcatgtacgc aattattaaa actggtggta
     5161 aacaagtaaa agttgaagtg ggacaagcaa tctatgttga aaaacttgac gttgaagcag
     5221 gagaaaaagt tgtttttgac gaagttatct tagtaggcgg agaatctaca aaagtaggcg
     5281 ctccaactgt tgcaggtgca actgttgaag gtactgttga aaaacacggc aaacaaaaga
     5341 aagtcgttac tttcaaatac aaacctaaaa aacattcaca ccgtaaacaa ggtcatcgtc
     5401 aaccatatac aaaagtggtt atcgacgcga tcaacgctta atagcgtttg agaaagaagg
     5461 cctatatatg atcaaaggaa catttaagcg aaatgacgca ggtcagatcg tttcattcac
     5521 tttgaccggg catgctgatg ctggtcctta tggaagtgat atcgtttgtg cgggtgtttc
     5581 tgcgttagcg atcagcactg tcaacgggat tgcctcatta gccggttttg agccgattgt
     5641 tgaaatgaat gaagaagaag gcggttatct ttataccgaa gtcacttcag gaatgacaca
     5701 agaacaaaac aatattgctg agattctatt agaaaatctt ttattaggat tacaatcgat
     5761 agaagctgaa aactcagaat atatccaaat taaaacaatt aatgataaat aggaggtgca
     5821 gaccatgttg ttatctatga atctacaatt atttgcccac aaaaaaggtg gcggttctac
     5881 atctaacggc cgtgactcag aatcaaaacg tctaggtgct aaacgtgctg atggacaaac
     5941 agttacaggt ggatcaattc tttaccgtca acgcggtaca aaaatctatc caggtgtaaa
     6001 cgtgggtatc ggtggagacg acactttatt tgcaaaagtt gatggcgtag tacgtttcga
     6061 acgtaaaggc cgtaacaaaa aacaagtgtc tgtttatcca gtagctcaag aagcttaata
     6121 aataaactgc tctatctctt gtgtagcaag ggatggagct tttttgtaaa ttatgggatt
     6181 ttttttgacc atacttttga ccatactttc ttagaagtta atataatttg ttagtttttg
     6241 tgttgtttct tcttttttgc tgttagtaat gtgtgtatat gtgtccatag tagtttttat
     6301 atttgcgtgg cccattcttt cttggacttc tttgtgatct gcgcctgcct catatagcat
     6361 tgatgcatgt gtatgtctaa aaccatgcag accgatataa ggtaagccgg cacgctcacg
     6421 atatatattg tatctttctg ccactgactg attaattgtg aaattatttt cgtcattggt
     6481 aaacaaataa ttagctttag taaatcctaa ttgaaataag tactttcttt gttcgagctt
     6541 ccaaaatttt agttgcttta tagttttttc atctaaataa attattcgat ttgatttttt
     6601 ggttttagga gtttcagata tataatattt atctgaacga gctacagttt ttttaatggc
     6661 catttcattt gttttaaaat taatattgtc ccaagtgagt gctaatattt cgccgattct
     6721 acatccgctg aatgctaata gtctaaagag cgtatagtct ctgtttttaa aatatggatt
     6781 ctgttcttga gatacttcat ttagaaataa ttccaactgt tcttttgtat ataattttaa
     6841 tttcttttct gttttaattt gtggctttgg aataagtaaa tttaatgttg gattatcaga
     6901 tgttaaacca atatttatag catacttaaa aactttattc atatagttta tgaaaagagg
     6961 gtactgcttt gaagttcctt tttcactcca atcatttact accttttgac aatatgccgt
     7021 attaatttta gaaagcttaa tatttccaaa tttgggatat atatgtttat taaagattat
     7081 tttagtcctt gaatatgaac tttcttttac agtttttttg tagctttcta gccataattc
     7141 tgctgcttct ttgaaagtag tatttgtact tgtgggcatt cctgtttttt gtaattctag
     7201 ttctaaccta gaaagtgctg tttttgcttc tttttgtgtt ctaaatcctc gtttagtagt
     7261 atattttttc tttccagtta gcggatcaat accgagataa gttttaaagt accatgcttt
     7321 ttcgccattt ttctttttat attgttttat cattgccatc aatattaccc ctattcaaat
     7381 tctaattctg ccatgctatc attgaaaaat tcgatcagtc cttgttcctc tataaattca
     7441 agccatattt ccttcccgtt aattaatgga actccttttt ctttacgaat tgtttgatac
     7501 aagttagtca cgacttgttt ccacatttct aatacagctt tatctatttt cttatcatcc
     7561 cagcgttttt tttggggtct atttttgttt ttttcaattt gataattagt agctttgatg
     7621 ataaatgttt cgtatccggt tgtgcttttt tctaaccatt ctttgaattc tgtatatatc
     7681 atttttgttc acctttctat ttttgaacgt atgttctttt ttgcttaaaa aattttagag
     7741 agctgcataa caatagatgc gagcttttaa taattctttc acaagtggtt cgaagcgaac
     7801 gcttaaattg ttatcttcta taaatttcat gtaattgata ttgtgtggtt ctaattcaga
     7861 ctttgcaata tatctatcta ataatttttc aatcatgaag cgatcagcct catattccat
     7921 ttttgaatgg agagagaatg ctaagttata taaaaaataa ttatcatgat gtttagaagc
     7981 gtgtcctaat tcatgtaata aggttctttg tcttctatac ttagataaat tagcttttat
     8041 tactatggta ttcatacaag caacataatg gccatcggca tcaaggtttt cacgctcttc
     8101 taccttaacg ccgagttcat taattatcat ttcaatttga ctgtccaaat gactcaccta
     8161 cttatttagt tatctttttt ctctaaatat gcttcaatca tagctgatag tatttcccta
     8221 tcattatcgg tgagcggttt tccgtcgcta ctcataacag atgctaaagc ttcttccacg
     8281 gttagttttc tttcctctgg tgcaactccc gcgttaggat catctgtgcg tcctagcaaa
     8341 taatctgttg atacatggaa gtagtcagca actttttgca aagtttctat tttaggactt
     8401 tgtgtcttcc atttataaat agagtttttt ccaaatccta ttctattttc taattcagaa
     8461 atagaaatac ctcttttttt acagagctct ttaacccttt cgaaaagcat gtcaaatcaa
     8521 ccttcctaga gcgtacgata ataatttata caaaaagata gaaaggtagt tgacaaatct
     8581 atccaattgt cttatattta actcgtaagc taatttaata agctaacaag caacaagaaa
     8641 tacacctaat aaaataaacg aattcgaggt cggcaaacta agaatgttaa tttttagggt
     8701 ttttctaggc ttatttaact gtatataaat ataagacagt tggatagaaa tgtcaataaa
     8761 aattagctaa ttttattagc ttacatattt aaagaaaagg aggaaacatc atgaaacaaa
     8821 ataaaaaaac tccttccgca aaaggagctt taataacttt aaattttaaa gaccaaaagt
     8881 ttagtgctgt tgttgatgaa attcatccac aagagcatca aaaaaattct tttgcaactg
     8941 acgagaaaaa gactcagaaa cgctctgaga aagttcttcg aataattcaa tttctgttgg
     9001 aaaagattcg tcattttgga agtctatttt gaattcttta ttaacagtct tgaatacggt
     9061 ttgtttgaat aaggcgtcga catccatatt aaattcttcc atactatttt cacctcgctt
     9121 tctattcaaa attatatcaa agaaaggaat gagcaaaaat gaacacaccg caaattttta
     9181 actttgaaca acatgaagta agaacagtaa caatacatga tgaaccattt tttgttggaa
     9241 aagatgtggc taaagttcta gggtaccaaa atggtagtag agatattaac agacacgtcg
     9301 atgtagaaga tcggcaaaac taccaaaacg gtacttttga atcgcctaga gggttaacta
     9361 ttattaacga atcaggtttg tactccttaa ttttaggaag caaacaacca aatgctaaga
     9421 aatttaaacg ttgggtgact agcgaagtgt tgccagcaat tagaaaacat ggaggttatc
     9481 taactccaga aaaagtagaa gaagctttgc ttaatccaga tacaatcatc caattagcaa
     9541 ctaagttgaa agaagaacgt actggaagac taatcgcaga acaaaaaatt gcagaatacg
     9601 aacccaaaat ttcgtatttg gatagtatct tatcttctac agattcagta actattagtc
     9661 aaatcgcggc tgattatggg atgtctccac aacagatgaa caaactactt cataagctag
     9721 gtattcagaa aaaagtaggc aaccaatggt tattatgcaa aaaacacatg agacagggtt
     9781 acacaaaatc tcatacaact gagattccga aatctgatgg cggaacaaaa gttgtgatga
     9841 atacaaaatg gactcaaaaa gggcgtctgt tcatttatga gtcgctgaaa aaagaaggat
     9901 atattccaga aatcgattta ttagaggagg aatgacgatg tcacaagaat ttattttgaa
     9961 agtcagaatt caattagcaa aatacggaaa gtctcagaac tggttagctg atactatcgg
    10021 aatttctaga ccatatatgt cggacattat gaatgggcgt agaaaaccag ataaacaaat
    10081 caagccaatt gaggcagcac tagcagagtt agagaaggag aaataacatg caaataacaa
    10141 ttccagataa tttagtagtt tccgaattaa ctacacagat tacgaatgct gttcttaatt
    10201 cattggacga acgattacac cttatgaaca aatcagtaga gcttcctcca tatccaaaca
    10261 aatcagaggt aaaaaaagtt ttaggcattg gtgatgacaa attaacacat tggataagcc
    10321 taggcttaaa aacacagcag tggagcaagt tagacatcag aattgaacga tcggaactcc
    10381 aaagattttt gaaagaaaac tttgagttct aaaggcaaag gagaatgatt ttatgtccta
    10441 cacattgcaa caagaacatc aaattctagg tctgattaaa cagcgtagaa aacaattaca
    10501 agatgatcgt gcagcgctta gaaaatccga tgagctatca gatagacaag ctgaactaat
    10561 tgcttctgaa cttgaggatt tgagaatgct agaaataaaa aatagggaga ttagattatg
    10621 aagaagacag acacactttt tataggattc attttggggt tattagtgat tgtagcgcac
    10681 caaagtatta tcggaggaag cttgttcgca gcattgatgg ttttaatcaa tctgcttgac
    10741 tcaaaagaaa ggagcaacta tggcacgaga agaagcgcta aaaatcggta aagtgattgc
    10801 tgataattgg tgggcaaata gccgttctat tattttaagc aagcaacata tcgaaaagaa
    10861 aaaagcatgg caacaaataa aaaagtgact ccgccgacca aagcaatgag tcactcaaaa
    10921 aatcataact aaggagattt taacatatgg aaaatgaact ttccactcta gatcaatatt
    10981 tggccgatcc tgattggggc aaatcgaatt tacaactggt tgctaacaag tacgaaaaat
    11041 caattgatga agcaattgtt gcattgactg atgcagcttc cataaccgct gatgacagta
    11101 aatggaccaa gagagacttg ggcattctaa aacgaggcgt taattggctt gaagatcagt
    11161 acagagaaga aacaaaagag aagtgatatg agtgtttaaa ccactaatag attcatactc
    11221 ggcggtacta aagaaattta aaggtaacga cattggtgca acaatcaacg aagaagtaaa
    11281 tatcgaacgg ctgaaaacga tgtatgacgg atatgatggc gatcgagtca ttgaaattcg
    11341 ctttattgat ccacgacggt tcacagtaca gcaaagaaac ttcatctatg cgctcatagg
    11401 cgatattttc atcgatacag gcatgccaac ggacttctgg aaggaattct tctacttccg
    11461 ttttgaaggt gtcacagggc gcgaaataag cctcaaagac gaatcgaata caaccgtgag
    11521 tgatgccaat atcttagcga atatcatcct agatttcatc tttgaacatc atattccttt
    11581 caaagaagga tatgagattt taccagcgaa tcaagagtat tacttctaca aatgcattac
    11641 aaaaagagtc tgctgcatct gtggcaaaac aggagctgat atcgatcact ttgacaaagc
    11701 gctgggaaga cgaaagcgca aagaagttga tcattcagag tacacatttg cagcactctg
    11761 cagaatccat cacacagaga aacacaaaat aggtgtgatc aatttcaaaa ataaatatca
    11821 aatcaaaggg atcaagttaa accaggaaac aattaaaaag ttaaggatag gaggataaat
    11881 ttggctgaga taagttggat caaacttaaa actactatgt ttgacgatga aaaaatacga
    11941 ttaatccaag ctgttcctga gtcggatgcc atcatcgtta tatggattcg attactagtt
    12001 ttagcaggaa agactaacga cgatggtctg atatatatcc agaggaacat gccttatacc
    12061 gaagaaatgc ttgctacatt gtttggcaaa aacgtaaata cggttcgctt agcgctaact
    12121 acattggcaa atttcaacat gattgatcta agcagtgatg gactaattgc catcagtaat
    12181 tgggaaaaac atcaaaatat cgagggtatg gataaagtaa ggctaaaaaa tgctgaaaga
    12241 aaccgtaaat acagggaaag aaagagacag gaacgtctca aattggaaaa tgacgttagc
    12301 gtgacgtcac gtgacggtac agataaagat atagaagaag ataaagatat agataaagaa
    12361 gaaaagaaag gtaagtattc tgacgaacac ttacgccttg ctaaaaagtt gcaaagtaat
    12421 ttaactgaag attttccaaa agaaatgaac aaagtagata tcgaaaaatg ggcagacaca
    12481 atcaggttga tggaagaaag agataaagca tctatagaag cgattgagta tgtgatcaat
    12541 tggctaccta caaatgaatt ttggtttgga aatattagaa gtgctaagaa attgagagaa
    12601 aaatttgaga agctcaaatt cgaaatcaaa gcagacaaga agaatcataa aaagcaaagt
    12661 caaaaactac agtacagcaa tcctagtgaa tatgacgact tgccaattta aaaaggagat
    12721 gcatcacatg gaaagcctag caaatgctat ggagaaacta ataagaagag tattagtgca
    12781 aagcggaaaa tgtccagaat gtagcgaacc tttgtatagt tggcgagcta aaaataagga
    12841 tggttcagaa cgttgtaaac caacatgcat gagttgtggt tataaagcgt tacgtgtgaa
    12901 agaggatata cagaccgaac ggatatataa cgatagctta aaagcacgag cgttgagttt
    12961 ttttcaaaat ggttcggtat taacagataa aactttgttt aaatgcaaaa tggaaaatta
    13021 tcacgtagtg gatcaagaaa caaaaatcgc tttagaaaaa gcaaaaagct atacgaatga
    13081 ggttctgctg aaccatcctg cacatttcat tctatcaggg aaatcaggaa gcggaaaaag
    13141 ccacttatca atggccacag cttgggaaat acttgagcgc tcaaattatg acaagaaaat
    13201 actttttata agctatcaag aattattaga gcaaataaag ttttcttata acaatactga
    13261 actgagaaaa gaaattgaag gatcgcttat agccgatatt aaaacaactg atttggtggt
    13321 ttttgacgat attggagctg aattaggtag tggggtatca aatagtaggc agtttacaaa
    13381 caacacgtta aacacgctct tagaagccag acagaacaag gcaacgatca tcacaacaaa
    13441 cttatctggt cctgaactaa gagaagccta cggtgaaaga attgtttcta ggatatttaa
    13501 gaattcagaa ggttatgcgc tgaaattcca
//
LOCUS       DACEMO010000065        12946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEMO010000065 DACEMO010000000
VERSION     DACEMO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.066"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..149)
                     /locus_tag="IW728_001423"
     tRNA            complement(77..149)
                     /locus_tag="IW728_001423"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IW728_001424"
     CDS             530..1099
                     /locus_tag="IW728_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1024792.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IW728_001425"
     CDS             1086..1946
                     /locus_tag="IW728_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1024793.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IW728_001426"
     CDS             2136..2840
                     /locus_tag="IW728_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1024794.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IW728_001427"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IW728_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1024795.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IW728_001428"
     CDS             4677..5993
                     /locus_tag="IW728_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024796.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IW728_001429"
     CDS             5994..6863
                     /locus_tag="IW728_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024797.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IW728_001430"
     CDS             6918..7727
                     /locus_tag="IW728_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1024798.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IW728_001431"
     CDS             complement(7830..8459)
                     /locus_tag="IW728_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ1024799.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IW728_001432"
     CDS             8629..9921
                     /locus_tag="IW728_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1024800.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IW728_001433"
     CDS             complement(9997..10659)
                     /locus_tag="IW728_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1024801.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IW728_001434"
     CDS             10884..11168
                     /locus_tag="IW728_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1024802.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IW728_001435"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IW728_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1024803.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4315 a         2238 c         2802 g         3591 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacg
//
LOCUS       DACEMO010000066        12847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEMO010000066 DACEMO010000000
VERSION     DACEMO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.067"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..1631)
                     /locus_tag="IW728_001436"
                     /pseudo
     CDS             complement(1..1631)
                     /locus_tag="IW728_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1954..2433)
                     /gene="rlmH"
                     /locus_tag="IW728_001437"
     CDS             complement(1954..2433)
                     /gene="rlmH"
                     /locus_tag="IW728_001437"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1024804.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2861..4150
                     /locus_tag="IW728_001438"
     CDS             2861..4150
                     /locus_tag="IW728_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1024805.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4189..4794)
                     /locus_tag="IW728_001439"
     CDS             complement(4189..4794)
                     /locus_tag="IW728_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1024806.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4999..5463)
                     /locus_tag="IW728_001440"
     CDS             complement(4999..5463)
                     /locus_tag="IW728_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1024807.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5485..5805)
                     /locus_tag="IW728_001441"
     CDS             complement(5485..5805)
                     /locus_tag="IW728_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1024808.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5949..7028)
                     /gene="pepA"
                     /locus_tag="IW728_001442"
     CDS             complement(5949..7028)
                     /gene="pepA"
                     /locus_tag="IW728_001442"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1024809.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7228..7578
                     /locus_tag="IW728_001443"
     CDS             7228..7578
                     /locus_tag="IW728_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1024810.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7801..8748
                     /locus_tag="IW728_001444"
     CDS             7801..8748
                     /locus_tag="IW728_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1024811.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8805..10223)
                     /gene="pepV"
                     /locus_tag="IW728_001445"
     CDS             complement(8805..10223)
                     /gene="pepV"
                     /locus_tag="IW728_001445"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1024812.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10372..11217
                     /locus_tag="IW728_001446"
     CDS             10372..11217
                     /locus_tag="IW728_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1024813.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11221..11661
                     /locus_tag="IW728_001447"
     CDS             11221..11661
                     /locus_tag="IW728_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1024814.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11711..11905
                     /locus_tag="IW728_001448"
     CDS             11711..11905
                     /locus_tag="IW728_001448"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024815.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12041..12727
                     /locus_tag="IW728_001449"
     CDS             12041..12727
                     /locus_tag="IW728_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1024816.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
BASE COUNT         3932 a         2501 c         2380 g         4034 t
ORIGIN      
        1 tcattatttt tatatgttgt aacttcctga taagaaaact aattaaaaga aagaggtaac
       61 tacataaact aattaaaaaa gctaacttca tagttggagg ataaatggaa gaaacttcaa
      121 tgattgtttc tccttgtgtt ccttcaatcg acactagtcc tattatcaat ctctcttttt
      181 tgtccattta cttttgcgac tgttccccaa taatcaaata caggaagcgt tattttttgt
      241 cctttagatt catgtactgt atagactatt ttcgaatcca tcacttgata aggcatagta
      301 tgccattcct tgccaattct tacttcatga ttctgcaaat gttctttgta ttgtaatgct
      361 tccgtgggtg catagtcata aagtcctccg ggaaggagtg tcgtgtaacg aataagattt
      421 tcttgattca ttaccaagtg atctctctga gtgacaactt tatgttcata gtttgctttt
      481 gttgcagcag atacagccaa cagtaataaa acaattgccg ttatattaac tacttttctt
      541 tgcttagctg atgtgctctt catgaaaacg aacgataaaa tcaacgagcc aaacagtact
      601 tgaaagccta agattcgcca aggaaattga atcatttgaa tgggtgtatt ttgtaataaa
      661 gaccatggaa aaaatttggt ggataagagc caagagacta ccgcactaag gtaaatcact
      721 ttttctttgc gattaaatcc tttaaaaaag aagagtggac agatcaaaga aataatacca
      781 atgattccta ccgtaaaggt tgtcaggtca ttattcatcg caccaatcaa actatccgag
      841 acatttaatg cttggtgttg taaggtcgtt ttatctggct cattaatcga ttgaaccatg
      901 atttgtcgga acatcggata ccaaaagtat gctgttaaaa gaatcgtcat tcctactgcc
      961 tttgacaact ccacaatcaa ccggatgctt tctttttttc ttgtttcact tactacaaag
     1021 ccgcctaaga ctagtaagcc aacggctatt acactcataa aagtggaaag aacatgtgta
     1081 taaatgagta agctcatact gactgctaat ggaattgcac atggcttttt ctcataaatc
     1141 agtgcgtaag cataatacat aactatcggt ataaccgtaa gtgcaatcgc ttcagcaata
     1201 gcagaacgat gataaatatc aatcgtacgg tacccataaa atgtatataa acagctaaat
     1261 ataaaagccg catcgattct ttttaaaaat tttagtccat aatgaaacat cagcatgata
     1321 gtacaaacat tcaatagcca aacatataag atataactaa cgattaaatt attacttatc
     1381 caataaaaaa tgactgccgg aaaaaaggtc aagtagggat aaaaataatt cactccgctc
     1441 ccataatgat taaacgtcgt aaaattgatt ggaccagaaa aaattgaaga taaccctttg
     1501 actctagcaa aatggaaggc aatatccccc gatgtagaaa tagtatgaac atttctaaaa
     1561 taattgattg aataaagtcc agcaataatt gctaggagaa tggacaatat tatttttttc
     1621 tttacttgca ttacttaatt atatactcct ttcatggatt gctttatcta gcaaaactta
     1681 aattttaaat aaatcgttat ttatttttac tctcacttaa aaaagcacta aaattataag
     1741 agagggaggt aatggtctga acaccaagaa ttgttccaac atcgaattgc ttaactaatg
     1801 agttcaaagc ttaattatgt ggctcttata tttaccgagc gtggattgcg atctctttag
     1861 cgaaacaaac atctttcata aaaaaaacag ccatatccgc tttcttggat atgactattt
     1921 actactactt tactatcttt cattcttctt gtctcactta tgataaggcg cccccgcatt
     1981 gatccggaag gcacggtaga tttgctcgac aaggatcaga cgcatcaatt gatgtggata
     2041 cgtcattttt ccaaaagaga tctgagcatt gcttcttttc atgacggctt cgctgagtcc
     2101 taaagagccg ccgataacaa aaacaaggtg gctttttcct tggataccta attggtcgat
     2161 ttgtttagca aaagattcac ttgtaggatt tttcccttca attgctaagg catagacgta
     2221 ttcttgttct ttgattttcg ctaagatccg ttccccttct ttttctttta cttgtctcat
     2281 ttgcgcttcg cttaaattct ccggggcttt ttcgtcagga acttcgatca attcgatttt
     2341 tgcataggca ttcagccgtt tgacatactc attgatacct tggatcaggt atttttcttt
     2401 taattttcct actgagataa ttttgatatt catgttttct ctcctttggt cttcatgctc
     2461 actttgttta ttataatcaa ttttttcgtc tatatgttta ttttggctat ttttgttaaa
     2521 aagatttccc caacagttat gcacaacatc cacaggttta tccacagaaa acagtgttat
     2581 tttaccttat ttcatcacat tcattaggca aacatacgtt ttataagaat ttatccactt
     2641 tatacacagg ctgtggatat gttttatccc ttatttttcc aatcctatcc gcgtagtttt
     2701 acacagtatc tgtggaaaaa gaaaatcaaa tattttcatc tgaaaacaat gatttttcct
     2761 aaagtttttc ataaaaagtt cacagtctca aagtagacta aagtcaacaa ataagactat
     2821 actaacagta gataaatgaa tgtaattgga ggaggaacac atggatagaa aaaatgtgac
     2881 accaaagatg aaaaaaagta aaaataatag cctctggcgt aagttaggtc tggggcttgt
     2941 tggagggatc gttggaggac tactgacagc tggcattttt tatgcagcga tgggcactgg
     3001 caacaatagt tctactgcta caagcggcaa tcagaattct gccggtgaaa cagttgtgga
     3061 aaatgtcaaa gtcaatgtag attctgatat caccaaagcg gtagataaag ttcaagacgc
     3121 tgtcgtttct gttatcaact tacaaagtca aaaccaatca agcggttttg gcggactatt
     3181 cgggcagcaa gaagaaagta gcagctcaga tgatagtaat ttggaagctt atagtgaagg
     3241 aagcggcgtg atctacaaaa aagacgggaa tactgcttat gtagtcacga ataatcacgt
     3301 agtagatggc cagcaaggtc ttgaagtctt gatgaaagac ggtacgaagg taaaagctga
     3361 attagttgga acagatgctt attcagattt agcggtgttg aagatcaatt cagacaaagt
     3421 cgaaacagtt gcctcttttg gtgactcaag tgctctgaag gttggtgagc ctgcgattgc
     3481 gattggttcc ccactaggat ctgaatatgc aaattctgtc acttcaggaa tcatttcttc
     3541 cttgaaccga caagtgacaa gtacgaatga atcgaacgaa acagtcaata tcaatgcgat
     3601 ccaaacagat gcagcaatca accctggaaa ttctggtggt cctttagtta atatcgaagg
     3661 acaagttatc ggtatcaact caagtaagat cgcaagtact tctgaatcta cttctaatgt
     3721 aagcgtagaa ggaatgggat ttgctattcc aagtaacgat gtagtgaata tcatcaatca
     3781 actggaaaaa gacggaaaag tgacacgtcc agctctaggt atcacaatgg tcgacttatc
     3841 tgccgtttct actcagcaac aagaacaaat cttgaagatt ccttcatctg taactaacgg
     3901 tgtcatcgtg acaaatgtcc aaacagctac accggctgaa aaagctggat tgaaacaata
     3961 cgacgtcatt actaagatcg atgacaaaga agtttcttct ggtgtagaac ttcaatctgc
     4021 tttgtataag aaaaaagtgg gagattctgt caaaatcact tactatgaag gctcgaaaga
     4081 aaaaacaact actgttgaat tgacgatcga tcaatcggca ttaaaacaaa atcaaaatag
     4141 tgaaaattaa taaaaaagag gttgtgacaa agacttgtca caacctcttt attttggaaa
     4201 tgcttctccg actacggcat cctcaggcaa tactaagatt cctttttttg ctggagcatt
     4261 tggcaaatgc aattcacgag cggaacagat cattccatag ctatctaccc ctcgcaaagt
     4321 tcctggccag atcatcattc catctggcat catagctcct ggttttgcaa cgacaacttt
     4381 ttgtccagct tcgatattgg gtgcaccgca aacgatttgc aatactgtac cattgtctac
     4441 ttctgtctca gtaatcgata aatggtcaga atcaggatgt gggacgcatg tttttacgta
     4501 cccgacaacg atcttcggtt catgatcagc ttcaagctgt tcttcgaatc ctgcttcttt
     4561 gatcaatcga ttcaatttcg ctaattcctc atctgttaag cttacttggc cgttcccttc
     4621 gattgttaga tgatcggaga tatggaaaaa gttccaagca acaacttgac cttgttcatc
     4681 tttgatccga gcaacattcc atttgcgttc aacagtgttt tctgttcctt tatcgtcggc
     4741 tataataacc atcaaggtgt ctccgacgtg tgctttgtta taagcaaaaa tcatgttctt
     4801 tcctccatta tcctttgctg agatttatct taacaaaaaa ttgcattcac tgcatgaaat
     4861 ttttttcatg ctttatttcc gaagaagcct cttctggaac accatttatt cgtaacaagg
     4921 gactgtgaca agacttttgt cacagtccct ttactgtttt agttaattga ttaaaaaggt
     4981 cgtacaagtt ccacttgctt aattacttgg ctgttcttct tcgtcagaaa cgatcaagac
     5041 atcacaaggt gaatgacgta tcacgtagct ggctacactt cctgtcataa agcgttcgac
     5101 tgcatttaga cctgactgcc caaccatgat tagatcgatc tcatattttt ttggcaattt
     5161 gactgccatg gattcacgaa cagaaccata tgcaataatt ccttctatat ttgtgtaatc
     5221 aacagatttc ccatattctt tacattcttc gatcatttcc ttggctgttt cagtttgctg
     5281 atcgactaga ttttgatcaa taggtgcata gcccataaag ttataaaatt gctgatcgat
     5341 tacgtttgct acatatactt tcccattatt acggcgagcc acttctaccg cttttttgaa
     5401 agcagcaagt gcttgttcac taccgtcgat acctactaaa atattcttat attcttgatt
     5461 catcttgacc cctcctaaca gatgtcagcc ttttgttaca ctttctagga acgattcgat
     5521 ttcctgtttt gttttacgat ctttattcac tagacgacct aattcttttc cgtcttggat
     5581 cacgacaaaa cttgggatac caaaaatgtt ccattcagcg gctacatcta tgtacttgtc
     5641 acggtctact tcaataaatt gccaatcaga aaagtcattt tcaatttcag gcatttgcgg
     5701 tttgataaaa cggcagtctc cgcaccagcc ggcagtaaag aaaaatacac tctttccagt
     5761 ttctacgtag cctgctaatt cttctaaact atttgggata atcattcttt gtcctctact
     5821 ttctgtttat gtatttttca attaaattat acacctttct ttcgaactaa caaacattga
     5881 tcctcttttt ataaaaaaaa tctggcaaaa agcaatcttt tgccagattt tatagtttca
     5941 ctatcgcatt attttccgta tacgatcgta tttactgttg atttatccaa acctttcagt
     6001 gtttgaatca acatttctcg tgcagcttca aaatcgcgaa tactgaacat cgtttgatgc
     6061 gtatggatat aacgtcccac tacgccaatc actgtacttg ggattccttc gttttgtgta
     6121 tgagcagcac ctgcatctgt accgcctttt gaaacaaagt attgataagg gatattgttt
     6181 gtttcagcga tatccagcaa gtactcgcgc aaacgaggaa gcatgatcaa acctggatct
     6241 tggatacgca tcaatgtacc ttctcctaaa tgtccgaatg tgccattttt cgtcactgta
     6301 tcatctgctg cagagcagtc tactgcaaag aacagatctg gcttgaattt attgacagaa
     6361 gctttagaac cacgcaagcc tacttcttcc tggacattcg cgcctgcaat caatgtatga
     6421 cctaatgttt cattttgcaa cgcttctaaa gcttctaata ccattgtaca gccatagcgg
     6481 ttgtcccatg atttactgat aatattcttt ccgttggctg ttttgatcgt ttcagcgtaa
     6541 ggaacgattg tgtctcctgg aaggacaccg taactcattg cctcttctct tgattcaaac
     6601 ccagcatcga acaaaatatc ggttacttct actgattttt gtccgcttgt tcctcgcaat
     6661 aaatgtgggg gaacggaaga agaaatacat ggataattct taccagtaga ggttttaagt
     6721 gtaaaacgct gagcagaaac aacgtaagga ttccagccgc ctagaggtac gactttaaaa
     6781 agcccgtttt cttggatttg tgtcagcatg aatccgactt catccatatg tgctgcgacc
     6841 attacacggg gtgcatccgc ttcttcatga tggcgcagac caaaaatgcc gcccaaacca
     6901 tctaactgta actcatctac cagcggttcc atatgacttt ccatataagc gcgaacatct
     6961 tgttcaaaac cgcttgttcc ttgtaattct gttaactctt tgattcgttg aaatgttttt
     7021 tcttccatat gctattcgtc taaatttcag actaaactcc tctcagactt atattgatct
     7081 gcggataaaa tatgattttt tctgcagacc gatcgtattt attatagctt attttttctg
     7141 gtagcggtta tttatttcaa agaaaatatg agagtgtggt aaaataagca caaggagtaa
     7201 caagtgagaa gaaggaggta tgtccacatg tatgaagaaa gcgaactaag ctattttaaa
     7261 ggcgggttag cagttggaat cggtctagga ttattgagcg gggtagcttc aacgatttgg
     7321 tatcatcgca aaaaagtatt gtcagcagac caagtacttg aaaatatcaa gtcatctttt
     7381 ctaaaagaag gtgcaatcga aggttcttgg atctcttttg aaaaggaacc tattaggaaa
     7441 ttcgccgttc ggtcgagtgc ttatcgggga ggtctttctc gaatagaaga tggcgaaact
     7501 gtttattatg aatttttagc agatgctcat acaggaaccg tattagaaat ttcaagaaaa
     7561 aaaggtgcaa cagattaata cgtcaaaaaa gtgagagaac gaatacgcac ccactattca
     7621 aaagagaggt atcatgtcct tcttttgtag tgggtggttt tttagataga gctttccaaa
     7681 catatattcc agtattttct ttttaaataa atgatattta gcgggaaaaa ctagcatttc
     7741 ctttgattat gaggtatttt taatttagct tttgttttct atagttaagg agagtgaaaa
     7801 atggaacaac aagaaaaaat tgctgttctg attccttgtt acaatgagga agcaacaatt
     7861 tcaacagtca ttgcagactt taaacgagaa ttaccagaag cagatattta tgtctatgat
     7921 aataattcga ccgatcgaac atacgaattg gccgttcaag gtggagcaat cgtaaaaaaa
     7981 gaacctcgtc aaggaaaagg aaatgtgatc cgccaaatgt tctttgatat cgatgccgac
     8041 tattatttaa tggtagatgg cgatgatact tatcctgcag aagctgtaca tggtttgctc
     8101 gagaaattac gttctggtga agcagatatg gtcattggtg atcgcctatc aaatggaacc
     8161 tactttgatg agaataagag acctttccac gattttggaa ataaccttgt tagaaataca
     8221 attactagaa tgtataaaac aaaaattctt gatgtcatga ccggctatcg aggattcaat
     8281 cgtatctttg tcaaaagttt tcctattatg agttctggtt ttcaaatcga aacggaattg
     8341 acgatccatg ctttagataa aaaattcaag ttcgttgaaa tcccaatcga ttatcgtgat
     8401 cgtcctgaag gcagcgaatc aaaattgaat acattctctg acggattcaa agtgatcatg
     8461 atgattatta aaatgtttaa agattataag cctttgcttt tctttgggat ttggaccatc
     8521 ttcttctttt tatttggatt ggtcacgggg attcctgtga ttcgcgaata tatgctgaca
     8581 agctttatta cacgcatccc ttctgcgatt ttaagtactg gattgatgac tttagcactt
     8641 ctttcattag taacagggtt gattttggat acagtcgtca cgaacgctaa gaaagaatat
     8701 gaattgaatc tatatcatgt ttatcatgaa taccacagta gaaagtaaaa aaaggatcaa
     8761 gactgcaaaa ataatttgca attcttgatc ctctttttaa ttaattattt cgccaaacga
     8821 tagatggcat ctgcatagat tgctgttgct ctgaacaagt catcaagact cataaattcg
     8881 tttgcttgat gcattgtatc agtatatcct gggaacatcg ctccataagc aacgccacgt
     8941 ttcaacaaac gtccgtaagt tccgccaccg atgatttgtt cgtagccttt ttctccagtg
     9001 tggtcttcat agacttgcaa taatgtagcc accaatggat catccattgg tacgtaatgt
     9061 ggttccatta cgcgagcacc tcgagtaact gttgcgcctt cttgccccac tgtttgttcg
     9121 atgtcacttt gcaatccgtc tactgttaca ccttttggaa aacggaaatt catattgatg
     9181 aagttcgctt cctctgtacc attttcttca aaagcaaata gtccagcatt cattgtcaat
     9241 ttgcccattt tttcatcttc ataagcgact cctagttttt ctccataaaa atcttcatgg
     9301 acataagcag ctgctacatg gataaattgt ttagcagatc ctaggaattc atagttatct
     9361 aagaacaatg ccaagaaaga acctgcattg ataccagatt gtgggcttgc accatgtgcg
     9421 cctttaccaa ctaattcgat ttttacatac gtattgtccg cttcaatcgt tccgcttact
     9481 ggatttgctt cgatgaattg gtaaaatgct tcttccatcg cgatagctgc atctgcactt
     9541 ggtacttgaa gtgctgcagt tgctgttcct ggcaccatat tttcgcgaag tccggaaaca
     9601 aaagatttca agacataatc tcctgcattg tcgcctttga agcgtaaagc taaagaaaca
     9661 tttccttttt caccattgat gattgggaat tcagcatctg gtgagaaacc aaaatctggt
     9721 tcttcttcat gttcgaaata ataagccata tcgccccagc cgctttcttc atcgctgccg
     9781 actacgaagc gaactttttt tgatagtttc aagtcaagtt ctttaatgat cttcatcgca
     9841 tagtaagctg ccatacttgg acctttgtca tcgcttgatc cgcgtgcaaa gatctttcca
     9901 tccttgatta ctggttcata aggatctgtg tcccagccat ctcctgcagg aacgacatcc
     9961 atgtgtccaa atatccctag cgtttcgtct ccttcgccaa gatcaatatg ccctgcatag
    10021 ttatcgacat tttttacttt aaatccatca cgttcgccat aagctaacat atgtttcaat
    10081 gcgtcgcgag gtccaggtcc aaatggtgca tctggtgtta cttttgagtc atcgcgctcg
    10141 ctgttgacac gtaaaagatt tttcaagtct tcaagcaagt cttctttacg tgcttctact
    10201 tctttttgcc aatcaattgt cataaagtta atcctccact tatttaagtc ttcgtgttca
    10261 tgataccaca aattacagaa aaaaatagtt caaaaaatct gacaattcct tttcttatct
    10321 gtttttctct aaaaaaaata tacgctataa tgagtaaaag gaggttttta gatgctgata
    10381 aacgaacaac tagtgaaaag agtcatcaca cctcgtccaa aaatttcaca taaaggaaca
    10441 tttggacgta tcgttttgat tggcggcaat gaacaatatg gcggagcaat cattatgagt
    10501 gctttagctg ccgtacacag cggagcgggt ctcacgactg tagtaactga tgaaaaaaat
    10561 cggagtgctc ttcacagtca tctgccagaa gcaatgattg tagtttggac ggatactgac
    10621 cagtttcttt ccgtaataaa gcaagcagat gtactcttga ttggtcccgg attgggtact
    10681 tcttctaaga gtttggaacg tttcaaacaa gtactagaaa accaatcaaa agaacaatgg
    10741 ctggtgattg atggctctgc tttaagttta cttgctgcta atcatctttt gctaccctat
    10801 cccgaacaaa ctgtacttac acctcatcaa atggagtggc aacggttgag ttctctaccg
    10861 atacaagaac aacgggataa agaaaacaaa aagaagcaac aggaaatcgg tgctgtgctc
    10921 gttttgaaaa gtcatgagac aaaaatttat ggtcaccagc acttctacac caatccttta
    10981 ggttctgctg ctatggcaac gggcggcatg ggtgacactt tagctgggat gatcacagga
    11041 tttttagccc agtttcctca aaatgaccaa acgattgcag cagctattta tcttcatagt
    11101 ttcattggag accagctggc aaaagaacaa tatattgttt tgccgacaca aatcagcgaa
    11161 gcacttccga aatggatgaa ttatttttgt aaaaaatcaa tggaaaacga ggcatgagtc
    11221 atggagaaat ttacaggtta tgtgcgagaa cctttttatt atgaaacaga tcaaatgggg
    11281 attattcatc acagcaatta tatccgctgg tttgaagagg ctcgggtagc cttattggta
    11341 catctagggt ttccctataa aaaaatcgaa gaatctggga tcatcatccc cgttctggaa
    11401 gtaaattgca gctataaaga aatgatccat tacggagaaa aagtgagcat ccatccaact
    11461 atccaaaagt atacggggac acggttggat tttagctacg aaattgtggg aactgaagac
    11521 aaaaaattaa aaactactgg cagtagtaaa cactgttttt tactttctga ttcgcaacga
    11581 ttagtgaagc tgagcaaagt caatccggag ttagatcagt tattccaagc gtacgcttct
    11641 ttggatgatg taaattctta ataaagaccg tctaagcatt tttcttgtat tgtgtcgagt
    11701 agttcgtata atgaaaagaa aaaagcaggt gatccccatg tatgtcgtta aagtaatgca
    11761 cggttacatc gataaaactg gctgtcgcac tcgggaaaaa aacttagaca atctgctcat
    11821 tttcaaagac aaaaaagaat ccgaagcttt tgctaaaaga atcggcggtc gtgtcaaacc
    11881 gatccaagag gtacgaccag attgattcaa tatttttctt ttcgaggctc taataggcat
    11941 gcctatcagg gctttttttg cagactgtgt ttttatgccc acacgatttg taagcgattt
    12001 atgttataat agattatatc tgcatagaaa ggaaggttaa atggttatca ataatttgca
    12061 acagcaagct tatgaatcta tacgtaaaca aatcatttat tccgagttag cacctggaag
    12121 taaaatctcc gataaagtgt tagaagaaca actagctatc gggcgtacac cgattcgaga
    12181 agcactgatc caactgcgca atcaagagct tgtaaaaacc attccacagt ccggcactta
    12241 tgtttcaaaa atcgatatcc gatctgcttc attagcccgt tatacgcggg aaaagttgga
    12301 aaaaccgatc ctgcaagaat gcagtgcgaa aatgaccaag caagatcaag ctaaactaga
    12361 aaaaatacta gaacaaaccg atcaggcagt aatcgctcaa aataaaaaag ctttttttga
    12421 tttggatact gattttcacc gaacttgtta tgaaattgct ggaaaacgag aaatttggga
    12481 ttggctagaa agttatagta cgcacttgaa ccgtttcaga tggttacgtt tgacgatttc
    12541 agaattagac tggggtcgag tattagatga acatcagaca atgcttcaat caatgattga
    12601 tcataacttt gatgaagtcg gatttctttg cacgatgcat ttacatatga ttatcgagga
    12661 gcaagaatac gtgattcata attaccccga ttattttgaa gatattcagg acagacccat
    12721 caaataagag gttttggaat cagcgttttg acctgagtat tagagaaaga aacggcaaaa
    12781 tagttcctca tattttgccg ttttttttga gctaataccg agggtcagct gattgcacac
    12841 cgtttat
//
LOCUS       DACEMO010000067        12727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEMO010000067 DACEMO010000000
VERSION     DACEMO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.068"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..802)
                     /locus_tag="IW728_001450"
     CDS             complement(83..802)
                     /locus_tag="IW728_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1024817.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(851..2497)
                     /locus_tag="IW728_001451"
     CDS             complement(851..2497)
                     /locus_tag="IW728_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1024818.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2676..3266
                     /locus_tag="IW728_001452"
     CDS             2676..3266
                     /locus_tag="IW728_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1024819.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3358..4863
                     /locus_tag="IW728_001453"
     CDS             3358..4863
                     /locus_tag="IW728_001453"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1024820.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(4947..6299)
                     /gene="celB"
                     /locus_tag="IW728_001454"
     CDS             complement(4947..6299)
                     /gene="celB"
                     /locus_tag="IW728_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1024821.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6299..6817)
                     /locus_tag="IW728_001455"
     CDS             complement(6299..6817)
                     /locus_tag="IW728_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024822.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(6833..7159)
                     /locus_tag="IW728_001456"
     CDS             complement(6833..7159)
                     /locus_tag="IW728_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1024823.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7172..9151)
                     /locus_tag="IW728_001457"
     CDS             complement(7172..9151)
                     /locus_tag="IW728_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1024824.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     EVVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9172..9486)
                     /locus_tag="IW728_001458"
     CDS             complement(9172..9486)
                     /locus_tag="IW728_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1024825.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9654..9947
                     /locus_tag="IW728_001459"
     CDS             9654..9947
                     /locus_tag="IW728_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1024826.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10025..11137)
                     /locus_tag="IW728_001460"
     CDS             complement(10025..11137)
                     /locus_tag="IW728_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1024827.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11524..11703
                     /locus_tag="IW728_001461"
     CDS             11524..11703
                     /locus_tag="IW728_001461"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024828.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            11897..12067
                     /locus_tag="IW728_001462"
     CDS             11897..12067
                     /locus_tag="IW728_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1024829.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12491..>12727)
                     /locus_tag="IW728_001463"
     CDS             complement(12491..>12727)
                     /locus_tag="IW728_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1024830.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4019 a         2556 c         2152 g         4000 t
ORIGIN      
        1 gccatccgaa agtcagacgg acaagtctgc ttttcagatg gcgctacaac tactctgctt
       61 attcttttag ctatccattc aattattttt ctctcacttg ttctagctct tctttggtca
      121 tgggtcgata ttgcccttta ttcaaatctt taggcagcca aaattttccc atacggattc
      181 tttttaaata taagacttct tttcctactg ctttgaccat tcgcttaact tggtgaaact
      241 ttccttcatg caaaatcaga cggataattg aagtttctgt ctcttgatct acttgatcaa
      301 tttggagttt tgccggcaat gtctgctcat cgccatcaat aatcagtcct tctgcaaata
      361 aacggatatc ttcttctgtc attataccgc taacctcagc tagatattct ttctccacat
      421 gttttttagg tgatagtaac tgatgggcaa acaccccgtc attcgttaaa atcaagaggc
      481 cctctgtgtc tttatctaat cggccgactg gaaaaagatc ttcccgataa tcctggtctt
      541 ccaataaatc gattacggtt tgatcatgat tatcctgagt agcagaaatg actcctgcag
      601 gtttatgcaa aatgtagtaa aaatcttttt gatacgtaat gatttctcct aaataagtaa
      661 ccgcatcttc gaattcattt acttgcgctt tatcgctttt caccacttgt ccattgactt
      721 cgatcgttcc ttttttgatt aatcctttga cttctttgcg gctgccaagt cccatatctg
      781 ctaaaaactt atctaaacgc atgcgtttcc cccatttcaa aagagcatca atctagctct
      841 tctgattcac ttattttatt tttaatttat gtcgtaaacg cgctattcct ggtcctagta
      901 gtctttctgc caagcggatt ttcaaagcta aataaccata cactaaagcc cctatacctg
      961 ctactacaag aatcaacaat agagactgga atttgctatc ttcgtttaaa aaagcaccaa
     1021 aaatcatctt tgtgatccac gaagccaaaa gcataatcaa ggtaagaata aagatcagca
     1081 atgtcctgcg ccagacaaaa cttggtttaa agcgggctac ttcatggatt ctctttaaga
     1141 tcaaggtaca agacacagca aatccgatca ttgtcgccaa taaagggccg tatacttcaa
     1201 ataaacgaat ggctgggtat tgaagaagga gtttcactag aaacccaata accaaataat
     1261 taattgccgc cttgttttca aacattcctt gcagcatgtt ggaaactagc atatatagtc
     1321 ctaggaagag tcctacaaaa cttgcttgga tcagtacatt acttcctaga gaatccggtg
     1381 tataaaataa tgtatttagc ggataggcaa gtaacataac accaaatgtc gccggaaaca
     1441 taaaaaatga aaacaactgt aaatttccgc ttgtcaattt tgccagacct actcgatctt
     1501 tcaacgtaac agcttctgta atcaacggta acccagtaga cgcgatcgat gttgccaatg
     1561 caataactac catcgtcaac ttatctggat tggcactaaa aatcgaaaaa agatccagta
     1621 actgtgcatt ggaatactcg gtggatgcag acatgatctt cataaaagtg aactgatcga
     1681 ctaatttgaa gatcgttacc cctgaaccca caataataaa cggaatcgct tctttaacgg
     1741 tatctaacaa caattccttc gttgcaatat ttattttgtt ttcgctgtag tgaataaacg
     1801 ccgatgtata cgcttgatgt ttcttcaaaa aatacaaaag gacaagaata cttgctagca
     1861 taccgataaa tgccgcgaaa gttgattgcg tgaccgccgt cacatagtca ccatccatga
     1921 ctttcatgat gatgaatgta gccaatagca tataaaaaat acgtgccacc tgttctacaa
     1981 tctgcgacag cgcataaggc atcatttctt gattgccttg caaataccct cgtatcacac
     2041 tcatacaagg aaaaaccaat acagcgatac tcaaagaacg catcgttgga atcagttctt
     2101 ctccgccacc agaagcatga gctagccaag gagaggcgat gtacatgaac aaagcaaaca
     2161 acaggcctaa cccgcccatc atctgtaatg cacggataaa taatcgtcgt gaagtgccat
     2221 attcattcaa tgaattatag cgtgcagttt gttttgcaat cgcagcaggt attcctgctg
     2281 tagagatcag tagaaacaat gcatatatat tgtatcccat attgaataat ccattggcag
     2341 ccttagcatt ttcgcccatc caagcatacc agggaatgat atagacagcg cctagcaatc
     2401 ttgaaatgat attactggcg gtcatccaag cagagcctcg tgccattttt tcttgcggtg
     2461 ttaattgatc taatgctggt tttctttgat tatccatcgg tcagattcca actttctaca
     2521 aaacattact atcattttaa ttggtaaaac agaaactggc aatcgatttt atataacttt
     2581 tataagaaca attgtttttt tgaattcggt ttcgtttttt tggtaaagta aagaaatata
     2641 cagcctttta tggctaacat ttataggaga tatcaatgac acaagaaata aaaaaactac
     2701 ccgttatatt tttaggtctt ttctttatca cagttggatt gaattggttc ttgctccctc
     2761 acgatatagc ttccgccgga gtgggcgcca tcggacattt agttgaaacg gccttttcta
     2821 tccaacgctc agttactgtt tggagcatca atcttatgat gctgcttcta gccgctgtcc
     2881 tattaggaaa atcagtattt tttaagactg tagcgggaag tctgcttttt cctgttgttt
     2941 tagagattgt tccaaaagtg gaaatgatct cctcacattt cttttcactg atttcaggaa
     3001 gtcttttgtt cagtcttggg gtttacacat tgtacacagt cggggcctca aatggcggta
     3061 taacgatacc gccgattatt ttccacaaat tttttcggct tccaatgccg caaggacttt
     3121 tacttaccaa tagtcttatc gtctttttga attattatgt ttttggattg ttggagacac
     3181 tattcgttct cctatctatt acagtcagtt cattgtttat gaaaatactc atccgcttgt
     3241 cccctgtgtc aagaaaattt gaataaagct ggattttgtc atagcggaaa caaacgatat
     3301 attaaaaaga gtgtgctata ataaaccaag aaattgaata aagcgaggga tcaatcaatg
     3361 agcttctctt tagaagacat ccgtcattta ctattaaaag aaaatctatt aaaagaattt
     3421 gtttcaacaa gaggctggca tcttgatgta ccagaaaaag ctgaatttct tcagttaagt
     3481 tatgattcaa gaaaagctga tgcttctact ttgtttttct gtaaaggaat gaatttcaaa
     3541 gaagaatatt tggattcagc aatcgaacag ggtattcaat attatgtatc agaacaacca
     3601 tacgaaaatt cagcagtagg aatcatcgta acggatatcc gtaaagcaat ggcgcttctt
     3661 gctatggctt tttacgacta tccgcaaaat aaattgaaag tgatcggatt cactggaaca
     3721 aaaggtaaaa caactgctgc gtactttaca aaagcaatct tggatcacac aacaaataaa
     3781 aaaacagcat tgctttctac tatgaataca acgttagatg gcaaaacata tttcaaatcc
     3841 catttgacaa cgcctgagtc attagattta tatcgtatga tgtcagaagc agttgaaaat
     3901 ggtatgactc atctagtgat ggaagtttct tcacaagctt acaaaacaca acgcgtatac
     3961 ggcttgactt tagatgtcgg aatcttcttg aatatctcgc ctgaccatat cagtccgatc
     4021 gaacatccga catttgatga ctatttctat tgcaagcgac aattgatctt gaattcaaaa
     4081 acagttgttt taaatcatga aagtgactat tttgatttac ttagggaaac agccgatcta
     4141 ttccagattc caacgatcac ttatggtcgt tcggaagaaa gcaattatca agtgatccgt
     4201 tcagataaag gtactcacgg ttttacttta tcttctcatg aagatcgctt agctatctgc
     4261 gatactgctt atgatatttt gctggctgga ggattcaacc aagaaaatgc agccagtgcg
     4321 atcattgccg cagcactaac aggtgcctcc agtcaagatg cacaagacgg attaaaagaa
     4381 gctcgtgtac caggccgaat ggatcaattg atccaatcca atggagcgca tgtctatgtg
     4441 gactatgcac ataattatct aagcttgaaa acattgctga aatttgccaa aaatgaacat
     4501 ccagatggcc gtgtaattgt tgtattaggc agcccaggaa acaaagcaat ctcccgtcga
     4561 catgattttg gaaaagtgct atcagaaaca gctgacatcg cctttttgac ggcagatgac
     4621 ccagcatttg aagatccgaa aaaaatcgcg gaagaaatca atgaagcaat tactaatcca
     4681 gatttgatcg ttcattatga aatggatcgt cctgaagcga ttcgacgcgc tttagcagaa
     4741 agcgctccgc aagattcagt cgttattgcg ggaaaaggcg tggatcccta tcaaaaaatc
     4801 aatggtgtag atgaacctta tgaaggggat tatgcaatcg tgaagcggtt gattgaagaa
     4861 taatggtatt tatatactag tggtttccca tttctaagaa aaaaagggac tgtgattttt
     4921 tgtcacagtt ccttttttct tagaaatcat tttaactttt cactagctaa ttctgctgcc
     4981 ttttctcttt cttcatttaa taatttatta tcatagaatt tgataaatgg gaaccagata
     5041 atgaatgcga tgattgcaca aatgactgct aacacagcag ctttccaatc accaccacta
     5101 ccaacaaatg ctccgatccc gactggtgaa ggccacggca tctgtgcaag catcggtttg
     5161 acgatttcca gccgaatggc aaaataagcg agtgtagcag aagccatcgg agctaagaaa
     5221 aacggaaccg ctaaataggg attgtagacg attggcatcc cgaaaatgat tggttcattg
     5281 atattgaata atcctggtac aagtgaggct tttcctaaca ctttcagctg atcagatttc
     5341 gcgaggaatg caataaagat gatcaatcct aaagtcgcac ctgatccacc aacggttaca
     5401 taagaattgt tgaattctcc tgccaacgga taatttgcgc cttgtgcgtt agctgccata
     5461 ttagctaata cgatcggggt caagaaagaa gtgatgatat tcgctccatg gatccctacg
     5521 atccacaatg catggatcaa gaaataaatc accatgacac caagccatgt attcgtcaac
     5581 tgcgtaacaa acccaaatgg gatagcaatc attttgaaga tatccgttcc tagtgcaact
     5641 aataatccat tgatgatcat caccacaaac gcaatgacaa atgttgggat cagagcagtg
     5701 aaagaacgag aaactcctgg cggaacaaca tctggcattt ttatgaccca atttctcttg
     5761 acacacatac aatacaattc gaccgcaata atcgacataa taatagctgt aaagattcct
     5821 gaagttccta aacgttcgac aaaagagccc atcctaatac catttactac agtttctgtc
     5881 tcattgatag aagcaatcaa gctgatttta ccatcagtca tgatcaattc agggatacac
     5941 ataaagaatg caaaaacaga tagtaatgcc cccgtgagcg gattaacatt taatttttgt
     6001 tcatctcgtt caattgacgt taattcataa gcaaacacaa tggcaaagta aatagataaa
     6061 atccccatcg tcattgtatt gacgatcatg tacaagtcac taactttgaa aaaagttgca
     6121 ttaaaaaatc cttctaaaaa tgggaacggt aatggcaaaa tatttaaaac caaaaacatc
     6181 gagcctacaa tcgtgaaagg aatagaagcc atccccgctg ccatgacagc ccgaacgatt
     6241 ttaagttgcg caattttccc cataggtccc atcaggtatt tctgcaaaaa atcaaacatt
     6301 agttattctc ctttcctaaa tataacgctt tttcatattg tttaatacgg atatactgtt
     6361 ggtcttggtt gagtgaaatt ttctttaaac gatactgtat aaatgcactg gcaaaaatac
     6421 cgcttacaac aagaatgaaa acaaataact gtcctccatt gttatttgcc ataaaaggat
     6481 acaagaatgt aaatcctgag gtaaacaaca ctggaatcac taataaatta aagacactct
     6541 ggaacaaaaa ataccacttt gtccatggaa cgttcgtttg gtgttttcga taaagcttta
     6601 cttgttctcc tactgcaggc aaaatagcta aaatcagaat catcggtaaa ctacttccta
     6661 aacttttagc tgatagaagc agaatcatcc attgcatgtt catgaaaaag aaaaaggcag
     6721 tcaggtaacg tacgattaga taacgattga aatacatgtt tttcaaacta agttgctttt
     6781 tttctaattc gattttcctt tttactgtat cctgcacaat gatccttcct ttctatttat
     6841 ttttttgata caaataattc atttcctgtg caacttccaa taatgtcatc gtcgtcatca
     6901 gatgatcttg tgcatggacc atgataattt ccatttctat ttttgtcccg ctggcatatt
     6961 ctttcagaag ttgagtttgt gactgatgcg catgaagaat ctcttcattt gcttgtcgca
     7021 acttctcttc tacttcttca aacatctctt tacgcatcga gcgaaatgct tcatgtatca
     7081 gtgtccgtgc atttccgcta tggaaaatga tatcaaaaga agtcacttgt agtgtttcac
     7141 tgtttatttt ttcagccatc tgttttcctc cttattcgat cagtttgata aatttactgc
     7201 tgaaattctc aaaaataggt tcagctaaga tttcctgctg tacttttagc tggtcaacta
     7261 acctaacgat tgctttagtc acgaccgtaa ttccttcatt ttcgacataa gaaggcgaaa
     7321 ctaaaaaaac aaaatgaatt tcttgatatt gatcccactg catcccagaa ggaatcaatg
     7381 caacggctac ctttgtttcg attccaactg gaacagcggg atgtgggact gccactgtat
     7441 cactgaatat gatttgtccc atattttcac gttgctggat ctgctttcgc atttctctta
     7501 tatatccttc ttcttcattt atgctcagct tttgcagcaa atcatgaaga atttcttcct
     7561 ttttcggttc gtacgtataa gtcttgaaaa atttttcaga caactgttcg gtgtaaatct
     7621 gctttttttg ctgtagagaa aatgctggta tcggtctatc tgaggaatga tagggtatag
     7681 attctccaac tacctttcgt attttacgaa catcatcttc attcaaaaaa atactgacat
     7741 gtaaaactgg aattttgaaa aacattgtcg aaagatcaat agaagaaata atcaggtcga
     7801 ttccttttaa agttgtttca ttgatctcat aatagccttt tacacttacc actgtaatat
     7861 ttttaccgaa ttcactgact acgcgattct ttaatagttg ggcactgccg taacccgttg
     7921 cacagataat caatgcatgt agtttatgcg cagctctttc tttttccaat gccgccatga
     7981 gatgcaaagc caaataggcc cattcatctt cattgatggt atagtttttc aaagccggca
     8041 tgttgctgaa atgccttttt actgcgtcaa agactttggg attttctttc tttatctcct
     8101 cagtcaatgg gttctcaatg cgaatacctc ggctcaatcg aatcatcatc ggtgacaaat
     8161 gatcgagtag tccattgaaa agctggagat cctctactaa tgaaaaatga aattcctgag
     8221 acaatctttt cagaactttc gctaattcct ttgatagttc ctgatcattt tgattttctt
     8281 tgtgattcga cttagccatg agatgtaatg tcaaataagc aatttcttct tcgggaaacg
     8341 atacacctgc gattacctca atacgttgaa cgatccgctg cgccacttga tactctactc
     8401 gcttagaaac ttcttgttcg atgcctaatt ccttgatttc aaacccttct cggagccgtt
     8461 tgatccccaa agccaagtgt aaagtcagat tctgtatgat aaaatccgaa acttttaact
     8521 tagcttctct tgtttcatcg agaatgataa tgactagctc ttcaaaactg atgtctggga
     8581 aaaaacggct ttgccctaaa tattctttca atgaatttcc ataatggttt ccaaaaaaag
     8641 tatccatgat aaaatgccgt cgatttcttt cttccccttc gatccatgtc ccttgaccat
     8701 gcttagaaac gatttttagt gaatatggaa tcaatcgctc cttgatttct tgcatgtcct
     8761 ttgtcaaact ggaacgactg atataaagct cttctgccaa atcatccata aaaagaatgg
     8821 catcttccaa aagcaattgg ttcaatatat acttttgtcg atcttcggac tcataaaatc
     8881 cttgcggttc tctagcatat ccaggtacta tttcttgccg actcaaaaac aagtcaaatg
     8941 tcagtctttg taaaatatgc agttggtagc cataacctgg tttagcaatg atcttacctc
     9001 cgtttttttc tactacctct ttgatccgct gcaagtaatt acggattgtc cgatcagata
     9061 ataacaattc agaagctaaa cgttgactgg tgacatactc cttcttgtga tcaatcaaat
     9121 atctcaacag caatttttcc ttctgtttca ttatcatcca ctcctattcc atcatatgtt
     9181 ctcttcgatc acctctgcta acttctggat gcctgtaggg attgggacat acgcttggaa
     9241 agggatacta acaacaggct ttccaacaga ttctcctaat tttttgaatt tatccaaaaa
     9301 catcgtcgtt tgcgggctga tcagaaataa atcaaaatca ctattcttaa tcatattatc
     9361 gccttctgta gctgaaactg catctacgag tacctcttct cttttttgct taaagaaatc
     9421 cgttgtcttt gaggccatca tcgaggatga cattcctgcc gcacaaataa tcaatgcttt
     9481 tttcataaat caatcactcc ttttattttt ataaaccaag catatcttgt aagcgttttc
     9541 tattcaaaat atatatttcc gtagcattgc ggaaaaaatg tgtatcatat cttgaataat
     9601 aatttctggc accgtgatat actagtgata gaattttttc aggaggggat ttagtgagta
     9661 tcacagtgaa tattttgtat acaagtacaa atggacaagc cagaaaattt gcagaagaaa
     9721 tggtcgaaag aaacattgtc aaagcgattc gtgcagaaga aggaaacgag aaatatgact
     9781 attactttcc actagaagat ccagaatctc ttttactgat cgaccgttgg aaagatgaag
     9841 aagcaatcga aaagcatcac aaatccgaaa tgatggctca aataaacgaa ctccgtaaaa
     9901 agtataaatt aaaaatgaaa gtcgaacgtt tcactgattt accgtaaaaa gatacacatg
     9961 gaatgatagc aatttatcat ccatgtgtat cttcactttt tccttatttt ttattcgatc
    10021 tcatctaatg attcaaaccg tgtttttctc ttatttttcg gataagtatt tcagtcaccc
    10081 caatatacat gattcctaaa cataccccaa ggatattctc aaaaatgcgg atcgttactg
    10141 ctcctggaac cccgaataaa ctggcagtga ttgccaaagc accaaaagaa ttaaaaactg
    10201 ttttatatct ataggtagaa caaacaccta aagctagacc acctaatata ccgacccagt
    10261 taaaaggaat aaattggctg ataccaataa ataaaacaca tccaatcaat gagccaatga
    10321 ttcggtctac tgctcgttct ttgaaaccaa tcgacatcaa tccatagcta gatacaatag
    10381 aagaaaatgc aaaccctgcc cacatcaatc gctgaaaagg gacatactct cctgcaacga
    10441 tcaaaagact aatgccaaat gcatagatga gcatccaaat tttttcctgc gaatacatgc
    10501 ctttcaaaaa aatctcttta aacaagcttt tgtctttgtt cttttctcga tgttttctgt
    10561 aaaggagaac tgaaaaccaa caaaaaaaca aaaataataa gctacttgtt tgtgtaaaga
    10621 aattcttgtt caacgaatct ttaggtaacg aataaacaat aaagagatat gaaaagccgt
    10681 acaaagaagc attccccatt ttgggatcac tagcggttgc cattaatatg ctgcttagta
    10741 aaataaaatg aatcaacatg cccataactg accatttgat cagctggaca tacggtgcta
    10801 ctaatagtga gaacatgacg attgctaaac ctatgatcga atgggaaatc ttataaccaa
    10861 agtcaacaaa acgaatactc atcaacatac agaataaaac cacagcgtat ggtttatgct
    10921 tcgattcaaa taatgttgta attataatga taaaacaaat ggcaaacaat acgatgaata
    10981 atgatcgtaa aaacaatgaa caaatataca tgcacttttc ttttttagaa gctgcttctc
    11041 gtattttttg ttttaaaata aacggatcta gttgtagaga ctgataaaaa tccacctgtt
    11101 gtcgtccttt ctttttactt ttatttttta cagtcatcta tgacaggttg taaatactat
    11161 ggaacatcat atggcgttgt cattttcttg tcaactattt ttcctattca tgtttttata
    11221 aaaaaacata tcgttttctt gtatgattaa atcaccacaa aataaacaac tgagagaacg
    11281 atatgcttta tgaatggttc tatcaaaaaa attctgagat caatagacaa aaatttgatg
    11341 attacagagg tctcttacga aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt
    11401 ctcttccctg ctccatgtgt ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg
    11461 aaattaatgg tggtgaaaaa caagaagaag gaaaccattg tccactttga ataatataat
    11521 catatgccaa tggttatacg cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc
    11581 cattggaacg ctcaaagtta ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga
    11641 cataaaatca cttctttact gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt
    11701 taggtatctt tgacaaccgt tattcgcaca ttgaaagagt tgagaagcta tttaccaaag
    11761 tcttccaaaa gaattctgtc ttgagtgttg agggttgtga atttcgttct cacgtttccg
    11821 ttgaggataa actgagtttt atttccgcgg atggcgagac agaaaaatta atcgagaaaa
    11881 tttacctaca caaaaaattc ctcgattaac aaactatttc cttagctgca ctaatcctaa
    11941 agaaatagaa ttcttagtta cagacatgaa cgccacctac tttcagctca ccaaacatgt
    12001 tctaccaaat gcgaaattgg tgattgatcg attacttggt ttttccacgc ctttcaagga
    12061 ggcgtaacct ttattcacga gttggttgaa gctttccgag acaaaagacc cgacttgttt
    12121 ttctccttat tgacaaagag ccattaaaac tagagaaaaa attacaactg atcgctgatt
    12181 atttgtagaa agaaaatcaa agtattaccc aataaacttg agtcattagg caaaaaacac
    12241 tgcgaaactc aagaatgatt ttcgcagtgt ttttgtatat tttttattaa ctagtattac
    12301 tacaaaggtt tatttttcac ttctcaacgc agcacaaaat tgtctttact tatttactgg
    12361 ttgggttcaa ccttctctac tccgatacta atatttgttt gtatacccct tctaaaatcg
    12421 gtagtttcca ttcaattgat gggtactagt tgattttaaa aactatgctt attcaatttt
    12481 ttattttagc tcatatattt cgtttctttc taaattttat actgatgaaa acaagtagca
    12541 cacccaatat tgacaatata aactggctaa tgatggtttc tccagttttt ggaagatgct
    12601 tatttccact gcttgttgga ttgtttcctc ttatatctga gctatcagca acttttctat
    12661 tctcttttgt taaaaagatc gccacttctt gttctttttc gatcttgaaa gttactggtg
    12721 tcgcatc
//
LOCUS       DACEMO010000068        12703 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEMO010000068 DACEMO010000000
VERSION     DACEMO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.069"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            29..1912
                     /locus_tag="IW728_001464"
     CDS             29..1912
                     /locus_tag="IW728_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1024831.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(1987..2271)
                     /locus_tag="IW728_001465"
     CDS             complement(1987..2271)
                     /locus_tag="IW728_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1024832.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2261..2995)
                     /locus_tag="IW728_001466"
     CDS             complement(2261..2995)
                     /locus_tag="IW728_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1024833.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3124..3747
                     /locus_tag="IW728_001467"
     CDS             3124..3747
                     /locus_tag="IW728_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1024834.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3939..5060
                     /locus_tag="IW728_001468"
     CDS             3939..5060
                     /locus_tag="IW728_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1024835.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5067..8195
                     /locus_tag="IW728_001469"
     CDS             5067..8195
                     /locus_tag="IW728_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1024836.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8214..11408
                     /locus_tag="IW728_001470"
     CDS             8214..11408
                     /locus_tag="IW728_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1024837.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11554..12261)
                     /locus_tag="IW728_001471"
     CDS             complement(11554..12261)
                     /locus_tag="IW728_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1024838.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4339 a         2225 c         2659 g         3480 t
ORIGIN      
        1 aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg
       61 ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca
      121 ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc
      181 tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat
      241 ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt
      301 gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt
      361 tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta
      421 tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg
      481 tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag
      541 aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat
      601 cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt
      661 ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa
      721 catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact
      781 gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct
      841 cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt
      901 gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta
      961 tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca
     1021 tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg aactttctta
     1081 tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa
     1141 aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt
     1201 acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga
     1261 tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa
     1321 caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc
     1381 caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat
     1441 gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt
     1501 cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt
     1561 gaaagacact gttctgatcg gactaggaac gaatggctca ttctcggaag ctcagtttga
     1621 tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac
     1681 gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa
     1741 tatgacgctg attgattggt acagctacag taatgaccat agtgactggt tttatgatga
     1801 tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt
     1861 gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca
     1921 aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt
     1981 taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt
     2041 ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga
     2101 aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc
     2161 accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata
     2221 aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct
     2281 cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt
     2341 tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc
     2401 caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct
     2461 gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta
     2521 gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga
     2581 ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat
     2641 tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga
     2701 tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt
     2761 ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa
     2821 aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac
     2881 gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga
     2941 taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt
     3001 ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag
     3061 gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag
     3121 actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga
     3181 aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct
     3241 ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc
     3301 agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga
     3361 tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta
     3421 aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct
     3481 acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca
     3541 gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg
     3601 cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg
     3661 gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat
     3721 ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa
     3781 tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg
     3841 ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt
     3901 gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg
     3961 attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac
     4021 tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag
     4081 acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg
     4141 tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca
     4201 gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac
     4261 agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt
     4321 actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc
     4381 aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag
     4441 tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg
     4501 aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac
     4561 taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc
     4621 cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga
     4681 caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa
     4741 cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt
     4801 acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc
     4861 aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc
     4921 gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg
     4981 aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag
     5041 aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg
     5101 gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga
     5161 tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt
     5221 ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac
     5281 cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg
     5341 aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag
     5401 ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac
     5461 gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc
     5521 aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata
     5581 aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat
     5641 taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc
     5701 ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg
     5761 ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag
     5821 aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg
     5881 aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag
     5941 ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc
     6001 aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt
     6061 cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt
     6121 ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa
     6181 aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag
     6241 acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc
     6301 aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg
     6361 aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc
     6421 aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac
     6481 tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac
     6541 aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt
     6601 tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa
     6661 aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg
     6721 agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc
     6781 ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg
     6841 aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa
     6901 aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg
     6961 ccaatgcaag aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat
     7021 tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag
     7081 aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga
     7141 tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag
     7201 caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg
     7261 aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt
     7321 tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag
     7381 cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag
     7441 ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag
     7501 agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat
     7561 acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt
     7621 atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat
     7681 tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc
     7741 aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat
     7801 ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct
     7861 atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta
     7921 ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg
     7981 tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg
     8041 aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta
     8101 gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta
     8161 gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat
     8221 ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt
     8281 tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat
     8341 tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt
     8401 gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa
     8461 atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg
     8521 agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg
     8581 tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag
     8641 agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat
     8701 atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg
     8761 ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa
     8821 tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac
     8881 aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc
     8941 tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc
     9001 aaggaataca attttccgat caaaacaaac ctttatcatt caaagtggaa aaatttggag
     9061 aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg
     9121 gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt
     9181 ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac
     9241 cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc
     9301 ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag
     9361 caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg cgaagtcatc tattcgactg
     9421 acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag
     9481 agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg
     9541 aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct
     9601 tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc
     9661 ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg
     9721 atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg
     9781 atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac
     9841 aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc
     9901 atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt
     9961 ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac
    10021 agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt
    10081 tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga
    10141 aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg
    10201 ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg
    10261 ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg
    10321 ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt
    10381 atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca
    10441 gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg
    10501 ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag
    10561 gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga
    10621 ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa
    10681 cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact
    10741 tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga
    10801 tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta
    10861 ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg
    10921 ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct
    10981 tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac
    11041 cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga
    11101 tttccttaaa ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt
    11161 atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg
    11221 gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca
    11281 tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag
    11341 aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg
    11401 aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg
    11461 attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc
    11521 ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc
    11581 taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg
    11641 ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt
    11701 gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc
    11761 tagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt
    11821 gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt
    11881 cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt
    11941 tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa
    12001 gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg
    12061 atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc
    12121 actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac
    12181 gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc
    12241 aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca
    12301 aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc
    12361 ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa
    12421 aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat
    12481 tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc
    12541 tcaatataac tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt
    12601 tatttagtaa tcattgcttt aaaatactac gcttatataa cagagaaaat aagcgttata
    12661 ataaagaaca gtattattta aaatgacaga aaacattact att
//
LOCUS       DACEMO010000069        12673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEMO010000069 DACEMO010000000
VERSION     DACEMO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.070"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(277..639)
                     /locus_tag="IW728_001472"
     CDS             complement(277..639)
                     /locus_tag="IW728_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024839.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(713..1093)
                     /locus_tag="IW728_001473"
     CDS             complement(713..1093)
                     /locus_tag="IW728_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1024840.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1175..2350
                     /locus_tag="IW728_001474"
     CDS             1175..2350
                     /locus_tag="IW728_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1024841.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2427..3011)
                     /locus_tag="IW728_001475"
     CDS             complement(2427..3011)
                     /locus_tag="IW728_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1024842.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3212..4210
                     /locus_tag="IW728_001476"
     CDS             3212..4210
                     /locus_tag="IW728_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1024843.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4322..4831
                     /locus_tag="IW728_001477"
     CDS             4322..4831
                     /locus_tag="IW728_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1024844.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4861..5520)
                     /locus_tag="IW728_001478"
     CDS             complement(4861..5520)
                     /locus_tag="IW728_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1024845.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5522..6286)
                     /locus_tag="IW728_001479"
     CDS             complement(5522..6286)
                     /locus_tag="IW728_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1024846.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6294..6971)
                     /locus_tag="IW728_001480"
     CDS             complement(6294..6971)
                     /locus_tag="IW728_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1024847.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7008..8393)
                     /locus_tag="IW728_001481"
     CDS             complement(7008..8393)
                     /locus_tag="IW728_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1024848.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8603..8687)
                     /gene="ffs"
                     /locus_tag="IW728_001482"
     ncRNA           complement(8603..8687)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW728_001482"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="IW728_001483"
     CDS             complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="IW728_001483"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1024849.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9332..10189
                     /locus_tag="IW728_001484"
     CDS             9332..10189
                     /locus_tag="IW728_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1024850.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10270..10605)
                     /locus_tag="IW728_001485"
     CDS             complement(10270..10605)
                     /locus_tag="IW728_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024851.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10672..12042)
                     /locus_tag="IW728_001486"
     CDS             complement(10672..12042)
                     /locus_tag="IW728_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1024852.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12120..12656)
                     /locus_tag="IW728_001487"
     CDS             complement(12120..12656)
                     /locus_tag="IW728_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1024853.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3763 a         2574 c         2119 g         4217 t
ORIGIN      
        1 tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt tatcatgtat
       61 ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt cgatcaatta
      121 ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca ctaaaaaact
      181 atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa gaagtttgaa
      241 aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca ctccctctac
      301 ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat atccttcgcc
      361 taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa cattttcctc
      421 tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga tatgaactat
      481 ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa gttccgctaa
      541 cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac catcctccaa
      601 atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct cgatcaactt
      661 gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact ttttatttct
      721 tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt aactgctccg
      781 caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt ctgcgatatg
      841 gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt ttggaatatt
      901 catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat gaatggatat
      961 ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc tcgttatcaa
     1021 gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt atttgtccaa
     1081 ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa atagctcaaa
     1141 aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata aacagatttc
     1201 aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc aaatggatga
     1261 cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg tagctaaagc
     1321 gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg ataaactata
     1381 tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga aagaaaatat
     1441 cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt ttacaaaacc
     1501 aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta ttatagaaac
     1561 aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt tcatcatgga
     1621 cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga ttttatgcaa
     1681 tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt taagcgaatt
     1741 gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt taactttatt
     1801 cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc tactagttgc
     1861 ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa tggtatttat
     1921 caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc aacagcacga
     1981 aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg gagaatggtt
     2041 aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt tttttgaaca
     2101 gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt ggttagattt
     2161 taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga aaggaaaagt
     2221 cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc gcttgaatat
     2281 tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg ctttagagac
     2341 aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt tcgtcctttt
     2401 tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc aatcactaca
     2461 tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc tactgtgtcc
     2521 atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg agttccgcca
     2581 cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg tccaagcata
     2641 ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt gtttggacct
     2701 gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa ttcatcttca
     2761 aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc gccgccttgg
     2821 atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc ttttttggaa
     2881 agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg aacagtaatg
     2941 tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa atctagttgt
     3001 gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa taacataata
     3061 cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac tagatacatt
     3121 ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc tcaattaaat
     3181 tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta caatcatcgg
     3241 tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag ctaaaacaaa
     3301 aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc ccgaaaaata
     3361 tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg atcaattaga
     3421 aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc tctcgcttac
     3481 tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt ccaaagcagt
     3541 cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag ataatgctga
     3601 atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt atgctggaaa
     3661 aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga tgttagagcc
     3721 tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc acgaacatag
     3781 tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca tcgatggatt
     3841 atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa gtgacgagac
     3901 gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta atttagaaca
     3961 tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat cagatatgtc
     4021 tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag gaaaagtgaa
     4081 actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt tacactatat
     4141 taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata aaaatcttcg
     4201 tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc agtttcttgc
     4261 atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg ggagcaataa
     4321 tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga aggaacgtgg
     4381 cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact atatttctga
     4441 tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc gtgaagtaca
     4501 taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa aattgatgag
     4561 tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg agatcctagc
     4621 cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt atatcgacaa
     4681 ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg tccatacctt
     4741 cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag cacacaaaga
     4801 accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga aaacgtctat
     4861 ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga aaagaaaaac
     4921 agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga aaaattcttc
     4981 tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat cgttgttgaa
     5041 aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga aaaaaccgaa
     5101 aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa gccgatcatt
     5161 cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa acacagcata
     5221 atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag cagaaactgg
     5281 gaaatctggc aagctgagga tggttttgaa gggattattg agataattca ttaggtgatc
     5341 aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt gcaaatgtcc
     5401 gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca gactgatcag
     5461 tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg aatttttcat
     5521 attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata ggcaacccag
     5581 gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata ttattgcgta
     5641 tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct tctttttgca
     5701 gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg atgtagatcg
     5761 gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca ggaagaaggc
     5821 cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca cctttttgaa
     5881 tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag cctaccacta
     5941 catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag tcaatcaatc
     6001 ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata aaatcgattg
     6061 ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac tcatctgcta
     6121 gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac aagaaaggta
     6181 attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt tgtgtcccac
     6241 gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat ccactactct
     6301 tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg ttgttcttgt
     6361 ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat aaccggtttt
     6421 ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt atggtttttt
     6481 ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc gatgacaaaa
     6541 tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc gattcgctga
     6601 tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag ttgttcataa
     6661 ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg ttctccaagc
     6721 ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct ttcacctatc
     6781 aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc tgctttaggc
     6841 tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt ttcctgttca
     6901 gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg ttcattttgt
     6961 tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta tttgattggg
     7021 tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa caaaaattca
     7081 ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg atctacggta
     7141 gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac ttgatggtta
     7201 atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc gcgaaatccc
     7261 attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc taaaactaag
     7321 cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg cggcaacgca
     7381 gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa cagaccactg
     7441 tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc tacttggata
     7501 aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc tacttctttt
     7561 tcttttaata attcatgccc acgttctaga taactattta tttcttgttc atcttctgta
     7621 aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat ttttttctct
     7681 tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc ggctgctatt
     7741 tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga tcctaaaata
     7801 acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc gagatgactc
     7861 atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg caaaatatca
     7921 taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg agcagtaaat
     7981 gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg tttcgtccat
     8041 gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc aaactcagca
     8101 tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt gtttcctatc
     8161 gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc attcgttgct
     8221 tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat cgttgtcgtt
     8281 ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat ccttggccaa
     8341 ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc catgtaccct
     8401 ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg aaacaagctc
     8461 tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc gataaatcag
     8521 tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa aaaaacagac
     8581 ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc ttagtgctgc
     8641 ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt cggcaaagaa
     8701 tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt ttcgctggcg
     8761 gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa tgccaccttc
     8821 tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg taccggctgt
     8881 tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca aaatcatcgc
     8941 gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt ccaagcgcca
     9001 gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg tagtagcatc
     9061 ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca atacaaccac
     9121 tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca atgcaaggcg
     9181 catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc cttccctgcc
     9241 tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa cttttttttc
     9301 tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac ttgctcttta
     9361 cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt cttttgcaat
     9421 tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa ccagcctttt
     9481 gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc gctatctttt
     9541 taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc acgtcgaatc
     9601 aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat ttcgctattc
     9661 tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa taaaccgtga
     9721 ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg attttgatac
     9781 gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg tccctttgtt
     9841 tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc acagccgtac
     9901 tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa aagatagtaa
     9961 gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga tcattcaaga
    10021 attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc ttcatcgtaa
    10081 tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc taaaaaaagc
    10141 caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa gatagattaa
    10201 aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt gttcaaaagc
    10261 tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta catcttcggt
    10321 tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt tgatttttgc
    10381 taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga tttctagtaa
    10441 tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc gagtagtcat
    10501 atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta cactggcagc
    10561 ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta tcagctcctt
    10621 tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca ctcagtgaaa
    10681 tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc caaatccgac
    10741 aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt gaaatgtttc
    10801 tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta tattctctat
    10861 cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt tgaaatctcc
    10921 taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta actcaggaac
    10981 ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat gaaaatcatt
    11041 tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac ttttttttcg
    11101 tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg caacaaaatg
    11161 tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac cagctacgct
    11221 tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct caatcgttac
    11281 tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg caaacgtaca
    11341 agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag acaaaatctc
    11401 ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta ttgcatttgg
    11461 atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc gatcaaaaaa
    11521 gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg cgttcgaaac
    11581 tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga tctcattcgt
    11641 cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac caccgggagc
    11701 cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc caacgatcat
    11761 tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact ttccattgac
    11821 agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc tatggtaagt
    11881 ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt ttttcacgct
    11941 tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa gacgatattt
    12001 tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt ttcgttagac
    12061 attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca tgttgctgcc
    12121 taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc gtttttatcc
    12181 caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg caaacttccc
    12241 aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt gtgacctttg
    12301 acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga ttttgcaatg
    12361 atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac aatgcttaaa
    12421 acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat ttcttctaat
    12481 atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta tgtagaacaa
    12541 ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc ttccacgtat
    12601 accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc catcattctt
    12661 acctccgtct ctt
//
LOCUS       DACEMO010000070        12500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEMO010000070 DACEMO010000000
VERSION     DACEMO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.071"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            333..1961
                     /gene="treC"
                     /locus_tag="IW728_001488"
     CDS             333..1961
                     /gene="treC"
                     /locus_tag="IW728_001488"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1024854.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(2021..3202)
                     /locus_tag="IW728_001489"
     CDS             complement(2021..3202)
                     /locus_tag="IW728_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1024855.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            3454..4977
                     /locus_tag="IW728_001490"
     CDS             3454..4977
                     /locus_tag="IW728_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1024856.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(5046..5774)
                     /locus_tag="IW728_001491"
     CDS             complement(5046..5774)
                     /locus_tag="IW728_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1024857.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(5787..7067)
                     /locus_tag="IW728_001492"
     CDS             complement(5787..7067)
                     /locus_tag="IW728_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1024858.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(7208..7810)
                     /locus_tag="IW728_001493"
     CDS             complement(7208..7810)
                     /locus_tag="IW728_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1024859.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            8055..8261
                     /locus_tag="IW728_001494"
     CDS             8055..8261
                     /locus_tag="IW728_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024860.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            8573..9292
                     /locus_tag="IW728_001495"
     CDS             8573..9292
                     /locus_tag="IW728_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1024861.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(9373..10056)
                     /locus_tag="IW728_001496"
     CDS             complement(9373..10056)
                     /locus_tag="IW728_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1024862.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            10274..12460
                     /locus_tag="IW728_001497"
     CDS             10274..12460
                     /locus_tag="IW728_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1024863.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         4522 a         2136 c         2404 g         3438 t
ORIGIN      
        1 tgaatcagag gttgtaaaaa agccgtcctg catcgagcaa aaagaatact caaataaaat
       61 tagctcctca tgttttacat ttgagaagac tgattgccgt agataaaagc ttttgaacac
      121 catttataga agaaacaaga atattttgct attttaaaca agaggataga caaatacatg
      181 tctgatctct tgttttttat tgggcaacag acctcttaga atgtataaag ataaatttta
      241 caaacgcttt cggttcagtt ataataataa atgataactt gtatatataa gttgtggtag
      301 gtaataaatc agttgaatgg aggtaaatat ccatgacatt taaagacaaa gtcatttacc
      361 aaatctatcc caaatcttac aaagacagta atggtgatgg aattggagat ttaaaaggaa
      421 tcaaagaaaa actcccgtat ctgaatgatc taggcatcga catgatttgg ctaaacccta
      481 tttatccaag ccctcaaaaa gacaacggtt atgatatctc tgattacaca gccatcgatc
      541 ctatctttgg tacgatggag gaattcgaac agctaatagc cgaagcaaag aaataccgga
      601 tagaaatcat gctcgatatg gtgctgaatc acgtttcgac agaacatgaa tggttcaaaa
      661 aagcactagc aggggaccag cattatcagg acttcttcat tttaagagaa gagccaacgg
      721 attgggtatc aaaatttggt ggaagtgctt ggtcgccatt tggagatacg gggaaatatt
      781 atttgcactt atatgatcgg acacaagctg acttgaactg gcggaaccaa gcagtaagag
      841 aggaactatt caaagttgtt cgtttttgga tggaaaaagg ggtaaaagga ttccgtttcg
      901 atgttatcaa tgttatcggt aaagacgaac aattgaaaaa taataaagaa aatgatggca
      961 aacctgaata tacggacaaa ccaatcaccc atacgtatct aaaagaattg aaccaagcaa
     1021 cgtttgggac ggattccact atcatcacag tcggtgaaat gagctctacg acgatcgaaa
     1081 attgtatctt gtatacagaa cctagtagag aagaattatc aatggtcttt aatttccatc
     1141 atttgaaagt cgattatgaa aatgggaata aatggtctac tcctccattt gattttgcag
     1201 aattaaagcg actatttcat acatggggag aaaagatgag tgagggtaac ggctggaatg
     1261 ctctattttt taataatcat gatcagccac gtgcgctcaa tcgatttgta gctatcgatc
     1321 agtatcgaaa acaaggagca gagatgctag caaccatgat ccatttgaac cgtgggacgc
     1381 cctatattta tatgggagaa gaaatcggca tgatcgaccc agactattca tcgatttccg
     1441 actatattga cgtagaaagt ttgaatgctt ataagttact ccttgaacaa ggattcacag
     1501 aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga taactctcga acacccatgc
     1561 aatggtcaga cacagaacaa gcaggattta ctacagggaa accttggtta aaagttggag
     1621 gcaagctaga agagattaat gtagaaaaag aactatcttc tgaagattct attttgtcct
     1681 attacaagaa attgattcgg ctgagaaaaa catatccaat cgttgcacaa ggagattatc
     1741 atgcttatga agcaaatcat ccggaagttt atggctttct tcgccaattt gaaggacagc
     1801 agctgcttgt attaacaaat ttctatgata aagatacaag gatcgccatc ccagaaggat
     1861 tcacagaagc caatattctt atcgataatt attccgatac tgttcttcat caaagtctgg
     1921 agctaaaacc ataccaggca attgctttgc tgaaaaatta aaacaaaaac acaaaacgtc
     1981 gttcctacga gctattctcg taagatcgac gttttgtgtt ttatttaaaa taatcttctt
     2041 cagaaaatga agagttttca tactctcctt tatcgccata aaatttattg tagcgttgtt
     2101 tgaatttacg gaaaataaaa gtaacgagta atttgactaa cgtatagata ggaataccaa
     2161 aaataacgcc cacaattccc attagatcac ccatgacaag taaaacgatt aagatcgtaa
     2221 tcggatggat ctgtaaacga tcacccatta ctcgaggaat caccaagtca ccgtggagaa
     2281 gttgtacggc aaaccagaca atgacaaatt tgacagccat aaaagtagag tcttggaatg
     2341 caatgatcaa acctggaaag aaggcgataa acggcccaat atatggaatg atacataaca
     2401 acccagcaga aatagctaaa acactagcaa attctaatcc gatcagtaag aaacaaaccc
     2461 agtagactgc tccaaggata aatgaagcaa tgatttgccc ctttagaaat gaacccaatt
     2521 gttgattagc aatctttgtc aaatttttaa aatctgtacg aaatgctggt gggacaatcg
     2581 ctaaaactga ctgataaaat ttttgaggat ctctcaaaag gaaaaaagcg acaattggac
     2641 cagtaaataa agtaataaaa atagctgtaa cagttgaaaa aatgttgctg atccgttgtg
     2701 cgccactttc ccaataattt ccaataaatc cagctatatc agacgtcacg tcgtcaatag
     2761 aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt ctccaaaaat tgtcggagac
     2821 tctcttgaaa atcttgaaac aaatctggca aactactgat caatgtcttt gcctgttctt
     2881 caagaaaagg aataagccat atgcttccaa gccccaataa aaataaaata atcacataaa
     2941 cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa acgtttgatg actggttcca
     3001 aaagataata caagatcagt gcaaacaata atgggggcaa gatagtagcg ataatgattc
     3061 cgacaggctt gaatataaac gagatctgat ggaaaaaata aatgatcagc gcaagtaaga
     3121 taactattcc taaaacatag taagaggttt ttcctcctaa aaagcggatc aaacgattcg
     3181 ttgtatctgt ttcttttttc actctttttc ctcctttatc tataaattca ttatagacaa
     3241 aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa aatatcgtca atcattcata
     3301 taaactttct catcaattaa aataagaaaa cgcttgacag ttcttttaac catatgataa
     3361 attaaatcct gttgctaaaa cgtaactatt acggtttgcg aaaagcgaat cggaacattc
     3421 aaatgaaaga agatgagaag aggagtgtca taaatgaaac gattgatagg tatattggcc
     3481 atgctggttt tagcaggtat gttaactgct tgcggggcaa gtgggaaagc cgaggacagt
     3541 aaagaaaaat tgagcgtgat gacgaccttt tatcccatgt atgattttac aaaagcaatc
     3601 gtcggggatg aaggagaagt agagttattg attccagcag ggactgattc ccacgattat
     3661 gaaccttctg ctaaagacat ggcaaagatc caagatacag atatctttgt ttataatgat
     3721 gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat tacaagaagg caatgtccat
     3781 acgataaaag cgacagaagg tatgctttta cttccgggaa gtgaagaagg acatgatcat
     3841 gaccatgaac acggggaaga aggccataca catgagctag acccacatgt atggctagct
     3901 ccaagtttag caatcaagca ggtagcaaac atacgtgatc agctgattga agcttaccca
     3961 gaaaaacagg aagtatggac aaaaaatgca gcagcctata ccgagaaact tcaagcactc
     4021 catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa gatcttttgt cacgcaacat
     4081 gctgcattca actatctagc gttagaatat ggattgaatc aagtatcgat tgctggtctt
     4141 tcttctagtg aagaaccgtc tgcggcgaga attgctgaac tgaaacactt tgttaaagaa
     4201 catggaatca actatattta ttttgaagaa aacgccaaag atagtattgc cagaacgtta
     4261 gcaaacgaag caggtgtatc tttagaagta ttgaatcctt tggaaggatt gacgaatgaa
     4321 caaatagaaa atggagaaaa ctatctttct atcatggaag caaacctcga agcgttgaaa
     4381 aaaacgacag agacagaaaa cccgctagaa gatactttga cccccaaaaa agaaaagagc
     4441 gtctacaacg gttattttga agacgaggat gtacaagatc gctctctttc agattgggca
     4501 ggaacttggc aaacagtaga tacgtatgta gaagatggga cttttgatcc tgtttttgaa
     4561 tataaagaaa aattgaatca ggataagaca gcacaagaat ataaagaata ttatacgaag
     4621 ggctatcaaa cagatattgc ttcaatcaag atcagtgaga aagagatggc atttacgttt
     4681 aagaatggaa caacgaaacg ttcaaattat cgttatgtag ggaaagaaat cttgacctac
     4741 gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag aagataagga aagtggcgct
     4801 tttagatata ttcaattcag tgatcacacg attgcaccgg ctgactccga acattttcat
     4861 ttatatgcag gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca
     4921 ttttatccag atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag
     4981 gcaaaaaaga accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt
     5041 tctttttatg aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt
     5101 gccaacgtga cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca
     5161 taggatttat gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca
     5221 tagaattgta attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa
     5281 gtagaaaaat attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt
     5341 ttgaagatat ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata
     5401 tgctcgacat tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg
     5461 atgttgcttt ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt
     5521 cctaaaggat ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct
     5581 tctagttcga tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg
     5641 agaagttctt ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg
     5701 attctatttt tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata
     5761 taaatgggaa tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa
     5821 cgctggtttt caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc
     5881 aagataaaca agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt
     5941 ttcaactggt ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa
     6001 tgattcttct tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca
     6061 atcgcattgt ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc
     6121 agttcgattg gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg
     6181 agttttaatg acagtaaact acggatctca ggaactttca tattatccaa catacttaga
     6241 ttggccggaa aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac
     6301 cgtaatcctt ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg
     6361 taatcatgtt tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc
     6421 tgttgcatcc tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc
     6481 caataataaa ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc
     6541 aaagctaaga catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat
     6601 gtaaataata tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa
     6661 gtctctaacg atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca
     6721 atgtgaaaat actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa
     6781 tcgccgatgc ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg
     6841 taaaccaatt gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata
     6901 gcaattagag cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa
     6961 atcagaatga ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata
     7021 caaattgtgt aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc
     7081 cattgatttt taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat
     7141 tttgaattca gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt
     7201 taattgttta atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg
     7261 taataggaag caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt
     7321 aagaagctga gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct
     7381 ttaataatgg gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat
     7441 agcgaagcag taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag
     7501 tcaattgcag atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca
     7561 gacaaacgaa ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag
     7621 aaagaaaggt gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga
     7681 ccgctagttt tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca
     7741 ccatatcttc ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt
     7801 tatctttcat ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt
     7861 cttttgtttc ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct
     7921 tcgttttcca agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat
     7981 gacaagtttg tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg
     8041 atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta
     8101 aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg
     8161 tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact
     8221 ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag
     8281 agcataaaga ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa
     8341 acagcttttc atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg
     8401 tttatttgaa aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg
     8461 agttactttt ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt
     8521 tatttctata aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat
     8581 aattgtgatc attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct
     8641 tgcccaagat ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc
     8701 gctattttat tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa
     8761 acgctttttg gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat
     8821 ctacgaagat agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga
     8881 agtaaagcag tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc
     8941 acaaaagaaa ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag
     9001 aatcaaaaaa agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta
     9061 ctataaagaa ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa
     9121 aatagtgatc gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat
     9181 cgtacaagaa atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat
     9241 ccgctacact acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa
     9301 ctaagcaaat tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt
     9361 gattcattga gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt
     9421 ctgagtgagg gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa
     9481 cagatgatga cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag
     9541 atagtggaaa aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt
     9601 cgttgctacc ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat
     9661 tttatcaaaa gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc
     9721 gggatgctca tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag
     9781 aacaaacaag cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt
     9841 tagaagaatc cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca
     9901 ttctccgtca aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa
     9961 agcaccatat tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag
    10021 tcctattgta cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata
    10081 tttttgtctt ccttatctta gcataaacga ttgaaaaaaa ggaaaaattt tcagaaaact
    10141 aaaatcatat cgcttcccat tttcattttt agaaaggtct gttagtataa agaaaaaata
    10201 gacaacgaaa ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa
    10261 ggaaatcaat cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg
    10321 ggagaacaag tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac
    10381 ctatgaagcg gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga
    10441 aagtgaagga aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa
    10501 tcaagaacaa aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg
    10561 agctgggagc ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa
    10621 acaagtagac gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact
    10681 aaaaaaacga gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca
    10741 tagtatcttt tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca
    10801 aggaatacaa ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca
    10861 gctgatacgt aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc
    10921 tcggttagac tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa
    10981 agaaaaatat gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg
    11041 attgctcacg gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt
    11101 actccaaaat ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa
    11161 atatgattat ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt
    11221 gaaacggatg acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag
    11281 tatctatgga ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc
    11341 taccaaaact cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc
    11401 aaatagattg atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca
    11461 aaaagaagca ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg
    11521 gctaatcaaa caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat
    11581 attataccga tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat
    11641 tccttttgta ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca
    11701 acatttctat caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca
    11761 ggcctaccag caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg
    11821 cgatcaatac ttttctccta aatatcaaaa tatagccgtt gcagattata ttttgtcgat
    11881 aagccccaat ttaaaatcga aatctgcaga aatccgagag tttgaggaag cgttgcgtaa
    11941 aacagaccag catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat
    12001 agctaaggaa atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc
    12061 tactttcaga agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa
    12121 aacccgcttg attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca
    12181 caaaagtaaa ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt
    12241 gccttataac ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc
    12301 agtaacgacg atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa
    12361 tcgtctgtat ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt
    12421 tatcaaagaa accgggagtc gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag
    12481 aagtggtcag tttcaagaaa
//
LOCUS       DACEMO010000071        12420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEMO010000071 DACEMO010000000
VERSION     DACEMO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.072"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            56..1270
                     /locus_tag="IW728_001498"
     CDS             56..1270
                     /locus_tag="IW728_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1024864.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(1980..2168)
                     /locus_tag="IW728_001499"
     CDS             complement(1980..2168)
                     /locus_tag="IW728_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024865.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            2259..2468
                     /locus_tag="IW728_001500"
     CDS             2259..2468
                     /locus_tag="IW728_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ1024866.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2580..4193
                     /locus_tag="IW728_001501"
     CDS             2580..4193
                     /locus_tag="IW728_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ1024867.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            4228..5439
                     /locus_tag="IW728_001502"
                     /pseudo
     CDS             4228..5439
                     /locus_tag="IW728_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5462..6388
                     /gene="glsA"
                     /locus_tag="IW728_001503"
     CDS             5462..6388
                     /gene="glsA"
                     /locus_tag="IW728_001503"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1024868.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6596..8056
                     /locus_tag="IW728_001504"
     CDS             6596..8056
                     /locus_tag="IW728_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024869.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            8277..10040
                     /locus_tag="IW728_001505"
     CDS             8277..10040
                     /locus_tag="IW728_001505"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1024870.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            10124..10582
                     /locus_tag="IW728_001506"
     CDS             10124..10582
                     /locus_tag="IW728_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1024871.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10691..11374
                     /locus_tag="IW728_001507"
     CDS             10691..11374
                     /locus_tag="IW728_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1024872.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            11521..12135
                     /locus_tag="IW728_001508"
     CDS             11521..12135
                     /locus_tag="IW728_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1024873.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            12151..12357
                     /locus_tag="IW728_001509"
     CDS             12151..12357
                     /locus_tag="IW728_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024874.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         4311 a         2000 c         2617 g         3492 t
ORIGIN      
        1 tgacaggcaa ttatcaatta tggtagttgc cttttttgat tggaggaatt gaagaatgaa
       61 tgataaaaac tttattgaag aactaagaca aaagcgtgag gaatacggag taacacaaac
      121 aaggcttgct gttgcctgtg gtatcagccg tgaatattac aaccgcattg aaaaaggaaa
      181 acagccattg aatgacgaat taagagaaat catagaaaaa cagattgagc gtttcaatcc
      241 gcaagaacca ctattcttat tgattgatta ctttcgtgtc cgctttccta ctacggacgc
      301 attggcgatt atccgtgatg tattacaact gaaatctaat tacatgcttt atgaagatta
      361 tggaaaatac ggatatgaaa gcaaatatgt acttggcgac atcaatatca tgtgttccat
      421 gcaggagcat ttaggtgttt tattggaact gaaaggcaaa ggctgtcggc aaatggaatg
      481 ttatctgctg gcacaggaac gctcatggta tgacttcatg ctggattgta tgacggctgg
      541 tggtgtgatg aaacggcttg atttggctat caatgataaa gcaggaatat tggatattcc
      601 aaagttaaag gaaaaataca aggctggcga atgtatgtcc tacttccgta tgcagaaaga
      661 ttacagcggt acagaaaaat gcggtagcga tttaccaaag aatacaggag aaaccttata
      721 tctcggttca acaagtagcg aattatatat gtgtgcttat cagaaaaatt atgagcagta
      781 tgtcaagaat ggcacagaaa ttgaagatac ggagattaag aaccgttttg aaatacgcat
      841 gaagaacgaa cgagcctatt atgcggttgt agatttactg acctatcggg acgctgaacg
      901 caccgctttt tctatcatca atcattatgt ccgctttgtt gacagagagg acgacaagcc
      961 aaaaagtcaa tggataacaa atgatgattg ggcatggttt gtaggggaaa acagagagcc
     1021 gatacgctta accacaaagc cagagcctta cactttacaa aaagcgttgc attggctaca
     1081 aagacaagtc gcaccaacca taaaaatggt acaggcatta gacagagaaa atcgtacaac
     1141 gatactgaaa gatatgattg agcaggcaga actaaaagac aagcataaac atttattaca
     1201 attagagaaa tcaaccatag aagaacgtat agataccgct gttccacaag agaatgacgg
     1261 tattttttaa tgtaattgaa aaatttcaga tttagtcagc aaagagctgt tttcattccc
     1321 tatgtgaagt gaaagaagaa aaataaattt tttattaaat ctgcaacaaa atggctactt
     1381 gcgtacagat atggtgcgag aaaaggaaat ctaaactttt tttgaaaata ggtcattaaa
     1441 tcggggcttg ctgtccctat atgaagtgaa agaagaaaaa agttttgaaa aaatgacgga
     1501 aattcgtgat tagtggacag tcttctgtga aaagaggaaa aacatcatca ttttgtattc
     1561 aaacaggcat tagcagttga gtgttgatgt gaaaagatga aaatactttg ttctcaactt
     1621 aacaatttaa ggtttccgtt ccctatatgg tgcaaagaaa atatttcatt ctattgttgg
     1681 cagtaacggt atggtttggg tagttagggt gtgaaaagga aaatattctt ttttcatcat
     1741 caacttagca aaatgacatt ttctatccct atatggtgca tgaaaagaag ttaaaatttt
     1801 tccatttaaa cttagcaaat ggacgtttgc tgtacagata gtagtgaaaa aggagaaaat
     1861 caaccgttga ttgccaaaat gaaccgttcc atttgtacta ataatggaaa aagttttgga
     1921 ttttggttac gtttctcctc ttttccaacg cgatatatag gaaagacgat ttttttcttt
     1981 caatcgttct ttgacaactg aataaagcta ttcaatacgt ttgacagaca gctagccgtt
     2041 gaaacaaata cgccacgacc tttcccctgc aagagcgagc gaaaacctat ttgtgttctt
     2101 gtaaaagatt tgtcctcttt tatcgccatg acctgtactg tcagataatg atactcccgt
     2161 acagccacag cttgagcatt aagagcgtcg cacgcaatgg gtacggctac ataagaacta
     2221 tgcagaggtg gaactcctgt gggctatgac aaaagccgtt gaattgctta gaaagatttt
     2281 acagaaaggg ggtatgtgtt attgataatg ctgtaaaaac aattcaaaag aaaaatggca
     2341 agtgtggcat tggcaacaga ggaaagacaa agattgtagt aaaagagcat ttttccgaaa
     2401 agggcaaaac aatggaagaa cttctaactg atgtcatgct ggaaaaagca aagcagacaa
     2461 tcgcataaaa tcggtgcagt tgtaagaaat agattgaatg acaaaaagta cccgtgttat
     2521 actttacttg caagcaggta ttgtaaacac gggttagtta taaaggagga taactgacaa
     2581 tgaaacaaca gatttacaat actgcacttt atcttaggtt aagccgagat gatgaattac
     2641 agggcgaaag ttccagcatt accacacaga gaagtatgtt gcgtttatat gcaaaagaac
     2701 atcatttgaa tgtcattgat gaatatattg atgacggctg gtcgggaaca aattttgaca
     2761 gaccgagttt tcaaagaatg attgaggata tagaggcagg aaaaatcaac tgtgttgtaa
     2821 cgaaagacct ttcccgtctt ggcagaaatt atattatgac aggacaatat acagaattgt
     2881 atttccctag tcataatgtc cgctacatag cgattgatga cggtgtagac agcgaaaaag
     2941 gcgaaagtga gattgcacca tttaagaaca tcatcaatga atgggtggca agagatacaa
     3001 gccgtaaagt caaatcagca tttaagacaa agtttgcgga gggggctcat tacggtgctt
     3061 atgctccgtt aggatataag aaacaccctg acatcaaagg aaaactgttg gttgatgatg
     3121 aaacaaaatg gattattgaa aaaatctttt ccctagccta tcaaggttac ggaagtgcaa
     3181 aaatcacaaa gcagttaaga gcagaaaaag ttccgacagc gtcatggcta aattttacaa
     3241 gatacggtac ttttgcacat atctttgagg gaaaacccga aagtaagcgt tatgagtgga
     3301 cgattgctca tgtcaaggcg atattgaaaa gtgaggttta tatcggaaac agtgttcaca
     3361 atatgcagtc tacggtatcg ttcaagagca aaaagaaagt gcgtaagccc gaaagtgaat
     3421 ggtttcgagt agaaaacact cacgagccga ttattgacaa ggaagtgttc tatcgtgtgc
     3481 aggagcagat aaaatcaaga cgcagacaga caaaggaaaa ggcaacgccg atttttgcag
     3541 ggcttgtcaa gtgtgcggat tgtggttggt ctatgagatt tggaacgaat aagacaaata
     3601 aaacgcctta cagttattat gcttgcagtt actacgggca gtttggcaaa ggtaattgtt
     3661 ctatgcacta cattcgttat gatgtgctgt atcaagccgt attggaacgc ttgcagtatt
     3721 gggctaaggc agtacagcag gacgaagaaa aggtattgaa caagatacag aaagctggca
     3781 atgcagagag aatacgggaa aagaagaaaa aggcaagtac attgaagaaa gccgagaaca
     3841 gacaaaatga gattgaccgt ttatttgcga aaatgtatga agatagagcc tgtgagaaga
     3901 taacagagcg aaattttgtg atgttgtcca gcaagtacca aaaagaacag atagaactgg
     3961 aacagcagat aacaagccta agagaagaac taagtaaaat ggaacaggat atgataggtg
     4021 ctgaaaagtg gattgagtta atcaaggaat attccgtacc aaaggaactg acagcaccat
     4081 tattaaatgc catgatagaa aaaatcctca tacatgaagc aacaacgaat gaggataacg
     4141 aaagaataca ggaaattgag atatattacc gatttattgg aagagttgag taatcaacaa
     4201 gagtaatatt tttaactaag ggaaaccggc ggtgtctata cttgggtcag tcgcacattg
     4261 ggaaaacgat tcggttttgc cgcaatcttt ttccaatggt ttgagataac agttggctac
     4321 ttgaccatgc tgtatttctt aactggtgca ttatcctatg caacgggtat tggtgctata
     4381 caaaacaata aatatctgaa attggcaatc ctattgatta ttttctgggc tattttactc
     4441 tcacagttaa aaggaaccaa atacacttca ttgattgctc ggatcggttt tgtagctggt
     4501 atcttgttgc cagcattagt cttgtttggc ttagggatcc attatgtcgc ttcaggcgct
     4561 ccactacaaa cgatactgtc tatgaaaaca ttgattcctg atttttcgaa attgcctacg
     4621 ctggttgttt ttgtttcctt tattttagca tatatgggcg tagaaacatc tgccagtcat
     4681 gcaaatgaaa tggaaaaccc taaaaaggat tatccattag caatgtttgt attggttatt
     4741 ctggcaatca ttttagatac ttttggcgga ttgaccgtcg ctactacagt acctcaacat
     4801 ggtttgtctc ttaatacagg ggtgattcaa tcgttagaat tcctattaag acacattgat
     4861 ccaaatcttg catggattgc gaaaattctt gcgattcttg tttgtttagg tgtcatcgga
     4921 gaaattgctt cttgggtaac ctctccttca aaagcacttc acgtagcagc agaagaagga
     4981 ttacttcctg aatattttgc aaaagaaaac acacacggtg ttcctgctca tttgatgatc
     5041 gcaaatggga tcgttgcaac aatctgggca gccgtcttta cattaagcgg cggggggaat
     5101 aatctttcat ttcttgcagc gatgtcattg actgttgtaa tctacctgat gttgtacttt
     5161 atgtttttcg cgggctattt gaagctggtt tttaaagatg acagtcttga aagagcctat
     5221 caggttccag gcggaaaagt tgtaaaagta atcgttgctt gtttaggtct tttgacttca
     5281 ctttttgctt tttgtatctc atttgttcca cctgctagta tcgcagctgg acagcatggg
     5341 atttatgaaa cgattctgat tgtttcttat cttatcgtct tagttattcc atttacgatc
     5401 tacgcatgcc gtcataaatt ccagcatgca gaaaaataaa aaatgaaagc tggttattca
     5461 aatggaagta aaagaattag aacaattgat tggaaaaaat ttccactatt atcaaacagg
     5521 acaagtggcg gattacattc cagcattagc tgaagtggat cccaaacaat taggtatggc
     5581 gatttatgat ttggatacaa aacaactgat tgagggagga gacgccgatg tgcgctttgc
     5641 catcgaaagc atgtcaaaag ttcccgtatt gttattagca atcaaagaca acggaataga
     5701 aaaagtcttt caaacaatca acacggaacc aaccggtttt gccttcaatt ctatcatgaa
     5761 tatggaaatc aatcatcgca aacatccaat gaatcctttt gttaatgcag gagcgattgc
     5821 aacaacctcg ttgattgccg gtaaagatgc agatgaaaaa tttgagcgaa tcttgtcatt
     5881 tatgaaagag atctgtgatg atcctaatat cacattgaat gaagaaatct atcattctga
     5941 atcaagaaca ggtgatatca atcgctctct tgcttactat atgaaaggta atcaaatgat
     6001 cgaaggagac gtaccggaga tactcgatgc ttatttcaga caatgctccc tcaatgtcac
     6061 cgctacaggc atagcaaaat tagctgctgt gctggcaaat aaaggaattg ctccttggaa
     6121 tggcaaacaa atcattactg aagaaagtgc tacaatcgtc aaatcaatta tgacaactgc
     6181 tggtttgtat gatgaatcag gagagttttc cgtccatgtt ggtgtacctg caaaaagcgg
     6241 tgtaggcggc gggttgatgg cagctgtgcc aaatcgttac ggcataggtg tcttcagccc
     6301 ggcactagat ccatttggta acagcgcagc tggtatccaa ttgttgaaag atgtagtcaa
     6361 agaactagac gctgacatct ttgaataacc attcttgaaa gaggctgggg taaaagtcca
     6421 aacggctcaa agctacctac aatgaggctg aaattgtgag cttgtttgac ttttgtccca
     6481 gtttcttttt ttattggttt ttgaaagggc ttttctaaat aaaaaggaaa caaaaatacg
     6541 atttacagat atgattaaat aaaatcataa gaaaaactgg aggcgtgtat caggaatgct
     6601 ggaaaattac atcgagcgaa atatatttag aaaagtgtat ctgtgtgaac aattatttga
     6661 attccaagaa atagacatcg aacaaacagc tatttctcta agggtgacta caccaacgat
     6721 ccttcacgat ctagaaagtc tagccgagtg tctagaatat tgtataaaag aacaagtaag
     6781 agagaagcat aagtataaac tcgtattcaa acatggtatt gctctttctg agctcacaca
     6841 gtttctgtac ggtcaatctt attttttaaa gtttttaagt tattatttta atggtatatt
     6901 cacgagtaca gaactggctg atcttgaatt tatttctttg agtaaagtat atacaatcaa
     6961 aaaaatcgta ttggattttt ttaaagaaaa tgattatttg aaaaataaag aaatcattat
     7021 cccagaattt gactcgcgga acatcttgct agctttagta cgttatgtca attgggaagg
     7081 ctatgaaaat aaaaatccgt atgtagaaga agcttgtaac gagttgattg attatgtaga
     7141 gactcatttt tttaaaagaa gatattcgga agatgaaaag tcctttatca tacgagaaat
     7201 agagatcgca atcggaagaa aggcatttcc ccttggactg aaagaagaag ataagaaaag
     7261 cgcagaaacg aagccgttat ttcaattagt ctgtcaagga ttggaagagc aaaaaggaat
     7321 tctagactta caagaagaag atgtctatta tattttttct ttgttcaaca gtagaaatta
     7381 taccaatgag aacatggagc tgttaagaaa agatttcgaa gttgtttata aaaattttgt
     7441 tctcaatgac ctatcactga atgaattgat tttacagatt attagtatcg ctgaaatcga
     7501 cgatgcagaa aattttattt tcaagcaatc ctttttacaa tttgtccgta cgttgtgggc
     7561 agatggacaa gtatttttac cggaaaggat ctatcttcta tcagaaaaag aacaacttct
     7621 gtacggaaaa attgtagaaa tattggaaga gtggaaaaag aaaaatcaaa tccaactaag
     7681 atggaatgaa aatttcattc gaaaattcgt caaaagtctt tctttgatca acagtgaaca
     7741 agcggggaga caggaaatag aaatttttgt tgtttcagaa agttcagtca aacaattttt
     7801 ttatcgaacc caatttcatt tgtatgcgga agaaggcatc gctgaaataa atcctatttt
     7861 atttcgttca ttagaagaat tgcctgatga atgtctatgt gcgaaaaaaa gagtggtttt
     7921 gtgcgatgct gctagctatc aaaatggatt agaaactgaa aagacaaaga tctatccaat
     7981 ctcattaaaa ggtgcaaaca tccatttgta tcatattgct caggaacttc acctattaaa
     8041 aaaacatatt tcttgatgag accttaatta ggagaccttc tacaaagctt tttaattgtc
     8101 tgtagaaggt ttttatttga gtcctgattc gtgcgaagat ctacttatat aacaagcttt
     8161 ctctgaaatt tttttaaaat gctattgact tagagtcaac tctaagtagt agaatagata
     8221 aagtgtaaca aatctatttt cattaaaaga agatagtacg gaggagagaa tgtattatga
     8281 gtacagtatt acatgtattg gatgcaatca atagccccgc agatgtcaaa aaattatctg
     8341 ttgaggaaat gaaacagttg gcagaagaga tgcgccaaaa aatcttgcaa aaggacagcg
     8401 atgtaggtgg gcacgttggt ccgaatctag gtgtagtaga attaacgatt gcgtttcatt
     8461 atgtatttga ttcaccaaag gataaaatcg tttgggatgt ttcccatcag tcttatggtc
     8521 ataagctttt aactggtcga aaaaatggtt ttgaaaaagg acattaccgt gaagtgagcg
     8581 gatattccaa ccaacatgaa agtaaacatg actattttac agtggggcac acatctacat
     8641 ctatcagctt agcaacaggt ttagctaaag cgagagatct gaagaaagaa tcaggaaatg
     8701 tggtagcatt tatcggtgac ggctccctaa gtggtggctt agcttttgaa ggattgaaca
     8761 atggtggtgt tttacaatcc aatctaatcg tattagtaaa cgataatgag atgtctattg
     8821 atgagaatca tggcagttta tataaaaact tagctgagct tcgtcaaacc aacggtaaaa
     8881 gcagcaataa cctatttaaa gcaatgggat ttgattatcg ttacgtggat aatggaaacg
     8941 atctggaaac ggtgatccgt ttatttgaag aagtaaaaga tattgatcat ccgattgtac
     9001 ttcacgtcca tacggaaaaa ggtcgcggtt atcagccggc aatcgataat aaaatgaaat
     9061 atcattggca tgtaccattt gatctgtcca ccggggaggc aaagcaagta acttctggag
     9121 aaacatttaa tatgatcatc atggattatt tggataagaa aatgcaaaca agtacagctc
     9181 ctattgccgc agtcaatgca gcgataccag gcgcctttgg actgaaaaca ttccaagaaa
     9241 aacatccaga tcattatttt gacgtgggga tcgctgaaca gcactctatc actttcgctt
     9301 ctgctttggc ttctcaggga gtacgtccag tcgtcttcca atcaagtact ttcttgcaac
     9361 gggcatatga tcaattatca catgatttag cgatcaacga gaatccagtg attattttag
     9421 tcggtggcgg aacgatctca gctagtgacc gaacacacca aggaacattt gacattcctt
     9481 acttatcaag tatcccgaac atcaaatatc ttgctccagc aacaaaagag gatatgatct
     9541 ctatgctaga ttgggcgttc gaacaaacag cgcagcctgt agcaatccga attcctacac
     9601 atggtgtaga acatggtaca gctatcgcaa atgattatag tattccagcc tatcaaacag
     9661 taaataatgg aggaaaagta gcaattttag ctttaggcgg ttttcttgaa ttaggaaaag
     9721 aaacagtaaa agcttttaga aaaaaaggaa tcgaagcaac gttgatcaat ccaggaacga
     9781 tcacagaaaa tgacgtagaa acattagata aattaaaaga aacacacgaa ttagtcatca
     9841 cattagaaga tggtagtttg tcaggaggat ttggcgaaaa tatcagtcgt tattatggtg
     9901 attcttcaat gaacgtatta aattttggtg cagagaaaga attcacggat agcgttcctg
     9961 tagggcagct ttatgaacgt tatcatttaa caccaaaaca aatcgttgaa gatagtctgc
    10021 gtgtcttgaa tattcaataa ttcattatgc tcaccatcca gataattgtt tatactagta
    10081 tctggatggt tttttcatta taaaaagcaa aggggtggat gttatgtatc agcaagtaga
    10141 agaatatgtc aaaaagttcc aagaaaaata tcctagttat caatcagaaa aattaggttt
    10201 cttttcattt ggggatacag aaaaaatggc agacgagttg agcgaactag ttgtaaaaaa
    10261 gataaagact ggtacatcaa gtggttacga tctgtataaa caagagaatg agcctttacc
    10321 aaaagttggt caattagacg tggtcctgaa tggaaaaaat gaaccagttt gtatcatcaa
    10381 aaatataaat gtgaccatct tgccttttag tgaagtcacg aaagagcatg cttggaaaga
    10441 aggagaaggg gatcggacat tagcttattg gcggaaagtc catgctgagt tttttaaaca
    10501 tgcttataaa gaagcgttga acatagaatt tacagaagag aagctggtcg tatacgaaga
    10561 atttgaagta attttctttt agtaagaaag gtctgctgaa agaaatagct cattattgtc
    10621 tcctgaatag aaaagggtta catttttata tgaagctgta tttttatata acattactgg
    10681 aggagatatt gtggatacac tggaacaaat caaaggcgga gtcgtcgttt cttgtcaagc
    10741 tttggaaaac gagccgctac acagttcttt tattatgggg agaatggctt tggctgcaaa
    10801 agaaggcgga gcagttggta ttcgagcaaa tacgacagaa gatatcaatg aaatcaagcg
    10861 aatcgttgat ttaccggtaa tagggattat caaaagagat tacgatgggt cagaagtgtt
    10921 tattactgcg acagaaaaag aagtggaaga acttttggag acgcaagcag aaatcattgc
    10981 actggatgca acttctcgta aacgtccgac agaaaaaaca actgctgaac ttgtccaaat
    11041 gattcacgaa cacggtcggt tagctatggc agatatttcg acttttgagg aagcgcttca
    11101 agcgcaagaa gatggttttg atcttttgtc tacgacttta gcaggctata cagaatattc
    11161 acgaaagacg cagacacctg attttgattt attgaatcaa atcatagaac aagtcgatat
    11221 cccagtaatt atggaaggac atactgactc tcctgaacaa gtagagaaag catatgaatt
    11281 aggtgctttt tcagtcgtag ttggatctat tatcacccgt ccgcaattga tcactaaaag
    11341 atatgtcgaa gccgcaagta aagcaaataa ctaaaaaaca cgcacggcac agattcttga
    11401 caaaaaagaa aaattgatag atagtttaaa gcagattcta agaagatgaa aaaagcatta
    11461 cttttcattt tgtcttcaag atctgctttt taacgaattt tatagaaaga aggcgaaacc
    11521 atgtttgaac ggagtccttc agaattgaca gaacgtgaaa attacaagtt gctgattggt
    11581 tcagtcatcc ctagaccagt agcagtagta acgactaaat cagcaaaaga tatagtgaat
    11641 atcgcaccct tcagttactt caatatcgtc agttcgaatc gacccatctt atccttggcg
    11701 atccagagaa aacaagatgc agtaaaagac acagcaaaga atattcttga gacaagagaa
    11761 gctgttatcc atatccttga tgaggacaac gtagaagaag taaataaaac agcagccaat
    11821 ctgcctaatg aggaaagcga actgtacctg actgacttga ctttaactga ttcagtaatc
    11881 atttcagtac cagcattgac agaagcaaaa gttcgttttg aaacatcctt atatgaacat
    11941 gtcgagatac ggaataaaaa taaagtaaca gctgatttat ttttgctaga agtacagaaa
    12001 tatcatttaa atgaaacagt ctatgatgat aagacaggaa ggatcgatcc aagaaaattg
    12061 aatgctgtaa gccgtttggc aggaaatgat tatgcagcaa taggagaaat tttcacgatt
    12121 gcacgtcccg aataaaaagg agcactagtt atgttaaaaa taagctataa accatcgaca
    12181 gattcaaaag aaatgaaaaa agaatatgaa acagtgaatg attttctgca agggcagtac
    12241 ttagaagttc cgccattgca agaccatttt gtcgttacta ctgttacttt agatggtaag
    12301 gaaatagaaa tgccagatca aacgattagc ggattgttta attactttaa caaataaata
    12361 tttattccga aaagtgaaaa aacatataaa aaaacacata aaactaaaaa attatcaaag
//
LOCUS       DACEMO010000072        12339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEMO010000072 DACEMO010000000
VERSION     DACEMO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.073"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..809)
                     /locus_tag="IW728_001510"
     CDS             complement(<1..809)
                     /locus_tag="IW728_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1024875.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEEN"
     gene            1016..2047
                     /locus_tag="IW728_001511"
     CDS             1016..2047
                     /locus_tag="IW728_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1024876.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            2136..2441
                     /locus_tag="IW728_001512"
     CDS             2136..2441
                     /locus_tag="IW728_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1024877.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            2620..4635
                     /locus_tag="IW728_001513"
     CDS             2620..4635
                     /locus_tag="IW728_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1024878.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            4637..4921
                     /locus_tag="IW728_001514"
     CDS             4637..4921
                     /locus_tag="IW728_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1024879.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            4947..6320
                     /locus_tag="IW728_001515"
     CDS             4947..6320
                     /locus_tag="IW728_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1024880.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(6403..8418)
                     /locus_tag="IW728_001516"
     CDS             complement(6403..8418)
                     /locus_tag="IW728_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1024881.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(8440..9654)
                     /locus_tag="IW728_001517"
     CDS             complement(8440..9654)
                     /locus_tag="IW728_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1024882.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            9875..10819
                     /locus_tag="IW728_001518"
     CDS             9875..10819
                     /locus_tag="IW728_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1024883.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            11115..12059
                     /locus_tag="IW728_001519"
     CDS             11115..12059
                     /locus_tag="IW728_001519"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1024884.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         4037 a         2221 c         2331 g         3750 t
ORIGIN      
        1 atattctctt caaaattttg agaatatatc actgagaata acgtatttaa tacgtaatcg
       61 aaggcaattt gagaggagaa agtcgctact ttagcaaaat catattcctc ctgcgatata
      121 acaagacaca tatcagcctg tttggccatt tctgaatgct gatttccggt aatcagcaag
      181 gtaggggcct ctacatgctt caaataggtc attattcgtt cataatgatg gatgcgaccg
      241 ctgtagctaa taaaaatggc gcaatcctcg ctagtcaaat tggctgcatt ccaagaagaa
      301 tcactgtatt cttcagccat gatcaaaaac ttattcaact tgaccatttt attctggaat
      361 ttccttgccg tgatttgaga atcacctttt gcaaaaagaa agattcgctg tgccttgttc
      421 aatactttac cgatttgatc taaatcttca tgttcaatct gcaattgtgc tttttttatt
      481 gactggatcg ataaatccgc cattttcttt gcaatttgct gagtgctatc ttcacttgaa
      541 aaaggaaaat taggatcaat cgtttgattg aactgagcaa tcaattgatg aacttcccgc
      601 cccagctcga aacggaattc tttaaatccg ctgaatccca tttttttgca taaacgaatg
      661 atagctgaat gggaagtata cgtagctttt gctaattctt caatcgtcat gtagactgca
      721 gcttcgatat tcctacggat gtaatcgaca atccgtttct ctgtcgtggt aaacaactct
      781 tgttttgtta gtttttcttc aataagcatg atctgacctc caaattctat cttactgata
      841 aacacttggt tccgctacta tctttttgat ttttatcctt actttgaaca ttaatttcac
      901 caaaccgaat gataacaagt gtttttctga acttttttac cagaatagct attataaata
      961 ctttctgcgc ttttctagga tatactgaaa acatcaataa aaggaggcac cactcatgct
     1021 tacaatcgct tatcttggca atggaaagag tacaaatcgc tatcatttgc ctttttcaag
     1081 aaaacttacg aacaaaatca atatcaaaaa aattttttcc ccatctgaac cagtgtggaa
     1141 aacattcgaa gacatcgaat atactgatca gctgtctgat ctttgggatg atcctgagat
     1201 ccaattagtt gtcatcacta cacctagtca atttcattat gaatatggaa aaatggcact
     1261 cgaacatggt aagaatgtac tagtagagaa accttttgca gaaacgtcaa aggaagcaga
     1321 agagttattt gcattagcaa aagaaaaagg tctatttatc caatgttacc aaaacagacg
     1381 gttcgattct gacttcttga cagtccaaaa agtaatcgaa agcggaaaat taggggatat
     1441 tttagaagta gaaatgcatt atgattattt cagaccggaa gtaccagaaa atacgcatga
     1501 attttctact gcgaacagct atttatatgg acatgcttgt catacagtag atcaagttct
     1561 ttcttatttc ggcgaaccag atgatatcag ctatgatgta cgtcaacttt tagggaaagg
     1621 acgaatgaat gattacttcg atctggattt tttctatgga aatatgaaaa tttcgataaa
     1681 atccagttat ttccgcatca aagaacgtcc aagtttcgtc gtttacggaa aaaaagggat
     1741 gttcgtgaaa caaacaaaag atcgacaaga agaacatctg aaaatgtttt acatgcctga
     1801 tcatgaagac tttggtatcg atttgcccga acattatggt gtattgacct atatagatga
     1861 acaagggagt tatcatgaag aaaaagtaat ttccgaggtt ggtgattaca gccgtgtcta
     1921 cgaagggatt tacgagacat tagttaatgg cgcagaaaaa gtcatcaaag atgaagaaac
     1981 aatcttacaa atgaaaattt tggaagaagg aatcaaaagc attcaagaat cagaaggaaa
     2041 aatatgacat atttgtaaga atcgggtttg ttttttgacg cataaactgt cttattgtac
     2101 gatgaaaaag tatagaacta ctaggaggaa ccaacatgac aaattatgac ggtgaaaaac
     2161 acaaacgatt aagaggttca atggccccta aaacagaaga agaagtggct ttagcaggaa
     2221 ctaattctgg tgaaacagaa gacaaagaag aatcattagc tggcacaaac tctggtgaaa
     2281 cagaaaataa agaaacagct ttatctggtt caaattctgt agaagaggaa acaaagccta
     2341 atgagcccaa tcactttgaa aaagatatcg aaaaagcggt tgaagatgtc aaagaaaaat
     2401 ttgaagaact gacctcaaaa aataaacatg atcatcattg atcggacgaa atcaattcat
     2461 aaaagcagga gacatttggt cacctgcttt ttttctgtat ccattcgata gaaatcgttc
     2521 ggtacagtcg taaaaaaaat ccaactgtaa gttgtaccaa acacccctcg atttaaaagc
     2581 gctaccatac tagaatataa gtaggaggtg aacagcgctg tgaactacaa aattagttat
     2641 ctcctgaaaa caccaagaga aatcagtcta gaaaaagcag cagagatcat cgaagtaaat
     2701 acaagcgagg cgatcaaaca attaactcct tttgccgaat atgtggatga tacccgtatc
     2761 cgtataaaga aattccgagc aatcgactgg gcagaaaaat ttttggatat tactcgattt
     2821 gaagctgtgt ataccgaaca agagcgaaga gatcttcttt atttgatggt ttattcgact
     2881 gcaacgtctg tttctgtttt tcattttcaa gattttcttc aggtttctaa agggacagtc
     2941 ctgtcagata tcaaaaagat aagaaatgaa ttagaagtgg aagattgtca gctgtcgtat
     3001 actaggaaaa ctggttttag gctgctaggg tcagaagcaa tattaagaag agaagcaaaa
     3061 aatcgcactg ctcgtttgat gcagtcactt gctggaagat ttgggctcac gtatttaacc
     3121 gcaacccttt ccattgatta ttacgccaaa gttagagatt tgctagataa aacgatttca
     3181 ctatcagatc ttcgattcgc accaaatcga atggatgaat tagcttatta tttgagtttg
     3241 acaaccattc gaattggaaa aacatccatg gaactggtaa aagatgctga actgttggct
     3301 tctcttgcag tcactgacat tagtcgacaa gtattgactg gccttggcta ttccgaacat
     3361 caaaattcgg agattatatt tttctctctt tgcttagcat cgatcgttga aggagatgtc
     3421 caggagcctg cacttgattt tttgctggaa tgttcttcag aagtcatcca tcgcatggaa
     3481 ttcctgatgg ccgtagaatt tgcttcgttt cgtgaattat tgatgaatgt cttttatcac
     3541 ttggtccctg cctattttcg tatttcctat tctttttatt tacctaatgt gatgatcgat
     3601 caaattaagc atcaatatgc ttccatctac gaaatgacaa gaaaagcatt acttccgtta
     3661 gaaagacgaa tagggaagag catccctgaa gaagagattg gttttttcac tatactattt
     3721 ggcggagaga tcagaaaagc ggacgcagaa gaaagaaata gaaaaataag agccgtgatc
     3781 gtctgtccaa gtggcattag ctcttcattg atcttaaaaa gtgaacttca gcaattattc
     3841 ccgatgatct tttttacaga gactaattca gctgaccgct tacacgaggt tgacgaaact
     3901 tcttacgata ttattttttc tactgtacct ctcacactta ctgataagaa aaagtctctc
     3961 tatgttctgc atccaatcat gactgacttg gaaaaaaatc agctgatcaa tcaagtacaa
     4021 agagattggc tgataccagg attttcaatg ccttctgccc aagaattgtt gtcggcgcta
     4081 ctgccttata tcgaattaaa agaaggcgta gacgaagaaa agctctatca agtattaaat
     4141 cgtaaaatga acaaactttt agaaaagaag gaggatcaac gtcctatgct atctgaatta
     4201 ttaacagaag aaatgatcca attatcggat caaccaaaaa actggcaaga agcaatcaca
     4261 tttgccgcac agccgctatt atcagaaaat aaaatagaag aaagttatat caaagctatg
     4321 atcgagcgcg tggagcagta tggtgcgttt atccatattg gcgatcacat cgcattacct
     4381 catgctcgtc cagaagatgg cgtaaacgaa gtagggatgt ctttgctgaa acttcaacaa
     4441 cctgtggatc tgcttgataa tccagcccat cccattagtt tgttcatctg tttagctgct
     4501 atcgataatg aggcacatct tcgggctttg gcaaatttaa caaagctact ttctaataaa
     4561 caaaatcttc aagatttatt acacgcatca acaaaagaag aaatattagc gatcataaga
     4621 aaaggagacg aatgatatga aaatttttgc agtatgtcaa agtggattag gaacaagttt
     4681 tatggtagag atgaacatca aacaagcact tgcagatctt ggagcagatg tggacgagtt
     4741 tgaagtaagt catacggatg ttggaagtgt atctagcgat atggctgatt atttcttcat
     4801 tgagcattct ttgaaagata cgctaacttc tatacctgat gaaaagctcg tacctttgca
     4861 atcgatcatt gacagcgaag aagtaaaaga aaaagtaacg aagatcttgt caaaagaata
     4921 aaaatgattc aagggaggaa ctatcaatgg ataaattgtt gaatttgttt gtgtcaatcg
     4981 tcagtcagcc ctccatcctc gtttctttga ttgcactgct tggtctcgtt cttcagaaga
     5041 agaaattttc agaagtgatc caaggaacta tcaaaacatt tgtcggattc ctagtactga
     5101 tcggcggtgc tggtttaatc agtaattcac taaccccttt tgcttcgatg ttccaaacag
     5161 ctctgcatac tcaaggagta gttcctagca atgaagcagt agtggcgatt gctttgcaac
     5221 aatacggtac acctactgct ttgatcatgc tagtagggat gatcgtgaat attttacttg
     5281 ctagattcac aagatataaa ttcatttttc ttacaggtca agcaatgctt tatgtttctt
     5341 gtctaacagc agtcatttta gtaagcgcgg ggtttactgc aaatttgtgg atgatcttat
     5401 taggcggggt cttcgaagga acactattaa ctattacgcc tgctctttgt caaccgttta
     5461 tgaagaacat cactggaaac aatcaagttg cgttagggca taccggaaat atcggttatg
     5521 cttctagtgg atggatcgga aagttttttg gaaacaaaga ccgttctaca gaggacataa
     5581 agattcctaa aagtttcagc tttttgcgag attctactgt atcgatcatg ctcttgatgt
     5641 cgatcgtata tgtcgcactt gctttgttag ctggtcctgg atttgttgaa agagaactaa
     5701 gcaatggaga caacgctgta atctttgcac tgatccaagc tggaacattc acagctgggt
     5761 ttgttatcgt ccttcaaggg gttcgaatgg tgttggcaga aatcgttcca gcattccaag
     5821 ggattgctaa aaaactcgta cccgattcta aaccagcatt agatgttcct atcgtttttc
     5881 catatgcacc taatgctgta ttaattggct tttttgttag cttctttgta ggaacactat
     5941 ccatgtttgc tatgataggc ttgcaaacta cagttatcat tcctggggta gtaggacatt
     6001 tcttctgcgg agctgcttct ggtgtcttcg gcaactcgac tggcggtaga agaggggcgg
     6061 tattaggttc tgctttcaat agcttattga ttagttggct gccattattt atccttccag
     6121 tattaggaga tcttaaaatc gcttcttcta catttgccga tactgattat ctagtaccag
     6181 gacttgtact tgggaaacta ggagaatacg gtcaaatagc cttgacagtt ggaatcatcg
     6241 ctttcttttt acttgtcgta ttatctagct ttctttttgc cggaaaaaga acaaacattg
     6301 tttctaaaga acagaattaa gcaaacttca aaataaaaat tgcttggaca atatcagaag
     6361 aagatttaaa gtcttctgat atgtcgcaag cagttttttg ttttatgcat ttatttttga
     6421 atagcttgtt aagacaagtg gctgttttgc tagaagagga taacttttac cattcatttt
     6481 cactgttccg cctgttctat cagaagtaat cacgatctta tcttttgttt tttcaagcga
     6541 aatctttcca tctttgatag gtagcgtaca attcagtgtt tcaaatgaat ctagattagg
     6601 ttgtatcata aattcttcat aacctggctt tgttggatac aaaccgataa aatagcgtcc
     6661 taaaagatag attgggcttg ctccccatgc atggcataag ctttttccat aaggatctcc
     6721 atacatactg tacttttcat ttcctgtttg agtagggtcg tattcttccc agaaagttac
     6781 cgcatcatga gataacatcc ctccccaata atcaagaatc gtttggtaga cgatattcgt
     6841 ctgtccgagt ttgcataaag catcttgttc atagaatttg aaatatggag tagtaatctg
     6901 tgtgatagtg tcattcaata agacattctt taagatcgat tgctgttggt ctttgttagc
     6961 aaaatcgaat aaaacagcga aaatattcgc atgacgcgtc acatttcttc tcccagattc
     7021 atagcaatcg ataaaagcgc caagttcttc atcccaaaag tagtcaaata ggttttgttt
     7081 caatagttga tatttttctt gatagagatg agtatgataa ttttcgttct ctccatatgt
     7141 ttcagctaag atatctccgc aagtgatcat ggaatgatag gctttcagta ataagacttg
     7201 ttctgcagca agcgttcctt ctttatccat ttcagaccaa tcaataaata tccaatcttt
     7261 tgctctccca taaataaacc ctaattcatt cgtttgctcc atcaagtatc tcattaatga
     7321 ttgcatctta gggtagacga tgcgtaaaaa ttcatagtct ttcgtcatca tatagtggtt
     7381 ttcgatacca atcaaccaca agatcgaata gtcaacgata gtattcatat gctgcttgat
     7441 ttcattttgt cctcttaatg ctaatatcgt tcgtttattg atttcctcgt caaaaaaaag
     7501 atattgattg atcatgaatg attgatacgc atctccagac caaatccagc gatctcgttt
     7561 gataccgtcg ataaaaaaca aatcactaca taacttgaag gcttcttctg atactgacca
     7621 gatctgattc agtaaagaag aatcgcttga aaaagcagct tgtttcgaaa gagataaaaa
     7681 ttcatgaaaa acttgaagtt gaaccattcc agtagaaacc tctggtataa acaaatagcg
     7741 aaaagcgcgt ttacgagctt tcatttttct tgtcattcct atttgcttat agtagcaata
     7801 atcgatatcc atagcttcta catcggactc tccataacat agagtgatag attcgatatg
     7861 gtcatccaga gatattagtt ctacacagcc attcactgct cgcccaaaat cgaataagat
     7921 tcctccgcca atctccgttt cagtgactgg atggacgcat tctttttgat aatagatttg
     7981 atttggatct tttgcgatat cgacataaag cggactccag ccagctggat attcttcaat
     8041 aaaattactt gctgtccagc caatatctga ttttacttcg tctccctcga taaacatcgc
     8101 tggtagccct gatgtatgtc ctacaaagat acgaattttt gctttaccag ctggacaagt
     8161 cagccatttt ccacatggat gtttttgtcc attaatttct acatacccaa ctccttggat
     8221 cgtcacttta aacatggaag gttgatcaag aaagtaatat ctcttaaact tgacgttccg
     8281 atggcaatca tccatgtacc agtacgctgg ccagtcaaaa cctcgttctt cccgttggaa
     8341 attttgtagc agtccatggc ggatctcaaa gtctccagga taccaaagcc atctatcttt
     8401 atgtttttca tttgtcattt tacacgacct ccatcaaatt tattttgttg tcaattgttc
     8461 ttttatttct tcatctactc ttatcactgt ctttctattc gtcctgtgta ctactagaag
     8521 gctaataagc gcgctaatca acagcaagat actgccattg acgaaaacag caagtgtttt
     8581 actcccaaga gaagcaaaag agagaaagag caaaccatat ggtgcaatga aagtacctaa
     8641 attagcgcca accaataaga ttgaagtatt gaaactctcc gcgtacttgg gcgcttggat
     8701 acttacttgg ttgaataagt aaggaatgaa taacgaaaaa gaaattcctg ttaggatcgc
     8761 accaaaccca atcacataca ggtttatcac agtggcaatc aatacaaagc cgattcccat
     8821 cagtagtaaa gaaagtgtaa gcatatagcg atgtgctttt gtctgaagcc agccaaaaca
     8881 aactccggca gccatacttg ccaattgcat gattgttaag atattcgctg cattagtttc
     8941 gtttcctaga gaataagtcg tcaggactgt ggctaattta atcgatacag cattatatac
     9001 cacgatgacc agcaagagaa aaataccata actaatcatt ttaggattcg ttttgattac
     9061 ttgtcttgtt ggggctgtgc catgcgattg aagttcagtt gtttcaggtg taggaacaaa
     9121 caaaacaaac aagacaagaa taggtaagct gatggcgtag actaaaaagg tatattgcca
     9181 accgaactgt actagctgtc ctgcaacaaa tgtcattaaa gctgcaccaa atccttgaaa
     9241 tgcactttga aaaccaatca tagaggctcg ttcatctccg tgataaaagg cagcaatcat
     9301 gctgactgcc aaagaattga tcaatccaaa gccaatacct agtccagctc ttgaagctaa
     9361 gacaatactg taactttgtg agaagagggg gaggatacca caaatcgatg caatcactaa
     9421 tccgatgatc acagtttgtt tttgtccgat tttcctagca ataaaactac taatcaaaac
     9481 aaaaatagtg accataaaag agggagcagt cgttaataat tcaacagcag ataaagaacg
     9541 cgtggcataa gtagctgaca tactgggaat tgcagacgtg actgcaccag cagaagtcaa
     9601 aataagagag actgacagca gagaaaattt catcatcttt ttattcataa gcatgttcct
     9661 ttcatttttg taagcgctta tcaaaaatat agtatgaagt ggttattctc tcaatagcgg
     9721 aatcacagga agttcatgtc ccaaccatag aaagtcttta tgaaaccaaa aatagctttg
     9781 catataatag cagtacagcg tgaactaata agcgaaagaa accgcttaca aaaaggaggg
     9841 agtcaattgg aactagctga attagacatt ttttttgcgt aacgaagaat caatcgaaac
     9901 tctgcagcgt gaacaaggaa tgaatatcaa tgatttaaac ttagcttacc atcttcagac
     9961 accgccaaaa atccaccgag gggatctatt tcaacaggga aatattcata tcagtaaaca
    10021 ccgtcgatat gcggaagttc ccgagcatac acatgatttt gttgaaatca attatatgta
    10081 tagcggtaca tgtttacaaa ttattaatgg agaaaggata gagatggaag aaagtgacgt
    10141 ggtgatcatt gataaagaag ctactcaaag aatcgaatac acagggcaag aggatatttt
    10201 aatcaacatc ttattaaaag aagaaacctt atctacagag atattgaatc atttggcaag
    10261 cacaaataat ctactttctt cttttttgat aaatgcatca aaagaaaaag gggaacatac
    10321 acaattcatt cattttcgcg gcggaaaaac aagtcgtttg acgcatttga ttgaaaatat
    10381 gctgttgatt tattttgata catacagtca taagatccgt tcgttaaatc tctatacatc
    10441 actattactg atcgaattga ctcatttatt agaacaacaa agcttttcat cagcagatgg
    10501 atgcgcagaa aatgagatgc ttagagtatt agattatatc gataagcatt ttacttcttg
    10561 tacgttaaaa gaaacagcag ctcattttgg ctacaatccg gtctatctta gtaataaatt
    10621 gaaaatgtat tttgatgctt cctttcaaga actggttcta aagaaaaaat tcgactttgc
    10681 gttggaactg attcaagaaa cgagtttttc gttggaagaa atcgctaatc gtatcggata
    10741 taaacaaacg acttctttat tcaaattatt caagaagtat tcaacatgta ctcccaatga
    10801 atggagaaat aaacattaag aagaaaatct ttcactaaag catatgaagg aaaattgttt
    10861 taggtgcgat tgctgtctta ttatatcgtt gcagcgaatt tttttggatt tattttcagt
    10921 tctaaacttt tggttgtatt atcaggggat ataggtgtaa acatttgtca tttatctgtt
    10981 atgcgtattc aggaattcgt ttccatcaaa aattataata acaactatta taattaacat
    11041 gttgataaaa tgaataaaca agactatact tttcatgaaa gtaacaaata gttatgaaaa
    11101 ggagctgttt attcatgaaa aaaacaagtc gtaaagtagt aatcgtcggt acaggttttg
    11161 ttggaacaag tatcgcttat gcaatgatca accaaggaat ctctaatgaa ttagtattga
    11221 tcgatgtgaa tcaagaaaaa gcagaagggg aagcattgga ccttttagat ggtatggctt
    11281 ggggcgatga aaatgtggct gtatggtcag gtggctatga agaatgtaaa gatgctgata
    11341 tcgttgttat cacagctggt atcaaccaaa aacctggtca atctcgttta gatttagtta
    11401 aaacaaatgc gtcaatcatg cgccaaatcg taaaagaaat catgggatca ggattcgacg
    11461 gtattatcgt tgttgcttca aacccagtag atattttgac atatatcgct tggaacgaat
    11521 ctggtctgcc aacatcacgt gttatcggta ctggaacaac attggataca actcgtttcc
    11581 gtaaagaaat cgcattgaaa ttaaaagttg acccacgcag cgtccacggc tatatcttag
    11641 gtgaacatgg tgattcagaa gttgctgcat ggtcacatac aactgtcggt ggtaaaccag
    11701 tgtttgaaat tgtagaaaaa gaccatcgta ttgcccaaga tgaattagat gtgattgctg
    11761 ataaagtccg taatgctgct tatgagatca ttgatcgtaa aaaagcaact tactatggta
    11821 ttggtatgag tactgcacgt atcgtaaaag ctatcttgaa taacgaacaa gctgtattgc
    11881 ctgtatctgc ttatttaact ggtgaatacg acgaaaaaga tatctttaca ggtgtgccat
    11941 caatcgttga cgaaaatggt gttcgtgaag ttgttgaatt atcaatcaac gaagaagaaa
    12001 aagcaatgtt cagcaaatca acaagtgctt taagagaagt attgaacact gtattataag
    12061 aaacaatgaa tatgaactaa aaatcctaaa actgcttaac agctgaaata gctgttaagc
    12121 agttttttat tgatatctaa taggtaagca aataaacaga atggttaaaa ttaatcggtc
    12181 cgaaaatgta tccgtacata tgaacatatg aaatttaatc tgttgattac aataagcgaa
    12241 tcgttatcaa tgtttatctg tggttaatta aaaaatcctt ccgcaacggc aaatacaatg
    12301 attcatatct aattcgttca tatttgatta actgaataa
//
LOCUS       DACEMO010000073        12074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEMO010000073 DACEMO010000000
VERSION     DACEMO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.075"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(133..1440)
                     /locus_tag="IW728_001520"
     CDS             complement(133..1440)
                     /locus_tag="IW728_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1024885.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1443..2024)
                     /locus_tag="IW728_001521"
     CDS             complement(1443..2024)
                     /locus_tag="IW728_001521"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1024886.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2140..2237)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2596..3888)
                     /locus_tag="IW728_001522"
     CDS             complement(2596..3888)
                     /locus_tag="IW728_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1024887.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(3885..4508)
                     /locus_tag="IW728_001523"
     CDS             complement(3885..4508)
                     /locus_tag="IW728_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024888.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4511..5347)
                     /locus_tag="IW728_001524"
     CDS             complement(4511..5347)
                     /locus_tag="IW728_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1024889.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5364..6251)
                     /locus_tag="IW728_001525"
     CDS             complement(5364..6251)
                     /locus_tag="IW728_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1024890.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6267..7550)
                     /locus_tag="IW728_001526"
     CDS             complement(6267..7550)
                     /locus_tag="IW728_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1024891.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(8233..8916)
                     /locus_tag="IW728_001527"
     CDS             complement(8233..8916)
                     /locus_tag="IW728_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1024892.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(8934..9551)
                     /locus_tag="IW728_001528"
     CDS             complement(8934..9551)
                     /locus_tag="IW728_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1024893.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9561..10385)
                     /locus_tag="IW728_001529"
     CDS             complement(9561..10385)
                     /locus_tag="IW728_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1024894.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10378..10938)
                     /locus_tag="IW728_001530"
     CDS             complement(10378..10938)
                     /locus_tag="IW728_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024895.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11112..12068
                     /locus_tag="IW728_001531"
     CDS             11112..12068
                     /locus_tag="IW728_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1024896.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         3630 a         2653 c         2047 g         3744 t
ORIGIN      
        1 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
       61 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
      121 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
      181 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
      241 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
      301 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
      361 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
      421 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
      481 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
      541 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
      601 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
      661 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
      721 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
      781 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
      841 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
      901 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
      961 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     1021 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     1081 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     1141 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     1201 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     1261 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     1321 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     1381 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     1441 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     1501 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     1561 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     1621 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     1681 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     1741 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     1801 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     1861 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     1921 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     1981 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     2041 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     2101 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     2161 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     2221 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     2281 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     2341 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     2401 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     2461 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     2521 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     2581 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     2641 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     2701 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     2761 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     2821 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     2881 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     2941 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     3001 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     3061 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     3121 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     3181 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     3241 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     3301 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     3361 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     3421 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     3481 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     3541 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     3601 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     3661 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     3721 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     3781 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     3841 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     3901 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     3961 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     4021 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     4081 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     4141 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     4201 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     4261 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     4321 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     4381 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     4441 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     4501 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     4561 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     4621 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     4681 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     4741 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     4801 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     4861 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     4921 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     4981 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     5041 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     5101 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     5161 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     5221 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     5281 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     5341 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     5401 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     5461 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     5521 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     5581 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     5641 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     5701 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     5761 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     5821 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     5881 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     5941 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     6001 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     6061 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     6121 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     6181 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     6241 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     6301 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     6361 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     6421 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     6481 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     6541 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     6601 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     6661 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     6721 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
     6781 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
     6841 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
     6901 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
     6961 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
     7021 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
     7081 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
     7141 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
     7201 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
     7261 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
     7321 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
     7381 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
     7441 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
     7501 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
     7561 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
     7621 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
     7681 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
     7741 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
     7801 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
     7861 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
     7921 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
     7981 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
     8041 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
     8101 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
     8161 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
     8221 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
     8281 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
     8341 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
     8401 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
     8461 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
     8521 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
     8581 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
     8641 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
     8701 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
     8761 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
     8821 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
     8881 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
     8941 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
     9001 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
     9061 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
     9121 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
     9181 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
     9241 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
     9301 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
     9361 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
     9421 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
     9481 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
     9541 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
     9601 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
     9661 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
     9721 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
     9781 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
     9841 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
     9901 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
     9961 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    10021 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    10081 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    10141 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    10201 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    10261 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    10321 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    10381 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    10441 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    10501 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    10561 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    10621 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    10681 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    10741 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    10801 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    10861 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    10921 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    10981 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    11041 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    11101 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    11161 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    11221 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    11281 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    11341 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    11401 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    11461 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    11521 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    11581 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    11641 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    11701 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    11761 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    11821 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    11881 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    11941 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    12001 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    12061 atatgtaatt ttaa
//
LOCUS       DACEMO010000074        12064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEMO010000074 DACEMO010000000
VERSION     DACEMO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.076"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(147..488)
                     /locus_tag="IW728_001532"
     CDS             complement(147..488)
                     /locus_tag="IW728_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1024897.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(556..2727)
                     /locus_tag="IW728_001533"
     CDS             complement(556..2727)
                     /locus_tag="IW728_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1024898.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            2938..3795
                     /locus_tag="IW728_001534"
     CDS             2938..3795
                     /locus_tag="IW728_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1024899.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(3866..4723)
                     /locus_tag="IW728_001535"
     CDS             complement(3866..4723)
                     /locus_tag="IW728_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1024900.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(4708..7410)
                     /locus_tag="IW728_001536"
     CDS             complement(4708..7410)
                     /locus_tag="IW728_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1024901.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(7423..8712)
                     /locus_tag="IW728_001537"
     CDS             complement(7423..8712)
                     /locus_tag="IW728_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1024902.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            8862..9908
                     /locus_tag="IW728_001538"
     CDS             8862..9908
                     /locus_tag="IW728_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1024903.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            9910..12063
                     /locus_tag="IW728_001539"
     CDS             9910..12063
                     /locus_tag="IW728_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1024904.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         3317 a         2552 c         2200 g         3995 t
ORIGIN      
        1 tccttgtaac tcagagttaa acggtaggct cacgacctct tggtaaatgg tgttcaagag
       61 acagcctatc ggcaataaga agcaattttg tcatcgctca aggccgaacg tctctttcac
      121 accctctgtt gtttcataaa atagttttat tcgctgtaaa ggtcgcgaac tggtgtcatg
      181 atgatacggt tcaagtcatt gatgattgta ctgaagcctt gctctttttg catcaagtca
      241 ttgattactt cttctgattg gattttcaca gccatttctt gtgctttcat tgcatcttcg
      301 tcgctgaact cttgtccact catttgtttt tcttgtaatt cgatttgcat cgcttggaag
      361 tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt tctcgtacgc ttctttcaac
      421 gctttgaatt ctggtaattc acggatctca cgttcgattt gattcgcgct atcgtagatg
      481 tttgacatgt tatgccctct tttcttttag tagttactct ctattgtaac atggggttat
      541 tcgttttcaa aggggctact ctccttgata tttccgccag aggtctttag ccgcttcacc
      601 aattccctga acgccttctt tcaccatatc gccgaagttt ttcagccctt cttttgcttg
      661 ttcaccatat tgattcaagt tatcttgcca atttccagac tctccattag aatttcctgt
      721 gtttccaact tgatctgcag ctaccacttg tcctcctgtg gcataggcat ctgctacact
      781 aaatcctgtt tttgaagtat acggaaggat gccttctgcc gctgatttga agactttccc
      841 gacaacattt ccacttgtcc cttccaaata gtgcagcttg ctggattctt cgtaacccat
      901 ccatgtcgaa atcactacgt ctggcgtgta accgatcatc cattggtcat ttgcttttgt
      961 ggcatcgaaa ttagtttctg tggttcctgt tttccctgct atcgtataac ctgcaggatt
     1021 cgcagcagcc gctgttccat ttgaaaatgt accaagaagc atgctggtca tttcatcggc
     1081 tgtttccttt gatatgacct gttttggttt tttgtttgtg ttatcgacga tcaccgctcc
     1141 agtcgaatca acgattttcg tgataagatg cggcgtatac atctgaccat cattggcaaa
     1201 tgcactgtaa gcacccgcca tgatcaatgg agatactcct ttttccagtc caccaagggc
     1261 taagccataa tacttatcag attttgctaa aggcaggccg aattcttctg ctttatcata
     1321 tcctttttgt aaaccgatct catgcaaaag ccatacagta ggcaaattca aactttgtgc
     1381 aacagcttga tacatcggta cttctccttg gtatgtcccg tcaaaattat gcgcatcgta
     1441 ataactttgc ggttcgtctt tcaagatact ggaaggcttg tatccagctt ccagtgctgg
     1501 tgtatatacg cttaatggtt tgatcgttga tcctggtgaa cgacgcatct gtgtagcaaa
     1561 gctgaatcct ctaaagacat gatctcctct tcgtcctact agtgcttgta caccacctgt
     1621 attcggatct aaagctactg aagcagattg gaccatggca ccatctgaag catttgctgg
     1681 gaataatgca ctgttttgat aagtagcttc taattgcttt tgataatcct gatctaaagc
     1741 tgtatagatt ttatagcctt tattcatgac atcttcttca tcaagaccat actcgttaac
     1801 tgcttcatcg atcactgcat caaaataata aggataacga taaccagaat ctgaattatc
     1861 gtatgtgtca ttaagatacg cgctgatatc tgtctggctt tctgcttgtt cttgttcttt
     1921 tgtgattttt ccgttttctt ccattacact tagtactgtg tttttccgat cattcgcatt
     1981 ttttgggtga tcgatcggat tataaatacc aggacctttc agcattcctg ccaaagtagc
     2041 agcctcagac agcgtaacat tcgttgcatc tactccaaaa tactttctgg aagcgtcttg
     2101 gacaccccat accccgttgc caaaataggc attgttaaga tacatggtca agatttcttc
     2161 tttgctgtac ttcttttcga tctcgattgc caaaaataat tccttcgctt ttcgatcaaa
     2221 cgtttggtct aatgtcagat acgcattttt ggcaagctgc tgagtgatcg tacttcctcc
     2281 gccccctgat gttccgccag acaagaccat tcggactgct gcacgggcaa tccctttgat
     2341 atcaaacccg tgatgctcgt aaaaattcct atcttctgtc gagattactg catcttggat
     2401 atagggagaa atcgcgttta attctacata tgttcctttt tgggcgtaca gtgtgccagc
     2461 ttcttcgttt ttcttatcat aaatgactgt cgattggctt aatcctgatt ttaacgtttc
     2521 gacatttgct tgttttgcta aaataaagag gtagatactt gtcactaaga cacagatcaa
     2581 accaatcagc aaaatcaatt tgttcacatg atattttttc cagatgcgct ttcgccatcg
     2641 atggaattgt ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag cagcttttat
     2701 cttttctaaa atgctttgaa aatccatttg cttcttctcc tgtctatttg attcaatcgt
     2761 cttattttac catagtgaaa gagtgttttc ttcctgctta agttgtagtt caatatgttt
     2821 ttttacgtta gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa gttttttctt
     2881 aaatatgttt taaaaatggt acaattagtc tgcgattgca ctgaaaggag tacacttgtg
     2941 aaaatcacga tcactttacc tatgaaccat cctgaaacaa caattcgcga cttgcttgaa
     3001 aaggaatggt tagttcctag aaaggttcga cactttttgc gtatacgtaa aaacgtttgg
     3061 atcaatcaac aacctgcttt gttccatcat gaagtaaagg gcaatgacca gatcacattg
     3121 ttattcgaag aaacagatta taattatcaa gaagttcaat taggagatgc gaagcacatc
     3181 cgtgttttat atgaagatga gcatttgatt atagtggata agcctgcagg aatgaagact
     3241 catccaaatg aaccgacaga aaacagtacc ttactcaatc atttagctgc ttatctcgac
     3301 actaaacagc aacgacctta cgttgtccac cgcttggaca aagaaacaag cggtgccgtt
     3361 ttatttgcaa aaaatccatt tgtcttgccg atacttggaa gaatgttgga acaaaaaatg
     3421 atctatcgga gatatcaagc catcgtatgg ggagaaattg ccaaagatat gaccatcacc
     3481 aataaaattg gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga taaaaatggt
     3541 cagagcgcct tgacacatgt cactgtcaac aaagtacttt ctgaacaaac acaagtctat
     3601 tgtgttttgg aaaccggacg tacacaccag atcagagttc atttagctca taagggacat
     3661 ccgatcgttg gagaccctct ctaccaaaat agaacagctg aacgactgat gctccatgca
     3721 ttagaactgc acatgatcca tccatttact caagaaaaaa tagtcggtaa agctttacct
     3781 ggattatggg aatagaaata aaaaagaaca gagtcttatc agcagataat gataagactc
     3841 tgttcttttt gtgtaatttg tctgcttact tttgatgttc gtgttctgct aacagcaaac
     3901 taccgataat cccactttca ttcggcagtc cccaacgaac gatatattca cttacttgag
     3961 gtgtttccat ataaccaccc atcaattcaa caaattgctg acggattttt tgaaggagat
     4021 gatcctgatt cattacccca ccgcctaaaa taatcttttc aggtgctaac gtcaacgtat
     4081 agttgatcag tgcttgagca atataataag cctgaatagc ccaaacagga tgatcttgcg
     4141 gcagatcttg ccctttgatc cctgtgcgtg cctcaataga cggtcctgct gctaatcctt
     4201 ctaaacaatc tttatgataa ggacaagtac cttcataatg gtcatctgga tggcgtttca
     4261 cccaaatatg tcccatttca ggatgtgcga tccctgaaaa aatttcttca ttgatcacga
     4321 cacctgcacc gattcctgtt cccacggtaa ggtaaataca actatttttt ccttgtgctg
     4381 ctccttgctt caattcccca tatgcagcag cattgacatc cgttgtccaa gcagcaggaa
     4441 tattgtagcg ttccttgatt gtgccaagaa aatcgaattg tgcccaccct tttttgggag
     4501 ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat accaatcggg ccgaatgagc
     4561 caatcccgat tcccttcaat tcataacgat caaaaaattc aaatacctga tccaatgttt
     4621 cttctggtgt tgtggtaggg atacttaatt tttctaaaat ttctccacca tctgaaaccg
     4681 cacacacaaa ttttgttccg cctgcttcta ttccgccata cattcgtttt cctcctcgaa
     4741 tccgattgtc cgaatctcat aaggttggat tattgggaca accgcttgct tcgttggttc
     4801 ttcaagcaga ttcaatatga cagtttttcc attgtcttta gttatttcca atttttctaa
     4861 atgactagac atattgaaac ctcttactac tactttatca ctaaacttgc tgcgtttcaa
     4921 agctgtaatt gcaaacgttt cactttttat cgttaaatat tgttgttttg aaatcaatgt
     4981 tccttcatga tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt atgcatgcaa
     5041 ataagtagag aatttttctt ctggtccatg tagttcaatg ctgtaattga accgatgctc
     5101 acctagacac tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt ctcccacact
     5161 gcgaagtagc gtcactgcga tttgtccgtc ttctgttact tcatattcat tcaaaccaaa
     5221 gtttccgact gttacaccgt actcttcatc atgaatattg acaaatgctt gctgatgttg
     5281 cggattcgtg gggttttccc aacttttaga cacatggttt gggcgcttca ccacttcaaa
     5341 aatactatct gcttcgtgat gttccacatg taactttgta ggaaacaaga cacgtaagcg
     5401 atgatctttc atctgattgt cgagagtcgt ttcaaagtcg atttttttac tatcttttct
     5461 gattgtcatg attgttttta tttgaaggat tcgtttttct ttggatcgtt ctgcttttcg
     5521 ataacgaaat tccacaacca tctgctgttc tttttctaaa cgctcgtctg cagaaacagg
     5581 gatttccatt tcctgtagaa tacttatttt tgctatttcc ggtgtatcta ctaatacttt
     5641 tacttcagag ttttctttgt tactggatag gattggctga tcacagaaag gttgtttaaa
     5701 gatatactca tttcctatat cgccgatatc ttcaaaaata tgcagattat tcaactttcg
     5761 tttgtttttg cgatcttcca ttgtaatggt accatttttt tcaatcgtca aatgtagagg
     5821 gccgttttcg atgattcgtc cactctgatg aatcattgtt tccttttctt ctgctttcgc
     5881 ttccccttct acaagcgcga atgtttccca agaaaatgct ggcatgtttt ctaatggtaa
     5941 agtcactttg atcattcttg ccatatatgg cacacggaaa cggtctttcg gcaaatcata
     6001 accaaattgg acagtttctt cagataaaat agctggtata gtggccccac tttttgtttc
     6061 tacatgatac ttacgtttag gcaaattttc taactcttga tatagttgtt caggtattcc
     6121 ttcagcgaat ctttttcttt ctaattcgat ggttatttct gcttcaccag ttttatccat
     6181 tcctgcagta tttacaatga caaatggaaa gctgtctttt gggaatccgg tcgtatcgat
     6241 tgctgcggtc agctgttcta aggaatcttg tgcaagatac tttccaactt catctgcttt
     6301 ttcaaaacga ggtatcattt cccgatggac agaatcgact gaacaaccgc aaatactgtc
     6361 gtgaggatga ttttgcatta atgtcttcca agcataatct aattgatcat ggggatagtt
     6421 tccactaact tcataagcca tcgtcgcaag tggttctgta atattttcta gctgtcgctg
     6481 gaccttggta ttccattgtt ttaagtaaac ccgagcagat gctgtattgg ctaaagtata
     6541 ccagccatcc gtttcttgac ttgtcaattc tccttctact gagcctaagt cttctggcac
     6601 atctttttga actgcttcca gatagtcagt aaaattagaa tgaataaact cgtaatctgg
     6661 aaacagttca tttgcgagat ggattgcttt tgatatatct ttttgtaccg gttgatgatc
     6721 aacaccattc atcatcaata agtggttcgt agaggcatat tgttctgcat ccgctaattt
     6781 ttgcttccaa aaggcaagag ctgcttcttt ttctactgga atctcattcc cgttgctata
     6841 ccaattggca aatagcagac caaagattgc tgtttgatca ggtcctttcc accacatctc
     6901 tgagtattgg gaagaatagt tttctgcttc caaaacttga ttatcaaagc caattggttt
     6961 tacaccacgg ccaaacgctg ctgctgaaat accagcttgt ttcattaatt gaggcgtctg
     7021 tcccatattt ccaaatgtat cgggaaaata gcctaacatg acaggggttc cccatttacg
     7081 gctttcttcc atgccaatca gcatatttct cacgttcgat tccgagctga taagaaaatc
     7141 atcttgcaag atataaaatg gtccaattct caattttcct tcattgattg cttgttggat
     7201 tgcctgtctt ttttctggcc ttacttgaag atagtcatct aaaataatcg tttgcccatc
     7261 caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc tctagtagat cgtctatcaa
     7321 ttcaatcagt cgcatgtgat gctgttcgta agccatgtac cattcacgat cccagtggct
     7381 atgagaaatg atatatactt tctttttcat ttacgatacc tcctactttt ctactctaat
     7441 atcaaagtaa tccatgacta attcgcaaaa catcatattt gcccaagaaa accattctct
     7501 cgtataattt tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg ttcctgcgtc
     7561 agttgctaca agtaaattca atatccgctc tttttcagat ttgtctgacg ttgtcattcc
     7621 ttccatagct aatgcaatcg gccaaacgta tttttctggc gtatgggagg atccaattcc
     7681 ttttgcgtac tttccttcat agaaatatgg attttcttta ctcaacaatg ttcttcttgt
     7741 ttgcagataa tcttcctctt gttcagaaca atatcccaag taaggtgcgg cgatcagatt
     7801 gggcacatta ctgtcatcca tgactgtggc gtttcccatt ccatctactt catatgcata
     7861 aatcgtttgc cctttttgat taaccgttcg tccgtgattc tgaatcccct cttggatctc
     7921 tttcttcaat ttctgtgttt gctgtacaat ttctttatca tcgagtatcg tttgaaaaat
     7981 ttcttccaca taacctaaca cgaccaccgc aaacatattg gatggtacca gatatccata
     8041 acggcaagta tcatcgctag gacggaaacc agaccatgtc attcctgtaa aagctgtttt
     8101 tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc gtacaaatgt
     8161 ataaggagag tgttcatgat cttgctctgt tttgaacacg tccaagattt ttttgatacc
     8221 ttcaataaaa gattgattga attgatcagt ttttcctgta tttttgtaaa gaagataggc
     8281 caactgtatt gggtagcata aagaatcaat ttcatatttt ctttcccaga tccaatcgtt
     8341 catttttgta tgatctgttt ggtggccagc accgttagct tcttcgttga aggcattcgc
     8401 atatggatcg atattgatat aaaaaaattg ctttttgact aacccgctga tcatgtcagc
     8461 taattcctca tcttcttttg caatcactaa ataaggacga acttgtgcag tagagtctct
     8521 taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat tttgcttttt
     8581 gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc agtccgcatg
     8641 gatatcccca catttttgtg tgatatcttc cataaatttc tgcaccgatt ttggtatatc
     8701 tttataagcc actaataaaa ccttctttct atttgatata tctttaaaga ttatacttca
     8761 tacagccaat tatgtctacg ctttcaaaaa atatcttttt atttactttt tcatccatta
     8821 tggtattatt tgaatatgat atatcattaa gaggtgttta tatgaatcaa ccattgtacc
     8881 agacaatatt taatgatttg caaacggcta ttttaactgg tgaattacct gtagacagcc
     8941 aactaccaac tgaaaaagaa ctttcacgta cctataaagt aagcagaatc acttccaaac
     9001 gtgcattgac tgaattagag caattaggac ttatttatcg cgttcgcggg aaaggcagtt
     9061 tcgttaaatc cgcttctccg gaacagattc ttgcttctcc tacaaaaaca cgacggattc
     9121 tgtttcttct tccatatgtc gctgatttgt ctgttggtga ttttagcaag ggattgaacc
     9181 ctgtgatgca agataaacag attgatgtca tgatgacgac gcttgacttt ctacaaaata
     9241 aaacagcaaa agagctaatc aatgaatttg acggattgat ttattatgct cttgatacag
     9301 aacaacatct tgacttactt tttgagcttt cattgaaaga attccctgtc gtcatattag
     9361 ataaaaaaat ctatgagtta ccttttccta ccgttctttc cgataatttc caaggcggtg
     9421 cgcttgctgc ctcttatttg atcgaacagg gacattcaaa aatcgcttat ttgtttggta
     9481 cgcagactca tccacaatcc gttcgacagc gttaccttgg ttaccttgaa gcattggaca
     9541 aagctggttt gactttccac acttcactgg atgacaaaag cgcgacatta gatgaattgc
     9601 tttcttatat cactaagtat gaagtaactg cttttgtttg tgaaaacgat gttgtcgcta
     9661 tccagaccat gaatctcttg agacagcatg gttatcatat ccctgaagat ttttcaatta
     9721 tcggatttga tgatatccaa gcagcagctc ttgttgaccc gccactgaca accatcgcac
     9781 aagattttga acagttaggt aagcttgcgg gagatgcttt ggtcacttgg ttagagacaa
     9841 aagatcgacc atccgatagt aaatttccag tttcattcat caaaagacaa tcaacaaagg
     9901 agaaataaaa tgaatatcgg tcaaatagat actagacatg caacagcgaa tcagccaaat
     9961 ttctctaatg ggaactgcca gccttatact ggcgttcctt ttgggatgaa ttattttgca
    10021 ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg cgtttttcaa
    10081 ggctaccgtc ttacccatca accaagtcct tggatgggtg atttcagtca tatgctgttt
    10141 acgccgatca atggaaaact acaggagaac acccttttcc atgctcaaag ttcttaccgc
    10201 ccagaagaaa gtatattttg cccgactcat ctgtcgatct gtcaattacg ttatggcatc
    10261 tgttctacat tgatacctag catgtatggc ggaatcttat ctattgatta cagcaaaaat
    10321 gacagcggtt tacttttatc ctttccggga cgccatcagc tctttgttat agatccttac
    10381 acagtggaag gaaaaatcac acattttgct ggttcagaag atcctgattt ttctttttat
    10441 tttatattga gatttgaata tcctttaaca attgatgagt tagctgtttc caacaaaaat
    10501 agcgactcga tcccgttgta ttttgaacag acaaaaaaac aaactatccg atttgggaca
    10561 tcttttatca gctcggaaca agcagcttat aacctggaac tagaacaaaa ccaaagcaaa
    10621 gagacttacc ttgctcacag tcaagaacaa tggcagtcct attttgatcg tatacagctc
    10681 acacaccatg atcaacaaca attgcggacg ttttatcata atttatatcg ggtgttttta
    10741 tttccacaaa cattttatga aattgatcct agtgggcaaa aaatccacta tgatacgact
    10801 agtcgatcta taaaacctgg tgttctctat acaaataatg ggttttggga tacgtataaa
    10861 acggtttatc ctctttattc tttgattgct gtagaaaaat acgaagaaat gctggaaggt
    10921 tttttgaaca gctatagaga aagtggctat ttgccaaaat ggctgtctcc tgatgaacgt
    10981 ggacttatgc ctgggacatt gatcgatgcg gtcattgcag atgctgctgt aaaagatatc
    11041 cgacgagact tgatgcctga atttctaacg gcaatggaaa aaggtgctac ggttcaaagc
    11101 aaacatccaa attatggacg ccaaggaaca gaagattact tgacatatgg ctatgttcct
    11161 aatcactacc acgaatcagt caatcacaca ttagactatg cttatagcga tttttgtatt
    11221 agccaagttg ccaaggtact agaagaagag gaaatgacga agaaatatca gcagcaatca
    11281 ttgaactatc aaaatatctt tgactatgag tcaggattta tgcgttcaaa agataaagat
    11341 ggcaagttca gagaaccatt ttcagctaat cgttggggac aagattatgc agaaggaagt
    11401 gcttggcaaa gcagctttgc tgtttatcac gatttttcag gactgatcgg aaaatatggt
    11461 ggcagtgaga gatataaaga aaaactcatt gagctgtgca atcagccccc aacgtttgac
    11521 ccaggaggat atggttttga gatccacgaa atgagtgaga tggccgcgat agaatttgga
    11581 caattagcta tttcaaacca gccaagtttt cactatcctt acttgttcag ttatatagga
    11641 caacctgaaa tggcacagcc actgcttaaa cagttgatga ctcagacctt cgacgattct
    11701 ccaatgggtt accctggaga tgaggacaat ggaagtatgg ccgcttggta tatcttcaat
    11761 agtctgggat tttatcctgt cacgcctgga accggtgaat atgttatcgg tatgccatta
    11821 attgacaagg ctgttttgct tttgtcaaat ggaaaggaat tgacgattga agcaacgcct
    11881 aatcggccgc agcaacaatt tatccataaa atcaaacgga tggacaaaga atatacgaaa
    11941 ttatatttta cccatcaaga tttgctgaaa ggcggcacga tctcttacca attagggatc
    12001 atccctcacc ctaagaaata tcaagcaaaa gatttgcctt tttccttatc aaactataat
    12061 taac
//
LOCUS       DACEMO010000075        12056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEMO010000075 DACEMO010000000
VERSION     DACEMO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.077"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            912..2162
                     /locus_tag="IW728_001540"
     CDS             912..2162
                     /locus_tag="IW728_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ1024905.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            2181..3191
                     /locus_tag="IW728_001541"
     CDS             2181..3191
                     /locus_tag="IW728_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ1024906.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDVHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFEDGGTRVIHYTPETYIEKQ
                     NG"
     gene            3201..3854
                     /locus_tag="IW728_001542"
     CDS             3201..3854
                     /locus_tag="IW728_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1024907.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3873..4871
                     /locus_tag="IW728_001543"
     CDS             3873..4871
                     /locus_tag="IW728_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1024908.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWKPTVSLEEGLRESYNWIEKQIKG"
     gene            4947..5708
                     /locus_tag="IW728_001544"
     CDS             4947..5708
                     /locus_tag="IW728_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ1024909.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5726..6832
                     /locus_tag="IW728_001545"
     CDS             5726..6832
                     /locus_tag="IW728_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1024910.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRDVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6822..7055
                     /locus_tag="IW728_001546"
     CDS             6822..7055
                     /locus_tag="IW728_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024911.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            7254..7709
                     /locus_tag="IW728_001547"
     CDS             7254..7709
                     /locus_tag="IW728_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ1024912.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7723..8496
                     /locus_tag="IW728_001548"
     CDS             7723..8496
                     /locus_tag="IW728_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1024913.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8483..9613
                     /locus_tag="IW728_001549"
     CDS             8483..9613
                     /locus_tag="IW728_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1024914.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9644..10879
                     /locus_tag="IW728_001550"
     CDS             9644..10879
                     /locus_tag="IW728_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024915.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFVPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLSVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10904..12013
                     /locus_tag="IW728_001551"
     CDS             10904..12013
                     /locus_tag="IW728_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024916.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAKYEKYMDFVWEE"
BASE COUNT         3838 a         1766 c         2734 g         3718 t
ORIGIN      
        1 atatatatgt atatggtgac tgaagacaaa aaaacagaac taatgaaact gaataaaaat
       61 atagagtgaa ctagtttcta agagggattt taaactagat ttcattggta ataaaatttg
      121 gagagatgga aaaacagcac ttaaggaagc agattttgtt gtcatttttg ttcctacaaa
      181 ctatggtagt gaatgtgtta ttgaattagt gatgtctgtt atttcagagg cattggggat
      241 aattaaatcc actatacctg taggatatac aaaaagtgga agaaaaaaat attatcaata
      301 gattttattt agttcagaat ttttacgtga aagtagatta ttaatgataa tctttatcca
      361 attagaatta ttatcgtagg atgtgatgat gaaagccgtg aaattgttca agtatttacc
      421 aatgtctccc tgccagtaaa tccgcttaga atttgagcct tgaaaatgtg caatgataaa
      481 aaacaaaaac tcgctcaaaa tagacagttt ggggtgggaa tagaccaata atgtaccaga
      541 caaacttcca tttggggtga aaataggtca ataaagcgat gttaaacata cctttggagt
      601 aagaatagag ggtaataagt gaatttataa actttttggt gactggttga gtggaaaatg
      661 taggtacatt tcaatgcgaa aatgtattat aaccccaaac gaggtcaata aaaatgactc
      721 aaacttgcaa tataacccta aacgggtagg ggatttatga aaaattgaat ttgccggcgt
      781 aggagctggc tgttttaagt atgaatgaac acagctttcg ggctgtttta tataaatagc
      841 aggttactgt tcacaaacag cattttgtta agaaagtagt gaccacagca gtggtggaaa
      901 gaggaaatat tatgaagaaa cagtataaca ttgcagtcgc cggcaccggt tatgttggtt
      961 tgagtattgc cactctgttg gcacagcacc ataaggttac agcggtggat atcattccag
     1021 agaaggttga actgattaat aaccgtaaat ctccaatcca ggatgattat attgaaaagt
     1081 acctggctga gaaagaactg aatctgaccg caacattgga tgcaaaggca gcataccagt
     1141 ctgcggattt tgttgttatt gcggctccta ctaactacaa tagccgcact caacattttg
     1201 acacatccgc tgttgaggct gttattaggc tggtcatgga agtaaatcct gatgccatta
     1261 tggttattaa gtccaccatt cctgttggat acaccgcttc tgttcgtgta aagactggta
     1321 gcaagaatat tattttcagc cctgagttcc tgcgtgaaag taaggcactg tatgacaatc
     1381 tgtatccttc gcgtattatt gtgggtaccg atatggatga tgagcgtttg gttgaggatg
     1441 cacacacttt tgctacactg ctacaggaag gcgcaatcaa agagaatgtg gacacattgt
     1501 tcatgggctt tactgaagca gaagctgtta agctgtttgc caatacatat ctggctcttc
     1561 gtgtcagcta cttcaatgag ctggacactt atgcagagat gaaaggcttg aacactcagc
     1621 agatcattaa tggagtttgc cttgatcctc gtattggtac tcattacaac aacccttcct
     1681 ttggttacgg tggctattgc ctgccgaagg ataccaagca gcttctggcc aactacgcag
     1741 atgttcctga aaatctgatc gaggcaatcg ttgaatctaa ccgtactcgt aaggacttta
     1801 ttgcggatcg tgtacttcag atggctggtt attacaatta cgcagaagca aatcagtatg
     1861 atgctggcaa agaaaagcct gttgtgattg gtgtttaccg tttgaccatg aaatccaatt
     1921 ctgataactt ccgccagagt tctatccagg gcgttatgaa gcgtgtaaaa gctaaaggtg
     1981 caactgttgt gatttatgag ccgaccttga aagatggaga gactttcttt ggcagtaagg
     2041 ttgtgaatga tttggaacag tttaaggcaa tgtgtgattc tgttatagca aatcgctacg
     2101 atgcttgcct tgatgacatt aaggaaaaag tatatacccg tgatatcttc cagagagact
     2161 aagattagga gagagtattt atgattatta caaaaacacc gtttcgtatg tctttttttg
     2221 gcggtggaac ggatatggag gattatttca aagagaatgg cggtgcagtt ctttctacta
     2281 cttttgataa gtactgttat gtgaatgtga gacatctgcc tagatttttt gattacagcg
     2341 ctgagttatc ttattctcgt actgagagag taactagcat tgaagatatt gagcatcctg
     2401 cgattcgtaa tgcgatgaaa atgctcgatg tgcatgaaat tcggcttaca tacgaagctg
     2461 atttacctgc tcgctctggt ctgggtacat ccagttcttt tgcagttggt atgttgaatg
     2521 ctttttatgc actggagggt aagtatgctg acaagaagaa acttgctgat gaagccattt
     2581 atcttgaaag agttctgtgt aatgaagccg gtggatggca ggatcagatt gccgcatctt
     2641 atggtgggtt caatcgaatt aatttcaatg tggatggtta tgaggtgctg cctgtcatta
     2701 tttcgcctga acgtaaaaag cagcttaaca acaatctgat gatgttcttt accggattca
     2761 ctagattttc ttctgatgtt caaaaggcta acaacgtcag tggcacagag gataagagag
     2821 taagactgaa aaagatgtat gagcttgtag atgaagctga ggcaattctt actgataaga
     2881 acagaaattt agatgatttc ggtagacagc ttgatgttac ttggagattg aaaaaaggta
     2941 ctggtggagc aattagtaca ggaagcattg atgaactata tgaaaagggt atggctgcag
     3001 gtgctttagg cggaaagttg ctcggtgcag gtggtggagg tttcttggtt ttctatgtac
     3061 aacctgagaa acaggatgca gttcgttggg ctatgcgtga tttgatgtat attccgtttg
     3121 aatttgaaga tggtggaact agagttattc actacacgcc ggaaacttat attgaaaaac
     3181 agaatggata aggagtaaca atgttagcgg aaaaacttaa taaacatatt gacttattga
     3241 ttaaacgata tcctacactt gagacgatta aacaggacat tatcgatgga tacctaatta
     3301 tggaggaatg ctatttgaat ggtggtaaac ttcttgtggc aggaaacgga ggatcagccg
     3361 ctgactcgga acatattgct ggcgagttga tgaagcggtt taagacgcca cgtcctgtaa
     3421 gtaaggaatt tgccgataag ttagttgcaa ttgaccctgt aagaggtccg gaattagcca
     3481 aaaatctcga gtgtggttta acggcaattc ctttggtcgc acacgaagca ttgtctactg
     3541 cttatattaa tgatgtcgat ggtttgggtg tcttcgcaca gcagctgttt ggctttggta
     3601 gatctggtga cgtattcctt ggtatttcga cgtccggaaa tagcaagaat attatgagcg
     3661 caactgttgt tgctagggct ctcggtataa aagttattgg cttgacgggc gcaaagggtg
     3721 gggaacttgc cacggtagca gatgtttgtg ttaaggctcc tgaagttgaa acatacatga
     3781 ttcaagagta tcacttgccg atatatcatt gttggtgttt gatgctggaa gataaattct
     3841 ttggaaacga ataaaatttg gaggaatgtt aaatgaaaaa gacaattatg gttaccggtg
     3901 gttgcggtat gattggctcc aatcttgtta agagactggt gaaggaaggc tgctgggatg
     3961 tttatgtagc tgataatctg tggagaggaa agttggagta cctgaacgac gaagatgggc
     4021 atcctgtcat cgacctggat actcatttct tcaatgctga tctgactgat tatgagcagg
     4081 ctaaaaaggt tattggtacg actgagtatg tagttcatct ggctgatgtt gtggctggca
     4141 ttgactatgt gtttaagaac cagggcgaat tgttccgaat caataatctg atcaattcta
     4201 atgtttttga ttgttgccgt aaggctggca aggagaaaat caagggtgtt atctatgttg
     4261 gtaccgtatg ctccttccct ctgaccagac agaatacttt gaatcctgag cctctccggg
     4321 aagaggagtt gttccctgct cttcctgagt ctgcttatgg ctggagcaaa ctgatgggcc
     4381 agttggagat ggggtatttg gaaaaagaaa ccggaattcc gtgctgtatc ctgatgcttc
     4441 ataatgttta tggcacacct actgattttg gcgaacgtag tcaggttatt cctgctctga
     4501 ttcgtaaagc aatcaataat cctgaagagc cttttaatgt gtggggtagc ggagagcagg
     4561 gacgtgcttt cattcatgtc aatgatatcg taaatgcatt ggtgctcgcc ctggataagg
     4621 gttggggtca tggtcatatt cagattggcc cttctcactg tacttccatc aaggaaattg
     4681 catataagat cattgaaatt tctggcaaag acatcaagcc tttctttgat acttctaagc
     4741 ctgaaggtga taaggctcgt tgcgcagatt attctaaggc gaaagagatt ttggattgga
     4801 agcctactgt ttctttggaa gagggtcttc gtgaatccta caattggatt gaaaagcaga
     4861 ttaagggtta ataagtatat aagctgttct tcctccgtgt aattagagga tgcggaggaa
     4921 gaacatatga gtaaggatgt actgagatgg aaaagaatat gtataaaaga aaacgtgatt
     4981 tcactgtgtg tggaatagta ggtgtttgtg ctactacaaa gaacgagttt gtaagggaag
     5041 ttttttccat gccaacatct ccggtaagtg cgactgttga tctcgtggga gtgccggcca
     5101 ttatttcggc aaaggaaaat cccaaaattg ctgagatgta tagccaggct acaatggctg
     5161 caatagatgg tatgcctatt gttaaaattg gacggcgcaa aggatttacc tgtgaacgat
     5221 gtgctgcacc agatattatg ggaatggttt ttgaagaagg cgttagacag ggaaaaacac
     5281 attatttcta tggtggaaaa gataacgaag tgttaaataa gcttcgtgca aacctagaaa
     5341 gagattaccc gggaatcaaa attgttggaa tgtattcccc accatttcgt ccgttaaatg
     5401 aagaagagga tagcaagatt tgcgaggaaa tcaacaactt aaatccagat tttctgtggg
     5461 ttggtattgg tgcgcctaag caagaaatgt ggatgtggga acaccagaag aagatccata
     5521 acacagtaat gttcggtgta ggcgccggct ttaacttttt tgcgggcact ttggaaaaag
     5581 ctcctgaatg gatggaaaaa gctagtctag agtggttatt tagactggta aaggaacctc
     5641 gtagactatg gaggcggtat atcttgggag gtataaagta tatctactat actattgaat
     5701 ctaagtaggg gggcaataaa aatagatgaa aatacttata gtagttacaa tgacgaataa
     5761 ctttggtaag aaggggtcct ataattctca ggaaatagga ttagctcgag ggttagctga
     5821 attaggccac gaagttattg tatataaaag tgtaaaaagt gatgaggaag ttggaacaga
     5881 gcaattaagc aatacggcga tgattcacta tatcaagtgt cgttatgtag gatcaaatgg
     5941 attcttatca acaaagaatt ttgataagaa tgcggatgtt ctaatacaat ttgcggacct
     6001 tcaactatca gtacctcatg tatactcatg gactgtaaaa aatggaataa aatatatacc
     6061 ttataatggg ataacgcgaa gtcaaagtgg caataaatat ataaagacaa ttattgaaat
     6121 attggcgcat cgaaattata aaatttataa gaaaaatggg tgtgttgcca aaaatgatcg
     6181 agtttctgaa aaattagtac aattaggagt aaataaagtg aaacttgctc ctgtttgtgt
     6241 ggattttgaa cagttacata agaattacga agcaatagat tttactactc tcagagaaaa
     6301 atggaatatt cctcgcgatg ttcgtgttat tttatatgta ggacggtttg aaaaggataa
     6361 aagaccgtta gatatgatta gtttatttaa taaacttgat gagcataatt atttgctgat
     6421 ggtaggaact ggtgctttac tcgagcaaac gaagcagcag ataaatttac ttggactgca
     6481 gaatagagta aaaataattg agcgtattcc taacaaagat atttgggagt tatattgctt
     6541 atcggatgta tttgtaaatc taaactatac tgaaatttgg ggtatggttt tgctagaggc
     6601 aatgtactat cgcctcggaa ttattgctgt taatgcacct ggcccacaga atattatttt
     6661 ggataataga tatggaatgt tggctaaatc tgacgatgaa attttgacat tcttaaaaga
     6721 cggacgattt accaacgata tcatagatgc agcatatcag cgtgtaacta cagaatttag
     6781 atggtcaaag tcgggagaaa ttatgcttag catgttggaa ggtgataaat gatatggaag
     6841 taacgaaatt aatggcattg aggaatcgat atgctttaaa catagttgat aattgcacga
     6901 gaaaaatagc caaaattctt ggttgttgta ttggtaaagg tgcacagata gggaactctg
     6961 ttgaatttgt tcataattct gtaggcactg tgattcattc tgacacaatc cttgaagatg
     7021 gtgttaaagt ttatcagaat gtgacgtgtg gttgagcaga tagtaatgaa cgtatcatca
     7081 ttcgagtaaa aaagaacgct gttttatgtg tgggtgctaa aattctttgt aaatctaaca
     7141 cagaattatg cgttggggaa ggtgctgtag ttgctgcaaa tacagtggta ttgaggtcta
     7201 ttccacccta cgaagtgtgg ggggtacctg ctaaaaagat ggggagataa aacatgaatg
     7261 catataaatt atacaaagta gaacgatggt tttacttaca caaaatggag ttttttgcca
     7321 agattgtaaa ggcactaatt tatgtacttc ataatagctc aatttcatat caaacagaaa
     7381 ttggagataa ttgtaagttc ttatacggtg gcataggttg tgtaattgga aaagatacaa
     7441 aaataggtaa tcacgttata atcggaacga atgtactgac tggtggtaga tccaataagc
     7501 ctggtatgcc tacaattgga aacaatgtat atatcgctac tggcgcaaaa attttaggta
     7561 atatcacaat aggggacaat gtaatagtag gggctaatgc tgtggtcatt aatgatgttg
     7621 aacctaattg ttcggtagga ggcgttccgg ctagagtatt gaaaaaagat attgatgtaa
     7681 atgattattg tagtttgaat cgttactaaa aggagagatt gtatgttgta ttttgttatt
     7741 cctttaatga gtgcgaaggc agcaaacaat tgggagatgg ttagtacctt atttaaccga
     7801 acattatggt cgtgttataa tcagactgat cctgatttca aaatcattgt tgcttgccat
     7861 gaaattccta aactcacgca tgaatatgat gacagagttg aatttattca agttagcgaa
     7921 aaggatgccc ctattcccac aaatcaagac gaaaaaatga ttgataaggg ttataaaaca
     7981 catgctctcg caatgcgttt gcgagagttg ggcggcggtt tcgctatgat ggttgacgca
     8041 gatgatcttg taagttctca tttggcggaa tatgtgaatt cccatcccca ggaaaatgga
     8101 ttttatgtca aaacgggata cgtgtacttt gttggagacg attatatgaa ggttctacca
     8161 aagttctcat caggaagtgc gtgtatagta aaatatgcag tggaagacct gcctgaggca
     8221 tatccggaag ttatgacagc aaattgtgat gataatccat ggatcattag aaaacgacat
     8281 gggggcgttg ttccagcatg tgaagagcaa gggcgtccat taaaacctct tccattcaaa
     8341 gcagcagtat atgtattagg aacaggggac aatcactcgc tttatggtaa aacgacaaaa
     8401 tatcagacga agatgcgaga gatactggaa atgtttatag caaaacgtaa aattaaagga
     8461 aaactgaaag cagaattttc tgttgattgg ttataaggtg agtattatga aggtattgat
     8521 gtgtgaaagc caaatgccag agaattggta ttcgtttgat ctttctaaag aacttaatca
     8581 atatgttgat ttaacattgc tgtgtaagcg tgatgttcct cctttagatc ctaaaattaa
     8641 gagcaaacat attttgtatc ataaaagtac aaataaagtg ttttcggcac ttgtttttct
     8701 gaaaagcttg ttcttaattt ggaaggaaat aagatttggt gattatgata ttgttcattt
     8761 gcaattgatg aaaagtctca aatatgaagg cggattgtac gttttatgta agagacacta
     8821 taaaaaatta attatgacag ttcataatgt gttaccacat gaggttcgtg aaaaggatcg
     8881 ccaagagcac tttaatatat attcaaattg tgatggtttc attgttcaca atcgagtaac
     8941 agaacagcat ttatatgctg aattcccaga attaaatgca aagtcatatg tttgtccaca
     9001 tgctatctat ggagatgtta aacatatatc tagagcgtat gataaagata agtttgaatt
     9061 tatcttgttt ggacaaattc gtccatataa gggaattgat gtactgctta atgcaatcac
     9121 agaacttcca atggatgtaa gaaagaaagt tcattttacc atagcaggtc aacaacatcc
     9181 taaacaggat agtactgact ataaaaaaat gattgaagat ttaaaggttt cagattgtgt
     9241 tctatataag ccagagagaa tttctgatga agaaatggac agtatgttca gaaatgcaga
     9301 tgccgcaata tttccatata aagaaatcta tggaagtgga gcgcttctta tggcatacac
     9361 ttattgctta ccaactattg ttagtaatgt acctgctttt atggaagaaa ccgaggaagg
     9421 aaagataggg ttacttttcg aaaatttgaa ttctaagagc ctagcagaca caattgtaaa
     9481 gtatgtgaac cttagcccag ctgagaaaag caaaatagag gatgatatat atgagcttgt
     9541 aaaccgcaag tatacctggg cggcatctgc aaaactcaca tacgaggcat acaaacaaat
     9601 tttgaatgag tagtaaaaca attagaaagg aataataatt aacatgcaga ttagtaaatg
     9661 gaaaatcata cagtttctaa tggtctttct aatgattatc tcatcaagca tattgaagcc
     9721 tttgggcggc tggttgggag aaacgatgaa cgttagactt gtgacagtca ttctagcaat
     9781 agtatgtttg ttaatagcct cattaccagc atttagaaaa aatagatatt taaatttctg
     9841 cactttactg atttgtgttg ttataatagc agcttacatc tactcagata agacaatatg
     9901 ggttaaatct agggaaagta tgagtttata ctatgtatta ttggcgtatc cattgttctg
     9961 tactttaatt aaccaagcgt ggaatttgga ttcaatgcta aatacgatat gtggtgtaac
    10021 atttctttca tacattctta gaacatctat ttcacttatt cagaaatttt caggagtact
    10081 tctctatgaa aacatatatc gtgagtgtgc cccggaaaat tggtatcgtg gaggcttttt
    10141 aagaattaat ccaccatgtt ttacaattat aataataccg attgctttgt atatgattga
    10201 gcgacaagtt gtagctagaa gaaaagtaaa atattattta ctgattgcta gcgcactggc
    10261 atattctgct gttattcatg gttctagatc tgtccttgtc tatcaaatta ttgtacttgg
    10321 ttgctatatc ctttttaaga aaggttcttc gaaaagaaaa attgcaatgt gggttttagc
    10381 tgggttgctt gtagtcatat ttattaattc gaccatgttc tcaacatttg ttgaaacttt
    10441 cacaaacagt acaggtgaat atgcaggatc tgctgaatct cgatttgctt cagtttggtt
    10501 ttttgttcct aaatttttgc aatcgccttt gttcggtact ggtattctta ctggtgagga
    10561 atttacgttt atattaccag gaggagttag aggacaccta tctgatattg gatttttcta
    10621 tacaattact caatatggtg taggtatact tatatttgat attctgttta ttataaaagg
    10681 gttcaaaaca gcattacttg catcaaaagt gggtatgact ggctatcaat atttagcatt
    10741 tggaattaca ttaagtgttt tgctaacagg gattaatata gactggtttt atccgatatt
    10801 tactccagct attcctgtat gcttagcggt tatagaatac atctatcaag aatgtagaag
    10861 tgtggctaac atagaatagg aaatattaat aggagtgcat ctcatgaata agaaagagat
    10921 tttatataac atattgaaga agtacgaaaa tttaccatat gtgtttagga acatgtttgg
    10981 tcataatata agtaaactcg tatcttggaa attgactgct gattgtgcaa cagtagaaga
    11041 aaattctgaa gtttatgcag cgtttcaatt gcgctcaaag gacatgtctg atgttccagt
    11101 gatgggttta gttaattgtt gcaaagatgt agcaattgta atgcaggggc cactatgttt
    11161 aaaggatgat ttctcttata atactctggc ctactataag aaatgttatc caaatgcttt
    11221 aattattctt tcgacatgga aggacgaaga tattaaggca ttgaaaagga tagaggatct
    11281 tggagttata gttatttgta gtgagaaacc tgaaaagcct ggacacttaa atattaatta
    11341 tcaagttgga aacactttgc ctggaatagt cagagcaaag cagcttggtg caaagtatgt
    11401 ttgtaaaact agaacagatc aaagaatata ccatccaaat gcaatggcgt ttttctgcag
    11461 tttacttgaa cagtatcctg ttaataacga agatgattgg aaattaatac aaaatcagcg
    11521 attactgtta ctatcaatgc cgtatggaga catgtttttc ccgtattgtt tgtcagactt
    11581 cctgtatttt ggtgatgttg atgatatgat aaacctcttc tcgattccta gtgatgtgag
    11641 agaaaaggga gctgtgtcta gaggagtctc aagaagggag atatctgaaa ataatttagc
    11701 tccagaagtt cagcttcttc gaagctatat ttcacgaatg gggggcaatg aggagtgctc
    11761 tattagggca tattgggagt ttgtaaaaaa ccatgttatt actattaaca aaaatcaaat
    11821 tgatttgtac tggcataaat atgtaggtcg ttattcaaat aatactattt atggtacgta
    11881 ttatattgat gatagccaag atgcacttca ctgctataat tttgatttta taaattggtt
    11941 gaatttgtac actggaaaat acgaatacaa agcaaaatat gaaaaatata tggactttgt
    12001 ttgggaagag taattaactt ttatagtaaa aagtacttta gggaagggca tattat
//
LOCUS       DACEMO010000076        12025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEMO010000076 DACEMO010000000
VERSION     DACEMO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.078"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            139..459
                     /locus_tag="IW728_001552"
     CDS             139..459
                     /locus_tag="IW728_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024917.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            911..1111
                     /locus_tag="IW728_001553"
     CDS             911..1111
                     /locus_tag="IW728_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1024918.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1258..4047
                     /locus_tag="IW728_001554"
     CDS             1258..4047
                     /locus_tag="IW728_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1024919.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4094..4621
                     /locus_tag="IW728_001555"
     CDS             4094..4621
                     /locus_tag="IW728_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1024920.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4642..5832
                     /locus_tag="IW728_001556"
     CDS             4642..5832
                     /locus_tag="IW728_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1024921.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5872..7170
                     /gene="asnS"
                     /locus_tag="IW728_001557"
     CDS             5872..7170
                     /gene="asnS"
                     /locus_tag="IW728_001557"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1024922.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7965..8639
                     /locus_tag="IW728_001558"
     CDS             7965..8639
                     /locus_tag="IW728_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1024923.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8636..8977
                     /locus_tag="IW728_001559"
     CDS             8636..8977
                     /locus_tag="IW728_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024924.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9007..9366
                     /locus_tag="IW728_001560"
     CDS             9007..9366
                     /locus_tag="IW728_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1024925.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9560..9748
                     /locus_tag="IW728_001561"
                     /pseudo
     CDS             9560..9748
                     /locus_tag="IW728_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            9890..11974
                     /gene="cadA"
                     /locus_tag="IW728_001562"
     CDS             9890..11974
                     /gene="cadA"
                     /locus_tag="IW728_001562"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1024926.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         4006 a         2111 c         2591 g         3317 t
ORIGIN      
        1 aaaagaggtt gtgacgtggt cacgacctct ttttgacgat tatgatacaa aaaacatcat
       61 ttatgaacaa aatgtgaata acgggcgttc ttgagatatg attttaattg ggaaaaactt
      121 atcgataagg aggtctttat gaaaaaaaga ctgttatggt gtttgctgtt tctatcccta
      181 agtttaacaa gtcagccagt ttttgctgaa gaaacacagc ggtctcctat tacaggcaca
      241 cgtctgtaca aaaatgttta tgtacagaca acagatcttg attctgcgat tcctgaaaca
      301 gaagaacggt ctttgcagcc aagatttggt tacttttggt actgtccgac ctgtgattat
      361 gaaagttgct ggcatttctt acattctaca gctgcagctt ttgcttttac tcatactcag
      421 aaatatgatg gacatcatcc tgttatttat gaggtataga tatgagcaaa aaagaaaaac
      481 taatctcgtt gatcgtattg gtttgccttg ttcttctggt cactgtcata ggctatcaga
      541 aataccatga gaacatcaga cctactaaag aatcttcccc agcctcgacc gttgaaacag
      601 aaccgataag tagtcagaaa gaataatttt tttagtttaa agaagaacat cagagcaaaa
      661 aaacaccaac tgaacaatat gtcgtttatt ttaaaaaaag ttcgtttttt cactttatgt
      721 aatcgctgag aaaacttttt ttaataaaaa aagtcgaaaa actctagctt ttttgtaaac
      781 ggtatgatat actataggag tagtttttgt tcggtaatct gtggtagctt gtttcaaata
      841 aacatcttcc agatatacac cattcaagta attgcaaaat atgtgttgat acacaaggag
      901 gatttttatt atggaaacag gtacagtaaa atggtttaac tcagaaaaag gatttggatt
      961 catcactgca gaaaacggaa acgatgtatt cgttcatttc tcagctatcc aaggcgacgg
     1021 attcaaaact ttagaagaag gccaagcagt aactttcgat gttgaagaag gccaacgcgg
     1081 acctcaagca acaaacgtta ctaaagctta attaacctat actaaaaaat aaacgcccag
     1141 acatttgcgt gtctgggcgt ttttgtctat tttctttaaa aagcgatgag agtatttttc
     1201 tgcactttgt agtataattt caaaagaaca ttagaggcag gagaagagga agatattttg
     1261 aaaccaagaa cttatgcagt cgttgatatc gaaacgactg ggacgaatcc aaaagaagat
     1321 cggatcatcc aatttggctg cgtcatgatt gaatcaggtc ggatcacttc acgtttttcg
     1381 atcgatattc atccagggcg gaagatttca aaacaaatcc agcatttaac agggatcacg
     1441 aatcaacgcg tccaaaaagc gccttatttc gaagatgtcg cccaaacgat ttacaatctt
     1501 ttggcagaca ccatatttgt cgcgcataat atttattttg attataattt tttgaaccat
     1561 gaactcatgc gatgcggact tcctagtttg aagataccag gcatcgatac agtagaactt
     1621 gcacaaattt tcttgccgac ggaaccaagc tttcgcttag ctgatttgtc agaaagcctt
     1681 ggatttattc atgagaatcc tcatcaggca gatagtgatg ccgaagtgac aggtcaactt
     1741 cttttgctga tcgaagaacg gatgagaaaa ttgccaatca tcacgttaga gcagattgcg
     1801 cgattaagta gacacacagg tatggatacg agtcgtttca tttatcatgt gattgaagaa
     1861 atgaaagaaa aaccagagcc acttgattcc tcattagaaa tcgtcgatgg gctagctttg
     1921 cagaaaaaag aagtggaact attcacatcc gtccattacg gcgagcggac atatccaaga
     1981 aaaaaacaag caaaagaaaa gatgtttggt aaaacactga tgtatcgaaa agaacagaac
     2041 agactgatga atgcgatcta cgaccatttc acaaaagatg aatcaaaaga tttgatgatc
     2101 gaagcagcta caggaatggg taaaacaatc ggttatctgt tgccattgag ttatttggct
     2161 acacctgaaa aacccgctgt catcagtacc gtttcgctag ttttgcagca gcagatcata
     2221 gaaaaagaaa ttccattgct gaatcaattg ctagatcagc ccatacaacc tgtaattatc
     2281 aaaagttatc gccactatat cgacctgcaa cgatttaaag gaacacttgt ggaaccacca
     2341 gaacaaaaac aatatgcttt gtatcaaatg gcggtcttag tttggctgac gcaaacgaag
     2401 accggtgatt tggatgaatt acatttaaca aacttgaacc atgcattctt tgcagatatc
     2461 gcgcacagag gtactgggtt tttagcaaga aatcagtcgt tttacgaaca agactttgtt
     2521 cgtttcttgc aaaagaaaat caggcaaagt aattttttga ttgtcaatca tgcgttttta
     2581 gtacaagaaa cacagcgaaa agaaccattg cttccagcta gtccattttt gttgatcgat
     2641 gaagtacatc atttgccgga aatcgctgaa aaagtaaatg acaaacgatt aagtattaac
     2701 tatttccata aacaaatcca gcaattagaa gaaccaaatc agctgtttga ccgaatacaa
     2761 agctatttac cgaaagaaca tgaagcctta cgctggttat cgatttatcg agaagaactt
     2821 tctgcgctat gcgaagcaca gatttgtatg gcagaaggat taatgagttt agtgagcggc
     2881 aagcaaccca acggacagta tccagaagaa gtcatgatga caaaagagat gctggatcag
     2941 ctgccactgg aatctgggac agccattgat cacctgcagc ttttttatca ggagattcag
     3001 cagctacagt cacaaatgcg tctgactttt gagattatcc aagaaagctg gacaaaccag
     3061 atgcggatcg atttagcaag tttatatagc ttatttgatc aattagacaa gcagtacttg
     3121 ctgatggatc aatggctgga agactggcga gatcatttga tccattggtt cgttcctggg
     3181 aacacagcgc agcagatgat ttttcatctc catgatttcc aagcggctat gttgccgtcg
     3241 actgtttggt atcctcgata tgatcgtatt ctttatattg gaggaacgct gaagatcggc
     3301 cctaatcgac actatctctc taagaggctt ggcattgaag cagcacctct taaagtgatt
     3361 gcctctcctt atgattatga gaaacaggca aggatctatg tgccaaaaga ggcacctgcc
     3421 gtccatcaag tcagcaatca agcatacatc gattatttag agacagcact tacaggtctg
     3481 atcgaacaag aagatcggcc aatacttgtg ttattcactt ctcatgatgt tttgcagaag
     3541 gtctatcaga aaatgcatct gtctatgcta gaaaaaggac gagaaatact agctcaaggg
     3601 tttggtggaa gccgggagaa gcttttgaaa cgcttcattt tgtcagaaaa aagcgtattg
     3661 tttggcgctg acagcttttg ggaaggtgta gatttgcctg gagatgcttt gcagatcgtt
     3721 gtagtcacac gtttaccctt cgataatccg cttcgcccga tggtcaaagc taggaacgct
     3781 tatttagaat cagaggggat caatcctttt taccaggaat ccgtacctaa agcagctttg
     3841 aagttacgcc aagcattagg tcgcttgata aggacggaga cagatcgtgg cgtattgctt
     3901 attttggatc gaagactagt cactgcaaaa tatggcgcac ggatcatcca tgcattaccg
     3961 aaaaaagtac cgatcaaaga gctagctatg gaggaaatct atcaggatat ccatgaattc
     4021 ttaaagaaaa atgaagaagg aagttagagg agctgtcttt tttcataatt aaaatctgct
     4081 ataatgaaaa ggaatgaaga taaaggagcg acgacccttg aagaaaaaac agacaaagga
     4141 aaaaacgatt aatcgcgtgt tacttggctt gatcgtattt ttattagcgg tcatcgtgat
     4201 cagctcgatt ttttacttgc gttccagtcg tccggcggtg caggcgaaga aagaagcagt
     4261 agaaatcgca aagaaatatg caaatcttga ggaaaccgat catttttatt ggtttactcg
     4321 aaagaagaca tacttttcac tgacaggtac aaatgataaa ggaaaagaaa tcgccgttat
     4381 cattcctaaa tctggtgaaa aagtaaaagt attggatcaa acaaagggac tgactgaaaa
     4441 cgaagcaaaa cagacaattg ctgcggccca tcctgaaatc caaatcgaaa aagctgcttt
     4501 agggatgtat gatgatcagc ctgtgtggga agtagtagga aaagaaacaa atgggaaact
     4561 gaattattac ctgattggct ttgaatctgg agatgaagtc aagacaatca agggaattta
     4621 aaaggtaaag gaatgaatgg gatggagtta tcaaaaagaa caaaacgatt agaaccgtct
     4681 gtcactttgg cagctgcggc aaaggccaag gcattaaaag caaaaggcag agatgtgctg
     4741 agtttgactg tgggagaacc ggattttgcg acaccagaaa acatccaaga agcagcaatc
     4801 gaagcaatcc gcaatgggaa agctagctat tatacaccaa cagcaggaat cccagagctt
     4861 cgacaggcaa tcgttgatta tctgaagaaa aactatggac ttgtctatga accttcacaa
     4921 acagtcgtaa cagacggagc gaaatttgct ttgtataccc ttttccaaac gatccttgac
     4981 ccgcaagatg aagtcatcat tcctgtgcct tactgggtca gttacggtga gcaagtaaaa
     5041 ctggcagaag gacttcctgt ctttgtgaaa ggggaagaat ccaatagctg gaaagttact
     5101 gttgaacagc tagaacaagc acgttcagaa aaaacaaaag ccgtaatcat caattctcca
     5161 tcgaatccga ctggaatgat ttatagcaag aatgaacttc aggcaatcgg tgaatgggca
     5221 gtaaaaaatg atattttgat agttgctgat gatatttatg gtcgtttagt ttataatggc
     5281 aatgagttta ctccaattgc aacgatttca gaagcaatca aaaatcagac catcatcatc
     5341 aatggcgtgt ctaaaacata tgcgatgaca ggctggcgca ttggttatgc ggtggggaat
     5401 tctgagatca tcaatggaat gattgctgtt gcttcccagt caaccagtaa tccgacagca
     5461 gtcagtcaat atgcagctgt tgaagcattg aaaggtgagc aagatacagt agaagaaatg
     5521 cggaacgcct ttgaagaacg cctgaatacg ttatatccgc ttgtggcaga actgccaggt
     5581 gtttctcttg aaaagccgca aggagctttc tatctttttc ctaatgtaaa agaaacgcta
     5641 cgcatgtgta aatatgaaaa tgtcacacaa tgggtagaag atcttttaga agaaacaggg
     5701 gtagcgcttg tgactggcga aggctttggg gcaccagaaa atgtccgcat gagttatgct
     5761 actgatcgcg ctacgttaga agaagcggta cgcagaatca agcaatttat tgagagtaaa
     5821 agtcaaaatt aatcctgaag aaattcaagg aatataaaag gagagtcata cgtggaacaa
     5881 attcaaatca tcgattcgaa aaaccatgtg ggagaaacag taacaatcgg cgcttgggtc
     5941 gcaaataagc gatcaagcgg aaaaatcaca tttttacaat taagagacgg aacagccttt
     6001 ttccaaggtg tagttgtaaa aagcgaagtg tcggaagaag ttttccaaac agctaaaaat
     6061 ctacaccaag aaacatccgt gttagtaact ggagaaatcc gtgaagacag tcgttcaaaa
     6121 tttggttatg aaatcggaat tacaaatgtg gaagtggtaa gcgagagcca cgattatccg
     6181 atcacaccaa aagaacacgg aacagacttc ttgatggatc atcgtcattt atggctgcgt
     6241 tcttcaagac aacatgcgat tatgcagatc agaaatgaaa tcattcgtgc aacgtatgaa
     6301 ttctttaatc aacatcattt tgtcaaaatt gatccgccga tcttgactgg ttctgcacca
     6361 gaaggcacta cggatctatt cgaaacaaat tactttgatc aaaaagctta tttatctcaa
     6421 agtggccaat tatacatgga agctgctgct atggctttcg gaaaagtctt ttcattcggt
     6481 ccgactttcc gtgctgaaaa atcaaaaaca cgacgtcatt tgatcgaatt ctggatgatc
     6541 gaaccagaaa tggctttcac tcatcaagaa gaaagtttag aagtacagga acaatatgta
     6601 gcattcttag tacaaaatgt tttagatcat tgcgaatacg cattagatgt actaggacga
     6661 gacaaagaag tcttaaaaac ttatacaaaa ttgccattcc ctcgtattac ttacgatgat
     6721 gcaatcgaat tactgaagaa aaatggattt gatgacatcg aatggggcga agactttggt
     6781 tcacctcatg aaacgttcat tgcgaattca ttcgaacagc ctgtctttat cctaaactat
     6841 ccaaaagcca tcaagccatt ctacatgaaa ccacatccaa caagagaaga cgtggtaatt
     6901 tgtgcagata tgatcgcacc agaaggttac ggtgaaatta ttggcggaag cgaacgggca
     6961 acggactatg atttcttgct agaacaaatc cgtaatcacg gtttggatga aaaagaatat
     7021 tcatggtatc ttgatctacg taaatacggt tctgttccgc actctggttt tggtctaggt
     7081 cttgaacgaa cagtcacttg gattgctggt atcgaccatg tacgtgaagc tattccattc
     7141 ccacgtttgt tgaaccgaat ttatccataa gaggttgtgc gcctgccgtt cagctccgag
     7201 tcacaaggag taatttcaaa aaacagtttt tcatgtttga tgactattgt gacttgtggc
     7261 cggagagcaa cgtttattta aaaaaaagaa ctgcgaactc tcgcagttct tttttttaaa
     7321 tacgggataa tggaagtttc cagcagacaa gagaagaaag caaaagaaat tgctatttga
     7381 ttcacaaggt tctataacga aatcgcaatc aatcaaacat ttacaaagaa ggtaattcaa
     7441 atggaaatct gatttgatga tgcttctgct gttgaaataa agcagcatct agcacctgaa
     7501 aacaaactaa tagtaacatt cgaagatgga gtaggtccgt attttcagca tgcaataatc
     7561 catatgtaag ttcaattcac ccttgaaatc atcggaccag ataaagttat gaccaatcta
     7621 tcccaagcaa tctagaagag atattagtaa agggacattt actagaagtc ttagatgagc
     7681 ctataaatat atgtttcgat ggcgtcatca gtggtctgat tgacggaagc ttgggtgtga
     7741 tcgatctctc tggaccaaac aacgaaggaa tcaaggggaa tccggcacgt taagaaaaaa
     7801 atagcataaa aaactagaca cttgatgagc gttcattcaa ttgtttgaag gaactaggct
     7861 ggtgtcattt tttatacata tattttctct ttcgtcttga tagggcttat gggataatat
     7921 aaggaagaaa atcatcgttt tattcaaaaa ggagtttttg acaaatgaaa aaacgtatat
     7981 tatttatcgg tgatagcatt acagacgcag gaagataccg agcagaagca actgatctag
     8041 gtaaaggtta tccacttttg attgccggaa aactaatctc ccaatatcca acgatggaat
     8101 ttgaatgcat gaaccgtggg ataggaggag acaaaattgc agatctgcaa gtacgctggc
     8161 aagaagattg tttagcttta caacctgact atgtatcgat tttgatcgga atcaatgata
     8221 cctggcataa tactagcgat caggctatat tcggaactga gaaatctgct cagcaatttg
     8281 aatccgttta tcgaaaatta ctaaatgaac ttcaaacagc ggaaatctgc aacatcatct
     8341 tgatggagcc gtttgtactt cctataaccg acgaccgtca tggctggcgt aaagacttag
     8401 accctaaaat ccaaattgtt cgtaaacttg ctaaggaata tggcgcattg ctggttcctt
     8461 tagatggtat attcaatgct ttagggattg cttatggttt ttcgaaatat acaagagaag
     8521 atggggtcca cccaacgatt ggcggacatg agatcattgc acaaagctgg atagatgcaa
     8581 ttgtcgcagt cgattttttt agtggagata aaaatgagcg aaagaaaagt gaaaaatgat
     8641 ggaaataagg tgggcagtaa aagaagattt acctcaaatc aatcagctga ttactgatca
     8701 tccagaatca ttcgtaaaag caaaaacggc agctgaccta aatgataaga ataaactatt
     8761 gctcgttgct gtaacaaaag aaaaacggat tatcggattt gcgcaagcgg acgtgcaaca
     8821 gtcagaagaa cgattggatc agcgaccatc aggaaaacta atccaacaca cattagtaac
     8881 aaaagaaaat acggaagaaa tccaaaaaaa atttgcagaa tacttgatgc tggaagctaa
     8941 aaaaagaaga ataaaatcaa tttttgccca gttttaatca aaaacagtaa tagaaggaga
     9001 aatacaatga gttgtttagg aaatgtcatc tggtttatat tcggcggact aatcggtggt
     9061 cttagctggt tagcagcggg gatactttgg tgtatcacga tcataggtat cccaatcggt
     9121 atgcaatgtt ttaagatcgc ctccctttct tttgcgcctt ttggcaaaga agtaatttat
     9181 caccagtctg gcatgtcatt gatcgttaat atcatctggc tgatcgtttc aggcctacca
     9241 attgctctcg cacatgtcgt cagcggcatc cttctatgta tcacgatcat agggatacct
     9301 tttgccaagc aatctttcaa acttgctcga ttggcgttga tgccattcgg tgcagaaatt
     9361 gtataaaaga gaataataaa ggttagataa agcatagagc caggtgggat acatgtattt
     9421 cacctgactc tatgcgtttt cctgtataag ttcttctatg tgacttttac gtcttttatt
     9481 tatatggggt tactgtttat ggaaagttga aataattttc attaaagaac tctttctata
     9541 tttttgaaag cgtagtggag cttcgtatat ctgggaagga cattttgcag ggattgaaaa
     9601 aagatattgt cctatcgaaa aattaatgaa tttagacaaa ttgccagcaa caggcttcaa
     9661 acttagttgc tatcctgtca agatcaagaa ggcaagtgcg ggttggataa gagcagtggc
     9721 aatgatcgaa gagaaaaaga aagaataagc caaaacatct gtatggaaga aattttccga
     9781 tgcagatgtt tttcattttt tctttcggat accttgacat atgaataacc attcatttat
     9841 aattagttat gaagagatat tcatatgttg aggcgaggag gtcattaaaa tgaaaagtta
     9901 cagattagaa ggtttagatt gtgcgaactg tgcaatgaaa atcgaaaaag gagttcagaa
     9961 aatcaatgga gtcaaggaag caacggtaaa cttcacttct ggaaaactaa cgattgacgc
    10021 agaggaagat catttggcta cgattgaaca agaaacaaaa aaagtagtga aggaattaga
    10081 accagatgtc aaagtaacag aaatagataa ggaaaaagta tctgaacatg gaaatgaaaa
    10141 aaagagaaat acgctgttcc ggattttatt ttcccttgca ggtatcgctt tattgctatt
    10201 atttgatttt aatgaaccaa ttcgcttgat agggtattta ttgatttatc tgctgatagg
    10261 atatgatgta gtgaagaagg cagtgatgaa tatcgtcaaa ggaaaaatct ttgatgagaa
    10321 ttttcttatg tctgttgcta cgattggcgc tatgttgatc ggtgaatatc cagaagccgt
    10381 agcagtgatg ctattttatc aaatcggcga gtatttccaa ggattggcag tcagtcattc
    10441 aagaaaatcg attcgcgaat tgatggcgat ccgtccagaa acagctcacg tgcagacggc
    10501 ggaaggattg atgaccgtga accctgaaga cgtgctgatc ggtcagtttg tacttgtgaa
    10561 gcctggtgaa cgtgtaccgt tagatggaac gatcatcgaa ggagaatcat tagtcgatac
    10621 gtcagcactt acaggagaat cagtccctaa atctgtatat gttggtgaaa cagtattaag
    10681 cggatttatc aataaaaata aaccattttt agttcaagta gaaaaatctt atgaaaattc
    10741 tactatcagc aaattattag aattagtaga aaatgccagc agtaaaaaag caccagctga
    10801 aaatttcatt actaaattcg cgcgctatta cacaccagtc gttgtaggat tggccgtttt
    10861 attagctgtt ttacctccac ttgttgtttc tagagcggcg ttttctgaat ggatctatcg
    10921 agcattgaca tttttagtga tctcatgtcc atgtgcatta gtgatttccg tgccacttag
    10981 tttctttggc ggtatcggag gagcaagtaa aatcggtgta ttagtaaaag gttcaaatta
    11041 cttggagttg cttgcacaaa ctgagacagt ggtattcgat aaaactggca cattaacgaa
    11101 aggtgacttt tcgatccaga cgatcgatcc aacaggaatt gatccaaaga aatttatgca
    11161 atatgttgca agtgcagaac agttttctac acatccgatt gctcaatctg ttttagaagg
    11221 ctacgcaggt ccgcttttac caacagctaa tatccaggaa tttgcaggtg aagggatctt
    11281 agctgaagtt gatggcaaac aagtattagt aggtaatcac aaattgatgg aaagatttga
    11341 aatctctttt ccttcaagtc aagaaatcgg tacattgctt tatctagcga tcgatcaaag
    11401 ttacagtggt tatcttgtta ttgcagatac cttgaaagaa gatgccgtag acgcattggt
    11461 tcaattaaaa caggctggtg tgaaaaacac agtcatgttg acgggcgact cgaaaaaaat
    11521 tgctgatcat atcgggaagc aagtaggcgt ggacaagata tatagcgaat tgcttccaga
    11581 agataaagtg cagcggttag aggaaatctt acaaagtaac aataaaaaga ccgcatttgt
    11641 cggagatgga atcaacgatg caccggtgct agcaagagca gatgtaggaa tcgcaatggg
    11701 tggattggga agcgatgctg caattgaagc agcagatgta gtcatcatga atgatcaacc
    11761 atcgaagatt gcagaagcaa tccatttagc aaagaaaaca ttgaaaatcg tcaaacagaa
    11821 cattgtcttt gctattggga taaaaatttt ggtactttcc ttaggtgctt ttggttttgc
    11881 atcaatggga gatgcagtat ttgcagatgt tggggtgaca gtattagctg ttttgaatgc
    11941 catgcgaagc ttgcatgtaa aaaataaacg ttgatcaatg aagaggacga atgaacgtcc
    12001 tctttttttt atatcctttt cttaa
//
LOCUS       DACEMO010000077        11885 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEMO010000077 DACEMO010000000
VERSION     DACEMO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.079"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..2714)
                     /locus_tag="IW728_001563"
     CDS             complement(3..2714)
                     /locus_tag="IW728_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1024927.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(2724..3425)
                     /locus_tag="IW728_001564"
     CDS             complement(2724..3425)
                     /locus_tag="IW728_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024928.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            3611..4210
                     /locus_tag="IW728_001565"
     CDS             3611..4210
                     /locus_tag="IW728_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1024929.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4291..5304
                     /locus_tag="IW728_001566"
     CDS             4291..5304
                     /locus_tag="IW728_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1024930.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            5412..6329
                     /gene="rbsK"
                     /locus_tag="IW728_001567"
     CDS             5412..6329
                     /gene="rbsK"
                     /locus_tag="IW728_001567"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1024931.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            6357..6752
                     /gene="rbsD"
                     /locus_tag="IW728_001568"
     CDS             6357..6752
                     /gene="rbsD"
                     /locus_tag="IW728_001568"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1024932.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            6752..8125
                     /locus_tag="IW728_001569"
     CDS             6752..8125
                     /locus_tag="IW728_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1024933.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            8482..10626
                     /locus_tag="IW728_001570"
     CDS             8482..10626
                     /locus_tag="IW728_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1024934.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(10678..11718)
                     /locus_tag="IW728_001571"
     CDS             complement(10678..11718)
                     /locus_tag="IW728_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1024935.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         3629 a         2402 c         2253 g         3601 t
ORIGIN      
        1 ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt gaaatgggta
       61 acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac ataagcccac
      121 catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa gatgaagtca
      181 gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa gatgatattt
      241 tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt tattgtagat
      301 aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac gataaaagcc
      361 agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc cattgattct
      421 tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc tgtggcaagc
      481 agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg cagcaaaaat
      541 gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga catataaata
      601 aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc tttttgatct
      661 tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc tactttttgc
      721 gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc agttaaatgg
      781 ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact ttgcgaagca
      841 tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc atttactttt
      901 tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga tgaatccaat
      961 gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac agaacttaga
     1021 gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat tccgataatc
     1081 gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt ttgattaaag
     1141 ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc tggcttcggt
     1201 gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa aacgatcatt
     1261 gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg cggccagtca
     1321 taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa aatgatcctt
     1381 ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga ggctagactt
     1441 gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc ctttaatgtc
     1501 ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt gatcaatgcg
     1561 gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg atcagacaga
     1621 tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta tgatggttta
     1681 gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga ggccagttca
     1741 cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg ttgagataat
     1801 gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc tgactgttgc
     1861 gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg tgctttgcca
     1921 tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt cagctcttct
     1981 aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt ttgctccatt
     2041 ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa atcaagatat
     2101 aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa ataatcgacg
     2161 ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa tggagaacgg
     2221 acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat ctgataaata
     2281 tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg gtttggtttt
     2341 tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt gttataagaa
     2401 aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt tcctgtttct
     2461 acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa gccaccagta
     2521 gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat cgttttttgc
     2581 atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat gacaataagg
     2641 atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc tttaagatat
     2701 gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga aggatgatca
     2761 tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc agcgatgggc
     2821 gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct gctagcgttt
     2881 tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt cggttcatca
     2941 cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct ctgctgttcg
     3001 cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac ttgcctcaat
     3061 acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac attttctttt
     3121 gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc tgttcgtcgg
     3181 taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat aatgatctga
     3241 ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt tcctgctcca
     3301 cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat atcgtcattt
     3361 gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac ttcgacatac
     3421 ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat cgaatgcgtt
     3481 ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg aatcctttaa
     3541 tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt tatttgaaag
     3601 aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa aaactgcttt
     3661 tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc cacacactgc
     3721 agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta ccgctgcctt
     3781 gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac aaactgatat
     3841 gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga aggattcttt
     3901 gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt tttttggtac
     3961 ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa gcggtgtagc
     4021 gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt accatatcgt
     4081 gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt tcataaaaaa
     4141 gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag ctatccaaca
     4201 aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt catgggaaaa
     4261 taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac aatcaaagaa
     4321 gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa tggaaatacg
     4381 gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga attaggctat
     4441 cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat tggtgtactc
     4501 gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga ggatatcttg
     4561 tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca aaaagaaatc
     4621 gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc gacttctgct
     4681 gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt cattgttttg
     4741 gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt tcgcggtggt
     4801 tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct tgtctatcct
     4861 gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc tcttgatgtt
     4921 taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa acaaggtggc
     4981 taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc tttaaatgat
     5041 gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat ccctgaagac
     5101 tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc gaaactgaca
     5161 acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt gttagatcgg
     5221 atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt agaaaaaaga
     5281 ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc gaactattaa
     5341 aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg agatagttgg
     5401 aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac actacccttc
     5461 gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt tttcacgctg
     5521 gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca cggacatctt
     5581 ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta ctgacaaaag
     5641 aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga caagcaatga
     5701 ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca aataatgctt
     5761 tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac tttattattg
     5821 ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca aaagacgcag
     5881 gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact gaactacttg
     5941 aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca ggaatcagag
     6001 tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta ggtattggaa
     6061 cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa gaacacggta
     6121 tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat acatttattg
     6181 gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg atcctttatg
     6241 gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct atcccttacc
     6301 gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa acaaacatga
     6361 aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg gggcactttg
     6421 ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa aaaatagatt
     6481 tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt ttagaagaac
     6541 ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg gaaatattgg
     6601 aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat gaagaaatga
     6661 aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca ccttacgcca
     6721 atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc ctgaacagat
     6781 ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag acaatattga
     6841 ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc cagtagccaa
     6901 tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac tcgatagata
     6961 tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga cgcgtcaaat
     7021 cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg gtttgatatc
     7081 ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa aaggtctggt
     7141 tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc atattttgtc
     7201 tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt ctgctgccaa
     7261 aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga ttgaaccgaa
     7321 tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg ggattcctgt
     7381 agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt tcttttattc
     7441 ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag tatttgtacc
     7501 tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc ctgcgggtgt
     7561 ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta acggaccgat
     7621 cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta tccaatgggc
     7681 tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa tcgttccgtt
     7741 gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc tgcgaagtaa
     7801 acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac ttgcgggagt
     7861 gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg ttgctgcttg
     7921 cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag cgatcgcttt
     7981 tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt tctggtactt
     8041 agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta gcgggtttga
     8101 tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag atgtttatca
     8161 catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt gatactttcc
     8221 aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt tatccccaaa
     8281 caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg ctcgttgctt
     8341 atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa aagcttttcg
     8401 acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga aaatcaatga
     8461 aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct agctgctact
     8521 tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga taatcaaata
     8581 gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag tgaaacaacg
     8641 gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc gaccgagcca
     8701 actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac atattccctt
     8761 acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc ggattcccct
     8821 gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt agcggaaacg
     8881 aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt cgctgttaat
     8941 ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa caatttatct
     9001 attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac tgatcctagt
     9061 gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt gggtaataac
     9121 aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa cagcgggaat
     9181 tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca gagagaacca
     9241 aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc tcgtccaaat
     9301 acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt tgttgtaaaa
     9361 tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc aatcgttcga
     9421 gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc aaatactttt
     9481 gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta tatcggtggc
     9541 tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc ccaacctggc
     9601 gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat ctatattggg
     9661 aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac ttctatttca
     9721 gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc tgcttcaggt
     9781 tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca tttcagtcaa
     9841 aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga aatcgggcaa
     9901 aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag tggatctgga
     9961 aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg atcatataaa
    10021 ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag ctaccaaatc
    10081 cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta tgccgacttg
    10141 atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa gtcggaaaca
    10201 aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga tcgccaatat
    10261 tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga tgaaccgaaa
    10321 aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc taagcgattc
    10381 gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga attttcaacg
    10441 ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg ggatttgtgg
    10501 aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa agctgcaact
    10561 gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct gcctttaaag
    10621 aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta atttattcta
    10681 tttctttata tttattttgt attccgttac aaactcgcgt acttggttag gttccagagt
    10741 gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct caaatgtgat
    10801 accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc caaaatttgc
    10861 tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt tttcatctgg
    10921 gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa atggatgatc
    10981 gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac ctaatttttt
    11041 aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg taggcgttgt
    11101 atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt cgtcgattga
    11161 ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga atactgtttt
    11221 gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca gcgtataccg
    11281 tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag tctgatagaa
    11341 aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct tggcttcaaa
    11401 gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta cttcgaacgt
    11461 ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc cgattgaagc
    11521 accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta agatcaactc
    11581 tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa gatcactgac
    11641 agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt cgccaaaatt
    11701 ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac tgattccagt
    11761 atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa aatttactat
    11821 attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca acctcttttt
    11881 ccgaa
//
LOCUS       DACEMO010000078        11800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEMO010000078 DACEMO010000000
VERSION     DACEMO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.080"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..966)
                     /gene="hflX"
                     /locus_tag="IW728_001572"
     CDS             complement(<1..966)
                     /gene="hflX"
                     /locus_tag="IW728_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1024936.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAM"
     gene            complement(968..1885)
                     /gene="miaA"
                     /locus_tag="IW728_001573"
     CDS             complement(968..1885)
                     /gene="miaA"
                     /locus_tag="IW728_001573"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1024937.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(1889..2626)
                     /locus_tag="IW728_001574"
     CDS             complement(1889..2626)
                     /locus_tag="IW728_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1024938.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2717..3244)
                     /locus_tag="IW728_001575"
     CDS             complement(2717..3244)
                     /locus_tag="IW728_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1024939.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3424..4017
                     /gene="clpP"
                     /locus_tag="IW728_001576"
     CDS             3424..4017
                     /gene="clpP"
                     /locus_tag="IW728_001576"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1024940.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4121..4606)
                     /locus_tag="IW728_001577"
     CDS             complement(4121..4606)
                     /locus_tag="IW728_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1024941.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(4759..4956)
                     /locus_tag="IW728_001578"
     CDS             complement(4759..4956)
                     /locus_tag="IW728_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024942.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5051..6811)
                     /gene="efrB"
                     /locus_tag="IW728_001579"
     CDS             complement(5051..6811)
                     /gene="efrB"
                     /locus_tag="IW728_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1024943.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(6808..8538)
                     /gene="efrA"
                     /locus_tag="IW728_001580"
     CDS             complement(6808..8538)
                     /gene="efrA"
                     /locus_tag="IW728_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1024944.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            8931..9143
                     /locus_tag="IW728_001581"
     CDS             8931..9143
                     /locus_tag="IW728_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1024945.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9145..10824
                     /locus_tag="IW728_001582"
     CDS             9145..10824
                     /locus_tag="IW728_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1024946.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11078..11278)
                     /locus_tag="IW728_001583"
     CDS             complement(11078..11278)
                     /locus_tag="IW728_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1024947.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11428..11589)
                     /locus_tag="IW728_001584"
                     /pseudo
     CDS             complement(11428..11589)
                     /locus_tag="IW728_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3441 a         2547 c         2050 g         3762 t
ORIGIN      
        1 catcgctggg tcgatctggt ctgccttatt gtaaacagtc agtatcggca tttctttcat
       61 attcagttca tccatcaatt ttaagacagt ctgctcctgt aaaatacgat ctggagaact
      121 agcatcgact acatgtagca aaagatccat attttgactt tcttctaatg tagaatggaa
      181 agcatcgatc agttgcgtag gcaggtcttg gatgaacccg acggtatccg ttacagtaat
      241 ctcaaatcct tcagcaaaac gccatctctt agtcaatgga tctaacgttg caaacaattg
      301 atctttcgaa taagtatccg cttgtgtcaa tagattcaag atagttgact tcccggcatt
      361 cgtgtagcca ataagtccaa tctgaaaaat ctcgctattt tttctttttt ggcgattgcg
      421 ctctcgatga gcttctactt ctttcaattc tttcttaaca gcaaaaattt tattccgtat
      481 atgtcggcgg tctgtttcta actttgtttc acctggccct cttgttccga tcccaccgcc
      541 aagacgagat aatgttttcc cttgccctac taaacgaggc agcaggtatt ctaactgagc
      601 aagttcaacc tgcagcttcc cttctttcga acgagcacgc atcgcaaaaa tatctaagat
      661 aagctgcacg cgatcaatta ccggaagacc aacagcatcg gtaatcagtt ggctctgtct
      721 cggagttagc tcatgattaa agatgatcaa atccgcttca tgcgcatccg cttgttgaac
      781 taattcttgt aatttccctt tgccgataat cgtttgccga tccacttgtg gtctcttttg
      841 tgtaattgtg aatacgactt ctcctgatgc ggtattcgtc agctgaacta gctcttccat
      901 tgactctgaa aagtaacgct ggtttgcttc ggtttctacc cctacgatga tcactttttc
      961 ttccatttta ttccgcctcc agccattttt tgattgtttg ctcaatctga cttttttgat
     1021 ttggctcact tataagatca aaccactgtg catccaaacg gttacgaaac cacgtcaact
     1081 gacgtttcgc gtatcttctc gaatgtagtt tgacttgttc tgttgcttct tccaatgttt
     1141 ctttcccttc aaaataaggg aaaaattctt tatatccaat accttgactg gcctggactt
     1201 ctccaagctc atacacctta cgcgcttctg ctactaaacc ttctgctagc atcaaatcga
     1261 ctctttgatt gattcgttca taaagaatac tacgcttcgt atctaatcca atcatgaggt
     1321 aatcatacaa ttgctttggc tcttctttag gtgccgtgat actatgtcct gtcgtctcta
     1381 aaacttccag agctcttatc actttgcgtt gattgttcca gtggattttc tcagcagctg
     1441 ccgggtcttt ttctttcaac tgattccata atgcttggga accaattgtt tcagctaacg
     1501 tttcatactt ttttctaatc tcaggattcg cttcttctgt cgctcctaac tgataatcat
     1561 atagaaggga ttggatatag agacccgtac caccagctat gataggtagg tgccctcggt
     1621 tcgcaatctc ggtaatcaat tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt
     1681 gagtaggttc gatcacatca atcaaatgat gcggaacatt attcatctct tcttctttga
     1741 tttttgctgt accgatatcc agatttttat agatttgcaa tgaatcacca ctgatgatct
     1801 ctcctccaaa tagttgtgcg agttccacac ttagcgctgt tttaccgact gctgtcggac
     1861 cgacgatcac taatactttt tccatatttt acctactttt cgttgattgt ttccaccgaa
     1921 gagctttctc cggaaaatca gttattattc catctatttt gagttcttga catttggcca
     1981 tttcttcttc atcgttaatc gtccatgcac gaattgcttt tggaaaatca gctgcctgga
     2041 ctggatgttc gaacaaccaa gtattttttg gatgtactcc ttctatataa ggggtttctg
     2101 ctgctagttg gatcttttta tcagaagtac tcatcaataa gtccagctgt atctccggtt
     2161 ccagtaatgc gattttttcc aaagaatcca aatgaaagct gcaatatgca tgttgaaaag
     2221 gccaatctct actttttaat gtttcggcta atttttcttc gataccggga tattcatact
     2281 gatcagtttt aatttcaatc attagcatcc ctcggtaatt tttttggagg agcagtccta
     2341 acacttctct caattcagga actttagctg ctggaaattc tttgctgaat ttgccccctg
     2401 cattcaattt tttgagttct tccaatgttt tttctcgaat cagcccttta ccgttagtcg
     2461 tccggtccac ttcttcgtcg tgcataacga tcaaataacc atctttgctt agatggacat
     2521 ctagttcgat accatctgca cctactcgta cagcttctgc aaatgcagga agcgtatttt
     2581 ctgggtgggt gccgctgctt ccgcgatggg catagatttg taacatcttt cttcactccg
     2641 ttcagttcta ttgtatcatt aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg
     2701 gcaatgcttc aaaatatcag ctccaaacat catctttcga gaagaaacca atcggctggc
     2761 gcgtcatagt cttcaactga gctttttttt cattgatcat ttcagtcaga ctatccgtaa
     2821 aattctctaa aatcacgcgt ccgcctttat aagcatatcc accaatcttc attgtttcat
     2881 ctggagataa aatcactttt cgatgggaga tattcttaaa tagctcgtct aaaatataat
     2941 ttggcagata aaaactattt tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg
     3001 tgatataaca atcatcaaat gcatcaaagc tgacatattc ttccatcagc gttgtccgtt
     3061 tttgcaagat ctcgttgatc tttttattta attgattcag cgtctcgatt tctttgactt
     3121 tcaattccaa ttcacgtcta cgctgttctt tattttccaa gtgacgcatt tgcagtatca
     3181 tgccgatata gacaactaca gcacctgtca gcactccgat aaggccagta ataccaacta
     3241 tcaatctgtt gttcctcctt gttttgtttc tctgcttatc ataccataaa gcgggtttat
     3301 gcgactaatt tgcctaagtt ttttatttga ccttttttga ccaaaaatgt atactacatt
     3361 ttaggtatta gaaactaaat tatggtacac tatttctaaa cgaaatcaag gaggaactct
     3421 attatgaatt taattcctac agtcattgaa caatcttcac gaggtgaaag agcttacgat
     3481 atctactcac gtttattgaa agaccgtatc atcatgttaa gcggccaagt aacagatgat
     3541 cttgctaatt cgatcatcgc ccaattgctt ttcttagacg ctcaagattc tgaaaaagat
     3601 atctatcttt atatcaattc tcctggtggt tcagttacag caggtatggc tatttacgac
     3661 acaatgaact ttgtcaaagc agatgtccaa acaattgtta tgggtatggc tgcttcaatg
     3721 ggaagcttct tgctgacagc tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag
     3781 atcatgatcc atcaacctct tggcggcgct caagggcaag caactgaaat cgaaattgcg
     3841 gcacgccata ttttgcaaac acgcgaacgc ttaaacaaaa ttttagctga acgcactgga
     3901 caaccgcttg aagtgattga aaaagataca gatcgtgaca actatatgac agctgaacaa
     3961 gcaaaagcat atggattgat cgatgaagtc atggaaaaca gttcttcatt aaattaatct
     4021 gtttcaaagg aagctttttt aagctctctt atctaaacca aataaaaaag gatggtcaac
     4081 tcaaactgac catccttttt tattttatgg atgctactgc ctatctttgc tccttgaatt
     4141 ttccttgcgc atgcatccga cctatctcga cataatgctg gatgttttct ttgttttttt
     4201 gtatttcttc ttttgtcaaa ggacggatca ctttagctgg gcgaccaaaa gccaagacat
     4261 taggaggaat aacttttcct tctgttacta atgatccagc accaatcaaa ctattctcac
     4321 caataaccgc atgattcaat atcgtggtcc ccatcccgat cagcgcccct ttttcgattg
     4381 tacagccatg tagcatgcac tgatgtccta ccgtcacatt ctccgcaata tctacaggtg
     4441 catcatgatc gacatggatg attgtaccat cttggatatt cgttctttgt cctattttta
     4501 tccaattact gtccccgcgt aaaacagctt gataccagat cgtcacatct tctgacaatg
     4561 tcacatcacc aatcactgtg gcatttgatg ctataaaccg ttccatattt atttttctcc
     4621 tttcattttt tgtagaaact aatcctattg aataaaggta tcatagcata tatttatttc
     4681 ataagaaaaa aaacaggtaa aacctgctca ggccagatgt ctatttacag tatctggtaa
     4741 ctgacgtttt acctgttttt attgtacatc tatattttct tctattactt tttttaaaat
     4801 taattcgtac acttctattt gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa
     4861 atcagctgaa tcaaaaggct tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt
     4921 ggcttcagta attttttcat ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat
     4981 aactttactt taccattatc aagaatagag aacaaaaaat aagttttaaa ctgttttatt
     5041 cttatatgac ttattctgct tcctcagcaa attggctgtt atacagtttt tcataaaatc
     5101 caccttgtgc caataaactc tcatgcgttc ctttttcaat gatctgacct tgatccatca
     5161 ctagaatcag atctgcatca cggattgtcg ataaacggtg agcgatgacg aaacttgtcc
     5221 gtccctccat cacgcgatcc attgcttttt gaatcaacgc ttctaaacgg gtgtctactg
     5281 aacttgttgc ttcatctaaa atcaagattt taggatcgga gaccactgct cgggcaatcg
     5341 tcagcaactg tttttgtcct aaagacacat tgtctccctc agaattgatg gccatatcat
     5401 atccatctgg catcgttcgg ataaaatgat ctacgtttgc tgttttcgca gcatcaacta
     5461 cttcataatc tgtcgcatca agctttccga agcggatatt atctgcaatc gttccttgat
     5521 aaagccatgc atcttgcaag accataccaa atagcgaacg cacatcactg cgactcattg
     5581 atttggtgtc gattccatca atccttattg ctcctttgtc tacgtcataa aaacgcatca
     5641 atagattgat caatgtcgtc ttgccggcac cggtcggacc gaccacagct acggtttgac
     5701 cagctttcac ttcaaaattc aaatcttgga ttaatggttt cttcggatca tatgcaaaat
     5761 cgacatgatc gaatgtcaca tctcctaaaa tcgtttctgg cagtgaagca tctactttgt
     5821 tttctttttc ttctggctca tctaaaatct cgaatacacg tttcgttgcc gctgatgcac
     5881 tttgcaaaac tgaagacaat tgtgtcacgt tccccattgg ctgactgatt tgccaaatat
     5941 attggatgaa tgcttgtagt tgaccgacaa cgatcgcccc agtaataaca taaaagcttc
     6001 ctagaacagc cataccaata taagtagcat aggcagttaa ttgaactaga ggcatcatca
     6061 agccagaaat gaatgctgca cgaaacccaa agtatttcaa gctgtgatta acttttttga
     6121 atccttccaa tgtttctttt tcgcgtccat atactttcaa tacactaaaa cctgtcatat
     6181 tttcttggac gtagccgttt agctcaccaa gagaattctg catgctttga aaatatttct
     6241 gcgaagcatt gatcactcgt tttgagatcc agatcgatgt tggaatcatg atcatagaga
     6301 tcagcgccat catcggctgg atataaaaca tcattgctgc agccaacgta attcccaata
     6361 tcgcatttac gataccaata aaagcttgtt gtaaagcacc gctgactgca tctacatcat
     6421 tcgtcacacg agacaaaata tttccttgct ggtttttatc gaaatatgaa accggaagac
     6481 gattgatttt ttcttctatg tctcttctca aatcatgcat ggcactttga acgacatttg
     6541 tcatcaaaac accagaaagt aattgcgaaa tacaataccc tattccaaca actgtgatcc
     6601 aaatgagaca tttgaatata taagaaaaat tgattgattc gccattggca atattacggc
     6661 tgatttctgt agtaggaagt cctgtaacat atggcaaagc cacgttgaaa gccactgtca
     6721 gaactgtaaa taggatcact aaaagaaaag tctcacgata cggtctgata tatcgagcta
     6781 aacgttttaa ggaagaaaag gtttttttca tgctaattcc tcctttgata attgagaagc
     6841 ggcaatatca taataaatcg ggcatttttc caacaactcc cgatgcgtcc ccataccgac
     6901 gacttctcct tcatttaata cgatgatctt atctgcatgc atgatcgttc ccacacgttg
     6961 tgctacgatc aatacagtag attctgtcgt ttcttttttc agacgagcac gtaacttcgc
     7021 atccgtttga taatctaatg cagaaaagct gtcatcaaaa atatagattt ccggacggcg
     7081 tatgatggct ctggctattg ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc
     7141 ttctgataaa tgttcattaa atccatctgg ttttcgagaa atgaattctg aagcttgagc
     7201 aatctcagct gcacgttcca tttcttcttg cgtagcatct tctttcccgt atcgcaaatt
     7261 atctgcaatc gttccagtaa atagcagcgc tttttgcgga ataaacccaa tcttatctcg
     7321 caacttgctt agccgataat ctctcacgtc tactccatcg atcaagatct ttccttcact
     7381 cacatcataa aagcgaggga tcaattggat caacgtagat tttccgctac cggtacttcc
     7441 aataaaagca acggtctctc ctggagaggc tgtaaaactg acatttcgta tcactggctc
     7501 ttgcgaatgg cctggataag caaatgttac attttgaaat tcgatatacc ctttagtttt
     7561 tgtatcagat ataccatttg gatcttctac aatcgaaggt ttggcatcta gggcttcttg
     7621 gatacgtcca gctgaaacag ctgctcttgg atacatcatg aacacactag caaataacat
     7681 gaatgagaac aaggcatgga atatatactc gataaacgca atcaaatctc ctacacgaag
     7741 ctcgcctgca tcgatttgca ctgccccgct ccagatgatc aataccatta cgatattaaa
     7801 taagaaataa aaacccggtt gtgcaacagc catcaaacgg aataagcttt ttgaactaga
     7861 agcataattc tcattgacta cagcaaaacg tttttcttca aacttttcat tgacgaatgc
     7921 acgaatcaca cgcaatcctg aaagattttc ccgcaagata ctgttgattt tatctaagtt
     7981 tttttgttgc tttttagata acggttcaga caccttagca atcgctacga ctgccaataa
     8041 tagtaaaggt aatgcaccta ctacatacaa actgagtgtc acacttgtac gcatgaccat
     8101 gtacaaactg acaatgaaca tcattggtgc cataaaaccg atacgtaaaa tattttgcag
     8161 gaacaacatg atctgataag catcgtttgt tgtacgagtg atcaatgaag atacgcctag
     8221 attttcgtat tcctgatgcg aataactttg gatcttttcg aacaaatcat ctcgaatatc
     8281 tgctacgata ttagtcgtaa tcctcgaacc aaaataacct aacaagatat tcaaaataat
     8341 accgattaca gtaataacga tcataaatat cccttgttgc ttcacataat caaaatcatc
     8401 attgcggatt cctacatcga tcatacgtgc taaaatcgta ggaagtccta attcaatcaa
     8461 aataaaacca aatacgcata tgaaattgaa aaacaagaat ttcttataac gcatggtata
     8521 atgccacatt agtttcactt tctcatctcc ttttaatctt ttgttacatt ttcattacta
     8581 aaaatgatag cacaacgagc ctattaatag aacacttctc tcataaaaaa gtcattatga
     8641 ttatagcggg atgataaaca ttgatttttt ctttcaatct agttgatttc tatacttatt
     8701 tgctgtttta tgctagaata acaattaatt gttgtatatt ttttatacag aaggtacggt
     8761 tgaaaaaata tccgaacaga taagcaaaaa cgaggcttgt ctctttgact cgaatccagt
     8821 attctagtca aagcatatga aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa
     8881 aaaatcaact agctaaatca ctgctttacg cagataaagg agacaaaaca atgatctata
     8941 aagtttatta tcaagaaaca aaagtacgca accctaaacg ggaagaaaca aaatctcttt
     9001 acattgaggc aaaaagcgat gtagacgctc gccaacgtgt ggaagaaaat accccataca
     9061 atattgaatt cgttcaactt ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg
     9121 atttctcatt agtggagttt taatatgaaa ctttccatca aaaataacga gacaggcgtc
     9181 tttgctattg gtggattagg tgaaatcggg aaaaatacgt atggggtaca attccaagat
     9241 gaaatcattt tgattgatgc cgggatcaaa ttcccggaag acgatttact tggaattgac
     9301 tatgtcattc ccgactacag ctacatcata caaaacttac acaaagtaaa agccttggtg
     9361 attactcacg ggcacgaaga ccacatcggc ggaataccct acttgctgaa acaagcaaat
     9421 attcctatct atgctggtcc tctcgcatta gcgttgatca caaacaaatt agatgagcat
     9481 ggcttgcttc gagatgcaga gctccatgag atcaatgaag atacgatcat ccgttttaga
     9541 aagacttctg tcagcttttt cagaacgacc catagtatcc cagatgcatt agggatcgtg
     9601 gtgaaaacac cttctggaaa tatcgtagcg actggagact tcaaattcga tttcactcct
     9661 gttggcgaac cagcaaactt gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc
     9721 cttttatctg atagtacaaa tgctgagatt ccaacattta ctaaatcaga aaaaacgatt
     9781 gggcattcta ttttgaagat cttttcaaaa atcgaaggac gtatcatttt tgccagcttt
     9841 gcttctaaca tcttccgttt gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa
     9901 atcgctgttt tcggacgaag catggaaaat gccatcgtca acggtcaacg tttaggatat
     9961 atcaaagttc cagacggcac atttgtagat gcacacgaaa tcaatcgctt gcctgccaat
    10021 gaaacgatga tcctatgtac aggttctcaa ggagaagcga tggccgcgtt gagccgcatc
    10081 gcgaatggta ctcaccgtca aatccaaatc cagccagacg atacagtgat cttttcaagt
    10141 tcacctattc ctggtaatac aacaagtgtc aatcatttga tcaatttgct gtctgaagct
    10201 ggtgcagaag tgattcacgg aaaaatcaat aacatccata cttccggtca cggtggacaa
    10261 gaagaacaaa aattaatgct tcgtttgatg aagccaaaat attttattcc tgtccacgga
    10321 gaattccgca tgttgaaagt acatgcttct ctagcacaag atgtaggtgt tcctgcagaa
    10381 aactgcttca tcatggaaaa tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct
    10441 gctgggcatt ttgtggctgg agatgtgtat atcgatggta gcgggatcgg tgatattggt
    10501 aatgtcgttt tacgtgaccg tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg
    10561 acagtggata tcaagaaaaa agaaatcttg gctggtccag atattctctc tcgtggattt
    10621 gtctacatgc gagaatcagg agaaatgatc aatgaagctc agcgaatctt attccacgct
    10681 ttaagagaat gtttgaatgg acctgactgt tcagaatata aattgaaaga agcgatgact
    10741 ggcgcgcttc agcctttctt attcgaacgt acagaaagac atccaatgat tttgccaatg
    10801 gtaatgacac cagatgaaga ataaataaga ggttatgaaa tcagcacctt gtcctgaata
    10861 taagagaaaa caaatgaaaa acatgagaag ccatttttgt ttgttcttat tccttgatac
    10921 aaggtggctc tttcatagcc tcttaaattt atttactttt tcatacaaaa aaatacctgc
    10981 cacaagagaa attgtctctt gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa
    11041 aattgatgtc taccatcaat ttgatttaca gataaaatta agcttttaca acgtttgcag
    11101 cttgtgggcc gcggtctgat tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg
    11161 ttttgaatcc gtcaccttgg atagctgaga agtgaacgaa tacgtcgctt ccatcttcac
    11221 gtgagataaa tccaaatcct ttttcagcgt tgaaccattt tactgttccg ttttccataa
    11281 taatatcctc ctcgtgccaa aagcactaat ttaaattgta acattgctcg tgtacgatag
    11341 aggtgcaaca gatgtttcaa cttctattcc caaacaaaat tacaatataa ttgtacactt
    11401 gcttcatttt ttttgcaaga aagcaactta cttctcttcc atcattcctt cttctctcaa
    11461 actaaaatat cgtttgcgtc cgatgatcaa gtggtccagt acagtgatcc ccatcatttc
    11521 tccacacttt tgtatccttt tggtaaaatt catatcctgc tgcgatggaa taacattacc
    11581 agaaggataa tataggttag tacacgtata aaaatatctt ttatattgac acaatttatt
    11641 taacaagtat aataataatt gaaagtgaat ttaaacaaat atacaccata aacacaacgt
    11701 acctgcgaat acgttgtgtt tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca
    11761 gtaagcgcca ttgtcggctc taatgtttat ctagccaatg
//
LOCUS       DACEMO010000079        11797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEMO010000079 DACEMO010000000
VERSION     DACEMO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.081"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..586)
                     /locus_tag="IW728_001585"
     CDS             complement(5..586)
                     /locus_tag="IW728_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1024948.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IW728_001586"
     CDS             complement(769..1395)
                     /locus_tag="IW728_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1024949.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IW728_001587"
     CDS             complement(1417..2748)
                     /locus_tag="IW728_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1024950.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IW728_001588"
     CDS             complement(2903..3304)
                     /locus_tag="IW728_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1024951.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IW728_001589"
     CDS             complement(3390..3596)
                     /locus_tag="IW728_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024952.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IW728_001590"
     CDS             complement(3691..5193)
                     /locus_tag="IW728_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1024953.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW728_001591"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW728_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1024954.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IW728_001592"
     CDS             complement(5764..6381)
                     /locus_tag="IW728_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024955.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IW728_001593"
     CDS             6602..7975
                     /locus_tag="IW728_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1024956.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IW728_001594"
     CDS             8050..9162
                     /locus_tag="IW728_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1024957.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IW728_001595"
     CDS             complement(9234..10871)
                     /locus_tag="IW728_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1024958.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IW728_001596"
     CDS             complement(11039..11647)
                     /locus_tag="IW728_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1024959.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3116 a         2477 c         2130 g         4074 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttga
//
LOCUS       DACEMO010000080        11689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEMO010000080 DACEMO010000000
VERSION     DACEMO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.082"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            23..592
                     /locus_tag="IW728_001597"
     CDS             23..592
                     /locus_tag="IW728_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1024960.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            702..2456
                     /locus_tag="IW728_001598"
     CDS             702..2456
                     /locus_tag="IW728_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024961.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            2410..4182
                     /locus_tag="IW728_001599"
     CDS             2410..4182
                     /locus_tag="IW728_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024962.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            4705..4974
                     /gene="rpsN"
                     /locus_tag="IW728_001600"
     CDS             4705..4974
                     /gene="rpsN"
                     /locus_tag="IW728_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1024963.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            4989..5168
                     /gene="rpmF"
                     /locus_tag="IW728_001601"
     CDS             4989..5168
                     /gene="rpmF"
                     /locus_tag="IW728_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1024964.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            5200..5577
                     /locus_tag="IW728_001602"
     CDS             5200..5577
                     /locus_tag="IW728_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024965.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            5598..5747
                     /gene="rpmG"
                     /locus_tag="IW728_001603"
     CDS             5598..5747
                     /gene="rpmG"
                     /locus_tag="IW728_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1024966.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            5771..6754
                     /locus_tag="IW728_001604"
     CDS             5771..6754
                     /locus_tag="IW728_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1024967.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            6784..6906
                     /locus_tag="IW728_001605"
     CDS             6784..6906
                     /locus_tag="IW728_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1024968.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            6924..8447
                     /locus_tag="IW728_001606"
     CDS             6924..8447
                     /locus_tag="IW728_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1024969.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            8471..8710
                     /locus_tag="IW728_001607"
     CDS             8471..8710
                     /locus_tag="IW728_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024970.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(8903..9364)
                     /locus_tag="IW728_001608"
     CDS             complement(8903..9364)
                     /locus_tag="IW728_001608"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1024971.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(9559..9777)
                     /locus_tag="IW728_001609"
     CDS             complement(9559..9777)
                     /locus_tag="IW728_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1024972.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            9895..11511
                     /locus_tag="IW728_001610"
     CDS             9895..11511
                     /locus_tag="IW728_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1024973.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
BASE COUNT         4044 a         1691 c         2267 g         3687 t
ORIGIN      
        1 aatagaattg aggagaatat atatgaaaac aaaagattat atttttattg gtttagctac
       61 agttatttcc ctagtaattt actttttttc aataatggtt agtagtattg gtggggcgtt
      121 tggtcacagc atatccccag ggatttttgg attattatct ggaatcatat ttgtttatat
      181 tagctataac taccctagaa aaggaatttt tactatttac acaattgttt tattgttatt
      241 ttttacgtta atgggcgggg cgtacttacc ttggtttatc agttcgatta gtatggcgat
      301 cttagctgat attattctat ctgtatttgg atatgatcga gcaattcctc aagttgtgtc
      361 ttgggttcta atgcaactag gcagtgctgc cggacagtgg attcccatat ggttctttac
      421 agatcgtttt cgtcaagact ggatagatag aggacaatct gctgcaacaa tggatgcaat
      481 gatccactac gcggtgggta tatggggaat tatttcagtg ttagttgtag caagtttatc
      541 tatgattggt gtattgattg gaagaaaagt cttaaaaaaa tacaaaaaat agaacgtagt
      601 ttaccttcta tctgtttgta catctctaat ttacgctatt acggaaagca ttaacagttg
      661 taaacgaata agagaagaaa ttgaatgcgg gggaggaaaa aatgaaagta tacaaaaaat
      721 tatttagtta tgtccctaaa atgaaatact acggatggtt agccacttta ttttctgatt
      781 tttctgtcgt attaacagtc tatggatatt acagtattta caaatttctt cagtctctaa
      841 ttattgtagg agatgaatat ttagctaaga gttatgcggt tcaaactgtt gcttggttaa
      901 ctttaggagc tttattttat attttttctg gcttattttc tcatatcctt gggtttcgct
      961 tagagactaa tttgagaaaa aagggaatta gcggtttaac agaagcaagt tttcgttttt
     1021 ttgatttaaa ttcttcggga tatattcgaa agacaattga tgacaacgcg gctaaaacac
     1081 atatggcaat tgctcacttg attccagata gtgctcaagc catagtaaca cctgttttat
     1141 cagttgcctt aggctttttc attaacttta aaattggatt ggttctaatc gccatgttag
     1201 taattggtgt attcttgtta aagaaaatga cagagaatac aaattttatt caaaaatatc
     1261 aagaatcgtt agatgtgtta agctctgaaa cggtagaata tatccgcgga attcaagtta
     1321 taaagatatt tggtgctaaa gtaacatcat ttaaagcctt gaataaagca attaacgatt
     1381 atgctaagtt tgcatataag tattctttga gtgggaaaac accttttgtt ctttttcaat
     1441 ggctattttt tggcatagtt tgcattttga tagttcctat aacctttttt ataacttctc
     1501 ttaccactcc caagatctta gctgttgact tgttgatgtt gtttttttta agtggaatta
     1561 tttttgtttc tttcatgaga ataatgtatg taagtatgca cttgtttgaa gcaagatatg
     1621 cagtcgaaac actagactcc ctttatttag aaatgaagca agaccaacta aaatatggga
     1681 acgagacaca gattaaaaat tgtgacattg catttgaaaa tgtcagcttt tcatacaaag
     1741 aagagtgtgt tttagaaaat ttcaacttag ttttagaaca aaaaagaatt tatgcattag
     1801 taggaaaatc aggaagcgga aagagtacga tagctaaact aatttcaggt ttctacaatg
     1861 tggataaggg acagattaaa attggtggta agcctttaaa tcactattca aaagacacaa
     1921 ttattaacac aattgctttt gtctttcaag atacgaaact attcaatcag agtatttacg
     1981 ataacgtttc tatagcaaat cctcaagcgc cagcaagtaa agtatttgac gcgctaagat
     2041 tagccggttg cgaatcgatt ttagaaaaat ttcccgaaag agagcatacc ttaattggta
     2101 gtaaaggagt ttatctgtca ggaggagaaa aacaacgtat agctattgcc agagcaattt
     2161 taaaagatgc aaagattgtt attatggatg aagccagtgc atctattgat ccagataatg
     2221 agtatgaact acaaaaggct tttcaatatt tgatgaaaga aaaaacagtt attatgatag
     2281 ctcatcgatt atccagcatt aaatctgtag atgaaattct tgtcttgtca gaaggaaaaa
     2341 tagtagaacg tggaaatcat aatgaattaa tggctaaaca tcgagtgtat aaacaattgg
     2401 tcactaatta tgagaatgca aatgattgga ggcttgacaa tgaaaaattt ttataaaaaa
     2461 aaatttgctt taactgatca gggcgcagaa gctttaacta aagcttctat ttctagtttt
     2521 tttgtatact gcattaatat ggtaccagca ttcattataa tgatgctgat agacgaatta
     2581 gttttagaaa atgcaaagcc ccgttggctt tattttgcag tatcgtttgt tactttgctt
     2641 ttcatgtatt ggttgttaga tagggagtat gaaaacttat ataatagtac ttataaagaa
     2701 agtgcgcatt taagagtgca aattgcagac gatttgtcaa atttaccatt atcctatttt
     2761 tcaaaacata atttatcaga tttatctcaa actatcatgt ctgacgttga aggtattgag
     2821 catgcgatga gtcatgcaat acctaaagcc ggcggtatgg ctctgttttt cccttttatt
     2881 tcagtgatgc ttttggttgg taatgtcaaa atgggattag ctgttatttt gccaacgtta
     2941 tttagttttg tcttaatctt gttatcaaag aaatcccaaa cgaaagccaa tactaaatat
     3001 tacgatactt tgagagaaaa ctcggaagaa tttcaagaaa ctattgaatt gcagcaagag
     3061 attaatagct ttaatctatc taaaaaagtt caagacagac ttttcaaaaa aatggaagag
     3121 agtgaaagga ttcatttaaa ggtagaatta agtacttttt cagtcatggc cttatcctct
     3181 attttctcat atgttagttt agcagtagtc attctagtag gtgttcactt actgttaacg
     3241 ggtgaagtga ctatactcta cgtcgttggt tacttactag ccgcaataaa aataaaggat
     3301 tccttcgatt ctatgaaaga agcagtgctt gaaatatttt atttagctcc taaaatacag
     3361 cgaattagag ctatgaagga aaccagtatc caagagggat cagatagtcc tctgaaatct
     3421 tttgacattg agttgcgaga tgtctctttc tcatatgata acaatacccc aattttagat
     3481 catatctctt ttacggctaa acaaggagaa gtgactgcgt tagtaggtgc tagtggttcc
     3541 gggaaaacta gtatcttgaa attagtgtct agactgtatg attatgatga aggctgtatt
     3601 ttaattgatg ggtatgatat taaacgtgta tctccagctt ctttgtttag caaaatcgct
     3661 attgtttttc aagaggtaac tttgtttaat acaagtattt tggaaaatat acgtattgga
     3721 aatagtcaag cgtcagatga ggaagtgaaa aaagcagctc gtttagctaa ctgcgaagac
     3781 tttattgaaa aactacctga cggttatcat acattggtcg gagaaaacgg aagttcactt
     3841 tctggaggag agcgacaacg cctatccata gcacgggctt ttttgaaaaa tgcgccaatt
     3901 ttaattttag atgaaattac tgccagttta gatgcggaaa atgaaaaacg tattcaggaa
     3961 agtttaaatc gtttaataca agataagaca gtactaatta tatcgcatcg cttgaagtct
     4021 attgaaaagg taaataaaat tgtagtgatg gatcaaggaa aactagttga tcaaggtact
     4081 cataatgagt tgtatcgaag gtcagaaata tacaaaaatc tcataaagaa aactaaacta
     4141 tctgagaaat tcgtctatga aaaagaagct caatcacgat gatgaaatag gctttaaatt
     4201 aacctacaat ttttgggata tagttataat tctttttcaa aaagaataat tttattaaac
     4261 agcatctaat tttgaataca cgaaagaatc agtatgattg aattagttca cttaatgtct
     4321 attcttataa tataaggaat aatcaaagtt ttaaaaaatt ggcgataaac cagttaaaca
     4381 taaaagattg aataaaaagt tatagtttct ttcatattgc cgttttaagt agaggaaaag
     4441 ctacttaaaa cggtatttgt gtttgattgg actaataaaa gtgaattctt atttttcaga
     4501 gcctaaactg catattcttt taaatgattt gattatctct atgtaccgat aaattcgttt
     4561 tcaaatagtt atctattcat atgagtttta tttcatgtta tttattgttg acaagagcaa
     4621 ctcttgttgg taaagtatca gtgtcaaaac gtaacgatta cgatttgagg tgtctaaatc
     4681 aaaataaaag gagtataaag gtatatggca aaaaagtcga aaattgctaa agcaaagaaa
     4741 caaatggcga tgattgaaaa gtatgcagat aaacgtcagg aattaaaagc cgcaggagat
     4801 cgcaccgctc tcgctaaatt gccaagagat tcaaatccga atcgtctgag gcttcgtgat
     4861 cagacggatg gtcgtccaag agggtatatg cgaaagttcg gtatgtcccg tattaagttt
     4921 agagaacttg cccatcaagg gttgattcct ggagtaaaaa aagcaagttg gtaaaaggag
     4981 gaaatatcat ggcagtacca gcaagaaaaa catctaaggc gaagaaacgg ttaagaagaa
     5041 ctcatcaaac tgtagtaaaa ccagaaattt catttgacga agcgaatggt gattatagac
     5101 gtagccatca tgtatcttta aaagggtact ataatggaaa gaaagtaatt aaaggagctt
     5161 caaaatagtt taatatataa cttttaggag ggaatcaaaa tggaaattat ttacccacct
     5221 ttagttgaag aaggattaaa gtattatctt gaaacgaccc agcaatcgct agataaatgc
     5281 actttttatc gttcgatggt agagagagga attattactg aaacgggttt accgacccaa
     5341 caggcaattg aaaatggatt agtcaaagat tactatgagg accaaggctt atcatttgat
     5401 gattttttaa gaatctatcc aatttttgaa gagtatgacg aggagttatt tcaatgtatc
     5461 gatggttatt gggagatacc tgttgatatg aaagaaaatt tagtttcaca attggaatct
     5521 ggcgagttaa cttttgaaga tgcacaacaa attcaagcat atcttgaaga tcgataagta
     5581 ttgaaggagg aagatttatg cgtcaaaata ttattttgga atgtgtcgaa actggagaac
     5641 gtctgtatct caccagcaaa aataaacgga ataaccctga acggttagaa ttaaaaaagt
     5701 attcgccaaa attgcgcaga cgggcaattt ttaaagaagt gaaatagcta agtggtaaaa
     5761 aggtgacaaa atgggcatac cagttacaat cgtttctggg tttttagggg ctggaaaaac
     5821 aacgctaatc aatcaagcct tgcaagtctc accctacccc agggaagaaa ttattataat
     5881 tgaaaatgag tttggtgaag ttgggattga tcacaaatta ttagttcata gcgaggaacg
     5941 ggtgttacaa ctaaataatg gatgtatgtg ttgcagtctt cgcagtgatt tattagcaat
     6001 attgatttct ttactggaaa caatagaaga acatcaacaa ccaatttcac aaatcatcat
     6061 tgaaactaca ggtatagcag atccccaacc aatcattcaa acacttttaa cagcgcctca
     6121 actaagagga agagtatata tcgatagttt attgactgtt attgatagtg acagttttga
     6181 acttattgaa agagaaccac aagcccttaa gcaacttgtc atggcagatc gcatttttgt
     6241 gtctagaaaa caaaacttga atttatctgc actaaaaaaa ttacagagag aaatcaaaag
     6301 tattaaccct ttatccgata ttcgaatgtt cacacatagt gaaacgattc aaaaaaagga
     6361 tttttataat ttgaaaaaat ttaatcatcc cttagttttt gaaagtgatt catcagaaaa
     6421 tcactataat ggcaaatact atcatcaaca ccatcaagta catgagttta aatcaattct
     6481 cttaacttca gatcaagaca taaaaaaaga ttttttactc caatggattg actggctgat
     6541 tttaaccaac caagggagcc tgtatcgttt taaaggattg attgctttac aagagcagga
     6601 ttttatagtt gccatgcaag aggtaaacga acaggttaat tttcaataca ccactcgaca
     6661 aaaagaccaa accactatta ttctaatcgg aaaaatgttg gatgaagaaa ggatcaaaca
     6721 gagttttgat aaattactgg cggaaagcaa ataatgataa gtagtgattg ggggtaattt
     6781 ctaatggaga aaacggatat atctagtgca tatcgacgac ttaaaagccc gaatattaaa
     6841 actagaaaaa gggcattaaa aattatcaaa gaacataaac gaaataaaat gaagaaatta
     6901 gcataattaa ggaaggaaaa agaatgaaga aaatcagtgt aggacttatt gggatagcag
     6961 ctctaggatt attgggagct tgctccagca ctaatgatgc aaaagtatcg aatgataaag
     7021 atggaaaatt agaaattgta acaacattct atccaatgta tgatttcacc aaaaacatcg
     7081 tcggcgatga agcgaacgtg gatttaatgg ttccagcggg atcagaacct catgactatg
     7141 agccctcagc taaggatatg gcaaaagctc atgatgctga tgtttttgta tatcataacg
     7201 aaaacatgga atcatgggta ccaaaagcta aagaaagttg gaagaaagct gggcctaatg
     7261 tagtagaagg aacaaaagac atgattttat tgccaggtag tgaagaagaa gatcatgatc
     7321 acggagaaga agatcaccat catgaactag atcctcatac gtgggtttca cccaaaatgg
     7381 caattaagga agtttcaaat atcaaggatc agttagttaa attgtatccg aaaaaagcaa
     7441 aagtatttga gacaaatgct gaaaagtatt tgactaaact gaaacggttg gatgctgact
     7501 ataccactag tcttaaagaa gcgaaacaaa aaagttttgt gacgcaacac gcagcctttg
     7561 gatatttagc tttggattat ggattgaaac aagttcctat tgctggacta agtcctgaag
     7621 aagagccatc ttctggtcga ttagctgaat taaaagagta tgttaaaaaa aataagatca
     7681 attatattta ctttgaaaag aatgctaatg ataaaattgc aaaaactttg gccaacgaag
     7741 ctgggataaa attggaagta ctgaatcctc ttgaaagtct tacaaaagaa caaatggata
     7801 atggtgaaga ttatgtttca gtaatggaag acaatctaaa agcgttagaa aaaacaacta
     7861 tggttgctgg tgaagaggta gttccagaaa aagaagcgaa ggatgaaaag acagttgcta
     7921 atgggtactt taaagatgcc gatgtgaagg atcgcgaatt gagtgattat acaggcgaat
     7981 ggcaatctgt ttatccgttg ttaaaagatg gaattttgga tgaagttttc gattacaaag
     8041 caaaaatcaa taaagacatg acagctgccg agtacaaaga ttattatact actggctata
     8101 agacagatat tgatactatc aacattaaag acaacaccat tgattttgta gtcaacggtg
     8161 aacatcatca atacacatac aaatatgttg gatacaaaat tttgaattat gaaaaaggaa
     8221 accgtggtgt tcgttttaat tttgaaactg atgatgcagg tgctggtcgt tttaaatata
     8281 ttcaatttag tgatcatgga attgcaccaa gtaaggcaga gcatttccat attttctttg
     8341 gtggagaaag tcaagaaaaa ctctataatg aattgcataa ttggcctact ttctatccag
     8401 cttcattgtc taaacatgaa attgctcaag aaatgatggc tcattaaaat tcaatttttg
     8461 gaggaaatac atggtaagaa aagtattatt gtctatggat tccaagaata ttgatgctta
     8521 tcggcgttat cgatttgcaa agcaaatgag attaaatggg agtacattag ctgatattgc
     8581 tgcaacttta catgttagta ttgacacagt tcatagagta ttaaaacggg atcctgattg
     8641 ttgttttatc aaggtcaata ctggaagaag tagacgttta gcaaaacggc ataatagctt
     8701 ggccaattaa ttgataatac cagagtagat catttttccc ttatttggat ttgatctgct
     8761 ttttattatg tcttgaattg tcaaatcaag cgcaacactt tatcaaagaa gacttcataa
     8821 ggtgttctcc aatctaaaca ctttctaggt ctgttattca gtttttcaat catctgattt
     8881 atcgttgagt tatctaccgt agctatctct ttcgattttg gcaagtattc tcgaatgagt
     8941 ctattcgtat tttaatttgt tcattgttgc caagggcatg aggatctgga aaataaaagg
     9001 gcatattatt caatgcttct gtaactgcga aatgattgga gaattctttt cctctatctg
     9061 gagtgatcgt tttgaccgcc tcatttggaa tactgcttaa aagttcaatc attttctcgc
     9121 tcacataagc ggctttcttt ctggggacct tctttcctgc taaaaggaag catgattttc
     9181 tgtctgtcaa agtcactaaa caagtaccac cagttttgcc agctacaata tcggctttcc
     9241 aatgaccaaa atcacttcta tcatctgcgg attttggacg gccatggatc gaatgagtaa
     9301 ggcaaatctt tccacgagac tcgacatgat tttttgtatg tcttgttttt cctcgatggt
     9361 gcaatgaacg gatcaggcct ttattgccgt gtgagagggg ttctgtatcg aaaagatcat
     9421 tgcaaattgc acgttagatc gtgataaaac taattgaaaa gtccatatgt tcatgcttca
     9481 aacgattcga gatttcctct ggcgaccact gaaaatcgac aaataaatga cgaacaattg
     9541 accaaactca cggatcattt agaatgtgat aacgtccgca tttttttctt cgataagcat
     9601 aatttttttc cgctttggaa ggtgaatagt ttttttcttg tttcttctca gttcatgtaa
     9661 aatagtaaac gggctatgct gaatgtcgtt gccaattata ctaatagaaa atccttcttc
     9721 gtgaagcaga aagattcttt ctcgttcgag tatagtaaga tgacgatact ggctcataat
     9781 tattttcctt ctaggagtgt tctcgacaaa cttatactag ttggatatcg attatgactc
     9841 atttttatgt gttgcactta aagtgtaaat tcaaggtata gaaagaggtt ccctatgaaa
     9901 aaaataccta ttacaatctt aactggctat cttggttcag gaaaaacgat acttctaaat
     9961 catattctgt acggtgatca cggaaaggaa attgctgtca ttgtaaatga cataggggaa
    10021 gtaaatgttg atggggtatt agtcgacaag aaaggtttta aaagaactgg agaaaaactt
    10081 gtttcgatgc cgaatggatg catttgttgt acattacgcg aagatttgat tgttgaacta
    10141 gatcaattag tgcaattttc tgacttgaat caaattgtta ttgaagcttc aggaatcagc
    10201 aaaccaattc caattattca agcaattata ttagctaaaa accctttaga cattgatttg
    10261 acccaacgat tagagattgg tgccattatt atagtagtgg atgtaaaacg tttttgggat
    10321 aatttcgaaa cgggtgaaaa gattcaggaa agaaaagctg aaggtaatgt agttgaagaa
    10381 gaacaggata ttcgtggctt gttaattgat caattcgagt gttgtaacat attactttta
    10441 aataatagtg acttagtgtc aaccaagacg atttacaaaa ttaacggtct aattgctaag
    10501 ttacagtcca atgccaaaat aattaggaca attaaaggtg aagtagactt atcgaaaatt
    10561 ctaaatacaa atctttttaa tatagatgag acttcctatt cagtgggctg gttcaaagag
    10621 ttagagcttg gctatcaaaa tcatattcct gaaacagaag aatatggtat ctcaagtttt
    10681 atctatcgaa atcggttact ccttgacgca caaaaatttg cccatcttac ggataaaaat
    10741 tttcctggca atattacaag agcaaaaggg tttctttggt ttgcccaaca tagtgatttt
    10801 tcgacgatct ttgaaacatc gggtagctat aaaagtattg aatcattaaa ttattgggta
    10861 gctagtttac ctaaaattga gtgtcaaatg attatacaaa aaaatcccga attgaaggaa
    10921 gcctggcatc cgttgtttgg tgaacgcatt aataaactag tttttattgg tatgaagatg
    10981 gatcaacaat gtattaagga acaacttgag ggttgtttaa ctgattctct taatgcagat
    11041 aatttccgaa aaatttcacc atttccatgg aaaaagtcta atatgtttag ccagatatcg
    11101 ttaatgaaat ttttaagaaa agctggtgtc aagataggcg atgtcataat tattggcatt
    11161 ttgattatta cctcttttat tcccttattt gtattctcta tagaaaagga gaatgaaaca
    11221 actgaagatg ctatcaaata tgccatcgtt cgaattgatg gcaaggaaat ggatcgcttc
    11281 gatttggata agatcaatca taaactcgtg aattatcatc ctgcgaaggg acaatataat
    11341 attgttgaga ttaaagatgg aagaattcga gttaaagaag ataatagtcc tgatcaaatc
    11401 gcggttagaa ctggatggat ctcaaaaccc ggtcaaacga gtatttgttt accccataag
    11461 ttagttatta gtattgagaa aaaagaaagc acagattact acatttacta atctggaaat
    11521 agatgaggag gtttttaata agaaaaagat attattatta ataatagcag gggctttatt
    11581 aagtgtgtta agcgcttgtg gacaagaaaa agaagcggtg acaacaggca caagtatctt
    11641 taacgggacc tgcaatggtt tagttaacag tcggttagat tacttattt
//
LOCUS       DACEMO010000081        11676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEMO010000081 DACEMO010000000
VERSION     DACEMO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.083"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(347..2545)
                     /locus_tag="IW728_001611"
     CDS             complement(347..2545)
                     /locus_tag="IW728_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1024974.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            2777..3769
                     /locus_tag="IW728_001612"
     CDS             2777..3769
                     /locus_tag="IW728_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1024975.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            3829..5250
                     /locus_tag="IW728_001613"
     CDS             3829..5250
                     /locus_tag="IW728_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1024976.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            5243..6742
                     /locus_tag="IW728_001614"
     CDS             5243..6742
                     /locus_tag="IW728_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1024977.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            6781..8175
                     /gene="uxaC"
                     /locus_tag="IW728_001615"
     CDS             6781..8175
                     /gene="uxaC"
                     /locus_tag="IW728_001615"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1024978.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            8334..9017
                     /locus_tag="IW728_001616"
     CDS             8334..9017
                     /locus_tag="IW728_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1024979.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            9019..9848
                     /locus_tag="IW728_001617"
                     /pseudo
     CDS             9019..9848
                     /locus_tag="IW728_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            9859..11613
                     /locus_tag="IW728_001618"
     CDS             9859..11613
                     /locus_tag="IW728_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024980.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         3691 a         2259 c         2198 g         3528 t
ORIGIN      
        1 tcgaagaatc tactcctgga acaccgtgta tcagaggttg tgaaagagac gttcagcctt
       61 gagcgatgat aaaaactctc acaaacattg cgacgagtaa cccacaggag caataagaag
      121 cgattttgaa aaatagcttc ccatatttca caaaattatg ccttattgtc gatagcctgt
      181 ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa gacttttgtc acagttcttt
      241 ttgcgtgctt agccttgaaa tttaactatc cataaataca aatactaggg ttattctaac
      301 ttcttttatt gcttgaatca gtccacaaga aaaacagcct cttgattcac aaatattttt
      361 ttcgatattc tcccggcgta atcgattcct tctttttaaa gaaacgaata aaattttgcg
      421 gatttcgata ttgcaatctt tctgcaatct cttttacgga gaggttagtc tctttcaacc
      481 atttttttgc tacctctaaa cgatgatttt gaacaaaatc accaaatttt tctcctgttt
      541 cctttttaaa tacattactt aaatagttag ggttataatg caacctatca gcgatcacat
      601 ctaaagacag atcacgatca tattctgtgt agacgatatg cataatattc tccgacaaat
      661 ttttaaattc atgatcagtg attgcttgag agcattctgt gattggcaga actaattgtt
      721 ctaaaacaag tttttctagt tctttgggat gataattgtt cagtgctttt acataaattt
      781 gtttcatgtt atcaaagttt tttgaatcta ctcccaataa ctgtcctaat tgaacgagtt
      841 cattgatcaa gcggaccgaa actacttccc gactcaaagg atttttattt tgtgcaaaca
      901 atgcatcaat caattcatgt actaaattct tcacttcctg atttccagaa cgaatggctt
      961 caaacaatcg attttgttct tctatcggat atctaataag tgttttttca tgagcagcag
     1021 gaacgatttc ctgataaaaa ataattgaac ttggaccagt atttacacga taatacaggg
     1081 cttcttttgc ccgatcaacc gattgcttgc tttcattcag cgtttcgaac cggttactga
     1141 tgcctatact gatagttaaa ttcaagtatt cttttacacg tttttgaatg agtcggcaat
     1201 actccatgac ttttttgctt gcttctgcat catctttatc cattcgataa atagtggctt
     1261 gcatttcttc attcaaaacg atcggcagca ttcgattttc ctttggcaca atttccgtga
     1321 tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc accgtgacta tcttctaaca
     1381 gatcgatttg aaccaaagct gtataatagc agtcattttg agaactataa ccaaattgct
     1441 gcaaccgctg atctagttct cgcctttcta ctcgatttct aaataaacta agtacaaata
     1501 gcgtttccag ctgtggcttt tgggtaacta agtgtgcact taacgcttct ttttctccta
     1561 tgatccgact gactgattga gcgaccaaat ctagttcttt cccactgaat ccttttgatt
     1621 taagattcag acgttcctgt atttgagaaa tcggacgggc aaatctgtcc gaaaataagt
     1681 aggaaagaag accaatgagt cctaccagtg ttaaagacac tgcaactagc ccgattctca
     1741 gattgcggat cattgcgcca attgcactag agtcaacttc taatctgtaa atccaattat
     1801 tataatctga tttcagtact atatacgttt tatctttttc tcttatcact ttcagctgat
     1861 tgtaatctag atccacgtcc gttaatacag ttggttgctc tttatttttt actgtctgag
     1921 tagtaaaaag gttgccaact tcgttttcaa tagaaagtat gtcttctcca ctttcaccaa
     1981 caacttgttc tattgtatgt ttctctatcg tagcaattcc caatgcatat ttttcttttt
     2041 caaacattgg caaagaaatc accatttcga ttcctttgtt tttcttttgc caaaataaat
     2101 tatttttgtt atttatgtac ttctgttgat acttttcaat ttcctcttcg gtcagttgac
     2161 tcaacgatcc attgattact ccccatttac cagccaaact gactaaatca taattattat
     2221 catccataac aatcgtttct ataaaattta gttctttcct gatttcagaa taagtggagt
     2281 aatctgtata tgtcagcgga tgcttgaata tgtccgagaa atttttggtc gaaccatacg
     2341 tagaaaacga atattcaatc gttctaattt tccattcaac gttagtttta acttgcctga
     2401 gcaaattctt tttgtctgtt aaataactcg attcgatcga ttcataggta ttctggtaaa
     2461 tgaataaact aaatcctatg acaagtacgg ctcctaataa aaacatcggt aaaaatattt
     2521 tataaaatac tgtacttttc ttcattttct tcctctcctc cttttaaaat aaaaggcttt
     2581 catttttctg taaaattgat actcatctaa ttaaatgagc attccccgct ttcaagaata
     2641 gcataattaa aaataaaaat ccaagtaatt tttaacgtgt gcaatctttt catatcaaag
     2701 atctattgac ttatcgtagt tatcgtaata tgatgaatgc gttaacaaaa agtgaaaaag
     2761 taaggaaaat gatggaatgg taacgatcaa agatgtcgct aaagcagctg gcgtttctca
     2821 tacgacagtt tctcgtgctt tgaataacag cagtttaata aaaaatgaaa caaaacaaaa
     2881 aatacgacaa ctagctaagg aaatgaatta ttcgccaaac tatagtgcaa aaggtttagt
     2941 gaatcaaaaa aaatatctaa tcggactatt tttctcaagt ttgcataaag gaacttcatc
     3001 aaatttccta gcagattcca ttgcaggcat ccattcagta ttagacacaa attacagttt
     3061 atctgttgaa agtatcgatg ttgttgattt aacagaaatc aacctgcaac gctacgatgg
     3121 actgatcgtt atgagtcaaa gtgatgatga tcagcctttc atcgactatc tcaaagaaaa
     3181 agaatttcct ttcgttgttg tcaatcgtca catcaatgat ccagcggtta tcaacgtcac
     3241 tgccgatgat gcggccggtg ttatgcaggc agtagattac ggtattggat gcggacatac
     3301 gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg acaaaagaac gggaaaaagg
     3361 ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat ccagcctatt ttttaacagg
     3421 agattacagc ttagaaagcg gcctccaaaa catggaatac ctattatcac tttctgacct
     3481 gcctactctg gttttttgcg gcaacgacga catagcgatt ggtgcattga gagctgtaca
     3541 tttagctggc ctccgtgttc cccagaacat ttctctgatc gggttcgatg attctccagt
     3601 cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag cctttgaaag aaatgtgtca
     3661 agaaggaaca aagcttttac tagatttgat cgaaggcagg acgatcgaac aatctaaaat
     3721 tgagctctcc accttgttga tccttcgtga gtcgatcaat tacttgtaaa ctacaagtaa
     3781 ttttatataa aaatgttaac gtgtgcataa cacagaaagg aagtttatat gaaaaaactg
     3841 acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag ttcttcagtt tggtgaaggc
     3901 aattttatgc gtggatttat tgattggcag attcagcagc tgaataacca acaattgttt
     3961 aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc ttggaaatat gatgaaccag
     4021 caagataatt tatatacggt cattttacaa ggactcagca atggcgaaat cgttgatact
     4081 tccgagatta tcaccgtagt agaacaaatg atcaatcctt atgatgaatg ggatgcttat
     4141 ctatctctag cagaaaatga cgacttggcc tatatcgtct ccaatacaac agaggcagga
     4201 atccaattcg tggagactga taatttaaca gatactcccc cacagagcct cccgggcaaa
     4261 ctgacagcct ttttataccg acgtttcaaa ttaaacaaag ctggctttac catcattcct
     4321 tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa tcattttacg ctacgcagaa
     4381 aaatgggatt tagaacctgc ttttgttgag tggttagaaa aagaaaatgt tttctgttgt
     4441 agtttagttg atcgcatcgt cccaggttat cctcgcgaag atgcaggtgt tttcaatcaa
     4501 cggcataact atgaagatca attgatggtc aaagcagaac cattcatgct ttgggtgatc
     4561 gaagggccta aagaattgga gaatcaacta ccattcaaac aggctggctt gaatgtcatc
     4621 atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc atttgcttaa tggaccgcat
     4681 acagcgatgg tccctcttgc tttgttggcg gaattggaaa ctgtagaaga agtaatgaaa
     4741 gatcctttat tctccgcata tgttgatcag ttattcaatc tagaattgat tcctatgctt
     4801 tctttaccta aagatgaatt agccatttat gctgaccaaa tcaaagaacg tttcttgaat
     4861 ccttttgccc atcacaaatt agaagcgatt tcattaaata gtgtttcaaa gttcagcacc
     4921 cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa atcaggtacc accgttaatc
     4981 actgtttcac tagctgcgct acttcttatg tatcgaggcg atcaagtaaa gccacatgat
     5041 gatgaaaaga cgataagcga atttactgat gcttggtctg ataatggaac agctattcct
     5101 cgattattgc aaaacgctgc gctttgggga gaagatttgt ctcaaatacc gaatgtgaca
     5161 gatacagttc aagaaatagc tgcaaagatc gatcaaaaag gtgtccggtc agtattacaa
     5221 gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa ccaacaatga aattaaatcg
     5281 acgagacagt gtggtcgtcg cattaacccc tattccaaaa caaacacaac tgacgattga
     5341 tcagcaaacg atcaccactc ttgaagatat cccgcaaggt cataagattg cattgggtga
     5401 tttgaaagct ggagacaacg tgatcaaata tggctatcca atcggtcatg tgacgacaga
     5461 cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa accaatcttt ctggtgaatt
     5521 ggattatcga tatgaaaaag atgtccatcc aagtcattac ctttttgaga accgaacatt
     5581 tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt aatgatttgt ttttagtacc
     5641 taccgttggg tgtgtgaatg gcattgctga actgatcgtc aaacagttca aggaaaaaca
     5701 tccagatctg ggacagtttg accatattac gattttaaag catccttatg gctgttcgca
     5761 gcttggaaaa gatcatcaaa atactcgaga aattttagcg gacgcagtaa atcatcccaa
     5821 tgccggagga gttttagtat ttggattagg ttgtgagaac aatacagttc ctgaattcaa
     5881 aaaaatttta ggtgaatatg atgaagaacg agtgaaattt ctagtggctc aagatgttta
     5941 cgatgagatc gaacaaggtg tggctctatt agaagagctt ttaacagcgg cggaaaatga
     6001 ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt ttgaaatgcg gcggttctga
     6061 tggtctatcg ggaataactg ctaatccttt acttggtgca ttttctgact acttgattgc
     6121 tcaaggaggc agcacgatct taacagaagt gccagaaatg tttggcgcag aacaagtatt
     6181 gatggcacga gcggaaaatg aagaagtttt tgactcaatc gttgacttga tcaatgactt
     6241 caaacagtat ttcttgtctt acggggaacc agtttacgaa aacccatctc ctggtaataa
     6301 agcaggtggg atcactacat tagaagataa gtctttaggc tgcacgcaaa aatcaggaag
     6361 ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc agaaaaccag ggcttagttt
     6421 attgcaagct cccgggaatg atcttgtagc tgcctctgct cttgcttcat ccgattgcca
     6481 gttggtcttg tttaccacag gaagagggac tccgtttggc tcatatgtcc caacggtcaa
     6541 ggtatctacg aatacaactc tttttgaacg caaaggtcat tggatggatt ttaatgcagg
     6601 cgtcttatta gaacaaccaa tggaagtaat cttagaatcg tttgttcaaa aaatcatcgc
     6661 tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac gttcgagaaa ttgccatttt
     6721 taaaaacgga gtaacacttt gattttcata aaacagtata aaaagaaaga aggaagattt
     6781 atgtttttat ctgatgattt tttattaaaa gacgattggg cgaaaaagct ttaccattcc
     6841 tacgcaaaaa acatgcccat tattgattat cactgtcact tagatccaaa agaaatttac
     6901 gaaaacaaca atttcagcaa tctgacagaa gcttggttag gaggcgatca ttacaaatgg
     6961 cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca caggaaatgc ttctgatttt
     7021 gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta tcggaaatcc gctttacagt
     7081 tggtcacatc ttgaattgaa gcgtttcttc ggtattgact tgttgataaa tgaagaaaat
     7141 gcagagaaaa tttggacaca agcaaatgaa attttggcag ctcctgattt tcgccgtcgg
     7201 gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa cagatgatcc cgcagacgac
     7261 ttgcactatc atcagttgct tgctcatgaa gaaccagatt tgaaggttct accttcattc
     7321 cgtccagata aagcactcaa tattgaaaaa gaaggattta atgcatgggt cacactactg
     7381 gaagaagcag ctgaccaaac gatcaccacc tatcaagaac tgatagaagt cttagggcag
     7441 cggatagaat acttccatca acatggatgt cgtgtttccg accacgcttt agacatctta
     7501 cgctataaag aagcggatga aacagtatta gaagagattt ttcaaaaagc acgagctaac
     7561 aaactattat cttcagatga aatcgatgct tatcgtacag aaacactgat acatttgacc
     7621 cacttctatc attctcataa ttggacgatg cagctgcaca tccatgctta tcgtaactgt
     7681 aatacacaaa tgttggaaaa attagggccc gataccggct atgatggaat gaatgatctt
     7741 tctttgacta ttcctctgca aaaattattg aaccgtgcag aagaaacgga tcagttgcca
     7801 aagacgatcc tttattcatt gaatccgaat gactatcctg cgattttagc tttgatgggc
     7861 tgtttccaaa aagaacagaa tggaaaactg cagttaggtt ctggatggtg gtacaacgat
     7921 actcgagctg gtatgcgcga tcaattgaca caatttgctg atggtagtgc tttggggaat
     7981 tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct acactagaca tgaatatttc
     8041 cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc gaggagaagc acctgaagat
     8101 gaacaacttt taggaagtct agttcaagcg attagttata caaatgctaa aaattacttt
     8161 ggcttttttg cctaattgat taatagtaac agtaacgaat caaaaacagg atagtgaaat
     8221 aacggaatcc gtttatccac gttttagaga agaagacgga ccaaatttgc tccgtcttct
     8281 tctcttgctg ttctgcttca ttgcctataa acctttatga aagaagagat aacatgccta
     8341 ctatccatat tgccggtgat tctactgcgg cagaaaaaga tgaaaacaag cgtcccgaga
     8401 caggttgggg agaaaaaatc tctgcttatt tttcacccga gatccagatc aataaccaag
     8461 caaagaatgg acgcagcacc aaatcattta tccaggaagg aaggttagcc cgtctagctt
     8521 cacagtttca gccgaatgat ttcttatttg tacaatttgg acataacgac cagaaaatcc
     8581 aagaagaaag aggtacgctt cccttcggta gctatttaaa aaatctgcag atattttttg
     8641 aatgcgcaaa aaaagcgaac gtccaaatgg tcttactgac atctgtgacg cgacgagatt
     8701 acttagaaaa cggaacattg aatccagata tacttggaga ctatccaaaa gcgatgcgtg
     8761 catttgctga gaaacaccat attcctcttt tagatgtgtt ctcaagatca caagaattgt
     8821 tccagacctt ctcaaaagaa gagacaaaaa aattctacct gcatcttata cctgatactt
     8881 gtaaaaatta cccagaagga ctaaaagaca atacccactt tagtccagaa ggcgctgata
     8941 aagttgcccg aataattgtt gaattgataa aagaatcccg tctgcctctt gctaattatt
     9001 tacaaaaagg agtttgagat gtttccatta aatttaggca tccgtgccca cgatttagat
     9061 gttcgaaatc gtgaagaaat cgttcaaaaa gtatcacagc ttcacttgtc ccatatccaa
     9121 tttgcaccac aaaaagcatt tcctgaacaa cttaaattaa ctgacatgac acttgggagt
     9181 gctgcctatt ttggtgaata cttccaaaaa aacaatatcg aactttccat tttaggatgc
     9241 tatatcaacc tatccagcaa aaatcaagaa gttcgtgcac aagcattggc aactttcaaa
     9301 cagcaaattg ctctgcctat caagccaaga tgattgctac ggaaaccggt agtgtctctg
     9361 tcggttacac aaaagaaaat tttactgaag aagcttatca gatcgccaga aattctatca
     9421 ttgaattagt cgctcatgca gaaaattttg gcgtaactgt tgctgttgaa gcagggatca
     9481 atcatccaat ctataactat cagttagcta aacgtttaat cgatgaagta gcttcaccaa
     9541 atttaaaaat cattttagat tgtgcgaatt tgatgcataa agaaaatcat gctgatcagg
     9601 aaaaagtgat ccgaggtgcc ttggacaatt tacatggtta tattacagcg cttcatctca
     9661 aagactatat catggaaaat ggaaagatcc gcatggtacc tgttggcaag ggctgtttag
     9721 atttccgccc ggtactgcat tatatcaaat atgagcgtcc tctaatgtac gccactcttg
     9781 aagctactcc tgagatttat gttcctgaag caatagaaca cttgcagttt atttaccaag
     9841 attgttaagg agaagattat ggaggaacga atacagaaaa gttatcaaaa atatcatgca
     9901 aatgcttttc agacattgct gcatctttat cagggaaatt attggcgttt tgccatgtca
     9961 ggactctttt ttattatcaa acatcttcca tcttggttaa tgccgatcgc tattgccaat
    10021 gtaatcaatg cagcttcatc gaaagattct tctcagttgc aggccattta tttcaacgct
    10081 tttttgatgg cttgtctgat tggtcaaaat atattgacga attattttca tgtaaaatac
    10141 catagcactt cgatccgtca agtagaagct ggactaagag cgacccttat caagaaaatc
    10201 caagaactat ctattcctta tcaaaaagaa ttgcaatctg gacgaatcca gtcaaagatc
    10261 atccgtgatg tagaagctat ccaaacgttg tcctctcaag tatttgtcag cggattaaat
    10321 atcgctgtca atttattcgt agctttaggt atcacgcttt ataaaagtcc tgttgttttc
    10381 atgtttttct tattgactgt tcctctcgcc gcattcgtta ctctcttttt ccgtaaaagg
    10441 atcagacagt ccaactcaga cttcaggcaa gaaatggaag agacttctgc aaaagtgatc
    10501 gaagtgatcg aattagcacc gattgctcga gctcatgggt tagaaaaaca cgaaacaact
    10561 cgcttaaata agcattttac tcaaatctat gaaaaaggat tccacttaga tatcctgcaa
    10621 tctgttttcg gatcagtcag ctgggctagt tttcagatat ttcagctgat ttgtttgatt
    10681 ttcactggga tattagcttt acaagggcgc atccaacccg gcgatgtcgt gatgtatcag
    10741 acgtatttca cgacgatcgt caattccgtt tctggaatga tcacgctgat tcctacgatt
    10801 tcaaaaggca tggaatctat cacttctatt ggcgaactgt tgattgctgg cgatgtagaa
    10861 acttatcacg gtaagaaaaa aatccaacat ttggatggaa attttcgttt tgaggatgtt
    10921 tcttttcttt atccggactc actcaaaccg attatcgatc atttttctct agaggtgaaa
    10981 gctggagaaa caattgcttt tgttgggcca tctggctctg gaaaatctac actccttaac
    11041 ttattgattg gatttatcca gccgacttca ggaaaaattt ttctagatga tcagcctttg
    11101 gatgaactgg atatgcgaag tgtgcgaaat tatttagccg ttgttcctca aaccactttg
    11161 ttgttctccg cttcgatcaa agagaacatc acttatggct tgaaaaacgt atcaaaagaa
    11221 cgtttagatg aagtcatcga agctgcgcaa ctttcttcac tgattgacca gcttcctgat
    11281 ggtctggata ccttagtagg cgaacatggc aacaagctgt ctggtggcca aaagcaacgt
    11341 atctccattg cacgagcgtt gatacgtcag ccaaaaatca ttttgctaga tgaagcaacc
    11401 tcagctttag ataaccagtc agagaaaaaa atccagcaag ccctgaatta cctgacagaa
    11461 acacccacta cttttattgt ggctcatcgg ctttcaacga tcaaagaggc agataaaatc
    11521 gttgtgatcg atgaaggaaa aatcgttgaa ataggtactt acgaaaaact gttagctcaa
    11581 aaaggctatt tctaccgctt gaaaaatgaa tgacaaaaaa taggagctat gacaagactt
    11641 ttgtcgtagc tcctatttct gaataaacgg tgttct
//
LOCUS       DACEMO010000082        11553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEMO010000082 DACEMO010000000
VERSION     DACEMO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.084"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(133..2031)
                     /locus_tag="IW728_001619"
     CDS             complement(133..2031)
                     /locus_tag="IW728_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1024981.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(2192..3454)
                     /locus_tag="IW728_001620"
     CDS             complement(2192..3454)
                     /locus_tag="IW728_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1024982.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(3447..4346)
                     /locus_tag="IW728_001621"
     CDS             complement(3447..4346)
                     /locus_tag="IW728_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1024983.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(4491..6269)
                     /gene="uvrC"
                     /locus_tag="IW728_001622"
     CDS             complement(4491..6269)
                     /gene="uvrC"
                     /locus_tag="IW728_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1024984.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(6451..6765)
                     /gene="trxA"
                     /locus_tag="IW728_001623"
     CDS             complement(6451..6765)
                     /gene="trxA"
                     /locus_tag="IW728_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1024985.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(6852..9212)
                     /locus_tag="IW728_001624"
     CDS             complement(6852..9212)
                     /locus_tag="IW728_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1024986.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(9327..9875)
                     /locus_tag="IW728_001625"
     CDS             complement(9327..9875)
                     /locus_tag="IW728_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1024987.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(9933..10370)
                     /gene="zapA"
                     /locus_tag="IW728_001626"
     CDS             complement(9933..10370)
                     /gene="zapA"
                     /locus_tag="IW728_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1024988.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            10572..11489
                     /locus_tag="IW728_001627"
     CDS             10572..11489
                     /locus_tag="IW728_001627"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1024989.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
BASE COUNT         3276 a         2451 c         1955 g         3871 t
ORIGIN      
        1 tcatgtttgt tgaatagtgt gacttgtgac cgaggagttg gcagaagaac accgtttatc
       61 cggaaaaaga gactgcgaca cgacttttgt ctcagtctcc agtctatgga acaatgttca
      121 ctggaattct tctcaatcgt cctttcgata gtccaacagc cttttgagct gggtaatctg
      181 ctgctgcagt tccatacctt ttgttgtatc acgattgagt ttgcgtggta gttgctgttc
      241 gattactttt ttcacatttg tttggtcgga tttttttgca aattgttgtt caataggcaa
      301 aaacggataa tgtgtcacga tttggaaacc ataagaattg cataatagtg aataaccggc
      361 aattcctgtc gttttttgat aagctttgga taaacctccg tcaatgacaa atacttttcc
      421 atttgctttg atcggtgact cccctttttt tacttttact ggcgtgtgcc cattaatgat
      481 tgctgaccct tcttcttttg tgtcgaattc tgctaaaatt ttttcactga accactcttc
      541 atctcgcaag tgataataag gattgctttt ctcgacgtgc gtatgaggct ctgtgagaaa
      601 ataacgttca aaagtcgcca ttttactctt gccgaataaa ggcgaaaatg gcccactcca
      661 cgcataccaa atcaaatcgg ttgctaaatc ttcttttgca gtcagatctt ttgcgctttc
      721 agttatctgt ttttcaaaaa agcccagcag ttcttttccg cgataagatt ggtctaattc
      781 aaatgctaag agctgtccct tctcgtctac aggtatacag ccatgaaaca gcacatgctg
      841 attgttcgtg agatacattc cccctttttc gatcaaaaac gtcatatgtt tttgcattct
      901 caaagagtgc tggaatgaat acatcaaact atcaatcact ttttgttcct cttccaagaa
      961 tttttttgga tcatctggct ggatcgtttg aaaacaagtt cctttcaact catagagctg
     1021 accttctaaa tcaatcgttg atgcttcata gtcgattttt tccagcaatt tacgatcgtc
     1081 cattttaaat tcaggtcgac gatccaataa ctgatgttct gatttgaatt gaaggataga
     1141 aagtgcctga tggatttttt ctaattcttc ttgctctctt tcggatagat ttttactgtt
     1201 tttaggcgca aatactggat tcttctgata agtttgatga gcaaaagaaa acaatggacg
     1261 taaattcaac ccatattctt tctctaattc aaataaataa ttatagcgtg ctgcaatccg
     1321 taaaagtgtc agtaaacatg cctcagagcc gcaatacgca ccaatccaca aaatatcatg
     1381 atttccccat tggatatcta cagaagaaaa atccatgatt cgatccatca ctttggctgc
     1441 ttgagttcct cggtcaaaaa tatctccaac gatatgcaaa tgatcgatga cagatttttg
     1501 gatcgtctgt gcaagtttcg ttaaaaattt ttcttcttcc ttcatcaaaa caagttgttc
     1561 aatgattttt tttacatagt ctttttttcc attgatacgg tcatcgatat acaagagttc
     1621 ctcaatgata tagctatatt ctttcggcaa tccttttcga acttttgacc gggtatattt
     1681 gactgtcgta aaccgtaaaa gatcgatcaa atgatggatc agctgcgatt tatcttgttc
     1741 tttgtaccaa tcagtcgtta atgcatattc tggataagcc gtaaaaatag tgaattggct
     1801 gatatccgtt tccgtcattt gttcagaaaa aaggtctttg atcttttctt taagatttcc
     1861 tgaaccgata cgcagaatat gatcaaaagc aggaaattcg ccatgtatat cactcaaaaa
     1921 taactctgtt ccttttggaa gctgcaaaat tgcttcgaga ttgatgattt ccgtgatgac
     1981 atcctccgtt gaggggaatt tttctgaaag taaatctaaa taacggtcca ttaaaacgcc
     2041 tccaaaaatt ggtatactca atattataaa gttgttatac actattttca atgaatatat
     2101 atccaatata gtaaaataaa taaaataggt ctatacaaaa agaagctggg aacaatagat
     2161 gtccagcttc cttctgtatt ttaggattgt tttatgcttt ttttcgttca ttcctcataa
     2221 ttgaaatcga ttgtttcaat gaactccata atccgccttc gctataaacg agtacattcg
     2281 atttgtacat gtttgcagac aagatcatca ataagaatgt tacggctaca agtaccaaca
     2341 aggaaatcaa tgctccaccc ataccgactg tctcatttgc taagcgaatc ggcataatat
     2401 atgaagataa gaatggaata tacgaagtga tccgaataac gatattgttt ggatctgaag
     2461 ctcctagtat caatccaagc atgtatccgc ctagagatag ataagtcact ggcaaaattg
     2521 cttttgctgt atcttctgct ttgttgacca aagagccaca taaagctgct aaaacagaaa
     2581 aaatcaaaat gccaaagatc atgaatagaa gagaatacca caagaatggt ccaatcaagt
     2641 tatccaatga gataccgtct aatatatttt tgaccatttc catatctttg aattgactgt
     2701 atcctatacc aaaaataata ccgtataatg ccatttgcgt taatgcaaca agtaaaacac
     2761 ctgttaattt tccgtaaaaa tgcttttgtg cagtcgtact ggacaggatc acttccatga
     2821 tccgcgtgcc tttttcagaa gcgatctctt gtgcaatgat ctgcgcatac gtcaaaataa
     2881 taatgaacaa gacaatcgtt gctacataac ttacgacgta ttggactgtc gagttatctt
     2941 ctccgactgt catttttcct tttgaatcaa aattgacttt ttgtcgagtc aaacttgctt
     3001 gttggctcaa gctagctact tcttctggtg aaatgcctaa ttgactcgct cgaagcatcg
     3061 attgcaagcc agtcagttgt tgttgtatca gtagttgtgt actttgtcct aatgaatttt
     3121 cgctataaag catcccattt atcttatcat tctcagttga gactactaaa tacgcatcga
     3181 ttttttcttc cgaaagctct ttttgtgctt tttcttcaga atcaactgct ttgaactcaa
     3241 aatcatctgt tttgacttgg ctcatttgct gactgatttg ctcattatca gccaatacgc
     3301 cgattttatt gacatcacta ttttgttgag caaagtttcc tgctacataa atgataccca
     3361 gcatgataaa agggactaat atcatgatca agaaagaaat cgacttaaca ttttttaaat
     3421 agacatcttt ggtaataatc caaaacttac gcatcgattt cacctgcttt caatttgaaa
     3481 atctcttcca aagttggcgg ttgctggtta aacataggaa tgtagccgaa ctgagtcgca
     3541 cgagtaaaga tttctttccc agcttcagga tcttctaagg taatctctaa cgtattgtct
     3601 tcatgaagga tcactttttt cacgccttcg atttcttcta cttcctgttg gcttaaacca
     3661 gattccaaga ataacttcgt acggccaaat gattcacgaa tctcatgtac ttttccattc
     3721 aaaaccattt ttccgtttcg caacatgatc aaatgatcgc agattttttc tacattatcc
     3781 atattgtggc tagaaaaaat gacacatgaa ccatgttttt tcaattccaa tattccatct
     3841 tttagcaatt cagcatttac cggatctaac ccgctgaatg gctcatccaa aatgatcaat
     3901 ttaggttcat ggattaacgt ggcgatcagc tggacttttt gctgatttcc tttagacaaa
     3961 gattttactt tatccgtctt tttccctttt acctgaaatt tttccatcca aaagtcgatt
     4021 tttggttcaa tctcttttct tgatttccct ctaagttctg caaaataaag cagttggtct
     4081 tgaatcgtta ctttcggata aagtccccgt tcctccggaa gataaccgat gacatcataa
     4141 tctttctccc caagatgatg gccattccat aacacttctc cttggtcttg tgtcaaaaaa
     4201 tccaagatca aacgaaaagt cgttgtcttc ccagccccgt tttggccaat tagccccatg
     4261 atttttccat cggggatttg gaaagagaca tgatcgacag ctgtgtagtt cccaaaagtt
     4321 ttaacaagat ccttgacttc taacatttct tcactccttc ttcaagaaat cacatatatc
     4381 cttagacacc agacgtaagc gatttattat ttaccaacaa aacgtaaacg ctctttttgt
     4441 tgtacataaa atgataggac actttacttt ctttgtaaag tgccctttcc tcattcctga
     4501 tgtaatttat caaatacgtt ttgtgcgaca tttttcggca gtccgatttc ttgtaattct
     4561 tctgtcgtgg ctgctgtgat atttttcaac gatttgaact ctttaagcag catttttttt
     4621 cgttttggtc ctaacccttc aatttcatct aatcgggaag caaaactgtt tttactgcgt
     4681 agctgccgat ggaaagtgat tgcgaatcga tggacttcat cttggattcg ctggagtaag
     4741 aaaaactctg gtgaatttcg ctcaagcggt actacagcca aatctgtacc gaatagcaac
     4801 tcatttgttt tatgtttatc gttttttacc atgccagcaa taggaatatc gattcctaat
     4861 tgattttcta agacatcttt tgccacatct acttgtcctt ttcctccatc gatcaagatc
     4921 aaatcaggta aaggcaaccc ttcgcgaatc actcgcgaat atctgcgata gatgacttct
     4981 ctcattgatg cataatcatc cggtccgttg actgttttta ttttatattt ccgatattct
     5041 tttcttgccg gttttccatc aatgaatacg accatcgcag ccacaggatt cgtccccatg
     5101 atattagagt tatcaaaaga ctcgatacgt attggggtcg ggatattcat cgcgtttcct
     5161 aaacgttcaa ctgctccgat cgtccgttcc tgttttctgg caatcagatc aaaccgttca
     5221 ttcaaagcaa ctgaggcatt cttactcgcc aattcaacta attttttctt ttctcctcgt
     5281 tttggttgca gcactttcgt agctaacaaa gcttctacac tgggcttgtc gatcgtatca
     5341 ggaatcaaga cttctttagg aatgaaatgc tcattttcct gataaaactg accgaggtaa
     5401 gtcaagaaat cttcttctgc ttcgttatag aaaggaaaca ttgaaacatc tcgttcaatt
     5461 agctttcctt gtcttacgaa aaacacttgg acacacatcc atcctttatc aatcgcataa
     5521 ccaaatacat ctcgatccag taaatcagta tttgtcattt tttggcgagt catgattgtc
     5581 tcaatggcac ggatctggtc tcggtattct gcagcttttt caaactccat gttttcagca
     5641 gctttttcca ttttctgatg gatctctgac tcgatttttt cgtgtcctcc gtttaaaaag
     5701 tttttcactt catcaacgat tttcgtatag actttcggat ctacgtcgaa ataataagga
     5761 cacaggcatt gtcccaaatg atagtaaaga caaggctcct tcgtctgact aggtttgcat
     5821 tttctcagag gaaataatcg atcgaggatc ttctttgtct cattcgccgc accgacatcg
     5881 ggatatggtc caaaatacag tgctttgtct tttagtacct tacgagtaat caccaaacgg
     5941 ggatattttt cattcgttat cttaaggaaa ggataggttt tatcatcctt taacataatg
     6001 ttgtatttag gatcattttt ctttatcaga ttgatctcta agagcaatgc ttcgatattc
     6061 gattctgtaa cgatgtattc aaagtcttcg atttcactaa caagccgttc cgttttcgta
     6121 ttatggctac cagtaaaata tgagcggaca cgatttttta atattttggc tttgccgaca
     6181 taaatgatcg ttccattttt atctttcatc aagtaacagc cgggctgatc aggaagtaaa
     6241 gctaatttgt ttttgattcg ttcgttcatc tggtactccc cttcatattc aattaaagtt
     6301 attatagcat gtctgtcaaa aaaacaatcc agcctgacaa cgtaattata ctcaaaaaat
     6361 attaatttga ataaccttaa ctcaaccgca aaaaaaccga ggacggaact ggattcagtc
     6421 ccaccctcag cttatttttg ctgtaagttt ttataaatat tgatcgatca tttgacgtag
     6481 ttgttctttt gaatgaacac caacagcttt ttctactact tgaccatctt ttttcaacat
     6541 taatgttgga atgctcatga taccgaattg ttgaggagtt tctggatttt catccacatc
     6601 cattttagca attctgaatt cttcttcatc atattcttgt tccaactgat ccaaaatcgg
     6661 tgcttgcata cgacatggac cacaccaagt tgcccaaaaa tcaatcaata caagtccttt
     6721 atctgtttct tcattaaatg tcttatctgt aattacttgt gacatatcaa agacctccat
     6781 ttttaaaatc tatgactagt atagcatttc tatacttgga tagcgaacca tctgcttacc
     6841 tttagtaagc tttacttaaa tttaacaatt gtcgcaccat ttccgccttg gtttgccggt
     6901 gcaaactcaa agcttttgac gctccgatgg tttttaagat aatcagtaat tcccttgcga
     6961 agtgcgcctg ttccttttcc gtggacaata gtcacttgag gataaccagc tagaatggca
     7021 gaatccaaat attgatctac ctcacttagc gcttcttcgt atcgttttcc tcgaaggtcc
     7081 aattgattcg gcacgtgact actagattca gatcgtacag catggataac tcgttgtgtc
     7141 ggctcttttt gaggagcaac aggcgtcagc tcgtcttcgg acacattcat ttttaatata
     7201 ccgatttcta cttgccactg attcccgttt ttacgtaaaa gcgtccctct ttggccgtaa
     7261 gtcgtaacca acacttcgtc tcccggtttc aacgtttttt gttctttcgc cttcttcaat
     7321 actttatttt tcttcaaagt ttcttcttga tgtagatttg ctaattgggt ctttgcatcg
     7381 atcaactgat gctctttcac atttccttgc ccaatctgct gttgcatttt acgaatatca
     7441 gcaatgattt tttctgcttt ttcttccgcc tctgacacga cttcatttgc ttttttcttt
     7501 gctttttcca tttctttttc acgttcttca aagaaatagc tgtaagcttc tttcaaatca
     7561 tcgtgaagtt cttgtgcctc agaaacaaaa tgacgcattt ccagatattc ggtctctgcc
     7621 attttccggc gattctctaa atctgtgatc atttcgttta aatcttggct ctcatcattc
     7681 atcaactgct tcgcttcatc aataacttct gtatcaagac ctaatcggct agagatttca
     7741 aacgcattgc ttcgtccagg aacgccaatc agcaaacgat acgttggact taatgtatcc
     7801 acatcaaact ccatgctggc attaatcgta tttgcccgat tatatccata aactttcaat
     7861 tcaggataat gcgttgttgc catcacataa gcagactttt taccaagatc atctaagatt
     7921 gcgattgcta gagcagcacc ttcttgcgga tctgttcctg caccgagctc gtcaaataat
     7981 accagacttt tttcattgac ttttgataaa atatctacag tgttcgtcat atgagaagaa
     8041 aaggtcgata gactttgctc aatcgactgt tcgtctccta tatccgcaaa tacttcctca
     8101 aaaataccga tttgactttc ttcacctgca ggaatcggta atcctgcttg ccccatcagt
     8161 tgtaacagac ctaaagtctt caacgtgatc gttttcccac ctgtgttagg tcctgtaatg
     8221 acgatggctt gatagtcttt tccgattgtg atgtcattag aaactacttt ttcctgatcg
     8281 atcagtggat gtcttgcttg tttcaagacg acatgattgt cttcactgat tcccggcacg
     8341 atggctttta cttctttacc aaatcgtgct tttgcattca tcagatccat tttaccaatc
     8401 acataagtat tgtggatgat ttcttgacga tggggaacaa gttcagcaga aagttcactt
     8461 aaaatacgtt gaatctcatt tcgttcagcg atttggtatt gacgcaaacg attattcaaa
     8521 tccaccactt gttttggttc aataaataaa gtctgacctg aagcactttg atcgtggact
     8581 acaccgccaa atacaccgcg gtattcttgt ttgactggga taacgtagcg gtcatttcgc
     8641 atggtgatga tcgcatcact taaatacttt gcatttttcc cacggacgat cccatctaac
     8701 tgttcacgaa cagcttgttc acttcgccga atgttctgtc ttatgctctt taactcagga
     8761 gaagcgtcat cagtcacgcg tccgtcttca tcgattgctt cctttaaacg gcggctcaaa
     8821 acagggatcg tgactaactg atctgcccac atatacaaac gtgcaaattc gatttcactg
     8881 tcagaaagat catcaatgaa acgattcaat tcgcttgtcg tagacaatac tctggaaact
     8941 tgtgcaagct ccactccatt caagtctgct ccgatctcaa tccgtttcat atggggacgg
     9001 atattttcaa ttttaggtat agggatcccg cctcgcagac gttgcacttt caatccatcc
     9061 tcagtttctt gaagccagtt tgtgatcgtt tgtttctcac tcactggtac caattctgcc
     9121 agttcttctt ttccttgtgc agtcactaca aattgtctga ccatctgttt gactttttca
     9181 aattccaatg tttcaaggat tcggttattc attttatcac cttatctttc actatgaaaa
     9241 aagagctgtg ccggacatac gctacgcagc cttcttaaat aaactactct gtacaaaaaa
     9301 cgaaatattt tccagcacta ttttccttaa ttcaaaatat tcgttaccca aagctcatac
     9361 atcttgttgg taagaaatgg cgttctctca acgataaaac gagcgaaact atgctcgccg
     9421 aattgatttt ggaccacatc taatggaaca aaggcaagta actgcaaaat caaaaatata
     9481 gtaacgtaag ccatgattag tgacaggatg cctcctgcaa tccagtctac ctgtctcaag
     9541 atcggcacat acgtcaatcc atggacaaaa acccccacaa atcgggtaag cagccagcca
     9601 attagcaaaa taatcagaaa agcagttcct gcataaaaag cttcatctag gtgaaaggca
     9661 aatacctgat caaaaaatgc taactttgat gttggggtga cagctgggta tggaatgtat
     9721 aattccaaat gacttgctaa tggtttgtaa aaatgctggg cagcaataaa tgacaataca
     9781 tatccaatcg cgaatatgat ttgcaaagca aacccgcgac gtgctccaga aaaaaacgca
     9841 attagcaata aaaataaaat cagcaaactc agcatgctac gttcctttct cttctaactt
     9901 ttttaaattt ttcttatttt gataaaagga tcttaattag aagatttttg tttaatttgt
     9961 ccttttcgtt tctcgtttaa aatctgttgt gcttccacat gatttttaat ggtatgatct
    10021 gcttgaccat tttctttcaa tacttcccga gcttcgttct cgattgcttc aatgcgtttc
    10081 acacgatttt caagctctgt aaatttgatc atttttttct ttaattctgc tgtttcttct
    10141 tccagctcca atattctttc ttgtttcttc aattgatccg aaagcgcatt gacagccatc
    10201 aagattgctg cttgttcatt atccatttgt ggtgataatt gtttcaattc tgctaactgt
    10261 tcattgatca gcttcgttac gatatccata tgatgcttgg tttcccgtcc gataatagta
    10321 tatgtttggt tggcaatcac cgctttgtag cgtgtttttt cgtgcgccat acgaatcatt
    10381 tcctcctatt tgatgaaagt tttatttagt gtaccatgaa gttggttctt tcaccgttct
    10441 ccacaaatat ttttcacata cttaactatt atatgataaa ctttaaaaaa atgctatgtc
    10501 tcgcaatctc ttagaaaacg aaaagtcgtg agttgcggta tagttttatg tataaaaacg
    10561 aggagaattt tatgagcaac gtcattatca aagcaacaaa cgaccaactt agtcaaatga
    10621 aagcatacta tcgctcttat ttgttaaata aacagattcc ttatactagc tttgctgcaa
    10681 aaaaagacgg gacgaccatt accgcttata catccggaaa agtgatgttt caaggtacga
    10741 atgcagcaat agaagctgat aaatgggggg aggcacagga taaacaagcc aagccagcaa
    10801 aaacagcctc ttctttgcca aaaaatatca gtcaattatc cgttttaggt agtgatgagg
    10861 tcggaaatgg cagctatttc ggtccgctaa cagtatgtgc tgcttatgtg aaacgtgatc
    10921 agcttgcaga attacgtaaa cttggtgtta gagattcaaa agagttaaaa gatagccaaa
    10981 tcatccagct tgctgacatc ttaaaaaaaa ccatccccta taaactgctt gtcttatctc
    11041 cagaaaaata caatgagatc caaccaaaat acaatgccgt tcggatgaaa gtcgctttgc
    11101 acaatcaagc gatccatttg ctgctaaaaa aaatcgagcc agaaaaacca gaagcgattt
    11161 tgatcgatca gtttacgccg gaagcaaatt ttaaaaaata tgcgcgttta gaaaaaaatc
    11221 aattacaaga aaaactgtac ttcgttacta aaggagaaca atatcattta gctgttgctg
    11281 ctgcttctat catcagtcgt gccagctttt tggaagagtt agacaaagca tcagctgaag
    11341 ctggaatcac ccttccgtca ggagctggaa cgaaatctga ccaagtagcg gcaaaactat
    11401 tggagaaagg cggcatgcct atgcttgaaa aatatgccaa acttcatttt gccaatacag
    11461 aaaaagcttt gaagctacta aaaaaataat ggaactgtga caaaagtcat gtcacagtcc
    11521 cttgttccga ataaacggtg ttcaatcatc tga
//
LOCUS       DACEMO010000083        11434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEMO010000083 DACEMO010000000
VERSION     DACEMO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.085"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(133..306)
                     /locus_tag="IW728_001628"
     CDS             complement(133..306)
                     /locus_tag="IW728_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1024990.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IW728_001629"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IW728_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IW728_001630"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IW728_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1024991.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IW728_001631"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IW728_001631"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1024992.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IW728_001632"
     CDS             5704..6489
                     /locus_tag="IW728_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1024993.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IW728_001633"
     CDS             6502..7353
                     /locus_tag="IW728_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1024994.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IW728_001634"
     CDS             complement(7477..7929)
                     /locus_tag="IW728_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1024995.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="IW728_001635"
     CDS             complement(8394..8762)
                     /locus_tag="IW728_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1024996.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="IW728_001636"
     CDS             8912..9394
                     /locus_tag="IW728_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1024997.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="IW728_001637"
     CDS             9431..10441
                     /locus_tag="IW728_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1024998.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="IW728_001638"
     CDS             complement(10556..11269)
                     /locus_tag="IW728_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1024999.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         3860 a         1905 c         2361 g         3308 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataa
//
LOCUS       DACEMO010000084        11424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEMO010000084 DACEMO010000000
VERSION     DACEMO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.086"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1522)
                     /locus_tag="IW728_001639"
     CDS             complement(<1..1522)
                     /locus_tag="IW728_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1025000.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDA"
     gene            complement(1704..4097)
                     /locus_tag="IW728_001640"
     CDS             complement(1704..4097)
                     /locus_tag="IW728_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1025001.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(4204..5064)
                     /locus_tag="IW728_001641"
     CDS             complement(4204..5064)
                     /locus_tag="IW728_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1025002.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(5123..5644)
                     /locus_tag="IW728_001642"
     CDS             complement(5123..5644)
                     /locus_tag="IW728_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1025003.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            5964..6683
                     /locus_tag="IW728_001643"
     CDS             5964..6683
                     /locus_tag="IW728_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1025004.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            6709..9300
                     /gene="mprF"
                     /locus_tag="IW728_001644"
     CDS             6709..9300
                     /gene="mprF"
                     /locus_tag="IW728_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1025005.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(9488..10675)
                     /gene="tuf"
                     /locus_tag="IW728_001645"
     CDS             complement(9488..10675)
                     /gene="tuf"
                     /locus_tag="IW728_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1025006.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            11043..11399
                     /locus_tag="IW728_001646"
     CDS             11043..11399
                     /locus_tag="IW728_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1025007.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3340 a         2372 c         2030 g         3682 t
ORIGIN      
        1 atgcatcatg aataaactga aattctcgtt cttttaaaat attacggaca gaaacttctt
       61 taaatgcacg atataataga atctgctctg ccttttctac ttgcgtcaaa ggtctctttt
      121 tccgtgcagt ttcttgtctc gtaacctgag tgtgttgact ttcctgaggt tcttgacgat
      181 tttgctgatt tgtccttcga agatcgcgca gttgtttttg tatcgtctct ctgccgatct
      241 gaaactctgt tgacagttga gatagataca tatcttgttc gattggtgaa acaactcggc
      301 taagttcaac tagtaattct tcgaggtatt cgatcttgtc tttttcatta tctaaatttt
      361 tcccttgttt taaataacgt gatttaaaag taaagactgt ttcgcgccca tgagccacta
      421 attcttggaa agatgcttct ccatacttac ggagatattc atcaggatca agcttttctg
      481 gaacgactac gacagaaagt gctaatcgac tgttttgccg aagcaattca atccctcgat
      541 tggttgcttc aaaacctgcg ttgtctccgt catagcaaaa gaccaattct ttcgtcagct
      601 tttccatttg ttggatctgt tgagctgtta gacttgtccc catagaagct aatccagttt
      661 tgataccagc ctgccatgca gcaatcacat ccataaaccc ttcgaacaac aaaatttctc
      721 cgtttttccg gatatcgctt cttgctttat caagattaaa taaaacttgt cttttattga
      781 acagttctgt ttctggacta ttcaagtatt tgggctgatc tttttcctgc cctttttcag
      841 gtgcaagcca gcgtcctgaa aaaccgatcg tctggccttg agaattacga agtggaaaca
      901 tcactctttg ataaaaacga tcagacatac ggccatcatc atgcgtgaca aacaatccag
      961 agcgttctaa caattcattt gaaattgatt cattcttgaa tacttgctga agaaaagaac
     1021 gctcattagg tgcaaaacct atgttgaatt cttcaattaa ttctaaagac aacccacgac
     1081 tttgcaaata ttctaaagct ggctgtccag cttttgtatg caatagcatg tgatgataga
     1141 tttccgctgc tttttcatga gcagcaatca gtttccccgt agcagattca ggattttgga
     1201 cagctatagt acgctgacgc cattgatctt ctaacggtat ctgttcgaag tctgccactt
     1261 ttaccactgc ttctggaaaa cttagacctt ctagttcttg aagaaaagta aacacgtttc
     1321 cgcctttacc acaaccaaag caatgaaaaa tctgcttgtc ttctgcaaca gaaaaagacg
     1381 gtgtacgttc ttcatgaaaa ggacacagcc ccaagtaatt tttaccagac tttttcaact
     1441 ggacatattg tccaatcacc tcaacgatat tcgtttgact gcgtactgtt tcgataacct
     1501 cttgaggaat ccgttgagcc attttttcac cccctgctct attgatatct tttgggttta
     1561 acagaagata tttcaggaga ataaccggta aaaaatcgca ctaactgaaa tcagtgcgat
     1621 tttaaaggac acaattatac atgtttgatt gtatcatatt tcattgcttt ttcaagtata
     1681 acacatttct atctcattaa gcatcaattt gctactcttt tcagtaactg attttgaata
     1741 tcagccttcc agtacaatac tcgaagaaca atatttacga gcatgaatac aaagaagtat
     1801 cctacgctct ctcccaaccc taagtttact tccaaaattc catggaacag cattgcagct
     1861 acatatatcc ctacgatcgt cgttactgct aggaagatgc gcaatgggtt gaagggataa
     1921 caagctttta caactgccat gcaactgatc ccaatcagca agtaatacat caaagtagta
     1981 gtttcagcat ttccccatgc gaatgttttt ccaagtagat acaccgcaat gatattcact
     2041 actacaagca aggcatttgg taaagcatta ataagtgctg taggcaaata acggtaagtg
     2101 atttttcttt tatcttgttc aaaagataaa aagaaggaag gatacccttc aatcgctaaa
     2161 tcaatcagcg tgatttggat agggataaac ggaaatccaa ttgctgtaac ggcacatata
     2221 atcgataaaa taaatgaata aatcgttttg ataaaaaaga tgcctgagac tttcgtcaca
     2281 ttgttgacca ctcggcgtcc ttcaaacagg acttctggca atgtagtaaa atcagagtcc
     2341 aacaatacca gattcgcaat ctgacgtgtt gctccatcac cttctgccat agcaatgctg
     2401 caatctgctt ctcttaacgc caaaacatca ttcacaccgt cgcctgtcat tgcaaccgtt
     2461 ctgccgcttt ctttcaattc atttaccagc aatttctttt gctgcggact cactcgtcca
     2521 aaaaccgtat agcgatgagc agcttctcgc acttccattt cttcagtaat ttgagaaaga
     2581 tcgacatacg catcataatt cggtaatcct gcacgtctag cgatattgga gacagtaaaa
     2641 ggattgtctc cagaaatcac tttcagatcc acaccttcct catgaagata agctagtgtt
     2701 tcttgagcgt tttttcggat tggatcatcg atttcaaaca gagcaaccgg ctcaacaaat
     2761 gctggttttt cttcgttcaa ttgctgattt ggagcgatcc ccaacataag tacgcgatag
     2821 ccattcgctt gggctgtttt tagactgtcc ggaagccgat cttcttccac caaacgttca
     2881 ggcgctccta cgtacacgac tcccagttct ggaaattcca tcgcgcccca tttgcgatca
     2941 gaagaaaatg cgactttgtt tgtcacatca taccgattag aaagctcaaa ataatctctc
     3001 atagcccgca tagtgatgtt gttatcactg ctttctgcca gatacgtccc cataatttct
     3061 gggatggctt tctcgtacag cggatgtagt gcttctaccc gctggatact cattttccct
     3121 tcagtaatgg tccctgtttt atccaagcat aatgtatcga catgggccag cgtttcaatt
     3181 gaatacatgt cttgaacaag gattcgtttt tttgccagtt tggttacccc tgtcgttaat
     3241 gcaatactta ttagcaatac caaacccttt ggaagcatgc ctaacaaagc tgctgcagat
     3301 gcaataacag aaattttgat agaagcatcg cggatgaaca gtgcttccaa aaatagtatg
     3361 atccccaaag gaataatcac gaaactagtg aatttggata ctttgcgaat cgaatctaca
     3421 agttcagaac gaatagcttt gtgagtcttt gcctcgctag tcaagcggac agcataatta
     3481 tctgcaccta ctcggataac ttcagccatg acagaaccgc ttgtcaaaaa gcttccagaa
     3541 agcaattcag ctccagtttc tttgacaatc aaatcagatt cgcctgtcaa aagagcttca
     3601 tttgcttctg cccgtccaga tataacctgt aaatcggctg gtatctgttc tcctgctccg
     3661 atttttacca aatctcctaa taccagctct ttagtagaaa tcgattgttc tttaccgtcc
     3721 cggatcacta gtacttctga ttgggaaaca atcgataatt tccgtacaag atttcttgca
     3781 tgtatttctt gataaattcc aataacgata ttgacgacaa taattgctaa atacgccatg
     3841 ttggaatacg cacctacaaa taacagacaa atcccaatac caaagttcag aaaattgaat
     3901 aacgtcaata tattatcgct gaatatttgt tttgtacttt ttgatgcatc ttcctggtag
     3961 tcattttgct ggccagaagc cattttctga actacctctt gctgtgtaag tccattcatt
     4021 ttctttttct ccattttccg ccaactttca aaaataatca cgtgtttaag tctagcgtgt
     4081 tccaagatta aaaacaactt gtttttataa aatttataga aaaacacacg aatgatttta
     4141 caaaaaagat gccaaccttt ttgtttgaat cacccgtgtg ctgtttttat tatcaatcta
     4201 tctttactta tattgttcct ccaaacggct catccaccaa cctagtgctg taccggctat
     4261 aaaaagaaca ggacaagcta gtaccccagc aactcccaat cccgcgtaat cggtaggaat
     4321 gatcataatc gttgaagcaa aaactacacc taaaatgaaa tggaatagct tgctatatgc
     4381 ctttgagaaa atgtaatcca tgatttttga caatccaaga acacaaagaa tcccaccaat
     4441 tccgatcggc acaatcactt caagtcgtac ttctttaaat ccgtcagcca tcgctttata
     4501 caatcccata tacaccaaaa aattagaagg gctcaatcct gggacgatca tacccaaacc
     4561 aatcaatccg ccggcaatca gccatgttcc aaaattctga tcgacttgcc cgtcaaataa
     4621 ttgagcgccg tataataaaa acaaataagc cagaacaaaa gtaacaacca tgatgatcac
     4681 atcagaagta tctcttcctt ttttccctgc ttctttccat aacgcaggaa cagttccagc
     4741 gatacatccg acaaaaaacc ataatatcgt tgtttcatac gcacctaata aaaaactgac
     4801 taaaaaagaa agtagaaaga tgcctgtcac tccgcctaga ccaacaggca gaaaaaacaa
     4861 aacattttct ttgaaatttt ttgtaaggtg ggctaaaaaa gaaatcagcc gctcatacat
     4921 gccaaagaca gctgctaatg caccaccgct gactcctggc aagataaatc ctgaaccgat
     4981 aaacattccc ttcacaaacc gtaacatcca gtctttggtt gtgtacccca cttcttgatt
     5041 ttccattttt tgcctttttt ccactatttg cgcactcctt ctttcattta attttattca
     5101 tgattgaaca tttcctgcta ttttacttaa agactttttc ataggtctct attatttttt
     5161 cattcatttt gacaatatca ggataaaaca tgttacctcc attatgatag agatagccgc
     5221 cattgtaaag caaggactgg atccgccaat atcggtattg tttcccttcc tcatttccta
     5281 aaagtgggct cagcacatct ttagaatacg cttctgctaa ttctactgtg ttttctccgc
     5341 catgattttt tacataggca atataatctt tcccaaaatt atatgcttgg acagctgtcc
     5401 ataaatcgca tccttgtttc tttgcttcta ctatcatttc cgccaaatat ttgacacctt
     5461 gatcaatact gtcagctgga ttatcaatga tctcagcttc tccatgcaga ctttccgagc
     5521 tctgcatgag atctgtccct tttccttttg attcggtcaa tattatcgcc ttgatcaaat
     5581 tctcgtagtt ttccatatga taattttttg cagctgttgt tatttctatt tgatagcttt
     5641 ccactcgttt taccgaacga taagttcttc ctcataaaaa caacaccgca ccaataaccg
     5701 tcagaatgat catgtaaaaa agaatacgga taatagataa tcgttttcgc ttcatttcat
     5761 cacctagtta ttttgacatc tttttttatc ttactatttc tttatcttct tggctatttc
     5821 ttccttgatt tctgtgtacg ccttaagaaa aaatgaattt tcctgtattt tttgacatct
     5881 tacgaaaatg atcgttgttt ttcagtaaaa acaattgccc aatgactgat ataatcgtta
     5941 tactttacac agtaggtgat tatgtgaaaa aagaaaagaa atatgcaaaa atgccagatg
     6001 ggagcgaaat ttactatgaa aagagcggtc aaggcttccc tctcttttta ctgcatggca
     6061 atgacggcag cggccgcttt ttttcagaac aggtccctgt actcgaacgc tactataccg
     6121 tgtatctagt agatagccga ggacatggtc gttcgacaaa cgaagcatct atgttgaatt
     6181 ttcagttaat ggccgaggat ctgaatacga tcatgttgct ggaaaaaatc gatcaggcag
     6241 attttcttgg atttagtgat ggagcaaatc tagcactggt atttgccagc agcttcccta
     6301 aaaaagttca tcgattgatt ttaaattctg gaaacacgct tgttaaaggc gttcgttttt
     6361 ctgccagagt catcagtaat attcattatg catgggtatg gttactctct ttatttcgcc
     6421 caagtcttcg caaaaatctt ttagttatca aacttttgct gcatgacatc gggcttacag
     6481 aaaatgattt aaaaaaaatc aactccccca ctttgatcat cgtgggaaaa aaagatgtaa
     6541 tcaagctaaa acattctttg tatatagcta aaactatacc aaaagcttcc tttgtgcttg
     6601 tgaaagaaca aggacatgaa ttagctagaa aagatcctga acgcttcaat cgagaagttc
     6661 ttcaatttct ttctgaaaca taactattag gaatagaagg tgatttattt gttaaaaaat
     6721 accatacaat ggctgaaaaa ccacctagga ctgttcaaaa caatcttttt gatttctgtc
     6781 attgtcatta ttgtcggtga attgatgtcc atcggtaaaa ctctatctat ccagcaatta
     6841 ggagaaacgt ttgccactat acctgtatgg aaaagcggac taatgctatt gattggtctt
     6901 atctctgtgt tacctatggt tggttatgat attattttga atcggatgct tgaacaaaag
     6961 cagaatcctc gttacctgtt tgagacaagc tggctgatca atacgatcaa caatattgcc
     7021 ggtttcggcg ggtttgttag tgtggggctt cgctctgaac tatatggaca aaagaaagag
     7081 agcagaaacg tgatccaagc attatcgaaa gttttcttgt ttttgatggc tggactttct
     7141 atttacagtc tgctttcttt tttacttgta gtcttcacgc cagttgctcc ttttttgaag
     7201 caatattgga tatggttgat cggcggcggt ctatattttc ctgtcgtctt tttatttact
     7261 actttcaaaa agaaaggatt ccttggtggt acctcactta aaactcaagg acagctattg
     7321 ctagtttcat tgttggaatg gaccggtgtc ctgtttagtt tcctttctgt tggttattta
     7381 atggaaatcc ggattgatct gtggcaaaca attccattat tcattgctgc ttcagtgata
     7441 ggaatcgtgt ctatgattcc aggagaaatt gggagtttcg atgtaatgat gattatcggg
     7501 ttgtccgcta ttggtgtgcc aagagaaata gtcgttgtgt ggatcttgct ctatcgcctg
     7561 ttctactaca tcattccgtt tttgattgga atcgtctttt tcttcaaaaa tatcggttcc
     7621 acttttgatc agcgttattc tggtatccct aaacaattag caacagaaat tgcgcataaa
     7681 atcgtggttg tcttattata cttttctgga atcatgctag tactttctgc cactatcccg
     7741 caagctttta cagagtttcg ctggcttcat agtttgaacc cattgaaatt tcattttatt
     7801 atccaatttc ctagtatact attgggattt cttttaatca ttatgggcag agggatcgca
     7861 gcacgtgtga aacgagcata tttgcctaca atcttcttga ttgttctagc tttactttat
     7921 gttttattaa gtgattttag tttcacaccg gtggttttct tgtctattct gcttctgatc
     7981 attcttgctt ctaaaaacga attatttcgg gaacaattga tctattcatg ggagtggcgt
     8041 acgatcgatg gaatcattat tggtgcactg tccttgcttt acatcgttat tggggtatac
     8101 aatctgccgg atttccctca tcggcgtcac cattttattt cctttttctt atttccttct
     8161 gaaaagattt ggttttcggg attgttggcc atcatcgctg ttagtttcat gatcgttcta
     8221 tttgttcatt tccttcaagg agaaaagaaa cagattggcg aagcctttaa tgaagaaaag
     8281 gccctgaaga tccttacaac ttatggagga aactccgata gtcaattgat cttcttaaaa
     8341 gataagcgga tgtttgccta tgaaaaagac ggtgaaccaa ctgttcttct ccaattcgct
     8401 tgcttcaata ataaatgtat tgtaatgggt gatccttctg gtaagaaaga agattttcca
     8461 gaagcaatcg aagcttttat cgaagaaaca gatcgactat gctatttacc agttttctac
     8521 gaaacaagcg aagagattgt tatgattttg catgaattcg gctacgattt cattaagatg
     8581 ggggaagaag cttatgtaga cctgaatagt tttacaactt cagggaaaaa aatgaagggc
     8641 actcgtgctg tcttgaatcg gatcgaacga gaaggattta catttgatgt cttacaacca
     8701 ccattttcta ctgagcaaat gagtactttc aaaaatatct ctgataattg gttgggttca
     8761 cgaaaggaaa aagggttttc tttaggcttt ttctctgaag attatctaca aagagcgcct
     8821 attgcagttg tcaaaaatac ccaagaagaa attgtcgctt ttgcaacgat catgcctact
     8881 tacacgaaca ataaagtagg aacgattgat ctgatgcgat atgattctaa aaaagcacct
     8941 tctggcagta tggatttctt gttcttgcat ttgtttgcct atatgaaaga agaacatatc
     9001 caatggttca atttagggat ggcccctctt tcaaatgtcg gaacttccag aaagagtttt
     9061 ttacaggaaa ggattgcata cttggtttac gagtttggtt ctcattttta ttctttccat
     9121 ggattaagag aatataaaag caagtacgct acaaactggg tctcacgtta tactctatat
     9181 tctagagata gttggattgc atatgttatg attgctttgc tgattattga taatgcgcca
     9241 gttgagcaga cacaaggtag aataagtggt ttgaaaaaat ggatacgaag aaagtattaa
     9301 aaactgcata ccgatagatg aacactctca tgatgaataa aagagactga agtaaaacga
     9361 aagttcatgc ttctacaatc tcttcttata caattgaaga acaaagagcg gtccaaacag
     9421 aaatcctgtt tggaccgctc ttttatttta aacatttttc attaccaaaa tattcgagtc
     9481 gcgtttctta tgcttcgatc tctgtaacga tacctgaacc tactgtccgt ccgccttcac
     9541 gaattgagaa tgtcgttccg ttttcaacag cgattggatg gatcaagtca acatcaatcg
     9601 tcacgttgtc accaggcatg accatttctg tatcttctgg caatgtgatt gttcctgtaa
     9661 catcagttgt gcggaagtag aactgtggac gatagttgtt gaagaatgga gtatgacgtc
     9721 caccttcttc ttttgtcaac acgtacactt ctgctttgaa ttttgtatgt ggagtaatgg
     9781 aacctggttt agcaagtact tgtccacgtt cgatatcgtc acgttggatc ccccgtaaca
     9841 acacgcctac gttatcccca gcttccccgt aatctaacgt tttacggaac atttctacac
     9901 ctgttacaac tgctttttgt gtttcaggtt tgatccctac gatctctacc tcatcaccga
     9961 cacgaacagc accacgatca atacgacctg aggcaacagt tcctcgacct gtaattgaga
    10021 agacgtcttc cactggcaat agtaatggtt tatctgtatc acgttctggt gttgggatat
    10081 attcatctac tgtatccatc aattccataa tagcagcttc agcatctgga tcgccttgca
    10141 atgcttttaa tgcggaacct ttgatcacag gagtgtcatc gcctgggaaa ccatattcgc
    10201 tcaataactc gcgaacttcc atttctacca aatcgatcaa ttcttcatca tcgactaaat
    10261 caactttgtt caagaaaaca atcaggtatt ttacaccaac ttggcgtgac aataaaatgt
    10321 gttcacgtgt ttgaggcatc ggaccgtcag ttgcagaaac aactaagatt gcaccatcca
    10381 tttgtgctgc ccctgtaatc atgtttttca catagtccgc gtgtcctggt gcatcaatat
    10441 gcgcgtagtg gcgtttttct gtttcatatt ctacgtgtgc ggtattgatc gtaatcccac
    10501 gttcacgttc ttctggagct gcatcaatac tagcatagtc ttgaggattt gctaggcctt
    10561 ttttgcctaa tactgtagtg atcgcagcag ttaaagtagt tttcccatgg tcaacgtgtc
    10621 caatcgtacc aatgttaacg tgtggtttac ttctgtcata atgttctttt gccataattt
    10681 acaaacctcc taaataatat agatcactga aaaatttcag ctctcacctt tttattatag
    10741 tctttttttg actaaatcaa aaagaatacg tccgacttac tttttgtaag attcgaacta
    10801 agcttattat acgtcaatat tggtcaaagt caactaagag cactcgtctg tcttatacgc
    10861 gaaaaaagta ccttttataa ataaaaaaat gtgttttttg agaaaaactt caagatagtc
    10921 cttaactttc ttttgaagca ataagaacaa aatgaggaaa agtttaaaat tcagctgttg
    10981 ccaacaactt tcaatagacg aaatcttcta tttcgttaat aataaggaaa gaggtgaagt
    11041 acatgaaaaa attaattggc gtacttgtcg ttttaggcat atttctcggc ggaagttttg
    11101 cagcttatca ttatttttac ggcggcgaag cttattatac aaaaatcacg acgaatggcg
    11161 aaatgtcagc tacccaagca gataacggag agaaattcac gacttatacc tacaagcaag
    11221 cggcttataa taaagatggt gaaaaaaaac aggtgacgct tcgcgaagaa agagaacatc
    11281 cattaaagat gaatgcgtat cttaaactca aagtcaatcc tcgtaaaggt gtattaagct
    11341 gggaagaaat aaaggcttct gaagtgccta aaaatgcggc agaaaaaatc gataattagt
    11401 aaatggtctt aaaattttat taga
//
LOCUS       DACEMO010000085        11209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEMO010000085 DACEMO010000000
VERSION     DACEMO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.087"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..661)
                     /locus_tag="IW728_001647"
     CDS             complement(5..661)
                     /locus_tag="IW728_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1025008.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            854..1132
                     /locus_tag="IW728_001648"
     CDS             854..1132
                     /locus_tag="IW728_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025009.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1151..2359
                     /locus_tag="IW728_001649"
     CDS             1151..2359
                     /locus_tag="IW728_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1025010.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2507..2872)
                     /locus_tag="IW728_001650"
     CDS             complement(2507..2872)
                     /locus_tag="IW728_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1025011.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3019..3792)
                     /locus_tag="IW728_001651"
     CDS             complement(3019..3792)
                     /locus_tag="IW728_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1025012.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3966..4826)
                     /locus_tag="IW728_001652"
     CDS             complement(3966..4826)
                     /locus_tag="IW728_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1025013.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4826..6244)
                     /locus_tag="IW728_001653"
     CDS             complement(4826..6244)
                     /locus_tag="IW728_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1025014.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6301..6750)
                     /locus_tag="IW728_001654"
     CDS             complement(6301..6750)
                     /locus_tag="IW728_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1025015.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7195..7458)
                     /locus_tag="IW728_001655"
     CDS             complement(7195..7458)
                     /locus_tag="IW728_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1025016.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7557..8852)
                     /locus_tag="IW728_001656"
     CDS             complement(7557..8852)
                     /locus_tag="IW728_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1025017.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8849..10120)
                     /locus_tag="IW728_001657"
     CDS             complement(8849..10120)
                     /locus_tag="IW728_001657"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1025018.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10285..11154)
                     /locus_tag="IW728_001658"
     CDS             complement(10285..11154)
                     /locus_tag="IW728_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1025019.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT         3137 a         2365 c         1948 g         3759 t
ORIGIN      
        1 ttcatcaaaa gataatattg tcttctaaca agtccatatt caaaataacg atatgatggt
       61 ctttaacggt gataatacca tcttttttca gttgttttaa aattctgttt acactgctag
      121 cagttgagat gccacaaaat ttggcgattt cttcattcgt gatcgaaaaa tcgatcagca
      181 tcccttcttc tgtccgcaca ccgaataaat catataattc gtaaagttgc gtacaaacag
      241 ctcccagctt tccgtttaat agcatttgct gcattttttt catcgaataa agcagacgat
      301 agcggtaata ctcttttaca tagtgttgta gctttgggtc ttggttgata ttttgccaaa
      361 agcgcacacg attgattcga taaaactctg catattctga ttcaactcga atattatacg
      421 gcgaatcgat gaattgtgaa tattcgtctt ttaaaagaga gacgatttca agatctttga
      481 tatagcgaag gttaaattct ctcccatcat ttgaaataac acttgctttt accgttccct
      541 ctttcaagat atacgtatgg gaatcttcca tcccttcgaa tgtcaaatat ttatgatatc
      601 ctttgcgtat aatcggaaaa tcatttttat ccaaatattc ctttaagact ctactatcca
      661 atgatgctcc cccatccaaa ctttcatcta aaaagatcat acagtacttt aaaacttttt
      721 aaagtccaaa ggtattttac taatgatatt aacatttgtt aagtaccttt ttccatcact
      781 tatgcgattt ttttcctttt ttttattcgt tatactgcag aagtaaaatt ttttaagaat
      841 agagggaaaa aacatgaatt actggatgaa cacaatcatt aatcgattag aaacagcata
      901 tcaaacaaga tttgatatga aagcttcttt agtcttttta aatgatgcgt atcaaaactc
      961 gatcgaattg atcaaagcag tggatgagaa tccaacaaac gaatgcgaag aatttttaaa
     1021 tctgtttatg tctacaagag atttatttat tcgccaactt gttgatcgct atccatccaa
     1081 ttatcacgat gtagaagttc aaatacaaaa attaaaagca tattcagctt aaggggaatg
     1141 tataacatac atggaaaaga aattatttaa taaatatttt attggaatca cattgatcaa
     1201 ttttattgtc tacctgattt attatctgtt gatggtcatc atggcagtca ttgcgcaaga
     1261 caccttacac gccagcttga gccaagcagg ttttgcttct gggatctata tcatcggaac
     1321 attgctggca cgcttattca tggggaaaca gttagaacta tttggtcgga agtttacctt
     1381 aagaggcggc gcgatcttct atctcctttc aacattagca tatctttata cgccaactat
     1441 cggggcattg tatttagttc gattcttgaa tggattcggc tacggtacag tttctacagc
     1501 tacaaatgct atcgttactg catatatccc tgcatctaaa aaaggggaag gcatcaatta
     1561 ttatggatta agtactagtt tagctgccgc tgttgggcca tttttaggaa tgattttgct
     1621 gaatatgact gattttcgtt tcattatctg gttttcaatt gtactggtct tctttgttac
     1681 gattgcctgc cttgtctttc ctgtaaaaaa tattccatta tctgaagaac aaaaaatatc
     1741 tcttcgctca tggaatatca atagttttat tgaaaagaaa gcacttttca ttacaggaat
     1801 tgcctttttg atgggtcttt cttattcaag tgttttatca tttttatctt cttatacaaa
     1861 agtaatccac ttagttgctg ctgggtcatt tttcttcgtc gtatatgctt tggtcatcac
     1921 atttacacgt ccattgactg gaaggatttt cgatgtcaaa ggagaacaat acgtgatgta
     1981 tcctagctat ttattcttaa cagcaggatt atttttactg agtgtaacga cgaatagtgt
     2041 gacattgctt atctctggag cattagtcgg cctaggttat ggtacgttta tgtcaaatgg
     2101 acaggctgtt tgtttaaaaa tcgtccgcga acatcgaatc agtattgctt tatctaccta
     2161 ctttattggt ttagacctag gcttaggcgt tggtccttat ttgatgggga tgctaaaatc
     2221 tcttacttca tttagaggat tgtatgtgat tgccggagtc attccattga tatgtacagc
     2281 actctatctt gttaatggcc gaaaacatag tcatgaagca gagggcgaat tgattgaatc
     2341 attgaaaaat gaaatttgat cttgatgaat agataaaaaa aaccgttcag atctgaaaat
     2401 aatctgagcg gttttttgag taaaaatagt ttatgattat tcttaattaa acatctattt
     2461 atttttaaag ctttcattta tcgataagaa tagtttaact gttgatttat tccattttcc
     2521 gttcatctgg tacgatgcct tgacttagca accacttgtg gaagaaccat tctgccacac
     2581 cgatcaatag agcaggaatc aatactttca tccaaatatc gactgtatca ttcataaaat
     2641 atagatagag ccacaagata actgcagctg aaccagcatc tgcgatcgtc gctgcgatgt
     2701 tccctacttt acgtaagatc aacagatccc ctagcacata gatcatgact acaataatta
     2761 ccccaacaat cgttgaatcc ataaagctaa caccgtataa acctgtcagc acgacccaca
     2821 gaatcgccca gatcatgatt gccttcacta ccaacgcctt cacatgtttc attttctgca
     2881 ctcctttctc ctggataaaa aagtaagatt atctaacaag ataacctttt cattattata
     2941 ccatcggctg agagaagcga gcaaagataa ggtcctatct aaaaatgagg aagccactta
     3001 gaacattcac gtaataattt atccgtacag acattcgata tggtgttcct ttactttcgc
     3061 atactgttct tttgtcagtt ctttacttgt caccagataa tcgatttcat gaaaagcaat
     3121 ggcttgatgc atgaatttat gtgcaatttt ttgctgtgta actaaaacgg caagccggtc
     3181 tgattgtttt ctaaaagctt ttttgaccat tgcctctcct tcatgagttt ctgttactcc
     3241 ttggctgaaa tctagcccgc gacaactcat tatcgctaaa tccgcataat atgacaatgc
     3301 atcgttatag gcttttgaac cgttgattgt tgctcgtttc ggaacaattg cccctcccaa
     3361 tacagatact tttgccgttg tatattcact caacatagca gctgtgacga tgccattcgt
     3421 aatgatctgc aaaccatcaa atcttgctag gtttctgact aagtgtaaag aggttgtact
     3481 ggaatccaag aataagacca tatcatctcg aatcaaagaa gaagctgtat ctgcaatctt
     3541 acgtttttct gtttcttctt ctaatttggt gatcttcatc aggtaaggca cgtcaatttt
     3601 tcgattgtca ataagcatgg cgccaccgcg aactctacgg atcaatccac ttttttccag
     3661 aaaaacaagg tcacgtttaa tcgtggcttc gctataattt actaactcga tcaaatcggt
     3721 gactcttgca aattggttgt tttttagata attcacaatc tcctgctgtc tattctccac
     3781 ttcattcaac ataaaatcac cccatttact ttatatagta acaggaaaaa agaggttgtg
     3841 acagaagtga ttggcttcga gaaataagaa gaaatttttc gaaaaaacta cttctgaaca
     3901 tcgtgtgtgg ttaagagggt atgacaagac tattgtcata ccctcttgac cacacatttt
     3961 tatttttagt ataaactagc cttgcctact gtgttcaaaa tttccatttt atgtttgacc
     4021 acttcttgta cctttttatt agctgctgga taaattgcat tcggctcgta catcgatgga
     4081 ttttcactca atatttctct caaagcattg aaaaagacgc ttttaagatc tgagctcaaa
     4141 ttcactttgg caactccgta ttttactgct tcgctgactt ctgcgtccgg attacctgaa
     4201 ccgccatgta aaacaaatgg aagatcgatt tgttggttca attctttcag taagtcgata
     4261 tttaattttg gagttctgtt tttcggatac aagccatgcg ctgttccaat tgctacagca
     4321 agtgtatcga ttcctgtttc tttcacaaaa atcttcgctt gatcaggatc tgtataaatg
     4381 attgtttcag cacctccttc agcagaacca ttattcccaa tcgttccaag ctcagcctcc
     4441 actgagacat ttaccgcatg agctaacgca accacttttt tcgtaatctg tacattgtcg
     4501 tcgaatgatt tcaaagacgc atcgatcatc acggaagaat agccgtttcg aatcgcccgt
     4561 aagacatctt ctatcgtacc gccatgatcc aagtggataa cgatcggcag tttacttgta
     4621 taagcatagg ctcttactgc cgcaacaaac tcatcactta aataggcgat ttcatcagga
     4681 tgaatttcta agattactgg tgcttctaat ctctctgctt cttcgatgat tgctttcatc
     4741 atatcatagc tgcaaatatt gaaggctggt actgcaaatt gattctcttt agccactttc
     4801 aataactctt tcattgtata taacattacg cttccacctc atttgttaga ttttcatttt
     4861 tcgtccactt gtcttgtttt ttctctttca ctgttttttt caatagtcca agcataaagg
     4921 ctgtagtgac tgtacctgat gcaattgcca agacatacat aggtaaattg ttcataacat
     4981 ttggaattag catcacccaa atccctccat gaggtgccat tgaagtaact cctactgcca
     5041 tggaaatccc tgcagcgact gctgaacctg tcatcaaact aggtatcacg cggatcgggt
     5101 cagataccgc aaacggtata gcaccttctg taatatacga agcgcctaaa atccagcatg
     5161 attgacctga agtccgttct tctttggtaa atttatgttt gaataataaa gtggctaaag
     5221 caatacctag gggaggtgtc attcctgcag ccatacaagc agcaatcggt gcatttactc
     5281 cagctgacat caaaccgatc gagaaagtcg aagtcgattt attgatcggc ccacccatat
     5341 cagctgccat catgatcccg ataagtaatc cgaacaatag cccggatgta tttcccaagc
     5401 tattcagcca atttgtcaaa gcatccaaca cccatgtgat cggttgttca atcacaaata
     5461 ccatcaaaag cccaataatt aagatcgata aaaaggggac gacaatcagc tgataaatag
     5521 cagaaaattc tttttttacg ctaattttat ttgccagaaa atcacagata taccctgcta
     5581 gcacgccacc aatcattccg ccaagaaaac ctgaaccacc tgtagaggcc agtaatccgg
     5641 caacaattcc tggggcaaaa cctgcttttc cagcaatcga tgtagcaatc cccgcgccta
     5701 acgcaggaac catcatggca aacgctgtat ctcctccgat ccttgaaaat gcagctgcta
     5761 acacattata gtctgctgag ttgggatcag ccgctgtaat cccaaaggca aaactcagtg
     5821 cgatgatgat tccgcctgcg atcacaaatg gaagcatgta attcacacca ttcatgaaat
     5881 gattatagat tgtcggcttt ttctcatttg cagctggttc tttttcatta gacacaacga
     5941 atacgccaat atctctcaat gcttcgtcta ttactacttg cggttctttg attgctcggc
     6001 ttgtagagac acgctttact tttttcccgg caaatcggtc caattcgatt tctttatcta
     6061 ctgcaagaat gattgcatct gcctctttga tttcttgttc agataactga ttttctgttt
     6121 ttacaccttg tgtttcgaat tttgcttgat ggtttagatt tcttgctgtt acttctaatt
     6181 tttctgccga catataagta tgagcaattc cagtcgggca agcagttaca ccaacgattt
     6241 tcatgattat tttcctcctt tttcttcact atcgatactc ggacaaagac tctactattt
     6301 tcattcttga ctaatctgtc tgatgatatc tgctggcgta gtggcttctt ttattccctt
     6361 tctaaaatct gcattcatca atcgtctgga gagtgtgctg agaatctcta gatgcatatc
     6421 tgctgatttt tcaggaacta cgatggcaaa aatatattca attggcattt catctatccc
     6481 gctatagtcc gttaatttgg ctgtatgaaa aaaacacagt gctggttttg tcacatattt
     6541 gctttttgca tggggaatcc caaatccatc ttctattcct gtggagatct cctcctctcg
     6601 tttgaatagt gccttaacga ctttttgaac atttcttacc ttatttgttt ttttcaacac
     6661 atcccctatt cgctcaaata atgatccttt tgaatctatc gatggatcga aaacaatcag
     6721 ttcttctgcg atcaattgat caaacctcat aaaaaacctc tcccccacaa aaaatgttaa
     6781 cgttttcttg gaactgaggt cagtatagca tcgctagctg taccgttcaa tattatgaat
     6841 gaacagagga aaaagaccca tcatccgcgt gtataagtca ttaaaatgaa cgatctagct
     6901 cacaaacaat cgcaagtcta taacaaaaca aaaagccttc cttgaaagga aagcttagct
     6961 tctaatttta ggtaaatggt tttttacttc gaaataaccc ctcttcattt cgttaatata
     7021 aggtgttttt atcaagtgga taattaaaac tatatactag tttttcattg ttttgtgcat
     7081 tatgatccta cgactttttc agcatcaaac gaagttttaa attggcaaaa aacataaaaa
     7141 aacagactat ccctatcaga tagtctgcta aacgttgagt tatcagcgtt tttattattc
     7201 cgcagcgttt gcgtctttga gaccgtattt tttgttgaaa cggtccacac gtccgtctgc
     7261 ttgtgtgaat ttttgacgtc cagtgtagaa tggatgtgag tcagaagtta cttcgacacg
     7321 gatgactggg tatgtattac cgtcttccca ttcaactgtt tcttgtgaag tttttgttga
     7381 acctgacaag aatttgaaac cagttgttga atccatgaat actactgggt ggtaatctgg
     7441 atggatgttt tctttcatga ttttttcgct cctttgccct gctccatttg ctgaaccaga
     7501 gttgatttgt cgatttacaa cattaacatc ttaacatgtt tcagtcaagg ttgcaattat
     7561 cttttcagat ttctggtcgg atttttttta ccaaaggaga catcttgaaa ttctttgaaa
     7621 aattgctgat tgttttttgt tttacgcaaa aagcggatca gttgttccgt atattctaat
     7681 gaatctccag tcatattttt tcgaagtttc caaatttctt ctaattcttc acttggcatc
     7741 aataattctt cttttcgggt acctgatttt ttgatatcga tggctgggaa gatccgacgt
     7801 tcagataatt cacgtgaaag ttgtaattct agatttccag ttcctttgaa ttcttcatag
     7861 atcacgtcat ccatacggct tcctgtatcg actaaagcgg tcgctaaaat cgtcaaactg
     7921 ccaccttctt cgatgtttct tgctgccccg aaaaatttct taggtttata caatgcggct
     7981 gggtcaatcc cgccactcaa tgtacgtccg cttggtggaa cgactaagtt ataggcccga
     8041 gcaagtctag taatactatc catcaagata acgacatctc gtttatcttc gactaaacgc
     8101 attgcgcgtt ccaatactaa ttcagaaact cgagtatggt tttgcggctg cagatcaaat
     8161 gtagaagata ccacgtcgcc tttcacgctt cgttctagat ccgttacttc ttccggtcgt
     8221 tcatcaatca gtagaacgat taattctact tccggatagt tttcggtgat accatttgcg
     8281 atttctttta atacagaagt tttccctgct tttggcggtg cgacgatcaa tccacgctga
     8341 ccaaaaccga ttggagaaaa gatatcgatc attcgggtag atagtctttg cggtgttgtt
     8401 tccagttgaa gctgtctttg cggataaaga ggtgttaagg ctggaaaatg cggacgttct
     8461 tttgcttctt ccggatcttt tccattaacg ctttccacgt gcatcaaacc ataataacgt
     8521 tccgattctt taggtggacg ggcttttcct gccactttgt ccccatttct caaactgaat
     8581 cgtctgattt gtgaggcaga aatatagata tcttctgcac ttggtccata attgattggc
     8641 cgtaaaaaac cgtaaccatc ttgaccaacg atatccaata ttccttccat ataataaaat
     8701 ccttgtttct ctgcttgtgc acgaatcaca gctagagata attccttttt attcatctta
     8761 ctgtaataag gaattttaaa ttctttggca taactataaa cttcttttaa cgttttgttc
     8821 tctagttctg ccattgtcaa ataatcgctc attcagctcc cacttcctct atcatttcaa
     8881 ttgatgcccc taaggctgtt agtttctcga taatatgatc ataaccgcgt aagatataat
     8941 ctacattgta aatcgtcgtt gtcccttctg ccaatagtcc agcaatcact aaacaagcac
     9001 ctgcccgaag atcagaagcc acgacttctg tgccgtgaag tttatttgga ccattcacga
     9061 tgatcatatt tccttccacg gtaatatcag cgcccattcg tgctagttcc ggcacatggt
     9121 tcacacgttt cgcatagatc gtatcgatga tttctgaagt gcctgtagtc atcaatagta
     9181 acgcagtaag tggctgctgc agatctgtgg caaatcctgg ataaggatag gtcatgatgt
     9241 ttgcgggttt taattctttc gaggggtaaa cttcgatttc atcttcgctg attttcatat
     9301 ggacaccaat ttcctgcaat tttgctataa agctttccaa atgttcaaag atcacatttt
     9361 tcactttgat accatttcct actgcagctg ctaatgctag ataggttcct gcttcgatgc
     9421 ggtcaggaat catgaaatgc cgacagccat gtaatgtttc gactccttcg atacggatca
     9481 catctgtacc cgctcctcga acttttgccc ccatattatt cagtaaagtt gccacatcta
     9541 tgatttccgg ttctcgtgca gcgttttcaa ttactgtctg tccttttgct tttacagcag
     9601 ctaacatcac attgatcgtt gctccgactg aaaccacgtc catgaaaatc cgattgccac
     9661 gcaatccttt attttctgtc cgtaaataca tggcaccatg ttcatttgtc acttcagcac
     9721 ctagagcttc aaatcctttg acgtgtaggt caatcggtct agggcctaaa taacagccac
     9781 ctggcaggcc tactaccgct tcgccaaatt taccaagcag tgttcccata aaataataag
     9841 aagcacgcaa actattgatt tttccgtgcg gcatcggaat cgatacgact tctcgaggat
     9901 cgatttctag tatgttgttg gaaaaatgga cagttgcgcc catgatctct aaaatttcaa
     9961 ttaatgaatg aacatcttgt atgtccggca caccttccaa gatcactggg gaatcagcta
    10021 agattgcagc tggaatcaat gcgactgcac tattttttgc accgctgatc gttacttcac
    10081 cagctaaagg tcggtttcct ttgattacga tttttttcat ctgtttccac cttgtttatt
    10141 aatctttttt catggatatg cttgtatttt atcatagata aaaaaaagaa acgagcatgg
    10201 tctgccgttt taagtaaaaa agaatggaga gcgacttcaa aagtcgttct ccattcctat
    10261 atatcgtatc tgttatgtgg atgcttacgc tttgttagct gaaccgaacc agtcgatatg
    10321 agatttagca tcttcaacga ttgctgcttt acctggagct aataatttac gagggtcgaa
    10381 tcctttacct tctttatctt taccagcttc gatatattta cgagtagctg cagcaaatga
    10441 taattggaat tcagtgttaa catttacttt tgagataccc attgagatag ctttttcgat
    10501 ttgatcttga ggaatacctg atccgccatg taatactaaa ggcatgtctg gacctacagc
    10561 atcagcaatt gcttgtaagt ggtcaaaagc taatcctttc cagttttctg gatattgacc
    10621 atggatgtta ccgataccgc aagctaagta gtcgatacct gtagcaacca tttgtttgca
    10681 ttcttcaata tcagctaatt cgccgttacc gatgattccg tcttcttctc cgccgattga
    10741 accaacttca cattcaactg agataccttt tgcatgtgct ttttcaacaa cgtctttagc
    10801 taatttcaag ttttcttcaa atggtagatg tgaaccatcg aacattactg aagtatagcc
    10861 taattcgata cattccaaag cagcttcgta atcaccatgg tctaagtgga tagcaactgg
    10921 aacagtgatg ttcattgctt caactaaatc agtgattaaa tctttacaga ttttgtaacc
    10981 acccatgtat ttagctgcac ccattgatgt ttggataagt actggtgctt gttttgcttc
    11041 agccgcttca aggattgctt gagtccattc caagttgttt gtgttgaatc cgccgacagc
    11101 gtaaccgcct ttacgagcag cttttaaaaa ttcagctcct gatactactg gcataataaa
    11161 attcctccta agttttgtaa aactaaattt gttaagacaa ttcttaacc
//
LOCUS       DACEMO010000086        11162 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEMO010000086 DACEMO010000000
VERSION     DACEMO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.088"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..634
                     /locus_tag="IW728_001659"
     CDS             200..634
                     /locus_tag="IW728_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1025020.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            735..1826
                     /locus_tag="IW728_001660"
     CDS             735..1826
                     /locus_tag="IW728_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1025021.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            1856..2533
                     /locus_tag="IW728_001661"
     CDS             1856..2533
                     /locus_tag="IW728_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1025022.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            2865..4160
                     /locus_tag="IW728_001662"
     CDS             2865..4160
                     /locus_tag="IW728_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1025023.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            4150..5609
                     /locus_tag="IW728_001663"
                     /pseudo
     CDS             4150..5609
                     /locus_tag="IW728_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5745..7241
                     /gene="glpK"
                     /locus_tag="IW728_001664"
     CDS             5745..7241
                     /gene="glpK"
                     /locus_tag="IW728_001664"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1025024.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            7255..9078
                     /gene="glpO"
                     /locus_tag="IW728_001665"
     CDS             7255..9078
                     /gene="glpO"
                     /locus_tag="IW728_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1025025.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            9079..9795
                     /locus_tag="IW728_001666"
     CDS             9079..9795
                     /locus_tag="IW728_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1025026.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            10101..>11162
                     /locus_tag="IW728_001667"
     CDS             10101..>11162
                     /locus_tag="IW728_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1025027.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGM"
BASE COUNT         3764 a         1857 c         2518 g         3023 t
ORIGIN      
        1 atactcaaaa ctaaacgact ttttcacaac ctctgattca cggtgttcca gaaggagttt
       61 tgtcgaaaac aagccgaaat tctttgaaga tggctacttc tttttttcaa atctagacag
      121 aggaggcgga cattattccg tgttttttga caaaaaataa aaatcatact atgatatgta
      181 cgccaatgga ggggatgaaa tggaaaagcc aattgaaaaa gcgttagatg aatgtctttt
      241 ctttagcgtg aaaaagctag atcgcatgtt gaataaatta gctgatgaag cgtttagaag
      301 gacaggtctg gcaccaactt atggttttat tttattgata ttaaaagaaa aagatggaat
      361 accacaaaaa gatattgcac aaatgctcta ttccgcccca tcaacgattg ctcgttttgt
      421 tgaaaaatta gaatataaag gatacgtcaa aacagttagc gaaggtcgct tgtcattagt
      481 ttatcttaca gaacaaggac gccaatttgc taaggaagtc gatggtagct gggaagaact
      541 tcatcagtca tacaaagcag ttttgggaaa tgaagcaagt aaagaattag cccagacaat
      601 gaatgatgca acagataagt tacaaaaaaa ataaaatttt ttaagcaaat tagttgcatg
      661 tacaactaat cgagtggttt aatgatagat gattctcgat aggttaatca gagcgaatta
      721 tgggaggaat gacaatgtca caatttgatc aatcaattac tttaccttca ggagttcaat
      781 tgaagaaccg attgatgctg tcgcctatga cgacacaact cagctttttc aatggagtga
      841 tcaccgaaga tgagatcact tattataaac aacgttctaa aggtcttggt gctgttatta
      901 ctggtgctgc aaatgtccaa gatatcggaa aaggctggcc aggtgaactc agcatcgctc
      961 atgatgaaat gattcctcga ttaagtgagc tagcgaaagc aatccaagga gaaggcgcaa
     1021 aagcgattat tcagattttc cacggtggac gaatgacaag tcgagcaact ttatctggag
     1081 aacagcctgt atctgcaagt gcagttgcag caaaacgtcc tgatgcggaa acaccacgag
     1141 caatgagtga agaagaaatc gttgaaacaa tccaagcatt tggcaaagcc acacgtagag
     1201 cgattgaatc aggatttgat ggaatcgagt tgcatggagc aaatacgtat ttgatccaac
     1261 aatttttctc tccgcattcg aatagacgag aggatcaatg gggcggtagc ttggaaaatc
     1321 gttatcgttt tattgaagaa ttattaacaa cggttttctc agcggttgaa caatatgcta
     1381 aaaaaccatt catcgtaggg tatcgtttct caccagaaga atatgaaaca ccaggtatcc
     1441 gttttgaaga cacgatctgg cttcttgaac gattacgtga gtcaaaacta gattatctgc
     1501 atgtttcatt aaatatgtat gatcgaatag ctcgttctga taaatacaac gataaatcga
     1561 ttcttgaagt ggtccataac acgattcaag gaaagatccc tttagttggt gtcggtggga
     1621 tccgcaatcg cgaagatgta gagcaggtat tgaaatatgc agaacttgca gcaattggac
     1681 agcaaatgat cgtcgatcct gactgggcag agaaattatt ggaaaaccgt gaggatgaat
     1741 ttgtaacgaa accttttgaa aaagcatata aagagcttta tttgccaagc ccattatata
     1801 atttcttgaa agcacgttat caataacgca atttttatag aaagaggatg ggaaaatgaa
     1861 agtattgatc gtattaacaa atgtagaaaa gtacgaaaag atcgagcgtc ctacaggttt
     1921 gtggttaagt gaactgactc atttttatga tgtattagta aaaaatggga tcgatgcaga
     1981 ttttgtcagt cctaaaggcg gatatgtacc tttagaaccg caaagtgttt tgaatatgga
     2041 tgaaatcgat tgggcctatt atgaagatga agatttcaga aatcgggcct tgggaaatac
     2101 cttacgtcct gatgatgtgg atgcaaatga ttatgcagcg gtttattatg ctggtggcca
     2161 cggaacaatg tgggattttc cagaagcaat agatcttggg aaagtcgctt cacaaatcta
     2221 tcgaaatgaa gggctggtct cagctgtttg tcacggtgtt gtaggattgt tgccagttac
     2281 agatgagtct ggaaaatcta ttttagctgg acgtacagtg actgggttca gtaatgaaga
     2341 agaggaagca aatggtacaa cgaatgaagt accattctta gcagaagatg ctttgaaaga
     2401 aaaaggagca aactatcagt ctggagcagc tttttcagaa gtggttcaaa ttgatgggcg
     2461 gcttcttaca gggcaaaatc ctcaatctgc tcatcaatta ggcgaagcag ttgttgaagc
     2521 attaaagaat taaaaataaa tgatgtgtct attctaacta ttacattaca cacgctaaaa
     2581 ctcgtctttt cgttcttttc tgtttcctat ttggacagag gtacgtaaaa ggcgagtttt
     2641 tttcttttat aagagtacat tttttgaaac atctcttcag aaatgatact cttcaattag
     2701 gtccactagt acattattga gaagcagacc tagaacacgg tatgatggag agggttgatt
     2761 ctgctgcatc gctatgtatc ataaggttga aagcgagtta tgaatactgt gttttctgat
     2821 tgagaagtta gcagtagaac accttataag aaagaggaat gcagatgaac attgtaattg
     2881 tcggcgggtc gtttgcaggg attcatgcgg ctatttatct tcgccaattg atgccaacaa
     2941 gtgaaatata tttgattgaa aagcaatcga agttaggatg gattcccagc gggttcaatt
     3001 tgatattaaa aggaaaggtt accagtgaaa aacaactttc ttggatcacg aaagaagaac
     3061 tgactgaccg ctatcgaatc catgtttaca cagaaaagac agtaatagga ttgaaagaaa
     3121 aaaaagtcgt tttagcagga ggaaaaaaat tggattttga tcgtttgatt ctagcgactg
     3181 gttcaaatca aaagtttcga aatatttctg cagaatcaac gcttattcac ccagttaaaa
     3241 agatacaaaa cgtaacggct cttcagcaaa agatcatgaa tgcaaagaaa attgcaatta
     3301 ttggtgcagg acaagcagga atcgaaatag ctgaagggtt ggcatcaaga aaaaaacaaa
     3361 tccatcttta cgaaagccga aagtcaattc tttttcgtta tctagatcct gaaatgacag
     3421 aaccgttagt aaaggaaatg aaaaatcaag ggctttcact ttttttagaa gaacaagttg
     3481 tgtctttgac cgaaaatgaa accgctattg tagagacgga aaaaagaaag gagaactatg
     3541 atcttgtttt gctggctaat cattctcgtc ccgacaatca gatgtgggaa gaacaattaa
     3601 cattgaatga tgatggaacg atctgggtag atgactactt gcaaacgtct caaaaagacg
     3661 tttatgcgat tggcgatgcg atccaagtaa cttttcgtcc aacaaatgaa aaaatgtatg
     3721 tttctctagt aaataacgct gtccgtacag cacgaaatgt cagcaagaca attagtggct
     3781 cacagacaaa ggatcaggga acctatcgtc cagtcggcaa tcagtggttt ggctatttct
     3841 taggaagcgt aggtttgaca gaagaagaga gcatttttta tccccaaaaa attgaaacag
     3901 gatacgtaga ctacaagctt acagcaataa atcaaagcaa agttaaactc aaatttctat
     3961 ttgattctga aaaaaagatt cttggagcac agctccaaag caaaaaagag atgttccacc
     4021 ttcttgacca actgacaatt gcagtagaag aaggatggac gtttgaacag ttagaagaac
     4081 atgagctatt tttccagcca gaataccgtg tgccagatat ggtgtggtca aaggtggcga
     4141 atactgccta tgaagattga agcattatta gaaagaaaag agcagttaca gatcctgatt
     4201 ttgcgaaacc ttgttctgca agggggaaca gcctctatca atggtttgag agagcatgtc
     4261 caactgtcaa aagcctcgtt tgatcaatat ttggaagata ttgaattgat tggccgaatg
     4321 atggaaaaaa agtagaagta cagcgaaatg agtatcaggc gctgcttgtg ttggacgaag
     4381 atgtaagttt ggaaaaaatt ctattgtttt tattgcagga aagcttgaag tttaagatgc
     4441 tcgtctattt attagaacat cagcaagtat ccatcgttcg attagcgaca gcgtttaata
     4501 tcagtgagtc ttctgttttt cgaaagatca aagaattgaa ccagctctta gaggaatttg
     4561 gattacaaat caaaaacggt caattatacg gagaagagct acagatacga tatttttatt
     4621 atgaactatt ccaatatatc ccggaagatc agcgaccgct ttttttgcaa aatacaccag
     4681 aaaaaagacc gttcattcta ggattggacc gtgtattaga aacaacgttt acggcatcag
     4741 cagaagcaca gattgcttgc tggctcggga ttacgaaaaa aagactattg aatgaaaaaa
     4801 gtacgtacgc aactttgaaa gaaaagaaat tactctatca gtcggaccgg ctttatcaag
     4861 caatcgatcc aatcatcgtg atgcatctta gtcgaacagc agcagaactg aatgtatacg
     4921 aaacaatgat gttttacagc ttttttgttt ctttttcgat agtagacgaa gaaatatttt
     4981 atcagtatga tttgacacgg agcaaaaagt taccaaccgc tgttttagat acttacattc
     5041 gggaaacgat gttatggcat tatcgtcctc gtcgtttgaa aatcaaagaa gaaaaggcag
     5101 taggctacca aatttcccag atcaacaatg aatggttttt ctttgttgga aagatcgaag
     5161 tctatgagcg tgatcggctt ttggaacagc agcagaaaat gctcgggcat tccttaacac
     5221 agttactggc gaaattacag gaaacagcgg tgcagcaatt gccgaggaaa agagctgaag
     5281 atagtgagct tagttacctg atgatccagt atgccaatgt tcttttgatg atcgattttt
     5341 atattgcaaa accagtagtg attggaatcg atttagaaag tctgccgatt tatcgtattg
     5401 cttttcaaca atatctgatc agagaactga ggggaatcgg cggtattgag attggaagtt
     5461 atgaagaagg taaagaatat gatttggtga tcacattttg ccagagaaac aaaaaacagt
     5521 gtgaatatta tctgtcagaa tttgcgtcac cttacgacat tattcgattg aaaagaagaa
     5581 tagagacgct gaaaaaagaa aagaattgat taaaaaatag cttcataata aaaaactgtc
     5641 aaaaaaagaa aaaaagaata aaattttgac agtttctttt tttaaaacag tagtatgata
     5701 agagtagtta aagaaagcgt ttgcatataa tggagggaat cacaatgaca gaatcaacgt
     5761 atatcatggc gattgaccaa ggtacaacta gctcacgagc aattatcttt gacaagaaag
     5821 gaagacacat cggcagttct caaaaagagt tcacgcaata tttcccgcaa gaaagttggg
     5881 tggaacatga tgcaaatgaa atctggaatt ctgtacagtc agttattgcc ggagcgttca
     5941 tcgaatcagg gatcaaacca aatcagattg ctgggattgg cataacgaac cagcgtgaaa
     6001 caacagtaat atgggaaaaa gatacaggac gtccaatcta tcatgcagtt gtttggcaat
     6061 ccagacagtc agctggaatt gcagataact taaaaaatga aggctatcaa gaatttttcc
     6121 acgaaaaaac agggttagtg atcgatgcct atttctcagc aacgaagata cgctggattc
     6181 ttgatcatgt agaaggcgcg caagaacgag ctgaaaaagg cgaattgatg tttggaacga
     6241 tcgattcttg gttagtatgg aaactgaccg atggacaagc acatgttact gattactcga
     6301 atgccagtcg gacgatgttg ttcaatatcc atcagctaga ttgggatcag gagattttag
     6361 atttactaaa tattcctcga gtgatgttac caaaagtcac ttctaactca gaagtttatg
     6421 gttatacgca aggttatcat ttttatggaa gcgaagcacc aatttcaggg atggcaggag
     6481 accagcaagc cgcattattc ggacaaatgg cattcgaacc aggcatggtc aagaatactt
     6541 atggtacagg ttcatttatt gtcatgaata ccggtgaaaa accgcaatta tccaaaaata
     6601 atttattgac gacaattggt tacggcataa atggaaaggt ttactatgct ttagaaggaa
     6661 gtatttttgt ggctggttct tcgattcaat ggctgagaga tggacttcaa atgttgcaaa
     6721 aagctagcga ttcagaagac gcagcgaaac gctctacaag cgaagatgag gtttatgttg
     6781 ttcctgcatt cgttgggcta ggtgctcctt attgggatca ggcagcacga ggggctatgt
     6841 ttggcttgac acgtggtacg acaaaagaag atatcattaa agctacttta cagtcgattg
     6901 cttatcaagt gagagatatc atcgatacga tgcaagatga cactggaatc aagattcctg
     6961 tattgaaagt agatggcggt gcagcaaaca atgaatatct gatgcaattc caaacagata
     7021 tcttgaatgt accgatccaa cgagcagaaa acttggaaac aacggcgcta ggggcagcat
     7081 ttttagcagg attagctgtc ggttattgga aagatacaga cgagatccgc gaattttatg
     7141 aagctggcaa gctatttgaa gttcaaatgg aggaagagcg ccgagaaaaa ctttacaacg
     7201 gttggaaaaa agcagtaaaa gctacccaag catttgaata aggaggaaat ccatatgttt
     7261 tcaaacaaga caagacaaga tagcattcaa aaaatgcagc aagaagaatt ggatctgttg
     7321 atcatcggtg gcggaatcac tggtgccggt gtggcagtcc agtcagcagc atcaggaatc
     7381 aaaacaggat tgatcgaaat gcaggatttt gcagaaggga cttcctctcg ctcgaccaaa
     7441 cttgttcatg gcggtattcg ttatctgaag acatttgatg tggaagtagt agctgacaca
     7501 gttggtgaac gtgcagtcgt acaaagtatt gccccacaca tcccaaaacc agatccaatg
     7561 cttttgccaa tctatgaggg cgaaggagca acaaccttca atatgttctc ggtcaaagta
     7621 gcaatggacc tttatgacaa actggcaaat gtgacaggaa ccaaatatga gaattatacg
     7681 ctgacaccag aagaagtatt ggaaagagaa ccgtttttga aaaaagaagg gctaaaaggc
     7741 gcgggtgtct atctagattt ccgcaacaat gatgcccgtt tagtgattga taatatcaaa
     7801 aaggctgccg aagatggggc ttatctagta agtaaaatga aagcggttgg ctttttatat
     7861 gatggcgatc aaatcgttgg cgtcaaagct cgtgacctgc tgacagatga agtgatcgaa
     7921 atcaaagcaa aattagtgat caatacgagt ggtccttggg tagataaagt acgaaacttg
     7981 aattttacgc gtccagtctc tcctaaaatg cgtccaacta aagggatcca tttagttgta
     8041 gatgcgaaaa aactgcctgt accgcaaccc acatacttcg atacaggaaa gcaagatggg
     8101 cggatggttt ttgctattcc aagagaaaac aagacttact ttggtacgac agatacggat
     8161 taccaaggag attttacgga tccgaaagtc acacaagaag atgtagatta tctattggat
     8221 gtgatcaatc atcgctatcc agaagcaaat atcacattga atgatatcga agcaagctgg
     8281 gcaggacttc gtccactgtt gattggtaat tctggttctg attacaatgg tggagataat
     8341 ggatcgattt cagacaagag cttcaataaa gttgtcgata cagtaagtga atataaggaa
     8401 aacaaagttt ctcgtgctga agtagaagac gtgttgaacc atttggaaaa cagccgtgat
     8461 gaaaaagcac cttctacgat ttccagaggt agttctttag aaagagaacc agatggtttg
     8521 ctgactttat ctggtgggaa aatcaccgat taccgtaaga tggcagaagg agctttgcga
     8581 ttgatacgcc aattattaaa agaagaatac ggaatagaga cgaaagaaat cgactctaaa
     8641 aaatatcaga tttcaggtgg aaacttcgat ccaactaaat tagaagaaac agtgacagaa
     8701 ttagcaaaag aaggagtagc agccggttta gaggaagaag atgctactta tatcgctgat
     8761 ttttacggga ctaatgcccg acgtatcttt gaattagcaa aagaaatggc accttatcct
     8821 ggtttgagtc tcgctgagtc agctcggtta cgttatggtt tagaagaaga aatggttttg
     8881 gccccaggcg attatctcat tcgtcgtaca aatcatctgt tgtttgaacg agatcagctt
     8941 gatgagatca agcaacctgt gatcgatgca attgctggat attttgaatg gacagaagag
     9001 gagaaggcgc aacagactaa acgtttagaa gcattgattg cagaatcaga tctgcgggaa
     9061 ctaaaggggg agaaataaat gaacagtgat atgactcaaa tcatggggga gtttattggg
     9121 acactgatcc tagttttgct tggagatggt gtgtgtgcgg ctgtgaattt aaacaaaagt
     9181 aaagcacagg cttctggctg gattgtcatc gcatttggtt ggggactcgc tgtaacaatg
     9241 gctgtctata tttctggatt catgggccca gctcatttga atcctgcagt ctctctagct
     9301 atggcgatga caggggcaat tagttggaat cttgtcgttc ctttcatcat tgcgcaagtt
     9361 ttaggagctt ttgcaggggc cattcttgtt tggctttctt atttgccaca ttggaatgca
     9421 acgaaagatg aaagcgcgat tttaggaacg tttgcaacag gtcctgctat acggaattat
     9481 ccagcaaatg tgatcacaga attaattggt acatttgtat tagttcttgg gttgctagct
     9541 tttggacaaa acgaatttgc acctggaacg aacgtattcg ctgtaggtgg attgatctta
     9601 gccatcggtc tttcattagg agggccaaca ggatatgcaa tcaaccctgc tcgggacttt
     9661 ggtcctcgat tagcacatgc ggtattgcca atagctaata aaggaacttc agattgggca
     9721 tattcatggg taccaatcgc aggtccaatg atcggagcaa ttatcgcggt aggtgtctat
     9781 tcattaatgg tgtaaatcta taaaacatac taaagtttga caaccaaaat gtgtcttgct
     9841 atagttaagt ggaaaataaa taagacctag aagaatgggg tatgttttgt gagtgtagct
     9901 ggtatctaat cagtaagtcg aatagccaat aaacaaagtt tatttggtgt gcttatgaaa
     9961 cggtacctgt caggaaaaca atatctttgt attctaataa ggataggaca tgactttttt
    10021 aagctgtgtt ctatcctttt atttatctct aggcccttat ttattttggg acaggtatct
    10081 cttcaagaaa aggagagaat atggacaacg aaatgattag tcaattacaa tttgatcgga
    10141 tcaaaaagga aatccaagca agagcaattg gaaactatag caaaaaacga atcagcgata
    10201 tgacggtctc gacaaaccta caaactgtct tggatcgtca agaagaaaca agagaagcac
    10261 gcttgatctt agaaagcagt cagcatgttc catttatggg gttgccgagg atcgatgcac
    10321 tgacagaaca agtcaaaaaa gggttgattc tgcagccaac cgatttgatc gaatatgcgg
    10381 attttcttag aagcagtcgg atgatcacaa agttttttga taagaatcaa taccagacac
    10441 ccttgttgtt tgcttatagc aaacatcttc ctgatttgat aaatgtagaa gagttgattg
    10501 accagaaaat aaaaaataat aaagtcagtg acgacgcgtc acgtaatttg cgtaaagtac
    10561 gcaaacaatt acaactaatc gaaaaagaaa tccagtcaaa attactgaaa ttcttgcgtc
    10621 atccgaaaaa caaagaaatg atccaagaag caatgattgt tcaaaaaggc gaatactata
    10681 cgattcctat caaagccagc tataaaaata aaatagatgg aacgattatc gatgaatcaa
    10741 ataaaagaac gacggttttt attgaaccaa ctgtgatttc taaattgaat gaacactatc
    10801 agttgttaaa agctgaagaa atcagtgaag aatatcaagt cctggcggca ttgactggtg
    10861 ctatcgcaga aaacgaagaa gtgatcgatt tattgatcga aacgatcacg gtgttagata
    10921 tcatctttgc tcgggcaaaa tttagccgag aaatcaatgg aagcacacct agaatcaata
    10981 aatcggaaca tatcattatc aagcaaggca gacatccatt tttacctgag catgcggtac
    11041 cattggatgt tgaaatcggt aaagactatc gaggcttgat tatcaccgga gcaaatgccg
    11101 gaggcaaaac agtcgtgttg aagacagtcg gtttattgac tttgatggcg atgttcggta
    11161 tg
//
LOCUS       DACEMO010000087        11156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEMO010000087 DACEMO010000000
VERSION     DACEMO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.089"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            214..1500
                     /gene="efmA"
                     /locus_tag="IW728_001668"
     CDS             214..1500
                     /gene="efmA"
                     /locus_tag="IW728_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1025028.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1638..2237)
                     /locus_tag="IW728_001669"
     CDS             complement(1638..2237)
                     /locus_tag="IW728_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1025029.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2641..3762
                     /gene="mnmA"
                     /locus_tag="IW728_001670"
     CDS             2641..3762
                     /gene="mnmA"
                     /locus_tag="IW728_001670"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1025030.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            4008..5429
                     /gene="arcD"
                     /locus_tag="IW728_001671"
     CDS             4008..5429
                     /gene="arcD"
                     /locus_tag="IW728_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1025031.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5443..6561
                     /locus_tag="IW728_001672"
     CDS             5443..6561
                     /locus_tag="IW728_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1025032.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6621..7913)
                     /locus_tag="IW728_001673"
     CDS             complement(6621..7913)
                     /locus_tag="IW728_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1025033.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            8063..8623
                     /locus_tag="IW728_001674"
     CDS             8063..8623
                     /locus_tag="IW728_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1025034.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8752..9129
                     /locus_tag="IW728_001675"
     CDS             8752..9129
                     /locus_tag="IW728_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1025035.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            9062..11134
                     /locus_tag="IW728_001676"
     CDS             9062..11134
                     /locus_tag="IW728_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1025036.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         3593 a         1993 c         2410 g         3160 t
ORIGIN      
        1 ttgtaaggac gacgcaaatt agttgtcttt tttattttta aatttaacac atgttgttat
       61 ttttaatatt ttgctgattt attgtttcta agtaaagaaa tttaaattga aacgattgtt
      121 atttagggaa aaaggtattt tttgaaaaaa gaatcccgta taatgattgt tgtgcaaacg
      181 ctatacaaaa tgaaaaagaa aagagggatg tttatggaaa atgaacagtc ggtcgtttta
      241 acgaattgga agagaaatta tttgttcttt ttatcgggac agtttttgtc aggaatcact
      301 agtatggtcg tccaatatgc gatcatctgg tatttgacaa gagaaaccgg ttcggcgaca
      361 attttgagtt ttgctacttt actaggaatg attcccatgg ttttactaag cccatttgta
      421 ggaccgctag tggatcgctg ggataaaaaa gctcttttga ttgtcacgga tattattgta
      481 gcgattttcg cgcttatttt agcagtagtc ggaacgattt cagaatcctt cccgatttgg
      541 ctagtttttg tgtctttgtt tatgcgttcg gtcgcacaaa cattccaaat gccaacgatc
      601 cagtcgatta tgccgacaat cgttccatct tctcacataa caaggacgaa tggacagtta
      661 ggaatggtcc aatcagcgaa tttcatcatt gcgccagctc taggtgcggc tttgttttca
      721 gtcgttcctg tcaattacct tattttatta gatgtattgg gggcagtttt tggtgtcggc
      781 ttattgattt ttgtgaagat cccaaaagtt tctcctgaaa tattggaagt tccgcttact
      841 atttttaaag atgcaaaatt tgggctgcag caattgatgg ataataaagg gttatggtac
      901 ataacgatca atggtgcatt tgtgatgctg ttgttcatgc cagcaatcag tctgtatcca
      961 ttgatgacgc tagattactt tggtggttcc gttggacagg cgggagcagt ggaagtagtc
     1021 tacgcagtcg gaatgctctt aggcggtgca ctgatcagtt ttataggaac atggaaagac
     1081 cgaatgaaac ctattattat agcctacatc attatgggcc tgacgatcgg tgcaagcgga
     1141 ttggttccaa acgatagtca agggttcttg tactttctta tcttgaatgc aggagcaggt
     1201 tgtgcaacgc catatttcaa tacactgctg atggcaatga tccagcagag ttatgaatcg
     1261 aatgttttag gtcgtgtctt agggaacttc aactctttga tgaacctggc aggtccaatt
     1321 ggattattat ttgcaggacc attagctgat cgtttagggg tagaaaaaat gttcttgttt
     1381 tccggaatcg ggattttatt atgcggaatc gttttatttt taacgtcagc tgcccgaaat
     1441 tatgataaag aattacagaa aaaactggta aaagaacacc atgagcaaaa agacgaatag
     1501 tatggaatgg agttgtgaca gaagtcttgt tacaactcca tttctgatcc taatgttcct
     1561 gtggcaataa gcctactcaa atgaaaaata tgagtaatta tctttatttg tttaatactt
     1621 gatatagggc agttttttca cgacctatcg tacttcctct ttgcttcata gatttctttg
     1681 atttgttgct cagataacgg agtgccaaat ggtagttttt cctttaagag ttctctttcc
     1741 ggtgcgtcta cgcctacatt gacatcgttc tcgataggta cgctgtaatg atggatatgt
     1801 ccatgaagat tgcggatagt gtcgttaggc ccgagcaaca tcggataatg agtcaagtaa
     1861 tattgttgat ggtcgaattt aacgatggca cctacatcat aaaaataaaa tttgggactt
     1921 ccgtcagtaa gcctaggatc atttttttct aaataacgga aaaaagcgcg actgtcatgg
     1981 ttccctttga tcaagtggat tgttccttgc agttgagtga ccagttccaa aatttctgca
     2041 tttgcttttt catattgcgg atgcatagca aggtcgccta gatgataaac atggtcggtt
     2101 tcttttatta ctgcattcca gctagtgatc aactgttggt tcatttcatc cacatttgca
     2161 aagagccgcg gagcaaagtc gttttttcct aataagcgtt catggaaaaa gtgcatatca
     2221 ctgataaagt aattcatgta tcttttgcct cccttttttc ttagagtaaa caaaatcact
     2281 tatctgagca actcataaca tctaaaaaga aaaggttgta aatgagacgt gcagctttaa
     2341 gcgataagaa gcaattttga aaaatagttt ttcacaattt ataaaattat tccttattgc
     2401 cgaaaggctg gcccttttta tgagtatgcc aattttttat atcgaatgct caaaagatga
     2461 gtgatttttt ggttgactat catgtttgtg tactgaatag gtccagtaga acagtatttt
     2521 tacgacttga taattaaggg atgaaatttt cagaaatatg acgtataatg taaaatactg
     2581 aaaagttgcg accttcaact cgtagatgtt tcagaatcta attgaaatga tggtgatatc
     2641 atgacagaca acagcaaaac acgcgttgtc gtagggatga gcggcggtgt cgactcatct
     2701 gtaacagcac ttttgttaaa ggaacaagga tacgacgtga tcggaatatt catgaaaaat
     2761 tgggatgaca cggacgaaaa cggtgtatgc acggccacgg aagattataa ggatgtagcg
     2821 aaagtagctg ctcaaatcgg gattccttat tactctgtga attttgaaaa agaatattgg
     2881 gatcgtgtat ttgaatattt cctagccgaa taccgcgcag gacgtacacc taacccagac
     2941 gtgatgtgca acaaagaaat caaattcaaa gcattcttgg attatgcaat ggatcttggc
     3001 gccgattatg tagctactgg acattatgcg caagtcactc gcgatgaaaa cggcgtggtc
     3061 cacatgcttc gtggagtaga caataataaa gatcaaactt acttccttag ccaattatca
     3121 caagaacagc tttcaaaaac gatgttccct ctaggcggca tggaaaaatc agaagtacgg
     3181 gcaattgctg aacgcgcagg ccttgcgact gctaagaaaa aagattcaac gggaatctgc
     3241 tttattggtg aaaagaactt caaacaattt ttgagcaact atctgccagc aaaaaaagga
     3301 aatatggtca ctcttgatgg tgaagtcaaa ggacaacatg ctggcttgat gtattatacg
     3361 attggtcaac gtcaaggact agggatcggt ggcggaggag attcgcaaga accctggttt
     3421 gttgtaggaa aagatctagc gacaaacaca ttatatgtgg ggcaagggtt ccatcatcct
     3481 gcattgtatg ccacaagctt ggatgcaagt gaaatccact ttacgacgaa tgaaccaatg
     3541 ccaaaagaat tcaagtgtac agcgaaattc cgttatcgtc agcaagacgt tcctgtgaca
     3601 gttcgcttat tggatgataa tcgtgcagaa gtcgtattcg acgaacctgt acgtgcaatc
     3661 actcctggac aagcagttgt attttatgat ggaatggaat gtctgggtgg tggtttgatc
     3721 gaccacgcat atcaagaaac aaaagtttta caatacgttt aagcgatatt aaatgaatca
     3781 aaacagctat ggctaacaaa agaaacacct tttcttttgg atagcatagc tgtttttttg
     3841 caataaaatt aatttttatt cattttatac ttgaaatact tgtgtaaaaa gttcataata
     3901 tccaataata tgaataaagt tgctttttgt taagcaagag cagttgcaaa atagaaggga
     3961 atcttttgca agtgatttgt tcaattttat aagtgagggt gatggaaatg gaaaagaaat
     4021 cgacgggtat tagcaaatgg ggactggttg ctctggttgt cagttcgtca atcggaagcg
     4081 gtgtgtttgg gatcacaagt gatctggcta gttctgcagc tccagggcca gcgatccttt
     4141 catggataat cgttgggatt ggtattttag ctttggtatt atcgctgaat catttaggag
     4201 aaaagcgtcc agatctagat ggtggtattt tcggttacgc ggaagcttca tttggtaaat
     4261 taggtggctt catcagtggt tggggttact ggttgtctgc gtggctggga aatgttgctt
     4321 ttgcaacgat gctgatgagc gccatgggag aatttttccc gatattcaaa ggtggacaaa
     4381 atgttccttc tattttattt gcaagtattt ttatttgggg tctgacttta ttagtaaaca
     4441 atggagttga aggagcgagc gtcatcaata caatcgtgac gatctgtaaa ttagtgccat
     4501 tattcctatt tttaatattt gtatttatcg catttaaagt gaatatattc actgctgatt
     4561 tttggggtaa cgtatctgat aatctagtga atggaacagg gcagcaagga acgatctggt
     4621 tgcagattaa aggttgtttg atggttatga tgtgggtctt tgtagggatc gaaggcgcaa
     4681 gtgttttagc caaccgcgcg aagaaacgtt cagaagccca gcaagcatcg atcattggtc
     4741 tgctttgttt attgtttatc tatatccttg cttcgatctt gccatacggc gtcttatctc
     4801 aagaagagtt agcaactatt tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg
     4861 gaaattgggg agcagcgctg atcaatatcg gcttgattat ttcgatcatt ggttcttggt
     4921 tgtcttggac gatgctccca gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc
     4981 cgaaaggttg gggaaaaacg aataaaaaaa atgccccaac ttattcgtta gttgttactg
     5041 caatcttgac taatctattc ttattgactt tcttagttac tgattatgcc tatcaatttg
     5101 cctattcttt atgtacagcg gcaattttga tttgttatct acttgtcggg atctatcaag
     5161 tccagttttc tttaaaagaa ggaatcaaaa aacaagtagt gatcggtggg attgctgtag
     5221 tttttgaatt gatgggcatc attttagcag gattttctta tgtcctttta tgtagtattg
     5281 cctatcttcc tggtttttgc ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta
     5341 cgggaaaaga aaaagtaatg attgggctga tttgtgtggc cgcactgctt tcaatcgggt
     5401 tgcttgctgt aggctggatc aagatataag ggggaaagaa acatgaaaat cgcaaatttc
     5461 ggtgtagaag aatggctaaa taaatgggaa aagaaagcgg tatatgatat tgcacaaagc
     5521 tcgatcgaag cattgacatt agacgaactc gttggcttag atgggactag cgtaagtaaa
     5581 tttttcgaaa aacgcaaaaa cgaaccattg gactatggct ggatcgaagg atcagatgtt
     5641 ttcaaacaag aagtcgcttc tctttatcaa accgtcgatc cggaaaacat cttacaaacg
     5701 aatggagcga ctggtgctaa tcttttagct ctatacgcgt tagtggaagc aggagaccat
     5761 gttgtttcga tgcttcctac ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc
     5821 gctgttgact ttgttcattt gaaggaagaa gaagattggc agtttgattt agaacagcta
     5881 gaagccttag tcaaacctga aacaaaaatc atctgtttga atagtgcaaa caatcctaca
     5941 ggcacattgc tagatcggaa aactttagaa aaaatcgcag aaattgctcg tacagtagat
     6001 gcatatgtat tgatagatga agtatatgcg cctttaacgg ataaaggtga attcttgtcg
     6061 atcgtagatg tctatgaaaa aggtatagca accaattccc tatccaaaac ctactccatt
     6121 ccaggtattc gtattggctg gacagctact ggacctgagt tggcagaagt ttttcgaaag
     6181 tatcgagact acacgatgat ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca
     6241 ttgaagaata aagaaaaaat attggcgcga aacaaaaaga tcattacaga gaaccttgtg
     6301 attttaaaac aatgggtagc caatgaaccg aaagttgaac ttgtcgctcc caattacgtc
     6361 tctacttctt tcattaaatt agccatcgaa gaagatgacc agacattttg tatcaattta
     6421 ttagaagaaa caggagtact attagtacca ggaagtgcct ttgatttacc gaagcatgcc
     6481 cgactaggtt actgttgtaa aaaagaaacc ttggaaaagg gattattcct cttatctgag
     6541 ttcttgaaaa aacaggccta aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa
     6601 gcagtgtttt tcatttacgc ttattgtcta tgagaaggag agtgccctga agctatggag
     6661 actcctgaag ctttttcttc tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt
     6721 acaagcaaga agttgaccaa aacaagaaga gaagtcgaaa gaaagacgcc gcgataacca
     6781 aatgcactgg aaacactgga gccgatcata ggccctacta cgttaccagt tgcttggaaa
     6841 gattggttat aactgaagat ccgtccagca gcttgatgag gcgaatattt tgtgatcaat
     6901 gcttggacag cagggagcag acaagcatca gagataccaa tcaaaaaacg taaggcagct
     6961 aattgccaaa cattcgtgac gaatgccatt gggatataaa ccagaatcgc aaaacccaaa
     7021 ccaatcgcaa gtattctttc actcccaatc ttgtcaccta agcgaccaaa tcttggagcg
     7081 gctatcaaag tggcaatccc aggtatcgaa gcaatgattc cgctgatcaa cgtaacattc
     7141 ccatgtccat gcagcaattg gcgaatgtac agactgatga ttggactaat ggaattgttg
     7201 gatgcttgga tgatcatcgt tgtgacgaac ataccgatga cgacgtgtgg atatttcaag
     7261 tctttgaaaa tctgtttggc agaaaccatg ttttcttttt tcactgggac aaatttttca
     7321 tggacgaaaa acagactcaa caagaagacg aaaaataaga tgatccctgt aatgaaaaag
     7381 gtagggcgat agccaaacgc tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt
     7441 cctgtaacag aaccagttgc taaggttcca agaacttgac cacttttttc ttttggcgtc
     7501 ccagttgcga caagggcagt ggcattactg atataaccag aaaaaatccc ttgaagcaga
     7561 cgcaatgcga ctaattgata cacactagtc acgcaaccca tcaaagaaat gacgattgcc
     7621 attcctaatg aagcacgcaa cagcatcaat tttcttcctt tttggtcggc taattttccc
     7681 caccaaggag agataagcgt agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa
     7741 aagttcaatt gtgacgtact ataattacct aacgtatcta tgtaaagtga cataaaaggc
     7801 atgactaaac taaatccgat accagccata aatgttccaa accataagac aaacaagttt
     7861 tgttcccaag tttctctttt cctaaataat ttttccttta ttcttgcagc caattagact
     7921 ccttctttct gaaaattttc atctacagct ctttattata aactacaata tgtagtttat
     7981 caatgaaagt ctcttttttc tctttctttt tcgtggtaaa ctgaaagagt gaaggattta
     8041 ttttatgaga ggaaacatgg aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga
     8101 ctgctttcga gaactagcag aagagatgga tgttcagcaa ataacctttc agcatttagc
     8161 caaacgcttg gatatcaagg ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt
     8221 caaaacagcg ctaacagcga aacttctgca ggaacttaat gaccagctgc gacgggctct
     8281 tgtgggaaaa agtggtcagg aagcactgca gatctatgca gaaacttatc gatcctttgc
     8341 tttttcaaat caagcagtgt atgagttact gatcagtgta ccgcatacca atgaagctgt
     8401 tttattagat ggaatccatg aaacgaatca gatcattttg cagatatttg aggcttttca
     8461 attaacgaaa gaagaaaaag ttcatcgaag cagggaactt cgtagtatga tccacggtta
     8521 tctttcctta cgttttttag gatactttac aaaagaacca acaatcgatc cagaagacag
     8581 ctatttttgg atgatcaata actttatcgc tactttgcct taaacaatca gattcatttg
     8641 taacaatttt aagatgttca ttaaatgacc cttttataag agaaaattct tgcaactttc
     8701 ttgaaaagtg actaagatga gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa
     8761 gtaattacca tgttcgggga caatgagcct tggtggtttt ttgaagactg ggaagaagat
     8821 attgaagaag aagaaacatt cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa
     8881 tggaacgaaa tccatcaaaa ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc
     8941 ttttggaatg acggagatga gcgctggtgt gaagagtgtg acgatgattt acagcaatac
     9001 aagggacttg cgttgttgaa aaatcatcag cccatcacag tagaaagcag aaaggaattt
     9061 tatgaaacaa ctaattctag cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct
     9121 tggtgctaat caaaagcaca aaaattatta tgaaggaccc aaggtgatcg ttacttgggc
     9181 ccttggtcat ttattaggat tgaaaatgcc ggaagatctg aataaggaat ggcagagctg
     9241 gcaaatggac acgttgccaa tgatccctaa aaaactagga atcaaaccac ttcctaaaac
     9301 aggacatcaa ttaaaagcaa tcaagcagct ggctcagcga aaagatgtct cagaagcagt
     9361 cattgcgaca gatgccggaa gagaagggga acttgttgct cgctggatct tagagtatgt
     9421 gcatttcaat aaaccagtga agcggttatg gatctcttct caaacagata aagcaatcaa
     9481 aactggattc aagcaactaa gacctgcgaa agaatatgat gcattgtatg aatctgctct
     9541 tgcccgggca aaagcggact ggctggtagg attgaatgtg accagagcct taactgtgaa
     9601 gtacgcggat aatttatcag caggccgtgt gcagacaccg acactagcat tagtgagaga
     9661 ccaagaaaag aaaatcgagc agttccgtcc acaaacgtat ttcacgatca cgttgcaagc
     9721 tggaaaagaa caagccaaaa tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga
     9781 agcacaaaaa cttgttcaat tgatcagtac aagcaaagga actgtcacag aagtccaaga
     9841 aaaattaaaa acggaatctg cgccattgcc ttatgattta acagagatcc aaagagaagc
     9901 caatcaacgc tatggctatt ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga
     9961 gacccataag atcgttactt atccgcgtac agacagcaaa tatctgacaa atgacatgaa
    10021 aagtacgatg aaagaacgat tgcaagcagt cagcgacttt gctcctgagg tcaagcagta
    10081 tttgaaaaac ggagccatcg tccaacagac aaaagtattc caagacagta aagtgaccga
    10141 tcaccatgca cttttgccaa ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga
    10201 gcgaaaaatc tatcaaatga ttgtctctcg ttttttagga ctgtttgctc aaccccataa
    10261 agtcagccaa acaaaagtta cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa
    10321 tcgtgtcgtc caagcaggat ggaaaggcga aactgaaact gagacggaaa cagtcaaatg
    10381 ggaaaaagga atgagaatca atcccgattt cgccatcaaa aaagaactat ctgcaccgcc
    10441 taaaccattg tcagaagcaa gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc
    10501 agctacgcgt gcagagatta tcgagaaact aatcaaatct gaactgatgg aacgtacccc
    10561 tcaaggactg caagtatctc ctaaaggtaa gcaattatta gtattggtaa atccctcttt
    10621 agtaacacca gatttgacag aaagctggga aaaagacctt gaagctattg ctgcaggaaa
    10681 gaaacaagca ggaaccttct tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga
    10741 gatcaaatca agcaaacaag aatatcaaga tttctcgatt acccaaaaga aatgcccaga
    10801 atgtggggcc aatctgcgcg agaaaaacac acgtgacggc aaaatctatg tctgcaccaa
    10861 ttcagattgc aactaccgca gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca
    10921 atgtcaccgg aaaatggaaa tcatcgaagg taaaaacggt gcttatttcc gttgtaaata
    10981 cgatggaacg acagaaaaat tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca
    11041 tgaagaacga cgcttgctga aaaaatatgc acaagcagaa gaaccagaag aaagcccatt
    11101 agcagcagca ctaaaagcag caatgaagaa gtaataagat atgaaagagg ttgtga
//
LOCUS       DACEMO010000088        10788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEMO010000088 DACEMO010000000
VERSION     DACEMO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.090"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(107..2233)
                     /gene="pnp"
                     /locus_tag="IW728_001677"
     CDS             complement(107..2233)
                     /gene="pnp"
                     /locus_tag="IW728_001677"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1025037.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2421..2690)
                     /gene="rpsO"
                     /locus_tag="IW728_001678"
     CDS             complement(2421..2690)
                     /gene="rpsO"
                     /locus_tag="IW728_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1025038.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            2941..3504
                     /locus_tag="IW728_001679"
     CDS             2941..3504
                     /locus_tag="IW728_001679"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1025039.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3757..4278)
                     /locus_tag="IW728_001680"
     CDS             complement(3757..4278)
                     /locus_tag="IW728_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1025040.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4281..5123)
                     /locus_tag="IW728_001681"
     CDS             complement(4281..5123)
                     /locus_tag="IW728_001681"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1025041.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5116..5841)
                     /locus_tag="IW728_001682"
     CDS             complement(5116..5841)
                     /locus_tag="IW728_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1025042.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            6017..6313
                     /locus_tag="IW728_001683"
     CDS             6017..6313
                     /locus_tag="IW728_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025043.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            6881..8473
                     /locus_tag="IW728_001684"
     CDS             6881..8473
                     /locus_tag="IW728_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1025044.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8486..9226
                     /locus_tag="IW728_001685"
     CDS             8486..9226
                     /locus_tag="IW728_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025045.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            9394..10743
                     /locus_tag="IW728_001686"
     CDS             9394..10743
                     /locus_tag="IW728_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1025046.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
BASE COUNT         2990 a         2158 c         2151 g         3489 t
ORIGIN      
        1 aaaaaaagga ctgtgacacg gcttttgtca cagtcccctt ttttcgaata attattgtcc
       61 ccctgtcacc ttcacgatca caagtacatt attcctatga attaaattat ttttcttcat
      121 ttttcgttga accatcttcg ttcaacattg cttttcttga cagatttacg cgtccttgtt
      181 tatcgatttc agttactttc acaaggatct catcacctag tttaacaaca tcttcgactt
      241 tattgacacg atcgtttgct aattgggaaa tatggacaag tccatctttt cctttgatca
      301 agttgacgaa tgcaccgaat ttttcgatac ggacaacttt ccctagataa acttcaccaa
      361 ctttgacttc tttggtcaaa tcttcgatga tcttgatagc ttttttgatc atttctgcat
      421 cagctgaagc aatacttact tttccatctt gatcgatatc gattttcaca ccagtttcat
      481 cgatgattcc gttgatcgta tcgccgcctt taccgataac atctttgatt ttagcaggtt
      541 cgatctggat catttcgatt tttggtgcat attggcttaa ttcttcacgt ggagcagcaa
      601 tcgttgaagt caattcttct aagatttcca tacgtgcttg ttttgcttga gttaatgctt
      661 cagttaggat ttgttctgtg attccttgaa tcttgatatc catctgtaaa gcagtgatac
      721 catcttttgt accggcaact ttaaagtcca tatcgccaag atgatcttcc agcccttgga
      781 tatcagttag gatcgtatag ttttcaccat cagaaacaag tcccatcgca ataccggcga
      841 ctggcgcttt gattggtaca cctgcatcca tcaatgctaa tgtgccagca cagatacttg
      901 cttgggaaga agaaccattt gattccaata cttctgctac taaacggatc gtataaggaa
      961 attcttcttc tgaagggatt acttgtgcta acgcacgttc acctaatgcg ccatgtccga
     1021 tttcccgacg ccctggagag cctgcacgtc ctgtagaacc aacagaaaat tgagggaaat
     1081 tgtagtggtg gatgaatcgt ttgctgactt ccacccctaa accatcaatg atttggtgct
     1141 ctcctagagg tgcgagtgta caagctgata aagcttgtgt ttgtcctcgt gtgaacaaac
     1201 ctgatccatg tacacgtggc aataagctga cctctgatga caatggacgg atttcatcca
     1261 atttacgccc gtcaggacgg atcttatcga tggtgatcaa ttcacgtacg acatcttttt
     1321 caagatcttc tgtgatttgt ttaacttctt tcaacaagtg atcttcatct tcatgacctg
     1381 caaatttctc agcataagct tcgcggatat cgatcttcac ctgctcgata ttatcttcgc
     1441 gagcaagttt ttcttcagtc attactgctg ctttcattgt agcgtaagaa gcatcaaaga
     1501 tttctttttt caatacttca tcaacttgca gtaaagtcac ttccattttt tctttcccta
     1561 ctgcttgaac gatttcttct tggaaagcaa ctaactcttt gatagcgtca aaaccaaaca
     1621 gcaatgcacc aagcatgtct tcttctgaaa cttctttcgc gccgctttca accatattga
     1681 tggcttgttt cgttccggca acagtcaatt cgatatctgt ttgttctgct tgttctactg
     1741 ttggattcaa gacgtactcg ccatttacac gaccaacttc tacgcctgcg attgggccgt
     1801 caaatgggat atctgagatt gatagtgcta aagaagaacc tagcattgct gccatagctg
     1861 gcgagcagtc tgtttctaca ctcatcacga tgttcgttac ttggacttca ttacggaagc
     1921 cttctgcaaa catcgggcga attggacggt cgatcaaacg ggcagtcaat gttgcttccg
     1981 tacttggacg gccttctcgt ttgatgaaac ctccagggat tttccctgct gcatacattt
     2041 tttcttcata attgatcgtt aatgggaaaa agtctgtatc ttttgcttct ttagaggcca
     2101 cagctgcact taagactact gtatctccat aacggactaa aacggctccg tttgcttgtt
     2161 ttgctagctg accgacttcg atttgtaagg gacggccgcc ccatgtcgtt ttgaatactt
     2221 gtttttctga cataacttct ccttttcctt ctcgtgaaag caagacgcta ctaaacaaat
     2281 gaaaactgcc gaaagccgtc ttttgagcag ctttcatttg gttagtagga gtccatctag
     2341 cttgatcacg gaattttact taattgatat acgagtaaaa aaagtgagat tcatgtgaat
     2401 cccactcttt ttgactaaaa ttaacgacgc aagcctaaac gtttgatcaa ttcgcggtaa
     2461 cgttgaacgt cagttttacg taagtaagct aacaagttac ggcggtgacc aacttttttc
     2521 attaggccgc ggtatgaatg atggtctttt ttgtgcacgc gtgcatgttc attcaagtgg
     2581 ttgatttctt cagttaatac agcgatttgt acttctggag aaccagtatc cccttcatgg
     2641 cgagcgtatt cgttgatgat ttcattcttg cgttcttttg aaattgccat ttctttcacc
     2701 tctttcgatt attcccctgc gactgagtaa aacgttggtg attcgcttaa ccaagtggca
     2761 ggtcatgtgc ctagagcaca cagctatact ttacctgaaa ccgggttgaa aatcaagtag
     2821 ttaatcatta agaaaaggta gttttcaaac aatcttacta ctacttttcc atttttgtgg
     2881 taaactataa tgcgaaaata gacttgaaac acagtctttt aagaaagggg tacatattcc
     2941 atgattacaa tggatgatat cattcgtgaa ggaaatccga ccttacgcga agttgcaaaa
     3001 gaagtttctc ttccattaag cgaagaagat atctctttag gaaaagagat gcttgagttc
     3061 ttaaaaaaca gccaagatcc aatcaaagct gaagaactac acttacgagg cggtgttggc
     3121 ttagctgctc ctcaattaga tatttctaaa agaatcattg ctgttcacgt accaagtcct
     3181 gacccagagg cagatggacc ttctctaagt actgttatgt ataatcctaa aattttgagc
     3241 cattctgttc aagatgcttg tttaggggaa ggtgaaggct gtttatctgt tgatcgagaa
     3301 gtaccaggat atgttgttcg ccatgccaaa atcactgtgt cttattatga tatgaatggt
     3361 gaaaaacaca agattcgttt gaagaattat gaatcgatcg tcgttcaaca tgaaatcgac
     3421 catatcaacg gtgtgatgtt ctatgaccac atcaacgatc aaaatccatt tgcactaaaa
     3481 gaaggcgtat tggtgatcga gtagagaatt ggcaggttcc ttcgatacta tgctacaaga
     3541 aagctttata aaaaagggga gtgctcaaaa gctgcacttc ccttttttat gtttctccac
     3601 taatcgaaat ttagatagac atctagaaat cgcaattcca aaaaattctg tgtattggat
     3661 acgcatcttt tcttattttt tcttatttct tccatatggg tgtttatctc agagatgata
     3721 gaatagtttc taggataaaa agtagcagct acgatgttat ccttcgtaga ggattcctgc
     3781 ttcagctagt ttttgtttga tgtttttcag catatgcttt tctgggtagc ggatcgtatg
     3841 cagatggatg ccgcctgtta attcggataa caaagtagca ttactttttt gataacgtct
     3901 cataaaggaa tcgatatctt gtggcgtttt gatgtgcaaa gttcctgcta actcaccgta
     3961 aaggggatgt tccacaatta catcaatgat ttcaccacca ttttcaacga ttagtctcag
     4021 ttcttcttct gtttgatctt ttccatgctg gacagcgatt ttactgatgt gcccagtatt
     4081 ggtttcttct gtttcgagga tataacctct tgctgttgcc acgatacttt tacctgcagc
     4141 tcgtagtaaa gcaatatcgc ctacaatgat ctgacggctg actccaaaag ttttcgcaaa
     4201 cttgctagca ctcactggtt tttctgtatt tgccaaggtt tgcatgattt gttttcgccg
     4261 tgtctctcct tcgatcacac ttattcctcc ttcattttgt tttggtattc gcgcgagtgt
     4321 ttcatcaaat caacatacgt atctgttatt gtttccttat atcccttatt tttcagcaga
     4381 ctaacaactt tatccaatac ttgctgctct ctttttgcat cataaactgc ttggttttct
     4441 tcttttttca cttgtcctat tttagttacc gtatccattc ttttttctaa taaagcaatc
     4501 aactctcgat cgatcatatc gatcttatga cgtaatgttt caagagactc ttctgttagt
     4561 tcttcctcta ctgtcttgat gatacgagca ggatttcctg caatcactac attgtcggga
     4621 aaagatttgg tcacaaccga acctgcgccg actactacgt tgtcccctag agtgacaccc
     4681 ggcgtgacga taactcctcc gcctaaccaa acattattac cgattttaat gggttttgcg
     4741 tactccagac cgctgttacg tttcactgga tgtagtggat gcgtcgccgt gtaaagctgg
     4801 acattcggag cgaacatgca gttatcaccg atttcaatcg tgctgacatc taaaaatgta
     4861 caattgaaat ttgcataaaa attctcccca acaaatatat tatatccgta atcaaaattg
     4921 ataactggct ccatatagat ttttttccct gtacggccaa aagtctcttt caacaattgg
     4981 gatcgcagtt ccgctgattc tgcttggttg atgatttggc tttgacttct tgcaaacata
     5041 cggtcggcaa ccagctcagg atcattggca aaataaagct cgcctgcaat cattttttct
     5101 ttttcggttt tgaattcagt cataatctgc tcctaattct tgtatttttt tgaaaacaac
     5161 cgctccgtct tcttgaataa acgtataagg ctccgctaaa taaacaggcg tttgattgat
     5221 tactaagatt ttggctgacg gctggcgaaa ctgtaccaga tcacaaaacg gatgaacttg
     5281 aaaactcgtt ccaacaatga cgatcagctc tgcttggctg acggcataag ccgcctgctc
     5341 catgactcca tcttgaaacc cttcaccata taacacgatg tctgggcgta tttgtcctcc
     5401 gcatgcttga tgtttgtcgc tgtgaagata ttctttccag tctaccgttt gttgacattt
     5461 tcgacaatga cattgataca aacttccatg aaaattgacg agttgcgggc ttttagcctt
     5521 tgcgtgcaaa ccgtcgatgt tttgggaaac aacccaaaca tttttttctt tcgctagatg
     5581 tgccatttcc aaatgaatga tattgggttt tgcttcagga tgataaatct ttttgacaaa
     5641 ttgatagaat ttatccggtt cattcaccat tgcttgatga ctcagcaagt attccggctg
     5701 ctccatcccg tggtagattc ctgacaaaga tcgatagtct ggcactccag aaggtgtgga
     5761 tacccctgca ccggtcaaaa atgtaatttg ctgataagct aatatctctt ttgctgcttc
     5821 gtctactgtt aattgtttca tgttttgtca cctcactttc cattatagcc catactcgaa
     5881 caaatttatt gttgaaaagt cagaaaaatg ggaataaaaa agtgttatgg gcatttgaac
     5941 aatttatcac aatgttcact gacaaaagaa tgaaaacgtg ttacaatact gctgtataaa
     6001 agtgaggtga atcgatatgt taccattctt tatttattgg ggcgttattc tggcttgtat
     6061 cgcctggtta gcgttaagcg tttatttctc tgttttttat cttgtacgca aagaaaatgg
     6121 aaatctttgg gcttttgctt tattcaacgt gattgccgca attgttttag ccattacatt
     6181 agccgtatat cgaacatggg gctggggaat cacccaatac tcaagtttga tttatctaat
     6241 attagccatt tatggcgtcg ttgttctttt gcaagcgatc ctaggacgtg agccaaagaa
     6301 agcggcagct taaatgatcg agatggaact ggacacagga aattttctgt tctagttctt
     6361 tttttattgt attacataca tttttgtgta taattatact atcgtttgca atggatagat
     6421 gattcacatt ttgattgttt tttcggtaaa agtaaagatt tctatattta tcagtatttt
     6481 ttcaaaaaac agcagttata gattgacaaa gcctctcgag tatcgtaaac tttttttgta
     6541 taaataactg aatacgagat gcctcaatca atgaggtaga ggtcgcgaga atcattaacc
     6601 ctgtggagtg aagcaaacat ggagaagcag gaatagggag gatcgccgaa atgtgcactt
     6661 ttttgcttgg agtgtatgtt gggacgcaag ttaagagctt gcggactgtc ttagcagtga
     6721 tgctaagttg cgctatgttt tgtgagaggt ttatcacctt atggaatttt agcggccttt
     6781 ttatatctat aaatcggtcg ctttttattt tttcagataa agctttaccg catttcatct
     6841 agaccgctcg tgtatcatat aatcagaaag ggagaaaaaa atgaaaaaaa atatggtttc
     6901 agttgccgta ctatttgctt ttgtcttctc actattcagt gtgggatctc tggcaaatgc
     6961 agatgaaaaa acaccgagcg atgagtttcg cgtaggtatg gaggctgggt atgcaccctt
     7021 caactggtct caacaaacag atgagaataa tgccttgcca attcaaggcc aaaatagtta
     7081 cgccggcggt tatgatgttc aaatcgctaa aaaagtagca gatggtttag ggaaaaaatt
     7141 agtcatcgtc caaacgaaat gggatggtct ggctcctgcc cttcaatcag gcaagatcga
     7201 cgcgatcatc gccggaatga gccctactgc tgaacgacga aaagaaattg attttaccga
     7261 tccttattac gaatcacaat tagtagtagt agttcaaaaa gatagcaaat atgcaaaagc
     7321 aaaaagttta gcagatcttt ctggagcaaa gattactgct cagttaaata cattccacta
     7381 tactgtgatc gatcagatac cagatgtgca gaaacaacaa gcaatggata atttctcagc
     7441 aatgagaaca gctttagctt ctggaatgat cgatggttat gtcagcgaac gtccggaagg
     7501 tgtaacggca actagtgtga acaaagactt ggaaatgctt gaattcagcg gtgcagatgg
     7561 cttccagaca aacccggaag atgttcagat ttctgttggt atgcgtaaag gcgacccgga
     7621 tatccaaaaa gtaaaccaga ttctttctgg catctctaac gatgaacgaa ccaaaattat
     7681 ggaccaagca gtcaaagatc agcctgctgc aactgatagt gaagaaaaac ctggcatcat
     7741 ttctgatttc actacgattt ggaaacaata cggcaatatg ttcttacgag gtgctggact
     7801 taccttgttc atcgcattga tcgggacagt tgtcggtact acacttggat tactgattgg
     7861 tgtcttccgt tcggtacctg aatcaggaaa tgcactgact cgcttcttcc aaaaaatcgg
     7921 aaatgcactt ctttctatct atgtcgaagt attccgcgga acaccgatga tggtacaagc
     7981 aatggtcatc ttctacggtc tggcactcgc ttttggcatt tcattgaacc gaacgattgc
     8041 cgctttgttt attgtctctc tcaatactgg agcttacatg acagagatcg ttcgtggggg
     8101 gatttttgca gtagatgaag ggcagttcga agctgcgcaa gccattggta tgacccatgg
     8161 ccaaacgatg agaaaagttg ttattccaca agttttacgg aatatcttgc cagcaacagg
     8221 aaatgagttt gttatcaata tcaaagatac agcagtttta agtgtaatcg gcgtagctga
     8281 tttgttcttc caaggaaact ctgcttccgg tgcgaatttc caattcttcc aaacatttac
     8341 gatcgtcggg atcatctact tagtcatgac ctttacgatc accagaatcc tgcgggcggt
     8401 agaaaagaaa atcgatggtc cttcagctta taccaagctt gaagaaatcg acaattcaaa
     8461 tttgcaggaa taaggaggaa gaatcatgaa tgaaaaaatt attgaaatca aacatctaag
     8521 aaaaagcttc ggtgaaaatg aagtattgaa agatatcagc gtaacagtca ataaaggtga
     8581 agttgtgacg atcatcggtt cttccggttc tggtaaatct actttcttgc gctgtatcaa
     8641 tttactggaa aaaccaacag gcggcgagat catttataat ggagaaaatg tattagctcc
     8701 taaatataat ttacctaaat atcgaacgaa tctaggtatg gttttccaat ccttcaacct
     8761 gttcaacaat atgaatgtct tggaaaactg tatgtctggt caaacaactg tcctgaaacg
     8821 agataaagaa actgccaaaa aagtagcaat cgaaaatctg gaaaaagttg gtatggatcg
     8881 ttatatcgaa gccaaaccag cacaattatc tggtggacag aaacagcgtg tcgcaattgc
     8941 tcgggcatta tcaatgaatc cagatgtatt gctattcgac gaacctactt cggcgttaga
     9001 ccctgaaatg gttggcgaag tgcttaatac gatcaaagac ttagcacata ctggcttgac
     9061 aatgcttatc gtgacgcatg agatggaatt tgcaaaagat gtatctgacc gtgtcatttt
     9121 tatggataaa ggtgtgattg cagaagaagg tacaccagaa gatatctttg tccatccgaa
     9181 agaagaacgt acaaaagaat tcctttcacg tattttaaat gcttaggatc aatagctgat
     9241 tgggacaaag agctgcggtg tttaccgcag ttctttttta tgaattactc ataaaaggta
     9301 aatttctgaa aattttggaa tcttagttgt gtttttcaga aaactctttt aatatggaag
     9361 taattaaact tttttagtta ggatgattta aaaatgaatg aagagcaaga actgcagcgt
     9421 ggactgaaaa atagacatgt ccaactgatt tctattggcg gagcgattgg aacggggttg
     9481 tttttaggtt ccggaaagtc tatccagcta gctggccctt cgatattgct cgcctatctt
     9541 attactggat gcatctgttt tttcattatg cgtgcgttag gcgaacttct gttatcaaac
     9601 accaataacc attcttttct ggattttgtt gcagaatatc ttggcaaaaa agcggctttc
     9661 atcactggct ggacgtattg gttctgctgg gtatcgattg caatggccga tttaacagcc
     9721 atcggaatgt atgtacgcta ctgggcgcct ggtgttcctc agtggcttcc tgaactgatt
     9781 gcattaggtc ttcttctttg cttgaatatg gtggctgtct cattgtttgg tgagttagag
     9841 ttttggtttg cattgatcaa agttgtagcg atcattgcct ttatcctcgt aggagcttat
     9901 atgatcctca cccactatac gacagatatt ggcacagcca gtatcacgaa tctatggtct
     9961 catggcggat tctttccaac tggtgcaaaa ggatttatct tagctttcca aatggtaacc
    10021 tttgcttttg ctgggataga attagttgga ttagtcgctg gagaaacaga aaatcctgaa
    10081 aaagtgttgc cggaagctat caataatatt cctatcagga tcatcttatt ttacttagga
    10141 tctctttttg tcattatggc aatctatcca tggaacagct tgaatccaga taacagtcca
    10201 tttgtagaag tattttcgga aattgggatc acgattgctg cttccttgat caatttagtc
    10261 gtattaagtg cagcagcttc tgcctgcaac agtgcaatat atagtacagg acgtatgctt
    10321 cgttctttag cgcaagaagg cagtgcccct aaaaaattca aaaagttaac aacacatcat
    10381 gtgccaggga atgctttgac attttctact atcgtcattt tcatttctgt tatcttgaat
    10441 tacgtgatgc ctagtgaagt attcacacta gtaagcagta ttgccactac ttgtttcttg
    10501 tttatctgga gtatgcttgt ctacacgcac atgaaatatc gtaaaagtat cctcggcaaa
    10561 aaagctcatt catttaaaat gccgttgtac ccatttagta attatctggt atttctttac
    10621 atggcctttg tgtgtgtcgt cttgttccta ggaaaagata cgagaatcgc tttattacta
    10681 acacctgtgt ggttcttgct tctattacta atctatcata tgaaatatga acgtaagaag
    10741 taagaaagta gttgtgacag aagtggtcag cttcgagaaa taagaagg
//
LOCUS       DACEMO010000089        10493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEMO010000089 DACEMO010000000
VERSION     DACEMO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.091"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..155)
                     /locus_tag="IW728_001687"
     CDS             complement(<1..155)
                     /locus_tag="IW728_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1025047.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAE"
     gene            509..1357
                     /locus_tag="IW728_001688"
     CDS             509..1357
                     /locus_tag="IW728_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1025048.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            1371..2444
                     /locus_tag="IW728_001689"
     CDS             1371..2444
                     /locus_tag="IW728_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1025049.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            2434..4605
                     /locus_tag="IW728_001690"
     CDS             2434..4605
                     /locus_tag="IW728_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAQ1025050.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(4717..6888)
                     /gene="copB"
                     /locus_tag="IW728_001691"
     CDS             complement(4717..6888)
                     /gene="copB"
                     /locus_tag="IW728_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1025051.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(6908..9094)
                     /locus_tag="IW728_001692"
     CDS             complement(6908..9094)
                     /locus_tag="IW728_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1025052.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(9094..9303)
                     /gene="copZ"
                     /locus_tag="IW728_001693"
     CDS             complement(9094..9303)
                     /gene="copZ"
                     /locus_tag="IW728_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1025053.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(9316..9756)
                     /locus_tag="IW728_001694"
     CDS             complement(9316..9756)
                     /locus_tag="IW728_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1025054.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9830..10303)
                     /locus_tag="IW728_001695"
     CDS             complement(9830..10303)
                     /locus_tag="IW728_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAQ1025055.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
BASE COUNT         2968 a         2224 c         1861 g         3440 t
ORIGIN      
        1 ctttccgcca aacgtttcag atgccgatcc actgttttaa catcgatttt cagttcttca
       61 gctaaatcag cttttcgcca gatcaaatct ttcattagat tgattagtga aaataaattc
      121 tgtgcatttt ttgataatag tttttgccat ctcatcgatc atttctcacc tgatttctaa
      181 acatattgtt tgatcaaaat gggttcactc ctaaaaagat aaaaaactcc ctttctattt
      241 atttaaatac gcaaattttc ctcattattt ttaattttac tcaatttaat cgtattctca
      301 tttcaaaacg aatgtacttt aaaaaaacat aataaaatca ctttgcttgt aggatgtatt
      361 cccttccatt acaatgaaat aaatagcaat tttatgagaa ggtgatgtga aaatggaaga
      421 aaaaatcgaa gaatccaaac aaaaaatcga tcagttgata caagaattag aaaaagcggt
      481 catcggcaaa cgttcaacta tccaattgat gatcgtggcc ctattagcag gtggtcatgt
      541 cttattcgaa gatattcccg gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc
      601 tgtacaggga accttcagca gagtccaatg tactcctgat ctgctgccta gtgatattct
      661 tggtttttcc gtatacaatt cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt
      721 taccacgatc ttgttggcag atgagatcaa tcgcacgact cctcgtactc agtcagcact
      781 tttagaagca atggccgaga atcatgcaac gatcgacaac aatacatacc ctctagacaa
      841 tcattttttt gtcttagcta cgcaaaatcc aatcgagtat gaaggaacat atcctttgcc
      901 ggaagcacag ctcgatcgtt ttttattccg actacaaatc ggttatcctt cttttgatga
      961 cgaacttttg ctacttttag ataaattcga aaaagtgttg gataacttaa atgaagtatt
     1021 gaacagttat gaaatagaag aaataaaaac aaatgtggcg aatgtctttg tcagtcccga
     1081 agtggcatct tatgccttgc agttagtgtc agcaacaaga agccatgctg ccatccagct
     1141 tggcatcagt ccacgaggaa gcctttcctt tattcaggca gcaaaagctt atgctttaat
     1201 ccatgggaga aattacgtca cgcccaaaga tttgcaggat ttagttcctt acgtgttttc
     1261 ccatcgcctg attttttacg atcgttcatt gaaggaagac gaaaaaaaac aaatcattca
     1321 tactcttgtt gcacaaattc ctgttcctgt gaggtagatt gaggtagata atggaaaaaa
     1381 tcaaagatta tttcattaat tttgtgttat ttctggtata tttcatcatt gtgctttttg
     1441 ctcttgtatt caacaattct ttaggctggt tcttgttggc cttttttact ctttatgtat
     1501 catggagctt gatttcatta agcagctttc taaagaaact ttcggttact tgtacattgc
     1561 agccactcgt ttatcgcaaa aaaagtatgc aggttatttt ttccatagca aaaaagagaa
     1621 ctttttcttt acctctccct cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa
     1681 aacaagagat tttgattctt acaaataaac cgcaagaagt ccaattgctt tggcagccaa
     1741 aagaaagagg atgccatgaa acattaactg tcatttttac agcctatgat agtctaggac
     1801 ttttcagaaa aagtgcaaaa agagagatct cttttcctgt gactgttctg cctgcttttt
     1861 gtaagattca agcagaaaaa ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt
     1921 accaaagagg tcttgatttt cgggaacatc gcgcttatcg tccaggtgat tctttcaacc
     1981 gaatcgattg gaaattgtct gctcatactc aagagtggtt ataccgtgaa tacgattatc
     2041 aagaagaaga atgttctcct cttttttgct tttggggttc gcacagtcca aaatttgaac
     2101 ctctgctaga cttgtatttt tctttatggc atttgagaaa agaaaagcag ccggaacttc
     2161 tgatattagg caaccgcgcc tttcatggca aagaccccgg tcatacctat tttgcttctt
     2221 tggaagctgg cgatgagaaa gcttttttcg cacacctaaa aaaatatgca aaaaaacaaa
     2281 tcgtcctctt tgttcctgag aattctcctg aagtctcaga agctattgaa acattgtcaa
     2341 aagatcgaac ggtttatctt gtttatttcg tagaaaaaca actcatggtc caagaaaaag
     2401 acaagatatg ttcattgcca ggaggtgaat ggattgacga ttaaatggga aaaatggctg
     2461 tgtggcgttt actcctttgt gatactaagt gtaggtgctc cgatatttgt agtagctaat
     2521 caattgcaaa acagctacct tcttatccta actattggtg tcctatgcct tattgtctgc
     2581 tgtattccta aaaaatgggt acgtttgctg ctttctttgc cagtaatgat aggttgtttg
     2641 tttttgtatt ttccttcgaa gacattttcc ttattatgga tcttacagtt tttttcgtca
     2701 gtcacagagg aattcttcca tctatcccaa ggtaaacttt attatttgcc agaaaagaca
     2761 gctttttttc tcataatgtt gctgatctat ttatttatta cattgaccgt ggattacgac
     2821 tatttgtacg caccctttct ttctttgatg gcttacttca tgatgcttac cgtttttcgg
     2881 gaaaaagatt tactgtacga aatgattgcg gtagtaactg ttttgttcgt gtatctggca
     2941 gcaaggcaat ttttctccat gcggttgttg tcgggaaaca ctcgattcca gtcattgact
     3001 acagttctgc ttatttgttt cttaactatt agcgctatct tgcctttata tcttccgaaa
     3061 gtacaccaat ctctcgagga aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg
     3121 ctttatgata cactccatga gtatcaatta accggaagtg caagaacagg cttcagtgag
     3181 aatgatgaac agcttggtgg acctttatat gataatggcg aagttgtttt tacagcgaac
     3241 caaaaaggaa atggctattg gaagatcgaa aataaaaatc attacaccgg ttctggatgg
     3301 atagaagatc aggacgcacc catccaacca atccaagaat tccctgttga catcacgagc
     3361 taccaaaccg ttatggaaga caaagaaaac attacattga ccctaaaaaa acaagctttc
     3421 ttaccttatc cttatggaaa tactaccttg actgattttt cattagctag tggacaaacg
     3481 ctttactatt tgcctgaaag ccagcgattc attcctgtcc cgaatgaatc gacacgttct
     3541 gcaactttac aaacagaaag gttagttttt acgagagaag aattaaaaag cagtcagtta
     3601 aaagctgatg atttagaaaa tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa
     3661 cgtattgagg acttggcggt cgaattgacc aaaaatgaag caactttaat tcgtaaagtc
     3721 gaagctgtcg aacagtattt aaaaacaagc ggaaatttcc gctattccaa aacggatacc
     3781 ggacatactc cgaaagaaca cgattatgtg gattatttcc tctttgattc aaaagtcggc
     3841 tattgtgaca acttttcaac ttctatggtc gtccttcttc gaacacttgg tatcccagct
     3901 cgctggacaa aaggttttac aaatgggctc agaacaggca cagataatca agggaatgag
     3961 gaatacagca ttctcaatag tcatgctcat tcttggccag aggtatactt tcctggattt
     4021 ggctggcttc catttgaacc aaccccttca tttgccaatc caatgggttc acaaccagtt
     4081 gctgaaacaa ccgaaaccgt ccagcctgct atcgaacaac caaatgaaag cgaaaatatg
     4141 gcagaaacta tttccagtga gagatctgag gtcattccat cagaagaaac aacactcaca
     4201 ccagcttata aaatcgtctt attcagcctt ggggtgtgtt tatacgtctt gttgttcttt
     4261 ctattgttat tcagaaaatc tctgcgctgg acaagttatc ttatctatct tcattttttt
     4321 gcttctacta atacgattgc atatcaaaaa cttcttaacc atttggaaac attggtaccg
     4381 cgcaaacccg cagaatcttt gcaggaatat agcctgcgaa tagaacaaga attcaagcta
     4441 gatctgcgtt tcattcgatt aactactcaa tatgaagcag aaatatatgg acgacaagca
     4501 gatccggtaa atttcgataa aaaaacctat ttagaaatta cgcatataat aagaaaaacg
     4561 gtatacaaaa aagttctgac aaaagggagc cgcttcaaaa aataagataa aatttctgga
     4621 gaatctactt ctggaatgcc gagaaccagc tgcttgagta tcgattattc gaaaaaaagg
     4681 ttgtgacacg ttttttgtca caaccttttt tcattattat ttgattttca atgtcaaagc
     4741 atttagagct acaactaccg tacttaacga catcaagaca gcaccaactg ctggacttaa
     4801 tatgatacca attggcgcaa gaattccggc tgctagcgga attgcaagaa tattgtaacc
     4861 tgccccccac cagaggttct ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa
     4921 atgcaaaata tcttttggat cgctgtttgt caatacgaca tccgcagaat cgattgcgat
     4981 atctgtccct gcaccgattg ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt
     5041 gatcccatca cccaccatga ttactttttt cccttggtca aggtatttct ggacaacagc
     5101 ttctttatca tctggtaata aaccgccata atattcttcg atccctaaat aatctgctac
     5161 tgcttgggca gcgtttttat tgtcaccagt aagcatgaca ggagtgattt ttctttcttt
     5221 caatgttcgg ataaattctt ttgcttctgt ttttactacg tcacctaaag caacgattgc
     5281 agctagatga ttttctaaaa tcaggaagct gatggtattt ccttgttctt ggtaaggttt
     5341 caataatgct tgttctacgt ttaatccgag acgctcaact tctttttcat taactatttt
     5401 cacttgttgg tttttgactg ttgcctctag cccaactcct gaaagatttt ttagattttc
     5461 ggcttgataa ggcttgattt catgttcttt caaataattc ataataccaa cagccaatgg
     5521 atgattggca ttcgcttcca atgctcctaa atattgaagg atttcatttt tattgaacgc
     5581 atcatcaagt acttcgacac ccgttacagt aaattttcct tctgttagtg ttcctgtttt
     5641 gtccagcatg atcacatcta gatcattggc ttgctccagt gcattgcggt ttttcaaaag
     5701 taatccgttt ttagctgcta ttgatgtaga gcgagcaaca actaatggga tagctaaacc
     5761 tagagcatga ggacaggcga tgataaagac tgttaccata cgttctaatg cattaggaag
     5821 atctgtcaag aacaaccagg cgatgaatgc caggataccc acaattaatg cgacataaaa
     5881 cagccatttt gccactttgt cagataatga ctctagcttg gatttttctc cttgtgcttt
     5941 tcgcaccatt tccataacct tcgctagata accattttca ccagttccag ttacttcgat
     6001 ttcgatcgtt ccatccccat tgatagaacc gccaatcact ttgtcattgg cttgtttttt
     6061 gattcctctt gattcgccag tcacagctga ttcgtcaaca attgtttcac ctttcaagat
     6121 tacaccatct gttggcattt tatcaccaga ccggacgatc aaacgatcac cttcgtttac
     6181 ttctttcaat gaaactgtct cttcttttcc atctttcgtc aagcgtttta ctgattctgg
     6241 cagtaattct gctagttttt gtaaagcatt gctggcattc gacacagcat tcatttcaat
     6301 ccagtgacct agtagcataa taacgatcaa agtagccaat tcccagaaga aatccatgac
     6361 gtgtgtatgt ggattgagca gatttgcgat aaacgaatag acactataaa tatatgcaac
     6421 tgtaatcccc atcgcgatca gtgtcatcat agcaggactt ttttgtttca attccatctt
     6481 agctccactt aaaaatggct gaccgccata gataaatagt atagttgcta agatcaatac
     6541 gagccaatca gaaccaggaa acgttacttg aaatggaaat tccattccca tcattggtga
     6601 aaaaagaatg attgggatag ctaggataag cgatagccaa aatttttgtt tgaaatttcc
     6661 catgtgcatg gaatgatcca tgccgccatg atcgtgatcc atgtgcatcg aatggtccat
     6721 ttgtttgctg tttgctatat ctccatggtt atgtcccatg tgcatggaat ggtccatctg
     6781 gctatggtca tgtttcatct ccatctgttt ttcatgagat tctgtacttt ctttgtttac
     6841 tacttctttt ttatgttcat gttttttctc catatcgttc ccattcattt ttactccatc
     6901 ccttctctta ctttttcatg tgccgattta agctcaggga gttcaacaat acactgacgg
     6961 aactaaacgc catcgctcct cctgcaatga ttggattcaa aaagcctagt gcggcaaaag
     7021 gaataccaat cgtattatag acaaaagccc agaaaagatt ttgtttgatt tttctcaatg
     7081 tcaccctaga caactcgatt gtttgtgcaa tggatgccaa actgctactc atcagcgtca
     7141 catcggctgt ttccatcgcg atatccgttc cacttcccat tgccatacca acatcagcta
     7201 atgctaaagc cggtgcatca ttgattccat cgcctgccat accgactttt ttgccatctt
     7261 tctgaagttt ttctacgtag gctgctttat cctctggaag tacttcagca aagatatgct
     7321 ttggatcaat accaacttgt ttaccaatcg tttcggctgc cagtttgttg tctcctgtca
     7381 gcataaaaac atctaatcct ttattctgga gctgcttgat tgcttgtttg gcttctagtt
     7441 tgatttgatc agccacggca atcaacccga tcacttgctt ttcatcagct aaaaacatga
     7501 ctgtttttcc ctgctgttct aattctaacg cgtgttcttg gtattcatca aatgataggt
     7561 tcaattcaat taatcgttta cgtgtacccg caaaatagcg tactccgtta atcgtaccgc
     7621 tgattccagc acctgggtga gctgtaaaat caatcatatc gtatgttgct gcttcttgca
     7681 gttttccgta ttccacgatt gctttaccta gtggatgttc agaagcttgt tctaaagtat
     7741 aaaaaataga taggacttct ttagaaccga ctagatctgt aactttgggc tttccttctg
     7801 taatcgtccc agttttatcc aaaacaatgc tgtctaagtg agcagctgct tctaatgctt
     7861 ctccgccttt aattaaaatt ccatttcgtg caccaacacc cgttcctacc ataatagctg
     7921 ttggagtcgc taaacctaat gcacatggac acgcaatgac taatacagag acactatgga
     7981 tcaatgcgag ttgccaatca ccagtgatca aacctgtagc tatgagcgtg ataaacgcga
     8041 tgactaatac gatcggaaca aaaatccctg agatccgatc tgcgattttt tggattgggg
     8101 ctttgctccc ttgagcatct tccaccattt gaatgatttg cgctaatact gtctgcttgc
     8161 cgacttgtgt gacttccatg tgtagcagac cattcgtatt gattgtcccg ccaaaaagcg
     8221 tgtcatccgg attcttttct acaggaagat tttcaccagt aagcatactt tcatctatcg
     8281 cactgcttcc tgaaataatt cgaccatctg ctggaatctg ctcaccagga cggatgacta
     8341 gttgatcacc aacgactact tcttcaatcg ctaacgtttc ttctttcccg tttcttatca
     8401 cttgggcggt ttttgtttga agagacatca attgtttgat tgcattacct gttttcgttt
     8461 ttgctgtatg ttccaaatat ttgccgagca aaatcagcgt gatgatcata ctgctgcttt
     8521 caaaataaag atcttggggg tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg
     8581 cagccgatgt tccaattgct accaatacat ccatatttgg tgctttcgac ttgattgcat
     8641 ggtacgcacc tttataaaaa cgagcaccga cataaaattg gacaggagca gacagcacta
     8701 actggacgat tggaaaatga aaaaagtgaa caatcgccgc atggcttcct aacatcatag
     8761 caatcattga caacatcaac ggcaaagtca aaaccgtact caatataaga tcgaatagca
     8821 tttttttcaa atacgcttgc ttttcttctg caattttttg tttgtgggct tcatcgtaca
     8881 aaattgcacc atagccgatg ttttctacgc tttggattaa tttttcagtg gtcgtccctt
     8941 catatttgac acttgctttt tcagtggcca agttgactgt ggcattgacc acaccaggtt
     9001 gtccgttcag ctctttttcg atcctagcag aacaatttgc acaagtcata cctgtaatga
     9061 cgaacgtatc cattttggtt tctgctgttg ccatcaaatc acctctgctt tataacccaa
     9121 tccattgatt gcttgacaaa tcttttcgga ttgaactttt gtttcatcaa acttgaccgt
     9181 accgtttgct tttttcaaat ttactttcac tttttgcacg ccgtcaagtg cgcttactgt
     9241 ttcttctact cttgctgcac aatgattgca gctcatacct tcgattgaaa attgttgttt
     9301 cattttttct tcctcctatt gatgttgttt acattcacat tgacctggga tacagttaca
     9361 ttcgatcgta tctactggtt gcttttgttc cagttgtttc aaaatctcat tgatatcttc
     9421 ttgagtcaaa gtggcttctt ctactagatc cgcaatcgtc tctccaatcc tttttgcaca
     9481 gatatgggaa aaaagatttt ccgtcgcact gcgaactgtt tccatctccg agacagctgg
     9541 atgataaatg aatttttttc cttcttgttc tgtccagatc acttcttttt ttaccaaacg
     9601 acccagcaaa gttttgatcg ttgctggttt ccaatccatc gaatcagcta agatctgcgt
     9661 gatttcttgg gctgtcgtct gtccaagtgt ccaaataaca cgcatgatct cccactctga
     9721 atcactaatc tttactggcg ttttgacttt ttccatttat ttcacacctt tcgtttacaa
     9781 atgtaatcat taacttatga atacagatta cacttgtaaa caaaaactgt caattatttt
     9841 tgctctctat tttcaattta gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga
     9901 tcagtttatt tcttgcatta gctacttgga tcagctgctg tatttttggt tcattgtttc
     9961 cttcaaaatg ccagataaaa gataaaaatt ctaagtattt cctgtttagt ttctccgaaa
    10021 atgattcagg catatctggc cgagattttt tgtttatctt tctttttaga gaacgccaaa
    10081 ctactcggta gatggcgaga taacgcggca ctttaagcca aatgatctta tctgcttctt
    10141 taatcctttc ctctaacgta gaagtataat ttccttcaac gatccatgac ggattagata
    10201 ccataaacga ttgctgttct tgcaaaaacc acttttttgc ctgaatgcgg aaagccggtg
    10261 atttctttga tcttccttgc gagggacgat tttcccgacc cactttgtcc aatcacgatg
    10321 atcttcattc ttctccctca cttctctttg gaatttggtt cgatcatacc acttataacg
    10381 ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaataaat
    10441 ggtgttcaat cagctgacct tcagcattag ctcaaaaaac ggcaaaatat gag
//
LOCUS       DACEMO010000090        10339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEMO010000090 DACEMO010000000
VERSION     DACEMO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.092"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            176..325
                     /locus_tag="IW728_001696"
     CDS             176..325
                     /locus_tag="IW728_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1025056.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            336..1850
                     /gene="dltA"
                     /locus_tag="IW728_001697"
     CDS             336..1850
                     /gene="dltA"
                     /locus_tag="IW728_001697"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1025057.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1847..3052
                     /gene="dltB"
                     /locus_tag="IW728_001698"
     CDS             1847..3052
                     /gene="dltB"
                     /locus_tag="IW728_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1025058.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3097..3330
                     /gene="dltC"
                     /locus_tag="IW728_001699"
     CDS             3097..3330
                     /gene="dltC"
                     /locus_tag="IW728_001699"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1025059.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3333..4601
                     /gene="dltD"
                     /locus_tag="IW728_001700"
     CDS             3333..4601
                     /gene="dltD"
                     /locus_tag="IW728_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1025060.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4742..5824
                     /locus_tag="IW728_001701"
     CDS             4742..5824
                     /locus_tag="IW728_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1025061.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5824..6627
                     /locus_tag="IW728_001702"
     CDS             5824..6627
                     /locus_tag="IW728_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1025062.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6697..7437
                     /locus_tag="IW728_001703"
     CDS             6697..7437
                     /locus_tag="IW728_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1025063.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7448..8452
                     /locus_tag="IW728_001704"
     CDS             7448..8452
                     /locus_tag="IW728_001704"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1025064.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8497..10260)
                     /locus_tag="IW728_001705"
     CDS             complement(8497..10260)
                     /locus_tag="IW728_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025065.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3330 a         1704 c         2088 g         3217 t
ORIGIN      
        1 attttttaaa agtacgagaa attataaaga aaaaagcagt gatttgatgt gctattttta
       61 taaaagctat gtgacaaaac tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt
      121 tctttttgtc aaaattagtt acactttgtg tgaggttata tgaggaggga atccaatgaa
      181 caaatggcgt tctaatccaa aattgaatta ttggggaaaa ttcatcattc gaacaatttt
      241 ttatagtgca gtactgctgt tcctgattta tctgtatcac tataaaaata ttcaaggagg
      301 aacttttatc tataacgaat tttaggagga agagtatgga aatcaaaacg attattgaag
      361 caatcgatga ttggggaata aatgaacctg atcgagttgc ttatcaggcg gatgagacgc
      421 atacttttgg tgaattgaaa caagcatcag acgcacttgc ttattattta gaaaaaaagg
      481 tagaaggcga tggaccaatt gtcgtttttg gcaatttgga gtttgaaatg attgtctcat
      541 ttctaggggt tgtcaaatct ggccatgcct atttgccgat tgaagagcat acacccaagg
      601 agcgtatcct atcgatcttt cgggtggcta aaccatctat ggtcatcagt atcggggact
      661 ggatagaaga tattccttcg gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga
      721 agataccagt aggattttat cccagaaata gcgtaaaagg tacagaaaat ttttatatta
      781 tttttacttc tggtactact ggagaaccaa aaggcgtaca aatcagtcat gataatctag
      841 tcagctttgt tcaatggacg atggaagact ttgggatcaa gcctggtatg catttcttag
      901 cccaagcgcc attttctttt gatttatcgg tattttctat ttatcctgca ttagtttctg
      961 gcggaatgct gaagccgtta aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga
     1021 cattgccagc cttgaagttg aatgtgtggg tttctactcc atcatttatg gatatttgct
     1081 tgatggaacc tacattcaat ggagaaaatg tgccggaatt ggacatgttt ttattttgcg
     1141 gagaagaatt gacgaaaaaa actgcagaaa gtctacttga tcgattccca aatgcacgga
     1201 tatataatac ttacggtcca acagaagcaa ctgtagctat ttcaagtatt cagattgacc
     1261 agcatgtact agatagttat gaccgactac cgattggcta cgtaaaaaaa gacacgaaag
     1321 tttctattgt ccaagaaggt aaacctgttt caaaaggaga aacgggtgaa atcattattg
     1381 caggaccaag cgtatcaaaa ggttacctga acaatcccga aaagacagct gctgcgttct
     1441 tttgtcaaaa tgacttagca agttaccata caggggatgc aggtagacta gatgaatatg
     1501 ggcttttatt ttatgaagga cgtatggatc agcaggtcaa attacatgga taccggattg
     1561 aactcgggga tatcgaacat tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg
     1621 taccaaaata ccaaggaaca aaagtacagc agctggttgc ttttgtcgta ttagaaaaca
     1681 aaacgaaaca gccggatttt cagttgacta aatctatcaa agaacaatta ctagaagttg
     1741 taatggatta tatgattcct cagaagttcc attatgtcga tctgctgcct cagacagtta
     1801 atggaaaaat cgatcgaaaa aaattaacag ctgaggtgaa tcctgaatga tgttacctca
     1861 tatgatccct tatgcagagc cgttttattt ttttctttta gccattgcgc ttgtaccgat
     1921 cgttctatct ttattgatat gggaaaaaag attaccagtt tatcaatcag cagtgacact
     1981 tttcttttta tttgtcagct ttggaggtaa ttattggcaa cagggagcgg cgctgattgg
     2041 gtatgttttg tggcagacga tacttgtatg gggctatttt gtttatcggc aaaggaaaaa
     2101 tcagacagga tggttttttg ccgccgttat tttagcgata attccactgt ttttaacgaa
     2161 agtgactcct ttttttgaac atggaaaagc atcgttactt ggttttttag gaatctctta
     2221 tttaaccttt aaatctgtac aaatgatcat ggaaataaga gatggcatga tcaaaacata
     2281 taagccattt cgttatatcc agtttctgct gttctttcca acaatctctt ctggaccaat
     2341 cgatcgctat aggagatttg aaaaagatca gctccatccg cctgttcgtg ataaatatgt
     2401 tgatcttttg ggaaaaggcg tccaaaatat ttttgtcggt tttttatata aattcattat
     2461 cggttattat tttggtcaag tactgcttcc tgtagtggga cgcattgcct tacagcatgg
     2521 tggtatttca tgggcattag taggttatat gtacgtgtac agtatgtatt tgttttttga
     2581 ttttgctggc tatagcttat ttgcagtcgg tacaagctac atgatgggat atcagacacc
     2641 aatcaatttc aataaacctt ttttatcatg gaatatcaaa gaattctgga atcgttggca
     2701 tatgacgctg tccttttggt tccgtgatta tgtatatatg cgattgatgt tcttcatgat
     2761 gaagaaaaaa ttttttaaaa gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt
     2821 tcttctaatg ggtatatggc atggattgac gtggtactac atcgtatatg gactgtatca
     2881 tgcttgtctg atctgtctga cagatgcatg gatccgattt aagaagaagc ataaagagca
     2941 gttgccttct aacaaattta cgcatggctt agccgtattt ttgacatttc aagcagtatg
     3001 ttttagcttc ctgatttttt caggattttt agataaactt ttttttagct aacgaatagt
     3061 gacatattaa tttatataac aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc
     3121 ttagaagaaa taacaggaac agatgaagta agaaaaaatc gtgatattga tctatttgat
     3181 gaagggttgc ttgattcttt agcatctgtt caattattag tcgagctaga cggagagctg
     3241 gatatccaag tccctgtatc tgaattcgag cgagaggatt ggagcacacc gaataaaatc
     3301 attcaacaag caactgcatt gaagggttga gtatgaaaaa gaaacttttt gctattttcg
     3361 gcccggttgt tttagctgtt ggcttactgt ttgtgttttt tacctcctcc tatcaagtag
     3421 atatctctaa tccagaaacg attagacgtg cctcttcttc catgtcagtg aacgtgttga
     3481 aaggagatgc gataaaaaat tatgcattat ctgaaaaaca atatattcct ttttttggct
     3541 caagtgaatt aagcagaatc agtccatttc atccgtctgt tctggcagaa aaataccagc
     3601 gaaactatcg tccatttttg ctgggagcac ccgggacaca atcgttaagc caatatatga
     3661 tgatgcgttc tgcaggagat gctatgaaaa acaagaaagt cgtctttatt atttctcctc
     3721 agtggttcgt taaaaatggt gtaaaaactg attactttaa tacgtattat tctgaacttc
     3781 aaacatacga ctggttgttt tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta
     3841 gaagattatt aactttctca aaagtaaaag agaatgatac gttgacggcg attctgcaaa
     3901 cgatcaaaaa agggaagcta cctttacctg aatctttgaa tcagctgcgc agccaatgga
     3961 acatgctgaa gagagaggat gaagtattca gcaatattgg gttgaaagat cgacagcaaa
     4021 aaatcgatca tgagtcaaaa cgattaccaa aacaatacca agaaacagaa ttatccattt
     4081 tagcaaatca aattggagaa cgagaaacga cgaataatcc attcgggttg aaaaacgatt
     4141 tttatacgca tcgtatccga gcccacgagc cagagctcaa acagtcacaa aaaaattggg
     4201 attatcgttt ttcgccagaa ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa
     4261 accataacga agtattgttc atcatcccac cggttaacga gaaatggagc gattataccg
     4321 gactatctca agaaatgtta caaggatttg ccaaaaagat caaatttcaa ttaaatagcc
     4381 aaggattcaa tcgaatagct gattttgtca atcaagcggg taccaactat tttatggaag
     4441 acacgatcca tctaggctgg aaaggctggt tggctgcaga tcaacagatc agaccgtttt
     4501 tagaagaaaa tcatattact gctagtaagt atcatcttga cgatgctttt tttagcaaat
     4561 cttggcaaca tcaaatacca gataaactac aattaaaata aagaaaacag gcgagtgttg
     4621 ctcgcttgtt ttctttattt ttgaatattc tgaataaaag ataaaaatac caagtttgcc
     4681 tgaacaaaaa tcataagcaa tggtataatc acaatatgaa tatagaaatg ggggaaaaca
     4741 tttggataca aaagaagaac agctacttat tgatttaact tctgcaaaag gagtacctgg
     4801 aaatgaagaa gaggtaagag aagtattcag agaatatgcc aaaccattcg ctgatgatat
     4861 tttttatgac ggtttaggca gtgtgattgc caaacatggt gctggcggag gccccaaggt
     4921 ctttatttct ggacatatgg acgaagttgg ttttatggta acaaaaataa ccgaaaaagg
     4981 attcttggaa ttccaaactc ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt
     5041 tgaaatcaaa acacgtgaag ggaaaatcgt ccacggtgtg attggttcca aaccaccaca
     5101 tgttttaaca cctcaagtaa gaaataaacc ttatgaaata aaggatatgt tcattgatat
     5161 tggcgcatca agccaagaag aagccaaaga gtggggcata cgtcctggag atatggtcac
     5221 tccatatatt gaatacaaac gaatgaatgg cagtaaatat ttactggcaa aagcatggga
     5281 taatcgtatc gggacagctg tttctctgcg agtattagag aatctatcta aagaagcaca
     5341 cccaaatgtt ttattcgcag gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg
     5401 aacaagtaca caccttgtaa atcccgatat tgcttttgct ttagatactg gtacagcagg
     5461 agacactcca ggaatgacac caaaagaagc agattcgatt ttaggaaaag gcccacagat
     5521 tttgattttt gatgcatcta tgatcccgca taaaaaatta ttgaattttg ttattgatgt
     5581 agcagaggaa atggatattc cattccaata tacagtgatt tctggcggag gaactgatgc
     5641 aggacaaatg catttgacaa gagatggtgt tccgtctgta gccattaccg ttcctgttcg
     5701 ttatctgcat tcccatacat cagtgattca tgaagatgat tatttgaata cagtgaaact
     5761 agtcacagaa ttagtgaaac ggcttgatag tgaaacagtt gaaaaaattc ggagttatga
     5821 ataatgaata atagaatacc tatttctgaa tatcggaaaa aagcacgagt ttctttggaa
     5881 aatcaatggg gaatcaatac ttggcttgtt ttcttatcta taatatttac gagcgtcatc
     5941 cagaactttt ttgacggttt cagtcctttt ccggaagaaa gcatgcagca gcacttattg
     6001 agttttctag tcgataattt acttttattc gcattcaact atgcattgtt ttacattgcg
     6061 ctagtagttg tacggggtgg cagaccaaaa agtaaccttt tattttcagt tttccaaaga
     6121 aagtattttg gttcgatact tggcatcaat ttgatcaatt cagtcatcaa ctggctactc
     6181 aacagtttaa tctttctgcc catgttttta atcagcggag tgagtactta tatgcagctg
     6241 cttttcagtt caaatgcggt acctacgggt gtcatgccag aagattttct tgatatcagc
     6301 tttgttttat cgatgatagg gatgattttt gtttcagcac tcatcatgag tattgttggc
     6361 ggtttattcc aatttgctgc atggacaaag ttcgactatc ctgatttgtc ggttattcaa
     6421 tgtttgaaat atggctggtt tttattgaaa gatagaattg gtacgtatat tttgttgcag
     6481 ctgtctttta tcggctggta tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg
     6541 gtcattgttt atgtgaatgt gtcagttgca gaattttatg aacaagcacg gattgaaaaa
     6601 ggaggtccag acgcctattt tacttagcat gtaagcagtt tattcatcaa aaaaataact
     6661 tttgtggtag gataaatctt tgagggggaa tgtacgatga aattaactgt tttaggatgc
     6721 ttaggagcgt atccttataa aaatcaaggg acaacaggtt atttgcttca atctgacggg
     6781 ttcaatttgt tgatcgatgc aggtagtgct acgctgatca aattacaaga acaccttgat
     6841 ccacttgatc tagatgctgt gatccttagc cattatcatc atgatcatat tgctgacctt
     6901 ggtgtattac agtattactg gcaacttcat cctaagagaa atccggagac attgctttca
     6961 atttatggac acacaaaaga ttccattcac tttgaagaat tgacgttgga aaatgtctca
     7021 aaaggacatc cttattttga agctgaagaa ttaaaaattg gtccttttct gattactttt
     7081 atggaaacaa tccatccagt agtatgttac gccatgcgta tcgtagaaga aacaacgggg
     7141 aaagtattcg tatttacagg tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa
     7201 gatgctgatt tgtttctagc agatacctat ctttttgaag gaaatgaaca tcatcatgcc
     7261 catttcactt caaaagaagc tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta
     7321 cttacacacc tacctcagtt tggtgattta gaacaattga aaaatgaagc gcaaaaggca
     7381 gcaggcaaag ggattgatat agaacttgct gcagttgaca aagtgtttaa catttaggag
     7441 gaagaacatg atttttgtac caaatgaaaa ccacgaccca agaatcaatt tagcaattga
     7501 acaatatttg ctgcaagaaa tgcctttaga tgaacctatt ttgctctttt atatcaatga
     7561 accttcaatc atcatcggca gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt
     7621 ggaagaaaat ggcattcatg ttgtccgtcg cttaagtgga ggcggagctg tctaccatga
     7681 ttttggaaac ctgaacttta gttttatcat gcctgatgat ggggattcat tccgtgactt
     7741 tgcgaaagta acacagccaa tcatccaagc acttcatgaa ttgggtgttg ctggggcgga
     7801 attgaaaggc cgaaatgatc tagtcattga tggcatgaaa ttttctggca acgcgatgta
     7861 tgctaccaac ggtcggatgt ttgctcatgg aacaattatg ttcgactctg atatcaatga
     7921 agtagtcaat gcattaaaag taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat
     7981 ccgttctcgt gtaacgaata tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac
     8041 agaagaattt cgtcaagaaa tcttgttgaa aattttcggc gtagagtcag tagaagaagt
     8101 aaaaacgtac gaattgactg atgaagattg gaaaaaaatc aatcgaatct ctgaggaata
     8161 ttatcgaaat tgggattgga attacgggaa gtctccagat ttcaatttct ctcgccaaaa
     8221 acgtttttct atcggttcga ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga
     8281 agcaaaaatc tttggcgatt tcttcggact aggagatatc tctgacgtgg aaaaaaaatt
     8341 ggttggacag aaatatgata aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa
     8401 gaaatatttt ggtgcgatag aaccagaaga attgcttacc ttactttatt gaaaataaaa
     8461 aatagcggaa tccacatata tggattccgc tattttttat acaggattcg ggtaatttcg
     8521 agagagttga acagtgatcc gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt
     8581 gtaacgatcc gctacgtctt tcatcatcat taagtcaaaa cgttcctgat catctaattc
     8641 ttctgtgatg cctcgactaa ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat
     8701 gacgaaatgt tcctcttgtt cgtgtatcga caaataaatt tttcgataca gtggattttc
     8761 gatggcatga gtttctctaa aggaatgctc taaagcgtgg atcaaaagtt gagccagatc
     8821 gtcatcgttg ataggaagtt cttcgggaag atctgcttgg aaggttatct gaatattccg
     8881 tttggcagct aacttctggt aacgcgctaa gataagattt gtcacctggt ggttgcaaaa
     8941 agaacacagc atttcgaaat tttttgcttc ttctaatatc ttttccacat agtttttagc
     9001 ttttgtttgg tccgtatgca ttaaatcttg cagattctcc atctcttgga caaattccag
     9061 gcgaagtgta tttacttctt ggatatgctg tcgaagctgc tcataatgtt gctccaacaa
     9121 atcatttttg atttttaaga aatctacttg tctccgatct ttgtcggtat ttaagatgtg
     9181 atcgattaga ttgtagctga taataacgat tagaatgata atatataaaa tcgtagacca
     9241 attgatcgtt gtttgataga tacctaatgc cgtttggatg taaatcaagc aatggataat
     9301 ggctaccaag acgatccaag gtgtacaagc tacataaaaa cggttattct tatatgcttc
     9361 gccaatgcat gcaagtgaag tgactaaagt gcttataaca ctagcaatat tagcaatcgg
     9421 cattaattca ggaagttctg caggagtaaa agttgcataa agcagaacgc cgataaaaat
     9481 caaaagttgg aacgtacaaa aaattccgta ccatttttta tagtaaaaca ttcggctcca
     9541 atagtagcag atgatgaaag ctgacgttaa gataataaat aaatgagata aaaaagaatg
     9601 aacgaatggt tcaaaaagaa taccacttaa aatatcttct gatacagctt caaatcctaa
     9661 tgccaaagca aagcaactta agagaataag tgagtattca agacgttttt gtttatacat
     9721 caagtagatc gtatagaaaa aggtccctat agcgataaaa acagcgatta tcatcgtcaa
     9781 tacttgcggc actgattgtg aataaataaa actaatcaat ggtccagttg ttcctagata
     9841 aactttcggt ggcagtccag catagttctg ataaggagat gagactgtaa gggtcaattt
     9901 ttttccgata taatttgctg gtaaatctat cacagccaat gagagtccgg gattgctttc
     9961 ttcctgataa tctttccgtt ggtataaaat cttttcgcct agatatacag taatccattg
    10021 atggtttcct ttgattagta aatttgcttc gtccaacggt ttttcaagtg tcgtttccat
    10081 aatgaatgtt tcgtctttat ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt
    10141 aaaatcagga tcatcttttg tatagaaaac ccacgaatct tttatttctt tcatttgatt
    10201 gctgtccgca ggagaaaaaa taaaataaaa gaaataagca attcctgtta aaaataacaa
    10261 tgataatata atgattatgc tccactttat acgtaaataa aaagattctt ttttattata
    10321 tatgttatta aaaaaagac
//
LOCUS       DACEMO010000091        10310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEMO010000091 DACEMO010000000
VERSION     DACEMO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.093"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..202
                     /locus_tag="IW728_001706"
     CDS             <1..202
                     /locus_tag="IW728_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAQ1025066.1"
                     /translation="GKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELV
                     NIMAKHDRELAKREAAMKNAVK"
     gene            282..1295
                     /locus_tag="IW728_001707"
     CDS             282..1295
                     /locus_tag="IW728_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025067.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            1332..2276
                     /locus_tag="IW728_001708"
     CDS             1332..2276
                     /locus_tag="IW728_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAQ1025068.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWKNGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            2344..3657
                     /locus_tag="IW728_001709"
     CDS             2344..3657
                     /locus_tag="IW728_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ1025069.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKTSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVISASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            3741..4643
                     /locus_tag="IW728_001710"
     CDS             3741..4643
                     /locus_tag="IW728_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAQ1025070.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSVSFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            4648..6300
                     /locus_tag="IW728_001711"
     CDS             4648..6300
                     /locus_tag="IW728_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1025071.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEKVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKADSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            6324..7190
                     /locus_tag="IW728_001712"
     CDS             6324..7190
                     /locus_tag="IW728_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1025072.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(7232..7423)
                     /locus_tag="IW728_001713"
     CDS             complement(7232..7423)
                     /locus_tag="IW728_001713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025073.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCGKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            7453..7992
                     /locus_tag="IW728_001714"
     CDS             7453..7992
                     /locus_tag="IW728_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025074.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGADVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            8120..8473
                     /locus_tag="IW728_001715"
     CDS             8120..8473
                     /locus_tag="IW728_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025075.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            8940..9416
                     /locus_tag="IW728_001716"
                     /pseudo
     CDS             8940..9416
                     /locus_tag="IW728_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            9416..9610
                     /locus_tag="IW728_001717"
     CDS             9416..9610
                     /locus_tag="IW728_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025076.1"
                     /translation="MAETRPKGYPKLKEYTPNRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            9616..9901
                     /locus_tag="IW728_001718"
                     /pseudo
     CDS             9616..9901
                     /locus_tag="IW728_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            9915..10281
                     /locus_tag="IW728_001719"
                     /pseudo
     CDS             9915..10281
                     /locus_tag="IW728_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
BASE COUNT         2928 a         2087 c         2626 g         2669 t
ORIGIN      
        1 cggcaagatg ctcgtttgtg taaatccaca gtggttccgt ccgactgacg ttgacaacct
       61 gtggggcgac ccgactaagg caaagaccgt tctcggttgg aatccgcaga agacctctta
      121 cgaggaactg gttaacatca tggcaaagca tgaccgtgaa ttggcaaagc gcgaagctgc
      181 aatgaagaac gcagtcaagt aagctgacat tgaataaaca tatctgtttc tatctttcca
      241 gtcacggctt cggacatatc gcgcggaatc tgcccattat tatggcaaca ctctgccgat
      301 gcgaagcgaa gattacactg gtttgcggcg caaatcatct ggattttgca aagcgcaatt
      361 tggagcagga actgcccaaa gagctgatag accgcgtgcg ctatcgtcca ataaacacag
      421 atatcggtct gattaaccgt gagggcaccc tactggtgga cacgggtaag ctgaccgaga
      481 tctgtgaata ttttttggca aaggttcctt ctttggcaaa gacagaagcc gaatggctca
      541 agcgagaagc tgtcagcgca gtgctttgcg atatgccaat ctgggcaatg gatgcctgcg
      601 atatggcagg agtacctttc ctgtatgtag gaaacttcac ttggacagag ctgtatcgcg
      661 aatttttacc cgaacatatt tggaaagaat atgccaagcg ttacggtaaa attcagcaca
      721 ccatgcttta tgcgctaaat aacgaagaaa tgttggaatt tctccaaaac agcgaaaaaa
      781 caccgacttc cgttgtggcg cgtccgattc ataacgacga agtgcaacgc attaaatcac
      841 agcatgaaaa accgcctgta tttgtcgcac tcggtatgtc cgcgcggttc aaacagccaa
      901 ttgatgtcag tcacgcgccg tatgatttca ttacaacgct aggtgttccg cttgtgggag
      961 aaaacgtaac cgttatttct aacagcaccc ccaatacaca ggactatata gcggcggcag
     1021 attacgttat tacaaaggct ggatggggaa ctgttgcgga gtgtattctc gcacagcgtc
     1081 cgatggcact gttcgcccgc gatacagttt tggaagatag aacaactatt tgtaaattgc
     1141 aaaaactggg tctggcaatt tccgtcacac cggaaaacct ccaccgcgta gagcgggtga
     1201 ttgaggacct caaacagcta gacacctcaa attttgatac attttatgat gctgcgagcg
     1261 aaatcgcaga caaattgatt tctctcatgc aatgagagat gctctaaatt tagaaaggag
     1321 caagcattat tatgatggaa aaagacgcaa aaatctacgt agccggtcat cgtggcatgg
     1381 ctggttccgc aatcgtgcgc gagctgaacc gtcaggacta caacaatatt atcacccgca
     1441 cccataagga gcttgacctg tgccgtcagg acgctgttga agcatttttc gcacaggaaa
     1501 agccggatta tgtatttctc gctgctgcaa aggttggcgg tattatcgca aaccagaacg
     1561 ctctggcaga cttcatgtat gaaaacatga ttctggaaat gaacgtcatc aactccgcat
     1621 ggaagaatgg ctgtaagaag ttacagttcc tcggttcttc ctgtatctat ccgcgtatgg
     1681 caccgcagcc gatgccggaa agctgtctgc tgacaagtga gctggaaaag accaacgagg
     1741 cttatgcact ggcaaagatt tctggcctga agtactgtga gtttctaaac aagcagtacg
     1801 gtactgacta tatctctgtt atgcctacta acctgtatgg acctaacgac aactatcatc
     1861 ctactcacag tcatgtgctc cccgctttga ttcgtcgctt ccatgaggct aaggaagcag
     1921 gtctgcctac tgttacctgc tggggtgatg gttctcctct gcgcgaattc ctatatgttg
     1981 acgatctggc aaacctgtgt gtgttcctga tgaataacta tagcggtgac gagactgtta
     2041 atgcaggtac tggcaaggaa ctgtctatca aggaactgac cgagatggta gcaaaggtta
     2101 ttggttacga aggcgagatt ctgtgggata ccagcaagcc gaacggtaca cctcgtaagc
     2161 tgctggatgt ttccaaggca accaagctgg gatggacata taagactgag ttggaagatg
     2221 gcatccgtct gtcctacgag gacttcctga ataacccgat gcgtgctgaa cgctaattta
     2281 ccgaggttgc tgtgattttt gaggattgca gcagcctctt tcttataaaa aggagagatc
     2341 attatgaata ttgttttact gtctggcggt tctggtaagc gcctttggcc gctgtctaat
     2401 gacatccgtt ccaagcagtt cattaagatt ttcaaaactg aagatggata tgaatccatg
     2461 gttcagcgtg tctaccgcca gattaagaag gtagataacg acgcaactgt tacgattgca
     2521 acctctaaga cacaggtttc tgctatccat aaccaacttg gtgaggatgt gggtgtatct
     2581 gtagagcctt gtcgccgcga taccttcccg gcaatcgcat tggctaccgc ttatctggta
     2641 gatgtgatgg gcattgatcc agaagagtcc gtagttgttt gcccggttga cccttacgta
     2701 gaagatgact attttgaggc cctgaaatct ctgtccgatc aggctgacaa aggcgaagct
     2761 aatctggtgc tcatgggtat tgaaccgact tatccttccg agaagtacgg ctacatcatt
     2821 cctgagagcg cagagcagac tgctttcgta aataccttca aggaaaaaac ttctgaggct
     2881 gtggcaaagg aatatatcgc acagggtgca ctgtggaacg gtggtgtgtt tgtctacaag
     2941 ctgaagtatg tcatggagcg tgcacatcag ctgattgact ttgtagatta tcaggatctg
     3001 ttcaacaaat acgatactct gactaagatt tccttcgact atgccgttgt tgagcatgag
     3061 gacaagattc aggtacagcg ttttgctggt atgtggaagg atctggggac ttggaatact
     3121 ctgaccgaag gtatggagga atccattatc ggtaaaggtg ttatgaatga gaattgcgat
     3181 ggcgtacaca tcctgaacga actggatgtt cctgtgttgg ctatgggtct gcatgatgtg
     3241 gtcatctctg catctcggga aggtattctg gtttccgata aggaacagtc cagctatatt
     3301 aagcctttcg ttgacaagtt cgagcagcag atcatgttcg cagagaagtc ttggggtagt
     3361 tataaggtta tggatgtaga agcagagagc atgaccatta aggttactct gaatgctagt
     3421 cactccatga attatcacag ccacaagaac cgtgacgagg tgtgggtggt tctggccggt
     3481 actggtaaga ccatcgtgga tggatatgag caggttgtta aggctggcga tgtgatcacc
     3541 atgagtgctg gctgtcggca taccattatg gcagatactg agctgaagct gattgaagta
     3601 cagcttggca aggatatcaa tgttaaagat aaacagaaat ttgagttaga gtattaaaaa
     3661 acggaggagg gctcgttata cagaaaccgt ttttattgaa gccttccgca aaggattatc
     3721 tgtggggggg aaccgcctga atgatgaatt tgcaaaagga attgagatgt ctcctcttgc
     3781 ggaaacttgg aatgctctac gcacccggat ggaccgagct ttgttgtgtc tggtcagagt
     3841 atcaggggat gaccttgacg gaggtgttgc aagaacaccc agagtttctg ggaacgcatc
     3901 cgcagaccaa aggagaattg ccgatcctga tcaaattcat tgatgcgaaa aaggatttgt
     3961 ctgttcaggt acatcccaca gatgaatatg caattgaaaa agaaaacggg cagcttggaa
     4021 agacagagat gtggtatgta ctggatgcca gcttaatcta tgggctgcag aaaataacag
     4081 aagaagctgt gatccgtgaa gcgattgctc agggaactgt tgagcgttat cttcagaagg
     4141 ttccggtcaa aaagaatgat gtgttcttta ttgaggcggg aacgattcat gcgattggtg
     4201 cgggtgcact aattgcagaa attcaggaaa attccaatct gacttatcgt ctctacgatt
     4261 atgaccgtgt tgacaaaaat ggtaaaaaga gagaacttca tgtagacaaa gcgctagaag
     4321 tagcgaactt gaaagggttg tcagaaccga aacagccgct tcgtgttcta aaatatcgtg
     4381 ctggtgtagc tgcggaattg ctttgcagat gcaagcattt cgaggtctct cgtatgattg
     4441 tcaacactga gagaagacag acggtacact atcgtgcgga ttcagtcagc ttccgtgtgt
     4501 tgctgtgtgt caatggctgt ggtaccgttc gatttgataa tgaatcgttg gacatctata
     4561 agggggactg cctttttgtg ccggccgatt ctactgaaat cagcattcac gggtcgattc
     4621 agtttctgga tgtgagagga taataatatg aattataaag tagaatatga gcgttggctg
     4681 aatcatgcca atgcagatat tatagaagaa ctgcgttcct attctgagaa ggacattgag
     4741 gactctttct ataaaaattt gtctttcggt actggtggtc tgcgtggtac catcggtgca
     4801 ggcactaatc gcatgaacgt acacaccgta ggtaaggcaa gccaaggtct ggcagactat
     4861 ctgaataaga actatgaagc cgcttccgtg gtggttggct atgattcccg tatcaaatct
     4921 gatgtgtttg caaagactgc cgcagacatc tttgctgcaa atggtattca ggtgcacttg
     4981 tggccagaac tgcttcctgt tcctacagta tcttttgcga ctcgttatct gaaggcagat
     5041 gccggtgtta tgattacggc atctcataac ccttctaaat ataacggcta caaggtatac
     5101 ggtgcagacg gctgtcagat tactaccgag gctgctactg caattctggc agaaatcgaa
     5161 aagctggaca tctttgctga tgtaaagatg gatggcgatg cttctaagat tgcatccatt
     5221 cagtcggaag ttctgactgc tttcatcgaa gaagtgaagg ggcagtccgt cctgtttggc
     5281 gaaaaagtgg acaagaatgt tgctatcgtg tattctcctc tgaatggtac cggtctggtg
     5341 cctgttaccc gcaccctggc agagatggga tacagcaatg tgaccgttgt ggaagaacag
     5401 cgtctgccgg acggtaattt cccgacttgt ccttatccga accctgaaat taaggaagct
     5461 atgtctctgg gtatggagta tgccaagaag tacaatgcgg atctgctgtt ggccactgac
     5521 cctgactgcg accgtgttgg tatcgcagtt aagagcgcac atggtgacta tgttctgttg
     5581 tctggtaatg agactggtct gctacttctg gactacatct gttctcagag aattaagcat
     5641 ggaaaaatgc ctgctgaccc tgtgatggtc aagaccatcg tcaccatgga tttgggtgaa
     5701 cgtatcgcat ccaactatgg tgttcgtacc atcgatgtgc tgaccggctt taagtttatc
     5761 ggtgagcaga ttggtatgtt ggaaaaggct ggtaaggctg acagctatat cttcggcttt
     5821 gaggaatctt acggctatct gtccggtagc tatgtccgtg acaaggacgg tgttaacgct
     5881 gcttatctaa tctgtgaaat gttctcttac tacaagactc agggtatcag cctgctggat
     5941 aagctgaatg agatttatac aaagtatggc tattgtttga acaccctgca ttccttcgag
     6001 tttgacggtg cggcaggttt taccaagatg caggaaatta tggttgaatt ccacaagggt
     6061 ctcgatataa ttggtggtaa gaaggttatt gaaactttag actacagtaa gggcctgaat
     6121 ggtctgccga agtctgatgt tttgaagttt atgctggaag ataactgctc tgttgtggtt
     6181 cgtccttctg gtactgagcc gaagctgaag acctatatca gcgtcagcgc agagaacaag
     6241 gaagccgccg aagctatgga agctattatg gctgctgaac tggcggaaat gttcgagtaa
     6301 gaaaagaaag gagaaatctt gtaatggagg aactgttttc taacgctttg aaatcggtga
     6361 agtcaaatcg tgtgctgtac acggcatcgt tctttgcaag atcctctctt ttgtatttgc
     6421 aggaaattgg acagctgact gctctgaagg cacatacatc taggcgtaca ggattgtcat
     6481 cttacctttg tttcctggta gaagttggtt caggcacatt ggagtataag gggcagaaat
     6541 atacgcttca agtaggagat atggtgttta ttgactgttt gaagccgtat gtccatgagt
     6601 catcagaaga tgatttgtgg actttgcggt ggtgccattt taatggcatt ggtatgacgg
     6661 aagtctacaa aaaatacctt gaacgaggtg gtcaaccggt ctttaaaaca tcaaaaacag
     6721 ataagtatgc tgacttgctg aggagccttc atatggtggc agagggacag gagtatgtcc
     6781 gggatatgaa aatcaatgtt ctactcagtg aactgctgat gcatttgatg gaagattctt
     6841 ggaatcctga caaagtgccg aatttctcta caaaaaaaat ggaattgcag aacataaaac
     6901 aatatctgga tgaccattac acggagaaga ttacgctgga tcagttgagt gcagagtttt
     6961 ttatcaataa acagtactta gctaagattt ttcgtgaaac ttacggtaca actgttgtcg
     7021 gttatctgaa tcagaaacga atcacacagg ccaagcactt gctacgcttt acaaatctaa
     7081 gtatggaaga gattgcaaca aaaattggca taacagatgc aaactatttt tccagaactt
     7141 ttttcaaggt ggaaggtgtt cgtcccagcg aattccgcaa gatgtggtaa taaaaagccg
     7201 gcggtgttga gccgccggtc agtacctgtc gttaacccgc attcaaagct ttaatctcag
     7261 ctttgatttc agcacgaaca cgatagtaat cttcagtttc gatttcggta tccagccgag
     7321 ttgctgtctt tccgcagtca ggacaacgct ctggctgttc gtcggcaggg aagctgtagt
     7381 ggcaggaatc acaggtgtaa atgtgatcgt cacgtataat catcacctac caatccagtg
     7441 cttagatttt tcatgcaatt actattatat cgaattggtg attccacaaa aatccgcttc
     7501 ttgataaatg tacgattcag agtgatatat agtactgtac tgccaaaggg aataccacaa
     7561 cggcagaaat ggatgattca catggaagaa attatgcaga tgattttagg gaacgcaaaa
     7621 ttccagctcg gacaggtggt ttcgacccct gctgtattgg aaactttcaa ccccctgcag
     7681 accgcaaacg gcattcccga agccttgagc aacctttggt ttagacagga aattagtatt
     7741 gcggagatcg aggacgcagt tctggatgcc aagaacggca aggagctagc aaacagcctc
     7801 aaccgcatca agcttttccg caagttcacc ctcgaccgag agctggacgg catggtcaga
     7861 ctgaagggcg cggacgtctg gaagaacatc agctacctca aggtctacac cgccacgccg
     7921 cctaagaaac cgaccccccc aaggttccag tcgaacggct ggagcctttt cttctctcat
     7981 aaatcggcat aatgtataca aatacagctc caaatgattg tgtagtatat gcctgtggat
     8041 tgacttgata tagtcgagag actatggcaa tatacacaca accaaagggg aaaatcccaa
     8101 aaacaaggag gaaacgctaa tggcaatgag cgaaaaggca aagaaatatt tgaaggagat
     8161 caagggtgca aagaccatcc cggagctgaa ggatgtggag atcgcaatca agcgagacgg
     8221 tatccttgct tgggcagagt tcaccaagtt gaacgaggcg gtggaagaga agaaggtcgc
     8281 ccttcgcaag aaaaagcagg agaccagcct tcaggagatc cttttctggg cctacaaaaa
     8341 ggaatcagac aagctgctga agatgatgga cgagggagca gacaaggatg ccatccagat
     8401 gcaggtccag cgttacgaca gcatcggcca gatcattgca gaggcagacc tcgaagatga
     8461 gtatgaggtt tgagaataca gcctgaacca ctaagaacca aataccgaaa gggacttctg
     8521 aaacagacag aggtcccttt cttttgctca ctttggcggt caattatgaa ccaaaaacca
     8581 gtcgaatgat tgtgtacatt atggctttca aacgacttga taataatcac ttttagagtg
     8641 atatatgtac tacaaaaaac aaaagactgg agaaaacaac agatgagcga atacagaatc
     8701 atttatatca gcgacgagga cggcggcgag gtggaaggat gtggctacac ggctacgatg
     8761 gttatgatgc ggtcagaaac ttcttggaat ttgcctgcgg catcttgaaa atcctgcggg
     8821 tgcgattcat cagaagagtg gactgaaata caagttagca gatagagaag agagtcaggt
     8881 ttcgacttgg ctctgttctc tgtctactgg cagaaataca ttgaagggag gtgacgactg
     8941 tggcaaccag aggaagaaaa tcaaccccga cagccatgaa gaagctggag ggaaatccgg
     9001 gcaatcgccc actcaatgca aaagagtcaa cgccggaaaa gaaggcaccg acctgtccga
     9061 agtggctgga accagaggca aagaaggaat ggaagcgtct ggcaaagcag atggaggcca
     9121 tcggaatctt gacagatgtg gatatggctg cctttgccgg ttagtgccaa gcctacgccc
     9181 gctggaagga agccgaggaa ttcattaccc agcacggtat catcgtgaaa accctatccg
     9241 gctattggca gcaggtacca caggtgttta ttgcccagac ctatctgaaa attatgaacc
     9301 gattttccga gcagttcggt ctgacgcctt cgtccagaag ccgtatcatt gccggtgatg
     9361 gcggtggtgt cggagcagaa gatgcgatgg aggctctgtt gggaggtggt agctgatggc
     9421 agaaaccaga ccaaagggct atcccaaatt aaaggaatac acaccaaacc gattcatgct
     9481 ttcggaatgt cactacgaca aagccagagc ggatcgggcg gtgaacttta tcggacagct
     9541 ccgacacacc aaaggcaaat gggcaggcaa ccgcttctgg ctcttgcctt ggcaggaaca
     9601 gatcatctag tgtaatctcg cttaactgta ttaacggacg tagcgacttt attaagagtg
     9661 ttcatggtag gagcattagt tgcaacaatg attgatatta aatttttatg gtaccaacga
     9721 ttggcgatgt gacattgaat atgcaaaact atatcgatat gatattatct aaaatcttac
     9781 tcgctgggat cgtcgaggtg tttattggtt gttaggaaag aaaaatatga cccaactcga
     9841 gcgattttta tcgtattact tgtgtgtggc tttatctgca ttgtgtgtga tttcgaaata
     9901 agggagagcc aaaaatgagt agaaaattga tattgattag tcacggaaaa ttttgtgagg
     9961 aattgaaaaa aagtacaaaa atgattatgg gacctcaaga gaatatctac acgttacctt
    10021 tattaccaga agatggcact gaaacataca gacaaaagct cttactgtca tagattcgtt
    10081 aaatgatttt gtggtactat gcgatctttt aggcggtaca cctactaata tcatttctaa
    10141 actgataatg gaaggacaac agattaaact ttatgtcgga ttaatcttcc tatggtcatt
    10201 gaatttatca actcgcaagt gattggttca gagcccgagt ttgttcgtgc aggatgtgaa
    10261 agcattgtat ctgtcaacta attaattagt ggaatagtag aagatgaaga
//
LOCUS       DACEMO010000092        10219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEMO010000092 DACEMO010000000
VERSION     DACEMO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.094"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            73..2691
                     /locus_tag="IW728_001720"
     CDS             73..2691
                     /locus_tag="IW728_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1025077.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEKDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTDGLLATANNGYLFLDEVHRLSKENQEKLFLFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKGENYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADVLQKDIIVSREALSLMLSHPFSGNIGKLKNIIKISCADVYSRTQEEPLTIGKN
                     EILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNEN
                     FSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGPVAEIISR
                     SYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSEKDENFLRLLIVVTLSD
                     YVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMPVESNVSEIVESTRNFV
                     KRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNAPFK
                     QIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEVFTK
                     DYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANLEHILSP
                     EMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHFTLNGRV
                     KLNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSRSIFQSMEMKYNFKVNA
                     YELSLLYELLGPYIQK"
     gene            2768..3256
                     /locus_tag="IW728_001721"
     CDS             2768..3256
                     /locus_tag="IW728_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1025078.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            3268..3762
                     /locus_tag="IW728_001722"
     CDS             3268..3762
                     /locus_tag="IW728_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1025079.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3783..4592
                     /locus_tag="IW728_001723"
     CDS             3783..4592
                     /locus_tag="IW728_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1025080.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4589..5410
                     /locus_tag="IW728_001724"
     CDS             4589..5410
                     /locus_tag="IW728_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1025081.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5461..7560
                     /locus_tag="IW728_001725"
     CDS             5461..7560
                     /locus_tag="IW728_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1025082.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7557..8675
                     /locus_tag="IW728_001726"
     CDS             7557..8675
                     /locus_tag="IW728_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1025083.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8714..9592
                     /locus_tag="IW728_001727"
     CDS             8714..9592
                     /locus_tag="IW728_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1025084.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9783..9935
                     /locus_tag="IW728_001728"
     CDS             9783..9935
                     /locus_tag="IW728_001728"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025085.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         3131 a         1677 c         2279 g         3132 t
ORIGIN      
        1 tacaaatata gaatagacat attgcagccg atagtttatt atgcttttag acacattaaa
       61 ctggaggaaa aaatgaaaaa gagcgctaaa gatcgagttt ttgatgagct gatacgacta
      121 acagggaata tgtcttctgt cactgccgag aaaatttcga agaatctagg tatctctcgt
      181 cagaatgcca gccattattt gacgagatta gtggaagata aaaaggtcga gaaattaccg
      241 ggaaagcctg ttttgtggaa gcctttagat gaatatgcgg ttgtagataa tacggagcaa
      301 ataaacgaag cctttcattc tgtagttggt catgacggaa gtctgagaga ggtcattcaa
      361 aaatgtatag ccgctgtcaa atatccgcct aacggactca gtgtattaat aaatggagcc
      421 acgggtgttg gaaaaagttt tttagccaca aaaatttttg aatacgctat ccatgagcaa
      481 attattgaaa aagatgcacc atttgctatc ttaaactgtg ccgattatgc ggacaatccc
      541 gaactgcttt cggcaacact gtttggatac aagaaggggg cctttacggg ggctgaaaag
      601 gataccgatg gtttattggc aacggccaac aacggctatc tttttctcga tgaggtccat
      661 cgcctatcga aagaaaatca agaaaagctc tttctattta ttgatactgg aaactatcgt
      721 ccagtcggtg aaaatgtaaa ttggcattct gctaaggttc gttttatttt cgccacgacg
      781 gaaaaaggtg aaaattattt attagacacc tttgatcggc gtattcaaat tagtgttatg
      841 ttgccaactt ttgaagatcg ccccattcga gaacgattag agctgattca attatttttt
      901 caaaatgaag cggatgtttt acaaaaagat attattgttt caagagaagc cttgtcttta
      961 atgttatctc atccctttag tgggaatatt ggtaagctga agaatatcat taaaatcagt
     1021 tgtgcggatg tgtatagtcg aacacaagaa gaaccattaa cgattgggaa aaatgagatc
     1081 ttgattcagt taaatatgct ggaatccgaa gtagaaagct tgccgattgc tagtttattg
     1141 ataaagaaaa atcaaaagat aaaaacggat cgaatcaaac cgtttgatta tgattcgatt
     1201 ttggaaaaaa ttactcagct gataaatgaa ggtaatttca atgaaaattt ttcttcgata
     1261 aagaatgaag tacagcattt ggtgcatcac attgataagg attctttttt atcaatgagt
     1321 agaaaattga tgattcagat gtttagaaag gtctgggtat ccgttttagg aaaaacgtac
     1381 ggattgacga cgagtggacc tgttgcagaa attattagca gatcctatgt catttcccca
     1441 agctataaga cagacttaac cgggataaat cgtacattag ctgcaaatct acctcggacg
     1501 acttacttat gtaatcagtt cttggagcat ttaccaccgc tctcagaaaa ggacgagaac
     1561 tttctaagac tgcttattgt cgtgacttta tctgattatg tagatgaatc catcgagctg
     1621 aagggcctgt tgcttgctca cggagatagt acggctagca gcattcaagc agtagtgaat
     1681 caattatgcg gcaattatgt ttttgaagcg ttggatatgc cagtagagag taatgtgtca
     1741 gagattgtcg aatcgacaag gaatttcgtg aaacggcaag tgaatacaga tagtctagtg
     1801 ctgatcgtag atatgggctc tttaaatcaa atttacacac agatcaagaa cgatttaaaa
     1861 ggggagttac ttcttctgaa taatttgacg acttccattg cacttgatat tggcttgaag
     1921 atgacgatga atgcgccatt taaacagatt gcggaggatg cagcagacaa gtattcaatc
     1981 aatgttcaat actttgaagg cttttctcaa agtagcaaca ttatcatttc ctgtatgtcg
     2041 ggtttgggga tttcggacaa attgaaagat gtttttcgtc atcatttatc ggatgaatcc
     2101 atcgaagttt tcacgaagga ctacagagaa ctacgtgaat taatcgacca aaatgatgaa
     2161 acttattttg acaagacaaa attggtcatc acgacttcgg atcttccacg ttccttttca
     2221 attcctaata ttaatgttta tgacatctta gacggtgatg gagcagccaa tttggagcat
     2281 attttgagtc ctgaaatgaa gccggcagaa tttaaccgct tgattcagga gctagtaaaa
     2341 ttcttttcgt tggaaggaat tgcggaccgt ttgaactttc tgaacccaac gatcgtgatg
     2401 agtgaagtgg aaacagtcat ttctaaatat gaaaattact atcatttcac tttaaatggc
     2461 cgtgtaaaac taaatctcta tatgcatacg gcattgatga tggaacgttt attcttggct
     2521 cggacagaaa aatctgaaga accgaattat gatttgaaac cagatgaaga tgaattttat
     2581 tatgtttcta gaagtatttt tcaatcaatg gagatgaaat ataattttaa agtcaatgct
     2641 tatgagcttt cgctgctcta tgaattatta ggaccgtata ttcaaaaata aagtgtctaa
     2701 aaaagtgtct aatctgtttt ttttaggtta gacacttttt ttaatgtgaa ttaacgtgat
     2761 taaaccaatg tttttaaaag ttggcacagg aattgcttat ataaaagtga acagaaagaa
     2821 aggaattaac catatgaaga aaattctaat agcgactcac ggtcacttag cttcaggctt
     2881 tcttagcagt attcaattac tgacggggaa ggttcaagag attacggtga ttaatgctta
     2941 tgtggatgag tgtgattttg aacaggagtt gaatgtattt gtaaaccagg tgaacgagca
     3001 ggatcagatt tttgttttta ccgatttatt tggcggaagt gtcaatcaga agattaccaa
     3061 aacctttttg gagaaagaga ttgctgtaac tattttgtct ggctttaatc tgccaattct
     3121 gctcgaaata gtattggcaa ccaaggaatt aacaaaggag gatattcagg aactggtaaa
     3181 taaatgccaa caggaattaa agatgaccat tcttgatgag gtacatgtag taaatgaaga
     3241 ggaagatttt ttttaggagg aagaaaaatg attaaagcag tgcgaattga tgaacgatta
     3301 attcatggac aagtagcgat gacttggaca aagagtctaa atttaaccgg gttagtagta
     3361 gcaagcgatg aggcggctac caacgatatt caaaagatga cattgaagat ggctgcacca
     3421 tcgaatgtca aagtcattat taaaacagtt gatgacgcga ttcatttgtt aaaagatccg
     3481 cgggcgaagg acatgcgctt gatggtttta gtccctaatg tcaaagatgc ggtaaaagtt
     3541 gcgaaagctt tcccaacgga aatcgaatta atgaacgtgg ggaatgctgg gaaaatgaca
     3601 ccgccatcgg aagataagaa aaccttatca aaggaagtga tgttaacgac gtcagaaatt
     3661 caagacttaa aagaattagt tgaactttat ccagataccg tttttcagcc tacaccggcg
     3721 atggaaaaac gttcagctat ctctattttg agtgattttt agaaaaagga aggagaaaaa
     3781 ttatgtatga tgcatttgta gtcgctttgg ccgtttttat tggagttgct ggacatgaaa
     3841 tgttcgggat gtccatgctg agtcgtccga ttgttgttgc tccgttagca ggagcgttga
     3901 tgggggattt gcagacgggg ttgattgtcg gggcgtcact agaagccatc tttatgggtg
     3961 tcgtcaatat cggaatctct agtacggcgg agccttcttt ggctgctggg ctggctacag
     4021 cgttcgcaat caaaatggga ggaaacgtgg atgcgattat tcccattgtc tttccactag
     4081 cagtattagg attacagtta atgaatatga ttttttcttt tgtatgtgga ccgatggctg
     4141 ctaaattttt caaatttgca aaatctggtg atcaaaaagg aatcatcaat ctgcacttct
     4201 ttatgtggtt tgtccactat gcgctttatg ctcttattcc attctttgcc gttttatttg
     4261 gagcagatgt tgtcaaaagc gtcttggata atattccaga ggtcatcatg aacggattaa
     4321 cagtcgctgg aaatctgctg ccggcagttg ggatggcgat gctgttacgg atgctttgga
     4381 gtaaaaatat tgctgtgtgg tttttctttg gggccgttgt tatggcgtat ctcgagttac
     4441 cattgattgc aattgccgca gttggaacga ttattgcaat tgttatagcc caaagagatc
     4501 tacaactaaa aaatatatct gttcatgcag ttcaaagcga tgctgctgta aatgcaacgc
     4561 ttgaccaaga agaggaggat ttctttcaat gaaattagct gatggaattt ctaaagatga
     4621 gaaaaaattg gttcggaaga tgttttggcg ctcgatgact atgtatgctt cggtgaatcc
     4681 ggtgacgatg ggcggcggtg gtttcgccta ttctatgatg cccttcatta atcatttcta
     4741 caaaagtgaa gaagatcgag ctgcggcgct ggaacgtcat acacagtatt tttcgacgac
     4801 cattccttgc gcgtcctttg tcatggggat tgcggcatcg atggagaagg aaaattctga
     4861 aaaagctggt tttgacgtgg aaagtatcaa cgcgatcaaa acgagtttga tgggaccatt
     4921 ggctggaatt ggcgattcat tgttctgggg agtttggcgt gtcgttaccg cgggactagc
     4981 gatcaactta gccaatgccg gaaatatttt ggcaccaatt atctttttag ttatgttcaa
     5041 tatacctaat tacttgatgc gatactatgg cggcgtttta ggctattcat taggctctaa
     5101 atatattgaa aagctgtatg cgaacgggtt aattgaaatt ctgacaaaag ctgctagtat
     5161 cttgggtctg atcatggtag gtgcaatgac gatcagtatg gtcaaattca ataccgtttt
     5221 gagcttttca atgaagggcg aagaactaat gaatgtccaa gaaatcctcg atagtatttt
     5281 taaaggaatt gtaccgttat tggtaacatt gagctgtttc aaattactag ataaaaaagt
     5341 cggcgtcatc aaaattattt ttggaatggt cgtcttggga attttattgt cattacttgg
     5401 aatcgcatag taggaagctg cagctactag gctgcagttt ttttagcaag gagggaaaag
     5461 atgactatta atgaagtaat ttattcaccc aaacacccat tttttaatgc tgagttaaac
     5521 gacggaaatg tgactacttc aaacgaatac agctactatc ggaatattcc tgatgtaaag
     5581 ggattgacct tcgatgtaag taatcaaaag acattagcaa accttgatgt ttgcgggacg
     5641 attcaaacgt tgacctttta tcgagaaaat ttactaactg aggaaaaacc aggtgtttgg
     5701 gtcaataaac agctctctca aaccaattcg ttatccttaa aggttgaagt aaacgggatt
     5761 gtctaccatt tgtcagaatc ggatcatcgt attgaagtcg atgtgattca agactgtttg
     5821 cctagatata ttcatcatta ttcgacgttt aaggtcattg tagtatcctt tgctcccata
     5881 ctggctaata agcgtttaag tatgcttgtt caacaggttt acattgtgaa tgaaacacac
     5941 gaaagccttc aagtttcagt gaaagatgtg ccttcgtatc aagagaagta ttcggatcag
     6001 caaaatgtgt tgatttctca aaaaggaaat aaggacacga tcgcacaaca ggaagtggca
     6061 agcttcgcca ttgcttttgt ggacccaaat gcttttgaag aagtaatgac ttttcaggaa
     6121 tctgatatcg aaaaatggct gaaggaaaca ttgaactatt ttgctcagct atttgggcag
     6181 ctgtcaatgc caaacgatag agttgtccat ctctatcgtc gtgcacttta tcaaagcttt
     6241 tctagtttcg gtatgaatcg acagggacag gtcgtcggca gcaattgggg cagctatcct
     6301 gctactaatc gaatttggaa taaggatatg tattacgcat cattgccatt caccatgttt
     6361 gaaccggagt tatgtcaaaa aacggttcta tggtttgatc aatatggcgt taagtttcct
     6421 ggttcaaaat ttacaggagg tatcaatcat tctctgagta attcgttatc cagtgcgtta
     6481 ctggctagtc tttattttga acatactggg gatacaaaat tctttgagcg gtgtccgcag
     6541 gttctactga atgcttcaag aattattgag gacatattag ctcaacgtcg gtcaggggaa
     6601 ccaatgcttt ttccatctgt ttggttatca gacgcctttg cacttggaaa gtatcatacc
     6661 ggcagcaatc tttgtctttg gaaggcctgt attggacttg cagaaatttt tgaagcccta
     6721 gaacaactga gtcttagcaa gcgatacaaa aacattgcct gtaaactaaa ggaatcaatt
     6781 tgtaaacaaa tgactatcga tggtcctttt ggtgagcaat ttttggaggg aatcggtgat
     6841 gaggagaaaa caacctattc agtgaagcac tatcaaaaac cgattcttga gcaagggtta
     6901 atctttttaa gtgatgtgat tgtggatgga aaaattaatt tattgatgca tgatggagaa
     6961 gaatcagaca ctacgttgat gcctttttat caatttttag ataataaaga tcatttatat
     7021 cagaacacca tggccttttc tgctagttct ttcaatccaa cctatagcga ggaaatcaaa
     7081 ggcatcactt ggggattaga atccggcgcc acttttcctg gctttatcac tgttttaatg
     7141 agcgagctgc acaaccctca agctttcgta acccgattag aagaactaat ttgtttggca
     7201 gatttagacg gttcgtggtg gtggtggccg tatcgcttag aggctcaaca aggagaagtc
     7261 gtgcgagact ttggctgcgg caaatgcggc tgggcatcgg gtctttttgt cagcctaatg
     7321 atcagtcaat atttcggatt gaaattcaaa aaaggagtat tgcaaattga tcctgttgat
     7381 aatttaacgt tttcttggaa gaatttgaag ttcggtcaag ccttcatttc gattgaatgt
     7441 acgcaatccg agttgagtat ttcaaatttg tcagaaaagc cattgaaaat aagtgatacg
     7501 aaaaaaattc ttcacgtaga aaagggaaaa acgattcaca ttcaaaggag aaaaagatga
     7561 gtatactaaa aaatattacc caatttaaaa aaatccagag tcgagcaatc actcccgaaa
     7621 atccaacagg cgagaaaggt aaagctgcga tgttgccaag cgagctagga ccaagccgca
     7681 aaggtcgtgg cgcaattccg ctgacttctg gtgaagaaac tgtgattgcg gatattactg
     7741 gaacgggaat aattcgtcat atgtggatga cgattcgtga tcgaacagaa tttggcagtt
     7801 ttgttttacg tgatttaatc ttaagaattt attgggatgg gtcggatagc cctgctgttg
     7861 aaagtccttt aggggatttc ttttgtaatg ggtttggaga gcgttgcgag gtgaactcgt
     7921 tgccgatagt ggttaatcct acagggggct ttaattccta tttcgaaatg cctttccaaa
     7981 catccgctaa aattacgatc gagaatttac atccgaagga tatgtcctca tttttttata
     8041 cgataaatta cgctttggtg gacgaattgg aagaaaatag cctatatttt cacgcaaatt
     8101 ggcatcggga acgcattacc aaattacagg aggattataa gttaattgat cgattaaagg
     8161 gtcaaggcta ttacgtgggt acttaccttg cgcttacctg cctagaacgt tattggtggg
     8221 gcgaaggcga gtttaaattc tacattgatg gggatagtga gtatccgacc gtaacctcaa
     8281 caggttcaga agattatttt ggcggggcgt gggcctttca tgagtacgat gagttcggac
     8341 gtccacatgc gaaaaactat tcaacagcct ttttagggta tccttaccaa tcaaatagag
     8401 atgccacaag agatcgtttt gaaacgggaa aattgaatgc cgttcatgcc tttggtgacg
     8461 acgcaatgcc acgacatggt ctttatcgtt ggcatcttca agaccccatc tgttttaccg
     8521 aagatttatc ggtcacttta caacaaatcg gcaacgatga tgttcgctta tttgagcggc
     8581 aagacgatgt tgctagtgtc gcttactggt attcaacaga agcttcagga aatactaaac
     8641 gtcatttcac tgatcgagaa cgcttgccgc gttaatgaag tgtttttcac atattcgaag
     8701 ggaggaacct atgatgatat atggggcaat tgaagctgga gggactaaat ttgtctgtgc
     8761 agtcggtact gaggaattaa cgattttgga aagagtcagt tttcccacaa caactccagt
     8821 tgaaacaatg aagcaggtca ttgatttttt taaacctttt aaggaacaat tggcaggcat
     8881 cggggtcggc tgctttggtc caattgatat tcaatgcaaa tcaacgagtt tcggtcatat
     8941 cacatcaaca ccaaaacttg cttggcaaaa tttcaatttt gtaggaacgc taaagcaata
     9001 tttcaatatt ccgattgaat ggacaacaga tgtcaatgcg gcttgctatg gtgaatatgt
     9061 tgctggttct ggcaaagggt tagcaggtgt tgcatacttc actgtgggaa caggtatcgg
     9121 aggtggtgcc ttgttcaatg ggactttcgt ggaagggttc agtcatccgg aaatggggca
     9181 tatgttgatc aagcgacatc caaaggacaa tttttcagga aactgtccct tccaccaaga
     9241 ttgtttagag gggatggcag caggaccagc gattgaaaaa cggttgggtg taaaaggcca
     9301 gaatttattg gctgacgatt cgttttggca gattgaagcc ttttaccttg cccaatgtgc
     9361 atacaataca acactgatgt tttcgccaga tagaattatt tttggcggtg gtgtaatgaa
     9421 acaagaacac atgaagaaaa aagttcagga taaatttgtt gagcttataa atggatatgt
     9481 tgaaatacca ccaattgata gctacattat tacgcccgaa ttaggtgata atgccggaat
     9541 aatcggcgga ttagcattgg ccagaaaagc ggtaagaaat aagcaaccat aattaatgaa
     9601 tgtttcattg atactcatgt caaaagtatt taaccgagta atcaaatgat caaatgaagt
     9661 tggataaagc gaaaatgaat tcaaataaaa aggctctaag tcaaatcgtg ttgttttcat
     9721 taaatcactg aaaggaaatg cttctgcgtt tccttttttc tttttgttgt gcgctgtata
     9781 ccttgaggat attcactggt ggcgtacact taaatagtaa tatattgtgt tactttatgt
     9841 ctgtggtaat agttatagtt gcttaccaat gtatgttcat ttttcgaaat gttgacttca
     9901 taatccttgg cttaatgttt gaattttttt ggtgatagat tttttcagcc gtataaaata
     9961 aatcgtaatt ctattataga gagttgtgat tataaaaaga tagcaagaaa aaaaaagaaa
    10021 gtttctatac attttaaata ttttattcct atttctattg ctaaaaaagt agaaattcta
    10081 tatttacctt taactctcgc ttctcttgca aatgtagtta gcattttaat cgataataaa
    10141 ctcaggtagt tatattacct tgaaatgatt gataattttg tcacttctat tttttttgca
    10201 ataaattttt tattttagt
//
LOCUS       DACEMO010000093        10183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEMO010000093 DACEMO010000000
VERSION     DACEMO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.095"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(9..530)
                     /locus_tag="IW728_001729"
     CDS             complement(9..530)
                     /locus_tag="IW728_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1025086.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="IW728_001730"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="IW728_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1025087.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="IW728_001731"
     CDS             4610..5497
                     /locus_tag="IW728_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1025088.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="IW728_001732"
     CDS             5494..6663
                     /locus_tag="IW728_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025089.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="IW728_001733"
     CDS             6687..8042
                     /locus_tag="IW728_001733"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1025090.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IW728_001734"
     CDS             complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IW728_001734"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1025091.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT         3170 a         1936 c         2233 g         2844 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccgat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacgg ttgattttta ccagcacttg gcggctgtct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagcatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 cttcaaagtt ttcgattacg ccgttgtgaa cgagcacgaa gcgtccactt tgggaagtat
     9661 gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccatcgtgta tgaccaatac
     9721 ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg
     9781 ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac
     9841 ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca
     9901 ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct
     9961 tccagctgca ggaaaatcct tttgttttat ctttgcagac attttttgtg gattttctgt
    10021 agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg
    10081 tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctttttg ttttttatct
    10141 ggaaacattt aaaatcataa ccttttgcga atgttcttgt caa
//
LOCUS       DACEMO010000094        10074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEMO010000094 DACEMO010000000
VERSION     DACEMO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.096"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IW728_001735"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IW728_001735"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1025092.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IW728_001736"
     CDS             complement(1174..1869)
                     /locus_tag="IW728_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1025093.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IW728_001737"
     CDS             complement(1882..2598)
                     /locus_tag="IW728_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1025094.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="IW728_001738"
     CDS             2928..3536
                     /locus_tag="IW728_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1025095.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="IW728_001739"
     CDS             3861..4184
                     /locus_tag="IW728_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1025096.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="IW728_001740"
     CDS             4366..5736
                     /locus_tag="IW728_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1025097.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="IW728_001741"
     CDS             complement(5866..7167)
                     /locus_tag="IW728_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1025098.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="IW728_001742"
     CDS             complement(7521..9512)
                     /locus_tag="IW728_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1025099.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="IW728_001743"
     CDS             complement(9656..9922)
                     /locus_tag="IW728_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1025100.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2951 a         2093 c         1826 g         3204 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagtcatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtg
//
LOCUS       DACEMO010000095        10045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEMO010000095 DACEMO010000000
VERSION     DACEMO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.097"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(509..733)
                     /locus_tag="IW728_001744"
     CDS             complement(509..733)
                     /locus_tag="IW728_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025101.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            933..3701
                     /locus_tag="IW728_001745"
     CDS             933..3701
                     /locus_tag="IW728_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1025102.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            3782..4201
                     /locus_tag="IW728_001746"
     CDS             3782..4201
                     /locus_tag="IW728_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1025103.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4213..4683
                     /locus_tag="IW728_001747"
     CDS             4213..4683
                     /locus_tag="IW728_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1025104.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4700..5479
                     /locus_tag="IW728_001748"
     CDS             4700..5479
                     /locus_tag="IW728_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1025105.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5466..6287
                     /locus_tag="IW728_001749"
     CDS             5466..6287
                     /locus_tag="IW728_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1025106.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6304..7353
                     /locus_tag="IW728_001750"
     CDS             6304..7353
                     /locus_tag="IW728_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1025107.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7366..8370
                     /locus_tag="IW728_001751"
     CDS             7366..8370
                     /locus_tag="IW728_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1025108.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9003..10022
                     /locus_tag="IW728_001752"
     CDS             9003..10022
                     /locus_tag="IW728_001752"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1025109.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFAVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3367 a         1656 c         2095 g         2927 t
ORIGIN      
        1 tgaaactcta tttacttctt ttttaatttg ttcttgcaat cttttttgct acgtaatttt
       61 tgtaaaaaag tgggttgaga gaaatctaat tgattttcaa agaaggagta tgaaaatgag
      121 aaaaatattt attcgtttat tgtgggtact atcttagagg aataaataat agtggagaaa
      181 actatggttt ggcgcagatt ctatcaggtt tgatggaacg aagacaacct cccgacatca
      241 agtcaaaagc tacttgagat tttgatctga aaataaaaaa agaaaacaag gtaggaacat
      301 caatgtttct accttgtttt cgtaatcaat gggaaaaaac taagagtggt gtacgtaatc
      361 aataaaaagg atggtgacaa cagtacctct ttactctctg aaagcatcat aactcgtttt
      421 tcctcattat gccaataaat tgttgttata ccacatataa ggatttgtaa tagctaaaaa
      481 cagacaggaa agttgaaatc tgtgaatctt attttgtatg gaaggattta tagaacgcaa
      541 aaatcaagat aaagatgaat aaaaatcctg tttcggctat atattgcgta tctacgccaa
      601 taaaacgagt ttgttctgtc atagccatat aaagcaaagg caaaaataca actacagcga
      661 gataagatcc gatgatttct cgaaaagttt tccaggaagt atgtttcctg tttttatatt
      721 tatccgattt cattgaatag tcatagcctc cttattcttt catatgttga aattccagta
      781 taaaaactgg aaggaactgt acgtttttta gaataaaaat tataagggat aagtaggctg
      841 ctgtctataa taagatatat ttttcaattc tttattaaag attttattga caaaatgaat
      901 ccgttttcta tgatggagga ggggtgaata agttgttgaa gaaggaaatt ttatgttact
      961 tagagaacca gacagccttt ttcgatttag aacatatgaa cgaggtcttt acagcacaaa
     1021 ggttagctga tcattttggg gtaaaaagaa acacagtcag tcattatttg aaccagttaa
     1081 atgatgagca actactagtc aaaatcaatt ctcgaccagt gatttatttc cacaaagaag
     1141 cttttgaaaa acaatttttt aagttacgca cgaattttta tttttctatc gatgatctca
     1201 aagaggaaca gccttttttc gcgcaaccaa aagacttatt ctcttttatg atcggatacg
     1261 acgccagttt gaaagaaagt atcgaacaaa tcaaaacagc tctttactat ccagatggcg
     1321 gattaccact tttgattaca ggcgaaagtg gcacaggtaa aagttattta gttaatttgg
     1381 tctatcagta ttgtctgctt catgatctgc tagaagattc tgcaccattc atcactttta
     1441 attgtgcaca atatgcagat aatcctgaac ttttgactag caatttgttt ggacatgtta
     1501 agggagcgta cactggtgca gaagaaaata aaaaaggggc ttttgaagca gcagatggtg
     1561 ggatcttgtt tttagacgag gtacatcgct taacaccaga aggtcaggaa aaactgttca
     1621 cctatcttga tcaaggtgtg atttatcgta tgggagaacc taatttgtca cgccggatca
     1681 aaacacgtct cttttttgct acgactgaag agattactag tcatttctta acaactttca
     1741 ttcgaagaat acctatccga atcgaactac ctgcacttaa tcagcgtagt cgtaatgaac
     1801 ggctagaact gatttactct ttttttctcg aagaacaaag aaaaatgggg agccctttgt
     1861 cagtcagtgg accagctttg tcacttttaa caagtcagaa attatccgga aacataggag
     1921 agctgaaaaa tatcgtcaaa gtttcagcag caaaagcata tgctgaacag cgtgaacagc
     1981 ctgaaatcca tgtcacgatc catcatttgc aaaaagaact acttgcgcag cctattacta
     2041 aaaatacagc acagcaagga atctatatta cgcaagacca gaccttagag caattaatgg
     2101 agaagcaaca gcccgaacag cagcgaatcg ttaaaagttt tgagcaaatc ctaattgatt
     2161 tcaagaaaaa taactgtctg ctatattctt gcgaaggaaa attaaagcaa gaagtgattc
     2221 aattatttga ttttttgctg tttgagacca gtagacaaga aaaacacgag ctgctggttt
     2281 attggacaca atctatccga gagacctttc gacaaatgga aactgcctat caaatcaaat
     2341 tcgatggaaa cagtgtctat gccttgagtt actatctcta tcagcgaagc actatcaaat
     2401 ggttaccgga agaacatgat attatccaac taatcaaaga attagaacaa caagtttctc
     2461 agtcttatcc atctagttat cactatgtcc aacgaattct ggaattaagt aagatcaaat
     2521 tagatattga agacacaagt atggatcgga ttttactgac gatctatcta aaaaaagcga
     2581 aatggtcaaa agaaaaacga ctgccaaaag cgatgattgc tgctcacggt tatgcaactg
     2641 ctagcagtat ggcaaatgta gtaaatcgtt tattacaaga ggatctattt gaatcatttg
     2701 atatgccact agatgtcaca ccacagcaga ttgcttcaga aatccttgat tattgcgaaa
     2761 acagcgatgt ttcaaatgga ttgatcatat tagtagacat gggatctctg aaagaaatcc
     2821 atcagttttt taaaaaacag ttatccgtac cgcttttgat tttgaataat gtgacgacac
     2881 ctttagcaat cacagtaggg gaatgcctgc aaaaaaatgt atcattagaa gagatagcag
     2941 aagagactgt tcgacagatc cagccagaat ggcgactgct ctatccagag gaaaacaaac
     3001 ccaaagcact cattactact tgcttcactg gaatcggtac ggcggtccac ttaagtgaac
     3061 tgctagaaaa aagcttacct acggcttgtc agttgaaaat catcccatat gaatatcagc
     3121 aattaaaaga taagaaaaac agtgacccat tattttccat atacgaagtc gtaggaatga
     3181 ttggcaccac agatcctgat atcaccaata tcgactatct ttcattagaa gacctcatct
     3241 cgggggaaaa gatgtctgta ctagctgaat ggttggacac tactatgaat gcttcagaga
     3301 aggaaatatt caatcagcgc atcattcgta atttttcatt ggaaaaagta ctggattccg
     3361 tgactattct agatacagag aaggtgatgg gagaaatcga tcattttatg agagaattag
     3421 aggtgcagtt gaatcgaagc atcagcaatc ccaaaaaatt agcgttgtat gtccatgtga
     3481 gctgtctcat tgaacgattg atccgacaaa tgccaatcga aacctatcaa ggattagaga
     3541 aatggaagca gtgtcaaaaa gaagggttat ctgctattaa atctgcgttt agtgtcattg
     3601 aagagcatta tagtgtcatg atccccaatt ctgagattgc ctatatctat gatattttga
     3661 gtgaagatac agagtttctc tcgatagaag aagaattttg aacataagca agttgttgcc
     3721 actaattttg gcatgcaact tgctttttta gtatgtgtaa gtgaaaagag gagatgaaaa
     3781 agtggataga gaaattatct tagcttccca tggaaaattt gcttcaggga tattagattc
     3841 attagagctg atctatggga aaaatcattc tattactgta ttcgattgct atactgatga
     3901 gaattttgat ttagcggaaa cagtaaggca gctttttatg aggtacaaag ataaagaaat
     3961 cattgttctg acggatcttt ttggcggaag tgtcaacaac gaattcttac aatatatcaa
     4021 tcgggatcat gtctatctcg ttgccggatt gaatttgcca ctattgattg agctagtatc
     4081 tcggttggac gctgaagtag agacaactgt tttgatccag caggtgctaa ctgattcaaa
     4141 aaaaatgatc cagttttgta acgagcggct caatcagaaa atagaagaag acgaatttta
     4201 ggaggaagaa aaatgattgt attgacaaga gtcgaccatc ggttattaca cggacaagta
     4261 gctttttcat ggacacaaac aataggcgca gactgtattt tgattgctaa tgatgatgta
     4321 cctacgaatg agatacgaaa gacaacgatc aaattagcca agccacaagg agtcaaacta
     4381 gtgatcaaat caattgatga ctcgattgcc gcgttgaaaa gcggggtgac agataaatat
     4441 aagttattca tcgtagtgga atcgatcgag gatgcatata agctggcaga agcgtatcca
     4501 gaaatcaaaa ccatcaactt aggagggatc aaggcaaaac ctggtactcg cagcatcgga
     4561 aaagcggtaa atattttacc agaagaggag gaacgcttga aacgattaat tgacaaacaa
     4621 atagaagtag aaatccgtca agtaccagca gataaaaaag tggatgtaag agaagttttg
     4681 taagaaagga agaacctcaa tgattgtaca agcagtctta ttaggaattg ttgcatttgt
     4741 cgcacaatca gaatatgcac tagggacatc gttgctttct cgtccgattg tgacggggct
     4801 gtttacagga atcgtgttag gtgatgtaaa aactgggatc attatgggcg caactttaga
     4861 gttagcattt atcggatcgt tctcagtagg tgcttcgatt ccacctgatg ttgtgaccgg
     4921 tgggatttta gggacggctt ttgctattac agctggagcc ggtacagaaa ccgcgctttt
     4981 actaggtttg ccgattgcta cgttgacatt gatattgaaa aatatttact tagggctttt
     5041 gattccaatt atgaaccata aagcagacaa ttatgcagaa gaaggaaatt ataaaggagt
     5101 cgagcatatg cacttgcttg ccggtatcgg tctttccttg atgcttggtg ttgtggtaat
     5161 ggtttcttat atggtgggaa gcaatacgat cggaaatcta ttaaatatga ttcctgactt
     5221 cgttcaaaag gggttgtcag tagctacagg gttgattcca gctcttggtt ttgcgatgct
     5281 tgctcgtttg ctgatcaaca aacaagttgc accatatttt ttcttaggtt ttgctatagc
     5341 ttcttactta gagattcctg tgaccggagt agctattttt ggtgcgattt tagcagttgt
     5401 ggtagtcaat attatgaatg tccgtcaact accacttcaa acaactgcag gggaggtaga
     5461 agaagatgaa gaattctgag atcaaaaaca gcaaaataac gaaaaaagag ctaaatcacg
     5521 tattttggcg ttcttttcaa atggagtttt cttggaatta tgaacgtcag atgaatatgg
     5581 cttatgttta tgcaatgatt ccgattttga aaaagttata tcagtcgaaa gaagaaatgg
     5641 cagcagcttt gaaacggcat ctagagtttt ttaacacgac cccacatatc gttacactga
     5701 ttttaggaat cacggctgca atggaggaag aaaataagga tgatcctgaa tttgatgtga
     5761 ctgcgatcga tagtatcaaa acatctttga tgggacctct tgcagggtta ggagattctt
     5821 tcttttgggg aactttgcga ttgattgcca caggtgtggg tacctcttta gccatgcaag
     5881 gaaatatttt aggccctatc ttgttcattt tgattttcaa tattccgcac attctgtttc
     5941 gctatctagc aacaggttgg ggatatcgct taggaacggg ttttttgaaa aagatccagg
     6001 caaacggtat gatggaaagt ttgacattag gcgcgtctat tattggactg atggtcgttg
     6061 gagcaatgac tgccacgatg atcaccatca atattccttt aaaaatcggt tcaggagaaa
     6121 atgctgtgac tgttcaaagt atttttgatg atatcgttcc aaatatctta tgtcttggag
     6181 catttggcat agtgttttat ctattgaaga aagaagtgaa accattaacg atcttgatcg
     6241 gactagcttt ctttggaatt tttggttcgt ggatagggat tttttaaaaa aggagcagag
     6301 aaaatgttta cgatgcaaga ttatatctat gaagaaaagg aagtactaag tacgatcttg
     6361 aaaaagaatg atttttcgac aagagaacat atgaaaaaaa cagtgaattt actgatctta
     6421 gcaacgggtt catcttataa cgcttgttta gcagcgaagc cagcattgga atcttatggt
     6481 gatcttactg tcgatattca agaacctttt tattttttca attatggaaa actttctcct
     6541 tcaatcgata ctgtgattgc tgtgtcacaa agcgggaaaa gtgcttctac tgttgaagca
     6601 gtaaaaatga tacaaaaaca agggttacct gttgttgcta tcacaaatga cgtgcaaagt
     6661 ccacttgctt tagaagctgc acaaattatt gatttaggtg ccggggtaga atcagtaggc
     6721 ttcgttacaa aaggctattc tgcgactgtt ttacagttac tactcttagg gataggaatt
     6781 ggaataagta aaaataaaat cagcaaaaaa atagaacaag actacatgca acagttgcgc
     6841 aaaataatca accatttacc agcgattatt caaaaaacgg aagaattttt tgatggatat
     6901 caaagtttat ttcgtttagc acagcgtttc gtcaccattg gatatggtcc aaactgggga
     6961 acagctaaag aagcagagac gaaattgact gaaacaatcc gcgttccgtc acaaggattt
     7021 gagttagaag cttatatgca cggcccatac ctggaggcag atgcttcgca tttgctattc
     7081 tttattgaag gggaaagtgt aaataaggaa cgttcgcaaa agttgcaaag atatatgagt
     7141 cggtacgttg gagagactct caccattacc acaaaaaaag caagaaacga gaaaacactt
     7201 ggattagcaa tagaatgtga tgaatatctg tctgtattag cgcttgtcgt tccgtttcaa
     7261 ctattcgctt ataaaaccgc agtagctaaa ggtattgact tgaacaaaaa gatttttgaa
     7321 gactttgata cagtcttgaa aagtaaatta taagaggtgg aaatcatgct gaaattcaat
     7381 gaagaagaac aaatcaaagc aataaaaggc gcactagctt tacgtccgca agtagaagaa
     7441 atcatcgata aactttatgc ggaaaaattt gacgctgtct attatctagg aatcggtgga
     7501 acgtatgctt cctcgatgca agctgtcact tatatgaatg ggaaaagcaa cttgcctgtc
     7561 tttgtacagc atgctgcaga atattacact actggaaaca aaagattaac aaaagattct
     7621 atcgttattt tgtcctctgt taccggtaca actcaagaag tagtgaaagc agtagaacaa
     7681 atcaagaaag taggagcgac actgattgga tttatcgata aagcgaacag taagttgtct
     7741 cagctatgcg attttgtggt cacttatcca gctcctggaa cagaacaaat caaatttttt
     7801 atggcagcag atcgattgat gtatttgaat ggagaattcg aagcttattc agaatactat
     7861 gaacagctcg aacgttattt accaacaggg ttagtggaag cagagaaaaa agcagatgca
     7921 tttggcttat catttgcaga aaaacatcga cgtgatagta tgcattattt tattggagca
     7981 gggaatcaat ggggagctgt ttattcatat gccatgtgtt actgggaaga acaaagctgg
     8041 cttccatcga aatctatcca tgctgccgag tttcttcacg ggacgttaga gatcgtagaa
     8101 gaaacaaccc cagtcacatt atttttaggc gaagatgaac agagagtgtt atctgaacgt
     8161 gtggctaaac ttttgccaaa aatttgcagc aattatacat tgatagacac aaaagactat
     8221 ccagtagaag gtatcagtga aaaatatcgc ggacgtgttt tgtctttctt attgatgcat
     8281 gtagtgacac aacgcatcga tgctcatgtg gagcagttga actgccatcc attagagata
     8341 cgccgctact atcgtcagtt tgattattag gatagactgg ataaaaagaa tataaaaaat
     8401 aaagagagca gcgataactg ctccctttat ttttttaaaa tattggcatc aattacgtta
     8461 taaaacacat tttttgcatt atctacagtt caacttataa tctgtttaat gggacatgtg
     8521 ggtgccaacc attttttgtt atgcaatagt actatttagt tgttgcatgg atccgtagta
     8581 aaagtccaag tacaacgagt tgcgccagtt tacatagcgt tttgtatcca gaatgttgac
     8641 taaaacaaaa gttcctatgt caaccgtcaa cagagacaat ctgtccgttg caggacctga
     8701 cagatttaga aatgttcaat ctgtctctta tgtattttaa atcaatactc actaggagaa
     8761 gtttcctaac ctcctaatag taatgctggt agtaacattg tcaaaactgc ttcctcttag
     8821 gaacaagcaa agcataacga gtgtaaaaat aaaatgtaga ctattgtaac atatcattga
     8881 ttatttcaag taaaaacgat aaaagaagta aacaaacact taaaaacaat ggaaattagt
     8941 cccaacttta agaaatgtat actataatgg aaattatcaa ttatttattt ggaggaatat
     9001 ccatgaaaca aaaaatcgca gttcttggtc ctggatcttg gggcactgct ttagcacaaa
     9061 cattggcaga aaacgggcat gatgtccgta tttggggaaa tgtacctgaa cagattgatg
     9121 aaatcaataa gtatcataca aatcagcatt ttttacctga tttgacgatc ccagaatcga
     9181 ttatcggata taaagaattg gcagaagcag tggataacgc agatgctatt ttatttgctg
     9241 ttccaacaaa agcgattcgt tcagtcgcac aggaacttat tggtaaaatg aatactaagc
     9301 caatcattat acatgcaagt aaaggactag aacaagatac gcataaacga atctcagaag
     9361 ttttggctga agaaattcca gaagaaaaaa gacaggcaat tgttgtatta tctggtccta
     9421 gccatgctga ggaagtggca gtacatgata tcacaacaat tacagctgca agtattgatc
     9481 aaaaagcagc agcctatgta caaaaactgt tcatgaatga ttatttcagg atttatacaa
     9541 ataatgatgt gattggcgta gaaactggtg cagcactgaa aaatatcatt gcaattggtg
     9601 ccggcgctat ccatggatta ggattcggag atgatgcaaa agcagcaatc atgactcgag
     9661 gattggcaga aatcagccgt cttggtgtcg cgatgggtgc caatcctctg acatttatcg
     9721 gtttgagtgg tgtgggggac ttgatcgtta cttgtacaag tgttcattct cgcaattggc
     9781 gtgccggtaa cttgttagga cagggacata agctggaaga agttctagaa aacatgggaa
     9841 tggtcgttga gggtgtggca acgacaaaag cagcagtaga gctagctcaa cagctaggtg
     9901 ttgagatgcc aattactcaa acaatctata atgttttgta taatggagaa gatatcaaaa
     9961 aagcagcaaa agaaatcatg ttacgtgacg gaaaaatgga gaatgaattt tctcttcgtt
    10021 aaatagatga tcaatagtta atcgt
//
LOCUS       DACEMO010000096        10031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEMO010000096 DACEMO010000000
VERSION     DACEMO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.098"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(147..659)
                     /locus_tag="IW728_001753"
     CDS             complement(147..659)
                     /locus_tag="IW728_001753"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1025110.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(673..2970)
                     /gene="recJ"
                     /locus_tag="IW728_001754"
     CDS             complement(673..2970)
                     /gene="recJ"
                     /locus_tag="IW728_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1025111.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3112..3546)
                     /locus_tag="IW728_001755"
     CDS             complement(3112..3546)
                     /locus_tag="IW728_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1025112.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3556..4344)
                     /locus_tag="IW728_001756"
     CDS             complement(3556..4344)
                     /locus_tag="IW728_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1025113.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4341..5282)
                     /locus_tag="IW728_001757"
     CDS             complement(4341..5282)
                     /locus_tag="IW728_001757"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1025114.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5459..6046)
                     /locus_tag="IW728_001758"
     CDS             complement(5459..6046)
                     /locus_tag="IW728_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1025115.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6267..7580)
                     /gene="obgE"
                     /locus_tag="IW728_001759"
     CDS             complement(6267..7580)
                     /gene="obgE"
                     /locus_tag="IW728_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1025116.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7868..8878
                     /gene="gap"
                     /locus_tag="IW728_001760"
     CDS             7868..8878
                     /gene="gap"
                     /locus_tag="IW728_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1025117.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8963..9898)
                     /locus_tag="IW728_001761"
     CDS             complement(8963..9898)
                     /locus_tag="IW728_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1025118.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2770 a         2287 c         1731 g         3243 t
ORIGIN      
        1 attttttaaa cttctactgg tctttgcttg tttccattta taaaaaaata gcgaccctca
       61 aaagtcagat agagaatctg acttttgagg gtcgctttaa aatcgcttct ttaagaataa
      121 catcaagaat agcttctttt ttctgcttaa tattccatta atgtaatgat gtcatatcct
      181 ttgattttgt cgcgtccatg gagatccatc aattcgatca aaaatgcgca gcctacgact
      241 acaccgccta attgttcgat cagttcgatc gtcgctttga ttgttcctcc agtagctagc
      301 aaatcgtcac aaatcagcac acgttgacct ggtttaatgg catccttatg caatgtcaat
      361 gtatctgttc catattctaa tccataagtc acttcgatgg tttctcgagg taacttccct
      421 tttttgcgga ctggggcaaa accgactcct aactcataag caacaggaca tcctacgatg
      481 aatccacggg cttctggccc aactaccata tcaattcgtt tttcttttgc ataatttacg
      541 atttgtttag tagcttcccg ataagcgtca ccatctgcca tcagtgggga aatatctcga
      601 aacacaattc cttttgacgg atagtcggga atactggcaa tataatcttt taaatccact
      661 ctttgtttcc tcctaggttg ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa
      721 aaattcctca actttgattt tattgagccg tcgctggtat aatctgctct cagtcaaagg
      781 atgactttct gggctagcga ctttttgtaa aacaccgttc tctattgtaa caaatttcaa
      841 ttcaaaaaac acctgtatca taaaaattaa taatttttca gggatacgca aatattgagc
      901 tattgctttt attttatgac gtatatcaat tcgttcttga gaatggatca atttaaacaa
      961 ttttgcgtac tgttctcttg tgcctacccc attcaaatag gcctcatcag gtgaaatccc
     1021 catcagataa acacgattga atgaataaga ccgaaaaact tctttcagat ccgctaaatc
     1081 atctggacaa tccataacaa ctagagagtt tggttttctt ttttcaatta ttgcctttac
     1141 agaatctagt ccttcgaaaa caatcgcagc ttcctggata atctcaggca cgaatttcaa
     1201 cgtcgaagaa tcaaatgcta aataaaggct accttccgac gttcggctct gttcacggaa
     1261 acgttttgcc cgccaatcaa atacttgcag accacccaca gaaaaatcag ataccatcag
     1321 ctgcggtttc ttacgcccgt tccattcatt gatcgaaagc tttcctacca catctacctg
     1381 attattcagc agttcttctt cttgagtacc aaaaccaaaa gcgaccgcat caagctgact
     1441 cgatgcatcg gaaaggacaa atttcagatg ctgttggttt gctccgattc ttttgatatt
     1501 ttctgcttgg acttgtcgaa ataaaaactg aggcaaagga ttatccgtac caaatggcgc
     1561 caatagtttc agctcatcaa ttcgctctac tgttacttca tttggcagca atacttcatc
     1621 aattctcaac tcaggccctc tcatcaagtc aatttgatga tcgactatat attggttcat
     1681 cttctcctgt agtatagtga cattttcact tggcatcgtt aaaccgactg ctgcatgatg
     1741 tcctccgaag taagtaaata aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc
     1801 ttcaatacta cgtccagatc ctttagctga tccatcttct tttaaagtaa ggacaagcgt
     1861 tggtttaccc gtttcattca ttattttccc agcaacaatg cccaaaacgc cttcatgcca
     1921 tcctcggttt accaatagat ggatatgatt ctccttattg accatcgcta atgcttcttg
     1981 tgttatctgc tctacgattg cttttctttc ttcattgatc tcattcaatt tttttgcttg
     2041 aacggatgct tcctcttcat caaacgtcgc aagtaacgag acggcaggac gtggatcgcc
     2101 catccgaccg attgcattta gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc
     2161 gtccctcatt tttacgccgc tttcttcaaa tagcgcctga agtcctatcc gttctgaatg
     2221 atgaatggca tcgattccta atgaaacaag gattcgattc tcatcagtca atgaaactaa
     2281 atcggcgatc gttcctatcg ctactaaatc caaaaattct gcaggcggtt cttccaataa
     2341 tgcagaagct actttgaatg ctactccgac tcctgccaaa tctggaaatg gatagtgccc
     2401 cgctggatga cgagggtgaa caatcgcata agcgtctggt aaattctcag gtagttcgtg
     2461 atgatctgta atgatcacgt ctacaccagc ttgctgagcg tatgctaccg catcatttcc
     2521 tgctaccccg ttatcaacag tgatgatcaa ctggattcct tcttcgatct tttcttggta
     2581 taccgattgg tttggtccat acccatgctc aaatcgattt ggcaggaata cttctacttc
     2641 tgcaccaagt aattccagtg tttctttcat taccgttgca ctggtgatcc catctgcgtc
     2701 ataatcaccg taaacgagga ttttttctcc actgattaca gcttcttgga ttcggctgat
     2761 tgctttatcc atatcgtgta tcagatacgg atcatgtaat tcttgttcct ttggatgtaa
     2821 aaatcggttg atatcctttt cctcatggta acctctttgc cacagtaact taccgacaaa
     2881 tggagataat ttcagttcac ggatcatttg gagaaaggat tcagaaggct ctgtctcgct
     2941 taacttccaa tcaaaatttg cttgtctcac tttctcactc ctaacttagt tattatagca
     3001 aaggaaatca gcaaaaaaaa gcagaaagaa agaataaaaa cagatcagat ccgctagaca
     3061 gatgttcagc aggacctgat cttttgtacg accatttatg aaatatccct attagtcaaa
     3121 ataattgaag ctttgtttgg gcgattgagt tttttgttct tccggcgctt tcaattgact
     3181 gatttgtgcg gttttcgctt gtaactccgc ctgataagca gcttccaact ccgcacgttc
     3241 gtttctaaac tcgcgttgct gttcttcttt tgcttcttca attttctttt gaagttctgt
     3301 ttgatattct gacatttcct gagtcagctg tttgatttgc tttttttgct gccatagtgt
     3361 tgccgaagca gtcaaaaata caatcagtgc accaatcagc gcagaaccga tgatcactaa
     3421 gatcaatggc gcagaaaagg aagtgaagcc aaaacttaca ggaacatttt tattgttcaa
     3481 tacagcaaaa agaacaataa tcaaaactaa aacaaatcca agaatcacat tgctttgctt
     3541 tttcaattcc ttcacctact ttttgttaaa aattccgctg gctaaataat caccaatcgt
     3601 tggaaacagc gtataaaatt ttgatgcaac ttctaaaata aacggttgat tgatttcccg
     3661 ttttggatgt ctcatattgc ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat
     3721 ttgtccgact ttttccaaat agcttccgct tggatcagcc ttatcgaaga aatttgtctc
     3781 gattggacct ggattcactg tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa
     3841 agcgttggag aagccaagga ctgcaaattt cgtggcagaa taaaccgttg attttgcagt
     3901 tgccattttt ccagccatag aggcgatatt aatgatatga ccttgtcttc tttctgccat
     3961 atcgatcgca aatttttgtg tcaaaaccat cattcctaag atgttgactt caaacatatc
     4021 gtatgctttt ccaaggtcga atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt
     4081 taccagaata tcgacgcgcc cgacttcttc catcaccttt tcatataatt cgtccacgct
     4141 ttcaggattg ctgacgtcta ccttgaaagc ataagcctct ttgccgctca agcgtctgca
     4201 ctcgtctctg acaccttcta caaatgccat tcttcgtgcg caacttataa cgattgctcc
     4261 ttgctttgct gcttcataac agatttgtgc gcccagaccg cctgaactac ctgttactaa
     4321 aacaacttta tcttctaaat tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta
     4381 tcaaaatctt tcataatcgt cgtgttttca aacacttctt gtgcttcttt ttcaagctct
     4441 aaagctgctt ttcctaaata tctcgcactg atatgagtaa gtagcagccg ttttgcctgc
     4501 gcttcttttg caacttcagc agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt
     4561 cttgcttcat ctttattaaa tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc
     4621 acaacggaat tatgtgtttt tctcgtatcg cctaaaatag tcacgattcg tcctttttta
     4681 tctggaccta cgaaatcttt tccattgatc gtccgcccat cttctagttg gatcgtttca
     4741 ccttgcttca gtttgccgta cagtggtcca gctggaatac ctaattcttt tagacgatcc
     4801 acctgcaatt ctcctttatg atcatgttca accacacgat aaccaaaact gtcgatccca
     4861 tgatttagtt tttttgtata aacagaaaat tgctgatcag taaaaatcgg ggctgtttca
     4921 tttagctcaa tgaatttcag cggataactt aaacgcgttt gcgaaatacc taaagagact
     4981 tttatgtatt cctctatccc ttttggcccg taaatctcta atggcgtatc cccaccttga
     5041 aatgaccggc tgctgatgag cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta
     5101 atgaaaattt taccgatttt tctcggacga atagtcgttc gcaaaatctg catttgcgtg
     5161 ccttcgccac agtcgaacaa ccaaacttca ttgcgctcgt ctaaaagttt caatgctata
     5221 cttgtcacat ttctatgttt tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc
     5281 ataatctacc tgactttctt taagttcctc tttaatttta gggtaaaaag aagggataat
     5341 caaccgtaag tcgagaatgg gttcaaatta aaaataacgt tctaaagcga tagcgttttc
     5401 tttgaaaatt actataaaaa cagcagcgaa cccgtttgag atcctctgct gttttttatt
     5461 aatttgaaga tccagaactt tcattgtcgt tggaagtttg cggatgtttg tccttccaag
     5521 tcttttgtgc agcttctttc gcatgatttt cagcttcttt cgcatcatgc acatcttttt
     5581 cgacatcttc ataatccaag cgagtaattt ctccgcctaa ttccatcatt accaattgat
     5641 tcagtgggcg attgtcctct tcatcagcaa tcaagatcag tcctgtagct ccttcaccga
     5701 ttttatttcc tacaaagtcg aacacggtct gggcatcttt tatttctttg gcatcttgtg
     5761 tcgcaccgat cattcctccg gcaaaccatc ctaataaaat ccctagcgga ccacctaata
     5821 caccaaccaa cattccgatt aatccacccg tagaagattt gttattaccg gtaaagtcga
     5881 taaaatcttc tattttgaat tgatgtgtcc cattaggatc atggctgaca actgccatct
     5941 gttctccttt aatcgaacga acagcatgta actttttgat ttctgaaaat gcctgatatg
     6001 cctggctttc tacttgaaaa tttaaaatga ttactcgttt gctcattaaa aacgcccctt
     6061 tcataccttc ttgttattta ttttaccttt aattgaaaaa taagaaaaga aaagcactct
     6121 ttttttccaa taaaataggg ctgtgacaaa agtcgtatca cagctccttt ttccgaataa
     6181 acggcgttct tgaagctgat cacttctgtc acaacctccc ttaaatctga atctatattt
     6241 tttggtattt gtttttattt gcttgcctat tcgacaaatt caaattcaaa ctcaccgata
     6301 cgtacaaggt cgccatcttt cgcaccgcgt gcccgaagag cttcatccac acccatacca
     6361 cgtaattgac gagcaaaacg catcactgtt tcatcatgat cgaaattcgt catttggaat
     6421 aatttttcga tcttctcacc agataagatc cacgttgcat ctgaatcacg atcgatttgg
     6481 aactcaggac cttctggatt gaatccatag tgtacagttt cttcttccag ttcttcttcg
     6541 tacaatggga attctggcgt tacttctaac aaatctgctg tagcattcaa taacgcgtcc
     6601 aatccttgtc gagtcactcc agaaattgga aatactggga tgtcatcagc aaattcatct
     6661 tctttttcct tgttcagctg ttctttgaat ttcactagat tttccggtgc atccggcata
     6721 tccattttgt ttgccacgat gatttgcgga cgctctagca agcgtaagtt ataagtagat
     6781 aattctttgt tgatggcaag atagtcttca taaggatctc gtccttccat cccactcata
     6841 tcgatcacat gtaaaattac tcgtgttctt tcgatatgac gaaggaattg tgtacccagc
     6901 ccaacacctt gcgaagctcc ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg
     6961 ccatcacttg tagtaaccat tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg
     7021 atttttggac gagcagatga aataacggat agtaaagtcg atttcccgac agatggaaaa
     7081 cctacaagtc caacgtctgc caatactttc aattctagtt cgatttttcg ctcttgtcct
     7141 ggttcaccgt tttcagcaat ttccggtgca ggattccgag gagaggcaaa acggatattc
     7201 cctcgtccac cgcgtccacc ttttgccaca actagtgttt gaccattttc aattaaatct
     7261 cctaaaagag caccagtctc cgcatctcgg acagtggttc cttgtggtac tttaacatag
     7321 gtatcttctg aaccacgacc gtgcatccct ttactcatcc cattttcacc tggctgcgct
     7381 ttgaaatggc gattgaaccg aaaatccatc aatgtacgta acccttcatc gacgatcaag
     7441 atgacatcgc cgccacgacc accgtctccg ccagccggtc cgccgtcagg aacgtatttt
     7501 tcgcgacgaa atgcaaccat tccgtctccg cccttgccag ctttcacatc gatcgttact
     7561 tgatctaaaa acatggacat aattaagtcc tcctattatt catttattta tttgactgcc
     7621 tgaatttcca caataaacaa aagtcttact tattttatcg gtgaaatctg attttgtcta
     7681 tcatttcagc aaaaagattg tttaccttct tcttttttta tcatttggta aggtttccaa
     7741 ccttttttct gctaccaagt ataatttttt gacaaaatat tctttgcgta gcagaataac
     7801 agtgtattaa ttgtattaat tgaggatgcc gtttcacagc acccctaata aaggaggaac
     7861 ttttgatatg acagtaaaag taggtattaa cggatttggg cgtattggcc gtctggcttt
     7921 ccgccgcatc aaagaagtct cagatgatat tgaagtagta gcaatcaacg acttaacaag
     7981 tccaactatg ttagcacaat tattgcaatt tgactctact catggtactt atcctggcac
     8041 agttactgca acagaagact caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga
     8101 acccgacgca agcaaaatcc cttgggcaaa agaaaacggt gtagatatcg tactagaatg
     8161 tactggtttt tatacatctg aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg
     8221 agtagttatt tctgccccag ctggtgctat gaaaaccatc gtctataacg tcaatgatga
     8281 tacattagat gaaaacgaca aaatcatctc agcaggttca tgtacaacaa actgtttagc
     8341 acctatggct tatttcttaa ataatgaatt tgggatcgaa gttggtacaa tgacaacagt
     8401 tcatgcctac acttccacac aaatgctctt agatggacct gtaaaaggcg gtaatttgcg
     8461 tgctgctcgt tcagctgctg acaatactat cccacattct actggtgctg cgaaagcaat
     8521 cggcttagtt atccctgagc tacaaggaaa attacaagga cacgcacaac gtgttccagt
     8581 cgttgatggt tcattaactg aattggtttc agtattgaaa acaaaagtaa ctgctgatca
     8641 agtaaacgaa gcaatcaaaa aacacacagt tgacaatcct tcattcgggt atgatgaccg
     8701 tcaaatcgtt tctagcgatg tcattggaac aactcaaggt tcgatctttg acccaacgca
     8761 aacagaagta acaactgctg gtgatttcca acttgttaaa actgttgcat ggtatgacaa
     8821 tgagtatgga tttacttgcc aaatgattcg cttactagaa aaattcgcta acttgtaatt
     8881 tgatagatca atcgataaaa gaagcctttg actggatcca gtcaaaggct tctttctttt
     8941 catcccctac ttttaaactc gttcaatgaa catattggct taaaaattgt tggactactt
     9001 gctgatattt ttcaggatct gtcttgtatg cttccgcatg ctcggctcct tttacaacat
     9061 acttttcttt tggtcccttc gttgcacgat agaccttatc taacatttca aatggtacaa
     9121 atgtatcttc gtctccatgt ataaagagca ttgggcgctg gtttttcttc aattgttcca
     9181 ctgcatttgc ttcaccgaaa aagtagccag cacggacttt tgtcactaga ctcgttactt
     9241 gcataagcgg aaatgctgga agactaaaca tatcgtctag ctgataagca agctcatcat
     9301 gaacggaaga ataaccgcaa tcttcaatga ttgctttcac attatctggt agtttttctc
     9361 cgctagtcat cataactgtt gctgctccca tgctgacacc gtatagaacg atttcttgcg
     9421 attttccatt gttttctagc actttgttta tccactgtac atagtcttta cgctctggcc
     9481 atccaaatcc aatgtaatcc ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga
     9541 cattgtaacc catatcatga aacatctttg cgtaattggt catcgtttca gcgtttccca
     9601 tatagccatg agccacaaga gctgtttttt cacttttcgt ttcagcagga agataaatcg
     9661 ctgataattt cagaccgtct tttgaagtga tggtccaatc gcttcggttt tcttttgaac
     9721 taaaccattt atgtgcatcc gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt
     9781 ctaaaaagtc tttctcagaa ggcacaacag cataattata aaaataattc cctgcaaaca
     9841 ctaacgcaac aatcagagct aaagcgacgc ctaaaaggat tttcatccaa agtttcataa
     9901 atcatccatc tcatttctct tttttcatat aaccttgaat tagtataatc aaagaatctc
     9961 tactatgcaa tgttcatttt tataacccgc gaattgtaaa attaagttag aaaaataaaa
    10021 aggcatttat g
//
LOCUS       DACEMO010000097        10031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEMO010000097 DACEMO010000000
VERSION     DACEMO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.099"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            256..2091
                     /gene="tdc"
                     /locus_tag="IW728_001762"
     CDS             256..2091
                     /gene="tdc"
                     /locus_tag="IW728_001762"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1025119.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2396..3874
                     /locus_tag="IW728_001763"
     CDS             2396..3874
                     /locus_tag="IW728_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1025120.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3840..5171
                     /locus_tag="IW728_001764"
     CDS             3840..5171
                     /locus_tag="IW728_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1025121.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5285..7837
                     /locus_tag="IW728_001765"
     CDS             5285..7837
                     /locus_tag="IW728_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1025122.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7844..9283)
                     /locus_tag="IW728_001766"
     CDS             complement(7844..9283)
                     /locus_tag="IW728_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1025123.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9629..9991
                     /locus_tag="IW728_001767"
     CDS             9629..9991
                     /locus_tag="IW728_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025124.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3185 a         1650 c         2131 g         3065 t
ORIGIN      
        1 ggacagtcat tttatttgtt tgttcgcttt ttttaggtta tctattttag aagactgatt
       61 tattgaaaaa ttgttcgctt ttttgcgttg aattatcaga aaaatccgac tttatttatt
      121 tttgtttgta aaaatctacg tggatttgat gttataaaaa aaaaattctc atttttggcg
      181 ctatagtgaa gaggtgttac agataaacac atgtatctgc aagacattga ccaacaaaat
      241 atggagggaa gaaaaatgaa agatatggat atcaaggccg tctttatagg cgacaaagct
      301 gaaaatggac cagtttataa aatgttgttg aacaagatgg tagatgaaca tcttggatgg
      361 agagaaaatt atattccttc agatatgcct gcaattagcg aaggagataa gctcactcca
      421 gattatctag ccacccgcga ccatatgata gaggtcttgg atgaagtcag tcagcgatta
      481 cgcgcaggat cgatcccatg gcattcagca ggccgttact ggggacaaat gaatgctgaa
      541 acattgatgc ctgctttatt agcttacaac tatgcgatgc tgtggaaccc aaataatgtt
      601 gcattggaat cttctatggc aacttcgcaa atggaagcag aagtaggaca ggattttgct
      661 tcattattca atatgacaga cggatgggga cacattgcag cagatggttc tattgctaat
      721 cttgaaggat tatggtacgc ccgttgcatc aaatcgattc ctttagctgt caaggaagtg
      781 ctgcctgaaa aagtgaaaaa aatgtctgag tgggaacttt tgaatttatc tgttgaagaa
      841 attttggaaa tgaccgaaag ctttacggac gaagaaatgg atgaggtcaa agcagcttct
      901 tcccgtagcg gaaaaaatat ccaaagatta gggaaatggc tggtccctca aacaaaacac
      961 tattcttgga tgaaggcgtt agatatttgc ggagtcggct tagatcagat ggttgctatc
     1021 ccagttcaag aggattatcg aatggatatt aatgctttgg aaaaaacaat tcgtgaatta
     1081 gctggacaaa aaatcccaat tctaggtgtg gttgctgtcg taggaacgac agaagaaggt
     1141 caagtagaca gtgtagataa aatcgtccaa ttaagagaaa ggttaaaaga tgaagggatc
     1201 tatttctatt tacatgttga tgccgcatat ggcggctatg cgcgttcact gtttctaaac
     1261 gaagcaggag aatttgtgcc gtatgcttcc ttagctgaat tctttgaaga acatcatgtt
     1321 ttccaccact gcgtaacgat tgacaaagag gtatatgaag ggttcagagc tatttcagaa
     1381 gcggattctg tcacgataga tcctcataag atgggctatg tcccttatgc tgctggagga
     1441 atcgtaatca aacataaaaa tatgagaaat atcatttctt atttcgcgcc atatgtgttt
     1501 gaaaaatcag taaaggctcc agatatgtta ggagcttata ttttggaagg atcaaaagct
     1561 ggagcaaccg ccgccgcagt ctggacagca catcgtgtat tgccattgaa tgtgaccgga
     1621 tatggtcaat tgattggggc ttcaatcgag gcagctcaaa gattcagaga atttctcgat
     1681 catctgactt ttactgttaa aggaaaaaca atcgaagtct atccattgaa tcatcctgat
     1741 tttaatatgg tcaattgggt attcaaagaa caaggctgta cagatttgaa cgctatcaat
     1801 gaattaaatg aaaaaatgtt cgatcggtct tcttatatgg atggcgatgt ttatggtgaa
     1861 cggtttatta cttctcatac tacatttaca caagaagatt acggtgattc tccgattcgt
     1921 tttgttgaaa gaatgggtct aacgaaagaa gaatggaaaa aagaacagaa aatcactctg
     1981 ctacgtgcgg ctatcatgac tccttatttg aatgatgatc gaattttcaa tttttataca
     2041 aaaaaaatcg ccaaagcaat ggaaaagaaa ctaaacgaaa tcatccaata gaactgattt
     2101 ttgtgtgaaa gaataacaag agacatatgg agaacagaca attatttgct tcatcaaaac
     2161 aattgtattt ccccctgttt ttcatatatt cctcgccaag atattctttt ttataaaacg
     2221 ttctataagt tggatcgttc gtttcatgta tcaaacccag ttcactaaga tcgttcaatg
     2281 gatgtttcgg tgatactggg ttttgtactg aatcgatgaa accttgtcat ttctgttctt
     2341 gaacaaaaat aaaagaaaac aaaaacatat gtcgctattg aaagaaggaa taaagatgaa
     2401 agatactggt tcatcaaagc aaatcacgac catgaccttt atcggaatga cctgcgccct
     2461 tgtagcaagt gtacgaaata ttccagatgt agcagcaacg agctggacga tgatttttta
     2521 catgctggtt gcagtattat tgtacgcttt tccaatctca ttgatctctg gtgaatttgc
     2581 tggaatgttt ccccaaaaag gtggtccaga actttgggta tcgaatgctc taggaaaaaa
     2641 atgggggttc gttgtctcat ggctgttatg ggtccaaatg tttcctggga tggtgatggt
     2701 cgcttcagca ctcgctccgt tgtttggtaa tattatcgat aatgtgccgc ttggtttgaa
     2761 cagcaaattt acattagttg tcattttagt cgtttattgg atcatcactt ttttgaattt
     2821 gaaatttgat atggcaaaaa tcggcggaaa agtcggtgtt tggttaggat tgtacgtgcc
     2881 tttgacgatg atgctcttat tagggtttgc tgcgtggatc aaagttggga tcgtaccaac
     2941 cggaacatta ggcagttttt catgggacaa attaatacca gatactacga ctgcatcctc
     3001 atttgtttat tttgcgccga tcatgtttat ttttactggt atcgaaatgt cctctgtgta
     3061 tatcactcga ttggaaaatc cagttaaaac gtatattcgc ggagtatttg ccgcgttaat
     3121 ttttatattt ttcgtcaata ttttgaatgc tttagttgta gcaaatgtcg taccaaaagg
     3181 acaaatggaa ttgaataata tcgcgcaatc tatttcgatt tattgccaaa ttcttggttt
     3241 gccacatctt attgtgaatc tgtttagttt acttgtcttc attggtgtag cagtccaatt
     3301 atctgcttgg gcctcaggac cagcaaagac agtgacagaa agtgctagaa aaggtgcgta
     3361 tccaccaaaa ttcaattttt ggaagacgaa tcaatttgac gtatccaaat ccgttatttt
     3421 gactcagtct gtcattatct caatctttgc acttttttat ttgctgattc ctggcgtcaa
     3481 tcaagcattt ttgatgctcg taaattcaac tactgttatc tactgtatcg tctatgtcat
     3541 catggggatc gcagttttga gattgcgtta tacacatgca aagttgaatc gaccatttag
     3601 aattggaaaa aaagagaaaa agagcaatct tggagtttgg actgtcgtta ttgttttatt
     3661 tgcggcaatc ggattttctg tcggactgac gatgaaagct ggaacatgga tcaatctaat
     3721 tgcagtcaca gccatcagtg tcattttgtt tgtagtacca ctctgtattg aaaaaataaa
     3781 gaaaccttct tgggaacagg aagtcgccat gctgcttgaa aaagaaaaag gtgaacttga
     3841 tggaaaaacc ggcaatgaaa cggactctaa ctaatcgaaa tatccagtta atcgcattag
     3901 gtggcgcaat tggaacagga ttatttttag gttcagcaaa ggcaattgca tcagcagggc
     3961 cagctgttat tctcgtttat ttgatatgcg gtttggctat ttatggtttg atgcgggcca
     4021 tgggtgattt atttttagca aatagtacat ttaactctat cagtgatttc gttgactatt
     4081 atttaggcaa aaagtgggca ttttttgtca attggactta ttggctttgc tggcttggag
     4141 ctggacttgc ggaattaaca gcaataggat tgtatatcca tttctggttt gtacatgtac
     4201 cagctttgat aagcggaggt agcgcactaa tgatcttaat ggcagtcaac cttgttgcag
     4261 tcaagtggtt tggtgaattg gaatcttttt tctcaatcat caagatcttg gggattttcc
     4321 tttttatctt attgggtata ggattgagtg tctcagattt tcatacccag tctatcggaa
     4381 tcaaagaaaa ttttggacag atcgatgaat tactaccgtt tggctttgct ggaatggtag
     4441 tctcatttcc tatggtttta ttcagttttg ctggaattga aatgatcggc ttaaccgttg
     4501 gcgagacaag tgatccgcaa aagaatttga aaaaagcaat caactgtctg ccttggcgta
     4561 ttctcttttt ctatatagga acgattttgt cgattttatt tgttgttcca tggcattgtt
     4621 tagatttaaa agaaagtcct ttagtgacta tgctgcaagt gatccattgc caaggcggag
     4681 cagcaattgt caatctaatg attttggttg cttctttgtc ctcagcaaat agtgcagtct
     4741 acagtactag caggatttta tatcaaacag caaaagaaaa aagtttgccc cattggtttt
     4801 gtgcgctttc taagaaaggc gtacctttac acggtatttt aattacagga atgttatttt
     4861 ttgccggtct ttgtctacat ttttttgtag ctgatagtcg attgtttttt cagcttttgg
     4921 caggcatgac aacgagttgt tttctatttg tgtggtcttc gatcttgtgt gcacatgttc
     4981 aatttattaa agaaaagaaa cggaacacta cacaaagata taaagatctt gctttgttgg
     5041 tgttttttac ttcaattgct ttcagcttgt tattcgaaag cacgacacgc tttattcctt
     5101 ttttattggt tatctggttt gtattcttaa attttgtata taaaagaata agcaatagaa
     5161 atatcagtta ggcccagata cattttgcaa aacgaattat ttcagacccc cctgaaataa
     5221 tcgatacgcc tgaaaaaact tccctgcgta taataacagt atcaattaaa caggagggga
     5281 taggatgaac tggtacagat tgacggtaga agatgtattg aaaaactatc aaacatcaac
     5341 agaaggcttg tcgcaagaag aacgacagaa acgcctgact gatcaaggat ataatgaaat
     5401 aaaagcaagg cagcagacaa aacgatggaa aaaaatagca aagcatttta ctgatctgct
     5461 gatgatcgtg ttgatcattg cctctttttt gaaatttgct tctagtgaat atattgaagg
     5521 aagtattatt ttattcgtag tgatcgtcaa tggactcgtt agttactggc aagaaagaaa
     5581 agcggaagaa tcattgaatg gtttgaagca gctgatggga caagaagcga tagtactatc
     5641 aaacgggatg caagaaaaaa tccctgcgaa agaactggta aaaggagatg tcattgtctt
     5701 acgtgccgga gatgtcgttc cagcagatat ccgattaatc gaagcacatg atttattagt
     5761 ggaagaatcg attttgacgg gagaatcaga agcatctgaa aaaagtcatg ccgttttgac
     5821 agaagaagaa tcgatcggcg accagaaaaa tatgggtttt tcaggaacgc ttgtacaatc
     5881 tggttctgca gtcggtgtcg ttgtagaaac aggaaacacg acagaaatcg gtaaaatcaa
     5941 tcaagcattg caatcggtcg aacaacaaac gacgcctctg attaaaaaaa tcaaccgatt
     6001 aaacaaacaa atttttcaag gcattctttg tttgagccta ttcttagtta tttttacgac
     6061 atttcgatat ggtatggatt ggcatatcct tctttcggct acgattgctt tggtggtgtc
     6121 aatggtaccc gaaggattgc cagctgtttt gaccatgatt ctatccgttg gagtacatga
     6181 aatggcaaaa gaaaaggcga tcatcaaagg acttccttcg gtagaaacat taggttctat
     6241 gacagtgatc tgttcagata agacaggaac attgacaaaa aatgaaatga cagttgtgga
     6301 tgtagctgca aaagaagaag cctgcgtgtt gtccattatg aaaaactgtc aggaattaaa
     6361 aacaaaagaa gaacagaaaa cagaaaattt aagtggcaat ccgacagaag ttgcgctgat
     6421 tcgacacaca gaaacagcca atctaccttt gaaaaaaata gaagcaaaga ttccgttcag
     6481 ttctgaatac aaatatatgg caacgatgca tgcggaggaa aaaggagcgg tgatttacgt
     6541 caaaggtgca ccagaagtat tatttgctaa atcaactctt tcttctgcag aacaagaagc
     6601 atggagtcag acagctgcag aatttgcgag aaaaggtcag cgtgttttag gatttgctta
     6661 taaaaaagta gattcgaaac aggaactcac gcatgagact ttaacccaat taacttttgc
     6721 agggatcgcc ggattgatcg atccgccaaa agaaagtgca gtcaaagctg taaaggaatg
     6781 ccaacaagca gggatctcag tgaaaatgat tacaggggat cacaaagaca cagcaaaagc
     6841 gattgcggaa caaattggct taaaacatac tgccaaagtt ttagaaggga tcgatttgga
     6901 tcttatgtct gatgaagagt tgattcagca agtgccaata gtcgatgtct ttgctcgaac
     6961 gacaccagaa cataaactgc gaattgttaa agctttgcag aaaaatggag aaatcgtcgg
     7021 aatgaccgga gacggcgtaa atgatgcacc tgctttgaaa agatccgatg taggaattgc
     7081 tatgggaatc aaaggtagtg aagtaagcaa acaggcagct gatatggttt taggagatga
     7141 taatttccat acgattgcaa aagccgtaaa agagggccgg cggattttgg ataatttgca
     7201 aaaaaccatc aatttcttct tgccaactgc attagctcaa ggattgatct tgatttgggc
     7261 acttatgctg aatcgcccgc tacctttgtc cccagtacag attttatggg tcaatatggt
     7321 aacgacaatc actttatctt atgcactagg gttcgagtcg ccagatccag aaatcatgaa
     7381 gcgcccacca agagatccaa agcaaggaat cctatctggt tatcatttgt ttcggatcat
     7441 ctatgtctcc ctactgatca tggttcctgc ctatttattg gctatgcagt ttgaagggca
     7501 aagcttacag caaaccattc ttctgcaaaa tatcgtctta gctcaagctg tttacatgat
     7561 caattgcaga gagttgtcca agccgtcatt gaaccaaggt cttttgcaaa acaaagcgct
     7621 ttttctttca ttaggtatcc ttgctttatt gcaagggatg gttatttttc taccagtcgg
     7681 ccagcagctg atcggtacag taagtttaac gatgacacaa cagttattga ttggagcaaa
     7741 tgtgattctt ctattcttag ttgtagaaat agaaaaaggg atcatgaaca aactgtttcg
     7801 caaaaaagaa aaaacagcta gagaatacag tcgctagccg ttactataaa aaagttttga
     7861 aagtgagttc agtcaaacga ttgagctcat ttttcgttgg aatagaagaa atgtatacaa
     7921 tagggatatc tagagaatct agagggaaat tggaaatcaa gatatctgtt ttttcaaaag
     7981 aaatagtgga aagatcggtt agttcgatag gtaagaagca gacacgtttt cctaagtaat
     8041 cttttaattc ctgctgcaag aacgccttcc atgaaggttc tccttggaaa ataaaataga
     8101 cattgaacaa attcggtttg atagaaaaaa tcgtttgttg gatcagtaga gacaagagtt
     8161 ggagaaagta atttggttca ttgatttcta tttctttttt tagatcgttc agtaaattct
     8221 ctttctgttg atacaagtgt gcatattgct gatcatatgt ttctaaaaaa cgatggtatt
     8281 gacggtccaa tgcttcaatc aatatcggcg attcatgata ttttaatatg aggagagcca
     8341 agttatcagt cagagatgtt ttttttagtt gatcaagaga cagctctgaa gctagttcct
     8401 ctacaaacgt ttcgaggacc tctaaaaatg gagaatgatg caggcagcga gcggtttctt
     8461 gtccatagtt attgttgtaa ttccaacttt ccagaaagca gaaagaagca aaagaaagct
     8521 cagtgtcttt aagataaata ccttccgtta aatacaattt tttcaatgca gagtcataca
     8581 gggaggagag tgtgctgttg gtatggatag aagaatctaa tcggcaattc gcttttatac
     8641 ggatcgtatt gataaagaac caacatgttc cgaaaatctc ttctgtttca accgctagtt
     8701 cagtttgact gaggtttctt aaaaattgaa aataatggga ttcatggatc gaataatcct
     8761 cgaaaaaata gttgttatga gtgaaaggaa tcagcaatcg atgataaaaa gtacgaatcg
     8821 cacgctcttt tccaagaata caagctgtat atttcgtcaa atcgatctgc aagtcgtaac
     8881 ttgctaaacg ctgattgatt ttagtcacat gtcttttcaa tgtcccataa gaaagcccat
     8941 ggtcggctag aaaatcaggg acagcaactt tttttgtaaa aagcagattt ttcagcagtt
     9001 cgacactgac tgattgtttg aaaaaaatct cccaaaggtc atttgttaat aaattttgct
     9061 ggttttgcaa actgattcct gcttgatcgc tgatgatgtc ccagccttcg ggcagctgag
     9121 agcgaataga ttgaaggtca gaaaaaatcg ttcgttcggt tgtccctatg atttcagcta
     9181 attcatttac ggttatcttt gtatgattca gcaattgttc tactaaagaa acatgacgtc
     9241 gaatatcttt ttcggtaatg atttttttca ttagtgaata catcaatcag actcccttct
     9301 attctaagaa tacggagaaa ataaacggtg gtcaagtttc aactgatttt tcaatcattg
     9361 tttatttttc tggagcattt actgatgata agaatttcat catgaacttg aaaaatgata
     9421 ccactaaaat caaaaaatag gtgggcaaac aataataaac attttaatag tagatggtag
     9481 caagaccaac acaggcggat tgaacattta agagttgata aaaaagaaaa atatcgaagt
     9541 atttgattag caactacttg tattcatttt tctattctta cgtggtatct ttgaataaag
     9601 cgaatcaata agaaagagat ggaagcagat gctaacgaaa aaagaactaa tcgatcacat
     9661 caatgaaaca tacaatttgt ctccagaata tatttttaaa aaatttcctg attattgcgt
     9721 attcagacat caaaaaagca aaaaatggtt tggggtcctg atgtctgttc cagaagaaaa
     9781 aatctatgat gatgcaagtg accataggat catcaatatt cttgatttga aggtcgatcc
     9841 tgaacttggc ggaattttgc gaggaaaaga ggggtatttt ccagcctatc acatgaataa
     9901 agaacattgg atctctattg atctacgtaa aatagaaaag ctagatgaac tagcagattt
     9961 gattgatgga agttttaaat caacgcaatg aatgtctagg acaatagaga agaggttgtg
    10021 acagaaatgt t
//
LOCUS       DACEMO010000098         9978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEMO010000098 DACEMO010000000
VERSION     DACEMO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.100"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(202..2322)
                     /locus_tag="IW728_001768"
     CDS             complement(202..2322)
                     /locus_tag="IW728_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1025125.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2552..3730
                     /locus_tag="IW728_001769"
     CDS             2552..3730
                     /locus_tag="IW728_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1025126.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3746..4504
                     /gene="aroD"
                     /locus_tag="IW728_001770"
     CDS             3746..4504
                     /gene="aroD"
                     /locus_tag="IW728_001770"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1025127.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4527..5012
                     /gene="ybaK"
                     /locus_tag="IW728_001771"
     CDS             4527..5012
                     /gene="ybaK"
                     /locus_tag="IW728_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1025128.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5083..6336)
                     /locus_tag="IW728_001772"
     CDS             complement(5083..6336)
                     /locus_tag="IW728_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1025129.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6453..7802
                     /gene="gdhA"
                     /locus_tag="IW728_001773"
     CDS             6453..7802
                     /gene="gdhA"
                     /locus_tag="IW728_001773"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1025130.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7914..9260
                     /locus_tag="IW728_001774"
     CDS             7914..9260
                     /locus_tag="IW728_001774"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1025131.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9568..9954)
                     /locus_tag="IW728_001775"
     CDS             complement(9568..9954)
                     /locus_tag="IW728_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1025132.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3182 a         1810 c         2040 g         2946 t
ORIGIN      
        1 tctagtaact ctttggtaaa ctctgaatag gacatcttga aacactcctt tgaatggttt
       61 tcgtcgactt tattctaaag gatttcaagg tgtctgtttg cgtttattag aaaaaagaaa
      121 aagtattggc aacagaaggc gtcaccaaca ctagatatta tagatcctaa ttttgtcgct
      181 gctcctttta aatttatttt atcattcgga agaacttgtg gtcgttccgt gaagttcttg
      241 gtacgtttgc gttttataaa gatcaacagt tgattgtccg ttgtgttcac taaagacact
      301 agttgacttg tcaccaagag atttttcagc ttcttttaat ttctctaagc cattcttgta
      361 gtcataatca ctggtatcaa ttgggtctaa tccactattc gtatagaacc gaagaagatc
      421 accattattg atttggtcag aaacagctaa ctgtttatca actttttctt tcagtgaatc
      481 aatttcattc tgtacttcct cagtaggttc agtaatagct tcaccagttt cgttactata
      541 gatcgtacca ccgtagtaag tatattttgg tgtaacaaaa tcaccatcac ggaaagaaac
      601 aagctgttca tgatctttcg agaatagatc ttgtcctaac tgaatataat tttttgtatc
      661 tacacctagt aagtgaagta atgtaggcaa ggcatcaact tgtccgccgt aagtgtgatt
      721 gattccgccc tgtgtttgtc cagggatatg gatcatataa gggacacgct gcatttgtgc
      781 attgtcgtag tttgtccaag tagaacttgt ttttccaact aattcagcta gatttttatt
      841 tcttgaattc gagacaccgt aatggtcacc gtaaaggacg atgactgaat tatcatacaa
      901 gccgctttct ttcaaataat tgaagaattc ttgtacggcg gtatctaaat aattggctgt
      961 cgcaaagtaa ccgttgatcg tttcatcatt tgtattggct aacgggaatc ctgcttcgtc
     1021 attggtgaat tttgaataag gataatggtt agatacagca atgaattttg aataaaatgg
     1081 ttgttgcaga cgttccaagt attgtacgga ttgttggaaa aatggtttat catgcaaccc
     1141 gtattggaac gaattgtcat cattaacatc ataataagaa gaatcaaaga aataatcata
     1201 tcctaaattc ttgtatgtct cgtttcgatt ccagaatgta ccagcgttcc catggaatac
     1261 cgcactaatg taaccttgtg tttgacttaa aatatcaggt gcagcttgga aagtattttt
     1321 tccgcctaat tgtgtgaaca aggagccttg attcaagcca aacaatgaat tttccatcag
     1381 cgtttccgca tcgcttgttt ttcctgcttt tacttgatgg aaaaagttgt caaaactgaa
     1441 cgtgctgttg ctgtggtaaa gactattgat gaaaggtgta acttcatgct ggagaccatt
     1501 ctcatctgtc aatttataat cgatcaggaa ttgctggaag ctttctaaat ggatataaat
     1561 cacatttttt cctctagcga gcccgaacaa atcgtcattt ggtgctgcat aatgttcttt
     1621 tacataattt tctacttctt tcatatcatt gggacttgct tctgcccgca cttggtttgt
     1681 ctggtaagtt tgaacaccgt cataaaccat gaaagcattc aagccaagat atttgaccaa
     1741 ataatcacga gagaatgttc gagtcagcaa ttctggacga tcagcttccg ccataaaaag
     1801 atttcctgag aaaatcataa ctgctaatgt agagatcgca aaagccattc ttgctcggat
     1861 cgggcgaggg tccaacttga ttttttttgc aatcagcaga acagtcaaga taataaaatc
     1921 gatccaatag atcacatcat atgggcgaaa cagtcgaatg gcactttctc ccagaccact
     1981 cgctactttc ccagcaccta aaatcgtgtt gatcgtaatg aagtccgtga attctcgata
     2041 ataagtgaca ttcgaaaaaa caagcaatgt caaaagaaaa taaatgacca tcatcgtgat
     2101 ataagcaact ctagggcgtc tgatataaag agcaattcca agtaataaca atgtagttgc
     2161 aatagggttg ataaacaaga taaagtattg aatgccactt tcgattccga gatggaaatc
     2221 aactaaatag gcaaaaatcg ttttcaacca aagtaaaacg actagtaaag aaaaaaatcc
     2281 gagcctctta ttcaaaaagg caggtgtttt tatacatttc aagataatct tcctttctgc
     2341 acttcttaat cacagaagtg tcctgaaacc atgttacaca gctcacttat tctcatttat
     2401 tttactagta tgacaatata gtgtcaattg aaaccagaag tttatgctgt ttcattaagg
     2461 ttttctttaa gcttcatgaa aaaatgagca gtaagacaag tcctatgttt tttgttatac
     2521 ttgggaagag aaagctaaag caggtgaagg aatgaaagta aaagtaaaaa aaagagcaag
     2581 caatcgattg aaaaaaggat atccactgat ccaaaaagaa gaccttgctc atccccaaaa
     2641 agaaaccacg caatgggtgg aattagttga ttcgcaaaac caatttatcg caaaaggata
     2701 tctaggaaag cagaataagg gaatcggttg ggtattgagc cagcagaaca tcccatttga
     2761 tcaatctttt tttgaagaat tttttgtgaa gtcaaaagaa aaaagaactt tctttttttc
     2821 agatgattct actaccgctt ttcgtatatt caatggagaa ggagacggga tcggcggtct
     2881 gattgttgat tattatgcag gttatgctgt tttttcttgg tataacgaaa cgttatatgc
     2941 acatcgtgaa ttattgattc aagcttttcg tgaaatctac ccggaaatca taggaggcta
     3001 tgagaaaatt cgatttgaat cagtaaatct gcctgagagc cagttcattt acggaaaaga
     3061 ggcaccggaa cccttgatcg taaaagaaaa cggagtaaaa tttgccgttt atttaaatga
     3121 aggattaatg actgggatct ttttagacca aaaagaagta cggggatcat tagcagaagg
     3181 tttcgcttct gggaaaacag ttttgaacat gtttagttat accggcgcct tttctgttgc
     3241 tgccgcaatg ggaggggcaa tcgaaacaac aagtgtcgat ctagcaaaac gcagtttaaa
     3301 aaaaacacag gaacagtttg aagtgaatgg tttggatgtc gatcagcaaa aaatcattgt
     3361 tatggacgta ttcgagtatt tcaaatacgc caaaagaaaa caatttttat atgacatgat
     3421 tgtattggat ccaccaagtt ttgccagaaa taagaaaaaa gtctttcgcg tagcaaaaga
     3481 ttatggggaa ctggtcaagg atagtctgga tattttagca gaagacggga tacttattgc
     3541 gtctacaaat gctgcgaatg tctcgataga acaattccaa acaatgattg aagaggaatt
     3601 ccatgccaaa aatgtttctt ttgaacaact tgctttatat cgtctgccaa aagatttcca
     3661 gacgacggat acctttttag aaggcaatta tctgaaagtg tttgtttatc aaattaaaaa
     3721 taaccattag aaggagcgat gcagaatggt tcgggtaaag ggattgaata taggaagcgg
     3781 tagaccaaag atttgtgcgc ctcttatcgg ggccgtccga gaggaagtat tacgtgaagc
     3841 agttcttgca cgtgaagcag gggccgattt agtcgaatgg cgagtcgatc attatcgtga
     3901 agttttttct tttgatgaag tagcagaaac attggttctt atccatgaga tgcttgatgg
     3961 actgccgtta ttatttacgt ttcgtacctt agctgaaggc ggagaacaag acatatcgat
     4021 ccgtcagtat cgcgaccttt atgaacatgt gatacacaca ggattagtag atatggtcga
     4081 tatcgagttg ttcaaagtag aaagtctagg aaaaagactg atagaggaaa tcaagtcttt
     4141 aaacattcct ttaattatca gtagtcatga ttttaaagaa acaccagcag accctgtctt
     4201 gctttaccga ttgaatgtta tggaacattt tggagccgat atcggaaaac tggcagtaac
     4261 tcccaataat gaacgagatg ttttgcggtt gatggagttg actagacggg caaatgcttt
     4321 tttctccatg cctattatta cgatgtccat gggaaatctt ggtaaaattt ctcgtctggt
     4381 tggcggaacg acgggttccg ttatgacatt tggttcctta agcagcgatt ctacttctgc
     4441 tccaggacaa ttaccggtca aagcattgaa gcaaattatt caattattaa atgaaccgtt
     4501 gtagaacaag tttgaggtga tattttttgg caaagaaaaa aatagtaaaa acgaatgcaa
     4561 tccgtatggt cgaacaaaaa aagattccct atacagagca cgaatatgag tgggacgaaa
     4621 gccacctaag tgcgtcatcg gtcgctgaac agcttccaga aagccaatct cgtatattta
     4681 aaacactggt tgctgtgggc aatgtgacgg ggcctcttgt tgctgtgata ccgggagaag
     4741 ccgaattgaa tctaaaaaaa ttagcaaaag tttcagggaa taaaaaagta gaaatgctcc
     4801 atttaaaaga tttggaagca acgaccggat acatacgtgg tggctgctcg cctataggta
     4861 tgaaaaaact atttcctact tatttagatc aaattgcaga aagctatgaa caaatcattg
     4921 tctcggctgg cagaagaggg ctacagatgg agttagcacc tcaagatatc tgtgcattaa
     4981 cgagcggaca atttgcagat ataaaacaat aaatcatacg tataaaagaa agaaggaggc
     5041 cgggatgttg tttgtccagt ctccttcttt cttttttata cattatgctc tttgcccggc
     5101 attcattcct gctacatgcc ctgtagcgaa agccgctgtg atattaaagc cgccagtata
     5161 accattgaca tccagtaatt ctcccgcaaa atacagacca tcaatgattt tactctccaa
     5221 tgttttagga taaatttcct ttagagaaac ccctccgcca gttacaaatg atttctcaat
     5281 aggaaaagtt ttgttgattg atagcttaaa ctctttgcat agttgaacaa aatcttgaat
     5341 ttgtttttga gaggcttgtt gtcctgtcag atgatccatt tcaagccgct caagaaagaa
     5401 aactaacaat cgctcaggca atataccatg ccaagcattg accaagttct ttttggttgc
     5461 tttacttttt tctgtcaata tatgaataag ctcttgtttg gtttgtgtag ggaaacaatc
     5521 cagagaaagt gtcactggtt ctcccgtttt ctctaattct ttattgacaa aacttgaaca
     5581 acgcagtgca gccggaccag aaataccgaa atgggtgaaa agtaaatcca tcgtatgttc
     5641 agtcaataca cgaccttttt tatttaaaac gcgtaaagtg atgtcttgta aagaaagccc
     5701 ttgaagtgtt ttctcctgaa tatacggttc atccgatatc aagggggact ctgtcgcata
     5761 aagaggcgtc accgtgtgac ccactcgctt cacgatcttg tacccatctc cagtagaacc
     5821 agtagatgga taagtccgtc caccagttgt taagataatg gcaggagcat ggaactcttc
     5881 gaattccgtg cgtataccgt aaattttatg ttctttgtgg atcaatttct ctactcgagt
     5941 agagaataga agcgtcacat ctagttcttt cagacgattg agcaatgctt cgataatcgt
     6001 ttttgattta tttgttacag gaaacatccg tccatgatcc tcttctttta aatgtacgcc
     6061 ttgtgattca aagaagttca tgatatcgaa attattccat tgcgcaaacg tactatataa
     6121 aaactttcca tttccaggaa tatgtgcaat caaatcatca acaggacgat tgttcgtaac
     6181 attgcaccga ccaccacctg tcatcagtag tttttttcct gcttttttgt ttttttcgat
     6241 caagagtacg cgtgcgcctt gttcagctgc actgatcgct gccatcatcc cgcttgttcc
     6301 agcgccgact acgatcacat catacagttt ttccattccc tcacctcaag gaaagtgtag
     6361 catatctgaa gaaagaaaac agaaaaaaat gttttgagcc aaaagagaaa atgagctaaa
     6421 atgagggaag ataagcaaag gagcgtggaa cgatgacaaa cgcaacagaa tatgttcaag
     6481 caattcaaga aaagctacat caaaaggatc aagggcaaga agaattttta caagcaatcg
     6541 atgaattcat gcccacggtt atgtcttttt tggacactca tcccgaatat atcgaaaaga
     6601 atatcttaga acttttaacg gaaccagaaa gagttattca atttcgagtg ccttggcaag
     6661 atgatgaagg aaactggcga gtaaatagag ggtaccgtat ccaatacaac tcagctatcg
     6721 gcccttataa aggtggattg cgcttccatc ctagtgtcaa tctaagtatc ttgaaatttt
     6781 tggcttttga acagattttc aagaatagtt taaccggtct gccaatcggt ggaggaaaag
     6841 gcggaagtga cttcgatcct aaaggaaaat ctgataatga gatcatgcgt ttttgtcaaa
     6901 gtttcatgct ggaattgtca aaacatatcg gcccatcgat tgatgttcct gcgggagata
     6961 tcggtgtagg agctagagaa atcggctatt tgttcggcga atataaacga ttgaaaaaat
     7021 atgatactgg tgtgttgaca ggtaaacctt tggatttttg gggaagtaag atacgtacgg
     7081 aagctactgg ttatggatta gtgtattatg tcaaacatct attgaacgaa gaaaaagatt
     7141 catttaatca aaaaacagtt tttgtttccg gaagcgggaa tgtagccatt tatgcaatcg
     7201 aaaaagtgca agagctaggc gggaaagttg ttacatgttc agattctagc gggtacatct
     7261 atgatccaga aggaatagat gtgaaactat tgaaagaagt caaagaaatc aatagacaac
     7321 gtttgacgga atacgcaaac caacgtcctt ctgctattta tcatgctgga gaatctgttt
     7381 ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc tactcaaaat gaaatagacg
     7441 aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat cgtagcagaa ggcgctaata
     7501 tgccatgtac agtaaaagct gtttctgttc ttcatgaagc aggcgtgtgg tattgtccag
     7561 gaaaagctgc taatgctgga ggagtagcag tatctgcttt agaaatgagt cagaacgtcc
     7621 agcgattgac ttgggaaaga gagcaagtgg atgagcagct ggatcaaatc atggaaacga
     7681 tttaccatac gtgtcgccaa acagcagccg agtacggcga cgaaaaaaat ctgcttcttg
     7741 gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat ggcagcagaa ggtttagttt
     7801 gaaaaatgaa actttcttgg aaatattgtc agcattttgt ttatttgctt gaaaaataat
     7861 aaaaaaaaac gtaaagtatt acctgtagag taaacctata aggaggagaa caaatggcac
     7921 acattcattt tgactattca aaagtagcac cgtttgtcaa cgaacaagag ttagggtaca
     7981 tgcaaagcca agtaacagca gcccacaacg aattacggga aggtactggc gcaggaagcg
     8041 acttccgcgg ctggatcgat ttaccaacga actatgacaa agaagaattt gcacgtatca
     8101 aagaagcagc taaaaaaatc caatctgatt ctgaagtatt agttgttatc ggtatcggtg
     8161 gttcttattt aggcgctcgt gcagcgatcg acttcttgaa tcacacattc ttcaatttat
     8221 tagatagcaa cacaagaaaa gcaccacaaa tcttctttgc cggaaattca atcagctcaa
     8281 catatattgc tgacttgatc gaagtaatcg gtgatcgcga cttttcagta aacgttattt
     8341 caaaatctgg aacaactact gaaccagcga ttgcgttccg tgtattcaaa gagcttcttg
     8401 ttaagaaata tggacaagaa gaagcaaaca aacgtatcta cgctacaaca gataaagcaa
     8461 aaggtgcagt taaagtagaa gcagatgcag aaggttggga aactttcgtg attcctgatg
     8521 acatcggcgg acgtttctct gtattaactg ctgttggttt gttaccaatc gctgttagtg
     8581 gtgcagatat cgatgcattg atgcaaggtg ccgctgatgc aagcaaagca tactcaagcg
     8641 acaaactaga agaaaacgaa gcttaccaat acgcagcaat gcgcaacatt ctttaccgta
     8701 aaggcaaagt aactgagttg ttgatcaact atgaaccagg aatgcaatac ttctcagaat
     8761 ggtggaaaca attgtatggt gaatctgaag gaaaagacca aaaaggcatc tatccttcaa
     8821 gcgcaaactt ctcaactgat ttgcactctt taggacaatt catccaagaa ggacgtcgca
     8881 atatttttga aacagttgtg aaggttgaaa aacctcgtaa atcaatcaca attcctgaac
     8941 aagcagaaga tttagacggt ctaggctact tgcaaggaaa agaagtggac ttcgtaaata
     9001 caaaagcatt tgaaggaaca ttgcttgctc atacagacgg tgacgtgcct aacttgctag
     9061 tcaaaattcc agaaatggat gcttatacat tgggttatac aatgtacttc tttgaaattg
     9121 ctgtaggtat ttcaggttac ttgaatggaa tcaacccatt cgaccaacca ggtgtagaag
     9181 cttacaagaa aaacatgttc gcacttctag gcaaaccagg ctttgaagat cttgctaaag
     9241 aattgaacga acgcctgtaa gataagggtt tttcgctaga tttaaataat cagtgataga
     9301 aacatgttcg ctttaaaatg aaaactggta cccatttagg tactcaaaca tactgcatcg
     9361 tttattcaga ataaggggct gtgacaagac gtttgtcaca gtcccttgtt tgtatagaaa
     9421 tcgattgatt gccttgctct ttagtttgtt ttaggacttt aagaacagtg aggctgactt
     9481 cgaaggttaa ttctgttttt ttgttttcta gaatcagtaa gcaaaaaagc actcacttcg
     9541 agtgcttttt tgcgatatat tcttagctta agataggcct aatttttctt ttacagatga
     9601 gggtacctta tcgctggtaa ttactttttt ctctatgatc cgcttttcgc ttgctttcaa
     9661 ctgcaagatc gttccttcgg ggtaagcaga accattggca gagtcaccag aagtaatgac
     9721 atcgaaatct aactgtcttt tttcaccgtt ttcagcataa gcatctacgt tgtatttaaa
     9781 acctcttcca attacttccc cattatctgc tttgatatca gtttctgcag gcattggagc
     9841 tttgactacc gcataataat ctttacctac ataagtagac tgatagtagt cccaaccttt
     9901 ccatcctata aaagcaatca atatgataac gatcaaccct aaaatttttt tcatgaaaga
     9961 tctccttttt tattttta
//
LOCUS       DACEMO010000099         9871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEMO010000099 DACEMO010000000
VERSION     DACEMO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.101"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(201..1049)
                     /locus_tag="IW728_001776"
     CDS             complement(201..1049)
                     /locus_tag="IW728_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1025133.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1213..2115
                     /gene="gnd"
                     /locus_tag="IW728_001777"
     CDS             1213..2115
                     /gene="gnd"
                     /locus_tag="IW728_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1025134.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2117..3118
                     /locus_tag="IW728_001778"
     CDS             2117..3118
                     /locus_tag="IW728_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1025135.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3115..3399
                     /locus_tag="IW728_001779"
     CDS             3115..3399
                     /locus_tag="IW728_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1025136.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3432..4229
                     /locus_tag="IW728_001780"
     CDS             3432..4229
                     /locus_tag="IW728_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1025137.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4243..5076
                     /locus_tag="IW728_001781"
     CDS             4243..5076
                     /locus_tag="IW728_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1025138.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5094..5951
                     /locus_tag="IW728_001782"
     CDS             5094..5951
                     /locus_tag="IW728_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1025139.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5973..6481
                     /locus_tag="IW728_001783"
                     /pseudo
     CDS             5973..6481
                     /locus_tag="IW728_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            6518..6919
                     /locus_tag="IW728_001784"
     CDS             6518..6919
                     /locus_tag="IW728_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025140.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7031..7384
                     /locus_tag="IW728_001785"
     CDS             7031..7384
                     /locus_tag="IW728_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025141.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7418..7861
                     /locus_tag="IW728_001786"
     CDS             7418..7861
                     /locus_tag="IW728_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1025142.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7878..8963
                     /gene="uxuA"
                     /locus_tag="IW728_001787"
     CDS             7878..8963
                     /gene="uxuA"
                     /locus_tag="IW728_001787"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1025143.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8956..9669
                     /locus_tag="IW728_001788"
     CDS             8956..9669
                     /locus_tag="IW728_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1025144.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3280 a         1564 c         2182 g         2845 t
ORIGIN      
        1 gtatactaac tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct
       61 ccaaagatgc gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg
      121 cagaaccaaa aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt
      181 tcgctttttc tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg
      241 atagaacgac gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa
      301 atgcttagtt gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa
      361 atagaaataa aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata
      421 acgattgttt ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat
      481 tgccctgtgt gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc
      541 aattgaatat ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga
      601 taagcgtcag ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat
      661 tcattgataa tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca
      721 tttaaagttg aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt
      781 ttctgaatat tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc
      841 attttaaaat ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag
      901 tcagcaatgc ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga
      961 ttttctaaaa tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt
     1021 ttgatttgta gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc
     1081 attttttccg ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa
     1141 aatatttttg taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat
     1201 ggaggcgcta tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct
     1261 ttaaacttaa aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt
     1321 agtcaggcaa gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact
     1381 tttgagcaga aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg
     1441 ttaagaaaac tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct
     1501 tattataaag attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg
     1561 gattgtggga cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga
     1621 ggagataaag aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga
     1681 ggatatctgt atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga
     1741 atcgaatatg gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag
     1801 tatgcttttg acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc
     1861 tggctgatgg aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg
     1921 ggaatcgttt ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct
     1981 gttccagttc cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat
     2041 agtttttcag caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt
     2101 gaaaagggtg agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg
     2161 aagagtcttt ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac
     2221 tgttgatcaa gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat
     2281 tattgcaagt gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct
     2341 attgatttcc cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca
     2401 ccagacaatc gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaataa
     2461 tataactaat ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga
     2521 cgatcgatgg gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg
     2581 tgtgattggt gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg
     2641 ctgtgatgtt ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc
     2701 aaaaaaagta gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa
     2761 tttgacagaa gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa
     2821 tgtttatcta tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg
     2881 tctgaaaagc ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg
     2941 tcaagatcat ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta
     3001 cacaagggaa tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag
     3061 tcagcacctt cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg
     3121 aagaaaatgg tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa
     3181 ggtggtattt caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc
     3241 acccacggaa cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa
     3301 acgattcaat ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga
     3361 atagctacaa tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac
     3421 tgaccgatct agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta
     3481 ctttagctag taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac
     3541 aagcagaagg gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc
     3601 aattagtgat tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa
     3661 aatggtatga atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga
     3721 agctagcaca agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag
     3781 caatccaaga tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta
     3841 tctttgctgt tgctggttta gttggaggat taggagataa atacgcaaga aatgtggcag
     3901 cacatgctat gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg
     3961 gagaaaaagt agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa
     4021 tagacgagtt atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg
     4081 gattatttcc gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag
     4141 agaaagtgca tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg
     4201 aactggaagg ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta
     4261 gtacaaagtt tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga
     4321 ggagccacgt tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc
     4381 atgggggaag ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg
     4441 gttttttctc caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta
     4501 ccagtcgcat tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc
     4561 ggattgctcg gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa
     4621 tatacagaca aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt
     4681 tatccaacgg ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta
     4741 ggggcgccat tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt
     4801 cttgaagtag taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc
     4861 ggtcgaaaag attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa
     4921 tctttagata ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt
     4981 gtccagtcgc aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct
     5041 tttgatgacg aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg
     5101 aaataatgga taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag
     5161 cctatctgag atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc
     5221 catcactact atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt
     5281 taaaagcagc atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg
     5341 gtgtgattac tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag
     5401 aaaaagagat cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg
     5461 cactagcagg aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa
     5521 tcgcggtacc ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct
     5581 ttgggttgta tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc
     5641 gtaatgctac aggcctaaag caaaacactg gtgttcagac agcgattgaa atgctatcag
     5701 ttttagggtt gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat
     5761 tgaaatttgc gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc
     5821 cagggatatt acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat
     5881 tgaaagtcac acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg
     5941 gtattttata aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg
     6001 atgagcgttt gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt
     6061 ccatttttgt tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa
     6121 atgcaggaag ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct
     6181 attgggatga atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtcaaac
     6241 gatttacgac ttagtaaaac acggaatccc catggaagaa ttgaatattg gagggattgc
     6301 tcaaaaaaat cctgatacag atcttttagt gacgaaatct gtttatttaa ataaatcaga
     6361 tgctgaaaaa ttgaaagaaa tgaatgaaca atatggcgta aagaatattt attttcaagc
     6421 cactccatct gcacctaaaa caggattaaa agatgtattg aacaagtttg atttgtcata
     6481 aacaaatgct tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg
     6541 tgattgggaa tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca
     6601 gcgttttctt tcagcattag tatcagatat ttacagtatg agaacagatg tgacagtcat
     6661 tgcttacgat acgctagcct accgatcaaa aaatatttat gttggagata tcgaaaaagc
     6721 agagaaagtt atctgtactt attacgatac acccgttcat gcattaggaa gttatttcat
     6781 gtttgattgg aaagaccaaa ggaaaaaaac aatctactct atattattgt ccttcatttt
     6841 gttgttcagt ttaggttggt ggggaatgat gatatataac aaaaatcctc atcacgtatt
     6901 cgatctgcta tccgtataaa caggtatcac tgtactagca tttgggagct actttttctt
     6961 attgggaaaa gcagctcgcg gatggcctag cagacaaact tttattcgaa atacttcatc
     7021 catattaacg atgctagaaa tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt
     7081 tgttgatgaa ggctgttatg gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa
     7141 agaggctatt cttttttatc tagactctgt tggggcagat acaccgctac agttttcggg
     7201 aaattatttt tcaaataaag aacagtggct gaaacaagtt gataagttga aagaaaaaaa
     7261 cgtcaattat atttttagtg caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac
     7321 agatttacga gggaaaacat ttaattggca aaatgccaat cagattattg ctctttttcg
     7381 ataagtaaaa agtgcaaaac aaggagtgag aagactaatg ttggaaatca tgatcgttac
     7441 acatggtaag ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag
     7501 ccatattgaa acaatgtgct tagagtctgg ggaggacatc caaacattcg gagagaaggt
     7561 caaagaaaaa ttaactatga tcaacacagc agaacccatg ctagtatttg ttgatctaat
     7621 tagcgcaagt ccgtacaatc aagcgctctt ggcagtcaat gaactgactg aagaaaagaa
     7681 agcagccact tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag caatcaatca
     7741 tcagatgctt cacacaccga tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga
     7801 taatttaact gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta
     7861 gaaggaaggc tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga
     7921 tcccattaaa atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga
     7981 ctaaaatgcc tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg
     8041 agagccaagg actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg
     8101 cagggacaga tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt
     8161 ctcgatgtgg ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa
     8221 ctgatttgtt taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg
     8281 tcgatcagat ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca
     8341 aattgccggg ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg
     8401 aaggaatgac cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc
     8461 cagtgtgcga aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac
     8521 tttttggttt tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag
     8581 tagattctcc atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa
     8641 acgatttagt tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa
     8701 acgtggagtt tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct
     8761 cccttgatat gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac
     8821 gccctgatca tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg
     8881 atcgtgcaat gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac
     8941 cactgggaga gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta
     9001 ttagaaactg gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat
     9061 gaaatgcgta aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa
     9121 caaatgggca ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat
     9181 caatataaag aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa
     9241 gaaattttat cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca
     9301 cgggtaaaag tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc
     9361 atttcagggg atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt
     9421 tcaaaaatga atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc
     9481 gaagcagcaa ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg
     9541 cccgatcata atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa
     9601 gcagactatg aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg
     9661 tatctctaat aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag
     9721 ggaacagatg aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac
     9781 aatttagaac aaacacaaaa cgcatctact gttatctacc agtagatgcg tttaaagaga
     9841 agaacgagaa tcgtccatat gagggtttcc a
//
LOCUS       DACEMO010000100         9674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEMO010000100 DACEMO010000000
VERSION     DACEMO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.103"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(22..2256)
                     /locus_tag="IW728_001789"
     CDS             complement(22..2256)
                     /locus_tag="IW728_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1025145.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            2735..3001
                     /locus_tag="IW728_001790"
     CDS             2735..3001
                     /locus_tag="IW728_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1025146.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3001..4728
                     /gene="ptsP"
                     /locus_tag="IW728_001791"
     CDS             3001..4728
                     /gene="ptsP"
                     /locus_tag="IW728_001791"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1025147.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            4859..5938
                     /locus_tag="IW728_001792"
     CDS             4859..5938
                     /locus_tag="IW728_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1025148.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            5955..7178
                     /locus_tag="IW728_001793"
     CDS             5955..7178
                     /locus_tag="IW728_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1025149.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7267..8154
                     /locus_tag="IW728_001794"
     CDS             7267..8154
                     /locus_tag="IW728_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1025150.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8240..8470
                     /locus_tag="IW728_001795"
     CDS             8240..8470
                     /locus_tag="IW728_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1025151.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(8527..9492)
                     /locus_tag="IW728_001796"
     CDS             complement(8527..9492)
                     /locus_tag="IW728_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1025152.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3117 a         1706 c         1960 g         2891 t
ORIGIN      
        1 tctcacgcta taaaactgga tttattcagc cgtttcttcg gctgattctt tagccaaacg
       61 ttctggttta gaagtaacga tgatttttcc atctttatcc agttttgctt tcaactcatg
      121 gatagaaggg tgatcaagat aaaattcagc gataccatct tcgatttgtt cttggatcgt
      181 gcgtcgaaga ggacgagcac ccatagatgg atcatagcct aagtcaacta atttttcttt
      241 gacattcgta ggaacttcga tgtgaagttg ctgtgctgcc agcatttggt tgacatcgtc
      301 tagcattagt gtgacgattg tcatcaagtt ttcttttgat aatgcactga attcgatgat
      361 cccgtcaaaa cggttcagaa attctggtgt aaagtagttg tttaattggt tcaatacaga
      421 acgagtaaca ccttcgcgtg ccgcaccaaa accaacgttt gcttctactt taccagttcc
      481 ggcattactt gtcataatga tgatcgtatc tttaaagctg accgttcttc cttgtgcatc
      541 tgtcaatcga ccatcatcta agatttgcaa gaacatatgc aaaacatctg gatgagcttt
      601 ttctacttca tctaacaaga caagactgta aggatttctc cgtacttttt ctgttaattg
      661 accagcttct tcgtatccga cataacctgg aggagaacca atcaatttag agacactatg
      721 tttttccata tattcagaca tatcgaaacg aatcattgaa tcttgtgaac cgaatagttc
      781 gtaggcaagt tgtttcgcaa gctctgtttt acctacacca gttggtccaa caaataagaa
      841 ggagccgatt ggacgattct gtttgtttaa tccaacacgg tttcggcgaa tcgctttcgc
      901 tactcgatct acagcgttat cttgtccgat gacatgagct ttcaagtcat ctgctaagtt
      961 tttcagttgt gtctgttctt tttcttttaa ttcacccact ggaatacctg tccgttgttc
     1021 aacgattttt tccatatctt tttcggtaat cacaggtgtt tcctcttcag tgagctgacg
     1081 ttctttcatt ttttgaagtt tattgatctg atcgcgataa taagctgctt tttcgaaatc
     1141 ttcttctcga gaagctaaaa ctttttgttc ttctgcttct tgcagttttt tctcgattgt
     1201 tttaggatca acgatttgga tcgtcaagtt tttcttagaa cctgtttcat ctagcaaatc
     1261 gatggcttta tccggcaaga agcgatcttg gacgtaacga ttggataaag ttgcggctgc
     1321 ttcgatggcc tcatcagtat attttacatg atgatagtct tcataacgtt tttgcaaacc
     1381 tttcaaaata ctgatcgttt cttcgacagt tggttcatct acccgtacag gttgcatacg
     1441 acgttctaaa gcagcgtctt tttcgatgat gcggtactca tttaaggtag tagcaccaac
     1501 catttgcaat tctcctcgag ctaaagctgg ttttaaaata ttaccagcat ccatatttcc
     1561 atcaccagca gaaccagcac cgactatttc gtgaacttca tctatgaata ggatcacatt
     1621 ttctgcttga cggatctctt caatcaattt ttgcatacgt tcttcaaatt gtcctcgtat
     1681 accagtacct tggactaaag atacgacatc taaacgaatg acttctttgt ccattaattt
     1741 ttgcggtaca tcaccatcta cgattttttg tgctaaacct tcaacaactg ctgttttacc
     1801 aacacctggt tctccaatca agacaggatt gttcttggtt cggcgattta aaatttcaat
     1861 gactcgtttg atttcgtcat cgcgtccgat cacaggatcg atatcacctt gtcgtgcttg
     1921 ttctgtgata ttgattccgt attctcccaa taaaccttct gtattgcctt gaggtggctg
     1981 accaccattg aagttgttgt ttccgccaaa ttgtgttggt ggtgtttgct cataatttcc
     2041 ttgctgttgc atttggcgag acatggaacg gaaaagatcg tccaaactgc caaatccaaa
     2101 tggatcattt tgggccatat tagtcagacc tccattttgt tgatttttga ttttttgata
     2161 acagctctga cagtaatcga gttgtgtccg ctgaccattg acagtggcat acaagtgaat
     2221 cgttgcctca ttcttgccgc agttttgaca aagcatgaat cattcacgtc ctttcacaaa
     2281 aatgggtaca tccatatttt acatctcgtt tttcatgagg taaaagaata acccctagtt
     2341 ttttaagaga tttatgaggt agaaaaggtg aaatctctat tgaccattcc tgacctttga
     2401 ctcttattat actaaaaata aaaagggaag caagtaattc gtttcatatg aaaacaaaga
     2461 gggtcaaagg aaagaatgat aaacgtcagg agaagagaaa gcgacaagaa aaaggaaata
     2521 aacacgtttg ttataaaatg tgaagaaaag gatgaaaaag aatccggaat aatttgaata
     2581 cttttatctg aaattgaatg aaaactgttg tatctaacga aaaaaaatgt taatctttac
     2641 agatgaaagg gtggcatttg ttaggattct ctgaattact aaaaaagaaa accctcacat
     2701 aaaaacaaca ctcacttaaa ggagaccgat taatatggaa aagaaagaat ttcacgtagt
     2761 agcagaaact gggatccacg ctcgtccagc tacactatta gtacaaacag caagcaaatt
     2821 taactctgat gtaaacttag agtataaagg taaatcagta aacttgaaat caatcatggg
     2881 cgttatgtct ttaggtgtag gtcaaggttc tgatgttact attactgcag aaggtgctga
     2941 cgaagctgat gcaatggcag caatcgttga aacaatgaag aaagaaggct tatctgaata
     3001 atggttgaaa tgctaaaagg gattgccgct agtgacggtg tagccgttgc aaaagcttac
     3061 ctgctagttc aaccggattt aacattcagt aaggcaacag tagaagatac tgcggctgaa
     3121 gaagctcgtt tggatgctgc attagctaaa tctactgaag aattgcagca aattcgtgaa
     3181 aaagcagcgc aaagcttagg tgaagcagaa gcgcaagtat ttgatgccca tttgatggtt
     3241 ctttcagatc cagaaatggt aggtcaaatc aagcaaaata ttaaagacaa cagcgtaaat
     3301 gctgaatcag cgctaaaaga agtaacggat atgtatatcg gtatgttcga agcaatggaa
     3361 gataatgctt atatgcaaga gcgtgctgca gatattcgtg acgttgctaa acgtatttta
     3421 gcgcacttat taggcgttac tttaccaaat ccatcaatga tcaacgaaga agtggtcgtt
     3481 gttgcacatg acttgactcc aagtgataca gcacaattag atcgcaattt tgtaaaagcg
     3541 tttgtaacag acattggtgg acgtacttca cattctgcta tcatggcacg ttctttagaa
     3601 attccagcaa tcgttgggac aaaagaaatt actgctaaag tcaaagaagg cgatattttg
     3661 gctgtcaacg ggatcgaagg cgatgtaatc atcgatccta cagatgaaca aaaagctgaa
     3721 tttgaaaaag caggtgcaga ttacgcagca caaaaagcag aatgggaaaa attaaaaaat
     3781 gcagaaactg tcacagctga cggcaagcat tttgaattag ctgcaaacat cggaacacca
     3841 aaagatctag ttggtgtcca taataatggt ggagaagcag ttggtctgta ccgtactgaa
     3901 ttcctgtata tggattctcc agacttccca acagaagatg accaatatga agcttacaaa
     3961 gctgtattag aaggtatgga aggaaaacct gtagttgttc gtacaatgga catcggtgga
     4021 gataaagaac taccatatct gcaattacct catgaaatga atccattctt aggatatcgt
     4081 gcattgcgta tcagtttatc tgaacaaggc gacgaaatgt tccgtacaca aatgcgtgca
     4141 ttgcttcgtg catctgttca tggtaattta cgtatcatgt tccctatggt agctacactc
     4201 aaagaattcc gcgcagcaaa agcgatcttt gaagaagaaa aacaaaaatt gataagtgaa
     4261 ggcaaggaag tatctgatac gatccaagtc ggcatcatga tcgagattcc agcagcagca
     4321 gtcttagcag acaagtttgc aaaagaagta gatttcttct ctgtaggtac gaacgatttg
     4381 attcaataca caatggcagc agaccgtatg aacgaacgcg tttcttactt gtaccaacca
     4441 tataacccat caattttacg tttgatcaaa aatgtaattg atgcagcaca cgctgaagga
     4501 aaatgggcag gtatgtgtgg agaaatggct ggagatcaaa cagctgttcc attgctagta
     4561 ggtatgggtc tagatgagtt ctcaatgagt gcgacatcta ttcttaaaac gcgtagttta
     4621 atgaaacgct tggacacagc gaaaatggct gaacttgctg atcgtgcatt gaaagaatgc
     4681 gatacgatgg aagaagtcgt tgaacttgtt catgaatatg taaaataaac aacgtatcaa
     4741 aagctggtgt ggcttgtaaa agagctacgc cagttttttt gttctttttt aagaaaacgt
     4801 ttttgtcagt aagaatagac aaaaataggg cttaagtttg agtgacagtt tgtaggctat
     4861 gttatactat ataagtgaag ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga
     4921 aaagatgctt gcaaaatctg gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac
     4981 ggaagtcggt atctcttata cactagatga aaaagaagat tatgacatcc tgcacatcaa
     5041 tacatacgga atcaatagtc ataatatggt caataaagcc agaagagaag gaaaaaaagt
     5101 tgtctatcat gcgcattcta cagaggaaga tttccgaaat tcctttatcg gatcgaacca
     5161 gctttctcct atcgtaaaaa aatatctggt aggattgtac caaaaagcgg actatctgat
     5221 cacgccaaca ccttattcaa aacagttgtt ggaaagctat ggtatccgtg tgccgattca
     5281 agctatttcc aatgggatcg acttagagaa atatcaccct gatccaatga aagaacaaaa
     5341 gttcagagaa tatttcaaac tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt
     5401 tttcgaacgt aaaggaatca ttgatttcgt agaaattgct aagaagatgc ccgaatacac
     5461 atttatttgg tttggtcatg ttcccatgta ctcgattcct cggaacatcc ggaaaattgt
     5521 aaaagaagat catcctgaaa atgtcctgtt tcctggttac attcgtggag agatcataga
     5581 aggtgcgtat tcaggtgcag atctattctt tttcccttct tatgaagaga cagaagggat
     5641 cgttgtttta gaagctttag caagcaaaca aaacgtactt gttcgagata ttccagtgta
     5701 taatggctgg ttagaagata gcaagaactg ctatatgggg aagacaaacg aagaatttat
     5761 ccgactgatc caacagatta ccaatcaaga actaccgagt acgacacaag ccggttatca
     5821 aacagcaaaa gagcgaagta tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag
     5881 tgtactgaat gaaaaagtct ctagcaaatt aaaaaaagta aatcaggtca aggactagtt
     5941 tggaggcgaa aaacatgaaa atcggctttt ttaccgatac ctactttccg caagtcagcg
     6001 gagttgctac atcgatcaaa acattaaaac aagaattaga aagacatggg catgaagtct
     6061 atatttttac aacgaccgat cctaatgcgg ataagttgga aaaagatgtg attcgaatgc
     6121 caagtgtacc atttatttct ttcaaggatc gaagagttgt tgttagaggc atgtggtatg
     6181 cttatttggt tgccaaagaa ctagaactag atcttattca tacccataca gaattcggag
     6241 cagggatact tgggaagatg gtagctaaga agttgaaaat acctctgatc catacttacc
     6301 atacgatgta tgaagactac cttcattata tcggtaaagg aaaagtgatc aggcaatccc
     6361 atgtaaaata cttgtctaga ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg
     6421 aacgtgttat tgatacatta cgcagctatg gtgtaatagc accgcttcgc gtgattccta
     6481 ctggtatcga cattgaaaaa ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat
     6541 taagggagtc actaggtctt caggaaaacc atttgatgat tctttcttta agcaggattt
     6601 cttatgagaa aaatatccaa gccttgatta atgggtttcc tcaggtcatc tccgcttatc
     6661 ctaacgccag attagttatc gtaggaaaag gaccttatgt agaagagtta gaggaattga
     6721 tctcagaact gcagttagaa gaatttgtgc agttcacagg agaagtggac aacaatgatg
     6781 tagcacttta ttataaagcg gcagattatt ttgtcagtgc ttctacttca gaaacacaag
     6841 ggcttactta tacagaagcg atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt
     6901 atttgaaccg attgttcgat catccttcat tagggaaaac ttttgaaacc gacgaagact
     6961 ttgcaccagc acttattgat tatatttcca gtgaagttcc taaagatcca aaagttttag
     7021 aagctaaact ttatgatatt tcatctacac atttcggcga agctatgatc gaattttatc
     7081 aagacatggt tgtttatcat gaagaagaga tgcgaagaaa agaagaagaa gactcgattg
     7141 aacgaatcaa aataaaatta aattcattta aaagataaaa ttcaaacatt ttttatgaaa
     7201 aaatccttgt ttctagcgta tactagcata gaagcaagga tttttgattt ttaggaggtc
     7261 gaaaaaatga aacttggttt tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat
     7321 ctactagaag caggacacga agtagctgtc tataatcgga caaaagcaaa agctgatcct
     7381 ttagtaacag aaggagctat ctgggcagat acaccaaaag cggtagcaga acaaagtaat
     7441 attctcttta caatggtagg ttaccccaaa gatgtagaag aaatctacta tggacagtcg
     7501 gggatttttt ctgcagatat ctcaggacat attttagttg atttgactac aagtacacca
     7561 agcctggcag aaaaaattgc aaaaacagca aaagaaaaag gcgcagatgc gttagatgca
     7621 ccggtatccg gtggtgactt aggagcaaag aatggtacac tgacgatcat ggtcggtgga
     7681 gaagaagccg tttatgatca agtactgcct ttattcaaag aatttggtac gacatttaca
     7741 ttgcatggtt ctgctgggaa aggtcagcat acaaaaatgg ctaatcagat catgattgca
     7801 ggtacgatga caggaatgac ggaaatgctt gtctatgcac aaaaaaacgg acttgattta
     7861 aagaaagtga ttgagacgct ttcaggtgga agtgcagcta actggtcttt aagcaattat
     7921 tctcctcgta ttttaaaaga agattacaca cctggttttt ttgtcaaaca ttttatcaaa
     7981 gatttaaaga ttgcattaga agaagcagaa aaaatggatc ttgtattgcc agcaactacc
     8041 caagcattga aactatatga agagttggca gataaaggtt ttgagaatga cggaacccaa
     8101 gctttgatca aactttggtg gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg
     8161 agttttttta gagaaaatca atgcaagtag gctatgcttt tgatacaata taacagaaat
     8221 aagaaaagga ggacctccga tgacacattc acaattagtc gcaatcataa aacgactgga
     8281 ggctatggtc gagtcagcag ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt
     8341 agagaagtgt acagtaactt atgataaagc gaccgataca tttgaattaa ctgaaacaga
     8401 tactcatcag caatacgaat ttgataatat cgatatcgta gcgatggaaa tctatgactt
     8461 aatccaataa attttaggat aattgacatt tgaagttgaa gatgcctaaa agaagccgag
     8521 aagcaactaa tttagttgct tttcagcttc ttttttgttt aacagttcaa tttcaaactg
     8581 ttctttttct aattttttgc ggtaactgat caagatttgt acaggatcga tttcttcttt
     8641 tgacaattga cgtttcaaag ataaatgagc ttctttcttt tcataataga agtcaggaga
     8701 aacgtcacca gaggtaatca ttgtttctgc aagttgacca taacaaacga ggtagtgctt
     8761 tttctgactc tcatctgctg ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa
     8821 ctgcttttgt tcctggatcc gtctaataaa ataagtaact aattcgatat ctttttgaag
     8881 cataccggct ttttcgaaat tcaactgata gatgaaatgt tgttgtcttt ttttcaagta
     8941 agtcagtagc tttttatttc ggccttgaaa cagcccttgg agttcctttt ttttctgttc
     9001 aaaagtcatt tcctgtaggt caggaagctg gactcgcaaa gctaacaaac taatttcaga
     9061 caaccaaggc atttgataag tttcttccat tatacgcaaa atagacggca tttttttata
     9121 gagacgaaaa ggcccgtatg ttcgagccgt aggagtatct gttaatacaa atcccttatt
     9181 ggtaatatgt acataattat aattactgaa ataattcatt tgtcggttat aaaaaggacg
     9241 gtaatgttgg atcaattgac actcgagcaa taaagcatct aattccgtgt ctactacgac
     9301 gaaatcaaaa tctgttatat gatgaatcat acgtaatact ttttttgaat gctgtttatt
     9361 ctgatgaaaa tagctgctta ctctattttt gagccgtttt gctttaccaa catagatgac
     9421 cttaccatct cggtctttca taagatagac acccggcagc atggggagag ttttcatttt
     9481 ttcttttaac ataggatctc cttttacgct ttgaataaaa agactataac aaattttgaa
     9541 ataatggaca agttttaggg aattctatag ttctgttcac agaaaatact ttgataatca
     9601 aaatatgtga gatagatgat ttttctaata aaagaaatta taaatgctga tttatcagtt
     9661 gttttaaatg agta
//
LOCUS       DACEMO010000101         9650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEMO010000101 DACEMO010000000
VERSION     DACEMO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.104"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(13..996)
                     /locus_tag="IW728_001797"
     CDS             complement(13..996)
                     /locus_tag="IW728_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1025153.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1001..1648)
                     /locus_tag="IW728_001798"
     CDS             complement(1001..1648)
                     /locus_tag="IW728_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1025154.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1733..2365)
                     /locus_tag="IW728_001799"
     CDS             complement(1733..2365)
                     /locus_tag="IW728_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1025155.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IW728_001800"
     CDS             complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IW728_001800"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1025156.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2929..3666)
                     /locus_tag="IW728_001801"
     CDS             complement(2929..3666)
                     /locus_tag="IW728_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1025157.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3663..4652)
                     /locus_tag="IW728_001802"
     CDS             complement(3663..4652)
                     /locus_tag="IW728_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1025158.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4910..5452
                     /locus_tag="IW728_001803"
     CDS             4910..5452
                     /locus_tag="IW728_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1025159.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5469..6554
                     /locus_tag="IW728_001804"
     CDS             5469..6554
                     /locus_tag="IW728_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025160.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6566..7372
                     /locus_tag="IW728_001805"
     CDS             6566..7372
                     /locus_tag="IW728_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1025161.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7373..8200
                     /locus_tag="IW728_001806"
     CDS             7373..8200
                     /locus_tag="IW728_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1025162.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8197..9270
                     /locus_tag="IW728_001807"
     CDS             8197..9270
                     /locus_tag="IW728_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1025163.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         2925 a         1773 c         1967 g         2985 t
ORIGIN      
        1 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
       61 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
      121 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
      181 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
      241 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
      301 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
      361 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
      421 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
      481 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
      541 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
      601 aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
      661 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
      721 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
      781 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
      841 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
      901 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
      961 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     1021 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     1081 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     1141 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     1201 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     1261 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     1321 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     1381 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     1441 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     1501 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     1561 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     1621 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     1681 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     1741 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     1801 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     1861 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     1921 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     1981 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     2041 ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     2101 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     2161 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     2221 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     2281 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     2341 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     2401 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     2461 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     2521 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     2581 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     2641 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     2701 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     2761 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     2821 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     2881 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     2941 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     3001 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     3061 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     3121 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     3181 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     3241 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     3301 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     3361 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     3421 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     3481 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     3541 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     3601 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     3661 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     3721 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     3781 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     3841 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     3901 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     3961 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     4021 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     4081 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
     4141 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     4201 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     4261 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     4321 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     4381 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     4441 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     4501 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     4561 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     4621 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
     4681 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
     4741 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatattca
     4801 gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact aatacgagtg
     4861 gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta tggaaattgg
     4921 tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac taggcgaacg
     4981 cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt caccatcaat
     5041 ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat tttttcatca
     5101 agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt atttcgatga
     5161 agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag aaatggagcc
     5221 tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt cattatctga
     5281 aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga cggtctatgt
     5341 ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat taaggaatca
     5401 tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt aatatagggg
     5461 gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc tatgatgatg
     5521 aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat acactgcttg
     5581 gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc accgaagcta
     5641 cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct aataagcgac
     5701 aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt tttgataacg
     5761 ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag
     5821 atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt gaaatgtctg
     5881 gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg aaagtattgc
     5941 tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg cagtatgaac
     6001 tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat gaccaagaag
     6061 aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc gtccaaagtg
     6121 gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat tttgttgggg
     6181 agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt gttggtaagc
     6241 aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt gttttacgcc
     6301 cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg gtcgatactc
     6361 agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa aatgaatgga
     6421 tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc tttgagccag
     6481 aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca
     6541 gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt ccgtccctta
     6601 tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt atcaatcttt
     6661 ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta cgtcggggac
     6721 atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt ttttcacgct
     6781 gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc aattatggct
     6841 gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg catttattgg
     6901 gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga tcgggccgaa
     6961 acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg agattccatt
     7021 tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga tcagtgctag
     7081 tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc cgttatcatt
     7141 aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt tattcatgct
     7201 gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg aacagcattt
     7261 cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa tcattgctat
     7321 gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa
     7381 atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg caccgatctt
     7441 ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta caggatttac
     7501 ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg tattgaatac
     7561 gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat ttggggctat
     7621 gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca tgaataacat
     7681 cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct ttacactact
     7741 aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca gcattccaat
     7801 cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg tcgatgcggc
     7861 tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc cttatctttc
     7921 tccaggaatc atagctggat attttatggc atttacctat tctcttgatg attttgcggt
     7981 tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct attctcgtgc
     8041 aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct tgttctccat
     8101 gatattggtc attggatact actttattag taaagaaaac cgtccaaaac ggctgaagaa
     8161 aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca atctctgttt
     8221 ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca
     8281 agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta tatcgatcct
     8341 agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga aacatttgat
     8401 tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga catcgctata
     8461 ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc attagatcat
     8521 tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga ttttgatcca
     8581 gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata taacgataaa
     8641 ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga attaaaagac
     8701 agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa tagtttaggc
     8761 tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa attgaatcat
     8821 ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat gatcaatgaa
     8881 gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg ggaaaatgaa
     8941 catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa tatcgttatg
     9001 ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat gctagaaccg
     9061 gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga agaagcaaaa
     9121 gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga tgacacgatc
     9181 tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta taatgaccta
     9241 ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat ggggacttat
     9301 caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa tcttctacca
     9361 aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac gtttttctac
     9421 cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag taaatctaag
     9481 attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac ttaacccttc
     9541 tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc tatgaaaaaa
     9601 cataaaaatc atattagata aaaactaaat tggtagataa attattttgt
//
LOCUS       DACEMO010000102         9620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEMO010000102 DACEMO010000000
VERSION     DACEMO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.105"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..743)
                     /locus_tag="IW728_001808"
     CDS             complement(81..743)
                     /locus_tag="IW728_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025164.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(756..2258)
                     /locus_tag="IW728_001809"
     CDS             complement(756..2258)
                     /locus_tag="IW728_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1025165.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2806..4485
                     /locus_tag="IW728_001810"
     CDS             2806..4485
                     /locus_tag="IW728_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1025166.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4612..5091
                     /locus_tag="IW728_001811"
     CDS             4612..5091
                     /locus_tag="IW728_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1025167.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5310..5990
                     /locus_tag="IW728_001812"
     CDS             5310..5990
                     /locus_tag="IW728_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1025168.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5998..7329
                     /locus_tag="IW728_001813"
     CDS             5998..7329
                     /locus_tag="IW728_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1025169.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7344..8306
                     /locus_tag="IW728_001814"
     CDS             7344..8306
                     /locus_tag="IW728_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1025170.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8417..8755
                     /locus_tag="IW728_001815"
     CDS             8417..8755
                     /locus_tag="IW728_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1025171.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8834..9541
                     /locus_tag="IW728_001816"
     CDS             8834..9541
                     /locus_tag="IW728_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1025172.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         3060 a         1732 c         2063 g         2765 t
ORIGIN      
        1 aatcaagcaa ataaaaaatc cgaaccatat ctgcgagaca gatatggttc ggatttttta
       61 tttgatcatt atcctgtttt ttaaacttcg attttttgaa attttttatt ttttagttca
      121 aagacttcat cacagctttt tgctacttca atctcatgag tcacgatgat aatacagcga
      181 ttttgttcat gggcgatttc ttggaaaagt ttgacgatat cttgtgaagt tgtttcatct
      241 agatttccag taggctcatc cgctacgatt agttgatgtt cacagcagat agcacgaacg
      301 atagcgactc gctgctgctg acctcccgaa agtttggtca cctgtttttt tgctaatatt
      361 tcatcaattc ctacacgagc cagtcgatct aaagcaactt gtcgataatt ctcttgcttg
      421 gtcttagtga tttccattgc tgctatgacg ttgtctagag ccgataaata aggaagcaag
      481 ttgtaagctt ggaaaataat cgagacatca tttttacgat aacttcttaa cccgattttg
      541 ttcagtgaac gatcttgata gtataccttt ccttcttttg gtgcatctag tccagaaata
      601 agcgaaagaa aagtcgtttt tccagaacca cttgctccta aaatagcgta catttttcca
      661 gcttcgaaat tcagttctac atctttataa agcgcatctt gcggatcttg ataccaaaat
      721 cccaattgtt ctgtccgtaa catcaaattc ctcctttagt ttaataggat ttttcttggg
      781 tttaatctta atatccctat agaagctaaa aagacagaaa acagactgat tccaaatgct
      841 actccggcta atagtcccaa ttgtgcaggt ttgacattga tttccaattc gctgacttga
      901 tcagaaacag caaatggatt attcgtggtt ccttgttgac catcgcgttg accgccttgt
      961 tcttgtctgt tacctggcat ttgtccaccg ccaggagctt gctggttttg tgtcgtggtt
     1021 tctgtctgtt ggtcaagtag ctgctgtccc acggcattcg cgacgacgtt tccgctaagc
     1081 gaagcaactc ctaatgcaag aatcatacaa atagccactt cggtaaacaa ttggagaatg
     1141 attttacttc tggattctcc taaagatagc aagacgccta actcatgccg acgctcccga
     1201 atggtaatca taatgatcag agtaagtatg ataataccgg ctacagccac tagtaaaaca
     1261 acatttttag aaaaacttgc cacgttgttt aaaggttcca acattgactg atacatggaa
     1321 tcatttgatt gtaagctaaa tgtatctgtg tcgatcagct tctcagcttc tttgagaaac
     1381 tcatccattt tatctggatc agatagagtg taaatagcag aatctatcgt gttatcgtca
     1441 gatgtcccat ttagttcatt tgcaaaagtg taagaagtgt aaagtgtatt cgacggattc
     1501 atgaagttga atttcatacc catactgccg cttgtttcgc tggattcata aattcctttg
     1561 atcgtcattt cataagtggt atcttcatct tcagtactcg tgattgtgaa agtatctccg
     1621 actgccaggt catttgtttc tgctagagta gaatcaatga ctacattgtt tgtgcctttg
     1681 tcgctgtcat ctattccttc accatccgtg atttttgctg ttccatcaga aaaattacta
     1741 ttttgtgcag ttgctgacac accactgatt tgaaagtctg cttgagtcat ttgcggattc
     1801 ccgcccattc ctccagcagg ttcaccgcct gcatcagtag tttctgaact gtcttcactt
     1861 gaatcagaac tagtgatggc agttattcca tcttttgcca tagctgaggc ggaactttca
     1921 aaggaataac tctttacatt atctaattca gctatttttt ccgcgtccgt taaggaaact
     1981 ggtgtaagag aaaaactgcc tggatctgga cgttcttcag tggtagaacc gcttgaagaa
     2041 gtttgttcct gttttttaaa tgaagcttct cgattggcac ttaaagtcac tgttgctcca
     2101 acttcttttt gtgcttgatt cgctgcttct tctgcagcgc ttcgaatcgt gaggccagct
     2161 agcacaaaaa taagaatggc tgtgaacacg gcaaacaata acaatgaacg tccccatttt
     2221 acttttgtac ttttccatgc acgtttcata aaattcatat tttgcatccc tcctagtaga
     2281 tgcatttgat aatagacaac ctatcttaaa gctagcttaa atcttatgct gtttgcacac
     2341 catcaacatg aggttctgta tatataaaaa gtcactatag ttattgcaaa aaatgtggta
     2401 aaatgaaaaa agtatgtttc aaaccgacga agaagaaatg aaagaggaat agcaaaagaa
     2461 atcctagatg ctacagaggg atttttacgt gcatagttgt tctaacaatt gctgatacta
     2521 gcgatttagt ttatttgttg tataataatg gggaacagct gaatacttat ataagtattc
     2581 tggctatagc gtttcgcaaa agatccattt tgttaatgaa gtatatccaa aataaaatgc
     2641 ccatccaact gtcagaacgc ggatgtgctg gtaattttcc tgctgattct ccataagttg
     2701 agaaatagtg attttgcata tgcttcattg gaatggctca tatatttaga taaagaagaa
     2761 aatcgacatc tttaaacttg gatgtcataa gaagaggtga agtttgtgag taccattaaa
     2821 attatcccat taggcggcgt tcgtgaaaat ggaaaaaaca tgtatgtcgc agaagtgggc
     2881 gaggatattt ttattttaga ttgtgggcta aaatatcctg aaaatgagtt gttggggatc
     2941 gacgtcgtaa tccctgattt tacgtattta gaagaaaaca ttgatcgtgt agctggtgtc
     3001 tttttaacac atggacatgc agatgcgatc ggggcacttc cttacttgtt gtcaaaaatt
     3061 tctgtaccag tattcgggac agagttgacg atcgagttag caaaattgaa tgtcagtcgt
     3121 aatgaagcag ccaaaggatt taaggatttc catgtggtag atgagcatac agaaatagac
     3181 tttggtgaaa cagtagtgag ctttttccgt accactcata cgataccaga ttcagtgggg
     3241 atcaatctga aaacaaaaga aggtagtatc gtttatacgg gagattttaa atttgaccaa
     3301 acggctatcc caatgtatca aacagattat gcacgattgg cggaaatcgg caaagaagga
     3361 gtcttagcct tactaagtga atcggcaaat gcagaaaatc ctcagcctgt agcttctgaa
     3421 gcccaaatag cagatgaagt atttgatacg atcaaatatt gggaagggcg tatcatcgtc
     3481 gcttgtgtag caagtaattt gcaacgagtg caacaagtat tggatgcagc agaaaaagca
     3541 caacgaaaag ttgtcttgac tggtcaagat ttcgaacgaa ttattcgcac agcgatgcgt
     3601 ttagaaaaac ttcaattacc aagtgaagat ctgcttgtta cgttgaaaga catgaaaaac
     3661 tatgatccag aagaactgat cattttagaa actggccgaa tgggtgaacc catcaagtct
     3721 ctgcaaaaaa tggcaaacgg tacacaccgt aatgtcaaga ttgaagaagg agatttggtt
     3781 tatatcacta ctacgccaag catcgcgatg gaaaccatcg tggcgaaaac ggaagatatc
     3841 atttatcgag ctggtggaac agtgaagttg atctctgaga atatgcgcgt ttctggccat
     3901 gccaatccta atgacttgca attaatgatc aatttgatga aaccaacata ttttgtgcca
     3961 gttcaaggag agtatcgtga attagctgct catgcagatt tggctcatgc agtagggatg
     4021 ccatacaaaa atatctatat tactggacgc ggagacgtgc tggaatataa gaacaagcgt
     4081 atgagtgttg caggaactac aagtgcagaa aatgtaatga ttgatggatt aggcataggt
     4141 gatatcggaa acattgtttt acgtgatcga aaaatcttgt cagaagacgg gattttcgta
     4201 gcggttgtca ctatcagtcg gcgagaaaag aaaatcattt caaaacctca aattacttca
     4261 cggggattcg tttacgtaaa agctagccgt gatctgatga aagaaagcag caatatgatt
     4321 gaaacaatcg ttgagaaaca tttagaaagc aatgattttg agtggagcaa attaaagcaa
     4381 gacatccgag atcaattagg ccgttactta tttgaacaaa caaaacgccg cccagtgatc
     4441 ttgccagtca ttatggaagc tactcaacgt cgaaataaaa aataaacaaa aagagtaaga
     4501 tgaacctctc tatagagtgc tcgtcttgct ctttttttgc gaaaaacaaa taatttttga
     4561 atacagcatc cctcgcttta taataaacga tagaataaag ggagtgaatc aatgatgagt
     4621 caaacaaaag tcgaacgtta tttagaagaa caaaagatca gctatcaacc acttgatttt
     4681 agtcatgtgg tagaagaatc tttcacagaa gaactacaaa aaagagggat tgatccagct
     4741 ttgatatgta aaactctcgt agtgaaagga aacaaaactg gggtgatcat cgcattgatt
     4801 cctttgaacg atcacttaga ttacaaaaaa acgagacaag tgacgaaaga tcgtaaagtt
     4861 ggttttcctg gaatggattt tgtattaatg catacaggat atccccacgg agcaaacacc
     4921 cctattggaa taaaactcgc acatcctgat tattgttttt tagcagacag ttctttaaaa
     4981 gaaaaagaag aagtaattgt gtcaagcggt gaaatcggga gaagccttcg gataaaagta
     5041 gccgatttag aaaagatcgt ccatcctatt tacgctgatt taattagtta agggacgaga
     5101 agagaagcta agcagagcag agacctcaaa cttgagatta tatctgcttg gtacttggtg
     5161 catcctgtat tatctgttac aattgctttg tacatactgt actatcaatc caatgaaaat
     5221 atatttagca gctatatttt ttcattgatg tgcaagtgat gaaatcgttt ttaataaaaa
     5281 agatacagag aaaacgcgag gtgtaaaata tgaaaattac gtatcatgga cattcctgtt
     5341 taacaattga aatggaaaat ggccaacgcg tgatgattga cccgtttatt acaggaaatc
     5401 cattgacaga cttaactgca gaagaagtaa agacagattg gttgctgatt actcatggtc
     5461 atagtgacca cataggagat atgattccta ttgcaaaaga aaatcaagcc acaattatca
     5521 gtattgttga gattgctgat tatgcgaaca gcagaggagt agattcattc ggtatgaata
     5581 tcggtggaaa acatgcgttt ccattcggcg tggtaaaatt cgtacatgca cagcatagtt
     5641 caagctatga agtagacggt atcgttcagt atatgggcga gcctagcgga attattattc
     5701 aagcagaagg aaaaacgatt tatcatgcag gggatactgc ttatttcagt gatctaggat
     5761 tgcttgcaga agaattcgat attgatgtcg ctttcttgcc gattggcgat aactatacga
     5821 tggggccaga agatgcagca aaggctgcgc aaacaattcg tgccaagaaa gtagttccaa
     5881 tccattacaa tactttccct gtcattgaac aagatcccga atcttttgta gagttactgg
     5941 aagatagtga aggaaaaata atgagcgtct cggaaacgct ggaagtgtag gcagaaaatg
     6001 gcgacaaaac atgatttgat tttagaatat attgaaagtt tacctgttgg caatcgaatt
     6061 tctgtaagaa gcatcgcaaa agaattgaat gtcagtgaag gaacagcgta ccgtgcaatc
     6121 aaagatgcag aaaatgtcgg actagtttca acaattcaac gagtagggac catccgtatc
     6181 gaacgcaaat taaaaaaaca tattgaacgt ttgacatttg gcgaggtcgt caggatcatt
     6241 gaaggggacg ttttaggcgg ggcggcagga ttagacaagg tcttgaataa attcgtcatc
     6301 ggcgcgatga ctaaacatgc catggaacgc tatattacgc caggatcact aatgatcgtc
     6361 ggcaaccggg cggaagtcca aaaattagca ttggaagatg gcgccgcagt attgatcaca
     6421 ggaggattca atacgactgc tgagattgca aagctagctg atgatcttga aatgccgatc
     6481 ttacgcacct cttatgatac atttacagtc gcaaccatga tcaaccgagc gttaagcgac
     6541 caattgatca aaaaagatat catgcttgtc agtgatatct atatgccagc tgaaaagact
     6601 cattatttgc agcagacaga tacgatttct gattatcagc gtttgtcaga agaaacgcag
     6661 cattcgcgtt ttccagtcgt caaccgccat catcgtttga tggggattgt tacggcaaaa
     6721 gatgttttag ggaaatcgcc aaaccagatc atcgatcggg tgatgacgaa agaaccaatc
     6781 agtgtaaaga aaacaatgag tattgcctct gttagccatc aaatgatctg ggatgggcta
     6841 gaagtcatgc cggtcgtttc cgatgacttg acattggaag gcttgattac ccggcaagat
     6901 gtaatgaaag cgatgcagct tgtccagcga caaccgcaga ttgcagatac gatttcggat
     6961 cagatatctg gaaatatcca tacgattgat actgaccgtg aaggaaatcg tttggaaaac
     7021 cctaagttca aattcgtagt agcaccacaa atggtcaatg gcgttggaac catttctttt
     7081 ggcgtactta gcgaaatcat ttctgatgtc gcacaaaaaa caatggtgat gaaccaaaaa
     7141 cgaaatatct tgattgaaca agtgaaccta cattatttgc ggctgatcca attggaaagc
     7201 gaattagaca ttcgtccacg tgtattggaa atcggcagac gttccgctaa actggatatc
     7261 gaagtgttta ttgaaaacac catcgtttcc aaagcaatcg ttgtttgtca agtaatggaa
     7321 cgttcttaga aagcaggagt gaaatgacag tacagcaaga aatagtagat acaataaaaa
     7381 aatacgataa aatcattatc cacaggcatt tgcgccctga tcctgatgca ttaggatcac
     7441 aagtaggatt agccgaacta ttacgggaaa gtttcccaga aaaagaaata cgtcaagtag
     7501 gtgaaacgat tgaagggctt cgctttttag cagaaatgca agaagttgaa gatgtcttct
     7561 accaaggagc gctagtcatc gtgactgata cagctaattc acccagaatc agtgatcaaa
     7621 gatatcagtt ggcagaaaaa ctgatcaaaa tcgaccatca tcccaatgat gacccttatg
     7681 gagatcttgt gtgggtaaat acaaaagcaa gtagctgtag tgagatgatt gcagaatttg
     7741 ctttgatgtt tcctgaatct ttaaagatga acgttaaggc tgcacggtta ctttatgcag
     7801 gaatcgtcgg agatacaggg cgctttttgt atccctccac gacgtcccgt acattagaga
     7861 ttgcttcgat attgagaaat tatccatttg atgcaagtgc tttgaacaga gagattgaac
     7921 aaatgccgat gaaagtcgct aaactttccg gttatcttta tcaaaatatc caggtggatg
     7981 aaaatggtgc aggaagaata atccttccgc aagaaattct tgataaatat ggaatcgatg
     8041 attcagagac agcttcgatc gtttcacttc ctggagtgat tgacgaagtg ctggcttggg
     8101 ggatttttgt tcagcagcct tcagggtatt accgcgtacg tttacgttct aaggggccga
     8161 tcatcaatga attggctaaa cgccatcatg gaggaggcca tccattagca agtggtgcga
     8221 atgcaaaaga tttagcagaa gtagaggaaa tctataaaga gatccaagat atatgccaat
     8281 cgtataagag cagaaaaaca gaatgatact tcgttcgaaa acgtgccttt tgtagtaaaa
     8341 atcacaaatg tatcttttca agatcttctt tttccgttac aatagtgctc actaaagatt
     8401 ttaaaggaga aagaagatga cattaccaaa ttgcccagaa tgtggctcag agtacacata
     8461 tgaagatcga ggcttgttga tttgcccaga atgcgggaac gaatggaaca aagaagaaca
     8521 agaaactgtc atagaagacg gattagtggt attagatgca aatggaaacc gtctgttaga
     8581 gggcgatagc gtgacagtga ttaaagactt gaaagtaaaa ggtgcgtcag gtgccattaa
     8641 acaaggaaca aaagtcaaga acatccgttt agtagaaggc gatcataata tcgattgcaa
     8701 ggtagaagga tttggaccga tgaaactgaa atcggaattt gtgaaaaaga attaaagaat
     8761 attcaaggtc ttttcacatt cttttcctac tactaatgat aaaataaagt aaacgaaaag
     8821 gagtgaaacg aaaatgtatg gttatggaat gtatggtttt ttagatccga catttctttt
     8881 ggtcattctt ggattagtta tttccggaat tgcgtcagcg tacgtcaata gtactttccg
     8941 aaaatacgat caagtgagaa gcaaaaacaa tgtgactggt acacaagcag cacaatatat
     9001 attacaaagt caacagataa ataatgtcgg tgttcaacaa attgccggag atctcacaga
     9061 taactacaat tcaggtaata agatgttgag tttgtctcaa gcaactgcac agtctacttc
     9121 agttgctgcg ataggtgtag cagctcatga atgcggacat gcggtacaag atgcttcggg
     9181 ttatgtccca ttgaagattc gtgcagcact tgttccagca gctaactttg gttcgatgat
     9241 ttcgttcccc ttgattatgg taggtgtatt gcttagctgg aactctacgt taatcaatat
     9301 cgggattttc gctttttctt tagcattact tttccaattg gtgacattac cagtcgaatt
     9361 taacgcgtct cggcgagcaa tccaaatttt gagtgaaggc ggactactga cggaagaaga
     9421 agtaccgatg gcaagacacg tattatttgc agcagcactg acttatgttg cagcagcgct
     9481 ttcgacattc ctgcagctgt tgcgcttgat cttattgttc ggcggcaacc gaagagatta
     9541 aaataaagaa gttgtgaaat cagcgctttg atctgagcta ataccgagag ttagctgatt
     9601 gaataccatt tatttggaat
//
LOCUS       DACEMO010000103         9427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEMO010000103 DACEMO010000000
VERSION     DACEMO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.106"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(259..1023)
                     /locus_tag="IW728_001817"
                     /pseudo
     CDS             complement(259..1023)
                     /locus_tag="IW728_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
     gene            complement(1134..2003)
                     /gene="mdcB"
                     /locus_tag="IW728_001818"
     CDS             complement(1134..2003)
                     /gene="mdcB"
                     /locus_tag="IW728_001818"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ1025173.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(2028..3104)
                     /locus_tag="IW728_001819"
     CDS             complement(2028..3104)
                     /locus_tag="IW728_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ1025174.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(3128..3721)
                     /locus_tag="IW728_001820"
     CDS             complement(3128..3721)
                     /locus_tag="IW728_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ1025175.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(3725..5401)
                     /gene="mdcD"
                     /locus_tag="IW728_001821"
     CDS             complement(3725..5401)
                     /gene="mdcD"
                     /locus_tag="IW728_001821"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ1025176.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVAIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(5391..5690)
                     /locus_tag="IW728_001822"
     CDS             complement(5391..5690)
                     /locus_tag="IW728_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ1025177.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(5792..7438)
                     /gene="mdcA"
                     /locus_tag="IW728_001823"
     CDS             complement(5792..7438)
                     /gene="mdcA"
                     /locus_tag="IW728_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ1025178.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(7445..8356)
                     /locus_tag="IW728_001824"
     CDS             complement(7445..8356)
                     /locus_tag="IW728_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1025179.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            8476..9333
                     /locus_tag="IW728_001825"
     CDS             8476..9333
                     /locus_tag="IW728_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1025180.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
BASE COUNT         2651 a         2049 c         1684 g         3043 t
ORIGIN      
        1 ctaaactaaa atagtgcttc ctatttttcg tttggttgtt cttattatcg cagatgcaag
       61 cttttaaaca ccatttagtg gagaggttgt gacagagatg ttcagccttg agcgataaga
      121 agcgattttg aaaaatcgct tcccatattt tacaaaatcg tgccttattg ccgataggct
      181 atctctggca caccgtttat tcagaaaaag gagctgcgac aaggcttttg tcacaactcc
      241 gttcataaat atttcctgtc aatccagcat aatcggctga ataaaatcgg cttgccatct
      301 tttttctttc tcagaaaaat caaaatacca tatctgagaa aaatactgtc gttgtgtctt
      361 acttccttag atcaatcgcc catcttttcg atcgactgaa taatcacagc atctgaaatc
      421 aattcggatg gcaaaactat tttcgtggat ctgtcgataa tttcctactt cctgaatagt
      481 tattttttta gtatgatttc ttacaccgtc tgctttgttt ttttgcagaa ctttctgatg
      541 ttcggcaaac agcttgtctg tataacagct tctggctttt tcaggagttt cttcttccca
      601 agcggattgg atcttcatga atatcttttg gatttccaga aactgtgctt tcttttcttt
      661 agccgttcct gacatgcggg acaatatctc cgctttcagc cttttctttt gtcttttatt
      721 cttgacgata gaaaaagcag aaacccctcc tactgacaaa acagctaatg caagaaatcc
      781 cgtttgctca taactagaag gacctgtacc tggaccataa tacccataag aatctctgcc
      841 gtaagaacga ctgccaaaat aactgccccc atatgaacga ccaccaaatg aacctctgcc
      901 cgaacttcga ccgctagatg tacctccacc atgaccaccc gcgacagcga atccttgggt
      961 cggcagagca aacaaacata ggcccactaa caaaatgatt gctaaataat tgattttttt
     1021 cattgtcttc ctcgttatct tttattacaa aaaactattt ttatgatagc cttgctaaaa
     1081 ggaagagtca ataaccagat gcataaactg tttttttagc aattttgtct tatttagtca
     1141 ttgagtaaca tcggacaaat gtatgtgtcg gcagtccata attcctctat cttcagcatg
     1201 aacaagcttg cagacaagag atctgcgctt cctccaggag agatttggtt ttcctgacaa
     1261 aagtgcaaca gtgctgtaaa tggacgattt ggtaaagcac tttgactagc agcatgagca
     1321 agtttttgca tttccactaa cgttttctga tcactgcgat gcaatacgca tgtgtcatct
     1381 agcgaactca tcaaaactaa caacatatgc atttgtttct gtacatatga atcattatct
     1441 agctccttag ctgcttgaat tgcaagcctg atatgcggat accctaatat cgcttcttca
     1501 tatgcaccat ttacgccata tttttgcttc accctatgac catgagtggt ttttttatga
     1561 cgatattttg tatcaggaaa agcagcaagc ttgctgacat catcaagtat tttcgataaa
     1621 gaaaaaggct gttccatact gattttttgt gcaatgacac tgatcaatag ccccattgcc
     1681 caaatcgctc ctttatgggt attgatgcca tttgtagcaa tcagcattgc cttttccgct
     1741 tttcgcccaa tctcagcaat tctttctctt aactcctgat caatagcatg atgccatgct
     1801 atttgagcca tttcttcaaa aaaaggagtt agcgtcttag cagacttaag aaataaagca
     1861 tagtccatat cggaatgtgc acccgcatcc agtgcatcca ctaatccagg ttttggactt
     1921 aatgttactt ctgcgatcaa tgcttcctct accaacgcag cgagttgctg agtcactgct
     1981 ttttttcgtt cgtcatttat catgacactc actccttctt cctactatta cttgctgaaa
     2041 ccttttttga tagctaccca aatgatatga agtaaccagc ttaaagcaaa tgcacaactt
     2101 gccgtcatta gataagctac aagaatcatg actcctgaag gtaactgcag ccaactatct
     2161 ttaaacaata ataacagtac ttggaaaaca aatacattgg ctaaatacgt acgataagca
     2221 taaacagaca accatttgaa caatggcaat tttggcgaat cgtttacaat cattttggaa
     2281 gcgcctatga agacagcaaa gatgatgact aaactgtata aactttggat cgtattcaag
     2341 taaggtgcat tcgcaaagct caagtctcct ggatgtgcga gcaatgaacg gacagaaaag
     2401 aacgcgataa ctaaggcaac aggtaagaaa agataacgta ctctgtccaa atattgatag
     2461 actttttcgt gataaacaaa tgcggctacc ccataaatca tgaaactagc aacaaaccga
     2521 tctaataagt accagctgtc atgataaggc ccagtcaata cttgtgtttg atagaacaga
     2581 taccatgcga catataaaat cgttgaacca atcaatactg gccatacata ttttttatta
     2641 gcaaatactt tgtaaccgag ccaaacaaaa aacggcatga acaattggat ctgcagcatc
     2701 ataacggtat accataaatg aggcgctcca tcacctgtga catatttcag taaaaagctt
     2761 gcagtatccg tatacggagt ttgttgttgg actgctggaa atagccaaag ataagccgtc
     2821 gtccatagaa gatacggaac gactagttca aaaaactttt ctttcaaaaa ttcccaataa
     2881 gacaaatgct gacttgtttt taccatatta taaacaattg caaaaataaa cattggtgct
     2941 gtgtatttaa cgatgacgta aaaggtacca agaaaagctg tatttgttgc gctgtggctg
     3001 tttgccaaag caaagctcaa tatcgtttgc atcataacag ccgttacagc tgatactttc
     3061 tggaagtcac ttacgttgat tttgattttt ttgttttcaa acatctcttt ctctctcttt
     3121 ttctttatta ccaaagttct ttggttaatt taggtccttc gactgttttc aatagaattt
     3181 ttttctctgg ctctctcaaa tactctttta gtgaaaaacc gccttgccga gtcactactt
     3241 gggtatcgaa ttgctcaaaa aaagcaggat gcgattgaat tacctgaatt ggcaattcaa
     3301 tcggtgaatt tgcgtacagc agcaaatcaa aatcactggt ttcttttact gccggaatcc
     3361 cagtcgataa ttcaaatcct aagctgccgc cgattcccca tttacatcct gataatatgt
     3421 ttctagcagc atgaaattgc tgataaacag gataatccat gattttctct tgtctatacg
     3481 attcttgttc gatgatgtcc cacggatgaa tgacttgttt gacggcatgt tttgagattt
     3541 ctgttgacca tctctgattg cgggcaggtc cacgtaaacc aatcggtagt atttctgcgt
     3601 gatccgtcga cgcacgacga actgttccga acatcttttc agttagaacg acccattcag
     3661 gcaaggtgaa cccttctatt tcatgtttat caaaaaggac aatatcatgt gcttgaatca
     3721 ttttttatgc atcccattct tcgtccattt tggcacggac tttatttgtt tcgacacgtc
     3781 cgaatgtttt ggctgtttcg tttgtgtatc gatttgtcag catcgtgtca tttgtattgc
     3841 ggacatcttg aatggcttca ttgattgctt cgatgacttt gctgacattt tgttctgaag
     3901 cagcttggtc ggtgatatct tctaggaaat gatagatcgc tcctagtttt gtataattat
     3961 tgatatcgta agcgatagct ggaacttttt cagcagctgc ttcgatttca gcaaccgaac
     4021 gttttgtcac acgtgcagca ctttctttag acatcgcttg gacggtaatt tctttattgt
     4081 ttaaagcaat caagcggctt gattgtaatc catgtgctaa aaatgcacct gaaatagcat
     4141 ttcctgcaat caatccaata actggatgtc cagcttgacg gagttttgca taagcagcag
     4201 cactattagc caaagccaca tggataccga tcaattcttc gttatagccg tatgcttggc
     4261 ttggtacatc gatcacaacg atgattggcc gtttcacttt tttgtcttta tcttcttcgt
     4321 aaacgtgatc caaaatttgt cccatgcgga aaccttcaac taaaccaact tcaccgtttc
     4381 gtacacggta catcgggttt ttaggatcag gtacgattga tgtcacgatg gcttcttcta
     4441 tgaatttact tgatttacca taatacacag tactaataga agaagttgga tttttcatgc
     4501 cagttagtgc cgcaaaccaa tcgtaaccaa cacttgctgc agctggttca tttccttctg
     4561 ctacttctgg taagtcaatt gattttgctt gatggttagc aattgcttca ttgtaatcgt
     4621 caatggccat tggttttgaa agatctaaag aatcaagtaa tgataaataa aatgctgagc
     4681 tttcactgcg atgcgtgtct tttcgttcgt ccattgctgc gatgacagca gctttgattg
     4741 cttctacact atcagtcact aattcatcaa tgatttttga ttctacccgt tgacgagatc
     4801 ctaatgtatt ccaaatcaag tctttgtcac tagaatcaaa ttctcgaacg cctgcttctt
     4861 gttcaataac ttctggtcca ttcaaaccga ctctcgcttt ttttgttgcg attaaataac
     4921 tcattaatgc tgaagtaatc gacataccgc caaatgatcc tacacgtcct ggaatcaaac
     4981 cgatgactgg tacatattgt tttagagcaa tgattacatt tccgatttct gaaatagata
     5041 acagtccgta gtttgcttct tgcaaacgaa caccacctgt atctagtaca atgactggaa
     5101 agatctcatt tcctttttta ttttcttcta acacatgttc aagtgcagca acgatttttg
     5161 caccggaaac ttcaccgata ccgccacctt ggaattttcc ttccatagcg attgctactg
     5221 cttgtttgcc attgatcgtg ccacggctga caacgatccc gtcatcactt tcagggacga
     5281 ttccttgtgc tggtaaactt ggcgcgatga tttgatcgaa tggatcaatc aattcacgtc
     5341 cattgtcaag tagagcgttc actcgttctc tggcgtttaa ttctacaaaa ctatttttca
     5401 tctgaaagtt cctccattgc ttgcgtcaat cgtaggtaca caactcctgg tgtagcaccg
     5461 aagtcattga tcacgatatc tgctgaaatt ggataacgat caaaaaatct cgttaataca
     5521 ttgttccata cttcgccgaa gccatcgctt cctgtacaaa tttttacagt agattctgtt
     5581 ttttctgttg gaaacatcaa gatttccaaa tctcctgaac cgaccactcc tacatggatt
     5641 ttcttattga tcggctgttt cgttggatat ttataagcta attgttccat ggatctctcc
     5701 cctttttgta aaacagagca tacacgttct gcttattcgt ctttcttctt ccatctgggt
     5761 aaatggcgac aaaaggtttt tctcgccaat tttatgacca agttctgaat ttcattggtg
     5821 gttcatacag gccatctgac cattcgatca aatcatcgat ggtttgtgca gctaataatg
     5881 aacgtttcgc ttcactacgt ttgatgccta gatcttctgg taatttcacg atgccggctg
     5941 cacgcaagct atctagtgat tctttcgtgc tagtcattcc aagaggtgta acaccgccaa
     6001 ttgctgcaat tgctgcttgt cgttcttctt ttgtttttgc tttgtacaaa taagcaattc
     6061 cttcttctgt cacgatatgt gtagtatctt cgctataaat catcactggt gcattggcta
     6121 agcctgcttg tttttgaaca gctagtgcat ctaattcttc gacaaagact ggttttttat
     6181 ttgaaccata tgtttcaacc atttgaacaa ctaatttttg gcctttccct aatggatcat
     6241 cattgtcgcg aagtgattgc catgctggtg tagaatgacg acgaccgcca ggattatgtc
     6301 ccatgttagg cgcaccgcca aaaccagata aacgtccttt tgttacggta gaagagtttc
     6361 cttcgtaatc taattgcaag gttgaaccga taaacatatc gatggcatat tgtcctgcag
     6421 cttgtgataa tgttcggtta ctgcgcatcg tgccgtcttt accgacaaaa aagacatctg
     6481 ggcgagcagc aatatatttt tccataccaa cttcgccacc aaaactatgg atgctttcaa
     6541 cccaacctga ttcgatggct ggaatcatcg ttggatgtgg gttcaatacc cagttcttcg
     6601 cgattttccc ttttaaccct agtgattcac cataagttgg cagtaacaat tcgatagctg
     6661 ctgtattgaa tccaataccg tgattcaaag attgtacgcc gtgttctgcg tagattcctt
     6721 tgattgccat cattgccatt agaatttgca attctgtgat gttttgagga tctcgtgtga
     6781 ataaagcttc taattgataa ggtttatctg ctttcacgac aacgtctacc cagtcaccag
     6841 ggatatctac acgtggtaaa gaatcgacaa cttcgttaac ttgaacgatc acgataccat
     6901 ctttgaaagc tgcagcttcg atgattgttg gtgtttcttc cgtattgaaa cctgtgtaca
     6961 agttgccgtc tttatctgct ttatctgcag ctactaaaac aacatttgga atcaaatcga
     7021 tgaataagcg tccataaagt tccaaataag tatggatatc acctaatttc attgtgccgt
     7081 cttcaatcat ttgggcgaca cgcaagcttt gtgcaccagc ataagagaaa tcgatcttgc
     7141 ttgcgattcc tttttcaaaa atattcaaat gttctggtcg tgagatacta gacatgatca
     7201 aatgcagatc attcactttt tctgcatcta ctttttcaag actttctgaa agaaaacttg
     7261 cttgtttttg attgtcgcct tctaatacaa ctttgtcgcc gggagcaatc agtttttcca
     7321 ttacttctac gattttttca gtttctacat acttgccatt gagcaagttg ctgatttttt
     7381 ccaatcgctg cgcttttttt tcgcgtttag aagcccagtt gcggtttggt ttttccataa
     7441 ttagtcatct ccatttttga tatagaaacg tggcgtcatc aataccatgt tggtctagat
     7501 tgatcgtacg gatcccttta tctacaccaa attttgtatg aatcagcttt gtcaaagtat
     7561 tgccgggggg aaattcaatc gtcagatcca ttcctaattc ttttgccaag tccatcattt
     7621 ggttccactg cacagggaaa gctaggtttt ccaacaaatc tttacgaatc tcttctgcgt
     7681 cctgagtggc tcttccatcc gtatttttta tatacaaaca tctagcatca gatagatgga
     7741 cagactgcat atacggcttg aatttcttga ccgtatcatt catcaagaga cagtgagatg
     7801 gcacagggac tttcagcagc ttcgctgttt gcgcttggtg ccgtttggct tctgccaacg
     7861 taacggataa accttcgata ctcccagata acgtatgttg catcggacaa ttttcatttg
     7921 acagataaac tggatggtca ggatcaaatg tttttgctac aatttcctca gcttcgcttc
     7981 tcgtcacacc tactactacg cccattgcat aaccgcttgg ataagcttct ttcattaaga
     8041 ctgcgcggtg atagactagt tccgctgcat tctcaaatct caaagatcga caagcaacag
     8101 ctgcacaaaa agcaccaatc gaatgtcctg ctgcaatatc aggctgaata ccgttttttt
     8161 ccatttcatc taaataaaat actgcttttg tcagtaatgc tagctggata aaaaccgtat
     8221 cttgatagtt ttcttcttta tcaactaacc gataccccgt cacattttct aaacgttcca
     8281 tgtacatttc agggatatcc gctaacatcc ctaacttctg actgccttgt ccaggaaata
     8341 agaaacctgt actcatgagc ctcgcctcct cagcacttac tttttccact gctatcttag
     8401 tcaatttctg ctataataaa aaatgccaaa aaaagaaaaa accataacaa aaaggtatgg
     8461 atgggatgga taattatgaa attgcttcat ctgcagtatt ttaaaaaagt attcgaaaca
     8521 aaaaatgtta cccaagcagc caaagaatta tttatttctc aacctgcctt gagccgtgcg
     8581 atcaaacatt tggaaaacga attaggtgtc cctttgtttt accatagcgg cagaaacatt
     8641 gagacgaccg tttatgcaga agaattttat ccatatgcag tcaagacatt agacacatta
     8701 gaagaaggaa tcaaattatt gtcttcggtc aatgagaaga tcgttacttc cgtcatattg
     8761 tatttagagg tagcctctgt ttctattccg aacttggtcc gaatttttat ggaaaagcat
     8821 cctgatatcc aattaacgat catgcaacat catttaccag atgatattac cgatcctgta
     8881 ctgtacatca cttcggagat gaagcctggc atgaccaata ttcctatcat gaaagaacct
     8941 gtttatgtcg ccattcctaa gaaacatcct cttgctaaaa aagaacatct gcaattagaa
     9001 gatatccaac gcacccccat cttgatgctg tcaaagagca acgccttccg tcatacactg
     9061 gatgcagcac ttgaagcgaa aagtatcgaa ttgacgatca gttcgacgac agatgatcct
     9121 gcgacattac gttctattgt caagcaagga ttagctgtta gctttttccc aaaagtttct
     9181 tggtcttacg ataaaaatga tcctttcgtc ttgcgggaaa tcaaagattt gcccttgaca
     9241 cgaacaattt atttatcttc tagcttcaca gaagatcatc ctttgatcaa aatgattgca
     9301 aaaacgatgc gggaatttta tttaggaaaa taaaaaaaaa agagctggga caaaattctt
     9361 gtcgcagctc ttttttcgaa caaacggtgt tccagaagta atttcttaga aaataagccg
     9421 aaatttc
//
LOCUS       DACEMO010000104         9379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEMO010000104 DACEMO010000000
VERSION     DACEMO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.107"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..750
                     /locus_tag="IW728_001826"
     CDS             <1..750
                     /locus_tag="IW728_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1025181.1"
                     /translation="LNKRADTNALKKKIGIFSENSVMGFIIGLGLGFAAAYGVSGSLN
                     LAIQTATALSLFPMISKMFMQSLSPLADAMSEFMKKRFKDREVYIGLDWPILAGRSEI
                     WVTAILLVPVFIGYAIILPGNQVLPLAGIINYSIAVGGLLLTGGNLARMLILGIITMP
                     IYLYGATYLAPILSDLAEKSGAVEGIKKGQLITWSSIEGPEFRVLFAEAFNRNILAIV
                     GCVIFLGLFVLLYKYMIKAKVPSERYAELQK"
     gene            772..1032
                     /locus_tag="IW728_001827"
     CDS             772..1032
                     /locus_tag="IW728_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025182.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            1046..1798
                     /locus_tag="IW728_001828"
     CDS             1046..1798
                     /locus_tag="IW728_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1025183.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(1892..2497)
                     /locus_tag="IW728_001829"
     CDS             complement(1892..2497)
                     /locus_tag="IW728_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1025184.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(2550..3524)
                     /locus_tag="IW728_001830"
     CDS             complement(2550..3524)
                     /locus_tag="IW728_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1025185.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(3902..4681)
                     /locus_tag="IW728_001831"
     CDS             complement(3902..4681)
                     /locus_tag="IW728_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1025186.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(4748..6019)
                     /gene="celB"
                     /locus_tag="IW728_001832"
     CDS             complement(4748..6019)
                     /gene="celB"
                     /locus_tag="IW728_001832"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1025187.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            6172..7569
                     /locus_tag="IW728_001833"
     CDS             6172..7569
                     /locus_tag="IW728_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1025188.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            7650..8090
                     /locus_tag="IW728_001834"
     CDS             7650..8090
                     /locus_tag="IW728_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1025189.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            8238..8564
                     /locus_tag="IW728_001835"
     CDS             8238..8564
                     /locus_tag="IW728_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1025190.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            8565..9314
                     /locus_tag="IW728_001836"
     CDS             8565..9314
                     /locus_tag="IW728_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025191.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         2995 a         1688 c         1928 g         2768 t
ORIGIN      
        1 ttaaataaac gagcggatac gaatgcattg aagaagaaaa ttgggatctt ctctgaaaac
       61 tctgtaatgg gattcattat tggattgggg cttggctttg ctgctgcgta tggcgtttca
      121 ggttctctta atctagcgat ccaaacagct acagcattaa gtttgtttcc tatgatctct
      181 aaaatgttca tgcaatcttt gtctcctcta gcagacgcta tgtcagaatt tatgaaaaag
      241 cggtttaagg atcgtgaagt atatatcgga ttggactggc cgatattagc tggtcgttca
      301 gaaatttggg ttactgctat tttacttgtt cctgtgttta ttggttatgc cattattttg
      361 ccaggaaacc aagtattgcc attagcaggg attatcaact attcgattgc ggtcggcgga
      421 ttacttttaa caggaggaaa cttggctcgt atgctgatct tgggaatcat cactatgcct
      481 atttatttgt acggggccac ttaccttgcg ccaatactca gtgatttagc agaaaaaagc
      541 ggagcagtcg agggaatcaa gaaaggacag ttgatcacat ggtcatccat agaagggccg
      601 gagttcaggg ttttatttgc ggaggcattt aataggaata ttctcgctat tgtaggatgc
      661 gtgatttttc taggactgtt tgttctactt tataaatata tgataaaagc gaaagtaccg
      721 agtgaacgtt atgctgaact acaaaaatag cattgaaatg gtgggaaagt aatgaaaaga
      781 gaagagacag ataaaataaa atggacagtc gctctttgtg gtacacttct gctatttcta
      841 tatggactct ttactcagaa tatcatcatc aatctattgg tcattttttt cgcactagtg
      901 atctataaat atggaaatca cgttttattc agagagtacg atgaaaaaag aaaacggaaa
      961 atagaagaat caatgaaaat aaaagaagct accaaagaaa tattgagaga aaaaagtttt
     1021 ataaaaagat aatggagggg tcattatgtc agatggacgt atgttatttg agagagaacg
     1081 acaggacatg gcacgtatcg taagagtgat tttcgaaagg aaaaacacaa atgtcgcagg
     1141 agggaatttt tcctttaaaa ccactgatga tcaagggaaa gactacatca tcatgacgcc
     1201 gaccatgatg tcagaagcct atctaggaga gatttctcct gcacaggttc tagtagtaga
     1261 accaagaacg cgtagaatcg ttgacggagt cggtcattta actcgtgaaa tcaatatgca
     1321 tgaagcgatg tatgagacta atccagctat caaagctgtc ctacatgctc atgcgcctaa
     1381 tgcaatgttc tgggcgacta ctgggttgcc tatgcctaat ttgacagaag ccacagctaa
     1441 agtaaaagag attcctgtca tggactttga gcctaattgc tctgaagaac ttgctgaatt
     1501 gacaagcgac tatgtaaaaa aacagcatct cgaattaccg catatgttgc tgctcaactc
     1561 tcatggcgta ttgatcaaca caggtggaga atcaggaatc gaagctatcc ataaagcact
     1621 gcaaatcctt gatacagttg aatggaatgc agaaattgcc tacaagcaga cgatttttca
     1681 agcattaggg attactgacg gtttttattc aaaaggtgag aaaatcgggg gattggaaga
     1741 gttgaaacat aaaacggcta tttataataa atctgttatt gcagctggtg gagactaacc
     1801 tatctgacac aaaaaactac gactttcaca aaatctgatc cagatcgata tctgcttcag
     1861 gttgtattat gtaagtcgta gttcttttct tttatttagt taagaggtgg tctgtccatt
     1921 ccctcgcttg cttgatttcg tccatcgtca actgatgacc tgttacagtt agctgaatag
     1981 tcacatttgc ttggcgtgtt tcgaattgat cggccaattc tttaaaggaa gcaggggaaa
     2041 cgattggatc gttatcacca taagaaatcc agacggtttt atcctctaat gtggaacggc
     2101 tttgatgaac acctagagac atcggatgga aaaacaaccc tttatttagc tcggtctcac
     2161 gctccaataa taaatgagca gcgatgtttg cgccatttga atagccgaca agcagccagt
     2221 cacttaacgg tatcagttct ttttcgctga tttctttgat tttttcatgg agtttatctg
     2281 tttctttttc caaactgtcc aggtcgaatt gattcaatcc attacgcttg aaaaaacgat
     2341 tcatcccatc ttcttggacc gtgccacgaa aagaaagcaa tgtactgttt ggtgcaagaa
     2401 agtgagcgat atccagtaaa gaatgttcat cgccacctgt gccatgaagt aagatcaaat
     2461 gcttgccact tgttcctttt tcaaaaatgt aggtcatttt gttccctctt ttctgtttct
     2521 atagatgcaa atgaagtagc atgaagagat tattcacgtt ttttatttgc atcacttgta
     2581 tcgaaaggac ggacataagc ttcaatggct tcacgacgtt cttctaagaa tggaggaaga
     2641 gatagttttt ctcctgctga ttctgctggt tcatcttcaa ggaatccagg accatcagtt
     2701 gctaattcaa acagtacgtg atgagcggct aggaagtact cagattggaa atagaaacga
     2761 tcgacgaaac cagaatttgg caattcaatc ttattgatta cttgaatcca gtattccaat
     2821 gcttctttgt ctgctacacg caatgctaag tgatggatat ttccaaatcc ttcgattgct
     2881 tttggcaagt ctgtccgatg ttcaataatg atgcttgcac catttccccc ttcaccgatt
     2941 tcaaataagt agaaatctcc tgaatggtct gtttctttga atcctaagat tttttcaaga
     3001 atcaagcgga tatgctctaa ttgactgaca cggacaaaga caggtccaag tcctgtgatc
     3061 gcaaactctg tcggtacatt gctgtttttc caaggaatgc cagctgctac accttgattg
     3121 ttttcgtcag agatcaattg ataatactga ttatcgaaat cttcaaattc gatatatttt
     3181 ttgccgaaac gttcattgat tgctgaatga gtaacgtcat atttttcaaa acgttccaac
     3241 caaaagtcta atgctgcgtc acttggcaca cggaaagaag cacgagagat cgtatctgtt
     3301 cctttttttc cttttggctg atttggaaaa tcaaagaaag tcatatctgt tcctggcgca
     3361 ccagtatcgt ctgtaaagta aagatggtaa gtttcgatgt catcttggtt aactgttttt
     3421 ttgattaagc gcattcctaa aatatctgta aaaaagcggt agatttttgt agcgctagat
     3481 gtcattgctg tgacgtggtg aattcctctg atttgtgtat ccatgataaa tttcctcctg
     3541 ttaacttaca aaaagtaagt agttgattta gacatagtat agagggtgca tctctatttt
     3601 gcaattaatt tgtcttctaa agtatcagga aggaatcaag aatggaaaag aggagggacg
     3661 gatcaataaa ataaaaagac ttacgactag ctgtatcgat ttcaaaaaaa ctagctttaa
     3721 aaagctaact ttagaatcat tacgggtggt cataagtctt tttatgctga gttatttttt
     3781 catttttgag ctaatgctta ggatagaata gaatgttgat ttcacgatct cttcttgtta
     3841 cttgaaactg cttaccactg tcacgatctc ttggtaaacg gctttttcac aacctctttg
     3901 gttaagttga acgcacatcc agcacagaat agttccgaac atcaaatcgg tttcggctgg
     3961 aagaatattc aatcggctgt ccatctttga gatagacgac ttgctgaact tccagtacag
     4021 gatcgttttc tgcacagttc aaatattgct gatcatattc atcactttta tctgcagaaa
     4081 tagcacgata agcccctgca aaatggacat tcaattcttg atgaagatga tcatagatag
     4141 aggctaacaa atgttctttt tttaaacccg gcacaagatg gactggcata aaggtatgct
     4201 ctaaaacata tggctcgcca tctaagatcc ttaaccggtg aatattgtag acaggttgct
     4261 cggcatcgat catcaatcgt tcttggatat attgattagg gaattcaaca tcaaaatgaa
     4321 tgatttgact cttcaaagaa cgatgtctgc gtttcatttg gagactcaaa ccttcatact
     4381 cgttagcggg cgatgtgtct ttttgcataa atggatgatt caagattttc gttcctgatc
     4441 ctcgctgcga atagacaagt ccttccattg ctaagatatt gatggctttt ttgattgtca
     4501 tccggctcgc accaaattct tttaccaagt cgacttgatt aggcaaaaaa ctatcgagag
     4561 gatactcttt tgaacggata cgttctctta aaatatttgc gatttcttcg tattttggca
     4621 cgttattcac ctcttctaga gtataacgaa agattatgct tccgacaact cgaagtagca
     4681 tctggaacat tgtttattcg aaaaaagagg tcatgacaag tttttgtcac aaccccttaa
     4741 tgatctatta agcaatatct aaatctggtt gtttatctag tactttcagg aatgggtacc
     4801 agataaaggc aacgatgaca aagtcgatga cttgcagcat accaccaagc catgaatttg
     4861 ttgctaagac accgcttgca atgattggca cagtccatgg aacagagacg ccagttggtg
     4921 cagggacaag tccagcagcc attaccaaat aattgaacgt cgttacgatt agaggtgcca
     4981 caacccaagg aataaggatt gttgcattca atacgatcgg tagaccgaag atcgctggtt
     5041 catttacatt gaaaatacca ggtgctaaag ctaatcgtcc aacatcacga tactgtttct
     5101 ttttaagaac aaaggccatc aataaaacga cagctaaagt cattccagag ccaccaatac
     5161 caacagtgaa ggtttccatg aatggttttg tgatgatgtg tggtaattct tttccatgtt
     5221 gataagcttc taagttatca agcatcagtg tattccaaat cgggtccatt acagagttga
     5281 cgatgatttg tccgtgaagt ccgaagaacc ataagaattg gacaaagaac aaagcaatca
     5341 atgttgcagg taaactactt cccaaaccag ttaatggttt ttggatcact tcataaacga
     5401 cgtcatgtaa gttagcattg aaaataccag tcatcacagc attgattact aagaaaatag
     5461 ttagtgtcac tacagcaggg atcaaagcgg caaatgattt tgttactgca ggcggaactc
     5521 catcaggcat tttgatctgc cagccgcgtt tcgtgattct gcagtagatt tctccagcca
     5581 agaaagcagc gatcattcca atgaacattc cttttgcgcc aagacgatca agtgaaagca
     5641 caccagtcac atcttcaccg ccagcggttt tcattgcaaa aggcgtaaga atcaagaagc
     5701 tggcaaaaga cacggcacca ccgaaaatac cttcaacgtc ataagatttt gataaataat
     5761 agccgatacc aaaagtaaca aaaaccgaca tgatgctcat tgttgcattt tgcccattac
     5821 cgaataaatt cgataaagtt ccctttgttg cgtcactgaa aaaaggcaag ttattgataa
     5881 cgacaacgat cgaaccgaac atcgttaaag ggaaggaaag catgaaagca tcccgcagca
     5941 ctgtaaggta acgattttgc cctaataaat tagctaaggg catgattttc tcagaaagct
     6001 tgtccataaa accgttcatt tttattcacc tcaaatttat ttgcaatcga ttacatacaa
     6061 agtataacgc caaaaatttt aaatgtctag tcattttcat aaaaagtatt gatttatttt
     6121 ttagggttcg ttatgattac ggtatcaaga aaaaagagag gaagtaaaaa aatgaaaacc
     6181 tacacattcc cagaaaattt ttggtggggt tctgctgcta gcggaccgca aacagaagga
     6241 agagtaagtg atgatggtaa aggagaaaat atttgggatc attggtatga attagagcct
     6301 gaaaaattct tcgatcaagt aggacctgaa aaaacttcgc aagtttacac aaaatacaaa
     6361 gaagatgttc agttaatgaa agaaactggc cataacacat ttcgtacatc tatccaatgg
     6421 agtcatttga ttccagaagg gacaggtgaa atcaatcaaa aagctgtttc tttttatcga
     6481 gattatttta ctgaattgct ggataatggt attgaacctt ttgtgaacct ttaccatttc
     6541 gatatgccac taccactgca agaaaaaggc ggatggttat caagagaaac ggtcgacgct
     6601 tatgtttctt atgcagcagc gtgctttgaa ctgtttggag atctggtcaa aaaatggttt
     6661 actcataatg aaccaatcgt acctgtagaa ggaggctact tgtatcaatt ccactatcca
     6721 aatcatatca atatgaaaga agcggtccaa gtaggctatc atgagaatct agcaagcgca
     6781 aaggtaatca aagcctacca tgaaggcgga catgatggtg agatcggaat catcttgaat
     6841 ctgacaccaa gctatccgag agatgaaacg aacccagaag atgtaaaagc ggcgaagatc
     6901 gctgacgcat tcttcaaccg atcattcctt gaccctgcag tgaaaggaga gtttccacaa
     6961 gagttgatcg aaattgtcaa agaattggct ataatgccag agatccatga aggagaccta
     7021 gaactcatcc gtgaaaacac gattgacctg ctaggaatca attactatca gcctcgtcga
     7081 atcaaagcaa aagaaactcc aatcaatcac gaacatagcc ccatgccaga tgattatttt
     7141 gataattacg acatgcccaa taaaaaaatg aatccatatc gtggatggga aatttacgaa
     7201 aaaggaatct atgatatttt aacgaataca agagaaaact atggtaatat caaatgctat
     7261 atttctgaaa atgggatggg ggtcgaagga gaagaacgat ttgttaacag cgaaggaatt
     7321 attgaagatg attaccggat cgagtttgtt acggatcatt tgaaatacgt ccatcaagca
     7381 atccaagaag gaaccaattg tgtgggttac cacatgtgga catgtatgga caactggtct
     7441 tggacaaatg cttataagaa tcgctatggc tttatcgctg ttgatttggc acaagacggt
     7501 aaacggacga tcaaaaaatc aggtcgctgg tttaaaacgg tctctgaaaa aaacggattt
     7561 gatgcctgat attttttgat aaaacgttca caaaatgttt gttctaacac tgtaatcgtg
     7621 ctacactaac agtgaaggag gcgtttacaa tggtagatac ttatcgtaat attttagtag
     7681 caattgatgg ttctgaaaag gcagaaaaag catttcttga ggcaatcacg attgccaaac
     7741 gaaatcaggc aacgttgcac atcctgtatg tcaatgaagt aacaggcaat tactttggtg
     7801 attttgcatt tgttacaaca agccttcagg aagaattgga tgaggttgcg gaaaaccaga
     7861 tgaaagaaca tcgaaacttg gcaattgaaa aaggcctgac agatatcgag acatatgttc
     7921 tttacggtta tccaaagaca ctgattgcta atttccatga atcgaaagaa ccaatcgatc
     7981 tgattgtcat gggggcgaca ggtctgaatg cggtagaacg tgcactagta ggttccacta
     8041 cttcctatgt agtcaaccac gcatcatgta acgtcatggt tgtaaaataa ataaattaag
     8101 ggaacagaaa gcaaaaaagc ataacgcttc ttttgctttc tgttcctttt atttttttgt
     8161 aaaaaattac tttacctgat gaagtaatca tgctattcta attacaacga cagatggagt
     8221 aatatggagg aaagaaaatg atttcaagtg atggaatacg cggatacaat gatgcgatta
     8281 ttttatcgat ccttaacaaa aacgattctt atggatatga aatttctaaa acgattcgtg
     8341 agaggacaaa tgattcctat gtgatcaaag agacaacact ttactccgtt tttgcccgtt
     8401 tagaaaagaa tcaactgatt gtttcgtatc ctggagaagt gactcatgga aaaaagcgga
     8461 catattataa aatcactgaa atagggcgta atcttttaga agaaaaaaag caagagtggc
     8521 tgacgacgaa agacgtagtc gatcaatttt tggagggaaa ttaaatggat acaatccgta
     8581 attatttaga cagtcttttt atcggagtac ctcaaagtac cgagatagat aagctgaaaa
     8641 ctgatttgtt ggcaaatatg gaagaccatt atcatgaatt gatgggagaa ggaaaaaatg
     8701 aacaggaggc catcggtaca gtaatttcga catttggttc aatcgatgaa ttattagaag
     8761 aactagatgt cgaaaaaaaa catcaagctg acgagacgga aacaaacaca gcttctattt
     8821 atttaagcga agcagaaaac tactggaaag aatacagagc ggcaagtctc caagtggcat
     8881 caggtgttct attcattagc ttatcttttg caagttttct tttcttttgt tcagcaggat
     8941 atgtgttcat gggtatttct tgtctgattt tcggtatagc attagctgtc ggttttttta
     9001 ttgcttctgg tatgaaaatc acacgtttaa atcacttttt gcaccatcgg aaaatacctg
     9061 aaaaagtatt agcagaagca aaagaaaaag aagaagagta tcagcgaagt tttggcttct
     9121 cactgattgc aggaattgga ttatgtattt tttcactttt cccacttttg gcttctttaa
     9181 tgtggtatat ggatggtagt atcggtgcct ctatattttt tgttacggta ggaacgggtg
     9241 tcttcttgat catttatggc agtctagtcc gtcattctta tcgtcaattc actcaaagcg
     9301 cttattactg gtgaatgtat tcagatttgt aaaaatattc tatttatccg gcgtagaaaa
     9361 aaagactgtg acacgcttt
//
LOCUS       DACEMO010000105         9294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEMO010000105 DACEMO010000000
VERSION     DACEMO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.108"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..1257
                     /locus_tag="IW728_001837"
                     /pseudo
     CDS             1..1257
                     /locus_tag="IW728_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1364..2395)
                     /locus_tag="IW728_001838"
     CDS             complement(1364..2395)
                     /locus_tag="IW728_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025192.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(2402..3232)
                     /locus_tag="IW728_001839"
     CDS             complement(2402..3232)
                     /locus_tag="IW728_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1025193.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAKTGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(3229..4035)
                     /locus_tag="IW728_001840"
     CDS             complement(3229..4035)
                     /locus_tag="IW728_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1025194.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(4041..4865)
                     /locus_tag="IW728_001841"
     CDS             complement(4041..4865)
                     /locus_tag="IW728_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1025195.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(4855..5955)
                     /locus_tag="IW728_001842"
     CDS             complement(4855..5955)
                     /locus_tag="IW728_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1025196.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            6133..6918
                     /locus_tag="IW728_001843"
     CDS             6133..6918
                     /locus_tag="IW728_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1025197.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            6951..7334
                     /locus_tag="IW728_001844"
     CDS             6951..7334
                     /locus_tag="IW728_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1025198.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            7410..7541
                     /locus_tag="IW728_001845"
     CDS             7410..7541
                     /locus_tag="IW728_001845"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1025199.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            7510..7746
                     /locus_tag="IW728_001846"
     CDS             7510..7746
                     /locus_tag="IW728_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1025200.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(7824..8795)
                     /locus_tag="IW728_001847"
     CDS             complement(7824..8795)
                     /locus_tag="IW728_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1025201.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         2745 a         2142 c         1795 g         2612 t
ORIGIN      
        1 tatcaaacta ttactaggaa tcaaagatgg aaacattcaa atcactcatg tcgagaagaa
       61 aaaaatacat tcaatcaaca gtatcgttgt ttctgccacg ctcattaaaa cgattcagcc
      121 ctgtcctcag tgtaaacagg tgaatcaaca tgggctgatc gttaagaacg gaaagaaaaa
      181 atcgctggtc cttctcaata agtgtgcgaa tcaacggact tatcttgctt tggcaaagca
      241 acggtacctc tgtcgtggct gtcacaccta ttttacggcg aatacctaca ttgttgaaaa
      301 aaactgtttt atcgccaaac aagtacatta taaaatattg gaggaattga ctgaaaaaca
      361 agcaatgacg acgatttcta aacattgcgg cgtttcttgg tcaaccgttt cgcgaacact
      421 cgcttctctg ctccccatga caaacgtgaa aataaattgg ttgcctcgct gttcattggt
      481 agatgaattt cgttcattga aaaatcaagt cggtccctat tctttttctt gtatggatgg
      541 cgatactggg aaattactgg atattcttcc ttcacgtaag aagaaagatc tcatttctta
      601 ttttatgcaa tttgaacgac gtgcacgttt aaacgtaaaa atactcgtaa cagatatgaa
      661 cgcctcttat gctttgctga taaaagagtg ctttcctcat gccaaactcg ttgtggatcg
      721 ctttcacatc gtcaagcacc tcattcgcag ttttaaggat atttgactat gagtcatgaa
      781 aagcttcgac cgaaacgatc caatacaagc caaacactat cgacaagtga aggctttatc
      841 tcgacttctg atcaaacgac aggacacgct tgtttatgat aaatggacaa aatggcgtaa
      901 ttttggttgg gcctatttaa ccgaaagtga agtcgtagaa cgtctacttt ctacttctga
      961 tgaactacga atagcttatg cttactatca agaaattctt caagcttttt atgacaaaga
     1021 agcggacatc ttctttcagc ttgtaaaaac aatgcccaag tcggttccaa gagaactgca
     1081 tcatataaaa aaagcgttta taaactatga atccggcatt cgtttagcct tagagttgcc
     1141 ttactcaaat ggcaaaatag aaaatctgca tacccacatc aaagcattga aacgaatagc
     1201 ttacgggtta ggatgagaac cagaattttc ttaatcaatc atctgatcac ttattaaaca
     1261 taaaaaaaca tctaaagcag aaaatcaatt ctaccttaga tgttcttata tcactctatc
     1321 agtcctattt gacaaagagc cagaaatgtt tgccgactat acactagctt tgttgtgcaa
     1381 aatgctgagt atcaatttcc acagggattg ctagaatatc tttcagctga atgtataagg
     1441 catcgctaat tttctcttcc cgatcgttca accgatccac taaaatttct ttgccctcca
     1501 gcgtttgaaa aacataccgt tgaatgttgc ctaaaataaa tgattccttc catagaaccg
     1561 gaatcttaac cccctgagga accggttcaa attgaatcag ctccggtctt aaataataaa
     1621 tgcagcgatt gcccttagcg gaaaactcgt gggtatactg attcagctca taccattcga
     1681 aacgattata gttgccgata aattccgcaa caaatcggct ctgaggattc tgatacaatt
     1741 ccttcgaggt ggaaacctga gccacccgcc cagcttccat cacaaaaatc cgatcactga
     1801 ttcgcatcgc ctcttcttgg tcatgagtca caaaaatcat cgtcatccct agcgattgct
     1861 ggatagcgcg caaatcccgc tgcagctgtt ttctaattcg ggcatccaaa gcacttaatg
     1921 gctcatccag cagcaggact ttcggttcag tgaccaatga gcgagccagt gccacccgtt
     1981 gtcgttgacc gccagaaaga aactggacat ttttatctgc ctgctccgtc atatccacca
     2041 atgatagcat ctcaaaaacc ttttcctgaa tcacctcagg actcagcttc tttaccttca
     2101 aaccaaaggc tacattttca aaaacagtca tagttggaaa caatgcatac tgttgaaaaa
     2161 caaagccgat attgcgatcc ttcgtaggct tatcggcaat attctcattg tccagcacaa
     2221 tctcaccgct ctttggttgt tccaatcccg tgatacagcg cagcaacgta gactttccgc
     2281 acccgctggg acctagtaaa gtaaccagct cacccttttc aatctgaatt tcatcaatag
     2341 ctaaaatctg tttttcatca aaaaacagat tgagatcatg tatttctaaa aacattttcc
     2401 gtcacaactc ctttaagggc ttctttttcg actgcttctc ttccctcgtc tgcttaaggg
     2461 acagctgttg cttttgtcga taagtcagca taaaggtagt taaagcaatt cccaccataa
     2521 acagcaaata caaaaccatc acagcgctag ccaaatggcc gttttcgttc atccgccgca
     2581 gcatatagat gcgtaccgtc tgaaatcgac cgccaatcaa caggttcgtc agcatgtact
     2641 ccccaaaaac cgaagaaaaa atcatcagag aaactagggt cacaccgata cggatgtttg
     2701 gaaacagcac ctggataata atcgtagaca tcggggcgcc caaggtttct gctgcatcca
     2761 ctaattcctt cagattaatt aagcgcatct ggttgttaag actaagatag acaatcggca
     2821 acgccgtaat aaacaatgca cctaccaaca ctacaaacat tgggatacca gttttagcat
     2881 acgttcgaag caaagccgtt accaaaatca ctcctggtaa agcatacggc agcaggagca
     2941 gcttttccag ccaacgattc aaacgaggga aatacaaatg aatccaaata atggtgggaa
     3001 tcatcaacaa cagaatcgtc accaagacca cacctgctaa taaaagtgag cgacccaccg
     3061 cagcaataaa ttccctgtcc gtcagcagct gttggaacca ttgcaaggta aaatcactag
     3121 gtaaaagcga ttttgaccat ttagtggagc tggcatacaa aatcgttcca ataatcggca
     3181 gcaagtaata caggacaaag cagaacaaaa tcggttttat taactttttc acgtctgcac
     3241 caccttgcct ttcttttgaa taatcaacat tgccgttacc agcaggacac ctaaagcgac
     3301 tgccaacgca ctgcctaaat taggcttggc gaacacatct cccgacacta aagccgcaat
     3361 tcgaatcgtt aaaagattta cattactgct ggtcagggca tacgctgttt cataggtccc
     3421 catgccatta gcaaataaga tgacaaaggt gctggcaatc gatggtcgca aaaaaggcaa
     3481 agccaccttg aaccaagcat acgctgatga agcgcccagc aattgtgcgg actccagcca
     3541 ttcctttttc acttccttca tgctgggata caaaaacaag acccctaacg gaatttggaa
     3601 ataggtatac gtgatggtca atccccacca agaatacaaa ttgaagtccg ctagaaatgg
     3661 caaaatcatt ttcagcacac ctacattccc caacaaaata ataaaagaga atgccagcgg
     3721 aatcccagca aaattagctg ccaaattgga aacaaaggtg attttctcct gcaatttagt
     3781 cgatagattc attaagcata aacataaaat aaacgtcccg attaacccaa tcacactgga
     3841 gctgacagca atcagtacgc tgttaacaaa ggcctgtgtg taatatttgt tgctgaaaat
     3901 atcactgtaa ttggccaatg tccactggtt agttaaatca ctgtgcaagc tgcacagaac
     3961 cataaaaagc agcggcagca gtaaaaatgc acagaagata aaggaggcag gaaccagaac
     4021 caagcgtttt ttcatctctt tcatcccctc atcaattctt gaatgtcctg cattttctct
     4081 gacggtgcaa ttcctaataa ccagcaaaac aatggagccg ccagcagctg tggtacagtt
     4141 tgttccttca gcttttggcg tttatttgaa atcaaataaa aaggaacttc acgatgtgca
     4201 gccaagctgc cgccatgcag tccgtgcgca tccatcccgt gatccgcagt caccacgact
     4261 tgataacctt tttgcagcca ttcaggaagg taaatagcca ataatctgtc cgcctgatta
     4321 accgctgctg catattcatg agaatcggcg gtaaatttgt gaccgacatc atcaatattc
     4381 atcgaatgta cgaccaaaaa gtctggctga tactgattca ctaaataatg ggcatccgca
     4441 aataaatgcg aatcagggta agtgtcctca ctataaaaga tgccatgctg aatcagtcgc
     4501 tcctcctgat gctggatgcg atcaaccaaa cgattatagg gggcttgatt gtacaactcg
     4561 ctgtaccagt ggtaagcggc ggctcctgtt tttccgccag cttccttaac taattcaaac
     4621 agcgaccact ctttggatcg ctgagcatgg taattactat agatgccatt ttcataggct
     4681 ggaacgccgg tcatcagtac ttcgtataac ggacgtgaat tgctgggcag ctcacttttt
     4741 accttcaata agcttgcttg ctggtgctca actaaatgat tcatgaaccc aagctgggcg
     4801 acggctgtgt cgtagcgaca gccgtctagt ataactacaa tcagtttgtc aagtctactt
     4861 tgcataggtc agtacctccg cctgccaaag ctctggaagc tctacagagg tcttgttcca
     4921 agcttcattg tcttcaactg gcaccgccgc ttcataagca tcctccggca gtaatttttc
     4981 agctacttct ttaggcagct ctaccttgcg aataggtcgc gcatagccct ttgccaaatt
     5041 gatttgccct tcatcagata aaatatattc ccgtgctagc tttgccgcat tgggatgagg
     5101 ggcattttta ttgattaagg ttgtataccc gctaatgatg gagccgtctg ttggcacagt
     5161 tacctcaaaa cgctcggagt caatttggtc acgataattt agtccattga agtcccaaac
     5221 aactgccaca tcgatttcac ctttttcaag atttgctacg ctggcatcaa cggtggacaa
     5281 acggcctttt tctgccattt ttttgtagta attcagacct ggctgaatat ccttttcgtc
     5341 acctccgttg gcaatcgccg cagccaatac agcaaattgt gcttggttgg caatcgtcac
     5401 atcccccacg ctaacttgat agtcaccctc ttgcagctcc ttccagctag tcggtgcttg
     5461 cttcacattc tctttatcgg tgataaagga aatggttccc gtatagctta acaaccaatc
     5521 cccatcatca tcctttgccc aatccggaat ttcctcccaa tagttggtct tataaggtaa
     5581 ggttaactct ttgctttcag ccaacggacc gaagctgata ccgacatcac caatatcggc
     5641 ggttccattt tttccttctg attcaaactt agccaattct tctgcactgg acatatccgt
     5701 atccacgtga cttaagccgt attctttttc aaggtcgttc caagtgccta cccaatttgc
     5761 ccaagtatcc ggcattccca agctggcaat ctctccttct tccttggctt tttctataat
     5821 cgtatctaaa gagtcgtctg ccgcagccga ttgcttcgtt gcttcacctg agacgcagcc
     5881 tgtcagcagg atacttactg ttgctgcaca aaataaaccg atagtcatgt tacgaatttt
     5941 ttcattcatt ttcattcatt cttctcccct ttcgataatt ccattctaac aaagctttgt
     6001 aatgagagaa tcagtttctt gaaaaggttt catgaacaat gtaaaggttg tatatggttt
     6061 gtaaatttcg acgaattccc gttaaccttc taaagctttt gcggtatgat agtagaaaaa
     6121 aggaggctcc ctatgcagta ttatgatcag catctccaca cgtatttttc ctttgattca
     6181 gaggaaaaat ttgaaaacta tttagcctat cagccggagt ttttcgtttc tacggatcac
     6241 tttgatttaa acaatccatc taccggaaaa gatgacattc cggattatcc agcctatata
     6301 aacaaactgg actctttaaa agaaacctat tccaccaaat ttctgcgagg tatcgagatt
     6361 ggcgttgttc caggacaaga agagcggatc gtggattacc tcgctcagca tccttatgat
     6421 ttaaagctgg taagtattca tcaaaatggc cgctttgatt atatggacga tgtggtttta
     6481 accaaagatc ccattcaaac taccaaggaa tattttgatc agatgttgca ggtcttgacg
     6541 gattttccag aaggagatat tttgactcac tttgattatg gattacgccg cttcaccttc
     6601 acagcggaag aattcgcgga gcattttgag gctcagctgc taacgatttt caaaaaggtt
     6661 gttgaacgag aattggcgat ggagttaaat gccaagagct tcttgaaata cggcaacgaa
     6721 gacctttacc gttatgccgt tcctttgtat caaagtgtcg gcggaaagct ctttacctta
     6781 ggctcggatg ctcatgttgc cgaagattat cagctgggct ttaaaaaaat gagtcagctg
     6841 ttggcagact gtcaggtaga aaagttgcta gtcattcaag gaaaagaacg ccgcctgacg
     6901 ccgctgccga ttttgtgatt cctatataat agaagaaagt ggtgaaaaaa atgagcaacc
     6961 tgtgggacta caatcaagag gcacccatcc attatttaat tgcgcgacat tgggatgcgt
     7021 tgaaaattga ggcggtttgc cgatccttgt tagcagccgt tccaaaacaa caactggaaa
     7081 attttttggt ggctgacagc ttgcagcggg aaaaagttca ggcttatttt gcagccttta
     7141 aagatcagcc tttggaatat ttgcatgcgc agtttcatct tttttatcaa gtagccgccc
     7201 cagatgacta caacgattta cgaggacagc tgcaattgac ctttcaagcg gatgaaaccg
     7261 cctataccgt tttattggga atggctcgcc ttggtgatca ggcgaaggtc gagtggcgga
     7321 ttttcgatat ttgatggcag gatcaaggcg tttcagttgc aaaccttcct ctctttcaat
     7381 tagaaaaacc gcagcaggaa gaagtttgtg tgctggtcac cgatgctggt gaaattcgtc
     7441 tccgcttatt tcctgaccaa gctcccctgg ctgtagccaa ctggcgctcc ctagctgagc
     7501 aaggcgttta tgacaacaca cctttcggct cgtgtcatta aggaatttgt tattcaaggt
     7561 ggcgctttgg atggttctgg tgatgaagct gtttccagct atgagggctt tttcgctgat
     7621 gaagtgcatc gcggacttta tcattttaat ggcgccttgg ccttagggga ccacggcccc
     7681 cacaccaacg gcaaccagtt ttttattgtg caaaacacaa aggcgcaggc tgacctgcta
     7741 atgtaaagca agcatacttg gagcttggag gactaccgga gctggatggt cgttatactg
     7801 tttttggaca ggtataccaa gggttagaaa tcgtagcagc gatcgcagct cagccgacaa
     7861 acagtgaaga tgtaccgatt gagccagtgt tcattcgaca aattaacttc gaaaaaacat
     7921 cgtaaaaaga cactcactga cttcagcaaa agctctattc ttgagctggc tgcagctagt
     7981 gagtgtcttt catttatcca taagataaag cttggattca ataaataaat aaatgttttg
     8041 gttaaagctg tgaccagcaa atgaggaacc gtgcgagcca gttgatctgc caatgcaaac
     8101 atttctttag gtgaaggcgc tggcgtgtct gccaacggat actccagtcc caatgcttgg
     8161 taaccgtgca taccatagca gtggtacggt agtaattcta acggtacgaa ccgatcaaga
     8221 tctgccacaa aacaagcaat cgtcagcagc tcggtcaggt tcatattgat acctggaacc
     8281 acaggaactc gaattcgcac ttcgcagtta ggatactcaa ttaaaaagcg acgaatattc
     8341 ttcaaaattt gttggttatc taagccggtc cattgtttgt ggagcttgct ggtaaacatt
     8401 tttacatcca caaacaatgt atccacatag ggagctacct tttggatttc attataagca
     8461 ccacaaaagc tggtttccag tacggtattg attccaatgt atttcgattt ttgcaaaatt
     8521 tctttagcaa aatccgcctg aaccagtgcc tcgcctccgc taatggtcac tccgccatcc
     8581 gagtgaaagt aaaaaacagc atccttctca atctcatcca ttacctcttc agcggtcatg
     8641 gtctggccaa atcctttttc aatctgcttg ctttgtgatt cgggaatcga acaccaggca
     8701 cagcgtaatg ggcagccctt taaataaaca acggtccgta aaccagcacc atcatgtaga
     8761 gaaccctgtt ctattcttaa tactgttcct tccatataga tttttgaatt acacactagt
     8821 ttaaactaaa acacacaagg aaaaacttac catttaatca taaaattagc tcatataaat
     8881 atactaggat gtaaaataga ctggtcaaaa tctggtcaaa aaagaggact aatacaggta
     8941 gtcctctttt ttgttatttt aatattctaa atcatttttt attggtttat tcccttattc
     9001 gatgtagact caaatcacta tatcactaga tatcaaacgg cgtcatacgg aatcaaaaac
     9061 atcaaatttg acctgttaaa tggtcattga ataacattca aaatcaataa aacggagtca
     9121 gacacggagt caaaaacgct gagagtaaaa aatatataca gccttgtagg taaagtacca
     9181 cagggctgta tattttttat ttcagattac cttaaatgat tccatttact ttttatctca
     9241 ttttttccta ctagttagta tcaaatactc tcctattttc tcaataaact attt
//
LOCUS       DACEMO010000106         9213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEMO010000106 DACEMO010000000
VERSION     DACEMO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.109"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            52..789
                     /locus_tag="IW728_001848"
     CDS             52..789
                     /locus_tag="IW728_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1025202.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            817..1356
                     /locus_tag="IW728_001849"
     CDS             817..1356
                     /locus_tag="IW728_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1025203.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(1854..2315)
                     /locus_tag="IW728_001850"
     CDS             complement(1854..2315)
                     /locus_tag="IW728_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025204.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            2448..2822
                     /locus_tag="IW728_001851"
     CDS             2448..2822
                     /locus_tag="IW728_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ1025205.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            2876..5002
                     /locus_tag="IW728_001852"
     CDS             2876..5002
                     /locus_tag="IW728_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025206.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIF
                     VSFTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFM
                     IFLINLITSIIIYKKNSFKYFKENIL"
     gene            4999..5619
                     /locus_tag="IW728_001853"
     CDS             4999..5619
                     /locus_tag="IW728_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025207.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            6312..6692
                     /locus_tag="IW728_001854"
     CDS             6312..6692
                     /locus_tag="IW728_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1025208.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            6674..7588
                     /locus_tag="IW728_001855"
     CDS             6674..7588
                     /locus_tag="IW728_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1025209.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            7828..8518
                     /locus_tag="IW728_001856"
                     /pseudo
     CDS             7828..8518
                     /locus_tag="IW728_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8639..8725)
                     /locus_tag="IW728_001857"
     CDS             complement(8639..8725)
                     /locus_tag="IW728_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1025210.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
BASE COUNT         3564 a         1402 c         1423 g         2824 t
ORIGIN      
        1 ttttacatcc tcttatgtct atgataacat aatttattga ggtgattttt attgagtaac
       61 gatatagttc gatataataa tggattaaat tctgtccctt taagaaaatt tacccctata
      121 gatatggatt tattttgggg aatatgttct aaaatgaaaa gaaaaggtac aaaagaagaa
      181 atttttacat ttaaacaaat aaaagaatta attgattaca atataaatgc acgtggcgac
      241 cgttttgcta atgatttaat aaaaatgtca aaaaaaatca ttactttgtc ttttatttat
      301 gaagacgaaa aaacatatga acaattaaat ctgtttcaaa aatttaaaat cgataaagtg
      361 aatgaaacat tattaataaa ggtttctgaa gaatttgaat atatacttaa ttcaatcgga
      421 actaatttta ctcgtttcga attagaaaat atgacaaact taaagagtag ctacgttaaa
      481 gagttatatc gccaacttat ggctcataaa gacagaagat ctaaaaaagg tcactggttt
      541 gtaaaaatag aagattttag agctctatta gcaataccag aaagttatcg catgagagat
      601 attgataaac aaatttttgc ccaagcgaaa aaagaatttt taaacgttaa ttcaactcac
      661 cctgctattt ttaaaacttt tgaaatcaaa aaaattaaag cctacaaagg aaataagata
      721 ggatctctgc aaatttattt tgaagaaaat ataccttcaa ttaccttaga taattggtta
      781 gagaaataat acctaacaat ttaggaggca attatcatgc aacccaaaga aacctttaca
      841 ataaaggaat tatctgaatt atttgaaata agtaggcaag caatctctaa acatgttgat
      901 aaattagacc cgtccttatt agctaaaaat gagcgtggtt ataaggttgt attacgttct
      961 ggagtattgc aacttgctag caacttaggt aaccaaaggt tactatatag gttgcatgag
     1021 tatgaacaag aaaacgataa aaacgtttat atatcaagca aaaacgtgat tgtaaacgag
     1081 ttgctagata agttgcgtga agaaaatgaa tttttacgag aacaactttc cctcaaagag
     1141 caaaataatt ctaatatgca aaaattactc gaccagcaac aacagctcac cctacaggca
     1201 aatcaacaga tcgaaaaact gcaagaacag ttacaattag cttatacgga agaaactcca
     1261 gaagatgaat caactactat tctttctgaa gaaacagaat cgatagaaaa acacacaaaa
     1321 aagaagaagc aaaaatggtg gcaactttgg aaataactgg agggaacatt catgacaaga
     1381 aaaaatcttc tattcggttc tattttacta ggaattctag gcttcagtcc gatgttattg
     1441 tcagtaatca ataaagggaa taaacatggt gaattgaaat tgaatccaaa ttattacaac
     1501 agtcctaacg aagaattgtt tgtaaaagag acggataaat aataaaaaag actgctcttc
     1561 cgagcagcca gttaaaaaat aatgagtgtt tattcttgaa aaagcacgcg cttatgaagc
     1621 gctaccaaaa atttaccata ttttacaaaa aataacaagt cttaaaaaaa tgacagataa
     1681 aaatgcacaa agaattagat atagataacc gcgaaaaatc aagctttaga aagttgcttt
     1741 tagctagacc aattaaaaat cacaaagtga gcgtctctat ccattggtag aggggctact
     1801 tggcatagtg tacattatgt caactatgca attaaataac taactacttc atattagctt
     1861 aataaaataa tcaaaataac tagggggaaa aacattgtat aaaaaaattt ttcatttttt
     1921 aaataagaat agtaaatata aaaataattt aagatatcta ttactattaa tccagataga
     1981 gcaaaccaag taaatattcc atgtgtaaag ctaaaaaaat tcatttgaat caattcaaat
     2041 aaacctaata tagtaacaaa tatattaaaa ataatagctg tcaataccag taaattattt
     2101 tttttatttt ttgaattatc ttcatttaaa atagaattta tgttagtact ataaaattca
     2161 caaatttttt ttagtgtctc tatatctggt gttgttttgt tattttccca ctttgaaata
     2221 gtttgacgtg taacgttaaa ttttcttgcc acatattctt gagtataccc ttttttcact
     2281 cttaattctc ttagcttaga tcctaaatcc tccatttttt ctcctatgta aacgattatt
     2341 tacataaaat tttatcataa aaatactaac gcgccaaaaa agttctttat tttataaaac
     2401 acactaaaat aaaataattc tagtaaaaaa gataggagaa ataagttatg aaaaaatttt
     2461 tatgtctttc tgctttaatg ggtgtcttat taactagtgg aggaatagct agtgccacag
     2521 aagctttaaa tctagatgta cccgaagacc ataccgccat ttacggagga ggaatggaag
     2581 ctacagaaga aggattttct tctagtaaat tgagatatgc tgctggcggc ggggatttca
     2641 actgtggggt taatggattt aaagtttatg caaattacta ccatgctaga gctaaacata
     2701 gtgcaacagc taaaaatgga cgaggcggtc aagtacgatc agttcagaaa gctggagtaa
     2761 gagcttatgc aacatgcaat gccacactta ctggaaatac tggttggtgg aatgtttatt
     2821 aaaaaagaga gtttaaactc tcttttttat ttacatttga aaggaaattc aaaatatgtt
     2881 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat ttttttctac
     2941 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
     3001 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
     3061 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
     3121 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
     3181 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
     3241 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
     3301 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
     3361 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
     3421 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
     3481 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
     3541 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
     3601 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
     3661 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
     3721 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
     3781 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
     3841 aaatacccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     3901 atacaaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     3961 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     4021 cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta ttggacaaaa
     4081 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     4141 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     4201 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     4261 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg actctcctcc
     4321 tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     4381 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     4441 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     4501 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     4561 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     4621 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     4681 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     4741 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     4801 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     4861 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     4921 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     4981 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     5041 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     5101 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     5161 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     5221 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     5281 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     5341 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     5401 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     5461 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     5521 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     5581 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     5641 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     5701 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     5761 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     5821 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     5881 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     5941 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     6001 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     6061 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     6121 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcactaa
     6181 atacgtaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     6241 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     6301 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     6361 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     6421 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     6481 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     6541 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     6601 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     6661 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     6721 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     6781 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     6841 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     6901 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     6961 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     7021 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     7081 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     7141 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     7201 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     7261 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     7321 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     7381 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     7441 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     7501 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     7561 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     7621 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     7681 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     7741 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     7801 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     7861 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     7921 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt tttccttgat
     7981 aatcgtttca agcggcttat tcggcgtttt agagggaaat aagcgcgttt ctagaagtat
     8041 tttattagga aatgtcactc aaaagtatga atcagcgcgt gataaaaaga aaaaagagca
     8101 agcattagca caaaaaatgg aagagtttac cacttgttat gaggaaatga acgatatttt
     8161 tgtgaatcaa gaaaaacgtt tgacggataa aaacatggaa aaactcactc gtctttatca
     8221 aaacttgcca gaaaaacagc aaaaagaggt tcaagacaac tatgagcgaa ccaaaaaaga
     8281 cgtgcaatat gtaaaagata caaagataga agaagcctgt agtgatctat ttggagacac
     8341 aaacccttgg tttgccagtg aagaagaaaa aaaagaaaaa caacaatctg tcacatatga
     8401 acgctatgaa atcttacttc aacaagcaac taacatgcaa aatccgacaa aaaaagaaac
     8461 cgccttaaac tatctaaaaa gtgtcaagga atggttagat cagcaacaac aaaattgaat
     8521 ttgatttaaa atcatgttaa actgaatgtg caaatgatca aacattagat acacgaaagc
     8581 cccagagagg tcggattctc tggggctttt tttcaggttg atgtcgcctt tattgatttt
     8641 acttcttgtt tcggttattc aaccaataac tgaataaaga gacacaaact cctacaagaa
     8701 gggggcaaat gaacagttca aacattagat acacctcctt ccagtcgcaa gactgtcgga
     8761 taggcgacaa gttattatat cttaaaaaat taaatttgtg tggtgttttt aagtatcact
     8821 tgttttaggg ttgttagggc ttgccctgac cgcctgtaag gcgcttctcg actagattaa
     8881 gggaaattaa tcaagaattg tcttagaacg gcttagacaa gctttcagag tctcacacct
     8941 cctctttgaa caacggtttt agcaaaggag ccggaggcga ctgcgcagta taatatgaga
     9001 gcatagcgat cacacctttt tagcgaccgt agggagcaag ttttccagtt tagaaaaatt
     9061 cgcgaacctt tttggaggaa aaagtaggag ttttttaggc gggaaattca atgtgctctt
     9121 aagatatact gttagatata ctgttagata tactgttaat aataactgtt agggattagc
     9181 aaccttatgg gagagtaagt attgacatag gta
//
LOCUS       DACEMO010000107         9192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEMO010000107 DACEMO010000000
VERSION     DACEMO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.110"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            98..1615
                     /locus_tag="IW728_001858"
     CDS             98..1615
                     /locus_tag="IW728_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1025211.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1605..2876
                     /locus_tag="IW728_001859"
     CDS             1605..2876
                     /locus_tag="IW728_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1025212.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3051..3947
                     /locus_tag="IW728_001860"
     CDS             3051..3947
                     /locus_tag="IW728_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1025213.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3961..4824
                     /locus_tag="IW728_001861"
     CDS             3961..4824
                     /locus_tag="IW728_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1025214.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4830..5393
                     /locus_tag="IW728_001862"
     CDS             4830..5393
                     /locus_tag="IW728_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1025215.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5403..6926
                     /locus_tag="IW728_001863"
     CDS             5403..6926
                     /locus_tag="IW728_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1025216.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6938..7897
                     /locus_tag="IW728_001864"
     CDS             6938..7897
                     /locus_tag="IW728_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1025217.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            7983..9062
                     /locus_tag="IW728_001865"
     CDS             7983..9062
                     /locus_tag="IW728_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1025218.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
BASE COUNT         3004 a         1448 c         1938 g         2802 t
ORIGIN      
        1 ttaaaatcga taaaaaataa gtaagtttaa catttatatt attgacaacg ctttcaacga
       61 ggtgtattat tcatgtataa attaaaggag gtcagttatg gaagccacaa tgttgattag
      121 tagtgtcaag cgtgtaggca aagttgatcc gcggttgtac agttcattta ttgaacatat
      181 gggacgagca gtatatgaag ggatttatca gccagatcat ccatcagcta ataaaagtgg
      241 atttcgccaa gacgtcattc acttggtaaa agaattgaac attcccatga ttagatatcc
      301 tggtggaaat tttgtgtcag gatttcgttg ggaagacagt attgggccca tagcaaatag
      361 acctagaagg ttggatttag cttggcggac aattgaaacg aatgaagttg ggattcatga
      421 attttataac tggtgtcagc atgtgggggc cgaaatcaat atggctgtca atctgggtac
      481 tcgtggagta gatgcagctc gtaatttagt cgaatattgt aattttccta aaggaactta
      541 ttggagtgat ttaaggcgtt cgaatggtca agagagccct tttggaatta agacatggtg
      601 tttagggaat gaaatggacg gtccttggca gattggacat aaaacagcgg aagaatatgg
      661 acggttagca gaggaaacgg ctaaggcgat gaaactcgta gatgattcaa ttgaggtggt
      721 tttatgcgga agttcgaatc ggcaaatgcc tacatttggt gattgggagt taacagttct
      781 tgatcaagcg tacgatcaaa tagattattt gtctctgcat caatattatg gcaaccaaaa
      841 aaatgatctt tctcattatc ttgcacgttc cttagacatg gatcggttca tttctgaagt
      901 gacagcgatg tgcgatacag tcaaggcgaa gaaacatagt aagaagcaga tcaacctgtc
      961 ctttgatgaa tggaatattt ggtatcattc agcggaacaa gatgagcaag cagcgccttg
     1021 gcagattgct cctcctttac tagaagataa ctataatttt gaagatgcat tactattagg
     1081 ctgtctatta atcactttat tgaaacatgc agatagagta aagattgctt gtcttgctca
     1141 attggtaaat gttattgcac caatcaggac agaaacaaac ggagaagctt ggagacagac
     1201 gatctattat ccatttatgc aagctgctgt gtatggacaa ggagaggtgt taagcccaca
     1261 aattcgttca gcaaactatg cgacggaaga ttttgaagaa gtgccgtatt tagaaagtat
     1321 agccgtaata aatgacaaag aacttgtcat atttgcagtt aaccgtggag aagaagaaat
     1381 gaatctaact gttcacttgt ctgaattatc attagaggga gtgattgatt tttctgaaat
     1441 gagtggattt gatacaaaag caactaatct tcctaaaagt gaacaagtaa aaccaagtca
     1501 ttcaacaaat gtaaaagctg aagatgggaa aatcaattgt ttattgaaac cgctatcttg
     1561 gaatgtggtc agatgtcgtt tatcagatga aggaggaaca aaaaatgaaa ggtaaaaaat
     1621 ggttaattgg ctctttgttg gtagctgcaa caggtatttt aggagcttgc ggtggcggaa
     1681 gttcagcaga atctgattct gataaagaag tgactttgac tttttggaat gggtttactg
     1741 catctgatgg tgaaatattg caagaaatcg ttaatgaatt taataaaaca aatgataaaa
     1801 acatcaaaat tgaaatggat atcatgactt gggccaattt gaatgaaaaa cttcctacag
     1861 cgatttcttc taaaaaagca cctgacttta ttgcattaaa ttatccagat tttgcacagt
     1921 atgtagaaaa cggagcggta caaccattag acgatttctg ggaatatgat ggagtagata
     1981 aatctgattt taaagaaacg gctattgaat taggtgaagt agaaggtaaa cagtattttg
     2041 tccccatgca ggtacaaggc atgtatatgt attggaataa tgatttgttt aaacaagctg
     2101 gattagatac tgaacaacca cctactactt gggaagaatt gacagaaatg gcaccaaaat
     2161 taactgatag tagtaaaaat gtatcaggtt ttgtcttcaa taaggatgga actgcaccgt
     2221 tatataactg gatgttagca aatggcggta aattagtgaa cgatgattat actaagtctg
     2281 aatttgcgtc tcctgagaac cttaaaacgt taaaagcgat ccaaaaaatg atccatgtag
     2341 acaagactgg tccagaatcg atttctggcg ccgaaatgga caatttaatg aatgctggtc
     2401 aactagccat tgaaatcaat gggccatggt taaataacgg attgaaagca aacgagattg
     2461 attataatgt gactacctta ccacaaggga gtgattcaga caaaaaaaca gctattttag
     2521 atggcgtagg atatgccatt ccggcaagta cagataataa taaaaaagaa gctgtctatg
     2581 agtttttgaa atattggaat acaactgaaa ttggtaaaaa atggagtatc gaaaatggtt
     2641 ttcctgccta cttggattca gtagcaaaag atgaagaagt aaaaaatgat cctattgtat
     2701 ctgaattatc gaaacaaatg gactatgcag agccatattt accaggtttt acccaaatct
     2761 ctgcgatcaa taacgatatc atcaatccac tgattgaaaa attattagca ggagaagatc
     2821 ctgagacact catgaacgat gcagatgatg ccatcaatca attactgcaa aaatagtaca
     2881 gaactttata caggatagac tgttggcaag atacagatat ctgaatgatc gacctagtat
     2941 ttgcagatag gtgttattgc aaaaatcaca gcgattgttc ggatatctta ttcagattcg
     3001 tttaaaccaa gattgtatat tttatcaaaa tgaaagatga ggtggactat atgaacggat
     3061 tcaccaaata ttggaatcgt cctgatcgtg ctgcgtattt gtttttaact ccatcttttt
     3121 tagttttatt attatttact gtcataccgc ttttaggaac atttgctatt agttttacag
     3181 atttgaatat tttctttaca tcaagaaatt ttgtaagttt tgaaaatttc acacgtatat
     3241 ttagtgatga acgagcagtt aattcgttga ttcattcttt atatttcacc gtactcgaaa
     3301 cgccaacaca aattatcgtt ggtttattag cagcagtagc attatctaaa aataccagat
     3361 tcaatcgttt ttgtcgttcg acattttaca ttccagttat ttgttcattg acgtcaattg
     3421 cgattatttt ttctatgttg cttgatccga atgtaggggc gattccttac ttgttttcac
     3481 aagtcggctt gccgatccct caattttttc gcgatccaac ccttgctatg ccgacggtag
     3541 ccatcatgac tgtttggaag aattttggtg ttactatgac gattctcttg actgctattc
     3601 agggaatctc tccctctttg tatgaatctg ctcaaatgga tggtgcaaca aatcgacagc
     3661 agttttttaa cattacgcta cctcagatcg ttccctcctt aggtttttgt attttaacga
     3721 atctgattgg ttcgatgcaa gtgtttgacc aagtatatgt tgcaaccgct ggtggcccac
     3781 aatttaagac agaaacagct gttcaatata tttacagtcg aggatttaca gcaccttatg
     3841 aattgggcta tgcatcagca ctgtcttcgg ttttgtttat cattgttgca actatagcta
     3901 tctccactaa tgtgtacatg tcacggaaag aaaaacaaat gaaataggag ggaaacctgt
     3961 atggcaccag aaagctcgat tacagttact gtgaatagtc gaaaagcagt tagtggtaaa
     4021 aaaatcattc gaacgattag tatgctagtt atattgttgg ttattgcgtt tccttttttg
     4081 tggctgatta tttcttcttt taaacacgaa aaagacatta tctcatttcc gcctagaata
     4141 tttgcagata gctataccct agataactat attaaggttt ggacaacgat ccctttatta
     4201 gattacatca aaaatacagt gatttttgct ggagggacag tgatcacttc tgtattcttt
     4261 gattctcttg cggggtatgc atttgctcgt atgcgtttca aaggaaaatc agttttgttc
     4321 tatttcgttt tgctgacaat gatgatccca ttccaagtct tcatgattcc tctatttatt
     4381 gaagtcaatt tgcttggcat gcttgatact tatgcaggtt tgatcattcc tcgtatgaca
     4441 actgcatttg gtatcttcat gatgcgttcc ttttttatta ctttaccaga ttctttggaa
     4501 gaggcagcac gcattgatgg attaagtgag tttaatattt ttttgaaaat catgttgcca
     4561 ttgagcaaac ctactctttt atcattaggg attttcacgc tgatgaatag ttggaatgac
     4621 ttgctttatc cattgatatt gacaagtagt tcaaaaatga gaactttacc agcagggtta
     4681 gctttattta cagggcagaa catttctttt tatggacctg taatggctgg aacggttatt
     4741 tcaatgctgc cattactagt ggtttatatt tttgcacaga aatattttgt tcaaggaaca
     4801 gctatgtctg gaatgaaaga gtgaggaaga tgaaaatagt tgagactaaa ctttatggac
     4861 agttgattgt ggtttctgat tacatcttat taggtattct ctgggtaatc gtgtgtttgc
     4921 ctgttattac tatcgtgcct gctacatgtg ctgtttttta tgtaatggcg caatggaaag
     4981 gtgagggatc aggaaatatc atttattttt tctttcaagg aataaagcaa cattttggta
     5041 agaacttgtt ccttagtctg ttaacgatca ttatttattt tcttgttaat caattattag
     5101 aagaacagac aatcgtactt tcaattagtg gatatgtagt tttacttatt tattccatgt
     5161 ttcttttatc ctggatatat gtgatttctg aaaacaaaga tatcacattt ttacaaattt
     5221 ttgaacaaag tgcatggaaa gtattttttg attttttgag aaatattctt tgttgcgtat
     5281 tgttattagg atttgtactg ctcatatttc ttttccctcc ttttattttt atctttgctg
     5341 gtggcgtatg gaaacttgta tctattatct tgactatagg aaatgaaagg tagggaaagc
     5401 tgttggacga agaagtgaaa gtaaaaatca gagaggattt tacggtagga gagatcagtt
     5461 ctcggctgtt tggctcattt gttgagcata tgggaagtgt cgtctataca gggatttatg
     5521 aaccgggcca tgaaacagct gatggtgacg ggtttagaca agatgtcctc tcttatgtaa
     5581 aggaattaga attaggtgtc attcgctatc ctggaggaaa ctttacatct ggttatgatt
     5641 gggtggatac tgtaggtagg aaagaacaac gaccaaaaaa aattgattta gcatggcgag
     5701 gtatagaacc aaattctttt ggactccacg aattttttaa atggatcagt catgtacaag
     5761 cgaaaccgat attcacggtg aatttaggaa caaaaggaat tgatgaagca aaaaatttag
     5821 tagaatattg taattttcca aaaggaacct tctggagtga tttaagaata aaaaatgggc
     5881 aaaaaaagcc ttttggcgtt aaattatggt gtttagggaa tgagctagac ggtccatggc
     5941 aagtgggggc taagagtgct tatgaatatg gtaggttagc aaatgaagcc tcaaaagtca
     6001 tgaagttagt ggatgattca attgaaacaa tcttagttgg aagctctacg ccacgtttac
     6061 catcgtaccc tgaatgggat cgtatcgcat tagaagaagc ttatgagaat gtagattatt
     6121 tagctttgca taactatatt gataagtatg atgaagaaga cttgactaaa cctcctaaaa
     6181 atgagcgtga tgatacagcg acataccttg caaaagctta tcgattcgat cgtcaaattg
     6241 aagaaatcgt tgctacttgt gattatgtaa aaggatattt gagaagtgag aaaacaatgt
     6301 atctagcgtt tgatgaatgg aatgtacatg cccaaccgga aaaaagacat agggattttg
     6361 aagtcggtag tcccatcgat tggtgttact ttactatgga agatacatta ttatttggtt
     6421 cattagggct ggctatttta cgacacgcag atcgcgtaaa gattagttgt caatcattgt
     6481 tagtaaacac aataccttta atactgacag atgctaaagg acaagcatgg aggaatccaa
     6541 cttattatgt gatgcaacac ttatcgaaat atgctaaagg tcaagtattg aaacaacaaa
     6601 tcatttgccc tgtttatgat tgtgaaaaga taaaaaatgt tccttatgtg gacagtgtga
     6661 tcgtagataa cggcaatcag ctgactattt tcttgatcaa tcgcacaaat gaacaacaaa
     6721 agataacgat tgaacatgat ttcaatttgc tagatcaagg agagcactac acattaacca
     6781 gtagcgatct gttggacaaa aatacacgtg aagaaccaga aaaaataaaa cctgaaataa
     6841 actatttttc aatagaaaaa catgacgaat taaatgtagg gatagaaaaa tactcttgga
     6901 atgtaattag aataaagaaa atgtgaggaa atttgttatg atgaagacac tttataataa
     6961 tcctatcgtt cggcaacggg cagatccttg ggtatataaa catacagatg gttattatta
     7021 ttttacagga tcggttccag gctaccaagt gattgaatta agaagggcaa agtcactgaa
     7081 tgaattagaa catgctgcaa gcctgattgt atggcaggct catgaagaag gaccgatgag
     7141 tgaattgatc tgggcacctg aagtccatta tatcaatgga aaatggtata tttactttgc
     7201 tgcaagcaat cataaaacta tacgtgatga atctcatcat caccgcatgt tcgtacttga
     7261 aaatcagcat atagatccaa tgaatacaga ttggatagaa aagggacaaa taaaaacgaa
     7321 aagggaaact ttcagtttag acggaacaac tttctcttct caaggaaagc tatattatgt
     7381 gtgggcccaa caagatcctg aaattactgg gaattctaat ctttatctat ccgaaatgga
     7441 taatccttgg acgttaaagg ggaagcagat cctcctttct atacctgagt atgaatggga
     7501 aaaacggggt ttttcagtaa atgaaggtcc agcagtcctt actcgcaatg ggaaaatatt
     7561 cattacgtat tctggtagtg caacagatga aaactatgcc atgggcctgc tgtgggctga
     7621 tgagcacaag aatcttttat taaaagaatc atggcataaa ttatcggagc cagtattcgt
     7681 ttcttctgaa aaaaataagc aatttggtcc tggtcacaac tcgtttacgg tttcagaaga
     7741 cgggtgcttt gatattttga tctatcatgc tcgaccagag aagaaccaaa aaggggatcc
     7801 gttagacaat cctaatcggc acgcgaacgc gcaatgcttt acgtggaatg agcaaggatt
     7861 tccagaattt ggtgaacctg ttccgtatgc cttatgagca caagaaacct tgatttttgc
     7921 ttgaataaac cttgcagact cgttatgata tatgtacgta caaataaaaa ggggcaaggc
     7981 aaatggcaaa atatcaagat attgcagaag agataaaaca aaagatcctt cgtgaggaat
     8041 acgaagtaaa tacaaccatt ccctcagaat tcaagctgca agaaatatat gacgtcagca
     8101 gacatacgat tcggcaagca atcgctgtgt tggtaaatga gggctatctg cgtaaagaaa
     8161 aagggtcggg cacatatgta gatgacagct ataagaaaga aaaaacacca ttgaacagaa
     8221 caaaaacgat cggtgtcatc accacatatc tctctgatta tatttttccg tcgattatcc
     8281 gaggaatcga aaatgaatta cgagaatctg ggtattcatt gcttttagcc agtacgaata
     8341 acgatcacga attggagaaa gagtgtttga agaaaatgat tgcttatggg gtagatggtt
     8401 tgatcgttga gcctacgaaa agtaatcagt ataacccaaa cttagcactt tatgttgctt
     8461 tgagagaaca aggcattccg atggtgatga tcaatgctgc ttatgaagag ttaaatgcct
     8521 cttatatttg tttagatgat gtgaaagtcg gttttatggc agcagaatat ttgataaaaa
     8581 aaggacatca gcgtctgttg ttcatcacaa aaatcgacga tcttcaagga aaatacagga
     8641 tgaaaggttt tatcagcgcg tgtgaacagt ataatgtgcg ttttttgacg gaagatatca
     8701 ttactttcac cacggaaaca cgagaaacaa tcgctgaaaa aacagtacaa cggttgaaag
     8761 aagaacaagc tttagatggc atcatttgct ataacgatca aatagctagt ttaatgctag
     8821 ataagttgac agaaaatggc tatcatattc cagatgacct atcgatcgta ggaacagacg
     8881 attcttcttt aagccattta gggaataaaa aattgacaac attgacgcat ccaaaagaaa
     8941 agttgggaca agatgccgca cggtggatcg tcacaacgat tgaaaaagga caggcacagc
     9001 caaatattct ctatgaacct gtactagttg aacgggaaac agtgaaagaa aacgaaaaat
     9061 aagagacggg tttttaccgc ctcttattgc tcctacggtt actcgtcgca atgtttgtga
     9121 gagcttttgt catcgctcaa ggctgaacgt ttctttcacc acctctccac taaacggtgt
     9181 tctcctacca ac
//
LOCUS       DACEMO010000108         8884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEMO010000108 DACEMO010000000
VERSION     DACEMO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.111"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(263..1630)
                     /gene="dnaB"
                     /locus_tag="IW728_001866"
     CDS             complement(263..1630)
                     /gene="dnaB"
                     /locus_tag="IW728_001866"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1025219.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            complement(1904..2356)
                     /gene="rplI"
                     /locus_tag="IW728_001867"
     CDS             complement(1904..2356)
                     /gene="rplI"
                     /locus_tag="IW728_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1025220.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            complement(2362..4335)
                     /locus_tag="IW728_001868"
     CDS             complement(2362..4335)
                     /locus_tag="IW728_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1025221.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            complement(4478..4714)
                     /gene="rpsR"
                     /locus_tag="IW728_001869"
     CDS             complement(4478..4714)
                     /gene="rpsR"
                     /locus_tag="IW728_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1025222.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            complement(4740..5252)
                     /gene="ssb"
                     /locus_tag="IW728_001870"
     CDS             complement(4740..5252)
                     /gene="ssb"
                     /locus_tag="IW728_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1025223.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            complement(5302..5601)
                     /locus_tag="IW728_001871"
     CDS             complement(5302..5601)
                     /locus_tag="IW728_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1025224.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            complement(5837..8308)
                     /gene="gyrA"
                     /locus_tag="IW728_001872"
     CDS             complement(5837..8308)
                     /gene="gyrA"
                     /locus_tag="IW728_001872"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1025225.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            complement(8328..>8884)
                     /locus_tag="IW728_001873"
     CDS             complement(8328..>8884)
                     /locus_tag="IW728_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1025226.1"
                     /translation="KASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
BASE COUNT         2343 a         1976 c         1610 g         2955 t
ORIGIN      
        1 agaggtcgta agatcagcgt tttgacctga gtattagaga aacaaacgtc aaaatagttc
       61 ctcatatttt gacgtttgct gagctaatac caagggtcag ctgattgaac accgtttatt
      121 cggaaaaagg gactgtgaca agacttttgt cacaatccct ttggagatct gtttaaatta
      181 agtactctag tcgttcctgc ggttactcct tgcatcttct tacgagagct ctcgggattg
      241 ctcaaggctc acaacctctt ctttaaaatt cttctcttgg cgaaagcgac gaaaacttat
      301 tgtattcttt gataaacagt agttcaactg ttcctcgtgc accgcttcgg ttcttctcga
      361 tgatgacttc gatgacgttg tcattttttg gctcgtttcc atcatcgtca tctccgcctt
      421 ctctttcata ataatcatct cgatacaaga aggctacgat gtccgcatct tgttcaattg
      481 atccggattc acgaatatca cttagcactg gtcgtttgtc ttgtcgctgt tctactccac
      541 gagataactg tgaaagagca atgacaggta ctttcagctc tttcgccagt tttttcagtt
      601 ggcgagagat atctgatact tcttgttgac ggttctcttt accgttacct tcaatcaact
      661 gcaaataatc gatcaatatc aagcctagat tgcctttttc ttgcgctagt ttccgacatt
      721 tcgcacggat ttcagagatt ttgatccctg gtgtatcatc gataaaaata tttgctcgag
      781 aaagactccc catcgcaacg atcagattcg accattcttc ttccgaaagc tgacctgtac
      841 gtaaatggct ggcctcaatc gaaccttctg cacaaagcat ccgatttaca agagattctg
      901 cccccatttc caaactaaat atcgcaacag aacgatctgt ttttgttcct acattttgag
      961 caatattcaa tgcaaacgct gttttaccta ccgctggacg tgcagcaaga atgattagtt
     1021 cttcgggctg gaaacctgct gtcattttat ccagaccttg atagccagtt ggtagacctg
     1081 tgatttcttc attgttttga gctagtcgat cgatattttc aatcgtggag ttcagtactt
     1141 cagcaataga taaaaagccg cttcgatttc ttttttccga aacctccaag atactgcgtt
     1201 cggcatcatc taaaattgtt tgaacttctt ctccttgctc gaatcctttc gtaacgattc
     1261 ctgttgctgt ctggatcaat gtacgcagca atgatttttc ttcaacgatt ttcgcatagt
     1321 agacgatatt tgctgctgtc ggaactgcta atgccaagtc tgagagataa cttaaaccac
     1381 cggcatcttc taacaaattg gcttgttcca gccggtcttt gacagtaacc acgtcaatcg
     1441 cttcattacg atcatttaac tcgatcattg tctcaaaaat aatttgatgg ccacgacgat
     1501 aaaagtcttt tggctctatg tactccatgg catcaatgat cgtttctgcg tcaagaaaaa
     1561 cagagcctaa aaccgcttgt tcagcttcaa tattttgcgg tgggattcga tcctgccaca
     1621 cttcattcat acgtatcgct ccttgtcttc aattgcttca ttttacaaga aaatgagaga
     1681 aaagtcatca ttcgtaccga aagtgtaata aaaaaatagt tctcttttat tatactcgtt
     1741 ttctttatgc tattttgctg ttcccaaaaa agaaaccgaa atcaatctga aatcgatttg
     1801 cagcttttgc tttcattgct gcagctgttt tctcagataa atttcggttt gtcttttttc
     1861 cttctggtta tacttaccag ctgaacatcg ctatcaagat ttgttattct tgtacgacat
     1921 gaactttgat cgtagccgtc acctcatgat gaagcttaac aggtacgttt gtgtagccta
     1981 gtgaacggat cggattttct aattcgattt ttcgtttatc taatttgatt ttgtattgtt
     2041 tgtttaaccc ttcagcaatt tgttttgaag ggatcgaacc aaacaaacgg ctgtcttccc
     2101 ctgctttcgc cttcaattcg actacagtat cttctttttc caagaattct tttaactttt
     2161 tagcttcttc caagatttca gcttcgtgtt tttcttgtgc tttcttttgt cctgctaact
     2221 gactgatact gcttttcgtt gcttcttttg ccaagttatt tttgattaga taattttgtg
     2281 cataacctgc tggaacttct ttgatgtccc cttttttacc ttttcctttt acatcttgta
     2341 aaaaaattac tttcatttgg attactcctt atcatatatt tgattcatat tttctttgat
     2401 tgcagcaaat agcttctgtt ttgtttcctc aactgtcgta ttttccagtt gagtagctgc
     2461 gtttgtgaag tgtcctccgc cacctaagga ttccatgatg atttggacgt tgatcgtgcc
     2521 ggtacttctg gcactgatcc caatcaagcc gtctgtccgc tttgtaatga cgaaagctgc
     2581 attgatattg atcatcgaaa gcaatgtatc tgctgttttt gctgctataa cactattata
     2641 ctctttttcg tctgtccctg ccgccacaac aatatcagaa gtcacatagt cgcttccagc
     2701 aatcaaattg ctgatctcca agtatgtcgc taaatccgag ctcaataaat attgtactaa
     2761 ggatgcgtct gctccacaag accttaaata gctggcaacg tcaaaagttc gtgcagttgt
     2821 acggacattg aaactatttg tatctacaac catccctgca agcaataatg tggcctcaaa
     2881 acgatctaag cgatttgttt tgctgctttg gtattggatc agctcagaaa ctagctctga
     2941 agctgaagaa gctgaagatt caatataagt cagcagaggt ttacttggaa actcttcccc
     3001 acgacgatgg tgatcaatga tcacaatctt atcaaattgc tcatataggc cctgagagat
     3061 cgatagagaa ggtttatggt aatccaccat cacgaggagg tcctcttctt ttttcagctc
     3121 tatggcacgt tctggagaga ttagttgcat ttctaattca ggatgcttat ggatttcttc
     3181 aagacaccgt tctacatctg gaatgatctc gttctcattg ataacgatgt aagctttttt
     3241 ctggttgaag cgtgccagac aactgacacc aaaagcagag cctaaagcat ccatatccgg
     3301 aaacttgtgc cccatgataa acacatcgcc tgtttcatca aaaatccgac gcaaagcggt
     3361 actcatcgct cgagaacgaa cacgcgtgcg tttgacattc ttagcagatt tccccccata
     3421 ataaacaggt tttgctcctt cttttacctc tttcacgaca acttggtcgc ctccacggac
     3481 taaagcaaca tctaaattat tctgagcttc tccaccgatt tcatcgaggg ttcttgagcc
     3541 ataagcaatc cccatactaa gggtgatagg cgagctctgt ttttcagctt ctgctcgaaa
     3601 gcgatccagc aaatcaaatt ttttatccat catctttttc agatcgatca tctgtgctac
     3661 gaagaaaaac cgttctgcgt tgattctttt gaaaaagaca tggtattcat tcatccagtc
     3721 agaaactagt gtagtgacga aagagttcaa ataagaaatc tctttgtcat ctaatttatc
     3781 aatggcatct gcatagttat caatagataa aataccaatc accggctgac tgttcgtctg
     3841 atggagtttt aacaactggt tttgggtaat atcattaaaa taaacaacac cctttactgt
     3901 atccatatca aactgaaatg ttttatctgc tagatagact tggtttttct ttttttcttt
     3961 tgcagtaaaa aaagtatcta gcaattcttc ctctgttaat ccactttgat ctttgatttt
     4021 caatgctaat ggattgagcc attcgatttt ttggcttgat ggctgataag caatcactcc
     4081 tacaggcatg acttctgaga cataagctag attggtttca gctacagtcg aagcctgctg
     4141 gattttttca cgtttattta aaataagcca acgtttcatt ttcaaccaat agcgaacacc
     4201 ccataaattc atcaatagaa tgaccgaagc gctaacaaaa cgaaatggta aaattaagac
     4261 taccaatact tcgattaata taaacaggat aaggaagaga ggtttcgggg ataaatgatt
     4321 gtttttattt tccataattt tcctcccata tatgactaat ttgattttac cataactttc
     4381 gtcattcgag cgactaaacg aatagaggtt atttttacag aaaaaaagga attgaatagc
     4441 tgatcaaaag gatcagtcat tcaattccta ggatttatta gtcttcgcta acgaaaggta
     4501 gcaagcccat gatacgagcg cgtttgattg caacagtaat tttacgttgg tttttagcgc
     4561 ctgtaccagt tacacgacgt ggcaagattt taccgcgttc tgaaataaat ctttttagta
     4621 attcgatatc tttataatcg atatattcaa tatggttagc agcaatgtag tctactttac
     4681 gacgtttacg tccgcctctt ctttgttgtg ccattctatt tccctcctat caaattcgtt
     4741 tagaatggta aatcatcgtc tgaaatgtcg atcgttgaac ttgagccgaa tgggtcagaa
     4801 gtatcacggt cgaaatcagg cataccgttg gatgattgag atgtttgatt agagttattg
     4861 tttccgccaa atccgccagc tgattgacca gaactttgga atccgccgct ttgttggcga
     4921 ttttcagatg cagaacgact ttccagcaat tggaagttgt cagctactac ttctgtcacg
     4981 tatacacgtt gtccttgctg attatcataa gaacgagttt ggatacgtcc agtaacacct
     5041 aaaagtgttc ctttacgagc atagtttgct aaagtttctg ctgatttgcg ccaaatcaca
     5101 cagttgataa aatcagcctc gcgattgccg ctttggtttg taaaattacg attcacagcc
     5161 aaagtaaatg ttgcgactgc cgtaccactt gctgtgtaac gcaaatctgg atctttcgtc
     5221 agacgtccaa ctaatacaac attattaatc aaaatagtca tctcctctca tcgttttcta
     5281 ttgtttcaca tgaaacaaca attaagcttc ttctttaaca atcatgtgac gaatgatatc
     5341 gtcgttgatt ttagcaagac ggtcaaattc attgacagca tttgctgatg atggagaaga
     5401 tactttaacg atgtgataga taccttcacg gtaaccgttc atttcataag cgaaacggcg
     5461 tttttcccaa tcttttgatt cgataacttc tgctccgtta tctttcaaga ttgcatcgaa
     5521 acgttcgatt aaagcagttt ttgcttcttc atcaatgttt ggacgaataa tgtacatgat
     5581 ttcatatttc gtgttttcca ttgatattca cctccctgtg gactttcggc tcttaggttc
     5641 ctaagagcaa ggagagttat ctaattaatt agactactca caagctctga ttatacactc
     5701 tattttggtc aaaagcaata cagaatgaac agtttcgaat attttaagag aaattcaact
     5761 aatcaaaaaa gccagcagga ataaccagat tttttaatcg gtcattcttg ctagcttaca
     5821 caattagtct tgatggctat tcttctgatc cttgttcttc aactgtttgt tctgcagctt
     5881 ctggttcgac tgctgccatc gtgacaactt tcgtatcagc ttccatcttc atcaaacgta
     5941 cgcctaatgt tgaacgtcca gtttgagaaa cagtcgatac gttgaaacgg ataatgacac
     6001 ctttatctgt aatcagcatg atgtcttcat ctccagaaac agtggtcaat ccagctagtg
     6061 gaccattttt ttcagtgatg ttcgccgttt tgatcccttt acctccgcgg cctttcactg
     6121 gatattccga tacttttgtc cgttttccgt aacctttttc tgtgatcact aatacttctt
     6181 gatcttcctt cattaaggct gccccaacga cataatcttc ctcacgcagg cgtattcctc
     6241 taaccccgga agctgtacgt cccatatcgc ggactgcttc ttctttgaaa gtgacagagt
     6301 agccgttatg tgttccaata atgatattag attgaccatc tgtcagtagc acattgacga
     6361 gctcatcgtc ttctttcaaa ccaattgcga tcagaccatt acttcggatg ttagcaaaag
     6421 cagtgacaga agtacgtttt actgtacctt gacgcgtcgt aaagaagaga tagtggcctt
     6481 cttctgcttc tcccgtcacc gcaatgattg cctggatgct ttcactcgaa tcgataccta
     6541 gtaaattgat aattggtatt cctttggctg tgcgcccgta ttctggaatt tcgtatcctt
     6601 ttgcccgata cacttttccg ttattcgtaa agaataacaa tgtatcatgg gtagaacaag
     6661 aaaccagatt tttgacgaag tcgtcattgt ggatacccat tccttggaca ccacgtccac
     6721 cccttcgttg agctctgaat tcattatttg ccaaacgttt gatgtaacca ttgtttgtca
     6781 atgtaataac gatttcttct tcttcgatta aatcttcatc ttccagactt aagacctcac
     6841 cgactagtaa ttctgtgcgt cgtttatcac caaatcgttg attcaagtct gctaattcat
     6901 tcttgatgat ttctgtaaca cgttctggtc ttgccagaat gtcagttaaa tcttcaatca
     6961 gttttaagag ttcttggtat tcattttcga ttttttcccg ttccaaaccg gtcaatcggc
     7021 gtaaacgcat atctaaaata gcttgagact ggcgatcgga taaatcgaac cgttcgatca
     7081 tcgtcgcttt tgcttcgtca tctgatttag acccgcggat aatcgcaatg atttcatcga
     7141 tatgatccaa agcaatacgc aaaccttcta aaatgtgtgc ccttgcttct gctttttctt
     7201 tttcaaagac tgtccgtctt gtgatgactt cgcgctgatg ttcgatatag ttttcaagaa
     7261 tttgtttcaa gctcaaaact ttcggcacac ctttttcgat cgccaacata ttgaatccaa
     7321 atgaattttg caaggatgtc aatttatata agttattcaa gataacagat gcgctggcgt
     7381 cacgacggat gtcaatgacg atccgcatac cttcacggga agattcgtcc cgcaaatctg
     7441 tgatcccttc gatacgcttg tctctgtgca gttcagaaat ccgttcgatc aattttgctt
     7501 tattgaccat ataaggcaat tctgtcacga tgatccgttc tttaccattt ggcatctcag
     7561 taatctcaac ttttgcacgt acggtaatag aacccttacc tgtttcatac gctcttcgga
     7621 ttcctgattt tcccataacc aaaccacctg ttggaaaatc tggtccaggt aatacttcca
     7681 tcaattcatt ggtcgtcaca tcaggattat ccatcagcaa atcgatagcc gctactacct
     7741 cagacaagtt atgaggtggg atattcgtgg ccatacctac ggcgatcccc gttgttccgt
     7801 tcactaaaag attcgggaag cgagcaggta gtacaactgg ttctttttct gtatcatcat
     7861 agttgctttg gaaatcaaca gtgttcttat tgatatcacg cagcatttct gtagcgattt
     7921 tgctcatccg tgcttcggta taacgcatag cggcggcacc gtcaccgtca acagaaccaa
     7981 agtttccatg accatccact agcatataac ggtagctgaa tggctgtgcc atccgtacca
     8041 ttgactcata gatcgcatag tctccatgag gatggtattt acccatgaca tctccgacga
     8101 ttctggcaga ttttttatgg gctttatccg gtgttacacc taattcgttc atgccgtata
     8161 aaatacggcg atgaacgggt ttcagaccat ctctgacatc aggaagtgca cgtgcgacaa
     8221 taacactcat cgcataatcg atgaaggagt ctttcatttc gctggttaga ttgacatcat
     8281 ggatgttttc tctgatttct tcactcattc tttttcctcc ttcatgatta aatatctaag
     8341 ttcttcacat agtgtgcatt ttcctcgatg aatgcacgac ggggttctac tcgatccccc
     8401 atcaacattt caaatatctg atctgctgca atagcatcgt ccacactgac gcgggccatc
     8461 atccgattgc ttgggtccat agttgtttcc catagttgat gatcatccat ttctccaagt
     8521 cctttataac gctgaacgct tggttttggt gaggcaggaa gtgaagcaac gacttgtttc
     8581 aactcttctt ctgcattttt tcctggttgt acatacgtaa tattcttccc ttgttttacc
     8641 ccgtataaag gtggctgagc aatatagacg tatccagctt caacaatagg acgcatgtaa
     8701 cgataaaaaa gagtcagcag caatgtacgg atatgcgcgc cgtcgacatc ggcatcagtc
     8761 atgataacta atttgtggta gcgagctttc gatacatcaa aatcttctcc aaaccctgta
     8821 cccatagcag taaataaaga ccggatctct tcatttgcca agatcttgtc catgcttgct
     8881 tttt
//
LOCUS       DACEMO010000109         8867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEMO010000109 DACEMO010000000
VERSION     DACEMO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.112"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..695)
                     /locus_tag="IW728_001874"
     CDS             complement(27..695)
                     /locus_tag="IW728_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1025227.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            829..1116
                     /locus_tag="IW728_001875"
     CDS             829..1116
                     /locus_tag="IW728_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025228.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1532..3070
                     /locus_tag="IW728_001876"
     CDS             1532..3070
                     /locus_tag="IW728_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1025229.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3067..4032
                     /locus_tag="IW728_001877"
     CDS             3067..4032
                     /locus_tag="IW728_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025230.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4019..5341
                     /locus_tag="IW728_001878"
     CDS             4019..5341
                     /locus_tag="IW728_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1025231.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5404..5508
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5582..6325
                     /locus_tag="IW728_001879"
     CDS             5582..6325
                     /locus_tag="IW728_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1025232.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6340..7332
                     /locus_tag="IW728_001880"
     CDS             6340..7332
                     /locus_tag="IW728_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1025233.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7344..8081
                     /locus_tag="IW728_001881"
     CDS             7344..8081
                     /locus_tag="IW728_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025234.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8071..8793
                     /locus_tag="IW728_001882"
     CDS             8071..8793
                     /locus_tag="IW728_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1025235.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2956 a         1514 c         1861 g         2536 t
ORIGIN      
        1 ttaaataaag tagtgaaaaa taagaatcag tgctctaact gttcaagagg atcaagtggt
       61 tcaaatgttt ctgtagtctg ttttgcttcc aattggggca aaacagatcc aatttccaga
      121 tacttcactt tattcaatgt ataagataga atagaacagc taagtaatgt atacagaaaa
      181 tgtgtgagat ccaaataaag caaggagaca actaaaatac caaaagtaat caagaaaaga
      241 cttttgccaa tcttaatatg aaaatatttt tcaatggctt gataaatgat atctgttccg
      301 cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag aatacctgtc
      361 atcaaactat tgatcagtag actatctaca gcaaaatgta tatgaaggct ctcccataaa
      421 tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc gtgttttcca
      481 aaaagaaaca atgagataag caataaaggt aaattaaaaa gcagtgaagt gacagaagga
      541 gcgaatccaa aaagattatg caaaagcaaa gcaataccag taaccccacc atctgccagc
      601 ttagatggaa tattgattcc tgtcacagct aatccgtaga ttcctgcgcc taaaatgatt
      661 ccgcacaatt ctgtaaataa atttaatttt ttcatttcta gcctcttttc ttttgaaaat
      721 tttcagcaac ttctttatta tactgactta tactcttact tcaagaggaa aatgaaaaaa
      781 aattattttt ggctgtttct ttttatttat caaataagga gtggaaaaat gcgagcagca
      841 gacttattga caattcttac aaaatcaccc aaaaaaacag agtatcaagg agtttttttg
      901 gctcaagaac aaaaactggt gcctgtgaca atggtccaga taaataaagc aaatcaattt
      961 atctttttat ttgaacacgg caaagcacca cttgctatga aagacatctt tctttttttg
     1021 atgaagaacc gtgagaaagc tgtttattat cagtcaaaag aagaactcat tcctctttat
     1081 ggagtgaaag agatagaaaa taaagtggtg atataagtaa aaataagaaa acaagacgtt
     1141 tatgatgtca aaacaaccat tttttacatt tttggcaaaa aagtgtcatg atacagtata
     1201 atattcgttg aacaggccaa taaaggtgtt ctcccccacg agatttctac tttattggct
     1261 gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag atatagctga ttccaagtga
     1321 caatttcgta tagattttta tggtattcgt agtgaaaaaa cgagaaccaa aaagttgatc
     1381 cgactttgtt gcttggattc tttttttgtc agcatattta gaggtatttt tttctttttt
     1441 ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg gaaaaaggaa agaaatgttt
     1501 atacttaaag aataacagaa aggaaggtcg catgaataat tttatacaaa cattaagtga
     1561 aagaaaaggc gagttgctgg aagctgcatt tcaacatctg tccatttctc tcatttcttt
     1621 gctgattgca gcactgattg ctattccttt agcgatttgg gcagccaatc ataaaaaaat
     1681 ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg attccttcac ttgccttatt
     1741 aggtttactt atcccatttg tagggatcgg aactgttcca gcattgattg cacttgttat
     1801 ttacgctttg cttccgattt tccaaaacac gtatattggt ctagctgaaa ttgacccgtc
     1861 tattgaagaa gcagcggtag catttggtat gtccagaatg agacgcttgc tgaaagtaga
     1921 gcttcccatc gcattacctg tcattatctc aggtatccga actgccttag ttttaatcat
     1981 cggtacggct actttggcag cattgatcgg agcaggtgga ttagggacat ttattttgtt
     2041 aggtattgac cgaaatacac ctgttttgac attgattggt gcgatcagtt ctgccttgct
     2101 tgcaattata ttcagcggac tgattcgctg gttgcagcat aagaaacctc gatattccat
     2161 cattacttta gcagcagtgg tgtgtttatt tggcggcgtt tctttatatc agtctgcctt
     2221 gtttaaaaat gaaaccatca cgattgctgg aaaattagga gcggaacctg aaatcctgat
     2281 ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca gaagttgaat taaaaccgaa
     2341 ttttggcaag acgagttttt tgttcaatgc actggatcaa ggtcaaatcg atatctatcc
     2401 tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta cctgaagcaa tgaaaagtgc
     2461 tgatttgaca aaagaaaaaa catatgacga agcagaagaa ttattgaata agcaattcga
     2521 tatgacatta ttaaaaccaa tggcttacca aaatacgtat gccttggcag tcaagagatc
     2581 ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg gcagctatct ctgatcagat
     2641 caaagcggga ttcacactgg aatttattga tcgttcagat ggatataaag gaatccaaga
     2701 agcatatggc ttgaattttt caagtgtaca aagcatggag ccaagtttgc gttaccaagc
     2761 aatcaataat ggagatgtca atattgtgga tgcctattcc acagatagcc agctcaagga
     2821 gtacgatcta gtgactctag aagatgatcg agggttattc ccagcttatc aaggcgcacc
     2881 gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc gttaaagcac tgaataaatt
     2941 aagcggaaaa ataacagagg atcaaatgat tgatatgaat taccaagtca atgtcgaaaa
     3001 gaaaaagccg acagaagtag cacgaatctt cctgaaaaat gaaggattag taaaggagga
     3061 agatcagtga ctaaattaat cgagttcaaa catgtgcaaa aaaagtatga tggaaagtat
     3121 gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa tctttgtttt ggttggtcct
     3181 tccggtagtg gaaaaacaac tactttgaaa atgatcaatg gattatctaa accatcagca
     3241 ggagacatct acttcaaagg aaaatcgtta aatgaatata atctgcagaa aatgcggtgg
     3301 aatatgggat atgtgctgca gcaaatcgct ttgttcccga ccatgacagt caagcaaaat
     3361 attgaagtca tccctgaaat gttaggttgg gaaaagcaga aaagagcaga ccgtgtagat
     3421 gaacttcttc aaaaagttgg cttatctcct gatatttatc gagatcgtat gcctagagag
     3481 ttgtctggag gagaacagca acggatcggg atcatccgag ctattgctgc aagcccagat
     3541 gtgattttga tggacgagcc atttagcgca ttagatccga tttcaagaaa ctcattacaa
     3601 gaattggttt tgtccttaca cgaagaattg ggcacgacca tcgttttcgt tacgcacaat
     3661 atggaagaag caatcaagct aggtgatcgt attgcattta tgaaggatgg agaaattatc
     3721 caatgtgaca caccagagca gttattgatg aatcctaaaa acgactacgt tcggcatttc
     3781 tttgatgaac cgaaacagac gaaagagtgg cgagtagaag atttagtcgt caatggatat
     3841 ttcttaaatg aaataccaga aaatgctcgt caacaagttt gctttgatac gcctatgaaa
     3901 gaagtatatt cgcttctttc ggtgtatcca agcattgtga tagtagaaaa gcaaagaagt
     3961 attgggtcgc tgacaagtaa agaaatcttt gcttttttaa gtcgagagga ggaaggaaat
     4021 gaaaacatat gatgcaatca tcatcggttc aggtgtgagc ggcttaagtg ctgcttatgg
     4081 attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa gatctatggg gcgggacttg
     4141 tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt gcagtagagg cacgtaatcg
     4201 ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca actgccaact gggaagaact
     4261 tcaaaaattc aaacgaacat ttacggatcc agtcccagaa agccgaaaaa aacaattagc
     4321 agaagctgaa atcgatcatc tttcaggaac cgctcgtttt ttggatgatt catctattga
     4381 agtcaacgaa gaagtcttcc atgcggatta cttagttttg gcaaccgggc aaagacctac
     4441 gatcttacca gtggagggaa aggaatactt gaaaactagt gcagactttt tatcattgcc
     4501 tgtactgcct aaagaaatca tttttatcgg tggtggttat attgcttttg agctagctac
     4561 gattgccaat gctgcaggga gcaaagtaac cattgtccat cataatcaac ggccattaaa
     4621 agaatttgag gcgtctttgg tagaggaagc cgtccatcaa atggaagcat ctggcattca
     4681 gtttgctttt ggtgttgaaa cccaaaagat catttcagaa ggtactcgct atcgtttagt
     4741 cggaaaagag acggaattag tagcagatat gattttttgt gcgactggga gacagccgaa
     4801 tacagaatct ttagcacttg agcaagcgaa tatcgtcttt gataaacacg gaattgcagt
     4861 caatgattat ctgcaaacga gcaatccaaa aatttttgcg tgtggagata ttgtttcaag
     4921 gaaaacccca aaattgacac cagtcgccac ttttgaagga aattatgtag ctaagcggat
     4981 aacggatgca acaagcgaac ctatcaaata tccgattata cctacgatcg tctacgccag
     5041 tcctaaactt gctgaagtag gagttacaaa aagtcacgcc tcttcgtcag atcaagtggt
     5101 agagatggat cttaccagtt ggttcaccta tcatcgtgtc aatgaaccag ttgctaaggc
     5161 tgaattgacc tttgaccagc aaaattattt gattggtgct gcagtgatta gcgagcaagc
     5221 agacgaattg atcgatgatt tgacactcgt catcaatcaa aaattgacta aaaaagagtt
     5281 ggatagctat atcatggggt atccgacatt ggcaagtgat ctctcctatt tattgaaata
     5341 atcagcatga taactaaagc gcttgttgct ttttccttct tgtgctacac tttaaaaaat
     5401 gaatacctgt ttctggggat gtcgtagacg acatgagagt aagttgtgaa cttagaccca
     5461 ttgaacctgt cagttaatac tggtgaaggg agaaacatag cagacgcttg aagcgggtta
     5521 ctatgtcgtg cccatttcaa gcgttttttg ttattcatat tagaagaaag gaggtaacaa
     5581 aatgaagaaa aatctttatc cagcagtcag tatggcattc attttaatta tttggcagat
     5641 tgttgtcata gtttggaaaa tacctgcctt tattttaccc ggacctgctg ctgtattttc
     5701 tgcattagcg gcagatgcac aagtattact ttcccattct gtcacgacat taaatgaagc
     5761 agtgcttggt ttggtaatag ctagcgttct ttcttttatt actgccttgg caatggatta
     5821 ttggaagttt ttagaaatgt ctttttatcc gttactagtg atttcacaaa tgttgccaat
     5881 catggtgtta gggccattac ttaccctatg gtttggtttc ggtatgcttc caaaagtgat
     5941 ccttgttgtc ttgataagct attttccgat cgtagtttct ttttcggata cattgaggaa
     6001 agtatcaaaa gagcaaatca tctttttgaa aacgatgggg gcagatacag ggaagattta
     6061 tcgaatatat aaaataccag cgggtttatc cggcttcttt tccggtttaa aagtggctgc
     6121 tacttattgt gtcagcggag cagtcgttgg agaatggcta agcgctcaag caggcttagg
     6181 gtattacatg atacgcgtaa aaaacagcta ccagatcgat aaagtttttg ctgcgatcat
     6241 ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt tcattattga aaaaagggta
     6301 ttatcgtatg ctttatattt tttaagagaa ggggatcaaa tgaaaaaatt atggctttta
     6361 ttaccgctac tgttgctttt atccgcttgt ggcactgcaa aagaaacaag tagcaaacaa
     6421 gaaataaagg atttgaaaaa agtgacactt gttttggatt atgtaccgaa tactaatcat
     6481 actggcatct acctagcaaa agaaaaaggc tattataaag aggctggttt aaatgtccaa
     6541 atcatcgaac caggagataa ttcgacaagt attggtctag taggtgcgga caaagcacaa
     6601 tttggtgtaa gttatcaaga agatgtcact tatgctcacg cagatggaca gaatatacca
     6661 gtcaaagcaa ttgctacagt gatcaaacat aatacttctg gattcgccac cctatcagac
     6721 agtaacatcc attcaccaaa agattttgaa ggaaaaacat atgcaggttg gcaaagtcca
     6781 agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg gcggggactt ttcaaaactg
     6841 acaatggtag gaagcaatgg agaagggcca gaaagcttag gaaaatcctc agatatccaa
     6901 tggtactttg aaggttggga tatgatcaaa gcaaaagaag cgggtattga actaaactat
     6961 atcccattga aagaattaga tgaacggtta gattactata ctccggtaat tattacgaat
     7021 gatcaactga tcaaaagtga tccagaattg gtacagtctt ttatggatgc cacgaaaaaa
     7081 ggctatcagg aagctattaa ggatccaaat gacagtgcta aactgttgca aaaatatgca
     7141 aaagaaaatg atcggacgtt tttagaagaa tcacaagcat tcttgtcaaa aaattatacg
     7201 gatgatcctg agaattgggg attgatggaa gaaaaagttt ggcgcaatta tacagctttt
     7261 atgcaggaaa acggattgat caagcaagat gtaccttcgc aagaactatt cactaatcag
     7321 tttataaaat aggagtgata gatatgtcaa tccaaatcag ccacttgaat gtagtcattg
     7381 aaaacctgaa aatattagaa gacatccatt tcactatccc taaagatacc tttacatcga
     7441 ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa aactttaaca ggagtccagc
     7501 ctatgacttc tggaacgatc aaagtagacg atcaaaaagt aactggtctc catacagcat
     7561 ttagttatat gccgcaagaa gatatgttac ttccttggct gagcgtctat caaaacgtta
     7621 cgttatatca aaaaatcaat catctaacaa tcaatgaaga acaagtaagg gattatctgg
     7681 atatctttgg gttaaacggc tatgaacatt ttttgccgga acaattatct ggcgggatga
     7741 agcagcgaac ggccttgtta cgaacaatca tgaatccttc ttcttacttg cttcttgatg
     7801 aaccatttgg agcgttagat gctatgacca gagggcagat gcaagactgg atgttgaagc
     7861 tgcctaaaaa agcaaagcgg actactttac ttgtgacgca tgatatcgaa gaagcaattt
     7921 atttatcaga caggattctt gtgctttccg cccgtccggc acatgtgatt gctgagattc
     7981 aagtgcctga aaaacgacgg tcacgtgaat ggctactgca gcaatcagag ttgaagcaaa
     8041 cgatttatca gttattggca ggtgaattag atgttaagtg atgctcattg tcatttagat
     8101 gggagtcaga gtttagcact ccttcagcag gaacacacga ttttgacgat catcaattgt
     8161 gattcgccag aagaatggaa ggaaaacaga cagcttgcag caagtaagac tcaagcctta
     8221 agttacggta tccatccttg gaaagcagat agctatacgt ttgaacaagt tgagcctttt
     8281 ttgaaaaaag caaggatcat cggtgaaata gggttagata atatttggac gaatgtaccg
     8341 atgactacac aaaaaaaagt atttgagcga cagcttgctt ttgcagccat aaatgagaaa
     8401 cctgttgtac tgcacacaaa aggatgcgaa aaagagatct taaattatat ccaagcttac
     8461 cctaatcact atttgatcca ttggtattct tctttggact atcaacaaga ctatattgat
     8521 ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga atccagcagt ctggcaacta
     8581 gctaaagcag tcccaatcga ccacttactt attgaaacgg atggactaca tgcagcagag
     8641 tgggctttag gggggatttt ttcagaaaaa gattatgtct cactgcttga agaacagtta
     8701 aagacaatcg ctgagatcaa agagctatca gttgaagaag ttaaaaaaaa tacttatcaa
     8761 aacttggaac gtttcattag attaggtaaa taaaaaagag atgtgacaca actagtgtcg
     8821 cacctctttt tggctttctt tgtataaaag taaactgttt gattaat
//
LOCUS       DACEMO010000110         8761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEMO010000110 DACEMO010000000
VERSION     DACEMO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.113"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..543)
                     /locus_tag="IW728_001883"
     CDS             complement(<1..543)
                     /locus_tag="IW728_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025236.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDH"
     gene            complement(549..1220)
                     /locus_tag="IW728_001884"
     CDS             complement(549..1220)
                     /locus_tag="IW728_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1025237.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(1213..1554)
                     /locus_tag="IW728_001885"
     CDS             complement(1213..1554)
                     /locus_tag="IW728_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025238.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(1732..2430)
                     /locus_tag="IW728_001886"
                     /pseudo
     CDS             complement(1732..2430)
                     /locus_tag="IW728_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
     gene            2517..2987
                     /locus_tag="IW728_001887"
     CDS             2517..2987
                     /locus_tag="IW728_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025239.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(3030..3209)
                     /locus_tag="IW728_001888"
     CDS             complement(3030..3209)
                     /locus_tag="IW728_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025240.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3196..3534)
                     /locus_tag="IW728_001889"
     CDS             complement(3196..3534)
                     /locus_tag="IW728_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1025241.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(3550..3753)
                     /locus_tag="IW728_001890"
     CDS             complement(3550..3753)
                     /locus_tag="IW728_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1025242.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(3768..4544)
                     /locus_tag="IW728_001891"
     CDS             complement(3768..4544)
                     /locus_tag="IW728_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1025243.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            4843..5163
                     /locus_tag="IW728_001892"
     CDS             4843..5163
                     /locus_tag="IW728_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025244.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            5181..5612
                     /locus_tag="IW728_001893"
     CDS             5181..5612
                     /locus_tag="IW728_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1025245.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            5742..6203
                     /locus_tag="IW728_001894"
     CDS             5742..6203
                     /locus_tag="IW728_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1025246.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            6237..6872
                     /locus_tag="IW728_001895"
     CDS             6237..6872
                     /locus_tag="IW728_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1025247.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            6985..7620
                     /locus_tag="IW728_001896"
     CDS             6985..7620
                     /locus_tag="IW728_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025248.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            7919..>8761
                     /locus_tag="IW728_001897"
     CDS             7919..>8761
                     /locus_tag="IW728_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1025249.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNF"
BASE COUNT         2908 a         1516 c         1395 g         2942 t
ORIGIN      
        1 atgatcaact tctttgcgct ttcgtcttcc tagcgctttg tcaaagtgat cgatgtcagc
       61 tcctgttttg ccacagatgc agcagactct ttttgtgatg catttgtaga agtaatattc
      121 ttgattcgca ggtaaaatct catagccttc tttgaaagga atatgatgtt caaagatgaa
      181 atctaagatg atatttgcta agacattggc atcactcaca gttgtattcg attcgtcttt
      241 caggcttatt ttgcgccctg tgacaccttc aaaacgaaag tagaagaatt ccttccagaa
      301 gtccgttggc atgcctgtat cgataaaaat atcgcctatc agcgcataga tgaagtttcg
      361 ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt tcaatgactc gatcaccatc
      421 gtagccgtca tacatcgtct ttagtcgatc aatgttcact tcctcattga tggttgcgct
      481 tatgtctttt cctttgaact ttttcagaac cgctgaatat gaatcgatta atggtttaaa
      541 cactcatatc acttcttatc taattctttt ctcttagctg ctattgctcg ctccatcaag
      601 gcacattgct catagcttaa ctgttcaata gtttcaacgt tatcagctaa gagccctaat
      661 ttatctgtct gctcattaac atattcgatt aaggttttgg tcatatcttt acccatctgc
      721 tcattgaaag cttctagaat cgtctctagc atgtttaatt tctttgtatc gattctaggt
      781 ggtgttggaa tatcttcccc ttgaaataca tataatccca gtccgtgtag agccaatgct
      841 ttcacaaagc atcgcttcaa tgagttattg atttgcattg catttggttt aacaactggt
      901 tggtttcgat aatctaaaac aggaaataat tcggtttccg tgtgtccttt aaccgttact
      961 gagacagata cataagtccc agtttcatcc ataagaaaag gtttatattc ctcaacaaga
     1021 aagtcttgat gagttccaga aacaaccctg tagtgtttgt actcattaat agttaccgtt
     1081 gcctgtggat cattcttttt cataatctcc cacgcgtgag cccaagataa ataatcaaaa
     1141 tttccttttt tcttgagaat tttatttaac ttacgactaa aaagtttttc aaagttcgtt
     1201 gtccctttga tttcactcat caaattctgc ctccatttca gcaatgtatt tcttacctgg
     1261 tccgtaataa gagatatcaa tcaagttatc tctgtcgtac tcttctagcg catcaatcaa
     1321 gccatcttcg atgacgtaaa tatattcagg tttattcgaa tgcttcgata gatggataag
     1381 ataaacatga tcccaaatag ttacatggtt gcctaaatca tcttgatccc aagctagttc
     1441 ttcattcgtc aaaagatttc gtctgatttt tcgaccactt gtttcctcaa ttttcggctt
     1501 gccccaatca ggatcaatca aatattgatc tagagtggaa agttcttttt ccatatgcta
     1561 aaatctcctt atgatgtgtt ttctttgtga ctctttgctt gccggcggag tcactttttt
     1621 atttgttgcc atgctttttg cttgtcaata tgttgttggc ttaggatgat tggtttatag
     1681 tatttccacc agcaattagc aattgccgtc cctattctta gcgcttcagc tctattcatt
     1741 gtcatcacca aaaagtctct gttgtctgtt cagttgatca atttccatac ggatcgcagt
     1801 ttctggcaac cacatttcaa taaatgaaac agcatcatcg aatctcttac gaggtaactc
     1861 gccatatctt gggattgaaa aggtacgttt aaattcagac caaaattttg agaatacttt
     1921 tttgctgatt tcttcataag ctcagctttc ttttccccct agaactccca taactttcat
     1981 atttcctttt tgcttaattt caaactcttg ttgtccgcta attcgcatag tatctttaag
     2041 catggaaaca tcttttttaa catctttcat ttcttctaat tgatagatca tcatatcttc
     2101 aattgtttga ggaacagtat tcttgcgaat aacatcttcc atttcgttga atgcttcaat
     2161 gtatttttgt ttgaagtaga tagctttctt tcctgtaaac cccatagcca gcaagaaaaa
     2221 tccatctcta ctaatgaaga aaactcgtcg atttctgcca tatgaatctg gttcattacc
     2281 ttctacaaac atctgtccaa aattggacac atcttttttt agtgcatcaa tatctcttaa
     2341 aacatgttga tgttttttct cgaagctttc tgccacttgt aagctcgtag tcacagcttc
     2401 tttatttttc aaaattacta attcttgcat tatttcttct ctcctttttg gtataattta
     2461 gttaaaaact ggtggtgttt attttgaatt tttttcactt aacaattttt caatggatgt
     2521 caataataag ttttatattg agtacgtttt taacagggat aaaaataaaa ggaatgcgcc
     2581 ctcaactaga agttgaactt aatgcttctt acttcgctgc agatatgatt tatacaaaag
     2641 taatcatttc taactattca acagaaccag ctatgttagt taatttagaa ctttcatccg
     2701 ctaacttaaa ccgcagatgg tcagctactc catttaagaa attaattgct aaaggaggat
     2761 cagtcgatga taatagaata tactctgaag ctgtaccgct aaatattcct cctaaaagtg
     2821 ctatatcttg ttaccttgca tttgaagtag ggaaaattaa ttttaataaa ctccttaatc
     2881 accaaacaaa aatgatattt actctaaata gaacccaaat ccacaaaatt gttgatatca
     2941 aaaatacaaa cttcccaata gagaaattag tgaaagaatt aaactgagta atagtaatat
     3001 acattctagt ttctcttttc tattcatttt tagtcagccc cctcggttgg ctttttcgct
     3061 ctgtactcag cttcagcttc atcaagcccc ataaaaatcc aaaccatgta aacaatcgtt
     3121 cctatcaacg cttgcctgct tccccaaagt cctaaagcgt agatgattag cggtgcgctg
     3181 aacactagcg ctctattaaa ttttcccatc cgcttgcctc cttaagattt ccgaaaaatt
     3241 tgtttctaaa aattcaagtg ttttacttct taaaaataga tatgtgtctc ttccttcaac
     3301 tggataatag acaaatccat ttttgttttt ctcgatatcg ataatatttc tatatcttgg
     3361 gttctttaaa actctagaag taaaccaatc atattttctg ttaatccgtt ctagcacttc
     3421 tggcaacgtc atccatctac cagtatcatc agcttttttt aactcctcat aatccacttg
     3481 ggagataatt acatagcctt ctggaattgg gatttttgct tctagatatt gcattagctg
     3541 ttcctccctt tacaattcgt acatagtgat aatcgaatct ataattctgt ttgcttctgc
     3601 agaagtcttt ttaccgttta aaattaaaga taagtagctt ttgctaattc caaatctttc
     3661 agcaagcatg gtgtaagtta agaactttga actttcgaca tattctttga ttttttctct
     3721 atctcgttga gtgatttctg caatgtcagt catactaaaa ctcctttcta accaatttcc
     3781 tctaaatcca tttgagggta atatccttct ttttttagta attcgtagat aaatagacgc
     3841 cctttctgtg tccatttggt attcattaca attttagtgc caccatcggc tttcgggatc
     3901 tcagttgtat gagattttgt gtatccttgg ttcatgtgtt ttttgcacaa taaccattgg
     3961 ttaccgactt ttttctgaat acctagttta tgaagtaatt tattcatctg ttgtggagac
     4021 atcccataat ctgctgcaat ctgactaatt gttactgaat ctgtagaaga taatatgcta
     4081 tctaaatagg agattttagg ttcgtactct gcaatctttt gttctgcgat taatcttcca
     4141 gttctttctt cttttagttg agttgctaat tgaatgattg tatctggatt aagcaaagct
     4201 tcttctactt tttctggagt tagataacct ccatgttttc taattgctgg caacacttca
     4261 cttgttaccc aacgtttaaa ttttttggca gagggaagtt ttgattttaa gattaaactg
     4321 tacaaaccag attcgttgat gattgtcatc tctcttgatt gacctgaggt cgtgattcgc
     4381 gaccccatct tatcttccaa atctacatgc cgcgacaaag catctttagt gtttgaataa
     4441 cccaaaacac tcgcaacgtc tttgcctaca aaatatggtt catcatttac taaaattgtc
     4501 cgaacttcgt tttgttcgaa attaaaaatt tgcggtgtgt tcatatttct cattcctttc
     4561 ttttatattc gtaaacaaat ttaacaactt ttttctaaat tcaattgaca gtatctgagt
     4621 aatactctat actaagagca taattaaata agacataaaa acattgattt taaagctttc
     4681 ttggcggtct gcatttacat atcaatagtg ttttttgttg tctttttagt tgttaaatct
     4741 gtttacaaga tatagtatag ctcattactc agaattagtc aaattatttc tgcgtatttt
     4801 tctaaacttt ttttgtaaac atttagaaag gttgttttat cgatgaatac ctatgaaata
     4861 ataaaagact tggcgaagag gaaaaagata tctatacggc agttagaaat taattttgga
     4921 tattctaacg gttatctagc aaagtggaaa aataatactc ctaattcaga tgaattaagt
     4981 cggattgctg actatttcga tgtctctgta gactaccttc taggtagaga agaaagagaa
     5041 acccctaaac atgtggattt atcagaagac gatactgttt tttcttttga tggaaaagaa
     5101 atatctaagg agacaatgcg taaagcgatt gcaattgcta aagctttaga ggaaaatgaa
     5161 tagttggagt gatggtttgt atgtatttga aattgaaaga aatgttgagc gaatacaatt
     5221 tgaaagtagt ttatatggag atgaaagagc ctggtttcta ctatccaaaa cccagaatca
     5281 tttttttgaa tgaaaatcta tacggtgaaa ctgcagaagc ctttcattta tctcatgaac
     5341 ttgcacattt cagcgcctct cattttgaat tttcagtttt gtacgatact tctacaactt
     5401 ttcactcaaa atttgaaaca gaagcagata aaatagctat tctaatttta ttaaatatct
     5461 atattgagaa cgaactgact gatgaatctc agtttaatct cgaaaaattt atggagtatt
     5521 attctattca gaataagctc agatacactt gttatgctgt ctgccaatgc tattttaaga
     5581 aaaaatattc ttacgcaagg caatatgtat gaatacatca agaattatta aaaaaaccga
     5641 tttcagcaaa acattaaaaa agcccgtgct gcaacacggg ctctttcctc atatatgagc
     5701 ttctacaaaa aaaatcatat cacagaaatg aggaagaaaa aatggaaaaa gagattttaa
     5761 taaaatctcc aggaaaaacc ttgattaaag taactgatga ttctatttca attattagaa
     5821 aaggatttat aaatttagtt aatcaaggta ttaaaggaga aaaaactatc ccctttaaaa
     5881 atatatcagc cgtacaatta aaaaaaccag gtatgagtaa tggttatata caatttacct
     5941 tgctaggcgg aaacgaaagt cgcggaggta tattagcggc cacgaaagat gaaaacaccg
     6001 ttatgtttac aaaaaaatat tggaatgaaa tggaaaattt aaaaaaatat atagagaagc
     6061 aacaatctat attagataat aattccaaag aaacacaaat ttcaagtgca gatgaaatta
     6121 aaaagtataa agagttgtta gatgaaggga ttattaatca agaagaattc gattttaaga
     6181 aaaaagaatt gcttggttta taatattcaa tatcaaaaga gaaagaggat ctattaatgg
     6241 acggtatatt tggttttact ggtattgttt tatttattat aggtttaatc atgttaattg
     6301 taagatttat caaaaaaaca aataagaaaa cacctatgat tctccttatt atcgggatta
     6361 tttttactac cgtaggtttc tcgctttcct catcaaacga ggcaaatgac aataacaaac
     6421 aggaaagctc atcatctgat actcaaacta ctacatctaa cagcagcaat aaaagcctaa
     6481 ctcaatttga agaattttta gagacaaata atcatgactg gggtgctttc ttagatagtt
     6541 attattctat tactccagct actgagcaaa atacagcttt tactaaatat attagtggta
     6601 aaacatatac ttttgaagga actgttatcg aatcaatgac tactagaata gccattatag
     6661 cagacaaaga gtacgatgat aaaagttgga atgatatttc tgcaacacct aaagtttcat
     6721 atgtaatttt tgcgaaggac ttaaacaatg ctgatacctt tacaaagggc gataaagtaa
     6781 cttttacagg agaaattagt tcaagaggat cgaacataga aaaatcctat gctcaatggg
     6841 atatgattaa tagtaaagta agtaaaaaat aaatgaacca cgccccacta agagttagat
     6901 tgtgcacaat aataaaacct atacaaaaga gtgccatcag gagtcacaat gcttgtaaag
     6961 gaaatgacaa aataggaggt aactatggat aatagcaatg aaaaactaac caaaaaagaa
     7021 tatcttgaat taatagttga aggcggtatt ggagcaattc cttatattgg tggcgttgtg
     7081 cagtcacttt attttgggtc taaaaacgag aagcgtttca aacgcatcga gaaattctac
     7141 aaaaatttaa acgaatcatt agaaaacatt cgcgatcaaa ttccagaaga tgttttcaac
     7201 ttagagaaca aggaccaact cattggaata ttcgaaggga taaatgacga ggtagaaaaa
     7261 tcaaaggcac aaaataaaat agatatgtat aaaaatcttt ataagaattg tttacttcga
     7321 attaacagcg cttcatggga taacgaggag tattatcttc ttgtactgaa tcaattaaca
     7381 tcgattgaga ttcaattgtt agcatgctta atgagtcgtg gaaacgaaaa tttcactgga
     7441 aatatcagca tgtctggcta ttcacaagag ctaatagatg gtagcttaaa tagattatct
     7501 gattttggtc tgttagaaaa aacgattagc tccatagtat taggtgatgt cggcaaacaa
     7561 aatatgggct acaaaatatc tcatttagga attcaattta ttaactcaac tcttacttaa
     7621 gttttctttc taatatgaca tctagaccta tcagcaatgc aacaataaaa aaccagacat
     7681 accaataatc aacaaataat ctaaactctg gaagttcagt tattctttta aaaatcactt
     7741 taatcataat aaacacctct gattggatga tctaatacac atccaattat accattaaaa
     7801 cgacaaatag cacatccttg cgccggcaag caaaaaggat gtgcaaaaaa taataaaacc
     7861 caaacaatgg gcttctttat agttcctatt gtatcagaga aagagagctg attcaattat
     7921 ggcaagatta gtcaaacgtg gaaatagttg gcaatacgaa atttcataca aaaaagatga
     7981 cggaaaatac acgaagataa gaaaatcagg atttaagaca aaaggagaag caaaagatgc
     8041 cgccaacgaa ttagaatata acttgaacaa aggccttaaa ggggatcgca aaaatctatt
     8101 attatcagat tactttgagg attggatgca actttataaa gaaggaacag tatctcctat
     8161 cacttataga aaatacgaag atacgttaat gaacataaag aaatatatgc cagcggtatt
     8221 gatttctgat ttagatagag tgggatatca acgcttttta aataagtatg ctaaagacca
     8281 tgtaaaatct accgttatta agtttaataa ccatattaga gcgtcgttga aagatgccgt
     8341 agaagaagga ttaattccgt ttgatccaac tagaaaagca gtaatcaaag gaaaagattc
     8401 attgaagcca aaagaagata aatatttaga ttatgatcaa tttaaatctt taatgaaact
     8461 cgtagaagaa aacctttctg cgcagtactc ttctcctatg ctcgtgttag ttgctggtgc
     8521 cactggaatg cgatttgctg aacttctagg attaacatgg gaagatatcg atttcgaaga
     8581 tcaaatcatc actattaata aaacatggaa ttataaatta aatgaatggg gaaaaacaaa
     8641 aaacgaaact tcaaatagga aaatttccat tgataaacat acgattgatc tcttaaaaaa
     8701 gtttaaaatc aatcaaaaag aattattcga gaattttgaa atcaaaaacc ctcataattt
     8761 t
//
LOCUS       DACEMO010000111         8731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEMO010000111 DACEMO010000000
VERSION     DACEMO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.114"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(74..1030)
                     /locus_tag="IW728_001898"
     CDS             complement(74..1030)
                     /locus_tag="IW728_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1025250.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1134..1736
                     /locus_tag="IW728_001899"
     CDS             1134..1736
                     /locus_tag="IW728_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1025251.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1824..3323)
                     /locus_tag="IW728_001900"
     CDS             complement(1824..3323)
                     /locus_tag="IW728_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1025252.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3339..3863)
                     /locus_tag="IW728_001901"
     CDS             complement(3339..3863)
                     /locus_tag="IW728_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025253.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4161..4910
                     /locus_tag="IW728_001902"
     CDS             4161..4910
                     /locus_tag="IW728_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1025254.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4901..5686
                     /locus_tag="IW728_001903"
     CDS             4901..5686
                     /locus_tag="IW728_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1025255.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5655..6371
                     /locus_tag="IW728_001904"
     CDS             5655..6371
                     /locus_tag="IW728_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1025256.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6473..7492)
                     /locus_tag="IW728_001905"
     CDS             complement(6473..7492)
                     /locus_tag="IW728_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1025257.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7511..8569)
                     /locus_tag="IW728_001906"
     CDS             complement(7511..8569)
                     /locus_tag="IW728_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1025258.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         2628 a         1766 c         1554 g         2783 t
ORIGIN      
        1 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
       61 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
      121 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
      181 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
      241 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
      301 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
      361 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
      421 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
      481 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
      541 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
      601 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
      661 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
      721 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
      781 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
      841 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
      901 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
      961 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
     1021 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
     1081 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
     1141 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
     1201 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
     1261 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
     1321 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
     1381 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
     1441 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
     1501 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
     1561 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
     1621 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
     1681 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
     1741 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
     1801 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
     1861 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
     1921 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
     1981 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
     2041 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
     2101 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
     2161 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
     2221 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
     2281 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
     2341 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
     2401 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
     2461 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
     2521 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
     2581 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
     2641 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
     2701 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
     2761 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
     2821 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
     2881 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
     2941 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
     3001 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
     3061 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
     3121 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
     3181 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
     3241 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
     3301 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
     3361 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
     3421 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
     3481 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
     3541 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
     3601 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
     3661 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
     3721 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
     3781 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
     3841 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
     3901 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
     3961 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
     4021 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
     4081 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
     4141 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
     4201 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
     4261 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
     4321 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
     4381 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
     4441 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
     4501 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
     4561 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
     4621 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
     4681 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
     4741 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
     4801 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
     4861 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
     4921 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
     4981 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
     5041 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
     5101 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
     5161 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
     5221 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
     5281 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
     5341 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
     5401 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
     5461 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
     5521 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
     5581 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
     5641 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
     5701 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
     5761 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
     5821 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
     5881 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
     5941 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
     6001 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
     6061 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
     6121 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
     6181 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
     6241 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
     6301 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
     6361 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
     6421 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
     6481 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
     6541 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
     6601 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
     6661 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
     6721 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
     6781 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
     6841 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
     6901 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
     6961 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
     7021 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
     7081 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
     7141 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
     7201 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
     7261 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
     7321 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
     7381 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
     7441 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
     7501 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
     7561 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
     7621 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
     7681 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
     7741 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
     7801 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
     7861 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
     7921 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
     7981 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
     8041 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
     8101 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
     8161 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
     8221 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
     8281 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
     8341 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
     8401 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
     8461 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
     8521 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
     8581 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
     8641 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
     8701 aacttatgta aagtgatttt tactagaaca c
//
LOCUS       DACEMO010000112         8688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEMO010000112 DACEMO010000000
VERSION     DACEMO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.115"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            79..1125
                     /locus_tag="IW728_001907"
     CDS             79..1125
                     /locus_tag="IW728_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1025259.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IW728_001908"
     CDS             1474..2106
                     /locus_tag="IW728_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1025260.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IW728_001909"
     CDS             2694..4133
                     /locus_tag="IW728_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1025261.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IW728_001910"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IW728_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IW728_001911"
     CDS             4895..5110
                     /locus_tag="IW728_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025262.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IW728_001912"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IW728_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IW728_001913"
     CDS             complement(5770..6681)
                     /locus_tag="IW728_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1025263.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IW728_001914"
     CDS             complement(6700..7704)
                     /locus_tag="IW728_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1025264.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..>8688)
                     /locus_tag="IW728_001915"
     CDS             complement(7701..>8688)
                     /locus_tag="IW728_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025265.1"
                     /translation="TTAFSISTMFFNISATYPFFMVAFLQIVTFAGIYFKLGDIMSMF
                     NLQSNDSQSMGRRVMRKPQMLMNRKLRQLNRNVGRALAFGGGAAVGNKLAKEQSKSKF
                     KPSGSSLRKNSRLPNDHEILSDLTKENPVSNNKKQSRMNLAGRKIGKVLDTQALVKDK
                     TKQVKDQVRNTPTNLKYNLHKGIEKSKKAPEEFKRGLFQEKADRVDLREKQRQRRNAK
                     MDEKRKILSEATNRQGKRRANIPIDKAAQPQKDMMLKKETPKRIVRANLNPEIKRKLT
                     SQEIISKGKSQSASKKNSFQQVKQRSTLPKQTNQKVQKRMNHRPKSRSGEKK"
BASE COUNT         2641 a         1544 c         1670 g         2833 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcga agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgtttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgta
//
LOCUS       DACEMO010000113         8545 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEMO010000113 DACEMO010000000
VERSION     DACEMO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.116"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..444)
                     /locus_tag="IW728_001916"
     CDS             complement(<1..444)
                     /locus_tag="IW728_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1025266.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVT"
     gene            701..4090
                     /locus_tag="IW728_001917"
     CDS             701..4090
                     /locus_tag="IW728_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1025267.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            4093..5514
                     /locus_tag="IW728_001918"
     CDS             4093..5514
                     /locus_tag="IW728_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1025268.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            5511..7388
                     /locus_tag="IW728_001919"
     CDS             5511..7388
                     /locus_tag="IW728_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1025269.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            7480..8322
                     /locus_tag="IW728_001920"
     CDS             7480..8322
                     /locus_tag="IW728_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1025270.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
BASE COUNT         3076 a         1620 c         1620 g         2229 t
ORIGIN      
        1 ggtaacaaag atacgcaact ggagctcact tttaaggcta gttaactgaa aaccttggtc
       61 aacgatcact tttaataaat agctttctaa aatttgattg cattgtttca acttacgtgt
      121 aaaagaagac cggctgatgg aaattttttc acaataatac tcaatcgttt tacccggatt
      181 gacaataatc tcatttaata aacgactgtt tgtagaactt tgcgacattt ccatcagaac
      241 atttaaataa gctggtacat tcaaatgttc aagacgatac cccaattttt gtgaagattg
      301 aatcgttaca tccgttcccc agcggatttt gatctcttcg atatacctta caaatgtaga
      361 ttctgaacta ccaattcgtt tggcaagttc ttttttagtc atccacttgt cagaaatgga
      421 cagaattcga atgatttttt ccatctttct atagtttcgt tccaacaacc tgatcatatg
      481 tttttctccc tcaacccttt tctttcaatg aaacgctcat taatcgaagg aatcaacttc
      541 ataaatgaaa ccaattggta tgaaaattgg tgtgattccc tttagcgccc ttcgtataat
      601 aattatcata ttaaaataaa taaaattatg taacgcctgt ttattttaat aattttcact
      661 attcacgtca aaacagaaaa aaagaaaaag gaggggaaaa atgacgacca cagggaagaa
      721 actgaaagtt attttcatgc tgataatatt gagtttatca aactttgtgc cattatctgc
      781 aatagcagac actacagatg atccaacagt tttagaaaca atttcagctg aagtcatttc
      841 ggatcagtct ggaaaaaaag cactgaacat caagctaaat gcgaataaca ccagtgctga
      901 aaagatagaa aaagaaattg gtctagtcga aaattactta agtgatgtgg aaagaaaaga
      961 aggagatggc tatgcttatc aggtaaatag cgggaaaatt acgttggaaa tctcatcaaa
     1021 cactaaacaa actatcgatc tgagttttcc aatcgatcca gcactttacc acagccaggc
     1081 aaacaagctg atcgtcgata ataaagaata tgacattatt gatgagacag aaaataagaa
     1141 agatacagat gtgtcagtac caaagccaga cgaaatagaa gaagaatcat caaaagaaaa
     1201 cgaaaattct gtcagcccat ttacattgcc tacattatcc ttgccagctg tgagtgtgcc
     1261 atctaatcaa acgattccta cagaatatac aacagatgat cagggcactt atcctaaagc
     1321 cagttggcaa cctacaggaa atacaaatgt tcttgatcat caaggcaata aaaacggaac
     1381 aaatcaatgg gatggtataa attcttggaa tggagatcct aatgatcgga cccattcgta
     1441 tatcgaatat ggaggaaccg gtaatcaagc agactatgcg atacgaaagt atgcaaagga
     1501 aacaagtaca cccggattgt ttgatgttta tttgaatgct cgtggaaatg tacaaaaaga
     1561 tatcacgcct cttgatctcg tattggtcgt agactggtca ggaagtatga acgacaataa
     1621 tcggatcggt gaagtaaaga ttggtgtcga tcgttttgtc gatactttag cagatagcgg
     1681 tatcacagac aaaatcaata tgggatatgt cggctactca agcgaaggat atagctacag
     1741 taacggtgca gtacagatgg gttcatttga ttcagtgaaa aatcaagtaa aatccattac
     1801 accttcacgg acaaatggtg gtacttttac acaaaaagca ctaagagatg caggaagcat
     1861 gctatccgtt ccaaatggac ataaaaaagt gatcgttttg ctgacggatg gtgtaccaac
     1921 attttcctat aaagtacagc gggtacacgc acaatcaagc agcaattatt acggaactca
     1981 gttttctaat acgcaagatc ggccgggaaa tacttctcta atctcaagaa tctatgatgc
     2041 acctgaccaa aacaatctat ccagaagaat cgacagtacg tttatcgcaa ccatcggaga
     2101 agcgatggca ctcaaagaac gaggaatcga aatacatggt cttggcatcc aacttcaaag
     2161 cgatccggca gctggtctct caaaagcaga agtagagtct cgtatgcgac aaatggtttc
     2221 atcagatgaa aaaggcgatc tttactatga atcagctgat catgcaacag atatctctga
     2281 atacctagcc aaaaaagctg tacagatctc agcaactgta agcaatggac aaataaatga
     2341 tccaatcgca gaaccattca tttatcagcc tggtacactt tcagtcaaga gtgtggggac
     2401 aagtcctaca acggtcactc catctatttc catagaagga aataccatca agagcaatca
     2461 gatctattta ggaaaagacc aagaaatcca aatccattac caagtgagaa tccaaacaga
     2521 aaatgaggac ttccatccaa atttctggta tcaaatgaac ggcaggacaa ctttccagcc
     2581 aaacattgat accaatgaat tagctgaatt cggtatacca tctgctaaag ctcccggagt
     2641 cagtcttcac atcaaaaagt tatgggaaga atttgacaac aatctagctg atcgtccaga
     2701 tcaagttact tttgagattc aacgggaaca tacgacaaat gctgcagctt ggaaaaacgg
     2761 atatattcga atcattaaac cagctaaaga tacaacaaat acgtgggaac gtgcagacat
     2821 tgacaaatta tctgcaaata gcggagaaag ttatcaagag atattatcac tacctcaata
     2881 caataatcaa ggtcaagcat tcagttacca aacaatcaaa gaattacctg taccaggata
     2941 cgattctcaa caaatagatg caatgacatg gaaaaatact aaacaattca caccgttaaa
     3001 cttgaaaata acgaaaaatt cctctacagg tgaaaaggat cttattggcg ctgttttcaa
     3061 attaacagga gattctattg atactttact aacagatcat ggcgacggaa cctattctct
     3121 tccagaaaat gtcaaattgc aaaaagaaat gacctatacg ctgacagaaa caaaagctcc
     3181 agaagggcat ggattaagca aaaagactac ttgggaaatc aagatcgctt ctgatggtac
     3241 ggtaaccatt gatggaaaaa cagtcactac ttccgatgat acgatccagt tgactattga
     3301 aaatcctttt gttgaagttc ctgtagcagt acgtaagtat gcgatgcaag ggacggacaa
     3361 agagataaat cttaaaggag cagcattttc cctacagaaa aaagaagcaa atggtactta
     3421 tcagccaatt gacagccaaa caacgaatga aaaaggtctt gccagttttg attcactcac
     3481 acctggtaaa tatcgagtcg ttgaaacagc tggtcctgcc ggatatgata cttcgccggg
     3541 aaattatgaa ttccaaatcg ataaatatgg aaaaatcatt tacacgggaa aaaataccga
     3601 gatgacaaat aatgtatgga cgctcactca tcaaaatcga ctaaaagcgt ttgatctaac
     3661 ggtacacaaa aaagaagaca acggacagac attaaaagga gcaaaattca gactgcaggg
     3721 accagaaatg gacttagaat cgccaaaaga tggacaagaa acagatacct ttctattcga
     3781 aaatttaaaa cctggaactt atacgctgac cgaaactttt acaccagaag gataccaagg
     3841 tctaaaagag ccagttacta tagttataca cgaagatggg tcaattcaag tggatggaca
     3901 agatcatgaa tctgttctgt caccaggagc caaaaacaac cagatttctt tagacatcac
     3961 gaatcaggca aaagtaccat tacctgaaac gggaggaatt ggccgtttag gaatctatct
     4021 agtagggatg attggttgtg cgttttctat ttggtatctt tttttgaaaa aagaaagagg
     4081 gggcagctaa atatgaaaaa acttggttgg cttagtatgt gtctcttctt gttactattt
     4141 aaaccagctt ttactcaggt agcaacagaa acagaaacag aaatggttca gattacttta
     4201 cacaaattgc ttttcccaaa cgggcaactg ccgaaaaatc atccaaatga cggacaagaa
     4261 aaagctttat tacaaacgta tcgaggatta aatggtgtca cattccaagt ttatgatgtc
     4321 acagattctt tttaccatct acgggaaaag ggcaaaacgg tagaagaagc acaagcagag
     4381 atcgcaaaaa acggtgcgtc ttccggtatg tttaccgcag aagcaacaac tacaactctt
     4441 aacaacgaag atggtatcgc ttctttttct ctggccgcta aagatcaaga aaaaagagat
     4501 aaagcgtatc ttttcattga atccaaagta ccagaagtcg tcaaagaaaa ggcagagaat
     4561 atggtagttg ttcttcctgt acatggacaa aacaatcaaa aactttcaac tatccatttg
     4621 tatcctaaaa atgaagaaaa cgactaccct gatccacctt ttgagaaggt attagaagag
     4681 cctagaaatg attttacgat tggtgaaaaa atcacttatt ccttgcatac gacaattcct
     4741 gtaaatatcc ttgactatca aaagttcgaa ttgtcagata gtgcggatga agcattaacg
     4801 tttttaccta atagtttaac gatttcatcg aatggagaaa agctgacaga aggctttgtc
     4861 atacacaaga aacctcacgg atttgatgtt ttattttcga tcccttcgtt ggaaaaatat
     4921 gctggaaaaa aactgaccat ttcttatcag atgcagctaa gcagtacagc acaggcgaac
     4981 aaggaaatca acaacaacgg aacactggat tttggttttg gtgtcagtac aaagaaagtc
     5041 tctgtatata cagggagtaa gcaatttgtc aaaatcgaga caaataaacc agataaacga
     5101 ttagctggcg cagtattcct tattaaaaac aaagcaggaa attacctcca gcaaacagcc
     5161 aacggataca agtggacaaa gaacgaatca gatgcgcttc acctgatttc cgataaaaat
     5221 ggcgcttttt caatttccgg gttgaaaaca ggaagttatc gattaaaaga gatcgaagca
     5281 ccttctggtt atattttaag tgaaacagaa attccgttta ccatttcaac ttttctttct
     5341 gaggataaag aggcggacag tatattgaaa gtagtcaata aaaaagaaaa tagccgtcca
     5401 tttcttccaa aaacaaacga aacgaaaaat acacttttag gcgttgttgg tatggtattc
     5461 gcaagctttg caatctggtt gtttatcaaa aaaagaacag gagtgaaaaa atgaaaaatc
     5521 ataaaaaaat aaacgttatg ttaggagtcc ttttccttat tttaccatta ctcacaaaca
     5581 gcttcggcgc aaaaaaagtg tttgcagagg agacagcagc tcaagtcatc cttcataaaa
     5641 agaaaatgac tgatttaccc gatcctttaa tccaaaacag cgggaaagaa atgagcgaat
     5701 tcgatcaata ccaaggatta gccgatattt cattttcagt ttataacgtc actcaagaat
     5761 tttatgcgca acgagataaa ggagcgtccg tggatgcagc aaaacaagca gtccagtctt
     5821 tgactcctgg tacaccagtt gcttcaggaa cgacagatgc tgatggaaat gtcactttat
     5881 ctttacctaa aaaacaaaat gggaaagatg cagtctacac gatcaaagaa gaaccaaaag
     5941 acggagtgtc agctgccgca aacatggttt tagctttccc tgtatatgag atgatcaaac
     6001 aagcagatgg ctcttataaa tacgggacag aagaactaga tactatccat ctctacccta
     6061 aaaatacagt cggtaatgat ggaacgttga aagttacaaa aatcggtact gccgaaaacg
     6121 aagcactaaa tggagcagaa tttattattt ctaaagaaga aggaacacca agcgtcaaaa
     6181 aatacatcca aagtgtcaca gatggattgt acacttggac aactgatcaa accaaagcca
     6241 aacatttcat tactggtcat tcttatgaca tcggcaacaa tgactttgcc gaggcatcta
     6301 ttgaaaaagg ccagttgatc gttaatcatt tagaagttgg aaaatataat ttagaagaag
     6361 taaaagctcc tgataatgcg gaaatgattg aaaagcaaac aatcacgcct tttgagatcc
     6421 tggcaaatag ccaaacacca gtagaaaaga ccatcaaaaa tgatacgtct aaagttgata
     6481 aaacaacacc tcaattgaat ggaaaagatg tcgcaatcgg tgaaaaaatt caatatgaga
     6541 tttctgtcaa tatcccatta ggtatcgctg ataaagaagg aacgcaaaac aagtacacaa
     6601 cattcaaact tatcgatact catgacgctg ctttaacatt tgataatgat tcttcaggaa
     6661 cgtatgctta tgccttatat gatggaaata aagaaatcga cccagtaaat tattctgtca
     6721 ctgagcaaac agacggattc acggtttcag ttgatccgaa ttatattcct tcattaactc
     6781 ctggcggtac attgaaattc gtttactata tgcatttgaa cgaaaaagca gatccaacca
     6841 aaggattttc taaccaagca aatgtcgata acgggcatac aaatgatcaa acaccaccgt
     6901 cagtcgatgt cgttactggg ggcaaacgat ttgttaaagt agatggtgac gttacatcag
     6961 accaaacact tgctggagca gaattcgtcg ttcgtgatca agatagtgac acagcgaaat
     7021 atttatcgat cgacccatcc acaaaagccg tcagctgggt atcggcgaaa gaatcagcaa
     7081 cggtttttac aaccacaagt aacggtttaa tcgatgtgac aggtctaaaa tatggcacgt
     7141 actatctgga agaaacgaaa gcgccagaaa aatatgttcc attaacaaac cgtgtagcat
     7201 ttactatcga tgaacaatct tatgtaacag caggacagtt gatttctcct gaaaaaatac
     7261 caaataaaca caaaggtaca cttccttcaa caggcggtaa gggaatctat gtgtatatcg
     7321 gtgcaggagt agtccttcta ctgattgctg gactgtactt tgctagacgc aagcacagtc
     7381 agatttagtc tttttgcaag aattggaaaa aggagcagcg gattacgcaa atgctccttt
     7441 ttcttttatt atcaaacagt catcacaagg agaacacaaa tgaaaccaaa gaaaaggaaa
     7501 cttctagatc tgctgatgct tttcttgctt ctctcaggaa tagggatact agcttatcca
     7561 tttgttagcg acgcgctgaa taattatctg gatcagcaga tcatctctca ttatcagcaa
     7621 caagcggtca aagaaaatga agaagtcatg gctaaaatcc aagaaaacat gacaaagaaa
     7681 aaccagcaat tagcaaaaga aggtggaaat ccaggtgcag atccctttac taagaaaaaa
     7741 gaacctgcga aaaaggatag aacctatttt gagaaacata cgatcggtat tttgacgatc
     7801 cctgagatca atgtccattt gccaattttt gatgaaacga atactgtgct gctagaaaaa
     7861 ggtgcctcat tattggaagg aacgtcttat cctacggggg gagaaaatac acatgccgtt
     7921 atttccagcc atcgaggact gcctcaagca aagttgttca cagacttacc gaaactgact
     7981 ttaggtgatt ctttctatat cgaaatcaat ggacggacac tcgcttatca agtcgatcag
     8041 atcaagacgg tagaaccgac tgatacaaaa gatctgagaa tcgaaaaagg gcaggatttc
     8101 gtgacactcc taacttgtac accttatatg gtcaacagtc atcgcttact tgtcagaggg
     8161 caccgggttc cttatcatgc aaaagaagtt caaaaagaag tacagaaagt atccgagaac
     8221 aaacgatttt tcctgtatgg attgttagct gcatgtatac tgattttcct ccttcttata
     8281 tatctttttc gtcaatcgtt caaaataaga aacagaagtt agctattcct catttctata
     8341 atatatctat tcgaatcgtt ccgtaaacaa acaggctgag gtttgaaaaa gaaaacttca
     8401 gcctgttttt atgatgatat caaagagagc ctatcaaaca ctatctttct tattactaaa
     8461 tgaggatctt ttcttaagtt ttccgatatt ctttaagaag gcctttttgc atcaattctt
     8521 gtttgttttt caagttgcgg atatt
//
LOCUS       DACEMO010000114         8419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEMO010000114 DACEMO010000000
VERSION     DACEMO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.117"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            11..838
                     /locus_tag="IW728_001921"
     CDS             11..838
                     /locus_tag="IW728_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025271.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(1111..1881)
                     /locus_tag="IW728_001922"
     CDS             complement(1111..1881)
                     /locus_tag="IW728_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1025272.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            2099..3166
                     /gene="ulaG"
                     /locus_tag="IW728_001923"
     CDS             2099..3166
                     /gene="ulaG"
                     /locus_tag="IW728_001923"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1025273.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            3184..3645
                     /locus_tag="IW728_001924"
     CDS             3184..3645
                     /locus_tag="IW728_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1025274.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            3665..5149
                     /locus_tag="IW728_001925"
     CDS             3665..5149
                     /locus_tag="IW728_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1025275.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            5192..5491
                     /locus_tag="IW728_001926"
     CDS             5192..5491
                     /locus_tag="IW728_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1025276.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            5506..6144
                     /locus_tag="IW728_001927"
     CDS             5506..6144
                     /locus_tag="IW728_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1025277.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            6149..7009
                     /locus_tag="IW728_001928"
     CDS             6149..7009
                     /locus_tag="IW728_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1025278.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            6999..7709
                     /locus_tag="IW728_001929"
     CDS             6999..7709
                     /locus_tag="IW728_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1025279.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            7941..8375
                     /locus_tag="IW728_001930"
     CDS             7941..8375
                     /locus_tag="IW728_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1025280.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT         2775 a         1321 c         1806 g         2517 t
ORIGIN      
        1 ggaaattgtt ttgattaaag tagatggagc attactaaaa gaataccgaa agaaaaaggg
       61 gttaacacaa gtacaattag cagaaggcat ttgtgaacaa gcaactgtca gcaatatcga
      121 aaacaagaat atgtcgccag gcatcaatat cttaagtgcg ctttgcaaaa agctgaatgt
      181 cgagatctcc gatatattag tagaaacaga agaagacaaa atcaggaaaa aaataaatga
      241 gattcaatca ttgaattctg tatcaaaata ccaagaatct tataaaattt tacaaacgat
      301 cgatcaggaa aaagtaagta attctgatct tttaaatcaa attttatttt atcaaggact
      361 gcttgatcat ctttatgcga aaaaaccaga tgcagcatta ttttatttta atcaagtgtt
      421 ggaacaaacg accgaaacag atatccatca cttacaagca gctgcaaacg tggcaatgat
      481 ctacttgtca aaaggagaat tagattttgc gaaagtttat gtggagcgta cacttaaaat
      541 attgagtgag actgattttg ataatctgat ggtctgcatt gtatattatg atatcgccac
      601 ctattaccgt aaaatcgagg attatgataa agcaatccaa ttatgtgaga agggtattga
      661 gtataataag aaacataaat cgacttatgc attggaatac ttgctttatg aaattgcttc
      721 ttgccataag caattaggag aagatgatta tttagaaaga tatatggacg caaaaaaaat
      781 agcgcgtttc aacggaaatg attacgcggt aaaggtgatt gaaaacgacc tgaaataaag
      841 tggttgtgaa atcagtgttc tgatttgaat accgtttatt cgaaaaaagg aggtgtgact
      901 tttgtcatac ctcctttttt cgatattgtt tgattcctat ttaggcaaca tataacggtt
      961 ataaaagaag caatagatta tgatggtatc gattggatgg atacatcttg ggattccttt
     1021 atcaatttga gcgtagcgtg tttttcattt atcgataaat ttgttattca tttttttgat
     1081 aatcctattt acatatttac gtcctggttc ttaaaatgaa tctacttgaa tggtttttat
     1141 ccccgtattt ctgatctcct ctaatacttt ttcagaacaa aatttatcag tgatcaaata
     1201 atcaaagact gaaatatcag caatcttgaa ctttgatgtc agtccgattt ttcgatgatc
     1261 agcgactaaa atcttacatt tgattgtctt gttgaccatc atttcgttga tcgttgattc
     1321 atttaatatc tttgttgtga ccccatgtac tacatcgact ccgctacagc caatgataca
     1381 aatgtctgca ttgactgaag aaatcgtatt taatgcgatg tcgcccacta gtgcttcttt
     1441 tggaaagcgc atctctccac cagttaaaat ataactacaa ttatctttgt gcggagagta
     1501 attgattttc acgttgtttg taatgactgt caaatggtta gattgaagaa aatccagtgt
     1561 tttcaacgca gtcgagctag aattgataaa cagcgtatca taatcgttaa tgtatttact
     1621 agcttctctg gcaatcgctt gtttaatcct ttcaggacca gattgatcaa aatcagtctg
     1681 gtttttgtaa ttataagaac aataaccgta attgcgtatg atctcgtttt tttcttctaa
     1741 taaatttaaa tctcttcgta tcgtactaat agatacatca agggattcag ctaattcgaa
     1801 agttgatagt tgttcagtct gctgaagcag ttccaaaatt tctttttgac gtttttcaat
     1861 attgacaagt gagtttttca tgacttttgc ctcctgaggg acatcttgat ccatggattt
     1921 atttgactag ttgttcattt aaacattcat atctgtcttt attatactga aacaatcatt
     1981 gattactgaa agaaaatcat ataaaaaaca aacggtcata taaaagggtt ttcatggatg
     2041 ctgatacaca ttaatattta cttgtaagtt ggaacaacga tatgggggga gcaagcaaat
     2101 gagtaaaact gtcaatgaag taacaaaaga aaagtggtta gtagaaacat ttcctgagtg
     2161 gggcacatac ttgaatgaag aaatcgaaac agaagtagtg gatcctggaa cttttgctat
     2221 gtggtggctg ggctgtacgg gaatttggct gaaaagtcat gaaggaacga atattttatg
     2281 tgatctatgg tgtgggacgg gaaaacgtag tcatgggaat ggcatgatga aaaaaggcca
     2341 tcagatgatg cgtatgagtg gcgttcaaaa tatgcagcct aatttacgta ctcagccttt
     2401 cgtaatcgat ccttttgcga taaaaaatgt agacgcttta atcgttacac atatccattc
     2461 cgatcatcta gatatccata cagccgcagc agttcatcaa aattgtcctg aagcaacatt
     2521 cattgggcca aaagaagtgg ttcgcacgtg gcaaaaatgg ggaattcctg aagaaaaaac
     2581 gattacagta aagccaggtg atgcagtcaa aatcaaagac atcaatgtag tagctttaga
     2641 agcgtttgat cggacagcgt tggtcacttg tgaagatcca gaagaggtat taaaaggaaa
     2701 aatgcctcag gagatggatc aaattgcagt aaactattta ttcgaaacat caggagggaa
     2761 tctttatcat gcaggagatt cacattactc caatttattt gccaaacatg gtaatgaaca
     2821 caaaatcgat gtctgtttag gggcctatgg tgaaaaccca cgagggataa cagacaaagt
     2881 tacttctgtt gatatgctgc gcatggcgga gtcattgaat gcaaaagtag tgattcctgt
     2941 ccactacgat atttggtcta attttatggc tgatccaaaa gagatcactg aaatttggaa
     3001 attcaaaaag gatcgcctga agtatggatt taaaccttat atctggcaag taggcgggaa
     3061 attcatctat ccgacaaaca aagatgatct tgagtttaat ttttatcgag gatttgaaga
     3121 tgtcttctcg attgaaaatg atacaccgtt tccgtcgttt ttataagaaa gaaggggagt
     3181 ttcttgctga aatacttttt tgaccatcaa ttgattcatt acccagataa agaaatcacg
     3241 gattggcgtg aagctatcgc cgaaagctgt cttgtattga tcgataagga aattattgat
     3301 gaaacgtatg tggaggagat catccattgt gtagaaaaat acggtcctta tattgtgatc
     3361 gcacctcaag tagcaatgcc gcattcttca gaaaaaagtg atggtgtttt tggaacagct
     3421 atttcattta ccaagttgaa acagccaatc cgttttgaaa gtgaagggga gaatgaggag
     3481 aaaacggcta gtttgttttt tacactcgca gcaaaagatc cagaagaaca tctagaaaat
     3541 atccaaaaat tatccgaatt attaatgaca gatggattgg ttgagcgtct catctctacg
     3601 aactccatcg atgattacca agttgtgatg gcagaatata attaattgat agaggagaaa
     3661 aaagatgggg gaatttttgt taagcatttg gacgtatttt gcaacaaata tcttgacgca
     3721 gccagcatac ctgattggtt ttatcgtgtt attaggctat attttgctga aacgcccgct
     3781 tcatgaatgc atcgctggat tcttaaaggc aacggtcggt tactttattt tgactgttgg
     3841 ctctggtggt ttggtcaata attttcggcc aatcttggtt ggactaaagg aacggttcaa
     3901 tttggatgcc atggttacag atccttattt tggacaaaat gctgtggatg ctggattaat
     3961 ggagacattt ggccgtacct ttggagatgt gatgatttta ttactgattg catttgtttt
     4021 aaatatctta ttagtccgtt ttcagaaata tacgaaactg cgttccgttt tcactaccgg
     4081 aaacgttcag gttcaacaag cagctaccgc tttttggatt ttactgtttt gctttcctga
     4141 tctaggtagg atacaagcat tgatttttat gggactgatt ttaggttgtt attggacagt
     4201 agcttctaac ttaacagtag gaatcacgca ggaattgaca gaaggtgccg gatttgcagt
     4261 ggcacatcag caaatgttcg gaatctttat atttgcaaaa ttagctgagt ggatgaaaaa
     4321 gcgggatgaa aagaaaaata gatcaatcaa gcaagacaaa aaaattgaag atatcaaatt
     4381 accaggattc ttgtcgattt tcaatgagaa tatggttgct acttcgttac tcatgctatt
     4441 tttctttggg atcattctga ttgtactagg caaagattat ttgattcaag cgcaattcat
     4501 gcaagaaggg cagagtttct tattctacat tatgacgact tctttaaatt ttgcggtata
     4561 tctggcgatc ttgcaattag gtgttcggac attcgtcgat gaactaactc aatctttcca
     4621 aggaatctca aacacgattt taccaggagc tgttccagga attgacgtag ctgctacttt
     4681 tggctttggt tcgccaaatg cggtaacgat tgggttcttg tttggtgctt tgggacagtt
     4741 tttgatgatt ggtttactga ttttattgaa atcaccaacg atcgtcatag ccggtttcat
     4801 tccgttgttc tttgataatg ccgttatagc agtttatgcc aataaccgag gtggattcaa
     4861 agccgcttgc ctgttcccgt ttctttcagg aatcatacaa gtaggcggtt cagcattatt
     4921 cgctacatgg attgggatgt caacatacgg tggttatatc gggatgttcg actgggcaac
     4981 agcttggcca ttgtttacag taatcatgaa agtcctaggt tatgctggaa ttgccgtagt
     5041 tgtcattggt ttattggcta ttccgcagtt acaatatcgg aaaaatcctg atggttattt
     5101 tttgattgta gaagactatg acacatatgc agaaaagcag agagtataaa agaaaatctg
     5161 attagacaac actcataatg gagggattaa gatgagaatt ttagtatcgt gtgcaaatgg
     5221 atcaggaaca agcttgatga tgatgagaag tgtggaaaaa gcaatgaaag aactaaacgt
     5281 accaattacc aaaatccatc attgtgccat ttcagaggga aaaagttcag ctggccaata
     5341 cgatgtagtt tttacgccag tcaatttttt gaatatgttt actcaggcag agaaaaaagg
     5401 agttacagtt atcggtatca aaaatgtgat gtcagtcaaa gaaatcagtg aaaaatttgc
     5461 tgaaacaaag tggtaccaaa aaaacaagta aaggaaggaa ctaagatgag caggccgaat
     5521 ttgcaaattg cgttagatca caatgactta gaacatgcat taggagatgt tatgaaagtt
     5581 ggtgatgtag tagatattat tgaagtgggc acaattcttt gcttacagga aggacaaaaa
     5641 gcgattcgct gtattcgcag tatgttccca gataaaaaac tagtcgctga tacgaaatgt
     5701 gcagacgctg gaggaacagt ggcgagtaat gttgcaaaag caggagcaga ctggatgaca
     5761 gtcatttgtt gtgcaacgat tccaactatg gaggcagcac aaaaagaaat aggtgaattg
     5821 caagtagaac tctacggcaa ttggactttt gaacaagcca tggattggca taacatcggt
     5881 atccggcagg taatctacca tcaaagccgt gatgcgctgt tatctggtga aacttggggt
     5941 gaaaaagatt tatcgaagat caaaaaactg attgaactag gctttaatgt ttctgtaact
     6001 ggtggattaa atccacacac attacactta tttgaaggga tcgatgtgta tacctttatc
     6061 actggtcgtg gtattacagc agcaaatgat ccgatgaagg ccgcacaaaa cttcaaagat
     6121 gagatcattc gtatctgggg gtaaggaaat ggctcaaatt gggatttatg aaaaagcgtt
     6181 acccaaagat atttcatgga aagaacgttt ttcccttgta aaagaaatgg acttcgattt
     6241 tatcgagatg tcgattgatg agacggatga gcggttagca cgattagatt ggtctgaaga
     6301 aaagatagct gagctgagag aagagatgtt cagctccggt gtacgtatcc attcgatttg
     6361 tttaagtgcg catcggcgat ttccgtttgg ttctgctgat ccagaaaaga aaaaagcagc
     6421 gaaacaacta atgaaaaaag caatccattt ggcgcataat ctaagcgtca aagtaatcca
     6481 aatagctgga tacgatgttt attatgaaga gaaatcaatg aattccagag aagactttat
     6541 cgaaggaata aaagaaagcg taaaggaagc ttctaaatat ggcatcattc tttccgtgga
     6601 aatcatggat gatccattta tgaactccat ttcaaaattt ttagaaatca aagaacagat
     6661 tcattcacct tttctacaag tatatccaga cctaggcaat ttgtccgcat ggccggaaaa
     6721 taatccggct cgagaacttg agaaagggat cgattatatc actgctatcc acttaaaaga
     6781 tacttaccct gtaacaaaag aatattcagg ccagtttcgt gatgttcctt ttggtagcgg
     6841 ttgtgttgat tttcttggat tattaagaca tttgaaacgt ctagattatg atggaacatt
     6901 tctgattgaa atgtggagtg aaaaaagcca agactttcga agagaaatcc agcaggcaaa
     6961 agactactta agttctaaat tgaaggaggc tggctatgat gtcttgtgaa gaaaaaattg
     7021 cagcaatgaa gcaacgtgtc cttgaagcaa atctggcatt gccgcgatac ggattagtca
     7081 aattgacttg gggcaatgtg agtgaagtgg accgtgagct aggagtcatc gtcattaagc
     7141 caagcggtgt cagatatgaa tgcatgcaag cagatcaaat ggtggtaaca gatttgtcgg
     7201 gaaatataat agaagaagat agcttaaaac catcttcaga tctccccaca catgttgttt
     7261 tgtatcaaac ttttgaagat ataactgcta ttactcatac tcattccacg cactccgtca
     7321 tgtgggcgca agcaggacgc gatttgccag cttatggcac gactcatgct gatgcttttt
     7381 atggtaaggt tccttgtaca cgccaattaa cgaaagaaga agtaagagaa gcttacgaag
     7441 ttcatacagg gaatgtgatt gtcgagacgt ttaaggaaag aaaattagat ccaaacgaag
     7501 tccctggagt acttgtctat ggacatggac cgttcacatg gggggattca cctatgaaag
     7561 cagtagaaaa cagtttgatc ttagatgaaa tttgtctgat ggccaaagaa aacgagctga
     7621 tcaaccccaa tatatgtgaa atcccgcaat atttactaga taaacattat tttcgcaaac
     7681 atggaaagta tgcatactac gggcaataga gatgctaggg taagggtaga aaattaaaag
     7741 ataacacttg atagagagaa acagaggttg tgaagaaaat ttatcacaac ctttttattt
     7801 atcaaaaagt atgcattcta aaaggaaaga ggaattttaa ggaatcgaga gaatctttac
     7861 aggagagtta tatgtgatag attaaatagt caaagaactg tattattaga gggagaattg
     7921 tataagaaag gaagaaaaaa atgaatattg attcccttat gatcgatgag atttctgaat
     7981 tatataagag cgttggttgg acgaattaca caaaagacac tgcaaggtta gctagagcat
     8041 ttgaacaatc tgaatctctg ataaagcgta atggggaagg gaagattatc ggaatcgttc
     8101 gttggatcac tgattttgcg acgatcgcat ttattcaaga tatcctcatt catccgaggt
     8161 atcagcgcca aggaataggc aaagtgcttt tgaatgaagc tttggagaag atcactagtt
     8221 atggaccagt ccaaatcgaa ttattgacag atgatactga gaaaacaaaa aaattttatg
     8281 aatcagtagg atttgttcaa gtaaaggaaa tgggtggtat ttcttatgtc atagatacta
     8341 gaagaaaaaa ggtgcttttt ggtaatgaca aataacatat aataacgtac aaaagaaaaa
     8401 attctagctt agttcttta
//
LOCUS       DACEMO010000115         8377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEMO010000115 DACEMO010000000
VERSION     DACEMO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.118"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..199)
                     /locus_tag="IW728_001931"
     CDS             complement(<1..199)
                     /locus_tag="IW728_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1025281.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQD"
     gene            complement(265..1554)
                     /locus_tag="IW728_001932"
     CDS             complement(265..1554)
                     /locus_tag="IW728_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1025282.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(1641..2345)
                     /locus_tag="IW728_001933"
     CDS             complement(1641..2345)
                     /locus_tag="IW728_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ1025283.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            2545..2811
                     /locus_tag="IW728_001934"
     CDS             2545..2811
                     /locus_tag="IW728_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025284.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(2979..3209)
                     /locus_tag="IW728_001935"
     CDS             complement(2979..3209)
                     /locus_tag="IW728_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1025285.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            3281..3436
                     /locus_tag="IW728_001936"
     CDS             3281..3436
                     /locus_tag="IW728_001936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025286.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(3745..3969)
                     /locus_tag="IW728_001937"
     CDS             complement(3745..3969)
                     /locus_tag="IW728_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1025287.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            4236..4571
                     /locus_tag="IW728_001938"
     CDS             4236..4571
                     /locus_tag="IW728_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025288.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            4804..5745
                     /locus_tag="IW728_001939"
     CDS             4804..5745
                     /locus_tag="IW728_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1025289.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            5747..6637
                     /locus_tag="IW728_001940"
     CDS             5747..6637
                     /locus_tag="IW728_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1025290.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            6680..7480
                     /locus_tag="IW728_001941"
     CDS             6680..7480
                     /locus_tag="IW728_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1025291.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7495..8346
                     /locus_tag="IW728_001942"
     CDS             7495..8346
                     /locus_tag="IW728_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1025292.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
BASE COUNT         2862 a         1397 c         1582 g         2536 t
ORIGIN      
        1 catcttgtaa tagtttttga aattcatcac gatttatttt ttgaccagaa ataccaccgt
       61 ctacatattc attaactact ttcaaatcct tgtgttgttc gatatattca tagagggttt
      121 ctttttgttc tctaagacta tctccatgtt tggcttgttg ttcagttgat actctcatat
      181 ataatgctac tcgtttcatg ttatctcctt tcgaatgtat gttcttatcg ttgtaaaaga
      241 aaagcccgaa ggctaatctt tttactacac atctgttaat tctaatgatc ttctgtattg
      301 ttcagcttga gctagtttag taaaagatac agttccgtta taattttcta aaacaacttt
      361 ttcaatttca tcaagagata ctttaaagaa ttcttttcgt tgattaactt tattgacttc
      421 gttttttcta aaatgcgaat gtagttgatt ttctaatgtt ggtgcatctt ctgaaaatat
      481 cattgcatga acatcaaatt caaaaggtac agaagcgcta ctaagttcct taattctatc
      541 cataggttct aaccgtctag tcattcctat tttatagaca tcttcaccga aagatccgat
      601 attactgatg acataaacat agccagctct agtattaagt tctctttgca atacatcttc
      661 tttgacagtt tctagttctc ttaatttctc ttccagttct ttaattttat cagcatagag
      721 ttctttttca acatcagcac tagctttttg catgtatgcc attaacttag ttatctctga
      781 gttaaactgt cgttcttctt tatcaatgcg tttcttttca cgttctattt cacgtcttac
      841 cttttcttct tcaatcattt gttcttttat tgctttttgc tgttcttttt catcgttaac
      901 tttaactatg tatgaatagt tgagttctaa ttcttgaagt tttaattcaa gtaattcttt
      961 tttgaattct gcttcatcgt ttttaaaaag cttgtttatt ttatcaaaag ttcttataat
     1021 tttttctcta gttgaatcta cgttagaaat agtaagttta ttgagaagtc cagttatttc
     1081 tgattctaat ggtataagca ttttcttttt aatattatta gcttctctat tattcaaata
     1141 agaaggaata ttaattgcac cgtctaaaat aaaatccttt tctttaatct ttaataaaga
     1201 aagtttgttt tttatttcgg atgaagaagt ttcatctata ttcggaataa ttactgattt
     1261 atagatagtg accagagatg tgtcatattt ttgattaatt tcatcgtcta accgctggct
     1321 ttcagcatta agaatatcaa ttttgttttc tagcgttgac aagtttttat tcaatttact
     1381 tatgtttttc tctttttcag atataaacgt ttccctagca tttaattctt gagaatattc
     1441 ctttacctta ctctctaagg cagaagtttt atcttttaat tttttagctt cagataattt
     1501 gctactaccc ataattatta aaactattcc aactaaaaac aatagtactc ccattcaaaa
     1561 cgcctcgctt cttttataaa ataattgtca aaatcatata aacccttacc gaagttgacg
     1621 gctccggcat gggaattttt ttaaaataca acttttccga taattctaat ctgttctggc
     1681 gatacaattc tatcttcgta taagtcgttt atagaacgta aaatgattgt ttgattttcg
     1741 taatctttat atatctcttt acatgtaact ccgtcaccac ctaattccac aatagcaatt
     1801 tccccatctt ctacgtccgg ttgttcttta acgaatacga attgtccatt cctgataaca
     1861 ggttccattg aatcgcctat aataggtact gcgaagtcag cagcacttgg aactctctca
     1921 aattcttcct cttcgacaac cgtatcaccg taagtaagtt ctgttggatt ggcagccgaa
     1981 gccgaattgt gaacagttgg gattttttct tttttaggaa aatctaggac attttcttgt
     2041 ttttgttctt ctaatcgatt acttgcaaat tcataggtag caactttatt ttcgtcgtta
     2101 agctgattat atataggaag gatattatat atctgattag atatagataa atcaatattc
     2161 atcaaatcat ctatatcaac attgaatatc ttagctatct catttaaggt ttttaatttt
     2221 ggtgtatatt ttcctttttc ccattcgctt attgaggatg aactttttct tcctagtttt
     2281 tcggctagtt cttgttgatc aatattatac ttttctctaa gaaactttag attttcagcg
     2341 aacatagtaa cacctccttg agttaattgt aacaccgttt cggaaaaaat gtaacaggtt
     2401 tcttagaaaa tattttagtg tttttaaaga agtatttacg caaaatagaa atcgatttca
     2461 ggaaaactga aatatgcact tgacttcgga aaaaccgaag tatataattt agtcagaaag
     2521 ttaaagagag gaggcgaata aaatatggat accgcgaaga aagaaaaata tacattgaga
     2581 caattaagag ctttaaaggg ttattcaaaa gaagaactgt caaggaaaag caaagtgaca
     2641 tcaagaacaa tatttattta cgaaaacgat gtagataaaa tgaggaacgg aaagtacgcc
     2701 actctagaaa aaattgctaa ggcattgggt gttagagttt ccgatatttt tttagaccct
     2761 gattcggaaa aaccgaaatc aataacaggt gaaacaaaac aagtcagcta ggaggtgtga
     2821 atagtggaag agaagaatat aaaagtaccg actcttgaag aatatacaaa aattgttgtc
     2881 gaaacagacg agcagaaccc gaaaacaatc gcagttatta cggcgaatga tattgaatca
     2941 tgcgaaggtt ttcgagttcg aatgacccct agatatgatt aatgttcttt atcacgtgga
     3001 ggtataggat cgtttccaaa actatctttt gattgtattt gtccattctt tttttggaca
     3061 ataaactctg attgctgttt tttagaaatc tttttaccat actcaacagc atctgattta
     3121 ttatcaaata gttttgtatc ttcggaatta ccttcaccac gtacagccca tttgccattt
     3181 ctaggagata cccattgatt tttacccata acaattcacc tcgctttcaa gttaattcta
     3241 ttaagaggtg aaaagaaaac aaactgatgg aggtgagatt gtgagtaaca agccaaaaat
     3301 cactttgaat ggcacaccta atcttcaaag attagcagat gaaatggcta gaatacgttc
     3361 tagacattgg ggagttactg tggtggcaac cataattgaa caaaaagaaa agcccacaca
     3421 gccggcaagc aagtagtggg caccatagaa caataatatt atatcacaca ctcgccactt
     3481 ccgaaaagag gagttgctgc tctcctttca agtttgccaa agtccacgtt tcttcatttt
     3541 ggcaactcct cttcttggag gtggtgacgg cgagagacga aggagggatg acatgaagga
     3601 agaatcaaaa atagggattt tgaatgagaa caattacaca agattaatta ttgaaacaga
     3661 agaaggggaa aaagtagcag aagttacatt gaccgatgca actcctgctg acggttatcg
     3721 tattcgatta actcctaaat atgattaacc ttttggtggg tgtggatcgt ttccgtgact
     3781 atcacggctt tgaatttttc catctctgcc atgaataaat agctcagatc cttgattttt
     3841 agaaatctct ctagcgattt ttactgcttc tgattttgta ttagtatgtg cagtcgcttt
     3901 agaatttcca gcacctttga cattccaacc accattggaa ttaggtacaa catgttgatt
     3961 ttttcccata gttttcacct ccatttctat agaaatattt tatcaaatag aaatggagaa
     4021 attcaataaa cgaaaggagg gatcaaatga cattacaaga gcatcaacac ttgatgcgaa
     4081 aactaaatca ggaataccac gaggaaatga aacctagatt gctaggggaa caatggcgtg
     4141 aacgccagaa gaagtggtta gaaataaaaa agtgactcag ccgtccaaag caatgagtca
     4201 caaaccaaaa attataccta aggagatttt atcacatgaa taagaaaatt gaaaatctta
     4261 tcgaagaatt gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt acagcccaaa
     4321 aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta ctttgtcttg
     4381 ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt atgcgtagaa
     4441 ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct tcaaatcata
     4501 cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt ctcaaggggg
     4561 aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt tcacgttagt
     4621 gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca tgcttgtgct
     4681 ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat cttgacaact
     4741 atttaactga tcctgattat gctaaaccac catatgaagc gccaattgat gaggaggatg
     4801 aagatgagta aatctacctt agaaatgagc catcaagaat ggctcgaaga ccgcaaaaga
     4861 ggtattggtg gctcagatgt tgcaaccgta cttggattaa acaaatacaa atctccttat
     4921 caattgtggc ttgagaaaac gggtcaaatt gaattgaaag atttagaaag cgaaccagct
     4981 tattggggta atgttttaga agaagtggtt gctaaagagt ttcaggaacg tacaggcaaa
     5041 aaagtacgca gaagaaacca agtatttgaa catccattac atccattttt aagagcaaat
     5101 attgatcggg aagtagtggg agaaaatgcc attctggaat gtaaaacagc caatcaattt
     5161 ctcggaaaag agtgggaagg tgaagaggta ccgcttagct atctctgtca agttcagcat
     5221 tacatgaacg ttttaaacaa agactattgt tatatcgctg ttttgatcgg tggacaaaaa
     5281 ttcatttgga agcggattga acgagatcaa gagctgatcg atacaatcac tgaacaatta
     5341 gtagagtttt gggaaacgaa cgttcttgga ggtatcgagc ctgtaattga cggaagtgga
     5401 gcgactgctg acttcttaaa agaaaaatat gcagatgtag aagaaaatca aacagctcta
     5461 ccatcacgtt ttgatgaact tatcgagcaa aaaagagaac tcaagcggac gaaaaaagaa
     5521 attgaatcat ctattcgtca agtagacaac gagatcatca gtgagctagg taaacgtgaa
     5581 gccagtatcg gtatcacaca aagaaacatt atcagctgga aacttgttcg tacaagacgg
     5641 atgaacacga agaaactagc agagaaatat ccagatgtcg caaatgatga agagatttat
     5701 aacgttactg aatcaagaag gctaaccgaa aaggagatca aataatatgg caacaaatga
     5761 atcgttaaaa aatcaattgg cagaaaagcc acagaaacaa gttgcaccag gacagttagg
     5821 gcttaaagct ctaatgaata caccaacaat gagaaagaaa tttgaagaag tacttcatga
     5881 caatgctaat gcttttatgt cgaatgttat gactcttgta tctaatgaca gttatcttgc
     5941 agatagtgaa ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat taaatcttgg
     6001 gctagataag aatttaggtt atgcatattt agttccattc aatagtaaaa acaagcaaac
     6061 aggaaaatgg gaaaagaaag ctcaattcat gcttggctat aaaggatata tccaattagc
     6121 ccaacgatca ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag gagaactaaa
     6181 aagctggaac cgactgacag aagagtttga gtttgatcca aatggtagaa cgtctgatga
     6241 agtcattgga tatgttggct atttcgagtt actgaatgga ttcaaaaaaa ctgtctattg
     6301 gaccaaacaa gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg ataagacaaa
     6361 gttaagtggt gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg ttttgagaaa
     6421 tcttctttct aaatggggaa tcttgtccat cgaaatgcaa gaagccacca catcggatga
     6481 gagagtccaa agggttcaag aagacggcag cattattgct gaaacagaag ttgaagaaga
     6541 tattcctgaa agaaaagaag cagaggttat atctgaagaa aatgaagatg tacaaactgg
     6601 attatttgat gcatctaatc cgccgttaaa caaataatga gggagctttc tcccttgctt
     6661 ttctagagga gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa aagtgatgaa
     6721 ttcattcgtt tggaccaatc cttatatgta taaattatgg agcttgtgtt tgatgaaagc
     6781 gagtcatgag aatcgcaaga tactttttaa tggaaaagag atacaagtga acagcggaga
     6841 attcgtcaca gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga aacgtgaaca
     6901 tcaagtgaac agcggttctg tatggagatg gctaaaacaa tttgaaaaaa acggaatgtt
     6961 gaacatcaaa tcaactacga aatacagcgt tatatcaata aaaaactggt ctttgtacca
     7021 aagcagtgaa caacaaatga acatcaaacg cacaacaagt gaacaacaag tgcacacaaa
     7081 caagaatgaa aagaattata agaatgaaaa gaataataaa acatcatcgt cgcaaccacg
     7141 caaaaagcgt gtttacgaca ccgactcagt ttactacatt ctcgcggagg agttattcaa
     7201 acagatttgt cagaatcagg aaatcaaaaa gccgaatctg caaagttggg cagataacat
     7261 tcggaaaatg atcgagatcg acaagcggac cgaaaaccag gtacggggaa tgattgaatg
     7321 gagtcagcac aatgtgtttt gggcatcgaa tattttatct gctaaaaaat tgcgagaaaa
     7381 atacgacaca atggcagcgc aagcaaatcg tgattataaa acaaaacaaa ctaaaacgct
     7441 tgaatacgag aaatttagca cagatgagtt gcctatttga gaggaggcgt aggcatggaa
     7501 actgttgggg aaatcatgga aaagctgata cagaaagtgc ttgtccaacg tggcgaatgt
     7561 cctgaatgcg gacagccttt gtatggatgg cgtacgaaga accctgatgg ctcagaacgt
     7621 tgtaagccaa cctgtatgca atgtgggtat aaagcattgc gagttcagga agacttacaa
     7681 acagaacgaa tttacaacga gagcctgaaa gcaagagcaa tcaatttttt caaaggtggt
     7741 tctgttgtgc ctaatcaagc gttgtttgat tgcacattgc agaattatca aattgtcgat
     7801 caagaaacaa gacaagcggt tgaagtaacc aaacgctttg ttaattcagt cttgttagga
     7861 aatccaagtc accttgtttt aactggaaaa caaggaacag gtaaaagtca cctagcgatg
     7921 gcagcggctt gggaagtctt gaagcgatca aactacgata aaaaaatctt atttatcggg
     7981 ttacaggaaa tgctggatca aatcaaattt tcgtacaaca atcctgaact cagaaaaacg
     8041 attgagggat cgttgattgc agatatcaaa acagcggatt tagtcatcat tgatgatatc
     8101 ggttcagaac taggaaaaga tgcatcagat agtcgagcgt ttggcataaa cacgctaaat
     8161 tcgttcttgg atgcacgaca gaacctagca acgattatca caacgaactt gcttggtgaa
     8221 gaactgaaaa aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa ctctgatgga
     8281 tttacgatgg tattttctca aacagcagac aagcgcataa aaccagtgaa aggtagtatc
     8341 gcatgaataa ataccgtaat aaaaaaactg ttcatcg
//
LOCUS       DACEMO010000116         8326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEMO010000116 DACEMO010000000
VERSION     DACEMO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.119"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            264..1457
                     /locus_tag="IW728_001943"
     CDS             264..1457
                     /locus_tag="IW728_001943"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1025293.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQIAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1587..3059
                     /locus_tag="IW728_001944"
     CDS             1587..3059
                     /locus_tag="IW728_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1025294.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3142..3930
                     /locus_tag="IW728_001945"
     CDS             3142..3930
                     /locus_tag="IW728_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1025295.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3997..4665)
                     /locus_tag="IW728_001946"
     CDS             complement(3997..4665)
                     /locus_tag="IW728_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1025296.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4780..6492
                     /locus_tag="IW728_001947"
     CDS             4780..6492
                     /locus_tag="IW728_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025297.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6495..8267
                     /locus_tag="IW728_001948"
     CDS             6495..8267
                     /locus_tag="IW728_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025298.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2549 a         1389 c         1824 g         2564 t
ORIGIN      
        1 gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta aaaagcttag
       61 cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa tgtaaaatat
      121 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
      181 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      241 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      301 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      361 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      421 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      481 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      541 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      601 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      661 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      721 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      781 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      841 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      901 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      961 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
     1021 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
     1081 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1141 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagatagctt
     1201 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1261 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1321 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1381 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1441 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
     1501 ctttttttat ggcagttaac ctcttgaaaa agatagtaat tatttatcta tttttagatc
     1561 ctgtcataat gaaagggagg aataagatga aaaaagaaac aaatgttact ttggtcacag
     1621 tgagtatttt cattgcaacg ttcatgacgg ctattgaagg tacaatcgta acaaccgcta
     1681 tgccaacgat tgtcggttca ttacatggaa tggaaatcat gaactgggta ttttcgattt
     1741 atttgctgac aaacgctatg cttactccga tttacggaaa acttgcggat aaagtgggac
     1801 ggaaaccaat ctttattttt gggacattat tgtttattat cggttcgtta ttttgcggtt
     1861 tatctcaaag catgattggg ttgattgctg ctcgtgcgat tcaagggatg ggtgctgggg
     1921 caatgatgcc agtagctttg acgatcatcg gcgatcttta ttctattgaa aagcgagcaa
     1981 aagttttagg tttgaatagt tcagcctggg ggattgccag tgtttttgga ccgcttgcgg
     2041 gaggattcat cgttgaaacg atcagctggc actggatctt ttttatcaat gtgccgatcg
     2101 gaattctatt aattattttg atttggctat atttaaatga accaaaaatc atacatgagt
     2161 ctaaaccaat ggatattttt ggtagtttta gtttgatggc catgcttgtc tcattgctgt
     2221 tagggttcca attattagga gacgaaggaa tgagtccgct tgtttctgct ttgctgctag
     2281 gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa aagagctaaa gatcctgtca
     2341 tcatgatgca tttattcaaa aatccactat ttgttgtggt caacttggct gcagcattgg
     2401 ctagtggttt tttgatggga atcgatgttt atatccctat gtggatgcaa ggtgtattag
     2461 ggctgaatgc agcaattggt gggctggtat tagcaccatt atcactagtc tggatggtcg
     2521 gttctttcct tgcaagcaag tggatgactc aaatgaaaac tcattgggtc ttgagaattg
     2581 gactactaat cgcagctatt gggggcgctt ggttgtatct tatgcctttc agaagtaact
     2641 atttatggtt ttttgctata tcaacggtca tcggtatagg cttaggtgtg accatgacta
     2701 cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca gcttggtgta gcaacttctt
     2761 ttaatacgtt agttcgaaca attggtcaaa cagtcatggt ttctattttt ggtttattga
     2821 taaatggggt aactaaacga gaacttgcag cagcaaattt aacaaatgat gcagatatca
     2881 tgaataaact ggtgaatcca caaacggcta aatatattga tgcggatatt ttgttaccat
     2941 taagaaaaat tctttatgat gggttacatt ccgtttatct agttggcttg ttactcgtga
     3001 tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag tggtaaaata aaacagtagt
     3061 ttcggttaag atatgtacgt agccgaatca ttcaaattta aagttattca aaaataactt
     3121 cttactaagg aaggcgaaaa tatggcattt attcaagcaa atatttattc gaatgtgtta
     3181 gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa caaaaaaggt gattggcacc
     3241 agttcagagc aagagacaac ggatatacct gttttatatt tacttcatgg aatgggtggc
     3301 aatcatagtg tatgggaaag acgtacgtcg attgaacgtt atgtagcaaa ctacggatta
     3361 gctgtcatca tgccatctac tgatcttggc tggtacacag atacagttta tgacatgaag
     3421 tattggacat tcattgcaga agagctacct gagatttgtc atgaattatt cccgcaatta
     3481 acaagaaaaa gagaaaaaac atatgccgct ggattatcga tgggcggata tggcgcgtta
     3541 aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa tctctctttc aggcgctgtt
     3601 tctgtaggaa atcgaatgga tgatcttttg atggttcgta aagcatcttt ttgggaaggg
     3661 atctttggac cattggatca aatcgaaggt tcaaaaaatg atccgcaata cttattgaaa
     3721 gaactagtaa aaagcggaaa accagttcca agattttata tggcttgtgg ggaatctgat
     3781 tttctttatc aggcaaatca ggaaatggta tctgctatgc aggaacaagg cttggcggtc
     3841 acctttgaac aaggacctgg cgatcataac tggatcttct gggaccaatg gattcaacgt
     3901 gcattggcgt ggctaatgga agaaaaataa aaaaagagtc ttaaaaagat agctcctata
     3961 gagatcacaa acatagtgat ctctatagga gcttttttac tgctcgtttt ctcttctttt
     4021 attttgttgg cataagcctt caatataggt ttgtgcactc gttagaaaga gtgctttttc
     4081 attcaattct gcatgggact gcatactttc tttaaatccc tctaattgat tttcaactac
     4141 ataattacgc ataaatagaa ctgtttcttt caaaaattca tttccgtcca taattttttg
     4201 tcgaagctgc tgttcatgct gctgatccat taactgtcca atcaataaat tgtggagaga
     4261 ggcaatggcc tgatgacttt gggtgactga aaatccttca cgaacaagta attggatcat
     4321 ttgctccaaa tgttctaatc gactcggaaa agcgggcaca gtattgattt ctaattctgc
     4381 cccacaaggg aaacgtgtat aaaggtcata gtaattttcc atatactgca gcaactgttc
     4441 attccaaggc aaagttggat ctggaagcgc taattcttct tccattgttt cagccatttt
     4501 ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa gcaggtgctt gaacatgaat
     4561 tctgtttgcg atgtttcgca tggaaagctg cgaaagagct tgtgtttcag ccaggatatc
     4621 aaaggcggcc tgaatgatga ttgcttgaga aagctttatt tccattttat caacctcgtg
     4681 tattgcatta tctttttaaa catggtaaat tgttagtcgg tctaatttta cagtgttaaa
     4741 ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa tgaaattaat cttacgacat
     4801 gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt ccgtagcagt catggttgca
     4861 gcagcattat ggcaacctaa gttactacag caagtattgg aagctattat tacagaagat
     4921 aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc tggctttgct aggattagcc
     4981 gcaggtgtaa cgaacacgat tttctctgca aaagtagctc aaggcgtaag tgcagatatc
     5041 cgagaagaag catttcgaaa gatccagaca ttttcatttg gaaatatcga acagttctct
     5101 gctggaaatc ttgttgttcg tctaacaaat gatatcacgc aaatccaaaa tttagtgatg
     5161 atttctttgc aatcactttt tcgaattcca ttcttattta ttggtgcctt cattttagct
     5221 atgatcacga tgccgcaatt atggtggatc atcgtagcgc ttatcgttac ggtatttctg
     5281 attacggcac tttcatttac aaggatgggc aagcacttca tgatcattca aaaactgatt
     5341 gatcgtgtga atagcattgc aaaagaaaac ttactaggga ttcgtgtagt gaaatctttt
     5401 gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa gtgaggactt aacaaaacac
     5461 aacatcatag tcggtacatt attttctgtg atgatcccgt ccttcatgct ggctgccaat
     5521 ttggctgtgg ttggggcgat cttttttgtg agcgatcttg ttaaagatga tcctacgtta
     5581 attggtggaa ttgcttcatt catgaactac ttgatgcaga ttatgatggc aatcattatt
     5641 ggcggaatga tgatgatgat gacttcaaga gcagccgtat cgctgaagcg gattgcggaa
     5701 attttagatg ctgaaccaga tcttacttat ttagacgttc cagaacaaga gttgacgggt
     5761 tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg aggatacaga tacgcttaaa
     5821 gatatcagct tttctatcaa acctggagaa atggttggta tcgtcggggc gactggtgcc
     5881 gggaagtcaa cactagcaca attgatccct cgtctatttg atccgacaga aggcaaagtt
     5941 gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga gtttaagaca aacggtttcc
     6001 tttgttttgc aaaaagcaat tttattctca ggaacgattg ctcaaaactt gcgccaaggg
     6061 aaaaagaatg caacagaaaa agagatggaa catgcttctt ctattgctca agcaaaagaa
     6121 ttcatcgaaa agttatctga tcgttacgaa gcaccaatag aagaacgtag cagtaacttt
     6181 tctggtggtc aaaagcaacg tttatccatt actcgtggcg ttatcggtaa tccaaagatc
     6241 ttgatcctag atgacagtac gagtgcactg gatgctcgct ctgaaaagct ggtccgagaa
     6301 gcactcgatc gagatttgaa gggaacaacg acgattgtca tcgcacaaaa aattgcttct
     6361 gttgtcaaag cagaccgcat cttagtattg gatgaaggca gattagtcgg cgaaggaacc
     6421 catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa tctttgaaac tcaaaaagga
     6481 acggaggagt aagtatgact gatctaataa aagcaagtaa atttttctat cactatttga
     6541 agcggtacaa actctccttt ttctttatct ttatcacagt gatcattgct acctatctac
     6601 aagtaaaggc acctcaatac atcggggaag cttttcaaga attagctaat tacgtaggag
     6661 gcttgatgca aggcgtagac gataaaagca aatttgtttc tgtaatatgg aaactcttgc
     6721 tgttttatgt actagctagt gcagccaact ttatctatag tattttgttc acacaagtag
     6781 tagggaagtc aacgaaccga atgcgtatcg gtctattcaa caaattggaa aaattgacga
     6841 tccgtttctt tgattctcat caagacggtg aaattttaag tcgttttacc agtgacttag
     6901 ataatattca aaatagtttg aaccaagctt tgcttcaagt catcacgaat gctgttttac
     6961 ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga gttagcttgg gctacaattg
     7021 cttccactcc agtagccgtt ttgatagctg tcttgattat ccgcaaagca cggaaatatg
     7081 tggatcttca gcaagatgaa gtggggaaac taaatggtta tatggatgaa aaaatcagcg
     7141 gacaacgtgt catcatcaca aatggcttac aagaagaaac gattgaagga ttcttagagc
     7201 ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc ttattctggt ctactatttc
     7261 cgatgatgca aggaatgtca ctggtcaata cagcgatagt tatctttttc ggcggctggt
     7321 tagcattaaa tggtgatttg gaacgttcgg tggcattagg cttagttgtg acattcgtcc
     7381 agtattcaca acaatattac caaccactga tgcagatttc atctggatac agcatgatcc
     7441 aattagcgat tacaggggca agacgattga atgaaatatt tgatgaaaaa gatgaagtca
     7501 aaccagaaaa cggtaaagca tttgatggtg ttgaaaaagg tcttgcgttg cagcacgtag
     7561 attttggtta ttctccagaa aaactagtct tgaaagatgt ctcgatcaat gtggacaaag
     7621 gagaaatggt tgctcttgtt ggtccaacag gatcggggaa aacgacgatc atgaatctgc
     7681 taaaccgttt ttacgatgtc aatgatggtt cagtagtcat cgatggaata gatatccgag
     7741 attatgatct tgatgcatta cgtagacatg taggaatcgt tttgcaagaa tctgtattgt
     7801 tctccggaac gatcaaagac aatatcacat tcggcaaacc ggacgcttca gatgaagaag
     7861 tcatcaatgc agcaaaacaa gcgaatattc atgacttcat catgacgtta gaaaaaggtt
     7921 atgatacaga gatatccgaa gaaaataact tattcagtac aggtcaaaag cagttgatca
     7981 gtattgctcg aacgatcatc acaaatccat ctttattgat tttggatgaa gcaacaagta
     8041 atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat ggatgaagca atcaaaggaa
     8101 gaaccagctt tgtgattgct caccggctga aaacaatctt gaatgcagat agaatcgttg
     8161 tcttgcgtga tggggaagtc attgaagaag gcagtcatca tgagttatta gcagaaaatg
     8221 gattttatgc ggaactttat cataatcaat ttgtatttga aagttaaatt aaaagaacct
     8281 cgttataacg gggttctttt tatatatttt atgttgtttg ttttta
//
LOCUS       DACEMO010000117         8291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEMO010000117 DACEMO010000000
VERSION     DACEMO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.120"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(114..1082)
                     /locus_tag="IW728_001949"
     CDS             complement(114..1082)
                     /locus_tag="IW728_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1025299.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1314..2147
                     /locus_tag="IW728_001950"
     CDS             1314..2147
                     /locus_tag="IW728_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1025300.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2181..3020
                     /locus_tag="IW728_001951"
     CDS             2181..3020
                     /locus_tag="IW728_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1025301.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3046..3606
                     /locus_tag="IW728_001952"
     CDS             3046..3606
                     /locus_tag="IW728_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1025302.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3805..5526
                     /locus_tag="IW728_001953"
     CDS             3805..5526
                     /locus_tag="IW728_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1025303.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5691..6833
                     /locus_tag="IW728_001954"
     CDS             5691..6833
                     /locus_tag="IW728_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1025304.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6849..8060
                     /gene="thiI"
                     /locus_tag="IW728_001955"
     CDS             6849..8060
                     /gene="thiI"
                     /locus_tag="IW728_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1025305.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2851 a         1438 c         1713 g         2289 t
ORIGIN      
        1 aagaaattta cgaaaattgt ttctcaaatt tttgtaaatt ttcgacttag ttttcgaaga
       61 agctattttt gtcacagcct ctctttttaa gtttagctat tagataatga tgtttagttt
      121 tggataaacg aaatcaattc ttcacccgac aaaccatagt ttacagcaat caatagtttt
      181 aaacgtgctt tttgactatt aatactgtta cagaaaataa tccccatttt ttctagttct
      241 tttcccccgc cttcataatc ataaacaggc tctgcgattc cattgaaaca acgagaaaca
      301 agtactacag ggattttttg gacaagaagc ttttccaaag cagctaaggc ttgtggaggg
      361 agattccccg cacctaaagc ttcgattacc aaaccgtcta ctttcgtatg ggcgatggct
      421 tccaataagt ccccttgcat cccagcatat gctttaacga tcgggatagt cccatctaca
      481 gcggaaatgt ccagtcgctt tgtttctaaa agttcctgca agaataaaat tcgattctta
      541 gttactaagc cgatgggacc caatgtaggt gttcggaaag tagcaacatt tgtggtatgg
      601 gtctttgtca cgtagcgtgc agaatggatc tcatcgttca tgaccaccaa aaccccctta
      661 tctagcgctt cttcacaact tgcgacacga atcgcacttt caaaattata aaggccgtca
      721 cttccaagtt cattacttga acgcattgcc ccagtcaaaa caattggtaa tttgttgcca
      781 atcgttgtat caagaaaaaa agcagtttct tctaatgtat ctgtaccgtg tgtgatcacc
      841 actccggata tacctgatgc ataagcggac aagatacgtt cttttaatac caacatgtga
      901 gaaggcatga tgtgtggtga gggaagattg aagatatctt caactaataa ttctacatgc
      961 ttggggattg tgatttctgc atctaataat gggtttgcca catcaggact aacaccgccc
     1021 gtgagcatgt tttctttcat ggcgatcgtt ccgccggtgt gtagtacaag tatttttttc
     1081 attttacaga cttccttacg aataattttg catagccgtt aaatcttatt gtatactata
     1141 taaaagagtt ttgctatcag tgaagaataa cgaatagcct aacttatcat aaaaaagctg
     1201 accttattaa aatggaaaag actcaacctt gacctcctaa tacaatggag tatctggttg
     1261 agtttctttt acaagaaagc agccaaggaa atcaatcacg aaggtggccg gaaatgatca
     1321 gaatcggatt aacatcgttt aaagaacatg acaaattaac tggtaaaaag aataacactc
     1381 tttatgaata tgcagggttt ctacccttag tggaattaga tactgcctat tatggaattc
     1441 cccgaaagtc ctcagttgag aattggataa gtgaagtacc tgaatcattt cgctttgtag
     1501 tcaaagtcta tcaaggaatc agcggtcaag gagactggaa gccttattat cagaccgaag
     1561 aagagatgat tgcgcatttt ttagatgtta tgtcgccaat ggtagatgct ggaaagatgt
     1621 tttgcttttt agtccaattc ccctcatcat tcaaatgtac gaaagaaaat gtccagtact
     1681 tgcgcaaact tagaaagtgg tttgagggtt atcctgtttc tatagaattg cgggattatt
     1741 cttggtacag cgatgaattt cttgaacgta caagaaactt tatgtctaga gccgcttttt
     1801 ctttagccat catcgatgag ccacagttgc tgaatacaac gattcctttt gatccatttg
     1861 tcaccaatcc gaactttgct ctttttcgat tccatggtag gaatgcgcaa ggatggcaag
     1921 caaatgataa agagtggcgt aaaaaaagaa cgttatatcg ttattcagaa aaagaactgc
     1981 aagaactagc acatgaagtc aaagtagttg attctaagac aaaagaggta ggtgtgattt
     2041 ttaataataa ttctggtggt gatgcggctg aaaatgcatt agcactgcaa aaaatattaa
     2101 atatcaagcc agatggactc aatccaaccc aaatggatct gttttaaggg cttattttat
     2161 tcaagaaagg ggcgaagtct atgatcaaag ctattttttt tgatattgac ggaacattgg
     2221 tcaccaatcg ttcaaaagcg ttggaatcaa caaaacaagc aattgaatat gctcggaaaa
     2281 acggcatttt atgcggtgta gcaacggggc gcagcccagt aaagatcaaa gaaatcattg
     2341 atgaattgga attagatatg tatgtcgtgt ataatggtca actggttttt actgcagatc
     2401 gcacgattat cgaccaccct tttgaacaaa aagtattgga acatattgtt gaatttgcag
     2461 atgagaacca tcgacaaatc gtttttggtg cgagaaatcg gttagatgga tcaacgacga
     2521 tgctattagg acaatcgatt tttatcaagc gactagtcag ctttttaccg cgtaaattcc
     2581 cggtacgtct gatgaaaaaa atcctccagg tttttagccc tcaccgacaa aaggatcgtt
     2641 atgaaacgtt agagatatta aaagaaccaa tttatcaatg tatattgctc agtccggagt
     2701 cagaacagaa aaagttagaa catcgctttc ccgagtgtac gtttcagcgt tcaaatacgt
     2761 atactgtaga tatcatccca aaaggtggtt caaaattact aggcatccaa gctttcgcaa
     2821 atgctgtcga aattgaaatg gaagaaatta tggcattcgg cgatcattac aatgatatcg
     2881 agatgctgaa aggtgtagga attggagtgg ccatggggaa tgctcagatc gaagtaaaac
     2941 atgctgcaga ctttgtgact caatcaaatg aagaagatgg gatatacgaa ggactgaaac
     3001 acttcggtct gatttattag tcaatgaaag aaggaaaaaa tcagtatgga agcagagaca
     3061 ccttttcaaa aagtagaaac attccacaaa acttttgatc caagaagacc agagataccc
     3121 acggctttta cagaaaatga agctcataca cgtgctgcgt tcaaaataga agaacttgtt
     3181 gaatttgttg cagctacttc acctaataaa gaacaactgc atgaagctgt tatgctgctg
     3241 cacgaagcat tagataaagc agaagaaaaa atacttggta aaacacagtg gggaagtgat
     3301 ccgctggttg aacaagtaga tgcactgaca gatatccttt actttactta tggttctttt
     3361 tcattactag gaatagtgcc tgatcgaata ttcgaaatcg tccatcaggc aaatatgggg
     3421 aaattatttc cagatggaaa acctcattat gaccctgtaa caaataaagt gttgaaacct
     3481 gaaaactggc agatggaata tgctccagaa cctaaaatcg cggcagaaat ccaacgtcag
     3541 attcaagaag cgattgctaa gaagaaatcc gctgatatag gcatgtgcac aaatgaaaaa
     3601 gagtgatcag agtttcacta tttttgttat ctttatacga tggaaaaaca aaagattttc
     3661 gtttaagtta gtgaatgcat gaaaatagta tgctataatc tagaatagat gtcttttgca
     3721 cgagagacgt attatttgga tttatgccaa gcagaacgcc atctgttgca taaaagatgc
     3781 atactgaaaa cggaggaacc ataaatggga aataatgtta tcatcggaat cgtcattgca
     3841 atcattatag tcgccattgt cgtctttttg gttagttact tgatgaagaa aaaaaaccaa
     3901 gatcgattgg atgccttaga aaaacgcaag gaagaattat ttgacttgcc agtgattgaa
     3961 gaagtcgatg atgttaaaaa aatgcatctg gttgggcaaa gccaaaatac ctttcgtgaa
     4021 tggagtcaaa aatggaatga catatctact tcctcgttcg ctgaactaga aagtcaaatt
     4081 ttcgaagtgg aaaacctcaa tgaaagtttt cggttcttaa aaggaaaaaa agcagttgtt
     4141 gaagcagaag ctacaatgga ccaaatggag aaacaagtga cggaaatccg tgaaggtcta
     4201 aaggaattgc gggaaacgga agaacgcaac tcactagctg ttcagcaggc acttgacgtc
     4261 tatgaagaac tgaaagaaaa agtcagagaa gacgagcatg aatacggacc agctcttttt
     4321 gaattgcaaa aacagatcaa agtgatcgaa aaagaattta cgcagtttgt gactttaaac
     4381 acttctggag accctgttga agcccgcgag gttttggata aagcagaaaa acatacgtat
     4441 gaagtggaag ctctgatgaa aaaaattcca ccgctgtatg aggagctcca taccactttc
     4501 cctgaacaac ttgaagaaat cagtgatact tacgagaaat tgatggaaga acaatatgta
     4561 ttcccagaag aagatcttgc tgaagatatc gcaaaagtaa gtcgaagaat tgaaaattca
     4621 ttagccaatc ttgaaaaaac agaagttgaa acggtcgaat ttgaaaaccg tgaaacagct
     4681 gatttgattg attcgttata tgatatctta gaacgggaaa tggaagctca gagatatgta
     4741 aaaactaacc aatcaacgat tgcagagtat atcaaacata caacaaagaa taaccgtcaa
     4801 ttattgattg agctggatca cacatctcaa agctatacat tgaaccacaa tgaaattggc
     4861 cgtgtacgag gtttccaaac cgaagtggaa gaaatggaac gtcaaaatga acagatgatt
     4921 ccacagatcc gtcaacacga aattccttat tcagaagtgc gcacgttcta taaaacagtc
     4981 ttcaaagtct tagaagatat cgaaaagcaa caagtagaga tcgacgactc attacatgaa
     5041 ttgagaaaag gcgaaaaaga agcgcaagaa aagatcgata cattcgaatt caaattgcgc
     5101 agtttgaaac gttttgtaga aaaacaacgt ttgcctggct tgccgaatga ttacctagaa
     5161 ttcttttttg ttgctactga ccgtattgaa gaactaagtg ttgtcttgaa taaaattcga
     5221 gtaaacatgg aagaggtcaa tcgtttagtt gctctatgtg aagaagatct agaattatta
     5281 gataagaaga cacatgattt agtagatgca gcagcattaa cggagcaaat gctccaatat
     5341 gcaaatcgtt atcgtcatac acacgaagaa gtgagagaag caattgataa aagctactat
     5401 ttattcaata aagaatatca ctatcaagaa gcattagacg aaatcggtac agcattagag
     5461 cgagtagaac ctggtgcttt caaacgcatc gaagattttt attttaatca tccagactta
     5521 gtataaagca aaaaagttag taaaccagca agagcaagac ggattattgt cttgttcttg
     5581 tctttttttg tatatttgaa aattgaaaca agtgaaaatt tcacctgctc ttgaaagcac
     5641 cttggacaac cgttataata gaaaagaatt tcaaaaaaga ggggtttctt atgatatatt
     5701 ttgataacag tgcaacaacc ccaatctttc cgcaaagctt agatgcatat gtaaaaacca
     5761 gtcagcggat tattgggaat ccatcaagct tgcatgattt aggcaatcaa gctaaccggt
     5821 tgttgcagca agcaagaaag cagatcgctg atttgatcca tgtagaacca gaagaaatct
     5881 attttacttc aggcggaaca gaaggagata actgggtatt aaaaggaacg atgatcgaaa
     5941 aaagagaata tgggaatcat atgatcattt caggagtcga acatccggcc gtcagcgaga
     6001 cagcagaaca attaaaagaa ttaggaatcg aagtaagtat cgccccagtg gataaacgcg
     6061 gatttgtacg tgtcgacgaa ttgaaagaat taataagaaa agagacagta ttagtctctg
     6121 ttatggctgt gaataatgaa gtaggatcga ttcagcccat ccaggaaatc agtgatcttt
     6181 tggatgattt tcctaaaatt catttccatg tagatgctgt tcaggcgatt ggaaaagtac
     6241 cattctcgca atggttgaca gaaagagttg attttgcaac attttcagct cataaattcc
     6301 atggcccacg gggaactggt ttcatttatt ggaaaaaggg tcgtcgttta gcaccattac
     6361 tgacaggtgg agggcaagaa agaaatcagc gaagtgggac agaaaatgtt ccaggtattg
     6421 tggctatggt taaagcttta cgtttatcat tagagataaa ggaacaaaaa cctgagcaga
     6481 cacaacgctt aaaagtagct ctaatcaatg cgttgaagac gtatgagaaa gtgacgattt
     6541 tttcagaagg aactgactat gcaccgcata ttttatgttt tgctctaaaa ggaattcgcg
     6601 gcgaagttct cgtccatgcc ttagaagaaa aacaaatatt tgtttctact actagtgcct
     6661 gctcctcacg taaacatatg gcaagcagta cattgcacgc aatgcatgta ccaaatgatc
     6721 tggctacatc tgctgttcga atcagcttag atgaaaacaa ttcgcttgca gaagtcgagc
     6781 aatttatgat tattttcaag cagctttata agaaattttc tgcaatcaat taaaagagga
     6841 gtgttcttgt gaaatacagt gaaattatgg tccgttatgg ggaactttcg acaaaaggaa
     6901 agaatcgaaa aagctttatc atgcaactag cacaaaatgt cagacaagct ttgaaagact
     6961 ttccagaaat caaaatccat gctgatcggg accggatgca tcttttattg aatggcgcag
     7021 acgggcagct agttattcca aaactagcaa aaatatttgg gatccaaaat ttctctccaa
     7081 gtattcgtgt agaaaaagat gtcgaagttt tgaaacaagc tgttcaagac atcatgaaag
     7141 aaatttatac tgggaaagaa acatttaaaa ttatcgctaa aagaagcgat caccaatttg
     7201 aattgacaag taatgaattg aatcaaacat taggtaatgc tgtttttgat attttcccgc
     7261 atatccaagt tcaaatgcgt caaccagata ttccattacg tgtagagatt cggcgtgatg
     7321 gcgcttatct atcgtatgaa acgatcaagg gagctggagg attgcccgta ggaacaagcg
     7381 gaaaaggaat gcttatgctt tctgggggga ttgattctcc tgtagcaggg tactttgcca
     7441 tgaagcgggg agtggaaatt gaagctgttc attttgctag tccgccgtat acaagtgaac
     7501 aagcactgca aaaagcaaaa gatctaacag ccaaactagc accttatgtg ggaacgatcc
     7561 attttatcga agtgccattt accgagattc aagaagaaat caaaaagtca gtgccacaag
     7621 gatattggat gacaattact cgtcgaatga tgttgcgttt gaccgatgaa attcgccgta
     7681 ttcgtcatgg tttagtcatt ttgaatggtg aatcattagg tcaagtggct tcacaaactt
     7741 tgcaaagtat ggtggctatc aatgaagtaa cgaatacgcc aatcattcgg cctgtagcga
     7801 caatggataa acttgaaatc attgaagtag cagaacagat cgatacattt aatctttcta
     7861 tccagccttt tgaagattgc tgtacgattt ttgctcctcc acagccaaaa acacgccctc
     7921 aactggaaaa ggtccatcaa tatgaagagc gtttagcaat agatgaaatg atccaacgag
     7981 cactagctgg cttgaagatg gaagagatac aagcagaatc gtctaatgaa aaaactgaag
     8041 aattttcaga attgctataa gaggttatga aaatagtgtt ttgaccagag tattagagaa
     8101 acaaaacgaa aaaatagctc catgtatttt accgtttttg aactaatgtc gaagattagc
     8161 tgattgaata ccatttatca agaagttatg gaaaagccgg ttctttttta ctaactgaaa
     8221 aataaaattt tatttagaga tcaagctaca gttgataaat gatataaaaa agagaatttc
     8281 ccatgaaata g
//
LOCUS       DACEMO010000118         8034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEMO010000118 DACEMO010000000
VERSION     DACEMO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.121"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IW728_001956"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IW728_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1025306.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="IW728_001957"
     CDS             1228..2331
                     /locus_tag="IW728_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1025307.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IW728_001958"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IW728_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1025308.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3821..6268)
                     /locus_tag="IW728_001959"
     CDS             complement(3821..6268)
                     /locus_tag="IW728_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1025309.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6457..7893)
                     /locus_tag="IW728_001960"
     CDS             complement(6457..7893)
                     /locus_tag="IW728_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1025310.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2235 a         1739 c         1373 g         2687 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aagatggaaa aaaaccattt tttcttttgg tatcatcatg ccttgtcttt
     3781 ttattggtta ttcgtttttc aacccttcat cacacaagtt ttacatgtca ttatcgtccg
     3841 gcaacgtttc tgatgtttct tcctgctcaa tgaatacttt ccgaggcttg cttccttctg
     3901 acgggccaac aactccatga gcttccagtt catctactaa acgtgctgct cggttgtaac
     3961 cgatccgaaa tcttctttgg agaagggaaa tacttgccgt ttgcatttca acgacaagtg
     4021 cttttgcttc ttcaaacagc tcatcttgtg gctgttctgg agcacctgtc gtttcaactt
     4081 catctgtcgg catcattttc tcttcataat gagcttcttg ctgatctgta acaaattgaa
     4141 cgacccgttc tacttcgtgg tcagaaatga atgctccttg cacacggatc ggtttattct
     4201 cacccattgg caagaatagc atatctcctc gccctaagag cttttctgcc ccattagaat
     4261 caatgatcgt ccgagaatct gtaccgcttg ataccgcaaa tgccattctg gaaggtacat
     4321 ttgctttgat gattcctgtg ataacatcca cacttggacg ttgagtagct aggatcatat
     4381 gaatccccgc tgcccgagcc atttgcgcta aacgaataat cgcatcttca acttcattgc
     4441 tggctaccat catcaaatca gctaattcat ctacgatcac tacgatgaat ggtaaggttg
     4501 gatgtttcgt tccgttttcc aagtttttct gttgaacaaa ttcattgtaa ccagaaatat
     4561 ttcttactcc ggttgccgcg aatttttcat atcgttcttc catttcttgt actacttttt
     4621 gtaaggcttg cgctgctttt ctagggttcg tcacgaccgg cgtcaacaga tgaggtatgc
     4681 cgttgtacat attcaattca accattttcg gatcgatcat catcagtttt acttcatggg
     4741 gcttagcacg cattaatata cttgtgataa tcccattaat tgctactgac ttcccactac
     4801 cagtcgaacc agccacgagt aagtgaggca tcttcgaaag atcagccgtt tgtacccgtc
     4861 cagaaacatc acgtcctaaa ggtacttcta gtaatttatc tgggtggttt ggttgtgctt
     4921 cgatgatttc tctaaaggaa accatactga tctttccatt tggtacttca ataccaatca
     4981 gtgatttacc tggaatcggt gcttccatcc ggacatcttt cgccgctaaa gcaagcgcga
     5041 tatcatctgt taagttcaca attttactta cttttacccc tacagccggc tggacttcga
     5101 attttgtgac ggatggtcct aagctggctt tgacgacctt ggcatcgaca ccgaaacttt
     5161 ggaaagtttg ttctaatacg ccgatatttt tttcaatttt tttatactca tcactttgat
     5221 ccacagttgg tatagaatca agcaaatcaa ctgtcggtaa ttcatagtct tgattttcct
     5281 gttctgcttc aatctcaaat tctaatgagg taccgtcatc ctctggcaac tcatctgaaa
     5341 aaccttctgc tgtattggtc tgttttggtg tatctggtac tggtttctct agattcgcag
     5401 gatggatatt ttcttgaaaa ctgtcgattg gaacaaaact tagctgctcc ggctctgtat
     5461 tttctggttc ctgccattca tcagcagtat tttccctttg ctggctcacc gcttttttct
     5521 tctcatattc tgcagcttct ttttctgcag cttcttttgc agctaagcgc cttgcttctt
     5581 tcgcttctgc gcgctgtttc attctctctt cctttgccgc ttgctttcga gcttgcttgg
     5641 caggatcacc ttccaataat tcctgtactc gttctccaat tgattggata ccatttaaca
     5701 gctgatagcc ttccagatta ctgaacagga atgctcctcc tgccagtaat agcactgctg
     5761 ctaaataact tccaggctgt gcaatcaaga agtaagtaaa atgataaagg attgctccaa
     5821 tcatcccacc accaacgtta ttgtctactt gactttgttt tatatcactg gctaacaaat
     5881 cccatgtcgt ccccatgata tttggttccc cagtatggag tttgccaaat aaatgggcat
     5941 gtaacaataa taagataccg aaataaaaga gtatgccgcc aaaccagcgt cggtttttac
     6001 caattgaaaa ctctgtattc ttaatgacaa tccaaaatcc taatacggct aataaaagac
     6061 agaggatttg gtaagtattt cctgcaatga tccgaaagcc gtttgcaatc aatattccta
     6121 aaaaacctaa ttttaaaaag ccgaagacag caaataaaat caaggcaaac ccaacaccaa
     6181 tcgtcagcat ttgttcctgc tgctgttttt gctttttcgt ttttttcgtc tttttccttt
     6241 ttttagtagg ccgtttcttt tgtgccattt attatccctc atttcatcaa gtttcattat
     6301 acaaaaaaaa gtaagggaac taaagttttg tttgaaagaa ctgacgcttt ctttcatttt
     6361 tcagcttatt ttacagtttt tcttaatttc taaatgattt tatcaagaaa aggcacggcc
     6421 atatccagcc gtaccttctt gttttaagta tgtgaactaa aggttctctc cattttcttc
     6481 aatcagtttt tggtaccagt aataactgtc tttcttatat cgttccaaag atccttcttg
     6541 tgtttcgtct tgatcaacat atacaaatcc atatcgcttt tgataaccgt tcagccagct
     6601 aagcaaatct gtataagacc aggtgcaata gcctaggact tctgctccat ccgaaattgc
     6661 ttctttgatg gctttgacgt ggctttgtaa gtaattgatt cgatagtcgt catggatttg
     6721 tttatcttct gtcaacttgt catactctcc taagccattt tccgtaatca gtaccggcat
     6781 gcggtaacgg cttgtgatac gacgcagtcc gatccttaat ccttctgggt caatctccca
     6841 gtcccaattt gttcgttcga caaatggatt ctcaactttt ttatacattc caggaactcc
     6901 agtttcttct gatgaacctt tttttcctgt cgtattcatt tttccagcac caactccgtt
     6961 taatggattg aatgcattcg tcgccgtttg atagtaattg atcccaagaa aatctggttt
     7021 agcagattct agcaattctg catcgccagc ttcaatcgtt ggtgcccatc ctttttcttc
     7081 taagaacctc atggcagcaa tcggatattt tccccacata tagacatcca tccaataatt
     7141 ggccatcagg tcttctgcat tttcagcagc cagaacattt tcaggattac tgtcaattgg
     7201 ataatttggc gtatacgcaa agcttggtcc gatttttcca ggcatttcta actcgtgaaa
     7261 tttattgatg acagatgcat ttgctaaatt agcaatatgg ttaacttcat acattcgttt
     7321 cgggtctgtc acacctggcg gatgtgctgc taacaaataa cctaatgaag tgaagatatt
     7381 ttgttcattc aaactaatcc agtaatggac tttcccgcgg aatgcctcaa ataatgtttc
     7441 tgcataatga gtgaaatctt caatgatctg acgtgattcc caaccattat attcatcttg
     7501 caatgcttgt ggaagatccc aatgatataa agtcaacata ggttcaatat cgttttcaat
     7561 caattcgtcg atcaatctct cataaaaagc gagcccagct tgattgactt ctcctcgtcc
     7621 attaggaaaa atccgtgtcc aagctacaga gaagcggtaa gctttcagac cttgttcttt
     7681 cattaaagca atatcttctt tataacgatg atagtggtct actgccaaat caccgttcgt
     7741 tcctttaaat gtctttccag gtatccgtac aaatcgatcc cagactgatt ctcctttccc
     7801 atcttcttgc caagctcctt ctacttgata cgctgcagaa gcagagcccc acagaaaatc
     7861 ttttggaaat gaggtaagtt ctttgtgttc cattcatctc ttcctttcct ataaccttca
     7921 atcgttcctc ttttagaata acacgaaacc ccttccacaa caaaataaaa atatgtaaag
     7981 gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa aattttgtga aaat
//
LOCUS       DACEMO010000119         8018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEMO010000119 DACEMO010000000
VERSION     DACEMO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.122"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            390..2483
                     /locus_tag="IW728_001961"
     CDS             390..2483
                     /locus_tag="IW728_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1025311.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            2643..3509
                     /locus_tag="IW728_001962"
     CDS             2643..3509
                     /locus_tag="IW728_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1025312.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(3531..3968)
                     /gene="ndk"
                     /locus_tag="IW728_001963"
     CDS             complement(3531..3968)
                     /gene="ndk"
                     /locus_tag="IW728_001963"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1025313.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            4627..7305
                     /gene="mgtA"
                     /locus_tag="IW728_001964"
     CDS             4627..7305
                     /gene="mgtA"
                     /locus_tag="IW728_001964"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1025314.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            7392..>8018
                     /locus_tag="IW728_001965"
     CDS             7392..>8018
                     /locus_tag="IW728_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1025315.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKD"
BASE COUNT         2622 a         1367 c         1691 g         2338 t
ORIGIN      
        1 caatttcaaa aaacagcttc tcatgtttta taaatattgt gacttgtggc cgaggagttg
       61 gcaggagaac accgtttaag gaagaagttg tgaaaaagct gtcctgcatc aagaaataag
      121 gagctgcgac aagaattttg tcacagcctc tttttttgta cgaataccga gaaactggct
      181 tttgaatacc gtggagtgaa gcggtcacca caagactttt atctcttcat tagtttaatc
      241 gcttacatcg cagcggtttt atgctgtgaa aagtggacgg aataatcttt agaaagaata
      301 aaaaaatctt aagataacaa cttttttagg aaattttcat ttttcaggag caaaataaga
      361 aaaaatgtga atagagagaa gtgatatacg tgacacaact taaaaaattg tgccaaaatc
      421 gtttagcaat cgttttgaca gctgttttct ttttctggct gaagacgatc attgcctact
      481 atgcagattt ttcattaggt gtggaaggta cgatccagta ctttatcttg tggatcaacc
      541 caattgcgac taccttattg ttttttggcc tttctttgta tattaaaaag ccaaagccag
      601 cgttagctat tcttttgatc attgatattt taaatacgct tttgttgtac ttgaatatta
      661 ttttttatcg ggaattcaca gatttcatta cagtcaaatc tgtattaggc ttttcgaaag
      721 tgtcgcaagg attgtcagga agctcctttt ctttgatgaa gccccatgat gtcatctact
      781 ggctggatat cgctgtgttc attgggctac tggtttggtt gaaggtcaag aaaataccta
      841 tcaaaagcaa ccctgtcggc aaaccgatgg ctatcgctgt tacctgtctt tcagggctta
      901 ttttttcagg aaatctagcg ctgagcgaag ccaaccgtcc acaattgctg cagcgaacat
      961 ttgaccgttc atatattgtc aaatatctag gaatcgatgc gtatactatt tatgacggaa
     1021 tcaaaacagg aatgactagc agtgttcgcg cgcatgcaag cagtaatggg attgatgaag
     1081 ttttagatta tacaaagaaa cattatgcag agccaaatcc cgaaacattt gggatcgcta
     1141 aagggaaaaa tgtgatcgtt ctccatttag aaagtttcca gcagtttttg attaatatga
     1201 aggtagacgg acaagaagtc acaccgtttt taaatagtat tttccagaat caagcaacga
     1261 tcagctttga caatttcttt catgaagtag gacagggaaa gacaagtgat gctgaaaata
     1321 tgttggaaac tggcacattt ggtttacctc aaggctcatt attcacagaa cttgggtcag
     1381 acaatgtctt tcaagccgca ccagcgatcc ttggacaaaa gcaaggctat acaagcgctg
     1441 ttttccatgg gaatgtagct agtttttgga atagagatca tgtatataaa aacttaggtt
     1501 atgacaattt ctttgatcga tcttattttg atgaatccga cgaaacgtta ggttatggga
     1561 tattagataa agatttattc agagaatctg cccaatatct tgagcatcta caacagccgt
     1621 tttacacgaa atttctttcc gtaaccaacc acacgcctta ttatacggat gacaagaatt
     1681 tcgactttcc atctttgaat actggcaata gcacagtaga taattatgtt cgaacggcac
     1741 attatctgga tcaatcattg gaacaatttt ttacgtattt gaaaaaatct ggtatttacc
     1801 aaaattcaat ctttgtgatt tacggggatc attttgggat ctcgaataca gataataaag
     1861 atttagcaag cgctcttggt aaagatccag atacatggga tgaatttgac aacgcacaga
     1921 tgcaacgagt gccattgatg atccatatgc ctggttatac taaagggaca gtcaatcatg
     1981 aatacggtgg agaaatcgat gttttgccga cattactaca tctattggga atcgatgata
     2041 aagattatct tcattttggt acagacttat tatcttctca gcatgatcaa gtagttgctt
     2101 ttcgaaatgg gaactttgtg acacccaaat acaccgtcct aggaggaaaa gcttacaata
     2161 atcagacagg agaaatcatc gatacgaaag cagcaggaat cgatgaggaa atctccaaag
     2221 atcaggaaaa agtcaagact gcgttaagtc tttcagataa gctgaatcag gaaaatctct
     2281 tgcggtttta tgtgccaaac ggatttgaga cgattgatcc gaaaaaatat gactataaaa
     2341 atgaagcgga acgtaatgaa gcaatcgaaa agcaaaaagg cgaacattcg acaagtgtct
     2401 tttcaaaaaa tggtaataaa acaacaacca acttatatcc agtaacaaga acagacgttg
     2461 aacagcaaaa aaataatcaa taaacaatca aaccaacctt cagtagttag acaacattct
     2521 aatgcttgaa ggttggtatt ttttatgaaa aaaacatttt tttatttttt tcgtgtattt
     2581 ttccttttta acgtatctat ttattgaatg tggaaaaatt taaattaaaa aggagaacta
     2641 ttatgtatga tggtgaattg atcaagaaac ttcgagtcaa caagaagttg acacagtccc
     2701 aattagcgga aggaatctgt tttaagactt ctctagtcgg gattgagaca aattcagtga
     2761 aaaaaatgtc atttgtaaca ttgagatcat ttttagagcg aatgaacatg acacttgcag
     2821 aatatgaatg gatgcgaaac cagctggatg aaccaataaa gttaaagaaa aaaagacgaa
     2881 tgctagatcg ggtacaagaa gacagctttg atccttataa agagatcagc aataaccgta
     2941 ggcattttaa gaaaacagaa gatctgtatt atttggtttt gaatcttcac atggtcgaaa
     3001 aaacagaagt atctgagaca gaatttgaat ttttacgtta tgaatgccga atcattgaag
     3061 aatacttgat gcagatacca gactatggtc aatttgaact atctattcta gctgattttc
     3121 cttggatatt cagtgatcgg ttcattgaga acaattattt gaaaatcaaa aaaagaatgc
     3181 gacgattagc acagttgaac aatcatgagc agtatctctt tactttttta atgaacctga
     3241 cttcgtttta tatagaaaat ggtcggttga aaaaggcacg tagtgtgaat gaagatatga
     3301 aacggagctt atcacataaa gaaagaagta cagtgatcta tgaaacattg atggcagatt
     3361 attatcaaag attgatttct gctgctttag gggaagaaaa gacgaaagac taccaagaat
     3421 tcttcacagt gctcgaatat atgttaggaa agaatcaacg gaggtcctta gagaaaaaac
     3481 tgctggccat tgctgaaccc agcaactaga ccagcagttt agaatttgta tcacttcgcc
     3541 gctttttcag acggtttgtt tatatcgaat ggcgggaaaa agcgggcgat ctcttttacc
     3601 gcagcatcac gggaatcaga agcgtgaatg atgttttcag tccccggcag cgcgtagtct
     3661 cctcggatcg tacctggaac agcatcggca gcttttgttg ctccaaccat cttacggacg
     3721 atatcgatca cttcctcgcc gaccagcaca agataaacga caggaccgga agtcatgtaa
     3781 tcgatcagtt catcaaaaaa actgcgctca gtcaagtgct gataatgttc ttttgctaat
     3841 tcaggagtca tagtttcaaa cttcatttca gcaatggcca gtccctttgc ttcaaaacgt
     3901 tggatgatgc ttccgactaa atggcgtctc acgccgtctg gcttgataat aacaagtgtt
     3961 ctttccataa cagcacctcg tttcctacta tatttctatt atagcgggag gtggaaaatg
     4021 agacaaataa aaccgcgtga gacaggaata taacgtgtgt attttaaaaa aagtatgaaa
     4081 aacatggaaa attgatttga catcctcttt tttagttgtt atattaacga aggaaaaccg
     4141 aataagattt gttaggtgag gctcctatac aaacataggc tactgcccaa aaacgtcgag
     4201 agacgccaat gggtagaaca ggaattgtcg agataaggct tttcataagg tagctaaaag
     4261 caggtatctg ctttttacgt tgtacagtgt caaaactcaa cgatgagatc tgagaagctt
     4321 tttttaatat gctttttcaa acctttcgct gagtatttgg cgagaggttt ttttgttgcc
     4381 gcaaatcccc atcggtgaaa aataaaaagg aggtggtcta attggaaaaa taactattcg
     4441 aaaaatgatt ggaagtcatg aattaaaatt acttagtaaa ggagatgaga acaaatggat
     4501 gacagtcatt cgaaaaacaa ggatctagag gaaaggccag ggctattatc tatctgttct
     4561 cagccccttt cagttttttc tctatcgatc cgatgaatag ctaaagacca aaagcagcga
     4621 gaacagctgt cttttggaca caaaggagag attgagatgg aaaaaaagaa agatacacaa
     4681 aaaatctatg cagcttttgc tggaaaaacg aaacaagaat tatttgaact attccatgca
     4741 tcagcaaatg gattgacaga agaacaaatc gaacgttcaa gagaaaatta cggtgaaaat
     4801 gcgatttcat atggcaaaaa aacaccattc attgtagaaa tattgaaagc atacatcacg
     4861 ccatttacat tagtgttgat cgcattagga attatttcat tcatcacgga atatgtatta
     4921 gcagctcctg gagataaaga tcttttcggt gtagccatta tcttcactat ggttatcgtc
     4981 agcgggacaa tgacgttgat tcagtccgta cgttccaatc aagcggctga aaaactgaaa
     5041 tcacttgtga aagtaacggc agctgtaaaa cgtaatggaa agtacacaga gattcctatg
     5101 gaagaaatcg tctgtggaga tcttgttcgg ctttcagcag gggatatgat accagcggat
     5161 atgcgtttat tgtccgcaaa ggatttattc atttcccaag cagcattgac aggggaaagc
     5221 tatccagtag aaaagcatgc gaaagtatgt gaagacgatc agcaaagcga aacaagttac
     5281 gaaaatcttg tctttatggg aagtaatgtc atcagtggaa gtgcggaagg aatcattgtt
     5341 tctgttggaa atgaaacact atttggtcat gtagcagaaa cgctatctga aaaaccaatt
     5401 caatcaagct ttgaattagg catccataaa acatccatgc tactgatcaa atttatgtca
     5461 ttgatggctc cagcagttat cgtcatcaat gggcttacaa aaggggattg gctagaagct
     5521 tttctttttg gactatctgt agcagttggt ctaacacctg aaatgcttcc aatgatcgtt
     5581 acgacgaatt tggtcaaagg agcaagtgtg atggccaaaa aaggaacagt gatcaaaaat
     5641 ttgaatgcca tccagaactt tggtgcaatc gatgtgttat gtacggataa aacggggaca
     5701 ttgacacaag ataaaatcat tcttgaatat catatggatt gtgaaggaaa tgaagatgat
     5761 cgtgttttgc gccatgcgta tctaaatagc tactatcaaa cagggctgaa aaatttgtta
     5821 gatgtggcta tcattgatga agcaacagaa actttagata cagataagat caattatcaa
     5881 aaagtggatg aaattccttt tgattttgaa cgtcgtcgta tgagtgttgt ggttgctgat
     5941 ccagctggta aaacacaaat gatcacaaaa ggcgcgattg aagaaatgct gaacatttct
     6001 aaatttattg atcttggcgg tactgttaca ccattaacaa aagaacgaaa agaagccgta
     6061 cttgcaaaag tacagtcact taatgaagat ggcttacgag tgattggtgt cgcacaaaag
     6121 accaatccaa gcgtcgttgg agaattttca gtcaaagatg agtcagacat ggttttgatt
     6181 ggttatcttg ctttccttga tccaccaaaa gaaacaacga aacaagcgtt gaaagctctg
     6241 aaagaacatg gtgtagctgt aaaagttctg actggggaca acgaattagt tacacgttca
     6301 gtgtgccgtc aagtaggtct agagatcaat gaattggtta ccggtgagaa aatctcggaa
     6361 atgagtgatc atgaattagc acaagtagct gaagaccatg aagtattcgt aaaattgaat
     6421 cctcagcaaa aagcacgttt gacgacagct ttacgtaaaa atggtcatac tgtcggattt
     6481 ttaggagatg gtatcaatga cgctcctgca atgaaagtgg ctgatatcgg tatttctgta
     6541 gatacagctg tggatattgc gaaagaatct gcagacgtga tccttttaga aaaagatttg
     6601 acgattttgg aaagaggcct tctttctgga cgagaaactt ttgggaatat catgaaatat
     6661 atcaaggcta cagcaagttc taattttggc aatatgtttt ccgtgctgat tgcaagtacg
     6721 ttcctgccat ttttaccaat gctgccttta caacttttgt ttttgaatct gatctatgat
     6781 gtttcctgca tttccttgcc atgggatcgg atggataaag aatatttgga cgagccaaaa
     6841 aaatggaatg catctagtat tggggaattc atggtttact ttggtccaac aagttcgata
     6901 tttgatatta ctacttactt gttgatgtat tttgttattt gtcctgctgt agtgggcgga
     6961 agtttccata cattggatag ttcacagcga gtgattttca tcgcattatt ccatgcagga
     7021 tggtttgtcg aatcactatg gacacaaacc ttagtgctcc atgcactgcg tacaccgaaa
     7081 gtaccattta tccaaagcaa cgcaacattt gccatgtttg ttatcactac attaggaatt
     7141 attgtcggtt ctgtccttcc ttttacaagt tttggtgctg aattaggctt gatgccactt
     7201 cctggcaatt actggttatg gttatttgct acagtcatcg cttatctggc gcttgtgaca
     7261 gctgtgaaga aaatctatat ccgtcgattc caagaactct tataaacgag aaggctggtt
     7321 ctttgcttct gttctttttg gaccagtcgt gctaaactat gtgtattgtt ttggaaagga
     7381 ttaatgtata gatgaaaggt gcaatatttg atttagatgg tttgttagtt gacacagaaa
     7441 aaacgtatcg tgatggctgg ctttggggat ttcagcaatt tgatatccat atcccaaaag
     7501 aaatcgttga cgcttggggc ggaaaaaact ggaaacaaag ttttgatatt ttagtgaaag
     7561 cagcaggtag ccctgaaaaa gtacaggaag tccgagcgaa gcgtgaagaa tatttttatc
     7621 aacagctgac aagcggagcc atccacttaa aaccatatgc aaaagaaata ttggcagact
     7681 taaaagaacg tcatatgaaa ctaggcattg ctacgaccac tgttacgaaa agagcaacag
     7741 atatcttgga tcactttaaa ttagctgatt acttggacac gtttacattc ggagacgaag
     7801 tgtcggagaa caagccttca ccgattcctt atctgacagc tttggaacga acaggattag
     7861 aagctagttc agcctttgca gtggaagatt ctttagtagg agcaacagct gcttcacgag
     7921 caggattagg cgttgttttg atacctgatt ccagttttgt ccgaaattat accgtcgaag
     7981 aaaaggaaaa attgaaactt cttgcggaag gaaaagat
//
LOCUS       DACEMO010000120         8009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEMO010000120 DACEMO010000000
VERSION     DACEMO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.123"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(222..719)
                     /locus_tag="IW728_001966"
     CDS             complement(222..719)
                     /locus_tag="IW728_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1025316.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(759..1568)
                     /locus_tag="IW728_001967"
     CDS             complement(759..1568)
                     /locus_tag="IW728_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1025317.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1902..2759
                     /locus_tag="IW728_001968"
     CDS             1902..2759
                     /locus_tag="IW728_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1025318.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2752..3654
                     /locus_tag="IW728_001969"
     CDS             2752..3654
                     /locus_tag="IW728_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1025319.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3687..4037
                     /locus_tag="IW728_001970"
     CDS             3687..4037
                     /locus_tag="IW728_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1025320.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4071..5009
                     /locus_tag="IW728_001971"
     CDS             4071..5009
                     /locus_tag="IW728_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1025321.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5087..5980
                     /locus_tag="IW728_001972"
     CDS             5087..5980
                     /locus_tag="IW728_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1025322.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6018..6581
                     /gene="amaP"
                     /locus_tag="IW728_001973"
     CDS             6018..6581
                     /gene="amaP"
                     /locus_tag="IW728_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1025323.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6595..6783
                     /locus_tag="IW728_001974"
     CDS             6595..6783
                     /locus_tag="IW728_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1025324.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6796..7698
                     /locus_tag="IW728_001975"
     CDS             6796..7698
                     /locus_tag="IW728_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1025325.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7730..7972
                     /locus_tag="IW728_001976"
     CDS             7730..7972
                     /locus_tag="IW728_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1025326.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         2782 a         1401 c         1563 g         2263 t
ORIGIN      
        1 ttaacaaacg gaaaaatagt tcctcatatt ttgccgtttg ttaagctaat accgagggtc
       61 agctgattga acaccattta agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata
      121 aaaaagccaa tctactttga tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt
      181 ttttattgtg tgttaaatat cgtaaatgaa gatagatatt tttatgattc gacgatagag
      241 acgttgaaat ctttcaacag ctctaaagcg gattctccgt gttttctact ggagactaac
      301 attggatcaa caaaaaagtc tgcttcagga aaagcactct gcaggcctat cagattagag
      361 agtacacaca agtttgtttc aatcccgact acttcgatct ctttttgttc ttctttgctt
      421 tcaaataaag tttcctggat ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt
      481 ttaatcactc gttcattttc ttgtggagaa agaagaggga tcaattgcaa atcttctcgt
      541 tcttcttctt tgtccttcag ttcaactggt atatcccgtg tgaagagaat atattcatta
      601 ttttggcgtg ctttagctaa tctttgagca atacgttctg ccaattgatc tgactctgga
      661 atataaaatt cgctgtttgg atcaagaata tgattttgca tatcaataat aactaacatt
      721 ttctttcctc ctacattcat atcgtcattt tattcttttt aatagaagtt ttttaaaata
      781 taagcaacac cagcttcttc attcgtcagt gttacttggt ttgcaatttc ttttacttgt
      841 cgctgtgcat tccccatcgc aatactcaac ccgctgtaat tcaacatatc aatgtcgttt
      901 tcttgatcgc caaaagccat gatgtcactc gattcgattc caaggtaatc cgccagaaaa
      961 acaagtgaat gagctttagt gatacctgta ggcgtgattt ctaagaaatg cggatcagag
     1021 aatactgatt ggataccatc tgcttgcaga tctctttgta gcttctgtag tttttcttca
     1081 ctcttatctg ttaatgctaa tttaagatag ctaagagaag gatcttccaa catatttaaa
     1141 atttgatcaa aagaatggtt ctgtaaattg aaatgttcat aataagggtc atctttgact
     1201 agatttgatt catcgaatgt cagctgatca tcttgggttt ctcggctagc aatgatggat
     1261 attttcgctt cttgagcata gaagaaggct tttctcagtg tatcgctatc tatttttgaa
     1321 cgtctgagaa ctttcgccat ttttccatcc ttcatttcag caatcagtga accattggaa
     1381 gaaatcaaat agccaccgat tcctaattcg gaaaaaatat ctttagcaga aatgctgttc
     1441 cttccggtag cgacggttac gattccgcca ttttcatcaa atttccgaag tgcttggata
     1501 ttttcttctg gtatctttcc atttcgatca agcaaggtac cgtctaaatc aatcgctgct
     1561 agtttcattt tatcttccta ctttcttttt acatatctat tttcctgtta ccttaagatt
     1621 aaatgagaat ttaaaaaaat gaaactaact tgctttgtca ttatgaattt tttcaaaaaa
     1681 cattcttaag aaaaaggtgg ctatatatag tagtttttgg tcaatacctt tgaataaaaa
     1741 gccatgaaaa acgaatagtt agcgcttacc gatgcgtttt gtgataaaaa aattttaaaa
     1801 tattcaatga aaacaaagca tatattttgc tttaaaacca aatgaggaat attctagatg
     1861 tagaacaggt tagaaaaaga ttagaaaaag gagtgatttt gatggagaag agatacggcg
     1921 tagttcctgt ggtaactgct tcagacgaaa attatgctcc ttacttgagc gtgatgatcg
     1981 caactgcatt agaaaattgc aataaaacaa gacgcataaa attttatgtg attgacgatg
     2041 gtctatcaga atatagtaaa gaaggattgg aagaaacggt aaataaatat tcaagtaatg
     2101 caagtatcca attcctgaca gtggaaaaag atatctacga agacttccta gtcagtgacc
     2161 atatcacaac taccgcttac ttaagaattt ctttaccaaa cttattagct aaagaggatt
     2221 ataagaaagt cctttactta gattcagatg tgttagtact agacgatatc gtaaaactat
     2281 atgatgagcc gttaaatgga aaaacaatcg gcgctattat cgatccaggc caagtgaaag
     2341 ctttggaaag attaggaatc gattccgatg acttgtactt caattcagga gtgatggtga
     2401 ttgacattga tcaatggaat aaaaaagaaa ttacagaaaa aactatccat tacctcagtg
     2461 aaaatggcga tcgaatcatc tatcacgacc aagatgcttt gaatgctgta ctttatgaag
     2521 attgggaaca acttcatcca aaatggaaca tgcagacttc gctgattttt gaacgtcatc
     2581 cagcaccaaa tgaaaaatat gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg
     2641 ttcactttac aggacatgat aaaccttgga atacgttgaa agatcatccg tataccaatt
     2701 tatacttaaa aaaattagca cacagtactc taacgaaagt aggcgagatc aatgaataaa
     2761 aaagaaatag cagtagtcgc aagttgtaac acaaaattcg taccacattt agctgcatta
     2821 tttgtctcag tattagataa ttgcaacccg tctaaatttg ttcggttcta tgtgattgac
     2881 gatgatatcg attttgaaag taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg
     2941 aactcagatg tggaattctt gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc
     3001 gatcgtattc cagaaacagc ttattatcgt attgcgattc cggaattgtt ccgcggcaca
     3061 gaagttgaac gtatcttata tatggattgc gacatgatcg ctttacaaga tatctctaaa
     3121 ttatggcgtc ttgacttcgg tgattcaatc gtagcagctg ttgaagacgc tgggttccat
     3181 caacgactgg aaaaaatgaa aatttcagct aaatcaatgc gttatttcaa ctcaggtttg
     3241 atgctgatca atgtgaaaaa atggttagat gaaaatatta cgcaaaaagt attagatttc
     3301 atcgaacata atccagaaaa attacgattc catgaccaag atgcattaaa tgctatctta
     3361 catgaccgtt ggttaccact tcatccgcgt tggaatgcac aaggctatat catggcaaaa
     3421 gcaaaaaaac atccaacagc agcaggtgaa cgtgaatacg aagaaactcg caacaatcct
     3481 tacattatcc atttttcagg acatgtgaaa ccgtggagca aggattttga agggccaacg
     3541 aagaaatatt acgaaaaata tgcagggatg acggctttcc gttgtgtagc taaattccct
     3601 aaatatccaa aatatgcaaa agttcaacca aaaacagatg tagttgttaa ataatagaag
     3661 acaacaactg aagaggggga gtaggaatgg aaattacaat gtctaaaaat gcagtagaga
     3721 cgttaattga aaaggttggg gaaaatacaa aaatcgcact cgcgcttatc aatgattccg
     3781 atccatttct aagggacaaa ggtgcttttg caaaaggcag cttttttcaa atcattcctt
     3841 ttgtttcgga atttggagaa tatgcgacta aaatcgagca tccattattg gacatctata
     3901 catctaaatt agagcaaaat tatttcggta aacgattgaa catggacttc aacaaacagc
     3961 ttgacagctt ttcattagaa aatgaaatcg cagtactcga ttataatatc aaattaaaga
     4021 actgcttctt cagctaatag tttcgagaaa gtccaaaaag gaggtataca ttgagatact
     4081 actttgtgga tgaagaaaag aatgtaatag aaacagatga aaaaaaatat aattggctcg
     4141 tacttgatag tgcaaagccc gaagagattg agcaagcaat cgaagaattc gagcttcctg
     4201 atgatatttt cgtaggaacc gaatatccgg aagaagtttc tcggatggag cgcctttatg
     4261 agacgaaact agagcatcca ttttctttgg tagtgattaa tctagatcag tcagataatc
     4321 gtatcgaaaa aaaattaacg ccaatatctt ttgtactttc cgataatctc ttgattactt
     4381 gtatcgatag aaaaaccgaa tttgtggatc ggctgctgac aaagcatgga aatcatttaa
     4441 actcgtttga aaagatcatc acgtatacat tattagatat ttacacccat tacgtgaaag
     4501 aactaagaga gatgaaaaag cgaattgacg ctttggataa agcagctcgt aaaacaacgg
     4561 aaaatgaaga actgtataaa caggcagatc ttgaaagaga catcgtatat atcgatcata
     4621 ccttaagaga tcaacgggaa acaatggatc aattatggaa ttctgaaact ttcatcaaac
     4681 gtttagacga tgaacagcta ctctatgatg tccatttgag acagcgacaa acagaaaaaa
     4741 tgatcgagat ctatcgagat ttattagaga gtattggtga tctattcaac gggatgatgg
     4801 acaataatct aaatcatatc atgaaatatc tagattcagc tgcattaatc atttccgttc
     4861 cggcgttgtt cgcagggatt tggggaatga atacaggagg attaccgggt aaaagttctg
     4921 ctctaggctt tatactcgta gtctttggct ctttgatagc agcagtagtt ttaggttacc
     4981 atttataccg gaaagattat acaaattaaa gaatctattt aaaacaggta tcaaacaaag
     5041 aaatttgttt tggcaataca atttaaaaac taggaggaat acagcaatga cagaaccaaa
     5101 attaatcgac ccgcgaaact tgtaccatac ggagaaattt cctaaacaag atcaggatac
     5161 accagcttta cagcagaata tgattcctgt tcctgattgt ggagaagaat cctatgaagg
     5221 caataatcaa ctagaaaacc gccgtgtact aatcactggt ggagattcag ggatcggtcg
     5281 tgcagcagcg attgcatttg ctagagaagg ggcagatatc gctcttcaat tcttcccagg
     5341 agaagaaaaa gatgcagagg aagtagcaaa atacgtgaaa gttgcaggac gtaagattgt
     5401 cttactacct tttgatcttc gtgacgaaca agcacctaaa gaaatcatag ataaagccgt
     5461 gagtgaactt ggcgggctag atacgatggt actaaacgca ggacagcaaa tttcttgtca
     5521 gtctattgct gagttaccac ttgagcaagt caaagacaca tttatgataa acatcatcgc
     5581 tatgtttgct ttagtgaaag aagctgttcc gcatatccca gcaggaggag caatcgtaac
     5641 aacaacttct gttcaagcat tcaatccaag cgaacattta ttagattacg cagcaacaaa
     5701 agcttcaatc gctaacttta cgatcggttt agcaaaacag cttgcaccta aaggaatccg
     5761 tgtaaatggt gtagcacctg gtccaatctg gacaccatta caactagacg aaggacaacc
     5821 ttctgattcg atccctgaat ttggtcagca aagtttacta gaacgtgcag gtcagccagg
     5881 agaattagca ccagtttatg ttttcttagc atcgaataaa gcaagttatg ttacagccca
     5941 aatttacgga gtaactggtg gagaagcgat caacttataa gaagttgatc gtttcacccc
     6001 tatgaggagg tgcgaaaatg aataaaggtg taaaaacgat cggagtgatc ggaacattac
     6061 ttttgctatc tgtgcttttg tttgcgatac tattaagtgc aacctatcca ttgccttttc
     6121 agctagagaa gtttcgattc ttttccatca ctaattcata cgtgcaacaa tatgtatttt
     6181 ggcttgccgt tgcatttgct attttgacaa ttatcacgtt acttgttttc ctgttttatc
     6241 caaaaacaat cggaaacttt ctactgaaac atggtgatgg ggagttgacg ttggataaaa
     6301 aagcaattga aggaatggtt cgttctcact tgcatgaaga tgaattcatc cattcaccaa
     6361 aagtaaatgt caaagcaacg aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac
     6421 gaacttcagc actcattggt aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac
     6481 aacacatatt gggaacaaaa gagccagtga aagtatcggt gaaatacaca ggctttgaag
     6541 aaccagctaa acgaagcaat gaccgcaaac gagtagaata gggggggacc aaaaatgaaa
     6601 gaactatttt cgacatacaa gctccctatc atctttgggg tacttggatt gatattagcc
     6661 gttttatgga tcaccgtggg ctttttcaag accttattga tcttgatcat ggcagcgatc
     6721 ggcgtcgcta tcggttttta tctgaaacaa acgaaaatct tagatcacta ttttaaatct
     6781 taggaggaaa catccatgaa taacaataac aaaaataata acaataaaat gaacaaccaa
     6841 aaaccagtgt ctgcacatcc agacccaacg acaccaaacc aatcaaatgg tgtgaacgat
     6901 gaaaagaaca aaacagcacc agtatctgca catccagatc cagtagtccc taacgttcaa
     6961 caaggagtag aagagaagaa acatgatgca gaaaagcatg cacctcatca tccaggaact
     7021 ccagaatcta aagttccaga acaacatgat gcaatcgatc cttctaaacc tgtggaagag
     7081 caaaaacatc cttctgacca tgcgaaagca caagaaaaac ttagttctga ccacacaaac
     7141 ggacaaggaa aacttggttc tgatcataca aataaccaag gaaagcatga ttctgaacag
     7201 gaagattcaa tgcgcggtga gctgaattat gaagataaag ttgtgcaaaa aatcatcgga
     7261 atcgcaattg aacaagtaga cggtttatta tctgcaaacg gtggtttctt ctctaatgtg
     7321 gcaggaaaac ttgtgaatac agataatgta acggctggga tcgaaacaga agtcggtaaa
     7381 aaacaagtag cagttgatat ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc
     7441 tttgaagaaa tgcaagaagt aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg
     7501 atcgaagtca acgctaacgt agtggacatc aaaacaaaag aagaatttga aaaagatcaa
     7561 gaaacggtgc aagacaaagt aactgacgca gcgaaaaaaa ccggtgaatt tgcttcgaat
     7621 caaacagata aagtaaaatc agcagctgga agcggcgcac aaaaagtaaa agaaaataca
     7681 gaaccacggg ttcagtaata aggaagtaaa acattaggag gaacgaaaca tgggatttat
     7741 ttggtcactt atcgtcggag gaattatcgg agcaattgca ggagcaatca cgaataaagg
     7801 ttcttcgatg gggattattg caaatgtcgt agcaggttta gttggatcag cacttggtca
     7861 agcattatta ggttcttggg gaccaagtat tgcaggaatg gcaatcgtgc catcattgat
     7921 tggtgccatc attttagtac taatcgtatc attctttgct cgaaaactgt aaaaagactt
     7981 ttaaaaagag gtcgtgaaaa agctgttct
//
LOCUS       DACEMO010000121         7954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEMO010000121 DACEMO010000000
VERSION     DACEMO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.124"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(449..1795)
                     /gene="gor"
                     /locus_tag="IW728_001977"
     CDS             complement(449..1795)
                     /gene="gor"
                     /locus_tag="IW728_001977"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1025327.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1929..3170)
                     /locus_tag="IW728_001978"
     CDS             complement(1929..3170)
                     /locus_tag="IW728_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1025328.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3188..4111)
                     /locus_tag="IW728_001979"
     CDS             complement(3188..4111)
                     /locus_tag="IW728_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1025329.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4332..6824)
                     /locus_tag="IW728_001980"
     CDS             complement(4332..6824)
                     /locus_tag="IW728_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1025330.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6831..7298)
                     /locus_tag="IW728_001981"
     CDS             complement(6831..7298)
                     /locus_tag="IW728_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1025331.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7434..7507)
                     /locus_tag="IW728_001982"
     tRNA            complement(7434..7507)
                     /locus_tag="IW728_001982"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7471..7473),aa:Arg,seq:acg)
     gene            complement(7518..7603)
                     /locus_tag="IW728_001983"
     tRNA            complement(7518..7603)
                     /locus_tag="IW728_001983"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7567..7569),aa:Leu,seq:taa)
     gene            complement(7620..7691)
                     /locus_tag="IW728_001984"
     tRNA            complement(7620..7691)
                     /locus_tag="IW728_001984"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7657..7659),aa:Gly,seq:gcc)
     gene            complement(7703..7775)
                     /locus_tag="IW728_001985"
     tRNA            complement(7703..7775)
                     /locus_tag="IW728_001985"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7740..7742),aa:Thr,seq:tgt)
     gene            complement(7793..7874)
                     /locus_tag="IW728_001986"
     tRNA            complement(7793..7874)
                     /locus_tag="IW728_001986"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7838..7840),aa:Leu,seq:tag)
     gene            complement(7882..7954)
                     /locus_tag="IW728_001987"
     tRNA            complement(7882..7954)
                     /locus_tag="IW728_001987"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7919..7921),aa:Lys,seq:ttt)
BASE COUNT         2175 a         1774 c         1313 g         2692 t
ORIGIN      
        1 atttgttcgg aaaaagtgac taactatgac acaatttttt tcacagtccc ttttttcact
       61 tgatttgatt cgtaccagcg aacttgacga gaactttctt ggtttggtaa ctatatggta
      121 ttcttttaac atcctcgttt gcttcttcta atttatttat acaaagtcac acttttttaa
      181 acatctgttc ttcgatacat ttctatttct tttaaacatc gataccaata ttttgaactt
      241 aagagtgcat accaaatgga ttcatttact cttcgtcagt tacgaagata atcaaaatct
      301 ccaacttccc tacttatttc gttttgagaa ttatcgaagc aatcttacat aaataactaa
      361 aaaacaaaaa accagaaaat cagtatactg attttctggt tttacatggt ttgttcaata
      421 aaaagctgtt ggaactgtta atattaaatt atcgcatcgt tacgaattct tccgctcctg
      481 ttggatggat ggctactgta ttgtcaaagt cttctttagt tgcacccatc ttgatagcca
      541 cggcaaaacc ttgcagcatc tcatcgacac caacaccaat agcatgtaag ccgacgattc
      601 tttcttcttt tccgacacag atcaatttca tttcgcattt ttggcgataa tcatttaatg
      661 caaagtacat tggggtaaag cgtgaacgat aaactttcaa ttcattttcg ccatattttt
      721 ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg tggatgcgta aagacgactg
      781 tcggaacaag tttatagtca agataagaat ctttctttcc attaaataaa cgttctgata
      841 aacgtctacc agctgcgatt gcaacaggag tcagatctaa tttaccgatc acatcaccaa
      901 cagcaaaaat atgttcatcc gttgtatttt ggtatttatc tactttgata aacccttttt
      961 tgtccaattc cacatttgta ttttccagac ctaatttttc ggtattcggt acacgtccac
     1021 cggcaaaaag tacacaatca ccagttaagg tttcaccatt ttcaaatagc accgtatatt
     1081 cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc gtaagttttt attccagctt
     1141 cttggtacat ttggatcaaa ttgtccgaaa gcattttatc aaacgttcta agtggtcgtt
     1201 cttttcgata tgcccacatg acttcactac ctaaaccacg aaatacacct gaaagttcag
     1261 ccgcaatgta tccagcacct aaaacgattg ctctcttagg taactctgtt aattcaaaaa
     1321 atccatttga atcaattgcg tattctccac caggaatggt cattttactt gcacggcctc
     1381 cagtagcgat caagatttta ggagcacgat aggtttcacc gtttacttcg atcgtttgac
     1441 tatcgataaa agtagcgtaa ccttttatag cctcgacatg attgctatct aatcctcttt
     1501 gataagcacc gtgtagaaaa tcaatatatt tttctcgtct ttctactaga ccggcgaaat
     1561 caacattttt taaatctgct tggataccgt aacttgaggc atctcgttca attgtttcca
     1621 aaatcgtact tgcttgccac ataacttttt taggtacaca accgacgttg acacatgttc
     1681 cgcccaattc attcgcttca atcagcaaaa cttttgcgcc atgcatccct gctctgtttg
     1741 ctgaagcaat accgccagag ccgccaccaa ttacgatgta atcatatatt tgcattttgt
     1801 tcctccttat tgaagagatc aatcaaaacc tttatttaac gatccttttt ctacagaaaa
     1861 caagtgtaac acttttcttg aattttgtat gcgaagatgc ttaaaacagg gatatatcag
     1921 ctttttcctt atttacgttt tcggatcata tctccaggtt tcaccggctg tgcgactggc
     1981 atagttaaaa tcatcattgg atttggtgct cggtcaatgg attcgccttc ttcattatgc
     2041 ataacttcaa cggtttgact gaaatgactg aatcctggac cataaaattc gatttcatct
     2101 cctacagaaa acaagtttct ctgacgtatc gtagcaatct gtgttgcctc atcataagat
     2161 aatacttcac cgacaaattt atatactggg attttacgac gttcaccgaa taattgttcg
     2221 ttttcagttg gtgtattgta gtaaaaacct gtagctaatt ctcgttgtgc tactttccat
     2281 aactcgtcga tccattcttg cttgcagaca tagttttcag gatcttccat ataactgtct
     2341 acagccgctt tatagacatt tgacaccgta gatacataat ggatcgattt cattcttcct
     2401 tcgatcttga agctgtctac gccgttttca atcaattctg gaatatgttc gatcattgac
     2461 atatccactg cactcatcga gaattcttct tccacttcgc ctgtatcagt taaactgttt
     2521 cgttcttgac caaaaggcat atcgaacaat ccatacttcc agcgacaaga ttgagaacat
     2581 ccgccacggt ttgcatcccg catcgacata tggttggaca atgtgcatcg accagaataa
     2641 gagatacaca tggcaccatg gataaatgct tcgatttcgg tatccgtatt tttacgaatc
     2701 tctgccactt cctccattga aacttcacga gctaaaacga cacgttccaa cccttcttct
     2761 ttccagaatt ccaatgtctc aaaattagtt gctgaagctt gagtggataa atggatcggc
     2821 agacctgggg cttctgtcgc acaaatttcg atcaaagctg gatcagaaac aatcactgca
     2881 gaaataccga catcacgcaa actgcggaaa aattcccctg ctccatcttc atttccttca
     2941 tgggtgacca tattagcagc aacgtatacc ttcgcatcat gttcttttgc aaaagcaact
     3001 ccttcagcca tttcttcata agtaaaatta cctgcacggc ttcttaaacc atatgcattc
     3061 ccgccaatat atactgcatc tgctccataa tggatcgcgg tttttaactt ttccagtgtt
     3121 cctgctggcg ctaggatttc tggtcgtttc aattttcttt ttactgtcat ttctttcatc
     3181 atactcctta tttgatagca gaaggatcaa tataaaagaa tcctgtatct aattcacggt
     3241 tttcaggatg caatttactg atttcgctag tcattttttc cgctttttct tgatcccagc
     3301 taccttctaa aatttgtttt tttgcttcgg cgaatagtcg agtgatgttt acgaattttt
     3361 cgcctggagt aaataatccg tccaatttcc atgtacagta atgatgctga taaagttcat
     3421 tcaattcatt cgagagattc agatcattgc tggcaaaaat atgtgtgcca tgactatctt
     3481 cataaataga ataatgtgtc tcttcttttt taggttccga gataaaaaga cctctttcac
     3541 gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt ctgtagcagt ggacgtttgg
     3601 attgatggat gcatgtagca ccatacacaa gggtttctac aggaatatcg agtttttctt
     3661 ccatcgcctt catctcttca aaaggaactt cacgagcaag tactgctcca actgcgcctt
     3721 ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa tgtttcagca tcataaatat
     3781 acggtatacg caattcagga ttttttttca tcgtataaat aatacctgta tcaccaactg
     3841 tgatcacatc tacgtttatt tcttttaaaa aagccaaata ttcaggtaca tttttcattt
     3901 tttcaggatg aatgatccca tttacagcaa tattcgcttt tttaccatac ttgtgtgcta
     3961 attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc aggcagacgc aatccaaatt
     4021 gctcttcacc gaaatatagt gtatctgcac ctgcagcaag caactgttct gcttgttcaa
     4081 tagtttctac tgtggttatg agttcgatca taattttctc ctcctacaac ttcataatag
     4141 tagcatagct ctaaaaactt tccaatactt cttctattta aagtcggatt tttgaaaatt
     4201 atttcacaaa aaatcaaaat aaagttcgat atattctgtc caaatataaa aagagattgt
     4261 gacaaaaatc ttgtcacaac ctcttaatca agtcttacta ttataaaaat tcagctacac
     4321 aggccaagta actataggga acctactgtt tcctttttag gtccgcgaac attcaatgtg
     4381 atcttacctt ttgaagcacc tactgttacg tggtcaccta aacgaatttc tccgccaagc
     4441 aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg cgcgacggat tggacgtgca
     4501 ccatattcag gatcaaatcc agctttcccg atcacatcga ttgcagatgg tgtgattttc
     4561 acttgaatat cttgttcttt caatcgtttg ataatcgctt tgctcatgat ttggacgatt
     4621 gtatggattt catctttact caatgaatgg aagacgacag tctcatcgat tcgattcaag
     4681 aattcaggtc ggaaagcttt ctttaactct tctaagatac gtttttgcat ggctttgtga
     4741 tctttagtaa catcttggac attgaatccg acattttttt cttctcgaat ttgtgtcgct
     4801 ccgatatttg aagtcataat catgattgta tttctaaagt ccacttttct acctttagaa
     4861 tctgttaaat gaccatcatc caatacttgc agaagtaaat tgaaaacgtc tggatgtgct
     4921 ttctccactt catctaagag aatcactgaa tatggttttt gacgaatttt ttctgtcaac
     4981 tgtccacctt catcataccc tacgtatcct ggaggagacc cgattaatcg gcttgtactg
     5041 tatttttcca taaattctga catatcaaca cggatcaggg catcttcact tccaaacatg
     5101 acttctgaaa gtgctttggc caattctgtt tttccgacac cagttggtcc aaggaacatg
     5161 aatgaaccaa ttggacgatc aggatctttc aatccgcttc gtgcacggcg gatcgcacgt
     5221 gaaactgctt tgacagcctc atcttgacca acgacacgtt catgcagaag tccttccagt
     5281 tctaacaagc gttcgctttc ttttttctcc aactgctgta gagggacacc agtccactcc
     5341 gatacaactg tagcaacatc ttcttctgtt acacgatcag aataaccttg ctcctgtttt
     5401 gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg ctttttcttg tctgcgtaat
     5461 tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt ccttttcttg tactagttca
     5521 tcgatttcta gtttgatcac agttgatttt gttaagtgat cggtttgatc caaacggact
     5581 ttcgctgctg attcatcgat caaatcaatt gctttatcgg gtagttgacg agaagtgata
     5641 taacgaacag aaagattgac tgctgctcgt acagcttcat ctgtgatctc cactccatgg
     5701 tgctcttcat atcttgtacg taatccttga agaatcactt ctgcttcttc aggagttggc
     5761 tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag aatctttttc gatatatttt
     5821 tgatattcat ctagggtcgt tgctccgatc gtttgtaatt ctcctcgagc taacgccggt
     5881 ttcaagatat tcgaagcatc aattgctcct tctgctccac ctgcaccaat taaggtatgc
     5941 aattcatcaa tgaataagat tacttgacca tcattataga tttcgtcgat gacctttttc
     6001 attcgatctt caaattcacc gcgatatttc gtaccagcaa ccaaagatcc catatcaagc
     6061 atcatcaggc gcttttcttg catgtcttct ggcacttcac ctaaaatgat tttttgagct
     6121 aacccttcag caatggctgt tttgcctaca ccaggttcac ccaccagtac agggttattc
     6181 tttgtccgtc ggctaagaat ctggataagc cgtttcactt ctctcgaacg accaacaact
     6241 gggtccagct tgttttcttt tgctaatttc gtcaaatcac gtgctaatga atctagcgta
     6301 ggtgttcctt gcggtgtttg ccgtttatct tgtacattta catttctacg acgattcatt
     6361 ccttttccag cagaatcacc agctagcccc attttttgtt tcagaacttg acgggctttt
     6421 gaaaggctga tacctaagtt gatcatgatt cgagtagcca tgatactttc atcttttaat
     6481 aagctcatca aaatatgttc tgttccaact ttttgagaac cagttttctt cacttcgctg
     6541 ctagctaaag caaaaacttg tttcattctt ggtgaatatg gaagaaagac tcctttaggg
     6601 taagaacgga taccaccata accaatcaga tgttcaattt cttcataaat ttccgtttca
     6661 ttcaaatcca tttcacgtaa tacttttcca gcaattccat tttgctcgac cactaaggct
     6721 agtaaaacat gttctgaact aactgattga tgtttcaacc gttttgcttc ttcttgtgca
     6781 atttctaata ctgcttgggc tctttcagta aaaagttctt ccatctaatc tcacttccca
     6841 tcttcataac ttaaacgttc cagaaatgct cggagaatcc gagcacgtgt atgatcttca
     6901 tattcattga aattcaatgt gttttttgca attgcactca acatcaagtt tccttctttt
     6961 tttgtaatta tttggtcttc ataaagtttt tgaataatag caaaagcatt tttctcactg
     7021 attgtatcat caaataactg atttatctgc tcaagtaatt gttttttatc tgaaatcctt
     7081 acttttgcaa ttcgtatata accaccacca ccacgtttgc tttctactag ataaccacgt
     7141 tggatggtaa aacgggtatt aatcacatag ttgatttgtg aaggaacaca gttaaagagg
     7201 tctgccattt caatccttcg aatctcgatc atttcactat tttccagaat tttctttata
     7261 tatgattcaa ttaaatccga ggtattctga tgtgccatct tattcattcc ctttgactaa
     7321 tattgacctt aattataacg aatttgataa tagaaagaaa gaagaacgct ttttgttaaa
     7381 taaatccttt agaatcaaca aaaaaaaata cggaatatac tattccgtat cgacgcgccc
     7441 aagaggagtc gaacccctaa ccttttgatc cgtagtcaaa cactctatcc agttgagcta
     7501 tgggcgcata tttgaaatgc cgaggaccgg aatcgaaccg gtacggtgat cactcaccgc
     7561 aggattttaa gtcctgtgcg tctgccagtt ccgccacccc ggcgcgattg ctttggcaaa
     7621 gcggaaaacg gggttcgaac ccgcgacccc caccttggca aggtggtgct ctaccactga
     7681 gctatttccg cgtgtttaaa gatgccggct aaaggacttg aaccctcgac cctctgatta
     7741 caaatcagat gctctaccaa ctgagctaag ccggcatgta aaacttaaat tatgcgggtg
     7801 aagggacttg aacccccacg ccgtaaggcg ctagatccta aatctagtgc gtctgccaat
     7861 tccgccacac ccgcaaaaat atgagccgta cagggctcga acctgtgacc ctctgattaa
     7921 aagtcagatg ctctaccaac tgagctaacg gctc
//
LOCUS       DACEMO010000122         7944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEMO010000122 DACEMO010000000
VERSION     DACEMO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.125"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            382..1755
                     /gene="glmU"
                     /locus_tag="IW728_001988"
     CDS             382..1755
                     /gene="glmU"
                     /locus_tag="IW728_001988"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1025332.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            1849..2820
                     /locus_tag="IW728_001989"
     CDS             1849..2820
                     /locus_tag="IW728_001989"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1025333.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      2826..2928
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2994..3563
                     /gene="thiT"
                     /locus_tag="IW728_001990"
     CDS             2994..3563
                     /gene="thiT"
                     /locus_tag="IW728_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1025334.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(3644..4417)
                     /locus_tag="IW728_001991"
     CDS             complement(3644..4417)
                     /locus_tag="IW728_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025335.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            4684..6384
                     /locus_tag="IW728_001992"
     CDS             4684..6384
                     /locus_tag="IW728_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1025336.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            6354..6572
                     /locus_tag="IW728_001993"
     CDS             6354..6572
                     /locus_tag="IW728_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025337.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            6592..7482
                     /locus_tag="IW728_001994"
     CDS             6592..7482
                     /locus_tag="IW728_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1025338.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            7500..7784
                     /locus_tag="IW728_001995"
     CDS             7500..7784
                     /locus_tag="IW728_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025339.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         2693 a         1364 c         1669 g         2218 t
ORIGIN      
        1 agaggttgtg aaatcagcgt cctgtcctga gtattagctc aataaacgga aaaattgctc
       61 ctcatatttt gccgtttatt gagctaatac cgaaggtcag ctgattgaaa aacgtttaat
      121 gaagaggttg taagcctacc gtttaactct aagtcacaag aaacgatttc aaaaaacagc
      181 ttttcatgtt ttatgagtag tgtgacttgt gaccgaggag ttggcaggag agcaccggtt
      241 accaaataga taggcccgtt atggtctatc tattttttta tccagaaaac gctgggataa
      301 aagaaaatca cttgttttgt tgaaaaaatg ggtatactgg gataaactga aatcagcaaa
      361 ctatatttag gagtgacgca gttggacgca cgttatgcaa ttattttggc ggcagggaaa
      421 ggtacccgca tgaagtcaaa attatataaa gttttacatc ccgtttctgg taaacctatg
      481 gtagaacata tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat tacgatcgtt
      541 ggacacggag cagaacaagt taaagcacaa ctaggagaac gcagtaaata tgcgttacaa
      601 gcagaacaat tgggaacagg acatgctgtt ttgcaagctg cttctttctt ggaaggaaaa
      661 aaaggaacaa cgttagttat cagtggcgat acaccattat tgactactga aacgctgaac
      721 aatctttttg aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac ggcacaagca
      781 gaaaacccaa caggatatgg acgaatcatc cgtgaccata ttgggatcgt agaaaaaatc
      841 gtggaacaaa aagatgcaac accagaagaa gctcttgtac aagaaatcaa tacaggaact
      901 tactgttttg acaacgaagc tttgttcgat gcgttaagca aagttggaac aaacaatgct
      961 caaggtgaat actatctaac agatatcatc gaaatcttaa aagaagaagg ccatacagtt
     1021 gctgcttatc aaacggatga ttttgaagaa tcaatgggag tcaatgatcg gatcgcgtta
     1081 gctaaagcaa atgagatcat gagaaagcga attaaccaga tgcatatggt caatggcgta
     1141 agctttgtcg attcagcaac gacatacatc gatgcaggcg tagaaatcgg tccagacaca
     1201 ttgatcgaag caggtgtaca aatccaagga aatacagtca ttggttctga ttgtgtcatc
     1261 gggtcacatt caaaaatcgt agacagtagg attgaggatc acgtcgtcat tgaaaactca
     1321 gtcattgaaa gcagccatgt caagaaacat gcagatgtgg gaccttatgc acatttacga
     1381 ccaaaagctg aaattggcga aaacgtccat atcggaaact ttgtggaagt gaaaaatgct
     1441 caaatcggca aaggaactaa agtcggtcac ttgacttatg ttggtgatgc tacgctggga
     1501 gaagaaatca atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa gaacaaacat
     1561 catacgactg tcggcgacca cagctttatt ggttctagca cgaatatcat cggacctgtc
     1621 gaggtagcta aaaacagttc gattgctgcc gggtcaacaa ttacagacaa tattccagaa
     1681 tatgcgctgg caattgcgag agcaagacaa gtcaataaag aaggatatgc aaaaaaactc
     1741 ccttatctaa actaagactt ttgcaaaatc acttgattta tttaaaaaat atgactacta
     1801 tttattaggt agaattctag agttgtgaaa aagaaagtgg aggtccccat gtcaaaacat
     1861 tactttgacc caagattaaa aatttttgcg ttaaattcca atcgcccatt agcagagaaa
     1921 attgcagcag cagtcggcgt cgagttagga aaatcatctg ttactcaatt tagtgatgga
     1981 gaaattcaag taaacattga agaaagtatt cgtggatcgc atgtctatgt tattcaatca
     2041 acgagcagcc cagtcaatga taacttgatg gagttgctga tcatgattga cgcactgaaa
     2101 cgagcaagtg caaaaacaat caacgtagtc atgccttatt atggctatgc gcgtcaagac
     2161 cgtaaagcac gtgcacggga accaatcaca gcgaaattag tagcaaacat gattcaaaaa
     2221 gctggtgcaa caagaatgct gactttggac cttcatgcag ttcaaatcca aggtttcttt
     2281 gatattccag tcgatcattt gatgggtgcg ccacttattg ccaattattt cttagaaaaa
     2341 ggaatcaaag gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt cacacgggca
     2401 cgtaaattag cggaattttt gaaatctccg attgcgatca tagacaaacg ccgtccaaaa
     2461 gcaaatgttg cagaagtaat gaatatcatt ggacaagtca aaggcaaaac ttgtgtactg
     2521 atcgatgata tgatcgatac agcaggtacg atcacattgg ctgccaatgc tttgaaagaa
     2581 gctggtgcaa ctagtgtgta tgcttcatgt acacatccag tattatccgg ccctgcttta
     2641 caaagaatag aagattcagc tatcgagcgt ctagttgtta cagactcgat ctatcttcca
     2701 gaagatcgta aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat gggagatgca
     2761 atcaaacgta tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa aaataaataa
     2821 taaataaact cacaaagggg agtccaattg ggctgagatt gaatctattt ctaaaccctt
     2881 cgtacctgta tcggttatgc gagcgtagga attgtgaata aacagctgtt tgactatttt
     2941 cgaaagctgc ttcctccttt gtgacttttt tattcaaagg aggaagtttt tttatgggaa
     3001 aaaatagagt ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg ttgtcgttga
     3061 ttccgatcca gatcggcagc agtttttcta tttcattagg gcagattcca ttgactcttt
     3121 ttgcgttaag aagagggaca aaggcaggga tgctggcagg tttcatttgg ggcatcctac
     3181 attttccgtt gggacaagtt tattatttaa gtgtcgttca agtgttgatt gagtacccga
     3241 ttgcctatac ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa aaaggctatg
     3301 tagacgataa tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg attggcgcac
     3361 tttccagata tttttggcat tttattgcag gagtggtttt ctggggagcg tatgcactat
     3421 gggggatgaa tccttggctc ttttctttcg taatgaatgg tgccagcggg ttgagtacag
     3481 ctattgtaac gatcatcgtt ttattaggga tccaacaaac gactccttcg atttttgtgc
     3541 cagataaacg acaggagctg taaataaaaa agaagcaaga aacttgagac agaatcatgt
     3601 cctagtttct tgtttccttt ttattttttg ataagaaata gatctacttc aatggcagat
     3661 aagaaagatc ggcatcttct accaattgcc agtgtcgatc ggtgacagag tagattaaag
     3721 tagcgatgat ttcctcttct cgataccggg tgccttttaa tttccaaata taatcttcaa
     3781 tcacatagat tgggatacga tatttaccat cgatcaaacc aacttgatca aaagaaaaaa
     3841 tgatcccttc ctgcatcaaa ttacgcattt ctttcaataa ttccacactg attttttcaa
     3901 caggtgtttc ttttttgaaa aaagattttt ttacattgat cttatctgac aagatcgaac
     3961 ttaatttact agcaaaaatc ctataaaatt catctaaata gcgataataa atccgattca
     4021 gctgatcatt aagatcgaag tatacaaaat tattctgtag tttataaaaa aacggcgaat
     4081 gaagatgtgt tttcatatga ccaaaatata ataattctga aatttctaaa ggtgtcaatt
     4141 ctttcagcat cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat ccacgcctgt
     4201 tacgagaaga ggaaaagaaa cgctgaatat tttctgtacc acggattact ttcagccctg
     4261 tatgggattc atattctcca gcagaagcag atggatcaag caagagcaag ttttgaggaa
     4321 aatgcacgat actgtttgca aagtcagtag cagtcagccc ttttgttaaa acagcattac
     4381 ttgtggtatc aatatgtaca taaactgact cagtcatcat gttcctcctc ttatttcctc
     4441 ttaatctctt ttattttaca acatttgcat taaaaaagct tgtttctttt attttaaaat
     4501 tccattacaa aatcacgttt tcattgaaaa cactctcatt ttcaattaat ttttgtgatt
     4561 tttgtatttt tttatttaaa ggaggtttta tgaattgttt tcttatggaa tctacaaagt
     4621 cgaatacgag actcatgacc tttttatgac gtactggtat acttgaagaa aaaggagagc
     4681 gcgatggaga taaaaaaagt ttacttttca cagaaaatgt cgctgtattc gcctatccag
     4741 caagcaaaac aaccccttcc attttttcaa agtcatcaag aaagtagaga cgctttttgg
     4801 cgttctgcaa aaaagcaatg tgcttttaaa gcagtatcga aaaaatatac cgtctatttt
     4861 actattgcta aaagtagagg agatgttatt tttaataatc tgttgattga aggtgaaact
     4921 tttgaatgga aaaaattttt tcggtatatg gaagcttgcg ctcgtttttt tattaaagaa
     4981 aactgttgtt ttttatttca atctcctcta tcagaagaat ggttgcgtat ttttcataaa
     5041 aacggctatc aaggaaccac acaattaaat aaaaagctgg tctatcatac agcacttgtt
     5101 ttaggtggag gaggagcgca cggtgcttat cagattggtg tgtggcaggc gctgaaggaa
     5161 catgatatca actttgagat tatcacaggt acctcggtag gtgcgttgaa cggcgcattg
     5221 attttacaag gagatatgaa aaaagctgtg aatttatgga aaaagctgtc gacaagacaa
     5281 gtgcttgcgt taccagaaat ggctgcagtt gaagaccttc gtgaacgctt ctatcgagaa
     5341 agacgccaaa tgacgaaaac agcactaatt gaaggcgggg tttcttctgc tccattggaa
     5401 aagatggtca aagacaattt ggatttgtcc ttactcaaac ataatcccaa aatacgttta
     5461 tatacggtgt ctactaaact gccagaacta gcggaagtag tgactgatat ccaacagaca
     5521 aaaacagaag aaataccaga ttggatactc gcctctgctt cattttatcc cgctatggct
     5581 tatcgaaaga tcggaaaaaa taaatatgca gacggtgggt atcgcaacaa aatacctatt
     5641 gatattgcga ttaacaaagg agcaacagag gcatttgtcg tagatgtgca agggccaggc
     5701 ccagcgaaaa aaattcgtat gccagacatt tttatccatt ggaaatgtca aacattgtgg
     5761 acactaggca gctttctgct ttttgatagc caaaggaacc aattaaattt acaattaggc
     5821 tatctggaaa tgaaaaaacg attaggctat tactatggga actggtatac atttcattct
     5881 gttaaacctg caagaacata ctggcgagga tttttgtctt acttaacaaa agaaattcag
     5941 ttagaattat cttttttcaa aagcataaaa ttttggcaaa aactgcgtaa tttatataag
     6001 aacagggtcg taccagagac atgcggattg gcgatgctag aacttttagc aaaaaaacat
     6061 tttttactgc ccaatgaaat ctatcaagta gacatgatga ttcaaatgat ctgccaacaa
     6121 gatataaaaa gtatgccgga tgatttattg gcacaaattg gtcaattaag tactgaagaa
     6181 tggcgaaggt atcgaaaata ccagcaaaaa atacaaaatg agcgcacgaa aactgtttat
     6241 tttgattatc tgctacaagg aaaacgcaag gatcgtttgc agcatgaatt gcttaagcaa
     6301 ccagttgaca cattactgat cctatattta tattatctca aggaggaaca accatggcac
     6361 aagaattttc atatgaaatc gtagaagaaa tcgctatatt gtcagaaaac aacaaggggt
     6421 ggcgtaaaga actgaaccta gtcagctgga atggtcgccc gccaaaattc gatcttcgag
     6481 attgggcgcc agaccatgaa aaaatgggga aaggcctgac attgacgaac gaagagtttg
     6541 aacaactgca aaaagcaata gaaaatatgt aatgaggaag gaccctgtcg aatgaaaagt
     6601 atgactggtt ttggcaaagc catccgcgaa acaaaagcgt atcagttaga tattgaaata
     6661 aaaagtgtca accagcgttt tctagatgtt caaatacgaa gtcctaaaat cctgaacttt
     6721 gttgaaaacg attttcgtca gcttattaaa cagcagttaa gccgcggtcg aatcgaagta
     6781 tttgtgaatc tagcttatat tggcaaaaac gaaaaacaga tcgtcatcaa ctgggaattg
     6841 attgattcac ttatcactca attgacagac ggaattcaac aacgatacgg tagtcaaaat
     6901 caagtgtcaa ttagtcggat ggcggaacgc ttcgcgctgg acgaaaactt cgtaacggta
     6961 gaagaaaaac cggaagagag catggaagaa ttggcacagt tagctctaga tacattgaaa
     7021 agcgcacttg attctattga ggcgagtcga gaaaaagaag gtcaagcttt agctttagtg
     7081 ttgaaaaaga atactgaaga attcaaagaa gtactaaaaa aactcgatag ttttgtagaa
     7141 atctatgaaa aggaatttca aataaggtat caaaaaaagc tggaggaatt tcttggagcg
     7201 aaagtcgatg agcaacggtt gttgacggag ctagccatct tgttggaacg cggagatatt
     7261 cacgaggaac tggatcgtct ggcgatccat gtacaaaaac tagatgaact tatcgaagca
     7321 caatgtcctg taggtcggga attagatttc ctgatccaag aaatgaatcg ggaaatcaac
     7381 acgatcggtt ccaaatcaaa tgcaatcgaa attaaaaatc aagtcgtcca gtctaagacg
     7441 atcttagaaa aaatcagaga acaagtacaa aatattgaat gacgaaagag gtagagataa
     7501 tgaaatatta tgacgtaacg ttccacgaat taagtggaaa aactgtggtt aaacgggata
     7561 tcccttctga aaaaaacggc tttgacgtat ggaaagatgc ctgtgcagac tataatgaga
     7621 acgaactgtt tattttgatc aacgacggtg cttacgtgac gatgaatcgg aaatttattg
     7681 ttcgaattga cacagaagag gtagaagatc cgacagaaaa agcacgttct cgaaaagatg
     7741 agatcatggg tgtagtgaat actttgtcca atatgggctt ttagcaagga tgctgaacaa
     7801 agaaatacaa taaataatca gaattggatt tcttcgctta gcttctttgc tctaggaata
     7861 catttaaagg ttgtgacaaa attcgtgtca caatcttttt ttattttgaa taagtgatgt
     7921 tcaaaaacca ttttctcggt atta
//
LOCUS       DACEMO010000123         7904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEMO010000123 DACEMO010000000
VERSION     DACEMO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.126"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            107..970
                     /locus_tag="IW728_001996"
     CDS             107..970
                     /locus_tag="IW728_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1025340.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IW728_001997"
     CDS             complement(1099..2721)
                     /locus_tag="IW728_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1025341.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IW728_001998"
     CDS             2970..3491
                     /locus_tag="IW728_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025342.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW728_001999"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW728_001999"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1025343.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IW728_002000"
     CDS             5572..6390
                     /locus_tag="IW728_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1025344.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IW728_002001"
     CDS             complement(6493..6690)
                     /locus_tag="IW728_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025345.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IW728_002002"
     CDS             6859..7437
                     /locus_tag="IW728_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1025346.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         2381 a         1397 c         1571 g         2555 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aata
//
LOCUS       DACEMO010000124         7882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEMO010000124 DACEMO010000000
VERSION     DACEMO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.127"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..163)
                     /locus_tag="IW728_002003"
     CDS             complement(<1..163)
                     /locus_tag="IW728_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003492450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1025347.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFT"
     gene            complement(583..1704)
                     /locus_tag="IW728_002004"
     CDS             complement(583..1704)
                     /locus_tag="IW728_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ1025348.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2032..3441
                     /locus_tag="IW728_002005"
     CDS             2032..3441
                     /locus_tag="IW728_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1025349.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3438..4166
                     /locus_tag="IW728_002006"
     CDS             3438..4166
                     /locus_tag="IW728_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1025350.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4294..5205)
                     /locus_tag="IW728_002007"
     CDS             complement(4294..5205)
                     /locus_tag="IW728_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1025351.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5222..6229)
                     /locus_tag="IW728_002008"
     CDS             complement(5222..6229)
                     /locus_tag="IW728_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1025352.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6226..>7882)
                     /locus_tag="IW728_002009"
     CDS             complement(6226..>7882)
                     /locus_tag="IW728_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1025353.1"
                     /translation="YTGLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFS
                     SDVSEAALSLGTKIVVPNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGE
                     GRVNKILSVSPDENKGKDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGIS
                     FFVFLLTGIMLFSQILFIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVT
                     LVITTAFSISTMFFNISATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMG
                     RRVMRKPQMLMNRKLRQLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRK
                     NHSARSINELNSDLKKQEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVT
                     DAPTNLKYSLHKGMEKTKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLN
                     EAWNRHEKGRENVPIKKEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGS
                     TQPLSKNNSFQQVKQRFTFPKRTNQQVQRKKNTRPKPKPGEKR"
BASE COUNT         2396 a         1573 c         1390 g         2523 t
ORIGIN      
        1 tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta tatcctcgtt
       61 tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc ataccagttt
      121 ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt ccttgatggc
      181 tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta tacaacaaca
      241 gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat gctcactgat
      301 gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg tccggaaagc
      361 ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga aaaatctatt
      421 tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt tcttgctaaa
      481 aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa attttagtat
      541 gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag gtttgtaacg
      601 tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt tcaattgaaa
      661 cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg ttgcgattaa
      721 ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc gtttaatggt
      781 cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt gatacatgtc
      841 ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga ttttttgtcc
      901 cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac cgccgacaag
      961 aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg ctacttcaat
     1021 ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga aatagccgtc
     1081 acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca catacgttgc
     1141 ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg catttgtcat
     1201 cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca aatgtagctt
     1261 gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt tcaacggaat
     1321 agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct gagcagaaat
     1381 tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt ctggcgctct
     1441 acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga ggcgctgatc
     1501 taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg catgaagtaa
     1561 cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt cgctaattaa
     1621 gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt tttgaaatga
     1681 ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg ataagtcatc
     1741 cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc ttttgatggg
     1801 ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa agatatttta
     1861 ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa tttaactaca
     1921 cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact ggtttccttc
     1981 catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac tttggatgat
     2041 attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca gataagaaaa
     2101 gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca actattcatt
     2161 acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga aatcatcggt
     2221 agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc caaagctatc
     2281 catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg tgtatacatt
     2341 tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga tcgtcttgat
     2401 catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca tgaagatcac
     2461 atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac gtccgcaagt
     2521 gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact atttaatcaa
     2581 tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt gaaaaaaaca
     2641 aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac cattcattat
     2701 ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca tgtcgaaaat
     2761 aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga ctcttttgag
     2821 tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac gcattattca
     2881 aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca ttctcttcca
     2941 caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta tcacgaaatg
     3001 aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca attgactgtt
     3061 gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt aatgaacgaa
     3121 tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat gaaacaatgg
     3181 agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc tgcacgcctt
     3241 caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa acaattacta
     3301 agtttattag cgacctacga tatccaacaa attaatgaac aattttacga attagtcgtt
     3361 caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc attaacaaaa
     3421 acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa aagcttcgat
     3481 taagccatat catcacgaac ataccaagag cttctttcga agctccagaa caatttatta
     3541 ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa cggatggaaa
     3601 aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac gaagatattt
     3661 ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta aaaaggcaag
     3721 aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg atcgcattag
     3781 ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac aatctcgtag
     3841 agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag cgattcgaaa
     3901 caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa gaaggagctg
     3961 aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt attacctcta
     4021 atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca gctgctctcg
     4081 ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt ttcagattga
     4141 aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa ctctgcactt
     4201 tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac gacgtaattc
     4261 caaatgttgg attgctttta gataatcttt tatttattca caattgctaa tagtccaatt
     4321 ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg acaactcatc
     4381 aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt aaataggatt
     4441 cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt tttcaacata
     4501 atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct ctaaaaactc
     4561 ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt gtcttacttt
     4621 atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa tatgattgcc
     4681 tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg tctttttccc
     4741 ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt gtttttcaac
     4801 atctagaatt tttactgata caacctcgga actagtggga atatccgctc gaaccatatc
     4861 ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca ttctcttttc
     4921 aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta cagtaaaatt
     4981 ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat gagttgtatg
     5041 ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag acaataaaag
     5101 ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt ctttcttctt
     5161 ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc attcctcttt
     5221 tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta tcggtaagat
     5281 tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca taaatgccca
     5341 catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa tcgcctggtt
     5401 tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc acccgaggga
     5461 gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca aacccagttc
     5521 cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc tcgtcaaaaa
     5581 ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc actaataagc
     5641 aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc gcgatgggat
     5701 tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg gctaaagcaa
     5761 aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca tagttataag
     5821 cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc aaagacgaaa
     5881 aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt gataaacctg
     5941 ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact tgaataatcg
     6001 ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt tccaccattg
     6061 aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt ccagaactac
     6121 cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg cccacaaaag
     6181 agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc ttttctcacc
     6241 tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg ttcgctttgg
     6301 aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg gctgagtgct
     6361 tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat tcgaatgtac
     6421 ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg gttttatctc
     6481 cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt cgttcaatac
     6541 tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc gcaactcccc
     6601 tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt ttttcgtctt
     6661 ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta cttgattttt
     6721 tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc caacttttct
     6781 accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt gttttttcag
     6841 atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg acactctatt
     6901 gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac caactgctgc
     6961 accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca attttcgatt
     7021 catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat cattactttg
     7081 aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa aggtcacaat
     7141 ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa acatcgtgga
     7201 gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa tcgtattgaa
     7261 caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg acaataagaa
     7321 actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca acataatccc
     7381 tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa agaccacggt
     7441 tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat cttcaatctc
     7501 tgctttgact gcttcttccc gatccttacc cttattttca tccggactca cggataaaat
     7561 cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt gtaatagtaa
     7621 ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca cactatcttt
     7681 tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag ccgcttcact
     7741 aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg caatgaaaga
     7801 acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt tggtagtttc
     7861 tcgttttaat aatccggtat ac
//
LOCUS       DACEMO010000125         7796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEMO010000125 DACEMO010000000
VERSION     DACEMO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.129"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            137..1456
                     /locus_tag="IW728_002010"
     CDS             137..1456
                     /locus_tag="IW728_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ1025354.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1763..4204
                     /locus_tag="IW728_002011"
     CDS             1763..4204
                     /locus_tag="IW728_002011"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1025355.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4534..5238)
                     /locus_tag="IW728_002012"
     CDS             complement(4534..5238)
                     /locus_tag="IW728_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025356.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(5425..5670)
                     /locus_tag="IW728_002013"
     CDS             complement(5425..5670)
                     /locus_tag="IW728_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025357.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(5687..6538)
                     /locus_tag="IW728_002014"
     CDS             complement(5687..6538)
                     /locus_tag="IW728_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1025358.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            6704..7708
                     /locus_tag="IW728_002015"
     CDS             6704..7708
                     /locus_tag="IW728_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1025359.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         2499 a         1390 c         1602 g         2305 t
ORIGIN      
        1 taattaaaac catgtgaaac tgcctcacgg cagcttacaa ttactttttc atcttcaccc
       61 gttaagtgat gaaaaaagaa cccctttctg ctatggttaa agtgactaaa ccaaccaaaa
      121 gaaaggagtt cttttcatga ctagcttaca caaaaaccaa gtaaaattca attcaaatat
      181 cactatttct catacaggtg gtcaattatc gagtgattcg ggtctcgtcc tagtgaaaga
      241 gctgatgaac atctttggtt tttctgaact ggctaagcaa cacattcaca ttgaagacga
      301 acgagcgtat tttactcatg acaacttatc catacttgag cagttcatta tgcaattaat
      361 tgctggttac tcagctgatt ctgcagctaa tctcctgaga caagatcctg tgtttaaagc
      421 tgttttagat aggaaagaac tcgcttctca atcttcactt tcccgatttt tagatcgact
      481 atctgaggag aatatccatg aacttcaagc tttgaaccaa gaacttatcg ataaagcacg
      541 cctcatccgt aatgatacgg aattaatcat tgatttagat tcgacccatt cagatacatt
      601 tggtcaccaa gaacaaacga attataatac acactaccaa acctatggtt accatccatt
      661 ggtagctttt gacggattga ctggtgactt cttaaaagct gaactacgtt caggaaatca
      721 atatacatca aaaggcgtaa aggagtttct cacaccttta ttagagcact ataatcactc
      781 tctaccaaac actgacatct tggttcgtgg agacagcggg ttcgctacac ctggtgtgta
      841 tgattcgtgt gaatcaaaaa agagtcatta tgttattcga ctgaagaata atcgtagact
      901 aggtcaaata gctgagaaat cagtacttta tggcgataat caaaagtggg aagaacgaga
      961 agttcagtac ttctcgacca cttatcaagc acaatcctgg tcacaaagtc gtcgcgtgtg
     1021 tatacgctca acacgtgaag cgggcgaact actctttcga catgaattta tcgtgacgaa
     1081 tctatcagaa aatgtttctc ctgataccat cttttctatc tatgccaaac gtggcacaat
     1141 ggagaacttc attaaagaag cgaaagctgg cttttacttt gacaaaacag acagtcctcg
     1201 ctttttggag aatcatgtcc gaatgatgct cagcttgata gcttacaact tagtcaactt
     1261 cttaagaacg attggctttg aagaagtcca aaagggaatg accattcatt ctattcgatt
     1321 gaagtttctg aaagttgctg ggaaattagt ccaaacgggt agacgagtct atctcaaatt
     1381 atctagttat catgtgtatc agaatgaatt ctacagggtc tttgctcgcc tgaggcgagc
     1441 cagtcaatgg atctaatcca acaatctaac gattttttta ctgggaagtt atccagggag
     1501 aagtatgctc aaaatgcctt acacccaaaa tttattcctc aatatttttg aacagtgaat
     1561 gtttgaagta actttttagt caaaatcagt tgatagggat attagtgata catatttcaa
     1621 taaaatgtgc caaagaatta aagctatgaa ttattcaggt atttaaggat tgttaaaatt
     1681 taatcttaga aaaaaataac tggaacaaga gctatttgtt tttacagcaa agaatggtat
     1741 atttttgtag gaggtggctt acttgaaaga agtcgttatg atagatgctg caagaacgcc
     1801 gataggaaag tatcgcggaa gtttaagtcc atttactgca gttgaattag gcacacttgt
     1861 gactaaagga ttattagata aaacaaaact gaaaaaagat aaaatcgatc aagtaatatt
     1921 tggaaatgtc ctgcaagctg gtaatggtca aaatgttgcc agacagattg ctttgaacag
     1981 cggtctccct gtagatgtcc cagcaatgac gatcaatgaa gtttgcggat ctggtatgaa
     2041 agcagtgatc ttagcacgcc agctgatcca attaggtgaa gcagaacttg tgatagccgg
     2101 cggaacagag agtatgtctc aagcacctat gctgaaacag tatcagtcag aaacaaatga
     2161 atatggtgaa ccaatttcca gtatggtcaa cgacggattg actgacgcat tttcaaatgc
     2221 acatatggga ttaaccgcag agaatgttgc aacacaattt tctgtgagca gagaagagca
     2281 ggatcgctat gccttgtcgt cccagttgaa agcagcacat gctgtcgaag ccggtgtatt
     2341 ttctgaggag atcatcccag tcaagatttc tgatgaagac gtgttatctg aggatgaagc
     2401 agttcgtgga aatagtacat tggaaaaact gggcacgtta cgtacagtat tctcagaaga
     2461 aggaactgta acagcaggaa atgcttcccc gttgaatgac ggtgcctctg tggtgatcct
     2521 tgcatccaaa gaatacgcag aaaataataa tctgccttat ttagcaacca tcaaagaagt
     2581 agcggaggtc ggtattgacc caagtatcat gggaatcgct ccaatcaaag ccattcaaaa
     2641 attgacagat agatcaggaa tgaatttatc aacaatcgat ctttttgaga tcaatgaagc
     2701 atttgcagct tcttctatcg tagttagcca agaactgcag ttagatgaag aaaaggtcaa
     2761 tatatatgga ggagcaatcg cattaggcca tccaattggt gcaagcggtg cacgtatctt
     2821 gactacatta gcttacggat tgcttcgtga acagaagaga tacggtattg cttcattatg
     2881 tattggtgga ggtttaggct tggcagtctt gttagaagcg aatatggagc aaacacataa
     2941 ggatgtgcaa aaaaaaaagt tttatcagct tacgccatct gaacggcgca gtcaattaat
     3001 agaaaaaaat gtattgacac aagaaactgc gttgattttt caggaacaaa cactttctga
     3061 agagctctcc gatcacatga tcgaaaacca agtgagtgaa gtggaaatcc caatggggat
     3121 cgcgcagaat tttcaaatca atggcaagaa aaaatggatt ccgatggcaa cagaggaacc
     3181 atctgtcatt gcagctgcaa gtaatggtgc aaaaatctgt ggaaatatct gcgcagaaac
     3241 acctcagcgg ctgatgcgag ggcaaatcgt tctatcagga aaatcggagt atcaagcagt
     3301 gatcaatgca gttaatcatc gaaaagaaga gctgattctt tgtgcaaatg aaagttatcc
     3361 ttcaatcgtg aaacgcgggg gcggtgttca agatatctct actcgggaat tcatggggag
     3421 tttccatgca tacttatcta ttgatttttt agtggatgta aaagatgcaa tgggcgctaa
     3481 catgatcaac tcgattcttg agagtgtggc aaacaagtta cgggagtggt tccctgaaga
     3541 agagattcta ttcagtattt taagtaattt tgctacagaa tcattggctt ctgcctgttg
     3601 tgaaatccca ttcgaacgct tagggagaaa caaagaaatc ggtgaacaaa ttgccaaaaa
     3661 aatccaacag gcaggggaat atgcgaagct tgacccttac cgtgcagcaa cgcacaacaa
     3721 agggatcatg aatggtatag aagctgtagt ggcagctaca ggaaatgata cacgtgcggt
     3781 tagcgcttcg atccacgcct acgcggcaag aaatggatta taccaaggat taacagactg
     3841 gcagatcaaa ggggataaac tagttggaaa actaacggtg cctttggctg ttgccacagt
     3901 aggcggagct tccaatatcc tgccaaaagc aaaagcatct ttagctatgt tagatattga
     3961 ttcagccaag gaactagcac aagtaattgc tgcagtaggg ttagctcaaa acctggctgc
     4021 tttgcgtgca ttagttacag aaggaatcca aaaaggccac atgggacttc aagctcgttc
     4081 attagcgatc tcaattgggg ctattggtga ggaaatcgag caagttgcga aaaaattacg
     4141 tgaagcagaa aagatgaacc agcagacagc tatacaaata ttggaaaaaa tccgggaaaa
     4201 ataaaaaagc aatatcttta gaagaataaa gcaagattta cagttatttt atttactgca
     4261 gatcttgctt ttttctctat cagaaaatat gtttattatt agaatttttt atagttaagg
     4321 ttaggcatct taagaaatca ggcaactcca agatatcttc aggtagaaga gaagtcgcct
     4381 aacattgtag gatcataggt gatttgttgg acaggtcaag ctgatatttg agcctgatgt
     4441 tagaaggaat ttctttttac ttgctgtaga aattattata tgactaaaaa ggctgggatt
     4501 ttaaatccca gccttttatg acaaatagaa tttttatcga tcagtaatcg ctgtcgctgc
     4561 aaattgttcg atcaaaggaa tcaaagtcaa tttgactttg tatttccgtt cgagtgcttt
     4621 ttcgataacg aactcatttt cagtagtcat agaaatagtt gctaatttac gaggttcatc
     4681 agcatttttt tcattaataa ctacttcatg ttcattttgt tctgtgtaaa atttaggata
     4741 ctcagcaacg gcttttgaaa aatcagcaaa acttaaagcg gcattcgtat cagttgagcg
     4801 tgtattttca gttgtgtccg cttttggttt ttccgttttt tcttctggca cactgactgt
     4861 ttcttctgta actggtgtcg caacagcttg cgcagttggt tcatcgtttt tcaatcgttg
     4921 atcattccag taagacacga tttcaataag aagattgtca cggaatcgtt cgaaaatccc
     4981 gttttctcct gaacgaattc tctttaatac agtattgaag ttgtcatcga ttggcacgtt
     5041 gatccgtgta tcttcatttg ttttcccagc taatccgatc gttaagattt gacggttttt
     5101 gggatcaagc atgacttgta acggcaaacc ataacgtcgc aagctctctt cttctagaga
     5161 acggaataat tgtcctctta caccatccaa tgaaggacgg aattgattta ataatttttg
     5221 tttctgtttt ttcttcatat gttctctcct tcgctcatac cctttcgatt ataagaaaaa
     5281 aacagccaaa tgaaaaggag taatgcaaaa aaacttgcgt atacgcaaat tacaacagaa
     5341 aaatttgcgt attttcccgt tttttgaaag aaaaataaaa aatggaacgg attatgtata
     5401 tatacataat ccgttccatt ctttctattt tttcggtatc aaggcaaaaa caggtagttc
     5461 aggtgaataa atgacttcat gttcgtgcca gggtttgacc attagcgaat gatcgaaccg
     5521 ttttttcagt tcttcgatga tttgatcatg tgtccagttt cttgcaggaa aatgttggat
     5581 tttctctgca tcgatcaaaa agacaaaatc ggaatgaaaa gtctgggtaa cttcttccga
     5641 gagagaattc aagcgttcga ttggatccat aaaatcacct ttcgacttaa aattgggtag
     5701 tatctgggtc ttttgctttg ccgactacac catctaactg atcgatttgt ttcatttctt
     5761 cttctgttaa ttcaaaatca aataattcag tattttcttt gattctgctt ggtgtcactg
     5821 acttaggaag aggaaggaat tcgtgttgca gcgaccaacg caaagcaact tgtgcaatcg
     5881 tcttgtcatg tgcttcagcg atttgtttca tttcaggcac atcgaagatt ttaccagtgc
     5941 ctaatggact atatgcttcg aggatcatat catgtttttt tgcatactca actacagttg
     6001 tctgtaattc gcctggtgca aggaaaactt gattgaccat cggcataatt tttgctgttt
     6061 ttgctagtgt atctagatgg tggggtaaga aattgctcac accgattgct ttgattttac
     6121 ctgcttcata cagttcttca aatgctcgcc atgtatctgc atttgcttgt tcccaattat
     6181 cacgaaaagc aacagggttt ggccagtgga tcaaaaacag atctaaataa tctgtttgga
     6241 gcttcttcaa tgattcttca aaagaagaca tcacaagttc atagctgtgg tttgcattcc
     6301 ataattttgt tgtcaagaat atctcttctc taggaattcc actttcctta atcgcttggc
     6361 cgacgctttc ttcattcttg tatccttggg cagtatcgat atggcgataa cctgcagcca
     6421 gcgcttcttt aacagaggaa acagcgacat ccccatcagg tgtttgccat gtgccgaatc
     6481 ctactaccgg gatttcgtaa ccatttgata gacgataagt gcttgttaat gaattcatac
     6541 tttcagctcc ttttttattc gttcaactat aagtgtagcg catttttcga aaaaaagaat
     6601 ccattttgtt tctactcttg aaatttttgt ttaaaagaaa atgattttga ttctggtatt
     6661 tttgattttt tttccgtaaa ctaagacaga agggaatgag aaaatggata tctatttaaa
     6721 aaaagcaatt ctgcatatta ttgacagaga aactggttct ccagtctttt cacaaaaaga
     6781 gctggatctg acaaaagaat atattcgtga ttttcttcaa aagaagatcc aaaaaatctc
     6841 ttctgctcaa acaaaaactg gacaactagc agaagaaagc atgatcgttg aatcacttaa
     6901 aatcgctcaa gaaaattttg tggaagcaag tgaagcaatc gttcagcggt ggttcgatat
     6961 ctaccaagaa agtgaagaag cacctagtgc agatgtattt gttgtcttgt atgaattaga
     7021 tacagtgatg tacttatctt tattgaaagt caattatcgt gaggcttaca ctcactttgt
     7081 tgaagcagac gaagcaggga tcgacaaccg cttgattttg aatcgagcaa ttctaggggc
     7141 gaaaacacaa aaagcagatg aagcattcgc tgtgaatttg actgattgga cctacgaatt
     7201 aattgaaaaa aaatatgagt tttctggcaa aaaagagctt tatttttctg gaagagtgat
     7261 cgaaagtgtt cctgcacctt ctttagaaga aaatgtaaaa gtgatccaaa aagtagccaa
     7321 gcatcttgga aaaaaatttg agacagaaga atttgatatc atggctgatt tgaaagaagc
     7381 tgtctatgat acgatagaag aaaaaggtcg gttggatcat gagatgattg cggaaaaagt
     7441 cttcaaggaa aacatcacgg caaaaatggc ttttaaagaa gaagtacaag agcaaggatt
     7501 tgtcccagaa gcacctcctg taaaagaagt acaagaaatt tctgagaaaa aatttggaaa
     7561 acaaaaattc aagctatcaa acgggatcga actgatcgta ccagtagatg tatatcgcaa
     7621 cccagatttg attgaatttg tgaataatcc ggatggtacg atatccgtga tgatcaaaaa
     7681 tgtggatgaa gtattgaatc gtttataaaa atgactagaa tattaggtgt tcaagtacca
     7741 gtgatcagtt aaatgaattg tgtcaagaga aggatcggaa aagacgaata taacaa
//
LOCUS       DACEMO010000126         7698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEMO010000126 DACEMO010000000
VERSION     DACEMO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.131"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            543..1106
                     /locus_tag="IW728_002016"
     CDS             543..1106
                     /locus_tag="IW728_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1025360.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1385..1672)
                     /locus_tag="IW728_002017"
     CDS             complement(1385..1672)
                     /locus_tag="IW728_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1025361.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1662..1991)
                     /locus_tag="IW728_002018"
     CDS             complement(1662..1991)
                     /locus_tag="IW728_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1025362.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2296..2625
                     /locus_tag="IW728_002019"
     CDS             2296..2625
                     /locus_tag="IW728_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025363.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2912..3397)
                     /gene="vanZ"
                     /locus_tag="IW728_002020"
     CDS             complement(2912..3397)
                     /gene="vanZ"
                     /locus_tag="IW728_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1025364.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3550..4461)
                     /gene="vanY"
                     /locus_tag="IW728_002021"
     CDS             complement(3550..4461)
                     /gene="vanY"
                     /locus_tag="IW728_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ1025365.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4889..5497)
                     /locus_tag="IW728_002022"
     CDS             complement(4889..5497)
                     /locus_tag="IW728_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1025366.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5503..6534)
                     /gene="vanA"
                     /locus_tag="IW728_002023"
     CDS             complement(5503..6534)
                     /gene="vanA"
                     /locus_tag="IW728_002023"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1025367.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6527..7495)
                     /locus_tag="IW728_002024"
     CDS             complement(6527..7495)
                     /locus_tag="IW728_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1025368.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2352 a         1532 c         1280 g         2534 t
ORIGIN      
        1 taaaactttt ttagcagatt ttaaagtttc gtcaaaacta atatattctg gtagctgagc
       61 attcaaatag ctttcagact tcagaagaaa attacgagca tttttatagt ccatctctaa
      121 taaagttatt ctttttttat ccaatacatt cagctcaatt ccttataata ttctattcct
      181 tagtatctca acattattta ctttataaaa cgaataaatt ttgtagttct aattcaaata
      241 ttgcatgtta tatctaatat gggagcattt tataattttc attgtgataa actcgttatt
      301 aactttagga tacactagtt aggattaaat taggtatcta gaaatttact taagattttt
      361 ttgtttattt cgtatacaca ctcgtatcaa aaacaatcga ttatgagaca aaatgaaatg
      421 acctctttta ttaaataaaa gaggtcatat tttgtatcat aacttgttgc attaactatg
      481 tatctataac tcccttttat gatacaatca agccagaatt aaagtacgaa aggaagaaaa
      541 gcatgagtgg atacatttat ggttatgccc gagtgagtac acgttcacaa gaactaaatc
      601 gtcaattaga cttactcaat gaacaaaatt gtaatgaaat cctaactgag aaaatgacgg
      661 gaaccaaagc ggttcgtccc caactaaatc gtttaaaaga taaaattcgt ccgggagata
      721 cggtaattgt agaaagtttt tctcgattgg gtcgtagtac aaaggatttg attgaccttg
      781 ttacctattt tgaagaacac catgttaagc tggtgagctt gaaagaaaac ttcgatacca
      841 gcacgcctca cggacgctta atgatgactg tcttccaagc atttagtcag ttcgaacgtg
      901 atttaattgt tgaacgtacc aaagaaggtt tgaaaagtgc acgtgcgaga ggtcgtaaag
      961 gtggccgtcc acgagtgaac cagaaagatg ttgatcgagc agtaaaattg tataaaagtc
     1021 aggtttatag cgtaaaagaa attacggaaa tgactggtat tagcaaggca acactctacc
     1081 gctacttaaa agacaatagg gaataatcta ttttaatctt taggaatcta taatatttat
     1141 gggagaatta gagtgatttt tttattatta ctaaaaaaca atataaaata acgcatttta
     1201 agccctcttt gtgcataagg atgaagcttt taatttgaaa gaggcttaaa atgcgtttta
     1261 attttttcta tttttcataa atatataggt tagtggttta aaattatagg gtattacaac
     1321 tctcagctgt taaaaattat cttttaaagt gtcgtctatc ctctcaagtt gaaacattgg
     1381 gttcttacct aatctttttt aattgttcat aaaaattttc atgcgttcca catagtaaga
     1441 taggtatcac tgcattatct ttaatttcat atgcaacgcg atatgtcgta cctgcatact
     1501 tgaatccaat agcccaaatt cctgataaat cacccgtttt ttgttctccc gaatgaggac
     1561 gaactgctat ctcatcataa attatcgtta aaaattgttc ttttaatgtt ttctctttta
     1621 atttttttag atactttatt acgtgtttag aaggtagaat ctcataagcc aatttctgct
     1681 gccaactcct cttttgtcat cggtttagaa tttgcaacag tatcttcagc aatagattta
     1741 aatgcagaag ttaacgcatt ttttctattt ctaaattctt ttaagatctc ttgtttgcta
     1801 taaccttctg aaagaatatc agccaaaata tcttcactaa aatcaaaaaa ttctttttta
     1861 ggctctacaa actcataaag gataccattc tctacaagct tagcttctac ttcaacacca
     1921 ttgggcacat cgaattcttt agggaccgtt aacataatag aatttccttg ttttcttgtt
     1981 tttgtaatca tattatttcc tccattattt tacatttcgt aattacttag taattacatt
     2041 gtacgctatt tttagtagaa atacaaaata ataaaactcc tttagaacaa ttttcaaagg
     2101 agttttattt gattaggaaa ttatgtagaa taataatatg tttttaactt ggaatcaaaa
     2161 acgcacttgc aagtaagttt acttcaatac atgaaattgc ttataatagt gtattggaat
     2221 taaggtagat tgacaaaaga gtgatcaaat aggtatactt cattcaaata atcgtttgaa
     2281 tgtgaggtga gatgaatgaa aaaagtttgc caagtgactg tcgtacataa agaaaaagta
     2341 aatggaataa aaaataaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
     2401 tgtttcagcg atttttctag aattaaaatt ttctatgctt tagaaaccta taaggaaatg
     2461 tgcgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
     2521 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattac
     2581 tctttagcga atgaagacat cctttccgct attcgtgttt tttaaagtta tcagaaaatc
     2641 tatctatgaa atcttagttt gggggtaccg tcaggaaaat gcggatttac aacgttaagg
     2701 aaatttatta aagagaccaa ttcctcagca ctaaaattga taattgggct ttctttgtta
     2761 ctatagtgtt aggtaagctt tttactttat caccggtttt cccctcactt cacacctacg
     2821 ggcgagtttc cccacatccc gttttccatc taatccaatt cattcaatat ctttatgtgt
     2881 atgaaagatc agattgcttg atttagacat cttacacgta atttattctt aaatgggtac
     2941 ggtaaacgag caataatacg agcaaaagta tacctacaaa aataataact ctgtctaatt
     3001 ttttttgatt catatgctta ttgcttaaac catataattt cagtccaaga aagcctccaa
     3061 cagtatttgt aattacatct gttatgtctg tcgctccaat agcgaagata aattgaatta
     3121 tttcaaaagt aagacttaaa accagtacaa aagcaaactt aggtaaaaat ccgatttctt
     3181 taaaattgac attcaaaagc aagccaaatg gaataaagat tataacatta tctatcatct
     3241 ctctgaaatt cccagtagca gtaaatggag tcaagttaag acttctttga tgataattaa
     3301 atactgataa aatattgtat tgtaatttga ataacactaa ccagattagt gtcactaaat
     3361 ataaagctag caatcctcta gataatattt ttcccaatat aatttcctct aattcttttg
     3421 tttaagttgt atcattagta tagcaatgtc ctagttaacg tgggttttaa attaaaaagc
     3481 taacgttacc ccaaaagtgc tagtacaata atgctgttca ttaatttcgt tggtttcatt
     3541 ccgccatcct tacctccttg aattagtatg tgttgatccg ggaaacactg tcacaattac
     3601 accgtctata ttgtttcctg atatctcata acgaagatta gtcggcacat gaatggtggt
     3661 atttttagta acaggataat aaaagatctc atatttttcc ccatttacac taacagaaat
     3721 ggttttttct tcttttaggt aatccatata ttcctcgaga acgaaattct tttctttcat
     3781 aatcgcacta tgtggtaaac caacatagcg aatatgccat agttcatatt gaattcctgt
     3841 taactctgtt ttgtcctctg gataacgtaa aatgaacccg tatttccaag cattttcttc
     3901 tatccacttt ccttcagggg ctcgttccat tttcgtcaag cttgatccta catctagtga
     3961 taaacctgaa ttatgctcac tataacctgc tggtaaggca tactcagccc ccatttcttg
     4021 gtaaagcaca ctttgctcat caaagtctcg atagccacta ttaataataa aatgactaac
     4081 gccacccttt acagcatcat tgaccatctc tgaaaatttt tgtgctattt cttttgacat
     4141 ataaatatta ctatcaagca acccgtatcc atttattaat tcgtcatgtt tagataaatt
     4201 cacgatatct gacttcacac tttcttggcg aacaggatat ttactattga ttaatagcag
     4261 atttccttga taaacctgtt cttctgtaat tgttttctct tgggtatttt cagaagtccc
     4321 actattttct aaatgttctt tgggattttg atcataattt tgaaattcga ctttttcctg
     4381 agaaaacagt gcttcattaa cgtagtcata acctaagtat attaagaata acaataacaa
     4441 taaaaaaaac aacttcttca ttttcagtct cctccttaac tatatagttg gaagtatgaa
     4501 atatttctag aatcttcccc ttaaacggca caatctcgtt tgagattgtg ccgttttgcc
     4561 ccgtaaatta cagtcaatta tttcgcccta ctggatttac tattatcacc aatgtagatg
     4621 aattttcata agtgataccc atgctgaatt taacgaggct gaactgcctg ttgacaatgg
     4681 gcggtgtgaa ataccacccc tctatgcgat cagtcttatt atcaggctat cttgccgcag
     4741 cgcacgcaga taatgaccgc aatatcgctc tgccttacat cttcaatact atctactata
     4801 tataaattcc ctaagaacca gttacatacg tcgggtttcc tgccgaaaga gttagcttat
     4861 atagtttgtc cgtgcaacgg ttaaaagttt atttaacggg gaaatcaaaa tagctattgg
     4921 ggtatggttc gtctcttaat acatagtgcc accattcgag gctatatgct tcaaacccac
     4981 tgttttccat gatggagcgc aaacgtctgc gattttgcgc ttcattgcat gatattccat
     5041 ttgccgcatg atgagagcgt tcatccataa aatcaaatcg gctccccatt ggtacaagct
     5101 cacccgtgtc taatcgataa agcgtaagat caatggcact gccgcggcta tggcttgatt
     5161 ttgaagccac gtatcctttt gaaatcatct cagttcggtc aatattggga taataacttt
     5221 cctttgtcag gttattttcc ggctgtgcag cccattgcat aaaacagttt acagcacgat
     5281 taggacggta accgtcccat agaagcaatc cgtacccttg ggtagcagcc agttcttttg
     5341 ccttcaaaag cgattcagcc aactcgtatg tccctacaat gcgatttact tcataaccgt
     5401 caaccggttt tccggtgaaa ttatcccaag tggcatattt agcgtcccaa cgaacaccgt
     5461 gtactatttc atctaaaaaa gtaaatccta tttccatgct tatcacccct ttaacgctaa
     5521 tacgatcaag cggtcaatca gttcgggaag tgcaatacct gcagcggcca tcatacgggg
     5581 ataacgactg tatgacgtga aaccgggcag agtattgact tcgttcagta caatgcggcc
     5641 gttatcttgt aaaaacatat ccacacgggc tagacctcta cagccgagcg ctttatatat
     5701 tttttttgcc gtttcctgta tccgtcctcg ctcctctgct gaaaggtctg cgggaacggt
     5761 tataactgcg ttttcagagc ctttttccgg ctcgacttcc tgatgaatac gaaagattcc
     5821 gtactgcagc ctgatttggt ccacctcgcc aacagctaac gcggcactgt ttcccaatac
     5881 cgcacaaccg acctcacagc ccgaaacagc ctgctcaatt aagattttgc tgtcatattg
     5941 tcttgccgat tcaattgcgt agtccaattc gtccgcgcta ttgacttttt tcacaccgaa
     6001 ggatgagcct gaacgcgccg gcttaacaaa aacaggatag gtaaacgtag ctgccaccgg
     6061 cctatcatct ttattaataa cccaaaaggc gggagtagct atcccagcat ttttcgcaac
     6121 gatgtatgtc aacgatttgt ccatacaaat tgctgagctt tgaatatcgc agcctacaaa
     6181 agggataccg gacaattcaa acagaccttg tatggatcca tcttcacctg acttgccatg
     6241 caaagctgaa aatgctacat caacatggtt gatttcatat tcatggttct ttttaacaag
     6301 taatccgtgc atttttttat ccggcgagag tacagctgaa tagcaattgt cgttttccca
     6361 ttccgcgcaa ggtttttcgc acattttcca tacaccagat ttcgtaattc caatgtataa
     6421 cggctcgtat ttttctttat taatgttagc ggctatctct attgcagatt ttaccgatac
     6481 gtcatgctcc tctgagcaac ccccaaacag tattgcaact tttattctat tcatgctcct
     6541 gtctcctttc aaaatccaaa cagtttttaa tggttttttc aacggtatca cgcaacgctt
     6601 gctcggtata ataggccgta tgcggtgtga ttatcacgtt aggcattctt tgaagtttaa
     6661 gtaaaaattg attatcaatt ggtttttggg tgcaatcaga gtagaaaaac tcttcctctc
     6721 cttccaatac atccaatgcg gcaccgccca gtttcccgtt ttctaatgct ttaaccaact
     6781 cataggtatc tacaagtgga ccgcgcccag tattgataag aaatgctcct tgcttcattc
     6841 tctgtatttg ttcgtggctg ataatatagt gcgtatccgt attgagcggc acatgaagcg
     6901 taacgatatc gctattttgc agcaactcat caaacggtac atagtttacc tctatacttc
     6961 ggctgcgact ataagccaac actttacatc caaatcctcg cagccgctca ataaccgctt
     7021 tgcctatctg gcccgttccc accacaccaa ctgtcatgtc gctgagtacc ttgccacggt
     7081 cgctgtccaa cctgaaatca tgtttttcca cagagcgcac aatcgatttt acgttgcgta
     7141 ctgccataag aattagcatc atagtataat cggcaacgct atccggcgag tacgccacat
     7201 tgtcgacagt gatgcccatt ctcttagcag cagttgtatc tatatgattg cagccgatgc
     7261 ttcgggtaga aatatatttc acaccggctc tcttcagcgc aagaagaata gaggcggaaa
     7321 tctctgattt atgtcccaca ctgatacatt gattgaaagg cgcggatttg gcgttggatt
     7381 ccgacacgtt ggcgttaatt atcgttgcca taacgccaaa gcgaggcgaa agagcatgga
     7441 atgcatctgc ctcatcctgc tcacatccat aaacagtaat gccgatgtta ttcataatta
     7501 agaccaaccc tttctgtgaa aggcaccaaa taaaacgggg atgccaatgg taatcttgtc
     7561 aaatctttct ctgaagaacg aaaacggctc gttcttacgg tattaagtct accgtgtaag
     7621 aacgagccgt ttcttaatat taaattgtta atttcctaag aaaaagtaaa gataaccttg
     7681 agattttcct aaaaaatt
//
LOCUS       DACEMO010000127         7696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEMO010000127 DACEMO010000000
VERSION     DACEMO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.132"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1371)
                     /locus_tag="IW728_002025"
     CDS             complement(<1..1371)
                     /locus_tag="IW728_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1025369.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKL"
     gene            complement(1373..2041)
                     /locus_tag="IW728_002026"
     CDS             complement(1373..2041)
                     /locus_tag="IW728_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1025370.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2172..2756)
                     /locus_tag="IW728_002027"
     CDS             complement(2172..2756)
                     /locus_tag="IW728_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1025371.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3087..3320)
                     /locus_tag="IW728_002028"
     CDS             complement(3087..3320)
                     /locus_tag="IW728_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1025372.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3335..4285)
                     /locus_tag="IW728_002029"
     CDS             complement(3335..4285)
                     /locus_tag="IW728_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1025373.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4282..5124)
                     /locus_tag="IW728_002030"
     CDS             complement(4282..5124)
                     /locus_tag="IW728_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1025374.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            5446..6633
                     /locus_tag="IW728_002031"
     CDS             5446..6633
                     /locus_tag="IW728_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1025375.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            complement(6725..7465)
                     /locus_tag="IW728_002032"
     CDS             complement(6725..7465)
                     /locus_tag="IW728_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025376.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
BASE COUNT         2526 a         1389 c         1074 g         2707 t
ORIGIN      
        1 aagttttata tcttttttta ttccaccatt ttttaaataa ataattctat tgcatgtttt
       61 ataaagaaat tccggatcat gagtggctat aactactgca cccttttctt ttaagtcttt
      121 aataaggtca gcaatccttt ccatattgat ataatccaac ccacttgtag gttcatcaaa
      181 tagaaataaa ttagtttttg acaatgaagc tatagaaacc aataatcttt gtttttctcc
      241 accggataaa tcaaacggta attgatcttt cattttataa agttccattt tttttaaaat
      301 tttttcttgc tgatctttac taactatttc attccccagg gctaactcgc tcctcactga
      361 ttctgtgaaa aattggtaat ccatttcttg cattactaaa aacggcgatg acaaattttt
      421 tttattttcg attatctttc ctttggtagg gcgaataatc ttcatgagta ttcttagtaa
      481 ggttgttttc cccacaccgt ttttccctac caaaccaata acatctccaa ctttgacatc
      541 aaaatttatg tcttttagaa tattttcata agtaatttct tcaactttta atacatctgc
      601 acttaccttt tcgatatagg gaacttcaat cgaactatta aaaatgtcaa atttccttaa
      661 tgcgttattg ttttcagttt ttaacaactg aatagttgta gtttttacag ttctattttt
      721 aacaaataga acttgatcaa ttaattcttt caaatagtaa aaacgatgtt cagctataac
      781 aatagtaaat cctttatttt ttaaatcaca aagtaagtca gcaagtttct tagtcgcttt
      841 gaagtccaaa tttgaagacg gttcatccaa tataattaat ttagagttta gcgataaact
      901 acatccaata gaaattttct gccgttcacc acttgatagc gaaaaaatgt ttttatctaa
      961 tatatttgaa agatctaata attcagctaa ttcattgatt cttttatcca tatgctcttt
     1021 tgtaaatcca aagttttcca tcgaaaatac taactcactc gttgtatttg ttgtaaagaa
     1081 ttgtcctcta ggattttgac ttacgtagcc aatatttttt gatatacttt cttgtgttaa
     1141 ggattgtagc gagttatttt caaataaaat ttccccaacc aattttccgt cataaagtct
     1201 tggaataagt ccattcatta actttaatag tgtggatttc ccactcccag aagaaccagt
     1261 aatcaatgta agactgcctt tttttattga aaaattaagg tcaacaagcg cttgttcctt
     1321 aacatcctta tatgaaaaag aaacattgtt aaattcaaac aattctttca cctcaaatca
     1381 aaatacttaa gaaaaaaata gtaacggaaa acaggatgat atagtctaat aagctcatgt
     1441 ttgttttata gtaagaactt cttttcacag gagattctat cccctttgtt attgcattag
     1501 cggtcatttc ttcagcaatc atagttgcac gtataagaac gggaactaac agaaattcca
     1561 aatattgcag aggctgaaaa aaagacaagc gaatgtttct catttttaaa gattctttaa
     1621 tcattcttat ttcagaacga taagtcggaa agaatctgat cactactgta atacttaaca
     1681 ttattttttt cggtaaatgt attttttcta aagaggataa taattcattg ctagtataga
     1741 cactaaccaa caaccatgag atcattacga ccggcaacat tcgaaataca atatataagg
     1801 atgtcccaaa aaatatagta aaaaactcta tcttgatagc atcaataatt ttcattacta
     1861 aaaacagaac tgaataaatc aagatgaaat aaatacatgt ctttatttta cgaaacatca
     1921 atactaaaac tgtagcaata atgaaacacc ccacttgaaa tatatttccc tttcctaaaa
     1981 aaataatgac cggaaaaaaa atatttaaag taatcaaggt taacggattg atttttgaca
     2041 ctctttcacc tccaaaaaat aagatcgcac tgttatctta agctgacaaa aatgcaccgt
     2101 tattcagata acagtgcgat cttatcaaaa agcataactg agaacaattc tcaatgcaag
     2161 ctttttttta attaactcag taattcaatc cacccaggaa cagaaaattg atacaatctt
     2221 ctggttaata gcatagtctt ttcttttgaa tagctatgct tcaatatatc atagaatgta
     2281 gcgtagaatg agtaaatgta gaagtggatt tcttcttcat taaagactaa attttgattg
     2341 atcattccga tagtctttaa cttatctaga caacagactg tcttttttgt cacaaatgat
     2401 gtaatttttt caacaatatc ttgatatttg gttccagctg attggaataa aaccaaatcg
     2461 aatatacgtt tattctcaaa aatataatta ataaatggat ctaattcatt gaagttctct
     2521 ctccaacttt tctctatatc tatattttct aagtttaaga ggtatttggt aaactcttca
     2581 ttatacatat taataagcgc attaatatgc ggctgaataa tttcttcaaa taacgcttcc
     2641 ttagaagtaa aatgtttata aaaagcaccc gtagttacgc ctgcggtcaa gcacagttgc
     2701 cttatcttag tttgctgaaa tccttctttt tcaaaatggc ttagtccact ttctattaat
     2761 ttttggtgag ttgtaatata atctctggtt tctatgggta cttcctccta ataagatgaa
     2821 ttaactatat tatcttacac aggacttgaa gtttcaatcg ttaacgtcta ttactgaact
     2881 cattccttag agctaaacta atgagaaaga gcaaaataac tgatacaaaa agcaatccta
     2941 caagaattaa tagactcatc taaatcattc accttttcta aaataatccg agaaagattt
     3001 aaaaaaatta tatccagcta ttagggtaag tcctatcata atcactccaa tagccatttt
     3061 cttatcctcc ctaaaaataa agtcttttac ttaaaacctt ttttgtaatc gtcaataaat
     3121 tttttccctt tatcagttaa ttgaaatcct atatctaaat agtcctcata tctaagttta
     3181 gccagcatct cacttacaga tgctggacta acacttagaa cttttgctaa atcattattt
     3241 tttaatttct tttttctttt tgataattcc tgaattgcta gtaaataaat tagtatagta
     3301 gtcgttttaa tttgtaccat acctaccctc caaattattt cgaaaggcct tcagcaattt
     3361 tatccaggtt ccatttcatc atcgcatagt aactatcacc atcttgtcct ttttctgcaa
     3421 ttgaatcagt aaaaatcgtt gaatagatag gaatattggt atcttttgat actgttttca
     3481 ttggtctatc gtccacactg ctttctacga ataaggaggg aacttttgtt gtgcgtaatt
     3541 tttcaactaa gtgttttatt tgatctggtg ttccttcttc ttcagtgttg atttcccaaa
     3601 tgtaagcaga aggcacatta tacgctttcg agaaatattt aaagcatcct tcacttgtaa
     3661 caatcatttt tttatcattc ggaattgaag caaatttaga tttagcttct ttgtctagag
     3721 aatccaattt ttcaatatac ttatctagat tttctttata gaatttttta ttatcaggat
     3781 ctttttccgc taactgtttt tcaatatttt tagcgtaaat aataccgttt tctaaattta
     3841 accaagcatg gggatcttcc ttccctttct cactctgacc ctctaagtaa ataacatcta
     3901 tgccatcact tgctgcaaaa tagtctttgt tttcctcttt gttcgcattt tttactaatt
     3961 ttgtaaacca agcatttcct ccagtttcca agttaacacc gttataaaaa atcaaatctg
     4021 cttttgaagt tttttgaaca tcttcaggca aaggttcata ttcgtgggga tctttcccaa
     4081 taggtacgat actgtgtaaa tcaattttat cttttgctat attttcagta atatctgcta
     4141 aaatcgaatt agtaactact acctttaatt tttcgttcga agccactgta tctttgtctt
     4201 tactatttgt tgaacatgcc ccgaatataa aaagagcaat caaaatcccc agacttccca
     4261 gtactgcttt ccattttctc atcataactt tctctccttc tttcttaaaa atttttgttt
     4321 tggtgagatt agaaaactaa ttacaaataa gaatgccgct gttaaaacaa tacttgatcc
     4381 tgctgctatg ttaaaactat aaccaatgaa caaacctagc aatgatgagc acgctccgac
     4441 tgtcgccgaa ataaatatca tagtttttaa gctgtttgca tataaatatg ctgtagcagc
     4501 tggtgtaatt aacattgcaa caattaatat agttcccaca ctttgcatag ctgttactgc
     4561 tactaaagtt aatagaatca ttaatagata atgataaaaa ctgacattca tccccattgc
     4621 ttttgccatt aatggatcaa aagaagtgat taataattct ttaaaaaata gaacaactgt
     4681 aaccaacact aaaacagaaa cgccaattgt aatccactta tcaatatctt gtacagctaa
     4741 aatatttcca aataagatat gaaataaatc cgttgagcta tttgcaactc ctataaggat
     4801 aactcctaat gctaaaaaag aactgaaagt aataccaatt gctgtatctc cttttattac
     4861 actattgctt tttataaaag taataatgat agaagctaat agaccaaata caatcgcacc
     4921 aataaaaaaa ttaataccta aaatataaga aattgctact cctggcaaaa cagcatgaga
     4981 aatagcatca cccataagag acattcctct taaaataata aaacaaccta ctgttccaga
     5041 aacaatccct ataactacag atgtaattag agcattctgt aaaaagtgaa agtcatacag
     5101 accattaata aaattttgaa gcatattatt cccccttgta tattagagtt agggttttaa
     5161 ccttccggtt ttgattggat aaccctatga accatcgtta ttttaatttg gtattaactc
     5221 atttcttttg agataatata tttattggat tgagaggcta agccgttccc cccttgggtc
     5281 attgaacccg cactcaagag actggctcct ctctctgata agtggattgc gtatgagtta
     5341 gatgttgaaa gtccttactt tactccgtat ctcgtacaag gtagtgacgc ttttcagttg
     5401 agtggatttc aatccaaaat tctttttttg aaaggtcaga tatacatgta ctttttaggc
     5461 attgatatcg gtaaacgcac tcacgttgct tctatcatga acgaggaagg aaaggtatta
     5521 ctaaaaggtt tttcatttcc taacaccact gaaggagcag agtcattaat tgagcgaatg
     5581 gtcgactatt ccggagcacc cagtgacttt gttataggga tggaagccac ggggcactat
     5641 tggctgtcca tcttttcata ccttcacgaa tcagattatt tgattcacgt tgtgaatccc
     5701 cttcaaacag acggatggcg taaaggtaca gaaatacgga aacgaaaaaa cgatattatt
     5761 gattctgttt tgattgctga cttaatgcgc tatggctcat ttgtcgaaac ggtcttatcc
     5821 gatgaaaatg ttttttcttt gaaacaactt tctcgttacc gaacttattt agtaggtaca
     5881 gcgagcgact tcaaaagaaa aattatagcc gtattggatc aagtctttcc agaatacgcg
     5941 acgattttta ccaagcaggg cgttttcgga aaagcttcta aagagttatt gctggcgttt
     6001 agtgccccga tcgacttaga aaacatttcc tcagaaactt tggcccaata cttagctgag
     6061 ttaagcagga accgaatcaa ggcagaaaaa gcggaccaat tgaagcaagc agcttctaac
     6121 tcctttggag taaaatttgc tcaagaagcg ttcaccttcc aactacgttc aatgattgaa
     6181 caattaaaat tcattgaaag ccaaataaag gaaacggaaa gagaaatcaa gcacataatg
     6241 gacactttaa attctgttat tctcacaatt cctggaattg gtcctataaa cggtgcgact
     6301 attcttggtg agatcggaga tattcaaaaa ttttctaacc cgaaaaaact cgttgcttac
     6361 gctgggatag acgcttccgt gacacaatca gggcaatatg aggcaacgca taatgttatg
     6421 agtaaacgag ggtctccata tttgcgaaaa gccttgtttt cggcggcttt agtagcttct
     6481 caatgtgatc ctgtttttag tgcgttttac cagaaaaaag tttccgaagg aaaacatcat
     6541 ctaactgcct taggtgcggt ttctcgtaag ctttgttacg tgattcatgc tattttgact
     6601 aaaaacgagc cttatataat tgtttctgat tagctattga acattcaaaa aaactaatgg
     6661 taagaagctt atttgttgta cccttttttc ggaattatct gttataaaat attttttgat
     6721 ttggctaatt tttacttgac tctatatagt tagtctctcc aataaatata gtatctccat
     6781 aggctttttt taaattctct tcattaaata cagattctac acttccgtga gcaattagat
     6841 ttcgattcaa aagaataata ttatcaaaat acttctttac cttgcttaaa tcatgatgca
     6901 caattagcac tgtttttccc tgatgtttca attcttttaa cgtatccata attattcgtt
     6961 cactaactaa gtctatccct acaaatggtt catctaaaaa tatgaaatcc gcattttgag
     7021 caagacatct tgctaaaagt acacgttgaa attgtcctcc agataattct cctatttgat
     7081 gattagaata ttttcccata tcgacctttt ccaaagcctt tgaaactctt tgccattctg
     7141 catttttaat tctttgaaaa actttcattc cggcatatgt tcccatagat acacattctt
     7201 ttacttttat tggaaaagta aaatcaatat cagctttttg ctctacatat gcaatcttca
     7261 ccagtttttt attgatatct tcattattaa tggagacctc tccctgatga gggataatat
     7321 ttaacattgc tttaagcaat gtagattttc cagcaccatt tggaccaata atacctgtta
     7381 aagcaccttc ttgtattttt aaagaaatct cgttcaatgc taaaaaatcg ttataacaaa
     7441 cattaagctt tttcacttct aacataactt tctccttcta aatgataatg attatcacga
     7501 tcatatggta tacaaaaatt atattttagt caaccctaaa ttttataatt tagatttttt
     7561 tctgttgctg aagattagaa tgacaaaact atttgaaact acgcgtatag taagtagctt
     7621 tcaaaatata taaaaaagga gcgttctcaa atgagaacat gctcctaaaa ctactttatt
     7681 ggcgtatgga aaaatt
//
LOCUS       DACEMO010000128         7554 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEMO010000128 DACEMO010000000
VERSION     DACEMO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.133"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(7..756)
                     /locus_tag="IW728_002033"
     CDS             complement(7..756)
                     /locus_tag="IW728_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1025377.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            1085..1465
                     /locus_tag="IW728_002034"
     CDS             1085..1465
                     /locus_tag="IW728_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1025378.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            1561..2070
                     /locus_tag="IW728_002035"
     CDS             1561..2070
                     /locus_tag="IW728_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025379.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            2147..2326
                     /locus_tag="IW728_002036"
     CDS             2147..2326
                     /locus_tag="IW728_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025380.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            2381..2944
                     /locus_tag="IW728_002037"
     CDS             2381..2944
                     /locus_tag="IW728_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1025381.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(2991..3506)
                     /locus_tag="IW728_002038"
     CDS             complement(2991..3506)
                     /locus_tag="IW728_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025382.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(3532..4401)
                     /locus_tag="IW728_002039"
     CDS             complement(3532..4401)
                     /locus_tag="IW728_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1025383.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4527..5207
                     /locus_tag="IW728_002040"
     CDS             4527..5207
                     /locus_tag="IW728_002040"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1025384.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            5360..6340
                     /gene="pta"
                     /locus_tag="IW728_002041"
     CDS             5360..6340
                     /gene="pta"
                     /locus_tag="IW728_002041"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1025385.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            6463..6936
                     /gene="tsaE"
                     /locus_tag="IW728_002042"
     CDS             6463..6936
                     /gene="tsaE"
                     /locus_tag="IW728_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1025386.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            6929..7453
                     /locus_tag="IW728_002043"
     CDS             6929..7453
                     /locus_tag="IW728_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1025387.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
BASE COUNT         2505 a         1339 c         1567 g         2143 t
ORIGIN      
        1 gtttttttat gcgtgttgtt gtgcgttgta gtattcttct tccatttttt gcatacgttc
       61 acgttctttt tggttatacc aaggtgaaac ggtaaatgca aggacatcat tcagcaagta
      121 aacagagctg ttcacgaaca tcgccaaact tgcagatcca tgcgtaaatg aaatatacca
      181 aaggaccatt tgagcaagcc ctgaagctaa ccaccagtaa tattggttgt tgtaacgcaa
      241 gaaactgatg ataccagcag tcaagctaat agaaaaagca atggcatcta cccacggccg
      301 aggatcatct gtataacgac caatcaggaa tcctgaaaca agatagacaa ggaaagtact
      361 gatgattgcg ataaaccagc tttttccagt gaattttcgg atttttgaag ccatattatg
      421 attccattct ttacttaaca atacggggat atccaatgtg atcatataag caatctgttc
      481 accgatactt aaatagttct ttgctgaaaa cccaacgata ataaaacaaa tcgcagacaa
      541 aatacctaaa ataccgttga ctgattttgc cgcattgatg gataaaatac acaatacacc
      601 aagcgtggta ccgataaatg taataacgga cagccaagtg atgggactat tgacaagagt
      661 catgacctgg cagccaagac tgaaaaagaa aagataataa ttttgttgcg gccagccttt
      721 tagctgatga gctaaaaact gaaaatatga cttcatgtat gaggactcct taagacacta
      781 aatgtagtgt gttctgtatt tactaacaca atctattgta tgcttttctt gaaaaaaaac
      841 tagtctttca attataaata atttgtgtta tgataatttt ctgtttgttt tcttgaaaat
      901 ataatttctt actttcattt attatattgg gaacaggaag agtgtcttgg aggacctact
      961 ttctgttttt ttgccttttt tcttataaaa aggaaaaaca gctatttttt tcaatctgac
     1021 acatttacaa tggaactgga gattaatata gatccgagct taaacgaaga ataggagtgc
     1081 tcatatgaac cttacaacga tccatcatat agccatcatt gtttcagatt atcagaaatc
     1141 acgtgatttt tatgttaata aattaggatt tgaagtaatc agagaaaatt accgcaaaga
     1201 acgagatgac tataaattag atctgaaact aggaagtgct gaattggaga tttttggaat
     1261 tgctaatgcg ccacaacgac caaactatcc agaagcttgt ggcttgcgtc atcttgcttt
     1321 caaagtagac gatattgaag aaacaattgc agaattgcag tccttaggga tcgaatcaga
     1381 accgattaga gaagatacct ttacaggaaa aaaaatgacg tttttctttg atccagacgg
     1441 attgccatta gaactgcacg aataaagata acaaaattgt cacttttttt tcgtttcatt
     1501 cgatttttca ttcgttcatt tcttgatatt ctttaaaaaa aagaaacagg agaatgaaga
     1561 atgaaaaaaa tgatgtttgg tgttgctgcc ctcatcgtaa tagggattac cggtatgacg
     1621 ggactgctca atgccgcttt tcacatggat gatattagtg accaggaata tcggatctca
     1681 ttgaaaaaag cagcagcaat cgtaaaaaac gaaacaaaag aacataacat atctgctatt
     1741 cattttgcag ttgaaaaaga tttggagaat cctacgaatg caacatatga ataccagttt
     1801 ttttgtgaag gacaagtcat agcagtcaat ccatccactg ggaaaacaac cgtacgaaat
     1861 gacacggaaa aggccgcaga acatcttttt gatatagaaa aaacggaaga gttcaaacaa
     1921 ccgcaagcag cgatgaagga agcagtcagc tactacggga aaaaagcaaa agtgaaagag
     1981 tggacacttc tagtcaaaga tgaacgtcct tattataaag tccgattaat agatggagaa
     2041 caggaccgtc aggtatggat atctgcatag aagaaggata ttgcttctct tttttacaat
     2101 ataagagtaa gctaagagag taaagatgaa aggaagttga gataacatgg aaaaaatgaa
     2161 atgccctaat tgcggaaaaa aatttgcata cgaagaagtc aataatgtag tcgaacataa
     2221 tgataaagaa atgccagtcg tctgccctta ttgccgaact gaagcggcaa gaatcgttac
     2281 tcatggttat ttcattactg aaaagattga agatttttta aaataacgat tttgcgattt
     2341 ctttttctgc atgttagaat ggagaaaagg agaggaaact atgattcgtt ttgcgagaaa
     2401 agaagatgct ccagaaatcg ccccattgat cttagtgatt ctaaaagata tggaactgcc
     2461 atttttgaag cagacagggg aacaaaagac tttagagatt ttagaagaag caatcactga
     2521 tccaaattat cgttacagtt attcacgagg tatcgtagaa gaaatagacg gtaaagttgc
     2581 aggagttgct tacggttata cagatgaaga agaatcaatc atcgatcagc cactggtacc
     2641 tatcttagag aaatatcagc tagatgcgtc tacacgtttg ttcattgata aagaaacgtt
     2701 tcccaacgaa tggtatttgg actctatcag tgtatcagaa gattttagag gacaaggcat
     2761 cggttcgcga ttattagaag cattacctaa attggcgaag aaagctaatc gctccgtcat
     2821 cggtctaagt gtggatgaaa aaaatccaaa ggctaaaaaa ttatacgaac gccatgactt
     2881 caaagttgtc ggtcagcgga tgatcagtgg tcatctttat gatcatatgc aaaagaatat
     2941 ctaaaaagat gtacctgaaa tgcgtatttc cgcatttcag gtacatcttt ttaacttaag
     3001 ttaagtcggt aagaaacagg cttatcgcga cttttgtctt tacggtagag ccagtaatct
     3061 aaaaccgcag caatacaaat acaaactgga gcatcttcat catttgagac atccaataca
     3121 tagtaatctc ctgaaaaaag tgttgcttta tccattttca tgatttgctg attgaaatga
     3181 tggatttgat attgatgatt atagatatca ccttggactg tccagtgaag tctacgaata
     3241 taataaaagt caccgggcca attgaatatt ttctgcatgg ttcccacctt ttcgaactct
     3301 ttatacagct caaaacgcgt accgaatgtc aggcttatct gcttgatgtg agcaacgaga
     3361 tcgccattca tggcataaag agacaatgca tcgccctttg ttccccagcg accaaccatc
     3421 aaaaacaggg aatgtccttt ttcgtttttg acaattgtcc gagttgcacg tccaagctgc
     3481 ttttcctgaa tgaaaaattc tgacatctat atccctcttt tctagataaa actacaagtc
     3541 ttctgcaatc gcgcgcaata tggcttttcc tacgccagat tcaaggtttg tgtccgttgt
     3601 atatttggca taatctttta cttcgatttc tgcatttccc attgcgaaac tgactcctgc
     3661 tacttgcagc atgctgacat cattgaagtt atcgccaatc gtcatgactt cttctaaaga
     3721 aatcccacgc tcgtgagcga catgggcaac agcaattcct ttttgagcgt ttttatggtt
     3781 cacttcgata ttgttttgac cagaggaagt aacggctaga tcgcctaaaa gagaaatctg
     3841 ctttccaact ggacctaata cagctggccc ttctgtatga aaacaaataa tttttaaaac
     3901 ttccagattc ttttcttcca aaatagcacg aatactgtcc acgtatgtga tgtgcaataa
     3961 ctccagctta gctgaggcca tggcaatcgc cattttatac gtaaggtgag gtaaatgggt
     4021 agccaccatg gaagcaaaac tttcaattcg tttcgcttga ttttcagaat aaagaccatc
     4081 gttggttgct acttcatagt aaataccgtt agcatctaga atatctaaaa cagagattgc
     4141 ttgattattt ggaatcgaca cggtaaacaa tgaatttcct tctttatcaa atacctgtgc
     4201 cccgttcaaa gtgatcatcg cacaatcgat tcctacctca tctaaagctg cgcgagcttc
     4261 ttgatagttt cgaccagtag cgaccatgaa ctcaacaccg gcatcatttg caaaacgtat
     4321 cgcctcggca ttttcaggag aaacactcat atgggcatct aataatgtgc cgtccatatc
     4381 tgatgcaatt aatttgatca taatgattat cgttcctttc tgcttcttgt tcctagttta
     4441 ccatattaat caagtgctga gggtgaagat tgtcccttgt gattgaagaa aatgtgagtt
     4501 attcttttta ttaggaggtg gagtttttga aagaaatcat tcataacagt tggcaagaag
     4561 ttttgtcctc agaattttca aaagattatt atctgcatct aagagaattt ttaaaaaaag
     4621 aatatgcgtc ccaaaagatc catccagata tgtatcatat ctatgaagca ttggaattga
     4681 ctccttatga agaagttaaa gtggtgattt taggacaaga tccttatcat ggagaaaatc
     4741 aagctcatgg cttatctttt tctgtgcaac ctggcgtaaa gatcccgcca tcgttaagaa
     4801 atatctataa agagctttat gatgatcttg gaatcgcacc tgttcaacat ggaaatctag
     4861 tgtcatgggc taaacaggga gtccttttac tcaacaccgt tcttaccgtt cgggaagggc
     4921 aagcgtattc tcatcgcggg aaaggttggg aaagattaac agacacgatc attgaaaaat
     4981 taaatgaacg ggaaaaacca atcgtgttta tcttgtgggg aaaaccggca caagaaaaaa
     5041 tcaaaatgat cgataagagt cgccatatca tcataacatc tccacatccc agtccgttat
     5101 ctgcaagtcg tggctttttt ggttccaaac ccttctcgaa aacgaatgat gcgctccttg
     5161 cgttaggaga agaaccgatt gattggcaac tgcctgaaac agtgtaataa aacagcgctt
     5221 tcctgttata gtgcagttcg ctttgtatgt gaaaaactta atttttccag catttttgac
     5281 tgatacagat aaaataaact gttctttttt tctggaatag tgtatgataa tacatataaa
     5341 aacgtctgga ggtcatcttg tggaattgtt tgatagttta agatttaaaa ttgttcgccg
     5401 tgggatcaaa atcgttttcc cggaagctac tgatgctcgt attttaggcg cagctgcccg
     5461 gttgaaagca gaagaacttg tggaacccat tttagtaggg aacgaagaag aaataagaaa
     5521 tgcagcgcat gccagaggaa tcaaaacatc tagctttacg atcatcagcc ctgataatta
     5581 cgataagtgg gatgaaatgg tggatacttt tgttgaacgt agaaatggaa aggcatccaa
     5641 agaacaagca gaaaagattt taaaagatgt gaattacttt ggtacgatgc ttacgtatat
     5701 ggggattgca gacggaatgg tctctggagc gatccattcg actggcgaca ctgttcgccc
     5761 agcacttcag atcatcaaaa caaagcctgg catcagtcgg acaagcggag cgatgatcat
     5821 ggtacgtgga agagatcaag aaaaatatgt cttttcggat tgtgcgatca acgtgaatcc
     5881 aactgcgcaa gaattggctg aaatcgccgt agattctgca aaaacagcag aattgttcga
     5941 tatcgaacca aaagtagcaa tgatgagctt ctcgacaaaa ggctcagcga aatcgccaga
     6001 agtagacaaa gtagttgaag caactaaaat cgctaaaaaa ctagcgccac aatacatgat
     6061 cgacggcgaa ttgcaatttg atgcttctta tgttccttca gttgcacaac tgaaagcgcc
     6121 agattcagaa gtagctggtc aagcaacagt ctttgtattt ccagaattgc agtcaggaaa
     6181 tatcggctat aaaattgctc agcgtttcgg taactttgaa gcaatcggac caattctaca
     6241 aggcttgaac aaaccggttt ctgatttgtc tcgtggagca aatgaagaag atgtatataa
     6301 attatcgatt atcacagcag cacaaacatt gattgattaa caaatacacg cgaactagcc
     6361 aaaaacagca gctgctgttc ttgactagtt cattttttct ttaaaaaaac ttcattacgc
     6421 gatatactgg caaaaagaaa gaagagcggg agaagatgaa aaatgatcga actatcagga
     6481 ttaacaatga cagaacaatt cgcagaggca attggtcatt ccgcaatgcc aggtgacaat
     6541 ctgatattga ctggtgatct aggtgcgggt aaaacaacgt tgacgaaagg aattgcccaa
     6601 ggattaggga taacgcaaat gatcaaaagt ccgacataca cgattattag agaatatagt
     6661 caaggtcgga ttccgttgta tcatatggat atttatcggg tagcagctag cggagcagat
     6721 ttagggttag atgaatattt tgaaggtgat gggctttccg ttgttgaatg gggaaatcta
     6781 ttagaagaag cactgccgga ggattatttg gaactgattt tagaaaaaag tgatacagat
     6841 ttagaaaagc ggtatgtgaa actaatctca tacggggagc aaagtgaaac cttcaaacag
     6901 cgaattctag aaaaatgggc tgaaaatcat gagtgaagaa gttgggatca tcttgcggga
     6961 agcagtgcca ggagatgcaa aagacattct tttgatgatg ggacaagtta ataaggagac
     7021 agaattttta gttttggatg aagcagaact cctgttacca ccagaaacct tagaagaaga
     7081 gttggattat atatacgaaa gcaataataa tttattgctg cttgcaatct atgaagggac
     7141 gattattgga acagcatctg tcaaggcaga cagtcaattt cgtttgtctc acgtgggaga
     7201 agtcggtatc agtattctgc aggaatattg gggaatggga ctaggcactc ttatgctaga
     7261 agaaatcatc agttgggcga aggaaatggg tgtacttttt cgattggagc ttgacgttca
     7321 agttcgtaat gaaagagccg tacaccttta ccgaaaaatg ggcttccaaa tcgaagcggt
     7381 catgcctaga ggggcaagaa cagatctagg tgaattttta gatgtatata aaatgagtta
     7441 tttgattgaa tagaagaaat aggaaaagag tgagaaactg tcatcctttt gagaaggcag
     7501 aaaaacacac gaagggactg tgacaaaagt cgtgtcgcag tccatttttt ttga
//
LOCUS       DACEMO010000129         7508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEMO010000129 DACEMO010000000
VERSION     DACEMO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.134"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..414
                     /locus_tag="IW728_002044"
     CDS             <1..414
                     /locus_tag="IW728_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1025388.1"
                     /translation="VFEKLEKLSDPVFSKGMMGQGFAIDAKNETIVSPIDGFVSSIFP
                     TKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQKVKAGDILMTINFVVFEKN
                     HKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            450..1322
                     /locus_tag="IW728_002045"
     CDS             450..1322
                     /locus_tag="IW728_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1025389.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            1404..3938
                     /locus_tag="IW728_002046"
     CDS             1404..3938
                     /locus_tag="IW728_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1025390.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            4094..4867
                     /locus_tag="IW728_002047"
     CDS             4094..4867
                     /locus_tag="IW728_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1025391.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            4860..5651
                     /locus_tag="IW728_002048"
     CDS             4860..5651
                     /locus_tag="IW728_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1025392.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            5661..6152
                     /locus_tag="IW728_002049"
     CDS             5661..6152
                     /locus_tag="IW728_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1025393.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            6170..6580
                     /locus_tag="IW728_002050"
     CDS             6170..6580
                     /locus_tag="IW728_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1025394.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            6584..>7508
                     /locus_tag="IW728_002051"
     CDS             6584..>7508
                     /locus_tag="IW728_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HAQ1025395.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIE"
BASE COUNT         2632 a          990 c         1509 g         2377 t
ORIGIN      
        1 gtttttgaaa aactggagaa attaagtgat ccagtctttt caaaaggaat gatgggacaa
       61 ggatttgcaa ttgatgcaaa aaacgaaacg attgtatctc caatcgatgg ttttgtttca
      121 agtatttttc ctacaaaaca tgccttagga ataaaaacaa aatcaggaat tgagatactt
      181 gttcatgtag gaatagatac agttgagcta aatggtgagg gatttgatat taaagttcaa
      241 gaaggacaaa aagttaaggc tggggatatt cttatgacca ttaattttgt agtttttgaa
      301 aagaatcaca agactaaaga tgtcattgtt gtttttccag actaccaaga cgaaattttc
      361 attccgaaac ttgaagaagt aaaacaagga caagaattgt ttgttcttgg ctaatgataa
      421 gtaattaata atagaatcga ggaaaatgta tggtttttgg ggctattgaa gctggtggaa
      481 caaaatttgt ttgtgccatt ggagataaag atttgaacat atttgaaaaa gtcagctttc
      541 ccacgactac acccaaagaa acaatgaatt tagttttaga tttttttaat catcataaag
      601 atgatttaga aggtatcgga gtgggatcgt tcggtcctat tgatattcat tgtgaatcta
      661 aaacgtatgg cttcattaca tcaacgccaa aacttgcttg gcaggatttt gattttattg
      721 gaagtttaaa aaagcaactt aatattccaa ctgcttggac gactgatgta aatgcctctt
      781 gctatggtga atatgtttct ggaaatggca gaggcctttc aagtgttgtt tactataccg
      841 taggaacggg cataggaggt ggcgctatcc aaaacggaaa cttcattgaa ggttttagtc
      901 atccagaaat gggacatatg cttgtaaaac gacacaataa agacaacttt tctgggacat
      961 gtccattcca cggagactgt ttagagggct tagctgctgg tccggcaatc gaaagaagat
     1021 cagggaaaaa gggacaagaa gtgactgaaa atgacgttac ttgggaaata gaagcttatt
     1081 acattgctca gtgtgcatat aatacaacat tgatgttatc acctgatgtc attatatttg
     1141 gtggcggagt aatgaaacaa aaacacttaa aaaagaaagt acaagataag tttattgagt
     1201 tgatcaacgg atatgtcgct attcccgatg ttgacaatta tattatgaca ccaaaattgt
     1261 tagataattc tggtattgta ggttgtttag ctttagcaag agacgttaaa gaaaaatctt
     1321 aatttggaaa aaagatcgtc tcgactattt gtttggttga tgcgatcttt tttttcaatt
     1381 agtaattgaa gggacgccta aaagtgaaaa aaaaaatgaa agatgaagta ttagagtata
     1441 tcaaaaataa tttaaagtat tacgattttt ctgctcaaga tattgcaatg aaattttgta
     1501 ttaaaagaaa tgtcgcttcc cactatttga atcagttatt ttcagatgga aaattactaa
     1561 agaatgattc tgttagacca gtaatgttca agtataatca acaaaagcca aaagactgct
     1621 tttctaagtt tattggagca gacatatctt tgaaatccac tatagataaa tgcaaagcta
     1681 ctgtaatgta tccccctaat ggcctcccat taataataaa aggaaacagt ggcgttggaa
     1741 aaagtttttt agcaagtttg atctatcagt atgctcttga tagaaaagta attcataatg
     1801 acgcaaaatt tgttgttgtg aattgtgcag attatgcaaa taatccagag ctattgtcag
     1861 cagttttatt tggatataaa aaaggagcct ttactggggc tgaaaaagat actgcaggga
     1921 tgctatcttc tgctaatgga ggataccttt ttttagatga ggttcacaat ctaagtgcag
     1981 aaaaccaaga gaaattgttt cttcttatgg attctcaaaa gtatcgtaaa cttggtgaaa
     2041 gtgtgcaatg ggagtatgca aatgttcgcc ttatcttagc cacaacagaa gataaaaata
     2101 cctctttgtt agcaactttt agaagacgca tcccagctga aatcactctg ccagattaca
     2161 ataatagaag tacatctgaa aaaattcaat tattatttga atttcttaaa gatgaagcaa
     2221 ttacaattga ttcaaaaata ttttgttcga ttgaactttt tactgatctt ttatcgaaaa
     2281 catttgaagg aaatgttgga gaattgagga atgaaatcaa gcttctttgt gcagaaggtt
     2341 atttaggaaa taagagaaag tcttcaattt atttagggga caaactagac agcggttttt
     2401 ggatagatcc agaaattagt atagatttaa aaaaaatgtt tcttaaatca ctttctgaaa
     2461 ttgatttgat tgaacttttt caaaatcaca tttcaattga agaaagcatg agtcaattaa
     2521 gagatagaat acttaaagag tgtgcaggtt cttctgagta taattattta gaaagtgacg
     2581 aatttatagc attaagaaac tatgttgtta ataaaatatc accaattatt gattcgacag
     2641 gtctatgttt attagatgaa ttcttggcgg atatcagtct atttataatg tttattgatg
     2701 taattgaaga aacatcaaga aattttttgc tgtctaagtt cagaaaattt cgtatgaaaa
     2761 atgataaaca aaaattattg gcaaatgaga tttggagtag tttagaagtc gaagaaagta
     2821 aacaagaact tttgttgaaa tggatacttt ttcttgtaaa gaagttctat gtgaaaatac
     2881 cagaaaccca ttgtctgata gttatgcacg gtaaaataac agcttctgcg atcgcaaggg
     2941 aaacaaataa gttgctgaat acatatgttt atgaggcttt cgatatgcca attgaaggtg
     3001 agactagtga cttaattaac ctaatcaaca atttttgtaa aagtatagat acgacgaatg
     3061 gactcattct gctagttgat atgggatcat tggaacaaat gtatgaaaaa attgaatcca
     3121 atgtaatagg tgatttggta attttaaaca atgtttctac agctttagcg attgaatgtg
     3181 ggattcaagt ttgccaaaaa aagccaatta gtcattttta ccagatggat tttgattcgt
     3241 ttcaagttaa agttcagtat tataaaggat tatcacaaaa gaaaaatgta atagtatctt
     3301 gtctctctgg tgaggggatt tctgaaaaag ttaaggatat tttaaaacgg tatctcgata
     3361 atcaagtgga gattttgatt tttgatttta acgcattaaa gaaaatagca aaggaaaaag
     3421 atactatgat ttttaagaat acaatttgtg ttctttcaac cactgaattt tacattcaag
     3481 gaatcgattg tttaaattta gaaaatctaa ttaatgggaa tcaaacctta gaaaaattaa
     3541 ataagtatat ggactcggat agctgtgagt tctgcttaaa tgaattagtt aaactcttta
     3601 ctattgaagg cgctagtact aagcttcgct ttttagattc caaaaaagta ttcaatgaga
     3661 tagaaaaagt actatatttg tatgaaaaat attacgatgt caaaatccca agttttctga
     3721 gaattaatct atttctacat ctttctggaa tgattgaacg aattatgatt ggtgatggaa
     3781 taacaaatga acatttgaaa gagggagcgc aactatttga tactttttta aatgtatcaa
     3841 aagatttttt ttcagaaatc aaagatattt actgtattga aatacccaaa ggagagtatg
     3901 agttaatcta tacaattttt gagcagacta ttttctaaat agcatactag cgtctaaaaa
     3961 aatgaaaggg tgtccaaagt atatttagac gctcatttaa ttaatattta ctgtattttc
     4021 ccgctttatt ggataatgtt agttggcaca gtaattgctt atataaaagt ataagaagaa
     4081 aggagagaac aaaatggcgt tttggcaaac atttttagtt gtgttgtatg gatttttcat
     4141 taactgggat aaggaagggc ccaaaatcgg aacctcccaa cctgtcgttg caggtttctt
     4201 gaccggactt attatgggag atattcaaac tggactttat atcggtggga ctttgcaatt
     4261 aatgacacta ggtattacta gttttggtgg tgcatcagta ccggattatc aaacagcagc
     4321 attgatcggt acttatttag caatctcaac taaccaggat gcttctatag gaataacttt
     4381 agcaataccg gtagcaatgt taattgtaca actagatgtt ctaaagtggt caacaaacat
     4441 tttttttcaa caaaaggcag aaaaattcgc acaaaaagaa gattatcaaa aattagagtg
     4501 gatgcaatat ttggcatcaa ttaatacaag ctttgtatct ggaattcctg ttttgttaac
     4561 tgtgatatta ggcccaacgt tagtgggaag tgcaatcaat tatattcctg aatgggttac
     4621 cggtggttta aaggtagcag ggggactatt acctgctgtt gggataggaa tgctacttaa
     4681 atatttacct acaaaagatt actttgccta tttgttagtt ggtttcgttt tggcagttta
     4741 tttccaaata ccaattttag ggatttcgtt acttggcgct gcaatcgcac taatcgtata
     4801 taaaaataga tcaaataata ctcaagtagc accagctgca acaggaggtt ttgacgaaga
     4861 tgaataagaa actgacaaaa atcgacaaac aagtaatttt ccggtggatt ctcaatggtg
     4921 caacttcatt aaattatgaa aaaatggaag ggttgggtta tgcgtattct atgcttcctt
     4981 ttatcaagga cacatataaa gatgatcctg aaggaatgaa acaatccgtt ttagctcacc
     5041 tacaattttt taacactacg ccgtatacag cgccttacat tattggtatg aatgttggaa
     5101 ttgaaaaaga agaaaaaaat aagtctttgg aagcagtaag tgctctgaaa acaggtctaa
     5161 tgggtccttt agcgggaatt ggagatagtg tatttgtagt tattccatgg actatttttg
     5221 gagcaattgc agctaatatg gcattggaag gtagttttgt tggaattatt ttgtggatac
     5281 tggcatcaat tttattaagg attactgctt atcctttata ccttgctggg aaagaatcgg
     5341 gtgcgagcct actaactcaa attgaaacaa aattaaagtc tattactgat gcagtatcaa
     5401 ttttagggtt aatggtggtc ggtgcactca ttgccacagt tgtaaaagtg aatgttggct
     5461 ttgaatttaa acaaggcgac ttaacaatga ccggaaatga aatctttgat caagttatgc
     5521 ctggtttatt acctgcatgt gtagtagcaa ttgtgtatta tttgttagga cgaaaggtta
     5581 aaccagttta tgtaattcta ttaatcttag tagtttctat tgcgttaaga gcgttaaata
     5641 ttttgaagta ggtgttgaat atggatgaaa aaatgaaagg tattatacat gttcgtattg
     5701 atgatcgaat gattcatgga caagtagcaa cacaatggac aaatcaattg agtgctaaca
     5761 gaattatggt tattaataat gaagtagcca atgatgacgt aaaaaaagct gtagttcgcc
     5821 tcgctgcacc acctaatgtt agaacttcaa taattacaag ggaagtagca gttaaaaata
     5881 ttctttctgg taaatatgaa ggacaaaaag ttcttattgt tgcagtttct ccaatcgatg
     5941 ttaaattttt gattgataac ggtttaccaa taacaagtgt aaacgtagga aatttatcaa
     6001 gaaggaatgg aacagaacgc atccgtccga cgataaatat tactgaagaa gagagagagg
     6061 cttttaaaga actaattagt gatggagttg aagtgacagt gatacaaact cctagagatt
     6121 caaaagaatt tttaaaggat tttatttatt aagaacagag gatgagtgga tggagtttat
     6181 aattacatct catggaaata ttgccattgg atttgtagat acattaaaaa tgttttttgg
     6241 cgataattta aattgtcatg tggtaacact tggagatgca ggcattgaag aattccgtga
     6301 aaacattgaa aaaatagttt ctcaagtaaa aaatcaagaa gttatggtat ttgctgactt
     6361 gattggtgga acacctttca acgaatttgc taaaagaagt gaagtatttc aaaatgggtt
     6421 tcaacttctt ggtggcatga atttaggaat tttgattgag gctgtcaatt tgagattaca
     6481 aggaaaaaaa agcgatgata tcatcgaaaa tctaagaaat atgaatacaa ttgcgtgtct
     6541 tgcagatatg caaagacata ctaacgaaga ggatgagtga taaatgcaaa aaatttttta
     6601 caagcctaaa gatttttggt ttggggattg catgcctttt tataaagatg gaacttttta
     6661 tctatttcat caacgagata cgagaaatcc taaaccttta actgatcctt ttgggtggtc
     6721 actagttaca accactgatt ttgtgaaatt tgaagaatac ggggaagtaa ttcatcgtgg
     6781 tggcaacgat gacattgatc aatacattta cgctggaagt attgtccaag gaatcaatga
     6841 gtatttagct ttgtatactg gacataatcg caaagctaag ttggatggaa aaacttcaga
     6901 agtgttaatg attgcaagtt ctaaagattt aattcattgg aaaaaggatg gcgaagcagc
     6961 aaaacttgtt cctcaagaag gatatgataa gaatgattgg cgcgatcctt ttgcaatttt
     7021 tgatcaagat catggaaaat acgttttgat actaggagct agaaaaccag caagtaagaa
     7081 aaagccgacg gggagattgg tctattttga atccgaagat ttaaaagatt ggaatttcaa
     7141 aggagactac tgggctaata atgaatttaa catgattgaa atgcctgata tatttgaaat
     7201 tgatggaacg tggtatttac ttttttcaga gtattgtgaa gataaaaaga ctaaatatcg
     7261 agtcggagca ggattatttt cagattggaa atcaccagta gacgaagctt tcgacggaaa
     7321 agcctattat gcagcaagaa cagtcggaac taatgaaact ggccgttttc taactggctg
     7381 ggtggcaaca aaagaagggg agaacgatca aaataactgg gattggggcg gtgcacttgt
     7441 tccacatcaa attattcaaa agacagacaa aactcttggt gttaaactgc ctgaaaaaat
     7501 aattgaga
//
LOCUS       DACEMO010000130         7443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEMO010000130 DACEMO010000000
VERSION     DACEMO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.135"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(58..1434)
                     /locus_tag="IW728_002052"
     CDS             complement(58..1434)
                     /locus_tag="IW728_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1025396.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1562..2680)
                     /locus_tag="IW728_002053"
     CDS             complement(1562..2680)
                     /locus_tag="IW728_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1025397.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            2822..3256
                     /locus_tag="IW728_002054"
     CDS             2822..3256
                     /locus_tag="IW728_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1025398.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(3334..3867)
                     /locus_tag="IW728_002055"
     CDS             complement(3334..3867)
                     /locus_tag="IW728_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1025399.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(3961..5139)
                     /gene="mutY"
                     /locus_tag="IW728_002056"
     CDS             complement(3961..5139)
                     /gene="mutY"
                     /locus_tag="IW728_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1025400.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(5132..5929)
                     /gene="recX"
                     /locus_tag="IW728_002057"
     CDS             complement(5132..5929)
                     /gene="recX"
                     /locus_tag="IW728_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1025401.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            6036..7418
                     /gene="rlmD"
                     /locus_tag="IW728_002058"
     CDS             6036..7418
                     /gene="rlmD"
                     /locus_tag="IW728_002058"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1025402.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
BASE COUNT         2231 a         1519 c         1302 g         2391 t
ORIGIN      
        1 gaaaagacgt gttgcttgtt tattcaagca acacgtcttt cataaccaag tttccaatta
       61 tcttcttggc tcgtcatctt cgaattcttt tctgaagtat cttcgttctt cttcaggttc
      121 cgcttctgtt tcttccggat catgaaaaat gacacggatt ttaaggattc gagaaccttc
      181 catttcttca gaaaccagcg taatattatc gacatcaaac gataactttt cgccttcatc
      241 cggaatcatg cctaatgctg taatcaaata accagccatc gtatctacat cactcatatg
      301 cagatcactg tcaaaagctt cattgaactc atcaatcagc atacgtcctt ggatgatata
      361 ttcgtgttca tcgatttttt cataaagatt ctcaacttca tctgattcat catcgatttc
      421 tccgacgatt tcttctaaaa ggtcttctag tgttgctaac ccaacgacgc cgccatattc
      481 atctaataaa atagccatct gattctgcgt ctttttcatt tcatataaca gatcatcaat
      541 aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt ttcttgatat ctaaattatc
      601 aaagccgaat ttatgggcag cttttagtaa attctttgta tgaaggattc cgactacttt
      661 atctttgtct tcgttataaa cagggattct tgaatagttt tcggataaaa cttcattcac
      721 attttcttcc acagtgtcgt tgatatcaat cataaatgca tccgttcgtg ggaccatgac
      781 ttctcgggca actttcgtat caagagaaaa aaccccttgg agcatttcta attcttcatt
      841 attcaatacg ccttcgtttt caagcatata acgcatttca tctctggtca tttttgaatc
      901 ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa cttgtggaag cagaaagcag
      961 ccagacgaag ggcttagcta ctacaccaat caagcgtatc agccctgaag tgaactttgc
     1021 gacttcttct gatttattta aagcaatacg tttagggtac aattctccaa aaacaatcga
     1081 tacataggtc aaaatagcca gcacaatgat atttgcaata ttttttgccg ctgtgccgcc
     1141 acctaatacc ggagctaacc gttccgataa agtattagct agtgaagcac cagacaaaat
     1201 gttgaccaaa gtgattccta cttggattgt cgataggaaa ttatttggat tctgcaatat
     1261 ttttaaaagc ttttgagatt tcacatctcc ttcatcagct ttttgctcga ttcgattctt
     1321 attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa gcattgatca atgtcaaaac
     1381 gatcaataat aaaatctgcg ctaacagcga ctgactctca gggtcagcat tcataatcgc
     1441 attctctcct tataaaatta atttttagtt tacgatagaa aaaaacgtgg cactatagcc
     1501 acgttaaaat ggtatcacaa agcaattttc tttgcaataa aaacaagtgt actaaaacta
     1561 cctacgaggt cgtagcaatt ttcttcttgt tttcattttg cttgtcttct ttgacgattt
     1621 tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa aggaacacct aagaagatac
     1681 caagcaatcc ggcaatattt ccagctacta gaagaacgat aatgatcgtt aatggatgga
     1741 ttttcagtga tttcccaatt acattaggat aaatgatatt cccatccaac tgttggacga
     1801 ttagcacaac cacacaacaa agtaatgctt ttaccggatc attgaagact gttactagaa
     1861 atgcaggtgc tagtcctaga taaggtccta aatatggaat taggtttgtc aatcccgcaa
     1921 tgaccccaaa taagaaagca taatccacac caattaccat atatcctaaa aatgtgaagg
     1981 taccaacgaa taaacattcg attgcttgtc cgctaatgta attagccaat gttttattca
     2041 gttgccccaa taactccaca atttggtttc ttcttttttc tgggaaaaac tgttggatgt
     2101 ttggcactag tttttctccg tctttcaaca tatagaacaa gatgaatggt gctgtaatga
     2161 ttactacggt tgccgaagcg atcgttccaa cgatcgaacc taaactattg gatactccac
     2221 taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa ttgctcaaaa tatttattga
     2281 tatctacttc cttgaacaaa ggcaattcgg ctacctcttt taaccagctt tctacctgtt
     2341 tgatgaaatc aggcatatta gatgccaatg aggaaatttg ctgtactaga cttgggatga
     2401 cactaagtaa aatccaaata attgctgcaa ctaaaaggag cagcacaatt gcgacagccc
     2461 atattctttt gatttttgct ttcatcaaca gcttgacaat aggattcaat aaatagtaaa
     2521 gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa aaatgtcccg atcggttcga
     2581 aaataaagtt tatttctgat gaaatcatga tcaatgcagc tatgatcaat aattcaagcg
     2641 accaaaacat tagtttagac tgtctaagtt tttcaaacaa tagatttcct cctttctttc
     2701 ttactagtat atattatatt cgttaaaaat tcttttattc tttttttatt tttgaaaact
     2761 tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag gtggctagac
     2821 catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg ctgtacggat
     2881 caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa tagacaaaga
     2941 tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc aaggaactgt
     3001 acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga ttctttctga
     3061 atacagacac caagggttag gaaaaatatt gattgaagaa gcagaaaact ttgctaagaa
     3121 tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt tttacgaaaa
     3181 actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc atatttatat
     3241 gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc ttttgagaaa
     3301 aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt gcttgtaacg
     3361 gttataccaa atatcaatat agccttccga gaacgggcca tctccattat tgatccagtc
     3421 aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt aattcatcat
     3481 tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt caggaaatac
     3541 cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca aaaaaggtga
     3601 agctagatta caataatagg aaaccccctt ttctctgatc attgctatta cattgaacca
     3661 gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc gtccatcaga
     3721 ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg tttttaatac
     3781 catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt ggatcgtcac
     3841 aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat tttgtgcttt
     3901 gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa tgagctttgc
     3961 ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt tcatttggac
     4021 cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag ttttgttctc
     4081 ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa catgccattt
     4141 cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa caaagagttc
     4201 ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa acagatcgat
     4261 ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg tcacttccat
     4321 cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt caaaataata
     4381 cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg gctttgcttt
     4441 ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac aaaaggcttg
     4501 gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac ctagatccat
     4561 cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca tcgcttcatc
     4621 aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca atctgctgac
     4681 tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga tacttgcaat
     4741 cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag gcgtttgcgg
     4801 catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt tccgggctct
     4861 tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag catttgctag
     4921 ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca ctgtgtctac
     4981 tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac gattatagcg
     5041 ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt gaaattcttt
     5101 tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc ttttccgtaa
     5161 tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc tttactttta
     5221 gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct ccttgtttga
     5281 ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct atcgcttgtt
     5341 ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt ctcaataatt
     5401 ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga tgtgcatttt
     5461 gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca ggtttcaaac
     5521 ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag cgcatattcg
     5581 tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct tttaagcggg
     5641 ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat gttcggattt
     5701 ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa ccgaaatcag
     5761 ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt ccttttaata
     5821 atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca gataaatcta
     5881 cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata aacatcctcc
     5941 tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat tatatgttag
     6001 aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt gtcaaaactg
     6061 gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga atcggttatt
     6121 tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta ctagtgaaaa
     6181 tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa aaaacaagca
     6241 aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt caattacaac
     6301 acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa gcattggaaa
     6361 aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg atggatgagc
     6421 cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag actcaaaaaa
     6481 ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca gactgcttgg
     6541 ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg aataaatttg
     6601 atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc atggttagaa
     6661 tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa aaattcccgc
     6721 aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt tcaatcatgc
     6781 aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc cacttatggg
     6841 gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct cctcgtgcct
     6901 tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc aaagcattag
     6961 atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg atcggcttaa
     7021 gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa gcaatcgctg
     7081 atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa gtcgggacag
     7141 cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt atcatcgtag
     7201 atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa taccctccaa
     7261 aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg gttaatttaa
     7321 gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag actgcacgtt
     7381 gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt gtgaaaaagt
     7441 ctg
//
LOCUS       DACEMO010000131         7439 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEMO010000131 DACEMO010000000
VERSION     DACEMO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.136"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(2..2167)
                     /locus_tag="IW728_002059"
     CDS             complement(2..2167)
                     /locus_tag="IW728_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1025403.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2427..4721
                     /locus_tag="IW728_002060"
     CDS             2427..4721
                     /locus_tag="IW728_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1025404.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            4714..5379
                     /gene="pgmB"
                     /locus_tag="IW728_002061"
     CDS             4714..5379
                     /gene="pgmB"
                     /locus_tag="IW728_002061"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1025405.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            5372..6379
                     /locus_tag="IW728_002062"
     CDS             5372..6379
                     /locus_tag="IW728_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1025406.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            6401..7420
                     /locus_tag="IW728_002063"
     CDS             6401..7420
                     /locus_tag="IW728_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1025407.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         2320 a         1625 c         1400 g         2094 t
ORIGIN      
        1 cttacatttt aatggagccg atcactttac cagctgtcac tgtacctaat tgcttgatca
       61 ctacttcttc tactttatcg tttgtaaata cgatcatgat cgttggatct ttttcttcgt
      121 tttggatcac atcaagatcc atctcagcta atttcgttcc tgttcctact tcttggcctt
      181 ctttgacagc aatctcaaaa gctggctgat tcatttggac tgtatcgatt cccatatgga
      241 tcaaaacttc gataccatct ttcgtttcca gcccaattgc atgtttgcta gggaaaatac
      301 ttgtcacttt tccagcgatc ggcgcaacaa cttctcttga tgttggaata acagcaaagc
      361 cgtcacccat cattttttgt gagaataccg gatcttttac ttcagataaa ggaatcactt
      421 ctccagaagc tgcggaaact agttcttgtt ctatacctaa gtattgctta ctacctgctt
      481 gtccatttcc tgtagaaacc atttctggct ctggaatctc tgctcctgat tgcagcagat
      541 cttcgatatc tgatttcaag acatctgctt ttggtccata aactgcttgg actccattgt
      601 ctttgacgat caatcccatc gcccctgctc gtttccaggc ttcttcagtc cctacttttt
      661 ctcgatcatt tacacttacg cgtaaccgag tcatacaagc atcgacatct ttgatatttt
      721 cttttcctcc cagcaaatga atgatctgga ccacttgctg atctgctgtt cctgttccgc
      781 tgttagatac aacggtttct tcactgtcat tgtcgtaatt tccgttacgt cccggtgtag
      841 caaagttgaa ttttttgatc aagaaatttg ctaagaaata cgtaacaaca ccaaaagcga
      901 tcacacacaa cacgaagttg ataagatctc cacctagacc cgctttgata gctaacggtg
      961 tccgagtcag caattcgata ttcccaaatg aatggacccg caatggcaag atatctgcca
     1021 tcgcaaatgc agccccttgg atcactgaat aaatgacata taatggcaca gcagcaaaca
     1081 taaacatgaa ctccaatggt tctgttacac ctgttagaaa gacagccaac gctgctgaaa
     1141 aatacattga tttatatctt gctttcttat caggatcgac attacgatac atcgcaaaag
     1201 ccattcccat caagatacct gaagaaccaa tcatttgtcc tactttgaaa cgagccggcg
     1261 tccagttatg caacacaaac tcatattgcg acatatcccc tgcaccttta agattcacta
     1321 aatcagtggc ccaagccaac cataacggat cttgcccaaa tacttgtgtc cccgcctgtg
     1381 cgcctgataa aatctcatac gatccgccaa gctgtgtata gttgatagga atcgtcagca
     1441 tatgatgcaa tccaaatggc aatagtaaac gttctaatgt tccaaataaa aacggtgcta
     1501 gaatcggtgc gctttcttgt gattctgcaa tccataaacc aaaattattg attccagcct
     1561 gtacgtttgg ccacaccaca gcaagaatca atgccacgat tgttgaccat aagataacaa
     1621 caaacggaac aaagcgcttt ccattgaaaa atgacaaagc atccggcaat tttctataat
     1681 tatagtactt attataagcc attgccccga caaatccagc gatgatccct acgaatactc
     1741 ccatattcaa agctggtgct tccaatacac ttgtaaaaaa tcctttaacc atgatcttcg
     1801 taccgaacag agtgtgggta aatgcatctt cattcgcaag catgtcactt gtgacaccaa
     1861 agatagcccc agtgatccga ttgattaaaa taaatgagat cccagctgca aatgctccgc
     1921 ctgctctttc ttttgcccaa cttcctccta ttgctaaagc aaataaaata tgaagattac
     1981 caataattgc ccaaccaata ttttcaatta ctccgcccgt tgtgacaagc ggcgtccaat
     2041 caggattgat caagggaatt gatttcccta tactgatcat caacccagct gccggcataa
     2101 ctgctaccac aaccattaaa gcctttccaa atttttgcca aaactcaaaa ctaaatatct
     2161 ttttcattcc gtttagctcc tttacgcaat cggtttcgtt aaacgtatta tagcgtgcgt
     2221 tttctttttt gtaaatgctt tattttttat ttttctgtct ataccattct ataaaaccta
     2281 taatacgtgt gttcccttct ataattttct cctaaaaatt tattttttta agcttttttc
     2341 tatgaaatat tttcttttgt ctattgactt gtgcaatcgg ttgcgtttat tatgaacgta
     2401 taaaacagct cattaggagg agaaaaatga aacagatcaa acgactattt gctattgatc
     2461 catggaaaat cagcacgaca caattagata aaaaaaatct tcgactgcaa gaatctttaa
     2521 caagtattgg aaacggttat atggggatgc gcgggaactt tgaagaaact tactcagggg
     2581 accaccatca aggtacttat ttggcgggtg tctggtatcc cgataagaca cgtgtcggct
     2641 ggtggaaaaa tggttatcca gaatactttg gaaaagtgat caacgccttg aactttatcg
     2701 cgatagatat ttcgatcaac gatcagctga tcgacttggc ttctttggaa ccagaagact
     2761 tttactggga attagatatg aaaaatggtg tattatctcg tacattcaca atcactactt
     2821 ctactaataa agtacgcatt tccttcgaac gttttctgag cattgtcaaa aaagaatcag
     2881 cttatatcta tttaactata gaaatgcttg aaggtagcgg aacagtcaaa ctcgtatcaa
     2941 aattggatgg aaatgttcaa aacgaagaca gcaactatga tgaacatttt tggaatgaga
     3001 ttgatcgtgg aactatagaa gaaatcggct atttgacgac taaaactatt ccaaacaact
     3061 ttgggattga acaatttact gttactgcag caatgcgcca tttcgttaat ggaaaatcag
     3121 tcatccctga ctatcacgaa gatactttag cattacatgg ttgcttgtct gcagaattat
     3181 ctcaaggtga gacatggtca gtggaaaaag aaattatcgt tcttactagt agagatattc
     3241 cagaagcgca gcaagcagaa aaagctgtct cagaattaac gaaccagaca aacaattata
     3301 tccaagcaaa agaagagcaa acagcggctt gggcagaacg atggaagctt gccgatgtgg
     3361 tcatcgaagg agacgaagaa gcacaacaag gtattcgatt caatttgttc caactattct
     3421 ctacttacta cggcgaagat gaacgattga atatcggccc caaaggattc acaggagaaa
     3481 aatacggcgg agccacttac tgggatacag aagcgtatgc tgtccctctt tatcttgctt
     3541 tagccaaacc tgaagtaact aagaatctgc tgaaatatcg gcataatcaa ctgccgcaag
     3601 caatccacaa cgctcagcaa caaggattaa aaggcgcgct ttatcctatg gtcaccttca
     3661 ctggtgtcga atgccataac gagtgggaaa tcacattcga ggagattcac cgaaatggcg
     3721 cgattgccta tgcaatctac aattatacga attacaccgg cgatacggaa tatttgaaaa
     3781 atgaaggact ggaagtttta gcagaaatcg cacgcttctg ggcagaccgt gtccattttt
     3841 caaaacgagc aaataaatac atgatccacg gtgtgaccgg cccaaacgaa tatgagaaca
     3901 atatcaataa caattggtat acaaatacga tcgcagcttg ggtcttgcgt tacacgcgag
     3961 aaaattattt aacttatcaa aaagaagctg ctatcactat cagcgaagaa gaactagcca
     4021 aatggaaaga tatcgcagag aacatgtact tcccagttga cgaagaatta ggaatctttg
     4081 tccagcatga cacattctta gataaagatc tcatgcctgt atccgatatt cccacaagcg
     4141 atcttcctct gaatcagcat tggtcttggg acaaaatcct tagatcttgc ttcatcaagc
     4201 aggcagatgt actacagggg atttatttct tcaatgatac ttttacaaaa gaagaaaagc
     4261 aaaaaaactt tgatttctat gaaccaatga ctgtccatga gtcttccctt tcgccaagta
     4321 tccattcgat tttagcagca gaattaggaa tggaaagcaa agctgtagaa atgtaccagc
     4381 gaacagctcg cttggatttg gataattaca ataacgacac agaagacggc ttgcacatca
     4441 cttccatgac tggtagctgg ttgtcgattg tccaaggatt tgctcaaatg aaaactgccc
     4501 atgatcagtt gagattcgca ccatttctgc caaaaacttg gaaagcttat tctttccata
     4561 tcaattatcg cggacgttta ttgttcatct cagtcacaga tgaacaagtt actttcgagt
     4621 tattagaagg ttctcctcta gaaatttacg tctatgaaca gtcttattct ttagcggatc
     4681 gtctagaaat aacgctaaga aaggaggaag caaatgttta aaggggtatt gtttgattta
     4741 gacggtgtca ttaccgatac tgctgaattc cattatcgtg cttggcgaaa gcttggtcag
     4801 gaaatcggca ttacgatcga ccacacattc aacgaacagc taaaaggcgt cagccgagaa
     4861 gactcactct ctttgctttt agcttatggc ggaaaagaac actctttttc aaaggaagaa
     4921 tttgcagaat tagcgaaaag aaaaaacgat tattatttag agatgatcca gacgatcgaa
     4981 ccaaaagatg tcttccccgg ggtcgtgcct ttactggatt ctttaaaaga agaaaaaatc
     5041 aagattgctc ttgcttctgc aagtaaaaac gggccattcc tacttgaaaa aatgggtctt
     5101 acaccttact ttgatgctat tgccgacccg gcagaagcaa caaacggaaa acctgcgcca
     5161 gatatcttcc tccttgcagc aaaagctgtc ggactaaaag cagaagaatg tatcgggatc
     5221 gaagacgcgc aagccggtat ccaagccatc ctctcaagtg gcgctcaacc aattggcgtg
     5281 ggacgtgctg aagaccttgg agaggagatc ccgcttgtcc ctgatacgac ttttcttacg
     5341 atcgattact tgaaagaaaa gtggcatgac catggataat ccagagatct ccgtcgtacc
     5401 agttccagag actgaaataa agaaaatcac attgaagaac gactacttag aagtcgttct
     5461 tctcaattac ggtgcccgcc tgcaccaaat cttcgcacca gacaaagaag gaaaatcaga
     5521 aaatgtttta ttgtcttacg atcgtttcga agatgttcta acagacaaaa gcttttttgg
     5581 agcaactgtc ggacctgtag ccggaaggat ccgggatgct tcttggggtg agcatagatt
     5641 ggagaaaaac tgcggatcac accatatcca cggtggcacg aaaggctggt ctttccaatt
     5701 ttgggaagta gaagttttca aaactccatc atcaataggt gtggtctttt acttaaaaga
     5761 tgaattttct acctatccag gaccaatcac tgctacgatc acttaccgtt taacaaaaaa
     5821 cgaattagag atgacaacct ctgctagtag ccttgttgaa accatctgca atccgaccaa
     5881 ccatgcctac ttcaaccttt caggaaatgg aacaagagac atctatgaac atcagttaac
     5941 ggtcttttta gatggtgtgc ttgaattaga tcaagaaaaa ctgcctactg gaaattggac
     6001 aaaaaaagaa aaactgccaa tcgactttag aaaatcccca acactgcaag agatccttgc
     6061 atgttatcca gatggactag atgatgtatt tcttttgcat catcctaggc tttccaaaac
     6121 ttccttacaa ttattcgaga agcattctgg tcgccaaatg actatagcaa cttctaataa
     6181 aagtatggta ttattttcta cgactggatt cgaagctgat ttctctgtca atggaaaaca
     6241 gatgcattca aactacggat tagcaatcga accgcaagaa atccctgata tcgttcattt
     6301 ccccaaattt ggatcgatca acctccatcc tggtcaagaa cggatcagtc agacgattta
     6361 tcggttctct gcacaataac aagagaaagg tgtttttatc atggctatta ccgtaaaaga
     6421 tgtggcgaaa aaagccggtg ttgccacctc gaccgtctct cgtgtcatca acgatcatcc
     6481 tagtatttcc gagacaacaa agaagaaagt ccgaaaaatt atggatgaac taggttatgt
     6541 tcctaacatg actgcccgca atctaggaaa acgcatttcc agtgccatcg gcgtaatctt
     6601 accccctctt gattcaaaag agcggatcgg taacccattt tatcttgaga tcatggaagc
     6661 aatcaacgaa gaagcgcgga attatgatat gactacagca atcgctactg ctaaaaattt
     6721 tgaattgctg ttagagaacg tccagcgtat gcatttacaa aaacaagtag atggttttat
     6781 tctagtgtat tcagatagtc aagatactgt cattgattat ttataccaaa accacatccc
     6841 tttcactttg atcggacagc cttatcatca tgagaacgaa atcgtttatg tagataatga
     6901 taaccagctt ctaggtaaac aagcaacaga atttttgatc cagaatggac ataagaatat
     6961 cctgtttgtg acaaacacga ctcatgaaag tttgtatttc gaacgatatt tcggttatca
     7021 aaaagcaatg atgatggctg gtcttcctgc gttatccagt gtagtactag agcatccgga
     7081 ggattatctg tcctttgaga aaacgttgaa agaaaacgaa gccacagcat tggtggtcat
     7141 tgacgatatc ttcgcccttc gcacgatgca gttggcgcaa atgtatggct atgtcattcc
     7201 agagacactc tccgtcatta gcttcaacaa ctcgatcttc tcaacattaa cacaccctta
     7261 ccttacaagt atcgatatcg atatctccga attaggacgt gtcgctatgc aaaagctgcg
     7321 cgaattgatc catgaagaat tgagcagtgg cgtacgtttg gtcatccctc atcgcttgat
     7381 caaaagagag acagtcgttc ctttagaaaa cccatactaa atagctgact cactaaata
//
LOCUS       DACEMO010000132         7406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEMO010000132 DACEMO010000000
VERSION     DACEMO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.137"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..771
                     /locus_tag="IW728_002064"
     CDS             <1..771
                     /locus_tag="IW728_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate permease"
                     /protein_id="HAQ1025408.1"
                     /translation="PLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGMLKKIG
                     EKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEHLLKS
                     LLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGVAKTD
                     WLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGGTGAL
                     AILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            797..1810
                     /gene="citC"
                     /locus_tag="IW728_002065"
     CDS             797..1810
                     /gene="citC"
                     /locus_tag="IW728_002065"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ1025409.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            1814..2107
                     /gene="citD"
                     /locus_tag="IW728_002066"
     CDS             1814..2107
                     /gene="citD"
                     /locus_tag="IW728_002066"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ1025410.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            2112..3017
                     /gene="citE"
                     /locus_tag="IW728_002067"
     CDS             2112..3017
                     /gene="citE"
                     /locus_tag="IW728_002067"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ1025411.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            3010..4569
                     /gene="citF"
                     /locus_tag="IW728_002068"
     CDS             3010..4569
                     /gene="citF"
                     /locus_tag="IW728_002068"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ1025412.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            4566..5123
                     /gene="citX"
                     /locus_tag="IW728_002069"
     CDS             4566..5123
                     /gene="citX"
                     /locus_tag="IW728_002069"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ1025413.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            5116..5979
                     /gene="citG"
                     /locus_tag="IW728_002070"
     CDS             5116..5979
                     /gene="citG"
                     /locus_tag="IW728_002070"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ1025414.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            6213..6622
                     /gene="tnpA"
                     /locus_tag="IW728_002071"
                     /pseudo
     CDS             6213..6622
                     /gene="tnpA"
                     /locus_tag="IW728_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(7259..>7406)
                     /locus_tag="IW728_002072"
     CDS             complement(7259..>7406)
                     /locus_tag="IW728_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1025415.1"
                     /translation="LNQEVRRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYI
                     KFAK"
BASE COUNT         2604 a         1171 c         1492 g         2139 t
ORIGIN      
        1 ccattatcga ttggttatgc agcaatatta tctggtatgg gacaaaatga tatctttggg
       61 aagattctac cgtgtgtgat gttgggaagt ttagtcgcag ttctactgtc tggtatgctc
      121 aaaaaaattg gagagaaata cccacaatta aatggtcagg gtaatttgat cgatggggat
      181 gatgaacatt tagagaactt gaagggttca gctaaaccag tcaatattga aaaaacattg
      241 ttagcaggcg tggtagcaat aacgatttat tttgttgctg tctatgttga gcacttatta
      301 aaatcactcc ttggcttttc tattccttca cctgtgatct tattagtctc tcttatgggg
      361 gctaaaatgc ttggttttat ccctaaaaat atcgaagaag gtggaaattc gttgtatgga
      421 ttcactgttc gtgggatcac acctccctta ctttttggcg taggggttgc aaaaacagat
      481 tggttagagc tgatcgaagt atttaaaaat cctgcaacga tagtaacaat cattgtgacg
      541 gtagtagcta ttgtcgctac agcttttatc gccgcaaaat ttttaaaaat gtacccagtt
      601 gatactgcca tcgcagtttc atgttgtagc ggacaaggcg gtacaggtgc tttagctatc
      661 ttagctgcgg gtgatcgcat ggaattaatg ccatttgcac aagttgctgt tcgactaggg
      721 ggtgctatga ccgtaacgtt cgctatcttt ttaatgggtc tgctatcata agaacataaa
      781 gaaaggacgt gaccatgtgt ttgatcaaac agatatccaa cgattatttt taaaaaataa
      841 ctatatcaaa gaccaatggg aaaatctttt gcttgattca ggaatacaaa agaagcaaat
      901 cgaagatttt caacatttag atcaaacatt gggtatatac cataaagaaa agttagtagg
      961 aacagtatct tatcaaaaca acgtgattaa atatatcgcc gtgtctgaaa aatataaaga
     1021 tggtggacat acatttaatg tactagtctc tgcaataatt caggcaatga gtcaacaaga
     1081 gatttttcat tatattgttt tcacgaaacc gcgctatcaa aaaagtttta actttttagg
     1141 ttttgaaaca atcgtatcta ccccaaaaat agctgtatta gaaatgggag atagttctat
     1201 aaaacagtac ctagagagga ttcctaaagt gaatggtgaa aaaaaagcag caattgtcat
     1261 gaatgcgaac ccatttacag aagggcatta ttatttagta aaacaagctg ccaataacaa
     1321 cgattgggta tatgtatttg tactcgaaaa agaacaaaat cttttttcga cgaaggaccg
     1381 actcaaatta gttcaacaag gattacaaaa actgaaaaat gtcattgttc tatcaggtgg
     1441 cgagtatatt attagccctg caacgtttcc cacctatttt ttacgagaag atgatgaaat
     1501 agcaaaggag caaatggtcg tagatgctac tttattcaaa gaaagaattg ccacttattt
     1561 agatatctct gctagatatg tgggagaaga accactttcc ccaatgactc gttcatataa
     1621 cgacatactg gaaacgattt taccccctga aataaaagtt catgtccttg caaggaagaa
     1681 aacggaacaa caccaagtta tttctgcttc tcaagttcgt aaagcgtatc ttgctgggca
     1741 attggaaaaa ataaaatata tggttccaga aacaacctat caatacttaa aaaataaaag
     1801 ggagcgttaa gtaatggaaa taaataaatc agcgattgct ggaacattag agtcaagtga
     1861 tgtccaaatc atgatttcac cgaatacaga aggaatagaa atcgatttag acagtcaagt
     1921 gattgagcaa tttggaaaac aaatcaaaaa agttatctat gacacactag cagtttatca
     1981 gattaaaaac gtaaaactca aggtagtaga taaaggggca ttagattgcg ttattgttgc
     2041 acgattaaaa acagcgatcc atcgttcctt aggtatccaa caccaagaaa caccgtggga
     2101 ggtttaatta gatgatgaaa gaacgtttga gaagaacaat gatgtttgtt ccaggaaata
     2161 atccagcgat gatcaaagat gcaggtattt atggtgctga ctcgattatg ttcgacctgg
     2221 aagatgcagt gtctatggca gaaaaagaca gcgcaagaat gcttgtggct gaggcattga
     2281 aaacagtgga ttatggtgac acagaattaa ttgttcgaat aaatgacctg cactcagaat
     2341 ttggacgtgc agacattttt gcaatggtta aagccggcgc gcatgtatta cgtttaccta
     2401 aaacagaaac ctctgaagat ataacagaag tagaaaaagt gattatagaa gcagaaaact
     2461 atcataatgt tccgataggg cgaacattga tgatggcagc cattgaaggt gcttctggtg
     2521 tattgaacgc acctgcgatc gccaaagcat ctgatcgatt gattggaatc gccattgggg
     2581 cagaagacta tgtgacaaat atgaaaacca aacgatatcc agatggaaac gaattgtttt
     2641 ttgctcgcag tatgatcgtt catgctgcac gtgcagcagg catttccgca tttgacactg
     2701 tttactctga tgtggatgat atggaaggat ttttaaacga agtacgttta atccatcaat
     2761 taggttttga tggaaaatct tgtatcaatc cacggcaaat tccagtagtc aatactgttt
     2821 atcagccgtc aatcaaagaa atcaaacgat cactagacat catctcggca atcgaagaag
     2881 caaaagaaaa aggttctggc gtaatttctt taaatggaaa aatgatcgat aaaccaattg
     2941 tagaacgtgc acaacggaca ttgatgttag caaaagcatc acgattgatt gatgaggagg
     3001 gaaatatcca tgcataataa acaaagacgt agcattccat caagtgtttt aacaaagtat
     3061 aaaaaagaat tccaaagtgt ttcattatcg ccacaaacga ttcaacgttg tgcaccaaaa
     3121 gtaacgactg taactaatca gacaaataaa ttgatgcact cgatcaaaga agctatagaa
     3181 aaatcgggct taaaagatgg aatgacgatt tcatttcatc atcatttgag agaaggtgat
     3241 tttgttttta accaagtgat ggccgtgatc cgtgaaatgg gcatcaaaga attgacctta
     3301 gcaccaagtt ctttaacgaa cactatgaat gagatggtta ttgaatgcat caaggacggt
     3361 acagtaactc atatcacaag cagtgggatg cgcggcagct taggtgaatt tatttctcat
     3421 ggaggaatgg acaatcctgt gattttacgt tctcatggta atcgtccacg tgcaattgaa
     3481 agtggtgaat tgaaaatcga tgttgcattt ctaggagtac caacatctga tgattatgga
     3541 aatgctaatg cgactgttgg aaaatccgtt tttggttcat tagggtatgc aaaagtagat
     3601 gcacaatatg cagataaagt cattcttcta actgatcatt tagttcctta ccctaatacg
     3661 cccattagta ttccgcaaac acaagtcgac tatgtcgtgg aagtagaagc aattggtgat
     3721 ccgaataaaa tcggtgctgg tgctacacgg tttactaaaa acccaaaaga attaaaaatt
     3781 gctgaaatgg tcaaagatgt cattgttcac tcaccttatt tcaaagatgg cttttctttt
     3841 caaacaggaa cgggtggtgc agctttagca gtaacgcgtt acttagctag tgaaatggaa
     3901 gaaaaacaga tcaaagcttc ttttgcatta ggtggcatca ctaaaccaac ggttgattta
     3961 ttggaaaaag gtcttgttga aaaagtgtta gatgtccaag actttgacaa aggagcagct
     4021 gaaggaatgg cgcggcttga gaaccaacag gagatcgatg cttcctttta tgctgatcca
     4081 agtaataaag gagcggttgt taaccaatta gatgtcgcta ttttatctgc attagaaatt
     4141 gacacaaact tcaatgtaaa tgttatgact ggttcagatg gtgtgctaag aggtgcgatt
     4201 ggtggacatc aagatgcagc aacggctaaa cttactatca tctcagcgcc gttagtacgt
     4261 ggccgaatac ctacagtagt aaatgatgtt actacattga tcacaccagg gaaaagtatc
     4321 gatgtattag tcacagaagt aggggttgct atcaatcccc aacgtaaaga tctaatagct
     4381 atttttgaaa gaattccaca aattcctgtt tttacaatag aagaattaca gcaaaaagca
     4441 gaaaaaatag taggcatccc ggaacctttg caattcacag atcggatagt tgcctatgta
     4501 gaataccgag atggttcaat tttagatgtc gttcgtcagg tgaaagaatt tgaagaagag
     4561 agatcatgat gtttgatact ggagaaattc aagaaatcga ggatattttg atccataaag
     4621 aacaacgtgc taacttgcaa agcaacctgc tttccaagta ttcaaatagt tcattgatgg
     4681 tctttacatt gaatattcct gggccgatca aaaataatcc aatgattgag caggtatttc
     4741 gtcagggaca gcagtcaatt cagcaaatgt ttttaaaaaa aaaggtggaa atgacgtaca
     4801 caaaagaaat caatttgcca agtggattgg actggtacgt aatagtaaca gaagcacctt
     4861 tgaatctcaa aaaatggttg gtcacgttag aagagcaaga cgcattaggt agactttttg
     4921 atttagatgt cttgttcaaa aaaggagagc agataacaat tgtcagtcgc caagctctta
     4981 atcatgggga acgtccttgt tttatttgtg ggaaatcagc aaaggtatgt gcacgtaatc
     5041 gtacacatac tgtgaaagaa atgcaaaaga aaatcgaaca aatagtagcc gattccttaa
     5101 aaggagcatt gaaacatgca tgataatatt caaaaaatag cacaagaagc acaattagcg
     5161 atgctttatg aagttacatg tatcaataaa ccaggattgg ttgatccagt agaccccggg
     5221 tctcatcaag atatggacat ttttactttt ctacaaagta gtgtagtttt gactccctat
     5281 tttgaagaat ttatccaaac aggattggac cgacaacatc aaccaattga agaaaccttt
     5341 atggcgattc gacagattgg tttagaggct gaaactgcga tgttctcagt gacaaatggt
     5401 ataaatacac ataaaggtgc tatcttttca ttggggattt ttcttgctat ttgtggccgg
     5461 ttaactattt ggaaagttcc ttgtaaaaaa agtttctttc aaaaagaaat tcaaaaaatg
     5521 acgaaaaatc tacttaacga ttttgggaag attgatcaaa caaaaccaaa agcatggact
     5581 tggggagaat atctatttgt taaccatgga ttgacaggga tacgagggga agccaaaaga
     5641 ggttatcctt gtgtttttga ccgaggattg ccttattatc aagcatatca agggtcgcaa
     5701 caagataaga tgatagacac tttacttttt ctatcaatgg aagtagaaga taccaatttg
     5761 atcaaacgaa gtaataatag acatgttttt gaagagtatc aattacttat tcgtccttat
     5821 tttgaattag ggggtgtaaa aaccatacaa ggtaaaaaat atttggatta cctaaatcag
     5881 caatttaaaa agaaaaattg gagtattggt ggaagtgcag acttattgat ccttacaatc
     5941 tttttagata aaatcatgga caaaggatgg ttatgttgat actataatac attattttgt
     6001 attagtaagc taataactaa aaaatggttc aatacaaata ttttataaaa aatggtagtg
     6061 cctatccttt cagattgtag acaaagaaaa atttctttgt ctacaatttt tttgttaatg
     6121 acttcagtca gtttcgtgcg caaagcgtgc gaaacaagaa aaccatgcgt ttctacacgt
     6181 ggaagaacta gcatatcgaa aggagttttt tcatgtcaaa tgacgataaa agtttagccc
     6241 atactcgttg gaattttaag tatcatattg tatttacacc aaaatatcga cgaaaagtaa
     6301 tctataggca attacgaaaa gatattggaa aaatacttag gaagttgtgc aaaataaaaa
     6361 acgtggaaat tatagaagca catgcaacgc cagatcatat tcacatgctt gttcaagtac
     6421 catcaaaaat aagtatatcc agttttatag ttatctgaaa gatcgtagtg ctgtgctaat
     6481 ccacgagcag cacgtaaatc acaaatatag atacggaaat aaatcatttt ggtcggagga
     6541 atattatgtg agtacagtcg aattgaatca aaagacaatt gaaaaataca tccgtgaaca
     6601 agaagctgat gatcgcattt gagatagtat aagtaaaaga gagtagatgt tgcaataaca
     6661 aaagggtaaa aatggactac ctccagtaaa ataccgaaaa atagtttaac ttttggagat
     6721 aagtccattt aagggtctat agaatgacaa tttttttagg ggattttact attattactt
     6781 caagacccgc ttggattcgc aaagtgagtg aactcttaaa actacctgat agtaggttgt
     6841 tttaagagtt atctgaaagt aaaaattgca tttatatttt tgataaatac gtaaatttat
     6901 atatacgtat ttatcaaagt tttaggccaa aggaatattg tgtaaataca ctaggattga
     6961 atgaatagac cagaagaaat tgcgtccaag aacaaaagaa gcatgatagt gctctatata
     7021 aattgggcat tcaagaatct gaaatttaaa ggtagaattt tagttagact tttagatttt
     7081 aaacacaaaa ataaccatac aagcaacaag aagcaaagtg ctatattata aattgtccgt
     7141 ccgtttactt acataagatc attggggcta tttttaaaag agaataatag ataataaatg
     7201 aaaaaaaagt caagtccaca atcctgtgta aaatactata caatgctctc actatttttt
     7261 acttggcaaa tttaatatat tttcttgatg aagaaagcca ctcatcgtgt tgatcaataa
     7321 ggactgctcc aattaaacga ttggctgaaa cgcagtttgg aaagatacga ataatttttt
     7381 ctcttctgcg cacttcttga ttcagt
//
LOCUS       DACEMO010000133         7406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEMO010000133 DACEMO010000000
VERSION     DACEMO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.138"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(71..796)
                     /locus_tag="IW728_002073"
     CDS             complement(71..796)
                     /locus_tag="IW728_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025416.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(950..1276)
                     /locus_tag="IW728_002074"
     CDS             complement(950..1276)
                     /locus_tag="IW728_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025417.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1313..1678)
                     /locus_tag="IW728_002075"
     CDS             complement(1313..1678)
                     /locus_tag="IW728_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1025418.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1715..1930)
                     /locus_tag="IW728_002076"
     CDS             complement(1715..1930)
                     /locus_tag="IW728_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025419.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2059..2394
                     /locus_tag="IW728_002077"
     CDS             2059..2394
                     /locus_tag="IW728_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025420.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2470..4467)
                     /locus_tag="IW728_002078"
     CDS             complement(2470..4467)
                     /locus_tag="IW728_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025421.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4565..4840)
                     /locus_tag="IW728_002079"
     CDS             complement(4565..4840)
                     /locus_tag="IW728_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1025422.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5072..5806
                     /locus_tag="IW728_002080"
     CDS             5072..5806
                     /locus_tag="IW728_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025423.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5845..6987
                     /locus_tag="IW728_002081"
     CDS             5845..6987
                     /locus_tag="IW728_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1025424.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2486 a         1352 c         1072 g         2496 t
ORIGIN      
        1 ttggttcctc tcaaaacacg gtcatataac aacggggtag ggtaaataaa tgaaaaagat
       61 ggaagaatca ttattcaata acttttttaa atacttcgtt gtaatcagtt acctgtccgt
      121 tcttaaatac cacagaataa tcttttttcc cattttcaag taaccgaagt ggaatatctt
      181 taccaacttt ctctgtcact gcattactga tacctgttag attagctaat aaatctgata
      241 aagattgagc atctgtagaa atctctgtat cagtatttat cgtgattcct ttatcttcat
      301 ttacaagttt ccaagtaggt ggtatagctg tgtatgtttc tacaaatgaa ttaagttttt
      361 cattaagtga cttctctttt atatttccat ctaaaaactc tgtatatatg gcttgagcta
      421 tactaaaaca aagctggcct tgttctaata attttacatc attagttagt aaagctgtgt
      481 tcacgttatc aatcgcttca agaagagtat ctttggtgtc cctaacagct ttaggtgtgc
      541 cttctggaat ttccctactt gatacctcgt ccattttatc ttccataaac cccttaaaat
      601 catatctttc tcccaaatcc atagaagttt ttgagaaagc tttcatttct gagtctattt
      661 cttcaataat atttaacatg tctgatacat attgtttagg ctgattcgtt tttaatctat
      721 atgtggtaaa tatctcaatt cctccaatta tcaataaaat tgccaaacaa atccataata
      781 attttttctt tttcattttt cctcctatac actgctgtta taacagtgac taatgtaaag
      841 attttcccag caatcatcag actattattt acagggaatc aaaaaaactc cttagtaagc
      901 ttacaccttt aggagttcct gtttgaacgg tatgcataat acatttaaat caatttaaca
      961 ttaacacctc agtatatggt atctctacat atacacggtc tttttttgga tagtactcga
     1021 catttatatt gatagcactt ccattgtcta acgtagcttt agcccggtac gtgttatcac
     1081 cattatcatg agtgatcgtc acatcatcac atgtccgatt taagttatac tgttcttgta
     1141 aaatctgagt taccaatggt acagatgact tctctagtcc agatgaaccc gtcattcttg
     1201 aaaaaattat acctataata gccaatacaa cgatacctat aatgacaaac ttcttattca
     1261 tatttacttt attcaacttt ttatctccta tctccaaaat gattttctct catcagtaac
     1321 tcattgacat ccccaatagt acaccaaaga tggctccagc tacccctgct atcatcaaaa
     1381 tcgtacccat ttgttcatct ttctctcgat ataagtaacc cataataaca gcaacagcac
     1441 caaataccac aggaataaat gctaatgata ctaccataga aatccaacca ataatcaaat
     1501 acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta attgtctgct
     1561 cagtactttt ctttactgtg tctgttccgg ttgtctcatc tgtttcttgt aatgctccgc
     1621 aaaactggca atacttactt tcatttgtta tttctttacc acattttcta caatacatgt
     1681 cttctcctcc taaaatcatt caattgctta ttagctaact gtttacttta ttaaatacat
     1741 cagaaaacag ctcatttatt ggtacactta atatctcaga aagcattttc atttgatagg
     1801 gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta acattcattt
     1861 ttttggctaa ctcagtttgg gaaagtcctt tttcttttct gtatcggctg agattactta
     1921 cttttttcat gattctcccc tcctgtttta ttatatacaa caatatgatg tatttcaact
     1981 cataaaatat ataaatactt caaatgtcat tacaaataca acaacttgtt gtactctact
     2041 gagaaggggg gtgtattaat gaaacagaag caatatgcaa aggtcattag agaaaagcga
     2101 aaagcactcg ggtggacaca aaaagaatta gctaaaaaaa tcttctctac acaacaggca
     2161 gtagcaagat gggaaaattc tgtaacagaa cctaacctcg atagcttaac agctctttct
     2221 agagccctag gaacccctgt aagccatttt ctagacaatg ttgacataga ttatgaggaa
     2281 gagtttttgg ctctctatcg ctctcttagt actgaagatg caattcgaac aattgactat
     2341 atgaagctat taaaaaggca agaaaatgaa cgcaatcagc tattgaaaga ttgaaaaata
     2401 aataacccac cgaactttaa cggtgggtgt attgagatga aataagatta agagctatgg
     2461 ataacctgtc tagtttaata taattgggtc tcttctattc cttactctcc gtccatcatc
     2521 tcttgttaca cttgtaaatt gataaatagg aactaattca actcctgata atatcaattc
     2581 ttttattact tcatctatcc ctgccccttt cgctagatat actaaaaaac tactgcttgc
     2641 tttcacattg tatagtttct taatatcctt agaagctcgt tcaagagtga atccttgttt
     2701 aacaaagtag cctctgtttt ctattaaagc atttttcacg ctgttatccc tgacatttcg
     2761 taggcgttca caaacagtgt taataagttc tttttctgtc attagatatg tcccttgcca
     2821 cgataagccc tgtaaaactt catgtgcttc atctaaaatc aattgttttt ccttgctaag
     2881 ttctgatcgg gaattttgag gaaatactct tttagcctct ttttcgttaa aacatatctt
     2941 aacccattca taagaaaaat attgatatgt aaaatgaaat ttttccagaa gtggaaggat
     3001 atcttcattc aatttttgaa tcacagaagt tgcattaaaa acgtctaatc gatagacatt
     3061 gcttctctta tgtttaaagt ttcctaacgc tttctcttcc tttaacctat ttaggtattc
     3121 gagcatcttc aaaggaacat cttcatcatc taattttttt atcaatccta taaaagtcat
     3181 aagtgatagt aacttattac atttatctat gtcataagtt ttaccatagt aagaactcat
     3241 ttttttagct aattgttccc ccgacaaaat acaagtcaca ctttccttac tatcatcaaa
     3301 aaaattaaca tcagaaagca agacatcata gacacgtcgc atttcctcta gataattcct
     3361 tattgtcctg aaaagtttgt ctttcattga ttcaagagtg tcaaaaagta cttttgattc
     3421 ctctttcaat tttcgaaaat gtgtaggata caagtctagg ttctctaata gccaattaaa
     3481 tgtttctacc cgactgtctt tctttaaaat tagcatcaca actccaataa aattttcact
     3541 gatatcacaa ttactggaat gacaataaaa aatggaatgc ccagttttac tggtaaaaat
     3601 actagcagag ggattatgat cgttgtgaaa cagacatctt aagtttttac ttgacttaag
     3661 taatttattc atatcaatgg ttaacaatct ttcataaatc tcattaaaag acatgtcgga
     3721 ggaatcaaac aggcagtctt taaaccactg tttcatttct tctttgtcgt cattttttat
     3781 caacttaaca aaatcggttt cattgactag ttcatactct ctccctgaat cactttgaca
     3841 atttttagaa catgtcatgg taaaatcaat cggaagctca gtaatatcta acatgttgtc
     3901 ttcattaatt attatcactg catctgactt atatcctcca aaaaacattc tattcgaagc
     3961 atttacattg tcatcacacc cggggatgtt ctttttcaag tattcgtaaa cagcaaccat
     4021 tttaccatag tcagtaattg cttcttttaa tagaaagaca atcttgtatt tttctagatt
     4081 ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa ttatgaacta aattgataga
     4141 taaaaccttt tctatgctta agtatctact atcatcttta ctcaaatcta ctaccttccc
     4201 cttctcatct ttaacagtgt tatcgacatc cagaataatt actctggttt ccacaaaact
     4261 ctccttgtca ttgcctttat tctttgtctt gcttggaata aatgattgtg atgaatttag
     4321 tatctctaca aactcatctc ttgtaacttc tctatagtta tctaaacacc tatctcttac
     4381 atcagttttt tctttttttg taactggctt ctctaataat ttttctttat ctacataaat
     4441 tggaatatta atcattgatg tttccatttg tgtttcctcc ttataaaatt ctgttaaaga
     4501 ctaaaacata cagataataa aaacaaactc tttattacta tatgttttag tcttatttgt
     4561 ttactcaacc tttataccgt tccatgaaac tatcaatatc agatttttta aatctaatga
     4621 ccgtatcaat ttttataaca ggtaacccgt attgagaaac atattttcta atcgttccta
     4681 ctgatacacc tagatattca cttgcctgct gatagtccat atactctaga gtgactccag
     4741 aaataatgtc ttctagcata ggctttagca ctgtaattat ctctttcact tgttgttcaa
     4801 cttgtataga aacaatgcta tcaatgattt gtagagacat atttccccct cctttcgtga
     4861 ttgcttgaac aaattttcta agaattatct tcgtattttt tgctcaaaat aaaagtattt
     4921 tgttattaac cgattaatca aacaaaaaat gattaccagt tcatcgacat acacataata
     4981 cctcgataaa aaaggggtgt caatagtttt gtaaaattta tttacaacac cccttttttg
     5041 aggtatacta attttaggag gtgtggtcaa tatgataaaa cgtccaccaa taaattattt
     5101 agaacgaaaa aagatactcg gaacaaaaat aaaagctata agaaagagta aaaaattaac
     5161 acaaccagca tttggtctta tgattaacaa cggtcaatta attgacaaaa agactatcta
     5221 cgaatgggag aagggaacct atttaccaat accagagcga ttatcccgaa ttgctgattt
     5281 gggaaatatg agtatagagg agttagtttg tggcaatgta gaagaatata ttttgggaat
     5341 tattctttat agagatagca ttgtactaga tggtataaca tttccagata aaaacctgtt
     5401 ccagcatctt cgtcaacagt tcccaccagt acactctaat ttagatacat ggctggatag
     5461 atattccaaa ttagaaccag agatgcaaga atttattgct aacaagactt gtaataaagt
     5521 taaaaatgaa aaaattagtc ttttcaatat cttaaaaata gaagaattat tcatcaatgc
     5581 tattgtagaa gaatttgaca ataacattct tttcctcact agcagtatag aagaactact
     5641 agaaagaatg gtagatgaat ggctacctat tcaattgaaa gacatgtctt atcccgaaga
     5701 agctgtgaga gaaattactg ataatataaa caagctagaa caaacgattt catcaattgg
     5761 gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc atctagtggt aagtactcct
     5821 tgtaaactaa gaggagatga tttcatggca aaaatatcaa agtacaaaaa gaaaaacggt
     5881 caagtggcgt ggatgtataa aggacacatt gccaccgatc cacgaactgg tgaaaaaata
     5941 aatactacga gaagaggttt tagtagcaaa caagaagctc aaaaagatta tgacgagtac
     6001 agacatcaaa tattatacgg tgttaaaaag aaagcacctg atatgacatt tgaagacctg
     6061 tataatgaat ggataactca tcagcgtacc agtgttaaag cttcaacaat cgcaatctca
     6121 gtaagatacg caaataatca aatacttcca gcatttggta agcttaaact atcgaatatc
     6181 tctgtcccat attgtcaaaa agtggtagat gaatggcaca gtaaatatga aagttacgat
     6241 tatatgagaa aacagactgc acaaatttta cgctacgggg ttgcaatgca atatattgac
     6301 aataatccta tggaaaaaac acttcttcca cgaaaaaaag agtatgagaa aaatcgtaaa
     6361 ttttactcaa aagaggagct taataattta ctggatgctt ttaaagattt tggtaacatg
     6421 aaacaatacg ccttcttcag attattggca tacacaggta tgagaaagag tgaagtactc
     6481 gctttacagt ggaaagacat agacacgttt aataaagaac tacatgtcaa taaaactctt
     6541 gctgtagatg aatttggtaa agttataatt caatctccga aaacaagagc ttctagacga
     6601 gttatctctt tagatactga gactctttct atcctcaaca actggaaatt acagcaaaaa
     6661 gaagagtatt taaaacttgg atacaatact agctctaaag agcaacatgt ttttacaaca
     6721 gttaaaaaca ctctatacat tccaaacact gttaatgatt ggttaagata tattttgaaa
     6781 aaatataact tgcctcgtat tacacctcat gggtttagac atacacatgc tagtttatta
     6841 ttagaagcag gagaatctgt caaggtagtt caacaacgat tagggcatga aaatagtaaa
     6901 gtgacattag acatctatgc acatattaca aataatgcac ctaagaaaac aggtcaagac
     6961 tttgctgata tgatggctca tcagtaaaag agctggtcaa aatctggtca tttctagtat
     7021 acaggcaaaa caaaaaagct agaaccctta tatatcaagg gttctagctc ctataacagg
     7081 tttacccaac actgccttcc aatgactctt ttgggaatca gaggaaagaa tcaggcttta
     7141 tagagatatt tcatctaaca aattacttta gactgacagt atatatcagc aggtactgtc
     7201 atcgggaacg tagccaatat cgtatccaaa acagcaggta gagaagctat ttgaggattc
     7261 aagttattct atttcaacca tgaagttaag ttcattaaaa ataaagatag ttggtatttc
     7321 aataattttt gtatagagtt aatcacttta ttgatgctaa ttcatcttga ttggtattat
     7381 aatcgtattc aagttgcgtg gcgtgc
//
LOCUS       DACEMO010000134         7365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEMO010000134 DACEMO010000000
VERSION     DACEMO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.139"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..698
                     /locus_tag="IW728_002082"
     CDS             <1..698
                     /locus_tag="IW728_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1025425.1"
                     /translation="KNTILYNVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLP
                     YFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIG
                     YNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRA
                     DFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITN
                     TIARRFDSDSALF"
     gene            710..1630
                     /locus_tag="IW728_002083"
     CDS             710..1630
                     /locus_tag="IW728_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1025426.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1671..3128
                     /locus_tag="IW728_002084"
     CDS             1671..3128
                     /locus_tag="IW728_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1025427.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3473..4087
                     /locus_tag="IW728_002085"
     CDS             3473..4087
                     /locus_tag="IW728_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1025428.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4080..5804
                     /locus_tag="IW728_002086"
     CDS             4080..5804
                     /locus_tag="IW728_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1025429.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5801..7252
                     /locus_tag="IW728_002087"
     CDS             5801..7252
                     /locus_tag="IW728_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1025430.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2517 a         1224 c         1468 g         2156 t
ORIGIN      
        1 ctaaaaatac aattttatat aatgtagcat ttatcctttt gaatctgctg atctcagtcg
       61 tatttgcaat cattatgagc gagttgagaa ataaacgact cgttaaagtt tatcaaacga
      121 tgtccttact accttatttt ctatcttggg tcattatcag ttactttgtt tacgcttttt
      181 taagtcctga caaaggaata ttcaatcagt ggatcactgg acatggagga gaagctgtca
      241 attggtataa tgaaccgaaa tattggccgt ttatcttggt gttcattggt acatggaaag
      301 ggattggtta taacagcatt atttactttg catctgttat gggaatcgat ccgacttatt
      361 atgaagctgc tatggttgat ggagctagca aatggcagca gatcaaacat gtgacgatac
      421 cacaacttat tccactaatg acgattttaa cgattttagc cgtaggaaac attttccgag
      481 ctgattttgg cctgttttat aacattccta ggaattccgg tgcattatat gatgtgactc
      541 aagttttgga tacgtatatc tacaatggcc tgacatccac aggagatttt ggtatgacgg
      601 ctgctgcagg actttaccaa tcagttgtcg gctttgtctt gttgatgatc acaaatacga
      661 tcgcccgacg ttttgatagc gattctgcat tattctaagg aggaaaaaaa tgaaaaaaaa
      721 gaaagtggag agagtccaag tccgctcttt caataagacg gctaattttt tctctaatat
      781 aatgattgca ctctttgcta tttcctgtat tttgccattt atatttgtta tcgccatctc
      841 ttttaccgat gagtccatat tgtcgactca cgggtacagt ttttggccag aaaagttcag
      901 tatatttggt tatacgtact tgttcgacca gatgcaggat aaaattttcc aagcgctttt
      961 cgtcactgta cttgttactg ttttaggtac actgatcaat agcacggcta cttctttata
     1021 tgcttatgcg atttcacggt caaactttcc attccgtcgt ttttttaccg ttttttgttt
     1081 gattacgatg ttgttctcac cagggatggt ggccaattat cttgttatga caaatcttct
     1141 tcagttaaaa gatacgattt gggcattgat cttgccaatg gctgtcagtc ccttcaatat
     1201 tattgtgatg cgcacattct ttaaacgttc ggtctctgat tcgattattg aatcggcacg
     1261 gatcgatgga gctagcgaac tgcgaatatt tgttcagatc gttttaccgt tagctgttcc
     1321 aggtattgcg acaatcagtt tatttgccgc attgggttac tggaatgact ggttcaatgc
     1381 tttactatac atccaagatg ataagctagt tccactacag tatcttttga tgaaaatcca
     1441 aagtaatatt caatatctga cacaaaattc aggagcagga agccaattga caggcggatt
     1501 agcctcagtt ccaggagaat ctgccagaat ggcgatcgta gtcatctcga cgttgcctat
     1561 tgcgatcagc tatccatttt tccagaagca ttttgtcaaa ggtctaacaa tcggcggagt
     1621 aaaagaataa agaaacaaaa atataaatca taaaattagg aggaagaaaa atgaagttat
     1681 ggaagaaact agcctttaca ggtgtttcag cgttgttgtt gggaacatta gcagcatgcg
     1741 gaggttctgg ttctaaagac caggcagaag ctagcaattc ggacactttg cagatgtatc
     1801 aaatcggaga taaaccagat aatttcgatc aattgatgga agtagcaaac aaacgaatcg
     1861 aaaaagagat cggtgtaaaa gtcaatatca attacattgg ctggggcgat tacgaaaaga
     1921 aaatgaacgt catcatttct tccggggaaa attatgatat cgcctttgcg aacaactatg
     1981 tttccaatgc ccaaaaagga gcctttgctg atttgactga attagcgcct aagtacgctg
     2041 aaaaagctta taatgattta gatgaagcat atattaaagg aaatctggtc aatggaaaat
     2101 tatatgcttt cccagtcaat ggaaatgtct ttgctcaaca agtacttact tttaacaaag
     2161 ctttactaga taaatacgat ctttcaattg atggaatcca atcttacgct gatgcagaaa
     2221 aagtcttgaa ggaattccat gaaaaagaac caaacacggc agcttttgcc attggacaag
     2281 gcttcaaagt ccaagggaac tttgattatc ctttagggaa tacgttacca tttgctattg
     2341 atctggatgg agatgattcg aaaatcatca accaatacga taatgaaacg tatatcggac
     2401 tgttacgtac aatgcatagc tggtacaaac aaggactcat tccaagtgac gctgcaacga
     2461 gcaataagga ttatccacta gaaggaaata cttggctaat gcgtggagaa acacaaggac
     2521 cgtatgatta tggcgataca attttgacaa atgcagcaaa acaagaactt gtctcaaaag
     2581 caattactgt tccactaaaa tctacagctc aagcacaaat ggcaaatttt gtcgtatcaa
     2641 atacttcaaa aaataaagaa aaatcagtag aacttctagg gctgcttaac agtgatccag
     2701 aattattgaa tgggcttgtt tgggggatcg agggagaagc atgggaaaaa gtcgatggtt
     2761 cagatgataa aatcaaattg ctagatggtt atcagccaaa tacacatatg tctgcttgga
     2821 atacaggaaa caacaagatt ctatatacac aagagtctat tacggatgac atgattgcca
     2881 aacgtgatca atcgattgct gatgcagaaa cttcaccgat tttaggattc agcttcaaca
     2941 ctgacagtgt caaaacagaa ttaagtaata tttctaatgt catgaatcaa tatttagatg
     3001 gattgaatac agggactgtt gatccagatg aaacattacc gaaattgaaa gacgctttgg
     3061 acaaggctgg ctatgataag gtattgaaag aaatgcaaaa acaatatgat gagttccgac
     3121 aagaataata atgaataaag acaaaggggt tgtgacaaaa gacgtgtcac aacctcttgt
     3181 tccgaataaa cggtgttcaa aagccagttt ctcggtatta agctaaatat atgtaaaaat
     3241 gtggaaagcc atattcacaa aatttttctt actactcgaa actaagcggc tatttcacaa
     3301 actcttggta aacggtgttc aatcagctaa ccctcggtat tcgctcacaa aacgacaaaa
     3361 tatgaggaac tatttttccg tttgtttctc taataatcag gtcaaggtgc tgattgccca
     3421 accttttttt gtataatgag aaagtataaa cttaatgttt ggaggagaag atatgttagg
     3481 gaaggttttg gaagagttat ttatccggat ctgggtcatt ataaaactaa ccttgtattt
     3541 ctggatatat acatttgccg gcgggatcat ctttggatta ggtgcggcat ggaaaacagt
     3601 caatgaacta ttttatttgt acggattcga gtataaagaa atcacaataa aaagaggctg
     3661 gaatatatat aaaagaaatt ttttaagagg caatctgcta tttagtttat ttttatctgg
     3721 tactgcttta ttgagttaca atttatattt atctgttcaa atcaaagggc tcattttttt
     3781 agctattgat tttattttac tttttgcctt gctctgccta tttgctactt atcaatacag
     3841 tatgatttta gatagtgaat atacgatctc tgttcctaat ctgctaaagc taagttttat
     3901 ttcggttttt tccagctttt cgtcttttct aaaaataatt attggtagcg gtataatttt
     3961 aggcgtgact tggcaattca aaggattgat cctgtttggt gtcattggtc ttttgacggt
     4021 ttggaatggt acgatgacaa cacattggcg tgaagaatta gataaacagt tggaaagtta
     4081 tgaataagtt tcggattcta ttttcaaaag gaaccttact aaatcgtctg ttgcggaaat
     4141 acgcggttat catgatcggt gtaacgatga ctgctaccgt tatttttagt atccatactt
     4201 gggaacaaaa tcaaaaacag gcagaaaaca tgacaagtga tgccgttcaa agcacaagca
     4261 gaatgctgaa tgataaaaca accttgagta ggatcatcaa aaaccagctt gtagggaatt
     4321 ccgaaaagat cgaaaatgtg acgacctact taacaaaacc catagatcag tatctgatgt
     4381 atgtatacga acagcaaaac agtaccgatg aattagtttc tttcccaaat caaatcaaag
     4441 atctctacgc aaattatgaa gaactaagtg cgatatatat cgtgttgaat caactaccgg
     4501 agtattatga atcttctaga gaaaacaaag gtggggaaat aaaaaagggg acaccaaaat
     4561 tactagacgc cttttatatc aagttgccta tcacacaagg aggagctgag tcaggaagta
     4621 tttttattgg ttttcaaaag aaagaactag atatgatttt agaaaatcta acgtcattca
     4681 atggactctc tttatacatg atttctggaa cgacaaatcg actatacaca ttccatgata
     4741 ataaaatagc taaagaaacg tttgaaagac aagaaatgat catcacagag agtctgaaga
     4801 aaaattcaca attgccgatt gacgatctgg aaaaaaacaa ttttgttcaa caccaagaac
     4861 tgtctgatga ttatcgtata ttagctgtac tagataaaaa aagtgtaaaa atcgaaacgg
     4921 gaaaaaatct tgccccgctg atcattggta tcattatttt agacaatata ctgcttatct
     4981 ttctttataa aacattcaaa cgatattcac agcaagtatc tatcgtcatg caggccatgg
     5041 atcaaacagc agcagggaat ttagagacgc ggatcgacat aacaaacacg gaatatgaac
     5101 tgaaggaact ttcaatcggt atcaacgaaa tgttggatag catcaatcag tttgtagaag
     5161 acatctacaa attagaaatc aaacagcaag acgcacatat gcgggcgctt caagcacaga
     5221 tcaatccaca ctttttatat aatacattag aatatatccg aatgtatgca atcagcgaag
     5281 gaagtgaaga attagcagat gttgtgtatg cgttttccgc tttgcttcga aacaatacga
     5341 atcaagagaa aacgatcact ttaaaagaag aactggactt ttgcgaaaaa tatgtctatt
     5401 tgtatcaaat gcgttatcct aatcgagtag cctatcattt tatgatcgat cctgatttgg
     5461 aaaagatcga agtacctaaa tttgtgatcc agccattagt tgaaaattat tttaaacacg
     5521 gtattgattt tactcgattc gataatgcgc taagtgtcaa agtcttgcag gaaggcaaac
     5581 gggtaaggat catcatcaaa gataatggaa aagggatgac tgaaaaacgg ctaaaacaga
     5641 ttgaagaaaa actgtctcat cctaaagtgg aacttcacgg ttctatcggt ctgcaaaacg
     5701 tgaatgaacg tttacgtgcc agcttcggca gcagttatta tatgtcgctt gagaataatg
     5761 agacaggtgg actgaccgtt tctattacat tcaaggaggg atgagcgtgt atcaagtgtt
     5821 attggtagat gatgaataca tgattttgaa tgggttaaaa aagataattg attggcaaag
     5881 tttaggcttt caaatagtag caacagcaga gaatgccaaa gaaggactag ctgtacttga
     5941 acagcgacag attgatttag tagtaacgga tgtgacaatg ccagaaataa atggactgga
     6001 atttattgaa gcggctcaaa aagaacgtca taattttgaa tttatgattt tatcaggata
     6061 ccaagaattc gattatttga aaggaggtat gcaattaggc gccgtgaatt atttgatgaa
     6121 acccgtgaat aagttcgaat tggttgaaag cctcaagaaa atcaaaacac ggttagatca
     6181 gcaaaatgaa caaaaaaatc aacaagaaat ctatcaagaa ctgttattca gtcaatggtt
     6241 gaatgaagaa ttggatgaaa taagtgaaga agaaataata ggacaaataa gtgagaagca
     6301 gcataggata gtattgattc aactggcaag aactcatgat actcttatca atagatggtt
     6361 gcaagaacgg caacaaatct tttactatca acgaaattac ggagaccgtg tattgatcac
     6421 gttgttgttg gaggcagatg aagttgatgg cttctgttgt tttgttcaag aaaactttcc
     6481 tgatcaggaa tggctgatca gtatcgggga agagacagat tctttggaca aaatccctga
     6541 aacattccaa caagcgaaag acaatctaca gttgcatcaa ttttatggag ataaagacga
     6601 gcatgttttt tacgcagtac aagcagaaac aaaagatcaa accatcgatt tttcaagttt
     6661 cagacgcgtg ctgcaaaata aacaattgga agtcgctcaa aaaatgatca ctgatttttt
     6721 tgaacagttt cagttagcag ccatgatgcc ggaagatata cgatattcca gttttttact
     6781 gtttatggag attcaacggg aattgattga tctggaagat gaggaatatt tacaaggaat
     6841 cgaaaaaatc catcaggcaa aaacggtgca agaacttcag cagctacttc tgtcattcat
     6901 caaaaagcat cagagacaga aaaagtacag tgacaatgtg gaaaaagtaa tcgtaatcct
     6961 gcaccagcat tatcaagaac cacttacact caaagaagtg tcagaaaacc tgcatttaaa
     7021 tgtcatgtat ctaggacagc tgttcaaaaa agaaacgaaa aaaagttttt cagcttattt
     7081 gaaccattta cgtatggaaa aagcgaaaca actgcttttg cacagtaatc aaaacatcaa
     7141 tgagatagca agtgagattg gctacaacaa caccacgtat ttctctaaat tattcaagaa
     7201 aatcgtcggc agatccccta aagagtaccg agaaaacagt ggagaatcat aaaagaaaaa
     7261 gccaatgtaa tgaaataact tataaaaaat tgtctagaaa tctaattttg ggctgttctt
     7321 attacttgag gcaaggcagc tttttcatga cctctcgata aacgg
//
LOCUS       DACEMO010000135         7331 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEMO010000135 DACEMO010000000
VERSION     DACEMO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.140"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            290..919
                     /locus_tag="IW728_002088"
     CDS             290..919
                     /locus_tag="IW728_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025431.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(1043..2152)
                     /gene="ugpC"
                     /locus_tag="IW728_002089"
     CDS             complement(1043..2152)
                     /gene="ugpC"
                     /locus_tag="IW728_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1025432.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            2361..2783
                     /gene="mgsA"
                     /locus_tag="IW728_002090"
     CDS             2361..2783
                     /gene="mgsA"
                     /locus_tag="IW728_002090"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1025433.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            3106..3642
                     /locus_tag="IW728_002091"
     CDS             3106..3642
                     /locus_tag="IW728_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1025434.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(3682..5445)
                     /locus_tag="IW728_002092"
     CDS             complement(3682..5445)
                     /locus_tag="IW728_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025435.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(5448..7163)
                     /locus_tag="IW728_002093"
     CDS             complement(5448..7163)
                     /locus_tag="IW728_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025436.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         2205 a         1399 c         1440 g         2287 t
ORIGIN      
        1 taactctaag tcacaaggaa caatttcaaa aaacagcttc tcatgttttt gagttagttg
       61 gtcttattac ttgatgcaag cttttgacca ctattatgcc gaacaaggag atgtgacaca
      121 aattttgtca catctcctat ttttgtttct tgatcaaaaa attcttatac ttgatactca
      181 cactcatttt gtatggattt agaaaagaat atgtgtcttg acagttatta gtaagtgagt
      241 tatactaagt cgacatttat gtgtcaagac atattaggag agagaaaaaa tgagaaaaaa
      301 ttcagttcga aaactaacga tttctgcttt attgattggg atgggggtca ttatcccaat
      361 ggtcatgcct aaaatcatga ttccaccagc ttcctttact ttggcgtctc atgtgccatt
      421 atttattgcg atgtttttta caccaggagt tgcagtggct gttgcgctag gtacgacatt
      481 tggtttcttt ttaacaacgc cggttatcat cgcattgcga gcattgtcac atcttgtgtt
      541 tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt atcgttttaa aaaatggaca
      601 gtttactttt gctaattggc gtttccaagg gttcaatttt gtgatcggtg tgattcattc
      661 tttagcagaa atgctagtag tcagcatctt ttactttatt ggtaatatgc ctgaaacata
      721 ttattctcaa ggatattttt atacagtatt catcttgatg gggattggtg ggcttatcca
      781 cagtttagtt gattataata ttgcttattt tatagcagga acactcagca aatattttga
      841 tatccccgta tttacggcag caaaaaaaga aaatcggaaa gagtcgattc aagggtacgc
      901 gaagaaaacg acatattaga catgatctct caaaaaaaaa actctcatct agtaatggat
      961 aataaatcct ctaagatgag agttttttgt cctaaaaaga gctgcaccaa catcttctgg
     1021 cacagctcta acagggggca atttatggaa gtttgatgac ttgttctgtt tctttatcaa
     1081 agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt ttatggaaat
     1141 ctcttgcatc tacttttgag atgaactcag tagaaccagt ttttgtgtaa agcatcgttt
     1201 cagctccaag taattcggaa acgacaactt ctgatttcac tacggcttca ggtgaagctt
     1261 caagggctac ttgttcactg tggatgtctt ctggacgaat accgaagata acttcttttc
     1321 cttcataacc tttttcaact aatactttgt tttttccttc aggaatacga agcttaagcc
     1381 catgtccatc tgtgataaca ccatttttca atgtcacatt aaagaagttc atggcaggag
     1441 atccaataaa gccggcaacg aacatattga cgggtgtatc ataaacttct ttcggtgtac
     1501 cgatttgttg gataaatccg tctttcatga taacgattcg gtcagccata gtcattgctt
     1561 ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag acgctggtga agtttggcaa
     1621 tctcagcacg catggctaca cgtaatttag catctaaatt tgataatggc tcatccatta
     1681 agaagacttt tgcatcccga acgattgccc gtcccaatgc cacacgttgg cgttgtccac
     1741 cagataaagc agctggtttt cgtttcaagt attctgtcag acctaagatc tctgctgcat
     1801 tgtccacacg ttttttgatg tctgctttat catatttacg taatttcaag ccaaaagcca
     1861 tattatcaaa aacagtcata tgaggataca atgcgtagtt ttggaaaacc atggcaatat
     1921 cacggtcttt aggtgccacg tcattcatga cttttccgcc gatatttagt tctccttcag
     1981 agatatcttc cagtcccgcg atcattcgca aagtagttga ctttccacaa ccggaaggac
     2041 caacaaaaac gataaattca cggtctttga ttgatagatt aaaatcagtt acagaataat
     2101 tttctgcatt gtcatatttt ttatatacgt ttttcagagc catttctacc ataatacgtt
     2161 ccacactttc ttttttagtt ttattaacta tttaaagtat aaatgaaagc gttaactaac
     2221 ttcaatgtca tgttgaacaa gaattttttc ttttttttgt caatgtgcta gttttaagct
     2281 ttaataaata tccataagaa ataaaaactg atacactgga gtaaataagt atgttaacaa
     2341 tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga aaaaaaccta
     2401 tgatggtgaa attagcaaca gcgtataaaa caatattgga aaaacatgaa ctttttgcta
     2461 ccgggacgac tgggcagagg atcacggaag caaccggctt gccgatccat cggtttaaat
     2521 ccggaccgtt aggcggggat cagcaaattg gagccatgat ttcggaggat cagttagatt
     2581 tggtgatttt tcttcgagac ccgctggctg cacagcccca tgaaccagat gtaacagcac
     2641 ttatccgttt atctgatgtc tatgagattc cgttagctac gaatatcgga acagctgagg
     2701 tgctactaag aggactggaa gccggcttcc tagattttcg aagaatcgtc catgaaatag
     2761 acaagcatcc actttcattt tgatggataa gcgtatggaa aaataacgtt taatgttcgc
     2821 ctatatgtag ctataaaagc taaacaaact gataatatgc gatataatac gaacgtattg
     2881 gataatataa acaataatat tagtttttcg tatttgcagt aaatgtgaaa atatgctata
     2941 gtttctgtgg gaaaagatcc caaaaataat acagagtaga aaataaagcg ttaagtgcta
     3001 agaggatggg atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt attttcgcat
     3061 tcgctgcatg atacgatgta gcagctctta aaggagatgc taaaaatgaa gaaacaccgg
     3121 ttagacgcac gtatgattgc tattatgggc ttattgattg cattgatggt cactttgtcc
     3181 cgactggtag caatcgagac cccatttatt aaaatcagtg tcacatttat tccacaagtg
     3241 atcatgggca ttctttttgg tcctttttgg tcagggatag gcgcagtttt agctgattta
     3301 gtaggaatgg ccctcttctc gaaatctgca ttctttattg gatttacatt gaatgcattt
     3361 attgaaggag caatctacgg attctttttc tatcgaaaag agatcacttg gaaaaatgcc
     3421 attctggcta cgcttagtgt cacgctgatc atcaacttat ttttgacacc attatggctt
     3481 gcgttgatgt accatgtgcc attgttcagc tgggtcgttt gggcgccccg cctgttaaaa
     3541 acagtaatat ggttaccgat tcaatcaatt gccatttatt acgtaggacg tagtattcct
     3601 tacaaaaaaa ttttaagaag tttagcgatc cacgctaaat aaaaaacaag cagtcttccc
     3661 atcccagaag actgcttgtt tttattgtgc tgtgaattgt gcttgataga gccgtcggta
     3721 ataaccatct ttttgaagca gctcatcatg ggtgcccatt tcgacaatag ctccctgatc
     3781 catcactaat atctggtccg catttttgat tgtggacagg cgatgggcaa tcacaaaact
     3841 ggtttttcct tccatcattg ctaagaaggc ttgttggatt tttttctctg tcaatgtatc
     3901 gactgagctg gtagcttcat ctaagatcaa cataggggga ttactgatca tcgttcgagc
     3961 aatcgtcaac agttggcgtt gtccatctga tatctttaac ccttgaccgc caatcttagt
     4021 atctaatttt tccggcaaac gttctacaaa ttcatacata taagaggctt ttagggcatc
     4081 attgatttct gcgtcacttg cttccggatt cccaaaacga agattttctc gcaatgtact
     4141 gtcaaatagc caagtatctt gtaacaccat tccaaaactt ttacgtaatg tatcacgttg
     4201 gatagagcgg atgtcatggt catcgactgt aattgttcct tttgttgtgt cataaaaacg
     4261 catcagcaaa ttaacaagtg tggattttcc ggcccctgtt tttcctacta tagcaatggt
     4321 ttgacctgga taagcagtaa aattgaaatc ttcgatcaaa cgctgcttgg ggtcataaga
     4381 aaaagaaaca tgatcaaaag agacttttcc tttgatattt ttgggttcca aggcataggc
     4441 atctggcaaa tcaggctttt cttctggctg atcgatcaaa tcaaatgctc gttctagacc
     4501 agaaaaagcg gtttgcagtt gtgtcgtgat tccagaaagt tcgataaatg gtttagaaaa
     4561 ctgacttgag tagatcgtaa agctggaaat cacaccaaca gtgattgacg aaccactctt
     4621 caatgccaat aggccgccaa ctaaaccaat cgataaataa gcaaggtgat ctacaaaacg
     4681 agaagacggg tttgtcaagg aagaagaaaa ttgtgcccgt tgtccttttt catatagatc
     4741 agcatttagc agttcaaatt gctgttgact agcttcttct tgctgaaaag ctttcacgat
     4801 tttctggtta ccgattcgtt cggtcacaaa gcccgagata tccccaataa tcctttgctg
     4861 tctatcgaaa tttgtttgtg aagcacgagc aacgatccaa ctcacaagga aaataattgg
     4921 tgtagaacaa agaacgacca atgtcagaag tggacttaga cgcatcataa aaataaacga
     4981 aagaatgatg attgccactc cagaaaataa ttgattgaag gccgcagaaa ttgcaatgga
     5041 aatattgtcc atatcatttg taaatcgact gacgatattt ccgtgggagt tttgatcata
     5101 ataacgaagt ggcaatcgat tcagatgagc aaatgcatct tttcttaatt ttgcaacgga
     5161 taagtaagct aaccgattgc ttaacacttg gatgagccat tgactgacta cgttgattaa
     5221 aacgatacca ccgaatagaa taagtaaatg gaacagtcga ttgaagcgga cctgtccttc
     5281 tccaatcatt tgatcgatac tttgaccaat ttgtaacgtc atcacaactg ttgcaatccc
     5341 attgatgatt cctagtatca cagctagcca aatgtctttt gcataggcag taaggtaagg
     5401 aatgaatcgg cggagcgtct gcaaggataa tttttttact ttcataacta ttcttcctcc
     5461 tgtgatgcga cgatttcctg atattctgct gattgagcaa gtaattcttc atgggtaccc
     5521 tgagcagcaa tcttaccttg atccatcact aagatatgat gtgcttcttg aatagagcgg
     5581 acacgttggg agataataat cactgtactg ccaagataag tctgtagcgc cttacgcaaa
     5641 tccaagtctg tttgataatc caacgcactt aaagagtcat ctaaaatcag taatgctggc
     5701 ttggcaatca aagctcttgc aatcgtcagt cgctgttttt gtccgccaga gaaatttttc
     5761 ccattttcca tgacttttgt atctaaacca tttggaagct gacggacaaa ttcttctgcc
     5821 tgagcgattt tcagagcttc ccaacattct ttttctgtgg cgtctttctt accccattga
     5881 aggttttcac gaatagtccc ggtaaatagt acagaagttt ggggaacaag agcaatttgc
     5941 tgacggagat gatccagctg ccaagaacga acatccattc cgtccattat aactgtaccg
     6001 gaactgacat catagaaacg cggaatcaat tgtgtcaaag tcgttttccc gcttccggtt
     6061 ggaccaatga ttccaaggac tgtatttcgc ttcagctgaa atgtaatatt ttggagagaa
     6121 agaccagaat caggagtata acggaagtca acatggtcaa aagcgataat tgcttcagaa
     6181 gaaaaatcgg gagtagtact gacctcagct tcaagaatcg aattttctgt gtctagcacc
     6241 tctttcaccc gatttcctga agcttcggca cgtgtgaaga tcacgacgag gtttgaaaca
     6301 acgatcagtg ccaaaagcat ttgattcata taattgatca aagcaagaac ttgtccttgt
     6361 tgcaagctac caatatttac tttgatcccg ccaactgtca gcaaacagat gatcccgaca
     6421 ttcatgatca aggttgtagc aggtgataat agggcagaga tattggaaac acgtaaataa
     6481 ttgttcgcta gatcatttgt gcttttatcg aacttctctg tttctttttt tgtctgcgca
     6541 aatgcgcgga tgacacgaac accgctaaga ttctgactaa cgtgttcatt gagctgatct
     6601 agtttttctt gcactttttg gtagagagga acggtttttt tgatgataaa aaataaaatg
     6661 attgagaaaa taggcaataa tgctaagaaa aacagaccta cttcccagtc gatcacaaaa
     6721 gccatgacca ctgacccgat acttaaaaac ggtgctctga tcaataaacg aatgaccatt
     6781 gctaaagcta gctgaagctg attgatgtcg tttgtcattc gggtgatcaa ggtatctgtt
     6841 ccaaaatgat tcaactcgtt gtgagagaaa gtattgattt ttttgattag ctgattacgt
     6901 aactctgtac caaatccttg tgaagctact gaagcatagt attggcaaat gatagcacaa
     6961 atcagaccaa tcacagacat agcgaccatc cataagccca tttcaatgat ttttcctcgg
     7021 tcattcattt ttagtccatt atcgatcaag gaagccatca ttaaaggcag gacaagttcg
     7081 aagacagctt caagaaattt gaagaacggg cccaaaatga tttgcttacg atagtctttt
     7141 gcataacgta ataactgaat catatgattc ctcctattat ttatttgtcc acaattgaaa
     7201 aaacaagtac taccacattg tagcttagga aatttagaat gaaaagagaa caagcctttt
     7261 cataaatgaa agttttatta tgaagaagct ttagaaattg aatagactat tttttttaaa
     7321 aaaaagaaaa t
//
LOCUS       DACEMO010000136         7146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEMO010000136 DACEMO010000000
VERSION     DACEMO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.141"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            299..1852
                     /locus_tag="IW728_002094"
     CDS             299..1852
                     /locus_tag="IW728_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1025437.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(2233..4017)
                     /locus_tag="IW728_002095"
     CDS             complement(2233..4017)
                     /locus_tag="IW728_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025438.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(4032..5753)
                     /locus_tag="IW728_002096"
     CDS             complement(4032..5753)
                     /locus_tag="IW728_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025439.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            5853..6614
                     /locus_tag="IW728_002097"
     CDS             5853..6614
                     /locus_tag="IW728_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1025440.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         2266 a         1258 c         1372 g         2250 t
ORIGIN      
        1 gccagaatga acttcattct gtctcagatt ttttgttatt tcaaattgat taattgattg
       61 atcaaaaagg ttttgatttt catgttctca aacacgttca atgtcttttt cttctgaatg
      121 gtcttgtaag agatctctgt aatcatcaaa tttttgtcta ttaatcttag tattttttga
      181 tgtataaact acctgaaatt gagagtacat accaaattca taaatttttt attagtggga
      241 ctagtgattg tatggatttt gacgcataat gaatgaagta tgagaaaagg agagacaaat
      301 ggatcaagaa ctatttatta tcgaagtatt atcagctaaa caaaaaatta tcgagaaagc
      361 gaaaaaaaga gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt tctctggttt
      421 gctttttttc ggagctgtat cgttcctata tcgccatatg agtgcagtag tccgttcagc
      481 tgaagggatg caaaaattaa ataatttgta tcacttttcg tggggagttg gcggaataac
      541 aattgttgtg cttattgttt ttcttctact tatttggctc cgttaccatc aattgtcgcg
      601 tcatttcgat caatggctta gtcaaagagc acaacagttt ttacataata aaccaacttc
      661 tcaagatgat gactatcttt attttgagaa gacttcgacg agtcaagctt ttcggataca
      721 aaaaagtgat tgccagttat tgctgagatc acttgatgat ttacctatct acttaggcga
      781 aagcaaatca aaattcggaa tgaacacatt ccatattttt ttactagaaa acgaacccag
      841 atgggaaaaa agccagtctt cgcctggtgg atacctgaca actaagatga gtacattagt
      901 cactggtttt ttcctcattc ttttagctgg aacaatctat tatgggagat acactcagcc
      961 aaaaatgtct tctcatataa atgctgcttc tcacatgaca atgaactcaa ctaacgatac
     1021 gcatatacaa aagacaaatg aagggttgtt agctgatcct aagcaaaagg taaatttgac
     1081 aacacaaaag ataaatgatc tcaaacttga tacagagacg cactctctct atatgacaac
     1141 agatagtggt gctaattggg tatttgtgcc gattgattcg caatggttgc gttttggcga
     1201 atacaccctg acaacggggc tgattcctga aggttattgg atggacaaca catatgatat
     1261 ttccgccgat ttctcatggt ttatctattc ttctggggat gatgtacgtc tgctgacttc
     1321 aagagataat ggtaaaacat ggcaaacgtc tattatcacc caatcagttc agccgatacg
     1381 ttatcgaaaa gcggtatttt atggcaacga tggtctattt atctgtacat cagccagtgg
     1441 gatgtctgca gaaactttgt acatttacac tactatggat ggcggaatca cttggataaa
     1501 aagtggaagt acaatgatca atcagccagt acaaaatgtc agcttcgtta caacttcttt
     1561 aggttttgtt tcgacacgtg aaagtttgtt ttatacaaac aacggaggac gctcattcaa
     1621 agaatcagtt ctgtcgattc cggaaaacta tcagacaggg gggatagatc tttttcaatc
     1681 accaaatgaa gttactcaaa taagcgcgaa tacgttagag acaaaatttt atttgttgaa
     1741 aaaaggacca atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa ctgataacgg
     1801 ggaaacatgg caattcgttc agcaactatc gcaagtagaa caaggagatt aaagaagaga
     1861 gattgtgagc ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa acagcttctc
     1921 atatttgttg aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa gaagcgattt
     1981 tgaagtatag cttctcgtat tttacaagat cgtgtcttat tgccgatagg ttgtctcttg
     2041 aacaccgttt attcgtaaaa agggattgtg acaagtcttt tgtcactact taccgaacga
     2101 aactgaagga gctatccaaa aagtatcatt agtattattc tttgtgtttc tagtggcacc
     2161 actagtacat ttaaaaaagc tatcgatcat ggactaatca tacatgatcg atagcttttt
     2221 agataatgtt tgttacgcta tctgaaattg actattataa agactggcat aaagcgttgg
     2281 tgattggagt agttcagaat gtgttccttt ttcgacgata tcgccattct tcattacaag
     2341 gatcatgtct gcattttcaa tcgttgacaa acggtgtgca atcacaaaac ttgtcctatc
     2401 ttttgtcaca gcttccattg cttgctgaat caatgcttct gttcgtgtat ctacgctaga
     2461 agttgcttca tctaaaataa cgacaggagg attcgctaga atgactcgag caattgtcaa
     2521 taattgttgc tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg tatcatatcc
     2581 gtctggcagt gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt cgataatttg
     2641 ttctcttgtg gcatccattt tcccgtaggc gatattatca gctaccgttc cttcaaacag
     2701 ccaggtattt tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg aaagcttggt
     2761 aatatcaaga ccgtcgaatt cgatctttcc tttattcagt tcataaaaac gcatcagcag
     2821 attgactaaa gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg tttgttttgg
     2881 ctttactgaa aaatcgactt gattcatcaa tattttatct gatgtgtaac caaactgtac
     2941 atttttgaaa gctatttttc ctttcggttg ttctaaaagt cttgcatctg gaaggtctgg
     3001 aatttcatct gtttcatcta atatctcaaa tatcctatcg attgctgcca ttgcggattg
     3061 gatcgagttg atcacataag aagcggtaga aattggttca gaaatctgat tgatgtactg
     3121 aagataagct tgtaagaagc cgatcgtcac accacctgat agaacaagca tagcaccgaa
     3181 taccgcactt atgatgaagg cgagttgatt aatgaaacga atggccggat agatagcaca
     3241 gttcataaat tgagcacgtt taaaagcgtt gtattgcttt ttatttactt cgtgtatagc
     3301 agcttctgca tgagcttgct gattgaatgt tttgaccact agattaccag ctaagatttc
     3361 ttccattcga ttatttaaaa cacccagttc atactgattc tgttcagaaa tctgtttgtt
     3421 tttgttcgca atcttagttg taacaattga actgccaacg atcagcaata agacgagcaa
     3481 agtaagcttg ctttcgatat aaaacagcat cagaccagca gcagctattg ttacgacaga
     3541 agtaaaaaat tgattgatac ctgtcaataa cacttgagaa agttgattga gaccggtagt
     3601 tgtccgacta atgatgtcac ctacttgatg attgtcaaaa aacgacatcg gtaatgtttt
     3661 gaattttttt gtgatttctt ttcgcaccct caatgtgatc ttttcgctta acgctgccat
     3721 tgtccgttcc tgaataaatg aagtgatgct gcttaagact gaacagccga tcagcaataa
     3781 gacagggaac aacaatgctt gtttgatatc actgaaagaa agctgtccaa tacctgtttt
     3841 tcttatttct gataagagat catcgatccc gattcccatg atcaagggca tggctacgac
     3901 taataggttc ccgaataaac tgcatagaaa caatccgtaa aaatatttat gttcaggttt
     3961 gatcattttc caaaaacgta tcaaagcacg cgtgttcaac gtttttttct tttccatcgt
     4021 atcccttctt tctatattcc ttgtgatttt gcaaactcct gataagtttg gcaattagac
     4081 agtagttctt catgagtacc ttgtccaacg atttttcctt catctaaaac aatgatgtta
     4141 tctgcttgtt ggattgtact gaggcgttga gcaacaatca cgagagtttt ctccgacatg
     4201 tgtttatgaa gggctgctct taatgctgca tctgttttat agtctaaagc ggagaaacta
     4261 tcatcgaata catagatctc tgctggtttg atcaacgctc gagcaataca tatccgctgt
     4321 ttctggcccc ctgaataatt agaaccgcct tgagcaacaa agctgtccaa tccgtcagga
     4381 agggtgtcca ctagttcttc taattgagcg atcttgatcg ctttagacaa ctcttctgtt
     4441 gaggcatatt catttcccat ctgcaagttg cttaaaattg tcccgctgaa taagaaagcc
     4501 ttctgaggga cataactgat gtgggagcgg atctgttctt gagtcatttg agtaagagga
     4561 ataccaccaa gggttacttt tcctatcgta gggtcactaa gtctcaagag aagtttcgcc
     4621 aaactacttt ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc tttaggtatt
     4681 gtgaaatcaa tatcttctag aactggatca gagatttcag tatatttaaa tgtgacatgt
     4741 tcaaaagcag ctgcatttgt ctgatcaaag gttggttcag ctactgtttt gatcgtctct
     4801 tcctcaattt cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga ggcaaacgat
     4861 ttagggataa tgaccaatac catagctgcg atcatcaagt aagcaagtgt cagcatagag
     4921 tattcgacta cagcagtcac gacaccgatt tgtaatgctc cttctgctac aagatttccg
     4981 ccaaaccaca aaatagaagc ataaacgatt cccatgagca aaaaagcaat tggagtgacc
     5041 caagcaaata ttcggttcac tttgatcatt tgattagcat agtcagcaaa gctcttattc
     5101 gttcgctttt cttcatatga ttgattatca aaggcacgga tcatattaat cccagtaaag
     5161 aattctctca atgtgatggt gatccgatcc atttttggct ggatactcaa agataaaggt
     5221 gcccctcgtt tcatcagtcg ataaacaatc catacataaa aaacaacaga gatcaatgta
     5281 acccatgtta gtaatagaga ataacgaaac atcatgagaa tggtgaaaac gcaaataacc
     5341 ggtgctggta agatcatttg gcaaaataaa acaatcattt gctggacatt atcgacatca
     5401 ttcgtcattc ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt cattgacagc
     5461 tgttggaatt tacggaaata tttctcccgc gtttggaatc caaacctagc ggaagccaat
     5521 gctgaaaagt aactagaggc aactgcagcg attgttccta gaaacgctac aagcagcatt
     5581 tcaactccaa ttttttttat aactgaggct tcgccacttg ctatacctgt gtcgatcagc
     5641 tttgctacta attttggtac acctaatgta ccggcgattt ctaaacagat catcacaaag
     5701 gtaaggagag cgagtccttt gtttttctgt aagaatgttt ttaagataga catattattc
     5761 ctcctttttt cctgatactt ttcgattcta taccttcaag taacttgaag gtcaatagaa
     5821 aaaagaataa actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg attggccaag
     5881 tatcggaact atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa
     5941 tccgttcaag tagacaagaa gagaatgatt atcgattgtt tgatatcgat gatattttta
     6001 tgctttatga tatcgttttt tatagaaaat tagatatacc gatcaaacag atgaagaatt
     6061 tatatggaaa aacattgacg gagttatatg agacacttga tgaaacagaa agaagaatcc
     6121 ataaagaact ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa aaaaaacagt
     6181 tgaaattgat gatcgataca aacgaggaag aatttcctgt agaagagatc ccttttgact
     6241 gtatgatctc aacggagttt gaagatattg tggaaatcaa aaaatttcta cctaactatt
     6301 caagctttgg gatgatgagt atgccagctt ccagttcaca gacgatgtat ggatttttta
     6361 tcgaccctag tgaagtacat ttatttactc aagacgtgat ttgggagaaa aaagatacgg
     6421 ctgtttatcg tcgattctta ttgaaatccg agatgaacca tgcggaacgt aataatattt
     6481 tagaaatcag agaaagaatg tatgaaaaag gatggaaaac aggagaagtg atcggccagt
     6541 atctattgac gaatacggat gaaaacaata tcagaactga atattatcat gcatggatag
     6601 aaatgaaaaa ataggaaata tgtgtttaag ataactcgaa aatgatggtt gttttagtta
     6661 taaagaaaaa ttttctggtt cgattacggg tttaatgtgg gggattatta gtcgtggata
     6721 gatagaattt tagtttttta gaggaaatat gtctttaaaa atggaatggt tgcaattctt
     6781 agcgatatat acgatgtaat tgtcctctta aaaaagtatg gaaaaataat tgtattgggt
     6841 gttatgtata attcatggtg aactgagact aatgagaaga aaaattcata ttgttatgag
     6901 tgagattgta tcagatgaga atgataaatt aaataggtgc ataaaagcat tacacttaaa
     6961 atacattgtt gcttacatta ttacaatatg ttaataaggc aaaaataaat agatggattg
     7021 aataaacata ttttttaatc aaatagtccc tactgtgact ttcctcagct atcggtgata
     7081 ctaccgatag catttttttg cttataaaat aattataagt atttagagat ttttcttcta
     7141 acattc
//
LOCUS       DACEMO010000137         7123 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEMO010000137 DACEMO010000000
VERSION     DACEMO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.142"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..325
                     /gene="sgrA"
                     /locus_tag="IW728_002098"
     CDS             <1..325
                     /gene="sgrA"
                     /locus_tag="IW728_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1025441.1"
                     /translation="SSESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSV
                     LKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSL
                     FHRVK"
     gene            623..979
                     /locus_tag="IW728_002099"
     CDS             623..979
                     /locus_tag="IW728_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025442.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            982..2139
                     /locus_tag="IW728_002100"
     CDS             982..2139
                     /locus_tag="IW728_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025443.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(2165..2605)
                     /locus_tag="IW728_002101"
     CDS             complement(2165..2605)
                     /locus_tag="IW728_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1025444.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2765..3271
                     /locus_tag="IW728_002102"
     CDS             2765..3271
                     /locus_tag="IW728_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1025445.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3439..5352
                     /locus_tag="IW728_002103"
     CDS             3439..5352
                     /locus_tag="IW728_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1025446.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5357..6358
                     /locus_tag="IW728_002104"
     CDS             5357..6358
                     /locus_tag="IW728_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1025447.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6560..6766)
                     /locus_tag="IW728_002105"
     CDS             complement(6560..6766)
                     /locus_tag="IW728_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1025448.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2640 a         1072 c         1412 g         1999 t
ORIGIN      
        1 gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa aagtaaataa
       61 tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg cagggagcaa
      121 cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc atcaagaaaa
      181 agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta gaaccttgaa
      241 caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa gctattttcg
      301 ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat tgaataaaac
      361 gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg aagttgtagc
      421 tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa caggaacaca
      481 aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag tatctctgaa
      541 aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa gtctaaataa
      601 tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg aaaggcaagt
      661 atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg tgaaggatat
      721 cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac aagcagaatc
      781 gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca agatgataaa
      841 agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt tagagggaac
      901 gattggttat attttactgg attcacttta caaaggtact tatcaatcct tagaaaatct
      961 tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga acttaatact
     1021 attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa
     1081 gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga cgaaaatcat
     1141 tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat
     1201 gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag aatttcgttg
     1261 caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa caaatttgtg
     1321 catccattga taacaaaaga agagtttacg cattacatac tagagccttt ttttgaagca
     1381 aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta ttttatgttt
     1441 ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa agtacaagaa
     1501 agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt caagatttgt
     1561 tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca tttgtattca
     1621 agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga agacagagat
     1681 tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag tggggtagct
     1741 cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat ttttcaatac
     1801 tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt ctatattcaa
     1861 tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc tatgactgaa
     1921 cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat tagcgattat
     1981 cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg gaatggacaa
     2041 cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa
     2101 aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat tgtttatgcc
     2161 aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga ccagaaagca
     2221 agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg attttatctt
     2281 cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt ttcaataaag
     2341 aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct attctgtgaa
     2401 caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg ttgtttgtca
     2461 attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag ttctgttcga
     2521 taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac gagcagccag
     2581 aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt atgaaataat
     2641 aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa atgttgtcac
     2701 gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga aagaaggcac
     2761 aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag gggtatgttt
     2821 tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata aaagaatcaa
     2881 aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg ctcaattagt
     2941 gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc acagttattt
     3001 tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt tattaactga
     3061 attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta tagatacgac
     3121 tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag caacgacaac
     3181 ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga aaatttggga
     3241 taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa aaagcagaat
     3301 ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc ctgttctatt
     3361 tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca tataattctc
     3421 ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa gggagcgttg
     3481 gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg agagagcatt
     3541 tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc tggaaatgaa
     3601 acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga gacatctgtt
     3661 actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga agaagcaaca
     3721 gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa agagaatggg
     3781 acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata tttgaattac
     3841 ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa gtcaaaatta
     3901 atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa agagattatc
     3961 aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg
     4021 atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac aggcttctat
     4081 ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa aatgatggat
     4141 gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca tggcggattt
     4201 cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta taaggccggg
     4261 tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga ttatgtgacc
     4321 tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt tcgagagcgg
     4381 ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat gatgtctgta
     4441 ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg atttgcatca
     4501 attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact accggatgcc
     4561 ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt tatgggcctt
     4621 ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc tctacctacg
     4681 attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga tgatgtagta
     4741 cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga tcttttatta
     4801 gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta cacgaaacat
     4861 gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta tgtggatgaa
     4921 aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc ttatggagaa
     4981 aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg
     5041 actgaaggga tttttaatga gacgatacca actattattt tctattacaa aaaaatccca
     5101 gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg aaagaatgaa
     5161 cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt gaaagtacaa
     5221 aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa acattcgctt
     5281 ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat ttggcgaaga
     5341 aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac cagttatcat
     5401 cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc cgattagctt
     5461 gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc gaccagatga
     5521 tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc cttcaaagta
     5581 caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat taccgattct
     5641 tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc gtgattattt
     5701 ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg aacgatcacc
     5761 ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag cagctaaaaa
     5821 agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc
     5881 acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa aagctgatca
     5941 tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt ctggtctgtt
     6001 ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt atgaaaaatg
     6061 tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca aaagtttagc
     6121 cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg gaaatacaaa
     6181 tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt
     6241 cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc agtttgattt
     6301 cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca aggagtaaag
     6361 tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa ggagcaattt
     6421 caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg agttagagat
     6481 ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt cacgatctct
     6541 tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc
     6601 cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag aatgctggtt
     6661 ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata gcaaataaaa
     6721 tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc agcacctcct
     6781 tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt ttgttaggat
     6841 gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata agcaattaat
     6901 gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa taatagaaaa
     6961 tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc aaaaattcga
     7021 tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat aaaattacaa
     7081 atgataacct aaaatgaggc caaaagccag tgttatcaag gaa
//
LOCUS       DACEMO010000138         7052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEMO010000138 DACEMO010000000
VERSION     DACEMO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.143"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            163..1104
                     /locus_tag="IW728_002106"
     CDS             163..1104
                     /locus_tag="IW728_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1025449.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1173..1904
                     /locus_tag="IW728_002107"
     CDS             1173..1904
                     /locus_tag="IW728_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1025450.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2048..4228
                     /locus_tag="IW728_002108"
     CDS             2048..4228
                     /locus_tag="IW728_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1025451.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4248..4730
                     /locus_tag="IW728_002109"
     CDS             4248..4730
                     /locus_tag="IW728_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1025452.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4746..5507
                     /locus_tag="IW728_002110"
     CDS             4746..5507
                     /locus_tag="IW728_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1025453.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5638..6972
                     /locus_tag="IW728_002111"
     CDS             5638..6972
                     /locus_tag="IW728_002111"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1025454.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT         2369 a         1239 c         1553 g         1891 t
ORIGIN      
        1 tttttctgtg cgaaacagga aagcgaagtc tttttaattc aaataataat attatgtaaa
       61 caaaaaactt tccatgcgta attatacaca agcaaataaa tcgtcaaatg cgaacgcctt
      121 ctttatactg tttaggaacg aaaagaagga aggaagaaat acatggatta tcaagtactg
      181 ctttattaca attatacaaa aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc
      241 ttttgcaaat ctttggatct taaaggtcgt attctagtag ctgaagaagg aataaatggt
      301 actgtgtctg gaaccgttga aaacacgact gcctatatga aagcaatgaa agctgatcct
      361 cgatttgctg atacgatgtt caagatcgat ccaagcgaag gacatgcctt taagaagatg
      421 tttgtaagat accgtccaga gcttgtaagt ttaaagctag aagaggacat tgatccaaac
      481 caagtgacag gtgcctatct ttcaccgcaa gaatttaaag aagctatttt agatgaacag
      541 acagtggtga ttgatgcacg gaatgattat gaatatgact tgggacattt tcgaggggct
      601 gtacgcccag atatacgcag ttttcgagag cttccagact ggatccgcga gcacaaagaa
      661 gaatttatgg aaaaacgggt agtgacctac tgtactggtg gcattcgttg cgagaagttt
      721 tctggttggc ttgtccgtga aggattcaaa gatgtcggac agctgcatgg cggtattgct
      781 acttatggta aggatcctga agtccaagga gaattatggg acggcaagat gtatgtgttt
      841 gatgaacgga tcgctgtcga tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg
      901 tttgacggaa caccatgtga acgatatatc aattgtagca atcctgagtg caatcgtcag
      961 attcttgctt ccattgaaaa tgaagaaaaa catttaggag catgtagcat agagtgtgcg
     1021 aaacatccgc gcaatcgcta tgttcaagac catggaatct ctccagaaga agcagaactg
     1081 cggtttacag ctttaattaa atagaaaaca atagttttga ttgtcgacca tttccaatat
     1141 gcgctatact agcgttgagg atggtgatcc acatgaaatc attgatttat gctattgggg
     1201 atgtccatgg caaacatgag ctttttgaaa aacttattca ctattatgat ccaaagatcc
     1261 atcagcttgt tttattaggg gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt
     1321 tattaggaaa gcaattagtc gaagaaacag gagccattta cctccgggga aaccatgaag
     1381 aatatttttt gcaatttctg caatcaccgg aggactggta tgcgccttat atgagaaatg
     1441 gtggaaaaga aacgtttgaa agcctgattc acccaggggc atcggaggaa tactccccaa
     1501 ctgaaatctc gatgatgatc caatcgagat attcagaact gattaaattt ttagtccagc
     1561 gccccttgta ttatgaatgg gaaaattatc tttttgtcca tgccggtgtg gatttgacaa
     1621 agaaagactg gcgtaagaca agtctaagag actttatttg gataagggaa ccatttcatg
     1681 aagggaaaaa caatacaggt aagacaattg tatttggaca tacgattaca ccaatgctcc
     1741 atggagatat gcagacaaca gacctctgga ttcaggatca taaaatcgga atggacggtg
     1801 gggctgtatt cggagggtca ttgcatgggg ttgtttttga caaggaaggt attcttcaag
     1861 atattgaata tcagaacaac gaaggacctt ggcatggaat ctgactaatg gaaagaatga
     1921 aggagctgtg actttgttgc ggctcttttt atttttatag agaatattgt ttctttcagt
     1981 agtttaaccc aggataaaaa gtatgataga ataaagaagc aatttttagc agaaaagagg
     2041 cattttaatg acggaaaatt tgaaccagac gatcattcag cttgtacaaa aagaactttc
     2101 agactatcgt cctaagcagt tgactactgt acttaatttg ttaaatgaag gaaacactgt
     2161 tccttttatc gcgcgttacc gaaaagaaat gactggaagt cttgatgaag tccagattcg
     2221 tgaaatcgaa gagcggtatg cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat
     2281 tcggctaatc gatgaacaag ggaaactgac tcccgagtta gaagcagaaa tcacccaagc
     2341 cgtgaaaatg caacaagtgg aagatttgta tcgtccatac aaacaaaaac gacggacaaa
     2401 agcaacgatt gctaaagaaa aaggacttga accactcgcg aagtggctga tgcagcttac
     2461 agatggagag gtgcaatctg aagcagaaaa atatattgac aaagaaaaag aagtctcttc
     2521 tgccgaagaa gcgttgcacg gtgctcatga gattattgct gaacaagtca gcgataatgc
     2581 aaaatttcgg acttggattc ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt
     2641 caaggatgag caagcagatg aaaaaggcgt atatgaaatg tattatgact ttgcagaacc
     2701 cgtccataaa atggtttctc atcgaatatt agctactaat cgtggagaaa aagaagatat
     2761 tctgaaagta ttctttcaag tagatgaagc agcaatttta gcttacttgg atcgccaaat
     2821 agtcaaaaat cctgcctctc cttcttcttc attcgtcaga gaagcctatc aggatagtta
     2881 taaacgcttt attcagccag caatcgagcg agaactgcgc aatgaattga ctgaaaaggc
     2941 agatgagcaa gcaatcgcta ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc
     3001 actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat gtaaactagc
     3061 agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc ataaacctgc
     3121 agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta ataaatatca
     3181 agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac tttttgtggc
     3241 tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg aagcaggcgc
     3301 ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac aagttgaaga
     3361 aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat
     3421 cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa aacgtttggc
     3481 tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac
     3541 agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg cccaaaacat
     3601 tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga aaaaagtgcc
     3661 acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa
     3721 aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa aagaaatatt
     3781 aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag aaaaattgac
     3841 acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa cgttagccga
     3901 tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc cagcgccctt
     3961 attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat tgactggaac
     4021 ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc aagacggact
     4081 tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt
     4141 aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggca gaatcagttt
     4201 gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg acacaagaag
     4261 aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct ttcaagcacc
     4321 aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta aacgatcatc
     4381 atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt gcaggaatca
     4441 tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat cagcataaag
     4501 atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca ccagccttga
     4561 cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc acgatctgcc
     4621 gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat
     4681 ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga aaggatagaa
     4741 ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta ttaaattttt
     4801 tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt attttagctg
     4861 gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat ggccaagtag
     4921 atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat gagaattttg
     4981 aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc
     5041 taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag tctacaaatt
     5101 ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct gtaccagaaa
     5161 aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact tttgagaaag
     5221 tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg gaaatccttc
     5281 acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg cctaaggaat
     5341 atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag aacggctatg
     5401 gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt tggtcttctt
     5461 acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca ttttagcaag
     5521 cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa gataatgagg
     5581 agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag gattcaaatg
     5641 gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat gagctccttg
     5701 gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga aaaatatttc
     5761 tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa cgcagataat
     5821 gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca tgaagaaatc
     5881 cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat tggtcatttt
     5941 atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag tacgactgga
     6001 ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg agatggaaca
     6061 ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata tcgccgtcat
     6121 ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga tcatccggat
     6181 tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg acaagtgaaa
     6241 aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc aaatgtgccg
     6301 atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat cgaacgcaca
     6361 acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca cttcactgta
     6421 ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt agcttatttc
     6481 gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg agtaaaacgt
     6541 cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc gcatcaccct
     6601 gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa agaaataatt
     6661 gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga atttgcagaa
     6721 gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc tcgggaagaa
     6781 caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc
     6841 aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat cttcatgggt
     6901 gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa
     6961 aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagagg gggagtggca
     7021 aaaatcttgc caagccccct tttttgcaac tt
//
LOCUS       DACEMO010000139         6976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEMO010000139 DACEMO010000000
VERSION     DACEMO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.144"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            160..633
                     /locus_tag="IW728_002112"
     CDS             160..633
                     /locus_tag="IW728_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1025455.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            630..2762
                     /gene="feoB"
                     /locus_tag="IW728_002113"
     CDS             630..2762
                     /gene="feoB"
                     /locus_tag="IW728_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1025456.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2764..2898
                     /locus_tag="IW728_002114"
     CDS             2764..2898
                     /locus_tag="IW728_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1025457.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2992..3636
                     /locus_tag="IW728_002115"
     CDS             2992..3636
                     /locus_tag="IW728_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1025458.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3823..5016
                     /locus_tag="IW728_002116"
     CDS             3823..5016
                     /locus_tag="IW728_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1025459.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5009..6736
                     /locus_tag="IW728_002117"
     CDS             5009..6736
                     /locus_tag="IW728_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1025460.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2248 a         1230 c         1561 g         1937 t
ORIGIN      
        1 aagattcgct cataatatca caagtataat tgacattctc aattaccgag agtattctat
       61 gtgagaatga ttctcattta tttgcgtaac acgtaagaaa attagaacat ataatgaatg
      121 attttaatac atgatttatt tcacaagaga ggaatcaata tgcagacctt agcgcaggca
      181 aagcagaatt tcgtttatca agtcgaacga atcgaaacag aagaaaaaac aaaacgccat
      241 ttgcaaaata tcgggatcgt tgccggaagt aaaatcgtcg tcgtcaattt atcaggcaac
      301 aatgccattt tactggtaaa aagtcatcga gtggcagtaa gtcgggaaat actaggtcaa
      361 atctttgtca ataaaaagac tgcttcctct gaaagctgga cttctcttga tcaattaaaa
      421 gcaggagaga ctgggatcgt cgtagcaatc cacggacaag gcgcagtgaa gcgacggtta
      481 atggatatgg gattgacgcg aaatgtaaaa atagttatcc gaaaactagc ccctctggga
      541 gatccaatcg aactgacagt gcgagggtat gaactgacgt tacgaaagtc agaggccgaa
      601 ctaatcttga tacaaaggga ggaaaaagaa tgaatatcgc gttagtcgga aatccaaaca
      661 gcgggaaaac tagcttgttc aatctcttga ctggttcgaa tcaatatgta gggaattggc
      721 cgggcgtaac agtagaaaaa aaagaaggat cgttgaaaaa aaatccgaac attcatattc
      781 aggacctacc cggcatctat tctttgtctc cttatacacc agaagaagtg attgcaagag
      841 attatttggt tcaggaacag cctgaactga tcatcaacat tattgatgct accaatttag
      901 aacgaaatct ttatttaact acgcagttat tggatttaga cattcctatg atcattggtc
      961 tgaatatgat ggatgtcatc aaaaaagaag gaaaaaaaat caatctagaa aagctttcct
     1021 atggtcttgg tgtaccagtt ttaccaatca gcgtattgaa aaagcaagga atcgaaaaac
     1081 taatcgaaaa gattcaggag tctgcaaaaa agcggccttc tattcctgtt ccctgtacat
     1141 atgatccacg tttagaagca gctttacatg aaattgaaga tgtactagga gaacaaagcg
     1201 gcaagaaacg ctggtatgca atgaaatatt ttgaacgaga tcagaaggta agagaggatt
     1261 acgagatagc agtttctcag caaaaagaga tcgagcaact gatccagtta acagagaaac
     1321 tattggacga cgatagtgag acgattctgg tcaatgaacg ttatgaattt attacccaac
     1381 tatgtacact ctgtgttgtg tcaaatgatt ctttccaatt atcgatgagt gacaaaatcg
     1441 atcagattgc aacgaatcgt tggttagcct tgccgatctt cgcatttgtg atgtggctga
     1501 tctattatct ggctattcaa acagtaggaa caatggggac agattggatc aatgacacat
     1561 tgtttggtac atggttacca gaacatgttg gcagattgtt agctgaatgg caagtagcgg
     1621 gatggatgca ggagctgatc ttgaacggca tcatcgcagg cgtgggagca gtgttaggat
     1681 ttctaccgca attgattata ttgtttttat gcttgagctt tctggaagac tgcggatata
     1741 tggcgaggat cgcttttgtc atggatcgac ttttcaggaa attcggtctt tcaggaaaat
     1801 ccttcattcc tatgctgatt gctacaggat gtggagtgcc aggcgtcatg gccagtcgca
     1861 cgattgaaaa cgagcaagat cgtcgtttga cgatcatggt cacaaccttc atgccttgtt
     1921 ctgcaaagtt gccgattatt gccttagtag caggggcctt ttttcctcat caatcttggg
     1981 tagctccttc tgcctatttt cttggtatga ctgcaattat tttctctgga atcagcttga
     2041 aaaaaacacg tttgtttgct ggagatacag ctccttttat catggaattg cccgcatatc
     2101 attttcctca atggtcagcg gtcctaagac aaacctatga ccgcagcaag tcttttgtta
     2161 aaaaagcagg aacgattatc tttgtttcta gtatcgtgat ctggttcagc tcttcttata
     2221 atttttatgc tcaacctgtt tctgaagacc aaagtatctt agcctttttc gggcgaatcc
     2281 tggcagttgt ttttcagccg cttggttggg gaaactggca aggaacagtc gcagcgatta
     2341 caggattggt cgctaaagaa aatattattg gcacatttgg tattttgttt ggtcatgcgg
     2401 aagtatccga aaatggcaga gaaatctggc aagtgctaca acacacatat actcccgcag
     2461 cagcttattc attgttagcc ttcaatttac tttgtgcgcc ttgttttgcg gcaattgggg
     2521 ctatccatcg ggaaatgaat gccaagaaat ggacttggat cgccataggc tatcaatgcg
     2581 gtttagctta tttagttagt tttgtgattt atcagttggg acacttattg gagggtggac
     2641 aggtagcgtt agggacgtat cttgctattt tactgatgat aggactcgtt tatgttttgt
     2701 tccgtcagcc aaaatccaag aaccagtttt atactatatt gtcattagaa ggagaggaat
     2761 aggatggcga cattcttatt aagtgtgtta atctttggaa gtgcaggcgc aattgtctat
     2821 actcgtttaa aatccggtaa gaattgtgat gactgtcaaa cagcttgccc agtaaaaaag
     2881 gaacagatca agcgatgatc agtcgttgca agaggctatt ctcccctttt ttgcttattt
     2941 tgctagagca tagtaagatg agggaaagta aaaaaagaaa gaggaaagaa aatgtctgga
     3001 aagaaaaaaa tcgtccgccc gcgctaccag cagattgctg ttgatttagc cgaacgtatc
     3061 gttgaaaacc gatataaagt gggtgagaag atccatgccc gctcaacact tgccagtacg
     3121 tacaatgctt ctcctgagac tacccgcaag gctatcaatg tgctggtaga tcttggaatc
     3181 atggaagtac ggcatggaag tggggcattt gttgcttcga aggaaaaagc aaaagatttc
     3241 ctattgcaat atcaagatgt acaatccatc caagatacaa aagcagaaat catggcaagt
     3301 gtggagaagc aacaagatga gctgaatcat ttttctcaat tattggatca gctggtcaat
     3361 caaacgaagc gaatacataa gatgaatcct ttacttccgt ttgaattatc tttatcagaa
     3421 aaagcaatgc atctggaccg ttcaattagc gagttgaata tctggcaggc gactggcgcc
     3481 acagttatcg caatttctca taatgaagaa ctgcttgttt cacctggccc ctatgctaaa
     3541 tttacggcag gggacatcgt tcattttatt ggaaatgaat atacactaca gcgtatgaca
     3601 aacttttttt acccggagca tcagcttgca gaatgattgt gtgaaatatt aaaaaaatat
     3661 taacttggct aaacctttta tttacaaaga tttagccaag tttttttcag tttggacaag
     3721 caaatttctt tcatttttga caaagtgact acatgatgtt aacatagtgt ggtcactttt
     3781 ttcagaaaga aaaatgaatt tacataaaaa aggagaattt gtttggtaaa agtagaagta
     3841 aaacatttaa caaagatatt tggtaaaaag acacaagccg ctttagatat gatgaatgat
     3901 caccagccga aaacggagat tttaaagaaa accggtgcaa cggttggtgt gtatgacgtg
     3961 aattttgacg taaaagaagg agaaatcttc gtgatcatgg ggctttccgg gagtgggaaa
     4021 tccacattga tccgtctatt gaatcgtctg attgagccaa cttcgggcag tatctatatt
     4081 gatggagaag atgtatcgaa gttaagcaaa gaagaattgc gagaagtccg tcgtcataaa
     4141 atcaatatgg ttttccaaaa cttcggtctg tttccacatc gaacgatctt ggaaaataca
     4201 gaatacgggt tagaagtccg tggtgtacct aaagaagaac gacaagaaaa agcagaaaaa
     4261 gcattagaaa attctagttt gctttcattt aaagaccaat atcctagtca gttatctggt
     4321 gggatgcaac aacgggttgg cctagccaga gctttagcaa atgatcctga gatcttgctg
     4381 atggacgaag cgttctcggc tcttgatccg ctgatccggc gagaaatgca agacgaatta
     4441 ctggacctgc aagccaatgt tcaaaaaacg attattttca ttacccatga tttgaatgaa
     4501 gctttacgga ttggggacag aatcgccttg atgaaagacg gcgaaatcat gcagatcgga
     4561 acaggtgaag aaatattgac caatccagca aatgattatg tacgtgaatt tgtggaagaa
     4621 gtcgatcgtt ccaaagtgct tacggctcaa aacatcatgg tacctgcatt gacgacgaat
     4681 atcgaatctg atggaccaaa cgttgcattg acaaggatgc gtaatgaaga agtcagcatg
     4741 ctgatggctg ttgacagaaa gagacacctg aaagggatca tcactgcaga tcaagcgtta
     4801 gaagctagga agcaaaaacg tccattgatc gacttcctag atgaaaatgt gacggtgatt
     4861 gggaaagaca tggtggtaag tgatattttc aatattattt atgactcacc gactccactt
     4921 gcagttgtag aggatggaaa attaaaaggt gtcgtcatcc gaggtagtgt gattgaagca
     4981 ttagcagaaa caagcgaggt gagtgaacat gaataatcta ttgaactatc aattaccagt
     5041 ggcaaactgg gtagaaaata tcacagaatg gtttacgaca actttttctg gcctattttc
     5101 atttttacaa acgattggac aggcagtgat gtcggggatt acaaatttac tgttagtgat
     5161 tccagctcct ttgtttatct tgttattgac ggtagcagct ttctttattt ctaagaaacg
     5221 tccaggactt acgttgttca cattaatcgg tctatggttt atttataacc aaggtctatg
     5281 gaatgatttg atgaatactg tcacacttgt tcttttatct agtgtgatct cgatcatcat
     5341 tggggtacca ttagggatct tgatggcaaa aagcagtaaa gcccaaagta ttatcaaacc
     5401 gatccttgac tttatgcaga caatgccagg attcgtttat ttgattccag cagttgcatt
     5461 tttcggtatc gggatggttc caggggtatt tgcctcagtc atctttgctc tgccaccaac
     5521 cgttcgtttt acgaatttag ggattcgaca agttcctaaa gaattagtag aagcttctga
     5581 ttcattcggg agcacaagtc gtcaaaaact attcaaacta gaattgcctt tagctaaaag
     5641 tacgatcatg gcagggatca atcaaacaac aatgctgtca ctttcaatgg tcgttattgc
     5701 ctctatgatc ggtgcacccg gcttaggacg cggcgtatta tcagcattgc aaagagcaca
     5761 agtcgggaat ggatttgtca acggggttgc attagttatc ttagcaatca tcattgaccg
     5821 ttttacacag catttgaacc agccaaacaa taaaaaagct gcaggagcaa caacacagaa
     5881 aagtaaaaaa cgtcaaggac tgatcattgg tgctgtagta gtagtgatct taggtgcgat
     5941 tggtataggg agtttttcat ctgccaaaga aacaaaacgt atcaacttat cttatgttga
     6001 atgggataca gaagttgctt caacaaatgt agtcggtgaa gtattgaaac aaatgggcta
     6061 tgatgtaacg atgacgccat tagataattc aatcatgtgg aaatctgttt caaatggcga
     6121 gtcagatgcc atggtttcag cttggctacc taaaactcat ggttcacaat atgcacaata
     6181 taaagatcaa gtcgaagacc taggtgccaa tttgacagga gcaaaagttg gtttagcagt
     6241 gccagcctac atggatgtaa attcgatcga tgagttaacc gatcaggcag gtaagaaaat
     6301 cataggaatc gagccaggag ctggtgtagt tacggctgct gaaaacacga tccaaaaata
     6361 tgacaacttg aaagactgga aagtagagac atcttcttca ggagcaatga ccgttgcact
     6421 aggtcaagcg atcaaaaaac acgaaccaat tgtagttaca ggatggacac ctcattggat
     6481 gtttgccaaa tatgatctga aatacttaga agatcctgaa aacggaatgg gaagcgaaga
     6541 gcagatccat acgatggttc gaaaaggatt gaaagaagac cagccagaag cttacaaagt
     6601 attagacaat ttccattggt cagaaaaaga tatggaaaaa gttatgttgg aaatcaacaa
     6661 tggaaaagat ccacagcaag cagcaaaaga ctggatcaaa gagaaccaag aattagtcga
     6721 aagctggaaa aaataaaaag acagacacga aataaatgga gcttccaaaa ttggatcaaa
     6781 tatccaaatt tggaagctcc atgttcgtct ctatcttttt tgtctataaa aaaagagaat
     6841 gcgtagctca tcttcgaaac gttcaaagac gccgacaaac tattatgaat ttatgggtgc
     6901 gagtcaaaga gatcgatcgt aggtatgaaa gactaatgtg attaaagttt attatgtacg
     6961 aagtgcattc tcaaaa
//
LOCUS       DACEMO010000140         6912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEMO010000140 DACEMO010000000
VERSION     DACEMO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.145"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            99..746
                     /locus_tag="IW728_002118"
     CDS             99..746
                     /locus_tag="IW728_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1025461.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IW728_002119"
     CDS             1139..3784
                     /locus_tag="IW728_002119"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1025462.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IW728_002120"
     CDS             4094..5407
                     /locus_tag="IW728_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1025463.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IW728_002121"
     CDS             5397..6050
                     /locus_tag="IW728_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1025464.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="IW728_002122"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="IW728_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ1025465.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
BASE COUNT         2264 a         1178 c         1483 g         1987 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tcctctaagt
     6901 ctataacttc at
//
LOCUS       DACEMO010000141         6905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEMO010000141 DACEMO010000000
VERSION     DACEMO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.146"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            99..389
                     /locus_tag="IW728_002123"
     CDS             99..389
                     /locus_tag="IW728_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025466.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            402..1427
                     /gene="sppA"
                     /locus_tag="IW728_002124"
     CDS             402..1427
                     /gene="sppA"
                     /locus_tag="IW728_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1025467.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            1430..2089
                     /locus_tag="IW728_002125"
     CDS             1430..2089
                     /locus_tag="IW728_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1025468.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            2246..3178
                     /locus_tag="IW728_002126"
     CDS             2246..3178
                     /locus_tag="IW728_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025469.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            3332..4621
                     /locus_tag="IW728_002127"
     CDS             3332..4621
                     /locus_tag="IW728_002127"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1025470.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            4868..6118
                     /gene="clpX"
                     /locus_tag="IW728_002128"
     CDS             4868..6118
                     /gene="clpX"
                     /locus_tag="IW728_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1025471.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            6201..6788
                     /locus_tag="IW728_002129"
     CDS             6201..6788
                     /locus_tag="IW728_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1025472.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         2397 a         1129 c         1511 g         1868 t
ORIGIN      
        1 aaatgtcctt tttatgtcaa aaaataatgg gtttttatct atcatctttt tcaggagttc
       61 ggtacaatag aaagtaatcg gatagaggag gagtaaaaat ggattcaaaa attgagatca
      121 tgacactggg aatgttaaaa aaacaattgt ccaaattcga agcatcagct ggtgtatcag
      181 atgatacgaa aatattttta gatactggct gggatagcat ccaagaaatc gcacctgatg
      241 ccttggaagt tgctcaagct cgtgagttta cggtagaaga tgaattaaca aaagagtcgt
      301 tttccgggta tgcgcgggaa gaaaaagcag agcggttcga cacttctgag cagtcagaaa
      361 ctgtgatcgt cataaaaaac ttgtattaga aaggagaaag aatgaataaa agacgttggg
      421 cagcagttct gattgctgca ggattatttg tggtttcgtt agtttcagct agtttgacta
      481 agcctcagga aaacgatcaa attgagggat tgaacagttg gttatatggg gatgaagaac
      541 tagctccaaa tgtgttggaa gagggagaca gtacgcaaaa aatcgtaaaa ttatcagtag
      601 atggaacgat tgctgcggga gggtcttcag gtttatttgc ttctgaaggt tacaatcata
      661 aaaattttat ggaacaactg aaggcgattg aagaagatcc aacagtcaaa ggtatctttt
      721 tagaagtaaa ttctcctggt ggcggtgtct acgaaagtgc ggaaatcgct aagaaattgg
      781 atacgattcg taaagaacat gatatcccaa tgtatgtcag catgaaaaac atggcagcta
      841 gcggtggcta ctatatttct gctcaagcag ataaaatctt tgcaacagaa gaaacagtga
      901 ccggctcaat tggtgtgatc atgtcaggat tgaattattc aggacttcta gaaaaactgg
      961 gtgtggaaga tacaacagtt aaaagcggag cgttgaaaga tatgggttca ggaacgcgac
     1021 cagagacaaa agaagaagaa gcggtgcttc aagcatatat cgacagcgca tatagtcgtt
     1081 ttgtagcaat cgtcagcgag ggacggaata aatctgaaga agaagtcaaa aaaattgctg
     1141 atggaaggat ttatgatggt gtccaagcaa aagaagtagg tttagtcgat gaattgggat
     1201 ttcctgaaga tgctttaaaa gctatacgaa aggaacaaca gcttgaagat gcggaactag
     1261 tagaatattc ttccacgcca tctgggtttg ctagtacatg gttaggaagt aaactagcag
     1321 aattccaggg attgaagcct tctgagacta gtcagatctt atccgttctt gagaatctag
     1381 ggacagcgga atcaccaaaa gcaatgtatt attacggagg tgagtaagca tgtcagagct
     1441 ttcaaatgaa aataaggaac aagaaaaaga taagcaacag cagcctgctg taggacctga
     1501 ctccaacttt tcccaaaagg tactgagctc ttttgaaaga gagcctttga cgcccgaaga
     1561 aatcaaaaag aaacaacgta aatggaaaaa gtatacggac acaagaaatg aaccgccaaa
     1621 agcaaatgat tttcctaagt atttctatgc aggttttttt atccgaatgt ttgccttctt
     1681 ggtcgatctt ttgtgtatcg gagctattac gcgtttaaca cttggtattg ccaaaaatct
     1741 aggttggctt aatttttcag attcttatct gagtctctac ggattcacag ccttacttat
     1801 ttatctagcg tattttattt tattgacgaa attcaatcat ggccaaacga ttgggaaaat
     1861 gatctttggt attcgagtca tcagctttga tgaagaagaa cttagctggt caaccgtttt
     1921 agttagagaa ggtgcttgcc ggttcattct aaaatttcct ttgctgatgt tagggtatct
     1981 tccagcagtc tttaatcaga aaaagcaaca tctgggcgat tttttcacta atacaagtgt
     2041 tgttacgatc aatctgatca aagcgttcaa ccaggaagtc aacagctgat gagcaataaa
     2101 aagcagtaac agttgaaagc accttctaaa agtcagatct aaaatctagc ttttagaggg
     2161 tgcttcagtt atttttaatt gtcatgcttt ttttgttttg ctactattga acatgagctg
     2221 aatatgagca agaggaggac actgcgtgaa gccgattgat tttccagaaa aatatgagaa
     2281 tttaatgcga gtggcacaac aagcactagc aaaccagcaa tatcagcagg caaaagaatt
     2341 atttcaacga gcttacgagt tgaaagaaag ctttgaggct aatagcctgc ttgttttttg
     2401 tctttacgag ttggacgaga aaaaagaagc attgaagcaa gcactcttgc atgaaaaaca
     2461 gtatcttcaa aatgaggagt ttgctgaatt ttattttgac ttgttaatca gtacacaaga
     2521 ttttttatat gctagaaaat tgatagcaag tacagatttt tatgaagctt tcgagcagcg
     2581 ggtcattgaa aaaatccaat ttgcagaaga attatccgga caaatggaga ggcagaagat
     2641 caaggcactc cacaataaat cggaggaact tccttcgttg gaacctgcac gtcagcttgc
     2701 gtctattgat gagatcgaac agcttcctta tcatgagttt atccaaacag cgagtaaact
     2761 tattgtatcg ccagaggtcc atattttagc aagggcaaaa ttactggaga cacttaggca
     2821 attgaacgaa tgccatcctg ttttctattt gacgattgaa gaaaagttag tcaaagtcat
     2881 accaaaagat ctgccaaagc cgcagcagca ggcaagttac cagcaattat gtgtattttc
     2941 agatcgttac gggaatgaag atgcgctgct tagttccgtg ttaaaagaag aattcacgtt
     3001 gcaatctgca attgtttatc ctgtctatga tacatacatc gaagacccga agcgctggtt
     3061 tcaactgact gtagaggcgt gtacaggaaa aacgatgtat gaaggtacag aagatgaaaa
     3121 acaagatttt ttaaaaaaaa gagaaaaaat tttgcagcaa atgatcttct tccattagat
     3181 tttatcagtg aaaaagataa aaatgttatg aaaaacaagg attctatcca ttcaatatgt
     3241 ttacaatttg aaagggttag tgtactattt aaaggtatgc atgaaacgtt tttgatgtat
     3301 aaaatgaatt tctaaactcg gagggaatta aatgactgca acatttgaaa aaaaaggcac
     3361 gaatgatggt gttttaaaat ttacgattgc gcaagacgaa atccaaaaag gattaacaac
     3421 tgcgtttaat aaagtgaaaa aaagcttgaa cgtacctgga ttccgtaaag ggaaagtttc
     3481 tcgtcaagta ttcaaccgta tgtacggtga agaagcttta tatgaagatg cattgaatgc
     3541 tgtattacca gaagcttatg aagctgctgt caaagaagct ggcatcgatc ctgtttctca
     3601 accaaaaatc gatgttgaaa gcatggaaaa aggtcaagac tgggtaatca ctgctgaagt
     3661 aacagtgaaa ccagaagtaa aattaggaca atataaagat ctagttgttg aaaaacaaga
     3721 ccgtgaagta acagatgctg acgtggatgc acgtatcaaa cgtgaacaag aagcacaagc
     3781 tgaattagtt atcaaagaag atgctgcagc tgaaaatggc gatacagtag taatcgactt
     3841 cgaaggcttt gtagatggcg aagcgtttga tggcggaaaa ggcgaaaatt actcacttga
     3901 attaggttca ggctcattca ttccaggttt tgaagatcaa ttagttggtc ataaatcagg
     3961 tgaaagtcta gatgtaaatg taactttccc agaagattat caagcagaag atcttgctgg
     4021 aaaagaagca gtcttcaaag tgacaatcca tgaagtaaaa gcaaaagaag tgcctgaatt
     4081 agatgacgaa ttcgcaaaag atgttgatga cgaagtatca acattagacg aattgaaaga
     4141 aaaatatcgc aaagaattag tagaaacaaa agaaaaagca gcggacgatg caaaagatga
     4201 agcagcgatc cgtatggctg ttgaaaacgc agaaatcgtt gaattgccac acgtgatggt
     4261 tcatgatgaa gtacatcgtt caatggacga attcttgaac aacatgcaac gtcaaggtat
     4321 ctctcctgaa atgtactatc aattaactgg ttctacagaa gaagacttgc ataaacaatt
     4381 cgaaggtgaa gcagaaatgc gcacaagaac aaatcttgtg atcgaagcaa ttgcagctgc
     4441 tgaaaatctt caagcaacag aagaagaaat caatgcagaa atccaagaat tatcagaaac
     4501 atacaacatg ccaatcgaac aaatcaaacg cgtgttgaca gaagatatgc tggaacatga
     4561 tgtgacaatg aaaaaagcaa tcgaattgat cacaggtaca gcgacagaag cgaaagaata
     4621 gaaatcaagt aataaatcat ttctgaagaa gctacttcta gaaaattgtt tattcgaaat
     4681 aagggactgt gacacgattt tgtcacagtt ccttattttt tgttaagagt aaaaaatgca
     4741 aaggtatagg tcagttgatc ctttgttaaa cactttttct ttttactaaa ctatgttatg
     4801 atagtaaaag actgtaaagt ctttgatagt ttcttgcata ctttttgcaa gttaaggggt
     4861 gaagaccatg tatgacaatc caagtagcaa tgagaccgta cgctgttctt tttgcgggaa
     4921 aacccaagaa gaagtgaaaa agatcgtagc tggtccaggg gtctacatct gtaatgaatg
     4981 tatcgaccta tgtaaagaaa tcattgatga agaattttat gaagaagctg tacgcgaatt
     5041 cactgaagtc cctaaaccac tagaaattct tgaagtattg aataactacg tgattggaca
     5101 agatcgcgca aaacgctcgt tagctgttgc cgtatacaat cactataaac gtgtcaatca
     5161 gcaagcacaa gaaaacacag atgatgttga actgcaaaaa agcaatatct gcttgatcgg
     5221 acctacaggg tctggtaaaa catttttagc acaaacatta gcaaaaacat tgaatgtacc
     5281 ttttgcaatc gcagatgcga caagtttgac agaagctggt tatgtggggg aagatgtaga
     5341 aaacattttg cttaaattgc ttcaatctgc tgattacaat gtggaacttg cagaaaaagg
     5401 gatcatctat attgatgaga tcgataaaat tgcgcgtaaa agcgaaaatg tatctattac
     5461 tcgtgacgtt tccggcgaag gtgtccaaca agccttgctg aaaattctag aaggaactgt
     5521 tgcaagtgtg ccacctcagg gcggacgtaa acatccacat caagaattta ttcagattga
     5581 tacgacgaac atattgttca tcgtaggtgg tgcatttgat ggcatcgaaa cgattgtgaa
     5641 gaatcgtatg ggcgaaaaga cgattggatt tggtacacaa aaccaaaaac tttctgaaga
     5701 cgaaagtgtc atgcagcata tcattcctga agatttacta aaattcggat tgatccctga
     5761 gtttatcgga cgattacctg ttatggcagc tttagagaaa ttgacaacag atgatctggt
     5821 acggatcttg actgagccga aaaatgcgct agtcaaacaa tatcaaaaac ttctttctct
     5881 agataatacc gaacttgaat tcgaacctga agcgttgaga gccattgcga aaaaagcaat
     5941 cgaacgcaat accggtgctc gaggacttcg ttccatcatt gaagaaatca tgatggatgt
     6001 gatgtttgat atcccttcaa atgaaaacat cgaaaaagtg attatcacaa aagaagcagc
     6061 tgaattgtct ggtgagccaa cagtaatcta taaagattca gagaaaaaag cgggttgatg
     6121 aaaagaggct gccgtaaaag tcattttggc ttttgcgaca gccgcttgct ttattcatca
     6181 ccttgttaag gagagagacc atgaaagtcc atcaagctga aattatcata agtgcagtgg
     6241 caccaaaaca atatccagat acacaattgc cagaaatagc tctggcagga cgatcaaatg
     6301 tagggaaatc ttcttttatc aacacattga ttgatcgaaa gaatttagcc cgtacttccg
     6361 gtaagccagg aaagacccag acattgaatt tttatttgat cgagaacgca ttacattttg
     6421 tcgatgttcc gggatatggg tatgcaaaag tatccaaaac tgagcgagca aaatggggaa
     6481 agatgatcga gacatattta acgactagag accagctacg tgcggtcgtt tctttagtag
     6541 atttgcgtca taagccttct caagaagata tccagatgta tcaatttttg aagtattatg
     6601 atatccctgt aattgttgtt gccactaagg cagataagat ccctcgtggt aaatggaaca
     6661 agcatgagag cgaaatcaaa aaagcgttgg attttgatcg caatgacacg tttattctct
     6721 tttcttccgt tacaaaacag ggaaaagaag aagcgtgggc agcaatcgaa caagcaattg
     6781 aatcataaaa agaataaaaa agacttacca gtcaaactct ttcataaaag tttggctggt
     6841 aagtcttttt ttgagcttaa cttacttttg tttcttgtct tttttctttt cattttgtgg
     6901 ctgtg
//
LOCUS       DACEMO010000142         6785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEMO010000142 DACEMO010000000
VERSION     DACEMO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.147"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            18..908
                     /locus_tag="IW728_002130"
     CDS             18..908
                     /locus_tag="IW728_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1025473.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(981..1805)
                     /gene="nadE"
                     /locus_tag="IW728_002131"
     CDS             complement(981..1805)
                     /gene="nadE"
                     /locus_tag="IW728_002131"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1025474.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1817..3289)
                     /locus_tag="IW728_002132"
     CDS             complement(1817..3289)
                     /locus_tag="IW728_002132"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1025475.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3926..4288)
                     /locus_tag="IW728_002133"
     CDS             complement(3926..4288)
                     /locus_tag="IW728_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1025476.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4511..5263
                     /locus_tag="IW728_002134"
     CDS             4511..5263
                     /locus_tag="IW728_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1025477.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5403..6326)
                     /locus_tag="IW728_002135"
     CDS             complement(5403..6326)
                     /locus_tag="IW728_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1025478.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6341..6613)
                     /locus_tag="IW728_002136"
     CDS             complement(6341..6613)
                     /locus_tag="IW728_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025479.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2071 a         1350 c         1161 g         2203 t
ORIGIN      
        1 tttaagaggt gatatatatg caactacaaa taaacgaaga atccttaccg gtatatgaag
       61 cattagctag taaaacaagg atcaagatca tccagctact ttccaaaaaa aagatgaatg
      121 tcaaagattt ggcaaaagaa cttggagtga gcagtgctat cacgacgatg cacgtgaaaa
      181 agctagagga agcaaacatc atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc
      241 ttcgtgtcga taaaatcgat atcagttttc cagaaaaaat attcaatgct tttgatacga
      301 aagaaacatc gatacctatt ggtcactata caaattatgc aattgaacca acatgcggtt
      361 tagctacgat ccatgatttt atcgggaaag tggatgaacc acgctatttt atggatcctc
      421 gaaggatgga tgcccgcatt ctctggttta ccagcggatt tgtcgagtat caggcaccta
      481 attttttaaa tacagaggat acattggaga tgttggaagt ttctgtggaa atttcttctg
      541 aatttccttt ttcaaatgac aattggcctt cagatatcac tttcagccta aatggtgtgg
      601 agctaggaac atggacgagc cctggagatt ttgctgatat ccgcggtaaa tatacgcctg
      661 attggtatcc agataatctt aatcaatacg gcttgctgaa gacgatccga attaccaaac
      721 acttgacgaa tatgaatgga gaacccttgt caaacatcac gatcaacgac atccctaaag
      781 agcaagacac ttggcatttg cgaatcgaag tgaaagacga tgccaaacat gtggggggct
      841 gtactttatt tggtaaagga ttcggtaatt acgatcagga tatcaaatta aaagtttact
      901 acagctgata tttttctttt ctattttata aaacaaaaac agccaatccc tatgaaggga
      961 cggctgtttt tgctttattg ttatttccag aaatcatcca aaacggaaat tggcaaatgt
     1021 cgtttatgtt gtgtcttgtt ccaccatgct tcgatttttt cttgtgcttg agcgggaact
     1081 tcttttcctt ctagataatc atcaatttca ttatatgtga cacctagtgc gacttcatct
     1141 gcaatcattg gtttcccatc ttcaagatct gcagtcggaa tttttgtgta taatttttcc
     1201 ggtgcattca atgcttgaag caattgtttc ccttgtcgtt tgtccaaacg gaataatggc
     1261 aaaatgtcgg ctccaccatc accaaatttc gtgaagaatc cagtaatgtt ttctgcagca
     1321 tgatctgtcc ctaatacggc tcctgctctt tctccagcta cggcatattg cgtaatcata
     1381 cgttgacgtg ctttgatatt tcctttgtta aaatcgctga ttcccacacc tgcttcgctt
     1441 aatacttgta cttgtgcatc aacagctggt ttgatattta ctcgcagact aacatcgggc
     1501 tggataaagt caagagcagc ttttgcatct tcttcatctg cttgttcacc ataaggaaga
     1561 cgaacagcga taaattgata gttttcatct tttgtttctt cacgcatttc ttccatcgtt
     1621 agttgcgcca aacggcctgc taaagtagaa tcttggccgc cgctgatacc tagtacaaaa
     1681 gttttaagaa acggatattt tttcaaatat gctttcataa agtcaatact tttacgtatt
     1741 tcttcttttg gatcaattgt tggtttgaca cctaattcat tgataatttc tgtctgcagg
     1801 ctcatttatt ctcctcctat gcttctgtat gaagttctgc tacgcttttt ttcattcgtt
     1861 ccatgatagc cattttatgg ttccagcatt cagtagacag atcgactggg tatttttgcg
     1921 gattcaagtc tcgtttatac tcttcccata gtgaatctag attttctttt gtatattctt
     1981 tgatttcgtt tagtgcaggt aattcgtaaa ctctttttcc ttcgataaag atatcttgta
     2041 aaactgggcg agctgtaaag tctcgaacag ttttattgat aaacgtatgc actggatgga
     2101 acatgaagat agcttcttct tctcgagggt cttcatccca taatgtcacg tagtctcctt
     2161 cagatttccc atcaaaattt cgagtgatgc gccacacttg ttttttccct ggagtagtta
     2221 ctttttctgc attgcttgat aacttgatcg tatccatcat ttttccttca tcatcttcga
     2281 tcgatactag cttgaatact gcaccaaggg ctggctgatc ataggctgtg atcaattttg
     2341 tcccaacacc ccatacatca attttagctt tttgcatctt taaattcaaa atcgttgctt
     2401 catccaagtc attcgaagca tagatttttg cttccgtaaa tccggctgca tctaattgtt
     2461 cccgaacacg cttagatata taggccatat ctccactgtc gatgcgtacg ccttggaagt
     2521 tgattttgtc acctaattct ttcgccacgc gaatcgcact tggcacacct gatttcaacg
     2581 tatcataagt gtcgactaag aacacgcagt ctttatgggt tttagcgtag gccataaatg
     2641 cttcataatc atttccataa gattggacta acgaatgagc atgggtcccg ctagctggta
     2701 tcccaaatat cttcccagca cgtacattgc ttgttgcatc tgctccgccg atatatgccg
     2761 ctctagttcc ccagactgct gcatccagtt cttgtgcccg tctagttcca aattccaata
     2821 gtggatcttc tccgatgaca gatttgatac gagctgcttt tgtcgcgatc aacgtttgga
     2881 aattgaccat attcaacagt gctgtttcta ctaattggca gtgcgctaaa ggcccttcca
     2941 cttggattaa tggttcattt gcaaaaacta gttctccttc acgagcagaa cgaactgttg
     3001 ctttaaattc aaaattttgt aaatactcca aaaaaccttc aggatagact tctaatccac
     3061 gcaagtaagc aatatcggaa tcattgaacg tcagattctc taaataattg actaaccgtt
     3121 ccaatcctgc aaaaaccgcg tatccgtggt taaaaggcat ttctcgaaag taacattcaa
     3181 aaacagcatg taaatcagct cgacctaatt cccagtatgt ctgcatcata ttgatttgat
     3241 atagatccgt atgcagcgtc aaactgtcgt ctgggtaagt attattcatc ttgtattact
     3301 cctcttggca agttttcatt tcctacccat tataacaaaa aaaattaata ggaaaagaaa
     3361 tcttccattt tttctgaaaa agcacatgtt ttacggatat ttcttgctaa aaatgaactc
     3421 catctgctaa aactatcctt tcttattggt atttaaccta aacgagaaag aatacttatt
     3481 ttattcaatg tatccgagac acgaagaaat tagcgttttc tatttttctt acttgataaa
     3541 ataagtagat tttcctacta caatcgtagc cgttaagata ccgatgattg aacaaatata
     3601 accaaatgtc tctacaacat aatcaatggc aaaagttttt gaagcaataa tacgtctttc
     3661 cttttttgct tcttatcccc aaagaaacgg atataactta ttatgtattg aatttttttg
     3721 gaaaggcgaa ttatgacaag atataccttg cgttgaaaaa actcgcggaa aaaatatcac
     3781 acgtttttaa aaagatacaa agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg
     3841 cgagacatca atcagtctcg ccttgttttt tattgtatac attagatatg gagcaggtta
     3901 ccataaagat gattattagt cgttattaaa tagaatcctt cgtatttttt tgttctatca
     3961 ctacaatatc tttatttgga cgtttgaatt gtattccggc acgttctagc tcctgtttca
     4021 ctcctacatg gactttgaca tcttctcgtg tcatttttaa aacgtgatga atcgcatagt
     4081 cgatttcttg ccttgtgatt tttttcttcg tattgatcat cacgacttgc tttttatcat
     4141 atgtgtctac caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa taattaatag
     4201 ccgtattccc aaaaaagaat tccactactt taggataaaa tgtcttaata tttaaattac
     4261 tattcacagg tgtttcgatt agcttcatct cattcctctt ttctatatag tcgatttaga
     4321 tccaatctta agttatgtta ccatctttcc tagcattaag gaatcggtta caattgaacc
     4381 tttaaagaca aaaggattta ccttttatca cttaatagta taacataatt tcgtgagaaa
     4441 aaaatacaat tttttcttga ccatctcgtt acgtaaagga ttatgcttac atttgtcagg
     4501 aggaaaaata atggagtaca caataaagaa aatgtcagag atatcaggag tcagtccgag
     4561 aaccttgcgc ttttatgatg agattggact gctaaaacca gctaggatca attcttcagg
     4621 ttatcggatt tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat tttatcgaac
     4681 aatggcattc aagttggatg atatccaaga ggtcttagac aatccatctt ttgaccacca
     4741 gaaagcttta atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct
     4801 cttgacaact gtccaacaga cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa
     4861 agaaaaattt gaagggttta aacaacaaaa gttgaaagaa aatgaagaat cctatggaca
     4921 agagattcga caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa
     4981 tatggataaa gaaacgtatg aagaaatgca aactgttgaa aagcagatgt tatttgatct
     5041 cactctttac ttaaaagaac cagaagatga aagcttagca gaccgtatct tccaagctca
     5101 taagaagtgg ttctcttaca gctggcctga atatcagcca aaagcgcata aaggactagg
     5161 aatgatgtac gaagccgatg aacggttcac tgcatattat gacgagcgaa gtggtcatgg
     5221 tgcagcaaaa gctttaaatg aaattatcca aaaaaaggcg taatctttta catgatccaa
     5281 caaaaaagta cacgtaaaat gacctaataa caaaacagca gacacccaag ttagattttt
     5341 atgctaactt ttggtgtctg ctccattctt tcacaagttt tttcatccta ctttaaatat
     5401 attcatcgtt gaataaacgt ataaggtatt ttcactttat cctttttttc tgtaagaaac
     5461 aattgaaatg ccatttctcc gcaagcttcc agatggtggt cgatggttgg gaaaccaaga
     5521 agctcgctga tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc ttgcagatag
     5581 ctttgcaaga tgcctgctgc aaccatatcc gaatttgtaa agacaccatc aactccaagc
     5641 tctttaaaaa agaagcctgc ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca
     5701 agtgattcat caaaagatgg aaaaagttga tgacatagct gcaaagtgat ttttgagtta
     5761 taacttagcc tgccacttcg ccccaacgtg atgcccaatt gctttatacc ttgctgcttc
     5821 aaataagtaa gaccttctgt aattgattgt tctcgatcaa taaagacaca gcttgcctgt
     5881 tttcccttga tttcttcaca aaaaactaga ggtccatatt tttgatatgc caataaatca
     5941 tctagtgtat tggcacgaga cgtaatgatc aatccatcga aagctttagc tgcaaattct
     6001 tcaagatacc ttcgctcttt ttctttatga tagcccgtcg gtaaaagtgt cactttgtaa
     6061 tcataatcaa acgctgcttc aatgatccca ctgatcaact gatcaaaata cggatgattg
     6121 gtataaggca gaatcactcc aatgcttttc gtttttccat aacttaactg tctggcattt
     6181 gcattaggga tataatcgac ttctttcatg accgcaagta tttctttcct tttttcctca
     6241 tctacatatg gatgtccatt gattacacgt gaaatagtag aaactgaata acctgtgagc
     6301 tttgcgatat ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa agattttctt
     6361 gtaaatagtc taccacagct ggaccgcatt caaaaaactg atacattttt ccttcatgcg
     6421 aaaaactgat ccgctgcccg ccccaatata tatcttttgt caaattttcg atttcattca
     6481 ttggtatttc ttttactata ttcctattca acccttgaaa actgtacaac gttaatacgg
     6541 atcccttgaa ctcgacaaac gcataaaaat aattgttcgt tgtgccatct gttatctcta
     6601 cataagtatt cattgcaagc cctccttttg atactttgag tatagtgtat gaaacgcgtc
     6661 tcatagcaag gagtttgttt tgccaaagaa aagttttatt cctctttcat tattaccaaa
     6721 gtctcttatt ttaaattgaa tacattataa tgatactgta aatatatctt tattattagt
     6781 gaatg
//
LOCUS       DACEMO010000143         6649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEMO010000143 DACEMO010000000
VERSION     DACEMO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.148"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..514
                     /locus_tag="IW728_002137"
     CDS             <1..514
                     /locus_tag="IW728_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025480.1"
                     /translation="MTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGMD
                     YFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVY
                     AYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLC
                     LFFKIVFHKR"
     gene            689..1522
                     /gene="msrA"
                     /locus_tag="IW728_002138"
     CDS             689..1522
                     /gene="msrA"
                     /locus_tag="IW728_002138"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1025481.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1602..2591
                     /locus_tag="IW728_002139"
     CDS             1602..2591
                     /locus_tag="IW728_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ1025482.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2880..3578
                     /locus_tag="IW728_002140"
     CDS             2880..3578
                     /locus_tag="IW728_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ1025483.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            3728..5044
                     /locus_tag="IW728_002141"
     CDS             3728..5044
                     /locus_tag="IW728_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1025484.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            5061..6476
                     /locus_tag="IW728_002142"
     CDS             5061..6476
                     /locus_tag="IW728_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1025485.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
BASE COUNT         2258 a         1048 c         1392 g         1951 t
ORIGIN      
        1 tatgactgtt ttgttattaa tcactttaac tgtttctagt aaaaaaaatt tcaatcaaaa
       61 aataatttct cagccgggac atattgttgt aaacaaagaa acagtagagg cgtataccac
      121 taaaggaatg gattattttt tatatgatta ttctcctatc gcttcattgg agaataagga
      181 gcatataata aaacacgaag caaagattgg cagtcaatgg gtaaaccttc cttataaaac
      241 aactggtagt tctatcatat atagagtaac taatgagaag agccaagtaa tcacacttcc
      301 tgtatatgct tatttaggaa cgaatgtaga aataaatcat caacctgtaa aggagcaggt
      361 gaatgctaat ggattggtac aaataagcgg agtcaaagga gttgctgata ttaaagtaag
      421 cacaagttat acagtaacga ctattatttc ttatcttgtt acattaactg catatttatg
      481 tttatttttt aaaatagttt tccataaaag gtaaattaac ttttcattta taaaataaat
      541 tgattaaaaa ggagaatctg tcgataagga ttctctcttt tttatacgaa aaatctgtag
      601 ggggatgaag taatcgctta tttcctcact ttatgataaa ttacagctaa agtcactagc
      661 ttgtcttctt cggaaaggag gagaatctat ggatgaggaa caaatattca ttcaaacact
      721 ttataatctg attttgaatc cgaatacacg agattgggaa cgaaaagtat tgattcagac
      781 aaagaatgac ataagagaaa atatatccgt taaagaacag ttatcaaaac tagaagcaac
      841 actacgtcca ttagctatcc gaatgaatct gacgcctgat gtaatggact tttatttatt
      901 gattacggaa ggatttgata aggagcaaaa atatgacttt tccaagcatg ccatgcaaga
      961 tgctgactat caagaacgtg cagtatttgc agggggatgt ttctggtgta tggtcgagcc
     1021 gtttgaatcg aaaaaaggga tcctatctgt tttgtcagga tacacgggcg gacatgttga
     1081 aaaaccaaat tatgatcaag taagtggtgg ttacacaggt catgtggaag ctgtggagat
     1141 catttatgac acaagagaaa taagttatag cgagttgctg acaatctatt ggcaaattac
     1201 tgatcccaca gatacatttg gacaatttca agatagaggg aagcaatatc gtccagtgat
     1261 tttttatcaa gtcgaacgac aaaaagaatt agcggaacaa tcaaaacaga aactagacag
     1321 ttcaggaact ttccaccaac caatcgtcac aaaaatcgaa ccagccggta cgttttggcc
     1381 agcagaaaac tatcatcagc aattttacaa aaaacaaccg aaacgttata aaaaaatcca
     1441 gcaagcacgt aatcaatttc tgatctatca acgagtaaaa aacaaatggc aaaaaaatat
     1501 acgaaaaaat cattttgatt aactcctttt ctttaaaatg tagtaaacta aaaatagaaa
     1561 aaagaaagcg ttttaaaaag atttgatgag gaggagaaaa attgtatatt gcagatagtg
     1621 cacgttatga aaaaatggaa tatcgtaaat ccggtcattc aggtttgaaa ttaccgattt
     1681 tatctctcgg attatggcaa aattttggtg attatgatcc tatccataat caaagagaga
     1741 ttttaagagg cgcattcgac atgggaatta cccatttcga ccttgcgaat aattatggag
     1801 gccctgctgg agctgcagag aaaaactttg ggcgtatttt tagagaagat tttcaagcct
     1861 accgtgatga actgattatt tctagcaaag caggttatca tatgtgggaa gggccatatg
     1921 gggaatgggg ctctcgtaaa tccatcattt caagttgtga ccaaagtctc caaagaatgg
     1981 ggctagatta tgttgacatc ttctatcatc atcgcccaga ccctgataca cctttggaag
     2041 aaacagcaga agctttgatg caacttgtaa gacaaggaaa agcattatat attggtatat
     2101 ccaactataa tggagaagat acaaaaaaaa tgactgaaat tttaaaaaga aaggaagcac
     2161 cgttcattat tcatcagatg cgctacaata tgttttctcg tgcactacta gaagacgatc
     2221 tatctccagt attagaagaa gaagggctag gggcaatcac gttcagtccg cttgctcaag
     2281 ggttgctgac aaatcgttat ctacatggta ttcctgaaga ttctagagca catcgtaagg
     2341 aaataccatt cctttcagaa gaacaagttg gatcaactct ggaaaaaatc aaagcactgc
     2401 aaacaatcgc cgtatcacga ggacaatcat tagcgcaaat ggcccttgca tggaatttgc
     2461 gacaaaaatc tgttacaagt gtactggtcg gagcaagtcg gttaagtcag ttacaagaaa
     2521 gtgttcgaat gatggataat ttagatttct caccagaaga agaattgaga atcgaccaaa
     2581 ttctagaata gaaacgatgt aatcaaactt tttatccata tattaaaaaa aacaaaagat
     2641 gacatcaaga atcgaatgaa gatcgtctgt tattgaacat catatttttt gtcgttcaaa
     2701 ataaaaatga tcgaaggcta ggagtgaatt ttatcctagt cttttttatg gtaaatatct
     2761 tcttcgtttg tatgtctatg aacggattaa tcaattttgt atacaaaaaa tcatcagatg
     2821 attcaaacgt gttataataa ttacgaaaaa atacatcaga tgaaaaggag gtatgtggtt
     2881 tggaagaaaa taaaacccta gtagaagtaa ctactgacaa gatccttaga tatttaaagg
     2941 aaatgcagtt gcaagtagga gataaactgc cgaatgaata tgaattgtcc gattatttag
     3001 aggtggggcg aagcacggtg cgagaagctg tgcgggctct agcttccagg aatatccttg
     3061 aagttcggca aggagcgggg acatttgtca gtgataagcg agggattgcc aatgacccac
     3121 tcggtttttc tttgattgaa gatcaggatc ggatgatacg agacttattt gaactgagat
     3181 atttattgga gcctaagatg gcaggtttgg cagcggaata tgcaacgaag aatcaaatcg
     3241 tccaaatgaa aaaattaacg caaaagattg aaacttcttt tcgagaaggc acacaagaac
     3301 atgttcaatt agacatcgat tttcatacga tgattgcaga agctagcgga aacgtagcct
     3361 tgctgcatat tatcccaatc attaatgaat caattgcttt gtttaataaa aattatgata
     3421 tccagcatct gaaaatcgag acgatcgata ttcatacaga gatcgttcag gccatcgaaa
     3481 ggcggaatgc acttgctgcg tttgatgcaa tgactgtcca tatggcaata aatcggagag
     3541 aattacagaa aataatagga gaaaaagaaa ctaattgaca gttcatctga tgtattttat
     3601 gataaataca tcagatgaat ttttgtttta tatttggtaa aaaagaggag taaatagttt
     3661 ctgttcatcg aaatgtaagc gctgactaat agacaaaaag agatgacgtc aaggagaaga
     3721 agcaggaatg aggggatctg gaatgagttt tatggataaa ttagccaatg ttttagggaa
     3781 cgtggcaacg agaatcaata atttaaggta tatcatggtg ataaaaaacg catttgctgc
     3841 attgattccg gtaattatta cgggtgcttt tggcacattg ttttcagcaa tggtctttga
     3901 ctcggaaaat ggcttagcac agataagttt cttgcgtttt ttagaagaat taaagcctat
     3961 cgcatctgcc atcagctatg tcacactaag tttcttgacc atctatgctg tttttctgat
     4021 agggatcgaa ttagccaagc tgaataaagt ggatggtgta ttccctggta tcatcgcagt
     4081 gatgtcttat ttatctgtta atccaactgt ttatgaatta gtgaatgaaa atcagactgt
     4141 tatagtagaa aacgtattgg ctaaacaata tacagatacg aaagggttat ttttgggaat
     4201 gttggtctcc atcctttcta ttgaacttta ttgctggttg aggaaacaga agaaactaca
     4261 gctgagaatg cctgataccg tgccaacgaa tgtttctgct agtttttctg ctttattccc
     4321 gacgattctg actgtatcgg gtatcgcggc tgcgggattt atcatcaaag aacttacagg
     4381 aatgtacctt tatgatatca tttacaatgt cgtacaacgc ccattagaag gtgtcgtaca
     4441 agggttaccg ggtattttgc tattgatgtt tattgcgcaa gtcttttggg tgattggtat
     4501 ccatggaaat caaatggtga agccgatacg ggaaccgttg cttttagcgg caattgctgt
     4561 aaatacggaa gcttttgaag ctggaaaaga agtaccgaac attattacta tgccattttg
     4621 ggatatgtat atgagtatgg gcgggtctgg agtcacgatc ggtttactga ttgcagtatt
     4681 gttagttgga aaacgggaag atatgaaaca aatcaccaag ttatcccttg ctcctgggat
     4741 cttcaacatc aatgaaccag ttatttttgg tatgccgatc atgctgaatc caattttagc
     4801 tattccgttt atcttaacgc ctcttatcac tggaacgatt ggttattttg caaccgcttt
     4861 aggatttgcg gcgaaagcag ttgtaatggt cccttggcca atgccgccga tcgtcaatgc
     4921 ttatctggca acagcaggaa atatcggtgc agttattacg caacttgttt gtattattgt
     4981 ttctattttg atctatctgc catttgtaaa agtagcaaat cgtgtggaaa atgatgggat
     5041 ctaaaaaaca ggaggaaatc atgaaaatta ctattccaaa aaattttatt cttggtgctg
     5101 catcttctgc ttggcaaacg gaaggttgga aaggaaaaaa agaaggacag gattcgttta
     5161 tagatagctg gtataaaaat gaacattttg tttggcataa tgggtatggc ccagccgtag
     5221 caactaattt tatggaacaa taccagcagg atgtggatct gatgaaatcc atccatctga
     5281 cacattatcg gacttctatc aattgggctc gtttttttac tgattatgag aactgtatag
     5341 tagatgaaga atatgctcag catataagtg aaatgatcga ctctttgatc gaagcagacg
     5401 tagaaccgat gatttgtttg gaacattatg aagtcccagc ttttcttttg gaaaaatatg
     5461 atggctggtc ttcaagaaaa gtagttgatt tttatgctaa gtatgctcaa attgcctttg
     5521 atcgttacgg tgatagggta aaaaactggt ttacattcaa cgaaccaatc gttccccaaa
     5581 cgcgttgcta tttggatgct gttcgctggc cgcatgaaca aaacacaaaa aagtggatga
     5641 tgtggaatta tcataaagtt ttagcaacgg ctaaagcagt agagatttac cgcagcggaa
     5701 attatggtgg aaggattggc tgtattttaa atcctgaaat ggtctatgct cgatccaatt
     5761 ctccagaaga ccaaaaagct gcttatatgt atgatttgtt ttataatcga gtattttttg
     5821 atccgatggt aaaaggatac tacacagaag agttcctgga gttgtgtcaa aaacaatcga
     5881 tttatttcga tcctgcaaca gaggatttag aaacgattca gcatcataca gtagatttcc
     5941 ttggaatcaa tcaatattat cctaaacgcg ttcgcgcccc tcgttatcaa tggaatgaag
     6001 agacaccttt ccatcctgaa cgatattatg aagtctttga tttacctggg aagaaaatga
     6061 ataattccag gggatgggaa atctatccga aaatcatgta tgatatagcc atgtatctaa
     6121 aagaaaatta tcataatatc ccttggctga taacagaaaa cggtatggga agagaatatg
     6181 aagaacagta tatggatgag aaaggaatcg tgcaagatga ttaccgtatc gaatttatcg
     6241 gacagcattt gcattacttg atcaaagcaa cagaagaagg cgcaaattgc gaaggctata
     6301 tgttatgggc attcactgac tgtgtgtcac caatgaatgc atttaagaat cgatatggat
     6361 tagttcgaat cgaattgaac gatgaacgaa gcagaacatt gaaaaaatct gcttcgtggt
     6421 atcagacttt gattgataag agagaattgg atttgcctga cgaaatctat aaataaatag
     6481 aagttttttg tagatgttca ttgtatttac tggttatttg aaataaaaga ggttgtgaat
     6541 ccagcgtttt gagctaatac ccagggtcag ctgattgaac accgtttatt gagaggttgt
     6601 gaaaaagctg tcctgcatca agtaataaga atagcaaagt aaaaacaac
//
LOCUS       DACEMO010000144         6569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEMO010000144 DACEMO010000000
VERSION     DACEMO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.149"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..297
                     /locus_tag="IW728_002143"
     CDS             <1..297
                     /locus_tag="IW728_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025486.1"
                     /translation="ERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLL
                     SLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(423..1100)
                     /gene="nth"
                     /locus_tag="IW728_002144"
     CDS             complement(423..1100)
                     /gene="nth"
                     /locus_tag="IW728_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1025487.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1107..1805)
                     /locus_tag="IW728_002145"
     CDS             complement(1107..1805)
                     /locus_tag="IW728_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1025488.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2073..3257
                     /locus_tag="IW728_002146"
     CDS             2073..3257
                     /locus_tag="IW728_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1025489.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3263..3706)
                     /locus_tag="IW728_002147"
     CDS             complement(3263..3706)
                     /locus_tag="IW728_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1025490.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3932..4219)
                     /locus_tag="IW728_002148"
     CDS             complement(3932..4219)
                     /locus_tag="IW728_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025491.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4390..5742)
                     /locus_tag="IW728_002149"
     CDS             complement(4390..5742)
                     /locus_tag="IW728_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1025492.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6015..6461)
                     /locus_tag="IW728_002150"
     CDS             complement(6015..6461)
                     /locus_tag="IW728_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1025493.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1780 a         1299 c         1083 g         2407 t
ORIGIN      
        1 gaaagaagct ctcatgcaat gattctcact tttttatggt tgttgttcac cagaatctat
       61 atttttctga tgagcctgaa accgcccaaa tccattagtt tatgtttagt tataaatggt
      121 atttttttat tatcacttat catttttatt acacctttag atagatacgt cacttattta
      181 tataatcctt tggaatactg gacttatttt ataggtatat tggaaggcat tattttctac
      241 atcgggtatt ttcccaataa aaataacaac ttttattttt atcgcaataa attgtaataa
      301 gttaccatcc tagctactga gtatgtacat ttactttttc atgcaaaaaa gataaacgaa
      361 acagcttttc tccataataa caagataatt catacattta tttgcaatta aagaaaccac
      421 gtctacaggc gtggtttctt ttttagcgcc ttttctttta gacgcatccg atttttccca
      481 tcttgacata ttgatagcaa aggacatacc tcgcacttgg gatttcttgc cgtgcaatga
      541 tatctaccaa aaaaaatcag tgtatgatgt gtctttaccc ataattcttg tggtactttc
      601 cgcatcagtg tttcttctac ttccatgacc gtagcatcta gtttgcagat cctcaggcgt
      661 tttgagacac gttccacgtg ggtatctact gcgattgctg gtatcccgaa tgcatccccc
      721 aatacgacat ttgctgtctt tctgcctaca ccaggaagag acatcaattc ttctcttgac
      781 gttggcactt gcccatcgaa acgttcaatc agctgctgtg cacacgcttt gatgtttttt
      841 gctttatttc gatacaaacc aatcgttttt attttaagaa tgatttcatc gattgaagct
      901 tctgctaacg catcgggtgt cggaaaagag gcaaacaaat ctggcgttgc tttattcact
      961 gatacatctg tcgcctgggc acttaagatc actgcaatca ataactcaaa tggattattg
     1021 tgcttgagtt ctccatgtgc ttcgggaaac attccataca tcgtttctag agcttccatt
     1081 gttttttgtt tgcttaacat attccgctac tcctccaacc aatttttcag tgagactttc
     1141 ggcaattctt cttgttgagc tgactgttct acttctcttt gaagaagctg ctgttttctt
     1201 cgtttttggt cttcttccac ttgttgtttt gtacgtagat tttttctttc ccacgatagc
     1261 aaaatgcggt caacgtaatt aaaactataa gcctgattta atacggcttc ccgcagtgct
     1321 agcttcaaaa tttccggctg ataatggtct tcttctagcc attgtccgat ccgctggaac
     1381 tcgatcgaag aaagcgggcg tccaaattct tgttcaaata attgatacac tgactggatt
     1441 tctttttctt gtgttttttc ttcgtatttt ttttcttgtg tttttaaata ctcacctaat
     1501 agatcataga tggggtaaag attatatcga tctgcttttt taccgttgac aatcgtcgtt
     1561 tctatcttga taaatccatt tgttacgaga cgatccagta tttgaaaaat ctgatcttgt
     1621 ttgattccca tatccatagc aatcatctgc aaatcaggga aagtatctcc ttcaagctgt
     1681 gccatatgca actgtagaac aaataaaaat tcttcagatt gtagaccgat cctatgataa
     1741 tttttaagta aaagattaga tatgactgtt tgtccagcgc ttaagtaatt ttctaaattc
     1801 aacatgattt ctcctccgct caatcacagt atacgtaaaa aaaacatggg aagcaagaac
     1861 aggtttcttt tctatttact tcactgatcg gtcagattta caatgtcgtc tacttgattt
     1921 ttgtttgttc atgtttttga tgcgtatctt cacttttcgg aataaataaa ttaagtagat
     1981 ttcgctgttc cttagaatgt tgacaaggaa acaaaattac tatacactat ttttgtttcc
     2041 aagttaacat tattgcataa aggagttttt acatgtacag tttcacaaat aaacattcta
     2101 aagtttttgg atacgtctct gtcttattca ctggattagg atttactatc gtcagtcctg
     2161 tacttccgtt tttaacgctg ccttattctc actccataca tcagcaagct ttctatatca
     2221 ctttgctcat gtctgtttat gctctagctg ccttcttatc tgcccccatt ctcggtagct
     2281 taagtgatca ttttgggcgt catccgatcc ttattatcag tttattagga tcaagtatcg
     2341 gatatttgct ttttggttta ggaaattcga tatggatgtt gttccttggt cgtatcattg
     2401 aaggattgac ggcaggagaa atctccacct tatatgctta ctttgccgac atcacggaac
     2461 ctaatgaaag aaccaaagtt tttggatgga tgggtgcttt agtcggtata ggaacaacgc
     2521 tgggtcctat tatagggggt ctattagctg agttggggaa ttctgttcct atatttattg
     2581 gagcactctt tactttctta aatgccgttt atggctacac atttatgcta gaaagccttc
     2641 caataaaaaa acgttcagta gatttaagtt tctcccatgt aagacctttt caccaattac
     2701 aacaattatt caagatttca tccgttattc ctttattgac agcaggtttt gctgtttggt
     2761 tggcagctgg ctctcttcaa tctatctttt ctcaattttc gattgacgcc tttcagtgga
     2821 aggctgggtt ggttggttta agcttctctt tgattggaat cttggatact gtctcgcaat
     2881 tgttcattat gcctcgattg ctcaaaaaat tttcagaaca gcagatcaca agaataggga
     2941 tgttcagtga aatcctcgcc tatctattca tcacattatc tggaattctt ctactgccga
     3001 ttttgttttt atttgggatt atctgttatg gtttcggtga ttctattttt actcctgtat
     3061 tcaatggcca actatccaat tctgtctcag ataaccagca aggtcttgta atgggcggta
     3121 cccaaagtat acaatctcta tcgcgcgtca tcggcccgct aatcgcagga cagctttatg
     3181 ctgcagcacc atttcttccg acggtattcg gatttttgct tatgattttt gcctattatc
     3241 actacaataa aaaatagtag cttcatttgc tacttcgtat aaattctctg tatcgaccga
     3301 taaaacgaaa tactgtgtca aattcttctt ttgacatatt ttcaaataca gtagcatctc
     3361 gctgtaccca tttttgatgg agttgctcgt gtctattcct tattttttgt ccgattggcg
     3421 tcaattgaaa atagatttct tttttattct cttcgctttg ataagtgttt atgatccctc
     3481 tagctatcag tttttttgtt agcttactga tggctcctct agtcatgtaa ctagcattcg
     3541 ctaattttgt cacgttagga tcagggattt tcccgatatg ttcaatcact tctagttcat
     3601 ttagagtaaa tcctgcaaat tctttttcca tttctggttt attacgatga ataatctcat
     3661 tagctaaatc cagatattct tctattagca gttgtctttt atccatttct acaccttctt
     3721 tttctttcat tatattgttt ttttgtttcc tattcaacaa agataaaaat attttgtagg
     3781 aaaacccaat ctgaaaccaa gaaaaaaagg ggttgacaaa gcttgtcata accccttttt
     3841 ctgattaaaa gatagtaaag agacagtcat cggtaattgt gatgattttt tgtcatcact
     3901 taaggatgag cggccttttc aaaaactgtc tttaaagatc atcatcagtt tctagcacat
     3961 atggctgacc ttcatatgtg tactctgtat tcaatgtgcc gtcattttca tctgagtaga
     4021 tgcgtctact gaaaggaaaa cgatgagaaa cattatcttt ggcgtcattt gctggcatcg
     4081 tgtaatattc ctcttgcgtt tcattcaact gagagagtac ttgatctgcc aactcatcaa
     4141 tattgatcgt taatttcttc gaagcacttt tataatcttc taaaaatgca tcttgttgga
     4201 caaaatattc tttattcatc ttctttctca cctttctttt caaaatcaaa taaagctgct
     4261 tgttctttta tgtaacagct ttatctttgc tgcttattgt gacaaaaaat tcgaagaaag
     4321 acaactaatt gtacttttca caaaaaaaga cagagggtcg tgacaaaatt gtgtcacaac
     4381 cctttttctt tattgtgcag tttcgatttc gcctagcgct gcttgtgcaa gtaggttcaa
     4441 gtaattccat gggcggtcaa aatgtggttg gaagaagaaa tctgaaagtg cgagatcttc
     4501 tacagtcatt ttattttgaa cagctaaaga catggtattt gctgattgtg taatgtcgta
     4561 tttggacatg aactgagcac ctacgatacg attcgttcct ttttcataga ctagctccat
     4621 cattactttt tcagtagttg gcatgaactc tggacgataa ttatcttcaa atacagtcgc
     4681 ttggacttct agatcgttca aagttgcgct ttctttggtc acacctgtcg aaccgatcgt
     4741 ccagccaaat aggtaaagtc ctgaagttcc ttgagtacca cgatacgcca ttttatgatc
     4801 tgtcaagtta tttcccacaa gcaaaccttg acgaacggca tttgttgcca aaggaatata
     4861 gttttgcgtg ccacttggat tgtagttgac tactgcagag tctccagctg caaaaatgtc
     4921 tggattactt gtctgcatgt attcattcac tttgattgcc ccgtttggca gtgtatctac
     4981 ttttccttcg atcagttttg tatttggacg gaagcccaca cataaaatga ccatatctgt
     5041 atcaaatgta tctgttggtg tagctacttg tttcactttt ccttcttcat ctgtgataaa
     5101 ttcagtaaca ttttcgccta gcgcaagagt aacaccgcgg tctgtcaatt ctttttctaa
     5161 gatatctgtg aatggtttat ctagatattt attcaagatg cgatctaatc catcaatcaa
     5221 tgtcacttgt tttccagatt cgacaaaagc ttctactaat tcaataccga tatatccacc
     5281 accaacaatt actacacgtt ttgcttcttt cgcttgtgca ataatttcat ttgcttgatt
     5341 gtagtttttg cataggagta catttttcgc atcgattcca ctaatcggtg ggataatagg
     5401 ccatgaacca gttgtcatca ctaatttatc ataagaaacc gtttctgttt ctcctgtttt
     5461 cagatctttt gccgttactg ttttattttc tgtatcgacg tctgttacat cgtgttccat
     5521 tttaactgtt gctcctaatg aagataattc ttcaggattt gagtagaaca agcctgctgg
     5581 gtcctttact actccgccga cataaagtgc gataccgcaa gataagaaag aaatattgtc
     5641 atttctttca tatactgtca cttctgcttc tggatgattt tttaaaatac tttttacagc
     5701 tgcagtacct gcatgagtac agccaattac tactactttc atttatggtc cctccgatta
     5761 acattttgtc cttcgacaaa actactataa cagaaataaa acggatacaa cagtttaatg
     5821 cttgtgaatt cacaaaggtt tgtttgtagt ttcattcctt ttcaagcata agaacccaat
     5881 atctacaaga ttcttatttt aataatattc acttttatat ctaagattgt gaataaaaga
     5941 caaaaaaata aaaaagctag cgtttttttt acacagctag ctttttttag gtataacact
     6001 tgtagaaact gtttttacat ttgcgccttt tttgctgact ctttacaatc cggacagagt
     6061 ccatacaatt ccaaccgatg ttcattaatc tcaaaatcag tcagtctgct tgcagccatt
     6121 tctatatctt caagccctgg gtaatgaaaa tcaacaatct taccgcagtt ttcacaaatt
     6181 acatgataat gacgctttga actgaaatca aatcggctag aagcatcgcc gtacttcatt
     6241 tcttgaacaa atccaatcga agtaaataag cgaaggttat tgtaaacagt cgcaacactc
     6301 atatttggaa accgatgttc aagtgcttta taaatctcat cagccgttgg atgggaatga
     6361 tgttcaatca aatattctaa aaccgcgtac cgttgaggtg tgattcgaat gttctctttt
     6421 tttaactttt cgataacctc ttcgactaac ttattttcca tgtaaacacc ccacgttgtt
     6481 attctcgtct tatcatacta tttttacatt ttaaaatcca cttaacatga aaagaaaatt
     6541 caattttatt gtattttaat gaaaaaaga
//
LOCUS       DACEMO010000145         6455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEMO010000145 DACEMO010000000
VERSION     DACEMO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.150"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1014)
                     /gene="tsaD"
                     /locus_tag="IW728_002151"
     CDS             complement(<1..1014)
                     /gene="tsaD"
                     /locus_tag="IW728_002151"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1025494.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1011..1475)
                     /gene="rimI"
                     /locus_tag="IW728_002152"
     CDS             complement(1011..1475)
                     /gene="rimI"
                     /locus_tag="IW728_002152"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1025495.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(1482..2024)
                     /gene="rimI"
                     /locus_tag="IW728_002153"
     CDS             complement(1482..2024)
                     /gene="rimI"
                     /locus_tag="IW728_002153"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1025496.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2008..2832)
                     /gene="tsaB"
                     /locus_tag="IW728_002154"
     CDS             complement(2008..2832)
                     /gene="tsaB"
                     /locus_tag="IW728_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1025497.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(2921..3901)
                     /locus_tag="IW728_002155"
     CDS             complement(2921..3901)
                     /locus_tag="IW728_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1025498.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(3925..5409)
                     /gene="galT"
                     /locus_tag="IW728_002156"
     CDS             complement(3925..5409)
                     /gene="galT"
                     /locus_tag="IW728_002156"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1025499.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(5421..6410)
                     /gene="galE"
                     /locus_tag="IW728_002157"
     CDS             complement(5421..6410)
                     /gene="galE"
                     /locus_tag="IW728_002157"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1025500.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         1713 a         1441 c         1143 g         2158 t
ORIGIN      
        1 ttcagataaa accaagcttg gatcagcatt caactgatag ctagcaaaat ttcttttttg
       61 catttccaca tgtgctgctg ctccaatcat tgcagcatta tctccacaaa gtcgaagagg
      121 gggaatcact agttctacct cagggagttt agttgccaaa gcatcctgca atccttcacg
      181 caacccttga ttggccgcta cacctccagc tataatcagt tgcttgactg gataattttg
      241 acaagctcga agtgttttag taatcaatac atcgatcaca cttgcttgaa agctagctgc
      301 taaatcattt ttgtctagat cttctccacg ctgttgtgca ttatgcacca aattgataaa
      361 tgcacttttt aatccgctaa aactgaaatc ataattatct tcatggatca tcgctcgcgg
      421 aaaatggtaa ttatcttttc cctgatgagc tagctgatcg atttcttttc cgcttgggta
      481 agaaagacct aatactcttc caactttgtc ataagcttct cctgctgcgt catctcttgt
      541 ctccccaatg atttcatatg atccatcttc ttgcatatag actaattctg tatgtcctcc
      601 gctaacaaga agtgccatca atggaaactg gaatggttta accagacggg ctgcatagat
      661 atgccctgcc atatggttta caggaataag cggcaattgg tgtgcccaag caaaagcctt
      721 agctgcagaa ataccaatca gcaacgagcc gacaagtcct ggtccataag tgacagcgac
      781 tgccgataaa tcttctgccg acacaccagc ttctaccagt gcatcttcca agcataaggt
      841 gatttgttct acgtgatggc ggctagctac ttcaggtact acgccaccaa aacgtttatg
      901 actgtttatt tgagaagcaa cgatgttaga taagatctct gttccatttc taacgaccgc
      961 tacactcgtt tcatcacaac ttgattcaat cgccagaatc aattcttcat tcatttcatc
     1021 gtctccgttt ttaactttct gctcataatg atcgcatctt ccgtcggatt gcgataatat
     1081 gatttccgtt tacctaatat acgaaacttt tctgattcat aaagttttct tgctgcttca
     1141 ttagatagac gtacttctaa aaaaacatgc agcgaatctt ttatttgttc cgtaatcagt
     1201 aattgtaaca aatgccgtgc atgccctttt ccttgttctg aaactgcaac agcaatattt
     1261 gtgatctcga cttcatctat tactttcgag tatccaataa agccacagat ctggttatct
     1321 tcagctaaaa gcagataatc agagtgaggc tgttggatgt ctgccaaaaa ctgcattttc
     1381 gtccaaggtg aaccatgtgg ataagcattt tcgctaatca tccatacgca ttctgccaag
     1441 tacgcttcat caaaatcttt ttttctgcaa atcaactaat ctcactcatt ttctaaaagt
     1501 tttatcatat ccaacgcatc ttcttttgtt tcagtgtaat atcttttttt gatcgttgtt
     1561 gcttgaaacc ccaatttccg ataaagtctt tgtgcatctt gattactcat acgcacttct
     1621 aatgacatcg tctcacagcg attcattata gcaaagttct cgatttcgtc aataagcagc
     1681 gaaccgattc gtttacgctg atatgccggt cgaacggcaa tattcgtgat atggcaatct
     1741 aaaccgaacg ctcttgaacc gataaaacca atcattcttc cattatcaaa gatacctaga
     1801 tatagatgag gaagcggtga cttcaattct gatataaagg cactttttgt ccatggcaat
     1861 tcgccattat agacttctcg ttcgacatcc agcagctctt tgatatcttc tgtagtcaat
     1921 tctcttagta cgtaacgacg atcgatttgt tcgactgttt tttctgtata aatcatttta
     1981 tcttgaaaaa agcttctggc atacgctcta aattttttca acataacttt cgtctccagg
     2041 tgtgtgcgtc tccaaccatt tttcttctgc ttctactcgt tttaaatagc gaggaagaaa
     2101 agcttgtttc tctgcaactg gttcaactaa agcacctaat tgagccacag ttacaccact
     2161 tggaatatcc catgctggaa ctgtattaat catcgcacta ggcaatattt cctttatttc
     2221 agagaagaat ttgtgacaat cactcccgac gaaataaata gcttcattca gcttttgtag
     2281 ttgtgtgaac aaatcagaag cagaaatatg tacatcttca agtacagttt ctaatctatt
     2341 ttctttccag cggtatgccc cagcgtatac attttttctt cgagcatcaa acatgggcac
     2401 aataatatta tccacaccga tgcaattagc cgcgatggtt ttcagactag atactcctac
     2461 tagctcttta tctaatgtat aagcaatcgt ttttgctgtc gttacaccta tacgaagccc
     2521 tgtataagaa ccgggaccat cagaaaccgc gattcggtca atgtcttttg gacttatttg
     2581 aagatctgca aacaattggt cgattgcagg catcagtgtt acactgtggt tgcgtttgat
     2641 atttgtttgt atttgtccaa gaatttgttc gtcttctacc acaccgatcg ccaaagtttg
     2701 gtttgcagta tctattccta atgtaatcat atatttgatc ctttctgcta tgacattctc
     2761 gccttccatt gtagcatact tttttattct gaggatgaaa ataccaaagg caaaactagt
     2821 acaataatac atgattatct gctggcgggc aaattttgcc ccttcctgaa acacaaaaaa
     2881 gctgcggtat cactccattt tcccacagct tttccatctt ttatttcgtt gattctcgaa
     2941 taatcaactc tgtttccaca ataatctttc ttgaaaccgg tgctttggaa ttacttaatt
     3001 gaagaatcgt ctccactgct agctctccca tccattcggt ataaactttt acagtagaaa
     3061 gcgagggcgt tacatatttc gctacactga catcattgaa gcccatcaca gaaatatctt
     3121 ctggtaccac taaacctttt tcttgaatcg ctttcagtgc gccaatcgct aatgcatcat
     3181 tagctgcaaa aagcgcatca ggaacatgcg gattcgtttt taaatactgc ttcactgctt
     3241 gatatcctga ctcgacagaa aaatctgctt cgataattgg accttcgtcg tagccttttt
     3301 ctgccaaaac ttgttgaaat gccataattc tgggatcttt tagccgctga tggtttttct
     3361 tcgtatattc tgttccagca agcatagcga tatgttgcgc tttatgggag acaaagtgtt
     3421 caatgacttt ttgaacactt gacttaaagt cgacaactag agagtcgatt ccttgagaag
     3481 tgccgtcaga atctaccaaa agcaaggcat tttgggatcc tttcatttct aataactgag
     3541 cttcgtcgaa ttttcctagt gcgatcgttc catctgccgg aagatcacta atggaatccc
     3601 acgtttcttt caataaagta acgtttgctt cttctgcctt tttttcgatt cctaggcgaa
     3661 tagataaata ataaagatct tcaagctctt ccgtatcgtc gtaccactgg atcaggcgaa
     3721 gcgtttgctt agtatttgct gcatttttac gatgtttcgt ataatttaag cgttctgctg
     3781 cttcaaaaat tttttgcttt gtggctaagc caacggaaag atcaggatca tagttgagaa
     3841 ctcttgaaac agtagcagga gaaactcctg ccaactgcgc aatgtcttta attgttgcca
     3901 tttgtctctc ctcctttttt acgtttataa aatcgcagta aatcgttcaa acgctgcctg
     3961 tcctttttca tctcgcttga atactccagc gtcttccagt acacgtgcaa aaacctgacc
     4021 tacagatttt tgtacgacat cggtgaccgt atctttcgtc aaatcaggat gttctttttt
     4081 taattgttct gcccacggac gatgatattc agccatctgg ttttcttctc ctaataagaa
     4141 atgctctact tctactagtt ctggtttcaa acgaggtggt aaaacagcaa gccccattac
     4201 ttcgatcaac ccaatgttct cctttttgat atgttggaca tctttatgtg gatgaaaaat
     4261 cccatcagga tgttcttctg aaacattgtt gtctcgaagg acaaggtcca actcaaagta
     4321 attgtccctt cttcgtgcaa ttggcgtaat cgtatggtgt ggtgtaccat ctgctgaaaa
     4381 agctctgata gatactgttt catccgaata tgctttccat ttttccaata ctgagttagc
     4441 tgcttcaata agcaagtttt tctctgttga ttgcaaacgg atcacagaca tcggccattt
     4501 cacgactcct gcgatgactt gcggataatc ttctagtttg ataagttttt ctatcggtgc
     4561 ttttgccata ggaaattcat gacgtcctgc ctgataatga tcatgagaaa gaatcgatcc
     4621 tccaacaatt ggtaagtcag cattcgaacc aacaaagtaa tgaggcaaga cttcagtaat
     4681 ccgaagcagt ctagcaaatg tttcctttgt gattttcatc gggcggtgtt gttctgataa
     4741 aatgatcgaa tgttcattgt aataagcata aggggaatat tggaatcccc agctttcacc
     4801 gtctagattc attcgaataa tccggtgatt ggtgcgggct ggatagttca agcgacctct
     4861 atatccttcg ttttccatgc aaagcatgca ggtaggatag tctactttta ccgcctcgcg
     4921 ttctgctgcg atttgttttg gatctttttc tggcttagat aaattgattg tgatttccag
     4981 ttcaccatac tccgactctg ccggaaattt gatgtttttt gcgatggctc ttgttttgat
     5041 gtagtcattt ttcttactta aattaaagaa atactcagtt gcttcctctg ggcttttcga
     5101 atagtgctgt gcaaagaatg cattgacgac tgaaggtggc ggtgtgagaa aatccattaa
     5161 ttgcgcttca aaaatctctt gttctgctgg caattctgaa agaacaccat tttctcttgc
     5221 cttagcgaca agctgatcca ataagtctac agattcttcc ttaactggtc ttacttcaat
     5281 gctttctaga gcatcttctc ctaccatccc taaaagacga ttctgtaaat ataagcggtc
     5341 catttccatc catccgccag cttcgatcgc aagtgtggca aaatcacaaa tcgtttgact
     5401 gattgacata tctcattccc ctattcttca taaccatttg gatggctgac atgccaatcc
     5461 catgctgttt tgataatcgc tttgatatct gtgtattcag gtttccagcc cagaatctct
     5521 ctcgcttttt cacttgaagc gactaatcgg ctaggatctc cagctcttct tggtgctatt
     5581 tctgctggaa tttcttttcc tgtcacttct ctagctgctt ctagcatttc cttgactgag
     5641 tatcctttgc tgctgcctaa attgaagaag ttgctttcat ttccttcttt caagtattcc
     5701 aaagctaaaa tatgtgcagc aatcaaatct tctacttgaa cataatctct gatacatgtt
     5761 ccatctggtg tatcataatc gtctccataa acagccaagg cttttctttg accaagagct
     5821 acttgtaaaa tgattgggac aagatgcgtt tctggtgtat gatcttctcc gatagatgca
     5881 tctgcttttg ctccggctac attgaagtag cgcaaagcga catagcgcat gccatatgct
     5941 tggtcacacc acttcatcat tttttccatc atcaatttac tttcaccata cggattttta
     6001 ggattagctg gtgtatcttc tgtgattgga gactctttcg gctctccata agttgctgca
     6061 gtagaagaga atacgatttt attcacgttg aactcgtgca tgacttcaag taagatttgc
     6121 ataccgtata cgttgttgtt gaaatacatc aatggttttt cgacagattc tcctactaaa
     6181 gagcttgcag caaaatggat tactccttca atcggttcct tttcaaatac ggaacgtaga
     6241 aattctttat cccggatgtc tccttcataa aaatgagcat ctggatgtac tgcttgtttg
     6301 tgacctgtga gcaaattatc taccacagct acttgatacc ccttttgaac tagctggtct
     6361 acagcgtgcg aaccgatata accggctccg cctagtacta aaattgtcat aaaaaacact
     6421 cctttgatta gtaaaattgt gtttgttgat tctat
//
LOCUS       DACEMO010000146         6327 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEMO010000146 DACEMO010000000
VERSION     DACEMO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.151"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(51..1469)
                     /gene="nhaC"
                     /locus_tag="IW728_002158"
     CDS             complement(51..1469)
                     /gene="nhaC"
                     /locus_tag="IW728_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1025501.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1872..3095)
                     /locus_tag="IW728_002159"
     CDS             complement(1872..3095)
                     /locus_tag="IW728_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1025502.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3126..3965)
                     /locus_tag="IW728_002160"
     CDS             complement(3126..3965)
                     /locus_tag="IW728_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1025503.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(3982..4662)
                     /locus_tag="IW728_002161"
     CDS             complement(3982..4662)
                     /locus_tag="IW728_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1025504.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4664..5737)
                     /locus_tag="IW728_002162"
     CDS             complement(4664..5737)
                     /locus_tag="IW728_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1025505.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
BASE COUNT         1821 a         1389 c         1077 g         2040 t
ORIGIN      
        1 tcgtaaaagg gattgtgaca aaagtcttgt cacaatccct ttttatgttt ttattgttct
       61 aattgcgctg tcttgcttac tgcttcttgc ggtttcacaa atttttttga gattccccaa
      121 cctgtgaagg cactgaccat cgaaaacaca ggagaaagca aactgaaaaa tacgaatggc
      181 aagtaagtga gtgtaggaat accaaatgta cttgctacga aacttccggc aattccccaa
      241 ggcactaagt agttgattac tgttccacca tcttctagcg ttctacctaa tacagttgga
      301 tcaaatcctg ctttttcata agtttcttta aatgcattac caggcagaat aacagatagg
      361 aattgttcac caataaaaat atttactccg atacttgtca tcaatgttgt aaaaatcaaa
      421 tttggtgtgt tcgtcatctt tcttgatact cgttccatca cggttgagat caagccaaat
      481 tcgatcaaca atccacctaa tgacaaagtt agaataatca atgacacagt aggcatcata
      541 ctttcgattc ctccgcggct aagcagtgaa tcgatttgtt ggtttccagt atttgaaaca
      601 aaccctgatt cgatgacccc agccaacttc gttaaggaag tttgtgggtt ttgaataaaa
      661 atcattccta ctgctacagc gatattcaac aaaatcgtta aaattgccgg cacctttttc
      721 catgcacaca gtaacattaa aaataaagga atcagtgacc agattgaaat agtaaagttt
      781 gcttctagaa catcagtcac ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt
      841 cctaaaatca tgaataaaaa gaaagaaaca acaaatgccg ggacagttga ccacatcata
      901 tgcttgatgt gtttgaacaa atcggcatca acaatagctg ctgctagatt tgttgattcg
      961 gataatggag acatcttatc tccaaaaacg gcacctgaaa caatagctcc aacaaccagc
     1021 gctggattta agcctagtgt tgtcccaata ccgaaaaatg caatcccaat cgttgacata
     1081 acagtaaagg cactccctac agcgctacct acgattgcac acacaacaaa tacagaaggg
     1141 acaaaccact ggacactgat caatttgaac ccaatgacca ttagagttgg aatgatccct
     1201 gcttgtatcc atacagcaat caatgaaccg actaaaataa aaataaagat tgggatgatt
     1261 cctggtttga tgccattgat gattccttcg tgcagccaat ctgtcgggta tctttttatc
     1321 actgcataca acatgaccat tccgatcgct gctaagatcg tgatattagg tgagaccttt
     1381 aatccgacaa cgccaatcgc aatgcctaaa acaataacca ttaatacaag tgttgcttct
     1441 aaaaaacttg gtgttccttt ttttcccatg ataatctctc cttttttagt tgttcttatg
     1501 ttctaaaaaa gttaagatat cggattactc cgccgcaatt catccgcgat tcctccttta
     1561 taaaaataaa aagacctgtt ttaaactaag gacgaatgac tccgcggtac caccttaatt
     1621 aaaaacagat ctgtttttca ctttcaaact tttattcaaa tgtatggact ttccatgtaa
     1681 ttcgtcaatt aggcttgatc ggttcacagc gaccaccgac tttctgaaca aaacacctaa
     1741 ccgctactgc gtgaaaagtt gacttgtacg aagtttacct tccataagat catttgtcaa
     1801 tgaaaaagaa aatatttttt catttttctg aaaattattt ttataacttt cacacacaag
     1861 tttatttttt actagaaagt agcgccctct tgtttttttc ttgctactat ttcttgatgt
     1921 tcttgcttga ttctttctaa tagctttggc tctttcaaca gatcaagagc tgtattggcg
     1981 agtagttctg ctgcaatcgc aatagaagca aggcctttgt cacttttagc agcagctttg
     2041 aattcttctg tgtgtccggc aatatatgtc tcagagattg ctaccgtagg ctggattgtt
     2101 ggtatgactt ggctgacatt tcctacatca gaagaaccga gacttgttct ttgttccgtt
     2161 tctatttctt catctggaac acctgcttct tttgcatgct ggaagaataa gtcatcaaaa
     2221 gaaggagtaa cgatgacatc atcaactgca ttttggaaca atccaaactc aaaagtcgtg
     2281 cccgtactta tcgcagcacc tcgtacgata ttttccactt tttcatatac tttatctaat
     2341 gtttgccgat ttcctgcacg taaatagaaa cgtccagcag cgtaatctgg aatgacgttg
     2401 gcagctactc ctccgtctgt aatgatccca tggatattga catcttttgg caattgcaag
     2461 cgtagagcgt tgattccatt aaacacttgg atcaatgctt ccaaagcatt tactcctttt
     2521 tcaggtgctg ctgctgcatg ggaagcgaca ccgtaaaatt tgatgtcgac tggatcattc
     2581 gctaaagatt ctgtcgtttt cccataacga aaagctggat ggacgcataa cgctacatcg
     2641 acatcctcaa aaaaaccttc tcgaacaaag ctgcctttag ctgatccgtt ctcgccccct
     2701 tcttcccctg gggtgccata tacacggatt tctccaccag tttcatcaat gaattgcttc
     2761 actatgctgg cggctaatac ggagtaagta ccaaacaaat tatgcccgca tgcatgacca
     2821 atacctggta aagcatcgaa ttctgccaaa aaggcaattg tgggaccggg tttattggaa
     2881 atataacgtg catcaaaggc tgttcggtgt ccagctactt ctttttctac ccaaaacctt
     2941 tctttttcta attgtttaat caacgctgct gaggaataaa actcatagtt acttacttca
     3001 ggatgttcgt ggatatctag agcaagtgct tgatattctg gtaaacgata ttggattaga
     3061 tccttcaatt ggtttgttga gattgttgtt gtcataaaaa attcctccta tgctagtcat
     3121 ttttcttatt ttctcccgaa tgtttcccat gcaggcacgc ttgatccttt tgatgttttc
     3181 tcgattactt tgactgtgtc ttctgtttga taagcgtcta ctattttttg atacgtttta
     3241 ttgttttcat cttcttttct tgcaacgatg atattcacat aaggacgtgc atggtcatcc
     3301 actggttcta agaagatggc atcagaagca ggattaaatc cagcatctac agctacaccg
     3361 ctattgatca ctgaaatgtc tacatctgac aaagcacgtg ctgtttggga agcatctaat
     3421 tcagaaatgt ctagattttt tctattctcg gtaatatcag aaacagtggg cgcttgtcct
     3481 tttgccttat ccactttgat caatccagca gtttgcaata acagaagagc tctgccgcca
     3541 tttgtaacat cattagggat tgctactttt gctccgtctt tgatatcagc tacatccttc
     3601 aaagtgttag aataaattcc cagcggtgca ttgacagtat ttccgatcga tactaaatca
     3661 gttccgtgtt ctttattgta attgtctaaa aagaattgat gttggaaaga attcaaatca
     3721 atcgatccat ctgctaaagc cacatttggt tgattgtaat ctgtaaattt cacgtattcc
     3781 aaattaatgt tctccttttt caaacggtct ttcacgttgt cccatacatc tgtatcttct
     3841 ccaataatcc ctagttttac gatagtttct tttttgtcag ttgtatctgc ttttccttgg
     3901 ctacaccctg ccaaccaaat aattgctaca aatgtaatga ctacaaaacg aataattttt
     3961 ttcatttttt ctcctccact ttcaatgtgt aattttttta atcagttgat tactgataaa
     4021 ttgacttaaa aaaactaaga ttagaatcaa taatgtagcc acaatcgtta catctgtttg
     4081 gaatcgatta taccctctcg atatagcaag attccctaat ccgcctgcac ctactgctcc
     4141 agccatggta gtcagtccga tcacgttgat gatagtcaac gccgagaccc gaatgatacc
     4201 aggcaaacct tctaacaaat acacacgtaa aatgataccg attggactgg ttcccatcgc
     4261 ttctgccgct tcgatcaccc caggatcaac ttctagcagt gcgttctcga tttgacgagc
     4321 aaaaaacgga acaactccaa aaatcaatgg cactaaagct gctttcgcac caatcgtcgt
     4381 tccaactaag aaacgagtag tcaatgctaa aagagaaagc aagataatga aagggatgga
     4441 acgaatgata ttgatcagct tatcaagtat ttcaaatagc agacggttag ctagtaatcc
     4501 attagggcgt gtcactacta acgtcacacc taagatgatt ccaatcatcc cgccaatgaa
     4561 tgctgtccaa aaagacatat atagtgtttg cttcgtcgct tcgacaaatt catccggaat
     4621 caaataaaca ttgggcaaat atgttttaaa gaaatcagtc attttatctt cctccttcga
     4681 tcactttgat acgactatct aaatcgtttt tttgtaaaat atttattttt acaccagatt
     4741 tgatcaaata atccaatgct tttttccgct gtttggtttc tccagctaaa gtaacgatca
     4801 aactccctaa cggcgtttct tgtaataact ctacattgcc atataaaata ttcgctgtta
     4861 cttgatattt gctgtacaga tgagcgatta gaggttcgtt cgtctgattt ccaatataac
     4921 ttaattccac cagccattta ttggcgatct gatcggcaaa agcagaatgg gcaatgatcg
     4981 tttctaatgc ttggtcaaga tgagtagctg ttcgaataaa atctttggtc aattcttctt
     5041 caggattgct gaagatctgg atgctgctac ctttttcaac gattcttcca tcttccataa
     5101 ccgctacctt gttgcagatt tctttgacaa cttgcatctc atgagtgatc aggacgattg
     5161 ttaaacctaa ctggcgattc aattttttta acaatgcaag gatctgaagg gtcgttttcg
     5221 gatcaagtgc actggttgct tcatcacata ataatacctt aggttcattc gccaaagctc
     5281 gtgcgattgc tactcgttgt ttttgtcccc ccgacagttg tttaggataa gcagatgctt
     5341 tttcttccaa accgacaagt tccagtagtt cattcacttt ttgacgacgt tcctgtttcg
     5401 attttccaga atactttaaa gaaaaatcaa cgttgtcaaa aatagttcgg ctttccatta
     5461 gattgaaatg ctgaaagatc ataccgatgg cctttctttc ttttcgcaat tctttgggag
     5521 agagctgact cagattcgtc tgattgacaa tgacttctcc tgctgttggt ttctgcagca
     5581 aattcatcac acgaactaac gtacttttcc ctgcacctga gtaaccgacg attccataaa
     5641 catcgccttt ttcaatcgtt aaatctacag aatctactgc ctgtatctct ttttcttttt
     5701 gtttgaaaca tacagaaaca tctcgcaatt caatcattaa aattcctcct tatctaaaaa
     5761 aagcgtcctc aagaaaatga aaatgtcatt ttcttgagga cgcttttttg cgtgttacca
     5821 cctcatgttc aagctgtgtt cacacacaac tcctcttcaa gtacgatcat actctagcaa
     5881 gatatcggtc gcagccgtca tcgccttaca tctatgctcg ggatgaattg ctcgatggcc
     5941 attttccaat agtcgttcct tgcttcttct cagctaccga agctctctgt caaaggttct
     6001 tctatcgtac ttaccatttc aatgcaaata atattcaaaa aaaaagtcct tttactaaca
     6061 taaactatgt cagtaaaagg acgacgtatc gcggtaccac cttttttcgg aagtttcctt
     6121 ccctcaagcc caacatctca aaaagagaga ttggcgttcg ttttaacggt cgaacagttc
     6181 cgaatttgcc tacttatcga caaattattc tccaaggcca ttttcagctt ttcttacttg
     6241 actcttctca ccagccagag cctctctgca aagtttccaa acttactttc cttttcaacg
     6301 aaatcgatat tttgttgttg ggattaa
//
LOCUS       DACEMO010000147         6303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEMO010000147 DACEMO010000000
VERSION     DACEMO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.152"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            35..1666
                     /locus_tag="IW728_002163"
     CDS             35..1666
                     /locus_tag="IW728_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1025506.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSNIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1669..2748
                     /gene="uxuA"
                     /locus_tag="IW728_002164"
     CDS             1669..2748
                     /gene="uxuA"
                     /locus_tag="IW728_002164"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1025507.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLQTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2822..4249
                     /gene="uxaC"
                     /locus_tag="IW728_002165"
     CDS             2822..4249
                     /gene="uxaC"
                     /locus_tag="IW728_002165"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1025508.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLGYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4342..4989
                     /gene="eda"
                     /locus_tag="IW728_002166"
     CDS             4342..4989
                     /gene="eda"
                     /locus_tag="IW728_002166"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1025509.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSLENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5109..>6303
                     /locus_tag="IW728_002167"
     CDS             5109..>6303
                     /locus_tag="IW728_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1025510.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLIIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQFKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSI"
BASE COUNT         2137 a          842 c         1314 g         2010 t
ORIGIN      
        1 tcaaaattaa catattagtt taaggaggtt aattatggtt tcgattaaaa attggcaaaa
       61 ttctatcgaa gaattcaatg aaatgcagat tctactgccg tgttttgatg tcaaagaagt
      121 taagcaagaa ggccagcaac atccagtatg gcttcatttt ggtggaggaa atctttatcg
      181 aggatttcat gcagaaattg ctcaatgctt agcagatcaa aatgaattag aaacaggagt
      241 agttgtgtgt gaaacatttg atgaagaagt agtggaaaaa gcttatcatg actacaacaa
      301 tgacattttg gaaattgtta tgagtggaga tggtgaactc aaaaaaaggc ttttggcagt
      361 aacggctgat agtttatatt gcaatccgaa gaatatagcg gactatcaaa gagtggttaa
      421 atattttgaa gatcctaaat tacagtttac aacgtttact attactgaaa aaggttacag
      481 tttaaaagat tctaaagggc aattcttttc ggtagtcaaa gctgatatca aatctggacc
      541 tagcaatcct caacatacga tgagtattgt aacatccttg ttatatagca gatttaatgc
      601 tggagagttg ccgattgcga tggtttctac tgataatttc tcacagaatg gtcaacgttt
      661 tcaagatagt atttttacga ttgcagaagc ttggcaaaaa gaaggatatg tttctgaaaa
      721 atttattgct tatttaaagg ataaagaaaa agtgactttt ccttggagta tgattgatcg
      781 aattacgcca aatccttcag agaatgtaaa ggaagatcta ttgatgatgg ggctcgagga
      841 tttagatatc attcacacaa aaaaacatac caatattgct gctttcacta atactgaggt
      901 agttcattat ttagttattg aagatagttt tccaaatggc cggcctaaat tggaaaaggc
      961 tggtgtgatt atgacagata gagagacggt tgataaagca gacatcatga aggttacgac
     1021 aagcttgaat ccacttcata ctgctttggc agtaacaggt tgtctgctag gatatcaatc
     1081 aatttcagca gagatgcagg atgatgattt ggttggttta atcaaaggaa taggttattt
     1141 agaaggttta ccagtagttg aaaatccagg cattattgat ccaaaacaat ttattgatga
     1201 agtaattaat aaacgtttgc ctaatcccta tattccagat acaccacaac gaattgcaac
     1261 agatacttct caaaaagtag cgattcgcta tggtgaaaca attaaaaagt atgtcgaaaa
     1321 agacggaaat gcagaaaatc taacatttat tcctatcgct attgctgctt ggttacgtta
     1381 tttactggca gtcgatgatc aaggggaaaa ttttgtgcca agtcctgatc cgttattggt
     1441 agagttgcag gataaattat cagtattgaa attaggtgaa caagatcctt caaatattca
     1501 ttgtgcggta caggaaattt tagaaaataa agaaattttt ggtttagatt tatatcaagt
     1561 tggggtagga aagaaaactg aaaaaatctt ttgcgaaatg ctagtaggtc ctcaagcagt
     1621 tcgtaaaaca atcaataaat atataacaga agttggagga aagtaactat ggaaatgact
     1681 tttagatggt atggggcaaa tgaagaaaaa atctcactac aagaaattcg tcagatccca
     1741 gcaatgaaag gaattgttgg aacattaatg gatataccag ccggagaggt ttggccgaga
     1801 gaacgaatta aatctttaaa agatgaagtc gaagccaatg gacttacctt gaaagttatt
     1861 gagagtgtta atgttcatga tgatatcaaa attggtttac aaacacgaga tcaatacatt
     1921 gagaattata aacagacgat tcgcaatttg gcagaatttg ggattgaggt tatctgttat
     1981 aattttatgc cagtatttga ttgggtaaaa tcggatttgg attatcgttt agatgatggg
     2041 tcttctacgt tagcctatat ttcaaaaaat atccctactg atccggagga aattattaga
     2101 aaagtagaaa attctactaa taattttgaa ttgccgggtt gggaacctca tcggttatca
     2161 caaattaagc aattattaga agcctataaa gaagttgatg aagagaaatt acgagagaac
     2221 tttgcatatt ttctcaaagc aattatcccg acatgtgaag aagttgggat taaaatggca
     2281 gttcacccag atgatccacc atattccatt ttcggattac ctcgtattgt taaaaatcgt
     2341 gacgatttag attggttgtg taatgtagtg gattctcctt ctaatggtat tacattatgt
     2401 actggaagta ttgcagaacg accagataat gatgtctatg aaattctatc tgaattttgt
     2461 caacgggatc ggattccttt tgcacatgtt cgtaatatca aatatttaag tgacaatgga
     2521 ctcgattttt acgaagcgcc gcataaatca gaatatggtt ctctagatat gtataagata
     2581 ttaaaaacaa tgtacgataa tggatttaat ggctatattc gtccagatca tggtcggatg
     2641 atttggggag aaaccggtag acctggttat ggattatatg accgagctct aggtgcagtt
     2701 tatttaaatg gtatttggga agcgcttgaa aagggggcga gtaaataatt atataatgga
     2761 gtttatttag aggaaaaatt ttctcttcta aaaactaacg gaaggaaagg agtgtttaaa
     2821 tatgttttta gataagaact ttttattaga gaatgaaata gctaaaagac tttttcataa
     2881 atttgcaaaa caccaaccaa ttatcgacta tcattgtcat ttagacccag catatttgct
     2941 agaaaataag caatacaaga atttgactca ggtatggtta aatgataaag gagctggaga
     3001 tcattataaa tggcgattga tgcgagctaa tggtatagaa gaagagttga tcagtggtcc
     3061 aggtgatgat tatgaaaaat ttttagcttt tgtaaaaaca gtggagaagg cgccaggtaa
     3121 tcctattttt gaatggtctc atttggaatt acaacgttac tttgatattg atttgacaat
     3181 ttctgagaag aatgcaccag aaatttggcg acgtgctaac gaaaaaatcc aaacggattc
     3241 ctatcgcccg aaagaattgt tggagatgat ggatgtgaaa gccatctgta caacggatga
     3301 tcctacagat gacttggggt atcatactga gctttctaaa caggataatt ctttcaaggt
     3361 attacctact ttccgtccag ataaggtctg gagtatcaaa tcatcaggct tttcgaacta
     3421 tattttaaaa cttagtcaag cagcggctat accaatcaaa gactttaatg ggttgaagca
     3481 agcattgagc caacgagtgg atcattttca tcaaattggc tgtcgattag cagatcacgg
     3541 tttgaatact tatttttatg aaatagcaga tgaaaaaata ctcgacaatc tgttaataaa
     3601 agctttagca ggaaaaacgg atttccattt atcagaaatt gcacaatttg ttacaggtat
     3661 tcaattacat ttgatgtctg agtatacaag atatggttgg acgatgcaaa tgcatttaaa
     3721 tgctttaaga aataattcaa gtatattaaa ggaacaaatt ggagtagatg tcggtggtga
     3781 ttcgatgggg gatcaagcta gtttgacaca aaatgttgta gcattgttcg ataccgctga
     3841 aaagagtggg tgcttaccaa aaacaatttt atattcatta aatccaactg attggcttcc
     3901 actagcaaca gcaatgcaaa gcttccaagg gggtatgatt caaaaaatcc aattgggatg
     3961 tgcttggtgg ttcaatgata cagctgaagg aatgcgtcaa cagttgacta cgatggcgca
     4021 acaaagtttg ttgtcgaatt ttacaggtat gttgactgat tctcgaagtt ttctaagcta
     4081 tcctcgccat gagtattttc gtcgagtatt atgtcaatta ctaagcgagt gggttaaagc
     4141 aggccgaatt ccagatgatg aaatgtatct tagtcagata gtggagaata tttgttatcg
     4201 taatgcgtat aattatttta atttttttga aatcggaagt agaaaataat acttgaaata
     4261 aaagcttaaa tttattcttt ttaagttagt acagctagac aaagtatgta tccataaaag
     4321 aaggagatag aggagataga tatgaaaaaa gtagaaattt taagtaagtt agaagcggct
     4381 ggagtgattg cggttgtacg tgggaaaaat aaggatgagg ccattaaagc aataaattca
     4441 attattgcgg gtggaattaa agggattgaa ctaactttta cagttcctaa tgctgaagaa
     4501 ataatcaaag aaattgctaa aaaatatgct gatcaaaaag atgtaataat cggagccggt
     4561 acggttttag atgcgtttac tgctcgtatc gcaattatgg ctggggcaga atatatcgtt
     4621 agtccatctt ttgatcgaca aacagctgag atttgtaatt tatatcaaat tccttattta
     4681 ccaggttgta tgacaattac tgagataaag gaggcactta ctgctggcgt agatatcatt
     4741 aaactattcc caggttctgc ttttggtcca tccattgtaa aagcctatca agctccatta
     4801 ccacacgtaa atataatgcc tactggcgga gtaagtctag agaacttaga agattggttt
     4861 gaagtaggag ttgttgcagt tggtgtaggt gggaatttat tggcttctgt tgaaaaaggt
     4921 gattttgaag aagtaagtcg agtagctagt aaatatatag ataagtttta tgagataaag
     4981 aattattagg agaaaaaaca aataatactc tgaaatgtaa aaaataaata ctgactcttt
     5041 acgcttcaga gtatagtacg tactttcata agaatgaaaa cgcttaacaa aagaagggag
     5101 agaattgtat ggaaagaaaa tttggtataa gagacaaaat aggatatatg tttggagatt
     5161 ttggaaacga ttttactttc atttttgcta gttctttttt aatgatcttt tatacaaaag
     5221 tattggggat aagcggagct gccgttggaa cattattttt agtagcaaga tttatcgatg
     5281 cagttactga tgtaactatg gggcgtattg ttgatagatc gaaagcaact aagcagggta
     5341 agtttaaacc gtggatttta agaatgagtg gtccagtcgc tttagcaagt tttttaatgt
     5401 atcaaacttc tatgatttca gcttctatga gcgttaaggt agtatacatg tatgttacgt
     5461 atatattatg ggggagtatt ttttacacat ctataaatat tccttatgga tcaatggcat
     5521 cagctattac ttctattaca gaagaaagaa caatgctatc gacattccga aatgttggag
     5581 ctactttagc aagtttgatc attggtgtat taacaccatt atttatttat acaagagatg
     5641 attcaggcgc tcaaatagtt cgtggtggat caacttttac aattgttgcg ggaatatttt
     5701 cgatatgtgc acttatttgt tatttaatat gttataaact aactactgaa cgagtaagaa
     5761 ttgaccaatc tcaattcaaa gtttcattgt taaaatcttt aaaagggtta gttaaaaata
     5821 gggcattact ttctataatt ggtgcggcta ttttcttact ccttgctcaa ttattaatta
     5881 tgagtatgaa taattatgta tttcctgatt actataatga tgcacaggga atagttttaa
     5941 tgaattttat caatcctatt ttggttttaa tagttgtttc acctttaact ttgtttttaa
     6001 gtaaaaaata tggaaaaaaa gaactggcat cagtaggaat gttcttttca gcaatagtat
     6061 atttgatctt atttttgata aaaactagta atatgtatgt attcttaatt cttagttcgg
     6121 ttggctatat gggtttgggt gtgtttaata ctgttatttg ggcaaatatt actgatgtta
     6181 ttgatgatca agaagtaaag tctgatcaaa gagaagatgg gaccgtatat gcggtatatt
     6241 cttttgctag aaaaataggt caagctctag ctggaggcgt cggaggttgg actctttcta
     6301 tta
//
LOCUS       DACEMO010000148         6261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEMO010000148 DACEMO010000000
VERSION     DACEMO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.153"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(53..241)
                     /locus_tag="IW728_002168"
     CDS             complement(53..241)
                     /locus_tag="IW728_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1025511.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            511..873
                     /locus_tag="IW728_002169"
     CDS             511..873
                     /locus_tag="IW728_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1025512.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            925..2607
                     /locus_tag="IW728_002170"
     CDS             925..2607
                     /locus_tag="IW728_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1025513.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2724..4760
                     /gene="recG"
                     /locus_tag="IW728_002171"
     CDS             2724..4760
                     /gene="recG"
                     /locus_tag="IW728_002171"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1025514.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4809..5810
                     /gene="plsX"
                     /locus_tag="IW728_002172"
     CDS             4809..5810
                     /gene="plsX"
                     /locus_tag="IW728_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1025515.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5932..6174
                     /gene="acpP"
                     /locus_tag="IW728_002173"
     CDS             5932..6174
                     /gene="acpP"
                     /locus_tag="IW728_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1025516.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2081 a         1088 c         1426 g         1666 t
ORIGIN      
        1 ttttattaaa taaaaaaccg ttcactatct gaacggtcca agtatcattc aattaaacgc
       61 gttcaacttt tcctgatttc aaagcacgag ttgacaccca aactttttta ggttttccgt
      121 cgaccataac gcgaactttt tgtaagttag gtttaacagt acgttttgtt gagttcatag
      181 cgtgtgaacg gttgtttcca ctttttgtct tgcgacctgt aaagtaacaa acttttgcca
      241 tggtaatttc ctcctttgcc ttgatttgga gcaatagaat aattcagctc ataccagact
      301 aacatatcat atagaatggg aatttgcaag tacattttca tagatacaat tgacaattga
      361 gaagaataat tgtaaattaa acaaggttat acctgaaatt ttgcaaggtt tttgagtact
      421 ttgggtaact gccttttatt tcaagttgcg ctatgataaa atatgataga agaaaaatgg
      481 gagtttttta cccgttaagg aggctcattc atggctgtaa aaattcaaac gtcagctgga
      541 accattgaaa ttaccaatga tgtaattgca acagttgttg gtggcgccgc gacagatgtg
      601 ttcggtgttg taggaatggc cagcaaaaat caaattaaag ataatattac tgagattttg
      661 cgtaaagaaa attactcaag aggagtagtt gtccgccaag aagataatgg tattgctgta
      721 gatgtttata cgattatcag ctatggtacg aaaatctctg aagtttcccg caacgtccaa
      781 gaaaaagtga aatacaatct agaggcatta ttaggcgtaa ctgccaattc agttaatgtc
      841 tttgtacaag gtgttcgtgt actgccagac taggcaggag actggctggt aactcagcag
      901 tcgatctgaa ggaggattat ctaggtgagt gtaacagaaa taagtgcaag ccagttccag
      961 gaaatggtcc aggcaggtgc gaatcgtctg caagtaaatg cagaatacgt caattcatta
     1021 aatgtattcc ctgtgccaga tggcgatacg ggaacaaata tgaatctatc tatgactagt
     1081 ggagctaagg cagtagtaga ttcaacctca gaaaaagtag gagaattagc agcaatgctt
     1141 tcaaaaggat tgctgatggg tgcgcgcgga aattccggcg ttattttgtc tcaattgttt
     1201 cgtggatttt ctaagcaaat tccagatgtt gtctcattaa atgcttctga tcttgctgca
     1261 gccttcactc atggagttga aactgcgtat aaagcagtaa tgaaaccagt agaaggaacg
     1321 attttgacag tagctagaga agcagcgaaa gctggagaaa agaaagcaaa aagtactgat
     1381 gatgtcgttg aagtaatgac agctgttgta aaaggagcaa agcgtgcttt agctaaaact
     1441 ccagacttgc ttccagtcct aaaagaagtc ggtgtcgtag acagtggagg gcaaggactg
     1501 ctgtttgttt atgagggctt tttaagtgca ttgaatggag aattccaagc ggatgaaaca
     1561 tatgaacctt ctccagcaga aatggatgat atggtcaatg cagaacatca tcgcagtgtc
     1621 caaggtcaaa tggcgacaga agatatccat tttggttact gtacagaaat catggtaaaa
     1681 atcggagaag gacctacagt cgatagtaca ttcgattacg aagaattccg taactattta
     1741 gatggtatag gagattcttt acttgtggtc aatgatgacg agatcatcaa agtccacgtg
     1801 catacagaaa acccaggaga agtcttgaat tatggacaaa aattcggttc attagtcaaa
     1861 atcaaagtcg acaacatgcg cttgcagcat gagacgattt tggaacacga tgaagaagtg
     1921 aacgagtttg cgaaaaaaga aataccacgt atcccgtatg gtatcgtagc gatcgctgct
     1981 ggtaaaggcg tccaagagct gtttgaaagt ttaggcgcta attatgttat cagtggtgga
     2041 caaacaatga atccaagtac ggaagacatt gttaaagcga ttcaagaagt aaacgcagat
     2101 aaagtgattg tcttgccaaa caataagaac atcttcatgg cagctgatca agcagcagaa
     2161 gtagcagata tcccagtagc agttgttcct tcaagaacga tttcccaagg attgacagct
     2221 atgctttcat tcaatgatca agcttcttta gaagaaaaca aagaaacgat gacagaaatg
     2281 ttgtctagcg ttgtgagcgg tcaagtcact cacgcgatca gagatacctc aattgacggt
     2341 gtagagatca aagaaggaga ctttttgggc atgatcgatg gaaaaatcct gatctctaac
     2401 ccagatatca cagcaacagc gttaagtaca ctagaacaaa tgatcaatga ggacacagag
     2461 atcgtaacga tcttggcagg tgatgaagga acagttgaac aagcaaatga atttgctgag
     2521 aaactaacag aaaaacatcc tgatttggaa atcgagatcc accaaggaga tcagcctgtc
     2581 tatccttatc tattttctgc tgaataattt ctgtgtcaga atatctttct aacacagtat
     2641 tcattcataa aaggccttcg gttagtgatc aggtatgatc gctgaccaag tccttttttt
     2701 gttaaaggag ggaggaaaaa tttatgcgtt ctttagatga tccaatcact atgctggcag
     2761 gagtcggaga aaaaagagca gctagtcttg ccagcttggg catccaaacg attgaagact
     2821 tactgactta ttatcctttt cgctacgaag atattcagga aagaaatcta aacgagatcc
     2881 aggatcaaga aaaggttact ttgaaaggaa tcgtagtctc gcctcctgtg atgaatcgat
     2941 ttggttataa aaaaagccga ttgcaatttc gtatgatgca agaacatgat gtgttcaacg
     3001 tttctttttt taatcaacct tatctaaaag aaaaagtcat tttatcggag gaaatcgccg
     3061 tatacggtaa atgggatgca aagcgtaaag ccttgaacgg gatgaagatt ttaggttctc
     3121 aaacaatgga tgacttttcg ccgatatatc acgtcaacaa atcaattcgt caaacgacat
     3181 tagttgatct aatcaaaaaa gggtttcaag aatacggcac attgattgaa gaaaatctgc
     3241 caaacaacct tgtggagaaa tatcgactgc ttgaccgagc ttctgctgta agaagtatgc
     3301 attttccaaa agaccacgaa gaaaaccatc aagccaaacg acgtgtcgtt tttgaagagt
     3361 tctttttatt tcaaatgcgt cttcaaggat tgaaaaaagc agaaaaagca gaaacgaatg
     3421 gtttggagat cctttatgat gtccaaaaat tgaaacaatt tactcaaaag ctaccttttg
     3481 aactgacaaa tgcacaaaaa cgtgtaacga atgaaatctg tcgggatctt cgcagtccgc
     3541 agcatatgca gcgacttttg cagggagatg taggtagcgg aaaaacagtg gttgccgcga
     3601 ttgcgttata cgctaccgtt acagcaggtt tccaaggtgc gttgatggtg cctacagaaa
     3661 tcttggcaca acaacatatg gaaagcttgc aacagctgtt tgatcccaat gaagtaacca
     3721 tcgcattgat tacggggtca acgaaagcaa aagaacgacg tgagctcctt gaaaaactag
     3781 agcaagggga aattaacgtc gtgatcggca cacatgcatt gatccaagat ggagtcgaat
     3841 ttcagaattt aggtctagtt attacggatg agcagcaccg ttttggcgtg aaccagcgga
     3901 aagtacttcg tgaaaaaggc tggcgaccag atgtcttatt tatgacagcg actccgattc
     3961 ctagaacatt agctattaca gcatatggtg agatggatgt atctatcatt gatgaacttc
     4021 cagctggacg tatcccgatc gaaacacgct gggtaagaac acctcaattg gacagtgtac
     4081 tggattggac gaaaaaagaa ctggcaagag gacatcaaat gtatgtcatc tgtccgctga
     4141 ttgaagaatc agaagcatta gatgtaaaaa atgcagtgga aatctatgaa aaacttcgtg
     4201 aactatttgc accaaaatat gaagttggct tgttacatgg taaaatgaag aatcaggaaa
     4261 aagaagtaat catggaagcc ttcaaagaca accagatgca gatattagtt tcgactactg
     4321 taattgaagt aggggtaaac gtgccgaacg cgaccgtcat gctgatcatg gatgcggatc
     4381 gatttggatt ggcacagctg caccaacttc gtggacgtgt tggacgcgga agtgatgctt
     4441 cctactgtat cttagttgct aatcctaaaa atgaactagg tgtggaacgg atgaaaatca
     4501 tgacagagac caccaatggt tttgtactaa gcgaaaaaga tctggaatta cgtggtcctg
     4561 gtgaagtatt tggcttccgt caatctggtc tgcctcaatt tgcgattgct gacttggtga
     4621 cggatggaaa tgttttggag gtagccagag aagaggctgc agccctttgg caaataaagg
     4681 attggcagat gcttcctgag tatcagcctt tagcgcaaag cctccaaata gaaaatcagg
     4741 ataatcgtta tttcgattag cagactgtgc tatactttat actgatgacg gaaaaaggag
     4801 tggaaacaat gaaaattgcc gtagatgcaa tgggcggaga tcacgcacct caagcaattg
     4861 tagaaggcgt ggctttagcg cagcaagatt ttccagaagt agaattctta ctttacggaa
     4921 aagaagcaga aatcaagaaa tacttaacaa atgaaaaaaa catcacgatt atccatacag
     4981 acgaaaaaat caatagtgat gatgagccag taaaagcaat tcgacgaaaa aagactgcat
     5041 caatggtcct tgctgcacaa gcagtgaaaa acggtgaagc agatgctgta ttttcagcag
     5101 ggaatacagg cgcgctacta gcagcagggt tgttcatcgt aggacgtatc aaaaatatcg
     5161 aacgccctgg cttgatgtct accttgccag tagtagggaa agaaggtgcc ggtttcgata
     5221 tgctggatct tggcgctaac gcagaaaaca aaccagaaca tttgttgcaa tacggtattt
     5281 taggttcttt ttatgcacgt aaagtccgag gtatcggacg tcctcgtgtc gcactcttaa
     5341 acaacggaac agaagcaaca aaaggtagtg aagtgacgaa aaaagcctat gaactccttc
     5401 agaatgaaac aagcttgaat tttattggaa acgtagaagc tcgtgagcta ctcaatggtg
     5461 tggctgatgt agtggtcaca gatggattta caggcaacgc tgttttgaaa tctattgaag
     5521 gaacagcaat gaatatgatg aatctattaa aatctgctat tctaaatgaa gggatcaaag
     5581 gaaaaatggg cgctatgctt ttaaaagacg gcttacgttc tttgaaagca gaaatggatt
     5641 attccaaaca tggcggtgcc gttttgtttg gcttgaaagc accagtcatt aaaactcatg
     5701 gagcaacagg ccccgatgct gttcgctata cgatccgcca aatccacaca atgcttgaga
     5761 cagatgtagt tggacaatta gtcgaacaat ttgaaaaaag cgaagaatag tatttggata
     5821 tctgaagcga tccgttccat tgaaaaaaag gtggacattc ctgaaaaatg ccagtaaaat
     5881 tagccatggt gtaggtaatt gacaagtgta tgattcttgg aggtgaaaaa gttgactcgc
     5941 gaagaagtat ttaacaaagt agcgaaaatt atttcaaatc attttgagat agataccgat
     6001 aaagtgacgg atgaattgaa catcaaagat gatttaaaag ctgattcaat tagtattatg
     6061 gaattcgttt tagagcttga agatgaattc ggtacggaaa tttcagatga agacgctgaa
     6121 caaattgaaa cagtcggtgg cgcagtagat tatatcagca gccatcttaa ataatttcaa
     6181 ataaaagcaa agaaggataa aaagctgcat gcagcttttt atccttcttt ttttaatctt
     6241 tttgacacgt aaaagtcatt t
//
LOCUS       DACEMO010000149         6142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEMO010000149 DACEMO010000000
VERSION     DACEMO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.154"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(158..643)
                     /locus_tag="IW728_002174"
     CDS             complement(158..643)
                     /locus_tag="IW728_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HAQ1025517.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(645..2081)
                     /locus_tag="IW728_002175"
     CDS             complement(645..2081)
                     /locus_tag="IW728_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025518.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
     gene            complement(2114..2965)
                     /locus_tag="IW728_002176"
     CDS             complement(2114..2965)
                     /locus_tag="IW728_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025519.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(3121..3366)
                     /locus_tag="IW728_002177"
     CDS             complement(3121..3366)
                     /locus_tag="IW728_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025520.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            3471..4244
                     /locus_tag="IW728_002178"
     CDS             3471..4244
                     /locus_tag="IW728_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025521.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLNVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(4297..4611)
                     /locus_tag="IW728_002179"
     CDS             complement(4297..4611)
                     /locus_tag="IW728_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025522.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
BASE COUNT         1935 a         1082 c          769 g         2356 t
ORIGIN      
        1 cattttttat catagagttt tacttatcta tagaattatg tctgttccta attcagtttg
       61 atgaattaac tgataaaaaa atatctacta tttaaagata cagggataag taagtaaaaa
      121 aataacatgt cattagatag tagacagttc caactcttta tttacctttt ttgtatgctg
      181 cactgtcaat ccaagttcct aaattgttgt atccatcatc acttatataa tcatattggc
      241 catcaataat ttcgtcaaac cacaaatatt ctccatctgg tttttgccca attattttaa
      301 ttactgattg actagtagtt gtcatctggt taaaatcttt tattttacct atattaacgg
      361 ccaaaatagc atttcctcgc ttataactct cctggatttc atatttaaca tattctcgat
      421 caagagtttc tttacctatc agcaccacag tcactgatgt tccaattaat tgattatcta
      481 tccagttttt tatagcaata tctccattac gtttaacttt ctcaaattca gccttatcta
      541 caaaaccagc ttcctttgtt cctcgcaaag tccagctgtt tctaactacc attgctcttg
      601 ttacatctgc ataatggaaa ctaaaaaaaa cccttctcac catattaatc ctcctgaata
      661 ctatttatta aactgacaat ttgttcaact tttttatcta cttctccttc agtatctaat
      721 ttttctagcg cttgaaattt actttttaag gaatcatcaa aataatcact gaaattctct
      781 tgaatcttat caaacaaaac cttacttgca cctttggtaa atccaatcgg aactactata
      841 ttttcatttt gaacagataa ttcaaattct tcaatcattc catctgctgt aactatttca
      901 cctgattcaa gtttgtttcc aaaaacaaaa atactaatcc cagactctgc aataatgttt
      961 tctcgatatc ttctccactc atgaggcata tcaaggggaa gtggtcttaa agaaagtacg
     1021 tcttgtatgt tattttttct taaatttctt ctctgcttta gaactcctga aacaatacta
     1081 ttaccaattc caagtccgta acctgatact attctataac cggattcaga aagcctatat
     1141 gctaatttct cgacaaattt taatcctttc gattcagatg taaatggagt ataatcaaca
     1201 gcacttcccg aaataaaaat agtttttctc aaatactttt ttctaatact tttcaaaata
     1261 tcagtaatct cagaataatc atctacttca acaacgttta tcgaaaatct ggacaaatca
     1321 ttgatttgta gtcttctttt tgtcagttca taattatatc tctcttgatc aaaaaaatcc
     1381 tcagaatcga caaaattttc ctttaatatt tttctcatta tacaataatg aggtctttgg
     1441 ttttctctca aaacccatct tatatctgat aaaattcttt ctaaattagg gtctgtaaaa
     1501 ctaaatccta aaaagagaaa agtttttgtt aacaaatctc cttttaaagt ttctttaaat
     1561 aacgcgtacg aatcatcatc atatttttca taatcatctc ttgttattac tgtcgaactg
     1621 acatcatcta tatcaccatg cattttataa acaattgtat ccacatttgg attagttgtt
     1681 gctaactgag tataattttt cttaacctga gggtttttat aagcttctcg gattgatttt
     1741 tctattagat gatcataatt agttgtccaa taagtttcaa taggtaaact tgctagtatt
     1801 cgatgatttt cagatatttc taattcttca ccaaaattat ccataagtat ctgattcaaa
     1861 attcctcttg atccaccatt ttcattttta taatattgag ccagagtaac taagtctgtt
     1921 tcttttttag aatccaaccc gatttctgtt gcaggatcat taagtagttc cggccaactt
     1981 acatatcctt gtgacatcga taatcctgca cctgcaaata ttgctgcatt tcctttttca
     2041 atagcttcag agaacctctt tattaaaact ttcttatcca ttctacccgg ttcctttcga
     2101 ttcagtaaat tgattagaga ttagtgaaaa tggtgttttt atataaaata tctaatgtat
     2161 tacttccttt agtgcattcc acagcatcta aagcccattt tttcaaatca gcttttcgta
     2221 aataagttaa ttcacttaaa tttaaagaat attttatttg cagattttct tgcgaagaat
     2281 agtgctttat aatgcttcta acatcctcgg tgctcatttg aggattactt tccaatcgtg
     2341 gattcttttt ttccactatt gaagctgtcg tcagttcgct atatagccat ggcgaatacg
     2401 taaatttctg ttcattagtg atttcattgc tcaaactaat tgaattaggt gtattaagaa
     2461 aaaacagaca ttctgtacta tcaatcatat tatttaaagc tgtagaaagc atcatatgaa
     2521 catgactagt tgttacattt cttttatcat aatcatatgt ttttgtttgt tccttatagc
     2581 aatatttttg gtctattttt tttaataaat cagaactgta tccccaaacc gaagaatcaa
     2641 taaatgcagt aagaccaaaa ttttcaaata gccatgatgc aaaacgtata gctaattttt
     2701 catcagcatg agagtgagat ataaaaatat ctgcttttat agttggaaac caatctttaa
     2761 taatttcgtc tgcatctaga attttgctgt ccttagaata ataatcaact aaatcatttt
     2821 cactttcaaa tagattttct attctcttgt taatagattt ttggttgtga tctatttgat
     2881 ttactacatg tgtatatgat tgcgagcttg aggagaatgc atttaagtca gcatttgtaa
     2941 aatccaattc atatgcctta tacaaggata tccctccatt cttgtatttt gatccacaat
     3001 ttattaggca acataacagt ataccctatc gttacatatc ccattcatta ttataccagt
     3061 atttcttcgt gacacataag ggtgtaacat aataatttca aacgaaatga ctacaagtag
     3121 tcattgaagc aaatcttcat aagacacctg tagaatatct ttgatggctt tcaattgatc
     3181 caacttaatg tgttggcggc caccttcaat cttaaccaac gtttccctag ttatagcaat
     3241 tttacgaagc tgtaggtctc taatcagctc ggtttgacca attccttttt ctaatcggac
     3301 tcgtctaata ttgctaccga ttacattttc tttatcaata attttttcct ctttcaagaa
     3361 tagcacacct tttggactaa aattagtcct ttttcttatt aatcttaaag attcggtatg
     3421 ctacaatggg actagtatta gtccatattc aattttaagg aggaattttt ttgaagaaat
     3481 ttgcaatcat tctaacttgc gctttactca tcggaactat aagcgggtgt accacttctg
     3541 acacaagttc tacaagtacg gaaactagta caacaacctc agaaaagaat tacccaatac
     3601 atttttttcc ggttccagat ggatttgaag cttattatca tgataaaaaa ctaaatgttc
     3661 ctttatcaga aactgaatct actgaaaaga ttttgccaat tgaaattgaa agaaatcaag
     3721 acgatataaa actaaaagct gatcttccct tactgaacag cacacaaatt gatagacatg
     3781 aaaatattaa agttaatatt gaaaaaggag cgttagcaca actttatact gaaaaagttg
     3841 aacaattttt tgaggctggt gccaataaag acttttcaaa aataagcgat tattcggata
     3901 acttttttaa tgagacccaa gatgaaatca cgaactcaac ttttatggct gaagatatgt
     3961 acaatatgaa gttgaaaaaa ataaagtcgt atgattatcc attaaatcta acattaaata
     4021 aagggatact ctcttttgta ttagcaggtt acgttgacta tgaatataca gcagattatt
     4081 tggagacgcc ccttcaacac gataataacg atattaattt tagatttatc tacaattcaa
     4141 aaagtaaaac atggttagtt gatcaattga gcactataaa tacgggattt catgaattac
     4201 ctaagcctga caatgaaact gctttggtca ttaaaacatt ctaaaaaaga aacgttgaag
     4261 gaataattat tctttcaacg tttcttttat tgtttttcag tttacggtct tacctgagcg
     4321 aataatctga tttctaaatg attggaaaac ttcttgtggt tgataataat ctgtatctat
     4381 ttcaaatgtt tctgagaata attcattgaa tttttctact gaaatgatga ttttaccatt
     4441 ctcctctcga ctgttttcta ttatttctgc ctctaattca aataaatcta gtgacgaact
     4501 aaaatcataa atcctgtcat attgaacaaa aaaatcaaga aaaagttgct caaactcttc
     4561 cttatttatc actagtgttt caagcggaac aactttattt tcatcagcca ttcttccaat
     4621 ttcctcactt tcattttcaa ttgtccattg ttgcggacgt atcagatttt ctctatcatt
     4681 tttcagtgta tagatcactt gtaatacaca acaatgcttt gtaaacttat ttttttagtt
     4741 agtcgaatct ttttaaatgt gcgtttcata ccatgcgctt gatatatttt ctcctatttt
     4801 ttaaatcgtt tatcacaact tttatcgttt tttcttctga aaaaaatctt gtttacatcg
     4861 ttacatagtt tccaaaaaga tacattacta gtagaaacaa caagattaat tttacttccc
     4921 actgaaatgg gaagtatatt ttaaatattt tttctccttc aacttacact ataaagtatc
     4981 ttaatcacac gatatgctag ccactaaatt catcatattt gattcctttt ttaagataat
     5041 gtagcatctt tttatccaat aacgcaattg tttttaagga taaatttctt aagatggtac
     5101 atttcaaaat ggcatttgac atatgaaaca aaaaagaaag aaaagagcat aagggaaaac
     5161 tgatagcacc ttcctacagg aaggtggcta agttggctgt gccaactggt tttctttcaa
     5221 aatcatttca taatttttga tatcataaaa aaatccattt tggacctatt ttcggtcata
     5281 atttagtacc tacttttggt catagtttcg tccgtagttt tggtcatagt gactaaggct
     5341 tttctgttca caaaattggt ttaaatttgc tttctttaac acttcattta tatcatagca
     5401 attataatgt ttacacaaca tcattcctaa ttttaacggt tgttcctctt tatatttttt
     5461 aagttctaca tttttttgcg tttaattact tttctctttt ctttatattc cgctccaact
     5521 ttctctttaa tagtgataat agaattacat atttttgtta aacttcctta aaaaaattgg
     5581 atctcgatta ctcaaatttg cttgcaggct tttctaattg ctttgttttt tcttcctaat
     5641 tatttgattt tatttccatt ttcttctagt gaaaatttat tatttttaat aatcgtacgc
     5701 gtagaactga tttcattttt tgtatctgtt tgattatcta acgtttgaaa ttcaaagttt
     5761 tgattggctt tatcaatatc tttttatact actttatttg caagatatat tttcttgctt
     5821 aatttggtca tagtttcatt cgtcacttcg gtgatatttt tttaatttgc aaataaaaag
     5881 agttaaaccg atatacatta ggttcaactc ttttgcaggt ttcatgggtg tactatcact
     5941 tttaatctga tagatgtaaa cctctcttta tctttttgtt acttcaattt tcaacaatag
     6001 ctcctttata ggttgtcaat attgctctac gtattctggc tttttaaccc attatttctc
     6061 taaagtggaa gagaataatc gcttaatccc cactaattcc ttgtcgtaaa ttcatgtcaa
     6121 atacaatttt taaaagccat tt
//
LOCUS       DACEMO010000150         6127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEMO010000150 DACEMO010000000
VERSION     DACEMO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.155"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..226
                     /locus_tag="IW728_002180"
                     /pseudo
     CDS             1..226
                     /locus_tag="IW728_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            352..1323
                     /locus_tag="IW728_002181"
     CDS             352..1323
                     /locus_tag="IW728_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1025523.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            1463..2776
                     /locus_tag="IW728_002182"
     CDS             1463..2776
                     /locus_tag="IW728_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1025524.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            2819..3685
                     /locus_tag="IW728_002183"
     CDS             2819..3685
                     /locus_tag="IW728_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1025525.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            3682..4530
                     /locus_tag="IW728_002184"
     CDS             3682..4530
                     /locus_tag="IW728_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1025526.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            4544..6016
                     /locus_tag="IW728_002185"
     CDS             4544..6016
                     /locus_tag="IW728_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ1025527.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6033..>6127
                     /locus_tag="IW728_002186"
     CDS             6033..>6127
                     /locus_tag="IW728_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAQ1025528.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPT"
BASE COUNT         1913 a         1038 c         1335 g         1841 t
ORIGIN      
        1 tcttgaaaaa ttaattaaag acttagataa agccattgac gacttagtcg tggtgctacc
       61 ggagtatcaa tgtttaacca gtatcccagg tattggtcct gtttaggctg ctggaatact
      121 cgctgaaatt ggtccaattg aacgtttttc tgatgaaata aaattgacaa agtatgccgg
      181 cctatattgg cgacagagtc aatccggtaa atctgaatac gaaaattttt ttacgaaaag
      241 cttcacgtct aatatctatt ttaaaataaa aatattcctg ctaatttgga atcgtttcct
      301 tcttttggta taataatacc tgaaattctt ttgagaggag ttgttcgtga agtgaaaact
      361 actatgaagg atgttgctgc gttagcagga gttggtgtgg gtacggtatc tagagtaata
      421 aatggagtaa aggtgaaaga ggttaccaaa gaaaaagtaa tggctgcaat tagtgagctt
      481 aattatgagc ctgatgaata tgccagaggt atgaaaacga atagtagcaa tacaatagct
      541 cttattttac ctacgatttg gcatcccttc ttttccgagt ttgcctacta tgtagaggaa
      601 gctctcagcc aaaaaaatta taagcttttt ttgtgtaatg cgaacggcag tgcggaaaaa
      661 gaaaaagaat atatccaaat ggtcaagcag agtaaagtag acggaattat aggtattacc
      721 tattcagaca ttgataaata tgtatcatca aatttaccat ttgtcagtat agaccgccat
      781 ttttcggaac aggtttctta tgtaacggca gataattatt taggtgggca aattgccgct
      841 caagaactac ttactcgtaa ctgtaaaaac ttgtgctatg ttgggggtgt ttcaccttac
      901 cctaacgaaa caaacaatcg taaagctggt ttttacaata tttgtaagga aaataaaata
      961 gctgtgaagg tattggatat gcctgaacca attagcaatt tagaggaaca attgctcgct
     1021 tttttttcgg ataatcctga aattgatagc atttttacca tcaatgattt catggctttg
     1081 gatgtgataa aagcccaaaa aaaactaggg aaggaagcac ctaaggattt tcaaattatt
     1141 ggttttgatg gagtcaagct atcgttgcaa aaagactatt tactatcaac gattgtacag
     1201 ccgatagagg aaatggccaa ggtttcagta gaaacactca tggcgatgat tttaaatgaa
     1261 gaaccgccta agcgtgtggt agtaccggtt ttctttcacg ctggagagac gactaaagag
     1321 taaaaaaccg gtttataacg atttttttga gataggttga cagaaagtga tttcaatgat
     1381 atcttaaaac caattaattg gaaacgttac caattaattg aaaaataaaa cggaaacgtt
     1441 tcaaaaaata ggaggaacat cgatgaaaaa gagaatgtta gcaattgcag caattttagc
     1501 attaacagct ggaggacttg caggatgtgg cagtaaggag aaaagttcag gtagcagtga
     1561 tccaggagaa attaagattt gggttcagtt ttctgatgaa acgcctgaag gaaaagcttg
     1621 ggcagagatt gtgaacaatt ttaatgatga atacgagggt gaatataaag ctgttactga
     1681 gtatattcct cgtagcggaa gcggcggtgg atatgaggat aaagtaaatg ctgcaattac
     1741 aaccaatacc cttcctgatg ttattacgtt agatggaccg aatactgcag cttatgctca
     1801 ttcaggagtc attgctccat tagatgatta tttgaaagat gcggacatgg atgatgtatt
     1861 agacagcatt attcagcaag gaacctacga tggcaaaatg tatgcgtttg gtttctcaga
     1921 gtccagtgtg ggtgtctatt acaataagtc gctgttcaaa gatgctggaa ttacagaaga
     1981 tcaattgccg acgttagaag atccttggac ttggacggaa tttcaagcaa tttgccaaaa
     2041 gctgactgat aaatacaaaa cgcctgcaat tgatatgcaa atggcgtcgg gagacgaaat
     2101 gctgacctac gcatatacac catttatatg gtcgaatggc ggcgatgtcg ttaatgctga
     2161 tggaaccaaa gccgaagact attttaacgg taaaaaatcc gttgaagcgt tggaatttat
     2221 tcaagaattg gttgaaaaag gttacaccac aatcagtcct attgaaaaag gatttgaaac
     2281 tggcaaatat gccatgatgc tgagcggttc ttggacaatg gcagatttag aggaaaacta
     2341 tcaagagatt gattatggta ttttgcctta ccccgtttca gatgcaactg gggagttagt
     2401 ttcaccaacc ggaagctggc agttagcaat gactgagaaa acagagaaaa aggatgcttc
     2461 tgcggccttt attaagtttg caaccagtac agaatctagt cgtattatga gtttagggaa
     2521 cagtgttcta cctatcagaa attcaacagt tgatttaatt aaagatgaag tgtcggaagg
     2581 aatgcaggta ttaattaagc aaaaccaaat ttctggacat gcccgcccag tggttgtagc
     2641 ttatccgcaa gtatctagag cattccaaca agcagttcaa gatattagct acttcgagga
     2701 aaacccagat gtacaaaaag tagcagatag tcgcgctgct gaaatgcaac aggcaattga
     2761 tacttcacta aaatagaagt ttctaaaggg ggccaatcaa gtgttaaaaa aaccgacaat
     2821 gaaagacaat ttgataggtt acggctttct agcgccagct ttgctgctgc tgacgatttt
     2881 tctgatcata cccgtaatca tggtaattta ttatgccttt actgattatt atttgttaat
     2941 gccagatgct agacaattta tcggcttaga aaattttaag cgactgttcg ctgatccaat
     3001 ttttgtaaaa agtatttgga acacggctaa atttgttgtt tggattattc cggtccaaat
     3061 aggggcagct ttaggaatgg cacttattat caacaaaaat cgtaaaggaa atatcttttt
     3121 taaagtagcc ttttttgcgc cagtcgtgat gtccttagtc gttatttcga ttttatggtt
     3181 gtacttgtta aatcctaatg aaggtctggt taatacgcta tttgcaaaga ttggaattgc
     3241 ttcgcagcct tttttgacta gtcccaaaca agcaatgtat acgcttgtgt tcgtatcggc
     3301 atggcaaggt gcaggatatc agatgctgct gtttttaggg ggaatgcaaa atattccgca
     3361 agacgtttat gaggcagccg aaattgatgg tttttctaag tttcagcaat ttcgctatat
     3421 caccatgcca ttattaaagc ccacggcgat ttttatttta cttaccacat tgatttcggc
     3481 ctttaaacta atcattcagc caatggtaat gactcaaggt ggaccaatga attctactat
     3541 gacaatggtt tattacattt accaaactgg atttacagac cgtatggtag gctactccag
     3601 ctccattgcg ttaatcttta ccactatgat tggtttaatt acaattgctc aaagaaaatt
     3661 ggtaaaggag gatgatccag catgaccaag aaatttggac cgctaatgat cgtagaatat
     3721 tgtttgctgt tccttttagc ggcattattc attttcccga tgctgtggat ggtagtttcc
     3781 tctatgaagc ctgaagcaga tgtctatcaa aatttagctt ccttcaaagc ttttttacct
     3841 agtttgaatc cagctaattg gtttaaaact tatcaagagg ttatttcccg gttcagtgtg
     3901 ggaacttatt tattaaacag tattttttat ggtttaacct ttgcctgtgg gtcaatcatt
     3961 gtaaactctt tggcgggttt tgcctttgcg aaggttaatt tttcaggcaa gaaaattatc
     4021 tttggagctt tgttagctct gctgattgtc ccgatggaga cggttttaat tccgcaattt
     4081 accatcgtta attcactagg cttagtgaat actcgcttgg cagtgatttt accagcgatg
     4141 gccagcgcgt ttaatatcta tctgtttaga aatttcttta tcgcgattcc agaagaaatc
     4201 attgaatcag cgaaattaga tggtgcaaat attgttaaaa ttttctttca catcatgctg
     4261 ccaatgtcta aaccagccat agccacagta ggtgtcttat cctttatctc cagctggaat
     4321 gattatattt ggccgttaat ggtcttaact gacagcagca aattttcaat gcaagtggca
     4381 attactacga tcaatactac acagccagtt tatatcaatc aagtgatggc tgttttaacc
     4441 attgcaacga ttccactgat tattgtttat atcgttgcac aaaaatatat tctgcaggga
     4501 cttggcggtt ctggaacggg tataaaatag gagtgaatag tgaatgttgc aaaaactttt
     4561 aaaggccaat gagtttattg agaaaaatgc tcaaacagtc aatcaagaat accggaataa
     4621 atttcatttg atggctccga ttggctggat gaatgatcca aatggttttg tttattatca
     4681 agaggagtat cacttgtttt atcaatatta tccttacgac agcatctggg gaccaatgca
     4741 ttggggccat gctaaaagca aggatctaat tcactgggag catttgccag tagcactggc
     4801 accaggtgaa agctatgaca aggaaggctg tttttccggc agtgccatag aaaaggatgg
     4861 caagctgtat ttgatttata caggacacaa tgttgtggaa gggcaagttc gacaagttca
     4921 gtgtttggca gtttcagaag atggcattca ctttgaaaaa tatgccggaa atccaatcat
     4981 cgcagaagat cacttagctt ctgttgcaac gaccgaagat ttccgcgatc ccaaagtgtt
     5041 tcagcgaaaa gaagactatt ttactgtagt cgctactaaa actgcagatg ctcgtggaca
     5101 aattgtgatg ttccattcca aagatttact aacttgggag ttttattccg tattgctgga
     5161 gggaactcca gaacaaggca ttgtatggga atgcccagac ttgtttgaac tagatggcaa
     5221 agaagtcttg attctttctc ctattcaaat gactccacaa ggcaatgctt accaaaatac
     5281 gaactcaact gttgctttca tcggtactgt cgattggcaa acaggcaaat ttctagtaga
     5341 gaattttcat gaaatcgatg gcggtttaga tttttatgca ccccagacct gtgaaaacga
     5401 agcaggccaa agaataatga ttgcttggat gcaaatgtgg cagcgtaaaa tacctacgaa
     5461 tgatctgggc catggttggt caggttccat gaccttgcct agagagctga gggtggctga
     5521 taatcaattg ttgcaacggc cagtttcatc aatttatcaa gtaattgatc cgcaggaaag
     5581 gattgaatgg gcgaagactg aagagaatcg cttcttactg aaggacttag tatatgacca
     5641 gcactattta cgcttggaaa tcaatttaaa acaggcaacg aaactagaac ttcactatgc
     5701 gaagggaccc gaccatacat tactgcttaa ctatgatgtt gaacgggagt gctttactgc
     5761 ttccagggaa cacatgccgt atgatttatc aggagctgaa aaggaagtgt tgaaaaagcg
     5821 cagtgtgacg attcctttgg aaaataattg tctaaccatc gaaattttcc gtgatacttc
     5881 agctattgag ctttttttaa atgatcgtct gactatgagt acaacttttt atgagacaga
     5941 aaaaggacag gattttcttt tgctggcaga cagtgaagta accttttcat tggaaagcgg
     6001 cttaattaat ctatagaagg gagagctcag tgatgattta tggttcaatc gaagcaggcg
     6061 gcacaaaatt tgtgtgtgcc attggcgatg aagaaatgac gatcaaggaa agagtgagct
     6121 ttccgac
//
LOCUS       DACEMO010000151         6110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEMO010000151 DACEMO010000000
VERSION     DACEMO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.156"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(99..1217)
                     /locus_tag="IW728_002187"
     CDS             complement(99..1217)
                     /locus_tag="IW728_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025529.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1688..2995
                     /locus_tag="IW728_002188"
     CDS             1688..2995
                     /locus_tag="IW728_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1025530.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            3114..4313
                     /locus_tag="IW728_002189"
     CDS             3114..4313
                     /locus_tag="IW728_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1025531.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4340..5608)
                     /locus_tag="IW728_002190"
     CDS             complement(4340..5608)
                     /locus_tag="IW728_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1025532.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(5771..>6110)
                     /locus_tag="IW728_002191"
     CDS             complement(5771..>6110)
                     /locus_tag="IW728_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
                     /protein_id="HAQ1025533.1"
                     /translation="LKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTL
                     SPTINKGICAICQKTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFY
                     EFAERVKKER"
BASE COUNT         1860 a         1296 c         1090 g         1864 t
ORIGIN      
        1 taatttttgt caatctcttc ttatttcttg aagctgacca cttctgtcac aacctcttcc
       61 ttaatactca ggtcaaaaca ctgatttcac aacctctttt aataacgaag aatcgctgtc
      121 agactctttt catctggcgc atctttttga tccaaaataa acactttccc gccattaatg
      181 atcacatcat ctgctaatgc attcaacact tgacgccgat catattctgt ttcaggatct
      241 tcaccgaaac catcagctaa gtttgacgta gcgataaata aatgtgacac ttttccttct
      301 ttcgcagctg gaacgatatc aactaactga tcgatgaatt tcttatctaa gagtttattg
      361 tattcttttg attcttgagc tgcgagttca cttgacagct tcttcgcccc ttcttcgata
      421 tctctaaagg atagctgcgc aggagaagca gaaatcgcaa cgtctgcaca ataataggga
      481 tttttagcaa tttttttgaa catcgtctga ttttctggta atgcaaataa ataaatcggc
      541 cattgttcag gattttcgaa agaatccttt aagtacgtat caacagcttg gtaatagttg
      601 acccaatcga tctctacttc ttcatctttt ggatttacgc catggaaagt tactccatct
      661 cgggctccgc ctcctccatt agaagagaag ttcaaaccgc cacctgtcag ttcagatccc
      721 aatgcagtaa cgacatcaac cggtgcccct tctggtagag atacttcttt tacttgtttg
      781 ttgctgacct catacaattt catggagtcg cggttcaatc ccaataaata ataagaatag
      841 ttgaactgcc catttttgat aatagccagt aaataaggaa gatcacttac atagtattga
      901 tcgtctaccg ggatactcaa acgatggata taggtctggt cagcagttat aatgaccgct
      961 acactcgtag ttgcactacg ccaaaacgaa ggatctccta acaaattttc aattttttct
     1021 tggaacggag accagtcatg ttcggaatat tttttctcaa aacgtttctt tgcttctttc
     1081 gcaaaatttt tgaatttgat ctgatctttt tccacatctt gatgggcgac atgcgtattc
     1141 agcatgatcg tgacgaatgg tcctttagcg ccttctgaag tcaattgtgt caataattct
     1201 ttatcctttt ttgccataaa cgtacccctc ctaaaaaaat cattcctttg tttctatcta
     1261 aagtctacca caacactatt tttccataca ataaaacgta tctaaaaaaa taaaacggtt
     1321 tctacggttt taaatttagc tattttttta agttatcttc ataatttcct caattattta
     1381 gccccagtct tcaactattc ttcatgaaat gctgataaac taagaaaaag aataaaagag
     1441 gtggatctta tgcatttaat tatgattttc agtttattaa gtattatcag tttatttgct
     1501 ttcagcgctt cccgcttatt agatgcggaa acacggccta ttcctgtaaa agtacgtaaa
     1561 aacgaccgct aaacttccat aacgcgcgtt cttatcctaa agttttttca gaaacattga
     1621 aactctctcc cctctctgat aaaattttaa tttgtagcga aacaaattaa ttacaaggag
     1681 atcggatatg tccataaaaa aaacacgtct agtcctatta ttggctagtc tcattctttg
     1741 ccttgcttct tttctaccag ctgttgctgt gcaagctgaa gatacgttca aagtaaatgc
     1801 aaaagcagct tttgctgtag atgcgcaaag cggtaagatt ctttatgatc aagatggaga
     1861 aaaaaccatg gggatcgctt caatcacaaa aatcatcggt ctttacatcg tccttgatca
     1921 agtaaaagaa ggaaaactgt catgggatga taaagtttcg atttcagatt atgcagaaaa
     1981 tttgagtatc accccagatt tatcgaatgt tcctttgcat aaagaaaaca cctacactgt
     2041 caaagaatta ttcgactctg caatcatcca gtcagccaat gcttcaatgg tggccttggc
     2101 agaaaaaatc tctggcagcg aagctaaatt cactgaacga atgaaagaac aattgaaaga
     2161 ttggggaatc aaagatgcaa caatcgttaa tgcatcagga ttgaacaact cttatcttgg
     2221 cgaaaaccga ccagaaggca ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt
     2281 ggccatcgtt gcccgccatt tgatcttgga tttcccagaa atactggatg tatcaagtac
     2341 tacgacccaa atgtttggcg aaaatacgca atcaccagtg gaaatggtca actggaactg
     2401 gatgctccca ggattcgtta attacaagga aggcgtggac ggattaaaaa ctggtacgac
     2461 tgagcttgca ggtgcttgtt tcgttggtac gatcactaaa aacgatcaac ggatcatcac
     2521 tgtcgtattg aatgcggacg gccatgcgga aaacccgagt gcccgcttca atgaaacaga
     2581 ccgtttgatg gactactgct atgacaattg gtcagaaaaa gagctcggaa aagctaacgc
     2641 aagtattcct agtctaaaaa ccattgatgt aaaagacgga aaagaatcaa cagttcctgt
     2701 tgtgcttaaa agccctgtaa aagtatgggt acgaaacgat atggatactg gcaaattaac
     2761 gatcacgcca acacttgaca agaagcaagt cactgacaat gagatccaag cacctatgga
     2821 taaaggtaca aaaatcggta ccgctacgat caccttggca gacgataaat taggctattt
     2881 agaagacaac aatgcaccat caacagagat cattacggat aaaacagtgg aaaaagcgaa
     2941 tatattcgtg attggctggc gacgcgtcgc agaatttttc gacaacttgt tttaattgaa
     3001 caataagaaa aacatctttc catcggaaag atgttttttt gctttttcta gtttaaataa
     3061 cagctttttt catctatttt cgttatactg tataagaaaa aggagttgaa tttatgaatc
     3121 taaccagaga tctgatcttt ttagttctct atcttattgg ggtattctct atcgtttatt
     3181 ggcgaaaaca gaagaaggat ccttccttgt tcttttacgg cagtacgtgg acatttacgg
     3241 taatcttaac ttattttgtc gtgttggaaa ttttttattc gaccttttat tttttgattc
     3301 cattgttgtt ctttagtatc tttgcttttt cttatttcac agaaaaacga aggctgctga
     3361 atggtctttt attcaatgtt ttcctcatca gtttcggcat ctacttgttt gtcttgctgt
     3421 atgaaacgca aaatattttc cttggcggac tgatcgcctt gatcacgatt ccattgctac
     3481 ttgtcctttt atttggtatt tatggattga ttgtttttct tttttggaac ggggtcacag
     3541 ttttacgtag agaatcgcat tcattagcta atcttttaac gttgatacta gccatttttc
     3601 ttacactgtt tcttgttttt gactttttct tgttaaaata tttgccgcaa tggataaatg
     3661 ctttgttttt ctgcgttccc ctgatcttga tctatctatt tatcgtattc tacaactttt
     3721 tgactgtctc attcttgtat cagttcaacc ggcctcgata caatcaagac ttcatcgttg
     3781 tgttaggcgc tgggttgatc aatggagaaa cagtttctcc tcttctagcc aagcgaatca
     3841 ataaggcaat tgctttttat cgggctcaaa gccgtgctac attaaatccg cccatcttat
     3901 tgatgtctgg cgggcaagga gcagacgaaa aagtaccaga ggccattgcg atgaaacaat
     3961 atgccatgga acaaggaatc cctgaaagag acattcttgt cgaaaccaat tcgacaacca
     4021 ctttggagaa catgctctat tcaaaggaaa tcatggatca gcaaatgaaa gggccatacc
     4081 gcgcgatctt tagttccaac aattaccaca tttttagagc cggtctttat gcgtggcaag
     4141 ccaaattgaa agcaaatggt attggtgcaa aaacagcttt ctactacttg cctaatgcgt
     4201 ttttaagaga atacatcgcc atcttgatga tgaacaaaaa aagacatatg atcatttgcg
     4261 gattgctttt cgcaggctcc gcattcttat cgctgattac tttacttttc taaaaaaaca
     4321 aggtgtgaca aaacatatgt cacaccttgt ttttttcttt cattctctta actattggca
     4381 aaaagagtat cccttttttg accgctactt gctgggcaga ataaataccg ctgttgcctg
     4441 aacacataaa gctgatcaag cagatcataa agaagtatac tgccgcttgt gaaccgaaca
     4501 attcgatccc catcacaaag caggcaatcg gtgtatttgt cgcaccagaa aatactccga
     4561 taaagcccaa tcctgcaaga aaaggaacag aaacatgaag caaaggcgct agtgctgcac
     4621 caagcgttgc accaatttca aacaatggtg tcacttctcc cccttgatat cccgcgccta
     4681 aagacaaaac tgtgaagaat aacttcccaa taaaatcata gacatgtgcc tgtccattaa
     4741 atgattcttg aagtaacgga agactcaatc cgagataacg ttgtgtttgc aaaatcaatg
     4801 ctgctgccac aactacagct ccacctatcg tatttcgcca aacaggatga ggaatccatt
     4861 gtgcatatcg cgcttttaaa aagacgatcg aacggctgaa tacccaacca atcaaaccaa
     4921 aagcaatccc agcaacaaaa actttgaaga acagcaacat actccattca ggaatcacac
     4981 ccatttggta atgtagatga gaaacaccat agctttctgt catgaaattt gcaaacagtg
     5041 cagcaaagaa gctagggaag atcgcttctg ttcggacttt cccgattgcc aacacttcta
     5101 atccaaataa tgttccagct aaaggtgtgc caaatacaga actaaaaccc gcactgattc
     5161 cactgataat caagatttcc cgttctgctt tatccaatcg gaacaaacgt gctgtattat
     5221 ctgctaacga tccccccatt tggactgctg ttccttcacg gccaacagac cccccaaata
     5281 aatgggtagt tatggttcca aacagtgtaa gtgtaataag ccgcaacggg attttttctt
     5341 ctccaccatt tccttgatcg atcactaagt tgtttccacg actagaaaga ccgccgtgat
     5401 acgcatagag ataagcaaaa agcgcaccac taactggtag tagataaagg agccaaggat
     5461 gtgatagtcg gatatctgtc acaactgtca ggctatttaa gaaaaaagca gacaagctcc
     5521 ccatgaatat accgagaatc gctgtgatca ccagccactt cagcatgtac actaaaatgc
     5581 gcattggact cggtaattgt ttttccataa aaaattcctc ctcaataaaa aaacgcctac
     5641 taaagaaagg ggctgtcccc agactttagt aggcgtcatt agctatcttt cgttagcatt
     5701 taaaggcgaa caccatcacc tattcacttc cattaaaaga tagcatattt ttctgtaaaa
     5761 aacaataaaa ttatcgttct ttctttacac gttcagcaaa ttcatagaaa ggagccaatt
     5821 tcgttaactg ttgattacat tggtcactat catgacaaat ataattcccc tttttcgtat
     5881 acgtgccatc agaacctgct ttcgttgtag aaagaaaaag cgacacattc gatgttttct
     5941 gacagattgc acagattccc ttatttatcg ttggtgataa agtaccgcta atccctttta
     6001 atttttcatc ttcgtaatag accatgaatt ttttctgcgt acctggatca ttccagccga
     6061 tgtacgtatg ttcttgcaag tcgatttcat tccaagctgg ttgtttgagt
//
LOCUS       DACEMO010000152         6011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEMO010000152 DACEMO010000000
VERSION     DACEMO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.157"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(437..610)
                     /locus_tag="IW728_002192"
     CDS             complement(437..610)
                     /locus_tag="IW728_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAQ1025534.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            complement(953..1642)
                     /locus_tag="IW728_002193"
     CDS             complement(953..1642)
                     /locus_tag="IW728_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1025535.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYIGHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1705..2469)
                     /locus_tag="IW728_002194"
     CDS             complement(1705..2469)
                     /locus_tag="IW728_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1025536.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIVKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(2497..3195)
                     /locus_tag="IW728_002195"
     CDS             complement(2497..3195)
                     /locus_tag="IW728_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1025537.1"
                     /translation="MARTQKQKAAQTHVVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTIAANIAVVFAKSGQRVLLVDADLRKPVVYETFQLNNAS
                     GLSTALSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYRN"
     gene            complement(3207..3986)
                     /locus_tag="IW728_002196"
     CDS             complement(3207..3986)
                     /locus_tag="IW728_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1025538.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4002..4946)
                     /locus_tag="IW728_002197"
     CDS             complement(4002..4946)
                     /locus_tag="IW728_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1025539.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4992..5771)
                     /locus_tag="IW728_002198"
     CDS             complement(4992..5771)
                     /locus_tag="IW728_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025540.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKERVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
BASE COUNT         1640 a         1229 c          985 g         2157 t
ORIGIN      
        1 atcttaatta tctatccatt tcatcctacg cattcttgcg taaatacaat tcactctgtt
       61 gagctgatat aaatcattgg tggtacaaag ataaaatgat aactgtttct atacctccaa
      121 aaattccagt aaatatatct atagatagtt tcaaaaaaaa ttgtcttagt ttcataatat
      181 ttaagatagt ggtacatact ttataacaac gtacttttcc aaatattatt ttccgttatg
      241 aacatccaat atattacaaa gtttaaaagt ttagatgaac tataatcttc atttctgcac
      301 agtggataat aaattcttat agataaaaaa tagcctcttc taaagtcaca aataccttac
      361 tcccacattt taagtaaaca ctctgcagca ttctctttgt tataaaatga tagatattcc
      421 tcaattttat ctcttattat gttattatca gttgatacca ttccattaat gtttcagaat
      481 aattacaata ttttgggacc ggataactcc cttttgctcg taaccactca tatccgttag
      541 gattcactaa aagtttaccg tttatttttc gattcttttt ttgaaccgtc ccatgaacat
      601 accgattcaa caagctaaat tataaaaatt ggtgtgctgt cattattttt tggaaaattt
      661 attaatggct atcaatatac ttttgtatca tactaaatag ctcgtcagtt cctatatcgg
      721 atataccaat attaaagcat ttagatcatt atatttaaga gtggtatcat cccaaaattt
      781 cgaatatttt cttttatata agaataatga tactgaattg ccccattcac ctttcttttt
      841 ttagattttc tacaagtgat tcaaaccctc cataattaca gttattactt ttgaaacgat
      901 aatatgaaca tgtgtaatct tttttattaa caatccatca ttctatccct aactaatatg
      961 ctccattggg cttgacaata acataaaacg tttttaaaat caacctcaag tcgtacatta
     1021 aacttcgatg accgatatac tctaaatcca actcaaccat tttatggaaa ctaacattat
     1081 ttctatcact aacttgccac aaacctgaac aaccaggttt tacatacaag cgttgaagat
     1141 catatctcgt gtactcagct acttccctct ccaaaggtgg ccgtggacca attagactca
     1201 tatcgccttt taaaacattc catagctgtg gcaattcatc aatgcttttg gcacggataa
     1261 ttttacctat tttagtgaca cgcggatctt ctttcatctt gaacattgca ccttctattt
     1321 cattttgatc aagtaattct tccaattttt cttcagcatc taaacacatc gaacggaatt
     1381 tatgcatttt gaatgttttt ccatttttac caacacgtgt atggctaaaa aatattggac
     1441 ttcctggctc ttcttttctg attcgataag ctacatagaa catcaaagga gaagctaaaa
     1501 tcaaaccaat aatgcttcct acaatatcaa tcattctttt aaaaaataaa tagatatatc
     1561 gtttttcaac tttttcttta tccaaccaaa cttgattagg atgtacttca ctaatacgag
     1621 ataataaatc ttcactttcc atactttcag tccctttcac tatcttcact ctctcctcat
     1681 ttgtttttat ctacgaataa agaactaaaa taaaccaaac ttcttctttt ttacctcttc
     1741 atagattgga taactgatct catccccgtt gatcagatcc tttgcgactt gtttcatctg
     1801 tactactttt tccttaccaa agtccttgac aatctgctca tagcactctt ttaagtaaaa
     1861 ggtgcgctta gagaccccgt gtgcatcaga agcagccatc tgcacgagat tatgtttgac
     1921 cattgttttg gctgttttct ggatgtcttt gccaaataca ccaacataac ttggcgcagt
     1981 aatttgggcc aaacatccca tatctaggaa cggaagcaaa tgatttgggt cttctcgaaa
     2041 atgcgcgttg cgttctggat ggacgatcac tggtgtcttt cctagttcta acaactcaaa
     2101 aaaaagccat tctgcatata acggcacttc cagtgtcgga aattcgatca gaagatatgt
     2161 atcatctaaa tctgtaaata aaatttcatc tcggtggatg tcttcgatga gcgttccggt
     2221 aatacgtact tcttgtcctt ccaataacgt caaaggcaac tgtcttttct ctaattcggc
     2281 ttgtaacgaa gcgactagag aaatgacttg tgatttttca ttgctgtatt ttccattatt
     2341 gtggtgtggt gtgcataaaa tatgcgtgat gccttgctga atcgcttttt cagccattgc
     2401 aatacttgct tctaagtttt ctgccccgtc atcgattcct ggtaggatat ggcaatgtag
     2461 atcgatcatt tctcttgtct cctctcttct tattccttaa tttctgtagt aataaccagc
     2521 atctttgcta tgttctgctc cgttgtatac aacacctaag actcgtgctt gtaccatctc
     2581 tagcagttct tttgctttag tcaaggattc tttacgtgat gtattttcac gtacgaccaa
     2641 taaagtgcca tccgtttttg aagacatgat ctgtgcatct gttacggcta ctactggtgg
     2701 catgtcgaag atcactacat caaatagttg tcttgcttct gctaaaatct gatccattct
     2761 tggcgagctt aataactctg atggatttgg tggtttaggt ccacttggca gaatcgacaa
     2821 attctcgact ggtgttcgtt ggatctcatc tgctacactt cctgatgaac taagtgctgt
     2881 acttaatcca ctggcattgt tcagctggaa tgtctcatag acgacgggtt tacgtaaatc
     2941 tgcatcgact agtagaactc gttgaccgga tttagcaaaa acgactgcaa tgtttgctgc
     3001 aatggtggat tttccttctc caggacctga tgaagtcacg acaatcgttt tgatttgctg
     3061 accagctgcg gaggcaaact ggatattcgt ccggatcgta cgatattgtt cggcgattgg
     3121 tgaagatggg tttgttaatg tgatcaaact gactacatga gtttgtgctg ctttttgttt
     3181 ctgtgttcgt gccatttttc ttcctcctag actcttgagc ggcttctgcg tgatggagct
     3241 gttttatcgt ttgtagcttt tggtcgtgca ggagctgatc cttttttacg gataccagcg
     3301 ttcaattctt tcgctgacat ttctggaact gttcctaaga ttgggaatcc taatgtatcc
     3361 gtcacatatt tttcatcttt cactgtccga tcaagtaatt cgaataagaa tgccaaacct
     3421 acgcctacca tcattcctaa tactaagcca atcactagat tcaatttatt atttggcgaa
     3481 acaggtgtcg tattggcaac tgcacctgag atgatcgaga ttttatcgac attcatcacg
     3541 tcttttgcgt tctcttggaa aacttgtgaa gttgtattag cgatctgttg agcagtcact
     3601 gcattcgtgc ttattgcctg gatcgagaac atttgtgagt tttctgactg attgacggag
     3661 atcgcatctt tgatttgtcc agcggtcatt ttcacattgt cttctgtttc tagacgatct
     3721 ttgacttcat ttaatactag atcactaacg atcatatctt tatacgtatt gatcatttgc
     3781 aggttcgtat tgacatcatt gacattcgct gtgtctgatt gcggcagtgt cacgatcaat
     3841 tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc
     3901 aagccagcaa acattgagat gatgattgtt gccaagtgtt tgcgtaatgt tccaaaaatt
     3961 tctttcaagc tgattgtctc ttccattttt tctccacttt cctatttatt ctctagattc
     4021 atttgatctt ttaattcttt tttcacacga tcaagttctt gatcgctgac tttttgatag
     4081 gagatattat cttgcatgaa gccttctcct tgcagctgat cttgcttgat cgtaccaaat
     4141 gcctcccgat aatctaaagc gatttttttc atttcatcaa agctcatgtc tgttttcata
     4201 ttcgattcta gagcattcaa aacggattga tagttgctga caccgtttag actcaatact
     4261 tttttcgcta caccttctac gatctttctt tgacgctctt gtctaccata gtcaccattt
     4321 ggatcttcat aacgcatacg agaataagat aatgcctgat ccccgttcaa tgtgatttta
     4381 ccgattgtaa agtcatagcc atcttgtgag aaagtgaggt cattattgac ttcgatacca
     4441 ccaactgcat caacaagttc ttttagccct ttcatattga tagagacata gtgatcgatt
     4501 gggatgtcta catatttttg tacggtatcc atcgccatgg cagctccgcc gaatgcgtaa
     4561 gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt atctcgagga
     4621 atgctgacga tcgttgtctg tttgtcttca ggactgactg tggcaaccat catcgtatct
     4681 gatcttcctt gatcgacccg acctaaatct ccagtatcaa tccccatcaa tagaacagag
     4741 aaagattctt gtttgtccag atcaactggt ttcgttcgtt ctccttgggt tcgttccact
     4801 gattcgtaag ttttctcaat agaatgtgag agatccagat ataattttgc tcctactcct
     4861 acaattgcta atactaatac gataagaact gctaaaataa tcagaaatat ttttttccct
     4921 cttgattttc ttcttcgtct tgccatagtt tcctccgttt gctaaccttg cttgaatcat
     4981 cgctttaact gtcaatagtt tatattgcta tagcccattt gctgagcaac agtttgtgct
     5041 tttgttgcgt atgctacgct tattttttca ttccggatct gctctatttg actgagcgcc
     5101 gtcaaatatt gatcgtaagt agcggtggca gtcacttgac catcctttaa catcgtatcg
     5161 aataactgat ctagttgcgt gacttgcgcg atctgggtat cagcgaatcc tagatattgc
     5221 ataacaaccg ttttccaagg actatcttta aaaaagctca tattttcacg tacacgagcg
     5281 acatctgcac ttgtcaccct ttctttgatg atgacatcgt ctacagcttg ctgccagttt
     5341 gatacatttt tagtaaataa atcgttgaca gctgtctgga tcttcagttt tgattctgca
     5401 ctatctaact gtgtcacgat tgctttcttc tcatcttgga ttgcaacagc ttctttaggc
     5461 aattctgatt ctttgatgtc gtaatcatct gctgaaagtt tgacagagga tagcttattt
     5521 gacaaacttg tgattgtttc ttctgttatt tcagggttta gaaaaatttt ttcttttgta
     5581 ctatacagct tgtctacttc ttttgataaa gaagtcaagg tgtctttttg attggtgatg
     5641 gcagattctg cataattgat ccgttgctga tgttgccgct gattattgat atggtatcca
     5701 aacaaacaga tcgcaatagc acctgcacct accgccacag cagtcaatga cttgacgaat
     5761 ggcttttcca tagattcgct cctaagtatt gatgatagat aaattaagat ataattcact
     5821 tacataaaaa tgacagtttt ttatttaaaa aaacataatt aaactaatat attcacagaa
     5881 atatttgtaa acaagttttc cctatataat cataccacaa actgctttct tgactaggga
     5941 aatttacgat ttaaaagtaa ataaaacatt aataagataa caattaatcg attttacttt
     6001 tatttcctta a
//
LOCUS       DACEMO010000153         5992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEMO010000153 DACEMO010000000
VERSION     DACEMO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.158"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(249..1457)
                     /locus_tag="IW728_002199"
     CDS             complement(249..1457)
                     /locus_tag="IW728_002199"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1025541.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(1569..1901)
                     /locus_tag="IW728_002200"
     CDS             complement(1569..1901)
                     /locus_tag="IW728_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1025542.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2049..2924
                     /locus_tag="IW728_002201"
     CDS             2049..2924
                     /locus_tag="IW728_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1025543.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3006..3587)
                     /locus_tag="IW728_002202"
     CDS             complement(3006..3587)
                     /locus_tag="IW728_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1025544.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(3593..4852)
                     /locus_tag="IW728_002203"
     CDS             complement(3593..4852)
                     /locus_tag="IW728_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1025545.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(4854..5900)
                     /locus_tag="IW728_002204"
     CDS             complement(4854..5900)
                     /locus_tag="IW728_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025546.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1674 a         1237 c         1020 g         2061 t
ORIGIN      
        1 tgtttgatcc aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta
       61 aaataaaatt ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat
      121 ttattcttgt actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt
      181 ttcttctaat gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct
      241 agtgtttttt aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt
      301 tttattcttt gttctacgac ggctttttca gctaattgca aagcttcact aacccactta
      361 ccaggttttt ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat
      421 gacttgatag gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca
      481 aaatagatca tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg
      541 aatggttcca aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct
      601 ttaatcattt ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt
      661 agatccagtt ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat
      721 ggtaattcag caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg
      781 tagcactctg tttcaataaa tgcatggatt cccgcggccc gttcatttcc caagagcatc
      841 ttgatgaatt ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct
      901 ttgatggcgt agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac
      961 ccgcgcatca ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc
     1021 aagatctgtt cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct
     1081 ttcaaagcaa acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga
     1141 acgaattcga catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg
     1201 gtgatctcat attgttcatc attttctaat accaacacag ttccatgttc gatcccaata
     1261 tctatcgttt ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc
     1321 acatcatgga ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct
     1381 tcatatcctg cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt
     1441 aattgtttta atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac
     1501 tcagttgcat ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt
     1561 catcttgttt actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc
     1621 atgttttttt caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta
     1681 acgaataaaa catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg
     1741 cttttttctc cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat
     1801 cgagccatct gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc
     1861 acttcctgct gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta
     1921 tcttaccaaa actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat
     1981 gctacattta ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg
     2041 gaattcatat ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa
     2101 gtatttatgc atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag
     2161 tctcagggat caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt
     2221 tattaatcaa catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg
     2281 cctatactgt tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc
     2341 cactagaaat caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg
     2401 caggtgtagg aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta
     2461 tcgctcggat tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg
     2521 atatcatcgt cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt
     2581 tgattgtttc gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg
     2641 caaaaggtct acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg
     2701 gtctgcaaag aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa
     2761 aaatcatcta tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg
     2821 agattgacga aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct
     2881 ttacttattt gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat
     2941 aggctatgag gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt
     3001 tttaactaat tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc
     3061 tttcggttaa cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct
     3121 tgatcgattt gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat
     3181 cgttcaatta attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg
     3241 tcttctaaga tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca
     3301 tataaaggct ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat
     3361 tcttttccct gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc
     3421 agtcctcgat ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca
     3481 tgattggcaa gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg
     3541 accatccacg ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct
     3601 ctgttaggtc atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat
     3661 aagtcaataa ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact
     3721 ctttcaagaa atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg
     3781 caacggtaaa gtcttctaat tcattataag catgggctag gttccattct gcataaggat
     3841 tagcagtttc ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca
     3901 agtaaagatt acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt
     3961 tcagtaaaag ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca
     4021 ggtgataaag ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg
     4081 cttcttcaat catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat
     4141 aatgcggatt aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca
     4201 attgaagatt cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg
     4261 cttcttctaa aggtacgata gctgcttcga attctccttg catacttaag gattgtccta
     4321 aacgttcctg catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat
     4381 aaaccactgc tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact
     4441 ggatcaatgg ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt
     4501 cagggattcc gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat
     4561 cactttgctt atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga
     4621 ttgcaatttc tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt
     4681 gttcaaaaat ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt
     4741 cagctaatat atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg
     4801 acaagtcttc atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc
     4861 cctcttatct cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg
     4921 tttggatgtt ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt
     4981 ttttctgcca aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag
     5041 tggattggat agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa
     5101 ggatatgctt ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta
     5161 gcagaaagta gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa
     5221 tttgacaggt tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca
     5281 aacggccgag tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa
     5341 cgatgatcac aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc
     5401 acctgccgct cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc
     5461 agcactacac gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga
     5521 gaacggatcg atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa
     5581 aaacctgcca gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt
     5641 tcgggatagc gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga
     5701 gtgttggcac aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta
     5761 tctgcaaaca aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa
     5821 agttctctta gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct
     5881 acttgataat caagttccat acgcatccct cctcttctta tttttatttt accattagta
     5941 agcaaattct agaaaagata agagcaaagc tcacctcata acttggaaat aa
//
LOCUS       DACEMO010000154         5984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEMO010000154 DACEMO010000000
VERSION     DACEMO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.159"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(151..2058)
                     /locus_tag="IW728_002205"
     CDS             complement(151..2058)
                     /locus_tag="IW728_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1025547.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2188..3063
                     /locus_tag="IW728_002206"
     CDS             2188..3063
                     /locus_tag="IW728_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1025548.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3143..4117
                     /locus_tag="IW728_002207"
     CDS             3143..4117
                     /locus_tag="IW728_002207"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1025549.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4306..5442
                     /locus_tag="IW728_002208"
     CDS             4306..5442
                     /locus_tag="IW728_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1025550.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5579..5923
                     /locus_tag="IW728_002209"
     CDS             5579..5923
                     /locus_tag="IW728_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1025551.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         1879 a         1112 c         1243 g         1750 t
ORIGIN      
        1 gaaactaagc agctttttca caacctctaa tacatggtat tccagaagta acttcttcga
       61 aaaataagcc gaaatgtcac aaaaatttga gaagcgattt tcccaaattc cttcttattt
      121 cttgaagctg accacttctg gcacaacctc ttaccagatt cgcactcttt tttctggcgc
      181 tagatacata gcgtcttgtt ccgtgatacc aaatgtttca tagaattcgt caaggttttt
      241 tggttgcacg tttgctcgga gtttagcagg ggcatggaca tcgatttgca gtaaaagttg
      301 ttggtattct ttttttgctt ttgtacgcca aatcgttgcc caattgacaa agaattcctc
      361 gatagaaaga tctgattccg tttttgctgc ttccaatgca cagcttagac cgcctgcatc
      421 agcaatattt tccgaaacgg ttaattttcc gtttactttt ccgtcagcaa aaggcagacc
      481 atcgaattct tcgatcattg cttgtgcttt gttttgaaat tgtgtaagat cttcttctgt
      541 ccaccagttg ttcaagttgc catattcgtc aaataaagcg ccgttattat caaatgcatg
      601 ggaaatctca tgagcgataa cagctccgat tccaccataa ttagcagagc ttgattgttc
      661 tagactgtaa aatggtgctt gaaggatcgc tgccggaaag acgatcacat tccgaaatgg
      721 atgataataa gcattgaccg tgtcagcact catttcccat tctgaacgat ccactggttt
      781 gttccatttg ctgaaacgat cttttaatgt gagtttgctg aaaacaagag tatttgaaag
      841 caaagtacca ccctgatctg caggtactgt tttgaactgg tcgaataatg ctgggatctt
      901 atcggggtag cctacttgga tacctaagtt atttaatttt atgacggctt tttcttttgt
      961 tgcatcccca agccagtcat tttcttctag acgtttttta taaacggcga tcattttttt
     1021 caccatttgt tctacgtctt ttttagcagc ttcgccaaaa tatttttttc cgtagtaatc
     1081 gccaactact tgatcgaatt gtccagaggc aaggtaatac gctgcttttt tctgaggcat
     1141 tgcttcgtct gttccagaca atacgcgaga atagattccg ctgacttggc ggaattcttc
     1201 tgatagataa ccactaagag aacgtactgt tttaatgatc atccagcttt tgaataacgg
     1261 aaaatgagct tctgtcagga tgtcattcaa atgatcgaag tacactggat cagttacgat
     1321 cactttatct ggagttgtcc caatcaaacc cgtgacaagt tttttcaagt ccagtttatc
     1381 tgtgtattta ataaatgtat caaaagattg cggattatac attttgctgt aatctgcgtt
     1441 ttcttctgca ctttttacat gaggagcaat caatttatca aattgcactg cttgttccgt
     1501 gattgcttct gcctgacttt tttctttgcc ggcaagctct aataactgaa cagccatatc
     1561 aaagaaaacg cctattagtt gtgcaccgtt gggatggtct gatgcataat acgtcttatc
     1621 tggaaggaat aaagaaggcg gataagcaaa gaaggcgttc gtttgcgcat tcttcatatc
     1681 tgcatctaca tccagattaa atggcaatgg taatgtatcc aacagccatt ctggaaattg
     1741 cttattcaat tcgtcgaaag actgcaatga ttcaactttt tcgattatag gttgaagcgg
     1801 ttgagcatgc aactcatttc ttcggtcata atcgtttgcc agttgataat atgcaaggaa
     1861 atgtttcatt ttttctgaag acactttttc tggatgggca agcagatcgt ctgtttctcc
     1921 catcaaaagt tggtcgatcc cgtcaactaa atcttggaaa ccgcctgtag ctggtttatc
     1981 tgcagggatt tttgctgttt tttgccattc ttcattgact gcttcataga aatcttgttt
     2041 caatcgttct ttgttcattc atcgatcgct ccttttttgt ttcgtgtgcc cattatctta
     2101 caaaatgaag gaattaacaa atagaacata aaaaattgaa aattcgaaca atcaaactga
     2161 tttgtttttt tcaatccttc acttataatg aaaaaaagga ggcgatccca actgaatcaa
     2221 gtagtggata aacctttata tttcaaagta gataaaaaaa ttaaaaaatt agccagcact
     2281 cagcagctgc agtcaagaaa aagtaaattg ctttttttag ctctggtttt tgaagatcaa
     2341 agttatgtga ttatcgatca atcaggacat ccggtagaat attctccagc aaaatatact
     2401 tatcaagagg gtctttcttg taaaaaatgg aagctgatca atgaagagcc aatcgagtta
     2461 tctagatgga tcaacagaaa agaagatatt cccgttttga tcgaagaaaa aaggtcagga
     2521 aaggaactcg caaattgctg ggtgggattg ccagaagaac gttttttgag gtataagaaa
     2581 tgggctaccc cctcaggcta tctttgtggt acttacgcgg cagcagttct ccttgcctat
     2641 tatcaagact atcgtaaggg gtggatgctt ccaaatgaga ttcgcaagaa aaatacggct
     2701 gattcgttgg ttttgacgaa ggcattgcgt agccagatcc aaccactagg acttcctacg
     2761 attccttttc aggttagtac agggatttct agttttttaa agaagaatgg caatcatgag
     2821 cgtgcacgag caactcttct aggttcatgg cagcgagcta caaaacgtat acgtgaaggc
     2881 aaacctgtga tgattggtat tttgaaagta cttggcagta catatggcaa tcattgggtg
     2941 acagcgtacg cttattttga aactgaaaca ggagaacgtt attacaaagt acacgacaac
     3001 tggggcgatt atcataaagt cattcctgcc agttggagta acggaacagt ttcacttcct
     3061 taagaatttg ctgaaattaa cagaaaatct tggaaaaata tgttaaacta tcgaagaata
     3121 gagaagtttt ggggaggact acatgagtaa taagtatcag gatgacacgt tgaagatttt
     3181 tagtttgaat ggcaaccgtc cattagctga aaaaatcgcg aaagtgttcg gaacagaatt
     3241 aggtaaaagc gtcgtgaagc aatttagtga tggcgagatt tccatcaata tcgaagaaag
     3301 tattcgaggg gatcatgtgt atatcgttca atcaacgaat gcacctgtca atgattacta
     3361 tatggaacta ctgatcatga tcgatgcaat gaagcgagca agtgcgaaaa cgatcaatgt
     3421 cgtattgcct tactatgggt atgctaggca agaccgcacg gcgaaacctc atgaaccgat
     3481 cactgcgaaa ttgatcgcta acttgatcga agaagcaggg gcaactcgag tactgacttt
     3541 agatctgcac acggttcaag tacaaggatt ctttgatatt cctgtagata atttatttac
     3601 gatgccgttg tttgctcatt attaccgtca attaggtctg acgggagatg acatcgtagt
     3661 tgtttcgcca aaaaacagcg gtgttcagcg tgcacgtagt ctttcagaat atttgaacag
     3721 cacattagcc atcgtagatc atgcagatga tgcttcaact gagggtggtt atgtgatcgg
     3781 tgatgttcaa ggaaagacat gtatcatggt agatgatatc ttgaacacag gagcaacgct
     3841 tgcacgagca gcaaatgtat taaaagaaaa cggcgcaaaa gaagtatatg cctgtgcgtc
     3901 acatggatta ttgtccgatc atgcgaaaag tattttagac aatgcaccaa tcaaagatat
     3961 ttgtatcact gattctgttt tcacagaaga aaatcgtcat ccagaaaact taacaatcgt
     4021 tacatgttca gaattaatgg gtgaagcggt caaacgcatc catgaaaata caccgatgag
     4081 tccgttattc cgtctggaag aaaaagaatt cgaataaaca aaaaaaagag ggaaagaagt
     4141 tgtgacagaa gtgagcagct tcaaggagct gcgacaagac ttttgtcaca gctccttttt
     4201 tctatgccca tgaaacgtcc tctagacaaa aatgagctct tctttggttt gttcgctatt
     4261 ttatgctaga atgtgcctag caataatttg gaagggatga atgaagtgaa aactatttat
     4321 ctggatcatg cggcaactac ccccatgcat ccacaagtaa ttgaagcgat gacggcaatc
     4381 atgcaagaca cgtttggaaa tccttcaagc atacatggat ttggtcgcca cgcgcatgaa
     4441 aaattggaaa cagcaagaca aatcattgca gacagtctgc aagcaaaacc tcacgagatc
     4501 atctttaaca gtgggggaac agaaggggat aatacagcta tcttagaaac agcgttctcg
     4561 cgtcaaaaag aaggcagaca tctaatcact acagctatcg aacatcctgc tgttcttaat
     4621 accatgaaat atttagagac aaaggggttt gacgttacat atctgcctgt agatgaaaaa
     4681 gggaatctgt cgatcgaaga ggtcaaaaaa gcgttacgag aagatacgat cttggtctct
     4741 gtgatgtacg ggaacaatga aatcggaaat ttgatgccaa tcaaagaaat cggtgaactt
     4801 ttagcggatc atccagctta tttccataca gatgcggtac aagcgtacgg aaaccaaacg
     4861 atccatccag aagaattagg aatcgattta ttaagtatct ctgctcataa aatcaatggg
     4921 ccaaaaggcg tcggtttctt atataaacga gacggagtaa atatccctgc attgctttta
     4981 ggcggggagc aggaagaaaa gcggcgtgca ggtacagaaa atttagcagg gatctggggt
     5041 atggcaaaag cagttgaact gctgtcacct gaagaacgtg aagcacgtaa caacaaatat
     5101 aaaggattcc agcaattgat tcttgataca ttagaagcaa acgaaatccc ttatcaagta
     5161 aatggtgatt taacgaataa actccctcat gtgttgaatc ttcgcttaat tggcgaagca
     5221 aatgatttgc tactgatgaa gcttgatctt aatggggtcg ctatttctac tggttcagct
     5281 tgtacagcag gaaatatcga gccttcacac gtattagaag caatgtatgg agaagataca
     5341 ccgatactaa aagaatccat ccgtatcagc tttggtttgg gaaataccga agacgacatc
     5401 cagtatttcc ttgatcaatt agttcgtgct gtcaaaaaat aactagtgaa gtcattctta
     5461 tatgaacaga tgtctgagag aacgccttca ttttttaggg aacttaactg aaaaacctag
     5521 taaaaatcag gataaacttt tataataaag agaaactaat tttatcgagg tgaaaatcat
     5581 ggcatttgaa acaacagcaa cggtactggg cgcaccaatt agctatcgtt tgcatcctga
     5641 tgcaaaaaga tatacattgc gcgataatgg atttacagaa acaaacggag gcaattatca
     5701 attgatccga ccattagatg ccacgccgca aagcaaagaa ggattcaaat taaaaatcac
     5761 tgtatcaaaa gatattcaaa cattgaaaat gtcgatcact acagcaaatg gattgaaagc
     5821 agtcaatatt ttcaaagatc caaaacagaa aatgagccaa gataaatttt attttctgat
     5881 ggatgggatg attagccgtg ggttattcga aaaagtcgaa tgatctgtgg atataaaaag
     5941 acaactagtt ttgaaacggc ctattgttag atgactaact ttgt
//
LOCUS       DACEMO010000155         5926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEMO010000155 DACEMO010000000
VERSION     DACEMO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.160"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(68..988)
                     /locus_tag="IW728_002210"
     CDS             complement(68..988)
                     /locus_tag="IW728_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1025552.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            1153..2166
                     /locus_tag="IW728_002211"
     CDS             1153..2166
                     /locus_tag="IW728_002211"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1025553.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(2277..3032)
                     /gene="fabI"
                     /locus_tag="IW728_002212"
     CDS             complement(2277..3032)
                     /gene="fabI"
                     /locus_tag="IW728_002212"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1025554.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            3252..3692
                     /gene="fabZ"
                     /locus_tag="IW728_002213"
     CDS             3252..3692
                     /gene="fabZ"
                     /locus_tag="IW728_002213"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1025555.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(3807..4568)
                     /locus_tag="IW728_002214"
     CDS             complement(3807..4568)
                     /locus_tag="IW728_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1025556.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            4757..5851
                     /locus_tag="IW728_002215"
     CDS             4757..5851
                     /locus_tag="IW728_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1025557.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         1876 a         1063 c         1181 g         1806 t
ORIGIN      
        1 gacaaaagtg ttctttactt ttgtcccacg ctctttttag tgagattcgt tgcatcgttt
       61 gtccaaatta ttttccgaca gcaattggac tgttcggaat cgtgaaacca gaagctgtca
      121 attctttgac ataggagctc aagaactctt cttttaaggc atattgtttt ccgttcaatg
      181 cgtagcagac cgtacgaatc gcaaaattgc tgtttccaag atccactaat ccaaagatag
      241 aaggttcagt ttggagctct tcttggtatt tttcagctaa tgtacgattt actcgatcaa
      301 tgatctcata gattttgtca tatccttctt caggaacaat ccgaatatct aaaagtactt
      361 gcatatttgc acgagagaga ttgctgatcg tggtgatgtt tcgattcggg atgaaatgga
      421 cagtaccatc cgttgctttc agctgaaggg tacggatacc aacagatacg acggttcctt
      481 caatcgttaa gtttgctagc ttgatgtagt ccccaacatc catctgttgt tccatgataa
      541 taaaaaatcc ggtaataaca tcactcatga acccttgggc accaagaccg attgccacac
      601 ctgcaattcc ggcaccagcc aaaagtgatc cgatcggcac accgatagca gataaaattg
      661 catagataaa aaagaaacca agggtataat gaaaaatagt atttaaaaga ctatgtaaag
      721 tctttaagcg gctctcgttt agaatggctt ttttagaata ctgattaaaa gacttatcaa
      781 tcagatattt tcccacacgg tgaagaatcc aaaaaaggac gatcaaaaaa atcagatata
      841 aacttttttg gataaaggca gccaaaatgg cgtcccaatc aatcccgtcc caaaaccgct
      901 gaaacgcatt tactttgctg acagcttgat ctgtcaatgt ctcgccagaa gagtctgtcg
      961 taccggcagt tgctaataat gaaaacataa aatcctcttt tctaaaattt gattctttct
     1021 gtattgtacc aagaagtttt caaaaaagga aatagcaaaa ataactataa tgaaggaaaa
     1081 aagctgaaaa taaaatgaca aataatatgg ataatgataa aatgtggaga aaataacgaa
     1141 ggtggtaagt atatgcaaat cgattggaac aatctaggtt tttcttatat caaaactcct
     1201 tggcgtttta ttgccaaatg gaaagacgga caatgggaag aaggagaatt gacagaagat
     1261 aattacctga cgattcatga aggctctcca gcgcttcatt atggtcagca atgttttgaa
     1321 ggtctaaaag cttatcgtcg aaaagacggc aaggtcaatc tctttcgacc tgaacaaaat
     1381 agtcgacgga tgaatcaaag tgctagacga ttgctgatgc caatggtccc tgaaaagatg
     1441 ttcgttgatg ctgtcaagca agtggtaaaa gcaaatgaag attttgtgcc gccctatgga
     1501 acaggcgcta cattatactt gcgcccttta ctgattgggg tcggtgaaaa cattggtgtc
     1561 catccagctc cagaatattt atttgtcgtc ttctgtacgc cagtaggagc gtatttcaaa
     1621 ggtggactaa aaccaacgaa ttttattgta tctgattacg atcgtgctgc tcctaagggg
     1681 actggtgcag caaaagtcgg cggaaattac gcagcgagtt tacttccagg aacagaagca
     1741 aaagaactgg attactcaga ttgtgtttat cttgatccag ccacacatac gaaaatcgaa
     1801 gaagtaggtg cagcaaattt ctttggaatt acaaaagacg gaaaattcat cactccgcaa
     1861 tcgccctcta tcttaccaag tatcacgaaa tattccttgt tgcagttagc tgaagaacga
     1921 ctaggactgc aagcaattga aggagatatt ttcattgatg atctagatca atttgcagaa
     1981 gctggtgcat gtggtacagc ggctgttatt tctccaatcg gtgggattta tcacaataac
     2041 aatcttcatg tattttatag tgaaacggaa gtaggaccag taacaaaacg gttgtatgat
     2101 gaattgacag gtattcaatt tggagacata gaagcaccag aaggctggat tgaagtcgtg
     2161 gaataatttc aaagtaatga gttgaaaaaa aacgtaaaaa gaaaaaatca ggaagagact
     2221 ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac aaaagtttat
     2281 gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct ggaaagtggg
     2341 ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg ctgatcaggc
     2401 gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt gacagctaat
     2461 gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga gaactcagct
     2521 gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat gttataattc
     2581 gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt tggtgcaaga
     2641 aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc ttgagctaat
     2701 tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt gttggcgtaa
     2761 gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc attcattgct
     2821 ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc ttcgccgacc
     2881 aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat ttctgcccct
     2941 tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc gacgcccata
     3001 atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt ctgataaaaa
     3061 tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat ttcttaatta
     3121 ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa gattgctaaa
     3181 tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg cctgaaagaa
     3241 ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg attcctaatc
     3301 gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa aagatcgttg
     3361 cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct gacaatccag
     3421 tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata cttatcctaa
     3481 aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag gccaaattcc
     3541 gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc aaaatgaagg
     3601 gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc tgtgagtgcg
     3661 agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag caaaacaaaa
     3721 ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat acgttgtcat
     3781 ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag tatattgatt
     3841 ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat atttgcaata
     3901 gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg gccatctagt
     3961 gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc gtcttttaaa
     4021 agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc gttactgtta
     4081 ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg gttgttgatc
     4141 tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc tttaggatcg
     4201 ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt gtagtaaccg
     4261 tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg aaataatttg
     4321 attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc ggcgacatct
     4381 ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt cgtgctttcc
     4441 gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt gttgtttgca
     4501 gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat aactttattt
     4561 ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat aacaaaatag
     4621 agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga aaaagaaaag
     4681 gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac gaaaagatgc
     4741 aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac ggtcaacatt
     4801 tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac gggtttttac
     4861 cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta aagaagtagt
     4921 tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag cgttatttga
     4981 acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg cctacggggt
     5041 ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca atccttttgg
     5101 tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca tcatgagtta
     5161 ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc gatactctgg
     5221 tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata atttgaatac
     5281 gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc aagatggctt
     5341 gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc atttaatcct
     5401 tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag aagaattgaa
     5461 agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata cggaagaagg
     5521 tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc gaggggatcg
     5581 ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata aagatttccc
     5641 gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg aagggtacgg
     5701 tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg gacgtgtcaa
     5761 tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga aaccatggtc
     5821 aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa ataatccgct
     5881 agaaatagcc agttatttct gcttttttat tttgtttttt tatagg
//
LOCUS       DACEMO010000156         5877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEMO010000156 DACEMO010000000
VERSION     DACEMO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.161"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            641..1375
                     /locus_tag="IW728_002216"
     CDS             641..1375
                     /locus_tag="IW728_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1025558.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IW728_002217"
     CDS             1391..2560
                     /locus_tag="IW728_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1025559.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IW728_002218"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IW728_002218"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1025560.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IW728_002219"
     CDS             3650..4576
                     /locus_tag="IW728_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1025561.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IW728_002220"
     CDS             4595..5845
                     /locus_tag="IW728_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1025562.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1204 g         1751 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaagt
//
LOCUS       DACEMO010000157         5827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEMO010000157 DACEMO010000000
VERSION     DACEMO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.162"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..665)
                     /locus_tag="IW728_002221"
     CDS             complement(93..665)
                     /locus_tag="IW728_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1025563.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(672..1334)
                     /gene="rpe"
                     /locus_tag="IW728_002222"
     CDS             complement(672..1334)
                     /gene="rpe"
                     /locus_tag="IW728_002222"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1025564.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1350..1865)
                     /locus_tag="IW728_002223"
     CDS             complement(1350..1865)
                     /locus_tag="IW728_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ1025565.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1867..2151)
                     /locus_tag="IW728_002224"
     CDS             complement(1867..2151)
                     /locus_tag="IW728_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1025566.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2185..3513)
                     /locus_tag="IW728_002225"
     CDS             complement(2185..3513)
                     /locus_tag="IW728_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1025567.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3527..3982)
                     /locus_tag="IW728_002226"
     CDS             complement(3527..3982)
                     /locus_tag="IW728_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1025568.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3985..>5827)
                     /locus_tag="IW728_002227"
     CDS             complement(3985..>5827)
                     /locus_tag="IW728_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1025569.1"
                     /translation="YQDIELLEDYVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVEN
                     YLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGE
                     VKLQSSAEGISFTGNEQAIQRAIKQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDL
                     LFNDYSELTEKVSDYYVRSLISTLVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVAN
                     GLIEELKAKLNLPFYDADKEYLCRQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEK
                     IDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEE
                     EFDVTLTDDEVSLILIHFQIAIEDQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDN
                     VEISTLDKFHLAEIGEVDLIISSLSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIE
                     EPSRFIQNELIPKAVKVPTISKFVQSSLVKTGVQVESKEECLDLMISELEANSLVTEE
                     FRASIFSREKMGNTNLESGVALPHANPSMVIESNIFIVALKNAINWGDRKVKLVIMVS
                     LAEEDVSEIRGVYEELYQFIGQKEMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         1893 a         1134 c          822 g         1978 t
ORIGIN      
        1 ttattaagtt atgtctaatc aaataaaaaa accattctca tatttgctga ataattagca
       61 tttagagaat ggttttttat ctttaaaatc tactactcaa aatctgcatg tcgagcaaac
      121 atcgtttctg agctttcacc tgttttatcc attaaagata acactaaact atcaaaggtg
      181 agaaatgcta attgttcaaa acttgaaccc ataggttgtg aaaaagtatc ttcttctcgt
      241 tgattttcat ctttggtaat acctggtaat acaagaacag aatctgctaa ttgaccaatc
      301 gacgagtctt ttttcatggt aactaaagca atgcttacac cactttgatt tgctttggcc
      361 gctaaatttt ttaaactacc tgtttcacct gagccagaac agataattaa taaatctcca
      421 ggttgcgaat gtggtgaact aatttcaccg acaaggctga ctgaaaaacc taaatgcatt
      481 aagcgattcg taaatgcttg catggcaatt ccagagcgtc ctgcaccagc tagaaagatg
      541 tgtttagctt gaacaatttt ctcactaaat tgttctaact ctacagaatt aatacgtttt
      601 gagttatttt gtaattctaa gataatcgct tctaaatagt ttttattttg gtctttattg
      661 gtcatgatct tttacttcac ttcctctaat gattgattta gtttatcaat ttgttcttgc
      721 ggggtatttt tcccattgaa gatgaaaccg cctgatacaa aaatatccgc ccctgcttta
      781 gaacaaactt taatcgtttg attatcaatc ttgccatcta cttcaatatc atacgtataa
      841 tcattttgtt tgcgtattac atctaactgt tcgattttaa ctactgtctc ttgaataaaa
      901 tgcttattct ctgttcctgg attgattgtc attactaata cttgatcgac taaagataat
      961 actggttcaa taaaactgac tggtgttgca ggattaaaag taatccctac ttttgctcct
     1021 gcttccttga ctaactgaat gaccttcatt gcgtgtttgg tagcttcaat gtggaaggta
     1081 acaatatctg ctcctgcttc tacaaatctt gtgacaattt tttctggtgt atcaatcatc
     1141 aaatgaacat caaacacaac gtctgttagt ggacgaatat ctttaataac acttggtcca
     1201 aaagccatat ctggtacaaa acagccatcc attacatcaa tatgcaaata atcaacattc
     1261 ttttctttta agatttcaaa taactcttta acttctgcga taggtgcatt taacaatgat
     1321 ggagcaattg tcattctcta ttcccccgtt tattctccca taatcatgag attgattgtt
     1381 ctagttcgct ctcttagttg atcccaatcg acaaaataga cacggcccat ttctcctaat
     1441 aaaagtttac cttcttgaac aattaaagtc attgagttgg ttccataaat agatgaacgt
     1501 aaatgtgcat cggtatttag catgacccat ttttctgcag ggtaattggg atcagataat
     1561 cttgttccaa aatcaatgtg ttttggcccc ggactgttgt attgattttc actcgtcatt
     1621 tttggaataa tcgtttccat tacattgttg atgtctacat gtaaataatc atcaccaaaa
     1681 aagtttgtgt catgcatcgt ttcttcaaaa aagagtgagc aagttgtatg cgggctcgaa
     1741 attactaaca ttccattctc aatacctgct gtatcaactg tatctaaaag ttcttttcga
     1801 atatcatgat acgaaggacg ccctgaaact gtttcaatag tcaaagtgtc atgatgaaaa
     1861 cgcatattaa acacctaatc tttcttttac atctgcataa atggcatctg taccaatacc
     1921 tgttaataag cccaagccat tcgttacttt agctttaatt acatcaggga caattgttgt
     1981 acttactaca gcatcatatt catcaatttt atttagctca tcagcaattt tagattgata
     2041 aattttcact tgatctgtta atccttcttc ttccaaccat tttgataatt ttgctactgc
     2101 aattgtagaa gtagcatgac ctgctccaca aataactaat aattttttca tattgatctc
     2161 ttctttctta ttttaatttt atgattaagc tttcttcact ttgttcatgt agatagaagc
     2221 acctactaaa acaactccta atccacctat tccaattaca ccaaagtaag atgctaataa
     2281 aacaaacaac cctactggcc ataatccaca taacaacata gttactaatc ctgctgtccc
     2341 cacatcgaaa cttgctaatg taaacacatc agtgataatt ggtgccaacc atgttgccat
     2401 atataatgtt gtaacaacat acaacgaaca tcctactaat gatctcacaa tatttccttt
     2461 aaaataagga atcattaaac agatggcgaa agcaaagaat gctaaatctc caaaaggtaa
     2521 tactttattt cctggaagaa ttagtgctag caataatgag attggtacaa gcaatagaga
     2581 agttgacata atagttggat gacccacagt taacgctgca tccattccaa tgtttacttg
     2641 gcgaccattt aactttttat tcgtaaattc tttcgccgct tctgcaattg gcattaaacc
     2701 ttccataaac attgctacca ttttaggcat aatcttcatc actgccgcag tcataattcc
     2761 taagtttcca attcctgcta cgtcatagcc tgccataata cccactacaa taccgataat
     2821 taggccaata acaactgtgt cacctaaaac accgaaacgt tttgttaatg tttctgcatc
     2881 tgcatctatt ttattaaatc caggaatacg atcaaaaacc caatttactg gtttggcaaa
     2941 tagtactcct gataatccca tcatatgtgt gatagagatt cctggatagc cgaaaaattc
     3001 gctaacagtt ttttgtgtac gatctgctaa gaaccattgt aaaattactg cgacaatcgc
     3061 gccaattaag ccaaaccaaa agtttcccgt aacagattga gtaatcatac ctaggaaaat
     3121 tgcgaaccaa aagttccaaa tatcaacatt caatgttttt gtccaaccca aaaccacaaa
     3181 tatgaagtta agaattaatg aaattggaat cattaaaatt cccaatgatg tagagtaagc
     3241 caaaggtcca ccaacaccta cgccaatatc taggatattc aggccaccac cgtattgttc
     3301 ccccatttgt tgaattgctg ttcctaaacc atctgataat aaacttaaaa ctaatcctaa
     3361 accaatagaa ccgataccaa cagtaacgcc ggaaacaaat gctttaccag gttttaatcc
     3421 aaataatatc ccgattacaa aaataatcag tggtaaaaag acgtttgacc ctaatccaat
     3481 aaaccattca aaggcattta atacaatatc catctctcta ttcctcctaa taaactcctg
     3541 cagaatgtac taactcaata aatgcttctt tcgtggtaca tgcaagtaat tcatttactt
     3601 tttcttcatt ttgaaatagg ctaactaagt tttgtagtgt atcaatttgt tcatgaggtt
     3661 gtgacattgc caacataaag actaaacgta caggtatttc atgagattta tctgtcatat
     3721 ctgaaaatac cagtggttcc tttaatgagg caaaacagat ttgagagcta tttacatgtg
     3781 cggaatctgt atgtgggata gcaaacccaa tttgatttac ttctaatcca gttggaaatt
     3841 cttgttcacg gttggtgata ccttctaaga aactatcttt cacgcaacca gcgtttttta
     3901 attgttcaga cattacttga aacaattctt tttgatcttt cacttctaat tgaaataaac
     3961 ataattcttt atctagatac atttttattc tcctttttgt tgaaaaattc ttagtaattt
     4021 ttcgctatca tgaacttgta acaagcgatc gaccatttct ttttgcccaa taaattggta
     4081 aagttcttca tatactcctc taatctcaga tacatcttct tcagctaaac taaccataat
     4141 gactaatttg acttttcgat cgccccaatt aatcgcattt tttaatgcta caataaaaat
     4201 gttggattca atgaccattg atggattcgc atgcggtaaa gcaacaccac tttctaaatt
     4261 agtattcccc attttctctc tagagaagat agaagcacga aactcttcag taactaatga
     4321 attggcttct agttcggaaa tcatcaaatc caaacattct tctttgcttt ccacctgtac
     4381 tcctgttttt actaaagatg attgaacaaa tttagatatg gtgggaactt taaccgcttt
     4441 aggaatcagt tcattttgaa taaatcgcga aggttcttct attaaatatt tagtataagc
     4501 ttccataata ttcatataat ctgttttact taccaatgga gataccctta ctaagggtat
     4561 accacctgca tcaaaggaaa gagaggaaat aatcaaatca acttccccaa tttcagctaa
     4621 atgaaactta tctaaagttg atatttctac attatctttt gctggtaaaa accgtcgaac
     4681 tttattgtaa attaattgcg cagaagaaat accgtattgg caaataatga tgatattatt
     4741 tgctttggac tgatcttcaa tagcaatctg aaaatgaatc aaaattaagg aaacttcatc
     4801 gtccgttaaa gtcacatcaa attcttcttc aaggatggac atggcgtacc atactatgct
     4861 aaagagttct gaatattgag atttgatatt ttctaataac ggattttgaa ttttaatacc
     4921 tttttttaaa cgaaggacca ttgctggcac atgatacaat aaggattgat ataatcgatc
     4981 attttgagtc aaatcaattt tttcaatctc actcattctt tcaatcaatt tatgaacgct
     5041 catagcgtac tcttcattgc tggtttttaa attatttgtc acacgatgtg caaatagttg
     5101 acgacataga tattctttat ctgcgtcata aaatggaaga ttcaattttg ccttcaattc
     5161 ttcaattaaa ccatttgcca caatgtacgt ttccatatag cgaatgctat tgaagaggaa
     5221 ttcttcttca acagttaaat gatgaccttt cagtaaacgg ttacattgga taactaaagt
     5281 agaaatcaat gaccgaacat agtaatcaga aaccttttca gtcagttcac tgtaatcatt
     5341 gaatagcaaa tcatgaatga cactaataac ttttgaatca aacaacatat ttaaaacttg
     5401 aaaaaatgtt tttgattcca tgtcattcgc ataatagaat acaagttgct tgattgctcg
     5461 ttgaattgct tgttcattac cagtaaaact aattccttct gcagatgact gcaacttaac
     5521 ttctccactg ccaataattt tattgataat tctcaggtca ttgtataacg atgttttact
     5581 aaccataaaa ctttgagaca aatgagttaa agaaacaggc ttatcaccaa acaaaacttc
     5641 tttaatgatc tcgacacgtc ttttttctgg agaataaata tctttttcaa gataattttc
     5701 taccgatgaa atcaaatgtt caacgttttt cctatcacct atgagtttga tacccaaaga
     5761 aggtaatcgc tcaattgtta cttggtaatt aactacataa tcttccaaca attcaatatc
     5821 ttgataa
//
LOCUS       DACEMO010000158         5797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEMO010000158 DACEMO010000000
VERSION     DACEMO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.163"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            107..391
                     /locus_tag="IW728_002228"
     CDS             107..391
                     /locus_tag="IW728_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1025570.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            394..1311
                     /locus_tag="IW728_002229"
     CDS             394..1311
                     /locus_tag="IW728_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1025571.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            1304..2086
                     /locus_tag="IW728_002230"
     CDS             1304..2086
                     /locus_tag="IW728_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1025572.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            2087..2767
                     /locus_tag="IW728_002231"
     CDS             2087..2767
                     /locus_tag="IW728_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1025573.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            2760..3656
                     /locus_tag="IW728_002232"
     CDS             2760..3656
                     /locus_tag="IW728_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1025574.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            3851..5500
                     /locus_tag="IW728_002233"
     CDS             3851..5500
                     /locus_tag="IW728_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1025575.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
BASE COUNT         1821 a         1011 c         1264 g         1701 t
ORIGIN      
        1 gaagaaggga ttgtgaaaag tcttttgtca taacctctta tttggtagct aattgtaaat
       61 cccaaacggc atgtattatt tttcataagt ccgtttttaa ggattaatgt tttcttttgc
      121 tttttatatg gtctggcttc agcgagaagt tccaacgcat atacaaggac gtctgatgtc
      181 aacgataaca acgattacca tggtggccag tccacttgga ttagcactgt ttggtgcgct
      241 ctttgattgg cagacaactc aaccacgtct gcgagacagt ctgatttttc ttgaaattca
      301 attggaaaat ttacactttc tttatacatt tctggctgct ttctttatac ccgtcaacta
      361 tacttcactt atcaataaga aagaaggata agtatggatt ttggaaatga aacggtcttg
      421 aaagcaactg gtataaccaa aaaatacggt gctgccaaag cattagataa agtatcgatc
      481 gaaatcaaac gtggaatgat ctatggtttg attggggaga atggagcggg taagtctacc
      541 tttatgagaa ctattatggg tttgatctcg attgatgaag gttcgatcga attatttgga
      601 acgacggatt tgcaggcagc aagacgacgt atgggtcaat cgattgaaac ccctgctctt
      661 tatccagaat taactgcaag agataaccta cgtatccaag cagcgaacgg cggtgtgtcg
      721 gatagagaaa tcgaagacct gctgaaaatg atgcgattgg aaaatacagg gaaaaagaaa
      781 gcaaagaatt tctctttagg tatgcgccag cgtttagcta ttgcaaatgc actgatcacg
      841 aatccagaat ttttgatttt ggatgaaccg acgaatggga tggatccagc agggatggca
      901 gaaatgcgag aaatcatcca acgtttggta aaagaacggg gcatcaccgt cttgctttct
      961 agccacttgc tagatgaatt gtcacagatc gccactcact acggtatttt gcatgaagga
     1021 cacttgatca aagaattatc gaaagaagaa ttggcacaag aatctcgtca atttataaaa
     1081 attgacactt cggcgacgga acaagcagta acggttctcg actctcttgg ttatagggat
     1141 tattttgtcc aatctagccg tgttatccag ctttttgaag gaattgacca agtagcagcc
     1201 atcaatcaag cattggtaga ggcaaaagtt cctgtggatg gtatccacct agttgggcaa
     1261 aaactggaag attatttctt acaattaact ggaggcaatc cacatgttta atctattaac
     1321 tgctgaaaaa ataaaagtat ggaagagccg taaaatgtgg gtctcactag gactgatggt
     1381 cgtacttccg cttttttatt cttggaacga atggtatatg catacaaaat acggcaatga
     1441 attaaaccaa gcaacggata cagtcatcaa cggtgcaaca gggattttaa tggtggaaaa
     1501 aatggctgca tggattcttc tggctttcgc agcatttgcg tgcttttata ttggggaaga
     1561 atttcaaaac ggtacgattc gcaatacgtt atctttagga cggaatcgaa tgacttatta
     1621 tgtgtcgaaa cttctcgtta cgcttttgat cacaacactt ggcgtggttt tgattacggg
     1681 gctagccatg attgcttaca cacttgcttt cggatttggt gaagttgagg gaatcaaaaa
     1741 ctatggaaat tatgttctaa aagtatttcc tgtattgctt ctgctgatat tagcgacatt
     1801 atctgttcca gtggctttga cattcatcac tagaagtacc agtgtcagtt tgcttctttc
     1861 ttttctttat atcatgggaa cggccttcgt tcccggtgtt ttcgcaaaaa tcaaaggctt
     1921 ggaatttcta acggagtggt ttacggaaac gtggctcatg tatacggact ttgcgcaaca
     1981 agcaacttat tcgcaagcac cgaaaatgat tctggtcagt ctagtaacaa tcgtggtatc
     2041 atcagctcta ggaatgttta ttttccaaaa atctgacatt aagtaggtgt tcctgctgga
     2101 aactattttg attatcgaag acgatgaagc tgttcattct ttactggagg aggttcttga
     2161 acagagatac aaacttcttg atgcttattc aggtacggaa ggaaaattat tgttaagcac
     2221 atatcctgtc gatttgatcc ttctagatct catgcttcca gggctttcag gtgaagcatt
     2281 gttagcagaa atccgacaga catcgaatgt tccgatcatc gttctttcag caaaaagcga
     2341 tcaaagagat aaagtttcct tgttggcagc aggagcggat gactatgtga cgaaaccatt
     2401 tgatatcgaa gaactgttgt tacgaatcgg catccaactt cgtcatcaga cttctgtcca
     2461 agtaaaagat ctgtcgcaac gcatctatta caaggaaatc ttactggaca aagagtcgag
     2521 ggatgtaaat gtgaatggtg tggctgtcca tttgacagga agagaattcg atctgctgaa
     2581 gcttttttta gaacatccga aaaaagtatt ttcgcgagca aatctttatg aaacagtttg
     2641 gcaagaacca tattttgata gtgagaaaac agtgaatatg catatttcca atcttcgagg
     2701 gaaactagca aaggcacatg ctgtgtatat tcatacggtg tggggagtgg ggtttagatt
     2761 tgattaagtg gctgttgctt ctcgttgtta tctttatggg aagttttatt ttcttcctgc
     2821 aaagggaaat caagagatta actaagaaaa tcgccactct ttctgaacga caagaatatg
     2881 gtggtcgtct ttcagtcgaa tttcgagaaa agaatctaat ggacctagta gatgcactga
     2941 atacatgggt ggatgaaaat gaacaaaaag gacaagcatt tcaagaaatg gaagaaaaca
     3001 tcagactatc gatcgtcggc ctttctcatg atctcagaac tcctttgact gctattaacg
     3061 gttatgtaca attactggaa caggtagagg atacggagaa acgggagaag tatttaacga
     3121 tcatcaaaca atctgttgca agattactca gtatgacgga tcagttttat gatcttgctc
     3181 gaatagagac gaagcaaaaa gaaatggatc tccatacgct ttctttttct aaagaggtgg
     3241 aagaagtatt tttctctttt tatgaacaat tttctgagaa aaatatcgag gtagcgttcg
     3301 ctgaacactc gataaataca actgtattag cagatccgct catgttgctg cgagtgctgc
     3361 aaaatatcat acagaattta ttgcgttatg cagatggaca agcaaaggtg acattttcaa
     3421 aagaggattc attcattcga ttaacagtgg ccaatcctat caaaccagga gcgcaaacct
     3481 ctgttgaaaa agtcttccag cgtttttata ccgaaaatac cagccgcaca aatacggaat
     3541 ctagcggaat cgggctttac ctgtcaaaaa aattggtgga aggaatggga ggagagatga
     3601 ccgctagatt agacgggggc atcttctcca tctccgtcaa gttgcggagg gtttaattgg
     3661 ctctaattta cctgaagtga atgacaaaaa attgtatctc gttttaaaga catcttcttt
     3721 tttggaaggt gtttttattt tggtgtgatt gatacgaatt aggtaacgaa gacagttatt
     3781 tttgtacccc cttttcaaat agcgcaacag tgctatacta aaactaacaa atcaaggaac
     3841 ggagagggat atgggccaaa gagaatttat cgttttaatt attattgcag ttgttatttt
     3901 attagttgtt ttagacattt tttcacgttt gaaagtaaag gaaacagttc gtagtaattg
     3961 gggaaaaatt ccttatcaac cgagatttga taaagaagaa agtttgaagg atgcgtggct
     4021 cacggagaaa aaattccgtt catgggatag tgaaatcgac gatcttactt ggtatgactt
     4081 ggatatgttc gaagtatttg aagggatcaa cagtacctat tctagtgtag gttcagaagc
     4141 gttgtatcag cgtttacgta gctttgattt tggagaagat tatcagttag aaaaactaat
     4201 tgctttttat caggaaaatc ctcaactgag agaaagaatc caatatcaat tcgctcgttt
     4261 aggaaaaaaa gatcataatt ttgcaaaaca atatctagcg gatggaaaaa gtaaaaagat
     4321 tggacgcgta ggtttattta tttttcttgg cttgcttccg ctggtgggtg taggtttgct
     4381 tcttttaggg caagtactag gaatctatct agcaatcggt ggcttagtat tcaatacggt
     4441 ctattatcta gtgaagaaac aaacactaga aacagagctg aatagcatga gttatttggt
     4501 ttcaacgatt tcagccgcca atcagttagc aaaaatgacg acaccacttc aaaaagagct
     4561 gaaacagcaa actaaaccat taagagacat ccctaaatta ggtttttcat ttcgaatgaa
     4621 gagcgggagt gaagcagaat tgatttttga atatgtcaac atcatgttca tgctgccatt
     4681 tatctcctat catttcgttc tcactaaatt agaaaagcat tcaactgaag ccatcaagct
     4741 gtgggaattg ctgggagaat tggaagttgc agcagccgtt ttgaattatc gcacctatat
     4801 gccgatcact tgccgaccag agtttactga gggcggtgtg acagcgaaag atatctatca
     4861 tccattgtta aaagaagcgg tagttaatcc agtcaactgg cagaagaata cattagtcac
     4921 gggttccaat gcatcaggaa aatctacgta tgtcaaaagt atagccatca gctgtattct
     4981 agcacagacg atccaaacag cgattgcaga acagtttacc ctgcaacctg gacatgtgtt
     5041 gacttccatg gcaatcgaag atgatttatt tgaaggcgac agttattttg tttcagaaat
     5101 caaatcgatc aaacgtttgc tggaccaagt ggcttccaaa gaacgctgct actgcttcgt
     5161 agatgagatt ttaaaaggaa caaatacgat cgaacggatt gcttcttccg cttcagtcgt
     5221 tcggtggctt gcggagtatc catctttagc atttgtcgcc acacatgata tcgaattgac
     5281 agagatttta aagaattatt gtgagaatgt ccactttgaa gaacaagtaa cagaaggaaa
     5341 aggaatcagt tttgatttca aattaaaaga aggaccttca aggacccgaa atgcgattgc
     5401 cttgctgaaa gttttgaatt atcctaaatc catagttgaa caggcccaaa aagaaagttt
     5461 gttgttcgat gaacagcgtc agtggtatcc gtttgattaa ctttatcaaa cgaagcatga
     5521 tgcgtgggtc caaaaagacg attatcaaaa gaagtacatt gatgccaaaa aagtggcaga
     5581 attcaatggg aacgagtctg ctgtccagat gatcttggaa gatttagcaa aagacgatac
     5641 aaaataaacc acgctgtctt ccttctagca gcttttatcg aaaaaactta gcatcagcga
     5701 cgagacgcac gttccatggc aaagttctcc gtaaaacaaa cagcgaaaac agaaattgaa
     5761 aataactatt tccattttat ttatttcggt taaaata
//
LOCUS       DACEMO010000159         5695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEMO010000159 DACEMO010000000
VERSION     DACEMO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.164"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            117..902
                     /locus_tag="IW728_002234"
     CDS             117..902
                     /locus_tag="IW728_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1025576.1"
                     /translation="MRESQGTPFEWKAGDSYVQLINIDKKDFLILKNLLGKLKYSSYI
                     KVVNYFVNTIFSEFSEKEKNEISEQIRKNMSYCSFSSADCKEKNEEYVVQLLTQIYFF
                     SRIFPYDTTHLTIRATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFW
                     MLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSK
                     YNLLEYDLIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(975..1622)
                     /locus_tag="IW728_002235"
     CDS             complement(975..1622)
                     /locus_tag="IW728_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1025577.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1630..2316)
                     /locus_tag="IW728_002236"
     CDS             complement(1630..2316)
                     /locus_tag="IW728_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025578.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2322..3395)
                     /locus_tag="IW728_002237"
     CDS             complement(2322..3395)
                     /locus_tag="IW728_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1025579.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3617..4291
                     /locus_tag="IW728_002238"
     CDS             3617..4291
                     /locus_tag="IW728_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1025580.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4296..5636
                     /locus_tag="IW728_002239"
     CDS             4296..5636
                     /locus_tag="IW728_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1025581.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
BASE COUNT         1841 a         1030 c         1009 g         1815 t
ORIGIN      
        1 tcgaaaaaaa gaagatcaaa gaaattatgg tcaagaataa ttgtcagatg aatttgtttc
       61 aggaagaaag cccatatgag caatctttct ttattatgta ttttgttgtg catctcatga
      121 gagaatccca aggaactcca tttgaatgga aagctggaga ctcttatgta cagttgataa
      181 atatcgataa aaaagacttc ttaatcttga aaaatctctt aggaaaattg aagtacagca
      241 gctatataaa agtagtgaac tattttgtca atacgatttt ttcagaattt tcggagaaag
      301 aaaaaaatga aatcagtgag cagatacgaa aaaatatgtc ctactgttcg ttttcatccg
      361 ctgattgcaa agaaaaaaac gaagaatatg ttgttcaatt attaacgcaa atttattttt
      421 tctcccgtat tttcccatac gatacgactc atttgacgat ccgtgcaact tactttgcac
      481 agcagatgca acaccgccag atacgtctat atgctggatt tcgtgcagaa cttcaatttt
      541 attccactct actgaatcag aatctgattt attatttatc tagctatatc ttttggatgc
      601 ttatcggtaa tcctgaaatt caccattata cttctgataa aaaaatactg attatcagcg
      661 acttaagtct tcgacatagt caatatattg aagaatatat cagtgatatt cttgctgtgc
      721 ataaaattca ctctgagaca actgctatta ctgaagatca gctgtcgaaa tacaatcttc
      781 tcgagtatga tctgatcgtt acgaatcaac ccattttgaa tgcccatgtt cctcatatat
      841 tgattgatga tagtgtgtca ttcgcaaatg aagaagaact catccgctta ttcgagttat
      901 gaataatcaa aaaacagcta tgtataaacc accctgataa aaatttcagg acaaatacac
      961 atagctgttt ctttttattg tattccttca gcaatccaac gagatttata gcgaaagtct
     1021 tttaacaact cttcttttgt ccaattattt gcaatgagat ccgtcaaagt gttcattacg
     1081 agggtcatgg aaaagtatag gaaactaact gcttcttccg acgattgaat ggagaaatga
     1141 ttgattctta ataattccag ccatttttcg ttcattggag aatcagccga tagttctcta
     1201 cgtttggttg tctgctgatc atttcgctgg gttaacaagt ataacctttg ccaatagata
     1261 ctttcgtgat ctaagtcgca gcaccactct aaatattttt cgatccccaa aaagaaatgt
     1321 tgtttgtttt cgctaatgaa ttttaagata tcttgatgga ctatgtgaga actgcttttg
     1381 atcgtataca aataagcatc ttccaaatct tgaaaatatt tataaaaagc accgcgcgac
     1441 atccccatct tgtcaacgat ttctgagact ttgacttggc tgatgtgctg tgagcaaaat
     1501 atctccagta ataggtcatc gattctctgt ttctttgcgt tcgataaatt gaaaaaagtt
     1561 tcttttggca tccacatctc tcctttatct ctgattatac gtggatagcc cttcattgtc
     1621 atgaaggatt cattccttct ttttctcttc ttctgtcaag tacccatctt ccattcgata
     1681 aacgcgatca ctccattgaa tcatacggac atcatgagta accataatag tcgctttttg
     1741 tttctcatga gcttctttaa ctagtaattt gacgacttca tatgcatgat ctgtatccaa
     1801 actagctgtt ggttcgtctg ctaagatcaa gcttggctca ttatataaag cgcgagcaat
     1861 tgccactctc tgtctttctc caccagaaag gtctttagga taactatttt taagtgactg
     1921 gatatctaaa gattgcagca aggaatcgat ttttgttgaa tcttcttttt ttgaagtaac
     1981 ttggtcaact agttcaaatt gttcctttac cgttaaaaaa ggaatcaaat tagagctttg
     2041 gagaataaat cctatttctt taaagcgtaa atctgctcgt tttttttctg gaagctcagt
     2101 tagtgagtga ccgttgatca ctatttctcc ggaagtcggt gtttgcaatc ctcctgaaat
     2161 ggtaaggaaa gtacttttac ctgaaccaga aggaccaatg atgctgacga attctccttc
     2221 ctttacataa aaatcaattg ctttcaaagc tgagaccttt gtatgaccag aaccaaatgt
     2281 tttggtaact tttttcatcg ttaatatttt tttcattttt attaacctcc tattgctgta
     2341 attgggtcta ctttggttat cgtacgtatc gaaaacaatc cgcctagcat tgcgacaatg
     2401 atcaaaatcc cactatatag caaccattgt gaccaaaaaa cagcaaaagg catcgcgctt
     2461 ggtaaaatca gactagtcaa ataagcaaac aaaagagcaa gcagaacgcc taccacccca
     2521 acaataaagg actgtgctac taaagattta gcgatgaaga aatttttgat cccttgtgct
     2581 ttcattactc caaaaatagc agtcttctga agtgtcatca cgtacatgaa aataccgacc
     2641 acggcagcaa ccactaagaa caaaaagtat atcatcgcat ttaaggtcat attttgggca
     2701 gaatagccag gaatactttc aatgaatgtc ccaatagtta ataactgtag acccttcgat
     2761 gctttttctt tcgaaatttt agctgaatcc tttattacga tcccattgat cggctgttct
     2821 tcttcagaag caaacggctg ttgtccatac ttcaaagcag tccacgtatc tagggacgca
     2881 tagatgacgg gagaaactgt gtaataagtt tcaggaaaga taccgacaat cgtcaaagga
     2941 tcgtcgtaac tgccaatttt gatcgtatct ccaattttat agcctgaatc agccagattt
     3001 tgagagatga tgatttcatt ctttgtttta aattcatgcc cttttgtgag ttttggtaat
     3061 aaaaatgctt ctttcgttgt gccgaaaact gtaacatttt ctttgttttt tcctttgatt
     3121 gctccgctgt acaatccgat tggtgctttc tctcctcctt caatgtattt cagatctcct
     3181 cttgttaatt gtgaagctgc aaatacttga tttgcatcct ccgacaaaac aatctcttgt
     3241 gcgtcccaat catcgattgc ttttttaaac tcttctgaaa gtccattcgc tagtcctgac
     3301 aacatgaata cgacatacgc aatcaagaac ataataccga cgatcaagcc atatcgtaat
     3361 ttagaatagc gaatttcttt gatagctaga aacatttgtt tttcctccct atgatagatt
     3421 gatagtgaca aaatgtcact tgttgaatag tgtaactcta aagtgacaat ttgtcacttg
     3481 tgttgttttt ttcttatcaa taaattgtct aagacatctt tcgataaaac cttaacatga
     3541 gtcataaaaa atttatttaa gccttattta tgctttccat gataaactaa agtgaccaaa
     3601 tcaatgaggt gggaaaatga ttaaactatt agtagtagaa gacgataaag tattatccaa
     3661 taatataaaa gaaatactcg ctgcaatcgg cgagataaca caagtttatg atggtagtga
     3721 aggactgttc gagttggaaa gtggagttta tgatcttgca atcttggatt tgatgatgcc
     3781 ggaaatgaat ggatatgaag tgttagcaaa aatacggaaa gaaaaaatac agactccagt
     3841 attgattttg actgcaaaag atagtttgga agataagatt gaaggatttg aacgaggtgc
     3901 agatgattat ctaacaaaac ctttctatcg agaagaactg atcatgcgcg tgaaagcatt
     3961 attgaaacgc tcgctaggtc tctttgatga aaatgttctg aagtataagg atatcagttg
     4021 tgatttaaaa agcaatgaag tcttgtataa agaacaagtt ttgcccatac aaggaaaaga
     4081 gtttgaattg ctcgtttatt ttttacaaaa taagggaatc attttgacaa aagagcagat
     4141 atttgaccgg atctggggat ttgattctga tacgacgatt acagttattg aagtttatat
     4201 gagcaactta cgaaaacatc tgcgaagtac tggattggat aaagaaatca aaaccttacg
     4261 taatgtcggt tatatactgc aaagggtgtg atgcaatgaa tggagatatt acacgaaagc
     4321 aacgtagtcg cttttttgcg acaaatgtag cagcatttgc ttttatcttt tttcttttgg
     4381 gattcattac tttacagata ttaaaccaat ctgcatacag agaaacagat ctgtcattag
     4441 agaaaataaa ttttgattct cgaacagtac aattagagat acaaagatat cagcaaggcg
     4501 atcccttttt aaacccgact caaacggaac aaaacagaac aaacatggaa cagccattaa
     4561 tggaaccatc aaatgaccgt tttaacactc aagtgatctt gtggtctaaa aatggtgaga
     4621 tattgaataa agcagctatt ggtgggcgtt tcaaccaaat caaagaatta aaaatggata
     4681 cgaaaaatct gaataccatc caaaatattg aattaaatga agacagtggc tcatctgccc
     4741 tctctttccg ttcgattacg aaaaaagcgg agacgaatca agatggcgtt gcctacatcc
     4801 aaattttaga aaatacaaat caaatcaaga attcattagc gacttttcgc acgattatga
     4861 ttttgtgtat gattgttttt tggattctct ctattggtat cagttattac ttgtctagtc
     4921 taagcatgcg ccctattatt ctttcttgga gaagacaaaa agaatttgtt gagaatgctt
     4981 ctcatgaatt gcgcacaccg ttaacgatta ttcaaaatag tttggaacat ctttttacca
     5041 agcctaatca tactattatt gaagaatccg aaagcatcgc tcaagcattg tcagagacga
     5101 ggcgcctcac tggattgact tcggatttgc tgacaatcgc tcggaatgat tctgatcagc
     5161 agctgctttc aaagcaaatg atcaatacac aagaatatat cgaacaactt gttaaaccat
     5221 ttcaagagat ggccatcata gatggaaaag agtttattct ccataacgat gcaacattag
     5281 aagtaccaat tgatgaaaaa aaaatccatc aagttttggt tattttgctg gataatgcat
     5341 taaaatatac gaatccagaa gataagatca ttttggattc atctgttaac cataaagaat
     5401 ggctgattca cgtaaagaat actggccctt ctatcagtga agaagatcaa gcaaggattt
     5461 ttgatcgatt ttatcgggaa gatcaatctc gggcgaaaga gactggcgga tacgggcttg
     5521 gcttggcaat tgcaaaacag gtaatcgaac agcatcatgg acagatttct gtatccaatt
     5581 atcttccaca aggagtcgat tttaaagtcc aattgccgtt gaagcctaaa aaataatgct
     5641 tatccctaaa aaagggctgt gacaaaaatc ttgtcacagc ccttttttgt tgcga
//
LOCUS       DACEMO010000160         5654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEMO010000160 DACEMO010000000
VERSION     DACEMO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.165"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(57..350)
                     /locus_tag="IW728_002240"
     CDS             complement(57..350)
                     /locus_tag="IW728_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1025582.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="IW728_002241"
     CDS             complement(378..1043)
                     /locus_tag="IW728_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1025583.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="IW728_002242"
     CDS             complement(1183..1815)
                     /locus_tag="IW728_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1025584.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="IW728_002243"
     CDS             complement(1822..2889)
                     /locus_tag="IW728_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1025585.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="IW728_002244"
     CDS             complement(2886..3617)
                     /locus_tag="IW728_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1025586.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IW728_002245"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IW728_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1025587.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IW728_002246"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IW728_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1025588.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         1607 a         1197 c          862 g         1988 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
     5641 gaatgatttt attt
//
LOCUS       DACEMO010000161         5586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEMO010000161 DACEMO010000000
VERSION     DACEMO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.166"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            160..2220
                     /locus_tag="IW728_002247"
     CDS             160..2220
                     /locus_tag="IW728_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025589.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2303..2872
                     /locus_tag="IW728_002248"
     CDS             2303..2872
                     /locus_tag="IW728_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1025590.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3047..3853)
                     /locus_tag="IW728_002249"
     CDS             complement(3047..3853)
                     /locus_tag="IW728_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025591.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3991..5505
                     /locus_tag="IW728_002250"
     CDS             3991..5505
                     /locus_tag="IW728_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1025592.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1987 a          968 c         1124 g         1507 t
ORIGIN      
        1 agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa aaatactctc
       61 ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat gaaaattact
      121 acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa aagacgttca
      181 aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc tgaaaaacac
      241 tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt ctatatgcat
      301 gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt ttctgagacc
      361 actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa acagctactt
      421 cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca ctactggtta
      481 attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct ttctatcttt
      541 tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga tattttgaat
      601 catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt atatgaaaaa
      661 gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa agtttcgata
      721 tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat gtccaaagta
      781 atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac tatttccttg
      841 tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat ccatgttcgt
      901 gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga gagttacgcc
      961 cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa taaagaagaa
     1021 atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt tgtattaact
     1081 tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta tgtagaagca
     1141 tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa taaagaactt
     1201 caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct acctgctgaa
     1261 ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg gaaagtcact
     1321 tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg gtcggagatc
     1381 cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg ggaaattacc
     1441 tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct tattgttcat
     1501 ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc cttaggtaaa
     1561 cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga ggatggttat
     1621 ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc gtattcactc
     1681 caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca gatagatcaa
     1741 aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat ggctcatgca
     1801 gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag aatatctaga
     1861 aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc acaagtttct
     1921 ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa agaatttgtt
     1981 gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa gaaaaaacgc
     2041 ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact tcctagtccg
     2101 ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt catgcgaggt
     2161 atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga ctggttctga
     2221 tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat ttggcataat
     2281 ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag aaatctggat
     2341 gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt ttgaatttcg
     2401 attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg ttggggaatt
     2461 tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt atcctgaaga
     2521 acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag caacctgtac
     2581 cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt tagcagtgaa
     2641 accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct tgcaagaatt
     2701 tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga gccatcaggc
     2761 gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag acggcacacc
     2821 taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat aacgaaatgg
     2881 tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat atcttacttt
     2941 tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa gaaggggcag
     3001 tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat cgtttgattt
     3061 ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga ctatagaggt
     3121 aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc ttcatagtat
     3181 ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt ttaccatctg
     3241 cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca gtgtttccgt
     3301 ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc atatcgaatt
     3361 gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact gtcatcgaaa
     3421 aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt gtatttttat
     3481 tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg aatgcatgta
     3541 gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct aaaactgttg
     3601 aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt atctttaata
     3661 tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa agcattgtat
     3721 ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg atacgtaata
     3781 aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta aaaaaggttt
     3841 tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa gaagcgtgtg
     3901 tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa aaatcactta
     3961 atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg tttattaaca
     4021 gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac agcgcagaca
     4081 agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca agtatataca
     4141 gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg tggtgcaggg
     4201 atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt ggaaaagatg
     4261 cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc agaaacaaaa
     4321 tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga aacgctaaaa
     4381 ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca ctctgctgat
     4441 gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat cactggaggg
     4501 atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg atacttagtg
     4561 gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa cgcagatgtg
     4621 acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt tcaaggagaa
     4681 gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt tggtgccagc
     4741 aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac aaatcaagaa
     4801 ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac cgttgatatg
     4861 gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat tggtgaggtt
     4921 gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt caatgaaatg
     4981 gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc agcttatttg
     5041 atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga ccaaaaagga
     5101 tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt accaaaagaa
     5161 acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa agatgctcaa
     5221 ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta tgcgatcaaa
     5281 atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa cactgaagta
     5341 ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt cacaggtggc
     5401 ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat ttttgggcga
     5461 caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag aacgtaaata
     5521 aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga ttgaacactg
     5581 tttagt
//
LOCUS       DACEMO010000162         5550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEMO010000162 DACEMO010000000
VERSION     DACEMO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.167"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(57..1010)
                     /gene="menA"
                     /locus_tag="IW728_002251"
     CDS             complement(57..1010)
                     /gene="menA"
                     /locus_tag="IW728_002251"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1025593.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1023..2126)
                     /locus_tag="IW728_002252"
     CDS             complement(1023..2126)
                     /locus_tag="IW728_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1025594.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2216..3151)
                     /locus_tag="IW728_002253"
     CDS             complement(2216..3151)
                     /locus_tag="IW728_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1025595.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3453..5387
                     /locus_tag="IW728_002254"
     CDS             3453..5387
                     /locus_tag="IW728_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1025596.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT         1609 a         1090 c         1107 g         1744 t
ORIGIN      
        1 tttggctgtt cttattactt gatgcagggc agttttttca caacctcttc atgctgttaa
       61 atggcttgca taatgattgt tagtaatagc cccaatacat aactggcatt gaataagacc
      121 atatttttga tggaatagct gaaactgata ggctggggta acgtttgctt gaattgatcc
      181 aaatgcttct tcacaattgg caaggtcaag aaggtgacca agatcggcca ttcatagata
      241 ccgctcaaca accccattaa aatcacgaaa tagcaagcat acatcaatag ctggaataag
      301 actgttcccg caggacgtcc gatataaaaa actagtgtat agcgatgatt ttcaatatca
      361 cggtcaagat cccgcaaatt attagccaac atgatattgg caatcgtaaa caccaagggt
      421 aaggaagcta atacgattgc tgctatagcc caaagatttc ctaccaaatc aaattgtcca
      481 gatgaaaagt tgactgtcag atcatagaca tctgaaccga atgtattgac ataaacgacc
      541 atggcaaaaa ttccaaggcc catagtcacc ccactgaaaa tttcccctaa aggcattcga
      601 gataaaggaa tcggtccgaa tgtatagaat actccaataa aacaacagat aaatcccatc
      661 aaaagtaaaa gccaacccgt attgatcgtt agaataacgc cgataacaaa aacaaatgca
      721 atcatagcta aaatcatgtt tcgaatcaat acaggagata gatttctgcg gccaatgaca
      781 ttttcttcat acttatatac ttcagaatga gccttttcat aatccatgta attattgata
      841 gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt gtttagccaa
      901 tgtatctcat gaaaaaaagc gatagagaat aataccccta ccgcaaaagg aaatacacta
      961 gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat ggttcgtact
     1021 ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga taatttgaag
     1081 gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa gatcgcttcc
     1141 gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc cattgaaaag
     1201 actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt gacacttgcg
     1261 atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta ttttttgcca
     1321 tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt ccgttcttta
     1381 atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac tgtccagtct
     1441 tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa gtcaacgatg
     1501 gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat gtaacctttg
     1561 gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt tttttctttg
     1621 tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat ttcttcttgg
     1681 gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc accaatcgcc
     1741 atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac taacgtgtag
     1801 acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc atcgatctca
     1861 gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc gaaggcaggt
     1921 ttcaatgctg attctttgcc ttcatcatac acacggatac gaacgtaagt ccctaacaag
     1981 aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact ttcagtggtt
     2041 gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc cagtgtgatt
     2101 aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa ggtgagacga
     2161 ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta tgtgattatt
     2221 tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca gctgcattca
     2281 ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct gtaacaactt
     2341 ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg tcgttcagag
     2401 tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca ttatattctg
     2461 tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat tcagttactt
     2521 tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag ttcttttctt
     2581 caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt gtatctgtgt
     2641 taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt tcagatgagt
     2701 gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag aattggtcat
     2761 tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg tttttgttgt
     2821 attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat tttgattcag
     2881 tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt gaataatttt
     2941 tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact tttgcagatg
     3001 attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa gaagagtttg
     3061 agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat gctaatacgg
     3121 caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc atttctagtc
     3181 cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata agtcaactat
     3241 ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt tcgtgaaaaa
     3301 acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaaaaga acagaacatc
     3361 tatataatat atggagattg tgtaataagt atcaaactaa aagaattatc atatattcat
     3421 tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt gtcgttgtag
     3481 gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg aaaaaagaag
     3541 atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc gagttgcatg
     3601 aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag catttgtttg
     3661 ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat aaagaacgaa
     3721 aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt ggaatgggcg
     3781 gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc ctttggtcat
     3841 ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa gctgcattgg
     3901 aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt tctggattta
     3961 ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc aaagattata
     4021 aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg atcttgaata
     4081 tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac aacgtggaat
     4141 tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg aaagacggac
     4201 gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact tctgacgctg
     4261 ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa tatatgcagg
     4321 caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt tatgaagaat
     4381 tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga cacacagccg
     4441 cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa ggaaactatc
     4501 aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt gataagatcc
     4561 atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa tacttctttg
     4621 atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc catatcaaag
     4681 atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta tggagtgtgc
     4741 cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa atcattggga
     4801 atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc actggaaaca
     4861 gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt gcttctgctg
     4921 ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa gcagcgactc
     4981 aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct gtgattgaga
     5041 agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat gatgtagcaa
     5101 tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca ttgattgctg
     5161 gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg ttctgcttat
     5221 caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca ttgatgaacg
     5281 gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa cgtacactag
     5341 gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata gataaagaac
     5401 gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac agcctccttt
     5461 ttatgtaaaa ttaaaggaat aagatgcccg agataagcct ccatgagcaa gacacaggtg
     5521 tcacaacctc tgttacaacc tcttgttata
//
LOCUS       DACEMO010000163         5491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEMO010000163 DACEMO010000000
VERSION     DACEMO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.168"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1279
                     /gene="gmhB"
                     /locus_tag="IW728_002255"
     CDS             <1..1279
                     /gene="gmhB"
                     /locus_tag="IW728_002255"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAQ1025597.1"
                     /translation="DIPKPMIPIDGIPVLERELCSLRDQGFNDIILTVSYLHEKIEEH
                     FGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFLLLNADAVFDVDFRQMIRFH
                     VENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKEDQRPEYYKNRVNAGLHVINP
                     KVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANTGRMYCYDSPEYVKDMGTPD
                     RYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVGFLRNAEEFELIDGVSEAIK
                     IINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETMLGHAGGFLNGIYYCPHHPHK
                     GYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFMVGDSESDVIAGKNAGCKTV
                     LITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            1314..2951
                     /locus_tag="IW728_002256"
     CDS             1314..2951
                     /locus_tag="IW728_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1025598.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            3053..3448
                     /locus_tag="IW728_002257"
     CDS             3053..3448
                     /locus_tag="IW728_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1025599.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            3466..4197
                     /locus_tag="IW728_002258"
     CDS             3466..4197
                     /locus_tag="IW728_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025600.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            4221..>5491
                     /locus_tag="IW728_002259"
     CDS             4221..>5491
                     /locus_tag="IW728_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1025601.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGK"
BASE COUNT         1811 a          699 c         1135 g         1846 t
ORIGIN      
        1 ggatattcct aagccaatga ttcctataga tggcattcca gttttagaac gagaattgtg
       61 ttcgcttaga gatcaaggct ttaatgatat tattttgact gtttcctatt tgcatgaaaa
      121 aatagaggag catttcggag atggttcaaa attaggaatt aaaatcaaat attttattga
      181 aaaaactcca cttggcaatg caggtgcatt gtattttttg ttggatgaac tgggtgatga
      241 accgttttta cttttgaatg ctgatgcagt atttgatgtt gatttcaggc agatgattag
      301 atttcatgtt gagaataacg cagatgttac tttgtttacg catcctaact cacatccgta
      361 tgatagtgga ttaattatat ccgatgacaa taagaaagtg cttcaatggt tagcgaagga
      421 agatcaaaga cctgaatatt ataaaaaccg agtaaatgca ggtcttcatg ttataaatcc
      481 gaaggtgttg agagaaaatc cattaatgga aattgtaaat aaatcaatag ttgaaggtgg
      541 ggggctacca tacaaaattg atttagatag aatgattctg aaaccattgg ctaacacagg
      601 gcgtatgtat tgctacgatt ctccagagta tgttaaagat atgggaacac cggaccgata
      661 tagagctgta attaaggact ttcaaaaggg aattattgtt ggtaaaaatc tatccaatcc
      721 acaaaaagca atctttttag atagagatgg aacgattaat aaatacgttg gttttttgag
      781 aaatgcagaa gaatttgagt taattgatgg agtatcggag gcaattaaga ttattaattc
      841 tctaggtttt ctgtgtattg ttataacaaa tcagcctgta attgcaagag gtgaagttac
      901 tgttgatgag cttaatagta ttcacaataa gcttgaaaca atgctagggc atgctggtgg
      961 atttttaaac ggtatttatt actgtccgca ccatccccat aaaggatatg caggagaggt
     1021 tatagaatta aaaattgatt gcaattgtcg caagccaaaa cctggactgt tattagaagc
     1081 atcaaaggat tttaacataa acctaagtca atcatttatg gttggagata gtgaaagtga
     1141 tgtaattgct ggaaaaaatg ctggttgtaa gacagtactg attacagaag acatcacctt
     1201 gcctgctaat tatggccaag actatagtgt ctgttcattg ttagaatttg ttaaaaatca
     1261 tttatgcact atgcaataat tggtttagtg aaacttgtaa aaggagtaat tagatgaagg
     1321 tattaattcc agcagcaaaa gttgttgaaa aagaattgca gagtatagga atgttaccag
     1381 caattatcta tccaattaat aaaaaaattg tctttgatta tttgtataat cagtataatg
     1441 caatctgcga ttcattggat attatctgtt atgaaaaagc ggataaggtt catagaagac
     1501 tgaaaaacta tcatgattcc agaattcata tatgtgacct tcctgaatta ggggatctag
     1561 ggcaaaccgt gtattttggg ttatcagatg aagatgagcc tgttctaatt aacttcgcag
     1621 atacaattgt tatggagagt atgtctgata tagaaatgga tacattctat tattctgaag
     1681 attttgtgtc gagtacttgg acatacttta aaattaatga aggaataatt actgatatcc
     1741 acgataaaga ggaagcttac gtatccgata aacgtgaaaa attatttatt ggcgtattta
     1801 tgttttcttc gtcaaaactt ctgaagagtt gcttaagaga ggcagttgag agtcggaaac
     1861 aggggggaat aaactcattt tatcgggctt tacagctata cagcgaacgt tgtccgctaa
     1921 aggccgtaag aacagaaaag tggtttgata ttggacacat cgataaatat tacaatacaa
     1981 agttggaagt taaagcacga gagtttaatc atatctctat agataaagat cgagggatat
     2041 tgaagaaaac tagcgatgac aaggataaat ttattggaga aataaagtgg tatttaaaac
     2101 ttcctgctga tattgaatat gtaagaccta gaatttttga ttattctaca tcttacgtag
     2161 ctccctatgt tgatatggaa tattatgcat atcatacggt tcatgagtta tacttatatg
     2221 gtgatttaaa atataaccag tggcatgatc tgtttagccg tataaagttt atctgtagcg
     2281 attttaagcg atacacctta aaagatgaaa agattagaga gtcattggaa gatatgtatt
     2341 tgcgaaaaac catgcagaga tttcaaaaat tgtctgaaga taaaagattt tcagcttttt
     2401 ttgagaaaca aattcaaatt aatggtcgta agtttatttc tttaaccggt gtaattgaac
     2461 ttctaaagaa tcttatccca agtatgctct atgatgtaaa tgaattttgt attatacatg
     2521 gagatttgtg ttttgctaat atgatggtag attccaattg ttcctttgtt aaggttattg
     2581 atcctagggg aaaatttgga aaatatgata tttatgggga cagcagatat gaattagcaa
     2641 aactatttca tagtttagat ggtaaatatg attatatcat caaggatcta tttactgtag
     2701 aatatgatac cgagtgtgca gtgatagatt atacaattaa tacgcgtgta agagattacg
     2761 atttatttga attgtttaag tcggtctttt cggacgagat taaggacgat tttgataaga
     2821 ttgtattgat agaagcgttg ctatttctta gtatgattcc attacacggt gaaagttttg
     2881 atcaccaaat agtgatgctt gctacaggta tggaacttct tgcaagagta agtaataaag
     2941 ttatactcta atataaattg tgccttaatg aataacggtt acaatactgt tttagcaatg
     3001 taggcattaa ggcacgatta tattaattaa tacaatagaa gggaagagaa aaatgtatag
     3061 tgaatattct tttgtgtttg atatcgatgg cactttgtgt ccgataaaga agaaggatga
     3121 gcgatatgag gatttggtgc cgtatgagaa tatggttgaa aaactgagat tctataagga
     3181 gaatggtgct aaaattacat tgttcacctc acgcaatgtt aattcctata aaggaaattt
     3241 gggaatgata aatgctaata ctgcaaaagt gctcttggcg tggttggata aatgggacat
     3301 tccgtatgat gaaatcctgt atggtaagcc ttggccgggg cataaaggct tctatgtaga
     3361 tgatcgaacg gttagacctg atgagttttt gagatgctct gtagatgaat taaatgaaat
     3421 ctgcaacagt tcaaaaaaaa gattctaaat aaggaggtta gttctatgga aaaaaagatg
     3481 acagttatca ttacgatggc aggtttggga agtcgttttc gaaaaattgg ttatgcggtt
     3541 cctaaatata tgattaatgc caaaggaaga actttatttg attgggccat ggatagtttg
     3601 gttggataca ataaaaatgt tgataagtat atttttgttg ttcgtcagga agacaacgct
     3661 tcagctttta taaaggagca ttgtgcttac tttggtattg aaaacgtaga agtgattgaa
     3721 atagactata tgacagatgg tcaagcaact acatgtatgt tggctattcc ttattgcaat
     3781 caagacaatg agattatggt ttacaatatt gatacatacg tggaatcaaa tgagatgaaa
     3841 tacgaagata tttgtggaga tggacatatt ccatgctttc atgctgatgg cgatcattgg
     3901 agttttgcaa agcttaacga tcgaaatgaa gtaatagaag tccgcgaaaa agttcgcatt
     3961 tctgataatt gtactcttgg tgcgtattat ttctcatcgg caaagctgta taaagcttta
     4021 tatgaagagt attatgcaga ctcctctaat atggagaaga atgaaaaata tatagcacca
     4081 ttatataact ttatgataaa aaaagggtta aaggtgacta ttagcatagt agatgcgaaa
     4141 aaggttcatg tgcttggtac tccagaagag ctcaatgtgt ttttgaatca agaataaata
     4201 gtgtttagga gaaaatagca gtgaccagta ataatctaat tgtaaaaaca ttaagcagat
     4261 ataagaatac tccacttcct gttaaggctg cattatggtt tatggtatgt ggttttttac
     4321 agagaggggt ttcaatgatt acaaccccaa tttttactag agttttgtct acagaacaat
     4381 acggtttata cagcacttat ctatcgtggg aatccttatt gctcatgatt acatcgctta
     4441 gtttgttcaa agccatgatg aatctttatg taaaatatga taatcaagaa catattcttt
     4501 cgacagtatc cggattgact ttagtactga ctattttttg gtttgttatt gctattttta
     4561 ttaaggatat attggcagaa attttaggat tgacaccagt tctagttgtc tgtatgttct
     4621 catattttat atttcaggca gaaatacaat gttggtcttt acacaaacga tatatatatg
     4681 attataagac tttattagta gctacccttg cagttactgt tggctcatct gtagttggct
     4741 gtttagcggt tctgtttata tctcctaccg ctgaggcgag agccttaatg aatgtcctag
     4801 tgactggtgt tgttggcgtg gctgtgtata tatcggtatt tagaagatgt aaggatttta
     4861 aggataatga agtgtggaat ttcgcagtag tattttgtgt atcgttgatg ccacactatt
     4921 tatctgaatt tattctgaat ggttctgata aaattatgat aaattactta tgtggttcgt
     4981 cagatgttgc tatttacagc gtggcatatt ctattggtgc tctaattaac cttgttacaa
     5041 attctgtcaa ttccactttt gcaccttatc agtatcaaaa aatcaagtca ggagaatatg
     5101 atttactatc aaaaagagct aatgaagttc ttggtttggt agcggctatg ctttttgcat
     5161 taatgctatt tagtaaagaa attgtgttga tatttggtgg gcagaaatat atcgacagtg
     5221 ttaatttaat tgtacctata tgtatcggta tttatttcaa ctatatgttt cagctgtttg
     5281 ctagagtaca ggaatacttt gaaaagaaac tcactgtcgt tattccatcg gttttgtgtg
     5341 caattatgaa tattgtacta aattatgttt ttataaagtt atatgggtat caggcggtag
     5401 catacacaac tgtattctca tatatgtttt tctgcgtcat ccattatatt ttttatagaa
     5461 aagtttgcaa agaactattg gatggaaaaa a
//
LOCUS       DACEMO010000164         5341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEMO010000164 DACEMO010000000
VERSION     DACEMO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.169"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(64..522)
                     /locus_tag="IW728_002260"
     CDS             complement(64..522)
                     /locus_tag="IW728_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1025602.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(535..1860)
                     /locus_tag="IW728_002261"
     CDS             complement(535..1860)
                     /locus_tag="IW728_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1025603.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1877..3289)
                     /locus_tag="IW728_002262"
     CDS             complement(1877..3289)
                     /locus_tag="IW728_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1025604.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3306..4070)
                     /locus_tag="IW728_002263"
     CDS             complement(3306..4070)
                     /locus_tag="IW728_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1025605.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4174..5049)
                     /locus_tag="IW728_002264"
     CDS             complement(4174..5049)
                     /locus_tag="IW728_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1025606.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1627 a         1124 c          783 g         1807 t
ORIGIN      
        1 tttcaaaaaa attttgtaaa ttcacccata aatatcaccc tatttaactt ttttcatcgt
       61 acactaaaat tcaaaacaac tatctttaat ctttacttct tggttttgtt ttaataaatt
      121 tccttcaaaa atgcctggag aaactacaat gatcaccgta tctttttgtg cttgctccaa
      181 cttcttcaaa tctataatag ctaacagtgt cccttctttt acctcctggt tactttcaac
      241 ccgtatctga aacggacttc cttcgagttc aactgtatct aaacccatat ggatcagtat
      301 ttttctgcca gattcacttt cgatcgttat ggcatgtcgg gtaggaaata tattatcgat
      361 tattccttta actggcgcat ggatatatcc atcatgattc gttatagcgt atccaactcc
      421 tatcattttt gaagaaaaga cctcgtcttt tactgctgtt aatggaataa tttgaccact
      481 agctacagct tttagaatgt ttcttgtttt aaagaaatgc actttttatc acctctactt
      541 taatgctggc caatattttt tgtttacttt gatcatttca tctagtattt tttttgcgac
      601 tctggcattt ggtacggttt gattcaatgt aaatgcttgt aaagcttttt gataagaacc
      661 ttcaaagtaa gcatctacta gtaacttctc tactgcaact tgtgcctcca tcaatccttt
      721 atgaaaatca ctgattggtt ttcttagtgt aattggctcc acaccagttg caccaacata
      781 tgccggtatt tcaacaactg catctgaccg tagattcgga atcgcccctt gattaggaac
      841 gattagcata aagcgacgac gatcatcatt caacaaggaa atagctatat cgacgatata
      901 ttgtccatgc tccccaaaat taaagttcaa tacttcttcc acaataccgc ggcgtatttt
      961 atcagccatt tctttggtat ttttctctcg tccatccatg accatattag cacgtgtata
     1021 ctctttgtca gtatgctcaa cgaccatatc aggatataag taatactcta agtaattatt
     1081 cgggatatta cttggaaaat ttttcgtgat gactgacatc atttgaaagg ttttttgcca
     1141 agttttatct cctatattaa aatcggcgac ttgcatttct tttgttatta gtttttcaat
     1201 aatctctggc ataatttctc gttgcaattc tttatcataa atcgatgtat accagccaaa
     1261 gtggtttaaa ccataatacg taacgatcca attcttacga tcataaccgt aattcactgc
     1321 aatcgtttct tcaattgaaa tagtcatatc acaggcatta atcattttta cattcggata
     1381 tttacgacga acagcttcag aaacgatcga ctctggattt gtataattca aaatccatgc
     1441 tttgggggca tagtcttgta tatgatcgac aatttccaat aaacctttga ttgagcgcat
     1501 cccataagca aatcctccga gtccacacgt ttcttgtcct actaatccat gtttaagtgg
     1561 aatcttttcg tccttttctc gcatctccaa tcctccaaca cgaatttgtg agaaaacaaa
     1621 atcgcatcct gtaaaagcaa gctttggatc atccgttgca cgaatgcgaa tatttgaaaa
     1681 accttttctt ttcagcataa atttaacaat caagtacata tccatatttc tttcttcatc
     1741 aatatcatat aaacgtattt cagataatgg caaccgtttt tcattgttca aaatggcttg
     1801 aacgattcct ggtgtatacg tactcccgcc tccagctata gtgataatct gttttttcat
     1861 catcatcttt tcctttctat tcttctaata atttattgaa ttctgccata acataagtca
     1921 cttctaagcc gatgataatt tgataatttt tttcagttgg tttaatgatc ccgcttatcc
     1981 ctaattgctt gattcgctgt tcctcgacca tggtgggatc cactacctct acccgtaggc
     2041 gtgtattaca actatagaca tccaggatat tggcttttcc tccaaacgct tgtagcattt
     2101 ttgttgccat atattcataa tttttacttg ctaaccctaa ctctgtttct tctaaggtta
     2161 cttcattgcc aaactctgcc ttagcttcac gtcctaatgt ctgaatgttt ctcttattga
     2221 tcaaataata aaaagtaacg taatacaagg caaaaaagaa aattccgatt ggaagaatca
     2281 aaatagcatt ggtagcgatt cgaaaattga tgagataatc aacaatacat gctccgaatg
     2341 taaaaccaat tcgtatattg aataagtaac agacaatccc tgctaaaccg gcaaaaagtg
     2401 catggatcac gtacaacata ggagaaagaa acatgaaact aaattcaaca ggctcagtga
     2461 tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat tcctttgacc atctctttat
     2521 tttctttata agctgcttta tagatagcta gacatatagc tggtatacca aacatcatta
     2581 ctacaaaaaa aactgataaa aacagtccag cctgaggatc tccgttgata aagcgtgtca
     2641 tctctcctct gaaaacttcc ttgctaggag atgtatagct tcctaaatcg taataaatat
     2701 acgtattcaa cacatggtgc agtccaaagg ggatcagcaa acgattcaaa aacataaata
     2761 tccccacacc taatgctccc atatcaacga gcagttttgc aaaattatca atcccaattt
     2821 ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat tgtttgtaga atcatcacca
     2881 tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac tactggtaat ttttgttctt
     2941 taaagcgatt atacgtccat gccgctacta aaccggaaat aattccacca aaaatcccca
     3001 tattcacttc tgggtccata atttctaagc cttttttcaa ggtagcaatc gctaaaaaac
     3061 cagtaagtgc aggtattcct ttgtccttat ttttgacaaa accgattgca actcctatag
     3121 caaataagat atccaagttt ccaaaaacgg catttccagc tgcaccaatt actgggatgt
     3181 tcagcatatc gtcagctgat aaacgcatga tcaatcccgc taatggcatc gctgcaactg
     3241 gaagtagcat cgctcttcca agcttctgca catttgattt aacattcatt tttattcctc
     3301 ctctattaaa tatcctcata tgttactaac tcaatcaaag gatcattttt cactttttga
     3361 gcaaatgctg aatgaattaa aaattcgact tggtgggtac gaggatacac aaaacttgaa
     3421 ccatttaaca attcctgatc caaatacgct gcgtgacaca tgacttcgat cgttttatct
     3481 tctctcaata tcatgagtaa acttgccaaa tattcttcca cactcgtatt gatttgtcgt
     3541 tttttttcat cagtttctcc tacgacatcg aaacaaggct caaaataatc gacatgacga
     3601 acttcatctt ttcgttcaaa gttgctccga accggcaaat catatttttt tgctaatgta
     3661 acaactacct cctgattttc atccaaagta tgagtatgat gatgactgtc taaatgagtt
     3721 ggtatgatcc ccgcatgata aattttttga atctgagcat tccattcctg atacagttga
     3781 tctttgtcaa tgttaaatgg atgctgataa taagataagg atctgaactc tccgctttcg
     3841 ataagagtat cgacagtttt gaggagtggt ttgccacacg tcaatgttaa atggactcct
     3901 acacctagcc taggcatttc ctttcttagt ttcacagcat gctcgaaacc cggcatattt
     3961 gccatcaatg tagttgatgt caaaattcct cgctgataag catccataat cccataattc
     4021 acaccatggc tatagccaaa atcatccgaa ttaaaaatca cttttcccat ttcattctcc
     4081 ttcttttttc tactaaaata ggcgcaacca aataatcata gtccatttac tatgtgtcat
     4141 ttggttgcgc ctatagatag ttacgccctt attttaatag attcttgcta gatgaattgc
     4201 caaatagatg atttcatttt cttgaatggc aagttgaaat tgctgttcga tgatatcttt
     4261 aatctttagt gctgtacaat acgctttgct atgtttaaat gtttcagtaa aaaaaccaat
     4321 attttctact ggcgtttctt cctgataaat caagcgttta accaggaatt tgatatgaac
     4381 cattaagcga tgataagtca gtgattcttt atcataaacg tttgaatagg atttttctag
     4441 atgggacaaa atcgtggtcg tcatttctac tatttttaag ctctttaaac tattaggctc
     4501 tcctagctca gcattaatga tatgcatagt gatgaatcct acttcatttg accctaactc
     4561 ggtttgatat cgatgattga ttcgctcgac cacttctact gctatatcat attcatttgg
     4621 ataaaggaat ctcatttctg gcaacataat tgctgtgaca tctctatctt ctctttttcg
     4681 ttgaatagca aaataaatat gatctgtcaa tgtaatatag atatttgaat ctaatggaga
     4741 ggagatccta gctgttatca gttcaatcac ttcttccgca aaatccataa attcgattgg
     4801 aatctgcgag actatctcac tgaatcgttt gatcttttct ttagaatcta atacaaataa
     4861 tttgtcagca tcctcaacat caaatggctt actcacttta gcagaaaagc caatcccttt
     4921 ccccatcgca attttttcta ttccatcgtc atcgatcaat acagcattat gattcagctt
     4981 tttcatcaca tacaccgtta attcacctcc tctatatgat aaaacaaaac aacttgcaga
     5041 ctccttcata catatttttg tctccaaaca atttttaaag acaaaaaacg acctaagaaa
     5101 gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc ctacataatt tacaaaaata
     5161 gtttaacaca taataaaagc gttatcaata tcttttttac tcgttttatg ataatacttg
     5221 tatcgaattg atttggaggt aattacataa aaacaaaaaa aatatagcaa ctctctataa
     5281 gaaagttgct atattgataa ttataccggc ggccggggtc gaaccggcac gtcctcaacg
     5341 g
//
LOCUS       DACEMO010000165         5291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEMO010000165 DACEMO010000000
VERSION     DACEMO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.170"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(128..373)
                     /locus_tag="IW728_002265"
     CDS             complement(128..373)
                     /locus_tag="IW728_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1025607.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            566..1366
                     /locus_tag="IW728_002266"
     CDS             566..1366
                     /locus_tag="IW728_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1025608.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1385..3253
                     /locus_tag="IW728_002267"
     CDS             1385..3253
                     /locus_tag="IW728_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1025609.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3246..3737
                     /locus_tag="IW728_002268"
     CDS             3246..3737
                     /locus_tag="IW728_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1025610.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3725..4279
                     /gene="srlA"
                     /locus_tag="IW728_002269"
     CDS             3725..4279
                     /gene="srlA"
                     /locus_tag="IW728_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1025611.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4297..>5291
                     /locus_tag="IW728_002270"
     CDS             4297..>5291
                     /locus_tag="IW728_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1025612.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1863 a          839 c         1077 g         1512 t
ORIGIN      
        1 ctaaaataga ttatctttat tcagtatatc acttaaacaa agaaaagcag atggtttaaa
       61 atgaaaaaac cttcccaata aagggaaggt tcatgaagat aataactttt ttaataatta
      121 tagctttcta atatctttca tcaacttttc tagtaatcat tgggaaaaat ttcaagcagt
      181 agatcgcaca aatacctacg acgatataaa tgatcttcgc aaaaatagtt gttgaaccac
      241 cagcaatcat agctactaaa tcaagttcga acaagcctac caacagccag ttcaaccctc
      301 ccacaatcaa taaagttaat gcaatagcat ctaagttttc attagaattc tcctttctta
      361 aatgataaaa caattatata tcaaaaagag aaacattgct tatataacgc tcaggaattt
      421 tagcaatcta tgcaaaaaga tgaaaatttt ttttgctctt ccattagtac acatttttta
      481 gctgaagctg taaatttcat gtcttaattt tttttagtga gcttttatac tggatttgta
      541 agaaaaaaga aggagaggat cacatatgtc ggattggttg gatattgcag gaaaagtagt
      601 aattgtaact ggaggttcct caggaattgg aagaagtatc gtcgaaaatt tgttgaagca
      661 gaatgcacaa gtagcgaatt ttgatgtaac tgaatgccgt atacagcatg aaaacctact
      721 atctttaaaa gtcgatgtgt cttctaaaac ggacatagaa gaagggatat ataaagtaat
      781 gaaacatttc aagacgatcg atggtctagt caataatgca ggaataaata ttccttctct
      841 tctaatagat agaaatcatc caaaaagcaa gtatgaatta tcagaacaag tttttgataa
      901 gatgatcgct gtcaatcaaa aaagtgtata tttgatgagc caagcagttg gaagaatctt
      961 ggtgcaaaag ggatcaggag tgatcgtgaa tctttcttct gaatcaggat tggaaggatc
     1021 agaaggacag agctgttatg ctgctacaaa agcagcgatg aacagtttta ctcgttcttg
     1081 ggcaaaagag ttaggaaaga ggaatgttcg tgtggtaggt gttgcaccag ggatactgga
     1141 agaaaccggt ttgagaacac aagaatatga agaagctctt tcttatacga gaggtataag
     1201 cgtcgaacag ctgagaaacg gttacagtcg aacaagtacg atccctctag gaaggagtgg
     1261 taagctgcaa gaagtggcgg atctagtctg ctattatctt tcagaaagat caagttatat
     1321 cactggagtc acgactaata tttcaggagg taaaacaaga ggataaagga taggggggac
     1381 gcacgtgacg ctagtaaaca gatggtacca attattgaaa ttactagtca ctcacaagaa
     1441 agtttcaatc caggaagtgc aaaaagaaat caaaaccagt aatcaaacca taaaaaaaga
     1501 tattggggag ttgaatgaag aaattgccgg tattgcaaaa atcatccaaa aaaacaaaca
     1561 ttttgaactt gaaatttatg attttgattt ttttgatgag atcatgcaag gaaaattgaa
     1621 aacagccagt gatttcaatt cggcatccaa aagaatcgct tatttactaa ataggctgat
     1681 cgaaacagag gatttcttac gtatggatga tttgtctgaa gaagtcggtg tcagcagagg
     1741 aacagtggtc aaggatctaa agacattaaa agagatgatc aatgattttg atgtaaagat
     1801 tacaggcaca ccaaataaag gactaagaat aataggagat gagctagaat tacgactact
     1861 tttattttat tttgtttctc cctacttttc agagacgaaa acagaacagt ttctagaaga
     1921 aaaacaagca atcaaacatt ttcagaagaa gacgaatgct agtaaacaag aaatcaccct
     1981 tttaacgaaa gttatcggta tttctttgaa tagaatctct agtaactatc tgttgaagaa
     2041 gcccatcaat cgttacagtg ggtgttttga atatatccaa gaattcaatg aactgatcaa
     2101 tcagttggaa gaaatctatg aactaacttt gagccaatat gaaaaagatt ttatcagttt
     2161 tcctttgaac atcatcaaca caagtataac gatgataagg aaagctaact ctgatttgcg
     2221 cttatatttc gaccccatga tgatgcgtat tcgacgatta gatctcattg atctggatga
     2281 gtccttctta tatcaagaga tgaaacagca cctctcttta ttgataaatc gggtaatttt
     2341 tcgttatcga ttgactgatt tattttatgg tgagatagaa aaaaagtatc ctttttctta
     2401 ccaaatcgct agcgaatgca tacaagcatt agaagctatc ttgcaacata agatctctaa
     2461 agtagagatc agctacctaa cgttgtattt ggagttagca tttcgatatc aaaaagagca
     2521 tttttcctct aaaaaagtag ccattgttca tacgacagga aaaggaacag cattaatgat
     2581 ccaaagacaa atcaaaagga tattggggag ggatatacaa ataacacatg tttcagaaga
     2641 acaatatgaa aaagtagatc taaacaaata tttcgctgtt tttacaacga ttccgttaaa
     2701 aaatgtaaat gaagagacac cggtgatcca tttgactaat ctttttgatg actcttggtt
     2761 aagggcacaa tggcaaaaag caaaaaaaaa gcgattagcc gggtttcatt ctactgtttt
     2821 tttctatcaa atatggaaag aaaaaaagac gtatcaagaa tatttaaaca aattagtcgc
     2881 tcctttgatc agaagctatt atgtggatga agaatttgaa aaaaggatct tgaaaagaga
     2941 atatcagcaa agcacatttg ttaacaaaga atttgctttt cctcacacaa ccaaccagct
     3001 atctcaacac attgttttat cagtagggct aataactgat caaggtttag cagaaacagg
     3061 aggaaatgga gttatttttt tatttgccat acctgaagaa atgacggctg aagttgagaa
     3121 tgaattattt gagctatgcg atatcctatt tgaactcata aaaatgggaa tagatgagtt
     3181 tattgaatta gcaccgacac aagaatcatt tttaaaacta atcaaaagta aaggagagag
     3241 acttcatgaa tgatatgtat ctattcatag ttttcgcagc ttcagcttat gtgctgcaag
     3301 cagttatcag ttggaagcaa ctaaagcatt tcaatcaaac atatagcgaa ctgcgaagaa
     3361 aaggaaaagt agcgattggt aaacgacctg ggaaaatccg ttcaggcacg attgttatgc
     3421 tagctataga cgatcatgat caagttttag aagtcagaaa aatgcaaggg gttacatctt
     3481 tggcaaaatt tcattcatta cctgtctatg cgggtgaaga tatccattac atcgatcgtt
     3541 accatccctc agttcgagcg gaagatcgtt tgacacaagc agctttagaa aatgccagag
     3601 aagtatactt acgaatagag gcaggaaatg aaaaaagtga acaaccttca ttgcctttta
     3661 ttgctatgct caataatttt cggatatgga aaagtacgat caaagaaaaa aggagtgaca
     3721 aacgatggat atcataacaa aatttgctga gggatttatg aggctgttcc aaacaggtgc
     3781 agaaacattc ataagctgga tgacaggaat cgtaccagtt gtcttgatgc tgatggttgc
     3841 aatgaatacg ctgattgctt ttttaggtga agaccgagta acgaaagtag ccaaattatc
     3901 agcgaaaaat ccgcttactc gttaccttgt gttaccgttc ttatctgctt ttatgttggg
     3961 aaatcctatg tcatttacaa tggctcgttt ccttccagaa tactacaaac caagctatta
     4021 tgctgctcaa gctcaatttt gtcatacgag caatggtgta ttcccccata ttaatcctgg
     4081 agaattgttt gtatggatgg ggattgctca agggatcgaa accttagggc taaattcgat
     4141 ggagctggct atccgatact tgcttgtggg actgctgatg aactttattg gtggttggat
     4201 aactgatttt actactggat ttgtatgcag acagcaagga atcgttctta gcaaaaaagt
     4261 agagctttca gttgattaga gaaaggagag ttttaaatgt atggtgatat taagatcgta
     4321 agaggaaacg gcgggtatgg aggtcctttg attattaggc cgacaaaaga taaacacaag
     4381 ttcatctatg tcacaggtgg aggtgagagg ccaatgatcg tagataaaat agaagaactg
     4441 acaggtatgg agtctatcaa tgggttcaaa acatcaatac ctgatagtga aatcgcgctt
     4501 gcaatcgttg actgtggagg gactcttaga tgtggaattt atcctaaaaa agggatacca
     4561 accatcaata tcgttcccac aggaaaaagt ggcccattag cacaatatat cactgaagat
     4621 atttatgttt cagcagtagg gttagaacaa atacagccac taatggaaac tgatgataac
     4681 aaagaaacag taaatactgc atctccagat aaaaagtaca aatatgatac ttctaaaaag
     4741 ataaccgagc aaaaagcaga gcagcaaaac tggatagcaa aaattggaat gggcgcggga
     4801 aaagtagttg ctacattcaa tcaatcagca agagaagcag tagatacgat gatccggaca
     4861 gtgatccctt ttatggcttt cgtttccctt ttgattggtg tgatacaagg ttctgggatc
     4921 ggtaatgtat ttgctaacat tatggcacct ttagcaggaa atgcagttgg attaacaatc
     4981 attggcttta tttgttcact gccattttta tctcctttgc ttggtccagg ggcagttatt
     5041 agccaagtga ttggaacatt gatcggtgta gagattggga aaggaaatat tcctccccaa
     5101 ttagcattac cggcactatt tgctatcaat actcaaaatg catgtgattt tattccagtc
     5161 ggtctaggtt tggaagaagc aaaagctgag acagtagaag taggtgtacc atcagttctt
     5221 tattcaagat ttcttaacgg catcccacga gttatcattg cttgggtagc aagttttgga
     5281 ttatatagct g
//
LOCUS       DACEMO010000166         5288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEMO010000166 DACEMO010000000
VERSION     DACEMO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.171"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..176)
                     /locus_tag="IW728_002271"
     CDS             complement(<1..176)
                     /locus_tag="IW728_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1025613.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYV"
     gene            complement(170..571)
                     /locus_tag="IW728_002272"
     CDS             complement(170..571)
                     /locus_tag="IW728_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1025614.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(756..2564)
                     /gene="uidA"
                     /locus_tag="IW728_002273"
     CDS             complement(756..2564)
                     /gene="uidA"
                     /locus_tag="IW728_002273"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ1025615.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KLITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWKERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLQKRWSSIPNFSYKK"
     gene            complement(2587..4353)
                     /locus_tag="IW728_002274"
     CDS             complement(2587..4353)
                     /locus_tag="IW728_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ1025616.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IISDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIEPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(4366..5160)
                     /locus_tag="IW728_002275"
     CDS             complement(4366..5160)
                     /locus_tag="IW728_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1025617.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYYQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGEYVDIEQKFIDTFWKNLTNK"
BASE COUNT         1663 a         1045 c          649 g         1931 t
ORIGIN      
        1 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
       61 taactatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
      121 aagataatgg atagtggaac taataggtac tgccgctttt tggatttatc taccataggt
      181 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
      241 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
      301 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
      361 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
      421 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcatttcgtt
      481 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
      541 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
      601 ctaatacaac aatcagagag cccctttttt tcgaaaaaac tagaaaaaaa cataaaaata
      661 tttatcattc tttaaacaga aaatctttct ataaaaaaca aaaaaacgat taggctaggc
      721 taatcgtttt ttgaacattc ttaaaaaaat ccaaattatt ttttataact gaaattgggt
      781 attgatgacc atcgtttctg tagatagtga gcaatcattt taggttttct atctctagta
      841 aagattcctt tcttatttcc ttgcactctt aaaatccctt gactcgttgc aaaatctgca
      901 aaattccaca cttgttcacc aacaaaattt tcacatgtat ctattacttc gtgatttgct
      961 tcgtaataag ccacttgata ctcttctgta aacattgttg gtactgtatc atgaaatcca
     1021 gctactgtat ctgctccata ttctgtaaac attattggcg tatcaggaca tctttctttc
     1081 catccatcta gctctttttt caacagatat tttgcatgtg ccagatcccc tcccgaaaca
     1141 taccaaccat aataacgatt taaacataaa acatcgacaa tatctcctac cttacaaata
     1201 tctggcgttg attttagata agtaactatt gttacaggtc ttttctgcgg atcaagttct
     1261 ttagttaatt caactaaagg ttcaaaatat tctttagcac cttcggaatc cgaatctggt
     1321 tcatttgcaa tagaccacat tacaacactt gggtggttct tgtcgcgttc tatcaattcc
     1381 ttgatcaccg ttttatgaga attttctgtt cgtatctctt tccacgtatt atgtttttcc
     1441 acattatcat ctaataaagt ggccatgaaa tttaaatgta accctactgc aggtacttca
     1501 tcaataacta caataccttc tcgatctgct aatcgcataa tttcttctga gtaaggataa
     1561 tgtgatgttc taaaagaatt agcacctatc catttcatta aattaaaatc tttaatattt
     1621 acagcctcat tcaaaccacg tccattaaca tacgaatcct catgcttacc aaatccttta
     1681 aaataaaaag gtttattatt aatcaaaaat ttgccattgg taacctgaac actgcgaata
     1741 ccaaattctt ctgtataggt atcgatcact ttattatcat caattaaact aattttcata
     1801 ctatataaat aagcatctag aggttcccaa aaaattggat tcttaagctg aatcattcct
     1861 ttctttcctt cagcttcagc tacaagttga gcctcttcgt catatatcgc tattaagatt
     1921 tttccggtcc attctgtatc gatattgaat tctacacttg catccaaatc agttattctt
     1981 gtattaatag taatatcact tatatagtct ttcggagttg tatataactt aacaggtcta
     2041 tgaatacctg cataattgaa gaaatcaaaa ttaactaagt tttccttttt tatatcccca
     2101 ttttcctgta ctgtttcctt cataatcccc acaggtaaag tactatcgtc aataatattg
     2161 tttacagcaa ctgtaattcg attattcccg tcttttacaa accgattaat ttcagactca
     2221 aatggtgtaa acccaccttt gtgttgagta atcaactttc cattcaaaaa aacttttgct
     2281 tcatgtgttg cagatccaaa acgaagaact aatctttctt ctaataatct tttaggaaga
     2341 gtcacttctt tttcatacca cacccatcca acatgatttc taatgttctc tgtttcaaat
     2401 aaatcattat aagaagaggg aactgaaatc ggataactct ctttttcatt tattggttct
     2461 aaagcaagtt cttcctgtaa gtcatcattt gtatctaatt taaatctcca gagaccgctt
     2521 aagtcaatta cttgcctcgt ctctgtcata atgggataca acatataaac cactcctatc
     2581 taaatattaa ataaatgtat cctttaaacc acaataagca tctattggac ttactccctt
     2641 aaattcagaa agacccatta gtttgtccac caatatttct aaagtttctt gttttccatc
     2701 ataagtatta atataagtct ttacttgagg tacatcagcc aaatgaaacg gacactgtac
     2761 agatacaaaa attgttggaa tttcatgaat gtaaaacggt atatccggag ttcctttact
     2821 tgctggccag acaattcgtt gaactgtgcc tggattaaca ttagctatat taatgatcaa
     2881 gtcatatttt ttagttaaat ttttaattgg tctcttagca gcatatattc cagctatatt
     2941 tttaattttt tcttcttgcg ataatccttt caactcttct tcaggtgaaa tgtatttttc
     3001 aacttcaaat ccttttgctg acaaaatctg tttcaataaa tcaatggctt gaggtctatt
     3061 tccagcaact acttcaccaa acccaccttt taccccatgt acatctacta gtaaaatcct
     3121 tttatatttt tctggttcaa taggccaaat atcttgttta tctttaacta atgtaattgc
     3181 ttggtcagcc acctctttaa aaattgattt attttcaatt aaaccaattt tttccagagc
     3241 ttcttgctta ggtaataaaa tttgttcttt tgcttttttg tgtaatccca atttagcttt
     3301 taaccctaaa attcttttta aggcatctaa taaccgatca tcagagataa ttcctttacg
     3361 ataaccttcc atcatataat taaagtcttc atctggatca ttaaaaaata gaaatagatc
     3421 acatccagct gcaatagaag tgggcaataa ttctgatcgt ttcattgccc cagtcattcc
     3481 taacatatga ctagcatcag taactactaa tccattaaat ccgagttttc ctctaagtaa
     3541 gtcagtgatt aattcttttg ataaagttgc aggaagtaaa tcttcatctt tcagatcagg
     3601 attaaaatgt tttgaataag ctggcatatg gatatgacct gccataatcg aaggtaatcc
     3661 agcttctatt aaacctgagt atacttttcc aaaagaatta tcccattctt ccgtacctaa
     3721 actattaata ctaaaagata gatgctgatc tcgttcgtca ataccgtcac cagggaaatg
     3781 tttagctgca ggcaaaattc cactctcctg aatacctttc atataagcta aactaagttc
     3841 taaaacttta tcagggtttt gtccaaaaga tcgactagaa atgataggat ttctccaatt
     3901 tctattaata tctacaatag gagcaaaact ccagttacat ccaatagccg atgcttcaat
     3961 cccagaaact ctccctaatt cataagcata tttcgcatca tttgttgcac caatttttac
     4021 ttctacacca acttcagtac catctgtaca agctccgtta ccacctgctt ccgtattagc
     4081 agcaattaat aaaggaattt tgctttctgt ttgtaggata tagttttgat catatacctc
     4141 atcagcagtt cctgggttat atcgtacagc tccaatatgg tactgattga ccatattact
     4201 caagtaattt ttatcacgac tagatcccat attaacaaac aattgaccta ttttttcttc
     4261 caaagtcata gaatcaatcg tctcttcaat ccaagaaata gcatcttcat ctaaattata
     4321 tggttttttt cgtaagtcta cttttatagt cattttcttt ctcctctact tattagtaag
     4381 atttttccaa aaagtatcta taaatttttg ttcaatatct acatactctc ccgataaaca
     4441 tttaaataaa acatcagttc ttaagttttc ccatgattct ttttctttat taaccaaaat
     4501 aggataaaag aaaactttat ttttatttgc agcttctaga tcaccaggag aatcaccaat
     4561 cattaatatc ttatccaaat cacaaccatt ttcaattaca cttcgaataa tgtcttcttt
     4621 ttttccttta tcttgacaac acagttcatc aactaatgat aataattcat gtctttccca
     4681 ttcttcttga acagcttcct tattagctga gcttacaata attatcttcc catatttttt
     4741 cagagctttt atagtatctc tagcacttaa aaagggttta tctaatcctt tagcttgact
     4801 aatctgttga ttgacttctt ccgaccattt taaagctttt aataaatcct ctgactgata
     4861 atattctatt tccctcttca aagatggatt agacaattct tttgttagag atacccagtg
     4921 ctttaatcga ttaatggatg ttacatcatt tttttctaat gttttaacta aacctaaaaa
     4981 tctgtttatt ccacgagtct ctgaatataa attaatatta ttccaatcct gtaggaattt
     5041 ttctctatga tgaacatcaa aaatctctgc agcaattggt ccgaaaaaga gattgtgttt
     5101 ataagtcatc gtatccataa cacagccatc tgaatcaata caaaatatcc aattattcat
     5161 atttctctcc tatttatatt tttgttttaa aagctcgcaa ttttctaaga ctttatcttt
     5221 agataatgga taaaggaaaa ataaaattaa tcctacaata aaaaaacaaa ttgatggaat
     5281 taatgttg
//
LOCUS       DACEMO010000167         5222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEMO010000167 DACEMO010000000
VERSION     DACEMO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.172"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1128..1793)
                     /locus_tag="IW728_002276"
     CDS             complement(1128..1793)
                     /locus_tag="IW728_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1025618.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1919..3955
                     /locus_tag="IW728_002277"
     CDS             1919..3955
                     /locus_tag="IW728_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1025619.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3959..4597
                     /locus_tag="IW728_002278"
     CDS             3959..4597
                     /locus_tag="IW728_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025620.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4625..5053
                     /locus_tag="IW728_002279"
     CDS             4625..5053
                     /locus_tag="IW728_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025621.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1743 a          789 c          952 g         1738 t
ORIGIN      
        1 tgttttagtt taaactagtg tgtaatttaa aaatctatat ggaagggtct gtggggtaat
       61 acttaaataa aggctaggaa cctgataaca tcaaggttcc tagccttttt ataataaaat
      121 gaagcgaaaa taatttcttt taaagagccg agattatcag ctatataaga gtgaatttga
      181 tgcaaaaaac agagccgaaa cagtcttggt gttcgtagct ctgcttatat gtttcgaatt
      241 taagacaaag agttggcaaa agatgttgat aaggaagata agataactgt gttatataaa
      301 ctaggtaact aactgtaaca agctaatttt aagagattct tattagaaga cgtttaggaa
      361 tataactaag aaaatgatta tacaggtaat tgtcatttca ataatgtttt ctaattttcg
      421 cgtctttgtt ttaagaaaaa ggccagaatc agttggaaaa aagttcgtac gatagtgaag
      481 aggggcaaaa tttcctacta atattcccac taacaagaga taaaacggcc aggttggtgt
      541 gattcctcta taaaaaaatg cccatgctac cgaaatccca tagattaaca actgaacaat
      601 ttttacaatg aaaaaattgt tctgccatta ctcaaagtct cttcctcctt gtatcgttta
      661 tttgtttcat tttatcatta tgataggcgc ttgaacaatc cattttgtat tttattgagt
      721 actcttggat aaactaccag cgattatcat atttatttat gttactgtaa gccatcatat
      781 aaaagtaaat gagcagatca tagaaatata aaaaagaact aaaattgatc taaattatgt
      841 gcagcttgac tacgtaccaa tctatattac ttactagatt ggtcgttaat ctattcggat
      901 cgaatgagca aaatattttt gtgaatgtcc atccttgatt atgattccaa aaagaatcat
      961 tgggaggctg tggcgtaaat attatactat aagccagaaa tacctatcat tctgaacttt
     1021 tttatttagg aactttcatc tcaaggcttt ttcaattaat ttgtacccct gttttttgga
     1081 taagatcatg tgcattgatt ttttctatct ctacgttcat ctcctactta atgcctatct
     1141 ggctcctgag ggcttctttt taaggcgtcg tcatagtctt ggctgaggat ttcttggtaa
     1201 tctgagggaa tggcttccca agtgagcagg cgaacgttca tgacgtgttt tcctttgtgt
     1261 ttgattacag catggttttt ctctttttct acctctccag cgtgaaaaga cagccatgta
     1321 ccatggccaa agatatcatt acatacccaa cgttcgatgg aattgtccac gtatcctaca
     1381 gggagtgtgg tatagccgat ttgtggtttc aaaaaaggat tgaatcgatc gatcgtttca
     1441 tttttgtatt gataattgat aagaaaaaga tagaccaaac aaaagacgag cagaaagaaa
     1501 gatagtgtct ttttgagtga gaagggttgt tttgatgaat aagatttctt gttaacaaga
     1561 ggttcatctg gcaatgttgg ttctattgat tcggtagctg attcaataga aggaaaatca
     1621 atcggcagac ctaaagtatg atgtaaaagt gacagcattt caaggtctgg cattgtacga
     1681 gaattttccc aactagaaat cgttttatcg gaaacaccaa ttttttcgcc taattctttt
     1741 tgcgtaagct tacaatttct gcggtaagtt cgtatttttt ctccggtgtt catcttcttc
     1801 tcctttcgat tctacagtat atagatttat tctacatgac ctatacactt gatagatacg
     1861 tttttttgat gaaaaacggt atgattttat caaagaatca tttaagtaag ggagagtggt
     1921 gataatcaaa aaaaagttca cagttttact cacaatcata gtgaccggta tcatgagtgg
     1981 ttttgccttg gttagtataa ataaacaggt actctatcgg acaaatgaat ttttccatca
     2041 aacagatact tatactccga ttcgattgcc acaacatttt attgggcaag aagaatatga
     2101 gcagctaatc atggtatttg aaaatgtctc atcagaaact gagctttctt atatgaaaaa
     2161 aacagtaaat gaatccaatg aagtagatac atcttggaac ttcaagatac agccaaagct
     2221 tgaaatgaca ttgtattatc aaggggatgt caaatcttct cacgagaagg taccctattc
     2281 taatacctcc tttcgcgtaa cgtccattga agaatcgatc aaaataaaag attttgaagg
     2341 tgaatttttc ttgatgacga gagaaaaaga agcctatgaa cggttcattt cactattcac
     2401 tgatcgattc aatcaagcat ttcacactga ttatgccact aatatttttg aagactttga
     2461 aaatagctat tcgtattcgg ttatttcaaa tacagacgta tataattatc gagtgtattt
     2521 aactattgga ttgattcttt tattcctttt gttatcatcc tttttcttat tgatgaataa
     2581 agaaatcagt ttattgttga ataatgactt ttcattacat caagtattgt ggtgtttatt
     2641 ggggaaaaag aaaatcatta ttacattcat aattgcagga atcattggtg gttttatttt
     2701 tatgtattat aaggagcaat ttctttattt gttccagatg tacgttttct tgttcagcgg
     2761 aatatatggt atctctttga tttctctttt attagttgaa aaaatcgctt cgttaagaga
     2821 agcgttcaac tatatccaat acattctttt tacatttgga ttgttgtttt ctcttctttt
     2881 gaccgttgcc aatgtcgaat tagcaactat cgttttttct tcttcacagt tagctgtttc
     2941 ccatgaacca ccaaaagaaa tcgctggtga ggactatcat gtattttatc cagtaaccat
     3001 cgggaaaaac cacgtggatt ttatttattc caatcgtttt gcatcagcag cagatcagga
     3061 attatatgaa actttgaata aaaacggaag tatattagtc aatgtatcag attacttgaa
     3121 tgaagaaaat gaacagtttg gcagagggat aaaaataaat ctgaattatc ttaagaaatt
     3181 tcctttgatt gatgtctctg gaaggttgat ccaaataacc gaaaaagaaa cgcaccctgt
     3241 tatattgatc cctgaacgat atagacatac agatttaaaa aatgacctgg cgcaaataac
     3301 agaatattat caagaaatca gtgaaaaaga accgaaattt ctatttataa aaaacaatca
     3361 acccatttat acatttattc cttctatccc ctggatcgaa cattatccgg ttgttgaaat
     3421 actgacattg aaaaacagta tgtatttgga aagaaatatt ctcagcggag aaatctatcc
     3481 accgttaaag atcaagatag gctctttgtc gaaagaaagg ttatttgagc tgattgaaga
     3541 aagtcagtta cgtgataact tgcagttatc atttccgtat actcagactg aagagatagc
     3601 tgtcaaagtg ttaagcagat catttcatta cttgatacag atatttttac tcactttctt
     3661 ttctttcttc ttgttgagct atgtcatgtt atgcatatat tttgtctaca attgtcggaa
     3721 aatcgctatc tttcgttcaa gtggttattc gctctttgaa acgtataaag atttcttcat
     3781 gatgaacctc atcaaatggg gaacgacaag tgtaatcttc ctttttctta tagaaagaga
     3841 gcctaaatat ctttttaata tttttttctt tagcttcatt gattttattc tttcagttgt
     3901 ttttattttg tctacagaaa agaaaagtca gttattgctg atgaatggag gttaacaatt
     3961 ggagccgttg atagtgataa aaggtgcgaa aaaaatcatt cgaaacaaaa cgattcttca
     4021 tttggatcgt ttcactgcgg atgctgggga tcttgtgact gttgttggag aaagtggagc
     4081 aggtaaaagt acgttattga atatattagg attgaatgat cagttcagtt caggacaatt
     4141 ctatttgttc gatcaagaga cgaaacaatt aactgggaga gagaagcgct tgataaaaag
     4201 aaataccata tcttttcttt ttcaagattt tgggttgatt gaatatgaaa caatcgattt
     4261 taatttagat attggattga agttttcgaa tttaaatgtt tcagataagc gaacagcaaa
     4321 gtatgaggca ttgaaaaaag taaatataaa caagaagcta agtacgccta tctacacatt
     4381 atctggtggt gagaagcaaa gaatcgcctg tgcgagagtc ttattgaaga atagcaaaat
     4441 cattttagcc gatgaaccga ctgggtcatt ggataaaaga aataaggata aagtagttga
     4501 aatcttatcg caacaagctt cccaaggtac gttagttatc gtagtcacgc atgatccata
     4561 tctgaccaaa ataggaaaca agaagatcat gctttaaaaa tcaaaaagga aaggatgtcg
     4621 ttttatgaaa aaagcagtta ttatttgtgc actagggata ggttttgttg gaggaaacgc
     4681 tatgtcatct gaagtggaag caaattcagt tcacggtgaa atctattaca aatctttaaa
     4741 gccaggagag attcattttg atgctaaagg taatattatt aagtcaaatt taccagtaga
     4801 ccgggaaatt tctagaacag caattaatca tagctctgga agatgggttt attatagtga
     4861 tacaacttgt acgagaaaaa caggtcattc taaccactat agtcgtgtct ataatcgtca
     4921 cggtgcaaaa gcgaaagttg gaaactctat tcgatcagca aatgcaacaa aaggagtatg
     4981 ggcctatgca agtgcgacag gaaatagaac tgacacattc tcatgttggt acaatccaac
     5041 cggttggtat taagtatcca aaagtttata taaaatagaa aaaacatata atgaaacagc
     5101 gttaaaagtt atttttggta gttttaatat attcattttt agttgtataa aaatatatca
     5161 taataaaaac aacaaaaaaa tgtctggaaa atcgaacata aaagtgtctg atttgaatat
     5221 cc
//
LOCUS       DACEMO010000168         5020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEMO010000168 DACEMO010000000
VERSION     DACEMO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.173"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..246)
                     /locus_tag="IW728_002280"
     CDS             complement(<1..246)
                     /locus_tag="IW728_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1025622.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSA"
     gene            581..1015
                     /locus_tag="IW728_002281"
     CDS             581..1015
                     /locus_tag="IW728_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1025623.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1038..1358
                     /locus_tag="IW728_002282"
     CDS             1038..1358
                     /locus_tag="IW728_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025624.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1499..1690
                     /locus_tag="IW728_002283"
     CDS             1499..1690
                     /locus_tag="IW728_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025625.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1761..2018)
                     /locus_tag="IW728_002284"
     CDS             complement(1761..2018)
                     /locus_tag="IW728_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1025626.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2176..2439
                     /locus_tag="IW728_002285"
     CDS             2176..2439
                     /locus_tag="IW728_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025627.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2463..2651
                     /locus_tag="IW728_002286"
     CDS             2463..2651
                     /locus_tag="IW728_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025628.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2734..2919)
                     /locus_tag="IW728_002287"
     CDS             complement(2734..2919)
                     /locus_tag="IW728_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025629.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3134..3505
                     /locus_tag="IW728_002288"
     CDS             3134..3505
                     /locus_tag="IW728_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1025630.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3655..3727)
                     /locus_tag="IW728_002289"
     tRNA            complement(3655..3727)
                     /locus_tag="IW728_002289"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3692..3694),aa:Gln,seq:ctg)
     gene            complement(3773..4201)
                     /locus_tag="IW728_002290"
     CDS             complement(3773..4201)
                     /locus_tag="IW728_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025631.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4261..5019)
                     /locus_tag="IW728_002291"
     CDS             complement(4261..5019)
                     /locus_tag="IW728_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1025632.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1748 a          783 c          990 g         1499 t
ORIGIN      
        1 tgcagagcct tctgttggga ttgttaattg ttgtcctgag tagatcaggt taatatcaga
       61 aattgaattt gattctgcaa tggcattgat caaggagtta tcaccaacgt atttttgaga
      121 aatcgttgat agagtatcac ctgattgaac ggtataagct tcgtccgcat gagcagtcgt
      181 tcccatgaag aatgcagcgc cggcagccaa agttgttcca aaaagtaaag ttttaagtga
      241 tgtcatgaaa aatctccttt attcgttaaa aagttttatt tgttgtgttc aacgaggaat
      301 actttaacac gtgaaaatat ttcataggta aaagaagcat gacaatttat gacaaatcca
      361 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
      421 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      481 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      541 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      601 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      661 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      721 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      781 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      841 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      901 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      961 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
     1021 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
     1081 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
     1141 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
     1201 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
     1261 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
     1321 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1381 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1441 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1501 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1561 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1621 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1681 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1741 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1801 agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc
     1861 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
     1921 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
     1981 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
     2041 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
     2101 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
     2161 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
     2221 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
     2281 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
     2341 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
     2401 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
     2461 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
     2521 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
     2581 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
     2641 gtttatttta gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt
     2701 cctcctttga ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt
     2761 tttgacactg tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc
     2821 taattgttct ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat
     2881 catttccttg actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat
     2941 caacatgatt ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata
     3001 tcaaataacg atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa
     3061 aatctccaac ttgggacgga tggaatttct ttaactgacc catatactga atatataaca
     3121 aggaggtaga aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat
     3181 tttgctattt ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact
     3241 tgatcagcta aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga
     3301 ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa
     3361 agaacgaacg gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat
     3421 tagcaataaa ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga
     3481 gaaagcatta gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat
     3541 tattcataat ttaattatgt aaactaaata caagtagtaa aaataataca ataatccccc
     3601 tgcaaaaaga ttgttctttt acagggggtt cattttttca gcaagcacat caaattggga
     3661 tggcagggtt cgaacctaca attgcttgat tcagagtcaa gtgccttacc agtttggcta
     3721 catcccattt attcaacaaa ataatcatag cactttccta aaaaaacgca agttattttt
     3781 cttgatcgga tagttgttgc tttactcgta aaaggcggcg tactagtttc ttttctaatc
     3841 cagcaggaag ggcaatcagt tctgctactg acaagacggt gtccaataac tgatattctt
     3901 tgacaggttc attcataata tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag
     3961 tatactctgt tcgagcaatt agaaatttca ataaatcggt ggctttctcg tactcttgat
     4021 ctgcgtaata aacaagagat tgacgataca atttgatttc cagttcattg agggtagcag
     4081 gtctgcgacg ctcgttccac tcattaatag agactttacc ttgaacattt cccttgcagt
     4141 cacggacaaa aataatcgga atttcatttg gatccatttt tttatgtgtg aatttttcca
     4201 ttatctacgc tcccttcaaa ggtttggtat cttgagtata agggattttt gtttgaatta
     4261 ctagcttttc tccaaagtta gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc
     4321 gttgatcggt actgctcgat tttttacacc atagggatcg ttgacataaa tattttcttt
     4381 gtcatatcca gtgactacac aagcatgaac aagaggagtg actcttactt ctccattatt
     4441 tgtggtccaa gtcataaaat cagtttcatc tgggacttca aaattgatag tcacagaagt
     4501 ccagacagga tttcctttag atagtgcttt tttgatatca tcgagttgag catcggtttt
     4561 tggaaaagtg tgataatccg tttggacgat ttgctgagca agggaggcaa ctgggggaac
     4621 atagacgacc atagcccagt ctccgccact gatatcacca acaaatcctt cattaggatc
     4681 gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc aattgctcag ctaactggtt
     4741 tttagttgtt tcataaccat aaaaagataa aagcatagaa agcgaagtga cttcacagcc
     4801 attttctaaa gaaggagaat caaactggct ctctaatgga acatctagta aaacttctgc
     4861 tttctctttt gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg
     4921 ttctggagaa tggatatgta ggtaataact tgtaccgctt aataaaagag cagctgtgcc
     4981 ccctagaaac aatagttttt tgaacgtttt tttcttcatt
//
LOCUS       DACEMO010000169         4820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEMO010000169 DACEMO010000000
VERSION     DACEMO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.174"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1967
                     /locus_tag="IW728_002292"
     CDS             <1..1967
                     /locus_tag="IW728_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1025633.1"
                     /translation="RKAKRGAAIVGTAMVGCSVAAPLVQPVPVQADETPVQFGARINT
                     AAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSALSQAPYYNLFGIKGSYQGQ
                     TVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRNTSFGNGYYYAGTWKSNTK
                     SYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPASGNAGGGATTGGNNTGSAG
                     STGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITMDQLIQWNNIQNNFIYPGQ
                     KLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKYTVQSGDSVWKISNDHGIT
                     MAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTGNTSGSSSSSSSSSNTASG
                     TKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQQLTVSNSGSTNSTSGNTG
                     SSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGITMDQLIEWNNIKNNFIYPG
                     QQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGNTMYTVKAGESVWSVANKH
                     HITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVTGSKTYTVKSGESVWSVAD
                     SHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            2394..4808
                     /locus_tag="IW728_002293"
     CDS             2394..4808
                     /locus_tag="IW728_002293"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1025634.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT         1641 a          912 c         1039 g         1228 t
ORIGIN      
        1 ttcgaaaagc aaaacgaggg gcagcaatcg tgggaactgc gatggtaggc tgttctgtcg
       61 ctgccccatt agtacagccg gttcctgttc aagcggatga aacacctgtt cagttcggcg
      121 caagaatcaa tacagcggca tttatcacag aaattgctac ttatgctcaa ccaatcgctc
      181 aagccaacga tctatatgca tcagtgatga tcgcacaagc agttgtagaa agcggttggg
      241 gcagtagtgc gttatctcaa gcaccttatt ataatctttt tgggataaaa ggcagctatc
      301 aaggacaaac cgtctacatg gatacgcttg aatttttaaa taatcaatgg gtaaataaaa
      361 aggagccttt ccgacaatat ccttcttttg ctgaatcttt caatgacaat gcctatgtac
      421 ttagaaatac ttcatttggc aacggttatt actatgccgg tacttggaag agcaacacga
      481 aatcctatac agatgcgaca gcttgtttga cagggcgtta tgcgactgat cctggttacg
      541 caggcaaatt aaataatatc atcgcaactt acggattgac ccaatatgat acaccagcta
      601 gcggaaacgc aggcggtgga gcgactacag gtggcaataa tactggaagt gcaggtagta
      661 ctggcggttc aagcaattct ggtaatacaa actcaggttc agcaacttcg accacttata
      721 ctgtccaatc tggtgattct gtttggggaa tctcaaataa attcggtatc acaatggatc
      781 aattgatcca atggaacaac atccaaaaca atttcattta tccaggacaa aaactgacaa
      841 tcaaaggtag ccaggaaaat ggctcttcga cgaataattc cggtaacaat accaactctt
      901 caggaaatgc tggtacatca aacggaggac aaacaactgg tgcgaaatac actgttcaat
      961 ctggtgattc tgtttggaag atctcaaatg atcacggaat tacgatggct caattgattg
     1021 aatggaacaa tatcaagaac aatttcgttt atccaggaca acagctaatc gtatcaaatg
     1081 gcggttcttc taatgctgga gcaaatactg gctcaaatac tgggaataca agtggctcta
     1141 gttcgtccag ctcaagttct tctaatacag cttcaggaac gaaatataca gttaaagccg
     1201 gagactctgt ttggagcgtc tcaaataaat acggaattac gatggctcag ctaatccaat
     1261 ggaacaatat ccaaaataac tttatctacc caggacaaca actgactgtt tcaaacagtg
     1321 gatcaacaaa cagcacgtca ggtaatacag gttcgtcaac caatacaaat aacagcagtt
     1381 cagttaatac tggcagcagt tcgtctcaag gcacttatac cgtgaaagca ggtgaatctg
     1441 tttggagcgt ggcaaataaa aacggcatca cgatggatca gttaatcgaa tggaataata
     1501 tcaaaaacaa cttcatttat ccaggacagc aattgatcgt taaaggtgga acttcggcaa
     1561 atacaaatac aggttcaact acttctgcag cgaaaccaaa tacaccgaat acgtctgcta
     1621 cgacaagtac atcatccact ggtaatacga tgtataccgt gaaagcgggc gagtcagttt
     1681 ggagcgtagc gaacaaacat catatcacga tggatcagtt aatcgaatgg aataatatca
     1741 aaaataattt catctatcca ggacagcaag tgattgtgaa gaaaggttca tctcaatcaa
     1801 caaaccaaca atcagttact ggttcgaaaa cctatactgt gaaatctgga gaatctgttt
     1861 ggagtgttgc tgacagtcat ggaattacaa tggctcaact aatcgaatgg aacaatatta
     1921 agaacaactt tatttatcca ggtcaaacgt tgattgtagc taaataaaat agctgttcga
     1981 agaaataacg attgaattcc acaaaaatat ttgttactat aaggctactt aaatatgaca
     2041 acacttgaaa aggaagtagt aaaaagactg aagtttcaga gagcgcatgg ctggtgagaa
     2101 tgtgcacgga tgcttttgaa ctcgcctttg agttgccctt gctgaatacc cgagtaagca
     2161 agcggcggtg acggccgtta caacgttcga ggccttttca ctaaggtaaa attaggtggt
     2221 accgcgtaga ttacgtccta taaaaagagc aatcttttta taggattttt ttatttcttt
     2281 tgtgaagttt gagaaaacaa agcaaaagga aataaaaata aacagattct gccggttttt
     2341 tcgttatatt tataatagaa acaagtgttt tcaatagaac aggaggacag caaatgagtt
     2401 acaatcataa agaaattgag aaaaaatggc aaaaatactg ggcaaaaacg aatactttca
     2461 acacccatga tgatcctgaa aagccaaaat tttacgcact ggatatgttt ccctatccat
     2521 ccggtcaagg gctacatgta gggcatcctg aaggttatac ggcaacggat atcctttcac
     2581 gtgtgaaaag aagtcaagga tacaacgtgc tgcatccaat ggggtgggat gcattcggtc
     2641 tgcctgctga acaatatgcg ctagatacag gaaacgatcc agctgagttt acccaaaaaa
     2701 atatcgaaac attccgacgc caaatcaatt ctttaggatt tagttatgac tggaatcgcg
     2761 aagtgaatac aactgatccg gagtattata aatggacaca atggatcttt acgaagcttt
     2821 atgaaaaagg tctagcttat gaagcagaag tagcggtaaa ctgggtacct gaattaggaa
     2881 cagttatttc aaatgaagaa gtgatcgatg ggaaaagtga acgtggtggc tatgatgtag
     2941 tcagaaaacc aatgcgccaa tggatgctga aaatcactgc ttatgctgat cgcttattgg
     3001 atgatctaga attagttgat tggccagaaa gcattaaaga aatgcaaaga aattggatcg
     3061 gtcgttcagt aggagcaaat attgaattta aagtggctgg tacggataaa tcctataccg
     3121 tatttacgac acgaccagat actctattcg gtgcaactta ctcggttctt gcacctgaac
     3181 tagatcttgt tagagaaatt acgacccctg aacaaaaagc tgctgtagaa gcttatatcg
     3241 aagagacagc taaaaaatca gatctaaaga gaacggatct tgcaaaagaa aaaacaggag
     3301 tatttactgg tgcttatgca atcaatccag ttaatggcaa ggaaatccca atctggatcg
     3361 ccgattatgt attatcttca tacggcacag gagccatcat ggctgttcca gcacatgatg
     3421 aacgtgactt tgaattcgct caaactttcg gactagaaat tcttccagtt atcgaaggtg
     3481 gagatgttga aaaagctgct tacacagaag atggtacgca catcaactct gaatttctag
     3541 acggcatgaa caaacaagaa gcaatcgata aaatgaatga atggctagaa gaaaatggcg
     3601 tagggaaaaa agaagtcagc tatcgcttac gtgactggct attctcccgt caacgttact
     3661 ggggtgaacc aatcccaatc atccattggg aggacggaac agtcacagct gtcccagaag
     3721 aagagctacc attacggttg cctaagacaa aaaacatcaa gccaagcggt actggagaat
     3781 ctccattagc aaatatcgag gagtgggtca atgtcgttga tcctgtaaca ggtaaaaaag
     3841 gccgtcgcga aacgaataca atgccacaat gggcaggaag ctcatggtat tacctgcgtt
     3901 atatcgaccc tcataacaaa aaagaactag cagattatga aaagttgaag cgttggctac
     3961 cagttgacat ctacattggt ggagcagaac atgcggtact tcatttacta tatgcacgat
     4021 tctggcataa attcttatac gatatcggtg ttgttcctac aaaagaacca ttccaaaaac
     4081 tatacaatca aggaatgatc ttaggcgaaa acaacgaaaa aatgtcaaaa tctcgtggta
     4141 atgtagtcaa tcctgatgat gtggtcgaaa cttacggtgc agatacactg cgcatgtacg
     4201 aaatgttcat ggggccatta gatgcttcga ttgcttggag cgaaaatggc ttagaaggaa
     4261 gccgtaaatt cttggatcgt gtatggcgtt tgatcgttga tgagaacaat aaaatgcgtg
     4321 accgtatcac tacattgaat gatggcaagc ttgataaagt gtatcatcaa acagtgaaaa
     4381 aagtgacaga agactatgaa aatcttcatt tcaatacagc aatctcccaa ttgatggtct
     4441 ttatcaatga agcctataaa gtggacgctc tgccttatga atatatcgaa ggatttgttc
     4501 aactactcgc accgattgct cctcacatgg gtgaagaact atgggcaatc cttggaaatg
     4561 atggtggtat ttcttacgca ccatggccaa catacgatga agcagcactt gttgaagacg
     4621 aagtagaagt tgttttccaa gtaaacggta aagttcgtgc gaaaagcaat gttccccgag
     4681 acttaggaaa agatgagcta gaaaaagcag cattagccaa tgaaatagtg caagaatata
     4741 ttgaaggcaa aacagtcaga aaagttattg cggtaccaaa caagttagtc aatatcgttg
     4801 ctaattaatt ggctctttgt
//
LOCUS       DACEMO010000170         4805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEMO010000170 DACEMO010000000
VERSION     DACEMO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.175"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..556)
                     /locus_tag="IW728_002294"
     CDS             complement(<1..556)
                     /locus_tag="IW728_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1025635.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLI"
     gene            complement(773..1252)
                     /locus_tag="IW728_002295"
     CDS             complement(773..1252)
                     /locus_tag="IW728_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1025636.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            1611..2393
                     /gene="rpsB"
                     /locus_tag="IW728_002296"
     CDS             1611..2393
                     /gene="rpsB"
                     /locus_tag="IW728_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1025637.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            2492..3373
                     /locus_tag="IW728_002297"
     CDS             2492..3373
                     /locus_tag="IW728_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1025638.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            3509..4231
                     /locus_tag="IW728_002298"
     CDS             3509..4231
                     /locus_tag="IW728_002298"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1025639.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            4234..4791
                     /gene="frr"
                     /locus_tag="IW728_002299"
     CDS             4234..4791
                     /gene="frr"
                     /locus_tag="IW728_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1025640.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1685 a          862 c         1005 g         1253 t
ORIGIN      
        1 aaatcaaaaa atcaatccct agttcggcta cacaaatcgg tatcccacca aagcttgaca
       61 tcgcgtccac tataaacaaa agatctcttt cttgtgtgac agcagcaatc tcttccaaat
      121 tattcaaaat accggaagta gtctcgctat ggaccattgc tagtgcggag atagacggat
      181 tctcttcaat cagctgtgta atcacatgag cttggaggat ttgatgttcc gatacctcat
      241 aaacggtata gttcagtttc agacattcgc acatgttgat cattcgttgt ctatatgcgc
      301 cgtttgaaca aatcagtaca gtatcttctg gcctgatcac agaacttaat accgattcta
      361 ccccgaagga gctgctacct tgcattaaga ccgttgtgta tattccttcc gatacttgag
      421 caagatccaa taattttttt cttatccatt gtgtaatttc tttgtattct tgatcccaag
      481 tacaatgatc gatcagcata gcatttttca ctgtttcaga agtcgtcaat ggtccagaag
      541 ttaataattt atacatatga tttcctttca tttctttttt gaaatacaca aagagcaaga
      601 agctgctctt gttaacatcc aatataaatg atgattattg tgattcaatc aaggtgttga
      661 aaaatttgaa taaaaaatga tgtatttttg taaagagtgt aaaggtttat tcaataagga
      721 aaatattgaa aataaaaaaa cacgcattcc atattaggaa tacgtgctct gattaaaaaa
      781 gtgatacttc agttggatgg tgaagaaatt cttctactcg ttttgcgtcc tcctcatttt
      841 ccgagatgac catgatcgta tcgaatccag caattgtact gacaatagag ggaggtttga
      901 tctcatctaa aacagatgca aataaatgag cattgtctgg caaggtattg acgatcgtca
      961 aaaagtggac tcgttcgact ttcaatacaa tgtcttcaat catttgaacc agccgatcct
     1021 cttcctcagt cctagtctca ggagttcctt tttcgcgaaa caatacgaag cgggaaacgc
     1081 cattttcatc aggtgccttc acgattttta gatcacggat atctcgagaa atcgttgcct
     1141 gcgtggcggt aacgccataa tgcccaagtt tgtttaatag ttcttcttgc gtacggatcg
     1201 ggaattcttc aatgacttgt ttaatcagca aatgtctttg agctttgcgc attttctttc
     1261 tcctttacga aagatttcac atgtatcata acaaaaaaaa gaaggattgc ccaataatat
     1321 tcgtctttta tacctaaaat tattttatgg aaaaaggata ataaagaaac aaagagaaat
     1381 ctactgtttt tcttgaattc tttttatctt tcaggtaaac tattgaatgt tgagcagaaa
     1441 agtatatttg ctcttgcgat acggatcttt tgtctgtata atagctgagg gtgctttatg
     1501 catcagcaaa tccacattct tattggattg tcggtaggtg cttgaaaaag tttgccggca
     1561 aggtagaaga taagaagagg aaaaataaaa ccaaatcgga ggaaacaaac atggcagtaa
     1621 tttctatgaa acaattacta gaagccggcg tacactttgg tcaccaaact cgtcgctgga
     1681 atccaaaaat gaagaaatat atcttcacag aaagaaacgg aatctacatc attgacttgc
     1741 aaaaaacagt taaattagta gatgcagctt atgattacat gaagagcgtt gctgaagaag
     1801 gcggcgtagc attattcgta ggtacaaaaa aacaagcaca agaagcaatc aaagaagaag
     1861 caactcgtgc aggacaatac ttcgttaacc atcgttggtt aggcggaaca ttgacaaact
     1921 gggatactat ccaaaaacgt atcagccgtt tgaaacaaat caatgcaatg gaagaagatg
     1981 gaacttttga agttctacct aaaaaagaag ttgctggttt gaacaaagaa cgtgaacgtc
     2041 ttgaaaaatt cttaggcggt atcgctgata tgccaagaat cccagatgta atgtacatcg
     2101 ttgacccacg taaagaacgt attgctgttc aagaagcaca caaattaaat atcccaatcg
     2161 tagctatggt tgatacaaac tgcgatccag atgaaatcga cgtagtaatc ccttcaaacg
     2221 acgatgcaat tcgtgccgtt aaattgatta cttcaaaaat ggctgatgct ttcatcgaag
     2281 gaaaccaagg tgaagatcaa gtagttgaag aagatttcac agaagaaaac acagcaacat
     2341 caatcgaaga aatcgtagat gttgttgaag gcgacaattc ttcagcagaa taattttgaa
     2401 tgatggagct gtttcaaagg ctgggcgaca agcctcactc tcctttgaag cagctttttt
     2461 taaaaagaaa aacacacagg aggagttaaa catggcagac gttacagcta aaatggtaaa
     2521 agaactacgc gaaatgactg gcgtaggaat gatggatgcg aaaaaagcat tagttgaagt
     2581 agaaggcgac atggaaaaag cagttgatct tttacgtgaa aaaggaatgg caaaggctgc
     2641 gaagaaaaat gaccgcattg cagctgaagg attggcttca gttgctgtga aaggtaacac
     2701 tgcagcaatc gttgaagtaa actcagaaac agactttgtt tctaaaaacg aaatgttcca
     2761 agatttagta aaagaaattg cagaacttgt tgctgaaaac aaaccagcag acatggaagc
     2821 tgcaatgaaa atcaaaacag ataaaggtac aattgaatct gacttgatcg aagcaactca
     2881 agttatcgga gaaaaaatca gcttccgtcg ctttgaagtt gtagaaaaag acgacaatgc
     2941 tgcatttggt ggatacttgc atatgggcgg acgtattgcc gtattgactg ttctagatgg
     3001 aacaactgac gaaacagttg caagagacgt agctatgcac gtagcagcta tcaatcctcg
     3061 ttatgtaaac gaatcacaaa ttccagaagc tgaattagaa catgaaaaaa ctgttcttac
     3121 agaacaagca ttgaacgaag gcaaaccagc gaatatcgtt gaaaaaatgg tagaaggacg
     3181 cttgaagaaa ttcaaagcag aaatcgcttt ggtggaccaa ccattcgtta aagatcctga
     3241 tatgactgtt gaaaaatacg ttgcatcaaa aggcgcaact gttaaaacat tcgtacgttt
     3301 cgaagttggc gaaggtatcg aaaaacgcga agataacttt gtagaagaag ttatgagcca
     3361 agtgaaaaaa taattctgtt gaataacaga cgggaacgca cggtttgtgc gctccctttt
     3421 tttaagcaaa acaagaaaaa ccagtcttag agtgtcgaaa tcatctaagt atggtaaaat
     3481 ggcaatagaa aatattggag gaatgaacat ggtcacaccg aaatatcagc gtgtagtctt
     3541 aaaattgagt ggagaagctt tagctgggga cgcaggattt ggaatcaaac caccagtaat
     3601 caaagaaatc atccaagaaa tcaaagaagt tcacgagctg ggcgttgaaa tggcaatcgt
     3661 tgtgggcggc ggaaatatct ggcgcggaca aatcggagca caaatgggca tggaacgtgc
     3721 tcaagctgat tacatgggta tgttagctac agttatgaat gccttagctt tacaggatac
     3781 gttggaaaac gtgggtgtac cgacacgcgt gcaaacttcg attgaaatgc gtcaaatcgc
     3841 agagccatat attcgccgta aagcagaacg tcatttagaa aaaggacgga tcgttatctt
     3901 tgcaggcggt accggcaacc cgtacttctc gacagacaca acagcagcat tacgcgcggc
     3961 agaaatcggc gcagatgtta tcctgatggc taaaaacaat gtagacggtg tttattctgc
     4021 tgatccgaaa gtagatgcaa atgcagtaaa atttgaagaa ctgactcatc tggaagttat
     4081 tgccaaagga ttacaagtca tggattcaac tgcaagttca ctaagcatgg ataatgatat
     4141 tccactgctt gtcttcaatt taaatgaaca cggaaatatt cgccgtgcaa tcttaggcga
     4201 aaatatcgga acaactgtaa gggggaaata aaaatggcag atgcaattat gacagaagca
     4261 aaagaaaaaa tgaaaaaagc agcacaaaat cttcaacgtg aacttgggca aatccgcgca
     4321 ggccgtgcga acgctagcct tttggaccgt attactgtga actactacgg tgcgccaaca
     4381 ccattgaacc aaatggcatc aatccaaatt ccagaagcac gtgttttaat gatcacacct
     4441 tttgacaaga gcattcttca agatgttgaa aaagctatta tggcaagtga tatcggaatc
     4501 agtccaacca atgatggaaa tgtcattcgc ttagttatcc cgcaactgac agaagaacgc
     4561 agaaaagaat tagctaaaga tgtgaaaaaa gaagcagaaa atgcaaaaat cgcgattcgt
     4621 aacatccgtc gtgatgcgat cgatgaatat aaaaaacaac aaaaaaatgg tgatattacc
     4681 gaagatgatt tgcgcggatt agaaaaagac gtacaaacat tgacagataa cagcatcaaa
     4741 gaagtagata agatcgctgc tgataaagaa aaagaattat tggaagttta ataaaatcat
     4801 aaaaa
//
LOCUS       DACEMO010000171         4719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEMO010000171 DACEMO010000000
VERSION     DACEMO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.176"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1138)
                     /gene="nagA"
                     /locus_tag="IW728_002300"
     CDS             complement(<1..1138)
                     /gene="nagA"
                     /locus_tag="IW728_002300"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1025641.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVER"
     gene            complement(1214..2050)
                     /locus_tag="IW728_002301"
     CDS             complement(1214..2050)
                     /locus_tag="IW728_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025642.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2234..3451)
                     /locus_tag="IW728_002302"
     CDS             complement(2234..3451)
                     /locus_tag="IW728_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1025643.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3834..3917
                     /locus_tag="IW728_002303"
     tRNA            3834..3917
                     /locus_tag="IW728_002303"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3868..3870,aa:Leu,seq:gag)
     gene            complement(4157..>4719)
                     /locus_tag="IW728_002304"
     CDS             complement(4157..>4719)
                     /locus_tag="IW728_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025644.1"
                     /translation="LLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFT
                     YVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDN
                     KKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMS
                     VQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1422 a          992 c          764 g         1541 t
ORIGIN      
        1 aacgttccac gccatctaga taagttgctt ccaatgtcat atcagggttc aatacgataa
       61 agtctgcatc gcgtccttga gcgatttttc cacatgtatc atcgatttta cagctgattg
      121 ctggaatcag actagccatc atgactgctt gttctggtgt tgctaatccc cagtctacaa
      181 catttttaat tgcttctttc aatttcaata tacttccagc taagttgcca gattctagac
      241 gtgctgttcc ttcttttact actactggaa attcgcctaa attgtaattt ccatcaggca
      301 tcccaccggc catcatacag tctgtaatta atgcgacatg atcatgtcct gctttttcca
      361 ttaggattcc cgcagcattt ggatgtacat gatggccatc acaaatcaat tcagaaaaga
      421 catgttctaa agataataac gcacccacca tacctggttc gcgatgattc aatccacgca
      481 taccattgaa agcatggaca aatacacttg cgcctgcttc aacagctttt tgcgcttctt
      541 ctagtgtagc attactgtgt cctagagaaa caactactcc ttcttcagtc acttgtttga
      601 caaactcttc tacaccttga cgttccggtg ctaaagcaat ttttttgatt aaaccacctg
      661 aagcttcttg ccattcatgg aaagtatcca aatcaggatc cccgaaataa cttgggtttt
      721 gtgccccttt gtgttcttct gtgaagaaag gaccttcaaa atagattcct ttgattttag
      781 ctcctggtgc ttcctgatac attttgccga ttgtctcagc tacatctttt aaacgttctt
      841 tgcttgacgt caatgttgtt ggtaaaaatg aagtcactcc acaagataac agaccttcag
      901 acatcacttt gatgccttct gcatcattat ccattacatc atgattcata tagccgtgaa
      961 tatgtgtatc taccaaacca ggagcaatcc atttgccgct ttggtcaatg atgttgactt
     1021 ctccctcagg tagttctttg gtgtattcac caaaaagtcc atcggtaatt tcaagatagc
     1081 ctgcatgttt gacacctgac gctaaaaaga atttgtcagc atagataaaa gttctcattc
     1141 tattctctcc ctttgttttt ctacctctaa agtactctta tttagagaag aagtcaagaa
     1201 tggtctacac catttacaaa ttttttgaaa tttgtttcac agcttgtgct aagccttcat
     1261 agaatgctgc tctttctggt tcttctttac gcttgtatat taagctttta agcacaaata
     1321 aatcattgag gtaatagtag ctattcttct cttgagccca atagattcct tgattcacct
     1381 gttctaacgc ttcgtcgatt tcattttgat ggaataagaa atttccataa ttataaaaaa
     1441 tttgcgaaaa ttcacttttt gatcgttcat cagctgattg atgcagcagt tggatgcttc
     1501 gactcaaata gtagcgtgct tcagccgttt ttcctgccac accatatatt tttccgatac
     1561 aactaatcag ttgtatttct gtgtcagaaa catggattct ttctttttgg ctagagtagc
     1621 ttaatgcttc tttcaaataa taaagggact gagagatatt ttgacgcaac gtgaattcgc
     1681 aaacaccgag ataataataa tagcgttgaa agtcagttgc ttgatacaga ttttcagaaa
     1741 tatcagaaga ttttagtact tgttctaact tccgataatc acgtttttgg aaacaactat
     1801 ccaacagttc aaataattta tcgtttgtcc gagtagtttt tcgagagaga ttcatcacat
     1861 gatcaatcgt tacccctagc cgttgacaaa tctgcatcat caccaataca ttcggcagtt
     1921 cctcattatt ttcgatccga cttaatacac tttgtgaaca aatatcatgc gaaagcattt
     1981 tttgcgtcat tttttgttct tttcgaatct ctttaatgat cgttccaaag gactcgatta
     2041 acgtttccat aagtatcctc cccaaaatat gcgtattcgc attctattcg tattttactt
     2101 taggaagtcg caaacttcaa gaaaagactt gcaactaaat aatatacttt agttatttaa
     2161 gaaaactcca ctagaaagag agcctcagtg tgtttgtctg tggccctcct actgaaaaat
     2221 ttggttttta aaatcatttt tggcgatagg ttgctaggaa ttctctcatt ttcttagctg
     2281 tttgtttcaa atcatccatt ttaggaagat aaacgattcg aaaatgatct ggctggttcc
     2341 aattaaatcc accaccatgt acaagtagaa tatgatgttc atgtaaaaag tccaaaacga
     2401 atttttcatc atccaaaata ttgaatcgct ttgtatcaat ttttgggaat atataaaaag
     2461 cagcttttgg ttttacggct gaaagaccag gaatatcatt gattgcatta tagatatatt
     2521 ctctttgttc atagatcctt ccgcctggca ggagcagctc atcgacactt tgataaccgc
     2581 ctagcgcggt ttggataatt tgctgagaca atacattgga acacaaacgc atggaggaca
     2641 acatattcag cccttctata tagcctttga cgtgtgactt atctccactt aggaccatcc
     2701 agcctactcg aaatcctgct acacgatgag atttggatag accgttcagc gtaacaacga
     2761 acaaatcagg cgctaatgta gcaatcggaa tatgttctag accatccatc actaagcgat
     2821 catagatttc gtcggaataa ataatcaggt tattttgtcg agctacttca acgatttctt
     2881 ctaataattc ttttggataa agtgccccag taggattatt aggattaatg ataacaatag
     2941 cttttgtccg actcgttatt tttgatttga tatcatcaat atcaggatac cattctgcct
     3001 cttcatcgca aatataatga acaggtgtgc ctccagctaa tgcaatagaa gccgtccaca
     3061 acggatagtc cggcattgga accagtactt catctccatt gtccagcaac ccctgcatac
     3121 acatcgtgat caattcgctc actccgttgc cggtgtagat atcattgatc gtcacattcg
     3181 gaaaatgttt cagttggcaa tattgctcaa ttgcttttct agcagaaaag atccccttgg
     3241 aatctgagta gccttctgag tcacgggcat tcatgatcaa atcacgaatg acttcgtttg
     3301 gtgcttcaaa tccaaaaggt gcgggatttc ccgtgttcaa ttttaaaata ctaatgccct
     3361 cttcttgcat ccgctccgct tcttctagaa cgggaccgcg aacatcgtaa ctgacacctt
     3421 ctaatttgtt ggatttttca aattctctca tcacttagcc aatccctcat ttcaaaaagt
     3481 tagaactttc caaatttttt gttatttact atttcaatat catactccat cctagaaaat
     3541 tttgccactt atttaggaaa aacgtaagta ttaaaaaaac acaaacaagc gttgagaact
     3601 taattgtcat gatagtcatt ccatagattc gctgcccaat ccattgcaat tccacaaatg
     3661 attcctagca taaaaccaag caaaaaacgt ccgaatgcaa gtccaatcaa tacacctaag
     3721 atcattccca gtcctgaaaa ttctccgtca tatttctcca tcttaataag tcctcccatt
     3781 tttgagcttg acaactatgt aaaacctcgc tatcattagt gtgttattat tacgcagatg
     3841 tgttggaatt ggcagacaag catgattgag ggtcatgtga gcgttagctc gtgtgggttc
     3901 gagtcccatc atctgcacta caactcgtga agttaatttt gcttcacgag tttttttgtt
     3961 tgaacgtttc tttatgccat tatatacata tcactgtatc ctttacaaaa agaacgtaca
     4021 atcatgtaaa cagcctgagg tgagtatatg aaggtcggaa acaagctact cgtagaaact
     4081 tgcaagtgtt aaaacaatta atcgaaaaac gagtaaaatc cctcacctaa tactaaagcg
     4141 agggattttt tgtatattat ctgtttatca ccgttattat aaaggtacaa ataccaagca
     4201 cggttaatcc aaattgcata aattcatttt tttgtacact catgcttttc attagctctt
     4261 taactgccat tagtactaag atggcgataa caacataaag tatatagtac gtccgcattc
     4321 cggaagccca tactgtggga gaaaatccca taattaacct tgaacaaaat cccattatca
     4381 tcaaaataac taaaaaaata gaagttttct tattgtcaaa acttaaataa attccaacaa
     4441 ttaaacatag taataatgcc aagactacta aagtagctat ccaagtacct ggataaaaaa
     4501 gtgacagttt cgttcctaac tctgtaaaga gattattcag gttactacta ctccaaatca
     4561 ttgccctttt atttccattg acgtaagtaa agctttgacc cattgtatta ccaaaataaa
     4621 taatcaggtt taagaaaaac ggtaatgcag tcaatatacg cacataatag ttttgacatt
     4681 tcctatatgt tagaaggaaa attaagaaaa atagcaata
//
LOCUS       DACEMO010000172         4719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEMO010000172 DACEMO010000000
VERSION     DACEMO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.177"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            162..386
                     /gene="nrdH"
                     /locus_tag="IW728_002305"
     CDS             162..386
                     /gene="nrdH"
                     /locus_tag="IW728_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1025645.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            545..2704
                     /gene="nrdE"
                     /locus_tag="IW728_002306"
     CDS             545..2704
                     /gene="nrdE"
                     /locus_tag="IW728_002306"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1025646.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2754..3719
                     /gene="nrdF"
                     /locus_tag="IW728_002307"
     CDS             2754..3719
                     /gene="nrdF"
                     /locus_tag="IW728_002307"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1025647.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3808..>4719)
                     /locus_tag="IW728_002308"
     CDS             complement(3808..>4719)
                     /locus_tag="IW728_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1025648.1"
                     /translation="LLTFLVVIILVVLTVMNVVPQLMEQTIQLTQSLPMYADETSKWL
                     NELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASVSKIISFMMQFFILLFTVP
                     FILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETLSAYISSTILDALIIGVMS
                     FLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIVGLFISPFQALYMALSILV
                     IQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILGMLICIPVYAVIKTIILNI
                     RKIYLLRRMEHVSEKLDSPKVLEETSE"
BASE COUNT         1611 a          877 c          845 g         1386 t
ORIGIN      
        1 cccacacgat acttgttttc agctcttttt tcaaaaacac aaaacttagt attccacagt
       61 ttccacaaca caatatgtag tgtttgttgt ttgacttttt tactaagagt tttatactat
      121 ctattataga taataagaat gtgttagagg aggcactcac catgaacgta aaaatctttt
      181 ctaaaaataa ttgtatgcaa tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg
      241 cttttgaaga agtcaatatc gatgaacagc ctgaagcagt agactggttg aaagcacaag
      301 gattccaaag tgttcctgtc attacttctg atgcagaaac agttatcgga ttccgtcctg
      361 atcagttgcg taaacttgcc agctaacatg actaaaagaa tcaaaacgtg atacatggat
      421 ttgaagggct tgagacagca ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct
      481 taaaaacaat gcaatgattt gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt
      541 acgtatgagt cttaaaaatt tacaagatgt cagttatttc aagctgaata atgaaatcaa
      601 tcggccagtc aatggtcaaa tccctttgaa taaggataaa gaagctttag ttgccttttt
      661 tgaggaaaat gtcaaaccaa atacgatgac ttttccatct attatggaaa aaatcaatta
      721 cttgatcgat caagaatata tagaagcaga attcattcaa ttgtattcac catcatttat
      781 tgaaaagcta tatcaatttt tggacgagca acatttttct ttcaaatctt ttatggctgc
      841 ttacaaattt tattctcaat atgcgttgaa aaccaacgac ggttccgctt atctggaaac
      901 ttatgaagat cgagtagcct tcaatgcgct ttactttgcg aacggggatg aagaattagc
      961 acttgctctt gcagacgaaa tgatccatca acgatatcag ccagctacac cttctttctt
     1021 gaatgctgga cgtaaacgtc ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga
     1081 cgatatgaac tctatcgggc gctcaatcaa ctctgctttg caattgtccc gtattggtgg
     1141 cggcgtaggg attactttat caaatcttcg tgaagccggc gctcctatca aaggatatga
     1201 gggtgctgca agtggtgtcg ttccagtcat gaaattattc gaagatagtt tcagctattc
     1261 taatcaatta ggtcagcgtc aaggagctgg cgtggtttat ttgaacgttt tccatccaga
     1321 cattattgct ttcctttcag caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac
     1381 tctttctctt ggcgtgatcg tacctgataa attttatgaa ttgacacgta aaaacgaaga
     1441 tatgtattta tttagtccat atagtgtcga aaaagaatat ggtgtcccat tctcttatgt
     1501 aaatattaca gctgagtatg atcatctagt tgcaaatcca aacattcgca aacaaaaaat
     1561 caaagcgcgt gatcttgaaa atgaaatctc aaaattacaa caagaatctg gctatcctta
     1621 cgtaatcaat attgatacag cgaaccgcga gaatccaatc gatggcaaga tcatcatgag
     1681 taatttatgt tcagagattt tacaagttca aacgccttct gtgattaacg gcaaacagga
     1741 atatgaagta ttaggaacag atatctcatg taacttaggc tcgacaaata tcgtgaattt
     1801 gatgcaaagc cctaactttg gtcaatctgt gcgtacgatg actcgtgcat tgacatttat
     1861 tacagatgca tctgatatcg atgtcgtacc accgatccaa aacggaaata agctgaatca
     1921 tacgattgga ttaggtgcca tgggcttaca cacttatctt gcaaaagaac aaatggaata
     1981 tggctcagag gattcattag actttacaaa tatttacttc atgctgttga attattggac
     2041 acttgttgaa agcaacaata tcgctcgtga aagaaaacaa gttttccata attttgaaaa
     2101 atcagcttat gctgacggca cttatttcga aaaatacgta actggagaat tccagccaaa
     2161 atcagaaaaa gtcaaagcat tgtttgaagg catttttatc ccaactgctg aagattggaa
     2221 tgctttgaaa caagcagtaa tgaaagatgg actgtatcat caaaatcgct tggcagtagc
     2281 accaaatggc tcaatttcat atatcaacga tacgagtgca agtattcatc ctatcactcg
     2341 catgatcgaa gaacgccaag agaagaaaat cggtaaaatt tattacccag cgccttatct
     2401 atccaatgag acgattccgt attacacttc tgcttatgat atggacatgc gtaaagtaat
     2461 cgatgtttat gcaacggcac aacaacacgt cgatcaaggc atgagtttga ctttattcat
     2521 gcgttctgaa atcccagaag gcatttatga atggaagaca acaacgaagc aaacgacacg
     2581 tgatttgaat attttaagac attacgcttt ccacaaagga atcaaatcca tttattacgt
     2641 acgtaccttt actgatgatg cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat
     2701 ctaatatcat agatagattc gcttattcat attttaatta aaggagcatt ctcatgtcag
     2761 aaacatatta tgaagcaata aactggaatg aaattgaaga tatcattgat aaatccactt
     2821 gggagaagct gacagagcaa ttttggctag atacacgtat cccactttca aatgacctag
     2881 atgattggcg tacattgtct caattggaaa aagatacagt aggacatgta tttggcggtt
     2941 tgacactgct agatactgtc caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc
     3001 gcactcccca tgaagaagcc gttttgaaca atattcaatt catggaatcc gttcatgcca
     3061 aaagttattc ttctattttt agtacactga atacgaaaaa agaaatcgaa gaaatttttg
     3121 actggacaaa tacgaatcct tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca
     3181 aacacggtac accgctagaa aagaaaattg ccagcgtctt tttagaaaca ttcttgttct
     3241 attcaggttt ttatactcct ctttattatt taggaaacaa caaattggct aacgtagcag
     3301 agatcatcaa actaatcatc cgagatgaat ctgtacatgg aacctacatc ggttacaaat
     3361 tccaattagg attcaacgag ttacctgaag cagaacaaga aacgttaaaa gactggatgt
     3421 acaatcttct ttatgagctt tacgaaaacg aagaacgtta tacagaagaa ttatacgatc
     3481 caatcggctg gacagaagaa gtcaagacat tcctacgcta taatgccaac aaagcattga
     3541 tgaacctagg gatggatcca ctctttccag atactgccaa tgatgtcaac ccaatcgtaa
     3601 tgaacgggat ttcaacaggc acaagcaacc atgacttctt ctcacaagtc ggaaacggct
     3661 atttgttagg tcatgtagaa gcgatgaaag acgacgatta tttgattgga ttggaataag
     3721 gatttatttt aattcaaaaa tttttcgaaa aataaaaaga gtttagcgta cagatatttt
     3781 aaatgtacac tgaactcttt tttatgctta ttcacttgtt tcttccagca cttttggact
     3841 gtctaacttt tccgaaacat gttccattct tctcaataaa tagattttcc ggatatttaa
     3901 tattatcgtc ttaatcactg catagacagg tatgcaaatc agcatgccta atattcctgc
     3961 gacacttcct gctccaatca ataccaagat gatcgtcaaa ggatggatat tcattgattt
     4021 cccaaataac aacggcttga tcaaatttcc atctaattgt tggatcacta aaatcgacaa
     4081 cgccatatac aaagcttgaa atggagagat aaacaaaccg acaatgattg ctggtaccgc
     4141 tccaataaaa gggccgacat acgggataat attcgtgatt ccgcaaatga ccgcaagcaa
     4201 caagctataa ggctgatcga atattctcat tgccaaaaag ctcattacac caataatcaa
     4261 agcatctaag atcgtactgc tgatataggc agataatgtt tcgtttaatt cttttaccgt
     4321 ttggcgtaac tctttacgaa tcgctttcgg aaagaagtga gacaacgcat caataaattt
     4381 gtgcccatcc ttgaacataa atagcaaaat aaatggaacg gtaaataaca aaataaaaaa
     4441 ttgcatcata aatgaaatga tttttgagac acttgcagat aaactgacta atatgatatt
     4501 gaacaaatta ctaagagata aatttgctga tgctaattgt tcttccaaat tgaaattttt
     4561 aaactcttcc cgctgcgcca attcatttaa ccatttgctt gtttcatctg catacattgg
     4621 caagctctgg gtcaattgaa ttgtctgttc cattaactga ggcactacat tcatgacagt
     4681 taaaacaact aaaatgatta cgaccaaaaa cgtcaataa
//
LOCUS       DACEMO010000173         4634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEMO010000173 DACEMO010000000
VERSION     DACEMO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.178"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            69..1496
                     /locus_tag="IW728_002309"
     CDS             69..1496
                     /locus_tag="IW728_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1025649.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1588..1953)
                     /locus_tag="IW728_002310"
     CDS             complement(1588..1953)
                     /locus_tag="IW728_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1025650.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1950..2315)
                     /locus_tag="IW728_002311"
     CDS             complement(1950..2315)
                     /locus_tag="IW728_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1025651.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2507..2755)
                     /locus_tag="IW728_002312"
     CDS             complement(2507..2755)
                     /locus_tag="IW728_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025652.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2754..2882
                     /locus_tag="IW728_002313"
     CDS             2754..2882
                     /locus_tag="IW728_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ1025653.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2939..4633
                     /locus_tag="IW728_002314"
     CDS             2939..4633
                     /locus_tag="IW728_002314"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ1025654.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
BASE COUNT         1527 a          884 c          817 g         1406 t
ORIGIN      
        1 tttcaaaaag aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag
       61 gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg
      121 ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt
      181 gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg
      241 cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat
      301 gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt
      361 tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca
      421 aagcttattc attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg
      481 accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt
      541 atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc
      601 gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa
      661 tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag
      721 attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat
      781 gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac
      841 tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac
      901 agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg
      961 aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc
     1021 tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa
     1081 atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc
     1141 agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga
     1201 gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg
     1261 ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg
     1321 caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc
     1381 gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc
     1441 ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg
     1501 ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat
     1561 ctcttgatac acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc
     1621 tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat
     1681 atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa
     1741 acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc
     1801 gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac
     1861 accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata
     1921 tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct
     1981 ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt
     2041 gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg
     2101 actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta
     2161 cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga
     2221 acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc
     2281 cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt
     2341 tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct
     2401 aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca
     2461 taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca
     2521 gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac
     2581 catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag
     2641 acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta
     2701 ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta
     2761 aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag
     2821 gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct
     2881 agcatcctat ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat
     2941 gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta
     3001 tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca
     3061 cggttcaaat gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca
     3121 tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac
     3181 ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac
     3241 tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt
     3301 gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg
     3361 tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat
     3421 cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat
     3481 gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat
     3541 gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg
     3601 ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg
     3661 caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga
     3721 tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt
     3781 tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag attcagctac
     3841 tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct
     3901 ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga
     3961 tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa
     4021 tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac
     4081 cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca
     4141 accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc
     4201 aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct
     4261 agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa
     4321 agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc
     4381 cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac
     4441 caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca
     4501 aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg
     4561 tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa
     4621 aatgtttgaa taat
//
LOCUS       DACEMO010000174         4590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEMO010000174 DACEMO010000000
VERSION     DACEMO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.180"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            300..1433
                     /locus_tag="IW728_002315"
     CDS             300..1433
                     /locus_tag="IW728_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ1025655.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            1464..1784
                     /locus_tag="IW728_002316"
     CDS             1464..1784
                     /locus_tag="IW728_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025656.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(1813..2277)
                     /locus_tag="IW728_002317"
     CDS             complement(1813..2277)
                     /locus_tag="IW728_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1025657.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(2409..3662)
                     /locus_tag="IW728_002318"
     CDS             complement(2409..3662)
                     /locus_tag="IW728_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1025658.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(3691..4008)
                     /locus_tag="IW728_002319"
     CDS             complement(3691..4008)
                     /locus_tag="IW728_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1025659.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(4034..4354)
                     /locus_tag="IW728_002320"
     CDS             complement(4034..4354)
                     /locus_tag="IW728_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1025660.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         1457 a          932 c          820 g         1381 t
ORIGIN      
        1 tcccttctgt cataacctct ttttatttat gtctgtttct ttttgaaaac aactattttt
       61 tcaacgttta ctctcttgcc aatccaacat catctaacgg atacaactat ttcaatgatt
      121 accattacac taaaaaatcg gaaacacacc tctccgaaca caaatacata agactataga
      181 atgtttcatt taagaaaaaa agcgtttcat tatcaaaaag gtaagatctc ctttaaaatg
      241 aaattatctg tagtactaat aatgcagaca attttatgaa tctgaaagga gcatctccaa
      301 tgaaagcagt cacttggcaa ggaaaacaga aaatggaaat cagaaacgta gatgatccaa
      361 aaatattaga accaacagat gcaatcatcc gtatcacagc aacagccatt tgcggctccg
      421 atctgcatct ttaccatcat ggtgaagcag ttttggaaaa agggtatgtt gtaggacacg
      481 aaccaatggg aatcgttgaa gaagtgggat ctcaagttca aacattgaaa aaagggcaac
      541 gtgtggttat cccttttaat attagttgtg gtcattgtca tttctgcctg aatcacatgg
      601 aaagccaatg tgatgaatca aatccagatc agttatatgg cgggcttttc ggatttggca
      661 agttgaacgg taatcattgg ggtggtcaag cggaatatct aagagttcct tatgcagact
      721 ctacttcatt tgttgtacca gataatgatc ttcctgatga aaaagtttta tttctatcag
      781 atattttgcc cacagcttat tggagcgttg aaaacgcagg agtaaaaaaa ggcgatacag
      841 ttgtgattct aggttctggt ccagtcggtc tattcgctca aaaattcgct gttatggctg
      901 gagcagagcg cgtaatcgca gtagatccag tacagcaccg tctagataaa gcggctaatt
      961 acaatggggt tgaaacttat ttcttagaag acacggcaca agctggaaca gaattgtatg
     1021 aactggctaa aggtggcgca gatgtcatta tcgattgtgt aggaatggac ggattagagc
     1081 ctgtcaaaga aaaagcgaaa aatttagtaa gcttacaaag cggcacaatc tctccattac
     1141 agatggcatc gcaagctgtc aaaaaatttg gaacagtcca agtaactggt gtttatatga
     1201 ctccagcttc ttcttacccg ctgcaagaat tttttatgcg ggatatcgct gtcaagcatg
     1261 gacaggcacc aattatccat ctaatgccaa aaatttacgc aatgattgca gaaggactat
     1321 tcgatcctac acaaatcatt actcattcgc tgccactatc agaagcagca caagcatatg
     1381 agatctttga caaaaaagaa gataaaaata taaaagttgt tttgaaacct tgaattcagt
     1441 aaaaaataca ggtgattttc tttatgccca aaatcaaacg aaatacccat gagatcgaaa
     1501 taggaaaaga agaagatatc gaaatacgag gacgtcgctt ccgcttctat ttccaaaatc
     1561 gttacacatt gatctcactg gctgtcgatt tattaaccgg gattttctat atttttggaa
     1621 gccttgcttc cttaactaaa atccccgatc gatatggcac ctatttttat ttggctggtg
     1681 ctattttctt gacgattcgt cctattctca gaatcgtaag gaacattttt atttacgacg
     1741 aaaaaaaagc aatgaagaga acaagcaaaa aaaaagaggc atgagaattt ttcaaatctc
     1801 atgcctcttt tttcacccta tcgtatcact ttgatacaga cgagcggtcg tcccactttc
     1861 ttccttgacc attgtcaaaa atgtccactc gtatcgttca taaaacgtgg tatgatctgt
     1921 aattaagtaa agtttttcgt acccaagttg atgcatttct tttctgattt ccgctaatat
     1981 ccgcttggct atcccctgct tacgcttttc cggttcaaca aacagggcac aaagatttgg
     2041 tgtcagatca gggcggttat ggaagtcgtt ttcaatgacg cccgctcctc cgacgatggt
     2101 ttctttttca tctaagacca cataccattg aggaatactt tccttattag cgattccagt
     2161 acgaatactt tcctcataca caacagcagg aactccccat tttgatgaaa accaattcgc
     2221 gctttgtttt actaattctg ggtgttctct taatttgatc cagttatatg taaccaatgt
     2281 ttaaacctcc gactaaaata aaacgtttac aaaaattata caagtcttta tgaaaattag
     2341 caatgacaac aatagtgatg taaaaaaaaa aacggaaaac aatcacttgt tctccgtttt
     2401 ttgattaatc aagcatctaa agcttctttt cttctttctt ggatcgtagc taccttgacg
     2461 aacggtagat agatcaaaat agaaagaacc aaattgacag cggctagtac accacctgca
     2521 atactttttg ttgccaatac accgccaata attggtggag ttacccatgg aggcataacg
     2581 gtagctgctg gcactaaacc tgtagaagtc gcaaagtaag caacactaac taagaccatt
     2641 ggtgtcaaaa taaatggaat gaacatgatc gggttcaaca cgataggtaa cccaaacatc
     2701 gttggttcat taatattgaa gacacctggc gcaatcgaca agtttgttac aaccatatat
     2761 ggtttgtttt tacgtcctac taaataaata gctaacagta aacctaaagt tgcacctgtt
     2821 cctcctaagt tgacaaagct gtcaaagaaa ggtttgttca cgataaacgg tgcgctatgt
     2881 ccagctgcga ttgcattaac gtttgcttca atggctggcg cattgattgt ttgcatcaaa
     2941 ggatcgacca tattcgctcc gtgtaaacca aagaaccata agaatggtgt gataaatgca
     3001 agcagtaagg cagatggata agagtttgca agtcccataa atggttcttg gaccactgta
     3061 tagaaagaac ctacgatatc atcaacaccg aatgcagcaa aaattgcagc aactaaagca
     3121 aacgcagaga tagtgatcat tgctggtagt aatgaagcaa atgcttttgc aactgccggt
     3181 ggcacaccat caggcatttt gatgatcaat ttttcatttc cgcttaaacg tacaaagatt
     3241 tctgttgaaa taatcccaac aataatagca ataaacaaac cgtttgagct catacctaat
     3301 gctccgccta aagcgaagaa agagcctaaa gaaatcacac ctgcggaaac accgtctttt
     3361 cggtagccgt tagctaaatg ataagcaagc aagaaagcaa tcaagacaga aagaatggca
     3421 aatgtaccgt tccaagcagc agccccgaat cctttccatg attcaccacc aaaaattgag
     3481 ttcatcaatt cttggaaccc gggaatcggt aagttattga tcattactgc taatgctcct
     3541 aagatcatta acggcatact caccataaat gagtcccgaa tcgcaactaa atgccgttgt
     3601 gcaccaattt ttgatgctac aggtataaaa tgtttctcca taaatgcaac aaatttatcc
     3661 ataaatcttc cctcttttct ttactgtaga ttacgcgttc aatcgacgat acagatcaat
     3721 gatctctttt gctaaatctg taaaagctat agatgtcatc agatgatctt gactgtggac
     3781 cgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct tgtgtcagca tctctgtttg
     3841 agagtgatgt gcttggacta aagattcctc tgcatctttg attttttcat ctgctaaagt
     3901 gaaatcacct tttttcgcag cagcaatcgc ttccattgca tcgctttttg catttccgcc
     3961 aaacatgatc agacccatga tggcttctag atttttttga tcttccattt attgtatcct
     4021 ccgataattt ttattatttg tttattagtt tttctgcttg gtcgagtact ttttcaccat
     4081 tcatcatgcc atagtcagcc atattgatta catctaaagg aatgccttta ggtgctagct
     4141 tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc taaaagtaaa acgtctacat
     4201 ttttagattc tagttgatta tccgcatctg aagcagatac agcaaagatg tctgcttcca
     4261 ttccttttgc ttctgctgcc ttttgcattt ttgtgactag gagacttgta ctcattccag
     4321 ctgaacatac aagcatgatt gttttctttt ccattttaaa cacgttcctt tctatactta
     4381 cacttgtata taagcaagta gcgtgccaac tttaaaagcg ctttaaaaag ttgatatcat
     4441 cacatttaag gtctatacca agttgcatac acactaaaaa agaaagcgca cactaaacac
     4501 acaaattaat ttttattacc ttttttcttt taacgtgcac aaaaaaagtt gtgacacaaa
     4561 acttgtcaca acttttttca aataaacggt
//
LOCUS       DACEMO010000175         4534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEMO010000175 DACEMO010000000
VERSION     DACEMO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.181"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            274..1611
                     /locus_tag="IW728_002321"
     CDS             274..1611
                     /locus_tag="IW728_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1025661.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1659..3788
                     /locus_tag="IW728_002322"
     CDS             1659..3788
                     /locus_tag="IW728_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1025662.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3763..4452
                     /locus_tag="IW728_002323"
     CDS             3763..4452
                     /locus_tag="IW728_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025663.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
BASE COUNT         1425 a          802 c         1009 g         1298 t
ORIGIN      
        1 aaaagaagct ttggctaaat aagctaaaca attataaaga ggaaagaaga taacgaaaag
       61 ttatgttctt tcctcttttt ttatttacaa tcctccatca aatgaaacag gacaataacg
      121 tataagtcta aaatagcata attaaggttt tttaattgaa aactgaacat ttatggtcac
      181 cctttaatga aagcgctaac tacataagtt agaatgagtt tgactgatat attagtttgc
      241 ttatcagaaa aacaaaatgg acggaggaat aacatgagtg atattattat tggtattttg
      301 ttagttatct cattcttttt catggtctgg tactgtgtaa aaggctacaa cttgatggtc
      361 ggctttgcca tcatggctac tgtatggatg ggattagcgc tagtcggaaa cacgttttca
      421 ccaaacccgg cgatggaagg gcaaggagta attgatgtgt tgacgcatat ctatacgacg
      481 gggccagctg agtacgccaa atcgatcttg gtaaatgtct tctttggtgc ctttttcggg
      541 cgtgttctag tagatagcgg aatcgcagct accctgattc gaaaggtagt tgaacttgga
      601 ggggacaagc caagaatcac gatgagctta ctatgtattg taaccgctgt tatattcatg
      661 tcgatgacag gaatcggacc agtgatttct attgcagtta ttgtgttgcc aatcttgatg
      721 tcattaggta tttcagtacc ggttgcactg ttctcattta tgggctcgat tatggctgga
      781 atctttgcta atatcgtcaa tttcaaacaa tatcaaacga tttatgctgg tttcaatcca
      841 gcggctgaaa gctatactta taatgactat ttccaaattg gtatgattgg gatgattgta
      901 agcttagttg tggtactaac ggtcgctaat atatcaatga acaaaaagaa acgctatgca
      961 atggcagcaa acgtaccagc tgaaggtggc gacgcaccaa tgatctcatg gttagccgta
     1021 ttattaccag ttttaggggt cgttctttta gatcttccaa tcatcttagg ttttattcta
     1081 gcaggtatct gggcattgct gtttactgga aaattgcgcg gaggttataa agaaatctgt
     1141 cgtcagtttg cgaaattatt tacagatggc gcagtagatg ttgcgccaat ggttggtttc
     1201 ttgatgacct tagcgatgtt caataattca gctgcgtatg cttctcctta tttttcagct
     1261 atttttggag attggatacc gcaatcacca ttagtcttgg caatcgtatt tgctatattg
     1321 acaccactag gatttttccg cggtccaatg aatctagtcg gttcaggctc agctatttta
     1381 gctgtcgtat tagctgttaa tccaacgatg tcgccagcct ttttgttccc gttatttgca
     1441 atcacaacga ttgctcctca gcatttagat attacacaat cttgggttgc ttggggatta
     1501 ggttatacaa aagtaacttc tcgagaatat atgaagaaat ccatcccaac tggttggatc
     1561 atcggagcga tcttatgcct gatcacgttc ttattgtacg ggaacgccta gtaaacaaaa
     1621 cgtcgataaa aacgattaga tgaatggagg aaaagaaaat gaaacgtttt gtgcgaatgg
     1681 gaattgatgt tggcggtaca catacgaaag cagtcgcaat cgataatgcg acgcacgaaa
     1741 tcatcggaaa atcttcagtg aaaacaaccc acgatgatgt ccgtggagta gccgcaggtg
     1801 tcgttcagtc ttttcaaaat tgtttgctgg aaaataatat cagtccagaa gatgttgtgt
     1861 ttgttgcaca ttctaccaca caagcgacta atgcactgat tgaaggagac gtagcaaaag
     1921 tcggcgtaat cgggatggca aaaggtggat tagaaggttt tttagcaaaa cgacaaacta
     1981 gattgaacga tatcgattta gggaataaga aaaaaataga gattgtcaat gcattcttac
     2041 ctgtcaaaca cttaaatgta gaccgtgttt cagaaacgat ttcttcttta gaaagagaaa
     2101 gagcggaggt cttggtttct tctatggcat ttggagtaga taatggggaa ccagaacgag
     2161 ttgtttatga agcagcatcg gtaaaatcga tccctacaac gatggcttct gacatcacaa
     2221 aattatatgg attgacaaga agaactcgga cagcagcaat caatgcatct attttaccaa
     2281 aaatgctaga tacggcaacc tctacagaag attccgtacg agaagcaggg gtcaatgttt
     2341 ctttgatgat tatgcgcgga gacggcggag taatggaaat caatgaaatg aaaaaacgac
     2401 ctgttttaac catgctttcc ggtccagcag catcagtcat gggctctttg atgtacttga
     2461 gagcgtctaa tggtgtttat tttgaagtag gcggaacaac aaccaatatc ggggtcatta
     2521 aaaatggacg tccagcaatc gattattcaa ttgtaggtgg ccacccaaca tatatctctt
     2581 cattagatgt tcgggttcta ggtgtggcag gcggttcgat ggtacgggct aatcagtcag
     2641 gaatcatcga tgtcggtcca cgttctgcac atattgccgg tctagattat gcagtattta
     2701 cggagacaga aaaaatcaaa ggtcctaagg ttgaattttt ctctcctaaa gaaggagacc
     2761 ctgctgatta cgtcaaggta gtgatggaag acggagaaga agtgactatt acaaatacat
     2821 gtgcagccaa tgtccttgga ttagtgcaag aagaacattt ttcttatgga aatgtcccat
     2881 ctgcacgaaa agccatccaa gcattagctg attattgtca tacgacggtt gaagacatcg
     2941 cagaacagat catggaaaaa tcctatgcta agattgaacc ggtgatttta gaattggcag
     3001 acaagtatca cttggagaaa gatcagattt ctcttgtagg tgtagggggc ggcgctgcct
     3061 cattgattac ttactttagt aataaaatgg gtgtgaaata ttcgattcct gaaaacgccg
     3121 aagtgatttc ttctattggt gtagcacttg caatggtccg agatgttgta gaacgtatca
     3181 tcccttcgcc aagtaaagaa gatatacgat cgttgaaaaa tgaagcgatg aataaagcga
     3241 ttgaatcagg cgctacacca gaatcgatcg aagtccacgt agaaatcgat ccacagactt
     3301 caaaagtaac ggctatcgct actggttcta cggaagtgaa agcaaccgat ctaaccaagg
     3361 agatcacaac agaagaagca ctagagcttg ctgcagagga tatgcgatta aataaaaatg
     3421 aagtttgctt attggaaaat acacctttct tttatgtatg cggcgaacag aatcgttcaa
     3481 aaaatgctgg aagtcttcgg atcatcgatc aaaaaggatt tatcaaggtc caacgtggac
     3541 atgcttcatg tatgaaaaca actgcagcca attatatgac agctgtcgaa caactttggg
     3601 aagatatggc agtttatcaa actgaattga ttgcccgacc agaattttat ctctgcttag
     3661 gagcaagagt ctctgatttt acagcgacgg atctggaaca actacagctt ttgatggatc
     3721 tggaagtatc tactatggaa ccggaggaag aagtgatcgt cgttgccgga aatatcaaac
     3781 aaacataagt tagaagagtt actaaacgaa ttacaacaga tccccgaaga aatttgggga
     3841 ttctaccagt ttcggcgaga tttattttgg aaaaagattc ctttgtccaa gcagaaaatt
     3901 cttatacaac aatcaatcga ttgtggtatc gaaacggcat gtagcatcaa aaaaaagtac
     3961 ccttttgccg atgtaggaga aatttgtgaa caaatggctg ttccaatcgt ttcctgtgaa
     4021 tcagagcaaa taaacgaacg aatcactttt gcaacttatg cagaagatga agggattcga
     4081 ttgatgacgg agccgttaga aaaattaaaa tgttcaggac tagcttcgat ctctaaagaa
     4141 acagcgcaag ctttgattat tggacatgaa ctatttcacc acatcgaagc ttctgttaag
     4201 ggaatttata cgcaaagcga aaaaattgtg ctgtggcggt tgccttttta tacacatcaa
     4261 tcaaacattc gtgcattgag cgagatagca gcgatgagtt tttcaaaaga gatgaatcaa
     4321 agcgattttt cgccttatgt attagaggca gtcttacttt ggccatataa tgaaattcgt
     4381 agccaaggga tcttagaaga aataaaaaaa atagaaaaaa gatgtacgga gtatgattta
     4441 gattacaaat aaagagactg tgacaaaagt cgtgtcacag tccttttttt ccgaataaac
     4501 gatgttctcc tgtcaactcc tcggtcacaa gtca
//
LOCUS       DACEMO010000176         4533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEMO010000176 DACEMO010000000
VERSION     DACEMO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.182"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            70..153
                     /locus_tag="IW728_002324"
     CDS             70..153
                     /locus_tag="IW728_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1025664.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            278..1015
                     /gene="erm(B)"
                     /locus_tag="IW728_002325"
     CDS             278..1015
                     /gene="erm(B)"
                     /locus_tag="IW728_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1025665.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1185..1445
                     /locus_tag="IW728_002326"
                     /pseudo
     CDS             1185..1445
                     /locus_tag="IW728_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1608..2273
                     /locus_tag="IW728_002327"
                     /pseudo
     CDS             1608..2273
                     /locus_tag="IW728_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2309..2554
                     /locus_tag="IW728_002328"
     CDS             2309..2554
                     /locus_tag="IW728_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1025666.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2657..3526
                     /locus_tag="IW728_002329"
     CDS             2657..3526
                     /locus_tag="IW728_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1025667.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3507..4241
                     /locus_tag="IW728_002330"
     CDS             3507..4241
                     /locus_tag="IW728_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1025668.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4274..>4533
                     /locus_tag="IW728_002331"
     CDS             4274..>4533
                     /locus_tag="IW728_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HAQ1025669.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKG"
BASE COUNT         1626 a          664 c          884 g         1359 t
ORIGIN      
        1 tttgtgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
       61 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
      121 cagactaaaa acagtgatta cgcagataaa taaatacgtt agattaattc ctaccagtga
      181 ctaatcttat gactttttaa acagataact aaaattacaa acaaatcgtt taacttctgt
      241 atttatttac agatgtaata acttcaggag tgattacatg aacaaaaata taaaatattc
      301 tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa
      361 agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc
      421 taaaataagt aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc
      481 agaaaaatta aaactgaaca ttcgtgtcac tttaattcac caagatattc tacagtttca
      541 attccctaac aaacagaggt ataaaattgt tgggagtatt ccttaccatt taagcacaca
      601 aattattaaa aaagtggttt ttgaaagcca tgcgtctgac atctatctga ttgttgaaga
      661 aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca
      721 agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt
      781 aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa
      841 gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa
      901 aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta ccgttactta
      961 tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta
     1021 tgagtcgctt ttttaaattt ggaaagttac acgttactaa agggaatgga gataaattat
     1081 tagatatact actgacagct tccaagaagc taaagaggtc cctagcgcct acggggaatt
     1141 tgtatcgata aggggtacaa attcccacta agcgctcggg accccttgta ggaaaatgtc
     1201 ctaagtgtgg caacaatatt gtattaaaaa aatcgtttta tggttgttca aattatcctg
     1261 aatgtaagtt tactttagct gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa
     1321 aagaattact ggagggaaaa gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc
     1381 cctacaatgc cgttgtaaaa attggggaaa agggatatat tgattttatc tctttttcaa
     1441 aataaacatg gggctgaccc aaaagtagct cctatatgaa aaatagaggt ctttccattt
     1501 agatagtagt ggaaagacct ctatttatct tttgaaagtg aatacaaaaa taaagcacac
     1561 tctgctataa tataggtgat taatccattt gagagaggtg ctttatcatg tatcaaaatt
     1621 ataccataaa ccaactttgt ttgcctatag atcttgaaat taaattagaa gaaaatgatt
     1681 ttgctcatgc cattgtgcag tttgttgact ctattcccga tgaagtcttt cttccttatt
     1741 atcaatctat gggtagaccc caatatcatc ctcgtatgct actttccatt attctttgtg
     1801 cctatattca aggggtttat tcgggccgcc aaatacaaaa tatgctgata gatagtattc
     1861 ggatgcgcta cttatctcaa gagcaattcc ctaactttcg tactattaat cgttttcgcg
     1921 ttcatccgat tatgaacgat attctagatc attcatttgt tcaatttcgt gagctactgg
     1981 ttcaaagtgg cttaatcagt ggcagtgctc tatacattga tggtacaaaa attgaagcgg
     2041 atgcgaataa atactctttc gtttggaaaa aaagtatttt aaagtataag gatagccttg
     2101 atcaaaaagc ccttgaaaac taccaacaaa tggtggaaac aaaaatctta cctgagttaa
     2161 tagatgattt aaaggatgaa ctttctatag aagagattga aaggattcgt caatctttgg
     2221 aagaaaagga aaatcaatta attgaagcga ttgaccaaac cgaaacagta gaagggggct
     2281 gacccattat taataaggag tggacaacat gattgatgta aatgagctat tggaagaagc
     2341 tattagagaa actgaaaatc taaatgatgg cgaagttttt cttgttaaag atttattcaa
     2401 gggatatgtg gggaatagga tacccagaaa ggatcgactt ctgcttggaa cgttattttt
     2461 aaaccatgtt agcaaaatga atggtaattt aaaggctatt gaaaagactt catctaatca
     2521 gcagaggtat aaaaagacaa ttgataagtt ttaactatct tttgtgaaat taaaaaaatt
     2581 ataaatatgt tgagtagaag caaaagattt ggcttggaat ttttacagca gtgaatcgat
     2641 aaaaggagca tgcttgatgg tagataatat tattaaatca gtagcagaga aattatcctc
     2701 tctgtcttat atagaaggta ttgttttagg tggttcacgt gcaaggggca cccatacaga
     2761 ggattcggat atagatatcg gcatctatta caattcagaa tcatttgaca taaatactat
     2821 taatcaattc gctacaaagc tggatgatga gcatagaaat aaccttgttg tacctcccgg
     2881 agcatggggt gattggatta atggcggcgg atggttagtc ataaacgggt atcatgtgga
     2941 tttaatttta cgtgatatta aacgtgtgga acaaataatg aaagatacag agcacggaat
     3001 tgttactgcc aattatcaga ctgggcatcc ccatggttat attagtgcaa tgtatcgagg
     3061 agaattagcg attagcaaaa tactatatgc taagaatgaa agcttatgcg aattaaaaaa
     3121 acaggcagaa acttatccca atgctttgca gaaaagttta gttaactttt ttatgtttga
     3181 agcagggttc tctttaatgt ttgtaaaagc aaattcggga acagacgata aatattatat
     3241 tgcgggtcat gtttttcgta tagtttcatg tttaaatcaa gtgttatttg catgtaataa
     3301 tgcttattgt atcaacgaaa agaaagctat aaaactgctt gaaacttttg aacataaacc
     3361 tgaaaaatat accgagaagg taaatcatat ttttgaagta ctcggtatct cactttttga
     3421 atgctacgac atgaccgaga agctttataa tgaagtgaat gaaattgtat cggagataaa
     3481 taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt
     3541 ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg
     3601 agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg
     3661 gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg
     3721 atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat
     3781 atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa
     3841 gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga
     3901 tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg
     3961 gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt
     4021 attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata
     4081 gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg
     4141 tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac
     4201 gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac
     4261 gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga
     4321 acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc
     4381 taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag aaccgtttat
     4441 atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa agccggagga
     4501 tatggaatta ttcccacctg aagaaaaggg att
//
LOCUS       DACEMO010000177         4514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEMO010000177 DACEMO010000000
VERSION     DACEMO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.183"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            62..1093
                     /locus_tag="IW728_002332"
     CDS             62..1093
                     /locus_tag="IW728_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1025670.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            1090..1857
                     /locus_tag="IW728_002333"
     CDS             1090..1857
                     /locus_tag="IW728_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1025671.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1957..3525
                     /gene="liaX"
                     /locus_tag="IW728_002334"
     CDS             1957..3525
                     /gene="liaX"
                     /locus_tag="IW728_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1025672.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3704..4009
                     /locus_tag="IW728_002335"
     CDS             3704..4009
                     /locus_tag="IW728_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1025673.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            4009..4359
                     /locus_tag="IW728_002336"
     CDS             4009..4359
                     /locus_tag="IW728_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1025674.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         1506 a          738 c          918 g         1352 t
ORIGIN      
        1 aaagggtttg tgacaaaaca tgtcacaaac ccttttcatc atttcataga aaggataaag
       61 aatgcaaaaa aaaattagca tcatcactat attttttatt tgtgtgctta cttcatgggt
      121 tattcagcat caagccgctt tatatgaagt acctgtcatc gagatagaaa aagtacgaat
      181 aaaagaggat catcaattga ttacaggaaa actgatgaac tcggatcaaa aaggagaatc
      241 gatttattta tcggttcctt atcaaaaaaa tcaaatagaa acagcaaata taaaagcgag
      301 acaaatgtat ctagtccaaa tgcatggaga tgaatggcag ctagtagcaa aaaaaaatga
      361 tggctggctt ttcttcttca caagtatctt cattggcgcc atgttgctta ttggcggaaa
      421 acagggtcta tttgctttag gaagtctcat catcaatatc gttttacttt tacttttgtt
      481 gctcatcttt actaaaacag aagggatttc tctattaaat aaatctatcc tgttcgtcgt
      541 tatagggatg attattagca tattggcttt agatggatgg aatagaagca gtataatcaa
      601 aatttcagct gcagttacga gtgttttttt agcatttttt atttgtcttt tgacaatgaa
      661 tcattggaaa gaccaaggac tgcgctatga ggaactgaat tatctgacac gaccgtatcg
      721 ttctgttttt ctttctagcg tactgattgg aacagctgga ggaacactcg ataccgttat
      781 cacagtcatt gcgacattag aagaattgac taagcgacaa ccagaaataa cagcgaaaca
      841 gttatggctt tcaggaaggc aagtgggtcg agatgtgatt ggctcgatga ccaatatcct
      901 tttgttcgtc tatatcagtg ggtcagttcc atcgcttctt ttatatttag gaaatcaatg
      961 gtctttcaaa gaaacgatag aacagcattt atcattggaa tttttacgcg tcattgctgg
     1021 cagcttagga atagtattat cgatccccat ctcggtcctc ttttttctga ctgtaaggag
     1081 gctgaaacga tgaatgtact tattatactt gttgggattt tagcaatcag cgtgctactt
     1141 attggcggta cgcaaggtat gtatattctt ttaggcttat tcatcaattt agggatcttt
     1201 ttcctgttgt tattcggtta tcatcagaag tggccgatac tggcattgtc ggtcatagga
     1261 tttttattga ttgctgtggt tatcttattt tttatcaacg gttataatct aaaaatgcgt
     1321 gcagcttttg tgagtatact gatcttttta ttttgtttcc tactattgct tcctatcact
     1381 gattttttag cgatccaagg ttttacatcg gtagaactag aagaattatc tggcttagat
     1441 aagactttag cgattgattt tagattactt acgagatcgt tgcttctgat tagtcttagt
     1501 ggtgctgttt tagatgcgag tgtagcaatt tctagtggga cattcgaagt atatcaggca
     1561 aatccgcaat tatcttttta ccaattgcgc cattctagtt tcgctattgc taaaaaaata
     1621 ttggcgtcaa cagtaatgac acttcttttt gcattcatgg gaagttcttt agcgttgatc
     1681 ctctggttta ttgatttaga cattcctttt cagcaagtga tcaatgaaaa atcatttgtt
     1741 ctagaatata cgatggctat tcttaccaca ttgtccgcac taattgttct tcctttaacc
     1801 tgcgtgacta cagcatatct ctttacaaaa aagaaagaac aactcaagat ggagtaaaag
     1861 ttacaactta agtcctatga catttttact gaatcagtgc ataatatgga tgtaaggaaa
     1921 agcttgcaaa caattttgct taaggaggca atttccatga aagaaagaga acgtatttta
     1981 gatttagtga aaaaaggaat cctttcgact gaagaaggct tagatttatt agaaagcatg
     2041 gcaactgaaa aagacgaaaa gcaaatcaaa aaagaagcag acaaggtaac tgctagtcat
     2101 aaagaaaaag ataaggcaag tcaattgatc gataaacttg aaaacggcga agaagaaatt
     2161 tctgagccag ttgatccaaa ggaaagagaa agacaagatc aagaaaactt agaaaagatc
     2221 ttagatgaac tagcaactga agcgaataaa acttcggcgc gtctagatga agtaaatgct
     2281 caattcgctg acaataaggc agcacgcaat gagaaacagg aagcattgat gcagctgaat
     2341 acaaaagaag aattaggcga gttgacagaa gaagaattag cacagcgtca ggcgttagag
     2401 gcagaaatca aagagttaga agctagtggc gatacactgt tagaagaaca attgaaatta
     2461 gaagcagaat taaaagatat ccgtaagaat caatggtctg agaaaaaaga aacgtttact
     2521 gataagtttg aattgccaga tgattggaaa gatcaagcaa cagatacact gaatcaagta
     2581 ggcgaaaaaa tgtcagaagc tggatcacag ttaggcaaat tcttgaagaa aacattccaa
     2641 actgtttcag aaacagtgaa tgacaacatg gaatggaaag atgtcagctt acgtgtccct
     2701 gggattgcga caacgaaatt tgagcatgaa ttttactatg aagctcctgc agcaagtatc
     2761 cttgatatca aggcagcaaa cggaaacgtg acgttaaaaa cgtgggacag caacgatgtg
     2821 aaagttgaag ctaagatcaa actttacgga aaaatgggtg ctgaaccatt tgaagcattc
     2881 tctgaacgga gccagattga ggttaatgaa gatcatatct ccttccaaat tccaaataaa
     2941 cgtgtgcgtg cagaccttgt tttctactta cctaaacgag tatacgacca cgcagctatt
     3001 aaacttctaa atggcaacat catgattgaa acattggaag ctaaagatat ttatacaaaa
     3061 tctacgaatg gtaacatcat cgtggaccaa ttaactgcaa cgatgctgga agtagaaggt
     3121 gtaaacggca atatcgatat tcgaaacggg aacatcttag attctattat cgaaacagta
     3181 aatggtacag tgacatttgg tgcgacagca gaaaatcttt ctgtgtcttt agtaaatggt
     3241 gatgtacgtc ttacgattaa agaagataat ttgaaaaaag tagaagcaag ttcagtgaac
     3301 ggaaatgtca aagtcgcttt accagatggc atcggtttgg aaggacatgc taaaacaagt
     3361 cttggaagta tcaatagccg tctgtccaat tacgaagtag ttcgtgagaa aaaagaacgg
     3421 acaaaccaaa tgttgcaatt ccgtcgtgtt tcagatgatg agatcgcgca agtgcaactt
     3481 tcaacgacaa caggaagtat ctatttaaaa gatacagata aataaagagg ttgtgaaaga
     3541 gatgttcagc cttgagcaat aagaagcgat tttgtaaaat agcttcccct attttacaaa
     3601 attgtgcctt attgccgata ggctatctct tgaacaccga ttaagaagaa ggttgtcaca
     3661 acgattttct tcatttaaaa tagtagaaca gaggtgagac atgatgaaaa aaaagttgac
     3721 aaaatccaat aaaaatgttg ttttgacagg tactttagca gggattgggg aatatatcgg
     3781 gattgatccc actgttatcc gtgtgcttta tgtattccta agcttagttg cttttggaag
     3841 tccaatcatt ttatacattt tattagcttt gattatccca tctgcgcgtt ctgcacgaac
     3901 aaactatgga cataacaatg catattatga aaaaaacaat tatcgtgaac aaacaaaaaa
     3961 acgcaaagaa gcggaaaaaa tcgatgatga cgaatggagt gacttttaat gtcgtacttt
     4021 cagcgtattt tggttaatac gttaacattc atatcgttgg ctgttctttt tcctgcgatg
     4081 gtccatgtga gcagtatttg gacagccgtc cttgccagct tcgtattatc tgtgttgaac
     4141 atgtttgtca aaccgattct gacgatcctg tccctgccct ttacattagt cacattggga
     4201 ttgtttacgt ttgttatcaa tgcagcgatt cttaaaatga cttccttttt tgtaggagca
     4261 gaacatttta gtttttcttc atttggtgca gcgttattcg tagctatctt gatgtcactt
     4321 gtcaatgtaa tcgttagtga acataacagc agaaagtaaa aaaagctgtg ccgagatctc
     4381 ggcgcagctt ttttgtttat cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt
     4441 tatggtacac tcaaattgta tttccgacga aagaaaacaa aggagtgtaa ccataaatga
     4501 cctacaaaca tctt
//
LOCUS       DACEMO010000178         4466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEMO010000178 DACEMO010000000
VERSION     DACEMO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.184"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            132..1433
                     /locus_tag="IW728_002337"
     CDS             132..1433
                     /locus_tag="IW728_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1025675.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1460..1720
                     /locus_tag="IW728_002338"
     CDS             1460..1720
                     /locus_tag="IW728_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1025676.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2719..3954)
                     /locus_tag="IW728_002339"
     CDS             complement(2719..3954)
                     /locus_tag="IW728_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1025677.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1316 a          700 c          904 g         1546 t
ORIGIN      
        1 cttaaaaata aatttgatta tttaagagaa tatattgaaa taaaatttct taattatata
       61 attagttatg aacattcttt gcgttgtcat gcaatgaatg gttcatattt tattgaaaag
      121 gagtggtttt agtggaaaag ctgatgaaat gggtcgaaga gaaacttgtc ccaccaatgg
      181 caaaaattgg tacacagcgt catttattag caattcgtaa tggggttgtt tcaacattgt
      241 cattgatttt gattgggaca ttttttatgg tctttatcaa tttaccgttt cctggatgga
      301 atgaatttat tgccccttat tcggcaacga ttgttttacc ctttaggatg acgatgggat
      361 tgatggctat ttatgcaact tttgttatgg gttcggatct agctaaatca tatggattag
      421 atagtgtgac aggtggtata ttatctttag gaacgttctt tatgttacaa gttcctgtaa
      481 acgttttaac tcctgaagag gcacctcttg gctgggtctt acctatgagt tctttgggag
      541 catctgggat gtttgcgggg atcttatcta tgatttttgc agtggaagta tatcgtttct
      601 ttaaaaatcg aaatattact atcaaaatgc cagaacaagt gccacctgct gttgcccgta
      661 gttttgaggc attgattcca ggtgcagtca ttttaacaat tacttggctg attcgaagcg
      721 tcgcgggctt tgatgtaaac gctgccttat tatcgttatt cgagccttta accaatattt
      781 tagggaataa tttgcttggt gttttactac caatgttttt gatccatttg ttgtggtcat
      841 ttggtatcca tggcatgagt attgtagggg ctgttgttcg tccaatgtgg ctgatcatgt
      901 tggatgagaa tgcaaaggca ttggcagatg gtacagctgc cacccaatta ccctatatcg
      961 ctcctgaaca gttttatcaa tggacggtaa caattggtgg tgcaggagca acgatcgtgg
     1021 tatctgtttt gtttatattc ttctgtaaat caaaattctt gaaagaggtt ggtcgttttt
     1081 ctattattcc tggtatcttc aatatcaatg agcccataat ttttggagct cctatgatgt
     1141 tgaatcctta catgttcatc ccgttcaact tagttccttt agttttgact attatcagtt
     1201 attttgcagt caaattagaa atggtcaata gatttactgt tttgccaaca tggacattac
     1261 cagctccaat cggcggttat ctttcagcag gaaatgactg gcgtgttgtt atcttgatca
     1321 tgatcaacac attgatagca tttattatct attatccatt tgtaaaagct tatgataaaa
     1381 agatgctgtc agatgaacaa agtcgtggag aaaacgaagt ccagactgtc taagtttggt
     1441 ttttagaagg tgggaaacaa tgtattggct gattttattt tttgttttta tctttttatt
     1501 aacggctagt caccttatat tgaatatgct agctacatat catattcaaa tcaatcgatg
     1561 gatttgggca ctggcatcat ttttgatcgt gattcttcca aagatcattg taccgcatat
     1621 gaatgttttg ttttcatggg ggacatatgt actttgtgga atttttgcta tcaattttat
     1681 gatcgagcag catagatggt ttgtcacatc gaagctttaa atgttctaaa agtggttgta
     1741 acagaagtgg ttagcttcaa gaaataagaa gaaatttacg aaaattgctt cttaaatttt
     1801 tgtgaaatgt tggcttattt ttcgtttggt tgaacttatt accgcagatg caagcttttg
     1861 accaccgttt attcggaata tgggactgtg acaaggcttt tgtcacagcc cttttttaca
     1921 taaagattat tattgtttta actgttttgg cagaaagaag gaggaaaaaa tggaaatttt
     1981 atcagcaatg gatttagaat tagtagatct actaccaaag tgatcaagtc aaatatagat
     2041 ttgggagctt acaaccatat agtaggggct gcagtatggt gtatgaatgg taatatgtat
     2101 gatgtgagtg tttattcaaa tcatgaaacg tgtatagaaa taattgtgct gggaatagca
     2161 atcagtcatg gtgaacgtga cttttagtgt atcgttgctg acgacataga acattctgac
     2221 atgatctact caatatgtgg ggattgctgg cagtttatat aggagtatgc atcggattgc
     2281 gagatcattg tctcaacaga agagggaggt tgattatcaa agataaagtg gtaaatccta
     2341 catttgattt agtaattgtt gaggggacta gcggcacaaa tctctacaaa gcgatgcttc
     2401 tagatctata tgacacattt taagaaaatg tatttgccta attattatgg tctttctttt
     2461 gaagaaacgt tgagatgatc caaccagaga aaggtgtcaa agaatttgac taagaataaa
     2521 tggcagacgc tacctatgct catttggata gcgtcagatc ttttatttta aggatgattt
     2581 tgtgtttttt agacaccgta atttaagtaa ctctaacttt tcaatccact atttactttt
     2641 tttgtatttt cactgtctgg gtctcgaata gcgaaattac tgatgagtct ctattaaaaa
     2701 agctttttct acttgaaact atattgggtg gttgctcagg aaggtttcga tttcttgaat
     2761 tgcttgttgt ggaccattta ttggaaaaac ggcttgcagc gtgcgtgcct gctgacgaat
     2821 cttgggtgaa gaaacttcgc gcagagcaat ctgcagtttt ctttcagtca ctggttttgt
     2881 gagggcgatt gcgtttccgt attttacaca atagtcaata ttacagcttt gctcaaattg
     2941 catccccaca ccgataaacg gtttgccgga ttcacaggca gtttggacgg tcccttcccc
     3001 gccgtggata aaagaaaaat caatgaattc atgcaaaaga tgactagaca gatattctgt
     3061 tattaaaaga tcttctgagt cagattgaat gtcatcaagc agatagtcaa taggtgcaat
     3121 aaccgctaga tccggttgtt tttgtaagaa gaacaaaatt ttgcgtaaca tacgcggatt
     3181 gcctgaacta cccattgcga aatagataat acgttttctt tcttgttgct ttcgctgtat
     3241 aaaagttagc acttcttcag gcaaggaagt actgagctta gcaaaaattg gaccaacgat
     3301 tcgataattt tccggcagct gttcttcttc caaaaatagt ttaggcgttg tgatgagatt
     3361 tagatcagca tccatcattt gtaaacttgt ttttggtaaa ggaagcattt ctttttgtgc
     3421 agcgtgtttg aaggcattgg ggatccatcg ggtatttact atacgctttt ttgtccagtg
     3481 ccaaagggga ttgcttaaag ggccgaagaa acgtaactcg tggggcatca gcgcatgctc
     3541 tgattgaaaa aaaggttcac tcaaagcata gggtttaaca tatactaaag gaatcttttt
     3601 attgcgggcc gataaaaaaa ttgtcacatt cgaacctgtc acaatcagtc gaggctcaaa
     3661 cttagcaatc agactctgtt cactagtgat acgagaggca accatttctt tggtaaaagg
     3721 gtttttaagt gtacgcaact ggtcaaattt tatgatttgt tcttcttgtt ttttcgtaag
     3781 gatcggcgct agtaaatgat actcaaagcc actttctgtg atcaaaggag caaaagtttt
     3841 agaaaagcca aagaatacac agtaataacg gtctttcata gctttggcaa cctcaatcat
     3901 tcgtgttgtc tcagctaaat tgaatactgc aggagcaaaa agaattgtct tcatgaaatc
     3961 ctccttattg gaatgagtct atcccttcgt ttcttactag tatactaaaa aacacagcgt
     4021 tcgagtagcc tatgtatatt ttgaatatta aactgtctct cgctgttttc acaacttcta
     4081 aatttaaatt ttgtaatcaa gttgaaactc caattttcaa aaaaacacgc aacaattttt
     4141 tgatcgattt atatgttgtg ccagataaat acgagaagtt gtgtcattaa aaataaacat
     4201 gagaacataa aaaaataaaa taattatatt aattgatagg tgtgctgtcg tcattataca
     4261 cacgtaagaa agaaatagtt atgtttaaaa atattcttag actttctcgc catgacaatt
     4321 ttcactatgt gttataactg taaacgataa ttattcagat aacacaaaaa gaaatcaagg
     4381 agcagatacg ccagttgttt aatttgaata ttattttaaa attaaaattt ttattctgtt
     4441 ctaaacattt ttggttgttt agaact
//
LOCUS       DACEMO010000179         4389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEMO010000179 DACEMO010000000
VERSION     DACEMO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.185"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            205..435
                     /locus_tag="IW728_002340"
     CDS             205..435
                     /locus_tag="IW728_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1025678.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            532..1833
                     /gene="murA"
                     /locus_tag="IW728_002341"
     CDS             532..1833
                     /gene="murA"
                     /locus_tag="IW728_002341"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1025679.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1846..2022
                     /locus_tag="IW728_002342"
     CDS             1846..2022
                     /locus_tag="IW728_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1025680.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2157..2453
                     /gene="yidD"
                     /locus_tag="IW728_002343"
     CDS             2157..2453
                     /gene="yidD"
                     /locus_tag="IW728_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1025681.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2517..2900
                     /locus_tag="IW728_002344"
     CDS             2517..2900
                     /locus_tag="IW728_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1025682.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            3069..3953
                     /locus_tag="IW728_002345"
     CDS             3069..3953
                     /locus_tag="IW728_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1025683.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4070..4303
                     /locus_tag="IW728_002346"
     CDS             4070..4303
                     /locus_tag="IW728_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1025684.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         1492 a          669 c          959 g         1269 t
ORIGIN      
        1 tgattgaaca ccgtttattt ggaataaggg actgcgacaa gacttttgtc tcagtccctt
       61 tttttgcata tttttagcat ttatttccta aaaaatgtaa ggggattcct atatttttaa
      121 gtatacttgt ttttcctttt atacatttta tggtattatc attttattag ttattataaa
      181 acaatcgtta gaaaggacgt cattatgcaa gtatacggaa ttgatgcggt gcttcgaatc
      241 gtgagtcatt tggcgtttat ctacttagcc ttctggtctt tacgttcgtt acgcattgaa
      301 acttttttca gaccaatgca taatacgcaa attagaatgg caatcgtact tttttcaatt
      361 tttttagggt acactgcaag taattttttc ttagaagtta tcgctttatg ccgaaatctt
      421 ttcgttagcc tgtaaatttt aaaaaaaggt actattttaa aagcaaggat gttataatag
      481 aaaagatttt gagtaagtgt atgctcattg gaattttttg gagggaacaa catggaagag
      541 atcatcgtaa gaggtggcaa tcaattaaat ggaacagtaa gaattgaagg agctaaaaat
      601 gctgttttgc ctattttagc tgctagtttg ttagcagaag aaggtattac gacattagac
      661 aacgttccga ttctttctga tgtcttcact atgaatcaag tgattcgtca cttaaacgta
      721 gatgtagatt ttgatgaaca aaagaatcaa gtaacaatcg acgcttcaag acaattagaa
      781 atcgaagcgc cttatgagta tgttagtcaa atgcgtgctt caatcgttgt gatgggtccg
      841 ctacttgcca gaaatggaca tgcaaaagta gctatgcctg gtggatgtgc gattggtaaa
      901 cgaccaatcg atttgcattt aaaaggattt caagctttag gagcaaaaat tattcaaaaa
      961 aacggctata tcgaagcaat tgcggatgaa ttgatcggta acacgattta tttagatttc
     1021 cctagtgtgg gtgctactca aaatatcatg atggctgccg taaaagcaaa aggaacaacc
     1081 atcattgaaa acgtagctcg cgaaccagaa atcgttgatt tagcaaacat tttgaataaa
     1141 atgggcgctc aagtctatgg agctggtact gaaacaatgc gtattgaagg cgtggatcat
     1201 ttacatgcag tcaaccactc aatcgtacaa gatcgtatcg aagcaggtac ctttatggtt
     1261 gctgctgcaa tgacacaagg caatgtgctg attgcagatg ctatctctga acataactgc
     1321 ccattgattt ctaaattgat cgaaatgggt gcagaaatca ttgaagaaga aggcggagtc
     1381 cgtgtcatcg gaccaaaaca tatcttacct acagatgtaa aaacgatgcc tcatcctggt
     1441 ttccctacag atatgcaagc tcaaatgaca gctatccaat tagttgcaga aggtacaagt
     1501 gtggtgactg aaacagtgtt tgaaaatcgt ttccaacatt tagaagagat gcgccgtatg
     1561 aacgctcatg tcaaaatcga cggaaatgta gcaatcatgg atggaaacca tgaattgcaa
     1621 ggtgcagaag tatatgcaac agacttgcgg gcagcagcag cattagtttt agctggcttg
     1681 aaagcaaatg gaatcacacg tgtgcgtaac ttgaattatc tagaccgtgg atattataat
     1741 ttccatatca aactacagca attaggtgca gacgtcgaac gtgtagacat ggatcaaact
     1801 tctgctgaaa aaacagcaca aacgattgct taaggaagtg gctgaatgag taaccaacga
     1861 tatatcttat taactttgat aaagatttta gtggtgatct tattgcttat ccttcttttt
     1921 gtggccggaa cgatgatcgg atatggtgtc atcggtggcg gcaatccatt taaagtattc
     1981 cagccaagtt tgtggattca tattcgagac ttttttcatt gataaataag aatcacagtg
     2041 gttctagtcg atatgaagtg agccatgaag cagaacatga taaaagaaaa cagaagggaa
     2101 cgttgacgtt agtcgcgatc ccttttttta gtaaaagaaa agaaggaatc taattgatga
     2161 agaagatatt gatttattta gttagaggat atcagcgctt tatttcgcct gcatttccac
     2221 ctagctgccg ttattaccca acctgttcca attatatgat aaaagcaatt caaacccatg
     2281 gtgcgctaaa agggacggta atggggactg cacggatctt acgctgtcat ccttttgtca
     2341 aaggcggtat cgattatgta ccattgacat tccgattaac gcgaaataag aaagataaaa
     2401 aagaaccaga ttataaaaag gttgtgaaaa atacgggtag cactgagcga taaagaaaaa
     2461 gaggaaattt attgtcaaag taacaactcc gcaactactt atatggggga caaaaaatga
     2521 tcgaagaact aaacaagaaa gatttaccat ggtccttgtt attagatgca gatcctgata
     2581 aagaaaaagt tcaagtatat gtttccagag ggagtggctg gatatggaaa gaaaaagaaa
     2641 aaacaatagg tgtactgatc tatgtggcaa gggaaaaaga atacgaaatc gtcaatgtag
     2701 cagtaacccc aacgtatcaa ggaaagggga ttggcggaaa attacttgag acagcttttc
     2761 agaagctttc acagctagct tcaaaccaaa ctcggatcat tatacgtact ggcactacta
     2821 gtagcgctgc gttacacctc tatcaaaaaa tgggctatgt ggagattggt cgagtcaaag
     2881 actattttat ccatcattaa tctgagccca tttttgaaaa tggagatcaa ttaagggatc
     2941 aagtgatttt agcaattcac ttacctaaaa atagctttaa ctaataaact ttcaattcga
     3001 gaaaatgctt tcaaatgcta aatagataca ttacaatagc tttagcaaac aaaaaggaga
     3061 atatttttat gggaatatta gtttttgatt ttggtggatc ggctgtcaaa tatggttgtt
     3121 gggatggcaa agagattaaa ggtaagggac aatttgcaac gcctgaatct tgggaagaaa
     3181 tgaaaacgca attatttcaa gtttataaaa aaatactcgt ttcttttgaa gtcgaaggaa
     3241 ttggaatcag ctctcctgga gtagtgaata atgagaaaca ggtgattgag ggaatcagtg
     3301 cgattcctta tattcatgga tttaatattt tccgagattt ggaaactctg tttcaactcc
     3361 cagtaacgat tgaaaatgac gccaactgtg ccggaatggc tgaattttat gaaggtgctg
     3421 cccgtgatta tcaagatgtt gcttttgttg tggcaggaac tgggattggc ggggcgttgt
     3481 tcacgaaagg acaaataaat aaaggggcac atctttacgg tggagaattt gggttgatgt
     3541 tcttagatgg cgataagaca ttcagtaaac ttggtacagc ggtacagatg gcttggcgtt
     3601 attgtgaacg caaaggtctc gataaaaatg cttataccgg aaaagatgtc tttgaattag
     3661 cagaaactgg agatgcaata gcaaaagaag aagttgagag tttttatacg tatcttacaa
     3721 agggattatt cagtattcaa ttttcgtttg atccagaagt gatcgtatta ggcggcgggg
     3781 tttctgcaaa agaagggctc attgatgaga tcaaaagccg aatgaagaag ctgacagaac
     3841 aattcgatct gcatgatttc gaaccgcaga ttttattatg tgagtacaga aacgatgcta
     3901 atcttgtagg tgcagcagca aactatctcg ctgtacgtca aggagagaat taagagcttt
     3961 agagaaggtg cagcttgcat aaaagaagag aatgaaatga ataggagatt tttacctaag
     4021 gaatgtttta tttgctgcag tatgataaaa tatctgagag gtgaaaatga tgtcaaaaaa
     4081 aaataaagat attgaagtgc gtatagaaga agtgaaaaaa gatatcaaag gtaagaatta
     4141 tgaagtcact cagttattta tagggaaaaa aatgattggt gagatcttag cctatgggcc
     4201 aaaagaatat gaaattttct ttggtgaaga agattttgga aaagaaaaaa gtcttgaaaa
     4261 tgcgatagaa acagtgattc gacactggaa tctgcacgaa taaaaaaata tcaaaaaaac
     4321 tttcaataaa gggttgcaaa aatctggaaa attaggtatt ataactaagt caggttgata
     4381 caaaccttt
//
LOCUS       DACEMO010000180         4369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEMO010000180 DACEMO010000000
VERSION     DACEMO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.186"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            302..1282
                     /locus_tag="IW728_002347"
     CDS             302..1282
                     /locus_tag="IW728_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1025685.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            1404..2822
                     /locus_tag="IW728_002348"
     CDS             1404..2822
                     /locus_tag="IW728_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ1025686.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            2893..4362
                     /locus_tag="IW728_002349"
     CDS             2893..4362
                     /locus_tag="IW728_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1025687.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
BASE COUNT         1466 a          639 c          908 g         1356 t
ORIGIN      
        1 taccggtggc gaggcacctt aattatttga atgagcttct gccttagaga atcagattcg
       61 aggaggctcg ttgtcaaggg cagtcggaac accagcagca agcttacgca ataccttata
      121 atttttgtca aaaaaagtgt tgccattcca catatatctt taaacagcta ttgccagata
      181 aaaataaatg atattatata tataaatact tatccgaaaa ggggaatgag agagtcaagg
      241 ttcttggaat gtcgaatagt cgagtaggta gattttctat ctacaacctt gttgtttaaa
      301 aatgaaaaaa attggaatta gagaagttgc gaaagaggca aatgtttctc ctacgactgt
      361 gtcgagggta ttgaatgatc gtggatattt gagtgaggaa acaaaaagaa aagtttttga
      421 tgccatgaaa aaattagaat actatcctaa tgaactggcg agagcacttt ttaaaaacga
      481 aacatatacc gtaggtttga tttttcctac aattactaat ccatttcacg ctgagttaat
      541 tcaagatatc gagttcgagc tttccagcag gggatataaa gtgttgctgt gcaatagctt
      601 aaacaatcca acaaaagaaa aagagtactt gacgatgttg agaaagaatc aggttgatgg
      661 aattatagta ggaacgcaca ataaagatat tgcggattat gatattccag ggctacctat
      721 tgtagcgatt gatagaagtc ttggtgaaaa tacagttaca gtatcttgtg ataattacag
      781 gggtggggaa ttagctactc aactacttgt aaatcatggc tgtcagaata tactgtgtat
      841 tcggggagat agtaagataa agctacccgc gaatagtagg actttagcat ataaagatgt
      901 tttgaataaa ttcatgctgg aaccgttagt cctagaagta ccatttgtaa aagaaatcaa
      961 tgaaaaaaat gaaataatta agacatatct ggaaactcat ccaaatattg acggggtttt
     1021 tgcaggagat gatttattgg cttctttggc gattaacaac ttaggaagtt cgggcaaaag
     1081 agttccaaaa gaggttaaag tgattggatt tgatggagct caacaaacat tgatttataa
     1141 tccagaatta tcaactattc aacagcctat agatcaaata tctaaagtag cagtaaaaaa
     1201 attattgaat atgattaagg gcgaaaagga gacggatgaa ctttcacttc cagtaaagct
     1261 cataaaaaaa aatacagcat aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat
     1321 atttttatga aacgggttga catatgaaac gggttgacat atattagttg tgaagataaa
     1381 aaccttttca ctggaggaag aaaatggaga aattattaat taaaagcaat aatcatcaac
     1441 tttactacca acctaaggat gtttgggtcg gagatgttat gccttacggg aaagaaggaa
     1501 aattctttat ttatcatcaa cgtgatacta gaaatccagt tccttttgga gaaccgtttg
     1561 gctgggcttt agcaactacg aaagattttg ttcattttga agactttggg gaatctcttt
     1621 taagagggga tgacaatgag agagaccagt ttatctacgc tggttctgta tttgaggcaa
     1681 atggaaaggt tcacgcattt tatacagggt ataatcgtga attcttagcg cagggaaaga
     1741 catctcaagt gttgcttcat gctgagagtg atgattttgt tcattgggaa aaatcagaaa
     1801 cagcattgga gttagaacca caagaagggt atgatattcg aaactggaga gatccatttg
     1861 tcgtttggga tgatgaaaaa gaagaatatt tgctaatttt aggggcgcga aagggtcagg
     1921 ataaacatct acaaaccgga cgtttagtgg aatttacatc aaaagattta actaattggg
     1981 aattcaaagg tgatttttgg gctccgggat tgtataccat gtttgaaatg ccagaacttt
     2041 tcaagattgg tgactggtgg tatcttgtct attcagaata ctcagatgga aataaaatac
     2101 tctatcgaat gagtcaaagt ttggaaggtc cttggattaa gcctaaagat gaagcttttg
     2161 atggacgggc ttattatgca ggtagaacag cttatgatgg aaaacgtcga gtcctatttg
     2221 gatgggtacc aacaaaagat caagaaaatg ataaaaacaa ttatttatgg ggaggtactt
     2281 tggttcctca tgaagtctat caaaaagagg atttttcact tggggtaaaa cccatagacg
     2341 aagtgtggaa tgcattcata aatttacatg atattgccga tgtagagata gaaacagcag
     2401 acacaagaga agaagtaacg cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa
     2461 caatttcatt tgatgagaat cttagtgact tttcactgag attatataaa aaccttgaga
     2521 cagatgaatc ttacgaatat cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa
     2581 acccttgtta cccttggttt caatatatgg ataaaggact tattagacct atatctttaa
     2641 agcctaacca atcttataaa ctaaaagtga ttgttgatga caatataatg acactttata
     2701 ttgatggaat cgctcttaac gctcgtgtac atggttgttt tggtcaagca attactatta
     2761 cagcaacaaa tggatcaatg actgcaataa ataccaagtt ttcaaatgaa ataaagaaat
     2821 aataaacgtc aatcatgtgg caggtctgaa aatcaaacat gacctgtcac atttaaaagg
     2881 gaggataaat gaatggacta caaaaaaatt gccgaagatg tattggaaaa cgttggagga
     2941 aaagagaata tctcaagcgc tacacactgc attactcgtt tacgtttaat tcttaaagac
     3001 aaaagcaaag cgaatatgga aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc
     3061 aatagtggac agttacaagt aattttcgga acaggtataa ttgaaaaggt ttacaaagag
     3121 ttcgttgaat tgacaggagc taaggaagta tccgtttctg aaatgaaaga cgaaggagtc
     3181 aataattcag gaaatagact tcaacgagct tttaaagttt tctcggatat ctttattccg
     3241 atcattccag cttttattgg ggcagcaatg gttttaggaa ttcgttcgtt gattaccact
     3301 cctggaatgt ttggaatgga tggctcattc gctgatcaca atgtattcct aaatgattta
     3361 tccaaatttc tgggtattat tgcaaccaca tttgattatt taccagtttt agttatgtat
     3421 tctgcaacaa aacgttttgg cggaaaccct attttaggat tacttgtagg atttgttatg
     3481 attcatccag gactagccaa tcggaatgat ttcgtcatgg gaactataaa gcctgattat
     3541 tggcatttat ttggattaag cattccagca gttgcgtttc aagggggtgt ttttccagca
     3601 attttgactt cttggttttt agcaaaagtt gagaaaatta caaataaata ttctcctcaa
     3661 gtacttgctt acattttagt cccaactgtg acaattgttc ttgccaactt tgcactattt
     3721 ttagtgtttg gtccattggg gaattttatc ggtaatgttt taggtggaat cattgatttc
     3781 ctatatatga agatgggtgc ctttggtgct tttgtttttg caggcgcgtt acaaccatta
     3841 gttgtaacag gaacacaaca tgctattcaa ggtattgagg caaacttagt cgcaacaact
     3901 ggattcaatt atatccaacc tatttggtca gtttcaatta ttgcccaagg tggcggttgt
     3961 attggtatgt atttactctt taagaaaaaa tctaaagata gagatattgc gatttcaagc
     4021 tttattccaa cactggtagg tattactgaa ccggctatct ttgctgttaa tttgaaatat
     4081 tcaatcgttc cttttgtttg ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa
     4141 atttttgatg taaaagccat tggacaagga ttgactgtat tgccaggtct gactattgct
     4201 aatccagttc ttccttatat atttggtaat ctcatagcat tcattttacc aatcattttt
     4261 attctcgctt atgataaata taaagtgttt atccctgaag ctaaacaccg taaatttgca
     4321 gaaaaaactg actcaaaaaa attgccagtt caagtaaagt gagatgaga
//
LOCUS       DACEMO010000181         4361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEMO010000181 DACEMO010000000
VERSION     DACEMO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.187"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..2378
                     /locus_tag="IW728_002350"
     CDS             6..2378
                     /locus_tag="IW728_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025688.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2439..3899
                     /locus_tag="IW728_002351"
     CDS             2439..3899
                     /locus_tag="IW728_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1025689.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3910..>4361
                     /locus_tag="IW728_002352"
     CDS             3910..>4361
                     /locus_tag="IW728_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1025690.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWALEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQ"
BASE COUNT         1678 a          705 c          943 g         1035 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg cttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaacg aacaaattga gaagatgttt gaacaccaga
      121 gaaagaccaa cggtcagcca atgaacgata agagaaatgg gcattcttgg tttaggcaac
      181 aagaacgttt agaaaacaaa gtgcatagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggctataac aagtacggcg
      301 gcctggatat gacaatcgaa aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gactttttca gccgctacga tccgaaaata ccaacggaaa ttggaaacat
      421 tagaacagct gaaagaaaga tcagaaaaag gaaaagaaaa tcttcttcct gaagtacaag
      481 caattattga cagcggacga gtgacgcaat ggaaaaaaaa tccaaccatt tattttttga
      541 aagactatcg aaaagttgct ttagaactga atgaaaacgg gggatttgag gaatcaaaaa
      601 aataccaagc cacgtcggaa gaggaaaaag cagctgttcg tgaattactt gaagaggctg
      661 cgccaactga ttctttgaag gagaaagaaa acgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga gatcgtaaca ttagaattga
      781 tcgatcaact caaaacgttg gataaccaat acgccgcaga agcaggttat tataaattct
      841 attttgaaga agtgaagaac ggagaagtag tcgatcagat ccgaatggat ctaggggatg
      901 gcgtcaacag taatcaaccc atctatttag agttagcaaa attgtgcgaa caagtacctg
      961 aaaaggaaca aaaacaaacg gtcaaaaatg aacctgtttc gaaagaacaa atgcagtcga
     1021 aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac ggcagaagag attaagcaag
     1081 aagtcaaatc cctttcgcaa caggcactgg atgcggtgaa aaagcatacc cattctcctg
     1141 aagacgtgaa agaacttttg aattttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat tgaggaacaa tttccaggtg caacggcttg tttaggtcgt gctgcgttga
     1261 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
     1321 cggtcaaagg attttatgag aaaggacgtg gctttgttcg agaaacagaa gctacaccag
     1381 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tactacacga
     1441 ttgaaaaagt gtttgatatc acacaaacac agttaaaacc tgaagactat ccgaaaatct
     1501 ttccgaaccg tgtctttgat tttcaattgg ataaaaaagg acagtcagaa cttcaagctg
     1561 ggattcaagc ggtagccaac aatattggaa tacaaattcg agatatgaca gaaagtgaag
     1621 tctatcaaag agaattagga caagcacggg gggcgtatgt tcgtaacgaa tttactggcg
     1681 aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagct acaagtattc
     1741 atgaattagc ccatgcaaga atgcacaaat tttcagaatt agataccgca actaaagaat
     1801 tgcaggcaga aatgacttct tatattgtgt gcaaacattt tggaatggac acttcggaga
     1861 aagctattcc ttatattgca gcatggacga agaatggtca gacgttggat gataaggaag
     1921 cggcggaacg tgggaaaatc atgaatgatg tcagccgtgt cgcaaatgaa ttcattcaaa
     1981 ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccaa ccagcagaag
     2041 tgaagcagga gcataaaaat actgtagaaa cggcagagaa taagaatatt gtgcccgttg
     2101 gttcactttg gatcgacaag aaagacggga aagtaatgga agaagatacg ggtcgaacgt
     2161 tacatttgaa ggatgcggcg tttattgaag atccgcagca taatcaagtg attttaaaag
     2221 gaaaaagtta tggaaaagag attcaaacgg cgttaccttc agaagaattt gcgctaggaa
     2281 atccattccg attttcactt gaaacagtgt cagaagtggc caaacgaaaa aatgaaatct
     2341 attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaaaaa agactatctg
     2401 aaaaacggat agtctttttt cttttgggag gtttgaaaat gaccgaaaaa aaaatgagtc
     2461 ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat ggaatggctg
     2521 tagtcaataa aggacgagac tatcgcttag aagaccatga ttcatttgtt tttgatcgaa
     2581 gaaaacaacg tttttattgg aacagtcaaa atattagtgg cgacattatt gaattagcta
     2641 aacttttttt catagacaaa gagatccaag actctaagca acaatttaaa gcagccacag
     2701 attttattct aaagaatgaa gacaaaacag aacgagtaga aaatcttcat tttgagacag
     2761 aaaagtacaa agatcacccc gttgattatc aaccactcac agaaaaagga aggaattatc
     2821 tgaaggaaga acgaaaacta ccagagtggc tgattgatta tgcagaaaaa gaagggttga
     2881 ttgccgaact aaagccaaaa catgaacggc aaaatttctt agttcgagac gatcgtttag
     2941 atcatgcggt cgccttttta tggaaagatc cacagacaaa tgagacagta ggggcgagtt
     3001 atcaaggaac gttcattgat tatgaacgtt ttggtgagag aggcacatat aagcatatag
     3061 ataaaaattc gacagcgaat catggcttta acctaaagat cggtgaccct aagcagttaa
     3121 aattttttga gagtagtatt gatttactta gctatgctgc actaaatcgt gatcagttaa
     3181 acgatacgtg gttagtttcg atggaagggt taaaacacca tgtgatcagt cattattttg
     3241 gtgaggcagt atctgaattg agaaagaaac aagcctttcc tcaatccatc gagatttgtg
     3301 tggataatga ccgtgcagga catattttct acgaaaaaga acaattaatg ggcgcagttg
     3361 atccatttac gaatcagaaa gtacgttgtg aacgaggaat cgctaatgat tggcaagtgc
     3421 caaaagaata caaagtcatt tatgaagaag tcgcaaaaga agaaaagatc acaccagaag
     3481 ctattatggc catacacaag acagaaaaca atctgcaatt aacgaaccaa ttggtttccg
     3541 ctcacaaagt aaatgcgtct tttggtcagc agctttctgt taacgattca atagaagcta
     3601 ttaatttgaa agatatttgt agagaagtcg caaaagaatt aaaagcttgt gaacgcgtgg
     3661 atggtacgta cgattttgat cgtttttatc aagaaaaagg ggatattaat gctcagattc
     3721 ttttctctta taaagcagaa caatactaca aaggctataa aaatcatgaa catgaatttg
     3781 tacctgaagt aaaaaaggat tggaatgatc aattgaagca tgaaatctac caacaagaga
     3841 tcagaaagca aaaacgtgcc atgttgtttc aacaaggtag acagcaagaa agggagtgaa
     3901 atcgtcatga tgaaaaccgt tattttagca gaaaaaccgt cacaagcgaa ggcttatgcc
     3961 gatagctttt ctaaagctac ccgaaaagat gggtattttg agatccaaga tcgattgttt
     4021 tcaggagaaa ctgtgatcac gtatggtttt ggtcatttag tcgagttaga ttcgcccgat
     4081 atgtacgatg aaaattggaa acaatgggca ttagagcatt tgcctatttt ccctacacat
     4141 tatcactatc atgttcctaa agataagaaa aagcaattca aggtggtgaa acagcaactt
     4201 caatcggccg acaccattat tattgcgaca gatagcgatc gtgaagggga gctgattgcc
     4261 tggtcaatca ttcaacaagc aggcgccaat caaggaaaaa cctttaaaag actttggatc
     4321 aactccttag aaaaagaagc catttatcag ggattccagc a
//
LOCUS       DACEMO010000182         4353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEMO010000182 DACEMO010000000
VERSION     DACEMO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.188"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..295
                     /locus_tag="IW728_002353"
     CDS             <1..295
                     /locus_tag="IW728_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025691.1"
                     /translation="LTVERINEIPDCLEKDHLFLIRKMLKDENLEPNDFIVVEYPATG
                     IYCCNHINNGQYFIIQEGKNKLVPYYTTCDMNEEGINNFPCTSIKESMKLIEM"
     gene            316..585
                     /locus_tag="IW728_002354"
     CDS             316..585
                     /locus_tag="IW728_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025692.1"
                     /translation="MQTFTQHELEIISGHKQIAGSNANKLSVIKSEVGEMLSMLESDL
                     GKQYIEDDEQRHYTLKNYQSVYDKLEKMSEDEFKQIDLSGIEELE"
     gene            821..1234
                     /locus_tag="IW728_002355"
     CDS             821..1234
                     /locus_tag="IW728_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1025693.1"
                     /translation="MEQKLAKTPENILQIAKTYDNDERNIQKKFRVSEREKFLIDYKM
                     SQANTRNFSRYARIMCLSGLMITTDFSDLKNLTTELGRIGNNINQLAHRANISNSVSK
                     EDFLELKINYDQLTKLINEELLFRMRRARDNQRFI"
     gene            1234..3528
                     /locus_tag="IW728_002356"
     CDS             1234..3528
                     /locus_tag="IW728_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1025694.1"
                     /translation="MAVTKIHQIKTTLNKAIGYITNPEKTNNGMLVSSYNCQSQFAEV
                     EMQMTREYAREVKGDYRKVGGSEVLAHHLIQSFSPEDKVTPELAHELGRQLIDELTEG
                     KFEYVIATHIDQEHIHNHIIFNSVSFYDLKKFRSQPYRTANKIKEISNRICAEHDLSL
                     SPKKEKLKDSYKSYSNRRNNTSFRSEIRKRLTMCLNESTSWEEFEEKADHLGVNIDRK
                     GKHISYALLSEGQQRRTRGDKLDDLETFTESNISQKLSANQSYINQLKEAIEETFFSS
                     DNLADFSFLLEQEHGITFKLNKQDQFVFHFNDVNDFKWNESLLPDSYQIKNLKDYFRE
                     SYEFTNDSSLNSIEERYREKEKSVIVQEDTPIQLQEIQIEKATKEGLLVSIEHDDLNG
                     LVFVPNNYVDVNEETGEYTIWINDKFDYTLTDKQINEQTSFTVKGEGIIRGLEKMQGI
                     QPQEIKLSSKNILSMSNRGVSISIPELSIKRLFIPSEYVVIDEISKSCSILIGEKWNY
                     YAEPLPENDDNSNKKLPYQKFTGRSLISSLKETPELLDVFLKPKFDQLHYQSRKRYTN
                     QLVEALNTIREEKIVSASQLEQRLNEIVEQKNQAKEKIAEIDGKIKNYNNVAKLLVTY
                     EKYLPIMNEIERATMLQKIKLEQKYKEEVCQFKFAEKKLLASDNLRPDLTKEKIISVA
                     KNQEKQRKRLIEGYKHYENKLNRLIDVQEILQELDRNGLFDLSGYRLINREEKLTDKI
                     EKYEKEVEETQQKNSKDKSKEREI"
     gene            3667..3879
                     /locus_tag="IW728_002357"
     CDS             3667..3879
                     /locus_tag="IW728_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025695.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAIAKASDSVLAWLKFKKEQKK"
BASE COUNT         1716 a          584 c          756 g         1297 t
ORIGIN      
        1 attaactgta gaaagaatta atgaaatacc tgattgtctt gaaaaggatc atttatttct
       61 aattagaaaa atgctaaaag atgagaatct tgaacccaat gattttattg ttgttgaata
      121 tccagctact ggaatatatt gctgtaatca tattaacaac ggtcaatatt ttattattca
      181 agaaggaaaa aataaattag ttccatacta tactacttgt gatatgaatg aagagggaat
      241 taataatttt ccttgtacga gtatcaaaga atccatgaaa ttaatagaaa tgtaaaagag
      301 agggacatat aaatcatgca aacatttact caacacgaat tagaaattat tagcggacac
      361 aaacagattg caggttcaaa tgcaaataaa ttaagcgtca taaaaagcga agttggagaa
      421 atgctttcaa tgttagaaag tgatttagga aaacaatata ttgaagatga tgaacaacga
      481 cattacacgt taaagaatta tcagtctgtt tatgataaac tcgaaaaaat gagtgaagat
      541 gaatttaaac aaattgattt aagcggtatt gaagaattgg aatagaaacg gggattgggg
      601 cagcgcccca aaatgcaaaa agaagaagtc gcgtgagcgg ctttttcttt tttttccgca
      661 agttggtacc aacttgcaaa tcttgctgta agaccgcccc cctgaaatct tcgatttcta
      721 atcggagggc ggtcacacag tggaaaattt cgtttttaaa caaagataaa acactaaact
      781 agtgaaaaaa aacaaaaaat attggtaagg agggggaaat atggagcaaa aactagctaa
      841 aactcctgaa aatattctac aaatcgctaa aacttacgac aacgatgaaa gaaatattca
      901 aaaaaaattt agagtatctg aaagagaaaa attcttaata gactacaaaa tgagtcaagc
      961 aaatacaaga aatttttccc gatacgctcg tattatgtgt ttaagtggtt taatgattac
     1021 aacagatttt tctgatttaa aaaatttaac tacagaatta ggacgaattg gaaataatat
     1081 caatcaatta gctcacagag ctaatatttc aaacagtgtt tcaaaagaag actttttaga
     1141 attaaaaata aattatgatc aattgactaa attaattaac gaagaattac tttttagaat
     1201 gcgacgtgct agagataatc agaggtttat ttaatggcag tgacaaaaat acaccaaata
     1261 aaaactactt taaataaagc aattggctat attacaaatc cagaaaaaac aaataatgga
     1321 atgctggtta gtagctataa ttgtcaaagt caatttgcag aagttgaaat gcaaatgact
     1381 agggaatatg cacgtgaagt aaaaggagat tatcgaaaag tcggtgggag tgaggtttta
     1441 gcgcaccact taatccaaag tttttcgcct gaagacaaag tgacaccaga gcttgctcat
     1501 gaattaggac gacaattgat tgatgaattg actgaaggaa agtttgaata tgtgattgcc
     1561 actcacatag atcaagaaca tattcataat cacattattt ttaacagtgt ttctttttat
     1621 gatctgaaaa aattcagaag tcaaccttat cgaacagcaa ataaaattaa agaaattagt
     1681 aatcgtattt gtgctgaaca tgatttatct ttatcaccga aaaaagaaaa attaaaagat
     1741 tcctacaaat cttattctaa cagaagaaat aacacgagct ttcgttctga aattcgtaaa
     1801 cgattgacca tgtgcttaaa tgaatcaact tcatgggagg aatttgaaga aaaagcagat
     1861 catttagggg taaatattga tcgaaaaggg aaacatattt cgtatgcttt attatccgaa
     1921 ggacaacaaa gacggactag aggggacaag ttagatgact tagaaacatt tacagaaagt
     1981 aatatttccc aaaaactttc tgctaatcaa tcctacatta atcagttgaa agaagcgatt
     2041 gaagagacat tcttttctag tgacaacttg gcagatttta gttttctttt ggaacaagag
     2101 catggaatta cctttaagtt aaataaacaa gatcaatttg ttttccattt caatgatgta
     2161 aatgatttta aatggaatga aagcttgctg ccagatagtt atcagattaa aaatctaaaa
     2221 gattatttta gagaatccta tgagtttacg aatgactctt ccttaaattc aattgaagaa
     2281 agatatcgtg aaaaagaaaa aagtgtgatt gttcaagaag atacgcctat tcaattacaa
     2341 gaaattcaga tagagaaagc gaccaaagaa ggattgttag tgtctatcga acatgacgac
     2401 ttaaatggtt tagtgtttgt cccaaataat tatgttgatg taaatgaaga aactggagaa
     2461 tatacaattt ggatcaatga caaatttgat tatacactta ctgataaaca gataaacgaa
     2521 caaactagtt ttactgtaaa aggggaagga atcattcgtg gactggaaaa aatgcaaggg
     2581 attcagccac aagagataaa actttctagc aaaaatattt tatctatgag taatcgtggt
     2641 gtatctattt cgataccaga actgagtatc aagcggctct ttattcctag tgagtatgtt
     2701 gtaatcgatg aaattagtaa atcttgttct atcttaattg gggaaaagtg gaattactat
     2761 gcagaaccac tccctgagaa cgatgataat tcaaataaaa aattacctta tcaaaaattt
     2821 actggacgtt cattaatcag tagtttaaaa gaaactcctg aattacttga tgtgtttcta
     2881 aaacctaaat ttgatcagct acattatcaa tcaaggaaac gatatactaa tcaattagtc
     2941 gaagctttaa atacgattcg tgaagaaaaa atagtttcag cttcacaatt agaacagaga
     3001 ttaaatgaaa ttgttgagca aaaaaaccaa gcaaaagaga aaatagctga gattgatgga
     3061 aaaataaaaa attataacaa tgtcgcaaaa cttttagtaa cttacgaaaa atatttaccg
     3121 atcatgaatg aaattgagcg tgctacgatg cttcaaaaaa ttaaattaga acaaaagtac
     3181 aaagaagaag tatgccaatt taaatttgct gaaaagaaat tactagcttc agataatcta
     3241 cgtcctgatt taacgaaaga aaagataata tctgtggcga aaaatcaaga gaaacagagg
     3301 aaacgactta tagagggcta taagcattat gaaaataaat taaatagatt gatcgatgtt
     3361 caagaaatat tgcaagaact agatagaaat ggattatttg atcttagtgg ttatcgtttg
     3421 attaatagag aagaaaagct aacggacaag atcgagaagt atgaaaaaga agtagaagaa
     3481 actcaacaaa aaaattctaa agataagagt aaggaacgtg aaatataaat aattgaaaac
     3541 actatatatt ccgaaaagtg aatatatctc ttcataaaat aatcaacata tttaaaaaat
     3601 tcaatcagcc ttttttgtcg agataaagtt cttcattcta tgatataatg ttgctgtaga
     3661 aagtttgtgg cggtttggca aatccaattg aaggagcgtg gtgcctatgg gcttattagc
     3721 ttatcggacg tactctgtag aaaggaaatg cctgatttgt tctcattgga tcaaatcatt
     3781 gctattatcg gagcagcagc agtagtaatt actgctatag cgaaagctag tgattcggtt
     3841 ttggcatggt taaagttcaa aaaagaacaa aaaaaataga gccgtctcaa cttttggcga
     3901 agtagacggg ctctaaacct gatcaaaaat gccatccgct ttgaacggac tacactgggc
     3961 atgttacagc atgccctttc atttttatta taactcatta atctaaattt gaaaagtaac
     4021 tttgtttgct aaagggtgtt ctacatgaca ataaacatag catatatatt ttatttttat
     4081 attatcaact ttgaaaaaat ttattctctt ctagaaatag tatgtgaata tattacaatt
     4141 gaaggattaa ttgctacttc ttcgatatta gtaggattat taatcccaat tgctattcta
     4201 ttacttgaaa atagtagtaa tctcattgat cagtctttca aatgggataa acttgtaatt
     4261 ttttcgcaag ttattaaaat tcgttccatt attttgggat taacatttat aacttttcct
     4321 ttaattttaa atgataaaac taaaaatatt ttc
//
LOCUS       DACEMO010000183         4273 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEMO010000183 DACEMO010000000
VERSION     DACEMO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.189"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..162)
                     /locus_tag="IW728_002358"
     CDS             complement(<1..162)
                     /locus_tag="IW728_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1025696.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFI"
     gene            complement(471..1184)
                     /locus_tag="IW728_002359"
     CDS             complement(471..1184)
                     /locus_tag="IW728_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1025697.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(1438..2139)
                     /gene="nagB"
                     /locus_tag="IW728_002360"
     CDS             complement(1438..2139)
                     /gene="nagB"
                     /locus_tag="IW728_002360"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1025698.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            2373..3905
                     /locus_tag="IW728_002361"
     CDS             2373..3905
                     /locus_tag="IW728_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1025699.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(4096..>4273)
                     /locus_tag="IW728_002362"
     CDS             complement(4096..>4273)
                     /locus_tag="IW728_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1025700.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         1383 a          876 c          732 g         1282 t
ORIGIN      
        1 gataaaacca gcaacaagca atggtaaaaa aattgccgaa agcattttca atattggact
       61 aaaaatattc ggcatttgta aaataaagta cacgccgatg atcgtgagta ctagctccac
      121 tgtccagaaa aataattttg attgtttaaa acgtgattgc atacatctcc tccaaggtcc
      181 tttgatactt ctattctatc atttaacaaa gagttacgcg ctgagatttt catttgctta
      241 aaaaaaacaa aaaaaataaa catattttaa tgagtagacg agtacttaaa caaaaacacg
      301 gtttggagta gtgacaaaaa ttatactgca gtcccttttt ccaaataaac ggtgttcaaa
      361 agcttgcatc ttcgtaataa gcccagccaa acgaaaaata taaggaacta tttttatttg
      421 gctgggctta ttacttgatg taggacatct attttacaac ctcagatcat ttattgcgca
      481 atactgacta aatgcaactt tgtgatattt ttgtgtacgg acaactcctc gatcacatcg
      541 gcataagtga gcccttttgg aagatcaatc gtatagatat ttttgtacac tcggtagtca
      601 tcaacgaatt ttacgtcaaa attcacatct tcgattacga tatttttctc ttcaaaataa
      661 tcggtaagaa aatctttggt ttcttttcta tggataaatt ggatttctaa ctttttagtc
      721 gtaggtacat ggatcacgcg tttcactact gtgagtgcaa tagcaatccc gataaaactg
      781 atgatcgcaa tggaatagta acccatgccg atggcgatcc ctaatccagc aattgcccat
      841 aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg tgacgatgat cgttcccgcg
      901 cctaagaaac cgacaccact caccacttga gcaatcagcc ttgcttcatc tgaacgaatc
      961 gttccagcaa gctgtggata ctctaaagca tctcttaatg caccactagc gatctcaact
     1021 tggatcaatg caatgataca tgctcccata caaactaaaa tatgcgtacg catacctgct
     1081 ggacgatttt tatattgtcg ttcaaaaccg attgctccgc caaatagcat agcgatccct
     1141 aaacgtaaaa taatctctgg aattgtcaat accatatcat tcatctaaac accctccagt
     1201 ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc aatttttacc tcttacgaaa
     1261 aaaggactgt gacaaaattt ttgtcatagt cctttttatg aataaacggt gttcaatcag
     1321 ctgaccctcg gtattactca acaaacggca aaatatgggg agctattttt cctttgtctt
     1381 ctctaatact caggtcaaaa cgctgatttc acaacctctt tagcctacta ttgaaaatta
     1441 caacttactt gctgcttctt catcgatgat cacaacaaca ttgtcgtgat tttgcaatac
     1501 acttgctggc aaatgattcg ttacaggtcc ttcaatcatc cctttgattg catcagcttt
     1561 gctttcgcca aatgccatca agacgatttc tttacttttc ataattgaac cgattcccat
     1621 tgaaacagct tttgttggta catcttctgc tttatcgaag aatcgtttgt ttgcattgat
     1681 tgttgattct gttaagtcaa ctactgaagt cgttccgtca aatgaagcac caggttcatt
     1741 gaaaccgata tgtccatttt gcccgattcc taagatttgg atatcgattg gatgtgcatc
     1801 gatgatttga tcatactttt tgcatgctgc ttccagatct tctgcttttc catctggtac
     1861 aaacgtttct ttgaatggtt ttttatcaaa taattggaca tccatgaaat gacggtagct
     1921 ttgttcatct gaaccgccta gtccaacgta ctcatctaag tttacagaag tcatttcact
     1981 gaaatccaaa tcgcttgaag tcatttcttt atataaagta actggtgtgc ttcctgttgc
     2041 caatcctaat acttttgcac ctttgttcat accttctttg atgatttcaa atgctttttt
     2101 tccgccttct tcagcgtttt ttacacgaat gatttccata gtgattgctc ctttatattt
     2161 tggtatagcc caattatagc attctttttt tctttttaca ataattagta tagaccaaat
     2221 tgcatttttt tcattttctt gacaataaat aggaatccgt gttaggtttc ataagatggt
     2281 tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg attcactgtg gacgctttat
     2341 gtctgcagtt tttttatgaa aaaggagtta ttatgagtat actgacatta aacaacatta
     2401 cccagcaatt tggcgacaaa atcctctatg aaggtgtcga tctgcaatta aatgctggcg
     2461 aacatcttgg gctgattgga caaaacggag ccggtaaaag tacattgatt aaaatcatca
     2521 ctggtgagat cttaccagat gacggacaga tccattggca aaaaaatatt cacaaaggtt
     2581 acctcgatca atatgtggaa gtcgatgaaa cattaacaat cgaagaattt ctaaaaactg
     2641 cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt tctttaccaa acatatagcg
     2701 agaccttcga tgattccttg ctggaaaaag ctgggaaact ccaaacggaa ttagaccaag
     2761 gagtgtttta tcaaatcgac accttagttg ctgagatgag cagcgggttg gggatcgatg
     2821 tcttaggttt agattcactg ttgaaaaatc taagtggtgg acaacgttca aaggtgattt
     2881 tagcaaaact acttttagaa aacccagatg ttctgatctt agatgaacca accaatcact
     2941 tagatgatca gcatatccaa tggctgacgc agtttcttca ggattttgac ggtgcgtata
     3001 tcgtgatttc ccatgaccaa gaatttcttg atcagatcac cacccatatc gcagacatcg
     3061 agtttggaaa gattactaag tatactggcc atctaaaaca agcattgaaa caaaaggaac
     3121 aaaatcgcga aagctatttg cgccaatatc atgctcaaca aaaacaaatc gaaaagaccg
     3181 aagcttatat tcgaaaatac aaagccggtt cccgatcaac gatggcaaaa agccgacaaa
     3241 aacaattaga taaaatcgaa cggctgacgc ctccagcttc agcaagtaaa gctgtctttg
     3301 attttccatt ctcgcctatt gtcacgacat tagcagtgga aacaactgat ttagtcatcg
     3361 gatatgatcg accgctactt gcacccatca atttgaccat ccgctttggt gaaaatattg
     3421 ccattcgagg cttcaacggg attggaaaat ctactttatt aaaaacactg atcggtgaga
     3481 ttgaaaaaat aagtggcgat ttccacttcc cagataacac aaaaatcaat tacttttcac
     3541 aagatctgta ctgggaaaat ccattagaaa caccactaca atatttaagc agtcaatttc
     3601 caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa agcagggtta gttaatcaat
     3661 tagcaagtga acctctcact acattaagtg gtggggaaca aactaaggtg aaacttgcac
     3721 agttgacaat gaaccaaggt aatttactga ttctcgatga accgacgaac catatcgatc
     3781 aagaaacgaa agaaagctta caagacggga tacagaaatt tccaggaaca gtaatcatcg
     3841 tctctcatga acaagaattt tatcaagatc ttgtgcaacg agtcattgag attgaaccaa
     3901 aataaacgtt tgattgaata gttgaaaaaa aggagctgtg acaaaagttt tgtcacaact
     3961 ccttcttctg aataaatggt ggccaattag ctgaccttcg gtattagctc aaaaaacggc
     4021 aaaatatgag gagctatttt tatttggcta ttcttattac ttgatgcaga acagcttttc
     4081 acaacctctt ttcgtctatt ttttattttc gagatttctc tttcctttaa aaacaactgt
     4141 ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta accgttacac ctaaccagcc
     4201 atactgctgc cacattgtcg tccctagcaa tgaaccagcc gcacctccaa taaagtacat
     4261 aaacataaat aca
//
LOCUS       DACEMO010000184         4272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEMO010000184 DACEMO010000000
VERSION     DACEMO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.190"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..357)
                     /locus_tag="IW728_002363"
     CDS             complement(<1..357)
                     /locus_tag="IW728_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025701.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNL"
     gene            complement(361..669)
                     /locus_tag="IW728_002364"
     CDS             complement(361..669)
                     /locus_tag="IW728_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025702.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(678..1097)
                     /locus_tag="IW728_002365"
     CDS             complement(678..1097)
                     /locus_tag="IW728_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025703.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(1090..1323)
                     /locus_tag="IW728_002366"
     CDS             complement(1090..1323)
                     /locus_tag="IW728_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025704.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(1326..2294)
                     /locus_tag="IW728_002367"
     CDS             complement(1326..2294)
                     /locus_tag="IW728_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1025705.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(2741..4147)
                     /locus_tag="IW728_002368"
     CDS             complement(2741..4147)
                     /locus_tag="IW728_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1025706.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
BASE COUNT         1204 a          968 c          601 g         1499 t
ORIGIN      
        1 taaattcacg tcattaaata ttgacgtaaa tttactctca ctcatctgac ttttcttatt
       61 cggtatcact ccgaataagc cgtcagattc atcgggataa tctcgttcgc ttggcgctga
      121 tttttcatca gcaagccact cattatccac tgatttttcg tgttgctgat cgtcagaaat
      181 cgacgatagc caccgctcaa aatcagcaaa agtttcaaac ccaaaacgct tgcgaagagc
      241 cttgcccatt gcagcatttt ctttctcaat caagcgtttt ttctgttggc gaagtttcgc
      301 ttgttctttt tttaattttt cttcttgcgc tttaatatct gctaaagtca ttcccataaa
      361 ctacatctcc ttttctttgg aattaataac ggaaatttgg tttaactgca taacatgact
      421 cattaatttc aaccaaaatt ctttttctga ttcatcagtt gtttgcgcta aatgcttatg
      481 aacaaattca ttagtcaaaa ctgaagattt tgctagatca gcattgataa ccgagttgag
      541 aacatccatc attcgttcaa gcatttcact ataattatct tcatccggca aattcaaata
      601 atcagctaac cgttcataca tcaattcaat tctatcgact gtttcttctc tcaaatcaat
      661 tttatccatg tcacacctca cttccagaat tgccaccatt ttcgattttt taattcttga
      721 ttttctagta acaactgatc gtttttttgt tgctgaagca aagtcaattt ttgttggtca
      781 cgaatcattt ccgttaattc agaaatatgc ctatcttttt ccgctaattg ttcacgcaaa
      841 aattcatttt ctttttctaa cgggaaatca ttgcctgcat cattgaccgg taaatcacca
      901 gtcaattgac caccttttga ccgataaatt gaccgcacaa caccaatcaa ttccttgtct
      961 aacatgataa ccccattatc tttataccga gcaaggtcat aacctgcatt gaccagttga
     1021 ttgcgcactg tatttttaga tatacccaac tcatcagcaa ggttttttat ggtcattttt
     1081 tctggcaaat cagtcattat cttctccgtt actttcagct accccataaa catatttaac
     1141 agcatgctta gggataatga ttggggaatc aattttatta tttttcaaaa tcaactcata
     1201 ctgcgttaca tcaagtaact taccggacat ttttttgcta tccaaaaaaa caacgacaac
     1261 gttatcacct gttaaattca ccaaccaatt tttcttacca aaatgctttt gtttttctgc
     1321 cattcttatt cctcctccgt tacatctaac ccttgaagcc aagtgtctaa ttcttttttc
     1381 aaaacatctg ctgattccga aaatgtttct cgtaaatgat tcgtcaactc atcattttga
     1441 ctttctcttt tcaaaatatc tctcaatttg tatttcattc tacgctctaa aaacccccac
     1501 atttcagggt tagcaatcag tccaaaaatc atcaccttat catttgtttt tacatcaagt
     1561 ggtagaaaat gagggctagc aaaactattt aaactttcat gaaccattgc atgccaatct
     1621 gttgctttcc cacgccttaa ctgcaattcc agtcgccacc aagtttctat ttctggtggc
     1681 acaattttac gcttggtttc ctgttccaat tttttgttgt atagccgaat ctggcgttcg
     1741 ctggcacgac tgccccaata agctgtttcc agcttcccag accgcccata aattggcttg
     1801 aaagaaacag gatcaaccac tcgatattgt gtaataaact catcaggcac atcgataata
     1861 tcgcaagcaa tatccgcacg tgagaaatgt ggttctagaa ataaatcatg aatgaaattt
     1921 ttcattgaac tagctaaaaa atgaccaatc ttattcgggt taaaatcaat ccgccctttg
     1981 ccatcttgcc atttcaaaat ctcaatataa gcaatatttt cttcgtattg gtctaataat
     2041 tgccagccgt tattcccaac cgcttttaaa tagccattgc cttcattcac ccgcattaat
     2101 tgctcaaaat ctaaaatcaa cacatcattt tgagtgtgat aataaatatt ttccttcagt
     2161 tttccaacga tagtaattcg gtcaattgac cactgcattt ttaagggggt gtacatattt
     2221 tgtacattta accgcctgtt agatagggct gttaaatttt cccactcatt tacgattatg
     2281 ccatttttac tcaaattttg ttcctcctca aagaaaaaga gacttctgga aagaattttc
     2341 aaaagttcac gtaactctta aaaattcatt accaaaaaat ctctagtctt gaagaaatct
     2401 aaaaatattc gtataatgat taagaagtta cggagctagt gcgattagtt tccgtaaagg
     2461 tcgagaacgt tggtagcgtt gctcgactat ttttttactc ttttttcagt tcatttgttt
     2521 gctaactcaa taatagcact tctctttagc tatgcaaata tccaaaaaat aaaaccgcac
     2581 cctttgaaaa gggcgccccc aaacttcgct aacgctaggt tggccaaaaa atggactggc
     2641 cattttttag caaatgtcag cttaataggc tagtggttta gccgtcaact ccttatcctc
     2701 ggcacaaacc gcctccggta ccgttgactg cacatcgtaa ctaccactcg atctcatctt
     2761 cagaacgctc taatttacgc tgtgagacgt tttgacgata aaataaggtt ttctcctgct
     2821 ctaacacttc taattcctca gaaagcccca tatccttttc tgcaagacca aaacggatag
     2881 acaaacgagt gatcgatttt tgaaatgcgt tttttaatcg ctctaaacgc tggcgtaaag
     2941 agcgattttc ttctttcagc tttttatttt ccacttgtaa atctgctaat tgttttctac
     3001 cagacaaatg acgctcttct tccaatgtag ctacatactc atttaattct gtattttgct
     3061 caagcatctg taaatattgc tcactcatgt tatcagcatt tctcttggca tttgttgctc
     3121 tcgtagctcg ttgatacaaa tcaacaaaat cattcccaga taaatgatac gaaccatcag
     3181 gaagcctctt gaaactttct aaaggaacca tatctttttg gaaaatgacc gccatctctt
     3241 tttttacatt cagcatattt ttcttctctt cttcaatctt cgctctctca gaagccatca
     3301 gagccttatc acgctcgttt tcttctcttt gcctagaaat atacctcaac tcatcagaaa
     3361 cgctctcacg ctcttttaca gccgtttggc gcaattcaga aatttcactc atgatttctt
     3421 tgtattcgtg aatatcttta attttgttgg taccgacaat atcccttttc caaccgtaat
     3481 cattcattaa tcgttctata gagtcaactt cttgatttct aaaattattg aaaagcactc
     3541 gaccattatc tttatcagcg tgtatccctt gttgaaccag tgctttatta aagctcggct
     3601 gcttttgaac accccgttta taaccttcag caaccggcac aatattcaag tgcaggtgcg
     3661 gacttatttc gtcattatga ataacagcat tataaacagc caaattaggg tttctctctt
     3721 gaaactcctt gtaatactga accaaaattt tattggcaat cactcgattt tgttcagctt
     3781 gttgtttcga tatgccagta gaagaaccat ctgctcgatt tgtctggaaa tcatctaaaa
     3841 tgcctatttg cacaataaat tctcgttgag gttccaactt tttatccttg cgcactttcg
     3901 ctaaatagtc ctcgattttt ctatcggctc ttttttgttt cgcattatat ttttctacca
     3961 cttcgccaaa caattcctca tatttatctg ctagattcgt ctgctctaaa taaatatttt
     4021 catccgattt cgtgaagtca atatgcttat gccaatcatt ggttttctgg cgatcagtta
     4081 actcacgatt attatgagaa atacttgtat tcttcgttcc ttttttaaat gaaatactaa
     4141 accccaagtt aaaaccctcc ttcgcgctgt cgctaataaa aagattgtaa acctttctgt
     4201 tactctttgt caaagagtaa cattttccta attgtgacat ggctgtcaca attccaaatg
     4261 ctctccgagg gt
//
LOCUS       DACEMO010000185         4266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEMO010000185 DACEMO010000000
VERSION     DACEMO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.191"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            184..534
                     /locus_tag="IW728_002369"
     CDS             184..534
                     /locus_tag="IW728_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1025707.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            515..1231
                     /locus_tag="IW728_002370"
     CDS             515..1231
                     /locus_tag="IW728_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1025708.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            1231..2679
                     /locus_tag="IW728_002371"
     CDS             1231..2679
                     /locus_tag="IW728_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1025709.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2749..3258)
                     /locus_tag="IW728_002372"
     CDS             complement(2749..3258)
                     /locus_tag="IW728_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025710.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(3248..3973)
                     /locus_tag="IW728_002373"
     CDS             complement(3248..3973)
                     /locus_tag="IW728_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025711.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1502 a          712 c          867 g         1185 t
ORIGIN      
        1 catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat
       61 tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct
      121 gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa
      181 agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt
      241 ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca
      301 gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta
      361 ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt
      421 gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta
      481 cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa
      541 tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt
      601 caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat
      661 gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg
      721 caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca
      781 ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac
      841 gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga
      901 agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa
      961 aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga
     1021 tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga
     1081 attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa
     1141 attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg
     1201 ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct
     1261 ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca
     1321 aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa
     1381 aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa
     1441 aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc
     1501 gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca
     1561 gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat
     1621 attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa
     1681 ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca
     1741 attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc
     1801 tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa
     1861 gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag
     1921 ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa
     1981 gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct
     2041 cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag
     2101 cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat
     2161 gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc
     2221 aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca
     2281 ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga
     2341 tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa
     2401 atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga
     2461 attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct
     2521 tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt
     2581 gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc
     2641 gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat
     2701 catgaatcga tcttctatac cgcttgtttt tactataaaa tgattgattt agcccgcggg
     2761 ctctaaacga tcaatataag gactttgagc aatagtagca tcatgagtaa agagtaagat
     2821 accttgattt tcttcatgag ctaaataaga aagcaagtgg agtagctctt ggatctccaa
     2881 tacactaagt tcatcaaatg tatcttcaat aacaatattg tttcgcttgg aaagcaaggc
     2941 atgaagtaat tgcagcttta ctttttcaaa agacgtcaac tcagctgccg cacaattaag
     3001 caaggaagga gagatatcaa gtttttcttg ataggccaaa cgatcttgtt tgatttcttt
     3061 ttccggtaca tccaacagga catggtcttt aagattcaga taaggaaaca tcggccaatt
     3121 tttttggatg atcgcaaaat tatccagttt tgcaacaaga acctcttttt tcaagtgctc
     3181 caatatttta tgctgttttt cgagaggaca caaaataacg gtcaagtgtt cagcatgaat
     3241 caaatattca ggtttcatta cgtccaggcc tcctactttg caaatgatgg ataaaaaaag
     3301 tgaaagggaa tagactaaga atgagacagc atccactgag cccaataaga gtttgggcaa
     3361 atcccttaag tatatctgtg aaaaaaagat cattttggaa atcgatggta aaaaaagtct
     3421 gttgatccat cggaattgta agtccgctta ccgaagtcaa gatatcacgg atatctggca
     3481 gttcagtagt ctttaagaca agaaaattga tcctttgaag aagcgcttca taaactgact
     3541 ggaagcttac gatccacaat aaaaccaaga tacaacaaag aaagagagga aagaacagct
     3601 gccagaaaat aaaaaggaat aatttggttt tagaaagacc agtcaggtgc caattgatca
     3661 attcctgctt gttttgcctg actgaatagt aaaagaaaaa gataaaaagg aaggcaaata
     3721 gaaaaaataa actgaatatg ccttttatat aaaatcggct agctaattct aaagccggaa
     3781 gaagctctgt agatgcagaa gcgccaataa atgaaagccg atcttggact tgattggcta
     3841 aagccgattg caaatggtaa agagtcaggc atgctatcaa taaaaaagca gaaaagcttg
     3901 aaaataaaca atagagtatt gtctgctttt tgtaataaat taatcgagca gtagcatagc
     3961 ggattatttt catacttttc acctctataa aaagtataga aaatgaatat gaataaagta
     4021 tgaaattcct ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa aaagcaagag
     4081 gttgtgaaga agctgcccta ttctattacc gtagatgcaa gcttttgaac actgtttatt
     4141 cgaaaagagg agcagcgaca ataattttgg gctcttcgtc aatgagtgtt ggtgaagaaa
     4201 ttcaatagaa taatcgagat actcacacgg ctagacagct atcttgtcta gccgtgttgt
     4261 tgttct
//
LOCUS       DACEMO010000186         4242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEMO010000186 DACEMO010000000
VERSION     DACEMO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.192"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(103..915)
                     /locus_tag="IW728_002374"
     CDS             complement(103..915)
                     /locus_tag="IW728_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1025712.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1078..1185
                     /locus_tag="IW728_002375"
     CDS             1078..1185
                     /locus_tag="IW728_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025713.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1243..2310
                     /locus_tag="IW728_002376"
     CDS             1243..2310
                     /locus_tag="IW728_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1025714.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            2452..2655
                     /locus_tag="IW728_002377"
     CDS             2452..2655
                     /locus_tag="IW728_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1025715.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            2715..3917
                     /locus_tag="IW728_002378"
     CDS             2715..3917
                     /locus_tag="IW728_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1025716.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1412 a          677 c          879 g         1274 t
ORIGIN      
        1 ttctaacagg gggctaacaa agaacaggaa actgtgacaa ctacccattg gggagtgtcc
       61 tagcagtatt ttctttgtca cgtgcttatc aattttgtca cctcattttg atgatggata
      121 cgctgcattt aacaggcgtt ctttctcgac ttccatcacg aatggtagat aaagatattg
      181 ctgttctagt agatattgat tgtcctctgt gtactgataa gaagcggttt cttctgaata
      241 gaccaacggg gcaattcgca catcgctggt caattgatta attgagtata gaaggatggc
      301 tttctcacct agttccttgg gtaaatgttt cccaaaatag gaataggatt tttgatttgc
      361 taaatagacg agtatgcgat ccacactaaa gttggagaag gtaaagggca tcatgtgcag
      421 aatatgattt tgattatcat gttgcaaata gagctgggct tccttaccaa aattagttga
      481 accaagataa agctgctttt tttcatcgat acattggaaa gtaaactggt cttgttcagt
      541 tggtggaatg agatttgtaa taaaaaattc aatcattcga tccatttctt tttcactaac
      601 aaggtcagcg tgtttgattt cgtctactat gagaccttca atcttaggat caagcttacc
      661 tgcctttttt tggagcattt ctaaaatgta ttgattctct tgatcaccat acaaaagata
      721 ttcattggtg acatgaccta tctctgcgat ttggtgaagg gtttcggttt ttggaaggtt
      781 gatcccacgt tcccagttat tgactgtact tcgcggtaaa tcaccaatta gtttgccaaa
      841 tttctccata ctcaacttta attcttggcg aatctgccga attcgattgc cgactgcttg
      901 cttgtctatc tccatgaaaa ctcctcctca ggataagttc ttaaatttag tatacgtgtg
      961 tacttaaaaa aagtcaaaac atattgactt aaaataaata caggcgtaca ataaaaatgt
     1021 acttaaaata agttcaatag gttctttgac aagtaaatag cagaaggagg tttgtcgatg
     1081 aaccaagtta atcaacatga tctcggtgaa gccattcgag tttctcgaga agaaaggggg
     1141 tggacacaac gatatttagc agaaaaagtg gggatttcga gatagttatt atcgaaagta
     1201 gaaaaaggaa cgagacaatt aagtgaagaa aaattgaatc ttgtgttgga tagtttgcaa
     1261 gaagaggtcg ttccagttaa ccgtgttttg attgattatc taaccattca ctttttctcg
     1321 aatcaacatt taaagctaat tgaagagatt attgaaatgc ccattgaacg ttttgaggaa
     1381 ctggattatg cgccaaaggg ctatattggc caatacgtgt ggaatcaagt aatcacgatt
     1441 cggtattcca ttgacgatgc ggttaaagga actgtgatgg agttttcagg tcaaggctgt
     1501 aaacacttag caagatggct aaaaacacga aaaacaaatt ggcaacagtt ttttcgtaag
     1561 gtattagatt atcaggggaa ctttacacgg attgatttta ctttagatga ttttgtgggg
     1621 agtctcagca ttccagaact taagcataag gtaatactag gccatgtgtg gaccactttt
     1681 caagtgagtg aatctcatgg cggtacggat atcataaaca atgaatcaaa tggtgagacc
     1741 ttgtatttag gctcaaagaa gagtcagtgt cgtttttgct tttatcagaa ggaccatgaa
     1801 caacggaagc gacgaggaat tcctttagaa gaggcaaaag taaagaatcg ctttgaacta
     1861 cggtatcgaa aagagaaagc gcaaagcttg gcaaagatta tttcaaaaac ccatgattta
     1921 accaaactgt tctttgagtt acttaatgga gcaatttgtt tttatgaccg tgatccaaat
     1981 gatctaggtg caaaagttga tgccaaatgg gcagccttta ttagcaatta tggcgcaatc
     2041 accatttctt tagatacaat tcctcaaagt tttgagaaaa ctatgagttg gttaattcat
     2101 ggtgtttcac catcattagt ttttattgca caggtcgatc aaacgtttca ttcttccttg
     2161 ttgcctttga ttattgaaca aggagaaatg aatccaagac agatcaaact tctccaaaat
     2221 atgaaggcag acccggagta ttatcaggag gaggttgagt attatgaacg aaaattacaa
     2281 agtatatcaa atgagaaaac aagctactaa aaaagcaaag tactgcttac acaacaagtt
     2341 gtgcaacgta ctccgcctcg acaagacgag tataacacaa ccatcgaatg agaaacaata
     2401 gattataaag aggattattt taaaaggagt aaagaatcag gaggtgagag aatggaaaca
     2461 aaatgtgatt tcatggtaaa tcgtgcaatt ttgatagaga tgggctttaa acctagtcaa
     2521 gctgcacgaa tgattaaaga gagcaaggca tatcttgcaa ggattgaagg tattgatttt
     2581 tataataatc gccaagttgg tgtggtgcca tcccgagtaa ttgaacatct ttttcacatt
     2641 caagttgcgg aataaacagt acaagcgcgg tactattaaa gtagcttttc tcaagtacag
     2701 ggaggaatgg aacgatgcca aaaattaaaa atatttacca agataaaaag acaaaaaaat
     2761 ggttcttccg agcgtatcta ggtatagatg aaaatggaag aaaggttcag aagactaaac
     2821 gaggttatgc ttcacaaaaa gatgccaaaa ttgcttatga caagtacatg gaaacacatg
     2881 attttaacaa gacaattcct aatcaactgt gttctaccaa tcaaatgact ttcgaagagt
     2941 tttacaagga taggtttgta aaatggtacg agagacaggt caaaagtcaa acatatgaga
     3001 atgcgcaatt cattttcgaa aagcgtatgc agtttttcta tcattttcgt gttcgagata
     3061 ttacaagtca cgatattgag gattggttat ttgaactaag ccagacagag tcacgtaatt
     3121 cacggaaaaa agttaatggc gaaacgttat caaaatctta tatcaatcgg attcgagggc
     3181 acctaaaaat tgtattggat cgggctgtta aagagggggt gattgctaaa aatcccgttg
     3241 aagatgtttc ttcgcttcct tctgagaata agaaagttga gttttgggaa cacagtgagt
     3301 ttcaaaaagt gatgaatgaa ttgaaggatg ataagattca aacccaccat cggaagattg
     3361 tgtatgaaat gctcttttat actggcattc ggattggaga acttgaagct ttgtcttggt
     3421 cgaatgtaga ttttgagaaa aacacgataa cgattgagaa gacattgatt tataatacaa
     3481 aagatgattg gtacttttcg actcccaaaa ctaaatgtgc ttatcgaacg attgggttgg
     3541 ggaagaagct ttccttaaaa ctcagacagt ggtatgactt gcaaaaacgt attggaaatt
     3601 ttgaatatgt tgctcagttg gacggaacat ttacgccacc ttattctttt gcgaactggt
     3661 taaaagaagc tgctaaaaaa gcgggtgtca aaccgattaa gcttcacgcg ctacgacatt
     3721 ctcatgttgc atttctaatt gagcagaata ttcaaccgct atctattcaa gaacgattgg
     3781 gccatgcgaa tattcaaatc actttaggaa cttacggtca cttatatgca aagtcagatc
     3841 aacaagtggt aagtgcgatt gataatgttc aagagggcgc tattctttca aaaaatgaca
     3901 atttattacc agtctgagtt ttccacggtt ctaccataag acaaataaat gcttttgcac
     3961 cagtgttttc agtcgaattg ctcctgttca agatgtatcg gaaatagcga gaaaataagc
     4021 aaatttgcat aactataata aaaaggactt agttttgatg ggaaattttt caaaattaag
     4081 tcctttttta tgtggttgaa acgggtatga tcattttaag gaagtgatct aagatgaaag
     4141 gacgactgaa caattatcat attagaaaag ctttattgta ttccacaaac aaattactaa
     4201 gaataacaaa ttagtttttg tttgttctta ttccctattg ac
//
LOCUS       DACEMO010000187         4224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEMO010000187 DACEMO010000000
VERSION     DACEMO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.193"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..607)
                     /locus_tag="IW728_002379"
     CDS             complement(77..607)
                     /locus_tag="IW728_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1025717.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            712..1263
                     /locus_tag="IW728_002380"
     CDS             712..1263
                     /locus_tag="IW728_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1025718.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1416..1889)
                     /locus_tag="IW728_002381"
     CDS             complement(1416..1889)
                     /locus_tag="IW728_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1025719.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2027..3499
                     /locus_tag="IW728_002382"
     CDS             2027..3499
                     /locus_tag="IW728_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1025720.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3496..4206
                     /locus_tag="IW728_002383"
     CDS             3496..4206
                     /locus_tag="IW728_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1025721.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1323 a          758 c          819 g         1324 t
ORIGIN      
        1 atgaggaact atttttccgt ttctttctct aatactcagg tcagaacgct gatttcacaa
       61 cctctctttc acaaccttat tttactcgtt tataattttc atcgaagtat tttccgctac
      121 ggatatcatc tggcattcca tcgtatggtt cttcgctaat aacatcttca ttgtctatac
      181 ctgcatcacc catataagtg atcgtagcaa attcaggaac aagtaatttt ggataagttt
      241 catatttttc acgagctgct tccatttctg cgatatgttc gttttggaaa gtaacagtga
      301 tttctggatg ttcttcaacc catttaggga aaaagatcat tccgtgaagt ttcttttcat
      361 ttggcataaa gtccaatgcc acgtctgtcc ctgttggctc tgtccaagtg attttgaata
      421 cgccgtccgt caatttaacg atatcagctt cttggtcttt tacccaacgg cctttgacca
      481 ttccgccatg gatacggtaa tcaacagtat ggtcattttt ggcataccat tcatattccc
      541 aaccattgtc atacgtatag ataaagtgag ttccaataaa atcttccaat tctttaaatt
      601 ctttcatgtt taagcatctc cttataaatt aaaacatgta ataaactaca tgttttcgat
      661 tactcattta tgatagaatg gatttaaaga tttgtcaagg agggaaaact gatggcaaga
      721 aaaaatacac ttcgatatat tttgttaggg ttattaagta agaagaagat gacaggatat
      781 gaattgaatc aatcgttcaa aaatgaaata ggagaatttt ggcaagcaaa acatagtcag
      841 atttatcctg aacttgcaaa aatggaagag caaggaatca ttcagcatca agtagagatc
      901 acaggtgaaa aattggaaaa gaaagtttat gaattaacag aagaagggaa ggaacaattg
      961 gcagagtgga ttcatacacc caccaatgaa ctaccggtta atcgagacga atttgttctg
     1021 aaattgtatt tcgtcaaaga cgtgaatgat ccggcattag tagaaattat tcagcagcag
     1081 cgtgaccttc atgaagaaaa acgacttcat ctattagagc gaaaaaaaac gatctttcct
     1141 actgaaaaag aaaaacatga aaattacgga cactacctga ttttagcgca tgcgatcaat
     1201 cgggaaacag aatatgcaaa ttggctaaca gatgtattag aagaaagtaa ccgaaaaaaa
     1261 taaaaaaata tccgaacagt gatggagaat tcctttctat atgaaagaaa cgcagatcac
     1321 cgttcggata ttcttatgtt caaacaggcc ttttttcaag tatttatgta gcatctactt
     1381 attaaaaagc ttggtactta ggagattagt ggcgtttaga gttcttttaa caactgttcg
     1441 tagtcagctt tttcatcgac aggaaggagc ttgtaagtta attcgtctgt ctcgttgtca
     1501 tgaatgatgt acatcttagc attctcttct ttgaagttac cgatttgtaa aatcgtatag
     1561 cgttcgtcga tcaattcgat atctttgatt gcttttgaaa aaactttacg gacatggtgc
     1621 attttctttt ggcggatttc tgccttttct gcagcttttg acaaagtaaa tccgtcatct
     1681 aaatattttt tgatcagttc tacttttatg actgtaggta aacgatattt tctaggacaa
     1741 ttcgcatcag cttcgatcga acgaatgaat cccttttgtt cccaatacct taattgacgt
     1801 ggagacacac ctacgatatc acttaattca ctgatgccaa caaccaattg atcattttcc
     1861 aacaaacgtt taagggaagc cccaaccatt tttgtcactc cttttgtcct cttgcttccc
     1921 ttaattatat ccgtattaac ttatgtgtca agtaggttga caaatagcga atttaggact
     1981 aaagtattct agtagtttca gaaaaaattc agaaagaagg agaaatatga gtaacaacca
     2041 acccgtagat atttacggaa aaccatataa tcggacactt ttagttgttg tcttattaat
     2101 tggtacattt tgtaccgttt tgaatcagac cttattgacg acagcttttc ctacattgat
     2161 gaaagctttc gatatttcag cgtctgatgt gcaatggtta acgactggtt tcttgctagt
     2221 caacgggatc atgatcccaa taacagcatg gctgatcaat aaattcagtt caagaaattt
     2281 atatctttct gccatgacga tctttttgat cggaacgatt acttgcttta cagcacctaa
     2341 ttttagtaca ttactaatcg gtcgtttgat ccaagcttgc ggtgtgggag tatcgatgcc
     2401 attattgcaa aatatcatgc tatctatttt cccgccagaa aaaagaggat cagcaatggg
     2461 gatgtcaggg atcgttattg gacttgctcc tgctttaggc cctacattat ctggttatat
     2521 cattgataac taccattggc gtgacttgtt tggtatggta ctgccaatcg ttgttcttgt
     2581 cttggtttta gcattcttcc taatgaaaag tgttattcca ttgtcaaatc ctagcattga
     2641 tattttatcc gctattttat caacgattgg attcggtagt ttactttacg gattttctag
     2701 tgtaggagat gacggctggg gaagcttgaa agtaattggt ttcttagttg ttggtgtgat
     2761 tttcatcgcc ttgttctcat ggcgtcaatt acatttggaa catccattct tggaacttcg
     2821 tgtattcaaa tcatctacat tcacgattgc agctgtttta gctggtgtaa caaatatggc
     2881 gatggtaggg gcagaaatgg tcgtaccatt gtatattcaa aatattcgtg gagaatcagc
     2941 attccattct ggcttgatgt tgttgccggg tgcattgatc atgggggcta tgatgcctgt
     3001 gacgggtgcg attttcgata aatacggcgc gaaacgttta gccatcacag ggatgttcat
     3061 tttgacagcg gcaactgcac catttgcttt cttgacaaaa acgactccag ttttatatat
     3121 cgtgatcctt tatgcgatcc gtatgtttgg gatctccatg gtcatgatgc cggtaacgac
     3181 ttctggtatg aatgcattgc caaataactt gatcagtcat gggacagcgg taaataatac
     3241 attccgtcaa atcgcaagct cgattggtac agctattttg atcagtgtgt tgacgaatgt
     3301 gacaaaagca aatatcccaa gcaaatctgt attggataat acgccattag cttataaaga
     3361 tcaggcaatc aatgccacac tgtcaggtta ccatgccgcg tttatcgtag cgatcatctt
     3421 tgggattctt ggctttatta ttgccttttt attgaagaaa aaagaacctg ttctaatgaa
     3481 agcgggggat aatgcatgat tattggagtt ttgattgtca gcttgcttgc ttttgcttta
     3541 acgaacgtgt tcgctaaaaa aacatggcaa acctttttat ctttgatttt cggactaatc
     3601 ttcgtcgctt cactaagttt gatcgtcgct aatttgtcca atcactttgg aatggaaaaa
     3661 gtaacggaaa caaaaacaga aaaaattgtt tccagtgctg attcccaagg agcagatatg
     3721 cttttgtata aagctttagg gaatggaaaa gaaaaagtgt atctttaccg aacaaatgaa
     3781 aaacaagaaa aaccaaaagc aactggtacg gataacgaaa caaataaagt agaaaagaca
     3841 gacggcgatg ctgaaaaagt gacgaaaact acttattggg aatacaaaaa cgacatgtat
     3901 aaattctggt tcaatattgc tgataacaat cacgaatatg acagccgagt gaatacattc
     3961 aaaatcccag agacatgggt agaattaagt acggaccaag cagccaagtt agctgaatta
     4021 gtgaagaaac agcaaagtac gatggaaagt gaagcaaaag cttatgttca agacggtatg
     4081 gtaaaagcaa tgactgaaaa tccaaaaatg agcaaagcag agcaagaaca acgtacgaaa
     4141 gccttagctg ctgaattcca acaacaagca ttcgcaaaat tagtgaaaga agcaaaaggc
     4201 gaataatatt ttgaacacca tgta
//
LOCUS       DACEMO010000188         4042 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEMO010000188 DACEMO010000000
VERSION     DACEMO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.194"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            180..776
                     /locus_tag="IW728_002384"
     CDS             180..776
                     /locus_tag="IW728_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1025722.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            900..2699
                     /locus_tag="IW728_002385"
     CDS             900..2699
                     /locus_tag="IW728_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1025723.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2977..3189)
                     /locus_tag="IW728_002386"
     CDS             complement(2977..3189)
                     /locus_tag="IW728_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025724.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1188 a          689 c          712 g         1453 t
ORIGIN      
        1 ttttaaaaaa aacaagttat taaataagtc tttagttgga tgtttttttg agatcaatct
       61 tttatactta tgatgaactt acaaaattgt ttgttttctg tcatatcaaa aaatggcgca
      121 gttagttgct tttctagtaa agtatagtta acaaagaacc tctaaggagt tgatgaataa
      181 tgaatattgc tgcctctcga aaactgataa atttcatatc cattatcgga ttaggcctat
      241 caatcgcgct gacgatttat tttattaatt taggtgtttt caaagatttg aatgcgttaa
      301 gaggattagt aggtgattcc atcattttag gaccgattat ttttgtcttc atccaaatcc
      361 tgcaagtagt tattccaatc attcctggag ggatcagtac tgcagctggt gtcctgatat
      421 ttggccctta tgctggattt atctataact atattggaat ctgcattggt tcgattatca
      481 tttttctgct agggcgtcgt tatggaaagc cattcatact ttccatgatt agcgataaga
      541 catataacaa atatattggc tggctggata atcaaaaccg ttttgagaag ctgtttgctc
      601 tagctatttt tcttccagtt gctcctgacg atgcgttatg tttgatggct ggattgacaa
      661 acatgtcagt gaaacgatat acactaatca ttttgattgc taaaccactg tcgattttct
      721 tatatagtat ggccttgatt tatggaggtc aatatctaag tggtcttctt ggataatatt
      781 tattttgtaa aattagtcat tgattgacaa tgttaaatct gggaaagggt ctgcaaaaca
      841 cactgcaccc tttccttttt cattagattt gcgctacaat gtgaagagca ggtgatagga
      901 tgaattattt gaaacgtagc ttaggtaatg cttgggactt tttaaaagga acacaagctt
      961 atttccgtga tgtcttgctg atgcatggct ttattttatt tatttgttta cccttattga
     1021 gcagtacgac ccgttttatt ttacaccgag gggcaattga ctatttgtct tatgacaata
     1081 ttccttcaat tttttctcaa catcctgggg ttctactctc gttaatcgta gttttgatga
     1141 cgattttact gttagtttat ttcgaattta cttttctttt gatgagtgtt ttttttatta
     1201 aaaagaaaga accaatctct ttgaagcaac tcttacactt gacgatactt cagctgaaga
     1261 aagtgagacc cattactttt ttgtttttcc ttgcttattt tttattgatt ctaccgatca
     1321 gcggattaag ttttaattct gatctactgt caaaaataaa aatccccgct ttcattatgg
     1381 attttatttt tgctaatcgt tggatcatcg tcagttcctt tttactcgtt tatattttcc
     1441 ttggctatat tgggattcga ctgatttttg ctttaccgga aatgattcta cgggatcgtc
     1501 cttttcgagc agccattcgg gaaagctggt cattgaccaa atcgcgtctt ttggccatca
     1561 cgggccaatt tattgtgatt ggcggtacga ttttactcct ttcttcttta ggatatatcg
     1621 ttgttatttt ggcacaaagt atggttgaac aatttttccc agactatgca ttgatcagcg
     1681 ctgtgtttgc tatgacgctt ttacaaggga ttttattatt caatatcgtg atgtctaccg
     1741 tagggatttt ttatatcatt gttgatttca tggatgacga aggcttcttg cctgagatcc
     1801 ctaagtggtt tatcccacaa gcccccaact tgcgtttctc tgcgctgaaa aacactggtt
     1861 tgactctttt tgcagttttt ttcggtattg gcgtctgtct ttacaatatg gattatctga
     1921 cttcagctgt tcagacaaaa ccagtgactg tctctcacag aggtgtgagt acccaaaatg
     1981 gggcgcaaaa tactttagaa gcacttgaaa aaacaagcag agactatcat cctgattatg
     2041 tagagatgga tgttcaggaa acaaaagatg gtcatttcgt agtgatgcat gatgcaaatc
     2101 ttcgtcactt aacaggggta aatggaacac cacaggatct tacattgaaa gaattgacaa
     2161 atcttacagt tactgaaaac ggagtgcaag cccctgtttg ttcttttgat tcttatttgg
     2221 aaaaagcaca taagctaaac caaaaattac ttattgagat caagacctct aaaaaagatt
     2281 cggatgacct tgtcgaacga tttgtccata aataccgcaa agttatctta gagaaccatc
     2341 atattttgca aagtttgacg taccaaaccg tttcagattt aaaagaagca gaaccaaact
     2401 tttatgtggg ctacatctta cctttcaata ttgttgggcc ccctgtgacg cctgctgatt
     2461 ttctgacaat ggaatatagt acgatcaacc gtaacttcat taattctgcc caccaagacg
     2521 gaaaagaagt atatgtatgg acaatcaacg attcagatgc tatgagtcgc atgatgtttt
     2581 atggtgtgga tggtatcata accgatgaga tgggactact aaatgaaagt gtgaaacaaa
     2641 cagatgaaga aatcacttat tcagataaac tgctaaattt tgttttggga gtcggctaat
     2701 caaaaatcgt taatgaaaaa agtctttata caacaaaaaa cgcataacct gccaccgcag
     2761 gtatatgcgt tttttgtttg acgctcataa tgtgtgctta actttctctt gattccttca
     2821 tcgcacgaac aatagctaaa atagcaggaa ttgcagagat cactgcaaga ataattccca
     2881 ttttccctga actttttctt tttggcatat cttctcgctc ccttctgtct ttatttatat
     2941 tataagggag ttcttgcgaa ttctcaaaaa tactgtttaa gaaaccaatt cagcaggtgc
     3001 gctaatatca cagaatttta cgagcatgcg atacttgctt tcattgataa gttgatagtc
     3061 tcgttggtca taactttcaa catacacgac agccgtgttg atatatgtac gttcaataca
     3121 gccgattagt tcatgagata ctccgatcgg tttgcatata taagatcttc ctacttctgc
     3181 ttttaccatt tcattccctc ttttctttat ttttattttg ttttttcttt attttataat
     3241 aacaaattac cttgtatttg aaaacgtttt tatccatttt tttatttttt attttgagta
     3301 tctttctgct ttttagataa ctatttttta acacgatctt agacgttgtg acaaaaacgt
     3361 ttgtttatat tcctatgttg ttcgtgcgtt tttttcttta tattgctaag ttatttataa
     3421 agaggtgagc atatgattta tattcaagca atagtatttg taaccttgat gattgggcta
     3481 cttatcggat taaaagaaaa tagctaaacc atttaagaaa aaataaataa ggaaaatgat
     3541 aaatgctttt taattgcatc aaaaaacgta cgatgctaaa cttggtataa gttcgaaagt
     3601 ttaaaggaaa ctttcaagaa ctaaattaca ttacaaagga gatgaatttt atgacgttta
     3661 caaaaacaaa tatgcaatac cgcgtagctt ttgatacaac ttctaaccgt tttatggcga
     3721 ttgatgcaca aaacgaaaat aacgttgcgt ttggtattac aatcgaacaa gcagtccgag
     3781 cattaaacga atctagataa ggagtgaacc attccaaatg gcgactgatg agatctcgga
     3841 aaatcaataa aagacgtttg taacggtaat ccgcgacaaa cgtctttttg tattcattac
     3901 atatatttgg aaattgtctc tctttatctc tagacctaaa caaaaacaca agttctaata
     3961 tcaaccatct cgttttttat gacaattttg ttagctgttt cgatgataat attccgttat
     4021 tatctgtata atatttaata ta
//
LOCUS       DACEMO010000189         4033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEMO010000189 DACEMO010000000
VERSION     DACEMO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.195"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(143..1648)
                     /locus_tag="IW728_002387"
     CDS             complement(143..1648)
                     /locus_tag="IW728_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1025725.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1645..2331)
                     /locus_tag="IW728_002388"
     CDS             complement(1645..2331)
                     /locus_tag="IW728_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1025726.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2524..3945)
                     /gene="gndA"
                     /locus_tag="IW728_002389"
     CDS             complement(2524..3945)
                     /gene="gndA"
                     /locus_tag="IW728_002389"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1025727.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1110 a          854 c          690 g         1379 t
ORIGIN      
        1 atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt tttttataat
       61 gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt ggatatataa
      121 aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga ataaagattc
      181 taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta tagttttcaa
      241 ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta gctcttgctt
      301 tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt attccttcgc
      361 caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag atatggactt
      421 cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt aattgttcaa
      481 agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct aaagtgatca
      541 catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact tcttttcctt
      601 tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag tctagcatct
      661 cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat gattcttcta
      721 atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct tcaataattg
      781 caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt tgttcaatat
      841 acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc aattcatcat
      901 ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata gtatccattg
      961 tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa cctaaaatac
     1021 tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc ttatttcgaa
     1081 tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt tctcttgtac
     1141 ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt gtaacgatcg
     1201 ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca
     1261 agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc tcagctataa
     1321 aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg acctcatctc
     1381 cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt tcttcatccg
     1441 agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt ctttcttttg
     1501 ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta aacacaacga
     1561 atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct ttcagttctc
     1621 ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc cgcgtacagt
     1681 ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac gaatatatac
     1741 gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta gtaaaacgtc
     1801 acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt attctctttt
     1861 tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa
     1921 atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa tatcgattcg
     1981 acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt tgacgatgta
     2041 gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc ttgcagtcat
     2101 cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta aaccgttcaa
     2161 ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg ccgcatctaa
     2221 accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca actctagctc
     2281 gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca tttattattt
     2341 tatcctttct attctactgg ctttttaaaa actctatcat tataccgata atatgcttaa
     2401 aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag tctactcata
     2461 acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac agccgaaaga
     2521 ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga cacgctcata
     2581 ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca gacgttctgc
     2641 tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta cgccagcttg
     2701 gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga tttcaacgaa
     2761 gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat ccgtaatttt
     2821 ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga tttcaccaaa
     2881 tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc cttgggcata
     2941 actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt cttttttgtc
     3001 tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc
     3061 tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa gtggcacacc
     3121 taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag ctgcatcaag
     3181 gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc
     3241 agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa agatatcagc
     3301 catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt cagcaatcag
     3361 ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc cggctccatt
     3421 tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa tttgctctaa
     3481 gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg aaggtccttt
     3541 taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc cagagttggc
     3601 taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt caatcaaaat
     3661 atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg ccggtcctgc
     3721 tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt cttcgattgt
     3781 atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg ttttagcccc
     3841 tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg ctagattttt
     3901 acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa ctcctcctac
     3961 atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat tgcaaactaa
     4021 agaacaacat att
//
LOCUS       DACEMO010000190         3943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEMO010000190 DACEMO010000000
VERSION     DACEMO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.196"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..346)
                     /locus_tag="IW728_002390"
     CDS             complement(<1..346)
                     /locus_tag="IW728_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1025728.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEAN"
     gene            complement(348..1787)
                     /locus_tag="IW728_002391"
     CDS             complement(348..1787)
                     /locus_tag="IW728_002391"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1025729.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            1972..3918
                     /locus_tag="IW728_002392"
     CDS             1972..3918
                     /locus_tag="IW728_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1025730.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1238 a          690 c          660 g         1355 t
ORIGIN      
        1 ggttggcttc aagcatttca atgtattctc tctctttagc aggatcattt tgactattgc
       61 aaataatcgt tttatatcca tgtttaaata actctatttc gagatactca atgagttcag
      121 agtaaaagat attactaata ttaggaaaaa taagaccaat aagctgaggt gactttcctt
      181 gaagtccacg tgctaaatta tttggtttat aaccaagagt tttcatagct tcatgaactt
      241 tttgttttgt tttttctgat aaatatccct tattattaat gacgcgtgaa acggttgttg
      301 ggctaactcc agctaattca gcgacatcag ttaattttgc taccatttta atattttaat
      361 tcaaagtatt ttccagtagg ttcgcctgat ttgatttgaa taccattttg gtgattttca
      421 gggaatacac gactagtaaa cactttttct cctttattaa taaaaatctc aatgattgag
      481 ttatctacaa aaatgttagc agttgtttct ttagtatcaa ttgagcattc acgaatcgta
      541 ccaaagtcag tggcatattg aatgcctgct ttgctgcgat ctaaaataat ttttccttct
      601 ttggtatcta caatcaagct aagtccattt cctttactat cagagaaaag aagtaactcc
      661 gatttctgat ttggaggaaa agttagttca agctcataag tattatttgt ttctgctttt
      721 gctgtaaatt gttcagaact agcacgtaat gatgtaatag cttctactgg atattggtaa
      781 agtttaccat cttttattga aagttctttt acaagactca ttgctccctg ataatcatac
      841 ttatcagttg gataatcgat atcaggtagt ccaatccaac tgacaatcag agcacgacca
      901 tcaggagtat tgaatccttg agtcgcatac gcttcaaatc cataatcaag attttgaatt
      961 tctgaagcgc ccacaagttt tactttttct atgtcaagcg attgacagat cttaaaagta
     1021 ttcggataga tattcccata atttagctca gatttatcca acccttgagg acaataaatg
     1081 agaacaggtt ttttgtctac aaatactaga tttggacact caatcatgta ttctgaacca
     1141 gtgccgccaa aatcaaggtt gccaacttcc tcccatgttt ccacgttatt gtcatccgct
     1201 ttgtaaagtt tgataaagcc tgatttttct aaattttgtg ctccaacaat cgcatacaat
     1261 tgtccttcat aattaaacat ttgtggatca cgaaaatgat cagttacatc agcaggttta
     1321 tgaataagaa cattctcacc tttcgtaatg ttgtagtctt tatccatcca tgctccgatt
     1381 tgaagtgtat cacgttccca attttcattt cgaacgtttc ctgtatagag taagaataga
     1441 ttcccctcta tttcatatgc agaaccggaa taagcgccgt ggctatctcg ttttgtgtct
     1501 ggaagaagtt ctgctcctgt tacatgaaaa tgaactaaat catctgattc attgtgtacc
     1561 cattctttca acccatgagc agctccaaat ggccaatttt gataaaaaag cgtatatttt
     1621 ccgttaaaaa aagaaaaacc attaggatcg tttaacaatc ctgttttagg ttcgatatga
     1681 tagctagcac gccaaggtga tcgttttaca ttatccttaa ttttatccat ctcatcttta
     1741 gtccaatcag aataagcacg atatcgtatt tctcgaggta agttcatatt ttactccttc
     1801 aatttatctt tttacaacta gtatggtaaa cgttttccgt tgaaaaatca atcatttcat
     1861 ttaaaaaaat aaactatatg acaaacgttt gacatatttt taagatatgc taaaataaat
     1921 ttgcatttca gtgaaccgtt ttcacgtaaa aataaaaaag gagtgattcg aatgaattac
     1981 agtaaagtag cttccgacgt aatacaagcg gtcggaaaag ataaccttat tgcagctgca
     2041 cactgcgcta cacgtcttcg tctcgtacta aaagatgaca cgaaagtgaa tcaaaaagct
     2101 ttagacgaaa atccagatgt gaaaagcaca tttaaaatta atggacaata tcaagtgatt
     2161 atcggtgcag gtgatgttaa tttcgtttat gacgaactta tcaaaaaaac tggactttcg
     2221 gaaatttcaa cagatgattt aaaacaaatt gctagtaaag ataaaaaatt caaccccatt
     2281 atggcattaa ttaaattatt atcagatatt tttgttccaa ttatacctgc ccttgttgca
     2341 ggtggtttat taatggcatt acaaaacttt ttaacagctg ctggactttt tggaccaaaa
     2401 tcaattgaag aaatgtatcc tgcgattgaa ggtatttcct caatgatcca actgatgtcc
     2461 gctgcaccat tcatgttctt accaatttta gtaggtattt cagctgctaa acgttttgga
     2521 gcaaaccaat ttcttggtgc tgcaattggt atgatcatga caacaccaga tcttggtggc
     2581 tcaactgaat attggaatat ttttggttat catgtttcac aaacaaacta tgcttatcaa
     2641 gttattccag ttttagcagc tgtttggata ttatctatat tagaaaaatt ctttcataaa
     2701 aaattgccat catctattga ctttacattc acgccattat tatcagtaat gattactgga
     2761 tttttgactt tcacagttat tggccctgta atgttattgt tgtctaatgg aattacagac
     2821 attattgttt ggctatataa cactactgga tttattggaa tgggtatttt tggaggaaca
     2881 tattcactta tagtcatgac tggtttacat caatcatttc cagctattga aactcaatta
     2941 ctttcagcgt ggacaaatgg tatcggacat ggagacttta tctttgtagt tgcatctatg
     3001 gcaaacgttg ctcaaggtgc tgcaacattt gccatttggt tcttaactaa aaattctaag
     3061 acaaaaagtc ttgcttcctc tgcgggtgtc tcagcacttc ttggaattac agagcctgcc
     3121 ttgttcggag ttaacttaaa ataccgcttt ccattctttt gtgctttaat tggttcagga
     3181 attgctacag cagtagcagg attacttaaa gtagttgctg tttcacttgg ttcagcaggt
     3241 ttcttgggat ttctttcaat taatgcaaca tctattccat tttacgtatt gtgtgaattg
     3301 attagctttg tagttgcttt tgcaattact tacttctacg gtaaaacaaa agctgctggt
     3361 atctttaaag cagaagaaat tgctgaacaa acaccagtta attcttcaaa agtagcaaca
     3421 acagatccaa tatctactga tgttgaatca acacaaacaa aagtttcatc agaaacaatt
     3481 gttagtccca tttctggtaa agtgattagt ctttcttcag taaatgatcc agtcttttca
     3541 tcagaagcta tgggaaaagg gattgccatt aaaccaaatg gaaacacggt ccattctcca
     3601 gtagatggta ctattcaact cacgtttgaa actggacatg cttatggtat taaatcaaat
     3661 aatggtgctg aagttctaat tcatattggt attgatactg tatcaatggg aggaaaagga
     3721 tttacacaaa aagttactac taatcaaaaa gttagaaaag gcgatattct tggagcattt
     3781 gaccgttcaa tgattgagaa agaaggtctt gatgatacaa caatgattat tgtgaccaat
     3841 acttcggatt attcagaagt aaatatagta gctgaaaata cagtcactga gggtactgat
     3901 ttacttgaaa tcaaataaat atatttccct gagtaaagat gga
//
LOCUS       DACEMO010000191         3913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEMO010000191 DACEMO010000000
VERSION     DACEMO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.197"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(563..1120)
                     /locus_tag="IW728_002393"
     CDS             complement(563..1120)
                     /locus_tag="IW728_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025731.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1299..1910)
                     /locus_tag="IW728_002394"
     CDS             complement(1299..1910)
                     /locus_tag="IW728_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025732.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2023..2280)
                     /locus_tag="IW728_002395"
     CDS             complement(2023..2280)
                     /locus_tag="IW728_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025733.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2877..3155)
                     /locus_tag="IW728_002396"
     CDS             complement(2877..3155)
                     /locus_tag="IW728_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025734.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3189..3440)
                     /locus_tag="IW728_002397"
     CDS             complement(3189..3440)
                     /locus_tag="IW728_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025735.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1191 a          797 c          519 g         1406 t
ORIGIN      
        1 taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga caagctaagc
       61 aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta actaattctt
      121 aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt acggtgattc
      181 tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt caaaaatagt
      241 tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg tcgtttattt
      301 taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag atggcaaaca
      361 caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca actattaact
      421 cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga tcacagccta
      481 gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc actggctgat
      541 gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga tcgtgttccc
      601 aactaataag acggattcca aacctatcgt acagatcttc taaaccactt ataaattttg
      661 cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta aatggatacc
      721 aattaggatt aggctctctt tttttctgta catctcccat agaatagtta gcactttctg
      781 ggacataata tattcttcga ccatcctcta attcatctaa taagatattt cgtgatcgtc
      841 tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct gcatctgcag
      901 aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc ccatctactt
      961 catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt ggcttaggac
     1021 ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct ataacaacaa
     1081 taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca tcacctctac
     1141 attctcacat attagttcgt aatactcctt taatatcaaa atatctactg gtcgtaataa
     1201 tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc ttttacttgc
     1261 tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa attgttaaca
     1321 atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa aacttcttgc
     1381 gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat aaagtctacc
     1441 tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa tgacattgat
     1501 tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat tcctagatcg
     1561 tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct tcgttctctt
     1621 ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt atcttcaatt
     1681 atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc ataggtgatt
     1741 tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa gcttggcaac
     1801 atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat aatttgtaca
     1861 attctcattg atccattgta ttccatttta ttccccctat tactattcat atccgcctat
     1921 ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt tactgagtta
     1981 cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa catgattaat
     2041 tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct cgacaacata
     2101 agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata aatttactgt
     2161 cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg cttcagcacc
     2221 accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga cagccctcac
     2281 cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac aaaggataca
     2341 atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa ataggcatta
     2401 attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta tctcatacat
     2461 tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat tttgtaattg
     2521 gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct cgtctatttg
     2581 tgattgacga aaaagaggca agacagacac tggaatggta attttaccca ctattttgct
     2641 ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt aattgatatg
     2701 ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc ttcttgggtt
     2761 ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag aaatgccgtt
     2821 tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga ctttgattaa
     2881 tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca atgttcgaac
     2941 tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga tgttatcaaa
     3001 gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat tttctgcagg
     3061 taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca ttgatcatcc
     3121 acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga aaagtttaag
     3181 gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt gagaattgag
     3241 aacaacaata agaatccata tcctcccgca aacatcccta aggctatact gtctctccat
     3301 ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa aaggaataat
     3361 ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa tgaccattta
     3421 tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg gaatgaattc
     3481 aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta tactttaaaa
     3541 aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat tagcaaataa
     3601 cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta gccaggatag
     3661 acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa gccttggaat
     3721 acagtagctc ttaattggta atactttttc cattataaat aacctctttt cattttatat
     3781 ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc cctcgctgtg
     3841 ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct gccagaataa
     3901 accaattatt tgt
//
LOCUS       DACEMO010000192         3880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEMO010000192 DACEMO010000000
VERSION     DACEMO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.198"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            237..1877
                     /locus_tag="IW728_002398"
     CDS             237..1877
                     /locus_tag="IW728_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1025736.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="IW728_002399"
     CDS             2031..3353
                     /locus_tag="IW728_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1025737.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="IW728_002400"
     CDS             complement(3415..3846)
                     /locus_tag="IW728_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1025738.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
BASE COUNT         1154 a          744 c          748 g         1234 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg
//
LOCUS       DACEMO010000193         3874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEMO010000193 DACEMO010000000
VERSION     DACEMO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.199"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..769
                     /gene="mvk"
                     /locus_tag="IW728_002401"
     CDS             <1..769
                     /gene="mvk"
                     /locus_tag="IW728_002401"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1025739.1"
                     /translation="LAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMGS
                     SAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFTK
                     GHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQA
                     KQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGGC
                     MIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            762..1739
                     /gene="mvaD"
                     /locus_tag="IW728_002402"
     CDS             762..1739
                     /gene="mvaD"
                     /locus_tag="IW728_002402"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1025740.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            1752..2837
                     /locus_tag="IW728_002403"
     CDS             1752..2837
                     /locus_tag="IW728_002403"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1025741.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            2834..>3874
                     /locus_tag="IW728_002404"
     CDS             2834..>3874
                     /locus_tag="IW728_002404"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1025742.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRT"
BASE COUNT         1205 a          809 c          840 g         1020 t
ORIGIN      
        1 tttagccgaa gctcctcatg cattaaaaaa tatcaaaaaa ttattcattc acttaaaaaa
       61 acagcatgac atccaaaaaa acttgcaact gaccattgaa agcacgattc ctgctgaacg
      121 tggaatggga tcaagcgctg cagtcgccac agcagtcact cgtgcttttt atgattactt
      181 agcatttcct ttgtctcgtg aaatactatt agaaaatgtc cagctttcgg aaaaaatcgc
      241 ccatggtaat cctagtggaa tcgatgcagc cgctactagc agcttgcagc cgatttattt
      301 tacaaaaggg catcctttcg actacttttc tttgaacatc gatgcttttt tgattgtcgc
      361 tgatacagga atcaaaggac aaacaagaga agccgtcaaa gatgtcgctc acctctttga
      421 aacccagcct catgaaactg gacaaatgat ccaaaaatta ggatacttga cgaagcaagc
      481 aaaacaagca atcattgaaa attcaccaga aacgttggca cagacaatgg atgaatcaca
      541 atcacttctg gaaaagctga caatcagcaa tgattttctt aatctattga tccaaacagc
      601 aaaagatacc ggggcattgg gtgccaaatt aactggcggt ggacgcggtg gctgtatgat
      661 tgcattagca caaacaaaaa caaaagccca agaaatcagt caagcacttg aagatgcggg
      721 tgctgctgaa acttggatac aaggattagg agtacatacc tatgtttaaa ggcaaagcac
      781 gcgcatatac caacattgct ctaatcaaat attggggtaa gaaaaatgaa gaactcatcc
      841 ttccaatgaa caacagcctt tcgttaacat tggatgcatt ttatacagag acagaagtca
      901 tcttttctga tagctatatg gtagatgaat tttatctaga tggcaccttg caagacgaaa
      961 aagcaacaaa aaaagtcagt cagtttcttg acctttttcg taaagaagcc ggcctctcac
     1021 taaaagcttc agtaatcagt cagaatttcg ttcctaccgc agctggatta gcctcctctg
     1081 ccagcgggct agctgcttta gcaggcgctt gcaatactgc tcttaagctt ggattagatg
     1141 atctctctct ttcaagattt gctcgacgcg ggtctggttc agcttgccga agtattttcg
     1201 gtggtttcgt cgaatgggaa aaaggccatg acgacttaag ttcttacgct aagccagtcc
     1261 cttccgattc tttcgaagac gatttagcaa tggttttcgt tttgatcaac gaccagaaaa
     1321 aagaagtgtc cagcagaaat gggatgcgtc ggacagtcga aacatccagt ttttatcaag
     1381 gctggttaga ttccgttgaa ggggatctat atcaattgaa acaagcaatc aaaacaaaag
     1441 atttccaact tctcggagaa acgatggaaa gaaacggact aaaaatgcat ggaacgactt
     1501 tagctgccca gcctccattc acttactggt ctcctgacag cttaaaagcg atggatgcgg
     1561 tgcgacaatt gagaaagcaa ggcattccat gctactttac gatggatgct ggccctaatg
     1621 tcaaagtttt agtggaaaac agccatttat ctgaggtaca agaaacattt accaaattat
     1681 ttagcaaaga acaagtaatt actgcacatg ctggtccagg aattgcgatt attgaataaa
     1741 gggagatttt tatgattgaa gtatctgcac caggcaaact ctatattgcc ggagaatatg
     1801 cagttgttga aacaggccat ccagcagtta tcgctgcagt cgatcaattc gtgacagtca
     1861 ctgtagaatc tgcacgaaaa gtcggaagta tccaatctgc acaatatagt gggatgcctg
     1921 tacgttggac aagacgcaac ggagaattag ttttagatat tcgggaaaat ccttttcatt
     1981 atatccttgc tgctattcgc ttgactgaaa agtatgcaca agaaaaaaac attcttttat
     2041 ccttttatga tctgaaagta acaagtgagt tagacagttc aaatggccgg aaatatggat
     2101 tgggttctag cggtgccgtg accgttgcaa cggtcaaagc cttgaatgtt ttttacgcgt
     2161 tgaatttatc tcagttggag attttcaaga ttgcagcact agccaattta gcagttcaag
     2221 ataatggttc ctgcggcgac atcgctgcta gctgttatgg tggctggatc gctttctcaa
     2281 ccttcgacca tccttggctc caagaacaag aaactcagca ttctatcagt gagttacttg
     2341 ccctggattg gccaggtcta tccattgagc cattgattgc tcctgaagat ttacgtttat
     2401 tgattggttg gacgggtagc cctgcctcta cttctgattt ggtcgatcaa gttcaccgtt
     2461 cgagagaaga taaaatggtg gcttatcagc ttttcttaaa aaatagtaca gaatgtgtca
     2521 acgaaatgat caaagggttt aaagaaaata atgtaacgtt gatccaacag atgattcgaa
     2581 aaaaccgaca attactgcat gatttatctg caatcactgg ggtcgtcatc gaaacgcctg
     2641 ctttgaacaa attgtgtaat ttagctgaac agtatgaagg agccgcaaaa tcttctggtg
     2701 caggtggggg cgattgcgga atcgtaattg ttgaccagaa atctggcatt cttcctttaa
     2761 tgagtgcatg ggaaaaagca gaaatcactc cactgccgtt acatgtctat agcgatcaaa
     2821 gaaaggaaaa ccgatgaatc gaaaagacga acatgtttca ctagctaaag cctttcatga
     2881 taaacaaaaa aacgaatttg acttcgtacg gatcatccat aaccctttgc cgcaaatagc
     2941 agtttctgat gtcgatctaa gtacacaggc agttgggttt acactaagca gcccatttta
     3001 tatcaatgcg atgactggcg gaagcgaaaa aacaaaaaaa atcaaccaag atcttgcaat
     3061 cgtcgcaaga gaagcagatt tgatgattgc cactggttca gtcagcgctg ccttgaaagt
     3121 cccttcttta gccgatactt atacgattat gcgacaagaa tatcctcacg ggaaaatcat
     3181 tgccaacatc ggtgcaggga cttctgtcga aagagccaaa gaagctatcc gattgtttca
     3241 cgcagatgcc ttacagattc atttgaatgc acctcaagag ttggttatgc ctgagggaga
     3301 ccgtgatttt accaattgga aagtacttat tcaagaaact caaacagcca ttgacgtccc
     3361 cctcatcgta aaagaagtcg gttttggcat gacaagagaa acgctcaatg atctagctgc
     3421 tttaggagtt catacagttg atatcagcgg tcgaagcgga acaagcttca cccagatcga
     3481 aaacgcccgt cgctccaagc gggaactgag ttatttagct gactgggggc aatcgacagt
     3541 ctcttcattg cttgaagcaa atgaagcaga cacttctatg gaaatcctag cctccggagg
     3601 tatccgcaat gcctatgaca tatttaaagc cctttgtctc ggcgcaaatg ccgtgggtac
     3661 ttcaggaacc gtactgacgc atctgatgaa tcacggcgta gaagaaacta ttatactgat
     3721 gaagcaatgg caagaagagc ttcgtctgct ttatactatg gtcggcgcaa cgaataccgc
     3781 ggccctacac caacaatctc tgatcttttc cggaccagtg aaggattggt gcgaagcaag
     3841 agggatcgac ttagtgaagt atggaagacg caca
//
LOCUS       DACEMO010000194         3819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEMO010000194 DACEMO010000000
VERSION     DACEMO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.200"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..651
                     /locus_tag="IW728_002405"
                     /pseudo
     CDS             1..651
                     /locus_tag="IW728_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            1399..3225
                     /gene="ltrA"
                     /locus_tag="IW728_002406"
     CDS             1399..3225
                     /gene="ltrA"
                     /locus_tag="IW728_002406"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1025743.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            3359..>3819
                     /locus_tag="IW728_002407"
     CDS             3359..>3819
                     /locus_tag="IW728_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1025744.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVS"
BASE COUNT         1302 a          665 c          863 g          989 t
ORIGIN      
        1 gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac cgttcgtaat
       61 aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa tgaaacgaat
      121 tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt ggatcagtca
      181 aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc agtggatgaa
      241 ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt aaaactagtc
      301 cggccctttg gcgatatgat ggggctacat gaagaattct tacccttaag taaacgatat
      361 atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg ttttggggct
      421 actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga tacaggtcgt
      481 aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt aaccaatgcg
      541 ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa caatctctta
      601 gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa agggggtgcg
      661 aaacgttcct acttgaaaag agtaggcaca gcacttcatt aaatcaatat ttaatgtgtg
      721 ttggagaact aaacacacga atagtcgaat gacggggtaa cgccctgaaa ggctacttta
      781 atactctgac tggcagaaac ttgagtaaac aaggctctgt cagaagctcg gtgaagtcgg
      841 cagacagaca ccgtaacaga taatgctgac gaaagattcc cagaggtggg cgttgtgtcc
      901 tatatgccgg aagtctataa aatatctatg gttagaatgt agatgagagg tctactgacg
      961 aatccttgaa tgtacaggtc tatatcgggg ctggatagaa atgtccagtg acacaaacag
     1021 tgtcgctact gtgggtgagt aagactccgc atttatgaaa atccatataa cgttaaaggc
     1081 gttatcaagg tagcaggctt atagaggaaa cctaaggata tatgtacaga tagtgtgttt
     1141 ggaacgttga aagctgacaa catggagatt ttactgtctg tgaagtgttc acatcagaaa
     1201 gttattgtga gattagcaat gatataaggt gtgttaatgt cagtgagagt ggtggcacag
     1261 tacctatgaa accgtgataa caagcggtgg agggatagcc actagtcata tgaagaatag
     1321 aattactaag aaaaatggta tgggttcgag taagactaag agatgtactt gctcctgcaa
     1381 acaaggaggg tacagaccat gctaaagaaa aacaagctcc gctataacga gtattatgat
     1441 atgcaacata tttatgacga gttatacgca cagagcaaga acggtaataa cttttataag
     1501 ttacttgaaa ttattggttc ggaacaaaat atttgtttag cttaccgtaa tctgaaatca
     1561 aacagtggca gtaaaacggc tggaacggat ggaatgacga tagacgatat aaaacaactc
     1621 agcaatgctg agattgttgc taccgttcgg gaaagcctta gcaactaccg ccccaaatca
     1681 gtcagacgtg ttttcattcc aaaagctggg agcgataaaa tgcgtccatt ggggattccg
     1741 tgtatctggg acaggctggt acaacaatgt atcctgcaag tcctagagcc gatatgcgaa
     1801 ccaaagttcc ataatcattc ctatggattc cgtgcgaatc gaagtgccca ccatgcagtc
     1861 agcagagtaa cgacactgat aaatttatca aaatatcatt actgtgttga cgtagacatt
     1921 aaaggatttt tcgataatgt caatcatgga aaactattaa aacaaatctg gacacttggt
     1981 attcgggata aaagattgat ttgcattatc agcaaaatgc tgaaagccga aattgacggc
     2041 gagggtgtcc cagaaaaagg aacgccacaa ggcggtctgt taagtccact gctctccctc
     2101 atagtcctta atgaattaga ctggtgggta agcagtcagt gggaaacatt ccagccgaag
     2161 aaccgcagta aaaatgggtg gcttcaatac gcaaagaaat ataccaaact gaaaagcggt
     2221 tttattgtac gttacgctga tgattttaaa atcatgtgtt caacctatgg agaagcacaa
     2281 agattttacc acagtaccgt agattttctt aataaaaggc tgaaattaga aatcagtcca
     2341 gagaaatcaa aggtagtaaa tctgaagaaa aattcatcag attttctggg gttcaaaatc
     2401 aaagtcattc caaaaggaag aacgaaacac ggttatgttg ccaaaacaga tatgaaccaa
     2461 aaagcattaa agaaagcgaa aacaaatcta aagttaaagg taaaagatat agccagacat
     2521 acgactggct tcaatatcag cagatataat ctgacagtta tcggtatgca gaattactac
     2581 tgcatagcaa ctaacgtata taacaacctg acagaagtaa gttacgctct cttaccgacc
     2641 atacgaatcc gtttaaggaa cattgcaaaa tcagttccat ttgaaagtac aagttcagaa
     2701 tttcagagca ggacaaaagg aatcagacca aaaacaaaaa tagtgatgat tgcggataat
     2761 ccgctattgc cgattcaagg agtacaacac aaaaatccta tgaatttctc gcaagatatt
     2821 tgtaatttta caaagcaagg gagaaacaag gtgcatgagg acgttgtagt tgtaacaaaa
     2881 gaagaaatcc gagcgttgtt ggaaaatgaa aaccctgccg atagcgtaga gtttaacgac
     2941 aatcgtattt cggcatatat cgcacaacaa ggcaactgct atgtaatgaa tcgtcgagga
     3001 actccgtcca ctctgatatg tattcacaaa tcagacaaag gagataacct tgacagatat
     3061 tctaatctgg catttgtgga aattcccatt gcaaaggcaa tcctaacaga atcagcagac
     3121 gaagcaaaat cactgttaaa aggttatgtt cttaatagtc agcagaagaa aaaactgaac
     3181 agaatacgga cgaattacgg ataccagaca ataactggga aataacttat aaatctattt
     3241 ggatatgatg gaacgccgta tgaagcgaaa gtttcatgta cggtgtgaag cgggggaaaa
     3301 cttggagata acatcaaaaa gttacctatc gctatagcga ggagggtgga aagaaacctt
     3361 gccagaaatt gcggatgaaa tcagtatcct caacttgacg agtaagtcag aaaatcaagg
     3421 tctgcttgat ccttatgtga ttatgaagaa aaagaaagac tcggaaagtt tagcaattga
     3481 tattctgact tttttaacag gaatttcaag tcgagacggg gagaagtttc ctgtgttacg
     3541 taaagccatt cgaacggtga ctcaaagtga tcaacggggg ttattacaag ttattaacga
     3601 attgcgaaaa gacgggtcac caatcgcgga aaatatcgcc gatcatatcg agtcctttac
     3661 cgattatgat tttgctcatt tgcttttctc agacggctcg attacccaat cgattagttt
     3721 agataaacag ctaaatatca ttcaagtggc ggatttggtt ttaccggatg cagaaacgac
     3781 ttttgaagaa tataccacca tggaattgct tagtgtttc
//
LOCUS       DACEMO010000195         3817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEMO010000195 DACEMO010000000
VERSION     DACEMO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.201"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            352..1041
                     /locus_tag="IW728_002408"
     CDS             352..1041
                     /locus_tag="IW728_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1025745.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IW728_002409"
     CDS             1028..2236
                     /locus_tag="IW728_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1025746.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IW728_002410"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IW728_002410"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1025747.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1258 a          658 c          771 g         1130 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaag
//
LOCUS       DACEMO010000196         3800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEMO010000196 DACEMO010000000
VERSION     DACEMO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.202"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            52..273
                     /locus_tag="IW728_002411"
     CDS             52..273
                     /locus_tag="IW728_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025748.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            286..786
                     /locus_tag="IW728_002412"
     CDS             286..786
                     /locus_tag="IW728_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1025749.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            883..2730
                     /locus_tag="IW728_002413"
     CDS             883..2730
                     /locus_tag="IW728_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ1025750.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2733..3629
                     /locus_tag="IW728_002414"
     CDS             2733..3629
                     /locus_tag="IW728_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1025751.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3679..>3800
                     /locus_tag="IW728_002415"
     CDS             3679..>3800
                     /locus_tag="IW728_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1025752.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLS"
BASE COUNT         1343 a          496 c          803 g         1158 t
ORIGIN      
        1 atgcaagtgc agcggcggac cgagcaatgg aaaagaaagg aaagtgatga aatgaatttc
       61 ggtcaaaatt tgtatcaatg gtttttaacc aatgctcaat ctttagtctt actagcgatt
      121 gtggtcattg gtttgttttt aggatttaaa cgcgagtttt ccaaactgat tggcttcttg
      181 gtgattgctt tgattgcggt aggtctggtc tttaatgctt ctggtgtgaa ggatgtctta
      241 ctcaatttgt ttaaccgaat tgtcggtgcc tgagggggaa tcacaatgga acagatacgt
      301 gtttatattg cgaaccttgg aaaatacaat gaaggtgagt tggtgggggc atggtttacc
      361 ccacccgtcg attttgatga agtgaaagaa cggattggat taaatgacga atatgaagag
      421 tacgcaattc atgattatga actgcctttt gagattgatg agtatacacc aattgaggaa
      481 attaatcggt tgtgtggttt agcggaagag cttaaaggaa cgccaattgg tgaggtcgct
      541 tcagagattc aacatgcctt cttcaattct ttcgaggaaa tggtagagca tgtggatgat
      601 atcatctatt atcccgattg taatgacatg agtgatgtag cgtattactt gattgtagaa
      661 gctggtgatt taggcgaagt accagaacac ttgaagacgt atattgattt tgatgcgtgt
      721 ggtcgagact tggagattgg cgggaattat ttggttacga gtcgaggggt ttttgaacta
      781 atgtaaatca tttgttagaa ttgaccagaa atttctatcg tacttttact tttctatttg
      841 atatactgaa atagaaaata tttgttgata ggggtgttac taatggttgg caataagtgg
      901 tacaaatgtg atcttcatat tcattcaact gcgagtcagt gctttcagga taaaaaggtt
      961 actgctaagg aatgggtaga agaatgttta aacaaaggac tcgattgtgt tgcattaact
     1021 gatcacaata caggggaaaa tattgatgaa tataagaaag tggctatcga aaatggctta
     1081 gatttttttc ctggagtgga attaacgtgt ggagaaaatg gaactcattt actagtccta
     1141 tttgataaaa gtgcatgtac aaatgatatc tctgatttct taaccaagat gggtataaaa
     1201 agagaggatt gggccagcgc tgcagcttgc tcaatgaaat cagttttaga agttattgat
     1261 tttgctgagc acgagggctt gctcgttatt cctgcacata tagatgagtt taatggaatt
     1321 tcttcagcaa aatttggaaa tctaggaaag atatttgaat cagaaaatat atatgcagta
     1381 cagacggttc aaaaagaatt ttttgaaaat cgctcacaaa tgattccctc atctaagcgt
     1441 gacactatct atgatagtgt gaatgccaga tatgatggta gagttggaaa agatactttg
     1501 gaatcttggt ataaatcagt tgttgaggca gaaaaaaata atatgacatt tcttagcttt
     1561 tctgataacc cacactcgaa aggaaattca aaacatggac tttggggaat aggcacaaga
     1621 tattcctata taaaaatgaa ggacgaacct gacttgggat cacttcgcga tgctttaatg
     1681 ctaggcgaaa ctcgagttca ttctgatttt tctaatttct ccattaatga aaatgaagtt
     1741 ttattggaaa aattaagctt ttctgggacc actcttagca caaaagaagt tgtagtcgaa
     1801 ttcagcgata atttaacttc aattattgga ggaagaggaa caggcaaatc ttgtattaca
     1861 agattcttgg tctatgtcct tggaaaggaa gcagaacttg atcaattttc tgaaatacaa
     1921 agtgattatc agaactttgc acaaattgaa cataatggct ctggaatatt tttgaaggat
     1981 acaattgtga aactgaatat tttctataag gggacaaaat atcaaataat aagaacacag
     2041 gatagacatt ctatttatga atttacacaa gagaatggtc tggttgaaaa agaaacagaa
     2101 cggttgagaa tgatttccga taaggtgagt atatactctc aaaaacaaat ttatgaaatt
     2161 tcaaaaaatc aaactagtat actagaatta ctagatggat ataactctga tttgatttca
     2221 gagtataaaa atgagattga aacatgtgta aacgaaataa gaaaattaaa ttgggatatt
     2281 gtttctgtga aaaaagaaat acaagataag gcgaaggtag agcttgaaat tgaagattta
     2341 aaaaaacaag tagaaaagct atctcataag agttataaag atgtttatga tgagtattca
     2401 aaagagacag atatttatag ggaactgaaa cgtgatgtgg aggcacttaa ggaaattccc
     2461 aaaaatctaa ctgatagtgt tgacaaaatt gactttggca atggtataaa agttacagac
     2521 gaaattgatg aacaccgcgg agaattgatt aagaatttag tgaccaatcg ggctaagatt
     2581 caaacaataa ttaatgaaat gtcagatgaa attgatagtt atagagcaga gctgaataaa
     2641 tcagattgga aagttaacta taatgaggtt tcaaagaggt atcaaagagg tatcaaaaac
     2701 ttagtgaaga gttatctcaa gatgaattaa aaatgttaca aaaccttgat tctttatatc
     2761 aatctttatc aaaaactgaa gagcgattgg aagaaattat tcaacaagaa ataaagctta
     2821 aagattacca agttgaggtt gactcagcaa tgtcaagaat gaataaatca tatcaaaatt
     2881 tctattctga gagaagtagt tcctcgcgcg aaattttttc tgatattaca ggaatagagg
     2941 ctactattca accaaatagg gatttcaata attatgttaa agagctcaga gcttgtatta
     3001 ataaggagga cacttttgac agtgagtttg gatctcttat tgataagctg acaaaaaatt
     3061 ccattactaa tcaagatata tataatgaca tacttgatat aagagttagt ggggaaagta
     3121 gtattttttc agataaaaaa ttaatcaatt ctataagaaa attgaatgat gcacaagttc
     3181 ttgaattaca aacccttaag ccaaatgatt tagtagttat ccgacttgaa ctaaatggaa
     3241 aaaaagttag tttatcgaat gctagtgctg gtcaaaagac atctgcaatt ctttctatga
     3301 ttttggcgta tggaaattca ccactcatat tggatcagcc tgaagatgat ttggatagtc
     3361 aattaattaa taacttaatc gtaagaagta tcattacaaa aaaggaaaac agacaaattt
     3421 taattgtaac tcataatgca aatatcccag ttaatggtga ttcagaatgg ttgattagca
     3481 tgggagattc aaaagaaata tcggctgatc agtgcgggtc ggtagatgaa cccaatatca
     3541 agccaagaat atgtactgtg atggaaggtg gcgaggatgc ttttacaaat agagcgaaac
     3601 gctatggttt taaacaaatt gtagaataac actagagggt gagacatatt attatgtccg
     3661 ccctttttag gaggaaaaat gaaaaaaata aaaagctata ccagtatctg ggcagttgaa
     3721 aaagtcattt acgcaatcaa tgattttcag ttgccttttc ctgtgacttt taaccaaatg
     3781 gcttggttcg ttttatcttt
//
LOCUS       DACEMO010000197         3756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEMO010000197 DACEMO010000000
VERSION     DACEMO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.203"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(337..1584)
                     /locus_tag="IW728_002416"
     CDS             complement(337..1584)
                     /locus_tag="IW728_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HAQ1025753.1"
                     /translation="MKNKLTLLICATILSTWAVPTQNAFAEETGNDTTQQTVTSNSSS
                     EEQTAIVNGEEVKLEAPVKEGQSEAEATQAPKEELQQDQMSRFARSAVRTIPMYRMYN
                     PNSGEHFYTQDTGERDNLRRIGWNYEGIGWQAPTSGDPVYRLYNRYNGEHFYTLNAKE
                     RDNITKQGWTYEGIGWYSYKGANGIPVSRLYNKRVNWHHYTLDEHEKNVISKQGWNYE
                     GVGWYAVGNGQPSNDDYKLLGVKNYNQYALGAPSGCEGASLLQGLQYKGKITNWSLTQ
                     FLNTIPKSPNGNPNNGFVGSPFVENSWTYSAIYPAPLATWGQKYGNVQNISGSSMNTL
                     LNEVKNGNPVVAWVTINFQPIRWGNWSFGVAANNNHAVTLDGYNKGSNQVHVSDPISG
                     SYWLNRTTFENIYNARKYAVVVR"
     gene            complement(1852..2475)
                     /locus_tag="IW728_002417"
     CDS             complement(1852..2475)
                     /locus_tag="IW728_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1025754.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQKLISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
     gene            complement(2629..>3756)
                     /locus_tag="IW728_002418"
     CDS             complement(2629..>3756)
                     /locus_tag="IW728_002418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025755.1"
                     /translation="SLLKNAYYWIISKENEKESYRNSKRYQYLNSMTGNIANQITIWD
                     TIWEDAEQRIGLDELKLLKIFFKHCKVAEGQNKLNLLNTYLRDYEEDTFFKVTYSNYE
                     IIQDYIYHDLSIILNDSLSEEFGDYDLLVVIRNMFFKFTKQCYNASRIIIYKYNSKFN
                     SFFISSDSRILNRLFLNNFDIRFIETILNSRFTNDISLKENLPSCFNYGNSNLTAEHQ
                     NIIVSMFSKYFDNYLNSADHYDVEKVLLLNELLKVVFNKVDLSLYITMENFRNYYSKK
                     IHNLLDLDDDSFMINFFKEYALMGDCVSTISENLFKDTEWTRNLLMSIYISRYPIFTD
                     EDRLNKLSQGISSTIESVELDTKGQKKLNYLKQKIDMLINL"
BASE COUNT         1176 a          677 c          506 g         1397 t
ORIGIN      
        1 gcataaaaag acttcatgtc gattagaaaa taatcattta ctggttcttt cgaataatca
       61 aacatgaata acaccccttc tttttacatt atacaaacgt atgttcgata ttgtaaagaa
      121 gaaatagcta aacacacaaa aaaagaagcg taggtaaact cttaccctgc ttcttctttt
      181 gatttgtatg atcaactcat tgtatcactt tattaaataa tttgaaaatt taacaaaaac
      241 tctcactcta aatgagtgag agactgcaaa ttagagtacg gaatttttgg gagttccgtt
      301 ccttaatatt aacaaatgat cttgaagcat acaatgttat ctgactacaa ctgcatattt
      361 tcgtgcgttg taaatatttt caaatgttgt tcgattcagc caataagaac cactaattgg
      421 atcagataca tgtacttgat tacttccttt attgtatcca tcaagtgtca ctgcgtgatt
      481 attattagct gctacaccaa aactccaatt tccccaacga attggttgga aattgatagt
      541 tacccatgca accacaggat ttccgttctt tacttcatta agcaatgtat tcatagaact
      601 acctgaaata ttctgcacat ttccatattt ttgcccccaa gttgctaatg gagcaggata
      661 aatagctgag taagtccaag aattttcaac gaatggacta ccaacaaacc cattgtttgg
      721 attaccattt ggagatttag gaatcgtgtt taaaaattga gttaaactcc aatttgtgat
      781 ctttccttta tactgtaatc cttgtagcaa tgaagcccct tcacagccac ttggagcgcc
      841 taaagcatat tgattgtaat tctttacgcc aagaagctta tagtcgtcat tgcttggttg
      901 tccattgccg actgcgtacc aacctacgcc ttcataattc caaccttgct tactaatcac
      961 gtttttctca tgttcatcta gtgtgtagtg gtgccaattt actcgtttat tatataagcg
     1021 actaactgga ataccattag cacctttata agagtaccag ccaattcctt catacgtcca
     1081 cccttgtttt gtgatattat cacgttcttt ggcatttaaa gtatagaaat gttccccgtt
     1141 atagcgatta tataagcgat aaactggatc gccacttgtt ggagcttgcc aaccaatacc
     1201 ttcgtaattc caaccaattc ttcttagatt atctcgttct ccagtatctt gagtgtagaa
     1261 gtgttctccg ctatttggat tatacatgcg atacataggt attgttctta ctgcactacg
     1321 agcaaaccga ctcatttgat cttgttgtaa ctcttctttt ggtgcttgag tcgcttcagc
     1381 ttcagactgt ccttctttca ctggcgcttc taattttact tcttctccat ttacaattgc
     1441 tgtttgctct tcgctagaac tattgctagt cactgtttgc tgcgtagtat cattccctgt
     1501 ttcttctgca aaagcatttt gtgtagggac tgcccacgtt gacaaaatgg ttgcacaaat
     1561 taataatgtc agtttattct tcatttttat ttagtcctcc atctctttat taaaagtgta
     1621 acgatcgtcc ttttagggac ttgatattga caataaaacg atatttgctt ttctttaaat
     1681 atttactttg cattaatgta caacgtacga ctctcactaa aacacagttg tcttttagca
     1741 tttgtaaaac ttgaacttca aaagaacctt ccaaactagg aacttcgcaa taataaattt
     1801 taccgatttc tatttcttgc attatttacg cttctttcta actggaactt attacattcc
     1861 taaatctctt tttactcgat aaacagtttt tcttgtaacc cctgcattct ttgctatttt
     1921 agaaatagtt tcttttttct tcagaccatc aacaatttca tcataaatca atttgttttt
     1981 tcctgtcgca ttggcatggt attgaatctt tttaccagta taacgaccat ttttctttgc
     2041 aatttcaatg ccttgttttt gacgctcttt gatttttttc cgttcgtttt cagcaacata
     2101 gctaaacact tctatgacta aatttgttag caagtctctt aatgcagggt cagttacccc
     2161 attaaacgac ggtaggtcta atatttcaat agaagcacct ttactacgaa acaattgaag
     2221 taagtctttt gtatctttac tatttcttgc taatcgatca agactaggaa taataatcgt
     2281 atctcgttca cgtatgaaag aaataagctt ttgcagctct ggtctgtttt caatattttt
     2341 tccagagact ttttcctcga aaatttttaa atcgtcttca gcaatattat ttttgactga
     2401 ccagttttca attagcttat gttgccgagc taaactttga tcgtcggaag aaactcttaa
     2461 ataagctaaa tacatttgcc atcattccta tccaatctat ttactattag tatacaggtc
     2521 ttagggtaca ctttgcaata aagaaaaaac gctgaaaaat caacgcttta agcaaatatg
     2581 gatttgtacc attagagtat actctaatgg tacaaaaaca ctaattgttt ataaattgat
     2641 caacatatca attttttgtt ttaagtaatt taattttttc tgtccttttg tgtccaattc
     2701 aacactttct attgtagaag aaataccttg acttaactta tttaatcgat cttcatctgt
     2761 aaaaatggga tatcgagaaa tatatatact cattaacaaa ttcctagtcc actcagtatc
     2821 tttaaataga ttttcggaaa tagtagatac acaatctccc ataagtgcat attctttgaa
     2881 gaaatttatc ataaaagagt catcatcaag atcaagaagg ttatgaattt tcttagaata
     2941 gtaatttcta aaattttcca ttgtaatata taaagataaa tctactttgt taaagactac
     3001 tttcaatagt tcgttaagaa gaagtacttt ttcaacatca taatgatcag cactatttaa
     3061 ataattatca aaatatttac taaacattga aactattatg ttctggtgtt cagcagttaa
     3121 attgctattc ccatagttaa aacaggatgg taaattttct tttaggctta tatcattagt
     3181 aaatctacta tttaaaatag tttcaataaa tcttatgtca aaattattta aaaatagtct
     3241 atttagtatt cgagagtcag aactaatgaa aaaagaatta aatttactat tatatttata
     3301 tataattatt cttgaagcat tatagcattg ttttgtaaat ttaaaaaaca tgtttctaat
     3361 aacaacaagt aaatcataat ctccaaattc ttcagataaa gagtcattta atattatgga
     3421 taaatcatgg taaatataat cttgaattat ttcataattg ctataagtaa ctttaaaaaa
     3481 agtgtcttct tcataatctc gtaaatacgt atttaacaaa ttcaacttgt tctgaccttc
     3541 tgctacttta cagtgtttaa aaaaaatttt cagaagtttc aactcgtcta atcctattcg
     3601 ttgttcagca tcttcccaaa ttgtatccca gatagttatt tgattagcta tattaccagt
     3661 catactattt aaatattgat atcttttgga attacgataa gattcttttt cattttcttt
     3721 acttataatc caatagtatg cattttttaa aagtga
//
LOCUS       DACEMO010000198         3744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEMO010000198 DACEMO010000000
VERSION     DACEMO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.204"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(616..1317)
                     /locus_tag="IW728_002419"
     CDS             complement(616..1317)
                     /locus_tag="IW728_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1025756.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(1333..1773)
                     /locus_tag="IW728_002420"
     CDS             complement(1333..1773)
                     /locus_tag="IW728_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1025757.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1773..2348)
                     /locus_tag="IW728_002421"
     CDS             complement(1773..2348)
                     /locus_tag="IW728_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1025758.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(3196..3675)
                     /locus_tag="IW728_002422"
                     /pseudo
     CDS             complement(3196..3675)
                     /locus_tag="IW728_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         1131 a          769 c          588 g         1256 t
ORIGIN      
        1 agatcactac caaagatgac gaatatatta tttcaggaaa agaaaataaa aaagaaacaa
       61 cagtcatatt tacaaaaatc ggtaattacc gtttgaagga tgaggatggc aacatctaca
      121 gtctatagtg ccgtctgttg gatcttcttt tgtttctccc cttcttttct gctatagtaa
      181 agaagcaaga tgattgtaaa ggagacgaaa tggatgatcg atagtcctga ttattgtaca
      241 tgtaataaca acaaaaatat ccatacagaa tttaatgacc aaggaagctt gagcatatgc
      301 gacaactgtc acaaactagt caaagattca tttaaaacat ttgaatctta tcctgttcaa
      361 aattatgtaa aaagtagaag aagataaaca aatccaagca aaactcacta tggctgattt
      421 ttatgaactt caaactcagc ttcttccttt gatcaagctt catcagtaaa acaaacagtg
      481 gatcatttca gtttacaaat caaagagtaa agatttattt atttcaaata tttgaatata
      541 aaataaccaa acgatagaaa ggcttattct acatcacgta gtaatttttc tttcccgtcg
      601 tttggttttt tatttttagc ctaattcttg accttttgca tgctggttat ccgtaaaata
      661 ttttccagca ggctttcctt ccaattcagc taatttttct tggatctgac ggatcacctt
      721 ttttgcatct tctggatttt ctacaaaatc gaatttatcc ccatccactt gtattttagg
      781 acatacatca aaatcttcat accattgatt atagcgggtg ttcaacgttt cataataggt
      841 atataacgtc tcatcgcttt ctagctgttc atagtcacgc ccacgttttt tgatacgttc
      901 tagcatcgtg tccaaagaaa ctttgatatg gaccatcaaa tcaggccgtt tttttggagc
      961 aacatcatct aactcgttta gcatcgtatc taataagcta tcgtactgct gtacttcgat
     1021 atccgttacc cgacctaaat cagcgtttag atggaacaac aggctatctt catagatgga
     1081 tcggtctagc acattgtcat cgttcactaa agcatttttc attgctaaaa atcgcttgtt
     1141 caaaaagaag atttgcaata aaaaagcgta ctgctcagga tttgaataaa agagcggcaa
     1201 tacttcgtta tcttcgacat tttcataaaa tggtttagaa tcaatctctt ttgcaagcat
     1261 atccgtcaaa ctgctcttac ctgcaccaat cgttcctgaa atcaaaatca ctgacataca
     1321 acccttcctc ttctataagt actcgcctac gtaagtttca acaggcaatg tttcaaaatc
     1381 atattcacgt acagcttggt ctgattttaa atacgtatgg agactttgtg taatcatcaa
     1441 gttcgtaggt accgtctctt cttgtacaat gatcatcggt tttttcaaca tagttgccac
     1501 acctaattcg taagccgttc ctggatcgat tgtttgatgc tcaaagtcaa ggatagcaac
     1561 aacaaattct gcatttgtca gttcttccat atctttttcg tatacttcct gcgcccatcc
     1621 agcgctgaat agttcataat tgctttcttg gtgatgacgt ggagagtaga aacttgtgac
     1681 tgttttgttt tcctccaacg ctttttcgat acgacttact cgatcgattt gctcctctga
     1741 aaaaaatggt cctgctaaat atatttgact cattattttg cctccttcaa taataactga
     1801 tttttaatag atactgctcg taattcattg accagttcca atggattgtt ggaaaagatt
     1861 acttgatcca accatttttt atccataaaa tcctcttttg caaaagtttc taataattgt
     1921 tgcaagccgc tgtaataacc gccaatattc aatattccga ttggcttgtc gggcgtcaga
     1981 ccgattgcta tccaagaaag aagttgtgag aattcctcca atgtacctag acctccaggg
     2041 aaaacgagga cagcatctcc cttttcaaat aataatacct ttctttcatc gatcgtttct
     2101 gtaggaataa atgtagtaac atcttttcca ggcaattcag tttcaaaaag cccattcgga
     2161 tagatacctg tgacttttcc gttattttcc agtactgttt tactaatcac tcccataatc
     2221 ccggagtatg aaccaccgta aaccagttcg atttcttctt gtgccatgta ttttcctaac
     2281 gtttgcgcaa taaatttgta actttcttta ttaccaaaac gggaaccgca aaaaacagta
     2341 agtttcataa aaaaacactc cctaactcaa aagtctatct tcattctaca cgtgtttttt
     2401 taaaataaaa gactagattt tgtagttttt tagtggtata taggatagac gccactatat
     2461 atagtattta gaaaaaaatc atttttcagc cattttatag ataaaacaaa aataaataac
     2521 gtgttttcat cgatatgaaa tgacttactt atagaagata ctgggatttc gtcctatatg
     2581 ccaataacgt gtattatttt aaacaacaaa tacttgtttc ttttattaac gaaaaaatca
     2641 ttttgttctc gtgttatgtt tcactgataa atgatgtttc tctaaataaa aaagccgacg
     2701 aactaaacga tcaaaatttg atcatttttg ttcatcagct tctcctaaaa atctttttgt
     2761 taacgataaa tagctggatt tgtaaaaata tcacttgaat cgtcactgct ggaagaaaat
     2821 tcagtgataa ccgcacctgt tgcgcctgcc tggaactagt gtttgatatt gggctcgaac
     2881 gtgtactgat ctctagcatg aagaataatt tcttttttta ctgtataata actttctttc
     2941 ctttttggaa tagtacagga aagttttttt gtttcctttc cttcgacata aggtatacca
     3001 gaccctttcg acaacgaaag gttctcattt ttccttcatc gtcatttcaa ggagggtgcc
     3061 gatgctctgg acagtttcga tcgctcctaa accaaaatct gtatattcga ttctttctaa
     3121 ctctgattct ttcataccat tcctgtctca aggtacatct attgtacttt tttcttgtaa
     3181 tccattctgc ctctcctaac taattgtcaa atcatcccaa gatatgtctt cttcctcttt
     3241 ttcttcttgg atgatttctg cattcggatc tactggtttt ttcaataata aaatggttat
     3301 tcctgtgaca aaagaaccag ctaacaatgc accaatgaat gcccatggtc tggtcatcgt
     3361 agcgactgca aacattcccg atgccggaat acggctatct gcgccccaaa acatgcttaa
     3421 tccgccacct actgtcccac cgactcccgt tgctatgaca ttgcgtgcca agtcgttcaa
     3481 tacgataggg aaacatccct ctgtgatctg acagatcccc ataggaaaag cggtcttcaa
     3541 tgtatcgatt tcttgacgat tgagtatatt tttacgaatg attttcccta agaaataagc
     3601 gatcgtcaag ccgaatgggg ttaccatcga agctaaaatc aatacagtga ttggttcata
     3661 gattccttct gaaaatcgtg ccacaccccc ttatccagaa taaatggtaa tccagaaaca
     3721 gccgtgtatc tgcggtaata agcc
//
LOCUS       DACEMO010000199         3738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEMO010000199 DACEMO010000000
VERSION     DACEMO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.205"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            596..1924
                     /locus_tag="IW728_002423"
     CDS             596..1924
                     /locus_tag="IW728_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1025759.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2126..2959
                     /locus_tag="IW728_002424"
     CDS             2126..2959
                     /locus_tag="IW728_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1025760.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2975..3712
                     /locus_tag="IW728_002425"
     CDS             2975..3712
                     /locus_tag="IW728_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1025761.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
BASE COUNT         1252 a          585 c          780 g         1121 t
ORIGIN      
        1 aaataaagct tatgcaaaga aaaagaaatc caatcacttg ggtttctttt tcttttatta
       61 ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac gaaagagtag
      121 agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa taaatcagca
      181 ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc ccaaagttat
      241 ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc aggtttttca
      301 ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt attttgaaag
      361 ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc attttcgtta
      421 tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac atttttcttc
      481 atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga gattgtattt
      541 ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg attggatgaa
      601 agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa atatcaaaca
      661 gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg aaaatgtttc
      721 agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga aagtggctaa
      781 tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc gtcataagat
      841 gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt ttttctggga
      901 taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa aagaggcaat
      961 cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg gccaatggga
     1021 tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga tgccggaaag
     1081 tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac tgcgtaaaga
     1141 tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta ttgaagaaaa
     1201 gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag gaacacctcc
     1261 aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg accgaggact
     1321 gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt atgttagcgt
     1381 gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag agatgctagg
     1441 aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa ctttcaaaaa
     1501 attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg atgtaggtca
     1561 atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg atgatctatt
     1621 tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa gtctgatgac
     1681 ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa aatggaaagt
     1741 cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga gtgatgcctg
     1801 gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga aagagcaatt
     1861 aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg gtgctttagc
     1921 atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg cttaagccag
     1981 ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta taattctaaa
     2041 taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac gaagtagaat
     2101 aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg atctgaaatt
     2161 aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc gtataaaaac
     2221 cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat tttttgaagc
     2281 agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata cgtatatgct
     2341 aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg gaaaagggga
     2401 tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc cacaatttgg
     2461 ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg aagaagattg
     2521 gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag aagaaacgaa
     2581 tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg tattgaaatc
     2641 ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga gtggtaatgt
     2701 gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt atgggaatcg
     2761 ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat ttttagatgc
     2821 ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga tttaccagtt
     2881 atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc aagtagatca
     2941 attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata ccaaaaagca
     3001 tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt cgatgttagg
     3061 agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca gtgaattcgt
     3121 caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta atcgtctccc
     3181 tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata agtttttctg
     3241 ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa tagaatctgt
     3301 tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag agatcattgc
     3361 tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg aaaagattga
     3421 ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta atcagcatgt
     3481 taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact ttttgactac
     3541 gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg ggaatgagat
     3601 cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac atgaaatccg
     3661 aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat aaaaaagagg
     3721 ttgtgacaga agggggca
//
LOCUS       DACEMO010000200         3664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEMO010000200 DACEMO010000000
VERSION     DACEMO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.206"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..170)
                     /locus_tag="IW728_002426"
     CDS             complement(<1..170)
                     /locus_tag="IW728_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAQ1025762.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLA"
     gene            complement(534..3200)
                     /locus_tag="IW728_002427"
     CDS             complement(534..3200)
                     /locus_tag="IW728_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1025763.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
BASE COUNT         1009 a          815 c          642 g         1198 t
ORIGIN      
        1 gctaaggaaa gaaatctgcc agatcctcca gcaacgtctg ctgctgtttc agcaggcagg
       61 actttctccg ttttcagata attttttaca tcttcagcaa tagttacttg cgattcttgc
      121 tggatttcat aatattcttc agcaaattca tcccattctt tttcattcat ttgatttcct
      181 ctaatttttt gttatatgtt ttcacttttc ggaataaatc attctcctct tttatctatt
      241 ctatctcttt tgatgaataa taaaatgatt ggctttccgt ctatcataaa taaaaaaagg
      301 gactgtgacc aaagtcgtgt caaagtccct ctttccgaat aaacagtgtc caatcagatg
      361 accctcagta ttagctcaaa atgctgattt tacaacttct tccttaaacg gtgttctcct
      421 gccaactctt cggtcacaag tcacaatatt caacaaacat gagaagctgt ttgttgaaat
      481 tgcaccttgt gactcagaat taaacggcag gctcacaacc tcgttgattc gttttattta
      541 tttccttgta ctcgtacaat gatcaattta gtgatttcca tcaaaacaat tgtcacaagt
      601 gcgattccca tacatgtaaa gacttctgtc aatccaaatg ctgatgggat agagaagact
      661 tctttcagtc ctgggatcat tgttaagcag taaagcgcgc tacatacaac tacagctagt
      721 aagacacttc tgttagagaa gaaccctgct tggatagctg tttgtgtgtt tgaacgtgct
      781 gggaatgttt gcaatgtccg acaccaaatc aatgttgaaa atgacatggc tacaccgaat
      841 tcaggtgaat gctgcatgcc aagccattga gcaaagataa cagcaatccc aatcagcaca
      901 ccacgatacg ttacagaaat caatgttttg tccgtaaaga ttcctgaatt tggatctctt
      961 ggcgggcggt tcatcgtagc aggttcagct ttttctgtac ctaatgcaat tgctggcagt
     1021 gaatcattga ctaaattgat gaacaacagt tgtaatgctg taaatggatt ggaccagtca
     1081 gcaactaagg caaagatgat cgcaatgatc gcacctaaat ttcctgcaaa tagataagaa
     1141 atagctttct tgatattatc aaacacatta cgtcctactt ctactgcgct gacaatagaa
     1201 gcaaagttat catccgtcaa taccatcgct gctgcatctt ttgccacatc tgtacccgtt
     1261 cccatagcaa ttccgatgtc tgcttgtttc aatgctggtg cgtcatttac accatcacct
     1321 gtcatagctg agatatgacc tttattttgc caagcacgaa cgatacgaat tttattctca
     1381 ggtgaaacac gtgcataaac agtgactttt tctaaaactt cgtttagttg atcttctgtc
     1441 aattcatcta gttccgtccc ggtcaaagcc aggtttcctt cttcaaaaat cccgatttgt
     1501 tttgcgatag caacagctgt tgttttgtgg tcaccagtaa tcatgattgt tttgattcct
     1561 gcacttcttg cttctgttac cgcctgagtc acttcagcac gtggaggatc gatcatagca
     1621 agcaaaccaa caaggatcaa atcgtcttca tcttcaaatg ataaatcaaa attatcaagt
     1681 ggacggtaag caaaagctag tacacgtaat gcgcgtgttg aaaaagcttc attttgctct
     1741 tggaattttt tcatcaattc atccgtcatc ggaacgactt caccatttag caatactttt
     1801 ttactgcgtt ggaagacgat atctggtccg cctttaacaa acaagtaatt tttgttatca
     1861 attgtgtgtc cagtagacat taattttcga tctgagtcaa aaggaagttc tgcttgtctt
     1921 gggtagttgt ttcggatttc ctgataaggc tggtttaatt tttcactaaa gtcgataaac
     1981 gctacttccg tagggtcgcc caatttagta ccgtcttcac tgatcgatgc atcattcgct
     2041 aaaacactga tttcgatcaa gcgacgttcg tcagctgtcc attttgaagg gtcatttgtg
     2101 aaatccccag tatcgccatt acctaaaaag tagtcaacaa ctgtcatttt gttttgtgtc
     2161 aatgtaccag tcttatctgt acagatgata cttgtcgaac caagtgtttc aactgctgga
     2221 agcttccgaa taatggcatg acgttttgcc attttattcg ttcccaaaga taacacgatt
     2281 gttacgatag actgtaatgc ttctggaata gcagctacgg ctacggctac ggcaaacatc
     2341 actgcgctga cgatatctgc agtcatatct cctgtacctt ctccaagata aatacgaaac
     2401 agttggatac ctaaaatcaa taaagacaat gccaaaatag ccaaacttag ttttttactg
     2461 aatttatcca gacctcgttg taacggagtg acttgctcag ttgttgattc caaaaggcca
     2521 gcaacttgtc cgatctccgt attattccca gttgaagtca ctaagaattt accgcgtccg
     2581 taagttacga tcgttccact gaataccata tttgtacgat ctccgattgg taccgtactt
     2641 gtgatatccg ttacttcttt ttctgcagaa gtcgactctc ctgtcagcat accttcatct
     2701 acttttaatg atcccgcatc tagtaaacgt ccatcagcag gtacataatc gcctgcgtct
     2761 aacaacacga tatctcctac cacaagttcg tttgccggaa tcgttttttc cttaccatca
     2821 cgaatcactc gagcatcggg cgctgaaagg tttttcaacg cttctaaaga accttctgct
     2881 ttttttgttt ggacaacact gattatagaa ttcaacatca atactgcaaa aataactaag
     2941 aattcgatgt agtcacccat gaccatctga atcagtgcga caatcagcaa tacgataacc
     3001 attgcatctt tgaaagtttc taagaacaat ttccaagttg gcttcttcgg cttttctttt
     3061 agctggttca agccatctct ttctaatttt tccttcgctt gcgtggtgct caatccatcg
     3121 gttgagctct gcgtttgctg catcaacgag tcgatcgttt ctttatagac cgcttgttgt
     3181 gcttgttcct tgttttccaa atttcatccc ccattttttg atcttggccc tagtcctgtc
     3241 tgaaaaaaaa gagacctaag acaaatacgc atttgtctta ggtctcacca tttcatacaa
     3301 taccagaaaa ccatttggtt aacttactgt tgatattatc gcaacaaacg ttgccggtta
     3361 ctcccctaag gagatattca actgtactaa tatcctacct tattaaccta caaaaagcaa
     3421 ctactagccc gatttaccta cattttcatg aaaggagtga aaagtatatt caccttggta
     3481 ggcttctttc ttctatcatg ctatactaat gttattgtat gtaaggagga acatttttgt
     3541 ggtatctagt attataaccg ccctttatct attaaatgca cttattgctt tggtggctat
     3601 tttgatcaaa ccccgagatg tagcagccat ttgggcatgg ctcttagtat tgtttgccct
     3661 tcct
//
LOCUS       DACEMO010000201         3619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEMO010000201 DACEMO010000000
VERSION     DACEMO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.207"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            137..1366
                     /gene="arcA"
                     /locus_tag="IW728_002428"
     CDS             137..1366
                     /gene="arcA"
                     /locus_tag="IW728_002428"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1025764.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            1457..2476
                     /gene="argF"
                     /locus_tag="IW728_002429"
     CDS             1457..2476
                     /gene="argF"
                     /locus_tag="IW728_002429"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1025765.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            2591..3538
                     /gene="arcC"
                     /locus_tag="IW728_002430"
     CDS             2591..3538
                     /gene="arcC"
                     /locus_tag="IW728_002430"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1025766.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
BASE COUNT         1247 a          640 c          769 g          963 t
ORIGIN      
        1 tagaaaaacg tttaattatt taattatcga atattaatgt gaggaatgtc tcattttgaa
       61 tgagacaaaa ttatgaaagt gattacaaaa ggaggatatc ctatacatgt aatcaaaaag
      121 aaggaggcaa cacaacatgg ataaacctat tcacgttttc tctgaaatag ggaaattaaa
      181 gacagtttta ctgaaacggc ctggacaaga agtggaaaat ttgacaccag atattatgga
      241 tcgtctgttg ttcgatgata ttccatatct tccgattgct caagaagaac atgacaactt
      301 tgcgaagaca ttgcaaaatg aaggcgtcga gactctttac ttggaaaaat tagcggcaga
      361 agcgattgac gcaggcaatg tcaaagaaca attcttgaat aaaatgttgg atgaatctca
      421 tattgcatcg aatgctgtaa gagagggtct tcatgagttc cttctatcaa tggaaacgca
      481 agaaatggta gataaaatca tggctggggt tcgtacaaaa gatatcgaag tacgttctag
      541 cagtctatat gatctgtcag cagacgatga ctatccattt tacatggacc caatgccaaa
      601 cttgtacttt acacgcgacc cttccgcatc aatgggaaat gggatgacag tcaataagat
      661 gacttttgaa gcacgtcgtc gtgaatcgat gtttacagaa tatattttga aacatcatcc
      721 aagatttgca aataaaggaa tcgaagtatg gttggatcgt gaaaatccag atcatatcga
      781 aggcggagac gaattgatct taagcgataa agtagtagct gttgggattt ctcaacgaac
      841 taatgcgaaa gctttagaaa aattagcacg ccacctattc gcaaaaaatt caggatttga
      901 aaaagtgttg gcaatcaaaa ttccgaacaa ccgtgcaatg atgcatttgg ataccgtatt
      961 tacaatggta gatcacgata aattcactat ccatccagca attcaaagta aagacggtaa
     1021 gatggatgtc tttacaatcg aaccagatgg tgatgatatc aagattactc attcagataa
     1081 cttacacgaa acgatgaaag ctgctcttgg attagatgat cttgtactga tcccaactgg
     1141 taatggagat gagatcgttg cacctcgaga acaatggaat gatggatcaa acacattggc
     1201 aatcgctcca ggtgttgtag tcacttataa ccgaaattat gtatcgaacg aattgcttcg
     1261 tagctatggc atcaaagtgt tagaaatcaa ttcaagtgaa ttgtcacgag gtcgtggtgg
     1321 tccacgctgc atgagccaac cacttgtccg tgaagacttg aagtaatttg tttcctgacg
     1381 gcagtcttac tgtcagctgc cgtcgggatt tcctctttaa tcttaaataa aaacaatcta
     1441 aaggagaatg tttaatatga aagaatcagt attccaagga agaagcttat tagcagaaaa
     1501 agactttaca aaagaagaat tacaatattt gatcgatttt tcagaacact tgaaagactt
     1561 gaaaaaacgt ggtatcccac atcactatct agaaggaaaa aatattgcgc ttttatttga
     1621 aaaaacatct acacgtactc gttctgcatt tactactgca gcgatcgatc taggtgcaca
     1681 tcctgaatat ctaggtgcaa atgatatcca attaggtaaa aaagaatcca cagaagatac
     1741 agctaaagta ctaggacgta tgtttgacgg aatcgaattc cgtggattca gtcaaaaaat
     1801 ggtagaggaa ctagcagaat tctctggtgt tccagtttgg aatggcttga ctgatgaatg
     1861 gcatccaaca caaatgatcg ctgacttttt aactatccaa gaaaactttg gaacagtaga
     1921 aggtatcact gttgcttact gcggagatgg tcgaaacaat atggctaact ctttattagt
     1981 cacaggtgca attttaggag cgaatatgcg tatcgttgca ccaaaagaat tacaacctga
     2041 agaagaaatc gtcaaaatgg cagaaggctt tgctgaaaaa tccggtgcac aattgatgat
     2101 taccgacgat gttgataaag gtgtagacgg agcagacgtc ctttattctg atgtatgggt
     2161 atcaatgggt gaagaagata aattcgaaga acgtatcaaa ttgttgaaac cttaccaaat
     2221 caatatggaa atggtagaaa aaacacacaa cacagatcgt ttgatcttct tgcactgctt
     2281 gccagctttc catgatacaa acactgttta cggtgaacaa atgaaagaac gctttggcat
     2341 cactgaaatg gaagtaacag atgaagtctt ccgcagcaaa tatgctcgtc aatttgacca
     2401 agcagaaaat cgtatgcact caatcaaagc gatcatggca gcaacattag gtaacttgtt
     2461 tatccctcgc gtttaagcat aaccacattc atctcacgaa aaaacgagtc agcattgagg
     2521 gaaaatcaca aaacgctggc tcgttttgtt atctagaaaa ttcggtagaa ctgaagaaag
     2581 gacgaatgaa atggccaaac gaaaagtagt tgtcgcttta gggggcaatg ccatcttgtc
     2641 tacggatgca agtgcaaaag cccaacaaga agcgttaatg gaaacagcaa agtatttagt
     2701 gaaatttatc gaacaaggag atgaattgat catctcccac ggaaatggtc cgcaagtcgg
     2761 taacctattg attcagcagc aagctgctga ttctgagaaa acaccggcaa tgcctttaga
     2821 tacatgtgtt gcaatgacag aaggatctat cggttactgg ttacaaaacg ccatgggcga
     2881 agtactgaag gaaaaaggga tcgataaaga tgtggtttct ttagtcactc aagttattgt
     2941 ggatgaaaat gatccttcat tcaaaaatcc aagtaaacca gtcggtccat tctacacgga
     3001 agaagaagcc aaagaacaaa tgaatgctga ctccactgtg acattcaaag aagatgctgg
     3061 acgtggatgg agaaaagtag tcgcttcccc taaacccatc tcgattaaag aagcacgtgt
     3121 catcgaaacg ctggtcgatc aaggagttat cacagtctct gttggtggcg gtgggattcc
     3181 tgttgtagaa acagctactg gattagaagg acgagaagcc gtaatcgata aagattttgc
     3241 ttcagaaaaa ttagccgaaa tcatcgatgc ggatttgctg atcgtattga caggagtaga
     3301 caatgtgtat gtcaattacc aaaaaccaga tcaaaagaaa ttagaaactg ttactgtttc
     3361 agaaatgaaa caatacattg atgaaaagca attcgctcca ggaagcatgc ttccgaaagt
     3421 agaagcagct attgcatttg ttgaagcaaa accaaacgct aaggcaatca tcacttcttt
     3481 agaaaatatc gagaatcttt tagcttctga agaaggaacg attatcgtag ctgattaatt
     3541 tgttaagtct ttcgtattta cgaaatgatt tatagctatc caggtggtat ttcttctcct
     3601 ttaggccatc tggattttt
//
LOCUS       DACEMO010000202         3613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEMO010000202 DACEMO010000000
VERSION     DACEMO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.208"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            127..579
                     /locus_tag="IW728_002431"
     CDS             127..579
                     /locus_tag="IW728_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025767.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IW728_002432"
     CDS             593..1282
                     /locus_tag="IW728_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025768.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..>3613
                     /locus_tag="IW728_002433"
     CDS             1407..>3613
                     /locus_tag="IW728_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1025769.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGI"
BASE COUNT         1221 a          480 c          752 g         1160 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aat
//
LOCUS       DACEMO010000203         3601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEMO010000203 DACEMO010000000
VERSION     DACEMO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.209"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..914
                     /locus_tag="IW728_002434"
     CDS             <1..914
                     /locus_tag="IW728_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ1025770.1"
                     /translation="VKICEDYYLEDINISEISKKYGITRYKALKYIEDAREKQLVSIS
                     INSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADYIQELIKKANVVA
                     LSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQKAAERYESSYLTL
                     SIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDSVEPWNKHRKILF
                     PSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGICKSKFKAESCLG
                     ALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            complement(1211..1759)
                     /locus_tag="IW728_002435"
     CDS             complement(1211..1759)
                     /locus_tag="IW728_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025771.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1760..2617)
                     /locus_tag="IW728_002436"
     CDS             complement(1760..2617)
                     /locus_tag="IW728_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1025772.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2903..3190)
                     /locus_tag="IW728_002437"
     CDS             complement(2903..3190)
                     /locus_tag="IW728_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1025773.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYDQLKKLI"
     gene            complement(3180..3509)
                     /locus_tag="IW728_002438"
     CDS             complement(3180..3509)
                     /locus_tag="IW728_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1025774.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT         1086 a          664 c          515 g         1336 t
ORIGIN      
        1 ttgtaaaaat ttgtgaagac tattatctcg aggatatcaa tattagtgaa atttctaaaa
       61 aatatgggat caccagatat aaagctttaa aatatattga agatgcacgt gaaaaacaat
      121 tagttagtat ttcaattaat tctccgtatg ctaggaatta tgagctagaa gaaatcttca
      181 aaaattactt taaaacgaat atctatatat taaaaagtag tgaagactta tcacaacacg
      241 atttattatt ttcaaaattt gctgccgatt acattcaaga attaattaag aaagccaatg
      301 tggttgcact atcttgggga gataccgttt acaaagtgat tgatcagttt aaaatgggtg
      361 ctcgtgaaga attaattttt actcaattca ttggcgaaat cggaaaatat aattccttag
      421 ctggctccac acgattagtt caaaaagctg ctgaacgtta tgagtcttct tatttgactc
      481 tcagtattcc tttatatgtc atcaatgaaa ctgcacgtga actaatggct ctagaacctt
      541 ctttagctaa taccttagca accgcttcgc atgcagatat gttaatttct ggaattggca
      601 ccttatcctc tattgattct gtcgaaccct ggaacaagca tcgtaaaatc ctatttccaa
      661 gcatgacgga tactgttgga tttctttacg gaagaccctt caatatcaat ggtgaattca
      721 tcaaagcatc tgaggataag acttttggtc tttctttaaa agaaattttc gatattccaa
      781 aacgaattgg tatttgtaaa agtaagttca aagcggagag ttgtttaggc gccttgaatg
      841 gtaatttttt tacagattta tttttggatg aaaaaactgc ttggaaaata ctttctttga
      901 taaaaaaaga gtgagtcatg taaaatcctc caaaaaattt agatttttgg tactactttt
      961 ttggagggca gtacaatgat tcactctttt tattggatat caaaatccct ttttttgaga
     1021 agcttgtctt gatatttttt gaacttttcc aagccatact ccattacttt tcaagattta
     1081 atttctttgt attcactgaa taatttccac gttttttcgt taatgttact tcttattaat
     1141 tttattgtac ttctcatgca ataggatcat atatccttct aaatcttttt tatgctataa
     1201 aaagggggtt ctatctaact ttattattca ttccccaccc tttgcgcagt gagtcttcgg
     1261 tatcttgttt tatctgactg gtagttctta ttatttctcc acggtcatct aattctggaa
     1321 caattggata tttttctctg aacaagtcta gttctttcac cacgtcatta aacaaatctt
     1381 gcttatcttc ttgcgtaaga ttctgtttaa tatactcatc taaagctttt ctaagcaatt
     1441 catctcttgt catattagtc tcatactttt ctactaaaac tctggaagtc gcatttaaca
     1501 aagtcgacag ttttaaagga tgcttggtgg aataaacctt atcaaacttt ctgattggtg
     1561 cgccaacttt ttttgttctt cctctactct gtctttcaac attggaggaa gtttttattt
     1621 cttgaacttg gtgcacttct aattgcttat tttcagatgg tgtaattgct tgctgctttt
     1681 tctttgcttc tgcttctttt tttattttgg cgagtccttt gttgttccca aaaatatcaa
     1741 gtcctttgat gtcagccaat tactccacct ccgcatacca attctcgtgt tcttccaatt
     1801 ctttcaaaat actgataaat aattcgtggc ttttgcgatc ccattgtttt aagagacctt
     1861 tctttgtctt ctccatcgta atacctgatc cgtcgtatcg ttttaagcgc gcatttttga
     1921 gtacaacatt tttaatcaaa tgttccccat aaatctcttg ggcttgttta tacatttcct
     1981 gatctactgc gtcaccgctt tccaacatga aaggaataac accagcaaca tataaatttg
     2041 tatcaaattc tttcacaaaa aagtccatat actcaagata ttgcatcgca tttctcatcg
     2101 attttacttg ggtctgtaag acgacgattg cccagtccgc aatgtacatc gcggatttac
     2161 tatatacaga aatagaaggt gggacatcta aatagatgac gtcaaactca tcaaataatg
     2221 gttctagctt ttctttcata tatttgaatt gggataatga atcattatat tggtctttga
     2281 tccaatcatc atacatttca aaggacatat ctgctggaag tatatagagg ttattgatta
     2341 ctttatagac agcctcttta ggtggaagat tgtttatcaa cacgtcagtc acagtatttt
     2401 cgtaaacaat cggttcacct tctccgcctc cttcttcgtt agtgaagttc atatgcgtta
     2461 agttcatcac atcagaagta tttccttgca tatccaagtc aattatcaag gttctttttc
     2521 cttcattaac tgctgattca aaccctaaca tttgcgcgca cgtactttta cctacgccac
     2581 ctttaaaatt accgattaca tatttcttcg ctaccacgtt cctacctctt ttcttttagc
     2641 aatattcatt tgatactatt ataacatatt tatttttagt tactaccaaa aaaaagatat
     2701 atatgtacta ccttataatt aataaggaaa aggtagtaca taaataagga aaagctgaaa
     2761 tatattgatt tatagctttc ctcaaagtaa tgacgttatt taatttatcg agaagcagag
     2821 aatactcgcg actttagtca tgtagacgaa tcagattttc gataaaaaag aagccgtcct
     2881 tcttaagaac gacaacaaat cactagatta attttttcaa ctgatcataa aagttttcat
     2941 gcgttccaca taacaatact ggaataacct tgttttctat aatctcgtaa gctactctat
     3001 atgttgtgcc cgcatatttg aacccactag accaaatacc tgataaatcc ccagtttttt
     3061 gttcaccctt atatggatca atcgcaattt catcataaat taaattcaaa aatttttctt
     3121 tcaatggctt ctcttttagc ttttttaaat acttaatgac atgttttgaa ggaacgattt
     3181 cataagccaa tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta
     3241 tcttcagcaa tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca
     3301 agaatctcat ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa
     3361 tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt
     3421 gcttcaactt cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa
     3481 ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt
     3541 acttagtaaa tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta
     3601 a
//
LOCUS       DACEMO010000204         3570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEMO010000204 DACEMO010000000
VERSION     DACEMO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.210"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            8..1885
                     /gene="tdc"
                     /locus_tag="IW728_002439"
     CDS             8..1885
                     /gene="tdc"
                     /locus_tag="IW728_002439"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1025775.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            2128..3549
                     /locus_tag="IW728_002440"
     CDS             2128..3549
                     /locus_tag="IW728_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1025776.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
BASE COUNT         1130 a          651 c          752 g         1037 t
ORIGIN      
        1 tttcactatg agtgaatcat tgtcgaaaga tctaaaccta aatgccctat tcattgggga
       61 caaagctgag aacggccaaa tctataaagc tttgttgaat gaattggtgg atgaacattt
      121 aggctggcgt caaaactaca tgcctcaaga catgcctatt attacgccag aagaaaaaag
      181 cagtgctagt tttgaacaca ctgtgaataa aacaaaggat gttctatcag aaatttcagc
      241 gcggatgcgt acccattccg ttccttggca taatgccgga cgttactggg gtcatatgaa
      301 ctctgaaaca ttgatgccat ctttactagc gtacaatttt gctatgctat ggaatggtaa
      361 taacgttgcc tatgaatctt ctcctgcaac aagccaaatg gaagaagaag taggaatgga
      421 atttgcaaaa ttaatgagct ataaagatgg ctggggccac atcgttgctg atggttcttt
      481 agcaaactta gaaggacttt ggtatgcccg caacatcaaa tcattgccgc ttgcaatgaa
      541 agaagtgaca cctgaactag ttgctggaaa aagcgattgg gaattaatga acttgtcaac
      601 agaagaaatc atgaatctgt tagacagcgt cccagaaaaa atcgacgaaa tcaaagctca
      661 ctcagcacgt agcggaaaac atttagaaaa attaggaaaa tggttggttc cacaaactaa
      721 acactattca tggctaaaag ctgccgacat catcggtatc ggtttagacc aagtgattcc
      781 agtgccagtt gaccacaact atcgcatgga catcaacgaa cttgaaaaaa tcgttcgtgg
      841 tctagcagct gaaaaaacac caatcctagg tgtagtcggt gttgtaggat caacagaaga
      901 aggagcaatc gacggtatcg ataaaatcgt tgcattgcgt cgcgtattag aaaaagacgg
      961 tatttacttc tacttacacg tagatgctgc ttatggcgga tacggccgtg cgattttctt
     1021 agacgaagac aacaacttca ttccatttga agatttaaaa gatgttcatt acaaatacaa
     1081 tgtctttaca gaaaacaaag attatatctt agaagaagta cacagtgcat ataaagcaat
     1141 cgaagaagca gaatctgtaa cgattgaccc acataaaatg ggctatgttc catattctgc
     1201 cggtggtatc gtcatcaaag atatccgcat gagagacgtt atctcttact ttgcgacata
     1261 tgtatttgaa aaaggcgcag atatcccagc attacttggg gcttatatct tggaaggttc
     1321 aaaagcaggg gcaacagcag ccagcgtttg ggcagcacac catgtcttac ctttgaatgt
     1381 cacaggatat ggtaaattaa tgggtgcatc aatcgaaggt gcacatcgtt tctacaactt
     1441 cttgaatgac ttgtcattta aagtaggaga caaagaaatc gaagttcatc cattaactta
     1501 tccagatttc aatatggtag actatgtatt caaagaaaaa ggcaacgatg acttagttgc
     1561 aatgaataaa ttgaaccatg acgtatatga ttattcttca tacgtgaaag gaagcattta
     1621 cggtaatgag ttcttaactt cacatactga ctttgctatc ccagactatg gcaacagccc
     1681 attacaattt gtgaaccaac taggattctc agatgaagaa tggaaccgtg ccggaaaagt
     1741 aactgtattg cgtgccagcg taatgacacc atacatgaac aaagaagaac actttgaaga
     1801 atatgctgaa aaaatcaaag cagctcttca agaaaaattg gaaaaaatct acgcagacca
     1861 attattggca agcgaagcaa aataagcaag gtaatcatgt tagaaaacag cttgaatact
     1921 aactgtttct gacgcatgtt tggatctgaa gagcaagggg agacctaagc tcttcgatcc
     1981 ctttgttttg aggttgtctt tgaagaaacc cttcaagcaa taaactcaaa gaatcccctc
     2041 aaatattcgc tgaattgttt cgtttattgc tgaaactact tcaaagacac cgttatttta
     2101 tccaaaaaat caaggagagt gtcaacggtg agtaaatctg aagtaagtaa aatgggtctt
     2161 gggaccttta tcggtctgac aatggcactg tgtgcaacgg ttcggagtat tcctacgtta
     2221 gccgcagtcg gttggacatt gattttttat tcaatttttg cagttttgtt ttttgccggt
     2281 ccgatatcaa tggtttccgg tgagctttcc acgatgcttc ctcaagaagg cggtcctcag
     2341 ttatgggtaa aaacagcact gggcagcaaa tggggatttg tcgttgcatg gctattatgg
     2401 gtgcaaatgt tcccaggtat ggtaatggtc gcttctactt taggaccatt gttaggaaac
     2461 acatttggta atgtagaact tggcaataat cactggttcg ttttaggttg tattttagtt
     2521 atctactgga tcatcacgat cttgaacttg aaattcgata tggcaaaagt cggcgggaac
     2581 atcggtgtat ggctaggggt atatatccca gtagtcatca tgttcgtttt aggtgttttg
     2641 gcagccttca aagtcggact agtctcaaac ggctatctag gtgacttttc atggtcaaaa
     2701 gctttccctg acttggaaca tattgattca ttgaaatact tagctggtat cacatttatt
     2761 tttgttggga tcgaaatgag ttctgtttac atgcctcgtt tgaaagacgc aacaaaaaat
     2821 tatacaaaag gcgtatttat cgctttgatc ggattagttt tattgaatgt catcaatgcg
     2881 atgcttgtag caaatgtcgt accagatgga aaaatggaat tagcaaatat cacgcagcct
     2941 atcttgattg actgtcaaat cctaggctta ccagaagtga tcggaaatat cttcagcttc
     3001 atggtattta ttggtgtgct tttacaatta tcagcttggg ttactggacc atcaaaaaca
     3061 atcatccaag tagctcgtga aggattcctt ccaccaaaat tcggattcca taaagaaaac
     3121 aaatacggcg tatctagaaa tgtcgttcta acacaatcaa tcgttatttc attatttgct
     3181 ttgctttatg gtgtaatgga tgacgtaagt gctgtattct tgacacttac aaatgctaca
     3241 actgttattt attgtatcgt gtacattttg attgctgttt cattgcttaa aatgcgtaag
     3301 aaacatccag aattcgaacg tccttaccga attggtaaaa acggaaacgg attagcttgg
     3361 gtagtcagct gcatgctgat cttctctatc atcgttgtcg tatttgctac attaggtaca
     3421 gcgacattga gtgatgcatt attagtagca gctatcacag tggtcatgtt cgttattcca
     3481 ttgatcatca atcacttcaa gaaagattct tgggggattg aggtagaaaa aagtttagaa
     3541 gaaaaataaa aaaataagtc tttcagccga
//
LOCUS       DACEMO010000205         3476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEMO010000205 DACEMO010000000
VERSION     DACEMO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.211"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            76..354
                     /locus_tag="IW728_002441"
                     /pseudo
     CDS             76..354
                     /locus_tag="IW728_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
     gene            497..718
                     /locus_tag="IW728_002442"
     CDS             497..718
                     /locus_tag="IW728_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025777.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            734..1576
                     /locus_tag="IW728_002443"
     CDS             734..1576
                     /locus_tag="IW728_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025778.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1639..3441
                     /locus_tag="IW728_002444"
     CDS             1639..3441
                     /locus_tag="IW728_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ1025779.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
BASE COUNT         1250 a          486 c          710 g         1030 t
ORIGIN      
        1 tcgtcattcc tcctgctatc tttttctatt attctacctt atttgatagt agatttaaaa
       61 ctttgcaaca gaaccaaaga actcatcaag ctggctaata agggtcaaga ttatgaacat
      121 ctagcagatg agattgatga gctgagagac aagcgacagc tccttttagt agaagatgcc
      181 tccctcagtg gcgagaacga gcgaatcaat gagctgattg aatttatccg caagaacaaa
      241 ttccgtacct tagagtacga tgataagctt gtaaggaaga taatccagag cgttacagtc
      301 tatgaagaac acttcgtcat atccttcaaa tctggcatcg aaatggaaat atgaaaacca
      361 gaagtaaata gcccatgatt ctacagtaga gtcgtgggtt ttcttttgct tgctgattat
      421 aataatattt taataactat tgttattata aaccaaatga tttataatat tcttagcgta
      481 aacttggagg tgctgtatga ctacggcaga aatgattaaa gaactgtgtg agcaaatgaa
      541 tataagtgtt tccgaacttg ctagacgtat tggacagact ccacagaatt tcaataagaa
      601 attacaacga gaaacggtaa ccttagatga gttgaaagcg attgctgatg tgctaggtgt
      661 caagtttgtg caggctttta tattgcctga tggcaatgaa ataaagatag gtaattagga
      721 ggtgtaattt gtattgaaaa tcataacaat aaatgatgtt gagtatgctg tatttgctgc
      781 taatgaaggt acttccaaac cacaacctca tatcatagaa acgaaatctg gtactattcc
      841 agaaggaaaa caactgagtt tattaaaaga atatcttaag cagaatgata tttccccaat
      901 aaaaggagca acaacatatt ggtgcattga caaggttctt aagcttgatt caagtaaaga
      961 aaaaactata agtgaaacaa tccataaaca aaaatactta tctttaactg aagaaaatat
     1021 tgagaaacag cataagtttg taggtgcaag ctctaattat gggaaagaag gtctaataat
     1081 acatgatgtt ttgaatgcat ttcctcttca taatgattta aatactattg cgatgaagat
     1141 tgcggtaatt gatgtaacaa attcaacaca tctttctcaa tataagagca ggttgtctct
     1201 atatgatttg gctaaagtaa ttctggagat tccaaatttt gatgatcggt tggcaaaagg
     1261 agatcctcag ttgataaata ttatcgcaag gaacattggt gcagttaata tgttctcatt
     1321 tgcatccaag tactgtacat atcataatgt ggaagtgtgt gggagagatg attactccat
     1381 ctttgatggt atcgttaaaa atactcttcc acactatatc caaggactaa ctacgaataa
     1441 gattgatacg tggcgaagaa gttttgatta cgaggcattt aacgaatgtg ttggaaaact
     1501 acttgatgaa aataatatac atattccttt tagaagaaga aagttagatc acttcttgtg
     1561 gtatgcgaat aggtagaata taagaaaaag gcatcgtaaa agtgattctg gataaaatta
     1621 atgggggagg aaataatagt gggattattt gattttctaa agaaaaaaca aggaaaggtt
     1681 agtaaacacg ttgattatca actggcttct agtctatttc gtactggtaa agtttttgct
     1741 gaaaaattgg aagtggacat atcaaatgta gatgagtttc gtaaaacttt tatcgctttt
     1801 gatgtggaaa caactggcct aaattcttct tcagatagaa ttgtagagat tggtgcggtg
     1861 ctatttaaag aaggagttcc aattcaatca ttcggaactc ttgtcaatcc aaaagttaga
     1921 attgcagcct cagcttcggc aataaataat ataactaatg aaatgctaaa ctctgctcca
     1981 aatgagcagg aagtttaccc ccagttggta gaatttttag gagatgcact agatggaaaa
     2041 atcactatgt gtgcacacaa tgcaagtttc gattttggat ttttaagtaa cacattttct
     2101 agacttgggt atgatgctaa aattaagtat gttgatactc tgagtctctc tagaaaacat
     2161 ataaaaggca ttgtaaatca taagcaagag actgtagcgg aatatttcgg attaataaat
     2221 gacactgccc atagagcgga atcagatgca gatatatgcg gaaagatttt ttggggtgtt
     2281 ttagatatta ttgaggagtc gttggaagaa caaaaaaagc aaatagaaaa gatgactcca
     2341 gacataaaag aacttgaagc ttgtgctttt attcaagata ttattgagaa aaaaggatgc
     2401 gacaccagtt ggatcaggta tcgaaaaaac agtaataatt atgtcgatgt aacttgtcta
     2461 tatacattct taaagtttaa gtttgctaag aagggaaatt acatcattat tagaaaagat
     2521 gctacaaatg gaattgattt acctatagaa ccttgtactg taagtgaagg cggtacaaca
     2581 aatgtgcgag tctattttcg tagccccttt gacttaacgc ctctttcaaa ttatattttc
     2641 agtgcttata gcgattgtta taattctatg aatgaatata tgagtgttag taattacact
     2701 aaaaaagaag cagagcaaag ccttagtatg ctgaaatcta tttcaaaatc agcgatgaaa
     2761 gagttattat tcgatgcaaa aaatcgtgga tatgatgaga ctgaagttaa cctaaatatt
     2821 gaaaaagtga tatctagaga agatgtaatt attaacgggt tgcataatcg agtttcatta
     2881 aacaagatac gaaatatagg gaattgggag aaaggatttg atgcaggtta tccatattgg
     2941 gaacgtggag aaactgctag gaaaaatggc aaagtagatg aagctattgc actttttgat
     3001 aaagctcgat ataacggcta tgatgctcct gcgctatatg attcttatgc taaagcttat
     3061 cgaaaaatta aggattacga taatgaaatt ctcattcttg atgaaggaat tatgcgtaaa
     3121 acacggcacg atgttggtac tttagaggct cgtagggata aggcaattaa gctattattt
     3181 gctaaacaag aggcagagcg gatagctaag gaaaaatctg attttctaaa agcgaacaaa
     3241 aaagaggatg cgattagtga gcctagcaag aagcgtggaa gacagattat tcaaatgact
     3301 gatgatggaa caataataaa agaattcgat actatagctt ctgctgtaaa agaagttggc
     3361 gttaactcaa aaagtattag ggatgctgca aatggagttc aaaaacatgc tggaggatac
     3421 tgctggaaat acaaagatta gcagataatc taagataacc taggatcttc ttaata
//
LOCUS       DACEMO010000206         3471 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEMO010000206 DACEMO010000000
VERSION     DACEMO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.212"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            106..1149
                     /locus_tag="IW728_002445"
     CDS             106..1149
                     /locus_tag="IW728_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1025780.1"
                     /translation="MSEFKYYKSKEVYREKYYQMPKVFFTNEKYLNLSNDAKITYMLL
                     KARFDYSVQNNWVDSEDNIYFIYTIEELMRLLNCREGKISKIKKELEAAGLLKQKKGR
                     IKKANGKIETTPNLLYLGKPEVTSEDVFKINEEERLIPNPVNAKIANTEKDDDIKVIP
                     VNAKIANTEKASDTNSSPVNAKIADNILYSNNSLDTNRHNIDTDKDQLQDQLLLENFV
                     DIMTDSSFDTFIPTNVLKLIKTFSNSYSEAQQTVKTIHNAKHKAQESEKTIIVYEEIE
                     YYGVDADKGLYMTLLKAYQKQKTEKVDNLQNLIFIYVKNWFAEKAIPAKLAHEQNTET
                     NPLPAVNLENWLD"
     gene            2023..2415
                     /locus_tag="IW728_002446"
     CDS             2023..2415
                     /locus_tag="IW728_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgR"
                     /protein_id="HAQ1025781.1"
                     /translation="MELKPKNLKQITCEFVLFNRQHIKFSNRTIKRKITDFSGKEIIL
                     YAKKLSSEQELTCFFDQIKAIYFDGKIVACRGCSELPLIVSNHVFSKMKARQKLLRES
                     KVRYREMQKEEWEKRKSAARKKYKRISA"
BASE COUNT         1329 a          500 c          599 g         1043 t
ORIGIN      
        1 cgtcctgcaa tagtctttgt gtaagtaatt aaaagattta atttattata ttatagcata
       61 ttttaattac ttactccact acatgaaaat aaaaggagta atattatgtc ggaatttaaa
      121 tactacaagt caaaagaagt ttatcgtgaa aaatactatc aaatgcctaa agtatttttt
      181 acaaacgaga aatatttaaa tttatcaaat gatgcgaaaa taacctatat gcttttgaaa
      241 gcacgttttg attactcagt acaaaacaat tgggtagatt ctgaggataa tatttatttt
      301 atttacacca ttgaagagtt aatgagatta ctaaactgtc gagaaggtaa aatttctaaa
      361 ataaaaaaag aattagaggc agcaggactg ctgaagcaaa agaaaggacg tatcaaaaaa
      421 gcaaacggaa aaattgaaac tacgccaaac cttttgtatc taggtaaacc tgaagtaact
      481 tcagaggacg tatttaaaat aaacgaagaa gaacgactaa tcccaaatcc tgtaaatgcg
      541 aaaatcgcaa atacagaaaa agacgatgat atcaaggtta ttcctgtaaa tgcgaaaatc
      601 gcaaatacag aaaaagccag tgacaccaat agttctcctg taaatgcgaa aatcgcagac
      661 aatatattat attctaataa ttctttagac actaatagac acaatataga cactgacaaa
      721 gatcagctac aagatcaatt attgctagag aattttgttg atataatgac tgattccagc
      781 tttgatacct ttataccaac taatgttttg aaattgatta aaactttttc aaatagttac
      841 agtgaagcac aacaaacagt caaaactata cacaatgcaa agcataaagc tcaagaatca
      901 gaaaaaacaa ttattgttta cgaagaaatt gagtattatg gagttgacgc agataaagga
      961 ttgtacatga ctttactgaa ggcatatcaa aaacagaaaa cagaaaaagt tgataaccta
     1021 caaaatctaa tttttattta cgtaaaaaac tggtttgctg agaaagctat tcctgctaaa
     1081 ttggcacatg aacaaaacac agaaactaac ccattacctg ctgtaaatct agaaaattgg
     1141 ttagattaga aaaaaacgaa catatgtttg caatataggc gatgagtggt ataattttat
     1201 tgtgatatat attgcatgat catttaacaa agaaaaaatt agaataaatc atgttatttg
     1261 gtttctaaat tttcattaaa actttcatga gtgttcttta ttcgataacg atagtatctc
     1321 taataaacaa cgtgtaaagt gtgtaatgta agctttgtta tcaatgattt taaatgttaa
     1381 aataattcca gaagcgcttc acaactatgt gagtggaggt ggtgtatatc aaaatataat
     1441 tgttttatgt tgttttacga taattagtac ttacacaaat ttgtcaagtg aaaaataaaa
     1501 aagcttgtaa tgaatttgaa gatgtgctac cgtaaatttg tagaaacgta aattaaaaaa
     1561 attattgatt agcaatccag actgctgatc attgagaagc caagcaataa atcaaccaac
     1621 aaaaaagagc agaccgacta gtgatctcgc tgggtaagag cgccaactct tacttcaggt
     1681 catgaatcgc tagccgtcct caattcaacg tatgtaccac gctgaagagg taaactgcca
     1741 cttttgttgc agatttaact gcaacaggca tattctaaca tatatttctt aagaatttaa
     1801 ctagtttctt tgtgttacct aaattctttt caagaaaatt tgtatttctc tggctacttt
     1861 ttgaatagac ttagtttgca attttctaga tatatttgtt tatactgttt gcagctgatt
     1921 tgtgacttaa gagggcaatt acacttcatg tgtgattgcc ctctttttgt cgtgcttggc
     1981 ttcccaatga tcagcaactg aagcagaagg gcggagaaaa atatggaatt aaaacctaaa
     2041 aatttaaaac aaattacttg cgaatttgta ttgtttaatc gacaacacat aaaattttct
     2101 aatcgaacga tcaagcgtaa aattactgat ttttcaggta aagaaattat tttatacgct
     2161 aaaaaattat caagtgaaca ggaattaact tgcttttttg atcaaataaa agctatctat
     2221 tttgacggca agatcgttgc ttgtagaggc tgttcggagt tacctttaat tgtttcaaat
     2281 catgtgtttt caaaaatgaa agcacgtcag aagctcttaa gagagtcgaa agttcgatat
     2341 agagaaatgc aaaaagagga atgggaaaaa agaaagagtg cggcacgcaa aaaatataaa
     2401 cgaatcagtg cctagctact gttttgacta atgatacttg atttttttgt ttgttagttt
     2461 tttctaacaa acaacctaat agcagggagt tattgctatc gaaaaaacgg acaagtaagc
     2521 acacggacac gaaacagcgc taaatattta tttaacgttt gctggcaagg agtcacatga
     2581 cactaagcga tacacgtaaa cttactttga tgttttgggt gttccttgac aactgaaaaa
     2641 ttacaggact ttctaagtta atcagcgtgt ctaatagggg cttaggaagg ataaaccacc
     2701 taaaaaagta cggctgtgtt atcagatggt acgttattga tgggtagggc acacacagaa
     2761 aaggttattt gcgatgatca gctatcacga aaaacataag atgacgatta aaaccgtccg
     2821 ctcgtttttc tagggagcaa aaacactttt cgagagtttg gggttgctat cgctagaaac
     2881 cctgcgtaaa aaaactgtaa catttatgag aaaactaata aacaaaagaa tcaagtatct
     2941 aaaaataagg ggacacctaa tttaactgga gggtacacct atgagtactg agaaaaaaga
     3001 aaaaagtatg cattttctga tgaaactata gaaaaattgg attggctagt aaaagagcac
     3061 caaaaaagaa gtaaaaaacg tatttatcct tgtgatacac tttctgaatt gatcaataat
     3121 gaatatgtga taagaaagag ttttggcaat tcataagtaa tagtttactt ctttaattcc
     3181 ttaacaagta tataaaggag ctgagaaatt tgaatcgtat tcgatttggt aaaagtaata
     3241 aaaacgagtt ctcatgacga agtgtatttt gaatatgatt tagccactca tagaaattat
     3301 aatcctaaag aatctcatga agtatcttta agaagagttc aatatctgac ttgggattta
     3361 tatccacttt taaaatatcc agaaagtgta actagcaaac ttttttatat tgataaaagg
     3421 aataacgaaa aagtagagaa agcgtgggtt actcttctaa aactgcaaaa a
//
LOCUS       DACEMO010000207         3469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEMO010000207 DACEMO010000000
VERSION     DACEMO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.213"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            71..3349
                     /locus_tag="IW728_002447"
     CDS             71..3349
                     /locus_tag="IW728_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1025782.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         1216 a          627 c          769 g          857 t
ORIGIN      
        1 attgacagta aaaataaatt aggtaatact ttaagtaaac gctttctaat ttgttacggg
       61 aggaatacac ttgttaaaaa aacgtataaa atacagtgcg ctaatgatga atctccttat
      121 gctcagtacc ccggttttaa tttctgtcgt tgaagttgct gctaacgaac aacagtctgt
      181 gaacgaagaa aatgcacacg tgaacattga agggatgtca acaatggagg aaataccaag
      241 aactagtgaa gatatggata cggaagaagg aaatgatcct atgatgaaag aatcagaaaa
      301 tgaaaagaca aaaaagcaat ctgaagaagc aaatgaagag tggaaagggg ttgttttcgg
      361 agaaagcaca agtgctagta ggtatgccat tgaaccatta gaagacggcg tgcggctctc
      421 ttccacgaat aacggcggaa aattccagtc aagcggatca gatggaatga cctattatta
      481 tcaagcaatc cccaaagaca tcaactttag gatgcgggcg acgatcgaaa tagaatcttg
      541 gacctatacg aatgcccaag aaggatttgc tttaatggta agagacgcag taccaaaaaa
      601 tcatttgttt gggcaggcat tttattcaaa ttcatttgcc atactaggca gtcggatcga
      661 atatgtctgg gactcggata gacaagaagt ggtcaacacg agcggcagca aatatacgat
      721 gcgacttgga ttggggacac gggcaatcac aggaatatca tctgaagatc cgcaagctgc
      781 gccagctcct gggagtgtct cagtggaaac aacaccttta gagacttcag cagggttctt
      841 ggaaaaagaa acaggttctt ataatatatt cggaaacggt catgggaatc tgtttccggc
      901 cacgaactcg attactaaac tggacgtaga gatgaaaaaa acgaatactg gtttagaggc
      961 atattattac gatcctgaaa caggagaaga aatggcttct gacatcatgt atgactggga
     1021 aaaattatac gcttcagatg aatcggttat ttatgcagga tttgctgcag caaggaatat
     1081 gacgatcaag gtagaaggaa tcgaagttgc ttactcagat cctgcgacag atcctcctgg
     1141 gcaagaaaga cctactcgct tgattgatcc gatttacact gtgacctcta gtaatcattc
     1201 aggaaaccaa gatcatacta tgtcctttag agcaaatgct gacggattat tgacaattaa
     1261 aaataaagca acaggagagg tgcttaagaa cgcaaaaaac ttggctatca ctgcgaatcg
     1321 ggaatttgat tatcagacaa aactgatgga aggaacgaac gaatttactt tcagttttac
     1381 gccagataaa aactatccgc cagaagaata cgtagagatg actagttatg aaacaaaaga
     1441 gattcttcat acagtggact accgtaaacc taaatattcg acagtttatg taacaccaga
     1501 agggtcagat gcaggaaacg gaagcagaca aaatccatta tctctgacac aagcattgag
     1561 atacgcatat gctgggcaga caatcgtcat ggcgcctgga acttattcct tcgaaagagg
     1621 attgacgatt ccaaaaggcg ttaacgggac aagagaaaac ccgattcagt tgattgcaga
     1681 gaaaaatcca gaaaacaagc gtcctgtact tgattttaaa ggaactggaa atgggatgac
     1741 actgttcagt cactactggg gtttacgagg gtttgacatt acaaatagtg cagctatgca
     1801 aaaaggatta cagatttctg gaaatcataa tttgatcgaa gagattcatg cttatcggaa
     1861 tggaaataca ggaatccaaa tcagcggtag ctcaaatgat cctttcgaag catggcccgc
     1921 gcataactta gtcaagaact gtacatcttt tgaaaatgcc gatccaggat ttgaagatgc
     1981 cgatggattt gcggccaaac tgacaatagg agaaggaaat gtatttgacg gttgtatcgc
     2041 gtatcataat gcagatgacg gttgggacct ttttgctaaa aatgaaacag gttcgatcgg
     2101 aaaagtaatc atcaaaaact cagttgctta tcgaaacggc tgggtaccag gaattgaggg
     2161 agaaggtaac ggtaatggat tcaaaatggg cggttcttct ttaacgggcc ctcatgaatt
     2221 gatcaatagt ttgtctttcg aaaatcttgc gaaaggaatc gattccaata gcggaacaga
     2281 tattatcgtc aatagcagta caagttttaa taacggttca tataatgtgg ctctttatac
     2341 ctcttcagca ggaaacacag actaccatgc ttctggtatc ttatctttcc gaacagagaa
     2401 tcttgagatg agagagcaga tccgcccatt gaatcaaaac gaagatcaaa tctaccactc
     2461 gttgaactac tattggaatg aacgagagaa acaatcagaa aatactcttg gacataccat
     2521 atcaaaagat tggatcgagt ctctaaatac atgttcgaaa gaaggtcagc agcctgttac
     2581 gcgctatgaa aatggaagta tccatgttca tggactgatg cagattaaac cggggaatcg
     2641 tcaaacagaa aaggaaagag tggatggtct tccattatct gtaggtgcta tttttgacgg
     2701 agaaacctca ccgtcttcgg aatatccaga gtatgcaatc gtacaagaac caaatcagac
     2761 aaaaccaaat gaacaaaata atccgattat tatacaagaa gcaaatggtg tggcggagaa
     2821 tgcagaagga aatatcattg aactgaagcc ttttacagtt tttgtgtcaa ctttcacacc
     2881 aaatcaagga acgcctgtct ttgaagatat gcgttatttc ccagaaaaga tccaaatcgt
     2941 tgaaacacag tcaagtgctc aattttctaa tcataccgct attttttcag caccttcaaa
     3001 aggagaatat acgctagaag tcacttatcg ttcggaaata tttgatgggg aaaaatggtc
     3061 tgctcataca gaatctgtta cccagcaaaa agcgatcact gtgcgagaaa ctgccgatgt
     3121 gcccccggga aatgacaaac cagaaagaga aaatccagga aatgaatcac caaatggaaa
     3181 accaaatgga aaaccttctc atccggatag gacagcaaaa gaaagtgaaa aaaatggcac
     3241 tcaaggactc ccaaaaacgg gagaatttaa taacccgttg ctagcattag ctggaggaat
     3301 tttgttaatc ggtgtagttg tttatgtcat gaaacagcga aaaaaataag tggaatttct
     3361 ccttttatga tgtaaaacaa ttgactgagg aaagtaaaaa tagaaggaaa tgagaccaat
     3421 agatgtctca cttccttcta tctttttata gatgaggttc tccttgctg
//
LOCUS       DACEMO010000208         3420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEMO010000208 DACEMO010000000
VERSION     DACEMO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.214"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            38..1777
                     /gene="yfcC"
                     /locus_tag="IW728_002448"
     CDS             38..1777
                     /gene="yfcC"
                     /locus_tag="IW728_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1025783.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1934..3118
                     /gene="iadA"
                     /locus_tag="IW728_002449"
     CDS             1934..3118
                     /gene="iadA"
                     /locus_tag="IW728_002449"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1025784.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3229..>3420
                     /locus_tag="IW728_002450"
     CDS             3229..>3420
                     /locus_tag="IW728_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1025785.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKD"
BASE COUNT         1075 a          578 c          811 g          956 t
ORIGIN      
        1 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
       61 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      121 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      181 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      241 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      301 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      361 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      421 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      481 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      541 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      601 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      661 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      721 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      781 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      841 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
      901 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
      961 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1021 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1081 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1141 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1201 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1261 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1321 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1381 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1441 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1501 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1561 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1621 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1681 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1741 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1801 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1861 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     1921 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     1981 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2041 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2101 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2161 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2221 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2281 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2341 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2401 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2461 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2521 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2581 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2641 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2701 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2761 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2821 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     2881 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     2941 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3001 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3061 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3121 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3181 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3241 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3301 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3361 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
//
LOCUS       DACEMO010000209         3376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEMO010000209 DACEMO010000000
VERSION     DACEMO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.215"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1086
                     /locus_tag="IW728_002451"
     CDS             <1..1086
                     /locus_tag="IW728_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1025786.1"
                     /translation="LSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGV
                     YLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLP
                     PLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWII
                     FGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSS
                     YVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGML
                     VAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGIL
                     VPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1052..1402
                     /locus_tag="IW728_002452"
     CDS             1052..1402
                     /locus_tag="IW728_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025787.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1494..1913)
                     /locus_tag="IW728_002453"
                     /pseudo
     CDS             complement(1494..1913)
                     /locus_tag="IW728_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1914..2258)
                     /locus_tag="IW728_002454"
     CDS             complement(1914..2258)
                     /locus_tag="IW728_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1025788.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2524..>3376
                     /locus_tag="IW728_002455"
     CDS             2524..>3376
                     /locus_tag="IW728_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1025789.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQS"
BASE COUNT          938 a          646 c          616 g         1176 t
ORIGIN      
        1 ctttctgtgc ttttagctta tcggcacaag gtgattttaa acggtccctt atttgcttcg
       61 attctaacag ttaccggttt ctcttttttt ggcaaaaacc tttataattc tatctcaatc
      121 attttaggtg tgtatctata cgctgtattt gtcaataaac ctttttctca gtacatcatg
      181 atcggactgt ttggtagcgc attatcaccg gtcgttagtt atatcacttt tggtatgcgt
      241 tttcctttac tgataggtat tttattgggg aatttagctg gcatagcaat tggtctactt
      301 cttccgccct tagctgctca aactttagtt ttccatcgtg gattcacact ttataatatt
      361 ggattcacct ctgggttgat tgcgatgact tttactgctg ttttgaggtt attcagctat
      421 tcaatcgtcg aaaatacatt ggttttcaat gaatatcact ttcctctcat ttggattatt
      481 tttggctttt tttcgctgac agtcggaatt ggtttctatt ataattcctt tcggcttttg
      541 gggattcgag aaattttcga ttcatctgga aaattgacaa ctgatttcat tgctaattct
      601 ggtattggtg ctactttgat caacataggc ttagttggtc tgatgctgtc ttcttatgtt
      661 ttgctggttg gcggtcagtt aaatggacct gtcatcggtg ctattttatc tgcggtgggt
      721 ttcagtgctt ttggttgtca tttaaaaaac agttttccga tactagtcgg aatatttatt
      781 gcttctttgt ttggaacttt tcatgagatc accagtactg gcatgcttgt tgcagctgtg
      841 tttgggacag gtctcgcgcc cataagcggt ttttacggca gtttttacgg tgtaatagct
      901 ggtgtactac atattgctct tgtccataac gtcagcactt tgcacggagg actaaatcta
      961 tataacagtg gtttttcaac tggtttcgtt gcaggtatcc ttgtaccgat actagataac
     1021 tttaccgctg tcagaaagga aaaaaagaca cttggaaaaa gaattatcaa aaagaatcat
     1081 cgatgagctt cttacttatt tatattccca tgaaataact gaaatacgta ttggagtaga
     1141 atttgctgag gaagggcttt tcatagagat acagggtacg gcagaaactc agccagaaga
     1201 tttagatgta cttgctgatt tgctgaacaa tcctcgggac cctagtatgg aaatctatta
     1261 tcaagaacta ttaggtaatc cccgacatga tcgggaagat tatcatctgt taggtagttt
     1321 gattgatgac gtggaaatca tgtatgccca gccgattatc aatattaaga tctttcgacg
     1381 tgcttctcat tcctattatt aaccctattt tctatttata aaagactgaa tcaaaaaagc
     1441 agctcctgaa agttcgatta aaatctactt tcaggagctg ttcaagcttg acattattga
     1501 ttttccaatc cttcatacac ttcatctgtt acaggttcta aaaactctga attaccagca
     1561 gtgatcgcca aatgttcaaa ccaactgtct tttgtcgcac catgccaatg tttaatccct
     1621 ttgtgcgtta cgacgacatc tccagccatt aatcgtcttg ctggctttcc ttcttcctga
     1681 taccagcctt ctcctccggt cacaagtaag atttgatagc cgtctttatg gacatgccag
     1741 tgattgcgac aacctggttc aaaagttaca ttacctacac caacagaaac ttcgggatca
     1801 ttcactaaca tattcagata gctttacccg gtaaatactt gagcgtaagc ttcgtttttt
     1861 tcacctgctg gaaaaatcac gccattgtgt atttcttcgt gttttgtcgt catttacgcc
     1921 tctttcttat cttcgctatt ttcttctaaa tagatttctt ttgctaaact aaaagctgac
     1981 caagcttttg gccatcctac ataaaaagct aaatgggtaa tgatttctgc tatttcttca
     2041 ctagtaatgc cattttgctt gccaatcttc agatgagctg gcaattgttc aaaatttcct
     2101 cctgaaatta atgcagatat ggtgatgata ctacgctgat gagcagataa ttcttctctg
     2161 gaccatactt gtccaaataa aacatcatca ttcaattctg caaattttgg tgcaaaatct
     2221 cctaattgat ctcgtcctgc ggtttgtttt tctgccattt ctcttattct cccttcacga
     2281 ctgttaggtg attcaactat ggttctaagg caactttatt ttctattgtt tcttcgcatt
     2341 tcattttaaa tgtagtcttt cagcctgtcc aatgcttagt ttattcagtc gatatgcttt
     2401 ttaagcatag ttgctaacgc tttttttatt cgccgattgt aaaattaagc tagacaacta
     2461 aaaaagtcat ttgtgctaca ctcaaaatag ttccgaccaa agaattataa ggagtgagta
     2521 caaatgacct atacccatct tacaacggat gaacttgtaa taatagagtc ttatttcaaa
     2581 atgaatcaat ctgttgctaa aactgcccat tgcttgaatc gttcaagaca aacgatccat
     2641 aaagtatatc tgttcttcaa gcaaggaaaa tcagccttag aatattatca acagtataag
     2701 aaaaacaaat caaactgtgg tagacgtccg cttgttttac ccgaggaaca atcagaatat
     2761 attcaaagaa aggttgttca aggatggaca cccgatgtga ttgttggtcg tgcagcgttt
     2821 cctattagtt gttctgctcg taccatttat cgtatgttca aaaaggggct atttgattct
     2881 tctgacttac cgatgaaagg caagcgtaaa ccgaatggac atcaagaaag acgtggaaaa
     2941 caaactttcc gccgctctat tcatgaacgt gaaaaggatt atagccaatt ctcaaatgag
     3001 tttggtcacc ttgaaggtga cactatcgta ggtctgaaac ataaaagtgc tgtaattacc
     3061 ttagttgaac gattatcaaa agttatcatc acattgaaac cgtgtggtag acaagcgatt
     3121 gatattgaaa aaaaattaaa tcaatggttt gaatctgtac cgaaaaacct attcaaatcc
     3181 atcacttttg attgtggaaa ggaattttca aattggaaac agatcagtaa tgtcaatgat
     3241 attgccattt atttcgctga tccaggaacg ccgtctcaaa gaggcctaaa cgagaattct
     3301 aacggattgt tacgtagaga tggtttattg aaatctatgg atttcaattc agtagatgaa
     3361 ttttttattc aatctg
//
LOCUS       DACEMO010000210         3375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEMO010000210 DACEMO010000000
VERSION     DACEMO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.216"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(254..757)
                     /locus_tag="IW728_002456"
     CDS             complement(254..757)
                     /locus_tag="IW728_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1025790.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1116..2141
                     /locus_tag="IW728_002457"
     CDS             1116..2141
                     /locus_tag="IW728_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1025791.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2464..3342
                     /locus_tag="IW728_002458"
     CDS             2464..3342
                     /locus_tag="IW728_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025792.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         1079 a          534 c          643 g         1119 t
ORIGIN      
        1 gacggaattc ctttctagat tgagatattg actaggaaga atgttaagat tttaagcctt
       61 taagctaaaa aaaaaccctt tttgttaaat aaggggaata tagcctgtca gtttcaaact
      121 gcaagtcgtc ctgctgcaat aatatagtag attagagatg aaaatagatt tttttaaacc
      181 aatgactgaa taaaaagacc gtcatagata cacattggaa tgtgtctatt acggtctttt
      241 tttacgaacg attttattga agacggacag ttcgggtcaa aaataaaaaa gctacgatca
      301 gaaataagaa taaaatgtat cccaaaataa tggctagacg gtctaactta tttgtcgata
      361 gatgtttgtt tagaagttga taggcccata aacctatcag tcctcctgtt gtattagtta
      421 tcaaatcagt gatgtccgct acgccaatac cgaataggaa ttgaatcaat tctgccaaga
      481 agcttacaag gaaaataatc cctaattttt tagaaaaaga aagtctcttg aaattgacat
      541 tcaaaagcac cccaaaagga aaaaagacaa tcaagttata agtggtctca cgtaataccg
      601 tcgtattttc aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg taagaataag
      661 tgagaaattg tggaagatta aatgatagct tgaataaaat caaccataat agaaagaata
      721 aataaatccc taataaaaat tttgatacat accgcataga catctcctcc atttaaaaag
      781 tataccatcg aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg accaacgtag
      841 ttatcgtacg aaaattagaa gctttttttt gttgggaatc cggtttcttt aacaaagcta
      901 agatagcgag ttgcttggaa aagttgctat cttttttctg tcaaaacgac tcggaatgtt
      961 cgttttttgt tctaatttct ccaagttgtt tactttttga tactaagttt atgaaaaatc
     1021 aagaaatagt tgttatacta ggtaatgctg acaaacggga ttggcatttc atcttggttc
     1081 agcttttttt ccaattacta ggaaggaggg atagattgtt caagacttat tttcgtttca
     1141 ataaaataca agataatcct tttcgtatca tgaatctgat gctacttatc tttattgtat
     1201 tatggttagg ttacataaat catgattttt tgatttcaag tttttcatca ttagggatct
     1261 ttactttttt ctattatcaa aacataccga tgaaatattt gctgaaacgt atgtttctga
     1321 tcggttgcgg actgttggtt gctttcgttt tggggatctt atcgacttat gttttatggc
     1381 ttgaaccatt tgccgtagct ttggttgctt tttccagcag attcgtgttg cgattattcc
     1441 atatttctaa accaggtggc ttgttttttg cgatgctttc tgccatggga acgagcatgc
     1501 agctgccaat cgctcaatta ccgatcgtct cactttattt ttttatgggt gtcgtttttg
     1561 ccctgattgc ggcagtgatt acaaaattac tggattcacg gcctgagcag acaattgaaa
     1621 aagcaacttt aaaagaaaga tttcacgaag agcctttagt gatcattgat tctgttttct
     1681 acagcgctgc cttatttctt tccgtttatg tcagtcacgg cttgaatctg cataatcctt
     1741 attggttgac cttgtcatgt gcatcgattt tgctagcaga aaacttggat gcgatgaagc
     1801 atcgacaagt ccaatatctg attggatcga tgggtgggtt atgtgtgtcg gcattcttgt
     1861 catttgtgcc gtttacgcag ttgcagacca tttttctgat cacttttcta tatggaatcg
     1921 cacaatttct agttgctaga aattatgcag ttgctaatat ttttcttaat ccgatggcgt
     1981 tgatgctatc tacacttatt cgtggagcat acttgatttc tttgatcgaa tatcgttttt
     2041 tagggatcgt cataggaagt ttcatcggtt taggtgttgc gtgggtaatg acggttggtt
     2101 tgcaacatta tttaacggtt gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc
     2161 aaaagaggtt atgacaagat ttttgtcaca acctctaatt caaggtgttc aaaagccaga
     2221 ttctcagcat taagtcaaat tcacacaaaa atgtggaaag gaactttcaa aaaaatttta
     2281 tatttattct atcaaaaaat tttgtttatg atggtaactg aataaacttt cctgagatgg
     2341 tcaatgcttt ccttgaaagt cttacagata ttctttcttc atagaaagtt taagttttat
     2401 agttagtgta caataaaagt atgtttatac catatggtgt ccggataatc agtcatttaa
     2461 acaatgaggt tcaaagatat gaaaagggga aatttgatga agaaaataag caatgaaatc
     2521 caaattctgg cagaaacttt aactcttccc aaagtcgtta tagagcaaat gattgtacaa
     2581 gcaaaaaaca tccttgatga ggtgtttgag caatatagtc atgaactaat ggatcctaat
     2641 aaagcaaagg cgactgcaaa gaaaatagac aaagtctatg aagaggaacc ttttgcagca
     2701 ttagctattt atctatatgc agcttctcaa agttggaaaa aaatttatca accattgaag
     2761 attccaagaa ctatctattt ggcaacaatg aatgctttta ctagatttat tcatgaacat
     2821 ttccaagtaa caggaagcta tcgctttgac cgtggttttt ggatttggca ttacactagt
     2881 ggcttgatct ttcggattgg tgaattggag tacgaaatga catattttcc gaaagggtac
     2941 acggatctaa gattagaagg aaagcagtgc ctttctcttc atattccttc cgatgcagat
     3001 ttatctcaga ataagataag agatagttac gaaagtgcca ttcgtttttt tgaactttat
     3061 tttcctgatt accattacca agtgatgtac acagatactt ggttactatc acccaatctt
     3121 actaaatggt tgaaaaaaga gtcaaaaatc tgtctttttg cagccgatta tcgattatta
     3181 tcagttgatg aacaggacga tagtggagtc ccttggatct ttggacgagt agatgcccaa
     3241 atacatgatt atccagaaag tacgagttta cagcgacaag caaaagagca attgcttgca
     3301 ggcgagcaca ttggctctgg cttaggtatt cttacttcat aagtttagta cttcgaaata
     3361 gtttaaaaga gcgtg
//
LOCUS       DACEMO010000211         3340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEMO010000211 DACEMO010000000
VERSION     DACEMO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.217"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..442
                     /locus_tag="IW728_002459"
     CDS             <1..442
                     /locus_tag="IW728_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1025793.1"
                     /translation="LEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATN
                     AVLGSNAIEQLKNFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLID
                     HTKFNQLSFTSVAPLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            439..1353
                     /gene="pfkB"
                     /locus_tag="IW728_002460"
     CDS             439..1353
                     /gene="pfkB"
                     /locus_tag="IW728_002460"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1025794.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1371..3302
                     /locus_tag="IW728_002461"
     CDS             1371..3302
                     /locus_tag="IW728_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1025795.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1052 a          611 c          771 g          906 t
ORIGIN      
        1 gttagaaatg attccttttc tgcaaggaaa acagatcaca gtagtgacaa attccgttca
       61 acatgcggca aatttagttg atttggagct tgctaccatt attttaggtg gaacgataaa
      121 gctggcaact aatgctgttt taggatctaa tgcgatagaa caactaaaaa attttcattt
      181 taataaagca tttatgggaa tgaacggtgc ccatcttgaa caaggattca cgactccgga
      241 tccagaagag gcagctgtta aacgtttagc catgaaaaat tccgaagaga catttgtatt
      301 gattgatcat accaaattca accagttgtc cttcacctct gttgcacctt tggaagcagc
      361 cacgatcata accgagcgtt gtccattaga attcgttaat gaattccgtg aaaagacaac
      421 aattaaggag gcaatacaat gatttacaca gtaacactta atccatcaat tgattatatc
      481 gtacgcgtag atgggttaaa actaggcgat ttgaaccgaa tgaaggaaga cttcaagtta
      541 cctggaggaa aagggatcaa tgtttcccgt attttgaaac ggatcgattc tcaatctaca
      601 gcactagggt ttttaggagg gttcactggc gaatttattt cgaagtggct tgaaaaagaa
      661 caaatccagt ctttgttcac aaccgtaaaa gaagatacaa gaatcaatat caaactaaaa
      721 tcagacactg aaactgagat caatggctta ggcccatcta tttcagatga agaaatcgat
      781 gagttgaagc aagcattagg aacagtagga caaggagata tcgtcgtatt gtcaggaagc
      841 gcgccagcta gcctgcgaaa aggattttat caagaactga ttgatatcat tcgagaaaaa
      901 ggtgcagatt tcgtgattga tacaacaggt gatgacctaa aaaatgcact tgttaagaaa
      961 ccgttgttga tcaaaccaaa caatcacgaa ttagctgagc tttacaacac aaccttccaa
     1021 tctgtagaag aaatcattcc gtatgggaaa aaattactag ctgaaggggc acagaatgtg
     1081 ctgatctcaa tggctggaga cggtgctttg ctcttcacgg aacaaggaac ttataaaacg
     1141 aatgtattag ttcgcccatt gaaaaattca gtaggtgcag gggattcaat gattgctggc
     1201 tttattgggg cttacagccg tacgaaagat ccaatcgaag cctttaaatg gggagttgct
     1261 tgcggaagcg caacagcttt ttctgatgac ttggcagctc gcgaattgat tgatgagttg
     1321 ctgccagaag ttgaaatcac aaaaattgat tagtttcaag gaggataatc atgaatatca
     1381 aggatttaat ggtcaaagaa gcgatgatca tggatttgca agcgactgat aaaaaaggcg
     1441 caatcgatga aatggtccaa aaaatgtttg atgccggccg gatttccgat atcgaaacat
     1501 ataaagaagg aattctagcc cgcgaagcac aaacttctac aggtcttggt gatggaatcg
     1561 ccatgcctca cgcgaaaaac agtgcagtta aagaagcaac agttttattc gcaaaaagcc
     1621 aaaaaggagt agattatgaa gccttagacg gacagccaac ttatctgttt ttcatgatcg
     1681 cagctccaga aggcgcaaac gacacacatt tgcaagcatt agcagcacta tctcgattgc
     1741 tgattgatgc agatttcgta ggaaaattaa aagaagcaaa tacaccagaa gaagtccaag
     1801 acttgttcca aacagctgaa gagcaaaaag aagcagaaga aaaagcagaa caagaaacac
     1861 aagctgcaga agcttctgct catccagaaa gaaaattcgt tgttgccgtt acagcttgtc
     1921 caacagggat cgctcacact tacatggcag aagatgcttt gaagaaaaaa gcgaaagaaa
     1981 tgggtgtgga gatcaaagtc gaaacgaacg gttcagaagg aatcaaaaac cgattgactg
     2041 ctgaagacat cgcccgtgca gaaggtgtaa tcgttgcagc agacaaaaaa gtagagatga
     2101 atcgctttga cggaaaagaa ctgatcaatc gtcctgtcag tgatggtatc cgtaaaccag
     2161 aagagttgat caatctagcc cttagtggta ctgcaccagt attccatggt gatggaaaag
     2221 aaagcagttc agaagaagga aatgcggatg gtactatcgg acaaagaatt tacaaagatt
     2281 tgatgaacgg cgtatcacac atgttacctt tcgttattgg tggaggaatc gctattgcgc
     2341 tttctttcat gatcgaccaa tttattggcg tgccacatga tcaattatca aatctaggga
     2401 attacaatca agctgctagc tggttcaacc aaatcggaca agctgcattt ggtttcatgc
     2461 tgccggtatt agcaggattt attgcttcaa gtatcggtga ccgccctggt ttgatcgttg
     2521 gatttgctgc tggtgcatta gctaatacag gcggtgcagg attcttaggt gcactgattg
     2581 gtggattctt agctggttat gtcatcgtat ttttacgtaa attattcaag aacttaccaa
     2641 aatcattgga tggaattaaa acgatcctct tttatccagt gtttggctta ctgattactg
     2701 gtttcttgat gttgctggtc aatgtcccta tgaaagcaat caatgatgca ttgaacggtt
     2761 tcttgacaag tatgagtggt tcgaatgctg ctttacttgg tgcattatta gctggaatga
     2821 tggcggcaga tttaggcggt ccaatcaaca aagccgctta tgtgtttggt acagcaactt
     2881 tagctacgac tgttgcaact ggcggaagtg tcgtgatggc ttctgttatg gctggaggaa
     2941 tggtacctcc attggcaatt ttcgttgcta cacgtttgtt taagaataaa ttcagcaaaa
     3001 cagatcaaga tgctggtttg acgaacatcg tgatgggact atcgtttgtc acggaaggat
     3061 cgattccatt tgcagcagct gacccgattc gtgccattcc aagttttatt attggttctg
     3121 ctttaactgg cggattagtc ggagctttcg gaatcaaatt actggctcct cacggcggga
     3181 ttttcgttgt attcttactg agtcacccgg tcatgtattt ggtgtttatt gctatcggag
     3241 cagtcgtatc tggtgtgatt tatggtttat tgagaaaagc tcctgcagta gcagaagcat
     3301 agaaataaaa aagaagaggc tgggttttcc cagcctcttc
//
LOCUS       DACEMO010000212         3338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEMO010000212 DACEMO010000000
VERSION     DACEMO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.218"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(154..639)
                     /locus_tag="IW728_002462"
     CDS             complement(154..639)
                     /locus_tag="IW728_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1025796.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1119..1760
                     /locus_tag="IW728_002463"
     CDS             1119..1760
                     /locus_tag="IW728_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1025797.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1779..2414
                     /locus_tag="IW728_002464"
     CDS             1779..2414
                     /locus_tag="IW728_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025798.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2432..3247
                     /locus_tag="IW728_002465"
     CDS             2432..3247
                     /locus_tag="IW728_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1025799.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1084 a          550 c          721 g          983 t
ORIGIN      
        1 ggatttggct aactatggaa gaatactcgt attagttttc agcgttcata ctagttgtta
       61 cattgtttga ttggccaaaa gaatttctga tatattttgt gctaaataaa gaaaaagacg
      121 cttgaattgg gaaataagaa aactactcta tcattacaaa ttttcataaa ctggcagatg
      181 ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt
      241 gatatttgga tgaaagccta cggtatcttg acgatcaata gcagcatcga tcacgacttg
      301 gatttttttc tctttgtcgt gcatcaatgc taatggagtc actgtacctg ctggaacgcc
      361 taggaggtca ggaagctgtt cttcagaaac aaaagataag cgcttttctt ttaattgttc
      421 agctagttgt tttaaatcgg cgtgcttttc atcaggcaag atgacaagat agatttgttt
      481 tccttttctt gatttcaata atagattttt tacttgcgga ccaggtaggg aaaaagggac
      541 atttttgact gacgtaatcg caggatgatc gactttttga aaagagatac ccagtttttt
      601 tagtagttca taagcttctt gttcggttgc acgactcatc gcgcatcacc ctcctttttt
      661 tattagtgta tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa
      721 agttaaaaca aatgacatcg aaaagaagag tagccgttag gtgtttttag agagtctctg
      781 gttggtggaa agaggcagca gttaaccgtg aaacacatct tggagtcgat tttctgaagc
      841 gtatagtagg aaaatccggg gagcccgtta cagctgcggt cgttgctaga tgaccagata
      901 aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat taacgtcctt
      961 tgatcttcgg atcaaaggac tttttgtttt catctgaacg gcaacgactg tgtgaggttt
     1021 tttctgcaag gaaaaaataa acagaggtgg aaccacgtat aatacgtcct cttggcattt
     1081 gccaagagga tttttttatt ttaaaggagg aaaagaagat ggagtatttg attgaagtta
     1141 tgccgcaatt attagtagga gcactcacaa cattgaaatt atttttattt accttgattg
     1201 gctcattacc attagggatt gttttagcat taggattgtc tttgcatttt aagccgctac
     1261 gctacttgct tcaaacatat gtatgggtta tgcggggaac accgctgttg ttgcaactga
     1321 tttttatttt ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac
     1381 tcattgcctt tatcttaaac tatgcagctt attttgcaga gatctttcgt ggagggatac
     1441 aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa
     1501 cggtgacgaa aatcgtcatc ccacaagtga caaagattgt tttaccttct gtagggaatg
     1561 aagttatcaa cttggtcaaa gattcttcct tgatctatat attaggttta ggtgacgtaa
     1621 tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt actgcctttg ctaggcgtgg
     1681 caatcatcta tcttacattg actggtattc taacaatcgt aatgaaacaa gttgaaggaa
     1741 aattaaatta ttaccgctaa ctgataggag gaagaaatat gattgaattg aagaaagtga
     1801 acaaagcatt tggtgaaaaa aaaataatca aagatttgaa tctaatcatt ccagatggga
     1861 aaacattagc tatcgtaggt ccttccggtg gcggtaaaac cacgctttta cgtatcttgg
     1921 ctggcttaga aagttctgac agcggagaat tttatttaga cggtgtaccg ttcaacccat
     1981 tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt ccaagacttc cagctatttc
     2041 cacatctgaa tatttttgaa aacattacct tagcaccaaa aatggtattg aaacagacaa
     2101 aagaagaata caccaaaaaa gcagaagaat tagcggcttc attagggatt agtgatttat
     2161 tgaatatcta tccttatcag ctttctggcg gacaaaaaca gcgggtagcg attgccagag
     2221 cattggctat gaatccacgg gtattggctt atgatgaacc aacaagtgct ttagatcctg
     2281 aattacgcca gcaagtcgag cgtttgatcc tatcattgaa agaagatggg gtaacccaaa
     2341 ttattgtcac ccatgattta gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt
     2401 ccccaattca ttaagaaagg agaagataga gatgaaaaaa accagttttc tattcgcttt
     2461 gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag cagataattg
     2521 ggcgactatc aacgaagaga aaaaaatcgt tataggttta gacgatacat ttgttcctat
     2581 gggatttaga gataaagacg ggaagctcac aggatttgat attgatttag ccaaagcagt
     2641 ctttgaacaa tatgggatta cggtagactt tcagcccatt gactggtcaa tgaaggaatt
     2701 tgaactgaac aatggaacga tcgatttgat atggaacggc tattccaaaa cgccggcaag
     2761 agaaaagaaa gtgcaattta caaaacctta tatggaaaac gatcaagtgc tgatcacacc
     2821 taaagaatca ggaatcacaa actttggaca aatgaataat aagagattag gtgcgcaaaa
     2881 tgggtcatct ggttatgatg tatttacgaa acaaccagaa gtattaaaag agatagtcaa
     2941 aaaccaagat gctgttttgt atgatagttt taatgaagca ttgatcgatc tgaaaagcag
     3001 acgggtcgat ggtctgctga tggataaagt atacgcagac tattatttga aacaaagaaa
     3061 cgaactagaa gacttcaaca taacaaaagg ttcttatgag agtgaggatt ttgcagttgg
     3121 tgcacgcaag tcagatagta cattagtgaa aaaaatcaat gaagccctag atcaactaga
     3181 gaaaacaggt gaattccaaa aaatatccga taaatggttt ggcgaagatg ttacgccgag
     3241 aaattaacaa ataggggact gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac
     3301 agtgttctcc tgccaactcc ttgtgactcg gagctgaa
//
LOCUS       DACEMO010000213         3331 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEMO010000213 DACEMO010000000
VERSION     DACEMO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.219"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(99..719)
                     /locus_tag="IW728_002466"
     CDS             complement(99..719)
                     /locus_tag="IW728_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1025800.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(721..1590)
                     /locus_tag="IW728_002467"
     CDS             complement(721..1590)
                     /locus_tag="IW728_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1025801.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(1613..2020)
                     /locus_tag="IW728_002468"
     CDS             complement(1613..2020)
                     /locus_tag="IW728_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1025802.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(2039..3328)
                     /locus_tag="IW728_002469"
     CDS             complement(2039..3328)
                     /locus_tag="IW728_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1025803.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT          927 a          735 c          584 g         1085 t
ORIGIN      
        1 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
       61 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
      121 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
      181 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
      241 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
      301 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
      361 tgccataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
      421 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
      481 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
      541 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
      601 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
      661 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
      721 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
      781 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
      841 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
      901 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
      961 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
     1021 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
     1081 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
     1141 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
     1201 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
     1261 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
     1321 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
     1381 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
     1441 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
     1501 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
     1561 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
     1621 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
     1681 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
     1741 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
     1801 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
     1861 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
     1921 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
     1981 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
     2041 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
     2101 ctacatcggt tgctgtagca ttgttgaatc ccatcgaatt aaagatcgtt actagcaaag
     2161 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
     2221 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
     2281 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
     2341 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
     2401 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
     2461 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
     2521 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
     2581 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
     2641 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
     2701 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
     2761 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
     2821 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
     2881 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
     2941 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
     3001 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
     3061 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
     3121 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
     3181 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
     3241 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
     3301 tttcgatgat aaaattgata acgcccatgt t
//
LOCUS       DACEMO010000214         3292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEMO010000214 DACEMO010000000
VERSION     DACEMO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.220"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            37..588
                     /locus_tag="IW728_002470"
     CDS             37..588
                     /locus_tag="IW728_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ1025804.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1264..1530
                     /locus_tag="IW728_002471"
     CDS             1264..1530
                     /locus_tag="IW728_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025805.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1588..2100
                     /locus_tag="IW728_002472"
     CDS             1588..2100
                     /locus_tag="IW728_002472"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1025806.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2097..3134
                     /locus_tag="IW728_002473"
     CDS             2097..3134
                     /locus_tag="IW728_002473"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1025807.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1213 a          555 c          653 g          871 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta cacaaaaaaa
     1321 ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa tccgaaattg
     1381 agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa aaagctatcc
     1441 gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac aaaatttaaa
     1501 gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata ataagttata
     1561 ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg ttactctacc
     1621 gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg gattcgccaa
     1681 ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa aaaagctaga
     1741 attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc aagtgaagat
     1801 aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc ggaatattgc
     1861 cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg cttatctgcg
     1921 aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga agagaaaaaa
     1981 caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc ggaagccgct
     2041 gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga ggaagaatga
     2101 tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat cgcaatgggg
     2161 cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac gaacgatgga
     2221 cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct gtacccaaaa
     2281 ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa aaagaattta
     2341 tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt tatattgcat
     2401 cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac cgtggccgaa
     2461 gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca aatgaagttt
     2521 ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac cggttatatc
     2581 tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa tcagaagaag
     2641 ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc caaggacagc
     2701 cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt caagtcttac
     2761 tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat gacaatcctt
     2821 attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat aacggcttta
     2881 aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat aacgattctc
     2941 atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga cgacatctcg
     3001 ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat cctgaacgct
     3061 ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat cctttaaaag
     3121 aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct cgtcacccaa
     3181 taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca tagggaggtt
     3241 ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac ac
//
LOCUS       DACEMO010000215         3270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEMO010000215 DACEMO010000000
VERSION     DACEMO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.221"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(287..1327)
                     /locus_tag="IW728_002474"
     CDS             complement(287..1327)
                     /locus_tag="IW728_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1025808.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1676..2002)
                     /locus_tag="IW728_002475"
     CDS             complement(1676..2002)
                     /locus_tag="IW728_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025809.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1989..2777)
                     /locus_tag="IW728_002476"
     CDS             complement(1989..2777)
                     /locus_tag="IW728_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1025810.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          988 a          529 c          441 g         1312 t
ORIGIN      
        1 aggcttttca gcttggggaa ataagtgaat tacctctctt catgaattat tataactcaa
       61 aaaagcacat atgcaaacac tagttcccta tttttttgaa tgaaaaataa ctaaaatatc
      121 taacgattat gactttaaaa cctacgtttg tgattgtttt cataaacttt taatctaaaa
      181 actccggaat aatagctatt tatttgtaac atttgtaata aaattaaaat cataaaaaag
      241 ttctaattta aaattgatca tttttatagt gatcgctttt tatcaactac tctagccaat
      301 tttccgtact aactgttggt aattcgttaa atgtttctct ttgttcgttt gcaagctttg
      361 ccgcaatagc tttttcgaca aaccaatttt ttacataaac gaaaattaga ttctgcaaat
      421 tgtttacttt ttcagttttt tgtttttgat aagctttcag caaagtcata tataatcctt
      481 tgtcagcatc aattccataa gattccagtt cttcataaac aacaactgtt tgctctaatt
      541 cttcagcttt tttctttgca ttatgaatag tttggacggt tttttgagcg tcttcgtaag
      601 tactagaaaa tgtttttatc aagtttaata cattttctgg tacaaatgta tttatactgt
      661 cctctttcat gatgtcgacg aagttatcta gtaaaacttg attttgtaac tgatcagttt
      721 cagtgtctat tatgtgtcta ttagtgtcta tagaattaga atagaataga ttgtctgcga
      781 ttttcgcaat tacggttgag tccttgatat gactggattt tgccgtattt gcgattttcg
      841 caattacggt ggtatcattg atttctcgcg gttttgccgt atttgcgatt ttcgcaatta
      901 cggtggtatc agtattgtct tcctcttcca tgattttaaa gacgtcttga ttcgttactt
      961 ctggcttgcc taagtacaat aaattaggca tggattcaat cttaccgtca cgtttattta
     1021 ctcttcctct cttctgtttc agaagacctg ctttttctaa ttcctttttg attttagata
     1081 ctttaccttc gcggcattgt aacaatttca ttaattcttc aactgtgaat atgaaaaata
     1141 tattatcatc agaatctacc cagttatttt tgactgaata atcaaatcga tctttaagta
     1201 gcatataagc aatttttgca tcatttgata gatccatgta tttttcgtta gtaaaaaata
     1261 ccttaggcat ttgataatat ttttcacgat aaatttcttt tgatttgtaa taattaaaat
     1321 cattcatagc caatcctctt tctgtaggat agcgttttgt tcaaaactat gttacaatga
     1381 attaaacaaa tatatctatc cactatatat ttttctactg ctgataagtc gccaaacttt
     1441 gttaaagcag tagtcttttt ttatcttttt ttagtttacc ttaaaaaata aattaaagca
     1501 aaccgctcta tttcaaggtt taagctcata gtaactatat cacgatatta caataatatt
     1561 atatcacgat atcgtaatat tacaatatga agataagcga cttatcagct ttgatactac
     1621 tgatttttat aaaaaaaata tcacgatatt atgatatcgt gatattgaat gtaaattaaa
     1681 ttttattttg tttctttgct agtttatatc cttcaatcaa gtattcttca ggtatggact
     1741 ttaatagcgc atcttctata acgtctggtt tacttttgtc tgattcagca atagtcgtcc
     1801 aatatttgag tgctgacatt agaattggat ttatacgaat gctatctgct gttcttacac
     1861 tacttccttg atcttttatg actttactta tatttttctt ggttaattcg atttcttcta
     1921 cttcttttga tttaaaattc tttttatttt tatttcctag taatctactt actgcagctt
     1981 gactgcgatt atcttctgcc atcaaaatca ccaacttctt ttaaatattc ttctgtaaat
     2041 gctttatagt catcttcagc tgttgagggt ttattaaaat tgattagatc tgatccagca
     2101 gcttgcgctt cgtcaacggt tattgtccta cgaatataag ttttgaaaat ttttgcacca
     2161 atgactctag aaatttcggt tgctgcagac gtcatttctt tagaaatgtt ataacgctta
     2221 ttatacttag taaatagaac tcctcgtact tcaagcttgt cgttcgtata ttcttttacc
     2281 gtttcaatag tttcacttaa ctttattagt ccttttaatg agaaatacgc agcttcaact
     2341 ggaatgataa tttcatcaga aacagtaaat gcgttgactg taagcattcc taaacttggt
     2401 ggacaatcaa ttataatata atcatagttg tccttgattg gttgaagtac tcttttaagc
     2461 ctgtgttcac ttcctacttg agtaaattcc ctctctgcag aagaaagggc tatgtcggct
     2521 ggcaaaacat caattccttt atagtccaga atagcatctg taatgtcagc ctcccctttt
     2581 aatacttcat aaattgtagg ttggtttgtt ccatctatat cgaaacctac attatctgta
     2641 gcatttccct gaggatcaat gtccactaaa agtacatttt taccgtaaac aaatttcaaa
     2701 catgctgcta aattaacgct agtcgtagtt ttagaaactc cacctttttg attggctact
     2761 gacaatattc ttgccattaa aatatccccc attcatctta tattataata taattatatc
     2821 ataatattgt gatattgcaa tatcgtatat gcattatatt gtaatattgt gtgattattt
     2881 tgttttaaag atatatatcg gttcggatat aaagattatt catatgttat tcgaaattaa
     2941 aataatcact ttttattact ttaaaatttt ttattaatga taagaaatat gttttgcgag
     3001 cagcaactag aataatttaa acattactaa actgtctata ttgttactaa taattccctt
     3061 cttatagtgc attttcttag aaaaaaacaa tattacgata tcatgatatt aagatatcgt
     3121 aatattatac aattgtgatt gatattatat atgttttttt atttttttat catgtttaat
     3181 cttgaaggaa tcatattctt tttctaaaga caggagcttt tcataaacat cagacatgtt
     3241 taaactagag gccaggctta aatcataaac
//
LOCUS       DACEMO010000216         3268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEMO010000216 DACEMO010000000
VERSION     DACEMO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.222"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..270
                     /locus_tag="IW728_002477"
     CDS             <1..270
                     /locus_tag="IW728_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025811.1"
                     /translation="LHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTVS
                     HNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            437..838
                     /gene="tnpA"
                     /locus_tag="IW728_002478"
     CDS             437..838
                     /gene="tnpA"
                     /locus_tag="IW728_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1025812.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            850..1995
                     /gene="tnpB"
                     /locus_tag="IW728_002479"
     CDS             850..1995
                     /gene="tnpB"
                     /locus_tag="IW728_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1025813.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            2233..2496
                     /locus_tag="IW728_002480"
     CDS             2233..2496
                     /locus_tag="IW728_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ1025814.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            2490..2840
                     /locus_tag="IW728_002481"
     CDS             2490..2840
                     /locus_tag="IW728_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1025815.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(2864..>3268)
                     /locus_tag="IW728_002482"
     CDS             complement(2864..>3268)
                     /locus_tag="IW728_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1025816.1"
                     /translation="YDEIIASVNYLKKKNVQLIITSLPIMAEAIGNPLLDKFIKDLII
                     QILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQRRLVYKNIVEDL
                     KKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         1195 a          485 c          602 g          986 t
ORIGIN      
        1 ttacatcatg aagtaatcag acggattcaa aaagacttta gttgtatgga agaaatgaag
       61 tactttgttc aaaatgttga agaacagtta tatttggaaa gcgaaacgag tcaaacaaag
      121 gtcacagtca gccataatcg tttagatgtg tataaacgtc gtcctgaatc attaaaaaaa
      181 ggagcggagc ttctacgact agaaacagaa aaatacgtca tgaatgttta cctagaaaaa
      241 agtgagttgg tcgaaaattt tatggactag ccgagaacac ccgtgatttt aatcatggga
      301 tgaatcggat tcagttgtga cctagcttta gctaggttaa acaactgaca aatataaact
      361 aaataatagt gaaatgtctt gcaataattg attttgtaga aatacatata ttaaaataat
      421 tgcgaggtgt ttttgtatgt cagaaattca ttatggacgg ggttatgtct attctattca
      481 atatcatctt gtttggtgtg taaaatatcg acatgatgtt ttgtatggtc aggtagatat
      541 tgatgtcaaa cagtttttga aacaaattgc tgatgataat aatataaaaa ttatagaaat
      601 agaatcagat aaagaccata ttcatttact cattgaatgc accccacaac actatattcc
      661 tagtattgtt aaagtattta aaggcgcatc cgctagactg ctgtttaaaa aacatcctga
      721 acttaaacaa ctcctttggg gtggacatct ttggaatcca agctactttg tagctactgt
      781 tagcgaaaat acagaagaac aaattagaaa atatatacaa aatcagaaaa agaaatgagg
      841 tgaatccgta tgctacaaca tagggcttat aaatttagaa tctatcctaa tcaagaacaa
      901 gaaatattaa tgactaaaac tattggctgt tcaagatttg tctataatta ttttttgaat
      961 ttgtggaatc atgaatatac gactactgga aaaggattat cttataattc ttgttctgcc
     1021 atgcttccac aaatgaaaag aaatgcagag acaatttggt taaaagaagt tgacagtatt
     1081 gcgattcaat cctcattgaa aaatctttct gatgcttttt ctcgcttctt taaaaagcaa
     1141 aataaacgac cacaatttaa aagtaaaaaa catcctatac aaagttatac aactaaaaat
     1201 gtgaataata gtatcgaaat tagaaacaat ttcgtaaaac tacctaagtt aggatttgtt
     1261 aaatttgcga aaagtcaaga gcccaaaggg cgaatcttaa atgctacgat tcgaaaaaat
     1321 tcgagtggta aattttttgt ttccatcctg tgtgaagaag aaatagttga gtttcctaaa
     1381 tctaattccg ctattggaat cgaccttggt ataactgatt ttgcaatcct ttctgatggt
     1441 caaaagattg ataacaataa attcacgtca aaaatggcaa aaaaactcaa acgtgaacaa
     1501 cgtaagttgt caaaacgtgc attactagct aaaaagaaag gcgtcaatct ctttgaagct
     1561 aaaaattatc aaaaacaaaa acgaaaagta gcacgactac atgaaaaagt aatgaatcaa
     1621 cgcaatgact ttttaaacaa gttgagcaca gagattatca aaaaccacga tattatctgt
     1681 atagaagact taaatacaaa aggtatgttg cgtaatcata aactagcaaa aagcatttct
     1741 gatgtgtctt ggtctagttt tgtgactaag ttacaataca aagctaactg gtacggtcgg
     1801 aaaatcatca aagtagataa atggtttcca tctagtcaaa tctgttcaaa ttgtggacac
     1861 aaagacggca agaaatctct agaaattcga gaatggactt gtcctgtttg tcatgctcat
     1921 catgaccgag atatcaatgc tagtatcaat attttgaccg aaggtctaag attacatgtc
     1981 atgggattag cttagatcca atcatcaaaa accgtaggaa ctacggggat agcttggtaa
     2041 ataagagaaa ccgctgtgag taaagaaata cgcttgcaag tatgctctat tcccaagaat
     2101 ctcgtgactt tagtcatgag aggttcaagg attcatggaa tgttaatcta atttaataga
     2161 aaaggataat taagtatata cgttttttct tatgtatgtt atactatgta tatacagaaa
     2221 gaggtgtcta ctatggaaat gttacctata aaaaaatggg gaaatagtaa tggccttaga
     2281 ttgccaaagt atattatgga atatcttgga attcatacag aagacaaagt gaagataata
     2341 caagaagaag taaacggtca gaagcggcta attatcgaag cggctaatat agaaaatgaa
     2401 ttaacaattg aacagttatt tgaaaattac actgatgaaa gaaatcatgt gacgattcaa
     2461 aatttaggtg aagcagtagg taatgaaaaa tggtgagaaa acctagacaa ggcgatattc
     2521 tattattgaa tacagcacct cgatctggac acgaacagac aggtaaaaga ccttatattg
     2581 ttttaagtca tgatattatt gcggattata gtaacgttgt tacagttgct ccaatttcta
     2641 gtacaactag aaactatccg ctatatgtaa atataaactc agaatatgaa atgaagacaa
     2701 caggaaaagt attattagat caactaacga caattgatta tgaagccaga gagtgtattt
     2761 ttttggaaaa agcgcatgat agtttagttg aggagttatt gatgaaagtt agaacagttt
     2821 ttcaaaaagt aagcaaataa aatagaaaca gcagcatgat caactattcg tgattgacca
     2881 tgctgtcttt ttttattcta tatacggttt gtctcgtaac attgtagtct tttgcaatct
     2941 tagcgatcgc aacacctttt tttagatcct caacgatgtt tttataaaca agacgacgtt
     3001 ggggatcttt tgcattggca ctgtatagct taggacgtcc tttataaaca ccgttcgctt
     3061 ttgcaatctt aatgccttgt gcctgacgtc gtttagattc ggtacgttct tgttcagcga
     3121 tcatggccaa aatttgaatg atcaaatctt taatgaattt atccaggaga ggattgccaa
     3181 ttgcttcggc catgatcggt agacttgtga tgatcagctg cacatttttc ttcttcaagt
     3241 aattgactga agcgatgatc tcgtcgta
//
LOCUS       DACEMO010000217         3260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEMO010000217 DACEMO010000000
VERSION     DACEMO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.223"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            177..788
                     /gene="mobC"
                     /locus_tag="IW728_002483"
     CDS             177..788
                     /gene="mobC"
                     /locus_tag="IW728_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ1025817.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            773..2164
                     /locus_tag="IW728_002484"
     CDS             773..2164
                     /locus_tag="IW728_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1025818.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            2380..2694
                     /locus_tag="IW728_002485"
     CDS             2380..2694
                     /locus_tag="IW728_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025819.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            2687..3181
                     /locus_tag="IW728_002486"
     CDS             2687..3181
                     /locus_tag="IW728_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025820.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
BASE COUNT         1266 a          449 c          617 g          928 t
ORIGIN      
        1 cgcatgattt cttagaactt gacgaattat gtgaatcagt aaagaacgca acaaatgaaa
       61 aaatcacggt cactaattat attgaactga tgattcaaga agaattaaag atgaaaaatg
      121 aaatagagag caatttcaaa tgttgctctc tagaaaaaaa tgtgagggga taatcaatgg
      181 atgataaaca aaaaatgtac gaaaaaaatc gcaaacatag tgaggcctta aattttcgtg
      241 tcgataaaaa gacttatctt gaactgacat tgattgcaaa acaaagggga atttctagag
      301 cagacgtttg ccgtgagcgg ttaaagggac tcaaatatga accgccgttg ttagctgaag
      361 atgatagggt agttttaacc aaacagttaa atcaaattgg taacaatttg aatcaagcgg
      421 taaaggcatt aaatatcatt gctaaatatt ttagagaaga atctgaagat aaaaaagaaa
      481 agagcctaca ttttagtttt gatgctagcc gttacaacat ttttaaagat gaacttgcga
      541 atagcatagc aaaaaagttg atgattagtt atccagatga catttttttg gagtccaatc
      601 ggagtgcccc ctcttttttt aaattatcag aagtagaaaa agaatatttc gaatattgga
      661 tagaaaagca aactagaccg ggtacgaaag aagagtattc ccaaaaagtt gatcggaatt
      721 taattcactc aattcagatt attgcaagtt taaaagatga ggtgaaacaa ctttgggagc
      781 tactataaca tcgaaaggac gcaagaaaaa tttatcctcg ctattacaat atgcagctaa
      841 tccttcaaaa aaagaaaaat gtgttgcagt ctcaggaata aattgtagtg atgatagtga
      901 ggaagcactg caagaaatga atttagtacg taccaagatg aataaatcta aagatatagc
      961 acaagcgttt catgttattc attcgtttga taagagtact tctaaagagt taagtattga
     1021 taaaatgcac gaaatagctg tcgaatttgc agagaaagct tttccaaatt cgcaaatcat
     1081 tgttgcaagt cacaatgata aagatcattt tcatagtcat cttgtcatta ataatattaa
     1141 catggaaaca tgtaaaaggt tgcgtattga ccctaaagat ttagaactaa tgcgaaaaat
     1201 taacgatcaa gtcctcaatg aacatggtct tgaatcagtg aataatgagt actatgaaaa
     1261 cttagaaaag agaaccaatc tttattcaaa cgatccttct aagatgaaat atggaaaaat
     1321 taattatcaa gagtttgtgc aaaatgcgat cgaagcaacg ttggaaaaat gcgaagtgaa
     1381 ttcttttgaa gatttttcgg atcatttggc aaaagaatat aacattgaag tttatcaatt
     1441 ttcaaaaaca aataacaaac ttggctatgt tctttacaaa caaggtgtaa atttctatga
     1501 gcgtagaggt gagttaatta ctttaggaag agataaacaa tctagaaaag aaaaaagtca
     1561 gtatgtcgaa cgtactttta gtgcaagaaa gttagggaaa agatattcat ttgaatctgt
     1621 ttctgaacaa cttgaaaaga atttagaaga atcacatata aaaaattttt ttaatatgga
     1681 aagagagcaa gttatgtcgc taattagtgg agtaaattac aaaactggtg agttaaatgt
     1741 accgtttgtg gaattagatt ccatcgcaca aaagcaattt agcgaccgtg aaggcgatga
     1801 aaaattcatt aaatatgatg acttcttgcg agaggtagac aagcattttg taaatacaat
     1861 gtctttagaa gatgatcaag aatacacgat taaacttcat acggatgtga atcctcaaac
     1921 tttacgtagt gcgaaaagta aaacaatttc tattaaaaag actgatcaga aagagttatt
     1981 tctaccggca aagggttttg aaaattgttc tcatacacct tctgctaaac agattcacta
     2041 tcaaatcagt cgcataatga atgacgatgt aaaaatgtat cgtgctaaaa aggattatga
     2101 attcacacaa caaatgattg aaaatcagca aaaaaggcaa caaaattttg aaatggaatt
     2161 ctgaatgggc ttgttagtgg actttccagt tttttgccta tgaagagttg cttggaaaga
     2221 agcagttttt aggacatacg ccagctaccc atctacttgc cagtcaatcc gccccgctca
     2281 cgctaggcta ctttctggca agtcagatgg cgggtaaatc tctgagggag caagctccct
     2341 acagactccc tcccaaaaaa agaaaggtgg aagaaaaaaa tgaactatcc atattttaaa
     2401 gtctcggctt cagaagaaac taaagaaata ttcaacaatt tttacaacca aaacaaagga
     2461 atttttggtt caaaggcaaa tatgtttcgt gttatggttt caaatttacc agtgttagct
     2521 agtccatcaa ataataaatt taatgatcct gaaagcatta aatttgaaca aaaaatttca
     2581 gaactagaat caatgataag taatgaagta attgaaaagc tggatgacat tgatcaaaag
     2641 ttatcgtatt ctctaaaaaa caaatacaaa acggaggaga aaaaagatgt ttaaagttta
     2701 cttcccgttt cagtggctcg ttaaaggaat tcttttagtg ctactaggat tgattctttt
     2761 tggaagttat ctaacaatta aagccagaca ttttccagaa gaaaagccaa taaaaattgt
     2821 aacaaaagat ttaaaaatag gtgaaactta tcaggcagat ggggttgaac taacatttaa
     2881 aaatattgaa gtgaccgaaa aaagaaatga atttgcacaa catgatcgtg tattacaagt
     2941 gacctataca cttataaatg atacagaaaa tccgatacgt ccacaacaat ggtcgattga
     3001 aaataaggat ggtgaagaac tttatgagta tgatatgata ttggataatc cagagtatat
     3061 tgaaagtggc aaagaagaaa cagtaaatta ctattatgaa ttaccgattg aaaggcagga
     3121 ctaccaaatc gtagtacgta gagagaatgg ttacatagtt gggttgccat ttactttata
     3181 agttttattt tgttagtagt ttttaaggta atatcagttt tgtgattttt gaaaatggaa
     3241 ctgggagaat ggctgaggaa
//
LOCUS       DACEMO010000218         3217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEMO010000218 DACEMO010000000
VERSION     DACEMO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.225"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..1438
                     /locus_tag="IW728_002487"
     CDS             50..1438
                     /locus_tag="IW728_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1025821.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3217
                     /locus_tag="IW728_002488"
     CDS             1776..>3217
                     /locus_tag="IW728_002488"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1025822.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEE"
BASE COUNT         1182 a          505 c          582 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaaga
//
LOCUS       DACEMO010000219         3167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEMO010000219 DACEMO010000000
VERSION     DACEMO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.226"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..582)
                     /locus_tag="IW728_002489"
     CDS             complement(<1..582)
                     /locus_tag="IW728_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1025823.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLI"
     gene            complement(709..1182)
                     /gene="dfrF"
                     /locus_tag="IW728_002490"
     CDS             complement(709..1182)
                     /gene="dfrF"
                     /locus_tag="IW728_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1025824.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(1601..1819)
                     /locus_tag="IW728_002491"
                     /pseudo
     CDS             complement(1601..1819)
                     /locus_tag="IW728_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1850..3064)
                     /locus_tag="IW728_002492"
     CDS             complement(1850..3064)
                     /locus_tag="IW728_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1025825.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT          987 a          686 c          467 g         1027 t
ORIGIN      
        1 aattagtcca caaatatctt tattacacca ataatcaatc agccaaattg ctttttcatt
       61 gtcgtttact acattcaacg attctaagta tccttttctt tctttcggta tttttctttt
      121 caattcatca taagataacc gttcaagcat tttctcagtg ctttcccata cttcgaaaat
      181 atataaatat aattcttcca gattaagctg ttttgaaaag tctgcaatct gctgtttcat
      241 gagttcattt cctgttgtaa tgataggtga attgatacgc tcttgataat tgcctgcaaa
      301 aaatacttgt tcatcttcat ttatcactgt atgcacaact atatcttcaa tgcggaaaat
      361 atgccaaata gaataagcaa tcgtcttact atgataacca tcagcgttta tgaaggggat
      421 tgagttaaaa tcttctctgc ataattcttc tttgaaagat actaaggttt gtattaactg
      481 acttcgtaaa gtaagtaaag tatcaatacc cctcttataa gtatctttct tttttatttg
      541 tgcctgcatt gttttattaa gttcagacca ttccttattc atattcaatc ctctctcaaa
      601 ttctgatttt atcgccccat acaaactgga atttgtctta caacaattta tatatgcata
      661 aaaagcaata tatttatgaa aacaccctaa atccaaaatt tactcaattc attttttcct
      721 tacataaaat gttctcgtat atttcacttc ctcaccaagt gtttccccta tcaaaacttc
      781 aaaatcattg atatcaaatt ctggaaagaa tgtatctcca tcttcaatat ttaaatctac
      841 ttctgtgata tacattttat ctactatttg caaagcttcc ttaaatagtc catatccacc
      901 agatatgtat acatctcgtc ctttagccaa taataatgca tcttctaatg atttaactga
      961 aactaaatta tctccttgat actctgttgt ggtggaaaca acaatattca ttctattagg
     1021 caacggatga ccgatttctt cataagactt tcgccccata ataaccacat tacccgttgt
     1081 taactctcta aattgctttt gttctccctt tattttccat ggtatattac cattcttgcc
     1141 tataacatta ttctttgacc tcgcaacaat caaacctatc atctatttca tcctccataa
     1201 tatgatgtcg tacctgtgat tccatcaagt gcaccacatc ttaacaaact caaaattatc
     1261 tcgaactaac cagctcgaca aattcctttt ttgtcttcat ctgcattata tcatattgtt
     1321 ataagcattg ccatttcttt tctgcttttg aaaagttact tatcagccta tgttttgtaa
     1381 agggtgatat tcagcatttc tcacgcaaca agcccttgac aaaccataga ctaataagtt
     1441 ttaaggtaat caagcagaaa tgaaatagtc tgctgtttca cggcaacaaa aaagagatac
     1501 caacacactt atgtagtatc tcttagtcta gtagcaatat tcttttgttt gcaagcaggt
     1561 aaacatgggc tgtttcttaa tatctttttg tttgggaaac acctttttcc catcctttca
     1621 ccgttgccat ctctgctgcg cacaatgcca cagggataaa ccatagcaaa ccgcctaata
     1681 acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt gggatattca tataatgaaa
     1741 cgaccattga gatggtcata gccaaaaagc caaagaacgt taattttttt gcaggaattt
     1801 tcgttttgct ttgttgcatg aatcttcctc ttttctattt gaatttgttt caattgagtt
     1861 tgttgccaaa gaagaaaaag tccaatcctt cttcttgttc ttcttttgta gtagtgatcg
     1921 ttgtaaagtg catcgccgat tttaaaatca ctgttgtcag cacataggca tacacggcag
     1981 taatgatgac accaatcact tgaatcccta agaagtacca gctgcttgga ttcgttagta
     2041 aatggctgtt agatgcaaag atccctatca gaatcgtacc tgtgaatccg cccatgccat
     2101 gaacacccca cacatcaagt gcatcatccc attgtttctt tttacgaaaa tcaacagcag
     2161 catgacagac gatacccgca atgatcccaa tcagcatggc actttttgct tctacataac
     2221 cggcacaagg cgtaatggct gccaatccag caacggatcc tgttagtacg tcgacaaaat
     2281 caaagccatt tccttttact tttgcaatga tcagccaagt aatcatcgca aatgcaagag
     2341 cgatgaatgt tgaaccaaat gcattgactg cttgttcatc tgctcgaagt gcaccaccgg
     2401 aattaaaacc gaaccagcca aaccaaagca gtcctgtacc gatagcagct gccataagac
     2461 tgctatgctg acttctttct cctaaaggaa tctttctttg accaagtgtg aggatacatg
     2521 ctaaagagcc gaatccagct gtcgtgtgga tgactgctcc accagcgaaa tctctaaatc
     2581 ccatctgatc caaaaatccg ccaccccata tccagtggca aacagggaaa taaatcagca
     2641 gattccaaaa aataacaagt ataatatagc cttttaaatt caatctccct gcaaaagctc
     2701 ctgtcatcaa aggaacggta atcacgataa acatcagttg gtaaagaaag aacattaaaa
     2761 atggaatcgt tgcaccatga aacatgttgg ggaagcttgt aacatgtctc atcaaaaaga
     2821 aatctgccgg gtttccaata acaccgccga tatcccgtcc aaaggccaat ccgaatccac
     2881 caaataccca cataatcgtt gttatcccaa cggagagaaa agattgagac atgatcgtta
     2941 gtatattatt cttccgtgct aatccgccgt aaaaaaaagc tagaccagga gtcatcaggc
     3001 aaaccattgc tgtacataaa atcatgaatg ctgtatctcc tgtattgata ggtaatgtat
     3061 tcattttttt cgcctccttt ctacgattac gagtataaaa gaaggtgtag ttgttttttt
     3121 cttctgtttt tttgaattaa tacttgcatt tataaaagac agacaat
//
LOCUS       DACEMO010000220         3133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEMO010000220 DACEMO010000000
VERSION     DACEMO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.227"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            208..1041
                     /locus_tag="IW728_002493"
     CDS             208..1041
                     /locus_tag="IW728_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1025826.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1143..2843)
                     /locus_tag="IW728_002494"
     CDS             complement(1143..2843)
                     /locus_tag="IW728_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025827.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
BASE COUNT         1110 a          520 c          623 g          880 t
ORIGIN      
        1 gaagcagcag tttgaatact gtttagtgga gaggtcgtga aaaagctgcc ctgcaccaag
       61 tgataaaaac agccaaacaa aaaatagctc ctggtccatt gagcttatta ctgaggagtt
      121 agcaggagaa cactatttgc tggagaggtt gtgacaaaaa ttttgtcaca acccttttgt
      181 ttcgaaatga agacaaggag aaaaccaatg aaattcatga cactcaacac gcatagctgg
      241 cttgagccag aaccagagaa aaaattgcaa gaattagcag ataagatcct gttagaagat
      301 tatgaagtga ttgctttaca agaaatcaac caattactgg aatctgaaga agtagaacct
      361 gcgaagctaa tgaagttctg tccggtaaag aatcaagtac ctatccgtaa agataatttt
      421 gcctaccgtt tagttcagct tttaaaggaa cacggaaaag agtatttttg gagttgggaa
      481 atgagccaca tcggttatga taaatatgaa gaaggaaatg cactgcttac gaaaaaaaca
      541 ttggaaagtc aggtccttac cgtctcccaa tttcaaaaga aagaagatta tcagacacgc
      601 aagatcttga ttgggaaaac aaagatagat gatcaggaca tttttgtctc gagctgccac
      661 ttttcttggt ggacagataa aaaaagtggc ttttattttg agtggaaaaa cttggaaaac
      721 tactttttgg aaactcgcgt accacttttt ttcctaggtg atttcaataa tccagtagac
      781 tctcaaggtt attatactgt aagagaaagt tgccttcttc ttcaagattc gtatgttgtc
      841 gcgaatgaaa aaggaaaagc agctactgtg gaaaagaaaa tagatggttg ggaacaaaat
      901 acagaaaaat tgcgtataga ttttattttc gtccctgaag gaatgcaggt taaaaaatat
      961 caacggattt ttgatgggat cgatagtcca atcatcagtg atcactacgg ggtggaaatc
     1021 gaattagatg tgaacgaata aaaaatataa catataacat aaaaaaactg attaaaataa
     1081 aactctccaa acgattatac cgtttggaga gttttttgat cggatgtatt tatccctttt
     1141 gcctaagcac gtaaaagctt gtatctatga taaataaata aacatagcaa ggtaaaccag
     1201 ccaatcaaag taagagaaag aatgaaatag aaatagattg gcgtttgata atgaagcgtg
     1261 agctcgttga ttcctttttg ctgcttcacg attggggtcc cgatatctgt tacttcataa
     1321 tcagtcaatg tttttccatt ttgctgtagc acagtccgat cgtagacgat aacagggata
     1381 tgaacagttt tgtccccagt gtttttccac ttgattgtca aaagaccgcc tggatgtgtt
     1441 tttataaaag ttgtttcgtt ttctagtatt tcttcgttat accggtcgta tttattgtct
     1501 tttgtctcct tatagattgg cagatagtca ggtgtggttt tttggaaaac gaacaagctg
     1561 gtagacatat ctttgctgaa gaatgttttc cttgcttctt ccggcgtatc gaatagataa
     1621 gtatgtcgac tgacaaatgt ttcattttcc cacttttcca gatgctggta taagttctgg
     1681 atcgtctgtg ctgaaaaaat aagaatgcca aatatactta aaagacggaa ccacttttgt
     1741 tcagaccact ggttcagtga aagggcaagg taaagtagaa gcaatagcgt aaatggcacg
     1801 aaaaatcgaa atggaaactg gatcaaacta ataaaaggca catttttttc gatcaatgtc
     1861 gtccatggaa tcaagctagt agaaagaaaa aagaaaacaa gacttgtgta caacacgatt
     1921 ttttggaata aggtgttctt ttgttgaaag aaaataaaaa gcaaaaaacc tgataatatc
     1981 aatataggaa aggccttagg gtaatggagc caataactgc catccagatc tatcgccttt
     2041 tgactcattt ctctgttaac aaagggagga agaatcttat tcccactgta aacaatagct
     2101 aatcctgccc aaatatttgc tgtcagaaca aaataaagca agaccgactt aatcagttga
     2161 agcagtgctt ttcctttttc agtctggtta aaaaagtaat aaagataaaa agggatatac
     2221 atcaaagcta acatgatcga agacagtaaa tggatctgtg tcataagtcc cactgagaca
     2281 gcgactttga taacagggaa ctttttatga aggacgaaat cagcaatagg gatcatacat
     2341 aaaggataaa aacaagtccc ccagctagtg aatccttggt tgatagacca gtattgaata
     2401 gaatacgtac tcatgaataa aacggctact ggtacactta cccaatttcg aacattgaca
     2461 tatctcagta aaatcagcat agaaaatcct gaaataagat aaagaatgaa atttgaaaga
     2521 atctgataat gaaaccagct acctgcaagc aacacgatca atccttgtaa ataagcgaaa
     2581 aatgggccgt aaacggcatt cacgatcctg ccactttgct ggaatccata aagggaagga
     2641 aaataatgga aatccccata tttaatttgt tgcgcggcat catagaaccg attgtaatgg
     2701 aatggggcat cagcacccaa aattagttta tgtgtgaata aatgaggcat gagaaatgcc
     2761 aaagcaatga aaaaaattgc taaaaatggg ccatattttt tcaattgtcg aactcctttg
     2821 caaactttag ataaattcat catatatcct atgcgagagg taggcaagaa attagaaaat
     2881 tcatgaaaat atttatgaat tttacaagaa aaacataagt gaaagttcta aggagagagg
     2941 atgcgacata agttttgttg catctccttg ttttgaaaca gaatttttct taatactcga
     3001 aacaaagtag ctttttcatg acctctcggt aaacggtgtt caatcagctg cttctcggta
     3061 ataagctcac tagcctagaa aatatgagaa gctattttct gactagcgct cttattactc
     3121 aaagcataac gca
//
LOCUS       DACEMO010000221         3101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEMO010000221 DACEMO010000000
VERSION     DACEMO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.228"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..225)
                     /locus_tag="IW728_002495"
     CDS             complement(<1..225)
                     /locus_tag="IW728_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025828.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEW"
     gene            599..1570
                     /locus_tag="IW728_002496"
     CDS             599..1570
                     /locus_tag="IW728_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1025829.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            1602..2999
                     /locus_tag="IW728_002497"
     CDS             1602..2999
                     /locus_tag="IW728_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1025830.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
BASE COUNT         1102 a          505 c          662 g          832 t
ORIGIN      
        1 ccattccatt tttttatctg taaatgtcaa ctgtcccgcg gataaatcca tatctttacg
       61 tacttttagc gtatatacac tgttggtggt atctgctttt tccgctttta agacagaaga
      121 atcggttgtt tcgtccgagg attggtcaga gccgccaaac aaatcacagc cagtcaatgt
      181 caaaagtccc gcagacaaaa taaatagtac gattaatttc ttcaaaatac ttcccccatt
      241 tgataggata aagaaattat accattggaa tgtaaaactt caaacggatg gaagagaaaa
      301 acgcacaaaa cacccacaaa tagagtaaat tttagaataa aattttaaaa aggcaaaaaa
      361 tccgccactt tcagaaagcg ttttaaaaaa gtgttgcaat cttttaaaaa aatgctatat
      421 taaaaatgaa ccggttcaaa tacttgttta agtataatta atagtgagag gaagttgctc
      481 tactgaagac tttagttata tggatattga tgacaagcaa cactatgcaa agaatgcggt
      541 catcataatc gaacaacaag aaagagaaat caaatgtaac tggcaggagt gagaatgtat
      601 ggcatcgatc cgtgatgtgg ctaagatggc tggtgtttca gtgggaacag tttcccgtta
      661 cttgaatggt cagcaactga aggaaaagaa catgaaaaaa atagcagaag cgatttcagc
      721 gttggactat aaagaaaaca tcattgctaa agggttgaag aataatcgaa gcttctctat
      781 tggacttttg ataaacggga tttccagccg ttttgggtcg gaagttgtct cagggatcga
      841 aagagtagct gaagaaaatg ggtatagctt attattaagc gggtttgccg atcagccaga
      901 aaaaattgaa caaaaaattg aatacctgat gaagcatgtg atcgatgggc tgattgtctt
      961 cttatccgaa gaagagtggt ctggatttga aatgctcaca aaaatgagta ttccagttat
     1021 cgcattgaat tgtccagaag gaccagcagg tattgataca atattagtga atgatcgaga
     1081 aagtgtagca aaagtgatcg ctcatcatgg tgaacaagga cataaaaaaa tcggatttat
     1141 tgctgccact caaacagact acgtagcaag agaacgttta gcaggagcca aagaagcggt
     1201 ccaaaatgat ccatcgatcc aattagaggt ctttacagga gactattcta gaaaaagcgg
     1261 ctattacggg gcaaaagcat tattagaaaa aggggcaact gctatttttg tcagcaatta
     1321 caatatgtcg attggtgcca tcgaatgttt caatcaagaa ggtgtcagaa tcgggaagga
     1381 tattgcgttc agtcactatg attatttaga taaaaacgat ctgagtatcc ttccgaaaat
     1441 cacgatcacg ccaccaacag ataaaatcgg ggaacgagca gcagaacagc tgttaaaacg
     1501 aatcaaaaaa gaagaggaag aaaatgaaaa agtgattgtg atggaaaata tcatacatgg
     1561 aatcaactaa aacgaactaa aaaagaaaaa gaggtgcttt gatgaaaaaa tatcaatttc
     1621 cagcaaattt ttggtggggt tctgcagcaa gtggcccaca aacagaagga aggatttcag
     1681 gcgatggaaa aggagagaac atttttgatt attggtacaa aaaagaaccg caaaagttct
     1741 ttgatcaagt aggacctgaa aaaacctcgc aagtttatac gaagtatcaa gaggatgtac
     1801 agttgatgaa acagactggg cataattcat ttcgaacatc tatccaatgg agccgtttga
     1861 tacctgatga atctggaaca atcaacgaag aagccgttcg tttctatcat cgttattttg
     1921 atgagttgat tgaaaacggt atcgaaccct ttgtgaatct gtatcatttc gatatgccga
     1981 tgtatctgca agaaaaaggc ggatggttga accgagaaac ggttgaagcc tatgaaaaat
     2041 atgccaaaag ttgttttgaa ttgtttggtt cgaaagtaaa aaaatggttt actcataatg
     2101 aaccaatcgt tccagtcgaa gcaggttatt tatatcgatg gcattatcca gaagagtcag
     2161 atatgaaaaa agcgatccaa gttggctatc atgaagcgtt ggcttcagcg ttagccatca
     2221 aatcttacca tgaaatggag caaggtggtg ttattggtat catcttgaat ctgacaccaa
     2281 gctatccaag agacgaatca aaccctgaag atgttaaagc agcgcaaata gcagatgcgt
     2341 tcttcaatcg ttcattcctt gatccagctg taaaaggcga gtttccaagt gaattgattg
     2401 agatagtaaa agaacttgat ctcatgcctg tgatccatgc agaggatttg acggttatca
     2461 aagaaaatac agtagatctt ttaggtatca attattacca gccacgccgg gtaaaagcaa
     2521 aagaaacacc aatcgatact aaaaacggtc caatgcctga ggattatttc gacaattatg
     2581 aaatgcctgg tcgtaaaatg aatccttatc gcggatggga gatctatgaa caaggaatct
     2641 atgatatttt gatcaatgta cgagatcatt atggaaatat cgactgcttt atttctgaaa
     2701 acggtatggg tgtagaaaac gaagcacgat ttatcaaaga agatggcatg atccacgatg
     2761 attatcggat cgaatttgtc caatctcatc tgaaatatgt ccatcaagcc attcaagaag
     2821 gtgccaattg tttaggctat catatgtgga catgtatgga taactggtcg tggaccaatg
     2881 catataagaa tcgatatggc tttatttcag tagacttagc acaagatgga aaaagaacaa
     2941 tcaaaaaatc gggtcattgg ttcaaagacg tatcagataa aaacggattt gaggcttaag
     3001 aaagaatagt tgagagcaaa tgcgtgaaat tctccattct taccattttg tgaaaaagaa
     3061 gttcaattat ttacgatctt tttcaacttt tttgtctttc t
//
LOCUS       DACEMO010000222         3096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEMO010000222 DACEMO010000000
VERSION     DACEMO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.229"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2177)
                     /locus_tag="IW728_002498"
     CDS             complement(<1..2177)
                     /locus_tag="IW728_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025831.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3096)
                     /locus_tag="IW728_002499"
                     /pseudo
     CDS             complement(2849..3096)
                     /locus_tag="IW728_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          856 a          696 c          506 g         1038 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatc
//
LOCUS       DACEMO010000223         3083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEMO010000223 DACEMO010000000
VERSION     DACEMO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.230"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            332..400
                     /locus_tag="IW728_002500"
                     /pseudo
     CDS             332..400
                     /locus_tag="IW728_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            1105..2967
                     /gene="ltrA"
                     /locus_tag="IW728_002501"
     CDS             1105..2967
                     /gene="ltrA"
                     /locus_tag="IW728_002501"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1025832.1"
                     /translation="MENVSASHSIRHMEYYGMIDSFDNLYKESRDDHIFNNLMEIVTS
                     PNNILLAYRNIKGNSGSVSPGVDNKNIDDLKEIPNADFIRIVQGKFSEYKPQPVKRVE
                     IPKPNGKNRPLGIPTIWDRIVQQCLLQVLEPIMEAKLHDKNYGFRPNRSAHHALAQAV
                     RLAQLSKLTFVVDIDIEGFFDNVNHSKLIKQLWTLGIQDRWLLGVIRAMLKAPIIHKD
                     GRIEHPKKGTPQGGILSPLLANVVLNELDWWISSQWETHPTRHNYDCYQANQGYWNKG
                     NKYRALRRSKLKEIYIVRYADDFKIFCRRRSDADNIFIATKLWLKERLKLDISLEKSK
                     VVNLKKKKSEFLGFTMKLVRKRKTFVIETHMCPKAMKKVSDNLTKQIKVIQHTPTKED
                     LQKQLVKYNSMVIGIHQYYRRATHISVDCPKINQRIRIIIKNRLDVKKNGSPPSDFVK
                     KNYARSKQLRWINGYPMIPLGFVKHSHTSLPKMGICKYTPEGRVLIHKYLTFPIDIIN
                     QLMNMNNPDESIEFRDNRVSKYSAQRGRCRVTKELLELEDIYCHRIIPKNLGGKDNFQ
                     NLVIIHQNIHQLIHENDRTRASLLITRMRITGAQLEIINQFRKKAQKCELSLNL"
BASE COUNT         1083 a          532 c          625 g          843 t
ORIGIN      
        1 atgatggagg aaatagtatg agaatttttt gtaagtgtct actgataact tattctcaaa
       61 tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa aaagtccgta
      121 ctctaatttg cagtctctca ctcttttaga gtgagagttt ttattagatt ttcaaattaa
      181 ttagtaaagt gatagaataa attgatcata caaatcaaaa gaagaagcag ggtgatagca
      241 tacctatgct tcttttttta tgtatttagc tatttcttct ttacaatatc gaacatatgt
      301 ttgtataatg tgaaagaatg gagtgttatt tatgatggat tattcaaaag agccagtaaa
      361 tgattacttt ttaatcgata tgaaaagctt ttacggtgcg aaacgttcct aactgaaaag
      421 gttaggcaca caagactttc ttgtgtagaa ctaatgattt gaggagtgaa atgacggtgt
      481 aacgacctga agcgctccct ctgatactcc gattggcaat acctttagtt agaataggaa
      541 ttgtcaaaag ctcggtaaag tcggctgaga gttaccgtag ttgcattatg caatcgaaag
      601 ctgttcagcg gtgaatggtg taacctatag gtcgggagtc cagaaattac tcatggtgag
      661 aatgtgcagt tgcactgacg aactcgcgaa tgtacgaatc tataagcttg gggtgcagaa
      721 atgtatctta cccgcacaaa tattactgtg acaaagggtg tagcttgcgg ttaagtaaga
      781 atcgtagata tgaacaaccg tgcaatgtgg tagacattgg aaagttacag gttcagagtg
      841 agcacctaaa agtaaatgca gggataaaat cattggaacg ttgaaagcta gtgtcgtgga
      901 ggactaactt cctttgaaac ggttgagacg aagaataata agctcattta cactagtgaa
      961 agtagtggca cagtacctat gaagcagtga taataaactg tagagggata gccacaagtc
     1021 aatttactct actaacagta atgatataat ccatagaatc gagtatgaca aaataaacgc
     1081 cgacgtccga aaggaggcgg tgctatggaa aacgtatcag cttcacattc aatccgtcat
     1141 atggaatact atggcatgat tgacagcttt gataatctat acaaagaaag tcgcgatgat
     1201 catattttca ataaccttat ggaaattgta acctcgccaa ataatatctt actagcgtat
     1261 agaaatatta aaggaaattc tgggagtgta tccccaggcg ttgataacaa aaatattgat
     1321 gatttaaaag aaatacctaa tgcggatttt atcagaatcg tgcaaggcaa attttcagaa
     1381 tataaaccac aaccagttaa aagagttgaa atcccaaagc caaacgggaa aaatcgccca
     1441 ctcggcatcc caactatttg ggatagaatc gtacaacagt gtctactaca agtcctagaa
     1501 cccattatgg aagcaaaatt acatgataaa aattatggtt ttcgtcctaa ccggtcagca
     1561 caccatgcac ttgctcaagc agtacgctta gcacaattgt ctaaacttac ttttgtagta
     1621 gatatagata ttgagggatt ctttgataat gtaaaccatt caaaactcat taaacagcta
     1681 tggacattag gaattcagga cagatggctt ttaggtgtta ttagggcaat gcttaaagca
     1741 ccgatcatcc ataaggatgg gcgaatcgaa caccctaaga aaggaactcc gcagggcgga
     1801 attctttccc ctcttctcgc aaatgttgtg ttaaatgaat tagactggtg gatatcatca
     1861 cagtgggaaa cccaccccac aagacataat tacgattgct atcaagctaa tcaggggtat
     1921 tggaataagg gtaacaaata tcgagcgtta cgtagatcaa agttaaaaga gatctatatt
     1981 gtgagatacg ccgatgactt caaaattttc tgtagaagac gtagcgatgc ggacaatata
     2041 tttatagcta ccaaattatg gctaaaggag cgcctgaagc tcgatattag cctagaaaaa
     2101 tcaaaagtgg ttaatctgaa gaaaaagaaa tccgaattcc ttggtttcac tatgaaattg
     2161 gttcgtaaaa gaaagacctt tgtcatagaa actcacatgt gccctaaagc tatgaaaaaa
     2221 gtatctgata atctaacaaa acaaataaag gtaattcagc acacccctac caaagaggat
     2281 ttacaaaaac aactagtgaa atacaactcg atggtgatag ggattcatca atactataga
     2341 agggctaccc acattagtgt ggattgccca aaaattaacc aacgtataag gattatcatc
     2401 aaaaatcgat tggacgttaa gaaaaacgga tcaccgccta gtgatttcgt taaaaaaaac
     2461 tatgcacgtt caaaacagtt aagatggatt aatggctatc ctatgattcc tctaggattc
     2521 gttaaacaca gccatacatc gttacctaaa atggggattt gtaagtacac gcccgaagga
     2581 cgtgtgctaa tacacaaata tcttaccttc ccaatcgata ttatcaacca acttatgaat
     2641 atgaataacc cggatgaaag cattgaattt agggataatc gtgtctctaa gtactctgca
     2701 caacgaggca ggtgccgagt aacaaaagag ctcttggagc tagaagatat ttattgtcac
     2761 cgtatcatac ccaaaaatct tggtggaaaa gataacttcc aaaatctagt tatcattcat
     2821 caaaacattc atcaacttat tcatgagaat gatagaacga gagcttcact cctcataacg
     2881 aggatgagga ttacaggcgc tcaacttgag ataatcaacc aattccggaa aaaagctcag
     2941 aagtgtgaat tgtcgctgaa tctataactg tatgataatt gttagtaaac attgatggaa
     3001 cgccgtatgc ggtgaaagtc gcacgtacgg tgtggagcgg gggaaaatct ggagattata
     3061 tcaaaggatt acctatcgct atc
//
LOCUS       DACEMO010000224         3081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEMO010000224 DACEMO010000000
VERSION     DACEMO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.231"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            78..1085
                     /locus_tag="IW728_002502"
     CDS             78..1085
                     /locus_tag="IW728_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1025833.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1097..2101
                     /gene="neuB"
                     /locus_tag="IW728_002503"
     CDS             1097..2101
                     /gene="neuB"
                     /locus_tag="IW728_002503"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ1025834.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2098..>3081
                     /gene="neuC"
                     /locus_tag="IW728_002504"
     CDS             2098..>3081
                     /gene="neuC"
                     /locus_tag="IW728_002504"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ1025835.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIE"
BASE COUNT         1160 a          391 c          588 g          942 t
ORIGIN      
        1 taattagttt agttatatat tttttaaaga ttaagaatcg aattatagat gactataatt
       61 cataggagga gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt
      121 ccattggaag tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt
      181 atagtagagg tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt
      241 tgagattttt aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg
      301 tggatattat ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta
      361 tggaagctgt aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa
      421 ataatgtaaa acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca
      481 tgggtgcgac aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa
      541 aaacagaaac aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg
      601 ttattcctct ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg
      661 atatgacacg atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta
      721 tgaattcaaa atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt
      781 tagctcaatc attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa
      841 taaaagttgt aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg
      901 aaattgaacg ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta
      961 ctgacgaact gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa
     1021 cagaattaat gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt
     1081 ggtaaggagt tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca
     1141 taatggaagt ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa
     1201 cgcggttaaa tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc
     1261 aaaatatcag aaggataatc ttaaagagga cagttctcaa tttgaaatgc taaaaaaatt
     1321 agaattatct caatctgatt ttattagaat aaaagattat tgtatacaaa ataaaattga
     1381 ttttttgtct actccttttg atgaagaaag tgcagacttt ctaaaacaaa ttggtgtaca
     1441 aggatttaaa attggctcag gagatttaac aaatttacct tttcttagaa aaatagatat
     1501 gtatgggttg ccagttttat tatctacagg tatggctgat attcatgagg tcagtgaggc
     1561 tgtagattgt ttcatagata gtccagttac tattttacat tgtacttcaa attatccggc
     1621 tagttctgaa gatattaatt tattggcgat taacacatta aaagaaatgt tcgggatacc
     1681 cgttgggtac tccgatcatt cattgggata tgatgttggt atttgtgctg ttgcgatggg
     1741 tgctaaggta attgagaaac attttacctt ggataaggaa ctacctggtc cagatcataa
     1801 agcttcttta gatcctcagg aatttgcaga ttttgtaaag catattagaa acactgaaat
     1861 atttttaggt gatggtgtta agcgagcaat gccttctgaa cttagtacaa aagaagtatc
     1921 aagaaaaagc ctagtggtca ctgagaattt aaatgtggga gatattctta cagagaaatc
     1981 tcttacaata aaacgtcctg gaaatggaat agctcctaag tttttatatc aatatattgg
     2041 caaaaaagtt aaaagaccaa taagtgcaga tagtgttctg cttgaggaag atgttgaatg
     2101 aaaaaaatac ttatagtaag tggaacacga gctgattatg gaatatataa acctgttgct
     2161 gagaatttac tacaagataa ttgggatgta tcatttgttg tttctggaat gcatttatcc
     2221 cataaatatg gatttactaa agaattgatc ttagctgacg gatttagaat tagtggagaa
     2281 gttcaaagtt tgtttttaga gaacaataag ggaaacatgg ctcggtctgt ggcattagag
     2341 ataaatggtt tcacaaatat atttgaacaa actagccctg attttgtatt attactaggg
     2401 gatagaggag aaatgttggc agctgcgata gcttgtttgt atcttggaat caaaacagca
     2461 catattcacg gaggcgaagt gtctggtact gtagatgaat caataagaca tgccataacg
     2521 aaaatagcat ctgttcattt tgctgctacg gaaaaaagca aagagcgttt aataaaaatg
     2581 ggggaagatt cttggagagt ttttcccgtc ggagctccta gaatagatac tattttaaat
     2641 aatagattac ctagttttag agatataaaa gaaaaatatc atctaaaatt tgaaaagaaa
     2701 aaatacgttt tgcaagtatt tcatccagtt gtaaccgaac tagaaaatat agaaaaacaa
     2761 attttagctt tgatcaattc aatggaacaa caatctcaac caattatttg cattttacct
     2821 aatagtgatg ctggtagcga aattatcaga aaaatgtacc agaaatattt aaaaacggat
     2881 attatttata ttacgaattt agagccggag gaatacctga ctatattaaa agagtgcaaa
     2941 tttatgatcg gaaattcttc ttctgggata attgaagctg catcattcaa aataccagta
     3001 ataaatttag gaaccagaca aaatggacga gaacaatcta aaaatattat aaatgcaaat
     3061 ctaaatgaaa atgaaattga a
//
LOCUS       DACEMO010000225         3055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEMO010000225 DACEMO010000000
VERSION     DACEMO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.232"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            787..>3055
                     /gene="esp"
                     /locus_tag="IW728_002505"
     CDS             787..>3055
                     /gene="esp"
                     /locus_tag="IW728_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1025836.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         1116 a          445 c          592 g          902 t
ORIGIN      
        1 tatcaagtta tttattcttt ttgatacatg atgaatttat ggattatcaa aaattggatc
       61 agttaattga aagagaaagc tgggaagatt attacgtggg tctaactttt tcagaaaagt
      121 taaacaacta caagttaata gattataagt atttgagtga aagcaattaa ttattcatgt
      181 agagtactag acactttaaa tatggacaat tcaattataa agaaaaagaa tcaataattt
      241 aatgaggctg tgacaaatgt cattttttat ttcgctaaaa acgagtgaaa aaatgttgat
      301 aactccaatc attcgtttaa gattctagga atcattcaga aatgctgtga aaccgatact
      361 ttataattat aaagaaggtg atttcaaatt ccgaagaagg aatttgaagt caccttttta
      421 cttatgtcac agtctcattc agtaatgcgg gataaggctc cttattccgt tatttgaata
      481 ctctgaagtt taatctaaga aagggtaata ttttgaaaac atgcttcatc gcatagaaat
      541 gaacttaatt tcagaaagaa tacatacata tctttttata tgttttcagc tgctgaatgg
      601 ttaaaaagca caaatatccg aaaaaaacat gaagaaatgt actttttttg ttgtaatgtt
      661 catgaaattc tctaagtaaa ttagttaaca taaggttagt aagattccta ttctgtgaat
      721 aatacgtatc agatttatct tttattctta ctaggaaagt aattattaag ggagttgatt
      781 tgaataatgg ttagcaagaa taataagaga gtatttctag aaaaaacaaa gaaacgagta
      841 ttgaagtaca gtatcaaaaa attaagtgtt ggggttgcat ctgttttgat tggtgttggt
      901 cttgtcttgg gaacaactga actagttaaa gcacaagatg aaatttctcc aagcagccca
      961 cttgaaacag cgataagctc cgtacaagta ggtgacaaag tagcttcggg gaatactttt
     1021 caggagaatc ctggttatac gaaaaattat aacttttcgg atttacaatt tagtcctcaa
     1081 gaactaactg gagacacact aaaaggcaat acaattggat ttgaagttta cggaaaacat
     1141 aatattgcag catcaactaa aaactgggaa atccgtcttc aattagatga acgattagct
     1201 aaatatgtag aaaaaattca ggtcgatcca aagaaaggaa ttggatcttc tagacggacc
     1261 tttgtaagaa ttaatgattc gctgggtaga cctacaaata tttggaaggt caattatatt
     1321 cgtgcaagtg atggattatt tgctggggca gaaacaaccg atacgcaaac cgctcctaat
     1381 ggggtaatta cgtttgaaaa gagcctagat gaaattttta aagagatagg catagacaat
     1441 ctcaagaccg atcgtttaat gtatcgaatc tacttggtaa gtcatcaaga cgatgataaa
     1501 attgtaccgg gtattgatag tacgggctac tttttaacag attcagatga tttctataat
     1561 agcttagatg tatctgaaaa taatcctgac cagtttaaac atggatcagt aagtgcaaaa
     1621 tatgagaaac ctaatactca aacaaaagat ggttcaggat ccacaggtgc taatggagct
     1681 ataatcttag atcataagtt aacgaaaaat tataatttct catattcatc ttccgcaaag
     1741 ggcacgcctt ggtatgctaa ttacaaaatt gatgaacgat tagttccata tgtagcgggt
     1801 atacagatgc acatggttca ggctgacaaa gtaacatatg atgtttcgtt tgaatccgga
     1861 acaaaagtag ctgatttagc aatcgagcga cgggaaggtc atgaaaatta tggcataggt
     1921 tcaataacta ataatgattt aactaaactt atcgattttg ctaatgcaag tccacgtcca
     1981 gtcgttattc gatatgtttt acaattgact aagccattag atgaaatctt agaagatatg
     2041 aaagcaacag cacaagttga agaaaataaa ccatttggtg aagatttcat ctttgattct
     2101 tggttgtcgg atacgaataa aaaattaatc cagaacactt atggaacagg ttattattat
     2161 ttgcaagata ttgatggtga tggaaaccct gacgataaag aagagagcgg agacacgaat
     2221 ccatatatcg ggaaacctga attagaagaa gtatatgatg ttgacacaac agttaagggg
     2281 aaagtattca tccacgagtt agcgggaaca ggtcacaaag cccaacttgt tgataaagaa
     2341 ggtactgtat tagcagaaaa aactatcgct ccaaatgaaa aagatggggc tccaatttca
     2401 gatactgtag aatttgaatt tacgggtgta gattcaagta aattaatcgc gaaagatgaa
     2461 ttaaaaatcc aaatcgtttc tccaggtttt gataaaccag aagaaggttc aaccgttatt
     2521 aaggaatcac caaaagcggt tgataaacaa accgtggtag ttggatttaa accagatgct
     2581 aaagaatcaa ttcggaataa taaaaactta cctgaagatg cagagtattc atggaaaaca
     2641 gagcctgata cttctaacgt tactgatagt acgaaaggta ttgtaactgt taagatcgga
     2701 aatcgaactt tcgacgtgga tgtagagttt gctgtaaaag cttctcaagc tatggaaaat
     2761 gatgcaacat acgtacctat aacaacaact ccagaaacga cagttcaaag tggaaaacct
     2821 acatttgata aaccagatgt tcctttagct aaagatgcct tttcaatttc agatgtctat
     2881 aataaggact tcggtaatgc aagtgttgat gcaaacactg gtattgttac attcactcca
     2941 gctaaaggtg taggagaatc ggagccgatt actggaacaa ttcctattaa aattgtttac
     3001 caagatggtt ctgtaggcac gaccgattta gcagtaactg taagtaaaga tattt
//
LOCUS       DACEMO010000226         2989 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEMO010000226 DACEMO010000000
VERSION     DACEMO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.233"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..92
                     /locus_tag="IW728_002506"
     CDS             <1..92
                     /locus_tag="IW728_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1025837.1"
                     /translation="KAVMVNETARKAIDLHIENLRREIRRLEE"
     gene            89..394
                     /locus_tag="IW728_002507"
     CDS             89..394
                     /locus_tag="IW728_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025838.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            394..750
                     /locus_tag="IW728_002508"
     CDS             394..750
                     /locus_tag="IW728_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1025839.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            710..955
                     /locus_tag="IW728_002509"
     CDS             710..955
                     /locus_tag="IW728_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025840.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            976..1065
                     /locus_tag="IW728_002510"
                     /pseudo
     CDS             976..1065
                     /locus_tag="IW728_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1140..1607
                     /locus_tag="IW728_002511"
     CDS             1140..1607
                     /locus_tag="IW728_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAQ1025841.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            1882..2118
                     /locus_tag="IW728_002512"
     CDS             1882..2118
                     /locus_tag="IW728_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025842.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            2142..2483
                     /locus_tag="IW728_002513"
     CDS             2142..2483
                     /locus_tag="IW728_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025843.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(2476..2655)
                     /locus_tag="IW728_002514"
     CDS             complement(2476..2655)
                     /locus_tag="IW728_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAQ1025844.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
BASE COUNT         1087 a          424 c          635 g          843 t
ORIGIN      
        1 caaaggcagt gatggtcaat gaaacggcga gaaaggcaat cgatttgcac atagagaact
       61 taagaaggga aattcgtaga ttggaggaat gagcgtggat aagaaagcga caatgaaacg
      121 aattgctgaa ttaaccaagt cagaatcttg gcaggaagac aaagaaatag ttgcagaagt
      181 ccaaaagctc ggtaaatcaa tgtggactga aaagcctaaa cggaaaacgc cgagaaaaat
      241 tgcaatctgg catggtgatc gaattctagt aacaggtaca gctgaacagt tatctgaaat
      301 tactggatta agcaaaaaca ttatctggga tagagctagg agcttatgga ttgattcaaa
      361 aggacgacag tttaggtatg tggaggagag ataatggatc tcattacaca atacagtgac
      421 atcatcctca agaaaatcat gatgaagatt cagaaagaca aaaaatcaaa agaacgagct
      481 gaattagtta agttagaaat ggctgaaaca ggagcaggag tgcgaagtag caggcattgg
      541 aaagcagcag caaacattga attttattac aacgaaattc aaaaagggtt cgatcagatg
      601 cgtgagctgg atcggcaaac aaattggagc aagaaacttc atcaagatcg tttcaaattt
      661 gtagagaagt ataaagaaat attagaagag tatttgagga ggacagcaaa tgataaaaaa
      721 actcgttcaa ttcagcatgg atttatatga tatcgaatca ggagcaacac tatctgtgga
      781 atcggaccat ctaatcataa attttggtgg aaagcgccag attattttgt gggtagttga
      841 tgatgtactg tttccagaaa ttgttcatga tttcgaagaa tcaaaagcgg ttgagtttga
      901 aatagtgaaa aaagtaatgg aattgattga aaaatacgag gaggacagcg aatgataccg
      961 aagtttagag cgtgggatag agaaaaagac agaatgaagg cagtagcttt ggttgaattt
     1021 gctgaggcgt ataaatcagg agaactcatt tcacgtacaa aataaaaaaa gtcggattcc
     1081 tccgactatg aataatattt ccgacataag tattatatca taattggggg aatcagagga
     1141 tggtactttt cgatgtaaag aaatatgaga caccaagcgc gaaagatgtt gatatggaac
     1201 gcacaaaaca taatgtcgct gtgtttcttt cagcatatct atcagctaga tgtagagtag
     1261 ggcaacctcg agaaccaaaa gtgacagcat cttattcctt ggttccacct tctacagctg
     1321 atcatatatt tgaagcagaa agaatgctga tcgataaaga agaagcacag gaagaatttg
     1381 agtatctgca taaattgttt gttcgaggct attcagcgat acagcatccg cataagcctg
     1441 atgtgactga aaggcgcaag aagatattct atgatcgtta tatcaatggt ctgcccattt
     1501 atgtaactgc tcaaaggaat aatactagcg aagaatcggt taaagtagaa tcaaacagaa
     1561 ttatcatcca atttgcttca tcgttagaac tggttgcttt caagtagcca gtttttacac
     1621 tttttatacc cttttattac caatttggtt tccattttat acctttttta taccaatcac
     1681 ttacctattc aacgttgtat tatgatagtg tcgaaagatt aggaaacagg atcgacaaaa
     1741 taaaatgtaa gggaggaaat ctccctcatc gtttaattaa gcttcgatag gcagcagcgg
     1801 aaatattaag aataaggatg tgaatttcaa ctccttctaa attgttctta ttatctatca
     1861 tccgttgctg tctattaatt tatgtattgg agggtatata aaatgaagat cgcttccgaa
     1921 aaaattgatc aactaacaat tttagtaaac caaagaatcg aagatattat caaagaattc
     1981 gaaggtgaaa agattaagaa cataaagctt actttaatgg atcataagtt cgaattaaat
     2041 tcgacaaaaa ggaagatcgt tgagcttata gaagaagaaa tagctctcca gatcaatgag
     2101 caacaattag ctaattaatt tatgtattgg aggggaaatg aatggaagtc gaacaaataa
     2161 agtatcaagg tatggatcgg gaacggttga ttgatattat tgagcaacaa cgtttagaat
     2221 tgatccaaaa agaagctgta tgtaaagagt acaaaaaaca tttggaacaa gtaattgagt
     2281 atcattcagt agaaaagtac agatcagtgg tgcaaaaaaa tagagcaacg agtcctgatt
     2341 ctcgtcaaga tgtatcgatt agtatcaaga cgccagtatt taattctgtt aaatcagaaa
     2401 agctagaaag tatctcaact gctttggtag aaaaagcagc tgaaatgaac tccctagcaa
     2461 atgaagttag tcgtcttatt tagtcatatc gataacttga gagtctttgg tgattccgtt
     2521 ttttatagaa ttaatggttt gttcagcgga atatttagtc aagtatgttt cgctagttgc
     2581 tactacttca ttgttattgg acttaataac gaagtaatat tggccattag ttgcttctct
     2641 tataacaaag tacaaatttt tcaccaccta taatttattt cagcggacca ctcgctgata
     2701 attaaaatta taagctatgt ataaaatttc acaatatcaa tttgtcgctg tggcggaaag
     2761 ggtagacgca ataaaggatg aatgggaaaa gtcaatgagt aattatctga aatttacaag
     2821 tcccctgttt agttatctaa gattatttga taatattatt tctgaggtga tagaaaatgg
     2881 tgggcgtagt taaatttctt gggtttacat ttgatggata tggtacgtta ggcgaatggg
     2941 taggtattat tttgatagtg attacaattc aacaaacatc aaagtacta
//
LOCUS       DACEMO010000227         2968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEMO010000227 DACEMO010000000
VERSION     DACEMO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.234"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(84..1031)
                     /locus_tag="IW728_002515"
     CDS             complement(84..1031)
                     /locus_tag="IW728_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1025845.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1277..1741
                     /locus_tag="IW728_002516"
     CDS             1277..1741
                     /locus_tag="IW728_002516"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1025846.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1803..2846)
                     /locus_tag="IW728_002517"
     CDS             complement(1803..2846)
                     /locus_tag="IW728_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1025847.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          981 a          587 c          567 g          833 t
ORIGIN      
        1 tttattcgga aaaaaggact gtgacaagac ttttgtcaca gtcctttttc atttaaaaaa
       61 atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg cttcactttt
      121 tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat tttcccaata
      181 tatttcccga tgattccaag acataacaat tggattccac caatgaacag gaagatcgag
      241 accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa aataatgaac
      301 aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc aatcgttaac
      361 ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt ccagaaggac
      421 caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc tgttttaaat
      481 ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc taggacggca
      541 tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat ttctgtatcg
      601 ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg
      661 ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt gatccggttg
      721 atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac ggtcactaaa
      781 tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa atttctggaa
      841 aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc tgctaacgta
      901 ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc tttgaatttt
      961 tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg gacaacaatt
     1021 gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta cattcaaaat
     1081 ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac aggtattctt
     1141 tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact tttaagaaca
     1201 atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc cagtctattg
     1261 actaggagca aacaaaatga aaatacacac acctatcggc tttttatggt tagatgcaga
     1321 tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat cagcgaatca
     1381 gcgaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga
     1441 atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg tctggaaagc
     1501 tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg ttgctgttga
     1561 tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga accaatccat tgccaatcgt
     1621 tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata tgggaaacgc
     1681 aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc tgcaaaatta
     1741 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
     1801 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
     1861 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
     1921 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
     1981 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
     2041 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
     2101 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
     2161 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
     2221 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
     2281 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
     2341 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
     2401 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
     2461 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
     2521 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
     2581 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
     2641 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
     2701 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
     2761 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
     2821 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
     2881 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
     2941 aaagtcttgt acgacctctt gtttcgaa
//
LOCUS       DACEMO010000228         2935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEMO010000228 DACEMO010000000
VERSION     DACEMO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.235"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>2935
                     /locus_tag="IW728_002518"
     CDS             <1..>2935
                     /locus_tag="IW728_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1025848.1"
                     /translation="HREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQKIIS
                     NNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQLGSV
                     SLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELKQVTA
                     KDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKFLFSW
                     RTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAIQALF
                     IAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTTEFTK
                     FASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAALKYAK
                     KQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTPIFPE
                     NRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRK
                     LKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDNAYTK
                     FDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTMEDPD
                     FPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARK
                     RAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKT
                     KGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSM
                     ESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEEQIGV
                     YETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREE
                     IEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNE
                     WFVSEK"
BASE COUNT         1026 a          505 c          625 g          779 t
ORIGIN      
        1 tcaccgtgag gaagcggaag tcgaagaaca gttgatccgt gtattggggg aagggcataa
       61 tcagtggacg tatcgtcccg atctaaaatc agaagaagat ctttgggtca atttacgcca
      121 aaaaatcatc tcaaataatc aagcagaact gaatgatttt cctttgaccg ataaagaatt
      181 tgaaacgatc aaaacagaat tgttactacg aacgaaaacc ccctttgatg ctgcgaaatg
      241 gttaaaaggt gaaaatggaa tggctcggat cacgattgaa cgggaagatc cgcaactggg
      301 ttctgtctcg ctcattcttt attctaatca agacattggt ggaggaatct ctacctatga
      361 agtggtccat caaattgcaa aacgtggaag taacatagaa gcacgggatc gacgttttga
      421 cgtcacctta ctgatcaatg gattaccgat tgtccaaatc gagctgaaac aagtaaccgc
      481 aaaagatagt ttttatcagg cgtttaacca aatcaaaaaa tatgcagaag aaggtatgtt
      541 tcggaacaat atcttttcga ccttacagtt gtttgtggtt tccaatgaac aaaccactcg
      601 gtattttgca aatgccttac caaaggattt gcatcctaag tttttattta gttggcgaac
      661 gaaagataat aaaaaggtag aaaacttata tgagttttgc aaacaagtgc taaatatccc
      721 ggatgctcat cgcttgattg cggattatac gattgtcagt gaagatcaag acaataaaat
      781 cctcatggtg ttacatcctt atcaagtaca tgcgattcaa gccttgttta ttgctgcaaa
      841 taaacatcaa tcaggatatg tgtggcatgc gactggttca ggaaaaacgt tgactagttt
      901 tgtatctact aagcttttag cacgaaaatc ggggatcgat cggacgatca tgttagtgga
      961 ccgcaaagat ttagataatc aaaccaccac agagtttacg aaatttgctt cggaatttaa
     1021 tactgggatt tcttccggga acgccaaagc gaatagcttg atcgtgggaa caggaagtgc
     1081 caaagagtta agtgaaactc tgttggcaga tgccaatgca aatgttgtga ttattacaac
     1141 ccgtcaaaag ctagatgcgg cgttgaaata tgcaaaaaaa caagaagaga aaaaaggaac
     1201 caatcgcttc caaaaattaa tggggcaaca tatcgtgttt gtagtagatg agtgtcatcg
     1261 agcgttaagt gctgaaaata tggaagaaat caaaaaaatg tttccgaaat ccacttggtt
     1321 tggctttaca ggtacaccga ttttccctga gaatcggaaa caagccaaag ggcaattggc
     1381 acgtacgacc catgatcagt atggagaggt cttgcatacc tataccatca agaatgcatt
     1441 agaagatggc tcggttttag gatttcaagt agagcatgaa aacaccgtgg agccgacttc
     1501 attagagaat aaaatctatc ggaaattaaa agaagtagaa acatatgcag agtattcacc
     1561 ggaacagatc aatcgtatga ttgatcaaat ggagcctgtc aaaaaagaaa gttatttgga
     1621 ccctactgtt tttgaagcag atgaacatat ccaaaaagtc attcataaaa tgttccgtcc
     1681 agacaatgcc tatacaaaat ttgattttcg aaatggccgt ccaacgaagt ctgcgatctt
     1741 aaccacgagt tccattgaca tggccaaaaa gtattatcga gcgatcaaag aaatgacgaa
     1801 agaacctgac tggttaacaa aagagtttcc taatcagccc attcgtgaag gaagaacgat
     1861 ggaggatcct gattttccac gaatcgctat tacctattca ttagaagaaa acagccgaga
     1921 ttcaagtgtc caacaagaag aaatgcaaaa aattattgag gaatataatc agtattatga
     1981 tacctcctgg tcattagcag acattgagcg ttataacgga gacatcaata atcgtttggc
     2041 acgaaaaaga gcggaattca aacaatttgg gaaacaaatt gatttagtga tcgtagtgga
     2101 tcgtctattg acaggttttg acgcacctac gattcaaacc ctatttgtgg atcgtaatct
     2161 agagtatgca ggattgatcc aagctttttc acgtacgaac cgtacgtatc cagaaaaaac
     2221 aaaaggacta attgttactt tccgaaagcc agccacaatg gagaagaatg tcgaggatgc
     2281 gacaaaactg tattcagaag caaaagaaga atcgggtttg atttatccaa gttatcagga
     2341 atccaaaaaa agattcaagc aagcatatgg taagttaaaa gaggtttcat ccatggaatc
     2401 cattgatgaa catacgccac ttgaaatccg tatcgagtac gtcaaagcat tccaagaatt
     2461 gaatcgtgct tatgaatcgt tagtgactta tgacgattac aatgatgaca tggtgaaatc
     2521 ttcaaccttg aacaatcaaa ttcaactttt ggaagaacaa attggcgtgt acgagaccgt
     2581 gaagggttca ttaatagagg aagaaccaga aaaagaggga gaagattttt ccacgatcga
     2641 attttatagt gaaaatacca cgaagttgta tgatattgat tctacttata ttgatcaatt
     2701 actaggaact tatgcggcga atagttcaga tgcacgagaa gaaattgaaa aagctttggc
     2761 taaattgaat aaaacagaag gagtcaaaga agtttaccga caaattttaa atgcgtttga
     2821 tgtggggact ttagatagta atgaagatat ctttgtcatt aaacgtcgct attttacaca
     2881 agcgagtgat gatttgattc atgtcttttc caatgagtgg tttgtttctg agaaa
//
LOCUS       DACEMO010000229         2907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.236, whole genome shotgun sequence.
ACCESSION   DACEMO010000229 DACEMO010000000
VERSION     DACEMO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.236"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(820..1161)
                     /locus_tag="IW728_002519"
     CDS             complement(820..1161)
                     /locus_tag="IW728_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1025849.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1161..1391)
                     /locus_tag="IW728_002520"
     CDS             complement(1161..1391)
                     /locus_tag="IW728_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1025850.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1678..2016
                     /locus_tag="IW728_002521"
     CDS             1678..2016
                     /locus_tag="IW728_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025851.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2221..2460)
                     /locus_tag="IW728_002522"
     CDS             complement(2221..2460)
                     /locus_tag="IW728_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1025852.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2436..2783)
                     /locus_tag="IW728_002523"
     CDS             complement(2436..2783)
                     /locus_tag="IW728_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1025853.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT          921 a          525 c          449 g         1012 t
ORIGIN      
        1 atagaaggta ttgtaaataa taatattgac aaaaagtgac tcaaaataaa aaaaacctta
       61 cattatactg gaatgaactg tattgatagt gtacctaaaa gctctactga cataagatcg
      121 tgtaatcggt aaagtaattt tattaattat acgggatgta atcaattaga gaataaacta
      181 atcataaaag gtacttttac attcataatt gtcgctttga gattctactt tggttcacta
      241 tctgacccaa ttgcacattt tcgatcatta caagtattgg tgtcgttaga acaactaaat
      301 aaaaactagc ggtagatttt tcagaatcta tgctaatttc tgtatggatg ttccagtgat
      361 ggcaaatttc tcttttcatt gtttttcttg tcgcatgata tgcctttttg tgttattcag
      421 ttaaactata gaaattcttt cctagacaga tcttgatccc aaattttacg tttttgagca
      481 tcgatatagt ttaaaatgac attactcata tctaaagagt ttagattttt taggactaaa
      541 actgtcagtt gttctttttc ttctttctgt taaagtattg tcgcgacttt tcgtatacgt
      601 taagatatgt cgtgaaaaaa caagttttgt gacacaaaaa aacgaatgac ttgaggaaat
      661 cacctcaatc attcgtttaa aatgctatat attctttagt ttaagatacg gaaattaatg
      721 ccatcgacca cactagtaga atgaacgaca tttacttggc ctttttttaa tttcgttatg
      781 tttagcatat aaaaatatta tagagtttac ttacaatgct taaaacatat actgtataat
      841 ctgatcaatt ttagccatgt cttgcaatgg taagttttct atttttttga gttttcgttc
      901 cttaaaatca agagatttta attgtgtaat aattacttgt ccttttatat ctagctcttc
      961 aggtaatgag taacgtgtcg gtaaattctt tatagtggaa gtaattggac atatgaccga
     1021 aaacattgtt ttacggttaa attcataacg agagacgact agtcctggtc gtcttttttg
     1081 aatttctttt cctacggagg gatcaaaatc aatccataca atatcacctt ttttaggaat
     1141 ataaattctt tcttctgaca ctataaaatt tctctccctt caggagttaa atcttcccat
     1201 tcgtctttct cataatactc ggcctcttcc ccaccactaa atggatcttc aatttttgga
     1261 actaacgtaa ttgttccatc atcagaatac acaacgatat attcttggtt ttctgaaggt
     1321 ttctttccgt tatcagaagg taaagttacc actacagaac ttccttgtaa tctagatttt
     1381 gtaacaagca ttatcatcac tcccttaata aaagtatact atataacact aagtaacgca
     1441 aaattcccct ttgtaaacta tctagaaaat tgttttgttt aaagtatatc ttattggata
     1501 taaatagaaa taaattgtag ccagttcatt ttttctttaa ctaaaaaaat aatccaagaa
     1561 acattgaacc tatggatcca aaagctaccc aaatactata gctaaccccc cgctaccttc
     1621 aattttgcag ataaaacgct ttgggagaaa atttcaaaga caacaggcca tcctatcatg
     1681 cccgattata aacaaaaagc tggctattcc ttttgctctg acaaatttca attgggaaag
     1741 ttcacgaaac tagcaggtga agaagtttca actgttcgga tcaaagaatg cccgcttcaa
     1801 atagaaacat tggttcaacg aatttcaact agacaagact ttgctattgt agaacgtgag
     1861 atcgtagcta tttttgtaga agaagggatt ttacatgatc caactcatat caatgtggat
     1921 aaatggcaac ctcttatcta taaattcaga gaatatacaa ctgccaacca gtcgttaact
     1981 ttgaatttta gattccaaga attcaaagag tcataatgat aaacatcgtt aggtataaat
     2041 aaaaaagact cgcagcacga gtaaaaaaca aatagaagct atacattaga aaggtcgact
     2101 gtgaccctat agatgcagta tatgcagctt atgagcattt cttctgttct ctttgttttt
     2161 gggatctgtg ctgaagtctt tttcgttttt tgccgctctt tctttgaaaa atcgattatg
     2221 ttataccttc tccattacta attcaggcat ctgctgagta atgatttttc gcgcaaaaga
     2281 aatatctgtt acccaaggtt cgatttgttc ttcagtcaag acaagcggca ttcggtcatg
     2341 gattggtgcg actgtgcggt taggttcagt agtcattatg attgattctg ccattttttc
     2401 atcctgatgg ctacgataaa aaccgccaat atacactatt ttttccttat ttgaaaacat
     2461 aaatttgtct ttttcttcat tccactcatt gggaaaacac aacgattttg caaaaatggt
     2521 tgatgaaagg tctttttttc ttctactgtc tctgcacggg cattaatcat caattgtcct
     2581 tttttaaatc cggtaaatcc ccacttcatg atttccggag tgaggatgtt ttcttcctct
     2641 tttcttaacg tcacaacaag atttgacgga aaaacttctc cagctgctaa ttctgctgcc
     2701 tgcttcggtt gtttcgtctg ggcttctcga taataattct gaaggagctc tgactctaga
     2761 tcaaaaaaat aacgaccaca catcctaatc cctcccaatc tttttttcta tcatacactg
     2821 caacaagaag ttcgcaccaa caaaacgctt tcaatttttg tagaactaaa aaagttgtga
     2881 caagtgacaa actctccagt gaatggt
//
LOCUS       DACEMO010000230         2876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEMO010000230 DACEMO010000000
VERSION     DACEMO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.237"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            240..656
                     /locus_tag="IW728_002524"
     CDS             240..656
                     /locus_tag="IW728_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1025854.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            670..1236
                     /locus_tag="IW728_002525"
     CDS             670..1236
                     /locus_tag="IW728_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1025855.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1259..2245
                     /locus_tag="IW728_002526"
     CDS             1259..2245
                     /locus_tag="IW728_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1025856.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2400..>2876)
                     /gene="scm"
                     /locus_tag="IW728_002527"
     CDS             complement(2400..>2876)
                     /gene="scm"
                     /locus_tag="IW728_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1025857.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          855 a          537 c          608 g          876 t
ORIGIN      
        1 ttgaaaagta attttcgtaa attccttctt atttcttgaa gttgcccact tctgtcacaa
       61 cctcttgtta taaacggtgt tccacctgct gcttctcggt aataagccca aagaattaga
      121 aaacatggaa agctgttttc tattcttcgt tcttattact caaagcaaga cacaggtgtc
      181 acaacctctt gtttttatgc taaaattggg tgagttaatc tggaaaaagg agaacaaaaa
      241 tgagccttaa agaatttgct gaaaaaagtc tgctcaaacc ttgggatgtt tttattattt
      301 tgcttttgac tgttctttct tttttaccag tcatgatttt tggtcttcag caaaacaccg
      361 caacagcaga aaaagaagcg gtactgcgtg tggatggcac agaaatcaaa acattccctt
      421 tgatggctgg agaagagagc aacacatata cttacaaaga tgaacacgga gattataact
      481 tgatcgagat agatggcgat cgagtacgga taaaagaagc agattgcgat gaccagatct
      541 gcgtacgtcg aggctgggca acgaaaaatg gcgaaaccat cgtctgtttg ccacataaat
      601 tagtgatcga agtccgttca acagatggag gtgacgaaga cagtctgatt tattagacct
      661 tcacacacca tgagtaaatt acaaaaaatg atttatattt cgctacttgt tgcacaagga
      721 gtgatcattg gtttattgga aaatatgatc ccttatcctt tttcttttgc gcctggtgca
      781 aaactaggct tagccaatct aatcacgatc gttgccattt ttaccatgaa aaaacaagac
      841 agcttcttac tactttggct acgactattc ctgactactt tgctaggcgg aacgatttcc
      901 acattccttt atagtatgag tggtgctttg cttagttatt ttggaatgtt attggtcaag
      961 caattaggac caaaacgtgt cagtatcatc ggaattagtg cggccggcgg tttcatgcat
     1021 aatgtcggac agcttttgat ggcttctttc attgcacaat catggacagt catgttgtac
     1081 ttgccagtcc tttcagtttt agggatttta tcagggattg caatcgggat cgcagcgaat
     1141 tatcttttac gacatgtgca gacacttcaa cgattccaaa aagattatga gcagggaacc
     1201 aaacaaaaat ggagcccaat gtttaccaaa aaataatcgt cacacaaggg gtttttttat
     1261 gaagctacat ccaatgtggg aaaactatcc tgctttaagt aaagaattga cagaaacact
     1321 aaaacggatg gaagaaactg tccgcctgaa aaacaaacct gtcgaacaag ccatcaaaga
     1381 aacgatccat gccggcggaa agctgttgcg tcctgcctat cagctattgt tctcacaatt
     1441 tggaccagag caagatcgag acaaagccat cgctttggca gcttctatcg aaatgctcca
     1501 tacggccaca ctgatccatg atgatatcgt tgatgaagcg gatctgcgac gtggtcagcc
     1561 taatatccgt agtcagtttg gtaatgtcgt ttctgtctat gcaggagact atttatttgt
     1621 ctgctgtttt aaactattgt ctgattactc cacttccttg aaaagtctcc aattgaattc
     1681 tcgcagtatg gaaaaagtat tgaatggcga attagggcag atggatgatc gctataatta
     1741 tgaactttct gtaaaagaat atttggaaaa tatttcaggt aagacagctg aactgttcca
     1801 attaagttgt tctgttggcg cttttgaaag tggaacaagc gagcgcttcg caaaaaaagc
     1861 aggagatatc ggactttcta tcgggatggc ctttcagatt atcgatgata ttttagatta
     1921 tacaaaagag agccaggaaa tcggcaaacc tgtactagaa gacatgcgtc aaggggtcta
     1981 ttctttaccg ttgatctact ctttgcaaaa aaacaaacca aagctactac cttatttaga
     2041 gaagaaagcg gcaatgacag aagaagatgt cgacgccgta cgcaaaatcg ttgaacatac
     2101 aggaggcgtg gaagaagcta ggaagttagc ggcctcttac acagaaaagg ctttgaagga
     2161 aataaagaaa ttacctgcca cctctcttcg aacaaaagag aatctctttt cccttaccca
     2221 actgattttg gatcgcaaag actgaatagg gaaaggttcg atacgtaaat aaatttctca
     2281 gaaggaaatc tacgtgtttt ttgacaaatt gaattgtagt tcttgtattt atgataaaaa
     2341 gcaaaaataa agagcaacgt ccgttagcga cgttgctctt ttttatcttg aatatttttt
     2401 taccttttcg atttcttata actcataaaa gtgattctta aaccgattac aagtaatata
     2461 acgcctgcga gtgttatcca attattcgat tgacttccag ttttaggaag tgctcttttt
     2521 gatttgtttc cattgtcatt gcttgtttta ttagttaagt taaagttcgt cctgttatcc
     2581 gaacttgttt ttgagaagga gacacttgca tgacgtgtat cgcttgtggt agacgacgtg
     2641 tctgtactat cgcttgaagt tgatgaatct gagctatcac ttgtggtaga ggatgtatct
     2701 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg tagtggtaga tgaatctgag
     2761 ctatcacttg tggtagaggt agtatctgta ctgtcacttg aagctgacgt gtccgtgcta
     2821 tcacttgttg tggtagatgt atccgtactg tcgcttgaag ctgacgtgtc cgtgct
//
LOCUS       DACEMO010000231         2865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEMO010000231 DACEMO010000000
VERSION     DACEMO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.238"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            226..897
                     /locus_tag="IW728_002528"
     CDS             226..897
                     /locus_tag="IW728_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1025858.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKINNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKSI"
     gene            complement(1600..1955)
                     /locus_tag="IW728_002529"
                     /pseudo
     CDS             complement(1600..1955)
                     /locus_tag="IW728_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2139..2546)
                     /locus_tag="IW728_002530"
     CDS             complement(2139..2546)
                     /locus_tag="IW728_002530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025859.1"
                     /translation="MSRFFKFFSLLGVMLIGLSTLVGAGTSVYAAEKVNEVPDIETQR
                     QQAVESLKFYLEEAGYVDTETHQYIVTDLEAIQKKAELDGSEGIAGKFILENFVQPTG
                     KRQKRDLQKYAACVVMNSLPFGVLFGIYLIVKI"
BASE COUNT         1008 a          471 c          352 g         1034 t
ORIGIN      
        1 atggaataaa cttgcttttt ttctaccata tcagaattat tgtatttttt attcagaaaa
       61 ataaaatact ccttcttttt ggactgattt tatcgaaact tatttttttc tttttttcat
      121 gtattctttt atttttataa ctagtgtatt acttcaaaat ttgatattat atggcatgaa
      181 cagaattaaa atatcagcta aaataaaagt taaggagcat tgagtatgac aattaaaact
      241 agtctccaaa atcaagctta ccaatctatt cgccgacaaa ttatctatgc tgacttagaa
      301 cctggaaaaa aagtatcaga aaaaaaatta gaagaaacac tttctattgg tcgtacacca
      361 atacgtgaag cactgattca actccgacaa caaggtcttg ttgatacaat tcctcaaagc
      421 ggaaattata tctcattaat tgatttagat tcggccaaaa atgctcgatt tgtcagagaa
      481 catttggaac gacaaattat gttagagtgt tgtgccaaaa ttaataacca aaaaaaacgg
      541 attttagaaa ctataatttc agaacaagaa aaagctgtta gtcaaaaaaa tgctcgtagt
      601 ttttttcata atgataattt attccataaa gcttgttttg aaattgcaga gcgagaagaa
      661 gtatggcaat ggttagaaaa taacaataca cacttagaac gatttcgttg gctacgagta
      721 aaaacggaag gattaaagtg ggaaaaaatt atggaacaac attatcaact atttgaggca
      781 ttaacaaaac aagatccaga agaagctaac tttctaacat cgttgcatct tcatttaatg
      841 cttaatgagc aaaatattgt tttagctact catccaaaat atttcaaaag tatataactt
      901 ttaactcttt ttaaattaaa tgattgttct gatcagaaaa atatcgcttg ttttcttatt
      961 atgtaatata tttcataaac tattctgcat aacaactatt gtttgtactt tttacattgg
     1021 ataactaatt atgactgctg ataacttttg atattaaact agtatctcca atccaaataa
     1081 aaaatagata tcttgttcat caaaattagt tcatggatac ttaccttcat ttacatcatt
     1141 cagcggtttc tcattttctt ttattctatt atttcatttt ctaatagact tggcttcatt
     1201 tcgcaatact tttttacaat aggcatcaaa ttggtgttgt ttagatattt agttgtagtt
     1261 ttgtgacatg ataagtatgc tcctttttgt cttttccact aatacaacga tcaaagggtg
     1321 cttttttcaa aaaactagaa aaatcaaaat aagattcgta ttttgattct atatatcctg
     1381 tttcaactac atcttatatt tcataataga ataaatagcc ttttgcatag ctgcttaact
     1441 tataagaaat tgcaatttac ttcttcaaag tgaaaacgaa tatgagtaaa ataaaattat
     1501 tcttcttaca gataattaaa aatagcgcgt aacaaaaatc ggcattcaat cacataatta
     1561 ggattgatgc cgattactta tattctacgt atagttttag tattcttttt ttatcaccag
     1621 taaccaattg agaacagtcc taaagcagaa tagtctttta tctcaaaacg cttaatttca
     1681 ggcttaggaa tatgaataat cttttgagta tattctttct cttttaatct tactgaagaa
     1741 ataccactgc ctatcttttt tacaaatatt tcatttggtg tacagactaa taaaacaata
     1801 tttgtaatca atccttgcac taaagaatta aaaggaagac tatttaaaac agggtgtgca
     1861 taactataac atgtgaagta gttattttga actttaactt ttaaaaactt taaaaataat
     1921 cttatttgtt cttctaaaca atcatctata ctcatataat cactccacta attacttccc
     1981 ccaacaagaa atagcattaa tagctatacc agctgcaata gttgcagcat tgaattttgc
     2041 aaattgtgca ggagttaaac ttctttttgc tacatctaaa agtattttaa cagcataatc
     2101 ataatttttc ccttgcaacg cattaattaa agtttgcatt agattttcac gattaagtat
     2161 atcccaaaca acacaccaaa tggtaatgag ttcataacaa cacatgcagc atacttttga
     2221 agatcacgtt tttgacgctt tcctgttggc tgcacaaaat tttctaaaat aaatttccct
     2281 gctatacctt cagagccatc taattcagct tttttctgta tagcttctaa atcagtaact
     2341 atatattgat gtgtttctgt atcaacataa ccagcttctt ctaaatagaa tttcaaactc
     2401 tctactgcct gttgtctttg tgtttctata tcaggaactt cattcacttt ttcagcagca
     2461 taaacactgg ttccggcacc gactaacgta cttaatccaa ttaacattac acctaataat
     2521 gaaaaaaact taaaaaaacg actcattttt atccctcctg aaaagtattg ttgattatca
     2581 atttttatcc ttttaagctg ttcaataaga cgtttttacg ttccttccgt ttctatttta
     2641 ttctttctgt cttataacag cttttttcaa gtaaagaacg atataattct tcttaaaaaa
     2701 cagtctattt tttcggtata taataaaatt gtattgttac ctacatagcg aaacagtacc
     2761 aacatcattc aaatgaagat agcaaatttg cttcttcctt tttcttaagt gactatttaa
     2821 ataaccagaa aaaaactaaa atcacactga tatcccaaat aaatt
//
LOCUS       DACEMO010000232         2860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEMO010000232 DACEMO010000000
VERSION     DACEMO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.239"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..1223)
                     /locus_tag="IW728_002531"
     CDS             complement(3..1223)
                     /locus_tag="IW728_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1025860.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1243..1848)
                     /locus_tag="IW728_002532"
     CDS             complement(1243..1848)
                     /locus_tag="IW728_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1025861.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1897..2829)
                     /locus_tag="IW728_002533"
     CDS             complement(1897..2829)
                     /locus_tag="IW728_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1025862.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          782 a          605 c          502 g          971 t
ORIGIN      
        1 ttttatttta cagaagcact cgaaactgca tctactgcag tttcatcgtg tgtgctggcg
       61 ctggttactg catctgtttt tccaccatga acagatgcac ttgaaaccgc atcttcattt
      121 ccaacaagtt gttgacccgt ttgttgtaaa taccactcaa tgattggttt gaaatcaaga
      181 atgtattcat tgattccagc atactctcca ttttcatcat gcattgcttg gtaattatgc
      241 acaatatatt tttctggcac ttgttttggt acatgaacac gtaccgcatc tgttgctccg
      301 cttcgcagtt gttgaattac ccactctaca tttttatacg atttttctgg gtgacagttg
      361 gctaatggat tccctacctg accgggcaca cgagcagcta acatctcttt cgcttcttgg
      421 cgcaaattat aatataaaaa ttggttatta ttatctgcaa aagttaattc catcggcatc
      481 gaatctaaaa agtaattgat ttgatccact gtcaataacc cacgatttaa tttgacgtaa
      541 gtatttccag aaacggcacc gactgcttca gaagcttttt ccaaccaatg atcatctgac
      601 atatcaacat cgggtattgt cgtagcaatt gttcctgtcg catgcaaatc ttctggtgga
      661 agctttttag gccctgctaa ggtagacacg actttaatga attttacaaa acttgctaat
      721 gtactgccta aaaagtcaac agttcgtgga tcaatcaagc gatcttcttg gtcaaatgct
      781 tcttttgctt ttcccaataa aaattcattt ccgggcatca ctaatgcatt cacgcctggt
      841 gcgtccaaaa tttggcgcaa gtgtaattgc gcgcgtgaag ttccttgatc gtagtaagaa
      901 gcacccacaa ccattacagg tttattttca agtggatgga ttttaaatga tagccattca
      961 agtgcacttt tcaatcctgc tggaaccgta tgattgtgtt ctggcgtagc aataattacc
     1021 ccatcagcca ttaagatttt gcgatttagc tgttgaatag caggactatt tgtttgatca
     1081 tcatcttgat tgaacagagg taaatctttg atttcaatga tttctaaatt aaatagttca
     1141 tgatattgtt tttggataaa tgttaataat ttacggttat atgaaagttc agctgctgat
     1201 ccgacgatac ctattaattt catctttgtt tgctcccctc gattaaatcg tttcccatga
     1261 aaagctttcg gcttcacgtt tatttgcttg gtgcgcatgt tgcaattgac tagtgattgt
     1321 gacaaagagt aagaaatctt tgaatagttc acgtaatttt gcgacttgtt cttcatcaat
     1381 gagtcgtcct tgctcatcaa aggcttggag tgaatgaccc aataaaaatt ctgagcttgg
     1441 cataattcgt gcctttaact ctggtgaatc caatatttgg cgcaaatgtg cttgtgcgcg
     1501 tgaagaacct aacgtgccat aagacgctcc ggtaatcatc actggtttat ccacaaacgg
     1561 aaaaatacca tatgaaagcc aatttaatgc gttaatcaat gaagcaggaa ccgcatggtc
     1621 atactctggt gtgctgatga ttacgccatc tgcttgttca atttttccag caatttcttt
     1681 aacaacattt ggtagttctg tgttttcagg tttgttaaat agtggcagct ccttgatttc
     1741 gacaatttcg atttctgcct catttacaaa ataatttttc ataaactgta ataattcacg
     1801 gtttgttgac cgcgttgaat ttgttcctac taatccaata aatttcatct tcattctctt
     1861 tcctttctaa catcgatttc tgactgttcg cacgtactaa attttttcaa atccttttgt
     1921 atacaagaat tgattctctt ttgttacaac tgctccctcg atacccggct gttcttcaag
     1981 cgctttcaac acttctaaag aagatgctcc aaataaacgt gtagtccaga tttctccatc
     2041 tacagaagaa tcagacaaaa tcgtcaggct tgctacgtca tttttaactg gaaagccagt
     2101 tttcggatca atgatgtgat gatacactga cgaaccattt tgtagataac gttcgtagat
     2161 tccagaggta actaaagatt ggttgcttag tttaaccacc gcaagcgact cttcacgtga
     2221 acctttagga tttcgcaaac caatacgcca agcaccatcc gaacgtcttg gtgattcacc
     2281 aaaaacaacg atatttcctc ctaagttaac taaagcaaac gctgcatttc gctctcggca
     2341 atgtgtaatt aatttatctg caatgtaacc ttttgctaac gcccccaagt caatcatcat
     2401 tcctggtttg gttaaaaaaa cggttttcat tttttcatct aattgaattt ggtgtggatc
     2461 cgttagcaga agtaactcag ttatctctgc ttttgttgga accaatgcat cttcaaatcc
     2521 aatgtgccat ttttgtacaa ccggaccaat agcaatattc agataactat ttcttgcaca
     2581 actatgtgct tttcctaatt tgatgagctc aaaaagttca ggatgtacaa tcacagcttg
     2641 tcgtccagca gcttgattga tggccattaa ttcagaagtt gcatcatgtg cgctgaagcg
     2701 atgttcataa acgtgcaacc attcgcctaa ctctgctagt aatcgactag gctcttcatg
     2761 cccaacttgt aatgtaattg ttgtccccat catttgccag attttcgttt cttcgatcac
     2821 ctgattcatt ttgtaccttc tttacttgaa ctacggatta
//
LOCUS       DACEMO010000233         2851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEMO010000233 DACEMO010000000
VERSION     DACEMO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.240"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..820
                     /locus_tag="IW728_002534"
     CDS             131..820
                     /locus_tag="IW728_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1025863.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            834..2336
                     /locus_tag="IW728_002535"
     CDS             834..2336
                     /locus_tag="IW728_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1025864.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQQWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2349..2825
                     /locus_tag="IW728_002536"
     CDS             2349..2825
                     /locus_tag="IW728_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1025865.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT          929 a          387 c          561 g          974 t
ORIGIN      
        1 cattatactc caaaataata aaaaaaactc ctaaatactc catttttatc tttactttta
       61 tgaaagcgga tgctatattg tttttggagt tgttatccaa aaataataaa aaattggagt
      121 gtttaggata atggataaaa agaggttttt agatcttatt tcaaaaggtt tgattgtttc
      181 ctgtcaagca ttacctggtg aaccatttta caaagaggaa gaaagtttaa tgccttattt
      241 tgctttggct gctcaaaggg ctggagcagt tggtatacgt gcaaattctg ttcgagatat
      301 tattgaaatt agaaagtgtg tatcattacc aatgattgga attattaaaa gagattatcc
      361 accagaatct ccattcatta ctgccactat gagagaagta gatgaattag tagaaaccgg
      421 tgttgaggta atcgctcttg attgcactct tagaaatcga catgatggac tatctataaa
      481 ttcttttatt actaaaataa aagaaaaata tcctcatcag ttatttatgg cagatatttc
      541 tacatttgaa gaaggcaaaa atgcatataa agcaggaata gattttgtag gaactacttt
      601 aagtggatat actgaggaaa gtaaaaaaca agatgggcca gatatagatt tgattaaaaa
      661 attggtagaa gaagggattt gtgtaattgc tgagggaagg attcatactc cagaacaagc
      721 aaaagagata aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta ttactcgacc
      781 acaggaaatt gcatcaagat ttgttaaagc attaagttag ggggagaaat aaaatgttta
      841 aaaaattttc acaattagga cgcgctttca tgttaccaat tgctatatta ccagtagccg
      901 gattactatt gggactggga ggagcgttaa caaatgagag tgctataaat gcttatccat
      961 ttttagatca acagtggttg catacaattt taagtattat gagttatgct ggaaatgcag
     1021 tttttgcaaa cctagcacta atttttgcaa ttggtattgc tgtaggactt gcaaatggag
     1081 ataaaggaac agctggttta gctggtggag tagcttatct agtatatact gcaactatta
     1141 gtgggtttct tgaacttttt tcagcaaaag atgcaacact agatacaggt gtagtgggat
     1201 caattgttgt tggtggtact gttgcatttt tacacaatcg ttatagaaaa attgaattac
     1261 cacaattctt aggttttttt ggaggatcaa gatttattcc gattgtttct agtttagctg
     1321 caatcattat tggttctttc ttttatttaa tttggccacc aattcaaaat ggtttagtag
     1381 ttgctggtga acaaattgcc caaatgggta gtttaggaac ttttttatat ggttttttat
     1441 tgcgtttaac aggtgctgtt ggactacacc acaccattta ccctttgttc tggtatacat
     1501 ctttaggtgg taccgaaact gtagctggat caacaattgc tggagcccaa aatattttct
     1561 ttgcccaact agctgatcca aaccacactg gtctgtttac ttatggtact agattttttg
     1621 caggaagatt tgcaacaatg atgtttggat taccagcagc atgttatgca atgtatcgtg
     1681 caattcctaa gaaaaatcgt aaaaagaatg gtgggcttta ttttagcggg gcccttacat
     1741 catttttaac tggaattact gaaccagttg aatatatgtt tttatttgtt gctccttggc
     1801 tttatgtgat tcatgcattc ttagatggat tatcttttta ttttgctgat atcttaaata
     1861 ttcgtgtagg gaattccttt tcaggtggtt tgatcgactt tttattattc ggtgtacttc
     1921 aagggaatga caaaactaat tggttaaaat taattccttt tggtattgca tgggcattta
     1981 tatatttctt tgtttttaca ttctttatca aaaaatttaa ggtagctatt cctggaatgg
     2041 aagaagaatc agaagttcat tctgaaaaag taataaacaa aaatacatca ttacatgaag
     2101 agtctcttat cattattgaa gctcttggtg gaaaagaaaa tattgagtct gttgaagctt
     2161 gtgcaactcg tttgcgtgtt gctgttacga atggggagat tattcaaaaa caaacaataa
     2221 aagacactgg tgcaactgct gtcttcgaag tgaagggtgg catccaagct gtttttggag
     2281 ggaaagctga cttattaagt caagaaatca atcaaatatt aggaaaagat aattaggagg
     2341 tttttaatat gtttgaaatt ttcaaaaaga aaaaacaaga aactttttat gcaccttgtt
     2401 ccggaaaaat catttccatc acagaagtaa atgatcctgt cttttcacaa aaattactag
     2461 gtgatggttt tgccgtcata cctgatggtt caaaaattta tgctccattg gctggtcgag
     2521 taacaaatat atttcctact aaacatgcta ttaatataaa aagcaactca ggcattgatt
     2581 atttgattca tattggaatt gatacagttg aattacaagg agagcctttt tcgatttgtg
     2641 ttcaagaaaa tgatgaggtt actcctgaaa cctcgttggt tgatgtggat tttgacaaaa
     2701 taataaaatc tggtaaagat acttctgtta tagttgtttt tccggagaaa aatcagttca
     2761 atgatttaat aatagataat ggtcgaattg ctcatgggaa ttcatgtgga aaaatcactt
     2821 tttaaaaaag tgtatataaa aagctataaa a
//
LOCUS       DACEMO010000234         2836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEMO010000234 DACEMO010000000
VERSION     DACEMO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.241"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(207..533)
                     /locus_tag="IW728_002537"
     CDS             complement(207..533)
                     /locus_tag="IW728_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025866.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(545..745)
                     /locus_tag="IW728_002538"
     CDS             complement(545..745)
                     /locus_tag="IW728_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1025867.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            820..1470
                     /locus_tag="IW728_002539"
     CDS             820..1470
                     /locus_tag="IW728_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025868.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1525..2691
                     /locus_tag="IW728_002540"
     CDS             1525..2691
                     /locus_tag="IW728_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1025869.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT          999 a          484 c          413 g          940 t
ORIGIN      
        1 agaattagaa tagccttgct tttcaactaa gctttcaact tctttttcat tcattgtcac
       61 acctgcttct gattttattt gctataatag attcaaataa agttttctca gaactttatt
      121 attgagtgtt cccccgatct gcaaactgac gggaacactc tttttaattt tttctacttc
      181 atcaaattta aaaaacgagg tgttttttaa atttgatgac tcaatatcca ttttttgcat
      241 tcttcttttg aaaacaactc cattttttct aataaaataa ctggcaatcc cattctttta
      301 aatttcaaaa aagttgtata tgatacattc aaatatttag ccatctcttt tccttttaga
      361 aatggtcttt ctaatccggt atcaattctt gcttgatcaa tagcctcttt tgtcatttca
      421 aaaacataat ttttcaattg attcgtttgt tgtttatcta acattactgt aattcctgca
      481 tccattattt ttctcctctc attttctatt tcttcaatca gccaactcgc caagttgtat
      541 ttatttagat tttcaaatca ttaagtaacc aatttaccaa cttttgataa gtagatgctc
      601 taatactttc tttgctccca ttttcgattt tgacatatgt tttccgattt atacctatca
      661 attttgctgc atcgttttta gtaagtataa gatcggctct atgtcttctt aagcttctaa
      721 tgaattgatc attaagagtt atcattcagc gcctcctttc aaattattca ctttgtggtt
      781 tttatcttaa taagcacatt ttaaaccaca aaacgagtaa tatcaattaa aatattactc
      841 aatttgtggt tttcatgcta taatagtttg gaatttacta tagggggcgt tactttgaat
      901 agaataaaat tttatattga ttccagtaaa aagaccatta aacaaattag tacagaaact
      961 ggtattaact atacaactct tacgaactat taccgtggtg agcgaaatcc tagaaatact
     1021 gaaacatgga atatccttgc tgattatttc catgttcctg taagttattt gatgggatta
     1081 gacgaaacaa attccataga acaacctaat cttgatagaa taaaaactgc tcgtttagaa
     1141 aaaaaggaca aaatagtcaa gaatattgcc agtcttgact cagattattt tactgaatta
     1201 tctaatctat acttttccat gacttcttta cttgaagtca aacttcaaga agatgactcc
     1261 gactttcttt ttttaaaaag tattctagag gactcctttg aatacacgat tctatttgcc
     1321 aaacaaaaga agtttattaa taaagcttta aacttaaata tcgatgaaag aaaagagtta
     1381 gaatctgagt tatttggcaa cttaacccaa gtacaggtta acaatctaaa aagagagata
     1441 acagctcgat taatatcaaa acttcaataa aactaattat tcaatcagcc aactcgccaa
     1501 gttatcagat tggagagatt agttatgtca atacaaaagt acacaacaaa aagcggtgca
     1561 cgatatagtg tccgcttgta tgttcgtaaa gatgaaaatg gaaagcaaga atactatgta
     1621 aaaaaagggt ttaaaactga aaaagaagcc aagttacacg aagcaagaaa aaaggtcgag
     1681 attgaagata ccggctatca taagccttct aatgacttgt ttataacagt gtatgaagaa
     1741 tggttgcctt cttataggaa tcgtgtacaa gaaacgacat atcaacgtac aaaagacctt
     1801 ttccgcttgc atatactacc aaaattcggg aataagtcca tttctaagat tactcctgtt
     1861 cactgtcaga atgcagtcaa tgaatgggct ttaaaattca agaatatcaa acagcttaag
     1921 tcttacagct ctcaaatatt tgagtatgcc atttttgcag agctattaca ccgaaatcct
     1981 atggcaaata caaatctacc aaagcgggaa aagaaagaaa atgaaaatta cttttcatta
     2041 gaagaactaa aagctttttg ggatattctc aataaagaag agcctttaaa acatatttta
     2101 atcttccagc ttttgattac tactggtata cgtaaaggag agctagctgc tcttcattgg
     2161 tctgacattt attttgaaga acagctaatg tatgttagaa agagctatgc aactattcga
     2221 aatgaagaga agaatgcaag aagaaaaact gtcaggattc aaaaaaatac aaaaaatata
     2281 tcttccgaac gaatattacc aattgatgca caaactattg aaatgttaaa aatctggaaa
     2341 aaagaacaag ctcgagagct tttgcagttt ggcataaata caaatacaaa aaatcaactt
     2401 atctttacct atgtaaatgc agacggtgac attaatcaac ctttgcatgc ggactattca
     2461 aataatatat tgaaacgttt agagaagaaa tataaattta agcacgttac tattcatgga
     2521 ttccggcaca ctcacgctac tcttctacta gaaggtggag caagtatcaa agaaacacaa
     2581 gatcgattgg gtcataaaaa tgctgaaact acccttaaca cttattctca tgttactgaa
     2641 aaagctcaac gaaatgcgtt agaaaaattc attgcttaca ctggtttata atattgtcat
     2701 tacctttctc attaccagaa cgaaaaaaag acaccttctc gcaatgagaa agtgtctgaa
     2761 atcgttggta taacaacgat taacgttttg aaaactgtga agctttacgt gctttcttaa
     2821 gacctggttt tttctt
//
LOCUS       DACEMO010000235         2815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEMO010000235 DACEMO010000000
VERSION     DACEMO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.242"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            807..1007
                     /locus_tag="IW728_002541"
                     /pseudo
     CDS             807..1007
                     /locus_tag="IW728_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1494..1673
                     /locus_tag="IW728_002542"
     CDS             1494..1673
                     /locus_tag="IW728_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025870.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1719..2282)
                     /locus_tag="IW728_002543"
     CDS             complement(1719..2282)
                     /locus_tag="IW728_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1025871.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDIQRPEFQKLLNYIREGDC
                     VIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQKRLVFKTVVDMLNQ
                     GVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            complement(2310..>2815)
                     /locus_tag="IW728_002544"
     CDS             complement(2310..>2815)
                     /locus_tag="IW728_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1025872.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIK
                     VLMGITA"
BASE COUNT          890 a          490 c          517 g          918 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atttaaaaag aaaagtgaca
      361 gataggaata aaaacttatt aaatgtagtt atgatctgtt atatttcatt tctaatacta
      421 ggtcttccac aagcagtgtt tcctgaaatg ttttctggag actggggtat tgtcagctat
      481 tctatattaa cattgattgc tatggtttta ggtacttcat tttataatgc ttttgtaagt
      541 aagcatgttg aggaatatga agtattaatg tacttaaaag agaaaaaaac aagtaattaa
      601 tatgagaaca ccacgcatag tttttatttt gagatattct agaatgaaac acattaagaa
      661 cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc taagtttttt catatttgtt
      721 tgttatgcta tacatagatc tgcataatta gacttgcgct gcccgttatt gaagtttcag
      781 gcagcgctct ttttggggga tcgataatgg aagaagaaaa aatatttgag aaacgctggc
      841 agctagcatc aagtgaacaa agagcaagat acaataattt gatcacctcc taccccactg
      901 ttgactagac atataaggaa aagaaatact tactttggct gtgccagtta gatatagata
      961 cctttgaaac atttgaagtg atcctagata aaattaaaca gaattgatca gaaacaagcg
     1021 aagccttctc tcggttcgct tgttttgcta tttattagtt ggctcagggc aactctaagc
     1081 tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta ccactttaga atacatggat
     1141 gtacgtattg ccattgactc ctattagtta ctgaaaaaaa atttaaacaa gttgagtatt
     1201 gcagataaat agctccagtg aggtgaataa ctgctccact caaggcaata gatagctcca
     1261 tccagggcaa caaactgctc cactttcata tttaatagat gaataacctt ctgtataata
     1321 gaaaggcata cctttcggta tgacaactta tacagaatgt tattttattg gttcactatc
     1381 gaaaaatttt ggagctttat ttttccggta tttctcaacg aacgattagt ttaactgtgg
     1441 gaagctccag acatacgatt cgagaagtta cttctcgagc agacaggaag ggggtgactg
     1501 aactaaccgc tgatatgagc gatacttggc tgcaggaata tctttttccg gaaaaacaaa
     1561 caacagcacg aggttacctt cctgtagact gggaatatgt tcataaagaa ctaatgaaaa
     1621 agaatatgac attgaaactc cttcatagag aatatggggt acatggaaca tagaaacgaa
     1681 aatgcacggt agaaaagcga atgaatatag tataaattct acaaatcatt cgctttcttt
     1741 attttatata tggttggtcg agagagacca ttttctttgg caatttctgc aactggaact
     1801 ccttgattta acatgtccac cacagtttta aagactaatc gtttttgcgg atctgttgag
     1861 ttctcagaat attcaattgg tcgcccctta tatcgaccag cttttttggc caataatact
     1921 ccttgccgct gacgttcacg gattttctca cgttcgtttt gtgcaatata acttagcaat
     1981 gataaaaaca tatcgaacat agcagtatct aggagttcat ttccagtatt aaaatctaaa
     2041 aactgtgcat ctagtatttt taatgctact tttttctgtt tcataaaggt aactgtattt
     2101 tttatgtctt cataatctct gcctaggcga tccagtgatg caacaatgac acaatcacct
     2161 tctcggatat aatttagtaa tttttgaaac tcaggtcgct ggatatcttt accactggct
     2221 ttttcttgga ataatttatc aatgttaagt ccttcggttc tgttgcgaag tttgaaaatc
     2281 aaacgcgccc tctctgaact ccaaatatct taggctgtta ttcccattaa taccttgatt
     2341 tcagtagaca ccgaaaagcc gaagagcgtt ccatttcttc ggttcttttt atatattcct
     2401 cgaatggtct ccatgccctt aatcgtggaa gaggctgtac ggagactttg ataaaattta
     2461 tttcgtcgtt taataggtcg atggtcttgt tctattaaat tgttaagata cttcacagtt
     2521 cggtgctctg tcttagtata taaacccaca ctctgtaact ttctaaaggc ggagccaaga
     2581 gaaggtgctt tatcggtcac aattgctttc ggctcaccaa actgtttatg gagtcgtttt
     2641 aagaaagcat aggctgcttg cgtatcccgt ttctttcgta accagatatc taaggttaag
     2701 ccgtccgcat caattgcacg ataaagataa tgccaacgtc ccttaatttt gatataggtt
     2761 tcgtccattt tccatgaata gaaggattgt ctatttttct tcttccaaag ataat
//
LOCUS       DACEMO010000236         2778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.243, whole genome shotgun sequence.
ACCESSION   DACEMO010000236 DACEMO010000000
VERSION     DACEMO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.243"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..133)
                     /locus_tag="IW728_002545"
     CDS             complement(<1..133)
                     /locus_tag="IW728_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1025873.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     "
     gene            455..1108
                     /locus_tag="IW728_002546"
     CDS             455..1108
                     /locus_tag="IW728_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1025874.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            1193..2098
                     /locus_tag="IW728_002547"
     CDS             1193..2098
                     /locus_tag="IW728_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1025875.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(2100..2732)
                     /locus_tag="IW728_002548"
     CDS             complement(2100..2732)
                     /locus_tag="IW728_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1025876.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
BASE COUNT          894 a          493 c          550 g          841 t
ORIGIN      
        1 ccagctcttc aaggaatgcg gtgacttttg ttgcatagaa aggaaaggca atcgctggtt
       61 ctccataaac aaccgcgtgc tcgcccatca atatgatctt tccgcttgat tctccttggc
      121 catagtttgc cataatctaa ctccttctca cgtaaaagtg atttttaagc aaaaaataaa
      181 gcgcccaaaa cgagttgcgc tgcttcacga atttttttgt ccactaaggg tctcaaaaaa
      241 cattcctcct ttattttact aaaaccatct cagaagtaaa actaagaata cgttttctaa
      301 agaaaaaaca cttttttctt aagaattttt tgactttttt tcttctttga ctagaaaaaa
      361 atacaattga cactactaga tattgggggt atatttaaaa aggttggagt taaacacaat
      421 atttagtgat gaagtgagct ggaggtaaat gagcgtgatc gttttagcag gtacaattgg
      481 tgccggaaaa tcaagtttaa cagaaatgat ggctgaacac tttgatagtc aggcatttta
      541 tgaatcgatt gatgataatg aagtcttgcc cttattttat gcaaaccctg aacagtatgc
      601 ttttttgctg caaatttatt ttttgaataa acgttttgca agcatcaaac aagcaatgaa
      661 agatgacaat aatgtattgg accgttcgat ttatgaagat tcactgcttt tccatctaaa
      721 tgcagatcta ggaagagcaa cagaaacaga agttcgtgtg tatgatgaac tgctggaaaa
      781 catgatggaa gaacttccct atgctgccca taaaaaacat ccggatttat tagttcatat
      841 tcgtgtatct tttgatacga tgttggagcg tatcgaaaaa cgtggtcgtt cgtatgaaca
      901 attgtccttt gatccaagtc tatacgatta ttacaaagaa ttgaacagac gttacgatca
      961 atggtatgaa gaatacaaag aaagtccaaa aatccaaatc gatggagacc gctataattt
     1021 tgtggaagat cctcaagcaa aagaagaagt cttgaaaatg atcgaagaaa aattagcaga
     1081 gattcgccaa accaaagtag ccatttgatt tacagttgct caaataaagg gtaaaccttt
     1141 atttgagcga cttttttctt tatactggaa acaaatgtca ggaggataga gtatgcggga
     1201 aaaacactat tttaatcaag accgtaattt ttggatcaat gtacagcaaa acgatgtgct
     1261 agctgttaaa aacttaaaag aagaatatgg cattagtgac gagatgctga cttattctct
     1321 agataaaaat gaacgggcgc gcgtagagta cgattctttt gatgaagcgt tactgcttgt
     1381 atcaaatgtt ccgcaccagc aaaaagtcga taatcattat gaaactagtc ctatcgcttt
     1441 tattttaaaa gaagacggtt tgtttacatt taccacgccg aataccgagt acgtgattcg
     1501 actaattcga tcgcttttgg aacgaatgcc agacatgagt gtatacagtt tattgtttcg
     1561 tacattgttt ttgatttctg attccttttt tcctttaatc gaggaagtga atagtgaacg
     1621 tcagcggctt aatctgaaat taagagaaaa aacaacgaac aagaatctgc tgcaactttc
     1681 agatttagag atcggtttgg tttacttagt tacaggaaca aaacaaaatg tagtactttt
     1741 agaacaaatc aaggcattgg ctatttatcg caagttgtca gaaaaggaaa aagaagagtt
     1801 ggacgatgcg ctgatcgaag cacgacaagc agttgaaatg acgaacttag cttcgcagat
     1861 tttagaccag ctctctggca cttataataa tttgctgaat aacaacttga atgacacgat
     1921 gaaatttttg acggtctggt cgctgatctt gaccgttcca actattgtga caggattctt
     1981 cggaatgaat ctccagttac cctttaccca ttccgttttc ggatggggaa tcgccttgat
     2041 catcagtttg gtcttatcta tttggatgct tattgcactg tggcggcgga ttcgttgatc
     2101 tagtctttga tctgcaaacc aagggcttgg gcgattacaa gtgcttgctc ttggttgacg
     2161 attagtcccg ccaaagaatc ttgagataaa gcgattgctg tgaagaaatt agtcgtaaag
     2221 tctaatccct tcagagaagt tcgaaaccag ttcgaaccgg tcaactgatt gtcagacaaa
     2281 aaaaggtttt tccaagtgac ctcataaaat tcgctgtctt ctaattgaca atgatcgaag
     2341 gagacagctt tcaagtttgt actactaaag gaagccatcg aagcaataca atcagtgaaa
     2401 gtacagtctc gaagataact ttcggcaaaa ttcgtacctg ttaatttaca ttgatgaaac
     2461 acgacttggt ggaaactgcc accaatccac tccagattgg aaaaatcaca tttttcaaaa
     2521 atgacattac tgcattcaaa atgacgtaaa actgtttttt gcatcgtaag tttttggata
     2581 tgactctctc ttaataccat atttttggca tcgagatagc tttggtcacc cccgttgatc
     2641 agaagaccag agaagatctc ctcatcttct aaataaaact ctccctttgt aagaagagga
     2701 agagaaggtg gaataggttc gatttttttc atgtgggttc acctcgtttt tctttagtat
     2761 agcaaagaag cgggaatc
//
LOCUS       DACEMO010000237         2777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.244, whole genome shotgun sequence.
ACCESSION   DACEMO010000237 DACEMO010000000
VERSION     DACEMO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.244"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(550..2163)
                     /locus_tag="IW728_002549"
     CDS             complement(550..2163)
                     /locus_tag="IW728_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1025877.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2304..>2777)
                     /locus_tag="IW728_002550"
     CDS             complement(2304..>2777)
                     /locus_tag="IW728_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1025878.1"
                     /translation="QMSIHLLSLLVTYCLQKIQLSSLPRHYQQELALLTIPSNEKVIP
                     KKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQIFFISPASLKKHFRKVNGKSVISY
                     FKELKMEKAKRLVLENELTYTEIADILGFHSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT          888 a          508 c          484 g          897 t
ORIGIN      
        1 aaataaatag gaacaaaaaa attaaattat ttaatacaca cattagggat cattcattga
       61 aggattagaa gacaacgagg atggcgaaaa atatgattac aatagaaaag tcgttctaaa
      121 ccaatcagtt cctagtatca cacaacgcaa gccagtcgat ttggtgagat tagaggagtg
      181 aaagcaaact ttattatata agaaaaaata ttcgaaaggg atgctagttt aggtactagt
      241 gctgatagat taatatgatg cctcgtaata ttaggcgtag ttactctaga gacatcacta
      301 tggactatga tttgtctcag agtcacaaca tgttttttta tgaaaagaaa cagctaaaat
      361 aattaaattg cgcaatgttt aaactttctt aaactataac ctaataaatg tgaagaaata
      421 atgcacaatt tcttcacatt tatcttctat tctatataca taccaaccaa caaccctata
      481 tactttttca tttttactcc tcaagtaaaa ataactccta gaccgcggct acccaccgcg
      541 gtcgtttttt tattcttcaa tcaaaatcgc tttgataccc aaacctgcca agattcgggc
      601 agaagtataa gtattcaatc ctcttctgaa agtaatgtaa acttttttat cttttgggat
      661 ttcattgatt cgatctcgta actcattcat tggaatattt tttgttgccg agatactgcc
      721 agaaggtgct ttattaggtt cacgtatatc taaaaagaac gcttctttca aatcgttttc
      781 gggtatatca cttgctttga tcgtttctac gatattcgtc atttgattga ttgcgacata
      841 accagctata ttcaacgggt cacgggtagt agaatacggt ggagagtaag gcaactccaa
      901 atcaggcaaa tcaaagactg tcaagtttcc ggtgattgct acagaaagtt ctcccatccg
      961 tttatcgaca cctttttctc caaatgcttg tccacctaaa atacgaccgc tatctgcatc
     1021 aaaaatcagc tttagattta atcttgtagc ttctggatag aaataggcat ggtcaaaggg
     1081 agtgataaaa acagttttat aatttgttat gcctgatgct tttaattcat gttctgtcat
     1141 tcccgcataa cttgctgtat gatcaaagat ttttgcgaca cctgcaccaa tatatccgcg
     1201 atagcgcata ggcgtaccat ttaaaatatc tgccagcata tgtccttgac gattggccgc
     1261 actggaaagc atactttgga tcggctgacc agtcactaca ctagtcgttt cgataatatc
     1321 gccaatcgca tagatgtccg gcagattggt ttgtaactgg tcatcaacaa tgatctgacc
     1381 tgtatctgat aattgtatgc cggttgcttt caccacttca ttcttcggag aaacaccaac
     1441 agcaaaaatt agcatatcag cagaaaggac ggagccatct gataatacta tttctcgacc
     1501 tttatcacgg atctcagtaa ctcttgtttc taaatgaacg gctaatcctt ctttcagcaa
     1561 tttgtcataa actaaattag caatttctgg atcataaggg aaagcgactt gtggcaattg
     1621 atcgattaaa gtcacgtcta agccgcgatg cttgaaattc tcagctaatt ccagacccat
     1681 aacacctgca ccaagaatga cgacatgttg tggatttttt tcttcaagga acgatttgat
     1741 tcgatccgca tccgtcacac tacggaggac aaagccgttt tctgcttcaa aaactccggg
     1801 aatatccgga taatctggtc tagcaccaga agagatgatc aacttatcgt aatctgtttt
     1861 tatttcttca tttgtggata ggtctttaac tgttaatcgc ttagtagaag gatcgattgc
     1921 tgttacttcg tgtttagtaa agacatcgat gttgtttttt gttttcaata tttctggtgt
     1981 acgttcgatc agcgagtcac ggtcagtgat cacacctcct aaataataag gcaaagcaca
     2041 acttgcataa gaaatatttt ctccgcgttc atagataatg atttcgtgcg cttcattcaa
     2101 tcgacggaaa cgagtggcaa atgaaggacc gccagctaca ccgccaatga caacaacttt
     2161 cataaaaaca tctccttttc tacgttgatc gtactatgtt atggtaaaaa acagcaaata
     2221 attgtgccgg aaaattattg tttattttga atagacagaa aatatagaga aataatttgt
     2281 ggataaaacc taaaaatttt tttctatttt aaggatagat aatactttga aggggataac
     2341 cctgtatatt ttttaaaagc tgaagaaaaa tgatggatcg aatgaaatcc taatatatct
     2401 gcaatttctg tataggttaa ttcattttct agcacgagac gttttgcttt ttccattttt
     2461 agttctttaa aatagctaat aacacttttt ccattcactt ttctaaaatg tttttttaag
     2521 ctggctggac tgataaaaaa aatttgggct aattgttcaa gcaaaaaatt ttctgaaaga
     2581 tgttcttcta aatatcgtat gattggatca actaattcat ctgaataaag aatatgcttt
     2641 tttttaggaa tgactttctc atttgaaggt attgtcaata aagcaagttc ctgttgataa
     2701 tgccttggta aggaagacaa ttgaattttt tgcagacaat aagtaactaa taaggaaagc
     2761 aaatgaatgc tcatctg
//
LOCUS       DACEMO010000238         2722 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.245, whole genome shotgun sequence.
ACCESSION   DACEMO010000238 DACEMO010000000
VERSION     DACEMO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.245"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2397
                     /gene="fss3"
                     /locus_tag="IW728_002551"
     CDS             <1..2397
                     /gene="fss3"
                     /locus_tag="IW728_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1025879.1"
                     /translation="EGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIAT
                     LDEIPHGAKVTITEKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGV
                     ETGTDLWNDKYSLAGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYV
                     TETKASNGFVNTFKPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNET
                     QGKAEFKGAEYTLFTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHL
                     AINEYYWQETKAPEGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFK
                     FAGSAAGTAETGFNDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYG
                     DYLLEEVEAPEGFQKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYE
                     KLTNKAFSVSLNRLMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEI
                     KEDWYVVAQAIDVEATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQV
                     LDKTIVLFNYVYENKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGS
                     QTFTHGDVVDMFDDVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDK
                     EFTKTVLAEKVDTSKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTT
                     PSAPEQPEIPTVPSDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN
                     "
     gene            2511..>2722
                     /locus_tag="IW728_002552"
     CDS             2511..>2722
                     /locus_tag="IW728_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1025880.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQP"
BASE COUNT          987 a          486 c          590 g          659 t
ORIGIN      
        1 gagggttctt tgaagattaa aaaagtcgat aaagaaacag gaaatgttgt accgggaacc
       61 gttttccatt tagactttgg aaaaacatta cctgcaaaag acgtgaccac cgataaagaa
      121 ggcattgcga cattggatga gattcctcat ggcgcgaaag taaccattac tgaaaaatca
      181 gtgccagctc cttatacgat tgacactaca ccaatcaccg caaccattaa atctggtgag
      241 actatttctg tgacttcaaa aaatactcgc gaaaaaggtc aaatcatttt gaataaaatg
      301 ggggtggaaa caggaactga tctttggaac gacaaatact cattagccgg aaatacgttt
      361 gccattcgca aagacagtcc aaccggtgaa attgtccaag aaatgaccac ggatgaaaaa
      421 ggtcatgcgg aaacaccaaa agagattgcc aatgcgttgg aattgggaac ctattatgtg
      481 accgaaacca aagctagtaa tggctttgtg aataccttta aaccagtaaa agtcgaattg
      541 aactatgtca atcaaaccgt ggctcttgtt accagtaacg taaaagggca aaaccaagaa
      601 atcactggtg aaaccacttt gacaaaagaa gacaaagata ccggtaatga aactcaaggt
      661 aaagctgagt ttaaaggagc tgaatatact ctctttactg caaaagatgg tcaagcggtc
      721 aaatggagtg aggcttttaa accagagtta gtgaagggaa cgaaagcttc tgatgaaacg
      781 gtgactttga ctttagatga aaagaatcaa gttgctgtta aacacttagc gattaatgag
      841 tattactggc aagaaaccaa agcacctgaa ggatatacct tggatgaaac aaagtaccct
      901 gtttccatca aaaaggtgga tgataacgaa aaaaatgccg tgattacccg agatgttacg
      961 gcaaaagaac aagtcattcg ctttggcttt gatttcttta aatttgctgg atcagctgct
     1021 gggactgccg aaactggctt caatgatctg acctttaaag tatcgccatt ggaaggaacc
     1081 agtgagatta cagatgccga agatgaagca attacagctt ataacgagca actaggtttt
     1141 gatggctatg gcaagtttga aaatcttcct tatggggatt atttacttga agaagtagag
     1201 gctccagaag gatttcaaaa aattacgcca ttagaaatcc gttctacttt taaagaaaat
     1261 aaagcggatt acactaagag tgaatacatt tttacgatta ccgaaaaagg acaaaaacaa
     1321 ccaattaaaa cggtgaccgt tccttacgag aaactgacga ataaagcatt ttctgttagc
     1381 ttgaatcgtt tgatgctcta tgatttacct gaagaagaag atagcttaac ttctcttggg
     1441 acttggaaag acggaggcaa agaattgaca gaccttgatt ctaccgagct agttgataaa
     1501 ttgagctata acttgcatga aatcaaagaa gactggtatg tggtagcgca agccattgat
     1561 gtggaagcga caaaagctgc ccaagaaaaa gatgaaaaag ccaaaccagt ggtaattgcc
     1621 gaaacaaccg caacgttggc gaataaagag aaaacaggaa cttggaaaat tttgcataaa
     1681 ttaaccgcag aacaagtttt ggataaaacc atcgtcttat tcaactatgt gtatgaaaac
     1741 aagaaaccct atgaagcagg tgatgatcca gtagcgaaag atgctagctt gaacaatcaa
     1801 gcccaaactg tcaattgtac ggtggaacgc catgtttcca tccaaacaaa agcccaccta
     1861 gaagatggtt cgcaaacctt tactcatggg gatgtggtgg atatgttcga tgatgtgtcg
     1921 attacccatg atgtactgga tggctcaaaa gaagcttttg aaacaattct gtatgcatta
     1981 cttccggata atacgaccaa agaaatttgg aaatctggca aaattgatta tgaagtgaat
     2041 gacaaagaat tcactaaaac cgtacttgcg gaaaaagtgg acacaagtaa atatccagaa
     2101 ggtacaagtt ttaccttcaa agaaattaac tatggcaaag acggaaacat caatgggaaa
     2161 cacaatgagg atctaaaaga aaaaactcaa actttaacac ctaaaaaagt tccgacaaca
     2221 ccaagtgcac cagaacaacc ggaaatacca actgttccaa gtgactctca agaatctagt
     2281 cctacagtaa agacattccc acaaacaggg gagaaaaatt ctaatgttct gctgtttatt
     2341 gggtttacct tgatctttgc gactgctggg tattatttct ggaatcgccg aaactaaggt
     2401 gatgtgatga aacaactaaa taggaaaaac caacgtgcgc gaccgcctcc taaaaaaagt
     2461 ggtcgcgcta tttttgtatc caaaactaaa aatgaaaaga ggaaaagaag atggaattga
     2521 aatttgttgt gccagatatg gccgaaacct ttggaaagat aaggtatgcc ggcgaaggcg
     2581 aagttttaac tgaaggatat gggcggaata ctacagtaat tggtcgtagt tatcatttgt
     2641 attccagtaa gcaacgagcc gatgatattg aagtggtggt agctgcggaa gccggcgaaa
     2701 aggattttga tcaagatcaa cc
//
LOCUS       DACEMO010000239         2694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEMO010000239 DACEMO010000000
VERSION     DACEMO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.246"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2421
                     /locus_tag="IW728_002553"
     CDS             <1..2421
                     /locus_tag="IW728_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1025881.1"
                     /translation="SLISYYLGGIFTPLVFFFPNQMMPDALYVLTLLKVGCAGLAFWF
                     LARTFKIPRWGHVALSVSYALISFITAHSEIIMWLDAFFYLPLVILGIHRVMDLKRPK
                     LLFISYLLLFLSSFYMGFMIGVFSFLYFIARLLTNWSEYKKTIVPYGITSLLAGGASM
                     IIVLPAVLDLRSNGEALSQITTLKTEATSYLDLIMKNMIGVYDTTKYGSIPFVYAGLL
                     PLVLCLFYFASRKVPLKNKFLYGSLFAILIASFYLVPLNLFWHGMHAPNMFLFRYAYL
                     FSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAALFAVAWGIKPEGSYTYVTMTSFVLT
                     VVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMSAEALVNTNSMIHGILDDWNYASRSL
                     YTDPYPDLKALVNETKKDSDSFYRLENLDPVSANDSINYGYSGISLFSSIRNRNSSSY
                     LDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYNISKNESTFSKYGFRQAGQSGDYTLY
                     QNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNLINTLSGLNEQYFTFYTPTLKNQLNT
                     TITKTTTGVTYSETADNQPKELTWEVHVPANTQAYLSLFPTNFAQLESSSATITVNGT
                     SRKTQINISGQYYDLGYYPQETTVTFTASFYGTKEISFMEPKVVGLDVVAYQAAMNQI
                     KEKGVEMEATGRKASGTVNTAEDSMLVTTIPYDKGWKAKIDGKPVKVSNFKDAFLMIK
                     VPAGKHTVTFSYLPEGFTIGAILFVLCIALFILYVWYLDRNRPFYGTIDPAIEEQPRN
                     THRRRRRK"
BASE COUNT          772 a          527 c          542 g          853 t
ORIGIN      
        1 tctttaattt cttactatct aggcgggatc ttcacgccac tggtgttctt ttttccaaac
       61 cagatgatgc cagatgcact gtatgttctg actttgctta aggtcggctg tgccggattg
      121 gctttttggt ttcttgcacg tacgttcaag attcctcgct gggggcatgt tgcacttagc
      181 gtcagttacg cattgatctc atttatcact gcacattcgg agatcatcat gtggctggat
      241 gcattttttt atcttccatt agtgattttg gggattcatc gtgttatgga tcttaaacgg
      301 ccgaaacttt tatttatcag ttatttactc ttgtttttat ccagttttta tatgggtttt
      361 atgattggcg tattttcttt cttatatttt atcgcccgtc tattgacaaa ctggtcagaa
      421 tataaaaaaa cgattgttcc ttatggtatc acttctttac tggcaggcgg cgcttcaatg
      481 atcattgtat tgccagcagt gctggatctt cggtcaaatg gcgaggcact gagtcagatc
      541 actacgttga aaacagaagc gacttcttat ctggatctaa tcatgaaaaa tatgattggt
      601 gtttatgaca cgacaaaata cggatcgatt ccgttcgttt acgcaggtct gctaccatta
      661 gtcctctgtc tgttttattt tgccagtcgg aaagtaccgt tgaaaaataa atttttgtac
      721 ggcagtcttt tcgccatact tatcgcaagt ttttatcttg ttccattgaa tttgttctgg
      781 catggtatgc atgcaccgaa tatgttttta ttccgttatg cctatctgtt ctctttctta
      841 gtgctcctgc ttgcaggaaa aggatgggaa aagatagaac aagacgatcg tgggctgttg
      901 ttaggaatca ttttggtcgt agctgctctc tttgcggtgg catggggcat caaacctgaa
      961 ggaagctaca cctatgtgac gatgacttcc tttgtattga cagtggtttt cttagggatt
     1021 tatgcttttg ctgtgggatt ttatcagcat aaaaaacttt cattcaatca tctgagtata
     1081 ttgttattat tgatcatgtc tgctgaagca ctggtcaata cgaacagtat gatccatggc
     1141 attcttgatg attggaatta tgcttcccgc agtttatata ctgatcctta tcctgacttg
     1201 aaagctcttg taaatgaaac aaaaaaagat tccgactcgt tttaccgctt ggaaaatctg
     1261 gatccagtat ctgcaaacga ttcgatcaac tatggttaca gtggtatcag tttgttttct
     1321 tctataagaa atcgtaattc ttcctcttac ctggataaat taggtttccg ttcaagaggg
     1381 acaaatttga atatccgtta taacaataat actttgctga tggatgggtt tacaggaatc
     1441 aaatataaca tttctaaaaa cgaaagcacg ttcagtaaat atggcttccg ccaagctggt
     1501 caaagcggcg actatacact gtatcagaat gtcaatgcat tgccactagc atttactgca
     1561 ccgttgtccg ttaatgatgt tgagcagcca gtgacagata atttgaccag tcagacaaac
     1621 ttgatcaata cattgtctgg tctaaatgaa cagtatttta cattctatac acctactttg
     1681 aagaatcagt tgaatacgac gatcacaaag acaacgactg gcgtcactta ctcagaaaca
     1741 gcagataacc agccaaaaga gctcacttgg gaagtccatg tgcctgcaaa tacacaagct
     1801 tacttaagtt tgttcccaac caattttgcg caattagaaa gttctagtgc gacgatcact
     1861 gtcaatggta catcgcgaaa aacgcaaatc aacatttctg gtcaatacta tgatcttggc
     1921 tattatcctc aagagacaac agtgacattt actgcaagtt tttatgggac gaaagaaata
     1981 agtttcatgg agcctaaagt cgttggtcta gatgttgtag cctatcaagc cgcaatgaat
     2041 cagatcaaag aaaaaggtgt cgaaatggaa gccactggca gaaaagcttc tgggactgta
     2101 aataccgctg aagattccat gttggtgaca actattcctt atgacaaagg atggaaagct
     2161 aaaattgatg ggaaaccagt caaggtcagc aatttcaaag acgcattctt gatgataaaa
     2221 gttccagcag gtaaacatac tgtcactttc agttacttgc ctgaaggatt tacgataggt
     2281 gcaatccttt ttgtcctatg tattgcattg tttattcttt atgtctggta ccttgaccgc
     2341 aatcgcccct tttatggcac gattgatcca gcaatagaag aacaaccacg aaacacacac
     2401 agaagaagac gtagaaaata aagaaacgat ggaacctgtt cagactgtcc gcaccaaggg
     2461 caatcaaaac aacgttccat cgttttttgg ttacagaaaa aataggtaac aaaaagatga
     2521 ttcacttatc aacgcctcgt ccacaagtca cgatattcat caaagatgag aagctgtttt
     2581 ttgaaattgc tctttgtgac tcagagttaa atgacaggct cacgacctct ttcttaaacg
     2641 gtgttcaatc agctgacctt cggtattagc tcaacaaacg gcaaaatatg agga
//
LOCUS       DACEMO010000240         2656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.247, whole genome shotgun sequence.
ACCESSION   DACEMO010000240 DACEMO010000000
VERSION     DACEMO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.247"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            129..455
                     /locus_tag="IW728_002554"
     CDS             129..455
                     /locus_tag="IW728_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025882.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            452..1266
                     /locus_tag="IW728_002555"
                     /pseudo
     CDS             452..1266
                     /locus_tag="IW728_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
     gene            1405..1716
                     /locus_tag="IW728_002556"
     CDS             1405..1716
                     /locus_tag="IW728_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025883.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            1713..1943
                     /locus_tag="IW728_002557"
     CDS             1713..1943
                     /locus_tag="IW728_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025884.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            1950..2153
                     /locus_tag="IW728_002558"
     CDS             1950..2153
                     /locus_tag="IW728_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1025885.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            2150..2449
                     /locus_tag="IW728_002559"
     CDS             2150..2449
                     /locus_tag="IW728_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025886.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
BASE COUNT          980 a          402 c          641 g          633 t
ORIGIN      
        1 attcgaagcc ttcggcgtga gattgacgat ctggaacgaa caaaggcagt gatggtcaat
       61 gaaacggcta gaaaggcaat cgatttgcac atagagaatt taagaaggga aatccgtaga
      121 ttggaggaat gagcgtggat aaggaagcgg cttggcgaaa attaatgttg ctgattcaag
      181 atgagaactg gcaagaagat gaagcagtgg ttgctgaagt tcagcgtcta ggaaagattg
      241 ctaacggacg tatacgaaaa aagccagaca aaagaaaaca gcgcaaaggg aaaatcgtcg
      301 ttgttttaca cgaaggcaaa attttgatgc aaggaacagc tagtgagctg tctgcagaaa
      361 ctggatatac gcgtgggact attcgaacgt acgcttggcg aaatcacacc gatcgaaaag
      421 ggcacgaata taagtatttg gaggaagaaa aatgaacgaa aacaaattaa tcaaattggg
      481 tgtagcagga gcagtaatcg taggtattgg agttatcgga ggatttaagt tcttcgaaaa
      541 aatcgataat ggatatgtgg gtgtgcgcta ttcaatgaac ggcggtatca aagatgaagc
      601 actgacgcaa ggtgtgaaat ttgtagggat tgacaaagtg atccaatatc caattcgctt
      661 gcaaactatc caatcaaaaa atatttcagt atctacaagc gacggcaaaa agacaacgat
      721 tgatatcaaa tatgactaca aagttgattc aactaaagca gcaaaaatgt acaaggaatt
      781 tgggaatatc acttcggaag atatcgaaag tggatggtta aaatctaagc ttcaaaaggt
      841 cgctcgtgaa gtttatgcga aatatagtct gctgatgtcc tttcaggaga ttcctctaaa
      901 gttgaagctg aggtattaac gaactttgct aaatcagttg aatctaaagg gtttgaagtc
      961 gaagacgtaa cacttggtgt tccagatgtc gataaagaaa cacaaaaatc aatcgatgcg
     1021 atcattcgag ctggtcaaga aaatgaaaaa gcgaagctag acgcagaaac tgcaaaaact
     1081 caagctgata gtgaagctta caagaaaaca aaagctgcag aagcagaggc agaatctaat
     1141 cgcaaagtcg ccgaatcagt aacagacaat ttgattcgtt atgaagaagc tcaagctcgc
     1201 aaaaagcatg gatgggtaac agtaaatgga gcagatactg tagttacgga tgaagcaggc
     1261 aaataatatg ggattcttta tggctaaaat tctcttgttc ttaggtttag taggagcagc
     1321 atatctcgtg tatgcgctcc tttcccaaac tgatgacaaa gaagatgaca acaacgatga
     1381 aatgaaattt taggaggaga aataatggac gaactaatca caaaagtaga gcagtgggct
     1441 aaagacaagg gactggatca agcagatcca aaagcacagt ttttgaaagt agctgaggaa
     1501 ttcggggaaa tagcttcggc gatggcaaga agtaatgatg aactatttaa agatagcgta
     1561 ggagacgtta tcgtcacgct gattatcctt tccatgcaaa aagggacaaa cgtacaagag
     1621 tgtttagaaa tggcatacaa cgaaatcaaa ggacgcacag ggaaaatggt agatggtgta
     1681 ttcgtgaagt cgagtgattt ggaggatgtg aaatgaaata cgaaatacca ctaagtgaag
     1741 cgggcattca agcaattatc aatggtcggg aggttaacat agaacttcct gatggtactg
     1801 aattagtcat cagacaaagt tatttgaaag atatggcagc tccagtatta attgatcgtt
     1861 ttaacgtgac tgattctgtg gtagagaacc acttaaaaga atttcgatca agtatagaca
     1921 acactttcag attagggagt tgattgacaa tgaacaccag acaccgcaga gtagcaaaac
     1981 taagaaaaca ggaactaaat gtactaaaga caaagtttga aaaagaatat ggaatttcag
     2041 cagaagaaac atataaagtg gcaagtcagt ttgttgctga tgcaagtgat gctattcgta
     2101 agtttgggat ttcgatatta aatgatgatc gtaaatggga ggaaatgaga tgaaactaaa
     2161 agacggattt tacgctagta gtcacggcat cggcggttta atgctagata tgccgacaaa
     2221 gaaccctaaa acacgtaaga aaccaaaagt caaagtcggt gacatggttc gctgtgaagc
     2281 agaagagttc atttatccgt ttcgtggata tgtagaacac gtctataatc actcagcgat
     2341 cattcgtatt gaaaacacaa tggaatgcga taagtggtta gcgaaaagca aagagaattt
     2401 agctgtagcg agattggtgg atatggaggt tataaacaat gaagtttaaa atctttgaag
     2461 aggacactcg ttataaatta gaaaaagaat taaacgattt tgcgaaaaac aatgagattc
     2521 agcatatatc tttagcaact tctaagagag gttatgcaaa ttactatgca gctgttgtga
     2581 gctatgtaag tcgagaagtg taactcggca aataaaaaaa gccggatcgc tccgactgat
     2641 tcaataaatc caacac
//
LOCUS       DACEMO010000241         2576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEMO010000241 DACEMO010000000
VERSION     DACEMO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.248"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            44..139
                     /locus_tag="IW728_002560"
     CDS             44..139
                     /locus_tag="IW728_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1025887.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(441..686)
                     /locus_tag="IW728_002561"
     CDS             complement(441..686)
                     /locus_tag="IW728_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025888.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1031..1315
                     /locus_tag="IW728_002562"
     CDS             1031..1315
                     /locus_tag="IW728_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025889.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1318..1734
                     /locus_tag="IW728_002563"
     CDS             1318..1734
                     /locus_tag="IW728_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025890.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1871..2548)
                     /locus_tag="IW728_002564"
     CDS             complement(1871..2548)
                     /locus_tag="IW728_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1025891.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT          864 a          463 c          393 g          856 t
ORIGIN      
        1 taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt tcgtgttcac
       61 taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt actggttgaa
      121 taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa aagccccaga
      181 gaatccgacc tctctggggc tttcgtgtgt ttcgtgttca cttatttgca atcaaattat
      241 aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc gtaattattt
      301 cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc gatttaagac
      361 gttttattta gtttttggtg tatttcattg gtccaagcta aaaaactctt agaagcgatt
      421 ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca tgcgtcaaaa
      481 gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc caacgatcgt
      541 tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa atcgaacttg
      601 attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta aggaaatttt
      661 tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact tctttgccgt
      721 ctatactctc aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt
      781 aaatttggtt atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa
      841 acgtgtaccc actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga
      901 aaaaaatttg atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa
      961 aaacagctat acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag
     1021 gagatttttt atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga
     1081 caaacgttgc caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct
     1141 aaaagtttta atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt
     1201 cgatttattg ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga
     1261 gtatgtctac acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg
     1321 agaaaaactt ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat
     1381 tccatcatcg aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt
     1441 atgagttatg aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct
     1501 atgggaaaag aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta
     1561 aaagatacag gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa
     1621 atcgaagcta aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc
     1681 agtcaaacag tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca
     1741 gataggagtt attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta
     1801 ctagcaatct tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt
     1861 tcttttttat ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta
     1921 ggcttgaaaa agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg
     1981 tgactttaag atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt
     2041 gatcaatgtt acccatttga tagtattctg ggatatccat taagctccta aactcttcaa
     2101 tacgaactac ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg
     2161 catacgtaga acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta
     2221 aaccattaat aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt
     2281 tattgatttc aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac
     2341 ggtaagtaag attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg
     2401 tagcagtcat cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt
     2461 tcaaaccacg atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat
     2521 tgaaattttt cagcggtact aaattcaatt cattttgata tttgactgtt aattcc
//
LOCUS       DACEMO010000242         2574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.249, whole genome shotgun sequence.
ACCESSION   DACEMO010000242 DACEMO010000000
VERSION     DACEMO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.249"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            168..563
                     /locus_tag="IW728_002565"
     CDS             168..563
                     /locus_tag="IW728_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025892.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            888..1505
                     /locus_tag="IW728_002566"
     CDS             888..1505
                     /locus_tag="IW728_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1025893.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1546..1845
                     /locus_tag="IW728_002567"
     CDS             1546..1845
                     /locus_tag="IW728_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025894.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1879..2046
                     /locus_tag="IW728_002568"
     CDS             1879..2046
                     /locus_tag="IW728_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1025895.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(2091..>2574)
                     /locus_tag="IW728_002569"
     CDS             complement(2091..>2574)
                     /locus_tag="IW728_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1025896.1"
                     /translation="ERPEFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKK
                     VALKILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAG
                     RYKGRPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL
                     "
BASE COUNT          911 a          376 c          485 g          802 t
ORIGIN      
        1 tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa ttattcaatt
       61 tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag tgtccttttc
      121 ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg atgattatgg
      181 aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga tgtggaatta
      241 tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc aagatagtag
      301 attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag tttgaaagag
      361 taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa gagtctccaa
      421 atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa aagaaagaat
      481 acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct aaattagaaa
      541 tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa agaagtcaaa
      601 ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg gataactagt
      661 ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg aataaatata
      721 atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat gccttgattt
      781 tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt ctgatttaat
      841 ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg agaaaatttt
      901 tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt tccgcgaagg
      961 aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa gattttaaac
     1021 gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc tatctacaat
     1081 cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga gaattaacta
     1141 aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact gctagttaca
     1201 agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta ctcagctata
     1261 tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta gaagctgcga
     1321 aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa tttcctgcga
     1381 tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat caattgggca
     1441 tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg caccactttt
     1501 cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga cttaacgaat
     1561 caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc catcggtttg
     1621 gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa tttcctggtt
     1681 tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa ggaacaaacc
     1741 gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt cgaattttat
     1801 gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt agtaaaattt
     1861 acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat tgaaataact
     1921 agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt agcaaaaagt
     1981 gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg ggtacatgga
     2041 acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt ctacaaatca
     2101 ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt tgcaatctct
     2161 gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa gcgtttttgc
     2221 ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc ggctttttga
     2281 gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt ttgtgcaata
     2341 taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc atttccagta
     2401 ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg tttcataaaa
     2461 gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga tgtaacgatg
     2521 acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg ctca
//
LOCUS       DACEMO010000243         2561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.250, whole genome shotgun sequence.
ACCESSION   DACEMO010000243 DACEMO010000000
VERSION     DACEMO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.250"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..157
                     /locus_tag="IW728_002570"
     CDS             <1..157
                     /locus_tag="IW728_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1025897.1"
                     /translation="NKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKDF
                     MNTFKTI"
     gene            229..1449
                     /locus_tag="IW728_002571"
     CDS             229..1449
                     /locus_tag="IW728_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1025898.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKTVFEAFQKGLKN"
     gene            complement(1542..1934)
                     /gene="rpsI"
                     /locus_tag="IW728_002572"
     CDS             complement(1542..1934)
                     /gene="rpsI"
                     /locus_tag="IW728_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1025899.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1950..2393)
                     /gene="rplM"
                     /locus_tag="IW728_002573"
     CDS             complement(1950..2393)
                     /gene="rplM"
                     /locus_tag="IW728_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1025900.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT          878 a          446 c          448 g          789 t
ORIGIN      
        1 gaataaaaaa caaacctgtg agtttctagg aattgataat aatactttag ataagtggat
       61 tcgtatatat aattttccag taatcagaat tgacggtata gttcgctttg acatagagga
      121 tatcaaagat tttatgaata catttaaaac aatttgaaaa tttcttctcg ccgcatatac
      181 taagaatagc gaaatatact gccttacttt ttaagggagt gaggaatttt gcctacaaaa
      241 ctttcaaatg ggaaatataa aactaatctg aggtatccaa aaaaattcag agaaatcact
      301 ggaattacat cagaaaagta tcaaaaagta tttccaacta ggcaattggc tattaaagcc
      361 gaaaatgcaa tgaaaaggaa aattgaaaca gttttaagag aggagaatgc aaattcacta
      421 gaattaaaag gaaaaataaa atttaaggaa ttctatgaat caaaatggct tcctagatat
      481 gaactaggtc agactactag atcaaaaaga gctccaagtt acgtaacaat tagtaatact
      541 aaagatatat tccgattgca catccttcca atgtttggtg agtatgcaat gaactatcta
      601 aattcaaata ctgagataat atcagatgaa ttaacaaaaa aatcgaaaga atacgcaaat
      661 ataaaaatta tcaaaggata tgtccgttct atgtttgata ttgcagaaat actaaattat
      721 attgaattta accgaactac caaaattatt caatgtatca ctgcacctaa aaaaattgct
      781 ttagaagaaa gaaggatacg ggaaggtaat caagcattat cgtcagaaga gcttattgct
      841 tggatagaag cagtaaacac tgacttgaat aatcacttgc ttactcttca tgactatact
      901 cttttcatgc ttaccctata tcttggtgat agaaaaagtg agacgtatgc tttgcaatgg
      961 aaatatatcg actttgagaa acaaacggtt cgccttaaac atgctttgga taaatatcaa
     1021 aaaaagaaat ttacaaaagg tcgaaaggat accgtaattc aagtacccga aattgtaatg
     1081 gcattactaa gcaaatggaa atcagtgcaa gctgagcaat tgttgagatt aaaaattaat
     1141 caaacaccag atcagtatct ctttacctat actaaaccat ccggagaagt aaattgccct
     1201 gttcacgctg attacctaaa ctatcgaatc aattcaatta aaagacgaca tcctgaattg
     1261 gctcatttat ctccacataa gcttcgtcat acctatgcaa ctatagctag acaaggcgga
     1321 gcaaatatga atcaaatatc taatgcttta acacactcag atatttctac tacaaagata
     1381 tacgtaaaca caccagatgt agttgataag actgttttcg aagcatttca aaaaggatta
     1441 aagaactagg atgaatgtgt tctcgaatgt gttcttaaac taaaaaaaga cactttccaa
     1501 ataattgaaa agtgtcttga aacattgata tacaaagaat attaacgttt tgaaaactgt
     1561 gaagctttac gagctttttt aagacctggt tttttacgtt cgaccatacg tgcgtcacga
     1621 gtaagtaagc cagcacgttt aagagctaaa cggaagtcag gatcaacttc tagcaatgca
     1681 cgagcgatac catgacggat agctcctgat tgtccagcgt atcctccgcc gtttacgtta
     1741 acaaatacgt catatgcgcc ttttgtttca gtaacagcga aaggttgatt gattacttca
     1801 cgcaagtcag catgtggaat gtattcttca acatcttttt tatttacagt aattttgcca
     1861 gttcctggta ctagacgtac gcgagcaact gcgtttttac gacggcctgt gccgatatat
     1921 tgaacttgtg ccaatgaaat tccctcctat tagattaagt ttgtgatgtc taaaacttct
     1981 ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata cgttcaattt tgtaaattgt
     2041 ttgcggccta aagtgttttt aggaagcata ccttttacag aagtttcgat caaacgacga
     2101 gaatttttag cacgcaattc gccagcagtg attgatttca atcctcctgg gtagttgctg
     2161 tgacggtaat aaattttgtc agtcgctttt ttacctgtta atttcacttt gtctgcattg
     2221 atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga aagttggttt atttttacca
     2281 cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg gaacatcagt tgcgtctact
     2341 acataccatt tacgttctac ttcgcctggt ttggccatat atgttgtacg cacgttattt
     2401 tcctccaatt taagttctga tgtttgacat acacttcgag tttccggggc tctttgtggg
     2461 gcaaacaata ccatttaaca tattacccgc taatctagtc aatgtcaacg tttttctgct
     2521 tgattgaaaa atttatatga aactttactt cttttctttc t
//
LOCUS       DACEMO010000244         2516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEMO010000244 DACEMO010000000
VERSION     DACEMO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.252"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..399)
                     /locus_tag="IW728_002574"
     CDS             complement(<1..399)
                     /locus_tag="IW728_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1025901.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENL"
     gene            512..1432
                     /locus_tag="IW728_002575"
     CDS             512..1432
                     /locus_tag="IW728_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1025902.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            1505..2440
                     /gene="murB"
                     /locus_tag="IW728_002576"
     CDS             1505..2440
                     /gene="murB"
                     /locus_tag="IW728_002576"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1025903.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
BASE COUNT          889 a          414 c          533 g          680 t
ORIGIN      
        1 tagattttcc aaataattat aaaaggcttc ttcccattct aatcggtaat ctaaacgttt
       61 taattcgctt tgagaatttg gcgtgtaaca ggttacaaga aagaaatcgg aatattccaa
      121 agtaatcaga cggccttctg tatcatggat atcgataccc attccgtaag tggcattcaa
      181 tgctggtttt ttagcaaaaa tcgctgtgcc ggaatagcct tttcgttccg cgtaattcca
      241 atattgataa taacctggaa gatctaaatc aatttgtcct gcttggagtt tcgtttcctg
      301 cagacagaaa aaatcggcgt ctaactcatg aaaaacttct aaaaaatttt tatttacgat
      361 ggcacgaagc ccgtttacgt tccaagagat aaatttcaat aaaagcacac tccttcgaat
      421 taatttcctt acctttgcga tactattgta cctcatttta gtaaaaaatg gtgtaagatg
      481 aaagtaacta aatgatccag gaggaaagta gatgaagatt ggtgtaatcg gtgtagggaa
      541 tatcgcagaa aaagcatatt taccaactta tgcgaaaaaa caaggaacac ttgattttta
      601 ttttgcaaca agaaataaag aaacaaaaga aagaattcaa aaagagtacg ggttccaaca
      661 tctatatgaa acgatcgatg agcttttaga agaaaaaatc gaagcatgta tgatccacgc
      721 agcaacaaaa gtacattatg aattagccaa aaaatgtcta gaaaatggta tccatgttta
      781 tattgacaaa ccactgagtg tccatctaaa tgaagttcgt gaattgcaag aattagctga
      841 taagaaccaa ataattttga tgatcggttt caaccggcgc tttgctccta tggtagaaga
      901 attgaaacgt atcccagata agcggttgat ccacctgcaa aaaaatcgaa tagcggcaaa
      961 agaaacgaca gagttcgtcc tttatgattt gtttttacat cttattgata cagctgtgta
     1021 tttattagat gaacccattt tgagaacaaa ataccagata agagaaacaa aggaattttt
     1081 ggaatatgcc attttacaat tagaaacaca aaatcaaacg gctattttaa cgatggattt
     1141 agcaagtggc gctaatatag aaaaatatca agtaacaagt aaaaatggta cttatgaagt
     1201 aaacgatctg acccagcttg tcattcaaga tgctgatgga aaaaaagtga aagaatttgg
     1261 cgactggaca aacacgctag tcaaacgcgg gtttgagcca atggtagagg ccttcttttc
     1321 tcagatccgt ttaggtaaat cggaaacaga attgcttaaa caaaaagaag ttgtaagaag
     1381 tcatgaatta tgtgaggaag tgcttaaaga tcactaccgt catcaacttt gaacaagact
     1441 ggcaagattg tattaagcgt gatagaataa tgaggacgtt tgagaaaaga aaggaaaatc
     1501 ctcagtgaat aaagaagaaa tagttgaaag cttagccacg attactatat tgtttgatga
     1561 accattgatg aactatacgt ttacgaaaac aggtggacca gctgatgtcc ttgcattccc
     1621 gaaaaaacaa gaagaagtaa agcaaatcat cgattattgc cgaatccatg atataccttg
     1681 gatggtatta ggaaatgcaa gcaatctgat cgttcaagat ggcgggattc gcggtgtagt
     1741 catcatgctg actgaaatga aacagattca tgtcaaagga acgatggtca ttgcggaagc
     1801 aggtgcctca ctgattgata cgacttatgc agctttggca gaatcattga ctggatttga
     1861 atttgcttgc ggcatcccgg gaagtgtcgg tggagcggtt tatatgaatg caggtgcgta
     1921 tggcggagag atcaaagatg tttttgctga agtggatctt ttgcttgaag atggaacatt
     1981 gaaaactttg acaaaagacg aaatggtatt ttcttatcgt cacagtaaag tccaagaatt
     2041 acgtgctatc gtattggaag ctcgattcag tttgcaaaca ggggattatg aagcaatcaa
     2101 agcaagaatg gatgagttga ccgaattgcg tcaatcaaaa cagccattag aatacccttc
     2161 atgcggaagt gtctttaagc gcccagtagg acactataca ggtcagctga tccaacaagc
     2221 aggattgcaa ggcctgaaat ggggcggtgc acaagtctct gaaaaacatg ctggattcat
     2281 cgtcaatatc gatcatgcga cagcgacaga ttatattgaa ttgattgcgc acatccaaga
     2341 agtaatcaaa gaaaaatttg atgtgtcttt agaaacagaa gtgcggatca ttggagaaaa
     2401 acaagcaata tcagaaacaa gcagaagttt agccaattaa taagtaatgg aacttaaaat
     2461 aagtaaaaga gcaggacggt ggccgtcctg ctcttttttg tggttgttta cgcaaa
//
LOCUS       DACEMO010000245         2495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.253, whole genome shotgun sequence.
ACCESSION   DACEMO010000245 DACEMO010000000
VERSION     DACEMO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.253"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            158..343
                     /locus_tag="IW728_002577"
     CDS             158..343
                     /locus_tag="IW728_002577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025904.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            467..1183
                     /locus_tag="IW728_002578"
     CDS             467..1183
                     /locus_tag="IW728_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1025905.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1085..1605)
                     /locus_tag="IW728_002579"
                     /pseudo
     CDS             complement(1085..1605)
                     /locus_tag="IW728_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1893..>2495
                     /locus_tag="IW728_002580"
     CDS             1893..>2495
                     /locus_tag="IW728_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1025906.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQL"
BASE COUNT          791 a          385 c          546 g          773 t
ORIGIN      
        1 atgctcaaaa ggactgatag aatgatttac tatcagtcct agaaattata gcgccacatt
       61 tctttcacct ttgacttttc agacggcagt ttttatgctg atactgtaga gagagcagga
      121 cacagctttt gcttgttttg ggtaaggggg aaagaagatg accaaagcac ttatttttga
      181 ttttgatggg acgatgattg acagtgaatg gatgttttac gagagtattc atcactattt
      241 actgaagcct atttactgga gcagttcggc tataaaattc cttttgcaga ctatcaacaa
      301 ggtgttggga cggcagcaga actgtccttt tatgaaggga taagtcaagc ggttgggaga
      361 ccagtggatg ctgaacattt tgagcagcgg atcagcagca ctggaaaatc ggagtagtct
      421 caattctgaa ctgcgggagc tagactattt ttttgaagca catccgatga ttaaggaaaa
      481 aattgattcc tttgtgacga tcgatgatct tcaggtcggc aaaccagatc cagaaggtta
      541 tttgttgtgc ttgaaacggc tgagggttga acaggctgtt gtagttgagg atagtccagt
      601 cggtgcccaa gcagcaccgc atatgacagt tgatgtacaa gcgttggatg cagatttttt
      661 cgcctttagc gggcataaaa tgtgcgggcc gacaggaatc ggtgtattat atggcaaacg
      721 tcaattgtta gaacaaatgg agccagtcga attcggcggt gaaatgatag attttgtcaa
      781 tctttacgat agttcatgga aagagctgcc atggaaattt gaagctggta ctccgaacat
      841 tgcaggagca atcgattact tgaatgagat tggtatggag aatattcatc gttatgagca
      901 agaactggtt gcctacgttt tacctaaatt acaagcgatc ggcggtatca ccatttacgg
      961 accgcaagat cctgaacatc atactggcgt ggttgctttt aatttggatg gcatccatcc
     1021 ccgtgatgtg gcgactgctc tggatatgga aggtgtcgct gtacgttcag gacaccattg
     1081 tgcacaacgg ttcatcgacg atttctgttt gactaattgg tcgtttaaac agtcgatgat
     1141 aatgccgttt gagcggctcc aatgtcgtgg aatctttgag taagagtttc caatatcgct
     1201 tgagccgacg gtacttcttc tgattttcag attacgtgat tgattttcgt acagttctat
     1261 gagaatcgcc tgcttcagtt cctttgttag ttgatgatgt tcatcaacta agtccgtttt
     1321 cgcagaaaag gtctgcccac aatcatagca tagaaaacgg gtacgtttta attctaaaat
     1381 agtcaacttt tctttgactt tcaacatctg agtacgtgtc gtataggaac cgttgcggat
     1441 aatacgaatc gatccacagt gttcacaatg ggaaggtgag tcatccaatc tccctttaat
     1501 aaactgcgct tgaacgcctt tatattcttt ttcaatcagc cattcttcat caaaaaataa
     1561 atgtttatct gttaatccga gtaattttct agtatgatta tccatagagc gactcctttg
     1621 gtagggtgat gttttggtcg actaaatttt accaaaagtg agtctctttt tgagttgaaa
     1681 cgaagaaaaa gtgttagtag aaaatcgcct tcgattttcc aacaacacaa aatattatag
     1741 aagccttttt tctaaaggat ttcaaggtgt ctgtttgcat ttattaaaaa aaagaaaaag
     1801 tattggcaac agaaggcgtc accaacacta gatattatag agccataata tgctgtataa
     1861 tatctagtgt ataggcttaa gtggaggtat atatgaaaat attaactgtt gaagatgata
     1921 atatgataag agagggaata agtgaatatc tttcagaatt tggatatact gttattcaag
     1981 ctaaagatgg aagagaagcc ttgtcaaaat ttaatagcga tataaatttg gttatcttag
     2041 acattcagat accttttata aatggtttag aagtgttgaa agaaattaga aagaaaagca
     2101 atttaccaat tctaatcttg actgcattta gtgatgaaga atataaaatt gatgcattta
     2161 ctaatttagt agacggatat gtagaaaagc cattttcatt gccggtctta aaagctagaa
     2221 tagattcttt aattaagaag aattatggac atttagagaa gtttgaatat aaggatctat
     2281 cggttaattt taatagctat acagctaaaa taaatggtga agaaatagat gtaaatgcaa
     2341 aagaactaga agtattaaaa tgtttattgg ataataatgg acaagtgtta acaagaatgc
     2401 aaattattga ttatgtatgg aaagatagcg aagaaactcc ttatgatcga gtaatagatg
     2461 tatatataaa agaacttaga aaaaaattac aatta
//
LOCUS       DACEMO010000246         2491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.254, whole genome shotgun sequence.
ACCESSION   DACEMO010000246 DACEMO010000000
VERSION     DACEMO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.254"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(31..621)
                     /locus_tag="IW728_002581"
     CDS             complement(31..621)
                     /locus_tag="IW728_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1025907.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            1105..1332
                     /locus_tag="IW728_002582"
     CDS             1105..1332
                     /locus_tag="IW728_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1025908.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1348..>2491
                     /locus_tag="IW728_002583"
     CDS             1348..>2491
                     /locus_tag="IW728_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1025909.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVF"
BASE COUNT          838 a          417 c          489 g          747 t
ORIGIN      
        1 tgataaggag tatttaattg aagagaatga ttaggagaaa ggatctccgt actttttata
       61 gtaatcatag aatccaagtt tgtatagacg aatcgttttt tctcttattt cttctgtttc
      121 aaaatggact gcttgaagaa tcaagtctgt gaattctata ggagctgttc cattcgcagt
      181 aatcaaatta cgatctttca cagcttgttg ttctataaaa tcttctttat tctgataatt
      241 ttccgattct aaccaaggaa attgcgcatt tccggtgtgt ttgtaacctt gcaataatcc
      301 gctctgagct aaataatcaa ctgccccaca aatagcagct actggtacgt ctgtttttaa
      361 caatttatcg attatttgat ataaatgatg attttttatg tcccatgaat tgccaccgat
      421 cataataaaa aggtcaactt tttcaggtat ttgttcgatt aagtaatcga tttttgtttt
      481 taatcctcca atggaagtga cctctgattg gatagaagca gttttgtttt gccatttctc
      541 attttggttt agtgtgctag taatataagc accttcccaa tcggcatatt cctctaatag
      601 aaacgtaatc gcagttttca ttttcaagct ctcctttact atctataaca aggacaacag
      661 ttttaaccta cagacattca ttaaaattgg gtagcgacag cgaaaacaac aattcttaag
      721 ttttagacag agtatacaga tagtcaactt aattaaatac gaactaaata aaaatgacag
      781 gtaatcattt ttgtttacca gcattataac attttgcata catggatgac aaaagaacca
      841 attcccactt gcttgagtac aatattaaaa gaaactggaa ggcaaaaagt aattatagaa
      901 acgaaaaata aatcatatcg ggcactcaga catgaaaaca accctagaga tatataacta
      961 ggtaaccagt ttaatgaaag aaaaaatcat ccaagaagtc gattcttgga ttttttagtg
     1021 gatttgttcg ccaaatacgt ttagtatgcg tatatgagag gttgtaagca aagcgttgtg
     1081 gtctgagcac ttaaggagga atctgtgaaa atagcttttc atatttttgc agattttgac
     1141 ttaatgccga aggtcagctt tgcgattacc gtttatcgag aggctgtaaa agcaccgtgt
     1201 agctccgatg acaataagca agcattttta gagctaggtt tccttgattc taaatatgcg
     1261 gcttatttca atgagttggt gcttgaacgc gattttaaga aggtcgaaaa caggcacgac
     1321 ttttcgacgt aaggagaaag aatttcaatg atagaaacaa taaatcccgc agaaaacaag
     1381 gtttctgcgt acattcatat cccattttgc gagcatatct gctattactg tgattttaat
     1441 aaagtttttt tagaagggca gccagtagat gagtatgtgg aaatgctgct gaatgaaatg
     1501 aaaataatgc tagaaagtca tccgacagcg aatttagaaa cactttacgt agggggcggg
     1561 acaccaactt ctttatctgc taaacaactt gatcgactgt tatcaggtgc tagagagatc
     1621 ctgccttttc aaaatggtaa tgagtttact gtagaagcga atccaggtga tttgacccgt
     1681 gaaaaacttc aagtaatgaa aaactatggc gtcaatcgtt tatccatggg agtgcagaca
     1741 tttgataatc gcttattgaa aaaaattgga cgtaaacaca ccgcagaaga tgtgtatcaa
     1801 actatgcaat ttttagaagc agaaaatttt accaatgtaa gcatcgattt gatttatgca
     1861 ttaccgggac aaacactaga aggatatctg gatactttgg aaaaagcttt ggctttggat
     1921 ctgcctcact attctttgta ctcattgatc ctcgaaaaca aaacaatgtt catgaactgg
     1981 gtacgacaag gaagactcga actgcctgat caggaaacgg aaaccagaat gtttgaagag
     2041 acgattgaag caatggaaaa gcatggacgt caccaatatg aaataagtaa ctttgggctc
     2101 tccggacatg aatctcaaca taacctcatg tactggaaca atgaccacta ttttgggttt
     2161 ggagctggtg ctagcggtta tcttggagat attcgttacc gcaacaaagg accaatccaa
     2221 cactatttac gcccattacg agcaaatgaa cgcccggtat tggaagaaga aaaattgaca
     2281 cgaaaaaatc aaatagaaga agaaatgttc ttaggactaa gaaaaaaaac agggattact
     2341 aaaaaacatt tctatgatcg ctatcaaaaa acaattgaat cggtgtacgg tgatgtgcta
     2401 agggaattag agcaagaagg gttacttgtt aatgaagaag accgtgttta tttgacacca
     2461 agagggacct ttttggggaa caatgtgttt g
//
LOCUS       DACEMO010000247         2480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.255, whole genome shotgun sequence.
ACCESSION   DACEMO010000247 DACEMO010000000
VERSION     DACEMO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.255"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            186..2279
                     /locus_tag="IW728_002584"
     CDS             186..2279
                     /locus_tag="IW728_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1025910.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            2304..>2480
                     /locus_tag="IW728_002585"
     CDS             2304..>2480
                     /locus_tag="IW728_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1025911.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENF"
BASE COUNT          860 a          418 c          503 g          699 t
ORIGIN      
        1 tttgtataga tgggcaaaat ttttgtaata ataggcgtat gtctttttcg ttgaggagat
       61 gaaaaagcat atggcttatt ttttgtttgc tttgcttact ttctttccac ttcaagtggc
      121 agaaatatct actttttatt atgtaagcgg aaccatatac ttgaaatcag aaggagatca
      181 tttctatgaa aacaagaaca attgaaatac ttcacagaat catgacagcc caatatccag
      241 taaaagcaaa acatctagca agtgagttca atgtcagtca gcggacgatc cgtcaggaag
      301 ttcttgaagc gaatatctgg ttgagcagta aaaagcttcc ggagatccgt acaattcgca
      361 acaaaggttt tttgttgaag ctggaaaagg aaaagcaagc tcgattagaa actgaaataa
      421 aagatgtgaa agacggcttt ttgaatcggc gcgaacgtat ttttgatcta gttttagccg
      481 ttgcttacag ccgaatcccg atccatctaa acaaaaaaga agaagaattt caaatctcta
      541 aaagtacatt agatgaagat atgagacggt tacgagcaac actggtcagt tatgggatag
      601 agatcgtcag ttatggaaag caaggactgg tttataaagg ttcagaacga tctatccgta
      661 cgatgatcta tgattttatc aatcagaatc tgggaatgac cgacttcttt atgaatcaag
      721 atgcacaatt aacaccggct caaaaaatct ttctccatta ttttcctttg gtggaaatca
      781 aaaaaataca ggagatctat tccaataagt tgttgaaaca agaagatgat atttataaaa
      841 aacaaatcct gctttttaca ttgatctgga tccagcgaat caggaaacat gaatttatct
      901 ctgccattaa ttggaaaaat acagaaataa aagacagtag cttttctgca tttattgacc
      961 acatgatcga ggaatttcaa ttggacaatg tcccgcaagt agaaagaaat tatcttcgtt
     1021 tcactattga gacgttcaat gctagagata tctccaattc aatcgagtgg gtacaagctc
     1081 aattactaac gatccaattg atccaatttg tagagaacga gacacacgta cctttccatc
     1141 taaaggaaga agctttattc gaaaatctct ataagcacat ggctgcactg attgtaagaa
     1201 tcaagaatca tatccaagtc atgaatccat tgaaagaaaa catccgaaca aattatttcc
     1261 caatctaccg cgcagtcact cattttgttc ctacgatcga agaagtaatt ggtggcaaga
     1321 tactggagga cgagatagct tttcttgtca tccacttttc gacgatcgcc agtgtaatca
     1381 agcgagactt gaattatgtt tataaatcag tcgttgtctg taaccatggg atggctactg
     1441 gaaatctgtt agcagagaat ctgaaagaaa agtttcctca gatcgagatt actgcagttt
     1501 taagttcaaa agaaatcgaa ttagtagata aattggatgt caatctggtt ttcagcacct
     1561 ttcaattgaa ttatcgaaat aagccgcttt tagttgtcga tccgatttta actgagacga
     1621 atatcttggt tatagaagac tttttgaaaa gaaatgaaga actacagcgg ttagagccta
     1681 acagtcatga atcgacggag ttattcactc aaataataga tgtcatgaaa gaaagtggtg
     1741 gccgagtaga tcgaatcatc tatggcaagt tagaaaaatt attcgaaaca aacaatttag
     1801 aaatcaacaa aagggagatc cagcctatgt taaaagatat tttgacggac agcaatatca
     1861 tgatcaaaga aaaagccact acttgggagg aatcgattga actagctgct agaccattgg
     1921 ttaaagaaaa cattgtcgaa gagcgctatg tcgaagcgat gattcgtgca gtgaatgatt
     1981 acggaccgta tatcgtgatt ggcaaacaca tggcactagc ccatgcccga ccagaagatg
     2041 gggtcaataa attaggagtc agcgtagcta cgattgagca accaatcgat ttcggtaatc
     2101 ctgaaatgga tccagtaaag attatttttt gtttggcagc agtagactcc tactcgcatc
     2161 tgaacatcat gaaagaacta attgaattga tcaacaacga agagaagttg gatcgattga
     2221 ttgaatgcac aaatatcgaa gatttcaaac aactgctttt ttcagtagtt gagaaataat
     2281 aaatgatacg ggggagaaca aagatgaaca aattgaacat tttattcgta tgcggtgcag
     2341 gtttaggaag tagttttgca gcacagatgt ctgctgagga tgttttgaac aaattaggag
     2401 tagaagccaa actggaccat tgtgatattt cttccgcagt ttccatgaaa ccagatgtga
     2461 tcatcacagc agaaaatttt
//
LOCUS       DACEMO010000248         2471 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.256, whole genome shotgun sequence.
ACCESSION   DACEMO010000248 DACEMO010000000
VERSION     DACEMO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.256"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..418
                     /locus_tag="IW728_002586"
     CDS             <1..418
                     /locus_tag="IW728_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1025912.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            632..1327
                     /gene="vanR"
                     /locus_tag="IW728_002587"
     CDS             632..1327
                     /gene="vanR"
                     /locus_tag="IW728_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1025913.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1305..2459
                     /locus_tag="IW728_002588"
     CDS             1305..2459
                     /locus_tag="IW728_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1025914.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          853 a          443 c          521 g          654 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc
      421 attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta
      481 acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa
      541 ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt
      601 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      661 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      721 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      781 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      841 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      901 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      961 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
     1021 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
     1081 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
     1141 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
     1201 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
     1261 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
     1321 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
     1381 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
     1441 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
     1501 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1561 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1621 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1681 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1741 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1801 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1861 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1921 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1981 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     2041 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     2101 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     2161 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     2221 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     2281 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     2341 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     2401 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     2461 agatgtatat a
//
LOCUS       DACEMO010000249         2460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.257, whole genome shotgun sequence.
ACCESSION   DACEMO010000249 DACEMO010000000
VERSION     DACEMO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.257"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IW728_002589"
     CDS             complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IW728_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1025915.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTE"
     gene            complement(734..1573)
                     /locus_tag="IW728_002590"
     CDS             complement(734..1573)
                     /locus_tag="IW728_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1025916.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1758..>2460
                     /locus_tag="IW728_002591"
     CDS             1758..>2460
                     /locus_tag="IW728_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1025917.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYY"
BASE COUNT          866 a          503 c          368 g          723 t
ORIGIN      
        1 tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg
       61 tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc
      121 ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt
      181 tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa
      241 taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc
      301 cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt
      361 ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt
      421 tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca
      481 ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt
      541 ctatcactct ccatataatt accttctatt tgataaagga attatataaa atgaaaatga
      601 tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt
      661 tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg
      721 ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg
      781 catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt
      841 cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa
      901 aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccaattcc
      961 aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga
     1021 aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc
     1081 cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa
     1141 gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa
     1201 ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg
     1261 cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac
     1321 ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct tctattcttt
     1381 ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg
     1441 taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat
     1501 tgacaagaat tcctaaaaca aaaccaacta agactattaa aacagatcca atcaaccgaa
     1561 taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg
     1621 tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt acgaactaag
     1681 aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg
     1741 tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt
     1801 cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt
     1861 ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata
     1921 atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag
     1981 agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa
     2041 actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta
     2101 aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc
     2161 tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga
     2221 ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggggaaaat ttctccaagc
     2281 ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg
     2341 attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg
     2401 gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc
//
LOCUS       DACEMO010000250         2443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.258, whole genome shotgun sequence.
ACCESSION   DACEMO010000250 DACEMO010000000
VERSION     DACEMO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.258"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            109..408
                     /locus_tag="IW728_002592"
     CDS             109..408
                     /locus_tag="IW728_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1025918.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            673..1548
                     /locus_tag="IW728_002593"
     CDS             673..1548
                     /locus_tag="IW728_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025919.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1545..1763
                     /locus_tag="IW728_002594"
     CDS             1545..1763
                     /locus_tag="IW728_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025920.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1750..>2443
                     /locus_tag="IW728_002595"
     CDS             1750..>2443
                     /locus_tag="IW728_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025921.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQ"
BASE COUNT          960 a          281 c          464 g          738 t
ORIGIN      
        1 ttattcttat aaatacaaat aaatacattt ttttacgcaa tttgtattta tttgtactta
       61 taaaggtaga ggagttatag aataattaat taaaaaggag aggtttttgt ggaagcaata
      121 aaattaatga tttcagaaga acaggaggaa atgtttctaa agcagtttta tcaaattgct
      181 acaaaagcta ttgaaaaggc tcaagaaaat gcggggatga caagggaata tctcaatcaa
      241 aaagaaatgg cggaatactt aaatgtgagt gaaaactttt taaaaaatgc tgtgagaaat
      301 gagggattgc ccgtagttga attaggggca agaaaatttt attcgaaaaa ggcagttaat
      361 gagtttatgt ttgctcgaat gaaaagaggg gaaaatattg gaagatgatg aagtagaaat
      421 tataaaaaaa tgctttaaaa aaagaaaaaa agtgttccca aaagtttgca gactggggga
      481 acactcaata ataaagtgct gagaaaactt tatttatagc tattatatca aaaaagaagg
      541 tgaaaaggtg ttttcttctg gaaatgttgt agttgacaaa cttagagaaa taaattttga
      601 cggaaatgta atccacacac atggtacgat caattaagaa aaaaaacgaa aaaaggtgtt
      661 gagaagccct atttgaatgc aatagttatc ttagcagaaa ttacctattg gtatagacct
      721 aaaaaaattt ttaacgatga tggtcagcta cttggttatc aaaaaaagtt tcttgaagat
      781 attttgcaaa aatcatataa gcaattaagc aaaaaaacag gattatcaga acgagctata
      841 aaagatgcaa ttgtgtactt agagaaaaag gggattatca aaagggtttt tagaaatatt
      901 tattatgaga aaagagcagt tacattatca aatgttatgt acattgtttt aaatgtacat
      961 aaattgatag aaattacttt tcctactttg gattgcgaaa aaaaggagta tatgccgaaa
     1021 attcgtcaga ggggtatcga aaaaaagaga tatgtctctt taaaaaaagg tagaggtatg
     1081 tacaaatttg tatatacgta tacaaagact acttcacaga acatatcaaa gactactctc
     1141 gttatggaag aagaggatat taatataaag aggatttaca gtaagattag tgttctttat
     1201 gacgcaaata acgaagatgg tcgaaaatta tttcaagtgt atcactattt gagaaacgct
     1261 aatgtagata cagaagatat cagaaaaatc attgaatatt tatctgaaaa tactcagtat
     1321 cttattgctg agtgtattat aaaacaacat gaagcatgct tttttcaaag aaaaactgaa
     1381 gaagggttat acgattatgt aacatatttt ttgaatggac ttcagaaaag atataaaagt
     1441 caatttttag aaaatgtaga gcctatagat cagttcaaaa aagctttcga aatcaaagag
     1501 ttacctgaaa ttactatgca taattggttg aaaggagaag aatcatgatt aaaaatcgct
     1561 taatagatca aatgtggtgt cttgatcaaa atacagatac tctcgaatta atcgagagta
     1621 tctgcttcaa cacagtagtt ctcgatttaa gagctgaaaa tgacaattgt gtaactcata
     1681 tcataattaa tcaaaaaatg gctcagcagc tctctgagag cctaagaaaa tgggctgaag
     1741 gagaaaacta tgaaagcaat taaagaaaaa atcgttagta gaaaaataaa ctggttagca
     1801 ctcagtgtac ttgttttaat gattagcgtt gtttttacaa gtaatattgt tgaagctagc
     1861 attttcaatt ggtttgatga cacaaaaagt caaacagagt ttttgaataa ttctctttat
     1921 agaccttacc taaaaaaata caatggagat ttccttagtc agttcgggat ttggcttgga
     1981 tgggctgtgg taaaaggcat gtttactgtg actgattcga ttcaaaatat gattccagat
     2041 gtgttggatt tgttcaattt cattgaaagt acagggctaa ataatgttta tcaatcagtc
     2101 ataaatacaa ttgtcgttgg attaatgatc ctttcattga tgtttgttgg ctacaagatg
     2161 attactggaa aaggaacaat agatttaaga tctgttggaa tgaatatagt gatgtctgta
     2221 gcgttaattc ttttaatgcc aacaatgatt tctagcggta ttcaatttgc caaaatattt
     2281 tacaatgatg ctactacgat tacaaattca gatgacggag tcgcttggtc attgataaaa
     2341 caaggagtaa cagatctggc atatattaat aaaacagatc aatactcaag cattgataaa
     2401 acggaggatc gaaataagtt aacacgaaaa aattttcaac aga
//
LOCUS       DACEMO010000251         2388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.259, whole genome shotgun sequence.
ACCESSION   DACEMO010000251 DACEMO010000000
VERSION     DACEMO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.259"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            177..359
                     /locus_tag="IW728_002596"
     CDS             177..359
                     /locus_tag="IW728_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025922.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            343..528
                     /locus_tag="IW728_002597"
     CDS             343..528
                     /locus_tag="IW728_002597"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ1025923.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            550..675
                     /locus_tag="IW728_002598"
     CDS             550..675
                     /locus_tag="IW728_002598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1025924.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            771..2291
                     /locus_tag="IW728_002599"
     CDS             771..2291
                     /locus_tag="IW728_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1025925.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
BASE COUNT          825 a          404 c          484 g          675 t
ORIGIN      
        1 tcaaacaact atattaatag gaagaaaact tcatttttcc tcttagttct ataaaaagtg
       61 tggctaattt gaggcaaaat attaaattaa atgtaagcgt gtcataggat agtatctcgt
      121 acgccgatta tctttcttct ataattaaac tatcaaatat agaaggagaa aaacgaatga
      181 ataaaaaaga tattgtacca gttcaatttt caagtgcggc tcaaaaatcg caaattgaca
      241 ccaagccaaa actagcttgc agaatcaaat cggaagataa ggaaatattt atttataatg
      301 gaatggataa atatcttttg tataccttgt taaaagagtt gaatgccaat gctcattgat
      361 gcaactgcat tgaaacatat ttttatcgtt tgtggaaaaa cagatcttcg aaaaggaatt
      421 gatggactag cagctttcgt tatagaagaa tatgaccttg atgtttacga tcaagcgctt
      481 ttcttatttt gcggtacgaa gaaagatcgc ttcaaggctc tttactagga tcaaaatgga
      541 tttgtattaa tgtacaaaag atttgaaagt gggtaccttc aatggccgcg ttccagaaaa
      601 gaagtcttac tgactggcct ttcaatcgaa gaaaaatgta cgctaaaaga aacgaaaaaa
      661 ggtgcgatat attagacgaa aaccttgtta aatcaaggat tttgtctgtt tttcgttgtc
      721 ataaaatggt ataatagatg taagaaaatc gctagaatgg tggtgtaaaa atgacaaaaa
      781 tggaatctga acttcttgta caaatcaagc aattaacaca gttgcttgaa atccagcaac
      841 aggaaaatac tcttcttaga gaacaaattg ctgaaatgaa tcgacgcttg tttggacgaa
      901 aaaaagaaac accacccgta gatgggcaaa tcgatttatt agacgactca acttttaatg
      961 aaccagagca cactggacaa gaaagccagg aacccattac ggtatcttct tttaaacgca
     1021 gaaaacgcaa gggactcaaa gcactttcat tagaaggtct gcctgaagtt cctattcatt
     1081 atgaattaaa aggtgccgat tgtttgtgtg aagactgtgg tcatacttta catgatatcg
     1141 gtgcgacaag gcttagagaa gagccattgt atgttcctgc ccatatagaa aaacaagtca
     1201 tctaccaaca tgcctatcat tgcagagaat gtgaagcatt aggagaaaca aaaatcaaaa
     1261 aagcgcctgt tcctaaaccg ttgatcaata atagtctagg ttcctcttca attgtgacac
     1321 agagtatttg ggaaaaattt gttaaaaagg tgccggcata tcgacaagaa aaagaatggc
     1381 atgcacttgg tttccctctt gatcgtaaaa aaatcactaa ttggcatatc aaggtaagtg
     1441 aaatggtttt agagccaatc tacaattgcc ttcatcaaga attaagcaat caggaggtct
     1501 tacacgcgga tgagacaagc tatcgggtat tgaatactca taaagaaaag accttttact
     1561 ggttatatgc ttcaggtaaa agcgaagaaa aacaagttgt gctctacgag cacgcaaatt
     1621 cgcgttccgt tgatgagcca aaaagttttt tagaatcatt tagcggtttc ttgcatactg
     1681 atggttacgc ggcctatgga aagctgccgg taaacaatgt ttattgttgg gcgcatgttc
     1741 gcagaaagtt ttttgaagca ttgggtaaac aaccgtcccc aaaaagtcat gccaaaatag
     1801 gattagactt ctgtgacaag atgtttcgaa tcgaacggac ctttaataag cttagcgcag
     1861 aagaaaggtt tgtagcgagg aaggaaacgt tgcagcctgt gatgactgaa ttttttcatt
     1921 ggtgtgagag ttttgcttat ttgccaaaat cgcagcttgg aaaggcggtt caatacgcag
     1981 tcaatcataa agacggactt cagattgtgt tgcttgatgg acgcttggaa ctttcaaata
     2041 accgagcaga gcgtgccatc aaagagttag tgatcggtcg aaaaaactgg ctattttcta
     2101 aaagcttaaa aggcgctaga tcaaatggaa ttatattgag tattatccag acagctgttg
     2161 cgaatgggtt aaacatccgc aaatatttaa atcatctttt tacagagatt cccaatctgt
     2221 cttcgatgac acctgaagcg ctacgcgctt atttgccatg gaatcagcag atacaagaaa
     2281 tctgtaaata atataagcca caaataacct gatttgagtg tatcaaatcg ggctatttgt
     2341 gtttaggtac cattctatga cacgcttacg ctcaaaacta ctgaatta
//
LOCUS       DACEMO010000252         2377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.260, whole genome shotgun sequence.
ACCESSION   DACEMO010000252 DACEMO010000000
VERSION     DACEMO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.260"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..248
                     /locus_tag="IW728_002600"
     CDS             <1..248
                     /locus_tag="IW728_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1025926.1"
                     /translation="VDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGF
                     FIVAYSGLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            248..1078
                     /locus_tag="IW728_002601"
     CDS             248..1078
                     /locus_tag="IW728_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1025927.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            1078..1725
                     /locus_tag="IW728_002602"
     CDS             1078..1725
                     /locus_tag="IW728_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1025928.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(1823..>2377)
                     /locus_tag="IW728_002603"
     CDS             complement(1823..>2377)
                     /locus_tag="IW728_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1025929.1"
                     /translation="KKESLKLAGVHGFSDSLSTRGHKIRYTVLHSKIIKADEFLANKL
                     QINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGSQYTSLYKLLEDDYDVK
                     ATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQPFHYSQSTVRCDRISY
                     VIEVTENITLDTVHKNDSDPKVID"
BASE COUNT          800 a          320 c          424 g          833 t
ORIGIN      
        1 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
       61 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
      121 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
      181 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
      241 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttcataa
      301 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
      361 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
      421 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgactcc
      481 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
      541 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
      601 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
      661 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
      721 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
      781 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
      841 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
      901 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
      961 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     1021 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     1081 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     1141 aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa
     1201 aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt
     1261 aacagtttag aattgaaggg aaaatttact aaatattgga aatcgcaaaa gattgattac
     1321 ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata
     1381 aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt
     1441 aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct
     1501 aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg
     1561 gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt
     1621 gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat
     1681 attaaatatc tgcgacaaga agtacaggtg aaatttggaa tataaataag ttttttgatg
     1741 tatttagaga atattagtaa aaataaaaat catgcccaaa tctaatcaaa attgattgct
     1801 ttgggtatga tttcttttaa tatcaatcta ttactttagg atctgaatca tttttatgca
     1861 ccgtatctaa agtgatattt tctgtgactt caataacata actaattcta tcacaacgaa
     1921 ctgtggattg agaataatga aatggttgat tatttttatc tttgccaacc ttttctaaaa
     1981 taaatagcgg atctcctact atacattgca ataagtcgga aagctcaggt gttgcagata
     2041 aaccgtttat ttccataata gagctcgtag ctttgacatc atagtcatct tctagaagtt
     2101 tatataatga agtatattgg ctaccttttt caaataaagt tggaaatttt tcttctgatg
     2161 cgaaaaaatt atcaattccc atcggggcac cgtcttccca cattaaacgt ttaatattgt
     2221 aaactctatc agttacattg atttgtaatt tattagctaa aaactcatca gcctttataa
     2281 ttttagaatg taaaacagta tatctaattt tatgtcctcg ggtagaaaga gaatcgctaa
     2341 atccatgaac tccagctaat tttaaacttt ctttttt
//
LOCUS       DACEMO010000253         2357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.261, whole genome shotgun sequence.
ACCESSION   DACEMO010000253 DACEMO010000000
VERSION     DACEMO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.261"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1102
                     /locus_tag="IW728_002604"
     CDS             <1..1102
                     /locus_tag="IW728_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1025930.1"
                     /translation="TRRGFNSKREAQLAYLEMEKQQRLKKKIVASNDKFSVVGEQWFQ
                     YYRSLNEQKEITYDKREEQLKIIVRWIGDKKLSDLTPECLQELLFTLKECGVNGTNVG
                     YAKNSLQSLIQVLNMIFKYCQRKNLISENPMKSVRMPKYQLTVTDLKEAINNIEDKFL
                     TVDELRTFLNYGIVHEELPMSVLFHVLFYTGCRVSEALALQPGDIDFKRNEILFYKQT
                     AVKGKSKDFRIETTKTVSSARRVLVTPLVMEKLQQLIKALGEMRQHYRFIIEETYLFV
                     YLDSSKRGVPYRREYVNDHVKRCVNRSGINKPFHTHLARHTMASLVAEHCSWEVLKDR
                     LGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES"
     gene            1505..1798
                     /locus_tag="IW728_002605"
     CDS             1505..1798
                     /locus_tag="IW728_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1025931.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          850 a          347 c          445 g          715 t
ORIGIN      
        1 aacaagacgt ggctttaatt caaaacgtga ggcgcaactc gcttatctgg aaatggagaa
       61 acaacaaagg cttaagaaaa aaattgttgc aagcaatgac aagtttagtg ttgtaggtga
      121 gcaatggttc caatattacc gttcgttaaa tgaacaaaag gaaataacct atgataagcg
      181 agaggaacaa ttaaaaatta ttgttcgatg gattggtgat aaaaagttgt ctgatttgac
      241 accagagtgc cttcaagaat tgctttttac cttaaaggaa tgtggcgtta atggaacaaa
      301 tgttggctat gccaaaaata gtttacaatc acttattcaa gttctaaata tgatttttaa
      361 atattgtcaa agaaagaatt tgataagtga aaatccaatg aagtctgtga gaatgccaaa
      421 atatcagtta acggtaactg atttaaaaga agccataaat aatattgagg acaagtttct
      481 tactgtcgat gaattgagaa cttttttgaa ttatggcatt gttcatgagg aattaccgat
      541 gtctgtactt ttccatgttc tattttacac tggctgccga gtcagcgagg cgcttgctct
      601 acagcctgga gatatagatt tcaaaagaaa tgaaattctg ttctataagc aaacggcagt
      661 taaaggtaag agtaaggatt ttcgaattga aactactaag acagttagtt ctgctagacg
      721 agttctagta actcctctag ttatggaaaa gctacaacag ttgattaagg ctttgggtga
      781 gatgaggcaa cattacaggt ttattataga agaaacatat ttgtttgtgt atcttgattc
      841 tagtaaacgt ggtgttcctt atcgtcgaga gtatgtaaat gatcacgtca aacggtgcgt
      901 gaatcgctca ggaattaata agccatttca tactcattta gctcgtcata caatggcaag
      961 cttagtagcg gaacactgta gctgggaagt cttgaaagat cgcttaggcc atactgacca
     1021 aacaacttcg aaaatttatc gccacttaac gtcaaatgaa aaggtaaaac ctctgattgc
     1081 ttttagttct ttagagagtt aaaaaaacgg tgaaagcact ggtattacag gattcttgaa
     1141 aaacgatttt ttaacaaatt agtcaatatg tagtcagagg taataaaaat ccttttatat
     1201 caatacttta acgacttttc acaagtatca aagaaatccg tcgttaatca ttgttatcag
     1261 actgagaaag aacccttggt attcaagggt tcttttttct tagcatgtaa tttgaacacc
     1321 ctgatccagt tttttcttta attattggta ggggagaata atggattaga ataaatctac
     1381 ctataagtag tagtaaaaaa aagagcacta tgataatatc tttcttaaga gtaaccgctt
     1441 taataaaaaa tccagaatat tctaacataa atggagtatt cttagaatca aaaggagaaa
     1501 gaagatggtt tatacttata cggagcaaga aaagatgaaa gttttagaca cattcatgaa
     1561 tgatggaaaa gtaaaacaag taccaagttc aacaaaaaag aaatacatcc tattgaaaga
     1621 agtcttgaaa cgctttgacc atggagtgac ttatacagaa acagaggtta attcgatact
     1681 gctgaacgta ttttctagtg gcgattatgt cgaacaaaga cgttatctaa tcacatttgg
     1741 ctttttcaaa agaagttcag acggtcgggc atatcagatg atgggaattg aaaattagaa
     1801 gttttaattt atactcattg aaaaaagttc aagtatgcga tattggttct acaccgcata
     1861 cttgaacttt ttttgtcatc tgtatctata atacaaagcc aagaacgtta ttcaaaatat
     1921 ggatagctat gataaaagct aatgagcgat tcgcagttaa ataagcaatc gtcagtaaaa
     1981 tcgttccagg agcgtacatg aaaaaatcaa ttggatgcgt tacatgaata aagataattc
     2041 tcttttattt gtacgaataa ataccaaaac gtacctccta aaattagatt ttggacagat
     2101 gccctttaaa aagataacaa gatttcgttc ttgaaacaat atatatgctt ttattccaat
     2161 gaggttgtga aaacagtatt ttgtcctgag cgttagaaaa ttgaaaatgg ggaatagcaa
     2221 ctcgtacttt accatttttt aagtgaatac aagatcagct agttgaacac cgtttattcg
     2281 tgatgaggga cacaacactg cagtcgacac atttctcttt tatgttcaaa gataacaaat
     2341 atttaaaaca aagaaaa
//
LOCUS       DACEMO010000254         2338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.262, whole genome shotgun sequence.
ACCESSION   DACEMO010000254 DACEMO010000000
VERSION     DACEMO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.262"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1034
                     /locus_tag="IW728_002606"
     CDS             <1..1034
                     /locus_tag="IW728_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1025932.1"
                     /translation="LSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSKKNNK
                     HKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNTTFEN
                     LTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFTAILV
                     KVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVGKFIR
                     AVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGYAQVY
                     GKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVISLAEI
                     AFPNEKIFR"
     gene            1050..2090
                     /locus_tag="IW728_002607"
     CDS             1050..2090
                     /locus_tag="IW728_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1025933.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2109..>2338
                     /locus_tag="IW728_002608"
     CDS             2109..>2338
                     /locus_tag="IW728_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ1025934.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEV"
BASE COUNT          845 a          363 c          425 g          705 t
ORIGIN      
        1 tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt cgagataaaa
       61 aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat tttggttcaa
      121 agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat gcaaacatca
      181 aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa gaaactgaaa
      241 aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg aatacaacat
      301 ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa agtattattg
      361 aaacatctgg atttagaatc caacctgaat atgaactatt caaacgtttg tttgatatca
      421 tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca gcaattttag
      481 tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca aaaaatcaaa
      541 aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca aaatctggac
      601 cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt attcgagctg
      661 tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg tcgatcgtag
      721 gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac ccttattact
      781 acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat ggaaaatatg
      841 tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa tattcattga
      901 tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa atgtcctctc
      961 aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt ccgaatgaaa
     1021 aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc ataatcgtac
     1081 cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa gaattggatt
     1141 atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca caggaaatca
     1201 ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc aatcaagggc
     1261 ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc ttattcttcg
     1321 atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg ctagaaaaga
     1381 gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac gagggaagaa
     1441 tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac gaagagttta
     1501 taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg aacaaatgct
     1561 atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat agttgtgagg
     1621 atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg aacccaaaag
     1681 tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac cgacctaaca
     1741 agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac gatattaata
     1801 aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt tctatttata
     1861 gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata ttgagtgatc
     1921 cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa aaaataacaa
     1981 agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt ggaagcttcg
     2041 ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag ttaatggagg
     2101 aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt ggagcagagt
     2161 ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt gattttttga
     2221 tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga ttaggaggcc
     2281 gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata gaagtgaa
//
LOCUS       DACEMO010000255         2338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.263, whole genome shotgun sequence.
ACCESSION   DACEMO010000255 DACEMO010000000
VERSION     DACEMO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.263"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..131)
                     /locus_tag="IW728_002609"
     CDS             complement(<1..131)
                     /locus_tag="IW728_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1025935.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVC"
     gene            complement(205..486)
                     /locus_tag="IW728_002610"
                     /pseudo
     CDS             complement(205..486)
                     /locus_tag="IW728_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            948..2330
                     /locus_tag="IW728_002611"
                     /pseudo
     CDS             948..2330
                     /locus_tag="IW728_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          847 a          395 c          393 g          703 t
ORIGIN      
        1 tgacaaacat ttattccacg atcatataac aattcctgaa cttcacgata gcttagattg
       61 taacgcaggt agtaaccaac agcgacaata atgacgtctt ttttgaattg tttgccttta
      121 aaatgattca ttactctgtc ctctctgtct tttttctcaa ttttacacta aaatagattt
      181 tttggaaaac tttgcaacag aaccgaaatg agtatagtat gaattagtaa tttggttttc
      241 atctaagttt agctttgatt tactaaaatt gatatctcga tacatgttca atacatctaa
      301 tacaaatcga gaatcttctt tcggaaatgg ctcagaaaga tcactccaaa tttcatcatc
      361 ataatgatac tcgtaacctt ctctcaatat ttcgattttt ctttcataat aagatctgtt
      421 ttggtcctca aatttagcca ataactcaaa ttgatttact agtgacaatc tctgaaaact
      481 gtccattaaa tgtttcttcc tatcttgttc attaacaaaa aattataagc ccttacaacg
      541 aatattatat attaaattta attgtaggtg aacacgaatt ttataaatat ttatggtgtt
      601 aatgacaaat gatatccgac tcctcttcat acgttctgga ctatttgcta atagattaaa
      661 atttcagcat ctatttctga ccataaataa atgagctgcc gaaaagctgc caaatcagac
      721 ttgaaaataa aggatcaaaa acttttaagt ttctgacccc gtaactcaca cttcaaaatc
      781 ttacttaaaa aggagtctga aagaaaaaaa gagtattcaa aatttttttc taactagcta
      841 catcacatct atgcattgac actcaaagaa ccagaataag tcatcagaaa gcaagggggt
      901 gcggacataa tcctggacga aaaaataaac ttaggaggaa tgtccgtgtg ttttcccaca
      961 aagagcgtgt aagggcaatt cagttatttt taaaatacga ttgctcttat gctgcaacca
     1021 ttcgagaatt agggtatccg tcgattggtg cactacggaa atggtataaa gagtatttgg
     1081 aatccggaga gctccatcag gagcaccgaa aaaaatcaaa gtattctgaa gaacaaaaac
     1141 gaatagcggt gaatcattat tttgaatatg gtcaatgtta tgcacgaacc attcgcatgc
     1201 ttggttatcc taatagagag tccttgcggc agcggtgtga agaacttgca ccaggagcta
     1261 gaaaactccg aaaaagtgcg gtaaagttaa cacaagatca aaaagaagct gtgttgaaaa
     1321 aattctataa accgcaaact aataggaaaa atttagccga atctggagga attagtagag
     1381 ttactctata tcaatggaaa aacacggttc ttggaaagga ttttccattg tgtatgactt
     1441 taaaaaatca ggaaaaacaa aaagagctac ttttaaagga agtagaagac ttacaaaaac
     1501 aagtacacca attacagctc gaaaaagcat tactcgaagg agcggctgaa ctgctaaaaa
     1561 aagaaaaagg cgtcaatctt ctttgtctat caaatcaaga aaaaacgata ttgattgatg
     1621 ccttacgaaa tcagttcacc ttgaaagagc ttcttcaaca gctccagttt cctaaaagca
     1681 gctattttta tcaaaaacaa gcacttgaga aaccagataa gtattacaaa gaacgtcaaa
     1741 ttaattatca cgattttcaa tcataacttc tgtgcgtatg gttaccgtag aattcaccaa
     1801 gctttaaaaa atatgggtaa aaagctttct gaaaaagttg tacgtagatt gatgactgag
     1861 gaaaatctat ttgttaaatt ttctagacga aaaaagtatt cttcgtatgc tggggaaatt
     1921 tctcctgcac accctaattt attaaatcgg aattttaaag caaagaagcc aaatgaaaaa
     1981 tggttaacag atattactga atttagaatt ctcgcaggga aaatctactt atcaccactt
     2041 gtcgattgtt acgatggtgc tattgttagt tggactattg gaacaaagcc aacttgcttc
     2101 actctatgtc taaaaagggt tattcaccag ataattcagc gtgcgaaggt ttctttggac
     2161 gattaaaaaa cgagatgttt tatcaacgtg actggaagaa tacaacaatc aatcagttta
     2221 taaacaaatt ggatgattat attcattggt ataataatca acgaattaag ttatctttag
     2281 gagggctaag ccctcttcaa tatcggaaga agcaaagata tatttgttaa aaaggtcc
//
LOCUS       DACEMO010000256         2314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.264, whole genome shotgun sequence.
ACCESSION   DACEMO010000256 DACEMO010000000
VERSION     DACEMO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.264"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(31..597)
                     /locus_tag="IW728_002612"
     CDS             complement(31..597)
                     /locus_tag="IW728_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1025936.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(597..1553)
                     /locus_tag="IW728_002613"
     CDS             complement(597..1553)
                     /locus_tag="IW728_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1025937.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1553..2209)
                     /locus_tag="IW728_002614"
     CDS             complement(1553..2209)
                     /locus_tag="IW728_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1025938.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          684 a          494 c          430 g          706 t
ORIGIN      
        1 aacatatcat ttatgttttg cgttttttat ttatcttttt ttctcaatac aatataaaat
       61 aggcggctgg ttcttttggt tgataaaccg ataattcagc acattatatg tttcctgtgg
      121 cagtttggaa caaaaagaat caaccatatc cagttctttt tctccacctt catgaccata
      181 gtagacgaca aggatcaatc gtccgcgagg cacaagtcgt ttcaatattt cttccatagc
      241 tgtgcgtgta gtctctggca tagtgatgat tgctttatca ctttttggga gataccctag
      301 attgaaaatc cctgctttga gattttgttg ttcagctatc acttctccta acgtttcgtg
      361 gccttgtaga aacaatgaga cacgttcttc taactgacgc tgttctagcc tttcacgagt
      421 agcatcgatt gcttgttgct ggatatcaaa actatagaca tgaccagttt ttccgatgtg
      481 ttctgctaga aaaaccgtat cgtggccatt gcccatcgtt gcatctacga cgtgatcccc
      541 tggttgcaag atttcttgca gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag
      601 acgctcacct ttttccgtac atcatatttt ccttggaagc tatctctgcg aagaagttct
      661 tcatcaattg cattcaagac ttcccatttt ttcaagctcc acattggtcc aatcaaggaa
      721 tcccacggcg catcccctgt caaacgatgg atgatgatct cttttggtat catttccagc
      781 tggtcgcaaa tgacattgac atagtcagga cggctcatta attgaaggcg accttcatga
      841 tagtcccgca acattctcgt tttgcgcatc agatgcatca aatggagctt gattccttgg
      901 atatctgaat ctaaaatggt gcgtctgaca ttttctttca tcatttctaa actttcgcct
      961 ggtaatccgt tgatcaaatg cgtacaaaca cggatattat gtttgcgtaa tttagccacg
     1021 ccatctaaat aagtttgata atcatgtgct cggttgatca gttttgaagt ttcttcaaaa
     1081 gttgtttgca atccaagttc tacccataaa tacatccgct cgttcagttc agcaaggtaa
     1141 tcaactactt catctggcag acaatccggt cgtgtaccga ctgataatcc tacgacacct
     1201 ggtaaattga ctacttgctc aaatcgttct ttgattattt caactggcgc atgggtgttt
     1261 gtaaaatttt ggaaataaac gatgtattgt tggacatgag gccatttttg atgcatcatg
     1321 tcgatttctt tctggaattg gattggaaga gggtcttcag gtgcaacgat catgtctccc
     1381 gaaccagata cactgcaaaa agtacaaccg ccatgggcaa cagtcccatc tcgattcgga
     1441 cagtcaaatc ctccatcgat cggcaccttg aaaattttcc cgccaaactc atttcgtaaa
     1501 gcgtaattcc aagaatgata tcttttattt ccttctgaat aaggaaaagt cattaaaagt
     1561 ccttctttct gtaagagtta tctatttgtt ttcttttttc tctatacact ggataagtca
     1621 ggcacagcca agaaagactg agtaagtagc cgccgatgat atcagtagga aaatgaacgc
     1681 ccagataaat gcggcttatc ccgacactta ctataataaa catcatcagc aattgaagca
     1741 atgtgcgtaa ggttcttttt tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa
     1801 gaatcatact gcctgctgca tgtccactag ggaaactgta acttgtttca actactaagt
     1861 gcgccaatga aggacgctct cgcatgaaaa atagtttgat caccgggttg atgattccag
     1921 caattacaat aaatccagaa acgaaccaaa atgtttctgc cctttttttc ttataccaca
     1981 tccataaagc cgcacaagca aataaaatga tgacggtaag aggattaccg aatttagtca
     2041 taaagagttg gtagctgttc agatcaggat aagaagctct gacaatgcct gtaaaccact
     2101 gatcgaaacc agaaagccac gacagatgga actttacgat ccagcccaaa atgaaaaaca
     2161 ggacaagaaa gaagctacca gccacctgaa atgatgtttt atatttcata caattcctcc
     2221 tttgaagcaa tgacttatta tagcattttt ggtccagcga aaaaaacggt aaagagataa
     2281 aaccaatgta tgattcctct ttgaataacc aata
//
LOCUS       DACEMO010000257         2260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.265, whole genome shotgun sequence.
ACCESSION   DACEMO010000257 DACEMO010000000
VERSION     DACEMO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.265"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            32..>2260
                     /locus_tag="IW728_002615"
     CDS             32..>2260
                     /locus_tag="IW728_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1025939.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAF"
BASE COUNT          702 a          404 c          498 g          656 t
ORIGIN      
        1 gaacccatta ctctggtaag gagtgtgaac tatgtacccg attaagtata ttgaaaataa
       61 tcttgtcttt aatcaagagg gagaatgttt tgcctactat gagttaatac cttacaatta
      121 ctcctttttg tcacctgaac agaaatatca agtccacgat aactttcgtc aattgattgc
      181 gcaaaatcgt gaaggcaaga ttcatgcctt acaaattgcc acagaaagta gtattcgtgc
      241 cacgcaagaa cgatcaaaaa aggaaattac cgggcgatta acagaagtag cgaaacaacg
      301 aattgattta cagacagagg cattagtatc catgattggc gatagtcaga ttgattatcg
      361 cttttttatt ggcttcaaac tgattgcgac agatgaagag gtcaatctaa agaatctcaa
      421 gaaatctttc ttctccggct ttcaagaatt tgtctatgga gtgaatcatc atttgatggg
      481 cgattttgtt tccttatcca atgaagaaat tcgccgatat accaagcttg aaaaactaat
      541 ggaaagtaaa ttggctcgaa gatttaaagt cagacgagtc acaccgagtg atctgatgta
      601 tttgattgaa catatctatg gcgaaaaggg tacaccattt gaagaatatg aatttcagct
      661 accaaagaaa aagctgaagt cggaaacttt ggtgaaacgt tacgacttat tacgtcccag
      721 tcgttgtttg attgaagaaa agccccggta cttatgcatg gaacatgaga atcatgaatc
      781 atatgtagcg tacttaacaa ttaatacaat tgtgggggaa atggagtttc cctcctcaga
      841 acttttctat taccagcaac aacaatttac attcccaatt gacactagta tgaatgtgga
      901 gattgtgacg aacaaaaagg cacttgctac cgttcgcaat aagaaaaagg aattaaaaga
      961 cttggacaac catgcctatc aatctgacaa tgaaacaaat tccaatgtct tagatgcatt
     1021 ggattccgta gatgaattgg aaactacctt ggatcagtcc aaagaatcca tgtacaaact
     1081 gagctatgtg gttcgagtaa gtgccgaatc agtagatgaa ctaaagcgcc ggtgtgatga
     1141 agtgctagat ttttatgatg ataccaatgt aaaactagtc cggccttttg gcgatatgat
     1201 ggggctacat gaagaattct taccttctag taagcgatac atgaatgatt atatccagta
     1261 tgttacgtct gattttctcg ccggacttgg ttttggcgcc actcagatgt taggggagtt
     1321 ggaaggcatt tactttggtt ataacgtaga tacggggcgc aatgtttacc tgaaaccggc
     1381 attggcctca caaggggtca agggttcagt taccaatgcg ctagcagcgg cattcctcgg
     1441 ttcactcggt ggcggaaagt cctttagtaa caatctctta gtttattacg ccgtcctttt
     1501 tggtggacaa gctgtgattg tcgatccaaa aggggaacgg ggtggctgga aagaaacctt
     1561 gccagaaatt gcggatgaaa tcaatattct caacttaact agtaagccaa aaaatcaagg
     1621 attacttgat ccttatgtga ttatggagaa aaagaaggat tctgaaagtc tagcaattga
     1681 tattctgacc tttttaacag gaatttctag tcgagatggg gagaagtttc cagtgttgcg
     1741 taaggccatt cggacggtta cccaaagcaa tcaacgggga ttattacaag taattaatga
     1801 gcttcgaaaa gaaggctcac cagttgcgga aaatatcgcg gatcacatcg agtcctttac
     1861 cgattatgat tttgcccatt tgcttttctc agatggttcg attacccagt cgattagttt
     1921 agataggcag ttaaatatca ttcaagtagc ggatttggtt ttaccagatg cagaaaccac
     1981 ttttgaagaa tataccacca tggaattgct tagtgtttcc atgctaatcg tcatttccac
     2041 ttttgccttg gactttatcc atagtaatcg agaaatcttc aaaattgtag acttagatga
     2101 ggcgtggagt tttcttcaag tagcgcaagg gaaaacatta tcgaataagc tcgttcgtgc
     2161 tgggcgggct atgaatgccg gggtttactt tgtcacgcaa aatagtgatg atttactgga
     2221 tgaacgcttg aagaataata tcgggttgaa gtttgcgttt
//
LOCUS       DACEMO010000258         2231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.266, whole genome shotgun sequence.
ACCESSION   DACEMO010000258 DACEMO010000000
VERSION     DACEMO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.266"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(199..1890)
                     /locus_tag="IW728_002616"
     CDS             complement(199..1890)
                     /locus_tag="IW728_002616"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1025940.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT          604 a          482 c          384 g          761 t
ORIGIN      
        1 agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac agtgtttaag
       61 agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg ctcaaaaaac
      121 gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc agaacgctga
      181 tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca aacgaaggtc
      241 ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt tttgttcatc
      301 atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta atttgattgc
      361 atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg tttcagggta
      421 tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat agtctgctgt
      481 ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat gtgtatattg
      541 cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa agtcaaatgt
      601 attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa cttgtttagc
      661 tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt tagcagattg
      721 gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag ttgataattt
      781 tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag accagtcaaa
      841 gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt cattccctac
      901 tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag cagctaagtc
      961 acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat tcaggtcata
     1021 agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt tttcttctaa
     1081 aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca acgaatcgaa
     1141 acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat cacggaacca
     1201 ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc aagcacgagc
     1261 ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt ataaacgaag
     1321 caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt tgtaagcgac
     1381 gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga ttggctgata
     1441 accgattttt tccataatga aaccgatcga attaccgata actgtcgaac gcaaatgtcc
     1501 cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg ttccttgatt
     1561 cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca aaactttttt
     1621 gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt cgattttttc
     1681 aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg gcgcttttcg
     1741 gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag ctgattttgg
     1801 attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag cgtcatagac
     1861 agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt tcccttgatt
     1921 gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta gagacgagat
     1981 ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga tatcggtaga
     2041 cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc ttcattcggc
     2101 ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg ttcaacaaat
     2161 cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg ctagtcgttt
     2221 cataaaaaaa a
//
LOCUS       DACEMO010000259         2222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.267, whole genome shotgun sequence.
ACCESSION   DACEMO010000259 DACEMO010000000
VERSION     DACEMO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.267"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..538
                     /locus_tag="IW728_002617"
     CDS             <1..538
                     /locus_tag="IW728_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1025941.1"
                     /translation="LTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLD
                     LENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGA
                     KIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNID
                     SVELREKVLRSIIDKSIE"
     gene            768..998
                     /locus_tag="IW728_002618"
     CDS             768..998
                     /locus_tag="IW728_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1025942.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1221..1319)
                     /locus_tag="IW728_002619"
                     /pseudo
     CDS             complement(1221..1319)
                     /locus_tag="IW728_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1584..2039)
                     /gene="tnpA"
                     /locus_tag="IW728_002620"
     CDS             complement(1584..2039)
                     /gene="tnpA"
                     /locus_tag="IW728_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1025943.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          693 a          388 c          353 g          788 t
ORIGIN      
        1 tttaacagaa gctctctatt ccactattag gcttttagac gaagaaaaat tgaatgaagc
       61 cattaaaatg atcactcaat caaaaaacat atatatattt ggagtgggat ctagtggaaa
      121 tactagtctt gatttagaaa atatgtttct tcgcgtagga gttcaagcaa aagctgtact
      181 agaccctcat tttcaagccc aagtcgcttc tttattaaca gttaatgatt tagtaatcat
      241 tttttcttta tctgggaaaa cgaaagatac ttatgattca ttgaaaatag ctaaaaaaaa
      301 tggcgcaaaa attattgcta taactaacta tattcattca cctataggaa agtctgcaga
      361 cttagtttta caaacagcca ttgaagaatt tcttaatggt ggatctttag ctggaaaaat
      421 atcacaactt tatatttgtg atctattagt tcatggttat gaacaaaaca ataatattga
      481 ttcagttgaa ttaagagaaa aagttttgcg ctctattatt gataaaagta ttgagtgaac
      541 tacccccact tagctaaacc taacggtttt taacgattga agtaggcaat tcttgggatg
      601 agagcatact tgatagctta ttttttacta atagcggtgg ctcttattga ccaaatatcc
      661 ctgtagttcc tacggttctt gattttatct aaagatattt ttatcgtgga agctattgat
      721 caacttgaaa gaaaaacgtg tagctcatta tcactagttt atcgagtatg gatgaagcaa
      781 tggatcatcc tctcttaaat aaatttatta aggatctgat ccttcaaatt ttttccatga
      841 tcgatgagca ggaacctact gaatctaaac gacatcgggc acaaggaatt attaagattg
      901 ctaagcgaat gttttctata aagggtgttc taagctatac agtgttaatt caaaagattc
      961 ccaatcatct tgtttataag aacaacgttg aagattaaaa aagtgtttca atgctaaaat
     1021 tgcaaaatac ttcaatgtta tgagacaaac tgtctataag aatcaatttc cacccatttc
     1081 atcacttgtt aaaagtttat gatccttttg atttttatat taatttatct gtatttaata
     1141 cagattttat ttattagttc atctgcctat tgtattttat tttgttgcct taccatctga
     1201 agtaaatata ctcttaattg tcaaattaag ttagacagat catcaatact tacgtaactc
     1261 aaaaatactt ccaaaggtgt tcgataatcc agtgattttc taggaagatt atttctgcgc
     1321 cagcattttc taatgtttga atttgtgttt tgtaatcttg gttgcttgtg ctaattcttg
     1381 aatagccgta aattgccaaa atttcatcac cctttctgga cataagtttc tgacaccagt
     1441 taaaatcaag tctatcagta acccatacac ccgtgaggac gggtgtattt ttaatccccc
     1501 tagaaggggt cgacactcgc tgccccttaa aaggggattt ttaaaagacc gccaactgct
     1561 catcttcact cacttacttc tctttatttt ttaaatggtt ctacgtactc tcttttactt
     1621 ataatatatc gaatgcgatc atcagcttct tgttcacgga tgtatttttc atttgtcttt
     1681 tgattcaatc cgactgtact cacatagtat ctcttcgacc aaaaggattt gtttccgtat
     1741 ttatatttga gatttgcgtg ctgctcgtgg attagtaccg cactacgacc tttcagatac
     1801 cccataaaac tggatataca tatttttggt ggtactcgaa caagcatgtg aatatgatct
     1861 agcattgcat gtgcttctat gatttccaca tctttcattt cgcacaactt cctaagtatt
     1921 tttccaagat cttttcgtaa ttgcccatag attacttttc gttgatattt tggtgtaaaa
     1981 ataatatgat acttacaatt ccaacgagta tatgctaaac ttttatcgtc atttgacatg
     2041 aaaaaactcc tttcgatctg atgagcggtt ggtagccact cactagtata tcgaaaggag
     2101 tttttatcgc taaagatact tcttccacgc gtagaaacgc gcggttttct tgtttcgcac
     2161 gctttacgca caaaactgac tgaagtcatt aagaaaataa aagggtctaa aacttttaag
     2221 tt
//
LOCUS       DACEMO010000260         2200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.268, whole genome shotgun sequence.
ACCESSION   DACEMO010000260 DACEMO010000000
VERSION     DACEMO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.268"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            15..2180
                     /gene="acm"
                     /locus_tag="IW728_002621"
     CDS             15..2180
                     /gene="acm"
                     /locus_tag="IW728_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1025944.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          866 a          348 c          425 g          561 t
ORIGIN      
        1 gggagataga aaaaatgaaa aagtgtagaa atattatttt atcaatgtta tttattatta
       61 ctaatataac atcactgatt cctgttcatg tctatgcgga tgcaggcaga gatatcagca
      121 gtaatgtcac ttcgttgacc gtggacccaa cgaatattac agatggagga aacataaaag
      181 taaaattctc gtttgacgaa aaaaaacaaa atatccaacc aggtgattat ctttggatca
      241 attggccaag tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga
      301 ttgaaaataa aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta
      361 atgaaaatat taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca
      421 gaaacgtaac cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata
      481 aaacagctac tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt
      541 ataaaacagg agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta
      601 acaataacgg cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac
      661 aaagattaga tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt
      721 atagaggaga agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt
      781 tttcagtaac tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga
      841 aaataatggt gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc
      901 attcggaagc ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc
      961 atacagttaa aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga
     1021 agattttcaa atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa
     1081 gacgagcaga tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac
     1141 agggagaagc tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag
     1201 cacccaattg gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg
     1261 aaaatgatac agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca
     1321 cagcaactaa aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat
     1381 tcagagcaag tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca
     1441 atggaataac ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat
     1501 atacgtttaa agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc
     1561 aaaaaattga aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag
     1621 gacaaaaaac atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag
     1681 taaatctatt agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt
     1741 ggcgctatcg tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg
     1801 taacggaaga caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca
     1861 gttatacacc ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa
     1921 attttccaga gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa
     1981 tatttgctca tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg
     2041 tacctaaaca gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat
     2101 ctagttttgc tgtaatcatt ggtttattga tagtaactat ctgtgctggg atattcttac
     2161 aaaaatataa aaaagtttag ttagaatata atattttttg
//
LOCUS       DACEMO010000261         2144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.269, whole genome shotgun sequence.
ACCESSION   DACEMO010000261 DACEMO010000000
VERSION     DACEMO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.269"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..306
                     /locus_tag="IW728_002622"
     CDS             <1..306
                     /locus_tag="IW728_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1025945.1"
                     /translation="LDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLD
                     SVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
     gene            306..1682
                     /locus_tag="IW728_002623"
     CDS             306..1682
                     /locus_tag="IW728_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1025946.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            1812..>2144
                     /locus_tag="IW728_002624"
     CDS             1812..>2144
                     /locus_tag="IW728_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1025947.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT          835 a          266 c          392 g          651 t
ORIGIN      
        1 ttagataaaa aacaaataaa aagaaatgtt atgaaattat ctggtggaca gcaacaaaga
       61 gtagctattg ctagagcatt ggtatcagat gctccaataa tactagctga tgagcctact
      121 ggtaatctag acagtgttac tgctggagaa ataattaata tattaaagac attagctaaa
      181 gatagaaata aatgtgtaat agttgtaaca catagtaagg aagtagcaga ttctgcggat
      241 atcattttag aactaagtgg taaaaagttg aaaaaagtaa ataaaatgaa tttggaggtt
      301 gaataatgat aaaaaatgca tttgcttatg taactagaaa aagtttaaaa tcattaatta
      361 tcatattagt tattttatct atgtcaactt taagtctcat tagtttatct attaaagatg
      421 ctacggacag agcttcaaaa gaaacatttg ctaatataac aaatagtttt tctatggaga
      481 taaatagaca agtaaatcca ggaacaccta gaggtggggg aaatgtaaaa ggtgaagata
      541 ttaaaaagat atctcaaaca gatagtatag actcctatgt aaaaagaata aacagtgttg
      601 cagatttagt tgattatgat attattgaaa ctcaagagac tcttgcgaat caatcacctg
      661 aaagagcaaa gaattttaaa agaacagtta tgttaactgg ggttaacgac tcaacaaaag
      721 aaacaaaatt tgtatcagaa gcatataaat tagtagaagg aaagcattta gaaaatgaag
      781 ataaaaataa aatcttaatg cataaagatt tagctgaaaa aaataacctt aaagttggag
      841 ataagataaa aataaaatct aatttatttg atgctgataa tgagaaaggt gcagatgaaa
      901 cagtagaagt agaaattaaa ggtctattcg atgggcataa tagcggtgta gtaagtgcag
      961 cacaagaact atacgaaaat acattaatca ctgatattca tacagctgct aaagtttatg
     1021 gtaataccga agatacagct gtataccaag atgcaacatt ctttgtaaaa ggtgataaga
     1081 atcttgatag tgtaataaaa gatcttggga aattagatat aaattggaga gaatataatt
     1141 tgattaaaag ttcatcaaat taccctgcat tacaacaatc tatatcaggt atttactcaa
     1201 tttcaaataa attatttttt ggctcattaa tatttgcagg agttgtagtt tcattattat
     1261 tattcttatg gatgaatgct agaaagaaag aaatagcagt attactatca ttaggtatat
     1321 caaaattaga aatttttggt caattcctaa ttgagatggt atttatatca atccccgcat
     1381 tcgttggttc ctatttctta gctcaatata cagctgacaa gcttgggaat aatatattga
     1441 ataaggttac tggtgatata gctaaacaaa tagctaaaca atctgcatct agccaattag
     1501 gtggtggggc agaagctgaa ggatttaaca agacattatc aagtttagat atcaatgtat
     1561 tacctaaatt catgatttat gtagttatat ttatgagttt agtacttctt gtatcattga
     1621 ttatttcttc attcaatata ttaagaagga atccaaaaga attattaatt gacaataatt
     1681 aaaattttcg gtgcttttta gcaccaaaaa tgagagtgta atataaaccg tgtaagtaac
     1741 catcaatcct aggcgggtgg ttgcttcacg gtttttattt ttctaaaata agatcaagag
     1801 gtgattacac gatggctaga aagaaaagaa atcctgatgc tgagaagtta gctgaatcca
     1861 ttttgaatac ttatcaaccc gaatctgtag aggatatgca agatgtttta aaagatgtgt
     1921 ttggtccctt atttgaaaaa atgcttcaag gagaattgaa taaccattta gggtatgacg
     1981 cccattctaa agagccaaag aaacatgaca atcgacgaaa cggttatggc aataagacgc
     2041 ttaaaacgag ctttggtaaa gtaaacattg atgttcccag agaccgggag gcttcttttg
     2101 aacctgagtt gattcctaat agaaaacgag atgtctccga catc
//
LOCUS       DACEMO010000262         2111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.270, whole genome shotgun sequence.
ACCESSION   DACEMO010000262 DACEMO010000000
VERSION     DACEMO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.270"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(314..589)
                     /locus_tag="IW728_002625"
     CDS             complement(314..589)
                     /locus_tag="IW728_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1025948.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            696..1460
                     /locus_tag="IW728_002626"
     CDS             696..1460
                     /locus_tag="IW728_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1025949.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSADQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1583..2086
                     /locus_tag="IW728_002627"
     CDS             1583..2086
                     /locus_tag="IW728_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1025950.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
BASE COUNT          668 a          395 c          453 g          595 t
ORIGIN      
        1 cttaatactc aaaactaagc gactttttca caactagtcc ttaatgcttg atgcagatca
       61 gctttttcgc aaccttatcg acggtgttca aaagcttgca tctacggcat taagtccagc
      121 ctgttgaaaa atatggagag ctatttttca caaccagtcc ttaatgcttg atgcagacca
      181 gctttttcac aaccttattg acggtgtttt atcagctgcc tctcggtatt aagtcattac
      241 atttcaaaat atgagaagct atttggaaaa tgtttctcct taatactcga ggcaaagcgc
      301 ttcaaaaaca accttatccc accacatcaa atttctttcg ttcctcaatc atcggatctt
      361 cttggacatc cacatactgc actcgtccac ttgggctcgg agacttcttg acttcttcga
      421 taaatttttg gatgatgtcg tcatttccga ttgcttcgat tgtgactgag ccgtcgtctt
      481 cgttttttac cgttccggtc acgcctaact gatcagcaac catcttggtc atatagcgaa
      541 atcccacgcc ctggacacgt ccttggacat tcattttaac tttgcgcatg ttcgtcacct
      601 ttctttcctg ttttataaat caatgatacc gattttttta ggatgaaaca agctcaagca
      661 cctattccat gatataatca gttgaggtga ggaatgtgaa agagatccaa tcagtaaaaa
      721 atccaagaat caaagaatta aaaaaactac ataaaaagaa atacagagaa gaaaaggcag
      781 aatatctttt ggaagggttc catctgatcg aagaagctgc gtcgtctgat gcagaaataa
      841 aagaactttt tgtgacacag agaggtatga aagaatgggg agactggctg gctgtaaaca
      901 aagagatttc tacttttcta gtttcagatg aggtcatgac cgctatgtca gatctaccta
      961 caccgcaagg gatcttagca gtgatgacgt taacatctga agatacgcca gatttttcag
     1021 gaggctggct tttactagat aatgtacagg atcctggaaa tgtgggaacg atgatccgta
     1081 cggcagatgc gtttggtatg gctggtgttt tattaggtaa aggaacggca gatatttata
     1141 gtacaaaggt gctgcgaagc atgcagggaa gcaattatca tttgcctgtg ttacaaaaag
     1201 atttggcaga agtgatccct gtaatgaaag gcaaagggtt tacggtgtac ggtacagaat
     1261 taaatgaaga agcacatgca ttagacaaag tagaaaaaac agaggatttc gctataatca
     1321 tgggaaacga aggtcaggga gtaagtcagg aaatcttgtc ttcggctgat cagaacgtat
     1381 atatcccaat gaaaggaaat gcggaatcat tgaatgtcgg agtcgcagca gggatcgttc
     1441 tttatcattt ttctttatga ttaaggaact attaagcttt agctatttta tctagagcga
     1501 ctagaataat ccgaacactg gtggtatact tagatcaatt tcagaagaaa atgaaattca
     1561 aaaaggtggc agatcgataa ttatgacaga aaaatggcgt gaagacgaag aatatatgtc
     1621 ttatgtacaa gatcttttag aaacagaagc agtgaaaaaa ttagcgaatt acacacagca
     1681 tgttcattcc actcgtttgg agcattccat cagtgtttct tactatagtt atctgctcgc
     1741 aaaaaaatgg ggtggcaatg ctaaagcaac agcgcgtgct ggacttttgc atgatctgtt
     1801 ctactatgat tggcgtacga cgaaatttga cgaggggaca catgcgtata tccatccaag
     1861 aattgctgta aagaatgcag aaaaaatcac cgatttgtcg gatcttgagc gagatatcat
     1921 tttgaagcac atgtggggtg caacgatcgc accgcctaag tataaagaag gatatattgt
     1981 gacattcgtg gacaaatact gcgcagtcaa agaagcggct cagcccatgt ctgcaagtat
     2041 gcgtaagaga tggcaacgtt actttgggaa agaatcctcg atttaacagg taaaaccaat
     2101 aatagaccat a
//
LOCUS       DACEMO010000263         2106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.271, whole genome shotgun sequence.
ACCESSION   DACEMO010000263 DACEMO010000000
VERSION     DACEMO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.271"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(71..997)
                     /gene="cysK"
                     /locus_tag="IW728_002628"
     CDS             complement(71..997)
                     /gene="cysK"
                     /locus_tag="IW728_002628"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1025951.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1137..2024)
                     /locus_tag="IW728_002629"
     CDS             complement(1137..2024)
                     /locus_tag="IW728_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1025952.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          583 a          471 c          355 g          697 t
ORIGIN      
        1 aaatttttgt gtgttctttt catctttaaa agttttttac ataaacctat ctggagaaaa
       61 accaagtgcc ttatatctct tggtaaaggg ctgtagataa ataacgttca ccattgtcgg
      121 gaaccacagc taataccttc ttgccttttc ctaattcttt tgcgactttt agcgcggcag
      181 caattgcagc gccagatgag atgcctacta aaatcccttc ttttcgccca acttctctag
      241 ctgtttccat cgcttcgtct ccactaatag aaaggacttt atcatacaca gaagtatcca
      301 gtgttttagg aacaaaacct gtaccgattc cttggatttt gtgaggtccc gcttctttcc
      361 cttctaaaat agcagattct gctggttcta ccccgattaa ttcgattttc ggatacacac
      421 gttttaattc tcggccagcg ccagtgatcg ttcctcctgt accgatacct gcaacaaacg
      481 cgtccaagcc atttactcca aatgcttgat gtatctcagg accagtcgtc ttctcatgca
      541 caagaggatt tgcttcattt tcaaattgta atggtaaaaa gtagccgttt tcttttgcca
      601 aacgttctgc ttctctgatt gatccagaaa taccatccgc accaggagtc aaaatcaact
      661 ctgcgccgta ccctttcatc aataaacgtc tttcgatact catcgtctca ggcatcacga
      721 tgattacttt atatcctttt gcaactccta ccatcgacaa tccgattcct gtatttccag
      781 aagtaggttc aatgatagta tcacctggtt tcagtagacc atcgtgttct gctttttcta
      841 tcatacttaa agcaatacga tcttttacac ttcctcctgg attaaaaaac tcaagcttta
      901 caaaaacatc tgctgaatct tctggtacga ttttatttaa tttgacgatt ggcgttttgc
      961 caatcaattc ggtgactgag ttatagatag ctgtcatctt caattcctcc ttgtctttgc
     1021 tcttcttact tatcataata caatctgttc atttatggcg agtgattcgt ctttgactag
     1081 caaaaaaaag acaaagatat accatatatg gtaatttctt tgccttttta tctaaatcaa
     1141 acgtttagtt cttgaccaac taataaagaa taactggaca tagacatgcc atttttcgcc
     1201 attagatcat caacagacat gccaaatttt tgtgcaatat cccatagcgt atcacctgac
     1261 acgactgtat atgtctgacc attatttgta tttacatctg ttgttgaaga tgatgacgtt
     1321 gaagtcgtcg tagtagtcgt tgttgttgta cttgtgattg ttgtagccga acctgttgat
     1381 ggcgtatcaa aacgggttaa attataagta ttaatcaatg agttcaattt ggccccataa
     1441 tttggatcgg tagcataacg acctgtcaag tacgctgttg catcataaaa acttgttgta
     1501 ttgcttttcc acacgccagc ataatgataa tcgcccgtgg aaaaactggt tgaacgaaga
     1561 acattcgcat ggtcttggaa ggattcccaa taagaagaat attttcggaa tggctcattc
     1621 atcgttaccc attgtccgtt tacatattct ttggttgcca tataaacgac ctgtccgtta
     1681 tagcttcctt tcacaccgaa tagattataa tttggcgcac tagctaatcc ggaagtgcca
     1741 tatgcacttt ccaacaacgc ttgagcgatc atgactgaag catacaagtc attacttgca
     1801 gcaacttctg acgcagacca gccgatttga tcgataaacg cttgaggatc agcgactgtt
     1861 tgagatcctt gttcatctgc atgaacatgt tgcggtaccg ctgtaaccat catcggccca
     1921 actaaaagac tcgttccaat taaaggagca atcgcttttt tattgatcac catttttctt
     1981 ttaggtctat ggtgccgggc actgcgggtc ttcagctctt gcattcaaat tcctcctcaa
     2041 aataaaaaaa cttaataaaa agttctcttc ttaaaacaaa cttaattaat taaattatac
     2101 ttttaa
//
LOCUS       DACEMO010000264         2090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.272, whole genome shotgun sequence.
ACCESSION   DACEMO010000264 DACEMO010000000
VERSION     DACEMO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.272"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            111..1412
                     /locus_tag="IW728_002630"
     CDS             111..1412
                     /locus_tag="IW728_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1025953.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(1481..2080)
                     /locus_tag="IW728_002631"
     CDS             complement(1481..2080)
                     /locus_tag="IW728_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1025954.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT          677 a          407 c          362 g          644 t
ORIGIN      
        1 tattttttct gcaatttatc gagaagtcac tttactttgc ctcttattct ttcttcgtta
       61 caataaggaa tgggacaagt aaaaaaatga aaaagcaagg gacgaggtat atggaaaaaa
      121 agcaaaaaat tattttagga gccacttctg ctttaactat cgttatttta attctaagtt
      181 tgttttttgt catccgatca tctacaaaaa aagaaccaat cattcctgaa aaaaaaataa
      241 cagaaaaagc aaaagaaccg acagatgata agcaacaact agttactcaa gcaaaaaatc
      301 tggctcaaag ctacgattat gaccaagcga ttgcgttatt ggaaaaagat agtaagaacg
      361 gatctactga aacacaacag cttcttcaaa cactaaaaaa ggagaaagaa caactgatcg
      421 actggtctga tcctactcag atctctcatg tctttttcca cagcttgatc gtggacccgc
      481 aaaaagcctt ccactctcaa caggcacaag gatacaaaga ttatatggtg actgttgaag
      541 aattcaaccg ttcgatcgaa caactttatc aaaatgattt tgttttagtt aatttgaatg
      601 atttgatcca aaaagacgaa caaggaaatc tttcctttaa cgggcttgcg ctacctgaag
      661 gaaaaaaacc attggttctt tcacaagatg atgtcagcta ttatgaatat atggataatt
      721 ccggttttcc tagccgattg gtcgtatcaa ataacaatga aatcaaaaat gtctataaag
      781 agaaagataa agaagaaatc ggtgactttg acatggttcc tctgatcgat gcctttgtca
      841 agaaacatcc tgatttttct tatcgaggcg caaaaggtac cttggccctg acaggttaca
      901 acggagtttt aggttatcga acatcaaaat ctcaatatgg agacaatgaa aaaacaaaaa
      961 aagagattaa agaagctaaa aaagtggcgg aacagttgaa gaaagacggc tggagctttg
     1021 cttcccatac atggggtcac ttgaatatga ctcaagcttc actagaagat gtaaaaaaag
     1081 acaatgaatt gtggcagcaa gaagttgcac ctatcctagg aaaaacaaat attctgattt
     1141 atccatttgg tgcagacatc agcgactggc agccttattc agcagaaaat caaaaatttg
     1201 cttacttgaa gagtcaaggt ttcgatattt tctgtaatgt cgatgcttct acaccagctt
     1261 ggggacaatt aggtacggat ttctatcgaa atgctcggat caatatcgat ggcattcgtt
     1321 ttcaatctga tttagacgga aaaaatccta ttcttgatcc attcatcaat gtccatgatg
     1381 tctatgacaa agaagcacgc gaatctgctt gattttacgt catttatgaa ccaaaaaagc
     1441 cgaactgaca ttttatcgtc agtttcggct tttttatgct ttatttcttc ttcggtgcga
     1501 ttcccttgat caggacttct gtccattctg ctgtatatgg aagcagtttt gcgtatatct
     1561 ccggcttaga aattccttct accattgctt gaaaatagag gatcaagaac tcatccgaat
     1621 atttcaaatc cacttttcct tctttccgcc ctcgatggaa aagatcaagc atgatactga
     1681 aactttcttg cgtatacgca tccatcgcgt cagaaaattc ttttttttct ttttttgtat
     1741 agtatgtcat caaatcgata taaaattgtt tgttcactcg attgagataa cggattttgt
     1801 tttcactcat ggcaatcagt gtttcttcaa atggaagatt ttccgcaatc aacgctttcg
     1861 cttcttgccc taaatcattc ataaatgcaa aaaatacttc ataaatcagc ccttccttgc
     1921 tgccaaaata tttaaaaatc gtcgctttac ttacctgagc gttctcagct accttatcca
     1981 ttgtcactcc agcggttcct atgtctgtgt tcatcaagtc aaaagctgct tgtaaaatct
     2041 ctttcttttt ctcttgtgct ctcttttcaa aaccgttcat ttgtagagcc
//
LOCUS       DACEMO010000265         2087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.273, whole genome shotgun sequence.
ACCESSION   DACEMO010000265 DACEMO010000000
VERSION     DACEMO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.273"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(325..1290)
                     /locus_tag="IW728_002632"
     CDS             complement(325..1290)
                     /locus_tag="IW728_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1025955.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
     gene            complement(1852..2055)
                     /locus_tag="IW728_002633"
     CDS             complement(1852..2055)
                     /locus_tag="IW728_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025956.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
BASE COUNT          600 a          480 c          329 g          678 t
ORIGIN      
        1 taaacattga cgtaaattta ctctcactta tctgactttt ttactcggta tcactccgag
       61 taagccgtca tattcattgg gataatctcg tctgcttcgc tctaattttc cagagtgagg
      121 ggtaactcct tcccccgaat taccccaaag agtgacactg ggatatccct aaatttatta
      181 taaaaagcca agtctctttg cgcaactgtt ttatccaagt cggcatttgc ggattcgtta
      241 gcgttttttc gctcttgctg caaacgcacg acccaccatg ccgacttgtg ctgaaaataa
      301 aaaagtggac aaaaattcat ccactcattc ttccgtcacg tccaacccca aaagccacgt
      361 atccaattct tttttcaaaa catctgccga ttccgaaaat gtctctcgta aatgattaac
      421 taactcatca ctttggcttt cttttttcaa caaatttcga tatttatatt ttgtatgacg
      481 agccaagcca gcccataaat tttgatctga aatcaatcct gcaatcataa ttctatcagt
      541 atgctttgta tcagtcggca aaaaatgagg acttgcgaaa ctgtctaaac tttcatgaac
      601 catcgcatac caatcagtag cttttccacg ccgtaactgc aattccagtc gccaccaagt
      661 ttttatttcc ggtggtacaa ttttccgttt ggtttcttgt tccaattttt tgttgtacat
      721 ccttatttgt cgctcactgg cacgactacc ccaatatgcc gtttccagct tcccagaccg
      781 cccatagatt ggtttgaatg aaacagggtc aaccacacga tattgcgtaa taaaatcatc
      841 aggcacatcg ataatgtcgc aagcaatatc agcacgtgag aaatgtggtt ctaaaaataa
      901 atcatggata aagttcttca tcgaacccgc caaaaattgg ctaattttat ttgggttaaa
      961 gtcaattcgc ccttgccctt cttgatattt caaaatttca atgtaagcaa tattctcatc
     1021 atgcttatcc acaatttgcc aaccatttag accaacagat ttaacataac cattattttc
     1081 attcagtcgc attaactgtt caaagtctaa aatcaataca tcattttgcg tgtgataata
     1141 aatattctct ttgagttttc caacaatggt aattcggtca attgaccact gtattttaag
     1201 gggggtggtc aaagtttgca cacttaaccg cctgttagat agggctgtta aattttccca
     1261 ctcatttatg atcgtgccac gtttactcaa attttgttcc tcctcaaaga aaaagagact
     1321 tttggaaaga atttccagag ttcacgtaac cctaaaaatt catatccaaa aatctctagc
     1381 ttgaagaaac ctaaaaatat tcgtataatg aacctgagtt acggagctag gtgaagtagt
     1441 ttccgtaaat agtcgagagt gttagcgcac tgctcggctt ttattttgct cttttttctc
     1501 gttttctttg attatagcac tcctcctaaa aaaataaaaa gacctcaggg aatttcattc
     1561 cccgaacccc ttgagcctcc tcctgccgaa atggaaaggg cgcacccgct gtttatttca
     1621 ttcagctatc ggctttcaca atctaacacg atcgttccgc taccagtcgc cttacaggct
     1681 ccattttcgc tcctctaacg tgttttaacg attgtgccga taactaaacg aaataaacgc
     1741 taaaaccgct cagaacgcaa aatatgccat tttagagcta ttgtagaaaa tgtagtttga
     1801 aatgcaattt tcttctttca gctttttatt tcccacttgt aaatctgcta attattttct
     1861 accagacaaa ccattgattt tttggctatc agttaactca cgattattat gagaaatgct
     1921 tgtattcttt gttccctttt tgaacgaaat actaaaccct aaggtgaacc cctccttcgc
     1981 gctgtcgcta ataaaaagag tgtaaacctt tctgttactc tttgacaaag agtaacattt
     2041 tcctaattgt gacatggctg tcacaattcc aaacgctctc cgagggt
//
LOCUS       DACEMO010000266         2064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.274, whole genome shotgun sequence.
ACCESSION   DACEMO010000266 DACEMO010000000
VERSION     DACEMO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.274"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..894
                     /locus_tag="IW728_002634"
     CDS             22..894
                     /locus_tag="IW728_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1025957.1"
                     /translation="MVQRSRQTIYNVYRFLKSGGSAISYYEQYKKNKRNCGRRPIVLP
                     DGQQEYIQKKVAQGWTPDVLIGRAEFPIACSVRTLYRMFKDKTFDPHDLPMKGKRKPN
                     GHKEKRGKQAFRRSIQDRTTDYIQFNHEFGHLEGDTIVGGKHKSAVITLVERLSKVII
                     TLKPAGRQAIDIENRLNQWFQSVPKHLFKSITFDCGKEFSNWKKISNTNDISIYFADP
                     GTPSQRGLNEHSNGLLRKDGLYKAMDFNKVSETFIQSVASKRNHIPRKSLHYRTPLEV
                     FLSYVGKDDLSSLI"
     gene            complement(881..1486)
                     /locus_tag="IW728_002635"
     CDS             complement(881..1486)
                     /locus_tag="IW728_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025958.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(1726..>2064)
                     /gene="tnpB"
                     /locus_tag="IW728_002636"
     CDS             complement(1726..>2064)
                     /gene="tnpB"
                     /locus_tag="IW728_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1025959.1"
                     /translation="IKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKAD
                     WYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINILTE
                     GLRLHSMGLA"
BASE COUNT          668 a          368 c          338 g          690 t
ORIGIN      
        1 tctgtttcaa aagtagccca tctggtacaa cgttctcgac aaaccattta taatgtctat
       61 cgttttctta aatctggtgg aagcgcaata agctactatg aacaatataa gaaaaacaaa
      121 cgtaactgcg gaagacgtcc gattgtgtta ccagatgggc aacaagaata cattcaaaaa
      181 aaggtggctc aagggtggac tccagatgtc cttattggtc gagcagagtt tcctattgct
      241 tgttcggtac gtacactata tcggatgttt aaagataaaa cctttgaccc tcacgattta
      301 ccaatgaaag ggaaaagaaa accaaacgga cataaagaaa aaagaggaaa acaagctttt
      361 cgccgttcga ttcaggatag aacaactgat tatatacagt tcaatcatga gtttggtcac
      421 ctagaaggtg acaccattgt cggtggaaaa cataaaagtg cggtgatcac gttagttgag
      481 cgattatcta aagtgatcat caccttgaaa cccgctggta ggcaagctat agatattgag
      541 aatagactga atcaatggtt tcaatctgta ccgaaacact tgtttaaatc catcacattt
      601 gattgtggaa aagagttctc gaattggaaa aagattagta ataccaatga tatttcgatt
      661 tatttcgctg atccaggaac accttctcaa cgaggactaa atgaacattc gaatggctta
      721 ttacgtaaag atggcttata taaagctatg gattttaata aggtatccga aacctttatc
      781 caatcggtgg catcaaaacg aaatcatatt cctagaaaat cattacacta tcgcacacca
      841 ttggaagtat ttttgagtta cgtaggtaaa gatgatttgt ctagcttaat ttgacaaacg
      901 aaactttgta aaaatattcg attgaatatt ctatttggca cgactcactt ggcagtatat
      961 gtatgtcaat atttttcatc tgtttcatag gtatttcaaa atctaaacta ttaaaaacat
     1021 ataattccct gtaattattt acatctaact ctactaaatt tatctttgga acaatagatc
     1081 ctccacccat tccaccataa taaaaaaaat cttgttctct atcaaaatag cacatattaa
     1141 gctcagtttg tttatcatcc tcaataattt tataaatcaa acctatataa tcatgaatgc
     1201 tggcaaaaat tttttttaag tcaagttcta aatcttcttc gaaaacacat ttaatttctc
     1261 ctactgataa ttgatactct atattacatg acttaattag ttttttagat cccccctcca
     1321 gataggcata tgtttcaata attatgttct ttttattgct tggcaaattt ccgttattta
     1381 ttgcataaat taaaaatgac tgtctattta gcccataatc taatacaact tcttcatact
     1441 catatttctt taaatattta atatgaaaat atgctgaatt aatcaaaact cgagtgttac
     1501 tgttgttatt tattgttgtt ctattgtgta taaaattgta taggttgtca tattttcttg
     1561 gtttagtgaa ctactcacca cttagctaaa cctaacggtt tcttacgttt gaagtgggag
     1621 cttcttggga agagagcata cttgatagcg tattacttta ctaacagagg tgcctcttat
     1681 ttaccaagct atccccgtag ttcctacggt tcttgtttat ttgatctaag ccaatcccat
     1741 agagtgtagc cttagacctt cggtcaggat attgatacta gcgttgatat ctcggtcatg
     1801 atgagtatga caaatagggc aagtccattt tcgaatttcg agagatttct tgccgtcttt
     1861 atgtccacaa ttcgaacaga tttggctaga tggaaaccac ttatctactt tgatgatttt
     1921 ccgaccgtac cagtcagcct tgtattgtaa tttagacaca aaactagacc aagaaacatc
     1981 cgaaatactt tttgctagtt tatgattacg caacatgcct tttgtgttta agtcttcaat
     2041 acagatgata tcgtgatttt tgat
//
LOCUS       DACEMO010000267         2038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.275, whole genome shotgun sequence.
ACCESSION   DACEMO010000267 DACEMO010000000
VERSION     DACEMO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.275"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            272..865
                     /locus_tag="IW728_002637"
     CDS             272..865
                     /locus_tag="IW728_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ1025960.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            878..1777
                     /locus_tag="IW728_002638"
     CDS             878..1777
                     /locus_tag="IW728_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1025961.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            1780..>2038
                     /gene="ssb"
                     /locus_tag="IW728_002639"
     CDS             1780..>2038
                     /gene="ssb"
                     /locus_tag="IW728_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1025962.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQ"
BASE COUNT          808 a          311 c          399 g          520 t
ORIGIN      
        1 taagaaaaga acacgccgca ctacaaaata atgaaggggt tatcctatgc gaaatacaac
       61 acacaaacaa caagaaatcg aaaatcaaaa agcaaaaatt cgtttagaac aagagaaatt
      121 acgtgcaaat aaaagaagac tgcaacaatt aaagaaccag cagcgcattc aaaaagaaat
      181 cgaattagat cgatagacgt taatcttgtt acaaataaac tactatgata tatttgatgt
      241 agtgtatttg taacgaaggg aggggaagta aatgcaagat aaactgaaag aattgttggc
      301 tattcgcgat ggaaatttat ctatgaagga agccaggaaa tttggaatcg cagcaaccac
      361 tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt gatcgtggtt attatgtaga
      421 ggatggtcat ggaatagatg atttattttg gcttcagcaa cgattttcca ggggaatctt
      481 ctctcatgaa acgactttag atatttacca gctttctacg aatatgccga aattcattca
      541 cttaactttt ccccatggct ataacgttga tcgttcaatt acaaaagagc agcagttaca
      601 atttcatttc gtgaagaaag aagtttatga actaggaaaa gtagagggaa cttcctttca
      661 aggaaatcct attactatgt atgataaaga gcgtacactt tgtgatattt ggaatccgag
      721 atatgaattt gaatatgaaa tgaaattaga agcattaaaa gagtacatgg aagacccatt
      781 gagagatcca agtaaattaa gagattacat gacaaaacta catgtagaaa aagaaatgaa
      841 ataccacatg caaagcttat attgatggag gaaaaagatg acaccgacac aatttgcagc
      901 aaaaacaaga aatttagcaa agagtacagg attaaatgca cagttagtgc aacggcattt
      961 tatgatggat cgtctaattg aacaaatttc tgaatcaaag tacaaaaatg acttcatttt
     1021 gaaaggcggc tttttattag gatcaaaata tggaatcgat agaaggacaa caattgatat
     1081 cgatacgaca tttagaaata gcaaattaac tgaagaaaaa ttgaagagca tttttaatga
     1141 tttaactaga aaacccacaa aagaaggcat tgttttctct attcaaggaa tgaaggaaac
     1201 aagagaagcc gatttttatc ctggatttca aattaaattg gtggctaatt tagagaaagt
     1261 acgtgtaccg tttaaaatgg acgtgacaac aggagattct atttatcctg aagtagatac
     1321 acactatcac caattaatgt ttgaagataa aaaagtggag attccagctt atccaacaga
     1381 acaaattatt gcagaaaagc taagtgctac gttcagcttt ggcacagata attcgagagc
     1441 aaaagattac tacgacttat ttaccattcc gaaactagaa aatatcgata cgaaagagtt
     1501 atacaaaagt gttcgaaata catttacaaa acgtggggat acaaaaccgt tgtcctttta
     1561 ttatgaaaga gaaatggata agatcagagg aaatgactat ttggaaaagc agtgggaaaa
     1621 atatcgctca gttaacacgt ttgcgaattc catttcattt acagatacag tcaacgaaat
     1681 tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa gaaagaaaac ggcagtttaa
     1741 aagatcacaa caaatgaatc aagaaatgga gagataaaga tgattaataa tgtgacatta
     1801 gtaggacgct taaccaaaga tcccgattta cgttatactg caagtggcac agcagtagcg
     1861 acttttaccc tagccgtcaa ccgaaatttc gcgaatcaaa acggcaatcg agaagccgat
     1921 tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa
     1981 ggcggcttga tcggtgttgt cggacggatt caaacacgaa cttatgacaa ccagcaag
//
LOCUS       DACEMO010000268         2037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.276, whole genome shotgun sequence.
ACCESSION   DACEMO010000268 DACEMO010000000
VERSION     DACEMO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.276"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..425)
                     /locus_tag="IW728_002640"
     CDS             complement(<1..425)
                     /locus_tag="IW728_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1025963.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="IW728_002641"
     CDS             complement(534..1565)
                     /locus_tag="IW728_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1025964.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          627 a          331 c          351 g          728 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gcaattatat tgagaattaa tcctttttta tataagttgt ttgctta
//
LOCUS       DACEMO010000269         1971 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.277, whole genome shotgun sequence.
ACCESSION   DACEMO010000269 DACEMO010000000
VERSION     DACEMO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.277"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            223..789
                     /gene="amaP"
                     /locus_tag="IW728_002642"
     CDS             223..789
                     /gene="amaP"
                     /locus_tag="IW728_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1025965.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            802..990
                     /locus_tag="IW728_002643"
     CDS             802..990
                     /locus_tag="IW728_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1025966.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1003..1560
                     /locus_tag="IW728_002644"
     CDS             1003..1560
                     /locus_tag="IW728_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1025967.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1592..1834
                     /locus_tag="IW728_002645"
     CDS             1592..1834
                     /locus_tag="IW728_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1025968.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          722 a          304 c          402 g          543 t
ORIGIN      
        1 atcttgaata atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag
       61 caattgttat ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg
      121 tagcgcttta tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta
      181 gagttattta cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa
      241 ataatcggtg ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc
      301 aatgcgccgt atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat
      361 tactacatgc aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc
      421 atcttgctag tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa
      481 gatggcaaac taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac
      541 gaagaagagt tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat
      601 atccatgtca acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg
      661 atgcaagata tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg
      721 gcagtcacat acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga
      781 gtggaatagg agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc
      841 tttgggggtc ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca
      901 ttgttgatca taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca
      961 ggaatagttg atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg
     1021 aatacaacaa atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt
     1081 gaattgactt atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta
     1141 gatggtctat tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac
     1201 acagataacg taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat
     1261 ctagatgtga tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa
     1321 ttgatcagta ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat
     1381 gttgcagaca tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa
     1441 gtaacagaag cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa
     1501 tctgctgtag gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa
     1561 gaagtaagaa gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg
     1621 cggtatcatt ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat
     1681 cgcaaacatc attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg
     1741 gggaccaaca attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt
     1801 attagtcgtt tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga
     1861 aatgaagaac aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt
     1921 atatgaagta ttctttattc cttatgaaaa aatatcttta aaaataaaaa a
//
LOCUS       DACEMO010000270         1951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.278, whole genome shotgun sequence.
ACCESSION   DACEMO010000270 DACEMO010000000
VERSION     DACEMO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.278"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..770)
                     /locus_tag="IW728_002646"
     CDS             complement(<1..770)
                     /locus_tag="IW728_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1025969.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(763..1698)
                     /locus_tag="IW728_002647"
     CDS             complement(763..1698)
                     /locus_tag="IW728_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1025970.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
BASE COUNT          610 a          444 c          334 g          563 t
ORIGIN      
        1 taacttctca atcctcttcc tttattgatc aaataccaag taacggcgta caaaacaacg
       61 atgaataaca caagtacgat catcgatgtt gctactggca catcgatcgt tccaaggaat
      121 ccgtaacgga aaccggagat catataaacg atcggattga cttttgaaac agcttgccaa
      181 aagggcggta acatagagat ggcatagaaa acgccaccta gataagttaa cggctgcaag
      241 acaaatgtag gcacgatcga tacatcatca taagactgag caaaaatccc gttgagcagt
      301 cctgctaatg aaaacaagat tgccgtcatc aataatgtaa tgatgaccat gaaccatgaa
      361 tacacatgaa gtggtacaaa gaacaaggaa atgatcgtta cgagagagcc gacgagaata
      421 ctgcgaccca caccaccaat cacaaagccc caaataatca catgcgtagg aacaggtgct
      481 accaagattt cttcgatatt tttctgaaat ttttgagaga agaaggacga agaaacattg
      541 gcatacgaac tagtgatagc agacatcata atcaaaccag gaacgatgaa ttccatatat
      601 gaaaaaccac ccatgtcacc gatccgccca ccgatcattt ttccgaaaat aacaaaatac
      661 aaggatgtcg tgatcactgg cggaaccagt gtttggaccc atatacgcaa atagcggttc
      721 gtttccttga tagctaaact ttttaatgca gtaaaataaa gattaaacat ttttctcctc
      781 cgtatgatta ttttcttctg tgattttcaa gaacagttct tctaaacgat tcgacttatt
      841 cctcatagaa agaactttga ttccttgtct tgtaagctga tcgaagattc cgttcacccc
      901 ttgatttcgt tcaacttcga cagcgattgt ctgctcatct tcaaatgtac aagcatatcc
      961 ttcaatcaca ggtttggtat catatggcgc taaatcaaag ataaatgttt caaattgcag
     1021 ttttgacaga agctctttca tacttgtatt ctcgatcagt tcacccgatt ggatgattcc
     1081 gatgttgcga caaagcattt cagcttcttc tagataatgc gtcgtcaaaa tgatcgttgt
     1141 accattttcg ttcaattctc gtagaaactc ccacatctcg cgtctcaact cgatgtcaac
     1201 tcctgctgtt ggttcatcca agatcagcaa acgtggttca tgcatcaatg ccctagcaat
     1261 catcagtctt cgcttcattc ccccggaaag catacgtgca cggacatttc tcttttccca
     1321 aagattggac tgcttcaaat atttttcgct gcgtttcatc gcttctttgc gagagacacc
     1381 gtagtagcct gcttgattca cgacgatttg ctggaccgtt tcaaaagggt taaaattgaa
     1441 ctcttgtgga accaatccaa tctgttgctt tgctcgaact agatccttgt ccagatcata
     1501 accgaagaca ttcacttttc cagatgtttt attgactaaa gaagtaataa tcccgatcgt
     1561 tgtagacttc ccagctccat ttggccctaa tagcgcatag aaatctcctt ctttcaccga
     1621 aagatcgatt ccccgcaatg cttcgacccc acttccatag atctttttca aattatttat
     1681 ttcaagcgca tttgccattt atttcctatc tccttataga catgattctt aattgtatca
     1741 tggaatacag cagatgaaaa aacaacttgg cttccacaca aaaacacacc tgctaacaaa
     1801 gaggagatgt gttttctagt tcatgatcat cttcgataga ataactatct aaacaactct
     1861 ggaactttta aaatcataaa agtaccgaca gcaactacta cgatgacata tagaagatcc
     1921 ttataactga ggttcttgtc ttttttattc a
//
LOCUS       DACEMO010000271         1946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.279, whole genome shotgun sequence.
ACCESSION   DACEMO010000271 DACEMO010000000
VERSION     DACEMO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.279"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..504
                     /locus_tag="IW728_002648"
     CDS             <1..504
                     /locus_tag="IW728_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1025971.1"
                     /translation="INELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            494..1636
                     /locus_tag="IW728_002649"
     CDS             494..1636
                     /locus_tag="IW728_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ1025972.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1649..>1946
                     /locus_tag="IW728_002650"
     CDS             1649..>1946
                     /locus_tag="IW728_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1025973.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYY"
BASE COUNT          689 a          256 c          400 g          601 t
ORIGIN      
        1 ataaacgaat tgaatagctg ggcggatagc aaaaaaaaaa tctataaatt tattgtagct
       61 attggtgact gtgtagtaag aaaaaagatt ataaaaaaat tgaatatgcc aagtgagcaa
      121 tatggaatag ctgttcatcc aagtgctata gtggcaaaag aaatttcaat tggttatgga
      181 actgttattt ttgcgaatgc tgtaattaac ccagatgctg taattggaga gcatgctata
      241 ataaacacag gtagcattgt tgagcatgat tgtagaatta ataactatgt tcatttatct
      301 ccaggtgtat gcttagcggg tggagtacat gtaggtgtag gaactcaaat aggaattggt
      361 tctcaatgta tccagatgaa gaaaattgga tcttggtgta tgataggcgc agggagcact
      421 attgttaagg atattccctc acattcctta gcttatggca atccagctaa aattaagaaa
      481 gaaggaatta attttgaatt ctagaatttt attatcaagt cctcatatgg gaggaacaga
      541 agaaaaatat gttgctaagg cttttaaaga taattggata gcgccattag gtgaaaatgt
      601 ggataaattt gaagaaagta ttacaaaata tactggttct aagggcggag cttctgcgtt
      661 aagttcaggt acagctgcca tacatcttgc cttaagactg cttaatgttg gaaaaaacga
      721 tattgtcttt gctccttcat ttacttttat agctacagta aatcctattt tatatcaagg
      781 ggctgtacca gtgttcattg actcagaacc taatacatgg aatatgagcc ctgttgcact
      841 agagagcgca ttagaagaag ctgaaagaaa caaccaatta cctaaggcaa tcatcgtagt
      901 gaatttatat ggtcaaatgg ctgattatga acgaatttta gctattgcaa accaatttaa
      961 tgttccagtg atagaagatg cagcggaatc gttaggttct acttataagg gaatttcgtc
     1021 aggcttattt gggacaattg ggatctattc atttaacggt aataagatca tcacaacctc
     1081 aggtggagga gctctagtgt ctagtgataa aaaatatgtt gagcattcaa gatttttagc
     1141 aacacaagca agagatcaag caccgtatta tcaacatagc gaaataggtt ataattaccg
     1201 tctaagcaat atttctgccg gtatcggacg tggacaaatg gaagtattga atgatagagt
     1261 agctaaacga agagaaattt tcaatcgata taaggaagct ttttctggaa ttgatagaat
     1321 tgaaatgatg ccagaagtgg atggcatggt gccaaatcgt tggttgtcta ctttaacgat
     1381 caatgattat aattctattg atcaagtagt taacgtaata aataaactaa atgaaaaaaa
     1441 cattgaaaca agagctttgt ggaaaccact acatttgcaa ccagtatttc aaggggtaaa
     1501 aatgtattac catgataata atcttagtgt gtcagaaaag ttatttaata gcggcttatg
     1561 cttaccttct ggaagtaata tgacagatag tgaacaagaa tttgttattg atgagtttaa
     1621 aaaaatattc aagtaggtgt gatattttat gaacatagga attattggtt taggttccat
     1681 ggggaaaaga agacttagac tcattcaaga acactttaat gatattttac tatgtggtgt
     1741 tgatactaat gaggcgagac gaaaggaagt tgaaaaggaa tttggaatac caacttataa
     1801 tagtctaaca gaggcaaaaa atttgtttga aataaattca gtttttgttt gtacatcccc
     1861 tataacccat gaatttatta taaaagaggc tttaaattta gattgtaata tctttacgga
     1921 aatcaattta ttaaatgaat attatg
//
LOCUS       DACEMO010000272         1938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.280, whole genome shotgun sequence.
ACCESSION   DACEMO010000272 DACEMO010000000
VERSION     DACEMO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.280"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..1084
                     /locus_tag="IW728_002651"
     CDS             2..1084
                     /locus_tag="IW728_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1025974.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..>1938)
                     /locus_tag="IW728_002652"
     CDS             complement(1305..>1938)
                     /locus_tag="IW728_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1025975.1"
                     /translation="GKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFV
                     TGVRSEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTV
                     EDLKTWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSH
                     VAFLIEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          664 a          347 c          336 g          591 t
ORIGIN      
        1 tatcaatgat gatggctcaa gaaaaaaagt ggagaaagtt ggcggagaca cccgaccaga
       61 ggccgaagct gctttacgaa aagttttatc agatattgac gaaacaggac aatacttttt
      121 aggtacggat actcgagtaa aacaatacct tgatttttgg atggaggaat acgttaaact
      181 aaatctaaaa tacaatacct atgaaaacta ccgatttacc atcaaaaatc atataaacgg
      241 ttatttagga aagaaaaaac ttacggatct ctcccctgct cttttacaaa atttcatcaa
      301 tgctgaattt aaaaagggtt actcgaagaa aacaatgact attactcact ctgtccttaa
      361 gaatgcgctg aatatggcgg tttatccttg gggattaatc aagcaaaatc ctatgctgta
      421 tgtaaaaata ccaaaatatg aagcacgacc aacgactaaa aaagatctaa aaatcatttc
      481 tcttgaggac tttgatcata tgctagaaat tactcctgaa ggacatcctt tctatattcc
      541 tttgaatatt ggattttata cgggaatgcg cgttggcgaa gtttgtggtc tgacgtggga
      601 tgatgtcgat ttttcaaatg gaacaattac tgtagagaaa caaatggtaa agaatgacgg
      661 cgcatgggta tatggtacgc caaagacaag cagttccaat cgaacgattt ttattggaca
      721 aaccttgcta gcaattctga aaaaacataa gaaacaacaa ttagaaaatc gaatgaagta
      781 tggaaagctc tacattgatt caaatgcagt atgtacgaag gaagacggtg agctagttac
      841 gccaagtgta gtgaaatgga atactaggag gatatcgaat gcactctccc tctcttttaa
      901 cttccattct ctcagacata ctcatgctac acttcttctc gaaaatggcg caaaaatgaa
      961 agaaatctct gaacgattgg gccacagcag aatttcaatt acgatggata cttactcgca
     1021 tgtaacagat aagatgagaa atgaaacggt cgatatcatg gaaaatctta gaaagaattc
     1081 ttgatttttg ccaccgaaaa aaaccaccgg tggcaaaccg gtggcaaatc catgtaaaca
     1141 tggtttattt tttacactgt tttcaaacaa acccttgcta tttcttcggt tcgtcctcgt
     1201 ccattttcga taccgacagc tagcttataa cccaaggtat atcaacataa aaaaaaaaaa
     1261 aaaataggct gtgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctaatttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacct
//
LOCUS       DACEMO010000273         1898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.281, whole genome shotgun sequence.
ACCESSION   DACEMO010000273 DACEMO010000000
VERSION     DACEMO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.281"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..170
                     /locus_tag="IW728_002653"
     CDS             <1..170
                     /locus_tag="IW728_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1025976.1"
                     /translation="KYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGE
                     LEQMLSQPMEN"
     gene            665..1264
                     /locus_tag="IW728_002654"
     CDS             665..1264
                     /locus_tag="IW728_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1025977.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1328..>1898
                     /locus_tag="IW728_002655"
     CDS             1328..>1898
                     /locus_tag="IW728_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025978.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          682 a          299 c          365 g          552 t
ORIGIN      
        1 agaaatacag ccaccgcaag gaacagttcc aaaatgaaga gtcgatggaa cgtttcttag
       61 tctcgtcttt tgatacttac aaccaaaaat tcctaggtcg cagtcataaa ggtttccaac
      121 aggccgaagg cgagctcgaa caaatgctga gtcaaccgat ggagaattag aaacaatcta
      181 cagtagggaa gaaccattga cacaaaatta ttgacgctcc caactaaaag tgtcaaaata
      241 atgatgaaat catttagttc ttataaatta gaaaagagaa aataaatatt ccatatctac
      301 tttattggat ccgtaacatg actaaattct gaatactttt taaagcttat catttggtaa
      361 aactgaatat aagttaatta tatagactga aaattttgaa ttttcagaat agaataactt
      421 aaaagtactg acaaaatttt ttaaaggggc aatattaatg ttctgagata atcaaatcct
      481 accgaaaata tcaaaaatcc aatgtgacac aatcagattg tgtctcattc gagggaatca
      541 cacaatccca cgtggctcta ggcagaattg aatttttgct tggggctttt tccttatatt
      601 gattttgaat gagacttatt atatgttata atgcaattaa gtatcattgg agagggcgaa
      661 gaaaatgaag acggtagaac ctattcgaga caaaaaaaag atcgatgcga tgaaggcaat
      721 cttggcttcg ggaaaatatg gccaacgaaa tttggttctt ttttcgattg ggatcaacac
      781 cgcctatcgc atttctgatt tgcgtcaatt gaagctggcc gacgtgctgg aaatttcacg
      841 cggtcgcgtg gtggtcaaag atcgattagc gatgaaggaa caaaaaacag ctaaacataa
      901 ttcggtctat atttccaaca aattgcgaaa aacgattttg gagtacgttc aacaggaatt
      961 tcctgatcaa ctccttcagc aagattttga cggttatttg ttccctagtc gaaaagggac
     1021 gaatgaaccg ctcaaccgcc aaagtctgtg gcgaatcatt catgatgctg gcacggcaat
     1081 cggattgaaa gacattggcc cacattccat gcgaaaaacg ttcggttact ttctctataa
     1141 acaggggacc aaaacagaaa tcattcagtc cttgttgaac cattcttctc agcgcgaaac
     1201 attgcggtat attggtatta cacaagaaga caaagataca gctgtaaaaa gcttagattt
     1261 ataatataaa agttaagttt aaacatggat agttttaaat tagtagattg agtgttggga
     1321 ggaaagaatg agtacattat atattatagg aaacggtttt gatttaagtc atggtttaaa
     1381 aactagatac actgattttt caaaatgggt tgaaaaaagt catagtgaaa tattcgataa
     1441 aatagacaca atgataaatg aaaataatga ggtgagaagt acagctgatg ataagatagg
     1501 ttggtttaat tttgaaagta gtttgcatga gttaattcaa agtccagtct ttgaagatta
     1561 tgtagaagag tctaaggatt atctcatagg ttatggacat gaagattggt cagattctgc
     1621 ccatcatgat tttcaaaata tggtttatga ttatgttgtt tctctagaag aattacaaca
     1681 acttttcaag gaatggatac gcaaagaaat acagccgaca atagaacata aaattccaaa
     1741 ataccaattt tcgtccgatg tactattctt tacattcaat tatacagatg ttttggaaat
     1801 tctatacaag caaccgaaag aaaacatata tcatattcat ggcagttatg acgatataat
     1861 aatcggccac tccgagaatt ttgacaactt tataggag
//
LOCUS       DACEMO010000274         1893 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.282, whole genome shotgun sequence.
ACCESSION   DACEMO010000274 DACEMO010000000
VERSION     DACEMO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.282"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW728_002656"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW728_002656"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1025979.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IW728_002657"
     CDS             complement(1431..1823)
                     /locus_tag="IW728_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1025980.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          530 a          434 c          327 g          602 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tat
//
LOCUS       DACEMO010000275         1874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.283, whole genome shotgun sequence.
ACCESSION   DACEMO010000275 DACEMO010000000
VERSION     DACEMO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.283"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1541
                     /locus_tag="IW728_002658"
     CDS             <1..1541
                     /locus_tag="IW728_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1025981.1"
                     /translation="AITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGF
                     WIIGEERLVRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQ
                     VIIRMRHLLEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDL
                     SYRVMQQVFDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQ
                     DPQLLTNLYAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNIL
                     INDSFVAYIALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAF
                     ASVLNAQEMIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSN
                     QQERHNLNLRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVF
                     LMVHRITFDQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKE
                     GASS"
     gene            1541..1873
                     /locus_tag="IW728_002659"
     CDS             1541..1873
                     /locus_tag="IW728_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1025982.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          666 a          290 c          361 g          557 t
ORIGIN      
        1 aagctattac tggaatgaaa atggccaacg cattagatgt cagcaaagat acgatcatga
       61 atgatttaga tgtgctggaa aataattttc ttaaggaagg attgaccttg aatcgtcagg
      121 cgcgaaaggg tttttggatc ataggagaag aacgtctagt tcgattaacg attgaagaga
      181 ttcttcaaaa agagttcacg gattatgaca tttataaatt aatgtcattg ttattgaatg
      241 gcggtgagac tgaatacttt gaaatgtatt ctgctactgc tacacctatc caagaggtat
      301 ttaatcaagt aatcattcga atgcgacact tactggaatt tgaaaatcta gagaaactga
      361 attacgcgga attactaaat attctgattc gagtgacaat tgctaccgtt cgcttacgca
      421 aagaggctac gattggtcgt tatcaattgg ttgctgaaca agaattagac aaaaacgatc
      481 tttcttatcg cgtcatgcag caggtctttg attattatca gttgccactg ttcgaggatg
      541 aatatcatta tatctatagt gatacattcg acacgattca ccagcaagac gtcatgaaac
      601 tagttgagaa tgtgatttat aaagtaagta aagcaatcaa ccattctttt tatcaagatc
      661 cacaattgtt gacgaatctg tatgctcatt tatccatgcg attatcgaga aagaaaaaat
      721 ttgtcaatga atataatcct tttaaagaag atatcaagca gaaatacccg gaattatttg
      781 atgcgatcaa gcaagcagtt cgacaagaaa taagtgaaca aaatattctg atcaatgatt
      841 ctttcgtggc atatattgcc ttacactttt tggtttccta cgaaagagaa gaagatctac
      901 gaagcgttag agccgtctac atttgttcaa cagggttggg tgtgactagt ctgatcaagc
      961 aaaagatcaa tgaagaatta aataatatca ctatcgtggc atttgcttca gtattgaatg
     1021 cgcaagaaat gattgctacc aaagatcctg atttggtgat tagtattttt ccaattaatg
     1081 gcatcgatcg tcctttcatc aaagtccatc cattacccac tgaacaggac ttgaaaaaaa
     1141 ttcaacaaat aataaaaaaa atacttgctt cttccaatca gcaggaaagg cataacttga
     1201 atcttagaga aacgtccatc aataaagata ctttagaaga ctttagcaag gaattgattt
     1261 tgaaagctta tacgatttat gaacgtttac ttgtcatttt ggaagcagaa ttgatccaag
     1321 aatatcgaga agcttttttg cttcatgtat tcttaatggt tcatcgtatc acatttgatc
     1381 aacaatatat ggcagaaggt cgtcaagtag accagattat tttagacaaa ggaacactgg
     1441 taggaaaaat cgaagcagtt tttgcagaaa ataatttagg aatcaatcag tcagaaatca
     1501 gtgcattatt ctcatatata aaagaaggag cgtcaagtta gtgaaaatgt cagacgaggc
     1561 tttaaaaatt atcgaggaaa gtcaaaatcg ggaaaagtta aaacaagtga ttgatctaat
     1621 tgaagagcga ttagtagaac atgctattgt accaaccgag ttgcagtgga ccattctaat
     1681 taatcacttg aatgagatgc tgaaacgttc tcaaagaaag gaaacgattc ccgtagtcga
     1741 tcgggatttg ttcaaagaag tatccaaaga agccataact atttctgatg aaatagttag
     1801 aagtatcggt aatttaaccg atgatgaaat atatgttttg tcgattcatt ttgaaacagc
     1861 aaaaaataat taat
//
LOCUS       DACEMO010000276         1871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.284, whole genome shotgun sequence.
ACCESSION   DACEMO010000276 DACEMO010000000
VERSION     DACEMO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.284"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            277..873
                     /locus_tag="IW728_002660"
     CDS             277..873
                     /locus_tag="IW728_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1025983.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            886..1785
                     /locus_tag="IW728_002661"
     CDS             886..1785
                     /locus_tag="IW728_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1025984.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1788..>1871
                     /locus_tag="IW728_002662"
     CDS             1788..>1871
                     /locus_tag="IW728_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1025985.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVAT"
BASE COUNT          708 a          292 c          383 g          488 t
ORIGIN      
        1 caagaaaaga acacgccgca ctgcaaaata atggagggtt aatcttatgc gtaataccac
       61 acacaaacaa caagaaatcg aaaatcaaaa agccaaaatt cgttcagaac aggaaaaaat
      121 gcgtgccagc aaacgaagac tgcagcaatt gaagaaccag cagaggattc aaaaagaaat
      181 cgaattagat cgataaaatc ttcctaacta ttgtatacgt ataaacttat gttatactgt
      241 ttttataagt ttatacgtat acaaaggagg gagaaaatga caaaagcaat ggtatacaag
      301 ctgctagaag aatttaatgg aactttggct ttgaaggatg ccaaaaaagc gggcatttct
      361 cctgtaacga tcaaacgtat ggtagatcga ggggagctgg atcgagagta tccaggtttt
      421 ttcaccttac ctggtcaatt tcctgatgaa ttgtttatgg cgcaaaaaaa atatgaacgc
      481 ggcattattt cacatattac cgcgttagat ttgtatgatt taacagatat gatccctaga
      541 cagatcgatc tgactgtacc ttacggttat cacgtttctg aaaaaggatt gaaggaattc
      601 gcagttgagc tacactatac aaaagcggaa tggtatgaat taggtaagat tgaaataaaa
      661 agtcgctatg ggaatcctat tatagcttat gatccagaaa aaaccttgtg tgacatttgg
      721 aatccctggt ataacgttga ggatgaaatc aaagtaaaag caatcaaaaa ctacatggag
      781 tcggacagaa aaaatcttag aaaattgaat gagtatcgac gaattttgcc tacagataaa
      841 acgatgcgct cttatattat ggctttaaat taaaggagaa tacacatgac acctacgcaa
      901 ttaaaagcaa aagtgaagaa tatttctaga gaaaaagaag ttgatccgca gcttgtttta
      961 agacatttta tgatggaaaa attcttggaa aaaatctcag agtctcctta tcgtgaaaat
     1021 tttgtgttaa aaggcggctt cttaattggc tcaaagtatg gcattgaaaa tcgaacaact
     1081 aaagacattg atacgacttt acgggaaatg aaagtaacca aagaaacatt gacaacggtt
     1141 ctaaacgata ttttttcaac gccgacgaaa gaaggcattc aatttgagat tcaaggaatg
     1201 aaagaaacga gggaagctga ttattatcca gggtttagct tacgtgtact ggcacatttg
     1261 gaaaacatgc ggccagattt taaggtagat gttactacag gtgactcgat ctatcctgcg
     1321 acgatcactc acagtcataa attgatgttt gaggatcgaa cgattccttt ggaatcctat
     1381 cctacagaac aaattattgc tgaaaaatta agtgcaacct ttgatttttt aacagataat
     1441 tcgagaggaa aagattttta tgatctctat actattccta aaatggagaa gatcgatgag
     1501 aagaccttga aagatagtgt gagaaataca tttacacgtc gaggaaaaac tgatccgtta
     1561 aagatatact atgaaaagga tatggatgta ctgaaaaata gtcacgagct aaaagatacc
     1621 tggaagaaat atcaaaaaag caatccttat gctgcgtcga tcacgtacga tgagacaatg
     1681 gattcgatta gtaatttgat ggaaaaagtg attcacgtcg aagaaaaaga gcgaaaacta
     1741 gcttatcaaa gacaccaaca gatgaataga gaaatggaga gataaagatg attaataatg
     1801 tgacattagt aggacgctta accaaagatc ctgatttacg ttataccgca agtggtactg
     1861 cagtagcgac t
//
LOCUS       DACEMO010000277         1817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.285, whole genome shotgun sequence.
ACCESSION   DACEMO010000277 DACEMO010000000
VERSION     DACEMO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.285"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            255..>1817
                     /locus_tag="IW728_002663"
     CDS             255..>1817
                     /locus_tag="IW728_002663"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1025986.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKL"
BASE COUNT          602 a          314 c          400 g          501 t
ORIGIN      
        1 tttttttgca ctcataaagg acttaagaaa taatttctca aaggatatcg aaaaagaaaa
       61 aattgtaagc ggatagtatt catttgagta cttgactttc ccttagtcag cttgtattat
      121 acaccttggg caccctttta gagggtcaga agtttacaga aatttcggct ccctattctt
      181 attgaagaat agggagcttt gtttattgtc tgagactttt cctgcacata aattaaaaag
      241 gagtttttga acttatgaca aaatatattt ttgtgaccgg cggtgtcgtt tcatcaatcg
      301 gaaaagggat tgttgcagct tctttaggac gcttattgaa aaaccgcggc ttaaaagtaa
      361 ctatccaaaa atttgatcca tacatcaacg tggatccggg gacgatgagc ccatatcagc
      421 acggagaagt attcgtaaca gatgatggtg ctgaaacaga tttggatctg ggccactacg
      481 aacgttttat tgacatcaat ttgaacaaat actccaacgt gacgacagga aaaatctatt
      541 ctgaagtttt gcgtaaagaa cgtaagggcg aatacttggg agcaactgtt caagttattc
      601 ctcatattac aaatgagatc aaagaaaaaa tcatgcgagc agctaaaatg actgattcag
      661 atgtgatcat tacagaagta gggggaacag taggggatat agaatctttg ccattccttg
      721 aagcacttcg tcaaatgaaa gcagatgtcg gtacagataa tgttatgtat atccatacaa
      781 cattgattcc ttacttgaaa gctgctggag aaatgaaaac taaaccaacg cagcacagtg
      841 taaaagagct acgtggttta ggtatccaac cgaatatttt ggtggtacgt acagagcaac
      901 ctgtttctca aagcgtgaaa aacaaattgg cacaattttg tgatgtcgaa ccagaagcag
      961 ttatcgagtc gccagatgta gatacacttt actccatccc attagtttta caagcacaag
     1021 gaatggatca aattgtttgc gaccatttaa aactagatac accagaagca gacatgacag
     1081 aatggaaaga attagaagaa cgtgtcttga acttgaagaa aaaagtacgg attgcgcttg
     1141 tcggtaaata tgtcgagtta ccagatgcat atatctcagt agttgaagca ttgaaacatt
     1201 ctggtttcgc ctataattca gacatcgaga tcgattggat caaagcacaa gaattaacga
     1261 gagaaaatgt agaagaacgt ttgaaagacg cagatggaat cttggttcct ggtggatttg
     1321 gtgatcgcgg agtagaaggg aaaatcgaag cgattcgtta tgctcgcgaa aatgacgttc
     1381 cgtttttagg tatttgtctt ggtatgcaga tggcttgtgt cgaatttgca cgtaacgtcg
     1441 ttggattaga agatgcgtca tcagctgaaa cgaatccaga tacagcaaac aatattattg
     1501 atttaatggc tgatcaggaa aacatcgaga atttgggtgg aactttacgc cttggattat
     1561 acccatgtaa aatcaaaaaa ggaacgaaga cggctgaagc atatgatgga gccgatgttg
     1621 tgcaagaacg tcaccgtcac cgctatgagt tcaataacaa atatcgtcaa ttgtttgaag
     1681 aacaagggct agtcttttca ggtgtctcac cagataaccg tttagtagaa atcgtagagc
     1741 tgccagagaa aaaattcttt gtgggatgcc aattccaccc agaattgatt tctcgtccaa
     1801 atcgcccgca aaaatta
//
LOCUS       DACEMO010000278         1804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.286, whole genome shotgun sequence.
ACCESSION   DACEMO010000278 DACEMO010000000
VERSION     DACEMO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.286"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            75..503
                     /locus_tag="IW728_002664"
     CDS             75..503
                     /locus_tag="IW728_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1025987.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="IW728_002665"
     CDS             490..732
                     /locus_tag="IW728_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1025988.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="IW728_002666"
     CDS             complement(1032..1247)
                     /locus_tag="IW728_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1025989.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="IW728_002667"
     CDS             1354..1668
                     /locus_tag="IW728_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1025990.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
BASE COUNT          577 a          271 c          364 g          592 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatc
//
LOCUS       DACEMO010000279         1801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.287, whole genome shotgun sequence.
ACCESSION   DACEMO010000279 DACEMO010000000
VERSION     DACEMO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.287"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..605
                     /locus_tag="IW728_002668"
     CDS             <1..605
                     /locus_tag="IW728_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1025991.1"
                     /translation="PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVD
                     KKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEA
                     GTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQS
                     SKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            739..>1801
                     /locus_tag="IW728_002669"
     CDS             739..>1801
                     /locus_tag="IW728_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1025992.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQVIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESI"
BASE COUNT          703 a          226 c          340 g          532 t
ORIGIN      
        1 taccaaaagg aactacttat acttggaaag aaaaagtaga tgttagtaca gctggaaaca
       61 aaaaaggtac tgtagtagta acatatccag atggatctaa agaagaagta gaagttacta
      121 tttctgtagt agataaaaaa gcaccgaata aacctcaagt tgatcctatt acagagggtg
      181 accaaattgt tactggtaaa actgaaccaa atgcagaggt gacagttaca ttaccagacg
      241 gaagtcaaca ccatgggaca gctgataaaa atggtaattt tacagttaaa gttcctaaat
      301 tagaggcagg gacaaaagtg atagtaactg caactgatga atctggtaat actagtgaac
      361 ctactaatgt agttgtttct tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta
      421 gtaagactga caatcaaggt agcaacagca atcaagacaa aaatagaggc aaaagccagt
      481 cctcaaaagt ttttcctaag acgggagaga gtgacagtaa tatctttact attagcggtg
      541 gtttaatact gttaggaacg ttaggattat tagggtataa aaatcgaaaa aaagagaatg
      601 aatagaaaat caatttctta agtcttctct gagaagagga agaatctttt tcttcctctt
      661 tttgcttttt tatgatggga aataagtgtt agttaaagat gaatatactt tttatagaaa
      721 ggaaaagaat gtaattaagt ggaaaaaata gtaattattg gttcaaacca ttcagggcta
      781 gccgcagctc gatttctaaa cttaagcgat tcaaattatg aagttattct aatcgataaa
      841 aaatcagaaa ttagttactt ggaatgtggg acatcgttat ttataaaagg agaagtaagt
      901 aatttagaag atctttttta tcaaaataga atgacactaa attctgagat aaaaaagtta
      961 tttctagaga cagaagttga caaaattgat attcaaaata aaaagattag ttgtgtaaaa
     1021 aaagatggta caagattctc tcaagaatat gacaaagtta tattagctac aggttcaaag
     1081 caaattatgc tagatatacc ggggaataat ttagctaata ttttttcgat aaagaatttt
     1141 ccaaatgcaa aagagatttt taaagctttg gatgatagta ctattaaaaa agtaggaatt
     1201 attggagcag gatatattgg agttgaaatt gctgaagcca taagaaaaag agggaaagaa
     1261 gtatatcttt tcgatgttgc agatagagtg ttgtcgactt attacgaccg tagtttttca
     1321 gataaagttg aagaaatact atcaaaaaat ggaatacatc tttgtctatc agaaagtcct
     1381 aaaaaatatt taggaagaaa aaagattgaa aaaatagtca ctacaagtgg tacccaatac
     1441 tctatggata tggtcgtaca agttatagga tttctatcta atagtccaat tggggaaaag
     1501 gaactcttga gagatgttag tggagcatat ctaactaatg aataccagca aacaaatata
     1561 aaagatatat atgctattgg ggattgtgct acaacttttt ttacgtccat taatagaagt
     1621 agtgttgatt tttctatttc taatgctcta agaacagctt atattgcaac tcaacatatt
     1681 aacaatcaag attttacacc ttctggttcc caatttacaa atggcttttc tatatttaat
     1741 tacaattttt acgcagcagg tttgaattta aaaacagcac gattattaag tgagtctata
     1801 g
//
LOCUS       DACEMO010000280         1794 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.288, whole genome shotgun sequence.
ACCESSION   DACEMO010000280 DACEMO010000000
VERSION     DACEMO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.288"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..534
                     /locus_tag="IW728_002670"
     CDS             <1..534
                     /locus_tag="IW728_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAQ1025993.1"
                     /translation="ENAKKEAEKNELPNVSFIKQSLEEFIQSNETYEMVFTAANPALV
                     HLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDPNTSPVVMDQFETYLI
                     DEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRKLFSEKREILSTTKLT
                     YRLLLWSADKKRTFHDS"
     gene            complement(556..1710)
                     /locus_tag="IW728_002671"
     CDS             complement(556..1710)
                     /locus_tag="IW728_002671"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1025994.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT          572 a          346 c          329 g          547 t
ORIGIN      
        1 gaaaatgcaa aaaaagaagc agaaaaaaat gagttgccaa atgtttcctt tatcaaacag
       61 tcgttagaag aatttatcca atcaaatgag acatatgaaa tggtttttac agcagcaaat
      121 ccagcattgg tacatcttat tcaattagaa aaattgatga aaaagtcaag caaatggtgc
      181 gtgataatca gagtagtgga atcaatcgat gatgtttttt atcctatcga aaagcaacta
      241 ggaatgatag aagaagatcc taatacatcg ccagttgtaa tggatcaatt tgaaacatac
      301 ttaattgatg aaggattcaa agtgaagaaa aaagatttta cttattttac acaggaatca
      361 attactgaag aattgatgaa gctatactat gacgcgtacg caaaagatcc tgttgtgacc
      421 aggtacattc gcaaactatt cagtgaaaaa cgagaaatcc ttagtacgac taagctgacc
      481 tatagactgc tcctttggtc cgcagataaa aaaagaacct ttcacgactc gtgaagggtt
      541 ctttttatga ttttattata ttttgtagta acgaatatca ttttcgatct ttgtaatcga
      601 aaaaggtaca tctgaagtat attctgcaca ttctccgtgt tcaggaagcg tctcactgaa
      661 aatcgtttca tattcgtcaa cagtgatccg tgttcgagaa tctagcatct cttgatgaga
      721 ttgtgcgaag agatattctt gatagttttc ttccagataa cctgtaaaaa attcacttac
      781 tgcaccagaa ccgtagctga atagaccgat gcgatctcct ggctgtagtg atttcgaatt
      841 ttccagtaga gaagttagcc ccaggtataa tgaaccagtg taaagattac cgattcgtcg
      901 gctgtaacgg atgctttctt catagcgagc cataagacgt tcctgatttt cttcgtctgt
      961 ttggtctaat acgctttgca atgccttttt tcccatctta gtatacggaa tatggaaagc
     1021 aatcgcctga taatcttcga gtcctcgacc cgacaattct ttatgtcgat tccaaacttt
     1081 ttggaatgat tcgatatacg tagaattaga taaaggacca tcaacaacag gaaattcgct
     1141 ataatctgga cgccagaaat catagatatc ttctgtcaga aatacgctgt cgtcttcaat
     1201 cgataaaata cgcgggtttt gagtgatcat catcgcaaca gcaccgacac cttgcgtcac
     1261 ttcaccaccg cttgccaagc cgtaacgagc aatatcactt gctatgacta gtacttttcg
     1321 ttctggatga tttttgacat attctttcgc catgtgcaaa gctgcggtgc caccgtagca
     1381 agcctctttt acttcaaaag aacgagcaaa agattggatt ttcagtaaat ggtggataat
     1441 taccgcactc gcttttgaat gatcaatccc ggattctgtt ccgacgatga ccatgtcgat
     1501 tagctcacgg tctttttctg ttacgatttt gctggcagca tttgctccta gtgtaatgat
     1561 gtcttcattt gcacgattga ctgccatttg gtcttgacca atccctatat ggtattttgc
     1621 aggatcatcc ccacggcttt ctgccaactc tgtcatatct aaatataaat taggaataaa
     1681 aaaggaaaga cgatcaatcc ctattttcat ttcaacacct ctttacttat gataaaaaac
     1741 ttactttact aaggtatcat aaaaagatta aaagaaatat aaaaaagaga atat
//
LOCUS       DACEMO010000281         1794 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.289, whole genome shotgun sequence.
ACCESSION   DACEMO010000281 DACEMO010000000
VERSION     DACEMO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.289"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..70
                     /locus_tag="IW728_002672"
     CDS             <1..70
                     /locus_tag="IW728_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1025995.1"
                     /translation="ITRQGATNLKHRLYKKIQKMLV"
     gene            164..355
                     /locus_tag="IW728_002673"
     CDS             164..355
                     /locus_tag="IW728_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1025996.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            869..1201
                     /locus_tag="IW728_002674"
     CDS             869..1201
                     /locus_tag="IW728_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1025997.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1497..1652
                     /locus_tag="IW728_002675"
     CDS             1497..1652
                     /locus_tag="IW728_002675"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1025998.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          653 a          297 c          310 g          534 t
ORIGIN      
        1 gattacaaga cagggtgcga caaatttaaa acatcgactt tacaaaaaaa ttcaaaaaat
       61 gctggtttaa tatctgcata ataactaatt ttgtatgctt ctttgctcgt tctcctcctt
      121 cccaaagaaa tagattataa gtccatggga agaaggtaga tttatgaatt ctttaaaaga
      181 agatttcatt ttagcaaagg ctggaaatga agaagctgtt gaggcaattc tcaaaagatt
      241 tagctctttg attcataagc aatcctggag aactggaaaa tatgatcaag attgttatca
      301 agaatgtatg cttgcaatat atctagctat ctcgaaattc gaaattaaag aataactcaa
      361 ctaagggagt ctgtgacata agtaaaaaga tagtttcaaa tttcgaacaa taaatttgaa
      421 actatcttct ttataattat aaagtatcga ttttacagcg ttctataatg attcatagaa
      481 tctaaaacga atgattttaa ttatcaacgt tttttaattc gtttttagct taaaaataac
      541 ttttgtcaca gtctcactcc taaaattact ttatttacgt atgaagtggg caggaggtcg
      601 aatccagaac tgggctatgg tacttcaacg aggacttctc ctctagaatt gaaaagcatg
      661 cgatttacct tccgtaacga cggtagattt attcaactat gtgtccaatc aaaatttgtc
      721 tgatttcaaa aatagtcaag agaattttgg ttgttaaaaa ctcttatcta tcaacttcca
      781 cagtttagtt gacaaaccct gatactaagt tgggctatga tacacttttc ttgactatca
      841 gttacgttac cggaaggaat gaactctatt gagaaaaaca tatgatcgta aatttaaact
      901 aaagatttca caggatatcc ttgagaaaaa agtaactacc aagaaaattg ctgaagaata
      961 caatatctct cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat acgggaaaaa
     1021 tgcttttgct ggacaaggaa aaagattacc tgataaagca gatttctaca ttcttgaaca
     1081 ggaaaataaa cgactaaaag aggaagacga gtgttctacg aacacaaaga tcgatacgga
     1141 tctccaggga tcactcaaaa actttacgat gaaggaatcg aaatcaataa acgtgttgta
     1201 gctgttctta tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg caaatcttct
     1261 catggtagag aaaaaacaat caaagaaatg gtaaaaaaat gttgaatcga taatttgaac
     1321 agaaccaaat tgatgctgtt tgggtgatgg atattactta cattccatgt aatgacggtc
     1381 gcctctattt gtccacctat attgacttag caactcgtat tctacgttac tatatggtag
     1441 attcacacat gaaaaaagag attgtcgtcc gtccattaca aatttattaa ggagaattgc
     1501 ctacggtaat tcattccgat agaggtagcc agcatcggtc ttttatttat caagagctgt
     1561 tgaacgacca ccacatcaca cattcagtga gccaaactgg gactcctgtt gacaatgcag
     1621 taattgaagc gtttcatcga tcaatcaaat gagaattaat cgactccaac aaatataaaa
     1681 gtaaaataga gatgaaggtg ttaatccaag attatttaga agactactac ccaaacaaaa
     1741 gaatccatac aaaatttatg atgactcctt gacaatttga agcgaagaaa ataa
//
LOCUS       DACEMO010000282         1785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.290, whole genome shotgun sequence.
ACCESSION   DACEMO010000282 DACEMO010000000
VERSION     DACEMO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.290"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            401..1657
                     /locus_tag="IW728_002676"
     CDS             401..1657
                     /locus_tag="IW728_002676"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1025999.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
BASE COUNT          615 a          314 c          374 g          482 t
ORIGIN      
        1 aaaaaatgag gaatttaaaa aaaatatcct ttttctgttg acaaagaaaa agttgcgctc
       61 tatgatagac acatattaat aataacaaga cgaagatcga aacgaagtag ttgttaaagc
      121 caatgtttca gaaagctggt ggtcgctgca aaccagtaca tgctttaaag cgaattacat
      181 ttcaggagtc tttggcagtg attgccacgc gacagcgtta aatgttcgag aggtatgttg
      241 gatatctaga catacaaatt gggtggtacc gcgagatttc gtcccttggc ttatatgcca
      301 agggactttt tttatttcct atcctatcct gaagaaataa ttaattacat atgctagcag
      361 gttcctgctt tcataaaaaa actacacgga ggtcagtaaa atgaatatta ttgatgaatt
      421 aacttggcgc gacgccatta atcagcaaac aaacgaagaa agacttcgcg agctcgttga
      481 agaaaagagc atttcacttt attgcggcgt tgatccaact ggtgattcga tgcatatcgg
      541 acatttgatt ccatttatga tgatgaaaag attccaattg gcaggccatc atccatatat
      601 tctgattggc ggcggaacag gaacgatcgg cgacccaagc ggccgcaaaa cagaacgtgt
      661 gttgcaaacg atggaacaag tgcaacataa cgtggacgca ctttccaatc aaatgagaaa
      721 attgtttggt aaagatgcaa atatcacatt tgtgaacaac tacgactggt tgtcaaaaat
      781 ctctttactt gaatttttga gagactacgg taaaaacttc aacatcaaca cgatgttagc
      841 aaaagatatc gttgccagcc gtttggaagt aggaatctca tttacagagt ttacttacca
      901 aatcctgcaa tctatcgact ttttgcatct gcataaaaca tacgatgtgc aattgcaaat
      961 cggtggagca gaccaatggg gaaatatcac agctggatta gacctaatcc gaaaattaga
     1021 aggaccagaa gcagaagcgt tcggtttaac gatcccattg atgctgaagg ctgacggaac
     1081 gaaattcgga aaaactgcag gcggtgccgt ttggcttgat ccgaagaaaa cttcaccatt
     1141 tgagttctac caattctggt tgaatcaaga tgatcgcgat gtagtgaaat acttgaaatt
     1201 ctttactttc ttgtctcaag aagaaatcga agacttagcg aaaaaagtag agacagaacc
     1261 tgagaaacgt gaagcacaac gccgtttggc agaagaagtt acaagatttg tgcatagtga
     1321 agaagacttg aaagaagcgc aaaaaataac gcaagcattg ttctctggaa atattaaaga
     1381 actaaatgcg gaagaaatcg cgcaaggatt cggtaaaatg ccgaacgttg aaatttcaag
     1441 cacacctgaa aatatcgtgg aactgcttgt ttccacaaaa attgaaccat ctaaacgtca
     1501 agcacgtgaa gatgtttcaa acggagctat aagtattaac ggtgaccgcg ttaccgattt
     1561 aaattttgtc ataaatccat ccgatgaatt cgatggtaag tttgtggtta ttcgaaaagg
     1621 taagaaaaat tactttttgg ccaaagtaat tgattagaat ttagtaagtt tttaccttga
     1681 aaaagggaaa tactattaca acattccaat tttgaaaccc atcctaaaat agaacgctgg
     1741 ttgtcgttaa tacaatcttt gcgagggagt ttacatttgt aaatt
//
LOCUS       DACEMO010000283         1765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.291, whole genome shotgun sequence.
ACCESSION   DACEMO010000283 DACEMO010000000
VERSION     DACEMO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.291"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            150..>1765
                     /gene="tkt"
                     /locus_tag="IW728_002677"
     CDS             150..>1765
                     /gene="tkt"
                     /locus_tag="IW728_002677"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1026000.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVK"
BASE COUNT          587 a          337 c          403 g          438 t
ORIGIN      
        1 gaaatccaaa agaaaaaagg cttacacaac cgaaatgaca acttttagta aaaaatactg
       61 aaaaagattg ctttttcgtg tgacatctta tagaatgaaa gtgtaagaaa taaaggtaaa
      121 aaaaacctaa attaatagga ggggttattt tgtttgacaa aattgaccaa cttggcgtta
      181 acacaattcg tacattaagt attgaagcag tccaaaaagc aaactcagga catcctggat
      241 tgccgatggg cgcagcacct atggcatatg cgttatggac aaaacacctg aaagtaaatc
      301 caacgacatc aaaaaactgg gctgaccgcg accgtttcgt tctttcggca ggacatgggt
      361 cagcaatgtt gtatagcttg cttcatttag ctggctatca agtgacaatc gatgatttga
      421 aacaattccg tcaatgggat tctaaaacac caggacatcc tgaagtacat catacagatg
      481 gtgtagaagc cactacaggg ccattaggac aagggattgc aatggctgtc ggtatggcaa
      541 tggcagaagc gcaccttgca gcaacgtata acaaagaaaa ctttaatgtg atggatcact
      601 atacgtatgc aatatgcggt gacggagact tgatggaagg ggtatctcaa gaagcaagtt
      661 cgatggcagg tcatatgaaa ctagggaaat tgatcgtttt atacgattca aacgatattt
      721 cactagacgg tccaacttca aaagctttca cagaaaacgt tggtgctcgc tatgaagcat
      781 atggctggca acatatctta gtgaaagacg gaaatgacct agaagcaatc tctaaagcga
      841 ttgaagaagc aaaagctgaa acagacaaac caacattgat cgaagtaaaa acagtcatcg
      901 gctatggtgc tcctaaagaa ggaacatctg ctgttcatgg tgctcctctt ggtgaagatg
      961 gcatcaagat ggctaaagaa gtttatggtt gggagtatcc agatttcact gttccagaag
     1021 aagtagctgc ccgcttccat caaacgatga tagaagaagg acaaaaagct gaagatgcat
     1081 ggaatgaaat gtttgccaat tataaaaaag cttatcctga attagcacaa caatttgaag
     1141 atgcttttga tggcaaacta ccagaaaact gggatgcaga attgccaact tatgaagttg
     1201 gaagcagtca agcaagccgt gtttcaagta aagaagtcat ccaagaatta tcgaaagcta
     1261 taccaagctt ctggggtggt tcagcggact tgtcaggttc caataataca atggtggctg
     1321 cagataaaga tttcactcct gaacattatg aaggacgcaa catctggttc ggtgtccgcg
     1381 aatttgcaat ggcttcggct atgaatggta tccaacttca tggcggaact cgtatctacg
     1441 gtggaacatt ctttgtcttt gtcgattatc taagaccagc agttcgttta gcagcaatcc
     1501 aacatactcc tgttgtttac gtcttaacac atgactcggt tgctgtcgga gaagacggac
     1561 caacgcacga accgattgaa caactagcat ctgttcgctg catgcctggt gttcaagtga
     1621 ttcgtccggc agacggaaat gaaacacgtg ctgcttggaa agtagcgatg gagacaacag
     1681 atgcgcctac tatccttgta ttgagtcgtc aaaaccttcc agtattgcct tctacaaaag
     1741 aagtagcaga tgaaatggtg aaaaa
//
LOCUS       DACEMO010000284         1744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.292, whole genome shotgun sequence.
ACCESSION   DACEMO010000284 DACEMO010000000
VERSION     DACEMO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.292"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(272..1564)
                     /locus_tag="IW728_002678"
     CDS             complement(272..1564)
                     /locus_tag="IW728_002678"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1026001.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          497 a          373 c          306 g          568 t
ORIGIN      
        1 caatcagata aaaatagcac tccatatttt tcatctgatt gggcttagtg ccgtagatgc
       61 aagcttttga acaccgtttg ttcggaaaaa gggactgtga cacggttttt gtcacagtcc
      121 cttattctga ataaacagtg ttcaagagac agcctatcgg caataaggct aaacgtctct
      181 ttcactacct cttcaagtta ttattatcgt atagataaac acttcgactc gctctggtaa
      241 gaacgtcctg attcagtcgt cgtttttatc cttagatttg cgcccagacg ctttctaaga
      301 tgtttgtttg tgttctatca ggtccgacag aaaatgttga gatacgtaca ccaacaagct
      361 ctgagatacg acgtacatag ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc
      421 ctgtaatgtc ttctgaccag cctggcaatt cttcatagat tggtttgcag cggttcaact
      481 ctttcaagct tgctggatag tggtagatta gttcgccatc taattcataa gccgtacaaa
      541 tttttactgt ttccaatcca cttaatacat cgatcgagtt taaagatagg ttggtaatac
      601 ctgaaactcg tttagaatgg cgcataacta cagaatcaaa ccagcctaca cgacgcggac
      661 gaccagttgt cgtaccgtat tctcgtccta cttcgcggat ttgttctcct gtttcatcaa
      721 ataattcagt tggaaatggt ccatcaccga cacgtgacgt ataggctttg cagacgccga
      781 caactttatt gatttttgaa ggccctacac cgcttccgat tgttacccca ccggctactg
      841 ggttagaaga agtaacaaaa ggataagttc cctggtcgat atccagcatt acaccttgtg
      901 cgccttcaaa caacacgcgt ttgcctgcat ccaaagcatc attcaagata acggaagtat
      961 ctgtgacgta ttgtttgatt tgttgtccgt attcataata ttcttcaaaa atatcgtcaa
     1021 aatcaagtgg ttctgaatca aacattttta caaattgacg gtttttgtca tctagattca
     1081 aacgaagacg ttcttcaaaa atttctttat ctaataagtc tgcaatacgg atacctacac
     1141 gagctgcttt atccatatag gcaggtccga ttcctttaat cgttgtacca atcttatttt
     1201 cgcccttagc atcttcttgt aattggtcca atttgatatg ataaggcaag attacgtgcg
     1261 cgcggtcaga aatacgcaaa ttacttgttt ctacaccttt ttcatgtaaa taagctaatt
     1321 ctttaaccaa tgatttagga ttgactacaa cgccgtttcc gatcacactg atcttgtctt
     1381 tataaaaaat cccagaagga atcaaatgca gtttataagt cacaccgtta aacttaattg
     1441 tgtgccctgc attgtcgcca ccttggtacc gtgcaatcac ttcagcattt tcactgagaa
     1501 agtctgtgat ttttcctttc ccttcatcgc cccattgcgt acctactact actacagatg
     1561 acatgtgaac acctcgttct atattattta atcctgaacc attgtatcaa taatagatac
     1621 atatttcaaa taaaatcgaa tgatttttat ttttatttta taaaaacgct taaaaaacga
     1681 actttatctt tttagaatgg ttattaaaaa agaggttgta acagaagtgg tcagcttcaa
     1741 gaaa
//
LOCUS       DACEMO010000285         1741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.293, whole genome shotgun sequence.
ACCESSION   DACEMO010000285 DACEMO010000000
VERSION     DACEMO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.293"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            170..838
                     /locus_tag="IW728_002679"
     CDS             170..838
                     /locus_tag="IW728_002679"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1026002.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            902..1525
                     /locus_tag="IW728_002680"
     CDS             902..1525
                     /locus_tag="IW728_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1026003.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            1581..>1741
                     /locus_tag="IW728_002681"
     CDS             1581..>1741
                     /locus_tag="IW728_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1026004.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARL"
BASE COUNT          652 a          286 c          371 g          432 t
ORIGIN      
        1 atcagataaa aaacaaaaag caagtcaaaa tggatcgaaa aagttatact ttttttgagt
       61 gcccgaattg tcatcaaaag cagcgtgcac ctaaaggaaa aggacgaata cgtgtgacat
      121 gcaaaacatg tggtataaaa ttcgaaacga atgtttaagg gagaaaagaa tgaaattagt
      181 attattacgt catggcgaaa gcgaagcaaa ttttgaaaat tactggacag gatggctaga
      241 tgtcgcctta acagaaaaag gaaaagaaca agcagcagtt gccggtcaaa aaatgcttga
      301 agccggactt caattcgatg ccgtttatac ctctgttttg aaacgtgcga ttcttaccgg
      361 acaagtcgca ctagaagaaa tgggtcagtt atggcttcct ataataaaaa catggcgtct
      421 aaatgaacgg cattatgggg atcttgtggg taaaaacaaa gataagatga aagaaatata
      481 cggaaaagat caagtaaaaa catggcgcag aggatatacg gaagttccgc cgttaaccgc
      541 agaaaataga tttgaccgca gatatgatca cttggatccg catttgatcc catacggaga
      601 aagtctggaa atgacagtga atcggatcat tcccttatgg caagatcatc tagcaccaaa
      661 actaaaagat cggcaagatt tactagttgt gggtcatggt aatagcatcc gagcactgac
      721 aaaatattta gaagatgttc ctgaagatca aatggatacg atcgatatcc caaatgcaca
      781 accaatccag tatacattag cagatgattt gacaattctg gacaaaaaaa tactttaaag
      841 aacaccttta tctaaaagtg agagatagaa aaaagaagca aaggaatgga acaaaaggaa
      901 catgaaaaat tatatatggg actttgatgg tacattgttt gatacgtatc ccgcaatggt
      961 agatggtgca tggcaagcac taaaagattt tggtatcagt atggacaaaa aagaaatcta
     1021 cttcaaaatg aaaaagtatt ctactagtta tttgatcaat gaatcaaact tgaatgccag
     1081 agagtttaat gaattatttc accgatatga aaaagaaagt acggaagttt cccgtccatt
     1141 tccagaaacg aaacaagtac tggaaatgct aaaagacaac ggcggtcgtc atttcatcct
     1201 gacccaccgt ttgacagaat caacatgggg cctgttaaaa gaacatcgat tggcacatct
     1261 gattgaggaa gtagtaggga tcgatcagga ctttcctcgt aaacctgatc cagcatcttt
     1321 aaattatttg atcgatactt ttcatttaga aagaacagac acgatgatga tcggcgatcg
     1381 tcgtttagat atcgaagcag ggaaaaatgc aggagtcgct acttgtcttt acgacatcga
     1441 tcattttctg ggtgagatcc cggcagatta tgtggtagga aatctgaatg aaatccttac
     1501 cttgcagagc atcattaaaa agtagtatcc cctttcaaag gagatatgtt ataattttca
     1561 taaaggagtg ataaaattat atggaaatca gaagacaaaa agaagttgta gaagcatttc
     1621 attatgatat gagattgccg aaccaagaag tagaaacaga tttgaaagta ggcttctcgc
     1681 ctttaaaaga aacgatcgaa aactatccga aagaacatag catcgtagga gcacgcttag
     1741 a
//
LOCUS       DACEMO010000286         1741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.294, whole genome shotgun sequence.
ACCESSION   DACEMO010000286 DACEMO010000000
VERSION     DACEMO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.294"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(101..991)
                     /gene="pstS"
                     /locus_tag="IW728_002682"
     CDS             complement(101..991)
                     /gene="pstS"
                     /locus_tag="IW728_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1026005.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(1188..1670)
                     /locus_tag="IW728_002683"
     CDS             complement(1188..1670)
                     /locus_tag="IW728_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026006.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
BASE COUNT          505 a          363 c          297 g          576 t
ORIGIN      
        1 gaggaactat ttttcacaat taaaggaaaa atgtatatgt gggaaacata gttgattttt
       61 ccttaatagt aacgactacc gtggcatgtt tatctgctag ttatttgata tttccttcgt
      121 gatcacgttc tacttccatc attgtgattg gtaaatagcc caattctttg acaggtcctt
      181 cttggatttc ttctgtcatc atgtagtcta agaatttttg gacttcaggg gaaggttttc
      241 cgttcgtgta catatgttca tatgaccaga ttccccagct gttatctgct acattctctt
      301 cttttggttc tacaccgtcg atacttagtg cttgtgtgga atcatcgaga tatgagaaag
      361 ctaaatagct gattgctcct ggtgtttgac ttaccagctg ccgaactgtt cctgatgaat
      421 cttgttcttg ggactgcaca ggggtagcac catccagtcc ccatttttcg aatgtcgcgc
      481 gggtaccgct gccgtttgca cggttcacga cgttgatttt ttgatctttc ccgccaactt
      541 ccttccagtt agtgattttt cctgtaaaga tatcaatcaa ttcctgtttg gtgatatctt
      601 tgacatctgt atctttattg acgattggcg ccatcccgac cactgctact ttatgatcca
      661 ctaattttga agcatcaact ccgtctcttt cttctgcaaa tacatcagag tttccgatag
      721 taactgctcc agcttcgact tggctcaaac cagttccgct tcctcctcct tgtacagaga
      781 tttgataatt tggattttct tgtacaaagc tttcttgtgc tgcatcgact aacggctgta
      841 atgctgtcga acctaccgca acgattttta cttgttcatt tgagtcactt gaattatttt
      901 gtgtggcaga gccatctgta tttcctgatc ctgagccgca tccggctaaa agaattccca
      961 tcatacctag agtaaacaat acttttttca ttttattgct cctttcttat ttacaactaa
     1021 agtttatcag tcgactgtaa atccctcgta tggttgctgt aaaaatatag ttaagttatg
     1081 taaaaatcat gtaaacgttt ttcgcattct ttacataata ataaaaaaag catatgagaa
     1141 acatgcattt tacgcagctc atatgctttt ttactgatga tccaccatca atagttattt
     1201 tttatagata cagatcgttc actttttatc ctgatttctg tccagaagta aaaaaatgtt
     1261 gcaagaataa atgaggcaat tactaaatag aaccatggtt tgatcgtcaa gaaatacatg
     1321 gaggaataag ctgaatcctt caatccactc tcgctcaaac cattagggcc gataatcaag
     1381 cgaggcagca aagaaaacat agatgtaaat actaataagc aagaaaagta aagcagctgc
     1441 caattgtagt gattgatcag agattttggt aataagagac ctacacttat cactaaatag
     1501 aaaacagtca aagctggaaa attcctagaa accacaaatc cgcttccttg gatcaggcct
     1561 aatatctccg caccttgtac aacagcattt acccatgtaa aaggtagcaa aacgatagga
     1621 aaaaaccaaa aaaataaata aagccgatcg attactgctt ttcttctcat taacgttccc
     1681 ccaaactact ttttctctca tcatactata caattttcta tctaaaaaaa ttagggggaa
     1741 a
//
LOCUS       DACEMO010000287         1738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.295, whole genome shotgun sequence.
ACCESSION   DACEMO010000287 DACEMO010000000
VERSION     DACEMO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.295"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(150..1337)
                     /locus_tag="IW728_002684"
     CDS             complement(150..1337)
                     /locus_tag="IW728_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1026007.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(1430..>1738)
                     /locus_tag="IW728_002685"
     CDS             complement(1430..>1738)
                     /locus_tag="IW728_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026008.1"
                     /translation="REIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQ
                     NSDDLLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI
                     "
BASE COUNT          508 a          384 c          298 g          548 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcccgtt ttccaaatct cgcaggcgtt tttgattgct ttgatcctcc tgatccaaac
     1501 caaagaattc caaggttttc ttgatttcat gaatatcggt actgcgaaaa gcaaacttca
     1561 acccgatatt attttttaaa cgttcatcta ataaatcgtc gctattttga gtgacgaaat
     1621 agaccccagc attcatggcc cgaccagcac gaacaagctt attcgataac gtttttcctt
     1681 gagccacttg aagaaaactc caagcctcat ccaaatcgac aattttgaag atttctcg
//
LOCUS       DACEMO010000288         1733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.296, whole genome shotgun sequence.
ACCESSION   DACEMO010000288 DACEMO010000000
VERSION     DACEMO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.296"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1590
                     /locus_tag="IW728_002686"
     CDS             <1..1590
                     /locus_tag="IW728_002686"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1026009.1"
                     /translation="GAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLEQ
                     VVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLAD
                     QAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKL
                     NDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRF
                     FSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNL
                     RNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFA
                     FLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTS
                     IPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAH
                     LANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISS
                     LQTTPENEAWLQGALEVFKHE"
     gene            1583..>1733
                     /locus_tag="IW728_002687"
     CDS             1583..>1733
                     /locus_tag="IW728_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1026010.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSN"
BASE COUNT          592 a          290 c          375 g          476 t
ORIGIN      
        1 ggtgcagaat taaatcaaaa attatttagt gcggcagata atctccgtag caaaatggat
       61 gcgtcagaat ataaaaatta tttattggga ctgattttct ataaatattt atccgaccgt
      121 ttgttggaac aagtggttct tttagcagat gaatccttag aggaatatga tacggtaagc
      181 aaacaaacga tgttgtatcg cgaattactt tcagatgaag aatcaaaaga agatctgatt
      241 gcaacaatcg ttgatatttt ggggtatgcg atttcgccag agtatctttt caacgtctta
      301 gcggatcaag caaaacaagc aacgttccaa ttgaatgatc taaacaaagc ctttgtgcaa
      361 ttggcatcta cctacaatca atttaatggg ttatttgatg atgtggatct ccaatcaaaa
      421 aaactaggaa cagatgagca acaacgaaat gtgacgatta ccgaagtcat taagaaattg
      481 aatgacgtgg aagttttagg tcacgatggg gacgtgatcg gggatgctta tgaatttctg
      541 atcagtcaat ttgcttcaga agcagggaag aaagccggag aattttacac gcctcatatg
      601 gtttctgata tgatggcaca aatcgtgacg ttggatcaaa aagaacgacg attctttagt
      661 gtttttgatc caaccatggg ttcaggttca ttgatgttaa atgtgcgtaa ctacttaact
      721 cacccagata acgttaaata tcatgggcaa gaattaaata caacgacata caatctagca
      781 aaaatgaact tgattttaca tggggtagat gccgaagaaa tgaatctccg caatggggat
      841 acgttgaata aggattggcc aacagatgaa ccttacacat ttgatgcagt agtaatgaac
      901 ccaccttatt cggctaattg gtcagcagat acgaccttct tagacgattc tcggtttaat
      961 cgttatggaa aattagcgcc aaaatccaaa gcagattttg cgtttcttct tcatggtttc
     1021 tatcatttga aggaaacagg gactatggcc atcgtcttgc cacacggagt tttattccgt
     1081 ggcgctgcag aaggggtcat tcgtcaaaaa ttactagaag acggcagtat ttatgctgtg
     1141 atcggtatgc ctgcgaactt gttcttcggg acatcgattc caacgacggt gattgtattg
     1201 aaaaagaacc gccaaactcg agacgtatta ttcattgacg ctagtcgaga atttgtcaaa
     1261 ggaaaaaatc agaataaatt atcggaagaa aacattcaaa aaatcctcga aacctatgct
     1321 gaaagaaaag atgtcgagaa atacgctcat ttggcaaact ttgatgaaat caaagaaaat
     1381 gactataact taaatattcc acgctatgta gatacgtttg aagaagaaga accaattgat
     1441 atggttcatg tcggaaatga catcaagaag atacgacaag aacagcaagt gctagaaaaa
     1501 gaacttttag aagctatctc ttccctgcaa acaacacctg aaaatgaagc gtggttacaa
     1561 ggtgcgctgg aggtgttcaa acatgagtaa tgatatacaa ccagaaattc gtttcccagg
     1621 atttacggaa gcttgggaac agcgtaagtt tgaatgctta cttgataaaa aagatggagt
     1681 aaggagggga ccatttggga gtgccttaaa gaaagaattc tttgtatcta ata
//
LOCUS       DACEMO010000289         1695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.297, whole genome shotgun sequence.
ACCESSION   DACEMO010000289 DACEMO010000000
VERSION     DACEMO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.297"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..207
                     /locus_tag="IW728_002688"
     CDS             <1..207
                     /locus_tag="IW728_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026011.1"
                     /translation="RRQILCKLRKRNILMYRQLERLKHDLLRLESKRVQCELEGNQTQ
                     VEVVETKILKKKEQFLKMLTQNKK"
     gene            220..>1695
                     /locus_tag="IW728_002689"
     CDS             220..>1695
                     /locus_tag="IW728_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1026012.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDE"
BASE COUNT          485 a          273 c          391 g          546 t
ORIGIN      
        1 cgccgtcaaa tactttgtaa gttgcgtaaa agaaatatat tgatgtatcg tcagttggaa
       61 cgtctgaaac atgatctgct tcgtttggag tcaaaacgtg tacagtgtga attagagggg
      121 aaccaaacac aggttgaagt agtcgaaacc aaaattttaa agaaaaaaga acaattttta
      181 aaaatgctta cacaaaataa aaaataaagg ggatggacga tggaatttgc tgagttagtt
      241 attgtatttg cagtaacgat caccttatcg aacatagcta gccgcatttt gccaatgatt
      301 ccagctccac tgatccaaat tttccttgga gtggtattag gtctgacaga gtggggacaa
      361 tcaatcgatt ttgaacctga attgttctta gttatgatta ttgcaccatt gttatttaga
      421 gaaggagaaa aagcagatat ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg
      481 gcatttggag gagtcatttt gacccttgtc ggggtaggtg cgacgttatc attcctgttg
      541 ccaagtgtcc cacttgctgc ctgctttgct tttggagcag cgttaggtcc gacagatgcg
      601 gtagcagtca gttctctttc tggacgagtg aatatcccta aaaaggcgat gcatattttg
      661 gaaggagaag gtcttttgaa tgatgcatct ggtgttactg ccttccagtt tgctcttggt
      721 gcgctgatca ccggatcttt ttcagcagta aacgcgggga tgagtctagt agtttctagt
      781 attgggggag ctttgatcgg ctttttgctt gtctggttca aacaaaaaat aattcatttg
      841 atcgaaaaag catcagcaca agatgtcaca gggtatctgc tgatcgaact tttgcttcct
      901 tttctagcct atgtattggc agaggcattt gatgtatctg gaatcattgc tgcagtggca
      961 gcaggtattc tccaagcttc tggttttagg aaaatctctg tgtttgatgc agaattgtcg
     1021 agcttgtccc acagtacatg gacaactatt gcttttacac tgaatgcatt agtatttata
     1081 tttttaggaa tcgaattgac gcaagtgttc tcgccagtat ggggagacgg tttatatcca
     1141 aacggtcttt tattagcgat tatcgtatta atctctgtca tgctttttgt gatcagattc
     1201 atttctatca gtctttttta tgtattcaaa gatggcagta aaaagttcaa aaaacagttg
     1261 aacgagatct tgatcttaac atttggcggt gtaaagggaa cagtcagttt ggctacgatt
     1321 tttattttgc cattttcaat caataatatg atgttttacc agcggtcttt gcttttgttt
     1381 ctgacggcgg gtgtcatctt agtgactttg gtcataggga tcattgtctt gcctatgctg
     1441 acggaaacag aggaagcgaa aagcacggat ttgaatgctt tgatgatttt agaagaggtc
     1501 gttgaagttt tgcgaaaaga aataaaggaa attgatcgta atacaaaaga atttttagcc
     1561 actgaagcag tgatagaaaa ttatcaagaa cggatacgtg atctgtactt ggaagattta
     1621 accgatgatg aaaagcaaga agtacaagaa atacaagcac tgattttgtc gatcgaacgg
     1681 gatggattgg atgaa
//
LOCUS       DACEMO010000290         1688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.298, whole genome shotgun sequence.
ACCESSION   DACEMO010000290 DACEMO010000000
VERSION     DACEMO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.298"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(52..1518)
                     /locus_tag="IW728_002690"
     CDS             complement(52..1518)
                     /locus_tag="IW728_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1026013.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          535 a          336 c          282 g          535 t
ORIGIN      
        1 gtgttccaga agtagcttct gtcacaacct cttgtaacaa gtatttctat tttaaggctg
       61 aaccactgtt tctggttcag taatttcttt gtttttgtct cgacgagcaa ttgctggcaa
      121 gatcatacct aataagatca agacaattgg tgtgatgata ttcgtgaata attggaacca
      181 ccatgcttta ggatcagcag tataatctac ttttggcacc attcctagga tacatgcaaa
      241 cgcagtgaag gcaaagcacc aagcaccaaa gataaaaccg atttttggat ttttaacaaa
      301 tttatattca gaattgaatt tcttataagc tttattcaac atcatatatg ccaagaacac
      361 ccataggtaa cgcattggca tgacaactga gttcaagttc gtcaaccatt ttactaattc
      421 attcatatct ttgataccaa agatcggcaa caaaataatg attcctacta gtatacctgt
      481 cagaagataa ccattcacca atgtaccttt acgattacgt ttacgtagcc agtttggtac
      541 aaattctgga tccgcatctg ctaacaagat ttttagtggt gcatcaattg aaaaggctaa
      601 tgcggagatt tgtccaatca tgtttgtcaa tgcgtaaatg atcatcaatg agttaccgat
      661 accatagtaa ttacctaaaa tttgaaaagc actgtatgcc ccatttgcca tcaagtcttt
      721 agggatatta tcgctagcaa acagcatacc cattgcgact gagcctaata cagcacaaat
      781 ccctaccatt gctgctaaga agatcatccc tttaggaaat tcctttgcag gatttcttgt
      841 agagttgaca taaggggata ttttttccgc ccctccaact gcaaatacta gcatcgaaac
      901 ggttgtgaaa taactgaaat caaatgttgg aatatatgtt ttcacttgat tcatatgtgg
      961 tgtggcaaat tccatcgttg gattgatgaa cggtgccgtt acagctaaaa gaataaataa
     1021 gatcgacatg acgaacatcg ctgttccggc cagtccgcca atcactttca aagtagaaag
     1081 tccttttgtt gataaatata aaaaggcaag aaatataata agtgaaataa ctgctaccat
     1141 ttgtactgac atcgtattta cgatatttcc atttccttgg actgcccaac caccagcaat
     1201 cagaattgct tgaggctttt gtgccaaata aggaatatgg acgacccagt aagtccaagc
     1261 ggcatagtat gccagtcgtt tattagatgt cgtattttcg acccatgaac tgacgcctcc
     1321 tttactatcc ttgaaggctg atcctaattg tccaacgatc aatgcgtatg gaataaaata
     1381 aatggccaaa atcaatagcc aagaggtgac aactgatact ccttgctgag catagttatt
     1441 aactacattc cccatgcccc aaaccatgtt aaatgcaatt aacgcaacag taaaccagcg
     1501 taattgtttg ttgttcattc gttctctcct ttatgataaa aatttgttat ccagatgtaa
     1561 tcatattcaa tttttaacaa acttacaaga cagtgttatt ttatgtgtgt attcttttat
     1621 ttaatacaca tcgttttttc atattttttc catcaaaaaa ataagttatt ttcttttatt
     1681 tcattgac
//
LOCUS       DACEMO010000291         1684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.299, whole genome shotgun sequence.
ACCESSION   DACEMO010000291 DACEMO010000000
VERSION     DACEMO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.299"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            179..1570
                     /locus_tag="IW728_002691"
     CDS             179..1570
                     /locus_tag="IW728_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1026014.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GRISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT          479 a          287 c          374 g          544 t
ORIGIN      
        1 tcacaggtat gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat
       61 cgtctaaaat ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt
      121 ctcgggaacc tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat
      181 gacaacactc atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta
      241 tcgtatgcaa aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg
      301 tatcgtcttt ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca
      361 atcaatggaa tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt
      421 tatgccttta gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa
      481 gctagggaga attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc
      541 attgatcgga attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg
      601 agctgcagaa acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt
      661 atcgattcca cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg
      721 tagtcgtgca acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta
      781 tctaggcgtg aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag
      841 tctgtataaa atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt
      901 atttgctttg atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg
      961 taaatttgtc gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat
     1021 tttgattggt gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag
     1081 ctttgcgttt acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac
     1141 aaaagcttta ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat
     1201 tggacaaaac ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc
     1261 agctggagtg aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat
     1321 cagttctttt ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt
     1381 aggggcaatg aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat
     1441 cgatatggca cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag
     1501 tgcacgcatc aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt
     1561 gaatgcatag tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag
     1621 tttcctaaaa atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact
     1681 tctg
//
LOCUS       DACEMO010000292         1683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.300, whole genome shotgun sequence.
ACCESSION   DACEMO010000292 DACEMO010000000
VERSION     DACEMO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.300"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(352..642)
                     /locus_tag="IW728_002692"
     CDS             complement(352..642)
                     /locus_tag="IW728_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026015.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(850..1541)
                     /locus_tag="IW728_002693"
                     /pseudo
     CDS             complement(850..1541)
                     /locus_tag="IW728_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          437 a          331 c          251 g          664 t
ORIGIN      
        1 acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat
       61 cctacaattc cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc
      121 cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc
      181 ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata
      241 tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc
      301 catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat
      361 tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat
      421 tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat tattataata
      481 acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga
      541 agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg
      601 caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc
      661 attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt
      721 tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact
      781 aaaaaaaaga ggcagcagta tagattactg ttgcttcttt actaatttag gtttacttgt
      841 taagaaagat tatttacgca aatgttcttc aacaatttgc tgagatttat cagctgtatc
      901 ttccacttca tcttttgctt tttctaactt ttgaatatat tgtttcaagc ttgagttttc
      961 agcagttgct tcgtctaatt tcttttgtgt atctgttaac tgatttctac atttgttttt
     1021 tcttgtgtca gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa
     1081 agttttgctt gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca
     1141 tcattttttt ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt
     1201 tgtgcatcct tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt
     1261 ttttggtttt ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct
     1321 tgcaatgtct gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta
     1381 tctgtcaatg tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct
     1441 ccaccccaca aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga
     1501 aatgcaacga caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc
     1561 aatactttta catgctaaaa gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta
     1621 catatgtaat tttaatataa atactatcaa atactcattt aatcctcctt ttttatctat
     1681 tta
//
LOCUS       DACEMO010000293         1665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.301, whole genome shotgun sequence.
ACCESSION   DACEMO010000293 DACEMO010000000
VERSION     DACEMO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.301"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(944..1462)
                     /locus_tag="IW728_002694"
     CDS             complement(944..1462)
                     /locus_tag="IW728_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1026016.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGIF
                     RTKIRKWKRLAL"
BASE COUNT          525 a          318 c          227 g          595 t
ORIGIN      
        1 taatcgcaaa tttaccccta tactactagt ttgtaagtta gattaataaa aatactaaag
       61 ttatttgttc ttttattaaa tacacctaca acttataaat tgatggtatt tagataacac
      121 gaaagaataa aaataacagc tgatagtaaa gtttgttgac gattgctact caatcgaaag
      181 tgagttagtg cgtatgaact tgcttctgaa aacttatgaa atgaaagacc ttttacatct
      241 tgcagaaatc ggtactggga ttaaactaag caataaaaac taatgttccc ctcattcttt
      301 gaacttctac tatttgattt atttattctg ataaatgacg atttcacctt caggatcttc
      361 aaatcccgta tagaatttat ttaagatctc ttcagtatca gtaataggat aggcgatagg
      421 ccacataccc tttgatggtt agaataaact gctcaaaatc tgtatcgtat gttggcttta
      481 gcatattaac aaaggctatc tccaaattat aaaccataag cgttgaatcg ttcttaacaa
      541 tttcaattct tcctaaccca aagtcagaag atataattac cggacttgcc ttacccttca
      601 tgtgggttgt tgagattcca tatttaaagg gaattgtcgg tactatcggc ggtctgaaaa
      661 acatcccaaa aataaaaaaa tctttcccct gctagtctcc taagtccctt tgtagcggat
      721 atggcaccat aatctcattt tatcgaagtg agtcatggtt tatctgttgg ccaaccaaaa
      781 ttgatacaat ctaacgatgc accctctacg gcattctagg ggagtgcact tgcattcacc
      841 caataaaaac cggcagttag caaagaactt atgtagtcgt atgttcttac tgttaacata
      901 caagaggtct cctttccaca attgcttagc agtaaacggg aatttacaaa gctaatctct
      961 tccattttct tatctttgtt ctaaaaatcc caaatggagc aactgtattt acatgaataa
     1021 atttatatac ttcccataca gccgttttgg ttgcctcgtc tgcccatttt cttttgtgtg
     1081 cagtgaatag atcttcatca ctcattgaat caatcatcat gttaatatca tcaatattac
     1141 gattgagaat ttcctctaat tctttcaatg gtagatgtgc ataggtattt gtaaaccact
     1201 tatataactc tcctaactga ttccatttaa attgatcaga aggcgtttta acagataatc
     1261 catttctttc atcattttcc cattttagaa ttaaattcgt ccaaccaact tgatatgcta
     1321 aattttcagc aggtgttcta tccacttcct caactctttt atccttcaat tcttcaggaa
     1381 tagtttcaaa ttctaaaata tatttagtat aagtcctatt tatttctttt tttaattctt
     1441 ctttattttt atatgttctc attatattac ctccataaat tcctaattgt ctcctttaat
     1501 tcatctataa tttcatccac gatattttaa tccgttctac aatgcatatc tgatatgcat
     1561 tgactgtcta actcatatgt gaatgattta taaaatatcc caacgtctca tctaaagaaa
     1621 tgttgatcgt cagcaaggag ctaaaaaata cgaatatatt tcttt
//
LOCUS       DACEMO010000294         1663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.302, whole genome shotgun sequence.
ACCESSION   DACEMO010000294 DACEMO010000000
VERSION     DACEMO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.302"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            612..1424
                     /locus_tag="IW728_002695"
     CDS             612..1424
                     /locus_tag="IW728_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026017.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          555 a          244 c          275 g          589 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cgatgattct atctttatta att
//
LOCUS       DACEMO010000295         1648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.303, whole genome shotgun sequence.
ACCESSION   DACEMO010000295 DACEMO010000000
VERSION     DACEMO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.303"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            41..1177
                     /locus_tag="IW728_002696"
     CDS             41..1177
                     /locus_tag="IW728_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ1026018.1"
                     /translation="METTEAVQDDQDLAKMYTPGVAELSLMIEKNHALARELTISGKL
                     VAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAIPLAIEQKSVDEIVQTIV
                     NLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEGTAIVVLAGLINAAKIQR
                     KTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRLKKQDSTLNKYQRELLDH
                     IHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDNPIIFALANPVPEITPDL
                     AKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKVDLSLQLEVAKQLAAKVA
                     EPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAENIK"
     gene            1158..>1648
                     /locus_tag="IW728_002697"
     CDS             1158..>1648
                     /locus_tag="IW728_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate permease"
                     /protein_id="HAQ1026019.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFF"
BASE COUNT          556 a          246 c          329 g          517 t
ORIGIN      
        1 agttttagaa atccataaaa aaaatgtagg tgttttacgg atggaaacaa cagaagccgt
       61 gcaagatgat caagatttag caaaaatgta caccccaggt gtagcagaac taagtttaat
      121 gattgagaaa aatcatgctt tggcaagaga attaacaatt agtggtaaat tagtggccgt
      181 tattacagac gggtcggcgg tattaggtct tggaaatgta ggaaaccaag ctggattacc
      241 aatcgtcgaa gggaaagcgc tgctttataa aaatttagca ggtgtcaatg cgattccatt
      301 agccatcgaa caaaaatcag tagacgagat tgtacaaaca attgtcaatt tgcaaaatag
      361 ttttgcaggg atccatttag aagatattgc agcaccgaag tgttttgaga ttgaagaaaa
      421 attacaagaa aaactttcta ttcctgtgta tcatgacgat caagaaggaa cagcgatagt
      481 tgttttagct ggattgatca atgctgctaa gatccaaagg aaaacattaa ctgaacttcg
      541 agttttgatc aacggcatgg gagcatctgg ggttgcaaca gctagactat taatcgctgc
      601 tggtatcaaa aatctaacat tggtggataa acaagggaga ttaaagaagc aagatagtac
      661 attaaataaa tatcaaagag agttgttgga tcatatacat gatacaaata ataatgcaag
      721 cgttttggaa gatgcaatcg ttggacaaga tgtttttatc ggtctttcaa taggaaatgt
      781 tttaacgcct gaaatgattg gaaaaatgaa tgataatccg attatttttg ctttagccaa
      841 tccagtacca gagatcacac ccgatttagc aaaagaatct ggtgcaaagc taatcgctac
      901 aggttcttcg aaatatccta accaagtaaa caatatttta gtatttcccg gattgttcaa
      961 gggactatta ctatcgaaag cgaataaagt cgatctttca ttacaacttg aagtagccaa
     1021 acaattagct gctaaggtcg cagagcctac ggtagaaaaa ttcatcccta atgtttttga
     1081 tgaaggagtc gcaaacgtcg tttcacaggc agttttggat tatttacaaa aactaaaaga
     1141 aaacgaggat aatatacatg ctgaaaacat taaatagaat taaaattggt tttattcctt
     1201 tatctcttta tatcattttg gtactcgcgt atcttgcaat gacgataagc aatatcgttc
     1261 cagaaaatga tatgttagtt tctttgggta ttatgactgt ttttgcttat ttattagaag
     1321 aaattggtaa aactattcct attttaaaag atttaggtgg taaagtatta gttgttacat
     1381 ttttgcctgc atttctagtc tataaacatt ggctacctca aagtactgta actgtggttt
     1441 ctgattttat gaataataac aatttcttga tgttctttat cacactacta gttgtcggca
     1501 gtattgcttc aatgaatcgg caaacattgg ttaaagcaac ttcacgtatt atcattattt
     1561 taattttatg cgatgtgatt ggttctttga taggaacagg tgtgggaata ctccttgggc
     1621 tatctgcttg gaatgcgtat ttctttat
//
LOCUS       DACEMO010000296         1636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.304, whole genome shotgun sequence.
ACCESSION   DACEMO010000296 DACEMO010000000
VERSION     DACEMO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.304"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            146..1630
                     /gene="guaB"
                     /locus_tag="IW728_002698"
     CDS             146..1630
                     /gene="guaB"
                     /locus_tag="IW728_002698"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1026020.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          563 a          284 c          378 g          411 t
ORIGIN      
        1 acaatctctt ttaatataca cagatgtaaa cgctttatat tggtattcta tgagaaagct
       61 aacttgattc caagtagatc attgacttaa atgaaaaaat caattatttt aatagataaa
      121 ctaaattaga ggagtggtac taaaaatgtc caactgggaa acaaagttca cgaaaaaagg
      181 atttactttt gatgatgtct tattgatacc agcagaaagt cacgtgttac caaacgaagt
      241 ggatatgagc gtacaattag ctaaaaatat taagttgaat attccaatca tcagtgcaag
      301 tatggatact gttaccgaca gcaagatggc cattgctatg gcaagacaag gcggactagg
      361 tgtgatccat aaaaacatga cgatcagcca gcaagcggat gaagtaagaa aagtcaaacg
      421 ttctgaaagt ggcgtaatca ttgatccgtt cttcttaacg ccacagcatc ttgtagcaga
      481 cgcagaagaa ttgatgagca aatatcgtat cagtggtgtg ccaatcgttg aaacacttga
      541 aaacagaaaa ttggtaggga taattacaaa ccgcgacatg cgttttgtaa cagattatca
      601 catgccgatt gctgatgtga tgacaaaaga caacttagtg actgccccag taggtacctc
      661 tctaaaagat gcagaaaaga ttttgcaaaa acataaaatc gaaaaattac caatcgtaga
      721 taacgaaggt cgtttaagcg gattgatcac gatcaaagac atcgagaaag tgatcgaatt
      781 tccaaatgca gcaaaagatg aacatggtcg attgcttgta gcagcagccg ttggtgtaac
      841 aagcgataca tttgagcgtg cgaatgcttt attagaagca ggggcagatg caatcatcat
      901 cgatacagct catggacata gtgcaggtgt gatccgaaaa attcaagaaa tccgttcaac
      961 ttttgcagat gcaacattga ttgcaggaaa cgttgcgacg gctgaagcga caaaagcttt
     1021 atatgatgca ggtgtagatg tcgtaaaagt tggtatcgga ccaggatcga tttgtacgac
     1081 acgtgtggta gccggtgtag gtgtacctca gctcacagct atttatgatg cagcatcagt
     1141 tgctcgtcaa tatggcaaag cgattattgc agacggaggg atcaaatatt ctggtgacat
     1201 cgtcaaagca ttagctgccg gcggacatgc agtgatgcta ggaagtatgt tggcagggac
     1261 agatgaatca ccaggtgaat tcgaaatctt ccaaggacgt cgattcaaaa cataccgagg
     1321 tatgggctca ttaggcgcga tggaaaaagg ttcaaaagac cgttacttcc aaggtagtgt
     1381 caatgaagca aataaattag taccagaagg aatcgaaggt cgggttgctt ataaaggaag
     1441 tgttgcagat attattttcc aaatgatcgg cggtttgcgt tcaggaatgg gttatgttgg
     1501 ggcagccaat ctacagcaat tacgtgatga agcgcaattt atccaaatga gcggcagtgg
     1561 attaaaagaa tcacaccctc atgatgtaca aatcacaaaa gaagcaccaa actattcagt
     1621 tgaatcttaa aaacat
//
LOCUS       DACEMO010000297         1634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.305, whole genome shotgun sequence.
ACCESSION   DACEMO010000297 DACEMO010000000
VERSION     DACEMO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.305"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            190..546
                     /locus_tag="IW728_002699"
     CDS             190..546
                     /locus_tag="IW728_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1026021.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            556..1455
                     /locus_tag="IW728_002700"
     CDS             556..1455
                     /locus_tag="IW728_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ1026022.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          538 a          287 c          342 g          467 t
ORIGIN      
        1 aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc
       61 tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata aattgagaac
      121 agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct atgacatgag
      181 gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa gaataaatta
      241 gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt tgcagaaagc
      301 caagtcagtt gccaattgga tacggcattt cgattgatca ttgtcaaaaa agatacacaa
      361 acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat caaaatcaaa
      421 gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc aacttataat
      481 agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa
      541 gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt ggacatcaat
      601 aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca caagatcact
      661 cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc tgttgtcgaa
      721 tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga gttggcagat
      781 gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa tgaacggtat
      841 attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga ctatcagttt
      901 tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg
      961 ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa
     1021 aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt
     1081 cctaaacggg catttatcat gtatcaatct gctccttatg agcgttggaa caaattaaat
     1141 gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa tatcaaacaa
     1201 gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca cacgatttac
     1261 agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt tgtcttgaaa
     1321 gaacagttgc tagacagcta tgatcctatg aaattacgtg tcctcttgcg tgcccatcac
     1381 ccagcttttc aggcctatca aacacaacat ttgcaagagg aatttgaaca agcgatgaca
     1441 atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg taaaacataa
     1501 ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt gcggaaaata
     1561 atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag atgcaagcgt
     1621 gtattctttt tttg
//
LOCUS       DACEMO010000298         1586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.306, whole genome shotgun sequence.
ACCESSION   DACEMO010000298 DACEMO010000000
VERSION     DACEMO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.306"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..619
                     /locus_tag="IW728_002701"
     CDS             <1..619
                     /locus_tag="IW728_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1026023.1"
                     /translation="TRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTEQDIS
                     TDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLRSFLS
                     EASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKTLIYS
                     ISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            797..>1586
                     /locus_tag="IW728_002702"
     CDS             797..>1586
                     /locus_tag="IW728_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1026024.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHP"
BASE COUNT          502 a          316 c          268 g          500 t
ORIGIN      
        1 tactcgcatt cctgatttta caatccaaaa cgggcagctt caaaccgaag aaaaagaagg
       61 attcatttac caaacaaact ccattatctt cacatttgat ccagagggaa aacgaacaga
      121 acaggacatc tctactgatt tgatggggaa ttttgttagt gtcgggatgt taaaaaacaa
      181 acttatcata gctcttccta atacaggcac gacttctgct ttgttgaaca ataaccaatt
      241 agaacttccc tacaccaatg aatctttaaa aaacttaaca ggtaagcagc tccgttcttt
      301 tttaagtgaa gcatctatac cactttggat aaaagtcctg acatttatat tttctattta
      361 tcctagtttt ctgaatctgc tgatcacttt gctatttacc aacattgctg cttttctcta
      421 tgcacgcttt cgactaacaa aagctacttt tcttgattgt ttaaaaacat tgatctattc
      481 aatttcattg cctactataa ttgcaacaat cttgatggtc tttcttcctt cttttgacac
      541 tagcgccttc attgcttttg caggaatctt tatttttgct caagctgtca aaggatgggc
      601 aaaaatatcg atcaattaag aaataaagac aactcgttat ttctacttca atagaccctt
      661 ttagaggttg tgacattttt ttgtcacagc ccctttttta tttgaaaaat atttctttct
      721 caaaaaacaa gcaaggcgct tcaaagttct tcttgtatgc gctacactta gagtaaagaa
      781 aggcgggaaa ttaactatgt cagaaagaat catttatgca gatcgtccat ttcgtgatga
      841 atttatcgaa gaaatccata ctatagcgcc tggttatatc tttaaaacag aattacaacc
      901 agaggatcta caaaatgtag agatcagttt gggctggaac tcagactacc aagaaaaact
      961 attagcctct tctaccttga aatgggtcca atctatctca gctggtgttg atcagctacc
     1021 ccttgagact ttttcaaaga aacatatttt actttcaaat ggtagtggca tccacagtga
     1081 atcgattacg gagcatgtct taggaatcat ccttgggtat tctcgtggtc tttttcaagc
     1141 acaaaaggca cagagtgaca tgaaatggtt aggttcatcg gtccattacc aatctatcca
     1201 agggaaaaaa atactaatca ttggaactgg ccatattgga aaaatgattg caaaaaaatg
     1261 caaagcatta gacatgaact gctatggaat caatacatcc ggtcatccag cagaaggaat
     1321 ggaaaagact ttttctttaa atcaactaaa ggaagttctt cctgaagtag atttcgtagt
     1381 caatatctta cctctaacgg aggaaacaac tgggctcttt gatcagcata tgtttgaaca
     1441 ctttgatcca aaatctgtat tcatcaatgt tggccgtggt gcttctgtta agacacaaga
     1501 tctggtgcag gctttaaata accagcaact ttcctttgca gcattagacg tttttgagga
     1561 agagcctctt ccacaagatc atcctc
//
LOCUS       DACEMO010000299         1534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.307, whole genome shotgun sequence.
ACCESSION   DACEMO010000299 DACEMO010000000
VERSION     DACEMO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.307"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(52..1518)
                     /locus_tag="IW728_002703"
     CDS             complement(52..1518)
                     /locus_tag="IW728_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1026025.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          501 a          325 c          289 g          419 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aatt
//
LOCUS       DACEMO010000300         1526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.308, whole genome shotgun sequence.
ACCESSION   DACEMO010000300 DACEMO010000000
VERSION     DACEMO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.308"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..734)
                     /locus_tag="IW728_002704"
     CDS             complement(<1..734)
                     /locus_tag="IW728_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1026026.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWH"
     gene            complement(731..1120)
                     /locus_tag="IW728_002705"
     CDS             complement(731..1120)
                     /locus_tag="IW728_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1026027.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(1220..1360)
                     /locus_tag="IW728_002706"
     CDS             complement(1220..1360)
                     /locus_tag="IW728_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1026028.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
BASE COUNT          414 a          328 c          245 g          539 t
ORIGIN      
        1 atgtgccatc cagcgacgtg taagttcgca ttttcgattt ctccaaactg atcaatgtga
       61 gaatggttcg ttccatctga taaagttgga tttgctgcac ctgctcgggt tggatcaatg
      121 acaggcttgt tctcagaagt cgttgggttt tcgtctgtaa atccatgagc taaatcgtaa
      181 gccagttttt ccttacttac acccatttct gataagtatc catacggatc agtgtgatcg
      241 ccccaaatgt tttgagttac ccataaatgt gatttgattc ctggttgatt atacggtgtg
      301 tctaatgtaa gtggaatacc gtatttatta gcggaatctc tcgctaattc aacatatgcc
      361 ttgtagttct tttcaaacgt tactttatcg tgcgtatgtt gcagttcgat ttgcacagga
      421 ctatttgcat tagcatatga accagcgcca tattgtacat aaccaggttg tccaacttgg
      481 taaacgatcc caccatcgcc aacaatatac gctgtatagg cattgtacca agaacgtttc
      541 atgtactgcg cttcatttcg tcctgtcgca gtttcgtttg ctgtttcatg caataggatg
      601 tattggttat ttgctacttg agaactacct tcgtttgcgc ccaaattgaa ttcattgttg
      661 atcgagtaag caaaggcgtt tattggcaat aaaaaaagag ctataatagc tcctacaaag
      721 attttctttt tcatttggtt cctcctcggt ctttattttc agtatcattt cgtatttgtt
      781 ggaatacctc ttttaactta tctggcaaag gaaggaatac cgaaatgttt tctaaaaaac
      841 taatcccttc atttgcaaca tagaacataa taactatttc tcttagaggt atagaattcc
      901 caacgatttt ctgtacgtca accgcaacgg caatcacaac gaagattaag actttcttca
      961 acaatccttc gaaacctatc tcactactta tttctttcaa tttccatgcc ttagcaagtc
     1021 cagttaagaa gtccacaccc acaagaaaaa ttaaaacatg tagtatattg tccatccctc
     1081 ccaataaacc aacaattgtc ccgcctatca cccctgtcat tactgagagt gtatttaaat
     1141 atttttccaa tatctatttc tcctttatgc aaagaagcgc actcgtttga gtacgctttt
     1201 tatttaactt tgtgcttaat taaatttcat ttccagtaat ctgcttatat tggtcttttg
     1261 ttaaacagtt catgtttaca tagtcaagta aatcttggtc cgtataacat ccccaatcat
     1321 aaaattgttt tatattttca aagcccggaa aactattcat tacttttccc tccattctta
     1381 atacttgcga cttccatcgt caattgagct aacatttgtt gcattttttc ttgttcctgt
     1441 accgatatcg ccaattgttt tgtcagtaga gcatttaatt gcttcaactg atcgacatct
     1501 tcaatatcgt caaccgctgt gccact
//
LOCUS       DACEMO010000301         1525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.309, whole genome shotgun sequence.
ACCESSION   DACEMO010000301 DACEMO010000000
VERSION     DACEMO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.309"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..922
                     /locus_tag="IW728_002707"
     CDS             <1..922
                     /locus_tag="IW728_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1026029.1"
                     /translation="TRSQRRKKLAQMIQERIKEELGLIAAVGVGDNPLLAKLALDNEA
                     KNNEGFIAEWTYQNVPDKVWNIPEMTDFWGIGSRMKKRLNQMGIMSIKDLANWNPYTI
                     KSRLGVIGLQLYFHANGIDRTDIAIPPEPSKEKSYGNSQVLPRDYTRRNEIELVVKEM
                     AEQVAIRIRQHHCKTGCVHLHIGTSILETRPGFSHQMKIPITDNTRELQDYCLFLFNK
                     YYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEKLDKIIDKIRQKYGFTSIIH
                     ASSKLEGARSITRSTLVGGHAGGVGGLKND"
     gene            915..1265
                     /locus_tag="IW728_002708"
     CDS             915..1265
                     /locus_tag="IW728_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026030.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDAENEQRANQNLAKEQMTTE
                     EITEVLNAAVIKNRSVCIQKEERNAEGYYPDDIIGKIFGSDELRIYVGKNKVHYDEIR
                     HIEFYQKHKWSCLE"
BASE COUNT          571 a          230 c          308 g          416 t
ORIGIN      
        1 aacacgaagt cagcgaagga agaagctcgc tcaaatgatc caagaacgta ttaaagaaga
       61 gctaggattg attgctgcag taggtgtcgg agataatcct ttgttagcca aacttgcctt
      121 agacaacgaa gcgaagaaca atgaaggatt tattgccgaa tggacgtatc aaaatgttcc
      181 tgataaagta tggaatattc ctgaaatgac agacttttgg ggtatcggtt ctcgaatgaa
      241 aaaaaggctc aaccaaatgg ggatcatgag tatcaaagat ttagctaatt ggaatccata
      301 caccattaag agccgtttag gcgtgattgg gttacagctg tacttccatg caaatggtat
      361 tgatcgaact gacatagcaa tcccaccaga accgtcaaag gaaaaatctt atggcaattc
      421 acaagtgtta ccacgagatt atactagaag aaatgaaatc gaactggtcg taaaagaaat
      481 ggctgagcaa gtagcaatcc gtattaggca acaccactgt aaaacaggtt gtgttcattt
      541 acacattgga acgtcgatct tagaaacgag accagggttt tcacaccaga tgaaaatacc
      601 tatcacagat aatacaagag agctacaaga ctattgtctg ttcttattta ataaatacta
      661 tgaggggcaa gaagtccgac acgtcggaat aacctatagc aagctagtat acactgattc
      721 gcttcagtta gatttgttta gtgatccgca aaaacagatt gatgacgaaa aactagataa
      781 aataatagat aaaattagac aaaaatatgg ttttacgtca attatacacg cttcttctaa
      841 gcttgaaggt gctagaagta ttacaagaag cacgttagtt ggtggtcatg caggcggcgt
      901 aggagggctt aaaaatgatt gattacaatg atcgtggacg tttaaagtgg gcaggattct
      961 atttatcaga tcatactgaa gaaatcgatg ctgagaatga acagcgagcc aatcaaaact
     1021 tagcaaaaga acaaatgaca actgaagaga ttacagaagt attgaacgct gcagtcataa
     1081 aaaatagatc agtatgtatt caaaaagaag aacgaaatgc tgaaggatac tatccagatg
     1141 atattatcgg taaaattttt ggaagtgatg agctaagaat ctatgtcgga aaaaacaaag
     1201 ttcactacga tgaaatacgt catattgaat tttaccagaa acataaatgg agctgtttag
     1261 aatgaacatt ataaagcaat atgagcttaa gtatataaca cttgaaaatc tttcagatga
     1321 aatctgggga tatggtcaac aactaattaa tgaaatagga atcgactgtt ttaagtttta
     1381 cgtattaata gctggtggat acttaaattt tgaatatcat atattgaatt atagtgattc
     1441 tcacttttcg gtataaataa aaaggataga aaaacttcta tcctttttat agttttataa
     1501 acttataaat tcataatttt ataag
//
LOCUS       DACEMO010000302         1519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.310, whole genome shotgun sequence.
ACCESSION   DACEMO010000302 DACEMO010000000
VERSION     DACEMO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.310"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1469
                     /locus_tag="IW728_002709"
     CDS             <1..1469
                     /locus_tag="IW728_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1026031.1"
                     /translation="LTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNLFSDEEIS
                     VSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGR
                     TKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVA
                     ETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQ
                     EEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFT
                     GYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVL
                     EKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEP
                     IFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPV
                     KYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          509 a          273 c          317 g          420 t
ORIGIN      
        1 atcttacaac tgaaatccaa ctattgaatg aaaaggtcaa ttatttaacc aacaaattat
       61 ttggacgctc taaagagact ctctttgagg aaacgaatgg gcaattgaat ctttttagtg
      121 atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca gtaaaaggac
      181 atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata acagagaaag
      241 aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag atgaaagata
      301 tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg gattgtctca
      361 cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt tcttctttta
      421 aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat agtttagtgg
      481 cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa gagaactatt
      541 ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg catattaaag
      601 cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt aatcaagaag
      661 agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa acagatacct
      721 attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat tatcaccacg
      781 acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca gggtatttac
      841 actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc tattgtttcg
      901 cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac acggatattc
      961 ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag aaatggaaag
     1021 acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca ttatttattg
     1081 ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg gatgcagccg
     1141 ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat ggtcgtttag
     1201 aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga cggaaaaatt
     1261 ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg agtgtatata
     1321 aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta tttgataaga
     1381 taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct tggaacaagg
     1441 atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt ctattctata
     1501 ggtcgggtta tttgacgta
//
LOCUS       DACEMO010000303         1502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.311, whole genome shotgun sequence.
ACCESSION   DACEMO010000303 DACEMO010000000
VERSION     DACEMO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.311"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(68..>1502)
                     /locus_tag="IW728_002710"
     CDS             complement(68..>1502)
                     /locus_tag="IW728_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1026032.1"
                     /translation="LLARYLEFEKKNYVVLPKLYETLGLSKFKTRNYIIELNKEFTEF
                     KTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTEIVDSKVTIEKFAENH
                     FLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFGVYFGVFNGFSMPFRE
                     SVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGNQPIGKRFFSDEYFKS
                     SEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELDGAFGLAEAHFHDIHM
                     ISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNWIFDFEMTSFTSKKQL
                     QFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFLLVGICPVSYFEAPIH
                     VCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSDCLLEKFNKEQIIWKN
                     PPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          486 a          268 c          212 g          536 t
ORIGIN      
        1 accttttctt tgaaaaaaac ataaatcagc taataaatat gtgagacaaa gctgccatcc
       61 ataaatacta gctgattttt tcagttaaat gtctattttt ttctctgatg atggtatttc
      121 caaaatattc ccagtcatct ggagtcggtg gatttttcca aattatctgt tctttattaa
      181 atttttcaag taaacaatca gataaataaa tattcgtacg agaagtgatt cgactctcaa
      241 tcacaatatt gtaattttta aatcctttca cttgagaaat aatgtaatcg gtataagcag
      301 atccttgcga aaaatcaata cagacatgaa tcggtgcttc aaaatatgat accggacaaa
      361 taccgaccaa taaaaagaga taatcataaa ataagcgaat catgaaacgt tcatcttttc
      421 ccaagaaata gcgtttattc tcttgtatga ccttttggat tgctgtactg aatattggat
      481 acgtctcgaa gaaaaactgt agctgctttt tagacgtaaa gctagtcatc tcaaagtcga
      541 atatccaatt ttttcgattg acatgcatca tacccatacg aagttctttt cgtatatata
      601 gtctttcttc ttcagagatt tcaatcgaaa atggtaattg atcaatcaat tccactgaca
      661 ttttttgact aatcatgtgg atatcatgaa aatgagcttc cgcaagtcca aatgcgccat
      721 ctaattcaaa ttcttcttcc gtgatcaaaa acaacatgat ataagaaatc tcatcaatga
      781 tctgctcttt agggatatga agaaaagaaa tatgtgataa atccttgatg atcttcattc
      841 cttctgagct cttaaagtat tcatctgaaa agaaacgttt tccgattggt tgattcccat
      901 aggccctcaa caaactaata gtggttagca aattgaattt gattcgtttt gttgcagaca
      961 gttttaaatt gaacagatat tcaaaatatt gcacgatact ctttgcttca ttcatgacgc
     1021 tttctctaaa tggcatagaa aaaccattaa agaccccgaa atatacccca aataatacgt
     1081 tccgtatgtt tatttcttcc ccaactaata tgtttttctt taactttatt tgatgctgtt
     1141 caagaaaaga gactaattct cttcttttta cataggctct tgatatgctt aaaaaatgat
     1201 tctctgcaaa tttttcgatc gttacttttg aatccacgat ctccgttaac agtcgagcaa
     1261 tttttgatcg ttgaaagtag attcttctta aatttttgat atccgtcaaa ttcagacgtt
     1321 cgacatggat ctctgcttct tctgaaattt ggatctctgg ctttgttttg aattcagtaa
     1381 attctttatt taattctatg atataatttc ttgttttaaa tttagacaaa cctaatgttt
     1441 catataactt aggcagcact acataattct ttttctcaaa ttctaaatat ctagctagca
     1501 gc
//
LOCUS       DACEMO010000304         1499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.312, whole genome shotgun sequence.
ACCESSION   DACEMO010000304 DACEMO010000000
VERSION     DACEMO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.312"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(53..787)
                     /locus_tag="IW728_002711"
     CDS             complement(53..787)
                     /locus_tag="IW728_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1026033.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..>1499
                     /locus_tag="IW728_002712"
     CDS             1114..>1499
                     /locus_tag="IW728_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1026034.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVN"
BASE COUNT          490 a          271 c          289 g          449 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacga
//
LOCUS       DACEMO010000305         1498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.313, whole genome shotgun sequence.
ACCESSION   DACEMO010000305 DACEMO010000000
VERSION     DACEMO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.313"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(117..1226)
                     /gene="rpoD"
                     /locus_tag="IW728_002713"
     CDS             complement(117..1226)
                     /gene="rpoD"
                     /locus_tag="IW728_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1026035.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1250..>1498)
                     /locus_tag="IW728_002714"
     CDS             complement(1250..>1498)
                     /locus_tag="IW728_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1026036.1"
                     /translation="DFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDVISKSGLADE
                     ITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
BASE COUNT          359 a          336 c          269 g          534 t
ORIGIN      
        1 gaatgaattt ccttcctata gaaaaaaaag cttgagactg cgtagtctca agcttttttt
       61 cttttaaaat aaaccaaaca gttttactga tttctcttct ggtcaatagt ccacgcttat
      121 tctaagaaat cttttaattg ttttgaacga gaaggatggc gcaattttct taatgcttta
      181 gcctcgatct gacgaatacg ttcccgagta acaccaaata ctttccctac ttcttccaat
      241 gttctcgtcc ggccatcatc taaaccaaaa cgtaagcgaa ggacattctc ttcccgatca
      301 gtcaatgtat ctagtacatc ttccaattgt tcttttagta actcataagc tgcgtgttct
      361 gctggacttg ttgcttcctg gtcttctatg aaatcaccta agtgagagtc atcttcttca
      421 ccgattggcg tttcaaggga aacaggttct tgcgcgattt tcaagatttc tcggactttt
      481 tctgttggaa gatccatttc cgcaccgatt tcttctggcg taggttctct tccaaggtct
      541 tgcagcaatt gacgttggat acgtatcaat ttattaattg tttcaaccat atgaactgga
      601 atacggatcg tacgtgcttg gtcagcgatt gctcgagtga tggcttgacg aatccaccat
      661 gtagcatagg ttgaaaactt aaatcctttg cggtagtcaa atttttcaac tgccttcatc
      721 agtcccatgt ttccttcttg gatcaagtca aggaactgca tgcctcgtcc tacataacgt
      781 ttagcgatac ttactactaa acgaaggttt gcttctgcca gacgctgttt cgcttcttgg
      841 tcgccttctt cgattttcaa tgctaacgct acttcttctt ccgcagttaa aagggagaca
      901 cggccaattt ctttcagata catacgaacc gggtcattga tttttacacc ggttggcgca
      961 gaaagatctt ctgtttttgc ttgttcagct tttttctcag cactcttcaa gctgtgaatt
     1021 gatggatcac cattttcatc caccacactg atacctgcgt cttcaatctt ttggatcaat
     1081 ttgtccattt catctgcatt caaagtgaat ggtgtagcaa gctgattgga taaatcatca
     1141 tatacaactt gtcgtttggg tttgttttct ttgatgaaag ctgctactgc tgcttcatac
     1201 gtttttttat ctgttccatt tgccataaaa gaaggccccc cttgagtagc tatttggctt
     1261 gttttaactg tttcgttaaa ttgatgattt caatcgtcaa ttcaagctca agctgtttgt
     1321 ttccagttcg tctggcttct tgctgcattt gtttttttaa tgttatttca tcagctaagc
     1381 cagatttgct gatcacatct agcaagtctt ggatttctcg tggcgaactt tcttctgcca
     1441 ccggtaaata ttcgatttcc accaccaacc gccttaaatt ttcatcttgc aaaaagtc
//
LOCUS       DACEMO010000306         1484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.314, whole genome shotgun sequence.
ACCESSION   DACEMO010000306 DACEMO010000000
VERSION     DACEMO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.314"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..1263
                     /locus_tag="IW728_002715"
     CDS             22..1263
                     /locus_tag="IW728_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1026037.1"
                     /translation="MNAYQPESVDDMQDALKDVFGPLFEKMLQGELNNHLGYDAHSKE
                     PKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIPKRKRDVSDIEGKVLSMY
                     ARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQARPLAKCYAFLFVDCMY
                     VTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNRWMQVFDELKARGVEDVF
                     FISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSKDYKEVCRDMKKFYGASS
                     LNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSAIRKIMYTTNAVESVHSS
                     FRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAMVLNQLMINETFSSRMKS
                     MRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          464 a          261 c          307 g          452 t
ORIGIN      
        1 gaaaagttag ctgaatccat tctgaatgcc tatcaacctg aatctgtcga tgacatgcaa
       61 gatgctttga aagatgtgtt tgggcccctt tttgaaaaaa tgcttcaagg agaattgaat
      121 aatcatttag gttatgatgc ccattctaaa gagcctaagg aacacgataa ccgtcgaaat
      181 ggctatggaa ctaaaacgct taaaaccagt tttggtgaag tagctattga tgttcctaga
      241 gaccgggaag cttcctttga accagagtta attcctaaga gaaaacgaga tgtctccgac
      301 atcgaaggga aggttctttc catgtatgca cgaggaatga gtcaacgaga tattgccgca
      361 accgtcgaag ctatttatgg ctttgatatt tcccatgaaa tgatttcaga tatcactgac
      421 gctgtccttc ctgaattgga agaatggcaa gcccgccccc tagcaaagtg ttatgccttt
      481 ctattcgttg attgtatgta tgttacttta cgagaaaatt atgaagccaa agaatatgct
      541 gtatacacca ttcttggcta tgatctcaaa ggaaataagg agattttagg attatggtta
      601 aatcaaacag aatctaaaaa tcgctggatg caagtatttg atgaactgaa agcacgtggt
      661 gtcgaagatg tctttttcat ttcgatggac ggtgtttctg gcctcgaaga aggcgcaaaa
      721 gcgatctttc catcggtaat tgttcagcgc tgtattgttc atcttgttcg aaatgcattg
      781 cgctatattc caagtaagga ctataaggaa gtctgccgag atatgaaaaa gttctacggt
      841 gcttcttctc taaacgctgc acatgctgct tttgacagtt ttcaaaatcg gtggtctcat
      901 tattctggtg ctgtagatgt ctggaaacgg aactttgcac atgttgaaca actatttgat
      961 tatggtagtg ccattcgaaa aattatgtac actaccaatg cggttgaaag tgtccactcc
     1021 agcttcagaa aagtcacaaa aaaaggagcg ttctctaatg agaatgcact cctaaaacta
     1081 ctttatttac gcacgaaaga attacacgcg aagtggtcag gtggtcgaat tcaaaactgg
     1141 gctatggtgc ttaatcagtt aatgatcaac gagaccttct cctctagaat gaaaagtatg
     1201 cgatttacct tccgtaacaa cggtaggttt attcaactat gtgtccgatc aaaatttatc
     1261 tgatttcaaa atagccgaga gaattatggt ggttaaaaac tcttaactat ttaattacgg
     1321 ttttcatagt ttttggattc aggaggatta aaacgaattc tataaaaaaa gatttggttt
     1381 aaatgaatga ttaaacgaca atttagcaac gtagtcagag taaaacagct ctttcttttc
     1441 gtatttttta aagttgttac gaaaaagttg ctataaatcc atct
//
LOCUS       DACEMO010000307         1475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.315, whole genome shotgun sequence.
ACCESSION   DACEMO010000307 DACEMO010000000
VERSION     DACEMO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.315"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            43..1269
                     /locus_tag="IW728_002716"
     CDS             43..1269
                     /locus_tag="IW728_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1026038.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(1377..>1475)
                     /locus_tag="IW728_002717"
     CDS             complement(1377..>1475)
                     /locus_tag="IW728_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1026039.1"
                     /translation="WLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT          537 a          229 c          314 g          395 t
ORIGIN      
        1 atatgagaca atattttaga aaatgtgaaa cagaggtgta ttatgaagcg atggcaaaaa
       61 gttctattaa ccttgttagg ggtattcgtt ttgctgattg ccggattatc agcatacgga
      121 atcagattta tgggagaagc gagccaaaca gtaaacaaaa tttctaaaaa ctctaatcga
      181 atatcctcca aacgttctga gagagtaagc attgatgaca aagaaccttt ttcggttttg
      241 ctattaggtt tagataccgg tggattagga agaacggaac aaggacgttc tgatacgatg
      301 atggtagtaa cagttaatcc gaaacaaaaa aaatcaacga tcatcagttt ggaccgagat
      361 atttatacga atatcgtagg ttatggaacg gtagataaat taaatcatgc ctacgcattt
      421 ggtggtgtcg aaatggccat ggattcaatt gagcaactgc tcgatattcc tattgatcat
      481 tatgtaacga tcaatttgga tggcatggaa gatttgatca atgcggtcgg tggcatcaaa
      541 gtgaataata aaattgactt tactttggat ggtgtccatg taccagtagg tgaacaaaca
      601 ttgaatggag aaaaaggttt agcctattct cgaatgcgac acgaagatcc agaaggcgat
      661 atcggtcgac aacgtcgtca gcgggaagta gtgacgaaaa tcgtcaataa agttttaagt
      721 ttagatggcg taagcaatta tagaaaaatt ttaaaagcag ttgagaacaa tgtgacgacg
      781 gacttagatt gggatgacat gcttgatgta gcgacgaact acaccccggc gtttgaaaca
      841 atcaaacaag accagttgca aggcgaagga caaatgatca atgatatcta ctatcaaatt
      901 ttaggaaaaa atgatcttct ttcgatccaa aatgaactga agaaacaatt aaatataaaa
      961 acaagcgata cattacctaa tttgaaaaat gagaatgctt caatgatgtt ctttgatgat
     1021 tcggaagatg gcgattcgac aaacggtgat ggatcgaata gtgaagggta caccgattat
     1081 tcttcctatt cagaaaacac atatgatcag taccaagatc cgaataccta ttatcagcaa
     1141 acagaagata caagtcagta ctatacacca agtcaagata ctcaaagtta tgatccttac
     1201 gctggacagg aacaaaatgg ttatggttat gaccagaata atgagtatgg aacaacaagt
     1261 gggtactaga aaaaaatagt ttggttaata aagagtgcca cctaaattag acaaaaaatc
     1321 taacttaggt ggcacttttt ctatgtgtta aattacagga cgtctctttt tagcgcttag
     1381 tttaaaaaat aaattgaata attcaataga gttgcgaata gtacccataa ataataagga
     1441 atcatgagcc acattgccag gcgatgatgc agcca
//
LOCUS       DACEMO010000308         1464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.316, whole genome shotgun sequence.
ACCESSION   DACEMO010000308 DACEMO010000000
VERSION     DACEMO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.316"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            43..1410
                     /gene="nhaC"
                     /locus_tag="IW728_002718"
     CDS             43..1410
                     /gene="nhaC"
                     /locus_tag="IW728_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1026040.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT          380 a          280 c          325 g          479 t
ORIGIN      
        1 ttttcatggc agggcctttt catttgaaat aggagatcga cgatgaataa aaatatttct
       61 ttaaaagaaa gtatcgctgt attgatcgtg ttgttggcac tcctaggagt actgatcatc
      121 ggattccagc tctcgccaca tatcccgatc cttgcagctt tcatgtgttt gttgttttat
      181 ggcagattta aaaagttcac atgggatgag atccatgacg ggatcatcaa aggaatcact
      241 ccagggatca ttccaatcct gatttttctt ttgattggtg tcttagttgc ttcatggatt
      301 ttatctggaa ccatccctac gattatggtt tatggattta aacttgtttc tgtgcgtttc
      361 tttttaccga cagcatttct tgtttgtgca attgtcggag tgactgtcgg cagttcattt
      421 acaacgattt cgacgattgg aattgctttt ttggggatcg gtcatttact tggattcaat
      481 gatgcaatga ctacaggcgc agtcgtttcc ggtgcctttt tagggaataa ctgctcgcct
      541 ttatcagata cgaccaactt ggcagcgggt attggaggag ttaatctatt cgaacatatc
      601 gctggtatgc gttatacgga tttcccagca ttcgtccttt cgttgatttt ttatacgata
      661 ttaggacata acagtcattc agccgatcta gctagtatca atgaaatgat ccaaaacatg
      721 aaagcgagct tttggatctc tccttggaca ttgattccac tagttatttt atttgggggg
      781 gcaatcaaaa aaattccggc tatccctaca ttactagcgg gatcgacagc tgctctcgtt
      841 ctttctttta tccatgagaa aagtttgacg gtcggaaaag cagcagacat cttgatgaat
      901 ggttatgtcg caaatacacc tgatccaaag ctagatgaac tgctttctcg aggcgggatc
      961 atgagtatgt taggttctgc tagtttgatt ttacttgcat tggcattagg tgggttactg
     1021 atcaaatatc tgatcgttga taccatcgtt caagaattaa aagaaaagat ggaccgacct
     1081 tctcgtttag tcgggtttac tgccttgagc tgtatcggaa tcaatctgat cgttggagaa
     1141 cagtatctgt ccatcattct tccaggagaa acattcaagc gttcttttga tgacgttggc
     1201 ttatcaaaaa cctatttgac acgaacacta gcggattcag gtgcgaccgt caattctctc
     1261 gtaccgtggg gagtcagcgg cacattcatt atgggaacga tgaaagttag cgcgttgcat
     1321 tacttgccat tcgccttttt ctcgattctt gcacctgtct ttacaatatt aggcggtttt
     1381 ttattgaatc gtcgacgtgt aaaaaattga aggaaggaaa aaagaggctg ggacagaagc
     1441 gtttaactcc aagaaataag aaga
//
LOCUS       DACEMO010000309         1442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.317, whole genome shotgun sequence.
ACCESSION   DACEMO010000309 DACEMO010000000
VERSION     DACEMO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.317"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(605..862)
                     /locus_tag="IW728_002719"
     CDS             complement(605..862)
                     /locus_tag="IW728_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1026041.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(855..1124)
                     /locus_tag="IW728_002720"
     CDS             complement(855..1124)
                     /locus_tag="IW728_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1026042.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(1255..1434)
                     /locus_tag="IW728_002721"
     CDS             complement(1255..1434)
                     /locus_tag="IW728_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026043.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT          458 a          259 c          160 g          565 t
ORIGIN      
        1 tttagaataa aaaaaaacat ttatatcaga atcaataaat tctatttttt tattactata
       61 tcgtcatcat tatcttgacc atttatgttt attgttccct gagtggggag tacttctttc
      121 aaattcaagt taaatttata ttccattact tctcctccaa tatataaaaa ttacttaact
      181 gtatccaaat ttaatatgta aataaatcat taatttttag ctaaattcta tgagaagttt
      241 atttttgttg tttttgctaa ttaactaaaa ctagtacaaa aaatttaaaa tagattatta
      301 aacctattgc ttattctatt cagtcatata tataagcgtc atcctttagt aactaacatc
      361 ccacactaca atagtattat aacatatcta ttcaacttca aattgttgaa atttaagcca
      421 tttatattta cttttcctca actttagaaa tgattttact aaccttatat ccgtaatacg
      481 cgtaacaaac acattcatta ataacaatca acactaagct atattagctt tttaaatgac
      541 tagggtaaat aaaataagga tcaaaaactt ttaagtttct gaccctttcc ttacttccga
      601 ttggttaata gtgatctttt gcagaataaa taaatatcgt ttcattttca actctatata
      661 tcagtctatg ttcatctgta attcttctgg accattttcc agataaatca tgctttaatg
      721 gctcaggttt tcctaatcca gcaaaggggg aacgatcgat atcttttatt aacttgttaa
      781 tcttttttat attgcttttg tttccttgct catgccaata aagataatca tcccaagcat
      841 catcagacca agccttaatc atcagattca acctcgatta agtcatgtgt tttaaatgca
      901 cctttggaga attgttcatc tcctcgacga attttttcca tgacgtaatt attagaaagt
      961 gttctcaacg tttcttgcat agaatcataa tctctttttg ataatacaac aactgtatct
     1021 tctacatctt tacttgttac aataagtgtt tcagcatcct cattaacttg tttcatataa
     1081 ctacgtaaat tttggcggaa atttgaataa gctactgctt ccattccttt caccccttat
     1141 ttatttctat aaaacaattg tacattatat tgtacaatta agcaaatgat taatattcta
     1201 acttcttata attaaaagtg ctatcacttt tagtttttag gataaaaagt gacgtcactt
     1261 tttgagtgct ggtacctcaa atcctgcttt ttcttgggca atcaataaat ctaacgcaat
     1321 acacataatc ctggattcac ttttgccgta cttctgcttt agttgctgca attctttttg
     1381 tctcttttca tccaacgtaa cgggtaaccg tttatttcct attgcaattg gcattagtac
     1441 tc
//
LOCUS       DACEMO010000310         1437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.318, whole genome shotgun sequence.
ACCESSION   DACEMO010000310 DACEMO010000000
VERSION     DACEMO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.318"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            177..374
                     /locus_tag="IW728_002722"
     CDS             177..374
                     /locus_tag="IW728_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026044.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            480..962
                     /locus_tag="IW728_002723"
     CDS             480..962
                     /locus_tag="IW728_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026045.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1020..>1437)
                     /locus_tag="IW728_002724"
     CDS             complement(1020..>1437)
                     /locus_tag="IW728_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1026046.1"
                     /translation="LWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTDVV
                     KGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKEWN
                     IPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          440 a          227 c          296 g          474 t
ORIGIN      
        1 tttttgaaaa aaattcttct aaatacaaca gctgttttaa ttgtgaaaac gaaaacagta
       61 cgctataatg cctgtaaggt tgatttttaa ctgtaaataa aaatcaatga gttaaatctc
      121 caaagaaata gcagtggtaa tgtggagaaa agttccagca gtttaggagg attttgatga
      181 cggaagaaat gattaattta ggagaacaat atgcatgcaa accaattggt tttacaaaaa
      241 cggtcattgg tgaagttgta tcaaaaatga caaactgcgc cgtggtcaaa gttgcccaat
      301 gtgctgcaga ggaccaagaa ttattagatg aaaaagcaag catggttgtt gcaaaatatg
      361 atacatttga ataaagaaaa caattggacg tgcggcgtgc tgcaagtcca atttctttga
      421 gaaggtaaat cagtattctt ttcctatttt ctggagaaga aaaagcgagg cgagttgtca
      481 tggtaacatt ctttacggtt ctattgtttt tattcgttgt tgtgatgttt ttatttcttt
      541 ggctggttcg aaaagaaatt atttatcgga ctgtgcgaaa tcgatgggtc tacttggtga
      601 tacccttctt agtgtcacta gtcatttggt atacactcat cagtcatcct acagcagatg
      661 aattggcaaa agggatcttg tcagcaatga tttttattag ttttttgttg gacagtagag
      721 gaatcacaga agaaggactt gttttgaata gctttgacaa aaaaggtgtt cctttttctg
      781 agatcaataa aattgtatta tatcagccaa aaggttctaa aatcgtaaaa atgaattttt
      841 tccgaaacgg atggcgagga ccgatgctga aattttctgc atcattagaa gaattagttc
      901 catttttgtc ccaacgctta aacgatgagg cagagattga tattatgatt gatccagaat
      961 aaaaacagag gttgcgacaa gatgtttgtc acaacctctg ttttttattt caaataaaat
     1021 tatccaaata cgcggaagcg atcggcatcg ttcatgtatt ctttttcccc agccggtgtt
     1081 tctggtgaac cgaatactaa ttgcgaacgt agtttccagt ttgaagggat attccattct
     1141 tttgcaactg cttcgtcaat aactgggttg taatgttgca agtttgcgcc caatccttta
     1201 tcaactaatg ctacccaagt attagctgtt gcgattccgt ttgattgttc tgaccaatct
     1261 gggaagttgt ctgcatataa ttcaaattgt tcttgtagac cttttaccac gtcagtatct
     1321 ttgaagaata atactgtacc ataaccattt ttgaaaccag ctaatttgtt ttgtgtattt
     1381 gggaaagctt ctgctggagt caatgggcga agtgcttctt cagtgatttc ccaaagt
//
LOCUS       DACEMO010000311         1425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.319, whole genome shotgun sequence.
ACCESSION   DACEMO010000311 DACEMO010000000
VERSION     DACEMO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.319"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..1392
                     /locus_tag="IW728_002725"
     CDS             100..1392
                     /locus_tag="IW728_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1026047.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          463 a          292 c          258 g          412 t
ORIGIN      
        1 tgacaccagc atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta
       61 gaattaattt ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc
      121 aaagaaacat tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa
      181 gaaaaataca aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa
      241 gaatgcatcc actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc
      301 gtccggttgt taatgaacga tgtttctgaa taccgtacct acctcgaatt gaaaaagcaa
      361 cgcttcaaat gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa
      421 cattgtttca tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc
      481 tcaatgacag agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg
      541 aagcgatttt atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt
      601 gatgaattca aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga
      661 caaacacagc gcttattgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat
      721 tttagtcatt tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac
      781 gctccttatg tatcgctcgt taaaaagctg tttccaaaag cacagctgat tattgaccgg
      841 tttcacatcg tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac
      901 caactattaa aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac
      961 tggaaacttc ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct
     1021 ggcttcaaag ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct
     1081 gctttgcgtg taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac
     1141 tacgtatcgt ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg
     1201 accttgagaa cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat
     1261 tcaaatggcc cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgcatat
     1321 ggctttcgta gcttttataa tttcaaattg cgcatcatga ttcgtcatgg caacgcattg
     1381 attttcaact aaaaaagatc aagaggaact gatccacttg atctc
//
LOCUS       DACEMO010000312         1411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.320, whole genome shotgun sequence.
ACCESSION   DACEMO010000312 DACEMO010000000
VERSION     DACEMO010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.320"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..390
                     /locus_tag="IW728_002726"
     CDS             <1..390
                     /locus_tag="IW728_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1026048.1"
                     /translation="FETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIELNQV
                     RKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVEDKE
                     MPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            374..1069
                     /locus_tag="IW728_002727"
     CDS             374..1069
                     /locus_tag="IW728_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1026049.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          528 a          206 c          284 g          393 t
ORIGIN      
        1 tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa agaaaaaata
       61 atgattttat ttggagatga agcaccggaa acattaacag attattgtta taagattgaa
      121 ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga taaacaaatg
      181 tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt aggacatatc
      241 acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta tgtagaagat
      301 aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga ggatcagaat
      361 gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta tttattcttg
      421 tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga aaaaagaagg
      481 agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac agattcctat
      541 ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc gagttatcat
      601 agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag gccttcaagc
      661 gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt acacagaatt
      721 acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt ataacaggat
      781 gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg agatcgaacg
      841 tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac aagtcactac
      901 agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag atcaagtatt
      961 agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg tgcaaacttt
     1021 ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa tcaatttctc
     1081 cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga ttcaatattc
     1141 attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt attccctaac
     1201 ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt cataaaaaca
     1261 aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag aataagagga
     1321 acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc gagtttgagt
     1381 ttatgatagt tgtttttgaa gcgatacggc a
//
LOCUS       DACEMO010000313         1398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.321, whole genome shotgun sequence.
ACCESSION   DACEMO010000313 DACEMO010000000
VERSION     DACEMO010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.321"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..588
                     /locus_tag="IW728_002728"
     CDS             <1..588
                     /locus_tag="IW728_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026050.1"
                     /translation="RGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYT
                     DGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVT
                     NGNTGSAKLYREGKTVSIYFVALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRS
                     TLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            751..1197
                     /locus_tag="IW728_002729"
     CDS             751..1197
                     /locus_tag="IW728_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026051.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1199..1336
                     /locus_tag="IW728_002730"
     CDS             1199..1336
                     /locus_tag="IW728_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1026052.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
BASE COUNT          501 a          228 c          302 g          367 t
ORIGIN      
        1 cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt atgtttggga tctagcacca
       61 gaatatgtcg aagataattt ggccacagat attaaaattt ctgaaattac tggtaaagca
      121 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      181 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      241 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      301 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      361 tattttgtgg ctttaaacgg aaaacgcagt ggtggaaatg attcaacgat actaacaatt
      421 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      481 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      541 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
      601 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
      661 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
      721 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
      781 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
      841 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
      901 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
      961 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1021 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1081 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1141 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1201 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1261 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1321 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1381 atttagggga atggggag
//
LOCUS       DACEMO010000314         1395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.322, whole genome shotgun sequence.
ACCESSION   DACEMO010000314 DACEMO010000000
VERSION     DACEMO010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.322"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            78..1247
                     /locus_tag="IW728_002731"
     CDS             78..1247
                     /locus_tag="IW728_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1026053.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          509 a          250 c          229 g          407 t
ORIGIN      
        1 aaataaatgt tatcaagata caagctattt tagctttatt ctttctgtaa aaaagaaagg
       61 agactaaata aactattatg aaaaaaggaa aacatgctaa aaaaagaaaa tattcatttt
      121 taatgatcgt tgtcgtgttg tttagcctta gtgcgttctc tacaggaact attcttggat
      181 ggcaatttta caataatgaa cactctcaga tagttgccca tgctcaatca gctcaaaaaa
      241 agttgatact gactaccaat gaacaacagc tcgttgaaaa aatcgatcaa ctattaagaa
      301 aaaatgatta tattggctca atttacgtaa agaaaaataa tcgcatcatc atggaaaaag
      361 gctatggcta tgcgaataaa ctcacgcaac aaccaaatga tccttctctt tattatcaga
      421 ttggttcggt tcaaaaagca ttgactactc ttttgatttt aaaacaagtc gagcgtgggc
      481 acctttcttt agatactaag ctggctcaat tctatccaca aattgctggc agccagcaaa
      541 ttactatccg tgacttgctg tatatgcgct caggcttgca ccgaactgct tctccaacca
      601 taccgatgac tgatgaagaa gtcgttcaat ttgcgattca gcacttaaaa tttacagatt
      661 atcagacctc tcattatgat tcattgaact ttaccatatt gactggaatt ttgatcaaat
      721 taacaaatca accgtatgaa aaattattga ccgatgatat catcgaacct ttaggattac
      781 agcatacaaa attttatgac caagtcaaaa attcatccaa tcatgcacta tcctatcaaa
      841 tgtctaaaga ggatgattac tttaaaccac tgacagaatc tgaaacagag atccataacg
      901 aattagggac tggtaatatc agtatgtccg tctatgattt aaatacgctg tttacacaag
      961 ttttatctgg aaaactagtt tccaaagact tactcttctc attatggcgt gaaaatgcga
     1021 atggacatcc atatagtggt ggggtatata gcggagataa ttatatcctg tcgcaaggaa
     1081 atatcgactc ttttgataca tctgctatat ttagcaaaga ttcacaggat gctatcatca
     1141 tggaaagcaa tataaaagct gacaaaacta tcaaactgcc tactgctgat ctaagaaacc
     1201 aaatctatga cctgatggaa ggaactacta agttggcaaa tagctgatat gatgaaaaga
     1261 tagcaaatac tacttctttg aatagcattt gcttaaaaaa agtactcgac taaacgtagg
     1321 aaagagtgtg ggacaaatct aaaaatcaat ttttgctaca gactttaatt ccaaataaac
     1381 agaacagaaa gaggt
//
LOCUS       DACEMO010000315         1389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.323, whole genome shotgun sequence.
ACCESSION   DACEMO010000315 DACEMO010000000
VERSION     DACEMO010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.323"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..415
                     /locus_tag="IW728_002732"
     CDS             <1..415
                     /locus_tag="IW728_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1026054.1"
                     /translation="GRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQRRLVYKSIV
                     EDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(580..>1389)
                     /locus_tag="IW728_002733"
     CDS             complement(580..>1389)
                     /locus_tag="IW728_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026055.1"
                     /translation="KDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELKTLPNS
                     VNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACEIGVKE
                     FYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRNKLVHS
                     PQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQITLSK
                     ELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          456 a          245 c          203 g          485 t
ORIGIN      
        1 tggaagaaat tatgacgaga tcatcgcttc agtcaattac ttgaagaaga aaaatgtgaa
       61 gctgatcatt actagtttac cgatcatggc tgaagcaatt ggcaatcctc tcctggataa
      121 attcattaag gatctgatca tccaaatttt ggccatgatc gctgaacaag aacggactga
      181 atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa gcaaacggcg tctacaaagg
      241 gcggcccaaa ttatacagtg cagacgcaaa agatccccaa cgtcgccttg tttacaaaag
      301 catcgttgaa gatcttaaaa atggtgttgc gattgctaag attgctaaag actacaatgt
      361 tacgagacaa accgtgtata gaataaaaaa agacagcatg gtcaatgaca aatagttgat
      421 catgctgctt ttattatctt ctcatcttta atttcctaca tataattact atctataaaa
      481 ttaagtctta ctcagaacag ataaagcata aaattccaac aaaccatttt ttacttaact
      541 aaataaagag tctgttaata taatcttcac ttatttacgt taatccccta ctgtatttgt
      601 tggttcctta tctatatttt ctacaaaatc cttattatct tgtgatttag cttcgtataa
      661 ttctttgtat gtcgacacat tggttatact aattcctata gctagttctt gagactctac
      721 ttttctctct agctcctttg acaaagtgat ttgagattta tttggttccg ttggagtcaa
      781 ttctatagat tcttcataaa aatctctata cataaagcta tcattaattt tataaaaaaa
      841 attaaatgtt tttagcactg ccatataaca ttcaaaacat tcttctatag ttggtttact
      901 tccttgagga gaatgaacca atttattacg tttttctatg aaatttttaa tttcctttct
      961 tatttctttc ggaaactcaa tattaaagta ccctttaaac atgctcccta ataatttgtc
     1021 tacaggggga gattgtaaat tctcaaatat tagagttaaa tcaggtttct cttgttgata
     1081 aaattccttt acccctattt cacaggctgt aattatcgaa ataaacctat ctctagtatt
     1141 tgatatattt ttacttcttt taaggagtat aagagaaaag gggatacttt cgtgtccatg
     1201 catgtaatta actaattgtt gagcttcaga agttatatct ctaattatat agttaacgga
     1261 attaggaagt gtctttaact ctaatgaaat ttctatccaa ttactatctt ctaatctgta
     1321 tatgctcgaa gtataatttg attcgtcagc aagcatcgct acatcccaaa cactgatcat
     1381 aaaatcttt
//
LOCUS       DACEMO010000316         1385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.324, whole genome shotgun sequence.
ACCESSION   DACEMO010000316 DACEMO010000000
VERSION     DACEMO010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.324"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..132
                     /locus_tag="IW728_002734"
     CDS             <1..132
                     /locus_tag="IW728_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1026056.1"
                     /translation="STSPSTATFEQRNTATPNNNGLNASQNPFGGQSIDISDDDLPF"
     gene            177..410
                     /locus_tag="IW728_002735"
     CDS             177..410
                     /locus_tag="IW728_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026057.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(569..691)
                     /locus_tag="IW728_002736"
     CDS             complement(569..691)
                     /locus_tag="IW728_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1026058.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            881..1105
                     /locus_tag="IW728_002737"
     CDS             881..1105
                     /locus_tag="IW728_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026059.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
BASE COUNT          454 a          266 c          264 g          401 t
ORIGIN      
        1 tccaccagtc caagcaccgc cacctttgaa caacggaata cagcgacgcc taacaacaat
       61 ggattgaacg cttcacagaa tccatttggt ggacaatcaa tcgatattag cgatgatgat
      121 ctaccgtttt agtttgagaa agaaggagta agcatggcaa aaaaattagt ctgacgatgg
      181 atctattgat acccgtaatt gcatttatag acgggatttc tttgggcgtc actgaaaatc
      241 ctttggtaaa tagaattgcg aaaggatcac tatttcttgt attcgtgtat cttgtctgtt
      301 cttatctttt aaaaagagca ggaaaggata attccttttg gcgattgact aaagtcacag
      361 tagcagcgac agctgttata cttacggcta ttctaccgct ttatatgtag agttaacggg
      421 aaatcgttga acttcttgcg aaatattgct ataatgaaca tgaaaaagag gagttgcgct
      481 aacaacccct cattaacagc accgattaaa gaacggtggc agaatattag ttatttggaa
      541 ataaccgctg acctgctaaa gtagacggtt atttcttttt gtcattttta agcatattta
      601 caatcaaact aatcaaagca atggtaaaca taccaaaacc aagaatggtt tgaattgtct
      661 catatgcgga caaaaggctt actcctttca tagattttag cacttacatt cataagcacc
      721 accacctttc cagaagtcgc caccgtcttc aaactttact gtcttgacta ttatagcata
      781 tctaaaatcg aataaaaatt ctcaatttta gctgtttttg ctataatttt agcgaggtga
      841 tcgtgatgga acaatatatt ggtttactcg ataaattagg gtgatcaaga cacagccatt
      901 gcttgtgcga tttacattac gcacgagtca tgaaccaatc aattgtgtag tggccaatat
      961 agaaatagcg gataaattgc tgattttgga agaaggcaag tatactattt cagtgacagg
     1021 tcacttcaat aaacgaaagc aactagtcat tgaatcaatg caaattagaa accctgatca
     1081 ttttacgcga tcgatgagca tataaaagaa gagctgcagt cctaaatgag gattgtagct
     1141 ctttttgttt tacatagaaa cagtaaaaat gaaaaggcct gtttaggaag gcacaagcgc
     1201 cttctcaatt gaaagacctt tctctcactc caaccgccac cgccgttcga gaagaaagga
     1261 agtgaacgct acgcgttcac taagataaag ggtcttgcat agcaagacaa aatagaaaaa
     1321 atttcctctc accaaaaggt tcgctcctgt tcgttatctc tttattgatt tgtcagaccg
     1381 gcaac
//
LOCUS       DACEMO010000317         1376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.325, whole genome shotgun sequence.
ACCESSION   DACEMO010000317 DACEMO010000000
VERSION     DACEMO010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.325"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>1376)
                     /locus_tag="IW728_002738"
     CDS             complement(join(<1..808,808..>1376))
                     /locus_tag="IW728_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HAQ1026060.1"
                     /translation="KYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINAYQ
                     EFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQFR
                     EGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENAFL
                     KELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRKNP
                     DQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRKSR
                     RYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPF
                     MDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNK
                     LKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNN
                     ERIKQKLN"
BASE COUNT          348 a          284 c          248 g          496 t
ORIGIN      
        1 cagtttaatt tctgttttat ccgttcatta ttgtagtaat agatatagct atcgatggct
       61 gattttaatt cctcgaaaca gttataaact ttcccgtgaa aaatctcttg ttttaataag
      121 ccaaaaaaat tctccataag tgaattatct aagcagttcc ctttccgaga catgctttga
      181 aagattttgt actccttcaa tttgtttcta taagctttca tttgatacgc ccaaccttga
      241 tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc ttcctccaaa
      301 ccttccatta ccgctaatgc attgggcttt tcagagatac gataggataa aatctcacta
      361 ttgaacatat ccataaaagg atctagatac aatttctttt gacggataat gcctttggca
      421 tccacttcat ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg gcagatactg
      481 gtgtagaaac gacgatggat ccgatttttg gctactgctc cgacttgtcc tcgataagaa
      541 ttgtagcgcc gtgatttacg tgtgtatgtc gttactttta ttcctagctt cttgatcagg
      601 cgctgaactt tctttttatt gatagagaaa ccgcggtttc ttagttcatt ggtcatccgt
      661 aaacagccat aatccttgtg cttctcccga actttaagca tttccgcttc aatctcttga
      721 tctggatttt tacggtccaa tcgtttttgc cagtacataa agattgcctt agggaacttt
      781 aaagtttcta gaagctcaac taatttaaac ggtcctcgga ggcttgcgat gattcgtgcg
      841 attgcttcat tcgtcgttgt tgggcttcct gttttcgcag cttcctcaat tcttttaaaa
      901 aggcattttc aatttgaagg gaacggactc gtttctccaa ctcctcgatg cgttcacgtt
      961 ctttagacgt tgctttagtt gtagattttt tcttattttt gggttcattt ttcttagaca
     1021 agataggtgg acatcctttc gtttttgaga gtccatcgat cccaccctca cggaattgac
     1081 gcatccaact agcgatcaaa ttagggttgt tcatgttaag tgtgttggca acttcacgat
     1141 aggacagctc acttgttaga tataactcta tcgcatccag cttaaattga acagaatatt
     1201 ttttcttttg tcgctttctt actagaccct cttcaccaaa ttcttgatag gcattgatcc
     1261 agcgcttcac ttgtgatgga gccttaatgg aatatttctt tgccagataa tcacttccgc
     1321 ctttaccatc caagtaatca tgaacgatct ttaatttaag ttcaaaagta tactta
//
LOCUS       DACEMO010000318         1374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.326, whole genome shotgun sequence.
ACCESSION   DACEMO010000318 DACEMO010000000
VERSION     DACEMO010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.326"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            33..1372
                     /locus_tag="IW728_002739"
     CDS             join(33..474,474..1372)
                     /locus_tag="IW728_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1026061.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DACEMO010000319         1371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.327, whole genome shotgun sequence.
ACCESSION   DACEMO010000319 DACEMO010000000
VERSION     DACEMO010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.327"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            276..>1371
                     /locus_tag="IW728_002740"
     CDS             join(276..552,552..>1371)
                     /locus_tag="IW728_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1026062.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFR"
BASE COUNT          482 a          262 c          274 g          353 t
ORIGIN      
        1 acaaaatatg gtcgaaaggt gtacgaatca cataattatt tacatgaaga gctttatagc
       61 tcaataaatg aagcgttaga atcattctca ccagaccaaa tttcaaccat taaacagatt
      121 ctagaaaaag tatcttatac aatcaatgaa tatcaagtaa gtctgacaag aaaggaagat
      181 tgacatgaaa aaaatgattt ttgaatcggc tacactagac tagacagaaa aaataaggtg
      241 tgtaaaataa atgaaaacac actggaggaa tttttatgtc aagacgtcaa cgtagaacct
      301 attcaaaaga attcaaacaa caagtcgtcg atctttatct cgcaggaaag ccccgagctg
      361 aaatcattcg agattatgag ctgactacct cggcctttga taagtgggtc aagcaagcgc
      421 aaactactgg ctcgtttcag gaaaaagata acttgacccc tgaacaagta gaactaattg
      481 cattacgaaa gaaaaataaa cagcttgaaa tggagaatga cattttaaag caagcggcgc
      541 tgatattggg acgaaaagac aagtaattga taccaataag cacaaatact caatatcagc
      601 gatgtgtaaa gtgttaaaca tctcttgcca aacctattat tatcaagcca aaccagttga
      661 agatgaatca gaactggaag aactcgttca agaagaattt attcgcaatc gaaaagctta
      721 tggtaccagg aagctgaagc agtgcttggc caatctaggt cttcaagtga gtcaacgcag
      781 aatcggccgt atgatgaaac agcgtggctt gcgttcaaca tacacattgg ctcatttcaa
      841 attccattct tctacttgta acgaagccaa gaccgccaat attctagacg gaaccttcct
      901 acaggaaaag tcattagaag cgattgtaac cgaccttacc tatgttcgtg ttgggaagaa
      961 atggcagtat atctgcctga ttcttgattt gtttaatcga gaaatcatcg gctattcatg
     1021 cggggagaag aaagatgcga cgttagtcaa ggaagctttt gcaaagattc cgtactcgtt
     1081 gaataatgta caactttttc acacggatcg tggaaaagaa ttcgataacc agacgattga
     1141 cgaaatttta tgggccttta atatcacgcg ttccttaagc aaaaaaggct gcccctatga
     1201 taacgccgtt gcagaatcca cctataaatc tgtcaaagta gaattcgttc atcaataccg
     1261 ctttgagaca ttggctaatt tgcgattaga gctatttgat tacgtccatt ggtggaatac
     1321 catccggctg cacggcacgt taggctatga gacaccgatc agttttcgac a
//
LOCUS       DACEMO010000320         1369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.328, whole genome shotgun sequence.
ACCESSION   DACEMO010000320 DACEMO010000000
VERSION     DACEMO010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.328"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1369
                     /locus_tag="IW728_002741"
     CDS             <1..>1369
                     /locus_tag="IW728_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1026063.1"
                     /translation="EKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGT
                     DLEAKIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLV
                     LAAVLLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADM
                     IHQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADD
                     IKLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSAT
                     RDQYKTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQ
                     AKQAAQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAW
                     TWAPGVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESA
                     LPFYIV"
BASE COUNT          576 a          255 c          275 g          263 t
ORIGIN      
        1 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
       61 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      121 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
      181 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
      241 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
      301 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
      361 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
      421 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
      481 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
      541 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
      601 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
      661 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
      721 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
      781 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
      841 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
      901 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
      961 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1021 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1081 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1141 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     1201 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     1261 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     1321 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgta
//
LOCUS       DACEMO010000321         1358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.329, whole genome shotgun sequence.
ACCESSION   DACEMO010000321 DACEMO010000000
VERSION     DACEMO010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.329"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1034
                     /gene="fss3"
                     /locus_tag="IW728_002742"
     CDS             <1..1034
                     /gene="fss3"
                     /locus_tag="IW728_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1026064.1"
                     /translation="AFSVSLNRLMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVD
                     KLSYNLHEIKEDWYVVAQAIDVDATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKI
                     QHKLTAEQVLNKTIVLFNYVYENKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQ
                     TKAHLENGSQTFTHGDVVDMFDDVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSG
                     KIDYEVNDKEFTKTVLAKKVDTGKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQT
                     LTPKEVPTILSTPKQPETPTVPSGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATA
                     GYYFWNRRN"
     gene            1147..>1358
                     /locus_tag="IW728_002743"
     CDS             1147..>1358
                     /locus_tag="IW728_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1026065.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQP"
BASE COUNT          482 a          236 c          299 g          341 t
ORIGIN      
        1 aagcattttc tgttagtttg aaccgtttaa tgctctatga tttgcctgag gaagaagata
       61 gtttaacttc tcttgcgact tggaaagacg gaaacaaaga attaacttct ctggattcta
      121 ccgagctagt tgataaattg agttacaatc tacatgaaat caaagaagac tggtatgtgg
      181 tagcgcaagc cattgatgtg gatgcgacaa aagctgccca agaaaaagat gaaaaagcca
      241 aaccagtggt gattgcagaa acaagcgcaa cgttggcgaa caaagagaaa acaggaactt
      301 ggaaaattca gcataaatta accgccgaac aagttttaaa taaaactatt gtcttattca
      361 actatgtgta tgaaaataag gaagcttttg aagcaggcga taagccagta gcgaaggatg
      421 ttagcttgaa taaccaagcc caaactgtca gttgtacagt ggaacaccat gtttctatcc
      481 aaacaaaggc ccacctagaa aacggttctc agacctttac tcatggtgat gtggtggata
      541 tgtttgatga tgtgtcgatt actcatgatg tactggatgg ctcaaaagaa gctttcgaaa
      601 caattctgta tgctttatta ccggatggta caaacaaaga aatttggaaa tctggcaaga
      661 ttgattatga agtaaatgat aaagaattca ccaaaactgt gcttgcgaaa aaagtagata
      721 ccggaaaata tccagaagga accaagttta cttttgcgga aatcaattac gataaagatg
      781 ggaatatcaa tggaaaacac aatgaagatt tgaaagaaaa atctcaaacc ttaacaccaa
      841 aagaagttcc aaccatacta agtacgccga aacaaccgga aacaccaact gttccaagtg
      901 gctctcaaga atctagtccc acagtgaaga ccttccctca aaccggggag aaaaattcca
      961 atgttttact gttcattggt tttactttga tttttgcgac ggcgggctat tatttctgga
     1021 atcgccggaa ctaaggtgat gagatgatac aagtaaatcc taagaaccaa cgtgcgcgac
     1081 cgcctcctaa aaaagtagtc gcgctatttt tgtatccaaa actaacaatg aaaagaggaa
     1141 aagaagatgg aattgaaatt tgtcgtgcca gatatggctg aaacctttgg aaagttaagt
     1201 tatgccggtg aaggagaaat tataacagaa ggatatggac ggaatactac ggtgattggt
     1261 cgcagttatc atttgtattc cagtaagcaa cgggccgatg atattgaggt ggtagtagct
     1321 gcggaagccg gtgaaaagga ttttgatcaa gaccaacc
//
LOCUS       DACEMO010000322         1337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.330, whole genome shotgun sequence.
ACCESSION   DACEMO010000322 DACEMO010000000
VERSION     DACEMO010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.330"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1337
                     /locus_tag="IW728_002744"
     rRNA            <1..>1337
                     /locus_tag="IW728_002744"
                     /product="23S ribosomal RNA"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DACEMO010000323         1332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.331, whole genome shotgun sequence.
ACCESSION   DACEMO010000323 DACEMO010000000
VERSION     DACEMO010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.331"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            69..770
                     /locus_tag="IW728_002745"
     CDS             69..770
                     /locus_tag="IW728_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1026066.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          469 a          242 c          237 g          384 t
ORIGIN      
        1 taaaacttta caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg
       61 actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg
      121 attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt
      181 cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct
      241 tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg
      301 actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca
      361 cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg
      421 aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag
      481 tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg
      541 taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata
      601 tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg
      661 aaactgctgg acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa
      721 agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa
      781 aataagcagt gaccctgata aatatcaggg tcactgctta ttttcctccc taaaacttct
      841 gaaagatccg gcagttttag atcactggac aagactcaaa gattttctat gtgtgtttca
      901 gttatgtaat attcactgtt tgcaaaaaat actgttagat taactagatg aagcgtcttg
      961 tcctcatccg atctagatta ttcgatattc ctcgacaata aacgattact cagtgagggc
     1021 aatcagagga acggataatg ataaataagc tgtaaaaaat agcaaaataa caataatgat
     1081 taaaagtatg taaaaaatag ggctttgaca aaaacgtgtc acagccctat ttcgattaaa
     1141 cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg ttcataaaca tgaggagctg
     1201 ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta
     1261 aacggcgttc aaaagcttgc atacgcggta ataagaacag ccaggtcaaa aacatgagaa
     1321 gccgtttttc ct
//
LOCUS       DACEMO010000324         1319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.333, whole genome shotgun sequence.
ACCESSION   DACEMO010000324 DACEMO010000000
VERSION     DACEMO010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.333"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1285
                     /locus_tag="IW728_002746"
     CDS             <1..1285
                     /locus_tag="IW728_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1026067.1"
                     /translation="IKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEIRRCPLC
                     KQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTYIVDRNC
                     FIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWLPRCLLM
                     DEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKVKVIVTD
                     MNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQAKYYRQL
                     KALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFYYQEILQ
                     AFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKIENLHTH
                     IKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          429 a          261 c          245 g          384 t
ORIGIN      
        1 tatcaaacta ctacttggaa tcaaagataa aaacattttc atcacaaatg tggaatcgaa
       61 gagcattaag aatatcaaga gtcttgttgt ttctgcgacg ttaagtaaag aaattcgtcg
      121 ctgtcctttg tgtaaacaga tgaatcatga aggcatgatc gttaagaacg gaaagaaaaa
      181 atcgctcatt caacttaata aatgtgcgaa ccaactgact tatcttgctt tagcgaaaca
      241 acgctaccac tgtcgtggct gccacaccta ttttacggcg aatacctaca ttgtcgatag
      301 aaactgcttt atcgctaaac aagtgcgcta taaaatttta gaagagctaa ctgaaaaaca
      361 agcaatgacg acaattgcta agcattgcgg cgtttcttgg tcgaccgttt ctcggacact
      421 cgcttctctg atccccatga caaaagtaaa aagaaattgg ttaccgcgtt gtttattaat
      481 ggatgaattt cgttcactga aaaatcaagt cggtccctac tccttttctt gtatggatgg
      541 cgatactgga aaattactgg atattcttcc ttcacggaag aaaaaggctc tcgtttctta
      601 ttttatgcaa tttgaacgac gcgcacgatt gaaggtaaag gtcatcgtga cggacatgaa
      661 tgtttcttat gcctctctga taaaggaatg ttttccaaag gcaaaactcg ttgtggatcg
      721 ttttcacatt gtcaaacatc tcattcgtaa atttgaggat attcgcgtac gaatcatgaa
      781 aagctttgat cgaaacgatc caacacaagc caaatattat cgacaattaa aggcgttgtc
      841 tcggcttctg atcaaacgac aggacaagct tgtttatgac aaatggacca aatggcgcaa
      901 ttttggttgg gcgtatctca ccgaaagtga agtggtggat cgtctgcttt cgatctctga
      961 cgaattacga atcgcctatt tttactatca agaaattctt caagcgtttc acgacaaaga
     1021 ggcggacact ttctttaaac ttgtaagaac gatgcccaat tccgttccaa aagaactgca
     1081 ttctataaaa aaagctttta taaactatga atccggcatt cgtttagccc tcgaattgcc
     1141 ttattcaaat gccaaaatag aaaatctgca tacccacatc aaagcattga aacgagtcgc
     1201 ttatggcttt cgaagctttc ggaaaatgaa gaccagaatt ttcttactaa ataatctgat
     1261 tacctatgaa tcaaaaaaca tctaaagtag aaaaggaatt ctaccttaga tgttcttat
//
LOCUS       DACEMO010000325         1311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.334, whole genome shotgun sequence.
ACCESSION   DACEMO010000325 DACEMO010000000
VERSION     DACEMO010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.334"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..505
                     /locus_tag="IW728_002747"
     CDS             131..505
                     /locus_tag="IW728_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1026068.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IW728_002748"
     CDS             complement(619..1248)
                     /locus_tag="IW728_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1026069.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
BASE COUNT          444 a          185 c          229 g          453 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc a
//
LOCUS       DACEMO010000326         1310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.335, whole genome shotgun sequence.
ACCESSION   DACEMO010000326 DACEMO010000000
VERSION     DACEMO010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.335"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            252..925
                     /locus_tag="IW728_002749"
                     /pseudo
     CDS             252..925
                     /locus_tag="IW728_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1158..>1310
                     /locus_tag="IW728_002750"
     CDS             1158..>1310
                     /locus_tag="IW728_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ1026070.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMH"
BASE COUNT          454 a          168 c          282 g          406 t
ORIGIN      
        1 ggatattcca tgatgtttgg ggaggtcgaa aaaaacttct ttatgaagta catcaaaggt
       61 cgaggttatg tggatacagg tggcagtgtg attagtgagt tttatacgcc acttgtacca
      121 aaagggtatg atttcttaga ttcaattagt aaattagaaa agatgaatga ttgagtgatc
      181 ttcaatactg actttatact tcaagtaacg tataatactt ttatatagta ggaagaaagg
      241 ggataattaa tatggaaagt aatgattctt atttagagta tgcaaaagca aataaggata
      301 agtttatttc ttatattatc aaagggaaat cccccgaagt tggacgagac gcagttttta
      361 tggcagggag tcctggggct ggaaaatcag aggttgccat tggattagct gagaattatg
      421 ataaccatgt gattattgat gcggattact ttcgcaccca gtttcctgat tataatggaa
      481 aaaactctag tctctttcaa aaagcatctt cttggttggt agagcaggca tttagatatg
      541 tcttagatca tggctattct tttattctgg atgcaacttt tgcaatatta agtgctgaaa
      601 aaaatatctc gcgggcgttg aaaaaacatt ttcgagtgac gattttctat gtttatcaag
      661 accctaaaat cgcttggaat ttacacaaga acgtgaaatt gcagagggac gtcatgtgcc
      721 gaaaaaggca tttattaacg cttttttcaa atcaagagag aacattgcaa aagtaaagag
      781 taaacatcca gatgtattgc ttcatatcat aataaaagat tatacgaata atatttccga
      841 agttcattat gctacggata atctagaatt atttttacca ctagggtata gtaggaaaga
      901 tttggaggtt caattacatg actgagaaag aacaagtaac gaaaattgta aagaaataca
      961 ataagagcat tgcggattta tcggaaaatg ctactgcaaa agagttcaaa acggtgatta
     1021 aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt agttggatta aataaaaaat
     1081 agtaggacaa attaataatg tatgagccaa gagcaaaatg ttcttggctt ttttctttgc
     1141 aatgaatgag gtaatgtatg agatttttag atttattcgc cggtattggt ggctttcgtt
     1201 taggtatgga acaggcgagc catcattgta tcggtttttg tgaaattgat aaatttgcta
     1261 gacgtagtta taaagcaatt catgatacaa gtaaggaggt ggaaatgcat
//
LOCUS       DACEMO010000327         1284 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.336, whole genome shotgun sequence.
ACCESSION   DACEMO010000327 DACEMO010000000
VERSION     DACEMO010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.336"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            117..362
                     /locus_tag="IW728_002751"
     CDS             117..362
                     /locus_tag="IW728_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1026071.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            459..803
                     /locus_tag="IW728_002752"
     CDS             459..803
                     /locus_tag="IW728_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026072.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
BASE COUNT          375 a          242 c          226 g          441 t
ORIGIN      
        1 tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta ctattgattg
       61 catgtgtagc tttctatagt cattataact attctactat aatgaaaggt gattaaatga
      121 gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc ttaagaactg
      181 gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta ttcaaatatc
      241 gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat atcgctgact
      301 tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc aaactgaagt
      361 gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa caatctatat
      421 ataaaggaga catttctatg tgattatggc atgaaaaatt gatttcaaag cttcctcgtc
      481 aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc tggggaaaga
      541 aacatgctac tgtggattat gtttttcact attctcctta tagattattc caatatcatc
      601 agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag tggaaaaatc
      661 ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta gagcgcaacg
      721 tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca aatttggagc
      781 aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct cttacttcat
      841 taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg gttatcttca
      901 tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac ttccctagac
      961 ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc tgtatacgat
     1021 gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct tcttctacac
     1081 cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc catgcatcgt
     1141 cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc tgtcgtggct
     1201 tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc tgccattgct
     1261 agcgttaata ttgatagtaa aaat
//
LOCUS       DACEMO010000328         1271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.337, whole genome shotgun sequence.
ACCESSION   DACEMO010000328 DACEMO010000000
VERSION     DACEMO010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.337"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            96..1181
                     /gene="xerS"
                     /locus_tag="IW728_002753"
     CDS             96..1181
                     /gene="xerS"
                     /locus_tag="IW728_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1026073.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          508 a          214 c          193 g          356 t
ORIGIN      
        1 atttcagagt ataccaattg acaaaacact ctataattgg tatagtccaa ttatagagat
       61 actcctctcc cctacttatc gaaaggatga ataacatgaa aaatacagaa atatatcaaa
      121 aaatagatac aatcatactt catatgcccg attatataaa agattttgtt caagatcgtg
      181 aggataaaga tcaatcacct cgaactactt tagaatatct aaaaaattat aagctctttt
      241 atgaatggct tctgagtgag tctatcgttc ccgatacttc aatcacctcg ataagacagt
      301 taactgccta tgatctaaac ttgtacaaat ctcatttgaa acgtcgcaca aaggaaaatg
      361 taaaaaaaga gacaacaaaa actaagctca aagataataa taacttaggt ctatctactt
      421 ctactatcaa tagaaatatc acagcattaa aggttctatt taaatatctt tcgaaaagtt
      481 ccaataatcc tttaggaaaa ccatacttag aaaataatcc tatggatcaa gtagcaacta
      541 tcacagataa aactacgtta gctgcccgtg caaacgcaat cgagaaaaaa ttatttctag
      601 acgaagatac acaaaattat ctagattata ttgccaatga atataaaaaa actttgagca
      661 aacgagcttt gatctattat caacgagatg tagaaagaga cctggcgatc aatgcattga
      721 ttttaggatc tggattaaga ctctccgagg ttgtaaatat taatttagag gacctatctt
      781 tagaaaagaa taatgtggta gtaactagaa aaggaaataa gagagacgca gtaaatatag
      841 cagcatttgc aatggaatat ttagctaatt atctagctat tcggaaagaa cgatacaaag
      901 taacagaaaa cgaaaaagct ttatttctag caatttatca aggagaagcc aagcgtatat
      961 ccggtattgc aatcgaacgg atggtagcaa aatattcaaa aggattcaga gtccaagtca
     1021 gtccacataa attacgtcat actttggcta ctagactcta tcaacagaca aattcattgg
     1081 tattaactgc ccaacaattg ggacatacaa gtacaaatac aacaacttta tatactcata
     1141 tagacaatgc agctacaatt gatgctttga acgctttgta aatgtagtaa aaaatagtat
     1201 aataatgaag cctgtctcta aaattagata aaagcccaac tttaaaggca agcttcatta
     1261 tcatttttat a
//
LOCUS       DACEMO010000329         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.339, whole genome shotgun sequence.
ACCESSION   DACEMO010000329 DACEMO010000000
VERSION     DACEMO010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.339"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..862
                     /locus_tag="IW728_002754"
                     /pseudo
     CDS             1..862
                     /locus_tag="IW728_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          444 a          221 c          209 g          372 t
ORIGIN      
        1 cctcttccga aaaggtatta ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg
       61 gcatcactac acgccccaaa ccatgtccaa catgactaaa gtgctgactg aagaagtaaa
      121 tgcatttaaa gttagaactc taaatgataa gtatgtcgct attttcatgg atgctactta
      181 tattccttta aaacggcaaa ccgtctccaa agaagcaatc tatattgcca ttggtatacg
      241 agaagacggc actaaagaag tactaagtta tgcgattgct ccgactgaat cgacctacgt
      301 ttggaatgag ttgttacagg acatttactc cagaggggtt catgatgtct tactgtttat
      361 taccgatggc ttaaaaggca tgaaagatac gattcaccag atttatccta aagctaaata
      421 ccaacattgc tgtgttcacg tttcccgtaa tattgctcat aaagtacgtg tcaaagatcg
      481 aaaagaaatc tgtgatgatt ttaaggctgt ttatcaagct agctcaaagg aagaggcaaa
      541 taccttttta gggagtatga ttgagaagta gcaaaaaact tatcctaaag tgacgcagtc
      601 actgataaaa aatcaagatt tattgacttt ttatgagttt ccgcctggta tccgccgaag
      661 catttactca actaatttaa tcgaatcttt caagaaacaa atcaagaaat acagccgcag
      721 aaaagagcag tttcaaaatg aagaatcact agagcgtttc ctagtatcca tctttgatac
      781 atacaatcaa aaattcttaa atagaagcca taaaggcttc caacaagtga cggatacatt
      841 agcttcaatg tttactgagt aacccattat tttgcagaag gacgagttat ttacacaaaa
      901 ttattgacgc tccctccaaa ttacttcatc aaaacattga tataattttt attatatcag
      961 tatttattct tgatacataa atgagaaaat aaatacttta aatctattga ctacaagtag
     1021 tcattaagtc ttcataagat gtacaatatg acattaaaaa aataaaagct tacgaccatc
     1081 cgttaaatct gacactaaat aatggactgc tttcttttgt attagcaggt tacgttgact
     1141 atgaatattc accagattat ttggagaaac ctcttcaaca cgagaacaat gatattaatt
     1201 ttagatttgt ctacgagtca aaaagtaaaa catggttagt tgatca
//
LOCUS       DACEMO010000330         1244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.340, whole genome shotgun sequence.
ACCESSION   DACEMO010000330 DACEMO010000000
VERSION     DACEMO010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.340"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..453
                     /locus_tag="IW728_002755"
     CDS             1..453
                     /locus_tag="IW728_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1026074.1"
                     /translation="MDMSMKLIHQAERYLAEKAYRTQKKEFLPKTAVTNRKENKKERQ
                     LFAKGDRIFVNEYQKEALVYEDIGEDTIDVYLDKKIIHVPRQRVRLVRSAEDLYPTGY
                     DLDSLFIDYKTRKRQRDLERGSKKAHKVLVKEMRKRQEERRVNDENSK"
     gene            579..1133
                     /locus_tag="IW728_002756"
     CDS             579..1133
                     /locus_tag="IW728_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1026075.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT          408 a          199 c          276 g          361 t
ORIGIN      
        1 atggatatgt cgatgaaatt gatccatcaa gcagagcgtt acttggcgga aaaagcctat
       61 cggacacaga aaaaagaatt tcttcctaag acagcagtta caaataggaa ggagaataaa
      121 aaagaacgtc agctctttgc aaaaggtgat cggatatttg tcaatgaata ccaaaaagaa
      181 gcgttagttt atgaagatat aggtgaagat acgattgatg tatatttaga caaaaaaata
      241 atccacgtgc ctcgtcagcg tgttcgtctg gtgcgttcag cagaagatct gtacccgaca
      301 ggttacgatt tagacagtct gtttatcgat tataaaacac gtaaacggca gcgtgaccta
      361 gagcgaggat caaaaaaagc tcataaagta ctagtaaaag aaatgcgcaa acgacaggaa
      421 gaaagaagag taaacgatga aaacagcaag tgattatctc aaattagctc ataatatcag
      481 tgtctgtaaa gaaaaataaa aacagtgaaa tcattggttt tattccccgt gtaagctaaa
      541 ataaaaagaa aagagtgctg aggaggtagg gcgataaaat gaaattaagc acagactggc
      601 gtaaagaaat acaaacgatt ccgaatcttt tatcgatatt ccgtattttt ttgctgccga
      661 tttatcttta ttttgtgctg agacagtcat tttatatagc aggagcggtc attgttgtct
      721 caggtttgtc tgattatcta gacggggtca ttgctcggag atataatcag gtaacagact
      781 tagggaaggt gcttgatccg tttgcagata aattgacaca actttttctg attttgtcta
      841 tggcatggta tcgaccgtgg ctttggctgt tgtttggtct attcttgata aaagaaggct
      901 ttatgtttgt agctggatta atcgggctat ctaaaaatat caaattaagt ggagcgaagt
      961 ggtatggcaa agtagcgact gctgtcatct atgttggcat gatccttttg ctgcttttcc
     1021 cagaattacc aacgctgtgg gtgagagtca tttttgccgt catcacttac ggtcttttac
     1081 aatcttttgt cttgtatgct gtcgagtacc gaaagatgtt tcaaagaaaa taaaaaaggg
     1141 gtgtgaccta tgtcgcattc ctttttttga taaatagact cccagtagct acttctcgat
     1201 aataagccaa ccgaaccaaa aacaggagaa gttgtttttg aaat
//
LOCUS       DACEMO010000331         1236 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.341, whole genome shotgun sequence.
ACCESSION   DACEMO010000331 DACEMO010000000
VERSION     DACEMO010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.341"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            242..1153
                     /locus_tag="IW728_002757"
     CDS             242..1153
                     /locus_tag="IW728_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1026076.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          486 a          187 c          241 g          322 t
ORIGIN      
        1 attagttacg ttaaatacgg aacgcaagat agacttgaaa aacggtaaca aaagaagtag
       61 tatattaagg tgtgatgtgc tacttgacat ttattctgct gagcgaactt tctggattgt
      121 gtagccttat atctattcag gacgtatcga gcagattagt aaaggtgttc ttgatgattt
      181 tcatcttgaa cacctttgtt tttagcaatt cgcaaaaaac gaaaagtagg agaattgata
      241 aatgaaaaaa agatttttag cacttgccat cgtactaggc actggcttat tgtcaggctg
      301 tacgaatgcc ggtgaaaaaa cagccgttag ttataaaggc ggtacaatca gcgaacaaga
      361 agtaatggat agcttgaaaa aaatgcaagg agcagattcc gctgtgcaac agttgatcgt
      421 ttatcaagta tttgaagata aatatggaga tgacgtatca accaaagaaa tcgattctca
      481 atatgatcaa acaaagaaac aattgggtga ttcatttgat tcacaattga aatctgcagg
      541 ttacacagaa caaacattca aagacagtat caaacaaagt ctagcctttc aagaaggtct
      601 aaagaaacat atcaaattga ccgatgaaga tttaaaaacg gcttgggaat ctttccatcc
      661 agaagtggaa gctcaaatta tccaagtagc aagtgaagat gatgcaaaag atgtgaagaa
      721 agctgctgat aagggagacg atttctctaa attagccaaa gacaagtcaa ctgatactac
      781 gacaaaagaa gatggcggaa aagtgaaatt tgattccact actacaacgg ttccggcaga
      841 agtcaaagaa gcagccttta agttgaaaga tggtcaagtt tctgatgtga tcacttctac
      901 caatgcttca acttatacta cggaatatta tgtggttaaa atggtgaaaa accaaaacaa
      961 aggcaacgac atggataaat acaaaaaaga attgaaagaa attgcaacag acacaaaatt
     1021 aagtgacagt accttccaga ataaagtaat tggcgaagta ttgaaagacg ctaatgtaaa
     1081 aatcaaagac aaagactttg aaaatgtttt gagtacattc acttcagatt ctagtacagc
     1141 aagtagtaaa taagcggtat aaataaaaaa tatgaactat ttaaatcaat aaaactgttc
     1201 atctaaatct ttttattaga actaaatcaa cagtag
//
LOCUS       DACEMO010000332         1234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.342, whole genome shotgun sequence.
ACCESSION   DACEMO010000332 DACEMO010000000
VERSION     DACEMO010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.342"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(44..1027)
                     /locus_tag="IW728_002758"
     CDS             complement(44..1027)
                     /locus_tag="IW728_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1026077.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          343 a          268 c          236 g          387 t
ORIGIN      
        1 cagctttttc acgacctcta attcacggtg ttcaagcaag aaatcattgt tcactgaaaa
       61 gcgttggatg ttctttttca aaggtctcta agatctctcg tacttcatca gtagatttcg
      121 tattcatcaa ctgaactcgc aagtcatttg ctcctggaaa tcctttgata tagattttaa
      181 agaaacgatg tagtcctgtg atggaacgcg ggatgatttc tgaataatga tcttgcagat
      241 ctagttgcag acgaagtagt ccgatcagtt ctttttctgt atgttccctc gggtcttttt
      301 caaatgcata aggattcttg aaaatccctc ggccaatcat gatgccatca acgccatatt
      361 tctcggccaa ttcttctcct gtctggcgat cggggatatc tccattgatc gtgattagtg
      421 tttgtggagc gatttggtca cgtaaagcaa tgattttagg aatgacttcc caatgtgcat
      481 ctactttact catctcttta cgcgtacgca aatggacaga aagattggct atatcttgat
      541 gaagtaggtg actgatccat tcttccatct cagacatttc cgtatagccg atacgcgtct
      601 tgacgctgac aggcaaaccg ccagctttag ctgcttcaat caattgtgcc gctacttctg
      661 gacgaaggat caagccactg ccttttccac gttctgctac attagggact gggcagccca
      721 tgttgatatc gattccttgg aagcccattt ctgccatagc aatgctcatt tctctaaaaa
      781 attctggctt gtctccccaa atatgggcaa ccatcggctg ttcatcttct gtaaatgtta
      841 atcttcctcg tacactgtct tttccatctg gatgacagaa actgtctgag tttgtaaact
      901 cagtaaagaa gacatccggt gctgctgcat gctttacgac atggcgaaat acaacatctg
      961 ttacatcttc cattggtgct aaaacaaaaa aaggctttgg cagctctttc caaaaattag
     1021 tagtcattta tttgttactc tccattctat caagcagtga agtcgcaatc tttctcaaaa
     1081 acattgtcat tatactagga aacaaaaaaa gaagcaaagt gaacagctta aaagatgttt
     1141 ctgattgagc aaaaaaaaga ctgtgacaaa aatcttatca cagtccattt tccggaataa
     1201 atggtgttca aaagcttgca tttgcggtaa taag
//
LOCUS       DACEMO010000333         1216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.343, whole genome shotgun sequence.
ACCESSION   DACEMO010000333 DACEMO010000000
VERSION     DACEMO010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.343"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..295)
                     /locus_tag="IW728_002759"
     CDS             complement(<1..295)
                     /locus_tag="IW728_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1026078.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRF"
     gene            complement(355..858)
                     /locus_tag="IW728_002760"
     CDS             complement(355..858)
                     /locus_tag="IW728_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1026079.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(871..1092)
                     /locus_tag="IW728_002761"
     CDS             complement(871..1092)
                     /locus_tag="IW728_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026080.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(1097..1216)
                     /locus_tag="IW728_002762"
     CDS             complement(1097..1216)
                     /locus_tag="IW728_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1026081.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT          414 a          307 c          165 g          330 t
ORIGIN      
        1 taaatcgtaa gtgatagcta ataaaagatc gtaaaaatcc caatggtttc ttaccgtcaa
       61 acgttttttg cgacatgaac caagtaactg ccaccggtat acctaagtat tttaaaaagg
      121 ccccctctat catagatagt ggaggcacat gggcaaatac gatgacaaac agcaaggata
      181 aaacaaacca agccatctgg ttaaaagtca caggaaaagg cagttgaaag tcattaattg
      241 cataaatgac cttttcaact gcccaaatac tggtgtagct ttttattttc ttcatcaaat
      301 cattccttta aaaagaagga tagtttagaa atcaactacc cttcaataat tttgttaata
      361 tggatactca aaaacacctc gactcgttac caaataattg cctccaatct ccaagtctct
      421 accacacgca tcaaaatcaa tatacgtctt caagtgttct ggtacttctc caaaatcacc
      481 agcttctaca atcaagaaat atgctacatc actcatatca tcgcaatctg gataatagat
      541 aatatcatcc acatgctcta ccatctcctc aaaagaattg aagaaagcat gttgaatctc
      601 agaagcaact tcaccaactg gcgttccttc aagttcctcc gctaagccac acaaccgatt
      661 aatttcctca attggcgtat actcatcaat ctcaaaggga agttcataat catgaattgc
      721 atattcctcg tattcgtcat ttaacccaat tcgttctttc acttcatcaa aatcgacagg
      781 tggggtaaac cacgccccca ccaattcacc ttcgttatat ttcccgaggt tcgcaatata
      841 aacacgcatc tgttccatcg tgcttccccc ttacgcaccg acaattcggt taaacaaact
      901 gagtaagaca tccttcaccc cagaagcatt aaagaccaat ccgaccgcaa tcaaagcaat
      961 caccaaaaag ccaatcagtt tggaaaactc ccgcttaaat cctaaaaaca aaccaatgac
     1021 cacaattgcc agtaagacta aggattgggc attggttaaa aaccattgat ataaattttg
     1081 tccgaaattc atcttgtcac tttcctttct tttccattgc tcgatccgct gctgcacttg
     1141 cattcatcaa gcacatcaca gtaacaccaa gcatactgcc accaaatacc aaaaaaatat
     1201 cttttacaat tcccat
//
LOCUS       DACEMO010000334         1202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.344, whole genome shotgun sequence.
ACCESSION   DACEMO010000334 DACEMO010000000
VERSION     DACEMO010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.344"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(34..405)
                     /locus_tag="IW728_002763"
     CDS             complement(34..405)
                     /locus_tag="IW728_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026082.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(407..808)
                     /locus_tag="IW728_002764"
     CDS             complement(407..808)
                     /locus_tag="IW728_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026083.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(822..>1202)
                     /locus_tag="IW728_002765"
     CDS             complement(822..>1202)
                     /locus_tag="IW728_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026084.1"
                     /translation="SEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEI
                     EDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAV
                     VKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          379 a          218 c          154 g          451 t
ORIGIN      
        1 aaaaaaataa caaacaaact cgcttgaaat tcattaggtg agtttgttta ttattttttc
       61 tgcttctcga ttcatcagcg ccatatctgt tttcgtaatg gtttcattag gaatatagtc
      121 agcacatttt cttttctcta acattctata cccactatta tgtattccta aggtattcaa
      181 ttccttaact gttgaaaaca aattccaaac ggtttggtgg cttccttttc cgctacttcc
      241 attataaata atatcataat tttctttcaa atactcatca gattttttaa aagcactgta
      301 gtatgctctt gaaacagcag ttcttctata agattcttta gaattatatt tatcatctga
      361 ttcaatttgt ttggaaatag ataaatattc attaaagtca aacattttat tcccccaaag
      421 ttgctactac agatttcccc atatatttat agtcagataa tacattatcg agagtatcca
      481 aaacatcgta atctgaggaa aacaattcag atgttgtttt gataacaaag aaatacaagt
      541 ttaaatcttc atcatatttt atattgattt cagcttttct ttttaaaacc gtatttatca
      601 catcaaaaat tttgccatca ttacttgaaa ttgtgttaat ctcatttttt gtagtaatta
      661 caagttgatt atcattttta gttttcggag tagaacttgc atccacaaca actggagatg
      721 ctagaattac cgatgcgcaa gcaaaaatcg cagcaaaatt tattaagcta ccactccgat
      781 gtgtatttga tggtactata tcaagcatcc gtatcgtctc cttaattttt ttccactggt
      841 ttgttgacct tgatatcaaa gatttgcctt ccaatctcag ttccattaaa tttaacaaca
      901 gcataatgct ctcctgcttc ttcaaccaag acatttctgg cgtttatatt aaagtttata
      961 tcgttaactt gaggaggcaa gcttaccgat acttcacccg tagaaaaaat ttgtttttca
     1021 ttcttgttta aaacttcaat ttctactatt tttatctttt ctgtatcctc tatctcagac
     1081 accacagctg ttacagaaaa tgataaagca gtaggaatta aaggcgcatt aatagaaaga
     1141 gctggattaa ttactttatt aataacatta gtgccaaatt gctgactttc tacatcttct
     1201 ga
//
LOCUS       DACEMO010000335         1194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.345, whole genome shotgun sequence.
ACCESSION   DACEMO010000335 DACEMO010000000
VERSION     DACEMO010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.345"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(105..905)
                     /locus_tag="IW728_002766"
     CDS             complement(105..905)
                     /locus_tag="IW728_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026085.1"
                     /translation="MFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKE
                     KNYLAILRLSCNQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTK
                     VLYQEWLRYVIGIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGM
                     LLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNK
                     PPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
BASE COUNT          338 a          238 c          215 g          403 t
ORIGIN      
        1 atgtgggaac ttctaatttc tttaattcca atggatttcc tcctttacta atcgcttttt
       61 attatagcac agactgactt gttttgatct tttttccaaa ggttttatcg gtttttgcct
      121 aaccagtaat tgatatcggt aagaagtctt tcgataccaa atgaaggatg gtacagatac
      181 gttttttgat cttcgttatg gtctagtgga aaaaaatgat cggtgatcac tagatctgca
      241 taccgaggcg gcttatttcg agtacataca tgtatagaga tccgttttcg atgtcttagc
      301 gttttgttca gtaccccagc aagtcttctt gcttcttggt gttcatagcg aaaaaaaaag
      361 acgattttca cttcaggagg gtggacatct aaataccctc gttcctggag tgttttcagt
      421 aaaagcatcc cccactctac tgcggggtgt gcaaatgctt gttggatcgc tagccgaaat
      481 tgatatagtt tatctttgga attgggaaat ttttctaatg tttcctgata ataacgaact
      541 tcttctatca gccggtcatg gataccaatt acgtaacgaa gccattcttg gtaaagtact
      601 tttgtcagct ttgatttgtt ttcgctctca aattcgctat tcagccaaaa cagcgaatca
      661 agcttttcag tcacaatcgt caatttccga tagtcttgtg tctcttctat ccgtttccac
      721 cgcttttcag taatctggtt acagctcaag cgcaaaattg ctagataatt tttttctttt
      781 attggcaaaa tctgctcttc tctaaatagc aattcaggaa acaattcaaa gaatagtgtt
      841 cgactgtctc ctaaaatctc gatttctgcc ggatcctttc tttgcttcaa taattggatg
      901 aacatctcag aaaaggactg ctctccatca tctgcaaaaa aatgcggaaa atctttcaaa
      961 aagcaaaaca gcagatatct tacttttctt tctgaaccaa agatctgtgc ttttttataa
     1021 gaaaaagaaa ggtggtactc gccaaaccac tttctcaatc gctcttgcgt agaacgatcg
     1081 tacttctgct cgtaaggttc attcaatagc agatttttca gtaaacaaaa cgggggtgat
     1141 tggatcagaa aaacaaacaa gagataaggt tcgctatatc cttgatcata aact
//
LOCUS       DACEMO010000336         1194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.346, whole genome shotgun sequence.
ACCESSION   DACEMO010000336 DACEMO010000000
VERSION     DACEMO010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.346"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..854
                     /locus_tag="IW728_002767"
     CDS             <1..854
                     /locus_tag="IW728_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ1026086.1"
                     /translation="ISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLV
                     LFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRK
                     VEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDF
                     YGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFS
                     LLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLY
                     LKKGELI"
     gene            851..>1194
                     /locus_tag="IW728_002768"
     CDS             851..>1194
                     /locus_tag="IW728_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026087.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDV"
BASE COUNT          399 a          143 c          196 g          456 t
ORIGIN      
        1 taattagtgt attaatttca aaaataacaa aaacgattcg tgttttttat atgatgaatt
       61 ttatcgtaca aatattttat attacacttt cttcgtctag aggtgcccag gtagtaattt
      121 gttctttttt agtattattt cttttgttgt ttgccaattc aaaatttaga aagagcatta
      181 gagaagtact acctgtaaaa atcaaatgga tggtaagcat attattagtt ctattggtat
      241 atttttcttt tggtactaat tatgctaagg attttttagc aacaattcct gtatcgattg
      301 atagaaaagt agaagttata gagtcaaatg aatcagataa aactccactt ccagaaaaag
      361 aagtaatatt acagcattct agtgataact ccgaaatttc agctggtcgt tttacgattt
      421 ggaaagctgc gcttaaagtt ataagaccaa ctttgttatt tggttatgga gagaccgatt
      481 tctatgggaa tagcgatgca aaatttttga aaaaagtaga tcttgatact acagatgtga
      541 atgaactaaa aagagcacat ggaaacatgc ataatggatt tcttcaagta cttgtatcaa
      601 gtggtataat tgcttttata tgtatctatg ctttctatgt gttaaatatc tttagtttac
      661 tcaaaacatt ttttagaagt aaagtgaatt atgcgtttct tggtattgtt ttaattttta
      721 tactatctat ttttatagat gaaatggtgg aggctcatgt acttttcaat aagcgcgatg
      781 tcatttcaat tgttttctgg tattatttgg gaagcatttc ttacttgtat ttaaaaaaag
      841 gagaattaat atgaagacag ctttcattgc ttggactcct ttacatttga ttaatatatt
      901 aaatgtttat caaaattttt accgagaatc aaaaggagat ttatttattt attctgaatt
      961 tgaaaacgct agaagatata gtgagcgtat aaaagaatta gggctattta atgatgttgt
     1021 ttttgtgaat caccggaaaa tcggttcttc ctttaaacgt aaaattaact tgttaactaa
     1081 ccataatcaa tttttctcgg atgatttatc cgtttatgat gaaattttta tacaggggga
     1141 aaattatttc acaaaaatac ttttctccaa tgtttacaaa aaaaatccag atgt
//
LOCUS       DACEMO010000337         1187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.347, whole genome shotgun sequence.
ACCESSION   DACEMO010000337 DACEMO010000000
VERSION     DACEMO010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.347"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..835)
                     /locus_tag="IW728_002769"
     CDS             complement(<1..835)
                     /locus_tag="IW728_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ1026088.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NE"
BASE COUNT          357 a          219 c          179 g          432 t
ORIGIN      
        1 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
       61 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
      121 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
      181 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
      241 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
      301 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
      361 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
      421 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
      481 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
      541 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
      601 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
      661 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
      721 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
      781 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
      841 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
      901 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
      961 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
     1021 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
     1081 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
     1141 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagata
//
LOCUS       DACEMO010000338         1166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.348, whole genome shotgun sequence.
ACCESSION   DACEMO010000338 DACEMO010000000
VERSION     DACEMO010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.348"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            62..472
                     /locus_tag="IW728_002770"
     CDS             62..472
                     /locus_tag="IW728_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1026089.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            486..842
                     /locus_tag="IW728_002771"
     CDS             486..842
                     /locus_tag="IW728_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1026090.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          414 a          167 c          222 g          363 t
ORIGIN      
        1 taattggtat tttaaaagaa attactgtag aattggatta taattaaaaa ggagtgagaa
       61 aatgataaaa gtatatactt cgcctagctg tacttcctgt agaaaagcaa gagcatggtt
      121 gcaagcgaat caacttgaat ttgaagagaa gaatattttt gccgaaccgt taacagaaag
      181 tgagatcaaa aagatcttgg cagcgactga aggaggagtt caagatatta tttccactcg
      241 gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa ttaactttaa agcaaatgat
      301 cgtcttattc aaagaatatc cttcattatt gagacgacct attttgattg atgaaaaaag
      361 aatactaatc ggatttaatg aagatgagat ccggtcattt gtccctcgtg aaattcgatg
      421 tgttgccaga aatctgatct atttacaaaa caataacatc ttaaattcat agggagggag
      481 ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt aacaagtctg aaggcgaaac
      541 aatggttaga acaccatcaa ttgccttatc aatttgttga cttagaagaa cgcgatctaa
      601 gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa cagctatttg
      661 cgacttggtc tgaggctttt cacaacattg cgattgacct ttcctgtgta ggaaaaaaac
      721 aactgatttt tttatgcaca caacatacca atctcctcag acgtcctttg attattatcg
      781 atgaagttct atttattgga tacaatgagc agttgatgga acagcttatc ttaaataaat
      841 agactttaac tgttttaatc aagtcactga gcatttcata cagttgtaac agtcgaacac
      901 ttagcatcaa gcgtgttatg tgttcttttt ttgactttat ttttctacta tagagacatg
      961 ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa aatatattga
     1021 catgtgtaaa tttacatgat atattcattt ctgttgtttt aagtagcaaa gcaataaaat
     1081 agtattttaa aaaacatata aaatagttgt tgacatgtta gaaatgacat gataagataa
     1141 ctgagttgtt aaaaaagaac cacttg
//
LOCUS       DACEMO010000339         1161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.349, whole genome shotgun sequence.
ACCESSION   DACEMO010000339 DACEMO010000000
VERSION     DACEMO010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.349"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            84..>1161
                     /locus_tag="IW728_002772"
     CDS             84..>1161
                     /locus_tag="IW728_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026091.1"
                     /translation="MTRLLIQPTFIRSFTQQADPYDLRKLQEKIMLASVRDESWLVVG
                     NDENECSFRLEDNQLLIKNVLSISVFKEKQFLIRDFIQKKMIAHGVFAYMRAYSEFIY
                     HNTKQISQRLLFEKKDEIEHLPKMKQQNGEVVVDCNQFPGYDLFYQGLCFTSCWEMYY
                     SRYYHQIIPKPIFLDIQQVEKVKELENEVICIQLYRDPFNWQKPNNQMFQSYFRDQLG
                     FDHLAWDNGVGLLKPPFVEYIYTDHTIQSVQYQNAQMQPVPKKNASFFVTKSYDIQQG
                     DYKERRVRGTLNAQAYFPWVDENRSRMMCYKVIDPTVALDNGIEAYCYYIREYLEVEV
                     TDEKYQEYLLSLRIYVPSESLSELP"
BASE COUNT          395 a          202 c          222 g          342 t
ORIGIN      
        1 gcgaagataa attattgatg actatccagc tcacagactt aaatgaagaa gaaaaaaaac
       61 aggaaataag cgattgggct attttgacac gtctgttgat ccagccaaca tttatccgct
      121 ctttcacaca acaagcggat ccgtatgatc ttcgaaagct tcaagaaaaa ataatgctag
      181 catcggtaag agacgaaagt tggttagttg taggaaatga cgagaacgaa tgtagttttc
      241 gcttggaaga taatcaatta ttgataaaaa atgtattgtc tatctctgtt ttcaaagaaa
      301 agcaattttt gattcgagac ttcattcaga aaaaaatgat tgctcatggg gtgttcgcct
      361 atatgcgagc ttatagcgag tttatctatc acaatacaaa acagatctct caacggctat
      421 tgtttgagaa aaaggatgaa atcgaacatt tacctaaaat gaaacaacaa aacggggaag
      481 tagtagtcga ttgcaaccaa tttccaggat acgatctttt ttaccaaggt ttatgtttca
      541 cttcgtgttg ggaaatgtat tatagtcgct attatcatca aatcatccct aaaccgattt
      601 ttctcgacat ccaacaagta gaaaaagtga aggaattaga aaacgaagtc atatgtatcc
      661 agctttatcg agaccccttt aactggcaaa agccaaacaa tcaaatgttt caatcctatt
      721 ttagggacca gcttgggttt gatcatcttg cttgggacaa tggtgtcggt ttattgaaac
      781 cgccattcgt tgaatacatt tacactgacc atacgatcca atctgtccag tatcagaatg
      841 cacaaatgca acctgttcct aaaaagaatg cttcattttt tgtaacaaaa agttatgata
      901 tccaacaagg agattataaa gaaaggcgtg ttcgcggaac actaaatgct caggcgtatt
      961 ttccttgggt tgacgagaat cgatcacgca tgatgtgcta taaagttatc gatccgactg
     1021 ttgctttgga taatggaatc gaagcttatt gttattatat tagggaatat ctagaagtag
     1081 aggtaacaga tgaaaaatat caagagtatc ttttatcctt aagaatctat gttccttcag
     1141 aatccctgtc agaattaccg t
//
LOCUS       DACEMO010000340         1146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.350, whole genome shotgun sequence.
ACCESSION   DACEMO010000340 DACEMO010000000
VERSION     DACEMO010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.350"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            120..1106
                     /locus_tag="IW728_002773"
     CDS             120..1106
                     /locus_tag="IW728_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1026092.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT          447 a          180 c          251 g          268 t
ORIGIN      
        1 attaaattgt tttaatcaaa ttccaatcta aatatttgta gaaaacaatc gtctcctata
       61 gaataaattt ataagtaaca atataccaat cataaatcta tttgctagga ggaatttaca
      121 tggaagagaa aaatttaacg gtcatcggaa gctatagtac gagggaagaa gcattatcag
      181 taattgaacg gttgcgtaac gaagggtatg aacgagatga tatcgttatt tatactactg
      241 atgaagcagc atctagacta ggatttgacg gattgtctgg tatagatgtt gaaacagatg
      301 aaaatcggat ggatggtgaa gaagaccgtt ctttatggga aaaaataaaa gatacttttt
      361 cttttgatac ctatgattct gagacggcta caccagaaaa tgatccttta tatcaatata
      421 gaggagatat ttctgatgga aaatttgtca tcacagtaaa aggctatcgt cagccagaaa
      481 caacagaaga tacaatggat acacaagata ctgttagccc agcaagcaca atggggactg
      541 gattagaaac tggcactgga acagatccaa tggacgtagg tggcacgaca ggctttcaaa
      601 atacaacgga tccattgaat atgacaccag gaactgatcc gatggacgta cctggcgaga
      661 ctgatacaat gggtacaagc aagactgaag acatcgatcg caaaccaaat ctagatgatg
      721 atacaattca actaaaagaa gaaaaattag atgtaaatac acatgatgtg acaactggag
      781 aagtcgacat ccacaaacat gtggtcaacg atactgaaac ggtagaagta ccggtcaaac
      841 gtgaagaaat cgtcatcgaa cgcaaaccag taacagatca atcttcacag ggaacagacg
      901 aaaaattgga agatgacacg attactatcc caatcaaaga agagcaagta gatgtaagca
      961 aacatacagt tattcgtgaa gaagttggga ttcataaaga agaacatgaa gatgttgaaa
     1021 aagtgacaga agatgttagc cgagaagaat tggatattga cacaagcggt gatgtacaca
     1081 ttgaagaccg caataaaaaa tcgtaagcgt aagctaaaga ggttgtgaca gaagtggtca
     1141 gcttaa
//
LOCUS       DACEMO010000341         1119 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.352, whole genome shotgun sequence.
ACCESSION   DACEMO010000341 DACEMO010000000
VERSION     DACEMO010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.352"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            106..1056
                     /gene="yidC"
                     /locus_tag="IW728_002774"
     CDS             106..1056
                     /gene="yidC"
                     /locus_tag="IW728_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1026093.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT          377 a          223 c          232 g          287 t
ORIGIN      
        1 aaaagaacga atatttatat gaaatttcta tgaagttgat tgaattccat gcgctttttt
       61 agtatagtta agtttgtgta aaaattatag aagaggaagt attctatgaa taaatggaaa
      121 aactggttat tgggatccgg acttgtcagt atcctgctct ttttatctgg ctgtgtgcgg
      181 atggattcaa acggaaaccc tgacacttca ggaatcgttt atcgagtact cgtccatcct
      241 atgggccaag cgattacata tctagttgag aatttcaatt ggtcttacgg ctgggcagtt
      301 attgcaatga ctgtgatcgt tcgtatcatc atcttgccac taggtatcag tcaatcgaaa
      361 aaaacaatga tccaatctga aaaaatgcaa gcgttaaaac cacaagttga agcagcacaa
      421 caaaagctaa aagctgcaac aacacgtgaa gaacaaatgg cagcacaagc agaaatgcag
      481 caagtatatc gcgaaaatgg attgagtatg accggcggaa tcggctgtct gcctctattg
      541 atccaaatgc cgatcttctc tgccttgtat tttacggctc gctacacaga aggtatccgt
      601 gaatcatctt tttacggcat cgatctaggc tcaccaagta tggtgcttgt ggccatcgca
      661 ggggtcgctt atctgcttca aggatatatt tcaacaatcg gtattcctga agaacagaaa
      721 aagacgatgc gtacgatgct gatcgtctct cctgctatga tcgtcttcat gtcgatcagt
      781 gcgccagccg gtgttacact ttactgggta gtcggtggtg tgttcagctg tctgcaaaca
      841 ttcatcacaa acgttataat gaaaccgcgt ttgaaagcac aagtggcaga agagttgaaa
      901 caaaacccgc caaaacaagt cgttactcca agaaaagacg tgacagaatc agcaaagcca
      961 gctgacaaca aacaagcaac aaaacaaatc aaacaaccaa aaaacagtac aaacaacaaa
     1021 ggccgcaacg caggaaagca gcagaagcgg aaataaaaat atatgacaaa aaactgtaga
     1081 caaataaaaa aatttctttg ttctacagtt tttttgtta
//
LOCUS       DACEMO010000342         1102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.353, whole genome shotgun sequence.
ACCESSION   DACEMO010000342 DACEMO010000000
VERSION     DACEMO010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.353"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..157
                     /locus_tag="IW728_002775"
     CDS             <1..157
                     /locus_tag="IW728_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1026094.1"
                     /translation="RYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDT
                     LVSMFTE"
     gene            494..733
                     /locus_tag="IW728_002776"
     CDS             494..733
                     /locus_tag="IW728_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026095.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          380 a          201 c          165 g          356 t
ORIGIN      
        1 aagatacagc cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt
       61 cagcattttt gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca
      121 ggtaaccgat acattagttt caatgtttac tgagtaacta attattttgc aggaggacaa
      181 tttatttaca caaaattatt gacgctccca gctatcctcc tagcaactat ttaaataccc
      241 aaaaaaatta cctctagata atatcatacc tacgttttca cttataaatt ttttcataaa
      301 aaaagcgttt agaggcataa tcgttaatac agatggtaac caatgtatct ggtttgaact
      361 gggatatcac cctctccata tatgtattac taagactaat agatactata aaagacttga
      421 tagttttgca ttgattttgc ctaatactga gcataggtgt atgctaaacg atactataag
      481 gagtgtgtac ataatgaaaa gaattctgtt tatcagttta tcaaatttat tgttacttac
      541 tgcttgtggg acttctgcat caaagaacat accttcagat acgcaaattt cgaatagtat
      601 cgattcaaca atttctacaa atgagaaaaa ttctccggta gaaacttccg atataaaagt
      661 ttctacctct gcaaaaaaca gggtaaagaa actgtcagca cgtctccttt atttcaaaca
      721 aactctgaat tagcttcttc atcttcttca gcaaataatg atgatagttt cgcgtttact
      781 cctaactaaa tgcaaccttt acaaatgctt tacgataaca agcctgagct aaatgatcct
      841 gatatcagtt ttgcattttt tgaaatgatt ggatcggatg atctttttaa aactactagt
      901 ttgtctatcc gaaaatagga cagttctggc agtataggtt tttatcgagt ttctccacca
      961 ggcctcgtta cagagacaga ttctctaggc gaaccttttg aataaatcaa aaatcatgaa
     1021 caatcactgc caactaaata aagtgccttc aaaagcttgg tcttctcgct tttgaaggca
     1081 ctttatttaa atctttatga aa
//
LOCUS       DACEMO010000343         1098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.354, whole genome shotgun sequence.
ACCESSION   DACEMO010000343 DACEMO010000000
VERSION     DACEMO010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.354"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            191..445
                     /locus_tag="IW728_002777"
     CDS             191..445
                     /locus_tag="IW728_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1026096.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IW728_002778"
     CDS             446..763
                     /locus_tag="IW728_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1026097.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          388 a          170 c          221 g          319 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataa
//
LOCUS       DACEMO010000344         1085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.355, whole genome shotgun sequence.
ACCESSION   DACEMO010000344 DACEMO010000000
VERSION     DACEMO010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.355"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..635)
                     /locus_tag="IW728_002779"
     CDS             complement(81..635)
                     /locus_tag="IW728_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1026098.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          355 a          194 c          116 g          420 t
ORIGIN      
        1 cttccgaaaa ttcagttatc ttcattaatc tcaccttcta agttttcatt atacacaaaa
       61 aatatatcct aatatctcaa ttagcctccc aatttaaaac cacgtaacga tgcccctatt
      121 tcatcttcag ttataccaat gtaccttttt gtaattgctt gcgagctatg attaaaaata
      181 aacattaagg tagcaatgtc tctagttttc ttgtagtagt gatacccaaa agtttttctt
      241 aatgtatgtg ttcctatatc atctcttcct aatttttctg ctacatctgc atatatctga
      301 tagacacgat cccttgttat atgtcgacct tgttgccgac ttggaaataa ccaatcattt
      361 tcttctttac cttcagtata taataaaatc tcttctctca aattatctat aaaaatcggt
      421 tttcgcttac ctgttttatg ttcaacaata ataggcttac ttttacctcg tatgtctcca
      481 acttttaatc ctataatatc tgacatacgt aacccattat tgattccaaa caaaaataaa
      541 aaactatctc tttctggatt acttgttctt cttagccaaa actgaaaatc ttgtatctct
      601 tcatttgtcc ttaacggttg tacattatag ctcactattg tttcccttct taaaaatatt
      661 ctgagttcat gattatatat atattttacc ataatcatga acttatatat atactctgtt
      721 aaaagtgaag aaaatcaagg ggtttagttt atgattttat atcatttcag taactcagaa
      781 tattccgact attttgtaca aaaaaattaa ccttacttat atgaagattc taagctctta
      841 attaaatttt agacgggcag ttatccgtag aagttcttag aatagctaat ttacataatt
      901 aaattatatg aaatttaaat ttggacaata tactaagaac tccccttact ataaagtgaa
      961 ataaattagc atatgaataa ccgtgctacc aagcttttca ttaataaaat atttttatta
     1021 atattttata actttacgca agatcataat tttgtctgtg tagatatttc gttatatcac
     1081 taaag
//
LOCUS       DACEMO010000345         1083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.356, whole genome shotgun sequence.
ACCESSION   DACEMO010000345 DACEMO010000000
VERSION     DACEMO010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.356"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1083
                     /gene="fss3"
                     /locus_tag="IW728_002780"
     CDS             <1..>1083
                     /gene="fss3"
                     /locus_tag="IW728_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1026099.1"
                     /translation="EGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIAT
                     LDEIPHGTKVTITEKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGV
                     ETGSDLWNDNYSLAGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYV
                     TETKASHGFVNTFKPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKA
                     QGKAVFEGAEYTLFTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHL
                     AINEYYWQETKAPEGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFK
                     FAGSAAGTTETGFNDLTFKVSPLEGTN"
BASE COUNT          377 a          202 c          249 g          255 t
ORIGIN      
        1 gagggttctt taaagatcaa aaaagtcgat aaagaatcgg gtgatgttgt accaggaacg
       61 gttttccatt tagactttgg aaaaacttta cctgcaaaag acgtgactac tgacaaagaa
      121 ggcattgcta cattggatga gattcctcat ggtacaaaag taaccattac cgaaaaatca
      181 gtaccggcgc cttatacgat tgataccacc cccatgacta ccaccattaa agcaggcgaa
      241 accatttacg tgacttcaaa aaacgcacgg gaaaaagggc aaatcattct ggataaaagc
      301 ggagttgaaa cagggagtga tctttggaat gacaattatt ccttagctgg taatacgttt
      361 gccattcgca aagacagtcc aaccggtgaa atcgttcaag aaatgaccac ggatgaaaac
      421 ggtcatgcgg aaacaccaaa agagattgct aatgcgttgg aattgggaac ctattatgtg
      481 accgaaacta aagctagtca tgggttcgtg aatacgttca aacctgtaaa agtcgaattg
      541 aagtatgcca atcaaaccgt agcccttgtt accagtaacg taaaagggca aaatcaagaa
      601 gtcactggcg aaaccacttt gacgaaagaa gacaaagata ctggggataa agcacaaggt
      661 aaggcagtct ttgagggtgc tgaatacacc ttgtttactg ctaaggatgg aaaagctgtt
      721 aaatggagtg aggcttttaa accagagtta gcgaagggaa cgaaaggttc tgatgaaacg
      781 gtcactttga ctttagacga aaagaaccaa gttgccgtta aacatttagc tatcaacgag
      841 tattattggc aagaaacaaa agcgcccgaa ggatatagcc ttgatgaaac aaagtatcct
      901 gtatccatca aaaaggtgga tgatagcgaa aaaaatgccg tgattacccg aaatgttacg
      961 gcaaaagaac aagtgattcg ctttggcttt gatttcttta aatttgctgg atcagctgcg
     1021 ggcactaccg aaactggctt caatgatctg acctttaaag tgtcgccatt ggaagggacc
     1081 aat
//
LOCUS       DACEMO010000346         1049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.357, whole genome shotgun sequence.
ACCESSION   DACEMO010000346 DACEMO010000000
VERSION     DACEMO010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.357"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            153..>1049
                     /locus_tag="IW728_002781"
     CDS             153..>1049
                     /locus_tag="IW728_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1026100.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVN"
BASE COUNT          349 a          176 c          215 g          309 t
ORIGIN      
        1 aacaaaaaat atgagtagct atttttttgg gctgtactta ttacttgatg caggacagct
       61 ttttcacaac ctcatcggct ttcattttct atttttttcc tgaacccata taataaaaga
      121 aagaacaaaa aaacttgcaa gggaggacac atgtgaagca gagtaagcaa aaacagtctc
      181 gtcgactttt gtttcgtttg tttttattaa tatgtatcgt cgtagggtta ctgatcgctt
      241 tatatccatt ttatgtggat tctttaaata gttttattga tcaaaaacgt atcgaacaag
      301 tacaaaaacg gaccgcagct gaaattgaag tgcagcgaaa gaaaatggag gagaaaaacc
      361 aacgattaac tgaccaagga ttcaatcctg gtactgatcc tttcaatgaa aagaacagaa
      421 atgaaagtac aacatccagc caattagagg aatggctgat cggaagtatc aatataccta
      481 agatccaatt aaatatttca ctttatgatc ggctcaatgg taggatcctt gaaaatggag
      541 caggcgtctt gcaaggaacc tctttcccgc ttggtggaaa ttcgacgcat tcagtgatct
      601 ctgctcacag cggtttacct aatcgacggt tatttaccga attagatcgt ttagaaaacg
      661 gagacacgtt tattttgact gttttggggg agaaattggc ttatcaagtt aaaaatatac
      721 aagtggtact gccagatgac acgtctgttc taacgatttc agaaggagaa gacttagtta
      781 ccttgcttac atgtacgccg tatatgatca acacgcatcg tttgctagtg acaggacatc
      841 ggattcctta taacgaatcg gtaaaaaaag aagaaggaaa aggaaatcag gaaagaacca
      901 tgcggcaatg gtttattctt gcaggaacga taatagctgt agtcatactg ttagtgttta
      961 tcggacgact aatctatcag tatagattaa gtaaaaaagt gcttgatttt tcatttatta
     1021 ttagtgattc gtcagggaaa ccggtaaat
//
LOCUS       DACEMO010000347         1046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.358, whole genome shotgun sequence.
ACCESSION   DACEMO010000347 DACEMO010000000
VERSION     DACEMO010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.358"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            355..>1046
                     /locus_tag="IW728_002782"
     CDS             355..>1046
                     /locus_tag="IW728_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1026101.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYS"
BASE COUNT          405 a          147 c          164 g          330 t
ORIGIN      
        1 gtatatgatc taagtcttgc ttctagtatg aatatgaccg atgtatacga aaagttatta
       61 atactagaaa atgaatatga tcaattcaat tcaaatcagc agcgaaaagt aaagaaacag
      121 atagactaga atagctattt tcgaaaagca atatttaagg tataagttat acctttaata
      181 ataaaagtat aagttatacc ataaatatat atggtataac ttatactttt attattaaaa
      241 accgctatat ccaaacgttt ttagatatta ttatttaagt tatactttta atgttgacca
      301 tttccgatat ataagttata ctttaattat caattaaaga ttgggaggat ttacatggat
      361 acttttcaag ggactacatt tacagttggc aattttaaag gaggagtagg taaaactaag
      421 atagtcagca tgcttgctta tgacaatgca atgataagaa atagaaaaac gcttattatt
      481 gatttagacc ctcaagcgaa cttaacacaa gtcatagcaa gaactttcgg tataaccgat
      541 atcaaaacca ctataacaaa tggtgtcgca aatggagaat tagcaagtag tattattaag
      601 gtaaatgaaa atcttgacct tctggcatgt gacactagtt ttcgatcatt ctccgctttt
      661 gctaataaac atgaaaatga attagaccaa gttactgtct tgaaaaaatt acttcttcca
      721 ataaagaaca attatcaaga aatatttatt gatgtaccac caaccattag cgaattttcc
      781 gataatgcta tggcagcttc agactattcg ataatttcat ttcaaacagc tgaagaatcc
      841 ttagagggag tcaacaaata tgtaaattat caaaaattca tggtcgatag atacgatctt
      901 gatttgcaaa ttatagatat tgtaccttgt atggtagaac caaatgatga ttttgatgaa
      961 gaaatcctag aagaagcaat tgaaaaatac ggaaccgtgg tatccaaaaa tctcattcat
     1021 tatcaaaaaa gattgagaag gtattc
//
LOCUS       DACEMO010000348         1036 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.359, whole genome shotgun sequence.
ACCESSION   DACEMO010000348 DACEMO010000000
VERSION     DACEMO010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.359"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..>1036)
                     /gene="cls"
                     /locus_tag="IW728_002783"
     CDS             complement(81..>1036)
                     /gene="cls"
                     /locus_tag="IW728_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1026102.1"
                     /translation="GVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRF
                     RLNYHDHRKIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRF
                     LTDWNVSVPEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKL
                     ITSAKKRVWIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQ
                     LIKENVQILVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEE
                     LATQFKEDETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT          310 a          187 c          200 g          339 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgcctt
//
LOCUS       DACEMO010000349         1013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.361, whole genome shotgun sequence.
ACCESSION   DACEMO010000349 DACEMO010000000
VERSION     DACEMO010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.361"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            202..675
                     /locus_tag="IW728_002784"
     CDS             202..675
                     /locus_tag="IW728_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1026103.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            684..911
                     /locus_tag="IW728_002785"
     CDS             684..911
                     /locus_tag="IW728_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1026104.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          347 a          151 c          167 g          348 t
ORIGIN      
        1 agaagagaca cacatagaat ctaaaataac tggttttaaa tgtttcttac aatcaaaaaa
       61 agctgttaaa tgcactccac accttcatac tctatcattt tttataaacg atatattttt
      121 attgtttttc gataaaaaat agttgtttta caataattat tctgctatat tttagctatc
      181 aaagataaaa gagggataaa aatgaaattt aaaacattgc tattaaaatt tgtcgtttta
      241 ggtataagtg ccgtatttat tttgttcggc gtactttttt atactcagat cgctacaagc
      301 gaaaaaggat tcaattttga ttggcagacc acattatttg tttctgtcat ttactttgcc
      361 cttctcctag ggatcgttat tgcttatttc ttattgaaac tattgaaaaa aatcgaagat
      421 aaccaagctt tttccactca aagcttaaac atactaaaaa aaatcgaacg aaccattttg
      481 tttattggtt tcgccttttt tggcttactt cctaaattta tgaaagtagc ggatttagac
      541 gatgctccag gtttgatgtt ggtaggaatc gctgtcgtat tcattccttt tgcagtatac
      601 acattaacaa gcgtgctgga aaaactgctt gaacatgcga ttttacttaa gacagatcat
      661 gatttgacag tgtgaggtgc gacatggcaa ttatcgttaa tttagatgta gtattagctc
      721 aaaaaaaaat gagtgtgact gaattgagtg agaaagtggg cattacgatg gcaaacatct
      781 ctattctaaa aaatggaaaa gcaaaagcca tccgtttttc cactctcgaa aaaatctgcc
      841 aagttttgga gtgtcaacca ggtgatttgt tgacctataa aaatgactcg aatactatac
      901 cagaagaata aaataaagta gaacaaacag taaataaaaa ataatctgaa tgatagatgt
      961 gtgagacttt tagaagttat ttacgtctat tattcagatt ttttatttcg att
//
LOCUS       DACEMO010000350         1013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.362, whole genome shotgun sequence.
ACCESSION   DACEMO010000350 DACEMO010000000
VERSION     DACEMO010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.362"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            334..912
                     /locus_tag="IW728_002786"
     CDS             334..912
                     /locus_tag="IW728_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1026105.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT          352 a          166 c          170 g          325 t
ORIGIN      
        1 tatcatcttc attttactaa ctcctttcta taatattagt ttaccatgaa ggctcacagc
       61 aagacctgta tttagtttgt gaagtaagaa aaaaattgat aattgttgtt attaaaaagt
      121 tgaacatttg tttatatgaa tgatgttttt gaaaaagatc atatgatttg attctcatgt
      181 tttctatttg tacttttctc gtctacttct gttataatct gcatcttact tttttcacct
      241 tttttacttc aacataaaat tataaaagca taatgtttgt gttaatacaa taaagcgttt
      301 acaatgtggt tatcaaatga aggaggtttc tacatgaaaa agattagtac gttattgttt
      361 attgggttag ttagtttagg gttgttcggt tttagcattc caggttttgc gcatggttat
      421 attacttctc caggaagccg tgcataccta ggaacatcag cagcaggaaa cttaaatcaa
      481 aatgtcggaa gagcacaatg ggaaccgcaa agtatcgaag ctaccaaaaa tacattcatt
      541 gatggaaaaa ttgctagtgc aggagtcagc ggctttgagc ctttagatga acagacatct
      601 aacagatggc ataaaaacat ggttaatcca ggaacattaa atattacttg gaatttaaca
      661 gcacaacatc ggacaagtac ttgggattac tacattacca aaccaacttg gaatcctaat
      721 caacctttga aatttagtga ttttgaattg atcacaaaaa tagacgacaa agcaactgtt
      781 ccaccaaaaa cagtcaacca aacgatcaca cttccgcagg atcgaaaagg atataatgtg
      841 attttagcag tatggaatat cagcgatacg acaaatgcat tttatcaagt gatagacgtg
      901 aatatccaat aataatcaaa aagggctgtg acaaaaacta tgtcacaacc ctttttttaa
      961 ataaatagtg tcctcctgtt aactcttcgg tcacaagtac agtattcata aaa
//
LOCUS       DACEMO010000351         1009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.363, whole genome shotgun sequence.
ACCESSION   DACEMO010000351 DACEMO010000000
VERSION     DACEMO010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.363"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            48..>1009
                     /locus_tag="IW728_002787"
     CDS             48..>1009
                     /locus_tag="IW728_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1026106.1"
                     /translation="MESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHE
                     DYAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDC
                     LKAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDG
                     MGNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDL
                     LKGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPY
                     TIERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDR"
BASE COUNT          311 a          178 c          235 g          285 t
ORIGIN      
        1 gtggcgtaac cgcatctata aaaaccatga ggaaaatttt tgaccaactg gaaagcttta
       61 atcccgattt gcctttgacg ttactgtttg actatgtaag gattcgcttt cccacaacgg
      121 atgcacgaaa aattattcaa gaaattctcc atttgaaatt tgagtatatg ctccatgaag
      181 attatgcctt ttactcctat caagaacagt atgtcatggg tgatattgtg gtgatgttgt
      241 ctcatgaaga agataaaggt gtgcttttag aattaaaggg gcgaggttgc cgacaatttg
      301 aaactttttt actcgctcaa aagcgtagct ggtacgactt tttcgaggat tgtctaaagg
      361 ccggtggcgt gatgaaacgc ttggacttgg caatcaatga tcgagtgggg ctattggata
      421 tacctgagct aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt cgtaccttta
      481 aaagttatcg ttccggggaa cttttaaagg ctgatgaaaa ggacggcatg ggcaatacct
      541 tatatatcgg cagtctcaag agtgaggttt atttctgttt gtatgaaaaa gactatgaac
      601 aatatatcaa gttagggatt ccattagacc aaactgagac gaaaaatcgt tttgagattc
      661 gactgaaaaa tgaccgtgct tttcatgcga ttcaagattt gttaaaaggt cgtagcattg
      721 agagcaccac gttttccatt atcaatcgtt acttgcgatt tgcggataaa gtagaaggta
      781 aaaggcgaac caactggccc ttgaatgaac aatgggggcg ttttattgga cggaatcgga
      841 aagagattca attaacttcc gagccaaaac cgtataccat tgaacgaacc ttgaattggc
      901 taggccgaca agtcgcccct acgtggaaaa tggctaagga gttggatcga ttgaatcgga
      961 caacctatat ccaggatatg gtacagaatg cacgcttatc agaccgaca
//
LOCUS       DACEMO010000352         1004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.364, whole genome shotgun sequence.
ACCESSION   DACEMO010000352 DACEMO010000000
VERSION     DACEMO010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.364"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..587)
                     /locus_tag="IW728_002788"
     CDS             complement(<1..587)
                     /locus_tag="IW728_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1026107.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAK"
BASE COUNT          320 a          205 c          187 g          292 t
ORIGIN      
        1 tgttttgctg taaaatacaa aacttgctgc agcaaaaaga tcgttgcaag agaggctgtt
       61 ccgcagatcg ctggatctag atttggcaaa gtcaagactg catcatgtaa attggcgtgg
      121 ttgtccaaaa ccaaatcagc aaattcaaat aatcgcaaac cactgctatg ttgcgatttg
      181 atacttcgtg acaaatcaaa tccagtaaca atgattaatg gatgaccatt ttctttgatc
      241 cactgagcta gttcgatgat tgccgggttt cttccttcat tcgaaagaag aagaaaaatg
      301 tcttctgatt tgacagaaag ctgatcaata attgcctgtc ctgcaccttc tagcgtttca
      361 tagacacctc cggctggatt ttcaatcaat atacttggta caagatccgg ggtttgatcc
      421 caaaattcaa atgctacacc acttagtgtg cggctagcat atatctgcaa tctaccacct
      481 tgattaattt gtttcgcgat ccaagtgcca gcttgaagca tcagcggctc ttctatcctt
      541 aatttctctt ccaattggtg ataatagtca aaataacata aactcattta tctctcccgc
      601 ctagctatac ttgacatgaa tagaaacagc tgcctaatct aaggaagtac ataaaaaaac
      661 agtagttaga cagtaacaca tgtggtcttt gctgtttcaa acacaaaaaa ggcattgaac
      721 gaatacgccc aataccttga catagcaaat aaaacaacgt gcagaccatg gaaaatatct
      781 acataggggt agaattttca ccctagatta ggtacaataa attaggtcga tgcactgcat
      841 tgactatggc aaaatgatcg gtcagggtgt atgtgtgtga ctggtcattc cctgattctt
      901 agcgccaact aagggtcagg gagccttttt ttattcagct gtttctatct gaatttattg
      961 taagtaatct cttatccaag tgcaagcaag aaaatacaaa aaaa
//
LOCUS       DACEMO010000353          998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.365, whole genome shotgun sequence.
ACCESSION   DACEMO010000353 DACEMO010000000
VERSION     DACEMO010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.365"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..124)
                     /locus_tag="IW728_002789"
     CDS             complement(<1..124)
                     /locus_tag="IW728_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1026108.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQR"
     gene            complement(196..720)
                     /gene="infC"
                     /locus_tag="IW728_002790"
     CDS             complement(196..720)
                     /gene="infC"
                     /locus_tag="IW728_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1026109.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
BASE COUNT          245 a          225 c          185 g          343 t
ORIGIN      
        1 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
       61 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
      121 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
      181 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
      241 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
      301 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
      361 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
      421 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
      481 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
      541 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
      601 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
      661 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
      721 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
      781 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
      841 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
      901 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
      961 gaaaatgatt agattagcag actttctagc agtgattc
//
LOCUS       DACEMO010000354          980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.367, whole genome shotgun sequence.
ACCESSION   DACEMO010000354 DACEMO010000000
VERSION     DACEMO010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.367"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..797)
                     /locus_tag="IW728_002791"
     CDS             complement(<1..797)
                     /locus_tag="IW728_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HAQ1026110.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKP"
BASE COUNT          288 a          191 c          170 g          331 t
ORIGIN      
        1 ggtttatcaa tcgccccggc cattaaagtc tgttggccag cctttttata ggcgacaggt
       61 gttccggtac cagcagattt cttaagcgtc agcacaccac tttcttttga attggcattt
      121 ggggtgatta tcaactgatt accattcaca cgataatcaa tgtttgcggc attttccaca
      181 acctcatcga actctgacaa attcagatta ttcgtatccg tcacagtagt cgattgccct
      241 aatactgttt tagccgtact attatggaag gttggtttct tctgataatc gtcaatggct
      301 tgatttattt ttgcctcaat ggctgcgata tttacggcac taccatctga tcgttttatt
      361 gagtgaaggg tatacccatt aacttcttgc cagatcatct tctgtgccac aatatgagta
      421 tctgcatctg tacctacttt tttccataaa acggaaacta atttggcttt atcggacata
      481 tctggtaacg ggtttttctc atagccagga gtgaactcat tgtaaatcgg gactcctggt
      541 tcaatacaga aaatatcttg tttaaagccg ttgtacactg cataatgcgg aacagtgact
      601 tctgacattg gacgaccatc actaaaagtt ccttcaacag catattgatg tgctaaatct
      661 agttctacgg ttaccgtttg tggatgggta atttcatcag caaaagtaat cgtaggagca
      721 attgctccaa caattgcttg aacagccaaa ctcaaaatag ttaatacaca taatttcttt
      781 ttttgttttt tatacatgaa atgttctcct ttcaaattaa gcacaaaaaa ataagatgct
      841 cgcttgaaca tctttgttta taaagtcttt ttaaaatttt tattgactga atagaatgga
      901 tttgagaatg aatgagccta aaaatttaat ctaaaatacg aaatatactg cctttaaatt
      961 agaacacatt ttagaacaca
//
LOCUS       DACEMO010000355          971 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.368, whole genome shotgun sequence.
ACCESSION   DACEMO010000355 DACEMO010000000
VERSION     DACEMO010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.368"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            599..>971
                     /locus_tag="IW728_002792"
     CDS             599..>971
                     /locus_tag="IW728_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1026111.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLK"
BASE COUNT          355 a          174 c          142 g          300 t
ORIGIN      
        1 agcttatacg caaaacacag aaaaaggaat aatctatttc ctacatgaag aaggttacga
       61 tacccaaacc tttatgttta ctgaaaaaaa agaatttcat aaacagatga aaaaactgat
      121 tgaaaaagaa tttccaagaa gtcataaact ctatgtgaat cagcaatttt aacttctaac
      181 tgttctttat taattaaaaa tgttctgtaa taaaataaaa ctttataaca actataacaa
      241 aaccgaatgc aaaacagaag ttctataagt tataatcata gttgttaggt gattcatcta
      301 gtaaaaacac cagatttcaa atttcacaca atttaaacta gatcaatcat caaccttctt
      361 caaattgtta gaaccctttc ccgaggagct gcagcataag caaaaaaaca aacaggaagc
      421 caaatggcta agacattttt tctattagtc tagttgtttc tcgtcgcaag tttaagactt
      481 tagaaagtta tttctaatga tgtttgggtt gttttgatca atgcttatgc acagctcctt
      541 acctattatt tatctcaaca gatctaacaa attaatggac tatttaagga gtttttttat
      601 gcttacgatc tattcttctc gcacacatag ttaccatctg atggcaaact ggctgaaaga
      661 ccatcaaatg ccttacagag aacgaatttt cacacctaaa cagcctttaa caaggaaaga
      721 gatattttat ttattgtcat taacagaaaa cggatttgac gatctattag ctaaacgctc
      781 gattgattat aaaaatttga aagaccaaat cgaccaatgt acgacctcag agcttgtcga
      841 tctgatcgtc tcgcatttta ctttgttaca acgtcccatc attactgacg gaaagaaatt
      901 attagtcgga ttcaatgaag atagcatgcg tctttttatc cctgtacaat ggcgcagaag
      961 tcaactgaaa t
//
LOCUS       DACEMO010000356          966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.369, whole genome shotgun sequence.
ACCESSION   DACEMO010000356 DACEMO010000000
VERSION     DACEMO010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.369"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            17..>966
                     /locus_tag="IW728_002793"
     CDS             17..>966
                     /locus_tag="IW728_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1026112.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTF"
BASE COUNT          351 a          179 c          167 g          269 t
ORIGIN      
        1 aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc tagtcactaa
       61 aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca cactcctacg
      121 gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt ttaattcagg
      181 taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg aattaacatt
      241 ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag cctacaaaag
      301 aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg ttacgatgtc
      361 tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac aaaccatgtc
      421 caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag ccttaaatga
      481 taagtatgtc gctattttta tggacgctac ttacattcca ctaaaacgtc aaaccgtatc
      541 caaagaagcg atttatattg ccattggtat acgagaagac ggcactaaag aagtactgag
      601 ttatgcgatt gctccaactg aatcaacata cgtttggaat gagctgctac aggatattaa
      661 ctccagagga gttcaagaag tcttgctttt tattacggac ggcttaaaag gcatgaaaga
      721 tactatccat caaatttatc ctaaagcaaa atatcagcat tgttgtatcc atgtatctcg
      781 taacatcgct cataaagtac gtgtcaaaga ccgaaaagaa atctgtgatg actttaaggc
      841 tgtttatcaa gctaactcaa aagaagaagc gaataccttc ttatccggca tgattgagaa
      901 atggaagaaa aactatccta aagtgacgca gtcactcata gaaaaccaag acttattaac
      961 ttttta
//
LOCUS       DACEMO010000357          946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.372, whole genome shotgun sequence.
ACCESSION   DACEMO010000357 DACEMO010000000
VERSION     DACEMO010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.372"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..91
                     /locus_tag="IW728_002794"
     CDS             <1..91
                     /locus_tag="IW728_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-aminoethylphosphonate:pyruvate
                     aminotransferase"
                     /protein_id="HAQ1026113.1"
                     /translation="RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            84..212
                     /locus_tag="IW728_002795"
     CDS             84..212
                     /locus_tag="IW728_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1026114.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            216..761
                     /locus_tag="IW728_002796"
     CDS             216..761
                     /locus_tag="IW728_002796"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1026115.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
BASE COUNT          333 a          162 c          202 g          249 t
ORIGIN      
        1 tcgtatcgga aatattggcg agatctatcc ggaagatatt ttggaactga cagcaattat
       61 caaagactat aaggagaacg ttaatgatta aaactgtaat ttttgattgg gcaggaacaa
      121 ctgtggactt tggctgtatg gctcctgtcc acgcttttag aaatgctttt ctagaaaaag
      181 gaacccaact gactgacaaa gaaatccgct aaccgatggg aaaattaaaa tgggatcata
      241 tccaacagct tctttccctc ccttctgtca aaaaacaatg gatagctatt tatggacaac
      301 tacctcagga aaaagaggtg gaggaattgt atcaacaatt tgaacattat ctgtttaaag
      361 atttggtcaa gcattctacc ttgaaacccg acacattgga aacgatcgaa cggttgagaa
      421 acgctgggat caatattggt tccacgaatg gatttaatgc tcctatgatg acaatcgtag
      481 cagaaaccgc aaaaaaagaa aggtattcac cagacttcat ggcaactttt gaagacgtcg
      541 aagggtatgg tcgaccgttt ccttatatga tccataaaaa catgcagtac ttcaaaaaca
      601 agtcggttga tgaagtaatc aaagtcggag atacggtctc agatatccag gaagggaaaa
      661 atgccggagt gttgacagta ggtgtaattg aaggtagttc gctaatgggg atttcttatg
      721 atgagtacca aaaattagag atggggaaaa agatcgttta aaacagtctg ttgctcttag
      781 attccaacaa gccggcgctg atatttgtgt aaacaattta aaagagttaa cagaatacat
      841 tttgtacgct gacaagaatc cagaatgact tttatacaaa ataagaggtt gtgaaaaagc
      901 tgtcctgtat caagtaataa aaacagccaa gcaaaaatag ctcctc
//
LOCUS       DACEMO010000358          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.373, whole genome shotgun sequence.
ACCESSION   DACEMO010000358 DACEMO010000000
VERSION     DACEMO010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.373"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..>943
                     /locus_tag="IW728_002797"
     rRNA            2..>943
                     /locus_tag="IW728_002797"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACEMO010000359          937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.374, whole genome shotgun sequence.
ACCESSION   DACEMO010000359 DACEMO010000000
VERSION     DACEMO010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.374"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..818
                     /locus_tag="IW728_002798"
     CDS             <1..818
                     /locus_tag="IW728_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1026116.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          334 a          182 c          164 g          257 t
ORIGIN      
        1 gtactcgcta tcaacttatt aagcaattga ttcgtacaaa gcaacatttt cttgaaaatc
       61 taacgtacaa atgtaatacg cttgctcgag aaatgcgaga cgaatcgacg agtcttttta
      121 gtgcaaccct catttcactc atgacggaag atttcacatt ggatgaactt gctgagcttc
      181 ctttagaagc tttctgtgat ttacttcaag aaaaaggaaa aggccgtttc aagcaaccag
      241 aaaaaatcgc taaagcaatt caacgtgcaa ttaccatgag ttaccgccta ggtgctcttg
      301 cacaagaatc aattaatgtt gttttaagtg ttctcgtacg agaaatacga gcacttgaaa
      361 aaaatatcaa agaattagat aaagccatcg aacaagtaat tgttgtcatt ccagaatatc
      421 aatgcttaac cagtattcca ggcgttggta aagtctatgc tgccggttta atcgctgaaa
      481 ttggtcaaat cgaaagattt gaagatcaga caaaattggc aaaatacgct ggattaagtt
      541 ggaaagtgaa ccaatctgga aactatcagt cgcaaaatac acctctgaca aaacaaggaa
      601 atcgatattt tagatactac ttagttgaag ctgccaactc tgtaaaaaat tatcttcctg
      661 aatacaaagc tttttatcaa agtaaatata aggaggttcc aaagcatcaa cacaaacgtg
      721 cactcgtcct aaccgcaaga aaatttacgc gtctggtgga tacgctacta cgtaaacacc
      781 aactctatac gccaccaagg agtgtgatag agaaataaca atccgttatt gacgcaaatc
      841 cttgaatcaa cccgaaaaaa atttgatttt gatcgggtct agtttcgtgc gcttcaaaac
      901 atattactca ataaagaaaa tcttaaattt catcttg
//
LOCUS       DACEMO010000360          931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.375, whole genome shotgun sequence.
ACCESSION   DACEMO010000360 DACEMO010000000
VERSION     DACEMO010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.375"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..288
                     /locus_tag="IW728_002799"
     CDS             <1..288
                     /locus_tag="IW728_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026117.1"
                     /translation="LAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPREC
                     WRLQGFPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            313..567
                     /locus_tag="IW728_002800"
     CDS             313..567
                     /locus_tag="IW728_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026118.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
BASE COUNT          289 a          168 c          235 g          239 t
ORIGIN      
        1 ctggcttttc ctaattccac aagtcgtcga ggccgtctgg gaaaacaaag tgtgcatacc
       61 ttattaacag gagaccaaca agcagtcgtc acggatcagt atcaaattcg aaagttaaca
      121 cctagggagt gctggcggct tcagggcttt ccggactggg cttttgatcg agcttctcaa
      181 gtcaattcag atagtcagtt gtataagcag gctggaaatt ctgtaacagt tccggtcatt
      241 tttgatattg ctagaagatt aaaggaggta aaaacagatg atttatgatg agttgattgg
      301 ggaatctact gggtgattgg caagattcag tcagatccgg aattggaaga agaactgtac
      361 cggctcaatt ttgatattcg caaaaacggg gtgaaagtcc ctgatgatcc gtatgtaatt
      421 gatgaggaaa cggatgctcg aatggagctg aatcaagtca tcagtgaatt ggaacgaatt
      481 gcggactttg ctaaagaacc tgatatccgc caatacttgt ttgagataaa agcagagttg
      541 gaaattgagg gtattaccgc ggagtaacgg ccaaataaac cactgatagg cactgaccgc
      601 gggggatttg cgaagcagaa atcctccgtg gtgctgtgcc tgtttatgga cggcgttagc
      661 cggccatgat gtctcacccc ccgtatctaa caggggggta caaaaacaca aaaaataagc
      721 aagcaacgat aaggaatggc tgtcctatca aggataataa gcaatttgtg tagatgttaa
      781 ctgatttgaa aaagttaaca ccttaaattt ggaggtgaga acttggcaca aaggaattta
      841 gattatcggc tattaaaaga ccggcgtaat gaatatggcg tttcacaaaa taaattagct
      901 acgacttgtg gacttagtcg cccgtactta a
//
LOCUS       DACEMO010000361          927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.376, whole genome shotgun sequence.
ACCESSION   DACEMO010000361 DACEMO010000000
VERSION     DACEMO010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.376"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..391
                     /locus_tag="IW728_002801"
     CDS             <1..391
                     /locus_tag="IW728_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1026119.1"
                     /translation="GTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRR
                     GRLGKQSVHTLLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLY
                     KQAGNSVTVPVIFDIARRLKELDVHGL"
     gene            381..671
                     /locus_tag="IW728_002802"
     CDS             381..671
                     /locus_tag="IW728_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026120.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
BASE COUNT          310 a          163 c          222 g          232 t
ORIGIN      
        1 tggaaccgcc cccaccttgt caacgaaaca aggtggtaca aaaatcatga ttcgaaaaaa
       61 tgaaacagaa tatcaagagg tgaagccaga agacagtgtg aatctggctt ttcctaattc
      121 cacaagtcgc cgaggtcgtc tgggaaaaca gagtgttcat accttactaa caggagacca
      181 acaagcgatc gtcacggatc agtatcaaat tcggaagcta acacctagag agtgttggcg
      241 acttcagggc tttcccgatt gggcttttga tcgagattct caagtcaatt cagatagtca
      301 gttgtataag caagccggaa actctgtaac agttccggtc atttttgata ttgccagaag
      361 attaaaagag ttggatgtac atggtttatg atgagttgat tggagaaatt tattgggtga
      421 ttggaaagat tcagtcagac ccagaattag aagaacaatt gcgacggtta aaatttgata
      481 ttcgcaagaa tggtgtcaaa gtcgctggcg atccatattt aatgaatgag gagacggatg
      541 ctcgaattga aataaatcaa gtaattgcag aatttgagcg gattgcggac caagcaaaag
      601 aaccagatat tcgtcaatac ttgttagaaa tgaaagcaga gttggaagta aatggtatta
      661 cagatgagta acctaaacca acacaactga ctgttaggcg ctgaccgcgg aggattcgcg
      721 aagcagaaat cctccgtggt gctgtgcctg ttcatggacg gcgttagccg accatgatgt
      781 ctcacccccg tatctaacag gggggtacaa aaacacaaaa aataagtaag caaccattaa
      841 gaatgcttgt tgtatcaaag atagtaagta atttgtatag gtgttaactc atttgaaaaa
      901 gttaacacct tgaatttgga ggtgaga
//
LOCUS       DACEMO010000362          918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.377, whole genome shotgun sequence.
ACCESSION   DACEMO010000362 DACEMO010000000
VERSION     DACEMO010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.377"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..378
                     /locus_tag="IW728_002803"
     CDS             <1..378
                     /locus_tag="IW728_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ1026121.1"
                     /translation="GQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVRHAS
                     ISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPIEEG
                     ALIIATVDDPKQNVFTLEKVGVI"
     gene            378..>918
                     /locus_tag="IW728_002804"
     CDS             378..>918
                     /locus_tag="IW728_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026122.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQ"
BASE COUNT          310 a          121 c          208 g          279 t
ORIGIN      
        1 ggacaagaat cagattattt gtcatggttt gaatcaatca aggatatttt gaagtctatc
       61 gatccaggtg gagtaatgtt agccgaatta atggatgcac gtgttgatat tcaaggagtt
      121 cgtcatgcgt ctatttcaga gcgcttgctg gcagatatgg attatctata tcagaaatta
      181 cgagcaacac ttttcacgat tgaatacggt gagattgaag tgactgatat tttgcaggat
      241 gatctctttt cagataatca cgaagtcgaa aaagttggaa ctgtagaatt tccaattgaa
      301 gaaggagctt tgattattgc aactgttgat gatccaaaac agaatgtttt cactcttgag
      361 aaagttgggg tgatttgatg gttaaagtaa agagaatgat ggagactgat gaatctggtg
      421 ttaaacgtca atttatgcca attacacatg tttctgcagt tttaggctta gaaaaaatga
      481 tatcaggaca atcaaaagtt ctttcggtta atggtaaata tggtgcagtc attttaacga
      541 aagctgattt aggattagag aatgcaatta ctgaactacc ttatgcaagc gagacaagcg
      601 atggtattct tactgctgaa atgtttcaaa agatcgtaaa tggagaaggt ggaacatata
      661 ttttgccgat tgctactcct gaacaattag gaggtataaa agttggggaa ctcttagaag
      721 ttactgaaga aggtgtttta tctgcaacta agcaaacaga cttcaatttc tctgaagaat
      781 taaagggaaa attagaatct ctaaaaatat taaaagcagg tgcaaatata tcaattgcag
      841 aagatggaac aattagttct acaggtggta gtgggactgg tggtgttaat cagtcatatg
      901 tagatcaaaa attccaag
//
LOCUS       DACEMO010000363          886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.380, whole genome shotgun sequence.
ACCESSION   DACEMO010000363 DACEMO010000000
VERSION     DACEMO010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.380"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..797)
                     /locus_tag="IW728_002805"
     CDS             complement(<1..797)
                     /locus_tag="IW728_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1026123.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKI"
BASE COUNT          265 a          177 c          139 g          305 t
ORIGIN      
        1 ttaattttag caaaagctag tgcatcttga atcaattcga gcacttctgc cttcgactta
       61 tttttagcct gtgcgttctt cgctaattgg gcagcagctc gtgctaagta ataagtcaaa
      121 tggttatccg aacaaatatc gattgcgtaa tttagtagtg catcacttgt ttccaaatca
      181 ccgtcttgcg caaaaaaaac accacaatga aagacgatat ttaacacccg ccagatatct
      241 tctgttgtac gaatcggata cgtataaatt ttttcaaaca ctcgattgaa ataaacttct
      301 gctttttcca actctttttc tctagcatag accattccaa tacctgtata tgccaataaa
      361 cgaaacaacg tgttggctgt ttctgccatc aacaactgat caaaatggaa caacacatcc
      421 gtaattggtg cattcgtaaa aatcatttca aatcctttta gatagtagta acgtgcaatc
      481 agctcagact cgtcaattac cttgggatca atggctgcta aaatctgtgt caattgttca
      541 tattcactcg taatcagtga aaactctgct ttttctagct gttcatcaag ttgtgtattg
      601 atcacgttca tcgaagaaaa aagttcaact aacggaagat tgattcgatt acataatttg
      661 attaaaattt ttaacgaagg gacttgacca ttgttctcaa atttacttaa tgttgcttgt
      721 gtacaaattc cttcagacac ttctttttga gataagttgg cagcctttct tgccgtaata
      781 aatttttcaa tgttcattat tttctctcca atcagacctc acacgaaaat catttctgtc
      841 tcgattttag catgttttct ttgggaatgc tttaattggt taagat
//
LOCUS       DACEMO010000364          886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.381, whole genome shotgun sequence.
ACCESSION   DACEMO010000364 DACEMO010000000
VERSION     DACEMO010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.381"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>886)
                     /locus_tag="IW728_002806"
     CDS             complement(<1..>886)
                     /locus_tag="IW728_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1026124.1"
                     /translation="RQFDISKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQ
                     DHWRSLWHRQKLARMILENHWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDG
                     LLSIRVQISLGKYQDQLLKLEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGID
                     EVVAESGALRLMKNQVWAYDSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDP
                     KNADLADLGTVMPHVYSQKEEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLP
                     PNFLIFDEYVVYMEMLTT"
BASE COUNT          273 a          185 c          149 g          279 t
ORIGIN      
        1 ttcgtcgtta acatttccat ataaaccacg tattcatcaa agattaaaaa gtttggtgga
       61 agtccaagaa acgcataatt ctctcctgtt ttgtagttgg gcatttcttt cattgccttg
      121 ctacgagtca tcatgcgctc atagaaatct tccacacaag cagaaatttc ctccttttgg
      181 gaatagacat gaggcattac cgtacctaaa tcggctaagt ccgcattttt gggatcaaga
      241 acaaaaagct cggcatcaga ttttaataaa gcctcgatga tagtgagtaa aaagtaagtc
      301 ttcccaccac ctgtgccgcc agcgattaac atatgaggta aagaatcata agcccatact
      361 tgatttttca tcaatcgcaa agccccactt tctgccacta cttcgtctat cccaattcga
      421 ttggcaatta tatcatacag taaagtgtac tccacatagg agtctttgag ttccttttct
      481 actagttcac aatatagacc actttctaac ttcttttcca gctttaaaag ctgatcttga
      541 tattttccca gtgaaatttg aacgcgaata gaaagtaaac catccttcat acggtaatag
      601 attttgggga agtaactgat tgtctctttg gttcgactac tattgaggtc tttgaaaaat
      661 ccttcactct gagtttgttt tacttcgtac caatgatttt ctagaatcat ccgggctagc
      721 ttttgtcgat gccacaaact gcgccaatga tcctgaaaaa agtgtatata aagcaacatc
      781 gtgcttcctg aaagaacgag acttcctagc aaatagatta aatcttttat agaacgaacg
      841 atttctatag tagaaagtga tagcttagaa atatcaaatt gtctaa
//
LOCUS       DACEMO010000365          879 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.383, whole genome shotgun sequence.
ACCESSION   DACEMO010000365 DACEMO010000000
VERSION     DACEMO010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.383"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..446
                     /locus_tag="IW728_002807"
     CDS             <1..446
                     /locus_tag="IW728_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1026125.1"
                     /translation="DHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVMALTIAKP
                     ADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFGGVSKKSE
                     SKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            460..>879
                     /locus_tag="IW728_002808"
     CDS             460..>879
                     /locus_tag="IW728_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1026126.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIY"
BASE COUNT          296 a          125 c          174 g          284 t
ORIGIN      
        1 tagaccacag attacttcat ggacaagtaa tattttcttg gactaaacag tatgatatta
       61 accatattat tgtggctgat aacaaagttc caggagatcc aatgtctgtg atggctttga
      121 caattgctaa acctgctgat tgcaaattag acattgttaa aattagtgat gttaaaggga
      181 ttatggaaac atccccaaat gataattaca tgattttaat taaaggacct aaggaagctt
      241 ttgaattagt tcaaaattta ccagaaatta aagaaattaa ttttggcggt gtgtctaaga
      301 aaagcgagtc caaacaatat ggaaaggcag tctttttaac tcctgaagaa ttagaagcca
      361 caaaaaaaat tcttgatcaa gaagtgaaaa tatttgtgca acaagtccca tcttcaagta
      421 ttgaaaatgt agattttagt aagtaagggg ggaaaataaa tgttattaaa agccatttta
      481 ttaggtttag ttggtgtggt agctgttatt gactccaggt taattggtcg acaaaatatt
      541 ggtagaccac taattttgag tacacttgcc ggacttgtgc taggtgactt gcatacaggg
      601 attgttcttg gggcatcttt agaattaatt tctatgggat tcgtctcaat tggggcggct
      661 ggacctccta atatgcaact tggtagtatt attgccactg cttttgcaat tttaactaat
      721 agtagtacag aaacagcctt aacaatagct atcccagtgg ctgtagctgg tgaatttatg
      781 agtattatca tgcgtatgat aattgctcaa tttggacatg ctgcagataa agccattgaa
      841 aataataaat ttagaaaagc tcaaattatt catatttat
//
LOCUS       DACEMO010000366          873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.384, whole genome shotgun sequence.
ACCESSION   DACEMO010000366 DACEMO010000000
VERSION     DACEMO010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.384"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..566
                     /locus_tag="IW728_002809"
     CDS             <1..566
                     /locus_tag="IW728_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026127.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT          304 a          151 c          187 g          231 t
ORIGIN      
        1 aagaacaatt gtatttggaa agtgaaacga gtcaaacgaa ggtcacagtc agccataatc
       61 gtttagacgt gtataaacgt cgtcctgaaa cggtaaaaaa aggaacggag cttctacgac
      121 tagaaacaga aaaatacatc atgaatgttt acctagaaag gagtgagctg gtcgaaaact
      181 ttgtcagcta cacgatttgc aggaaacact attactcaga atcagaatat tattcaattt
      241 ttgagagcat ggaattaatg gaagaagaaa tggaagagct aaacagcaaa gatgcgacga
      301 tagacgatta ttaccttgta tttactagat ttttgacgtg tggaacggaa tctatcgtgt
      361 taatgggaat ggattatgaa ctcgctgtac ccgaagcatt tttgagggaa tatgtagaag
      421 aagtagataa cccaggcgac gactggtaca cgactttaac tgatttttta gacacctata
      481 cttacgacga tacagaagaa atccaaaatg aacttgaccg taaacgaatg ccatatcaac
      541 gatttgaaag gaaaaaaagg caataatcat ttataccgaa aagtgagaat gtgtgaaata
      601 aggacaaaaa acctagtaac taagggaatg gttcttattt cgcaccaatc gctccaaccg
      661 cccccgccgc ttaggtggtg cgaaataagc gggcaacacc cgataaccta agagaaagaa
      721 ggagatccat atgaataaaa tcattcctct attgttagtc ctatttgttg gcgaaatact
      781 tatgcgccat gctgcagctg tgcatttaaa cttcgtagta tttttggcaa tactggtatt
      841 ttgtttgatc ttttggcgct ttgtattagc cct
//
LOCUS       DACEMO010000367          864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.385, whole genome shotgun sequence.
ACCESSION   DACEMO010000367 DACEMO010000000
VERSION     DACEMO010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.385"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..>864
                     /gene="gmd"
                     /locus_tag="IW728_002810"
     CDS             82..>864
                     /gene="gmd"
                     /locus_tag="IW728_002810"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAQ1026128.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAA"
BASE COUNT          207 a          205 c          209 g          243 t
ORIGIN      
        1 tatcgtgtat tcttgaaaag tattagaaat tacgagccat ttatatggtc ttatcactta
       61 cataggaaag gataatgtat catgaaaaaa gcgctgatta ctggtattac tggtcaggac
      121 ggttcctatc tggcagattt cctgctagag aagggctacg aagttcacgg tatcactcgc
      181 cgtgcttcta tttccaatac cgctcgcatt gaccatttga tgggcaagat cacccttcat
      241 gacggtgacc tgtccgactc atcttctctg attcgtatca tcagcattgt tcagcctgat
      301 gaaatctata acctcgctgc acagagccat gttcaggttt cttttgatgt tcctgagtac
      361 tccggcgatg ttgatgcttt gggcgttatg cgtattctgg aagcttgccg tattctgggc
      421 ctgacgaaga agactaagat ttatcaggca tctacttctg agctgtatgg caaggttgaa
      481 gaagttcctc agcgtgaaac cactccgttt catccttaca gcccttatgc tgttgcaaag
      541 cagtatggct tctggatcac taaggaatat cgtgaagctt atggcatgtt cgctgtcaac
      601 ggtattctgt tcaaccatga atccgaacgt cgcggtgaga atttcgtcac ccgtaagatc
      661 accctcgctg ccggccgcat tgcagaaggc ctgcaggacc atctggaact gggcaacatg
      721 gactccctgc gtgactgggg ctatgcaaag gactatgttg agtgcatgtg gatgattctc
      781 cagcatgaga ctcccgagga cttcgtgatc gctaccggcg agcagcacac tgttcgtgac
      841 ttcaccgaga aggcattcgc agca
//
LOCUS       DACEMO010000368          861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.386, whole genome shotgun sequence.
ACCESSION   DACEMO010000368 DACEMO010000000
VERSION     DACEMO010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.386"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..120
                     /locus_tag="IW728_002811"
     CDS             <1..120
                     /locus_tag="IW728_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1026129.1"
                     /translation="TTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            486..746
                     /locus_tag="IW728_002812"
     CDS             486..746
                     /locus_tag="IW728_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026130.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
BASE COUNT          286 a          153 c          160 g          262 t
ORIGIN      
        1 accaccagtc caagccccac tacctttgaa caacgggata caaacaatgg attggacgct
       61 tcacaaaatc catttggtgg acaatcaatc gatattagcg atgatgatct accattctaa
      121 gtagcatttt ctattggttg catttgctac ggtttatgtt ataatttagt caacaaaaag
      181 aattttcaat ctataaccaa aaaccactag ctggcgggct agtggttttc ttttacgttt
      241 ctattataag aattaacggg taggcttact gtctttcttg gcaaatttac aatcaagcca
      301 tttgcagata tagtgcgcgg ctacttctgc catgattgca aaaacaaaat caaaaagaat
      361 tttcaaacct ataacctcct ccccttgcca gattaggccg gggtagcaga cagcaagact
      421 attatagcat aattgaaata ttcaaatgat tggatttttg ctataatttt agcgaggtga
      481 ttgtgatgaa caaatatgtt ggtttgcttg ataaaatccg agtaataaaa acgcaaccgt
      541 tattggttcg ctttacttta caaacgattc atgaatcgat taattgtgta gttgcagata
      601 ttgaaattat cgataaattg ctaattatgg atgatggaaa atacaatatt gccgtcacag
      661 gtcacttcaa taaacgaaac cagctagtta ttgctagcat gtatgttaga aatcccgatc
      721 agtttacgcg attgatgggc atataaaaaa agaagagttg caatcctaat gtaggactgc
      781 agctcttttt gttttacata gaaacaagaa aaatgaaaag gcctttttag gaaggcgcga
      841 gcgccttctc aattgaaaaa c
//
LOCUS       DACEMO010000369          844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.389, whole genome shotgun sequence.
ACCESSION   DACEMO010000369 DACEMO010000000
VERSION     DACEMO010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.389"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>844
                     /locus_tag="IW728_002813"
     CDS             <1..>844
                     /locus_tag="IW728_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1026131.1"
                     /translation="DMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFIST
                     FLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYA
                     LKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAW
                     VPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVV
                     PITLGYFFQFLYSLPVNAEFYEKKTSFITIGTVASAIVNILLNMIFIPRLGIIAAGYT
                     TVFTY"
BASE COUNT          262 a          106 c          147 g          329 t
ORIGIN      
        1 gatatgaacc aacttgtatt taatttgatg ttattacaaa gttttgctag ttttgtaatt
       61 agttatatcc agtcttattt tacgattaac tttaaccatt ttaaatattt gattttatct
      121 tttatatcta cttttttgaa tattgcaatc tctgtagctt tgatgcagac attatatttt
      181 gatgataaat atgttggcag agtagtcggc ggaacactgt catttgtgtt gataggttta
      241 gtaatttatt taagaataat gataaagggt gctcattttt ttgataaaaa acagtggaga
      301 tacgctttaa aaatctcttt gccgctaatc ccacattcct tagcaaatat agttttagca
      361 caattcgata gaattatgat taattcttat ttaggtcctg ctgcgggggg aatatattcc
      421 tacattagta acataggtat tattctaagc gtaatatgga tgtctactaa taacgcctgg
      481 gttccatggt tctatggtga gatgagtaaa aataatgaaa aaaaagtgaa gaaagttagt
      541 aactattatt tagtagtctt tactttgatt acgatgattg taatggtagt ttcgattgat
      601 ttagcaaaat ttatggctcc gaaagactat tattctgggt tatctttagt ggttccgata
      661 accttaggct atttctttca gtttttatat agtttaccag ttaatgctga attttatgag
      721 aaaaaaacat cgtttattac gattggtaca gtagcaagtg ctatagttaa tatactatta
      781 aacatgattt tcatacctag gttaggaata attgctgctg gatacacaac cgtatttacg
      841 tatt
//
LOCUS       DACEMO010000370          841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.390, whole genome shotgun sequence.
ACCESSION   DACEMO010000370 DACEMO010000000
VERSION     DACEMO010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.390"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>841
                     /locus_tag="IW728_002814"
     CDS             <1..>841
                     /locus_tag="IW728_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ1026132.1"
                     /translation="KTVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGEAVTVDL
                     QVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLK
                     QKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQATFL
                     ANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQ
                     NVAACTSEACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLK
                     EDFT"
BASE COUNT          328 a          148 c          194 g          171 t
ORIGIN      
        1 taaagacagt cggaaaagta ccagtagatc taggttggaa aaagctgttt ggcaaagaga
       61 gcgacagaaa agaaaaggaa gaagaaccgc tacttcctaa agtgacaaaa ggagaagcgg
      121 tgacggtaga tctacaagtc cttgaaaaag aaacgaagcc accacaacct tataccgaag
      181 gtacgttgat cacagcgatg aaaacagctg gaaaaaccgt ggatagcgaa gaagcacaat
      241 ccatattgaa agaagtagaa ggaatcggta cagaagcaac acgagcaaat attattgaaa
      301 ctttgaaaca aaaggaatat atcaaggtag aaaaaaataa gcttgtggtg acaaataaag
      361 ggattttact ttgccaagcc gtggaaaaag agccgctgct aacaagtgcc gaaatgaccg
      421 ccaaatggga aagttactta ttaaagattg gtgagcagaa aggaacacaa gctactttct
      481 tagctaatat ccaaaaattt gtctctcact tgttggaagt cgtaccggga caaatccagt
      541 caacagattt tggatcaacg ttacaagagg tgaaagcagc cagtgaaaaa caggaagcaa
      601 cgcgtcattt aggtgtttgt ccaaaatgtc gggaacaaga agtcttgctt tatcaaaatg
      661 tggcggcatg tacgtctgaa gcctgtgatt ttaaattatg gacaacgatt gccaaaaaga
      721 aactcacagc cacacaactg aaagagatca ttcaaaacgg ccgaacaagc caatcagtca
      781 gaggactaaa aggtcagaaa ggttcgtttg aagcaacgat cgttttaaaa gaagacttta
      841 c
//
LOCUS       DACEMO010000371          839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.391, whole genome shotgun sequence.
ACCESSION   DACEMO010000371 DACEMO010000000
VERSION     DACEMO010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.391"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            17..835
                     /gene="purR"
                     /locus_tag="IW728_002815"
     CDS             17..835
                     /gene="purR"
                     /locus_tag="IW728_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1026133.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
BASE COUNT          268 a          146 c          191 g          234 t
ORIGIN      
        1 aaaggagttg gaataggtga aagtacgtcg cagtgaacgt ctagttgaca tgacacagta
       61 tttattgaat cacccgcatg aattgatttc cttaacaagc tttgccgaac gttatgaatc
      121 tgctaaatct tctatcagtg aagatttggg aatcatcaag aaaacattta aagaacgagg
      181 gtatggaacc ttggaaacaa tcccgggtgc tgcaggtggt gtattgttca tccctgaaat
      241 ttcatatgaa gaggcgaaaa aatatattga atctctagca gaacgtctat cagaacaaga
      301 ccgtctgctt ccaggaggat atgtttatct ctctgatttg ttaggagaac ctgaactgct
      361 gcgtcaagtt ggaagaatca ttgcttctaa ataccttggt gaaaaaatcg atgctgtaat
      421 gactgtagcc acaaaaggcg tacctattgc ccaagctgtg tcctattatc tgaatgcgcc
      481 attcgttatt gtacgccggg actcaaaaat aacagaagga tcaacagtaa gtgtcaacta
      541 tgtgtctgga tcttctgaaa gaattgaaaa gatggaactg tctaagagaa gtttgaaacg
      601 tggttcacgt gtccttgtag tggatgattt catgaaaggc ggcggaacag tcaacggaat
      661 gaagagcttg attgaagaat tcgaatcaga acttgttggc atcactgtat ttgccgaatc
      721 aaaattcaac ggtcatcgcg ccatcgatga ttatacgtca ctgctttatg tcaaagatgt
      781 agatactcaa acgaaaaata ttacggttgt gccaggaaat tatttttcag aataagagg
//
LOCUS       DACEMO010000372          831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.392, whole genome shotgun sequence.
ACCESSION   DACEMO010000372 DACEMO010000000
VERSION     DACEMO010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.392"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            32..>831
                     /locus_tag="IW728_002816"
     CDS             32..>831
                     /locus_tag="IW728_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1026134.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFP"
BASE COUNT          287 a          140 c          168 g          236 t
ORIGIN      
        1 gaacccagta ctcttgtaag gagtgtgaac tatgtatccg attaagtata ttgaaaataa
       61 tctcgtcttt aaccaagaag gcgagtgttt tgcctactat gaattagtgc cttacaatta
      121 ctcctttttg tcacccgaac agaaatttca agtgcatgat aactttcgcc aactaattgc
      181 gcaaaatcgt gaaggcaaga tccatgcgtt acaaattgcc acagaaagta gtattcgcgc
      241 cacacaagaa cgatcaaaaa aagaaattac cggtagactc aaagaagtag cgaaacaacg
      301 aattgatata cagacagatg cattagtatc catgattggc gatagtcaga ttgattatcg
      361 cttttttatt ggctttaaac taattgcgac agatgaagaa gtcaatctaa agagtctcaa
      421 gaaatctttc ttttctgggc ttcaagaatt tgtctatgga gtgaatcatc atttgatggg
      481 cgattttgtt tccttatcca atgaagaaat tcgctgctat tctaagcttg aaaaattaat
      541 ggaaagcaaa ttggctcgaa gatttaaagt caggcgagtc acaccgagtg acctgacgta
      601 tttgattgaa catatttatg gcgaaaaagg gataccgttt gaagaatacg aatttcagct
      661 accaaagaaa aagctaaaat cagaaacact ggtgaaacgt tatgacttat tacgtccaaa
      721 tcgttgttta attgaagaaa aaccaaggtg cttacggatg gaacatgaga atcatgagtc
      781 gtacgtagcg tatttaacaa ttaatacaat tgtgggggag atggaatttc c
//
LOCUS       DACEMO010000373          823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.394, whole genome shotgun sequence.
ACCESSION   DACEMO010000373 DACEMO010000000
VERSION     DACEMO010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.394"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..512
                     /locus_tag="IW728_002817"
     CDS             <1..512
                     /locus_tag="IW728_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1026135.1"
                     /translation="IDYLAELYDDDYSKDTHFSNLLNEIKENYDFIIIDVPPQLNKFT
                     NSALVASDYVIVILQTQERSLKGAEKYIEHLIQLNNDYGTEIDILGLLPVLVQNGNDL
                     DLDIIEDAESLFGKSNIFNTKIKTMQRLKRYDRTGITDNPKDIHDKRVQQVYQSVSDE
                     VIQRINLLG"
     gene            526..>823
                     /locus_tag="IW728_002818"
     CDS             526..>823
                     /locus_tag="IW728_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026136.1"
                     /translation="MTNEKNESIGLNAIKRKNKTQRPENIVPENSEALNNTTDSGKIL
                     EQSKKRLTTKDLPKSIRMPLDTHTAISTIATIEDKKIYETLNKIVEFYINNMNPA"
BASE COUNT          353 a          120 c          120 g          230 t
ORIGIN      
        1 atattgatta tttagcggaa ttatatgacg atgattattc taaagatacg catttctcaa
       61 atttattaaa tgaaattaaa gagaactatg attttattat cattgacgta ccaccacaat
      121 taaataaatt tactaattct gctttagtcg caagtgacta tgttatcgta attctgcaaa
      181 cacaggaacg ctcgttaaaa ggagctgaaa aatatataga acatttaatt caattaaaca
      241 atgattatgg aacagaaata gatattttag gcttgctacc ggtactagtt caaaacggta
      301 atgatttaga cctcgatatt attgaagacg cagagtcatt atttggaaaa tcgaatattt
      361 tcaatacaaa aatcaaaaca atgcagagat tgaaaaggta tgatagaact ggcataaccg
      421 ataacccaaa agacattcat gataaaagag tccaacaagt ttatcaaagt gttagtgacg
      481 aagtaataca acgtattaat cttttaggat aaggagcttt taaaaatgac taatgaaaaa
      541 aatgaatcaa ttggtttaaa tgccataaaa agaaaaaata aaacacaacg acctgaaaat
      601 attgttccag aaaattctga agccttgaat aatacaactg attcaggcaa aattctagaa
      661 caatccaaaa aacgactcac aactaaagat ttacctaaat ctataagaat gccactagat
      721 actcacactg caatcagcac gattgcgaca attgaagata aaaaaatata tgaaacgtta
      781 aataaaattg tagaatttta tataaacaac atgaatccag caa
//
LOCUS       DACEMO010000374          798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.395, whole genome shotgun sequence.
ACCESSION   DACEMO010000374 DACEMO010000000
VERSION     DACEMO010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.395"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..408
                     /locus_tag="IW728_002819"
     CDS             <1..408
                     /locus_tag="IW728_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1026137.1"
                     /translation="SLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYR
                     QFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTD
                     HRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            484..702
                     /locus_tag="IW728_002820"
     CDS             484..702
                     /locus_tag="IW728_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026138.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
BASE COUNT          318 a           99 c          142 g          239 t
ORIGIN      
        1 tcactttcaa ctaaaaataa taaattagat gaagaattga tcgaaacatt tattgaagaa
       61 aatcttctta aacaaatatg gggagaaagt attgtgaatt gcttaaaact ggctagcaat
      121 agtgattatc gtcaattcga caactggtac aaaaaattca aatatgcgat tagatctgct
      181 gaaaaagaac aaaaaattca actgaaaatt atctatgaaa tttgtaataa caagtatttt
      241 gtagatcatg ttagagaaca gctatcaatg actttacgtg acttgatacg aagagcaaaa
      301 acagatcatc gtatcaaaca aaaagacgat tatatctttg cttctctaaa aaataaagcg
      361 ttagaactaa ttgaattgca aatcagcgaa ggaatagata agcaataaga atagaattga
      421 gtcaaatgaa atggaggggt atctccactt catttgagaa ctttgaatat aggaggggtt
      481 attttgatta aaaatcgttt aatagatcaa gtttggtgtc tagatcaaaa tacagatagt
      541 ctcgaattaa tcgagactat ttgcttcaac actatagttc tcgatttaag agctgaaaat
      601 gataattgtg taactcatgt cataattaat caaaaaatgg ctcagaagct ctctgagagc
      661 ctcagaaaat gggctaaggg aggtaacgat gaaaataatt gaaaaaaaga ttgccaataa
      721 aaaaataaat tggttggttg ttaattttct tgttctgata gctatgatta tttttacaag
      781 taatattgct catgcgag
//
LOCUS       DACEMO010000375          794 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.396, whole genome shotgun sequence.
ACCESSION   DACEMO010000375 DACEMO010000000
VERSION     DACEMO010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.396"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..558
                     /locus_tag="IW728_002821"
     CDS             <1..558
                     /locus_tag="IW728_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1026139.1"
                     /translation="DTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENEL
                     AVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYF
                     SVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSR
                     VFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            575..>794
                     /locus_tag="IW728_002822"
     CDS             575..>794
                     /locus_tag="IW728_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026140.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGS"
BASE COUNT          287 a           91 c          146 g          270 t
ORIGIN      
        1 gatacgattc ggtctgaaaa aaattattct atcattgttt ttgaaaagtt agcacaaacg
       61 tctataacat taggaatgga tataatagaa gcgtatcaaa gtagagatgc tttgatacaa
      121 gaaaacgaat tagctgtatc gttacctgag gtcttaaaag tgagggattc tgggattgtt
      181 tattatacta aggaaatcgg aaaaactaag atagagcatc tttctccctt aatttcttct
      241 gtcgttcaat tcatcggctt aaatatctat aaaagaatta ctgtaaagga aatagctaat
      301 tatttttctg taagtgaaac taaattaaga gaaagcttta agaatgaaat gcatattacg
      361 atttataatt atatctctaa gcgaaaaatt tctacggcta aaataatgtt gaaatcaaac
      421 catactatca gtgaagtttc tttaggttta ggattttcag attcttcaca cttttcgaga
      481 gtattcaaaa agtacgcagg tgtatctcct aagcaatatc aattaggttt agtggacaat
      541 ttcaacaaca ttagctgaga ggttttattt tattatgaga ctatatcatt tacgtagttt
      601 ggaggagttg aggaaattgg attttaacaa aaaatttttt tgggaaattt ctgatttgag
      661 ttgtttcgta gaatctgagc gtctaagtaa tggtaataat agtgtgttaa atgaatatga
      721 caaagtagta attaataaag taaaaagttt atgtgaagaa gctagagaat ctatcagtca
      781 tattaatggt agca
//
LOCUS       DACEMO010000376          781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.397, whole genome shotgun sequence.
ACCESSION   DACEMO010000376 DACEMO010000000
VERSION     DACEMO010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.397"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            170..781
                     /locus_tag="IW728_002823"
                     /pseudo
     CDS             170..781
                     /locus_tag="IW728_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          313 a          126 c          151 g          191 t
ORIGIN      
        1 ggcgtcatta tgatttctaa gagaaaatgg gagttgttac aagaagaact ttatttacaa
       61 cgaacgggta catgggctta tgtatctgat ttaatggata ggctacaatt aactagacag
      121 aaaaattaag gtgtgtagac tagaagaaaa cataccagga ggaattttta tgtctaagag
      181 aacacgaaga actttttcac aagaattcaa gcaacaaatc gtcaatcttt acttagctgg
      241 aaagccacgt gtagaaatca ttcgagaata tgaactaacg gcttcagcat ttgacaaatg
      301 ggtaaagcaa tctaaaacga gtggttcatt caaagaaaaa gataatctta cgcctgaaca
      361 aaaagaattg ttagaactac gtaaaagaaa ccagcaatta gaaatggaaa atgatatttt
      421 aaagcaagca gcgctgatat tcggacgaag agacaagtaa tcgatgcgaa taagcatctt
      481 taccctatat cagcgatgtg cagaatatta ggtctatcac gtcagtccta ttattatcaa
      541 tcaaaaccaa agaaagacga atcagaactt gaagaagtag tcgctgaaga atttatccgc
      601 agccgaaagg cctacggctc aagaaaaata aaaaaagcct tatcaaaacg aggcattcag
      661 atcagccgac gaaaaattag tagaatcatg aaaaatagag gattaaaatc gagctatact
      721 gttgcttatt ttaaagtaca tcattctact tgcaatgaag ccaaaacgac aaacgtattg
      781 a
//
LOCUS       DACEMO010000377          770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.399, whole genome shotgun sequence.
ACCESSION   DACEMO010000377 DACEMO010000000
VERSION     DACEMO010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.399"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..390)
                     /locus_tag="IW728_002824"
     CDS             complement(<1..390)
                     /locus_tag="IW728_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1026141.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTS"
BASE COUNT          262 a          139 c          143 g          226 t
ORIGIN      
        1 ggaagttgtg aatcgttgaa tacgcaagaa atcagcgatt cttaatgcat cgtttcgatc
       61 agttttattc tcatcaaaca tgcgactaaa ttgtttcacc cgaaaaggat tttcaatcgt
      121 tacaagtgtg ttgagcttct ttagtttctc atcttcatga aaaaacatgg aaggatggaa
      181 gctgtacatg gacgttgact ccatgccaat cacaatttga tcaaagtggt agctgtcatt
      241 gaattcaaga attgtttctc gtatgaatga agcaccttcg atgtcattgg caacagagat
      301 ttcagataag atagacaatt gattgtcgtc tgttaaaaaa caaacatcta gtttttcaga
      361 gctaacgtca ataccgacaa ataatttcat ggaagaagga cctcctttcg atataaattt
      421 tggaaaatgc ttcgatactg tgggatttcc cagaaactca ctgtatagcc acaacctcgc
      481 ctaatagaat acggtgataa aagagctaga agctgccttc caaactacta tttgaaagtc
      541 tagtccatta tcgaaacagc taatgagttg gtagtgataa cagtggttcg ggtaacagac
      601 taaacagggt agacaaacgc tacaggagga aatagcaaat tcccgttgga tcctttccat
      661 acatccattg tactctgaga aatcacacaa tatcgaaaca aattccgtta ggaatgtgtt
      721 attcaataaa ttttataatc tagaaacatt ttgaataaaa actattagac
//
LOCUS       DACEMO010000378          747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.406, whole genome shotgun sequence.
ACCESSION   DACEMO010000378 DACEMO010000000
VERSION     DACEMO010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.406"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            553..>747
                     /locus_tag="IW728_002825"
     CDS             553..>747
                     /locus_tag="IW728_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1026142.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
BASE COUNT          271 a          136 c          114 g          226 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaactgta
       61 tacataaaaa aagtcaagta ttagaaaaaa gttcaaaaga tcaagtgtgc gcttcgctcc
      121 cactcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
      181 agttttcggg atacattctc cttctcttcc taataatcaa ttaaaatttt aatctttata
      241 tttctgctaa tcactcagga aacagtacag aaaaacaaac acgtatcata gatataaatg
      301 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
      361 tgtccgtttt tgggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgttcta
      421 agtcaaaaaa ctctgacagc gcctttatat agataaagtc cgtataattg tgtaaaagta
      481 aaaaggccat ataacagtcc ttttacggta caatgttttt aacgacaaaa acatacccag
      541 gaggactttt acatgaccca agtacatttt acactgaaaa gcgaagagat tcaaagcatt
      601 attgaatatt ctgtaaagga tgacgtttct aaaaatattt taacaacggt atttaatcaa
      661 ctaatggaaa atcaacgaac agaatatatt caagcaaaag aatatgaacg aacagaaaac
      721 cgacaaagtc aacgaaatgg ctattat
//
LOCUS       DACEMO010000379          746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.407, whole genome shotgun sequence.
ACCESSION   DACEMO010000379 DACEMO010000000
VERSION     DACEMO010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.407"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..716
                     /locus_tag="IW728_002826"
     CDS             <1..716
                     /locus_tag="IW728_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ1026143.1"
                     /translation="KAIEKVPLDLGWKKLFGKESDGKEKEEEPLLPKVTKGEMVTVDL
                     QVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLK
                     QKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGERKGTQTTFL
                     ANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYQ
                     KVAACTSEACDFKRSEGFI"
BASE COUNT          274 a          133 c          184 g          155 t
ORIGIN      
        1 taaaggcaat cgaaaaggtg ccgctagatc taggttggaa gaagttattt ggaaaagaga
       61 gcgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg
      121 tgacggtgga tctacaagtt cttgaaaaag aaaccaagcc accacaacct tataccgaag
      181 gtacgttgat cactgcaatg aaaacagctg ggaaaaccgt ggatagtgaa gaagcgcaat
      241 ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa
      301 ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag
      361 ggattttgct ttgccaagcc gtggaaaaag agccgctgct tacgagcgcc gaaatgaccg
      421 ccaaatggga aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc
      481 tagccaatat ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat
      541 ccactgattt tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg
      601 cgcgtcattt aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg
      661 tggcagcttg tacgtctgaa gcctgtgatt ttaaaaggtc agaagggttc atttgaagca
      721 acgattgttt taaaagaaga cttcac
//
LOCUS       DACEMO010000380          737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.408, whole genome shotgun sequence.
ACCESSION   DACEMO010000380 DACEMO010000000
VERSION     DACEMO010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.408"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>737
                     /locus_tag="IW728_002827"
     CDS             <1..>737
                     /locus_tag="IW728_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1026144.1"
                     /translation="TRSQRRKKLAQMIQENIKEELGLIATVGVGDNPLLAKLALDNEA
                     KHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMSIRDLANWNPYTI
                     KNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEM
                     AEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDK
                     YYEGQEVRHVGITYSKLVYTDSLQLDL"
BASE COUNT          262 a          121 c          168 g          186 t
ORIGIN      
        1 aacacgaagt caacggagaa agaagctggc tcaaatgatc caggagaata ttaaagaaga
       61 gctaggattg attgctacag taggtgtcgg agataatccc cttttggcta aactagcctt
      121 agacaacgaa gcaaagcata atgaaggatt tgttgcggaa tggacgtacg aaaacgttcc
      181 tgaaaaagta tggaatattc ctgaaatgac ggacttttgg ggaattggct ctcgaatgaa
      241 aaaaagactc aatcagatgg ggattatgag cattagagat cttgctaact ggaatcctta
      301 tacgattaag aatagactag gtgtgattgg cttacagctg tacttccatg ctaacggaat
      361 cgatcgaaca gatatagcaa tcccgccaga accgacaaag gaaaaatctt atggtaattc
      421 acaagtgtta ccacgagatt atactagaag aaatgaaatc gagctggtgg taaaagaaat
      481 ggcggaacaa gtagccattc gtatcaggca gcataattgt aaaacaggat gtgttcattt
      541 aaacattggt acgtcgatct tagaaacgag accaggattt tcacaccaga tgaaaatacc
      601 tatcacagat aatacaaagg aattgcagaa ttattgtctt ttcttgtttg ataagtatta
      661 tgaaggacaa gaagtaaggc acgttggaat aacctatagc aagcttgtgt acaccgattc
      721 gcttcagctg gatttat
//
LOCUS       DACEMO010000381          702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.411, whole genome shotgun sequence.
ACCESSION   DACEMO010000381 DACEMO010000000
VERSION     DACEMO010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.411"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          266 a           88 c          133 g          215 t
ORIGIN      
        1 accaaatcta gccgttataa cgaagtgttt aaggaaaatg tgaagttaga aagtgactta
       61 aacaagctaa aaaaataaaa agaagaaaac ataagttaac taaaattatt ttattaagaa
      121 tgatggaata taaggataag gttaccaaca taacgatcat tacttctctg atatgctatg
      181 gacttctatc aatcagtatt ttgtctatag attatggagt aactatactg caagccttag
      241 ttttggctat gcctatgtat ctatctttgt ttatcatgca tgaaatatat aagctatttg
      301 ttttatcaaa aaatgtagaa atacgggtaa atattttggt gaatagttat caaaaagaac
      361 tcaaagaaag aagacggtgg cggtatttat tgaaaggatg aaaaagtagg atttgacata
      421 ctggtattca gaaagtgccg tcacttttaa aacaatggaa aaggtggaga taagatgaag
      481 aaaaaacaaa ctaaactatc attagcagat atttaacaca gttaaccgca actgaagatg
      541 gtgttgttga atttgaacaa tctgaaattg cggtagtaga tgatcgttac cttaaaatgc
      601 cttacttctt tgaccaagca aaggtcattt gcctttgtca ctacgatggg gtacgtgatt
      661 ttaggcgtta aaaagtagtg attatatcaa ctttacgagt aa
//
LOCUS       DACEMO010000382          702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.412, whole genome shotgun sequence.
ACCESSION   DACEMO010000382 DACEMO010000000
VERSION     DACEMO010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.412"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW728_002828"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW728_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1026145.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="IW728_002829"
     CDS             complement(481..675)
                     /locus_tag="IW728_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026146.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          213 a          153 c          115 g          221 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ct
//
LOCUS       DACEMO010000383          666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.418, whole genome shotgun sequence.
ACCESSION   DACEMO010000383 DACEMO010000000
VERSION     DACEMO010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.418"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..574
                     /locus_tag="IW728_002830"
     CDS             <1..574
                     /locus_tag="IW728_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAQ1026147.1"
                     /translation="DYERVRAPIVAFDRNLAPNVSIVSSDNFEGGKLAAKTLQKNGCQ
                     NTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRREMEIKSTIINHKPDG
                     IFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQLTTIRQPIDEIASL
                     IVDILLKKIKGEKTNKDYILPISILSGGSI"
BASE COUNT          244 a          100 c           98 g          224 t
ORIGIN      
        1 tgattatgag cgtgtgagag cacctattgt tgcttttgat agaaatttag ctcctaatgt
       61 ttccattgtt tcttcagata actttgaagg gggaaaactt gcagctaaga ctttacaaaa
      121 aaatggttgt caaaatacca ttatgattac aggaaatgat aacacggatt ctcccacaga
      181 attacgtgca ctaggttttt cttttcaaaa tccaaatgga aaaattttca aaattcctaa
      241 taatctgtct actatccgtc gtgaaatgga aataaagtca actattataa atcacaaacc
      301 agatggtatt tttgtttctg atgatttaac agccattctc actatgaaaa ttgctcatca
      361 acttaaactt aatatcccag aagatttaaa aattattgga tacgatggta cctcttttat
      421 tgagaaattt ttcccacaat tgacaacaat aagacaacca attgatgaaa ttgcaagtct
      481 tattgttgat attcttctga aaaaaattaa aggcgaaaag acaaataagg attatatttt
      541 accaatctca atactttctg gaggaagtat ttaacgttaa attaattttt taatgtctat
      601 aaaattcgtt agaaaataca atttgtagat atccaattga tttgtaaaca aaaaggagtt
      661 gtcaac
//
LOCUS       DACEMO010000384          638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.423, whole genome shotgun sequence.
ACCESSION   DACEMO010000384 DACEMO010000000
VERSION     DACEMO010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.423"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>638
                     /locus_tag="IW728_002831"
     CDS             <1..>638
                     /locus_tag="IW728_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026148.1"
                     /translation="DGVAWSLIKQGVTDLAYINKTDQYSSIDKTEDRNNLTRKNFQQT
                     DLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDNFLSTFSDSLKSGYY
                     RYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLVFATDIETGQRSKVV
                     LSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIAYICAVFVL"
BASE COUNT          212 a           95 c          119 g          212 t
ORIGIN      
        1 gacggagtcg cttggtcatt gataaaacaa ggagtaacag atttagcata tattaataaa
       61 acagatcagt actcaagcat tgataaaacg gaggatcgaa ataatttaac acgaaaaaat
      121 tttcaacaga cggatctcac acaagtgtta acggataaag tgattgataa attggaaaaa
      181 gaaaatccaa ttgcggacaa tcttcgttat gaattagttg aaaattctaa taatgaattt
      241 gttgctacaa agttttctga caacttctta tcaacatttt ctgatagctt gaaatcaggc
      301 tattaccgat accaagcaaa tctatggggg atttcaattg ggttgacagc gttagcaatc
      361 gcctatgtgt tcagcgcgtt tgtgatcatt acagcaatac tagaacttgc atttaaacgt
      421 gtgttaggtg ttcttgtttt tgcaacagat attgagacag gacagcgttc aaaagttgtt
      481 ctatcagata ttctacaatg ttacttaact gttgggtttc aaggatttgg gttatcaatg
      541 tttgctatgt ttatcaattt tctgaactca ggacaaggta tctcaacaaa tatttttata
      601 aaaacaattg cttatatctg tgccgttttt gttttaat
//
LOCUS       DACEMO010000385          630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.426, whole genome shotgun sequence.
ACCESSION   DACEMO010000385 DACEMO010000000
VERSION     DACEMO010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.426"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>630
                     /locus_tag="IW728_002832"
     rRNA            <1..>630
                     /locus_tag="IW728_002832"
                     /product="16S ribosomal RNA"
BASE COUNT          156 a          144 c          196 g          134 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
      481 ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat tcagttgggc
      541 actctagcaa gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat
      601 gccccttatg acctgggcta cacacgtgct
//
LOCUS       DACEMO010000386          627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.427, whole genome shotgun sequence.
ACCESSION   DACEMO010000386 DACEMO010000000
VERSION     DACEMO010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.427"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>627
                     /locus_tag="IW728_002833"
     CDS             <1..>627
                     /locus_tag="IW728_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1026149.1"
                     /translation="WFRNLRHYLNTQEILEKHNVSWNAVSQQYYDTTTAYGRTFIAQV
                     MSFAELEAQIDSERIKAVMANKIAQGEVVSGKTPLGYSIENKKLVINDDAPIVIDIFN
                     YFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTNKKYIGELRNNKNYCPPIIDKKLFY
                     AVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVAGQTIKARYKKKDGT"
BASE COUNT          243 a           85 c          106 g          193 t
ORIGIN      
        1 gatggtttag aaatctacga cattatttaa acactcaaga aattttagaa aaacacaatg
       61 tttcttggaa tgctgtttct caacaatatt atgatacaac tacagcgtat ggcagaactt
      121 tcattgcaca agtaatgagt tttgctgaat tggaagccca aattgattca gaacgcatca
      181 aagctgttat ggcaaataaa attgcacaag gtgaagtagt aagcgggaaa actccgttag
      241 gctactctat agaaaataag aaattagtaa taaatgatga tgctcctatc gtgatagata
      301 tattcaacta ctttctttct agtggtagtt taagaaaaac tgtttattat ttaggctctc
      361 agtatggaat cgttagagat tatcaaagtg ttaaaaatat gttgacgaac aaaaaataca
      421 ttggtgaatt aagaaataat aaaaattact gtcctcctat tattgataaa aaactttttt
      481 atgctgttca aaaagcctta cctaaaaatt taaaaacaaa tgcaaaacgt gattacattt
      541 ttaaaggttt attaaaatgt tctgactgtc agggatcagt agctggtcaa actattaaag
      601 caagatataa gaaaaaagac ggtacag
//
LOCUS       DACEMO010000387          616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.429, whole genome shotgun sequence.
ACCESSION   DACEMO010000387 DACEMO010000000
VERSION     DACEMO010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.429"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..540)
                     /locus_tag="IW728_002834"
     CDS             complement(<1..540)
                     /locus_tag="IW728_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1026150.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEVRELPKSN"
BASE COUNT          178 a          100 c          100 g          238 t
ORIGIN      
        1 attagattta ggtagttcgc gaacttcttc tttgcataac aaagaaatat aatatttacc
       61 gctagaatga cgtgatattg tagcggattt gataatccct tttggttgtc tatgaagctt
      121 aattcttact aatgacttca atttaggaac tttgatgaat ttactatcaa tcaaagcaac
      181 tgtaccattt tgattattcg ttgtataact ctgaacagga tttttcttac ttttgaaacg
      241 tggaaatcca acggatttat cccgaaaaaa attcttgtat gctttatcta aattaagttg
      301 ggcattggct aaagcaaggc tatcaatttc tttcaaaaac ggaaattctt tcttgtattt
      361 agctggtgtc ggaaaagtca tttttttaga agaatcattt ttaacttctt catacgcctt
      421 ttttcggtca tcaagcatta gattgtagac cttacggaca caaccaaaag atttggcaaa
      481 gaagatttct tgttcctctg ttggatagat tctgaatttg tatgctttta gttgttccat
      541 aaagttcacc tactttctat ttatgattgt ttccttgatt ttggatatac ttcttaatca
      601 catcaattgg tgcgcc
//
LOCUS       DACEMO010000388          581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.435, whole genome shotgun sequence.
ACCESSION   DACEMO010000388 DACEMO010000000
VERSION     DACEMO010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.435"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..508
                     /locus_tag="IW728_002835"
     CDS             <1..508
                     /locus_tag="IW728_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAQ1026151.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          175 a          107 c          127 g          172 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aagatcgtca
      541 catccgagga ggctcattgt caaggacaat cggaatagca t
//
LOCUS       DACEMO010000389          555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.436, whole genome shotgun sequence.
ACCESSION   DACEMO010000389 DACEMO010000000
VERSION     DACEMO010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.436"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..424
                     /gene="tnpB"
                     /locus_tag="IW728_002836"
     CDS             <1..424
                     /gene="tnpB"
                     /locus_tag="IW728_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1026152.1"
                     /translation="KQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKG
                     MLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKS
                     LEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          200 a           91 c          109 g          155 t
ORIGIN      
        1 aaaacaaaaa cgcaaagtag ctagattgca tgaaaaagta atgaatcaac gtactgattt
       61 tctgaataag ttgagtacag aaatgatcaa aaatcacgat attatctgta ttgaagactt
      121 aaacacaaaa ggtatgttgc gaaatcataa attagctaaa agtatttctg atgtttcttg
      181 gtcaaagttt gtgactaaat tacaatacaa ggctgactgg tatggacgaa aaatcatcaa
      241 agtagataaa tggtttccgt ctagtcagat ttgttcagag tgcggacata aagatggcaa
      301 gaaatctctt gaaattcgag aatggacttg tcctgtttgt catactcatc atgatcgtga
      361 tatcaatgct agtatcaata ttctgaccga aggtctaaga ctacattcta tgggattggc
      421 ttagatccaa tcatcaagaa ccgtaggaac tacggggata gcttggtaaa taagagacac
      481 cgctgttagt aaagaaatac gctatcaagt atgctctatt cccaagaagc tcccacttca
      541 agcgttaaga accgt
//
LOCUS       DACEMO010000390          530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.445, whole genome shotgun sequence.
ACCESSION   DACEMO010000390 DACEMO010000000
VERSION     DACEMO010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.445"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>530
                     /locus_tag="IW728_002837"
     CDS             <1..>530
                     /locus_tag="IW728_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1026153.1"
                     /translation="KSYRSGELLKPDDKDGMGNTLYIGSLKSEVYFCLYEKDYEQYIK
                     LGIPLDQTETKNRFEIRLKNDRAYHAIQDLLKGRSIESTTFSIINRYLRFADKVEGKR
                     RTNWPLNEQWGRFIGRNQKEIQLTSEPKPYTIERTLNWLGRQVAPTWKMAKELDRLNQ
                     TTYIQDMVNNTRLSDR"
BASE COUNT          173 a           94 c          126 g          137 t
ORIGIN      
        1 aaaagctatc gttccgggga actgttgaaa cctgatgata aagatggtat gggcaatact
       61 ttgtatattg gcagtcttaa gagtgaggtc tatttctgtt tgtacgaaaa agactatgag
      121 caatatatca agttagggat tcctttagac cagaccgaaa cgaaaaatcg ttttgaaatc
      181 cgactgaaaa atgatcgagc ttatcatgca attcaagatt tgttaaaagg tcgcagtatt
      241 gaaagtacca cattttccat tattaaccgg tacttgcgat ttgcggataa ggtggaaggc
      301 aaaaggcgaa ctaactggcc cttgaatgaa caatgggggc gcttcattgg acggaatcag
      361 aaggaaattc aattaacttc tgaaccgaag ccttatacca ttgaacgaac gttgaactgg
      421 cttggacgac aagtcgcccc tacgtggaag atggccaagg agttggatcg gttgaatcag
      481 acaacgtaca ttcaggatat ggtaaataat actcgactat cagaccggca
//
LOCUS       DACEMO010000391          512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.449, whole genome shotgun sequence.
ACCESSION   DACEMO010000391 DACEMO010000000
VERSION     DACEMO010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.449"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            94..>512
                     /locus_tag="IW728_002838"
     CDS             94..>512
                     /locus_tag="IW728_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1026154.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLA"
BASE COUNT          175 a           84 c          107 g          146 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gc
//
LOCUS       DACEMO010000392          506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.denovo.450, whole genome shotgun sequence.
ACCESSION   DACEMO010000392 DACEMO010000000
VERSION     DACEMO010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.denovo.450"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..>506
                     /locus_tag="IW728_002839"
     CDS             4..>506
                     /locus_tag="IW728_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1026155.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLA"
BASE COUNT          170 a           94 c          109 g          133 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagc
//
LOCUS       DACEMO010000393        17297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.guided.039, whole genome shotgun sequence.
ACCESSION   DACEMO010000393 DACEMO010000000
VERSION     DACEMO010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.guided.039"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..291)
                     /locus_tag="IW728_002840"
     CDS             complement(<1..291)
                     /locus_tag="IW728_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1026156.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSS"
     gene            889..1752
                     /locus_tag="IW728_002841"
     CDS             889..1752
                     /locus_tag="IW728_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1026157.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            1852..2334
                     /locus_tag="IW728_002842"
     CDS             1852..2334
                     /locus_tag="IW728_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1026158.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2597..3064)
                     /locus_tag="IW728_002843"
     CDS             complement(2597..3064)
                     /locus_tag="IW728_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1026159.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            3187..4038
                     /locus_tag="IW728_002844"
     CDS             3187..4038
                     /locus_tag="IW728_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1026160.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(4289..6247)
                     /locus_tag="IW728_002845"
     CDS             complement(4289..6247)
                     /locus_tag="IW728_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1026161.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(6244..7116)
                     /locus_tag="IW728_002846"
     CDS             complement(6244..7116)
                     /locus_tag="IW728_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1026162.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(7458..8330)
                     /locus_tag="IW728_002847"
     CDS             complement(7458..8330)
                     /locus_tag="IW728_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1026163.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            8458..9027
                     /locus_tag="IW728_002848"
     CDS             8458..9027
                     /locus_tag="IW728_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1026164.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            9094..9831
                     /locus_tag="IW728_002849"
     CDS             9094..9831
                     /locus_tag="IW728_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1026165.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            10062..11330
                     /locus_tag="IW728_002850"
     CDS             10062..11330
                     /locus_tag="IW728_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1026166.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            11817..12281
                     /locus_tag="IW728_002851"
     CDS             11817..12281
                     /locus_tag="IW728_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1026167.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            12265..12894
                     /locus_tag="IW728_002852"
     CDS             12265..12894
                     /locus_tag="IW728_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1026168.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            13248..14726
                     /gene="msr"
                     /locus_tag="IW728_002853"
     CDS             13248..14726
                     /gene="msr"
                     /locus_tag="IW728_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1026169.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            14773..15219
                     /locus_tag="IW728_002854"
     CDS             14773..15219
                     /locus_tag="IW728_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1026170.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15418..15933)
                     /locus_tag="IW728_002855"
     CDS             complement(15418..15933)
                     /locus_tag="IW728_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1026171.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            16050..17251
                     /locus_tag="IW728_002856"
                     /pseudo
     CDS             16050..17251
                     /locus_tag="IW728_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         5467 a         3068 c         3572 g         5190 t
ORIGIN      
        1 ggacgagagt tcggaaagtg agtagttgga tttttttatt ggacgttctt cgtaagcgaa
       61 taagacagca gccatgtgtt tgcatttttt tccttctatg gcatatagac aatcacaagt
      121 catgttggtc ttttcatccc aagtgatcac taccttataa tcttccgtgc cagaaactgt
      181 tgcggacaag tacttgcttg tatagttcaa atgacggaca aaaccgtgca gatgatagct
      241 gtaaccgcgt tccaaaacgt tttcaggaaa atactcaagc caattattca tctagttctc
      301 ctttcatgaa acacttattt atatttagta taaagaaacg tttaagaaaa aagtagctga
      361 ttttacatat ttgataatca cttttgctcg aattgtcaaa aattcattct ccttttcttt
      421 tcttagagaa tgtgagaaaa agtaaaagaa tagcgtgttt taaagatgga actttttttg
      481 ttttttattt atctgacaaa aatgaagtga ttttgttaga aataaataat tgttccaatt
      541 gataaaagag gtatgatgta tcgcaaagaa aaatccaagc agcgttctga tcagcagttt
      601 gaacaacaat ttgctttgta cagtaggtag taagcggatt aactaaggga ataaaagata
      661 tttttaccgc tttgtttact tagaatctgt cttccccaaa atttattttt ctaaaggtaa
      721 aacatagata ggcagataat accaattatc cgttacgaaa cgctgcttga gaattttctt
      781 tagatccata caacattttc tacatctgac taaattggtt cctgattcat caagtaattg
      841 actttctttg ataatatgat aaaaaggcaa tcaaggagga agataaatat gcttaaagat
      901 ctgaaccaat tgatcgatta catcgaagaa catttgacag aaggactttc ctataaaata
      961 atagccgaaa atacaggaat ctctgaatat caattaaaac gcatctttac gtttattgca
     1021 gggatgtcgc taatggacta tatcaaaaac cgtagattgg ctttggctaa tcgcgatctt
     1081 ctagatggat caagagtgac agaaacggcg tttagatatg gatatgaaac agtcgaaggt
     1141 ttttcaagag cttttcgtga ttggtcagga tatctgcctt cagaaatcag tagatacggc
     1201 atacaaaaat cttttccacg cttgacattt tttattgatg tcagaggagg aaaatcgatg
     1261 gaatttcgta tcgaaaagaa agaacagttt tatgtggcag gtgtggcgaa aaaagttcct
     1321 gtcaaatttg aaggagtcaa tcaagaaata caaaagttag cgcagacgat cacggagact
     1381 caaagggaag agatgcataa gttaggagat ttatatccaa atcaagtaat caacgtttct
     1441 tatggatttg atggagaccg cttggaagaa aagggcagtt tgatgcacat gattggcttt
     1501 gctacgacaa aagaaaatcc gtacgctgat ttagaaatgc ttgctataga agaacacact
     1561 tgggccattt ttcctaataa agggccattt ccccaaactt tacaagatac ttgggggaaa
     1621 atctatgcag aatggcttcc ttcttcagat tatgggttag tcgaagctcc cgagatctct
     1681 tttacaaaat ggggagagga tttcagtaat gtttacagtg agatttggat tgcagtgaaa
     1741 gcaaaaaaat agttaaaaaa taaaaatatc cctagaaaaa agagcgtaat cgtgtcattt
     1801 ccctttaatt ttatgtaaga tgaacttaat ataatagaaa gggtgggaga aatggcaaag
     1861 tataggatcg aagaaaaacc agattttacg gtgacgggta ttggtgtgga attgaagagt
     1921 agcaatacgg attttgtcgg tttggctgca gagaaacaag catttgagca aaaagttatc
     1981 ggtaatggaa caatagatgc attgaaacaa gctgctgaaa atcattattt ctttacagtc
     2041 aacgaaccct ataacgggaa aatgatgtac tatgtcggtg ttcaaacgag tcaggatgtt
     2101 ccaaacacta cgcgaatcat cccattccca aaaggaacgt atcttgttgt aagcgcttta
     2161 gcagaatcgc ctgaagaatt aagaaatgat ttgacgaata cggcctttgg gcatgtttta
     2221 agagaagtag aagattacgc ctatgttggg ggtccaaatg ctgtagtcga aatgggagtt
     2281 accaacggga aatatatggg agaaatctgg ataccggtag tcaataaacc atgataatat
     2341 agaggtcgtg aaagtagcgc tttgacctga gcattagaga ggaaaaatgg gaaatagctt
     2401 tccatatttt gcatttttca gaactaatgc cgagggtcag ctacttgaac accgtttatc
     2461 aagacgtcat gacttcagcg ctttgacctg agcattagag aggaaaaagg ggcatgacaa
     2521 ggcttttgtc ccccggagaa taaaagaatt tatgtagacg attccgatca gaaaaatgca
     2581 ggcacagtag agagaattat aaaaatctct ctacgaggcc tgtgttttct ggtctttttt
     2641 ctttttccaa aacggctatt ttatgtatca gactatcaag tgtgatctta gccatttctt
     2701 cttctgcctt tttctgcagc cgagcgtagc tttcttctaa agcttcttgg atattcgcac
     2761 caacgacgca atttggattt gtttttggat cgacttggat aagctgtgta ttaccttcga
     2821 tgctgcgata gacatctagt aaagaaatct gatctggact tttggcaagg gaaggctgcg
     2881 gttttccggt ttttgtaaag atcaaaccgg attttttgag ctgactcatg attttacgta
     2941 catttgctgc atttgtttcg atactactgg caatcatctc actagaaagt gatcgggtat
     3001 cttgaaacat ttcaatatag gcaagtacat ggattgcatc actaaattgc agagaatact
     3061 tcatcagttg tcatcctttc attttttttg cttgacttat cttaccacac tctttatgat
     3121 aaaggtgtaa tttataaatg tacagctgta aatcttaaca aataaaaaga atagggagtg
     3181 gcaaatatgc attatttagt aacaggagca actggaggtt ttggtggtta tgcgctggaa
     3241 ttcttaaaaa agatggttcc acaaaatgaa atctatgtac tagtacgtag tgaagaaaaa
     3301 ggagaagcac tgaaagaagc tggatttaat atacgtattg gtgattatga tgatttagac
     3361 tcaatgaaag aagcgttgaa aggaatagat cgattgcttt ttgtctctgg cgttccagga
     3421 aatcgtcaag ccgaacataa gaatgtagtg aaggcagcac aagcagctgg ggtatcttat
     3481 atcgcttata caagttttgc agatgccgat cactcgatca gtgtattagc gccagatcac
     3541 caattcactg aaaaaatgat taaggattca ggtatttccc atacattttt acggaataat
     3601 tggtatgtag agaatgaatt acctatcatt gggcaggctt taaaaactgg tcaattcgtt
     3661 tatgctgcag gaaatggaaa aacaggttgg gcattgaaac gtgaatatgc agaagctgca
     3721 gcaaaagtac tagtagaaag tgactctcca gaaatcttag aattatccgg caatctaact
     3781 gactatgaag aattagccaa ggcattagag cgtgcgacag gtaaagagtt agaaattatt
     3841 gaagcaagtg atgcagcgtt tgtcgaaaat ctaaaagaag ccggattccc tcaagaagca
     3901 gctgacatgt ttttagcgat ccaacatgat attaaaaagg atcagttagc tatttcctct
     3961 gatgatttag aaaaagtatt gggaaaacca ttaacttctc ttgaagcagc attaaaagaa
     4021 atattggatc aggaataatg tataaagagg ttgtgaaaaa gccgattagt tttgagtatg
     4081 aagaaaaatt ttgtgaaaaa gctgttctgc atcaagtaat aagaagagtc aaataaaaaa
     4141 tagctcttca tatgtttcgt ttagctggac ttattaccgc ggagttggca ggagaacgcc
     4201 gtttttattc ggaaaaagga gctgagacaa gatttttgtc tcagctcctg tttgtttatc
     4261 gataaaaaag tctggggaag acgatggctc aggctttgtt ggaaacagag ccgattttat
     4321 catttgcttt tacatcacgt gcgggttcga tttctaagct ttggactttg tcgccatttg
     4381 tgaatacaac gaccatatcc gtgtttttgc ctgcagattg aatagcggca agatcaacta
     4441 aagcaatcaa ttgacctctt gcgacttttt gaccttcttc tacatataaa gtgaatggtt
     4501 cgccttttaa gtcaacagtg ttgatgccca tatgaagcaa gacttcgata ccagcatctg
     4561 tttggattcc gacagcatgt ttcgttggga aaatgtttgt gatcgtacca gcgattggag
     4621 agaagatttc tccattttct ggtaatacag catagccgtc acccatcatt ttttccgcga
     4681 atacatcatc tgaaacttca gtgattggaa tcagtttccc atttgcgata gcgtaaatat
     4741 cttgttcgat cgtttcaccg gcaaatgttt ctgttttaac cacggtgttt gtttgtgcag
     4801 gtcgtgctgc agcaccacca tgtagttttg ccatctcatc tgcaacgaat tgtacttctg
     4861 tacctacgat gacttgcgca ttttttccgt cgatgatctt cataccaggt acgccagtgg
     4921 ctttgatttt attttgatcg atcaggcctg tatctttgac ttgcaaacga aggcgtgtag
     4981 tacagttatc gatcgaagtt acattgtcgg ctccgcctaa tgcagcgtag attttgcttg
     5041 cttgtgccgc atatttattg tctgaattag tcgttgcagt atcgtcatct gaaagatctg
     5101 cttcctcgcg gccaggagtc atcagatgga attttttgat agcaaagtta aagccaaagt
     5161 agtaaatcac tgctgttact aatccttgga cgatcaacat caatggctga ttcgcaatcg
     5221 gatttttcaa agataggaag taatcgacaa atccggcact aaaggcaaat cctgctgtcc
     5281 aatggaagaa tgcactcact gctaaagaaa tcccagtgaa aattgcatgc aagacatata
     5341 atggccaagc aacgaacata aatgaaaatt caagtggttc tgtcacacca gtaaagaatg
     5401 aagcaaaagc tgctgctagc atcaaggaag ctgtttttgc tttgttttct ggacgtgcat
     5461 ttctgtaaat agcatatgct cctgctggaa gcccgaacat catgattggg aaaaatccag
     5521 cttgatacat tcctgttacg ccttttgttc cttcagaagc caaaaattta ccaatatcat
     5581 tgattcctgc tacgtcaaac cagaacacgg agttcattgc ttgatgcaat cctgtaggga
     5641 tcagtaaacg gttgaagaag ccataaagac ctgcaccacc ccagcctaaa ctcaaaatga
     5701 atttagcaaa agaaactaaa gcagagtaaa ccggaggcca tacgagcagt agcactgcac
     5761 tgatcagcaa catgacaaag gcagccaaga ttggcactag acgcttgccg ctaaagaaag
     5821 ataaagccat cggtaatttt gcttcgtgga aacgattata aagagctgcc gcaatcaatc
     5881 cggcaataat accaataaat acgttactga tctgagcaaa agatggatca actgattctg
     5941 gtttgatatt caataaagta gctactgaat cagtttttaa aacatttact ggaacttcaa
     6001 atgcgaccaa acctgccaag gcagcagcac ctgatttgtc cactgacatc cctagtgcta
     6061 acccaacggc aaacagcaaa ggtaattgac ctaaaactgc gttaccgcca gcttgtaaaa
     6121 aatgagcgac aatgtcgtta ctataagatg cccaccagtt tccgatccct actagtaaag
     6181 cagcagcagg taagaccgca actggaagtt gaagggaacg tcccattcgt tgtagatatg
     6241 ctttcaattt ttttcctcct catgattgtt aacatttgtt tgtacgattg cgcttcttaa
     6301 acgctcgata tggatggcca agtaacccag ttcttctttc gtgatcgcga gctggtattc
     6361 tttggtcagc aacacacgga tttttttaga aatcttatag ctttctgggt attttcgttt
     6421 gaccatttct acgatttccg tgtctaaaac agcatattgt tgcgcatggg ttcgttgtag
     6481 aagcagtcgt aaatgattca ccagtcttga ataattcagt gaaaatggtt ctgaatggat
     6541 atctgtttcc aattctgatt ccactaaatg gatgatgtca gatacaatcg tcacttcacg
     6601 gatactgcga tggttattgt tccgaccgct tcgtccgctg tgaatatgga ttgcgatata
     6661 tcctgcttcg tcataagtat atggaatccc aagctctttt gtcagatact ctactgccca
     6721 ttgagcaata ctgaattcat cactgtaaag cacttcgatt tctcttaaaa gtttgttgcg
     6781 gatgacgata ccgttattaa tgttctccgc agaaaaagca atgtggtcgg tcaaagcgat
     6841 caatacatgt tcattcaact tttccatcaa aacagtttcc gcatgatcaa tgatcgtttc
     6901 tgctgcaaat aaaaagcgtt catcaatctg attaagtaaa ttttgcaact tgatttgacc
     6961 ttctggttcc atgacaaata tccgttcgac atcacgatca aaaatcaaat catttctttt
     7021 tctgtcaaat ccaatccctt ttccaatagc tactttttcc tgtccaccgt catctaccaa
     7081 tactgcattt tgattaaaga tctttttgat tttcatgcgc gcaccctttt tgaagatttg
     7141 gttttcacgt ttgctagacc agttgattgc ggccaaacaa aaatagacac gaaaaacctc
     7201 ctagggaaac catgttcctc agagattttt catgcctagt atgatctagt cacatgaaac
     7261 gaattattgt taacgtaatc aattttatat agacagttat gttttgtcaa ttcttttttt
     7321 ataattatag taatttactg gaattatcag aaataaaagg aagaaataaa ttaagaaact
     7381 tatgttattt aatcgttgga tcttttgctt atcttattcg ttgttaaaaa gctgaaagcg
     7441 aaggaaaaaa actctactta actgtatttc tccgttagga catctaaaaa tttacgagca
     7501 gcaggagaaa gaggctggtc ttttttccaa atgagattca gtgaagcttg taatggcgga
     7561 tcgagtggga tgaatatttt ttccgtccca taagtattga tgatcccatc tagagctaat
     7621 gcaccaaaag tcccgttatc gaccatcaaa gaagcattgt acaataagtt atatgttccg
     7681 acgatcgata actgatctat gctgaaacct agccattcag ctagttgact gtccactgac
     7741 gtttgccggg aaatgatcaa aggaacagtt ttcagttcct gcggatggat gacggtttgc
     7801 tttgctaaag gatgatttcg atgtatcaaa agtccccaac gatcttgggc tggcagtcga
     7861 aatgactggt atttttgttt gtttgtcggt tcgattacga gtccaaaatc aagtaatccg
     7921 ctatccagtt tgtctatgat atcatctgca ttcccgctat ataaatgaaa ctggatatca
     7981 ggatgcgcct tgatcaacga atgaaggcta tcagaaatga aagagattgc tttagtttcg
     8041 cctccgccaa tgtagatttc tccagagatg attgcgtcat ttgttaaatt gttcgccgtt
     8101 ttttctacca acgacaagat ttcttttcct tttgaataaa gatatcgacc agaatccgtc
     8161 aactgtatct ctcgggagcc ccgagtgaaa agtgtcacac caagttcttc ttctaaatct
     8221 ttcagttgtt ttgagagagt aggttgcgtg atatgcagag attttgctgc tcggctgata
     8281 tttttttctc tagctactgc taaaaaatac gtcagtatac gaatatccat tttttccctc
     8341 ctgtttttaa aaaagcatac cactaagtat agttaaaagg aatagttaag tatgttttat
     8401 aagtattaga catgataagc cgtccgttat aaagtaagga taaaaggagg gagcaaaatg
     8461 acaagcctca tcgaaaaaca aatcgtcaat aaagagattc gagtaacgga tccgctcttt
     8521 gaagtgatcc atcagatcca agctgaaaac gaagagcctt tagcagcgct gaatactggg
     8581 taccatgaac cagcagatat ccgcaaacgg ctagaaaata tcattagtga caaagtagat
     8641 gatacggtga ctgttctgct gcctttttat acagatttcg gcaaacatat tagtataggg
     8701 aaaaatgtct ttatcaatcg tcaagtgatg ttcgttgact taggtggcat ctgtttagaa
     8761 gattcagtat tgattggtcc tcgtgtcaac ctcatcacgg tgaaccatct aaccgatcca
     8821 aaagaacgaa gaggcttgtc agtaaagcca atccacatca agaaaaatgc ttggatcgga
     8881 gcaggagcaa ctattctgcc aggcgtaacg attggcgaga atgccattgt tgcagcgaat
     8941 gcaactgtta cgaaagatgt cccagacaat accatcgttg caggaatccc tgccaaaatc
     9001 gtcaaacctg tcgaacggat tatttgaaca gtaagatgaa ataagagata gtgagtataa
     9061 gcaagagatt tttcaaaaca ggaggagaat agaatgaatg atttatcaga aaaagtgatt
     9121 gtcatcatgg gcgcttcaag cggaatcggt gaagcaacaa cgaagctatt agcagaaaaa
     9181 ggggctaaac tagttatagc tgcacgccga gaagatcgcc taaaagcaat aaaagaatct
     9241 ttaccagaag ctgagctata tattcaaaca gcagatgtaa gagattttgc acaagtccaa
     9301 gcagttatcg atttagcgat ggaaaagttc ggtcgaatcg atgtattgta caataatgca
     9361 ggaatcatgc caaccgctcc attagttgaa ggtcaccgtg atgaatggca aaatatgctt
     9421 gatatcaaca tcatgggtgt gttaaatgga atttccgccg tacttccaat catggaaaaa
     9481 caaaaatctg ggcatatcat ttcaactgat tctgtggcgg gacatgttgt ttatcctgat
     9541 tccgccgttt attgcggaac aaaatttgct gtccgtgcaa tcatggaagg attacgtcaa
     9601 gagcaacggc aaaataacat caaatctacg atcatctctc caggagctgt tcaaactgaa
     9661 ttatatcaaa ccatcagcga caaaaaggct gctcttgaac ttcatgaagc gcaaaaagaa
     9721 tggggattga cctccgaaga tatagcttct gctgtagcat ttgcgataga aacgccagac
     9781 cggatgtctg tcagtaacat gattatccgt ccgacgacac aggaagtata atccataacg
     9841 gataatatcc aaagagaatt acttgttaaa tagttattca aacgaccatc aatttaaggc
     9901 gtgaatggag aagaatattc ttcatttacg tctttttatg tagaaaaagc aaatttatca
     9961 aaaagaaaca aatgttattt aaatatggac attgacttgc ttttactttt ttagttacac
    10021 tgttatttag gaaaagtttc ataattcaaa ggagaaaaaa gatgaaagca aagaatttat
    10081 tatttagcgt tattctttta ggaaatctag cagttgccaa cacaacagtg ctagctgaag
    10141 aatcgacaga aactgtcgca actcagacat cgagttcttc agaaacagga attgaaaacg
    10201 atacagagag tactttgcag tcagctactg tctcagaaaa tacaatttca accgaagaac
    10261 caacaactga aacaacagaa caaacacaaa ccgaagacag tacatcagca gaagctcaga
    10321 acaatccaga gaatgcagaa gaaatcacga accaattgac agatgggcaa acattgacac
    10381 atacccatca agcagatttg tctgaattcg aaaactatac tggctatgct ttttatgctc
    10441 gctcatctgc taattctcaa caagcgttca ttgatagtat tgcctcaact gcacaaagtt
    10501 tggcaagtgc caatgactta tatgcatcag tcatgattgc tcaagccatc gttgaaagtg
    10561 gctgggggaa cagtactttg gcttctgccc ctaattacaa tctatttggg attaaaggaa
    10621 gctataatgg ccaatctgtt acgatgccta cttcggaata tgtgaatggg caatggatta
    10681 cggtgaatgc cgcttttaga aaatatcctt cgtacaaaga atcattgcaa gataacgtaa
    10741 cggtattgaa gacgacttct tttcagccag gcgtttacta ctattccggt gcatggaaaa
    10801 gcaatacgaa ctcttacaaa gatgctacgg cttggctaac aggtcgttat gccactgcgc
    10861 ctaattatgg ttctacattg aacaatgtaa tcgaaaccta taatctgaca cagtatgata
    10921 cagcaccaac ttcaacttct ccgatgtatc gtctgtataa ccgtcataca ggagaacatt
    10981 tatatacatt gaatgccgga gaaaaaaatt atctgccaac tgtaggctgg gaatatgaag
    11041 ggattgcttg gcaagcacca aacagcggac aacctgtcta ccgtttatac aatccttaca
    11101 gtggagacca tcattacaca atggcacaat ctgagatcaa ctctttaaca aaaatcggtt
    11161 ggcgttatga aggtctaagt ttttattctg gtggttccaa gccaatttat cgactattta
    11221 atccaaatga aaaaactggt acccatcact ataccttgag tgctaaggaa agagattggc
    11281 taacaccgat gggctggaga tatgaaggaa taggatttta tggttattaa aaagaggtag
    11341 tgaaaaagct gccctgcatc aagtagtaag aacagccaaa caaaaacaga ttttcatgtt
    11401 ttttgtttgg ttgaacttat taccgcagat gcaagttttt gaacaccgtt tactcaaaaa
    11461 aggttgtgac aacatcttgt cataactttt ttattactgt ttaagcatac tgatccaatt
    11521 tctaatattc gacgggattc ttgcaaaagg cgatttcgga aatttttggc ggtgtaaaat
    11581 tttctactgc agaagagcta tcttctatag tttgataaga atcaataatc ttggttttag
    11641 cgttgtgctt tctttatcat tttttccgtt cattctacaa aaaatttctt ttctctatac
    11701 ttttctattg agagcgtttt ttttattaca cagctgattc tttgactttt ttcttttttt
    11761 attctattat tgacaagtca atcgttgacg agtcaattat tagaaaggag gaaattatgt
    11821 ctttagaaga gattagtcac cttttatatc aaatcaaatt agccgatcaa aaaatcgtcc
    11881 agatctttga agaaaatatc ggtataagtc ttactagata cgagatcatg atgagactga
    11941 aagaagaaaa tccattgcta caaagcagtt tgcaagaaaa gttgcagatt gatcaaggag
    12001 cagttactcg tcatctgaag gtattggaag caaatggcta tgtttcgcgt cagagaaatc
    12061 ctcagaacaa ccgggaagtg tatgtagcgc taacagaaaa agcccagaag aaaatcgagc
    12121 attgtgaaat ggaccataaa gatacaggtt ccgtgctatc cccggctttc agcgatgtag
    12181 aaatgaaaca atttctaaca cttttagata aattgaatac ccaactttgc aatataacaa
    12241 cagaaacgaa agaggttaat aataatggaa caaaaaaatg atttttcaac aattatgact
    12301 ggtagaagat ccgttcgcgt atatgatgaa acatacaaaa tcccacacga agaaatgctg
    12361 gaaatgatca aagaagcgac gactgcacct tcttctgtca atatgcaacc atggcgcttt
    12421 gtggtcgctg aaagcgagga agcaaaagaa atcttgcgtc ctttgatccg tttcaatacg
    12481 agacaaaatg atacttcttc tgcaatggtc atgatttttg gcgatatgca atgttacgaa
    12541 tatggcgaag aaatctataa ccaagcttac gaatcaggga agatgccaaa agaagtcaga
    12601 gatcaacaat tagctgcgat cattccttat tacaagagtt tttcaagaga agaaatgaac
    12661 gatgtagtca aagtcgattc tagcttagcg gctatgcaat tcatgttagt tgcgcgtgat
    12721 catggctatg aaacaaatcc aattggtgga ttcgaagcag atcagttggc agaggcattt
    12781 ggtttggata aagaccgtta tgttccagtt attattttat caataggaaa agcggtagaa
    12841 gaaggttacg agtctgttcg tttagcacca gaaaagatca ctacgttcga ataaaaggtt
    12901 ttcagttact attcagtagc tattgctttt tttgaatcgt gtgtggtaac ctaataaaaa
    12961 aggaggtcgg aaaaatgact gcatcgatga aattacgttt cgaacttttg aataacaact
    13021 aaataaattc aaaagacatg tataaacaac aatttcatgg atggagtatg tccatttttt
    13081 cgaacgacag tagaataggt gaaagctatg acagatttgg tgatttagtc atagttattt
    13141 cgttcagagt aggaaaagat gtgatgcagt ttttttgcaa acagattttt ctgctctttt
    13201 ttagtatcga taattgttgt tttacatgaa ataggagtgt aaaacatatg gaaaatttag
    13261 cagtaaatat aacaaatcta caagtcagct ttggcaacca gctagaacta tctattgatt
    13321 ctcttcgtgt ctatcagcaa gatcgtatag ggatcatagg agaaaatggt gtcggtaaat
    13381 cgactttgct caaactaata gccggtgaac tttttcccga tcatgggaaa atccaaacag
    13441 agatcacctt caactacctg cctcaattaa cctatctcgc tgaagcaaag gacctaaatt
    13501 tggaattagc cagtcatttc cagttaagac tggaagaaac ttcagagcgg aaatggagcg
    13561 gaggggaaga acgaaagatc gagttgatac gtcttctttc ttcttatgaa caagggatgc
    13621 ttctagacga gccaacaacc catctagata gaaaaagtat tgatcgactg attgaagagc
    13681 ttcgttatta ttatggtacg ctggtttttg ttagtcatga tcgctatttt ctagatgagc
    13741 tagcatcgaa aatctgggaa gtaaaagacg gagaaatccg agagttttcg gggaattata
    13801 gtgcctatct cactcaaaag gaattggaga aaaagactca gctacgagaa gcagagtcga
    13861 tcatgaaaga gaaaaaacga ttggaaaaat cgatccaaga aaagaaaaaa caagcggaaa
    13921 agttggaaaa agtgtccagt aaaaagaaaa agcaacaaat cagaccggat cggttgtctt
    13981 cctctaaaca aaaagacagt gtacaaaaag ccatccaaaa gaatgcgaaa acattagaga
    14041 gaagactcca aaaaatagga gaaacaacca agccgcagca gatgaaacaa atccgttttc
    14101 cagtaccaaa atctcttgaa ctccacagcc gctatcccat catgggacaa aatgtccaat
    14161 tggaaagaag tgggagaaca ttactggtaa atggtgattt tcagttttct ttaggtaaaa
    14221 aaatcgcgat tgtcggtgaa aatggttcag gtaagacaac cttattagaa catatccgca
    14281 aacaaggaga aggaatcctt ctctctccga aagtaagctt tcaagtatat cagcaaaagg
    14341 gttatcaaat gacatctgaa gaatccatca ttcgttttgt catgagacaa acagagtttt
    14401 cagaatcgct tgtccgtagt ttgctgaatc acttagggtt tgctcaggaa actttgacga
    14461 aaccgttatg tacattaagt gggggagaag cgacccgtct gacgattgct ttgcttttta
    14521 ctaagccaag taatgtgttg ctgttagatg aaccgactaa ttttattgat atggcaacga
    14581 tcgaagcttt agagaagcta atgcaaatat atccgggaac gattttgttt acttctcatg
    14641 attcttactt tgtcgagcgt acggctgatg aagtttatga aataaaaggg cagaaaataa
    14701 aaaaagtact tacgagaaat ttttaaatgt gctacgatga ttttcgtgac aaatttaaag
    14761 gagtggtaca gattgcctac aaataaaaaa gaaggaataa tttttacaac aatgatgtgt
    14821 tttttgatgg tcttggggat gagcgcgtat aatttatggc tgcatcaaga tcttcattta
    14881 tcaagtttgt tgattggtct agtgcctgga ttcgtggtgg ctttcatttt ggatgtcttc
    14941 atcatcggtg tgctggcgaa aaaaattgcc tttaaactac cagtaaatcc agaaagcaag
    15001 gtccaaatga tcttaactat ttcatgcttg atggttttag gaatggtgac atgtatgtct
    15061 ttattcggtg tgatcatgga acaaggaata cctgatcaat tattctctgc ttatatgaat
    15121 gcttggaaaa tgaatgtgat tgccgcttta ccgttgcagt tgatcatcgt tggcccgctt
    15181 tcaagaagtg tgctaaaagc aattcaaagt aatgaatgat ggaacactgt tatttgaaaa
    15241 aaggccttgg catgcttttt gtcatagtct cttgaatgca gttctagaaa aatagtgatc
    15301 cgaacgatag gttttggcct gtcgttcgga tcgctttttc ttttccaact gtattgctct
    15361 tttattgaag ataatttgct cttcaataga tatttctaga aaggttctgt ttatccctta
    15421 ctcaaaaaac tgctgcattt gttctcttac ttgctgacac cattggagtt cctgctgaca
    15481 aagcataatt ctgcgctgga tcacaacata acgtccaaat tcttgtttct tgtctagcga
    15541 gaggtcattc aatttttttt gatttttttg catgattttg ttcagctgtt gttctctagt
    15601 gaatagcagt ccagtagctg ttgttttatc gagcgtcgaa aaagcataga tttttgctag
    15661 aaattcatct ttttgcgtag gagcgggagt ttcctctttg atccattcct ctacaagagc
    15721 acgtccttca tcagtcaagt ggtagatctt tttttgagag tgctcgtctt tgtccaaaac
    15781 ggtcacataa ccctgctggt ctaacttggc aagcgttgta tagatctgac tgtgatgggc
    15841 ttcccaaaaa attgtgatca gctgcttcaa ttcataccca gaacgtggtt tggataaaag
    15901 cacgcataat aatatatagg ctaaagtatt cataaaaatc ctacctctcg tcttattttc
    15961 tttattatat cacagtaaag tttccacgtt ttacatgtgc atatttacat gtaaaataaa
    16021 tcatgtatga tgctaataga gagaaaggga tggaaatatt ggagaatcaa atagatatac
    16081 aagaacaaac aaatgaagaa acaaagggga aaagttcagc gctagttgta gcagctacct
    16141 gtatgatcgc ttttatggga gtgggtctag tagacccaat cttaaaaacg attgctgcac
    16201 agttaaacgc tacgccagct gaaacgaccc tgttatttac tagttacatg ttagtcactg
    16261 gtgtggtgat gctatttaca ggtttcttat ctagtcggat cggtttgaaa aaacattgat
    16321 gacaggattg tttatcatcg ttgtattcgc tggtcttggc ggattatctg ggaatatcgg
    16381 aatgctgatt gggttacgtg ccggctgggg tgtcgggaat gctttatttg tctctactgc
    16441 tttagcaacg atcgtcagta ttttagttgg taacacagaa aaagcaatca tgatgtatga
    16501 agcagcactt ggctgcggaa tggctgttgg ccctctagtc ggtggggtct tgggcagtgg
    16561 ttcatggcgt tatccatttt ttggggtagc atttttgatg ttttgcggtt ttatagccct
    16621 agcggtactt ttacctgaaa ctgagaaacc aaagagaaaa gtaggccttt gggaaggtgt
    16681 caaagcgtta agcaatcata aacttcgttc agttggacta atcgcattgt tatataattt
    16741 cggattcttt actttacttg cgtatgcgcc atttttattg attggctatt ctgaattaga
    16801 agtcggtttt gtattttttg gctggggtgt gcttctggct attgcttcga ttttcgttgc
    16861 accggctctt gagcggaagt ttacgactta tcggacaatt ttagcagcat ttgtcttatt
    16921 cattatttgt ttgctgttat taggagctgg aacttctatg ccagtccttg taccgatcag
    16981 cattgtgtta gcaggatttt tccaaggaat tatcaataca ctgttgacga cgattgctat
    17041 ggaaatacca ggtctgcaac gaaacgttgc ttcgtccagc tatagttttg tccgcttttt
    17101 cggtggagct ttagcaccat ttattgcagg tgtgattggt gaacatattg gtgaaagcta
    17161 tgcgttttat tttgcaggtg ccattgttct tatcagtgta tgttgtatca tttatcaccg
    17221 taattatttt gctacaaatg aagaagaata agaaatcaga ggttgtgaaa aagctgtcct
    17281 gcatcaagtc ataagaa
//
LOCUS       DACEMO010000394        12271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.guided.074, whole genome shotgun sequence.
ACCESSION   DACEMO010000394 DACEMO010000000
VERSION     DACEMO010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.guided.074"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(44..1384)
                     /locus_tag="IW728_002857"
     CDS             complement(44..1384)
                     /locus_tag="IW728_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1026172.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1653..2549
                     /locus_tag="IW728_002858"
     CDS             1653..2549
                     /locus_tag="IW728_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1026173.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(2699..3640)
                     /locus_tag="IW728_002859"
     CDS             complement(2699..3640)
                     /locus_tag="IW728_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1026174.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            3766..4887
                     /locus_tag="IW728_002860"
     CDS             3766..4887
                     /locus_tag="IW728_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1026175.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5145..6647
                     /locus_tag="IW728_002861"
     CDS             5145..6647
                     /locus_tag="IW728_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1026176.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            6714..7241
                     /locus_tag="IW728_002862"
     CDS             6714..7241
                     /locus_tag="IW728_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1026177.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            7452..8513
                     /locus_tag="IW728_002863"
     CDS             7452..8513
                     /locus_tag="IW728_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1026178.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            8516..9412
                     /gene="murQ"
                     /locus_tag="IW728_002864"
     CDS             8516..9412
                     /gene="murQ"
                     /locus_tag="IW728_002864"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1026179.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            9426..11408
                     /locus_tag="IW728_002865"
     CDS             9426..11408
                     /locus_tag="IW728_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1026180.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11473..11919)
                     /gene="nrdI"
                     /locus_tag="IW728_002866"
     CDS             complement(11473..11919)
                     /gene="nrdI"
                     /locus_tag="IW728_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1026181.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12130..>12271
                     /locus_tag="IW728_002867"
     CDS             12130..>12271
                     /locus_tag="IW728_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine:ornithine antiporter"
                     /protein_id="HAQ1026182.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSW"
BASE COUNT         4064 a         2202 c         2588 g         3417 t
ORIGIN      
        1 ctccatctat tccacataaa taaaataaaa ttttctatcg atttcattca ttcgtcaata
       61 cgaacgcatc attctttttt tgataactga aataaatgaa caggtaaagc aggccagtca
      121 atacagttac cccaatggag ttcgtgaaca ggataccaag gaaaatcaat cctaagacag
      181 ctaatgcaaa aaagctgatt cctttatccc ataatgttcc ttttcttagt gccagtacaa
      241 aggatgccgc actgataatg atccagatca ataatactgt atacgaaagg gaacctgcca
      301 gatagccaaa taatttgtca ccgacaaaat aagaaagagc tacaccaaga taaagaacac
      361 tggtacaaaa taaaacagca cgttgcggta cttgatgctt gtttaaaaca gctagttttc
      421 tagaggaacc ttttcgatcc ttcaaacgga agaacaaaat tcttgaagat gcatacacac
      481 ccgaattgat agatgagaac aaagcaagga caatcacaaa attgacgata tccgctgcaa
      541 atgggatatt cattttttca aataccatca caaaaggact gacattcgtg cctgctaaag
      601 tattccaagg aaaaagaatc aaaagcaaaa acaaaggaat gatgtaaaag gaaatgattc
      661 ttcccatgac acctttgatt gctttaggaa tcgcacgttt aggatcatct gcttcgctta
      721 ccgtgatagc aatcagttca gaaccgccgt aagaatagat aactacaagc aaagaactga
      781 tgattccctt cataccatga ggagtaaacc caccatggtc agtcaattgc tgaagggtag
      841 ggaaaacgcc tgtgcccaac atttgctgac cgacaagata agtagcaaaa atgatcagta
      901 agataatgac actgatttta gcaaaagcca gccaatattc tgtttctgcg aataaccgta
      961 ccgaataaag attgatgatc gtagttaaaa cagccaacat aaaaacaaat acccaggctg
     1021 gaatgtgcgg gaaccacaac tgcaaaaagg aagcagctgc aacagcttca gcgatgatat
     1081 tgataatcca catagaccag tagacccaat cagtaaaatc agcagaatga gcacctagat
     1141 aagggcgtat caaaccagat aatccgtgat gcgtgtcgcc accgcttaaa acgatttttc
     1201 ccagaccttt catcacgata tataaaatca gcccaccaat caaaaaagca atcaatacag
     1261 aagggccggc gatcccaatc gcttcgccac ttcctttaaa tagacctgca ccgattgctc
     1321 cacctaaagc taacatggtg atatgtctgg agctaagttg ttttttcaat tttttatctt
     1381 ccataaatga cctctttctt ttaaattcac aatcaaaatt tatattaaga ataaatagca
     1441 atatttaaca atgactaatg ataacaaatt ttcagacaat tacaactatt cagaaaactt
     1501 ttaggcgaat tttaatagaa gaatgattta tttcatcata ttttcataca aatgttaaat
     1561 tttcatacaa atataggaaa cgttttactg gctttttact atttaaacag gctatcatag
     1621 gaaaaagtga cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg
     1681 caaaaaataa atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa
     1741 aagatttact acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc
     1801 tgatcgtttt tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta
     1861 ccaatgcaaa aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc
     1921 tgaagcaggc acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag
     1981 caaaagaact tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt
     2041 ctagcgaacc aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag
     2101 cagaaagcta taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc
     2161 cacatcgagg gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag
     2221 gggaatcgcg cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga
     2281 catttgaatc catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt
     2341 atcattttta tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac
     2401 cggaatttct gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg
     2461 cagaaaattg ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat
     2521 atgacctctc ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg
     2581 aaacaaacaa gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta
     2641 ttgggcttaa aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt
     2701 attctgcgcc aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg
     2761 gtgtagcaat atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt
     2821 cagtcaaacg atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt
     2881 tgtcaggatc ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc
     2941 cttcttttgc tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg
     3001 ctttttgcgc gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg
     3061 ttccatttac acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat
     3121 ttaaatttgc ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt
     3181 ccaagttctc tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta
     3241 gaccagctgt ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca
     3301 gtacatcttc atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa
     3361 tggattggat cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca
     3421 agtccacgtg ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga
     3481 ccacttcttt accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag
     3541 caggccaacg atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc
     3601 ggctgcccat tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc
     3661 aatgagttcc cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa
     3721 aaatgaaaaa aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg
     3781 gcaattgatt catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat
     3841 ggctgggaag atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat
     3901 actgcagaag caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat
     3961 ctcttattcc gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc
     4021 gcagtcatcg aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa
     4081 actatccgac ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa
     4141 gaagtagaac aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga
     4201 ttactccaat ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct
     4261 actaaaaaac ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc
     4321 gctgcagatg ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga
     4381 caaaaaggag gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct
     4441 cagcaaataa aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca
     4501 ggcggtattg gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta
     4561 gtcagccaaa tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga
     4621 gaagggcgga tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt
     4681 gcaaaaaaat atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa
     4741 ggactttctg attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa
     4801 gaagcaatgg aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc
     4861 agatttttca ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat
     4921 ttcaaatata ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag
     4981 ctgcattcag actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc
     5041 ttggtttttt cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc
     5101 agttctgaaa ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa
     5161 taaaaaatct gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc
     5221 taaattttga cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa
     5281 ctttactgaa tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag
     5341 aatttgccta tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa
     5401 atgatattac ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag
     5461 atcctgaaat cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc
     5521 tactggcact tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc
     5581 atttggatat ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct
     5641 tcatcgtggt cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa
     5701 tcgaaaaaag tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa
     5761 aacttcgtga tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc
     5821 taaagaaaac agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa
     5881 agaaacaagt cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg
     5941 ctgatgctgc acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga
     6001 tcagcgagaa agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata
     6061 gccaagcgtc tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa
     6121 atctgttatt cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag
     6181 gtcctaacgg tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca
     6241 aggttatagg agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt
     6301 atgaagacaa tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag
     6361 catttttgaa caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg
     6421 agcagatgag tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag
     6481 ctgaactata tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat
     6541 tagaacaact gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct
     6601 tcctggataa agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc
     6661 aagaacaaca aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt
     6721 atgtaacgag acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag
     6781 atgtgccgct gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag
     6841 atttgcctgt tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag
     6901 cacaagcagt tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa
     6961 tggattttgg ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt
     7021 tagcgttcgc tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg
     7081 ttcctttatt gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata
     7141 atgccttgat aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta
     7201 cattcatggt agataataca gaattggttt cttatgagta attatgtttt tgtgttattt
     7261 aaataaaata tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat
     7321 tcttttgata atataaactc aacctttaga aatgaaattt aattatttta ataaaagtat
     7381 tgaaattttg tttctttaga tatatactct agtcatagaa atgaagacgg atacagaaaa
     7441 agagggataa tatgtttggc ttttcaatat ttatgaacga ttctttgtca gaagagaaga
     7501 aacagtatat ccgccaaatg gcatccaatg gctttgtcgg tattttcact tccatgcata
     7561 taccagaaga tgatgtttcg gcatatcaaa agcgattgat cgaactagga agcgaagcga
     7621 aaaagaataa actagaattg atggtcgata tttcaggaga ggcattggaa cgagcgggtt
     7681 tttcattaga agatctaacg cctttgaaag caattggtgt aacaggttta agaatggatt
     7741 atcatatttc caatcagcag attgccgact ggtcacatca attgaaaatc agtttgaatg
     7801 ctagcacgat cacgcctaaa gatatcgatg aattgaaaga agcagaagct gatttctcac
     7861 agattgaagc ttggcataat tattatccac gtcccgaaac agggctggac aaagaatggt
     7921 atcagaaaaa aaatcaatgg ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag
     7981 gagatacaga gttgagaggc ccactatatc aaggattgcc aactttggaa gaacatcgag
     8041 ggatccatcc tcttgcaggc gctctagatc tattagcaag taacacggat attgtttaca
     8101 tcggagatgc aggtctgtca gaaaacgttc aggagcaatt tgcttctttc caaaaagaac
     8161 agacagtctt gctccatacg gaacctgtag atgaagaatt ttacgaatat atattaggga
     8221 agcatacgaa tcggcaagac gatgcaagag atgtcatacg aagcgtagat gcacgttttc
     8281 gagagattcc gcctatccca gcaagaaata cagctacgcg tatgaaaggg tctattactt
     8341 tagataacga aaagtatttg cgctatatgg gtgagatcca attaaccaaa tatgatttgc
     8401 cagcggatga aaaagtgaat gtagtagcta aagttatcaa agaagagcta ccattgatca
     8461 accaaatcaa agctggtatg aactatcaat ttataagaaa agaaggaaga taaaaatgag
     8521 caaaatcaat ttagagaacc taacaacgga acgcagaaac caagaaactt ttggattaga
     8581 tgagatgagt atttctcaag ccgttcaact aatgaacaaa gaagatcaga aagtggccgc
     8641 tgcggttgaa aaagaactga caagtatcga accagtgatt gctaacacga ttgagtcctt
     8701 taaaaaaggt ggacgtctga tttatatggg agccggaaca agtggaagac taggtgtgtt
     8761 agatgctgct gaatgcgtac cgacattcgg tgtagaaccg gaaatggtca taggtctaat
     8821 tgctggtgga gaagaagcaa tgactgtagc tgtcgaaggg gccgaagatt cttatgagct
     8881 agggcaagaa gatctaatca agctgaattt gacaaaaaat gatatagtta ttggtatcgc
     8941 agctagcggt cgtacaccat atgtgatcgg tggattagat tacgcaaaat cgatcggtgc
     9001 tgtcacagga acgatttctt gcaataaaca agccgaaatc agtaaacacg cggatttccc
     9061 aatcgaagtc gatgcaggtc cagaattttt gacaggctct actcgattga agtcaggaac
     9121 agcacaaaaa cttatcttga atatgatttc tactatcagt atgattggta taggtaaagt
     9181 attcaataac ttgatggtcg atgtcaaacc aacaaatgaa aaactaatcg aacgctcaaa
     9241 acgaatcatc atgcaagcga cagaagctga ttacgaaaca gctgaaaagt actttgaaga
     9301 agcagatcaa aatgttaagc tggctatcgt catgattttg acaaatagtg ataaagagga
     9361 agctcaagca aaattagctg aagcaaacgg atttgtaaaa gggaccttat gagggggaaa
     9421 agaccatggc aaaaaacgaa ctgactttgg aagaactggc aaaacaaatt tataaagggg
     9481 caggcggcat gggcaatgta gaaagcattg tccactgcat gactcgtgtg cgtatgaaga
     9541 tcatcaatga tgatcttgta aatgtctctg aattaaaagc gattccagga gtgcttggtg
     9601 tagtagaaga tgaacagctt caagtcatca tcggacctgg gaaagtgaac aaagtagcac
     9661 agcaaatggt tgatttggca ggcgtgaaat taggagaaaa actttcttct gtaagtacag
     9721 ctacagcaaa ttcatcagca tctggaaaag accgtgtaaa cgaacgggca caagaaatga
     9781 aagcggcaca aaaatcgaaa caaaaaccat ctaaaatcaa atcggtatta aaagacattt
     9841 ctaatatctt tgtgccaatg attcctgcat ttgtcggtac tgggatcgtg gcagggattg
     9901 ccgcagtttt agcaaactta gtcacagccg gaacacttga tgcagcaaca tggcaacaat
     9961 atatcgatgt gatgaacatc ttgaaaaacg ggatgtttag ctacctagtt atctatacag
    10021 gtatcaatgc tgcacaagtt tttggtgcaa caccaactct tggtggagtt atcggggccg
    10081 ttgtgatgct gacagggatg aatccggaag caccactgaa aaacttattt actggtgaag
    10141 ctcttgcagc tggacaaggt gggatcctag gtgtcatctt cgctgtttgg ctattgtcaa
    10201 tcgttgagaa aaaactgcat aaagtagtac ctgatgcggt agatatcatc gttacaccaa
    10261 ctctttcatt gattgccatc ggtctgattg aaattttctt catcatgcct ttagcaggtt
    10321 tcatttctga tggcatggtc ggtgggatca actgggtact tggcgttggt ggagcatttg
    10381 ccggcttcgt tctaggcaca ttattcttgc cgatggttat gttcggactg catcaaattt
    10441 tgactccaat ccatgtgcaa atgattgatg aaactggtcg tacgctattg cttccaattc
    10501 ttgcgatggc tggtgcagga caagtgggtg cagcattggc attatggatt cgctgcaaaa
    10561 aagataaaga attaacagaa atgatcaaag gagctttacc agtcgggatc ctaggaatcg
    10621 gcgaaccatt gatttacgga gtaacattac ctctaggacg cccatttatt actgcttgta
    10681 tcggtggagg tatcggcggg gcagtgatcg gaatgatcgg aaatgtcggg gctatcgcta
    10741 ttggaccttc aggagccgct ttgatcccat tgatctctga cggtaaatgg ttaggctaca
    10801 tccttggatt attggcagct tatgctggtg gcttcgttgc tacattcttc ttcggtattc
    10861 ctaaagaccg tttagaaaaa gaagaattag cgactgaaac agccacagta aatgaagtgc
    10921 ctgaaaaagt agtacctcaa acttcagctg aaagtgtgac tatcctctca tcagttgctg
    10981 acggaacagt tgtttcttta gataaagcaa gtgatccagt ttttgcacaa aagatgatgg
    11041 gtgaaggcta ctttgtagaa ccagaaaacg gacatatcta ttctccagtt actggggtag
    11101 tcagctctgt cttcccaacg aaacacgcta ttgggatcac aacgccaaat ggtttagaaa
    11161 tcttgcttca tatgggaatc aacacagttg acttaggcgg tacgccattt gatgtgaaag
    11221 taacagaagg gcagcaagtg acacctgata cgcttgtagc agaagctgac ttagaagcga
    11281 tcaaagcagc aggcaaagaa acatccatga tcgtcttggt aaccaatatg gatcgtgtaa
    11341 aaaactttgt cttggaaaaa acaggtaaag caaaagcaaa agcacctgtg atggatgtca
    11401 aagcttaata tgatagaaac aatagaaacg aaagaagaaa ctgaaatcta tttttcagct
    11461 tcttcttttt tattatttat tgttttctgc taaaatctga taaattcgtg tcacatcaga
    11521 tggtgttccc cgtaattcat aatctgccaa gaaaggaaag ccaaatgttt gtgcatattg
    11581 tttggctgtt agacaatact gattattgaa gtttttattt ccgctaccga ctacaccaag
    11641 acaaagttta tgattgtctt catgttccag atattcacgc atcgtttcgg tcaagatttc
    11701 tgtatcaccg ttatctaaac catttccgcc gtctaaataa gtaggaacga aagtaaaaaa
    11761 tggttctgtt tctttcgtaa aatcactatt ttcatggatt tcttttaaag taacttcagg
    11821 attgacagga tcttctgcat gcattttttc agcatattct gctagatgtt tggcaaaggc
    11881 acgcgtattt cctgaaatcg atatatataa aatgttcatg gatcatcctc caattctttc
    11941 cttttagtat agcatctttt ttaagttttt tggtggtgta atttggaaag ggactaggcg
    12001 ctttgcttgt ataatgagaa aaaagccggt agaattatat ttctattaaa gtttttatta
    12061 attaagtaaa aataaaaaag gtttgttata ctttttgata aaagcggttt tattttggag
    12121 gatgcttaca tggatacaga gcaacagaaa aaaggaattg gtttaattcc acttgctgcc
    12181 ttagtaattg gttcagcgat tggaggtggc gtatttggta ttatgacgga catctccgga
    12241 tcggctggcg gcccagctat tttcagttgg g
//
LOCUS       DACEMO010000395         9769 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.guided.102, whole genome shotgun sequence.
ACCESSION   DACEMO010000395 DACEMO010000000
VERSION     DACEMO010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.guided.102"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1823
                     /locus_tag="IW728_002868"
     CDS             <1..1823
                     /locus_tag="IW728_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1026183.1"
                     /translation="ISFLVHEKDRIGLIGTNGTGKTSLLNIIAGIDSSDGDRQTVFYP
                     TDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRAYEEALIELGENGEDPDVQKRY
                     AKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKISTLSGGQKKRVSLAQVLIDEPDL
                     LLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRYFLDRVANRIFELSFGKLYEYK
                     GNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRAGVQARGTKQQARINRFEDLKE
                     NLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQTLLEHLDLLIQSRERLGITGK
                     NGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQENEEMAPDKRMIAYLQEAAEEA
                     KTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKRRLFLLKLLIQQPNVLLLDEPT
                     NDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVADKLLVFQGNGKQELYYGNMSSY
                     LLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEWETIEDEIAELEEKISLLQEEM
                     NHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEWVED"
     gene            1835..2782
                     /locus_tag="IW728_002869"
     CDS             1835..2782
                     /locus_tag="IW728_002869"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1026184.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2802..3329
                     /locus_tag="IW728_002870"
     CDS             2802..3329
                     /locus_tag="IW728_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1026185.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3392..4045)
                     /locus_tag="IW728_002871"
     CDS             complement(3392..4045)
                     /locus_tag="IW728_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1026186.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4177..5019
                     /locus_tag="IW728_002872"
     CDS             4177..5019
                     /locus_tag="IW728_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1026187.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5177..6073
                     /locus_tag="IW728_002873"
     CDS             5177..6073
                     /locus_tag="IW728_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1026188.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            6076..6789
                     /locus_tag="IW728_002874"
     CDS             6076..6789
                     /locus_tag="IW728_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1026189.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            6809..7327
                     /gene="msrA"
                     /locus_tag="IW728_002875"
     CDS             6809..7327
                     /gene="msrA"
                     /locus_tag="IW728_002875"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1026190.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            7324..7551
                     /locus_tag="IW728_002876"
     CDS             7324..7551
                     /locus_tag="IW728_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1026191.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            7703..8254
                     /gene="lepB"
                     /locus_tag="IW728_002877"
     CDS             7703..8254
                     /gene="lepB"
                     /locus_tag="IW728_002877"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1026192.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8337..8879)
                     /locus_tag="IW728_002878"
     CDS             complement(8337..8879)
                     /locus_tag="IW728_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1026193.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(9116..9514)
                     /locus_tag="IW728_002879"
     CDS             complement(9116..9514)
                     /locus_tag="IW728_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1026194.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         3508 a         1698 c         1924 g         2639 t
ORIGIN      
        1 agatttcttt tttggtccac gaaaaagacc gtatcgggtt gattgggacg aacgggacgg
       61 gtaaaacaag tctcttaaat attattgccg gtatcgatag cagcgacggt gatcggcaaa
      121 cagtgtttta tccaactgat taccgtattg gttatctttc tcaagcagct gaattttccg
      181 atgaattaac tgttctccaa gcagtctttc aaggaaacag ccccttgatc caaactgttc
      241 gagcatatga ggaagcattg atcgaactag gggaaaacgg agaagaccct gatgtgcaaa
      301 aacgttatgc taaagcagaa gaacaaatga ataaagaaga tgcttggact acagatacca
      361 acgcaaagat tattttgcaa aaattaggaa tcagtgaatt agataaaaaa atcagtactt
      421 tatcaggtgg tcagaaaaaa cgagttagtc tggcgcaagt gttgattgat gaacccgatt
      481 tactattact agacgaacca acaaaccatt tagattatga agcgatcgag tggttggaaa
      541 actatttgaa acaataccgt ggcgcactat tgatggttac ccacgatcgt tattttctag
      601 atagagtagc aaaccgaatc ttcgaacttt catttggaaa attatacgag tataaaggaa
      661 attatgaagc ttacgtctta gaaaaagcag aacgtgatcg tgtagaagta gaacaagaag
      721 aaaagcgcaa gcggctttat aaacaggaat tagcttggat gagggctggc gtacaggcac
      781 gtggcaccaa gcagcaagca cggatcaacc gtttcgaaga tctaaaggag aacttgtacc
      841 aagtaaacca agaagatgat ttagagctga acctagcgac acaacgttta gggaaaaaag
      901 tactggaaat caaagatggc agttaccgta taaatgacca aacactgcta gaacatttag
      961 atttattgat tcaatctcgg gaaagactag gtattactgg taaaaatggt gcgggaaaat
     1021 cgacattgct taatattcta gctggcagaa ttcccctgga tagcggtaca atgtctatcg
     1081 gtgaaacggt tcatcttgcg tattatacac aagaaaatga agaaatggca ccagataaac
     1141 ggatgattgc ttacttacaa gaagctgctg aagaagcaaa aacagcagat ggcagtcaaa
     1201 tcggagtagc agaattactt gaacgatttt tatttccaag atttatgcat gggacactga
     1261 taggcaaact ttcaggcgga gaaaaacgcc ggttattttt gttgaaactg ctgattcagc
     1321 aacctaatgt tttattattg gatgaaccga ccaatgattt ggacatagca acattgacga
     1381 ttttggaaga ttattttaga agttttccag gtgctgtgat cactgtttcc catgaccgtt
     1441 atttcttgga caaggtagca gataagttat tagtattcca aggaaacgga aaacaagaac
     1501 tttattacgg aaacatgtcc agttatttac tgaagcaaaa agagacccag cagcctgctg
     1561 aaaaagcgaa accaaaaata cagagcaagg aacctgctga aaagaagaaa ttatcttaca
     1621 tggaacaaaa agaatgggaa acgattgaag atgagattgc agaacttgaa gaaaaaatct
     1681 cattgctcca agaagagatg aatcatcaag gagataattt cactcgatta caagaattgc
     1741 aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa aatggcacgg tgggaatact
     1801 tgagtgaatg ggtagaagat taggaggaaa aaggatggaa caagcgtatc ttgacttagg
     1861 aagaaaattg ttggaagaag ggcatcgtaa agaagacaga acaggcacag gcacattaag
     1921 cttattcgga taccaaatgc ggtttgatct gcaaaaaggg tttcctttgt taacaacgaa
     1981 acgagtacct tttggattga tcaaaagcga attgctttgg tttttaaaag gagatacgaa
     2041 tatccgctat ctattacaaa ataaaaacca tatttgggat gaatgggcat ttgaacgcta
     2101 catcaaaagt ccagactata atggaccaga tatgactgat tttggccata gatgtttgac
     2161 agatcctgaa ttcaatgaag aatataaaaa gcagagcaag cttttttgcg agaagatcct
     2221 aaccgatgat tcctttgctg aaacatacgg cgatcttgga cacatctacg gctatcaatg
     2281 gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa atcaaacaag tgatcgaagc
     2341 aatcaaaaag actcctgatt ctagaagatt gatcgtttct gcatggaatc cggaagatgt
     2401 tcctagcatg gctttgcctc cttgtcatac gatgtttcaa ttttatgttc aagagggacg
     2461 gctaagctgc caattgtatc aacgcagtgg ggatgtgttt ctaggcgttc cgttcaacat
     2521 tgcaagttat gcgttgctga ctcacctaat tgcacatgaa acaggtttag cggttggcga
     2581 atttgttcac acattaggag atgctcatct ttatatcaat catatcgaac aaatgaaaga
     2641 acaattgagc cgagaaatac gcgcatttcc gactttagtt ttgaatcagg aaaagcagtc
     2701 agtatttgat tttgagatgg aagatatcca attagaagga tatgacccac atccggcgat
     2761 caaagctcct atcgctgttt agaatcagaa aggatgagaa aatgttagct gcaatttggg
     2821 cacaggatga aaacggattg attggaaaag aagaccgtct tccttggcgt ttgccaaatg
     2881 atctgaaatt cttcaaacaa atgacagaag caaatacact agtgatggga cgaaaaacat
     2941 tcgaagggat gggtagccgt ccgttgccga atcgtcagac gattgttttg acgagagatt
     3001 cttcctataa agcagaaggc gtgcatgtca tgcatagtgt ggaagacgtg cttgctctgg
     3061 aaaaagaaac agacggtatc tttttcatcg ctggagggtc agctgtttat caagaatttc
     3121 tgccattttg tacgatatta taccgtacag tcatccatca tgtattcgat ggagatgctt
     3181 attttccacc tgttgactgg tctgattggt cactgatcaa tactagtcaa ggagaaatgg
     3241 atgaaaaaaa tcgttatccg catcaatttg aaacgtatca gcgtaatgag aatcctgtaa
     3301 aaagcgggac aggcgaattg acgatataaa aaagctatgg atagactagc tgttatttag
     3361 agctgtccca tccatagctt tatttttttt attaagtaaa gaataaaaca gagaagtaca
     3421 taaaacctgc acctaacata acaaacaaat gccaaacaac atgcatatag cggatagaat
     3481 tcaagctata aaagaatgcg cctatcgtgt aagaaagccc gcctgctacc atcaaaataa
     3541 caccagtcat tccaagagag tgataaagag gaacagcagc cacgacacag agccacccca
     3601 taaaaatata gatgatcgta gatacttttg atactctctc ccgtttatgc aacgtcaacg
     3661 ctttatatat aatgccaaga atcgcaaaca accagattaa tacaaataaa gtccaaccta
     3721 gccatcctcg gatactcagc aaacaaaaag gagtataact tcctgcaatc aaaagaaaaa
     3781 tcgaactatg atcgaatact tggaacacct tttttgcttt tgtaaaaatc aatgaatgaa
     3841 acaaagtaga cgttaaaaat aaaagaatca tcatcgatcc atagatagca taagagacaa
     3901 tatggaggga ggaatgcaag tgggcacctt ttactaaaag aaccactagt cccgcgatac
     3961 tcaatccagt cccaattcca tgggtgactg cattaaacac ttcatttaat atttcatacg
     4021 ttcgattaaa tttcgttttt tccatgaaaa cggctccttt actaggtgta attaatgaaa
     4081 aatcattatt tgtctgttat actaaaaata gaaattgtct tacatgtagt ttaacatgaa
     4141 taaggcatct tgttaaagaa agagagagag aaaataatga caaatgtaaa aatcgtaaca
     4201 gattcttcat gtacgatgga aaaaaatgta cgcgatgctt taggcattcg tgtcatgccg
     4261 ctatccatca tgatcgacgg aacagtatat cccgatgatg accaactaga cggcgaaaga
     4321 tttatggaaa tgatggcgaa agcaaaaaat ctgcccaaaa caagccaacc accaattgga
     4381 gaattcatct cgatgtatga tgagctagga gaagacggca gtgaagtgat ttcgatccat
     4441 atgacaaaag gcttgagtgg aacagtagaa gcagcaagac aagccagtaa tctaacaaaa
     4501 acgaaagtaa cggttatcga cagtgatacc actgaccaag gattatcttt tcaggtaatt
     4561 cgagcagctg aattagcaaa agaaggtaaa acagtagaga aaatccttga agaagtcaaa
     4621 aaagtacaag acaatacaaa attatacatc ggaatatcca cactagataa cttggtaaaa
     4681 ggtggaagaa tcagccgaac aactggtttg ctgtctagtg ttttgaacat acgcgtggtg
     4741 atgaatttcg cccattctga attaatccct gtaactaagg ggcgaggaaa gaaaacgttc
     4801 gataaatggt ttaataattt gaaaaatgaa ctgaaagaat tgccaaatgt gcgacaaatc
     4861 ggtatttcac atgcggatgc aaaagaattg gcgcaagaat tcaaagaggg tttgcaagaa
     4921 ttattcccgg acatgcatat ccctgtactg catacgaatc cggttatcgc tacgcatact
     4981 ggtaaagacg cgtttgcaat catgtattat gtagattagt cgaaaagagg ataagccctc
     5041 agcctgaaag gcttgcagct tattctcttt ttctttataa aattaggaaa ataattgttt
     5101 tcttttttca taaagactac ctttttgata aaatttttga taacattaga accaatcgaa
     5161 tacaagaggt gaaaaaatga aattaacgaa acgacatttg atcctcgttt tgttcccatt
     5221 attaattgcg atcatcatct atgcaggttt agctgcttct ataccgaaag ctggcccact
     5281 aatgaaacca gtaactactg tgagagaaga tccaaataaa aaagaaaaac ttcactacat
     5341 agctatcgga gattcgctga ctgaaggagt aggagatcaa accaagcaag gaggattcgt
     5401 tccccttgtc gcaaatgatc taaaagaccg ttataatttg accgcggttg aaacagaaaa
     5461 ctatggtgta aatggtgaac gaagtgacca gatcttaaag cgagtaaaaa caaaagaaga
     5521 aattcgaaac aatatcaaaa cagctgattt tatcacactg actgttggcg gtaatgattt
     5581 gatgaaagtt atccaaaata atttattcgg gttaacggtc aattccttta aaaagccact
     5641 gaaaaactat caggaaaatg tcacagaact attggaagaa tttcgatccc tcaataaaac
     5701 cgcacctatt tacattttag gcatctacaa tccattttat ttgaactttc ctgagatcac
     5761 tgacatgcag acaattgtta acaattggaa caaaggaaca gaaacggtga caaaaaaaat
     5821 gccgaactgc tactttatcc caatcaatga tttgctttat caaggattga atcaggaagt
     5881 aggtgtcaca gaagaaggaa cagatcaaac aactagcacg acagaaaatc taaatggcat
     5941 caaaaataat gtgctctatg aagaagatca tttccatcca aataatttag gttatcaatt
     6001 gatggcaaat gcagtgagag atcagctgat cgagacacaa aaccaatggt tagagaagga
     6061 ggatgatcaa taaacatgaa aacaagatct gacgtatctt caaacaaaac aaagaaaaca
     6121 aattactgga aattcgcctt ccttatcctt cttggggtag tacttggttc gaccgtattt
     6181 ttaggcagcc gtatttttgc taatcgggaa ccagaactcc cagaaatacc agctcttacg
     6241 gaaagacaag gagatcctgt cttaacgatc aattcaaata aagaaaaagt caatcaaatc
     6301 atcagctttt tccttagtga gtatcaaaaa gatagtgata ttgaatataa attttatttg
     6361 gaaaatgaag cactactcaa tggtactttc gaagtactag gttttcctat cgatttctat
     6421 ttatattttg atccgtatgt gatggaaaac ggaaatgtcc aactaaaagc tagaagtctt
     6481 tctatcggaa cattgagttt gccaatcaaa gatgtgatga atatgatcaa gagaaactat
     6541 aaattaccag attggatcga agtcaataca gatgatctga ctatcatgct taggttggat
     6601 caattccgaa tgcaaaacgg gatgtatatt aaagcagata aaatcgactt agtaaatgat
     6661 gacattcggt ttagtctata tttgccaaaa gatacctctg atgcttcaaa caactcaaat
     6721 accaacaaaa ctagcaccag cagtactgaa agcaagacaa aagaaaataa aaaatcatcg
     6781 gatgagtagt attagagggg gaatcataat ggaacgagca atatttgcag gaggctgctt
     6841 ctggtgtatg atccagcctt tcgacacgct tcctggtatc catacagtga tgtcaggata
     6901 tactggaggg catgtaccag atccgactta tgaacaagta aaaagccaaa cgactggaca
     6961 tacagaagca gtggagatta tctatgatcc aacattgatt tcatatgaac aattagtaga
     7021 actttactgg cagcaaactg atccaactga cgctttcgga caattcgagg atcgcggtga
     7081 caattatcgc ccggtgatct tttatttgac agaagaacag cggatcattg ctgaaaaaag
     7141 taagaaacag ttagctgaaa gtggacgttt cgaacagcca atcgttacaa aaatcgaacc
     7201 agcccaacca ttttatctag cagaagaaga ccatcaagag tattataaaa agcatcctga
     7261 aaattttaaa agaaaccatg cacgccgggc tgcctttatc gaagaaaatt ggaggaacga
     7321 agggtgaatc gaagcttttt tcattattta atgacactgc gaggcggtgg cacacatgat
     7381 gctttatctg ttttcgctac agaagccggt aaagacatcc aatttcctaa gcacagctct
     7441 agctacgaag aaatttctaa ttatctggaa ctgaatgtcg actatttgcc gtccatggat
     7501 atctttgacg aagcatggga aaaatattta gaaaataatc atgacatata agagatagtt
     7561 ttgtacttta tgatcaaaaa agttttttgt ccaatagcct aattgaaaga attattttta
     7621 tttcagctat ctagtcgcat ttttttgata tttttcttat aataaagaaa gaatgaaaaa
     7681 aagagggatg gattgataga gattgacaaa aaaacaacgc tatattaatc gcttctggct
     7741 tgtattcaaa tacttacttg tttctgtatt ccttgcgttc atgttgcggg gatttttatt
     7801 catcccggta ccagtggaag gcaattcaat ggaaaacgtg ttgaagcaag gtgatatggt
     7861 tgtgatggaa aaattttcgg agatcagacg atttgatatt gttgtttttc aattagcaga
     7921 tggcacgatt tatatcaaac gagtaatcgg tcttccagga gaaaatgtca gctaccaaaa
     7981 tgatcaattg aagatcaatg ggaaagtagt taaggaacct tatctaacta aaaacctgaa
     8041 atcagatcat gcaaatgcat cctatacgac ggactttaca ctacaagagc taacgggaca
     8101 atccaagctt ccggaagata attattttgt acttggagat aatcgaagag tttcaaaaga
     8161 tagtcgttcg tttggcacga tcaataaaac tgatattcta ggtaaagcaa gatttgtcta
     8221 ttatccactt gatgaaataa aatggatcca gtgagaaaca ataaagcgtg cagtctgttg
     8281 agactgcacg ctttattgtt taatccaaaa tcagtttact accttagtac gtaaaatcaa
     8341 acagctcgtt taaacacttt tctagcctct aaagttttga taaatgaata aacaaccatt
     8401 atttgaatag ggatttggat catattcctg atgaatctac ctagaaaaat tactttgaaa
     8461 tctgcaccaa acaaaacaga catcagccaa gaattgataa aaaggctgac tgggatcata
     8521 atcaataatt gggcagcgat gacggaaggc aggtttatct ttttatgata taaaaataag
     8581 ccattaataa atccaccaag gaaagccgtg ataagaaaag cagcttgaaa cgttcctgaa
     8641 ccaaatatcg tgaaatttaa agtgttcgac aacatttcaa gcaaaccagc ccagatcggt
     8701 ccatatagat aacccataag cgccatactt aaaaatccga atgtgatttg aaaataagca
     8761 ggaccaatcg ctatcttgta taagatgaca tcaagggcta ataaaagagc agaggtcgac
     8821 aacaatcttg cattcatttc gggcaatcta aaaacgtctt ttgcaatcaa agtgttcaaa
     8881 atattctctc ctttgtcagt taataataaa aggataccat aaaatcacca atgtgtcatg
     8941 acacattggt gatttacata cactggcatc taacaattaa aaaaagggtg agaagactgc
     9001 ttttataaac tgtaaaaaag agattgtacg aataaaaaaa atagaggcgg agaatactta
     9061 tcgtatcatc cgcctttgtt tttttgttat aaaaaacgtc agattagtta tctgcttaca
     9121 tcttcatcca tacgggttct aatgtatttc cgtctagatc ttggacttct aatgaaaaca
     9181 tttcttcttc aggcattccc atatctactt cgtggaaagt accaccattt tcttttgcaa
     9241 tttgagcaaa tttttttact tcatccgcat tttcaacact aaacgaaatc aaagcaccac
     9301 ttgttttttt attatcagct atttctttat cttttataaa ttttttataa aaatcatgag
     9361 agagaagcat tatccaaaaa gtgtcatccc ataccattga accagcttct tcagtagaaa
     9421 attcttcatt ttttttaaaa cccagctttt cataaaaagc aacggaacga ttgacatcag
     9481 caacaggaaa attaacaaaa accatactat tcatttgttt cgtctccttt ctgttttgtt
     9541 cgtctatcat ctcttattta ggaaacgctg tcaaaaattt agattcatat atagaaacca
     9601 aaataaaaat ttgcatcatc aaaaaagtga agatgtgata gaatgaggac tggttcactt
     9661 tcacttaact aatagatgga atttctttat aataaaaaag tgaatttata ttgaaaaaga
     9721 aaaaaacaag taaagccggg gaatattccg gtatgaaagg aatggagag
//
LOCUS       DACEMO010000396         7851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.guided.128, whole genome shotgun sequence.
ACCESSION   DACEMO010000396 DACEMO010000000
VERSION     DACEMO010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.guided.128"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1564
                     /locus_tag="IW728_002880"
     CDS             128..1564
                     /locus_tag="IW728_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1026195.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IW728_002881"
     CDS             1753..4200
                     /locus_tag="IW728_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1026196.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IW728_002882"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IW728_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1026197.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IW728_002883"
     CDS             complement(5690..6793)
                     /locus_tag="IW728_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1026198.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..>7851
                     /gene="aac(6')"
                     /locus_tag="IW728_002884"
     CDS             7346..>7851
                     /gene="aac(6')"
                     /locus_tag="IW728_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1026199.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPD"
BASE COUNT         2618 a         1344 c         1710 g         2179 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata t
//
LOCUS       DACEMO010000397         7780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.guided.130, whole genome shotgun sequence.
ACCESSION   DACEMO010000397 DACEMO010000000
VERSION     DACEMO010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.guided.130"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1213
                     /locus_tag="IW728_002885"
     CDS             245..1213
                     /locus_tag="IW728_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1026200.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IW728_002886"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IW728_002886"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1026201.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IW728_002887"
     CDS             2243..2851
                     /locus_tag="IW728_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1026202.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3279..4190
                     /gene="vanY"
                     /locus_tag="IW728_002888"
     CDS             3279..4190
                     /gene="vanY"
                     /locus_tag="IW728_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ1026203.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4343..4828
                     /gene="vanZ"
                     /locus_tag="IW728_002889"
     CDS             4343..4828
                     /gene="vanZ"
                     /locus_tag="IW728_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1026204.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5115..5444)
                     /locus_tag="IW728_002890"
     CDS             complement(5115..5444)
                     /locus_tag="IW728_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1026205.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5749..6078
                     /locus_tag="IW728_002891"
     CDS             5749..6078
                     /locus_tag="IW728_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1026206.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6068..6355
                     /locus_tag="IW728_002892"
     CDS             6068..6355
                     /locus_tag="IW728_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1026207.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(6634..7197)
                     /locus_tag="IW728_002893"
     CDS             complement(6634..7197)
                     /locus_tag="IW728_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1026208.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2566 a         1297 c         1546 g         2371 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat
     3301 tgttattgtt attcttaata tacttaggtt atgactacgt taatgaagca ctgttttctc
     3361 aggaaaaagt cgaatttcaa aattatgatc aaaatcccaa agaacattta gaaaatagtg
     3421 ggacttctga aaatacccaa gagaaaacaa ttacagaaga acaggtttat caaggaaatc
     3481 tgctattaat caatagtaaa tatcctgttc gccaagaaag tgtgaagtca gatatcgtga
     3541 atttatctaa acatgacgaa ttaataaatg gatacgggtt gcttgatagt aatatttata
     3601 tgtcaaaaga aatagcacaa aaattttcag agatggtcaa tgatgctgta aagggtggcg
     3661 ttagtcattt tattattaat agtggctatc gagactttga tgagcaaagt gtgctttacc
     3721 aagaaatggg ggctgagtat gccttaccag caggttatag tgagcataat tcaggtttat
     3781 cactagatgt aggatcaagc ttgacgaaaa tggaacgagc ccctgaagga aagtggatag
     3841 aagaaaatgc ttggaaatac gggttcattt tacgttatcc agaggacaaa acagagttaa
     3901 caggaattca atatgaacta tggcatattc gctatgttgg tttaccacat agtgcgatta
     3961 tgaaagaaaa gaatttcgtt ctcgaggaat atatggatta cctaaaagaa gaaaaaacca
     4021 tttctgttag tgtaaatggg gaaaaatatg agatctttta ttatcctgtt actaaaaata
     4081 ccaccattca tgtgccgact aatcttcgtt atgagatatc aggaaacaat atagacggtg
     4141 taattgtgac agtgtttccc ggatcaacac atactaattc aaggaggtaa ggatggcgga
     4201 atgaaaccaa cgaaattaat gaacagcatt attgtactag cacttttggg gtaacgttag
     4261 ctttttaatt taaaacccac gttaactagg acattgctat actaatgata caacttaaac
     4321 aaaagaatta gaggaaatta tattgggaaa aatattatct agaggattgc tagctttata
     4381 tttagtgaca ctaatctggt tagtgttatt caaattacaa tacaatattt tatcagtatt
     4441 taattatcat caaagaagtc ttaacttgac tccatttact gctactggga atttcagaga
     4501 gatgatagat aatgttataa tctttattcc atttggcttg cttttgaatg tcaattttaa
     4561 agaaatcgga tttttaccta agtttgcttt tgtactggtt ttaagtctta cttttgaaat
     4621 aattcaattt atcttcgcta ttggagcgac agacataaca gatgtaatta caaatactgt
     4681 tggaggcttt cttggactga aattatatgg tttaagcaat aagcatatga atcaaaaaaa
     4741 attagacaga gttattattt ttgtaggtat acttttgctc gtattattgc tcgtttaccg
     4801 tacccattta agaataaatt acgtgtaaga tgtctaaatc aagcaatctg atctttcata
     4861 cacataaaga tattgaatga attggattag atggaaaacg ggatgtgggg aaactcgccc
     4921 gtaggtgtga agtgagggga aaaccggtga taaagtaaaa agcttaccta acactatagt
     4981 aacaaagaaa gcccaattat caattttagt gctgaggaat tggtctcttt aataaatttc
     5041 cttaacgttg taaatccgca ttttcctgac ggtaccccca aactaagatt tcatagatag
     5101 attttctgat aactttaaaa aacacgaata gcggaaagga tgtcttcatt cgctaaagag
     5161 taataaacta cttttccatc ttgacgtgat tttgccattc catgtttttt taaaaaacgt
     5221 aaatggtgtg acgttgtagc gacactagca gtaagtattt ctgctaaatc acagacgcac
     5281 atttccttat aggtttctaa agcatagaaa attttaattc tagaaaaatc gctgaaacat
     5341 ttccctaaca caagtaagtt tgaaaaatct ttttgatcca atttattttt tattccattt
     5401 actttttctt tatgtacgac agtcacttgg caaacttttt tcattcatct cacctcacat
     5461 tcaaacgatt atttgaatga agtataccta tttgatcact cttttgtcaa tctaccttaa
     5521 ttccaataca ctattataag caatttcatg tattgaagta aacttacttg caagtgcgtt
     5581 tttgattcca agttaaaaac atattattat tctacataat ttcctaatca aataaaactc
     5641 ctttgaaaat tgttctaaag gagttttatt attttgtatt tctactaaaa atagcgtaca
     5701 atgtaattac taagtaatta cgaaatgtaa aataatggag gaaataatat gattacaaaa
     5761 acaagaaaac aaggaaattc tattatgtta acggtcccta aagaattcga tgtgcccaat
     5821 ggtgttgaag tagaagctaa gcttgtagag aatggtatcc tttatgagtt tgtagagcct
     5881 aaaaaagaat tttttgattt tagtgaagat attttggctg atattctttc agaaggttat
     5941 agcaaacaag agatcttaaa agaatttaga aatagaaaaa atgcgttaac ttctgcattt
     6001 aaatctattg ctgaagatac tgttgcaaat tctaaaccga tgacaaaaga ggagttggca
     6061 gcagaaattg gcttatgaga ttctaccttc taaacacgta ataaagtatc taaaaaaatt
     6121 aaaagagaaa acattaaaag aacaattttt aacgataatt tatgatgaga tagcagttcg
     6181 tcctcattcg ggagaacaaa aaacgggtga tttatcagga atttgggcta ttggattcaa
     6241 gtatgcaggt acgacatatc gcgttgcata tgaaattaaa gataatgcag tgatacctat
     6301 cttactatgt ggaacgcatg aaaattttta tgaacaatta aaaaagatta ggtaagaacc
     6361 caatgtttca acttgagagg atagacgaca ctttaaaaga taatttttaa cagctgagag
     6421 ttgtaatacc ctataatttt aaaccactaa cctatatatt tatgaaaaat agaaaaaatt
     6481 aaaacgcatt ttaagcctct ttcaaattaa aagcttcatc cttatgcaca aagagggctt
     6541 aaaatgcgtt attttatatt gttttttagt aataataaaa aaatcactct aattctccca
     6601 taaatattat agattcctaa agattaaaat agattattcc ctattgtctt ttaagtagcg
     6661 gtagagtgtt gccttgctaa taccagtcat ttccgtaatt tcttttacgc tataaacctg
     6721 acttttatac aattttactg ctcgatcaac atctttctgg ttcactcgtg gacggccacc
     6781 tttacgacct ctcgcacgtg cacttttcaa accttctttg gtacgttcaa caattaaatc
     6841 acgttcgaac tgactaaatg cttggaagac agtcatcatt aagcgtccgt gaggcgtgct
     6901 ggtatcgaag ttttctttca agctcaccag cttaacatgg tgttcttcaa aataggtaac
     6961 aaggtcaatc aaatcctttg tactacgacc caatcgagaa aaactttcta caattaccgt
     7021 atctcccgga cgaattttat cttttaaacg atttagttgg ggacgaaccg ctttggttcc
     7081 cgtcattttc tcagttagga tttcattaca attttgttca ttgagtaagt ctaattgacg
     7141 atttagttct tgtgaacgtg tactcactcg ggcataacca taaatgtatc cactcatgct
     7201 tttcttcctt tcgtacttta attctggctt gattgtatca taaaagggag ttatagatac
     7261 atagttaatg caacaagtta tgatacaaaa tatgacctct tttatttaat aaaagaggtc
     7321 atttcatttt gtctcataat cgattgtttt tgatacgagt gtgtatacga aataaacaaa
     7381 aaaatcttaa gtaaatttct agatacctaa tttaatccta actagtgtat cctaaagtta
     7441 ataacgagtt tatcacaatg aaaattataa aatgctccca tattagatat aacatgcaat
     7501 atttgaatta gaactacaaa atttattcgt tttataaagt aaataatgtt gagatactaa
     7561 ggaatagaat attataagga attgagctga atgtattgga taaaaaaaga ataactttat
     7621 tagagatgga ctataaaaat gctcgtaatt ttcttctgaa gtctgaaagc tatttgaatg
     7681 ctcagctacc agaatatatt agttttgacg aaactttaaa atctgctaaa aaagttttaa
     7741 cttcgccgtt tgtataatta agcgagacaa ataaaaaagc
//
LOCUS       DACEMO010000398         4613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.guided.179, whole genome shotgun sequence.
ACCESSION   DACEMO010000398 DACEMO010000000
VERSION     DACEMO010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.guided.179"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            109..192
                     /locus_tag="IW728_002894"
     CDS             109..192
                     /locus_tag="IW728_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1026209.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IW728_002895"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IW728_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1026210.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IW728_002896"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IW728_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IW728_002897"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IW728_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IW728_002898"
     CDS             2348..2593
                     /locus_tag="IW728_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1026211.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="IW728_002899"
     CDS             2696..3565
                     /locus_tag="IW728_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1026212.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="IW728_002900"
     CDS             3546..4280
                     /locus_tag="IW728_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1026213.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..4576
                     /locus_tag="IW728_002901"
                     /pseudo
     CDS             4313..4576
                     /locus_tag="IW728_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
BASE COUNT         1656 a          675 c          895 g         1387 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gtattcgaat
     4381 tgtgaccctt gaggggtcac gcgcaaatat taatatacct aaagatgaat ttcaggatta
     4441 tgatattaca tattttgtaa gtgatataga accgtttata tctaatgatg actggcttaa
     4501 tcaatttggg aatataataa tgatgcaaaa gccggaggat atggaattat tcccacctga
     4561 agaaaaggga ttttccggtt ctgttgcaaa gttttaaata aagaataaaa tcc
//
LOCUS       DACEMO010000399         3229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.guided.224, whole genome shotgun sequence.
ACCESSION   DACEMO010000399 DACEMO010000000
VERSION     DACEMO010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.guided.224"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            135..1349
                     /locus_tag="IW728_002902"
     CDS             135..1349
                     /locus_tag="IW728_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1026214.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1380..1598
                     /locus_tag="IW728_002903"
                     /pseudo
     CDS             1380..1598
                     /locus_tag="IW728_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2017..2490
                     /gene="dfrF"
                     /locus_tag="IW728_002904"
     CDS             2017..2490
                     /gene="dfrF"
                     /locus_tag="IW728_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1026215.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2617..>3229
                     /locus_tag="IW728_002905"
     CDS             2617..>3229
                     /locus_tag="IW728_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1026216.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIM"
BASE COUNT         1051 a          479 c          695 g         1004 t
ORIGIN      
        1 aaaaagagca caacacatca agcttttatt aattgtctgt cttttataaa tgcaagtatt
       61 aattcaaaaa aacagaagaa aaaaacaact acaccttctt ttatactcgt aatcgtagaa
      121 aggaggcgaa aaaaatgaat acattaccta tcaatacagg agatacagca ttcatgattt
      181 tatgtacagc aatggtttgc ctgatgactc ctggtctagc ttttttttac ggcggattag
      241 cacggaagaa taatatacta acgatcatgt ctcaatcttt tctctccgtt gggataacaa
      301 cgattatgtg ggtatttggt ggattcggat tggcctttgg acgggatatc ggcggtgtta
      361 ttggaaaccc ggcagatttc tttttgatga gacatgttac aagcttcccc aacatgtttc
      421 atggtgcaac gattccattt ttaatgttct ttctttacca actgatgttt atcgtgatta
      481 ccgttccttt gatgacagga gcttttgcag ggagattgaa tttaaaaggc tatattatac
      541 ttgttatttt ttggaatctg ctgatttatt tccctgtttg ccactggata tggggtggcg
      601 gatttttgga tcagatggga tttagagatt tcgctggtgg agcagtcatc cacacgacag
      661 ctggattcgg ctctttagca tgtatcctca cacttggtca aagaaagatt cctttaggag
      721 aaagaagtca gcatagcagt cttatggcag ctgctatcgg tacaggactg ctttggtttg
      781 gctggttcgg ttttaattcc ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat
      841 ttggttcaac attcatcgct cttgcatttg cgatgattac ttggctgatc attgcaaaag
      901 taaaaggaaa tggctttgat tttgtcgacg tactaacagg atccgttgct ggattggcag
      961 ccattacgcc ttgtgccggt tatgtagaag caaaaagtgc catgctgatt gggatcattg
     1021 cgggtatcgt ctgtcatgct gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac
     1081 ttgatgtgtg gggtgttcat ggcatgggcg gattcacagg tacgattctg atagggatct
     1141 ttgcatctaa cagccattta ctaacgaatc caagcagctg gtacttctta gggattcaag
     1201 tgattggtgt catcattact gccgtgtatg cctatgtgct gacaacagtg attttaaaat
     1261 cggcgatgca ctttacaacg atcactacta caaaagaaga acaagaagaa ggattggact
     1321 ttttcttctt tggcaacaaa ctcaattgaa acaaattcaa atagaaaaga ggaagattca
     1381 tgcaacaaag caaaacgaaa attcctgcaa aaaaattaac gttctttggc tttttggcta
     1441 tgaccatctc aatggtcgtt tcattatatg aatatcccac attcgcgacc tcaggcttca
     1501 gcttagtatt tttcttgtta ttaggcggtt tgctatggtt tatccctgtg gcattgtgcg
     1561 cagcagagat ggcaacggtg aaaggatggg aaaaaggtgt ttcccaaaca aaaagatatt
     1621 aagaaacagc ccatgtttac ctgcttgcaa acaaaagaat attgctacta gactaagaga
     1681 tactacataa gtgtgttggt atctcttttt tgttgccgtg aaacagcaga ctatttcatt
     1741 tctgcttgat taccttaaaa cttattagtc tatggtttgt caagggcttg ttgcgtgaga
     1801 aatgctgaat atcacccttt acaaaacata ggctgataag taacttttca aaagcagaaa
     1861 agaaatggca atgcttataa caatatgata taatgcagat gaagacaaaa aaggaatttg
     1921 tcgagctggt tagttcgaga taattttgag tttgttaaga tgtggtgcac ttgatggaat
     1981 cacaggtacg acatcatatt atggaggatg aaatagatga taggtttgat tgttgcgagg
     2041 tcaaagaata atgttatagg caagaatggt aatataccat ggaaaataaa gggagaacaa
     2101 aagcaattta gagagttaac aacgggtaat gtggttatta tggggcgaaa gtcttatgaa
     2161 gaaatcggtc atccgttgcc taatagaatg aatattgttg tttccaccac aacagagtat
     2221 caaggagata atttagtttc agttaaatca ttagaagatg cattattatt ggctaaagga
     2281 cgagatgtat acatatctgg tggatatgga ctatttaagg aagctttgca aatagtagat
     2341 aaaatgtata tcacagaagt agatttaaat attgaagatg gagatacatt ctttccagaa
     2401 tttgatatca atgattttga agttttgata ggggaaacac ttggtgagga agtgaaatat
     2461 acgagaacat tttatgtaag gaaaaaatga attgagtaaa ttttggattt agggtgtttt
     2521 cataaatata ttgcttttta tgcatatata aattgttgta agacaaattc cagtttgtat
     2581 ggggcgataa aatcagaatt tgagagagga ttgaatatga ataaggaatg gtctgaactt
     2641 aataaaacaa tgcaggcaca aataaaaaag aaagatactt ataagagggg tattgatact
     2701 ttacttactt tacgaagtca gttaatacaa accttagtat ctttcaaaga agaattatgc
     2761 agagaagatt ttaactcaat ccccttcata aacgctgatg gttatcatag taagacgatt
     2821 gcttattcta tttggcatat tttccgcatt gaagatatag ttgtgcatac agtgataaat
     2881 gaagatgaac aagtattttt tgcaggcaat tatcaagagc gtatcaattc acctatcatt
     2941 acaacaggaa atgaactcat gaaacagcag attgcagact tttcaaaaca gcttaatctg
     3001 gaagaattat atttatatat tttcgaagta tgggaaagca ctgagaaaat gcttgaacgg
     3061 ttatcttatg atgaattgaa aagaaaaata ccgaaagaaa gaaaaggata cttagaatcg
     3121 ttgaatgtag taaacgacaa tgaaaaagca atttggctga ttgattattg gtgtaataaa
     3181 gatatttgtg gactaattca aatgccgttt tcaagacatt ggattatgc
//
LOCUS       DACEMO010000400         2553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E339
            SAMN07141481-rid9868813.guided.251, whole genome shotgun sequence.
ACCESSION   DACEMO010000400 DACEMO010000000
VERSION     DACEMO010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141481
            Sequence Read Archive: SRR5811455
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740785.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,908
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,828
            CDSs (with protein)               :: 2,828
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 29 of 58
            Pseudo Genes (incomplete)         :: 26 of 58
            Pseudo Genes (internal stop)      :: 12 of 58
            Pseudo Genes (multiple problems)  :: 8 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141481-rid9868813.guided.251"
                     /isolate="E339"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..459
                     /locus_tag="IW728_002906"
     CDS             <1..459
                     /locus_tag="IW728_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1026217.1"
                     /translation="TLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELIDNIRD
                     KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFK
                     GRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            673..1368
                     /gene="vanR"
                     /locus_tag="IW728_002907"
     CDS             673..1368
                     /gene="vanR"
                     /locus_tag="IW728_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1026218.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1346..2500
                     /locus_tag="IW728_002908"
     CDS             1346..2500
                     /locus_tag="IW728_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1026219.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          880 a          454 c          535 g          684 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc tacaggaaga tgacatcatt
       61 tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat
      121 aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca
      181 gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag
      241 cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag
      301 tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag
      361 ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg
      421 gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg
      481 ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc
      541 gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg
      601 taaatgctta taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa
      661 agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat
      721 ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa
      781 gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt
      841 cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt
      901 atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg
      961 gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag
     1021 ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac
     1081 tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc
     1141 cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt
     1201 agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac
     1261 accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg
     1321 aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta
     1381 ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt
     1441 attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat
     1501 tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat
     1561 acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg
     1621 tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt
     1681 acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca
     1741 aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga
     1801 acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc
     1861 cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc
     1921 aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt
     1981 ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct
     2041 atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa
     2101 acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc
     2161 gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat
     2221 tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag
     2281 catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg
     2341 ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca
     2401 gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct
     2461 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taattttggc
     2521 tctttgtcaa atgatgttgg taaagagaaa tct
//
